Bm1_52610 cDNA ORF clone, Brugia malayi

Gene
Clones
gRNAs

Summary

Gene Symbol	Bm1_52610
Entrez Gene ID	6105405
Full Name	RasGEF domain containing protein
Gene Type	protein-coding
Organism	Brugia malayi

mRNA and Protein(s)

mRNA	Protein	Name
XM_001901955.1	XP_001901990.1	RasGEF domain containing protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Draft genome of the filarial nematode parasite Brugia malayi.
Ghedin E, Wang S, Spiro D, Caler E, Zhao Q, Crabtree J, Allen JE, Delcher AL, Guiliano DB, Miranda-Saavedra D, Angiuoli SV, Creasy T, Amedeo P, Haas B, El-Sayed NM, Wortman JR, Feldblyum T, Tallon L, Schatz M, Shumway M, Koo H, Salzberg SL, Schobel S, Pertea M, Pop M, White O, Barton GJ, Carlow CK, Crawford MJ, Daub J, Dimmic MW, Estes CF, Foster JM, Ganatra M, Gregory WF, Johnson NM, Jin J, Komuniecki R, Korf I, Kumar S, Laney S, Li BW, Li W, Lindblom TH, Lustigman S, Ma D, Maina CV, Martin DM, McCarter JP, McReynolds L, Mitreva M, Nutman TB, Parkinson J, Peregrín-Alvarez JM, Poole C, Ren Q, Saunders L, Sluder AE, Smith K, Stanke M, Unnasch TR, Ware J, Wei AD, Weil G, Williams DJ, Zhang Y, Williams SA, Fraser-Liggett C, Slatko B, Blaxter ML, Scott AL
Science (New York, N.Y.)317(5845)1756-60(2007 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Bm1_52610 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bm1_52610 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBd08260	XM_001901955.1 Latest version!	Brugia malayi RasGEF domain containing protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bm1_52610 ( XM_001901955.1 ) cDNA ORF clone, Brugia malayi -> XP_001901990.1 Brugia malayi RasGEF domain containing protein partial mRNA.

Price & Availability↑

CloneID	OBd08260
Clone ID Related Accession (Same CDS sequence)	XM_001901955.1
Accession Version	XM_001901955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1206892800000
Organism	Brugia malayi
Product	RasGEF domain containing protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001893041). COMPLETENESS: incomplete on both ends.

ATGTTTGAGC TAGGAAAGTA TGGTGGAGCC ACACGTCATT CCATGAGCAT CAGCGTGGGT 
GATGCTACCT TTAATCGCTC ATTAGTAGCT AACCGTCAAT CGCAGTGGAA GAGTCAGAAG 
GTATTCATCG ATCGGATATA TCAGATATCC AACAATTGTC ATCCTGGCAT CGGACTGGAC 
CAAGCAGCTG CTGAACATAT CCAAGAAATC TTGATATGTT TATTTTTTGA ATTACTGGAA 
TATCATCCAC AGACAATCGA GGACATGGAA CGAAATATGC GTACCACTTT GCCGGCAAGC 
ATGTTCCATT GGGTTATGCA GTTTCAAAAT ACTCCTGAAG CATTACACAG AATTCCACGG 
AAAAAACTTC ATGATAAACG AAATGCAAAC TCAAAAAGCC TGATGTCACT TTTGCACATG 
TTGCAACCAA AACTGAAGGA ATTGCTGGGT TACAAATTAG ATGAGGAGGT AATGCTATAC 
TTGCTTTCCG TTATCGAATA CATTGCTGCA GATATACTAA AGTGGACTGG AAACTATGTG 
AAAAATATAA GGAAATGTGA TCCCACTATT GGTTTGCAGA ATTTGAAAAT TGCGCTTAGT 
GCTGATACAA GCTTGATGGA GCTTACAGAA ATGTTGTACA ATGAAGAGGA GACATCCACA 
GCCGGAATAT TAAATGATAA TGTCGAAGAT ATTGTGCAAG AAATGTCATA TGATGAGGCA 
TCACGCGATT TCAATCGTGA GGAAGCTCAA TATCTTCGTG ATCTTAATCT CATCATTCAT 
GTCTTTCGGC GTCGTTTTGA GACTGCTTTC GAGCATGATG CACACTGTCT TGATGAAATG 
TTTGGCAACA TTTTGGAATT GCATGAATTA ACAGTGAAGG TTCAGCGAAT GTTAGAAGAT 
GCAATTGAAA TGTCTGATAC TCCATGTGTA GGAGCTGGTT TATGGGAACT TGCGGAAGCT 
CATGAATTTG ATGTTTACAT TGCTTATATG CTGGAAGATC GGACACACAG TATAACTCCT 
GGGGGTGAAG CGTTTCGACT TGCTGTTAAA TACGTGCTTC CTTCTCTTCT CGAAATTCCT 
GTTATTCATT TCTTTCGTTA TGTTGAGTTT ACAAATATTT TATGTCACCT GGCACGACCG 
CCTGATGAAA TCGAAGATTT AAAAAGCACG AAAAGTTATT TTAGCGGTCT TGCTGTAAAA 
ATGGAGTCGC TATGTCCTTT ATTTCTGATT AATCATCTGA AAACGGAACA AAGCGTTCGT 
GCTTTGCCAG AATCAAATTG CTCACGTCAG AGACGAAGGA TCCAAGAAAT TCAGCGAAGC 
ATTGAATTAT GGGAAGGGAA GGAAATTGGT TACAAATGTG CAGAAGTTAT TAGAGAAGGT 
GATTTGTTAC TGTTGCGATC TGGACCACCA TCTTCTGTTG ATACGCTGAA AAAGAACAGG 
GGAATAACTA TGCGTCATAC ATTTCTCTTC GATCATCTTT TGGTGCTTTG TAAAACATTA 
CGCTCTTCAA GGCCAGAGAA ACCACTTTAT AAATTTAAGG ATAAAGTGCT TATACGTAAA 
ACTGATATAT TCGATCTAAA AGATACTGAA GAATTTCAAA ATGCCTTTAA AATTGTATCG 
CGATCGATAA CTCATGAACG AGGAGACAAC ACTTCCTGGA CTTTGCTTTG TCGAACGCCG 
GAAGAGAAAA CTAGTTGGAT GTGCGATTTA GTCAAAATTC AGGCGAAAAG TTCATTAGAT 
CGAATGTTGG ATGCATCGTT GAAAGAAGAA GAAAAACGAG TTCCACTAAT TTTGCCGACA 
TCTGACCAGT ATAGGTTCGC TGATCAAGAC TGCGATGAAA ATATTGTGTT TGAAGATTAT 
ACATCAAGTA GTGGGATACC AGTCGTTAAG CATGGAACTG TTCTAAAACT TGTTGAACGC 
TTAACTTATC ACTTGCAATT GCTCATCGAA CGTTTTCAAG TACCTATTCC GCCACAACTC 
CAAATAGCTG AAGCGAATAG TATATCTAGT TTGGAGTCAC GTGAGCTTTT AAATGGTCCT 
TGTGCCGCAG CACATTCTCA AGGTCTGAGG ATCCAAGTTT CTCCGACAGT GCTAAATAGA 
ATTGAGCGAA GTTATCAGCG ATTTCGCAAA GAATATCAGC AACCAATACA GTGCAGAGTA 
CTAAGTGTGA TTCGACAGTG GGTCAATAAC CACTGGTATG ATTTCGAGCA TAATCCCGTT 
TTGTTGGAAA ACTTATGTTC GTTTTTGGAA GAAACTGATT CTCATGGAAA AGTTATCAAT 
CAGTACAAGA AATGGTGTAA GAGCATTAAG AAACGAGTTG ATATAATACC GTCGGCGACA 
CATAGTGGTT TAGAAAGTGA ACCTTCCCCA GTGGCAACCA CTTCGTATGG TCCACAGAAA 
CCGAAGATTT TGTGGCATAT TGCCGAGAAA GGAGCCATCG AAACGTACGA TCTCTTGTCA 
TTACATCCAA TTGAAATTGG TCGACAGATA ACCCTGTTAC AATTTGACCT TTATAAAGCT 
ATTAAACCCA TCGAATTGGT TGGTTCAGCA TGGACGAAAA GGGATAAAGA TCTTAGAAGT 
CCACAATTGC TTAAGCTCAT CGATCATAGT ACTATGTTAA CTTATTGGGT TGCTCGCTCT 
ATTGTGGAAA CTTGTTCATT GGACGAGCGA GTATATATGT TCTCACGTGT GCTAGAAATA 
ATGTCTGTTT TCGAAGAACT CAACAATTTC ACTGGACTCG TTGCTTTATA TTCTGCTCTG 
AATAGTAGCT CTGTATATCG GCTTAAGGCA TGTTGGGAGA GAATTGATCG GGAAAAGCAA 
GTATGGTATG AAAAATTCAA GAAACTGTGC AATCCGCACT GGAAAGAAAT GATTGAACGA 
TTGAAAAGTA TAAATCCGCC TTGTGTCCCA TTTTTTGGTC ATTATCTTTC CAAAATCTTT 
TTTTACGAGG AAGGGAACAG TACATTTGTA CAGAGCGAAG ATCTCACTCA TGAGCAGATT 
AGTGCTGATG GTAACACATC AGTCGTGGGT GCAACAGGTC GTAAAGTGAT GGTGTCTTTT 
GTGAAATGCC GTCGGATAGC ATCAATTATC AGTGATATCC AAATGTATCA AAATGAATCG 
TATGCATTGG AAGTGGAGCC AAGTATAAGG GTATCAGTTA CTGCCAACGC GGATTTAAAC 
GAATCAATTG AACCAGTAGC AGTTGTGGTA AGAAGCGCCT CTGAGGCCGG CAATTGCAAA 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001901990.1
CDS	1..3246
Translation	MFELGKYGGATRHSMSISVGDATFNRSLVANRQSQWKSQKVFIDRIYQISNNCHPGIGLDQAAAEHIQEILICLFFELLEYHPQTIEDMERNMRTTLPASMFHWVMQFQNTPEALHRIPRKKLHDKRNANSKSLMSLLHMLQPKLKELLGYKLDEEVMLYLLSVIEYIAADILKWTGNYVKNIRKCDPTIGLQNLKIALSADTSLMELTEMLYNEEETSTAGILNDNVEDIVQEMSYDEASRDFNREEAQYLRDLNLIIHVFRRRFETAFEHDAHCLDEMFGNILELHELTVKVQRMLEDAIEMSDTPCVGAGLWELAEAHEFDVYIAYMLEDRTHSITPGGEAFRLAVKYVLPSLLEIPVIHFFRYVEFTNILCHLARPPDEIEDLKSTKSYFSGLAVKMESLCPLFLINHLKTEQSVRALPESNCSRQRRRIQEIQRSIELWEGKEIGYKCAEVIREGDLLLLRSGPPSSVDTLKKNRGITMRHTFLFDHLLVLCKTLRSSRPEKPLYKFKDKVLIRKTDIFDLKDTEEFQNAFKIVSRSITHERGDNTSWTLLCRTPEEKTSWMCDLVKIQAKSSLDRMLDASLKEEEKRVPLILPTSDQYRFADQDCDENIVFEDYTSSSGIPVVKHGTVLKLVERLTYHLQLLIERFQVPIPPQLQIAEANSISSLESRELLNGPCAAAHSQGLRIQVSPTVLNRIERSYQRFRKEYQQPIQCRVLSVIRQWVNNHWYDFEHNPVLLENLCSFLEETDSHGKVINQYKKWCKSIKKRVDIIPSATHSGLESEPSPVATTSYGPQKPKILWHIAEKGAIETYDLLSLHPIEIGRQITLLQFDLYKAIKPIELVGSAWTKRDKDLRSPQLLKLIDHSTMLTYWVARSIVETCSLDERVYMFSRVLEIMSVFEELNNFTGLVALYSALNSSSVYRLKACWERIDREKQVWYEKFKKLCNPHWKEMIERLKSINPPCVPFFGHYLSKIFFYEEGNSTFVQSEDLTHEQISADGNTSVVGATGRKVMVSFVKCRRIASIISDIQMYQNESYALEVEPSIRVSVTANADLNESIEPVAVVVRSASEAGNCKQ

Target ORF information:

RefSeq Version	XM_001901955.1
Organism	Brugia malayi
Definition	Brugia malayi RasGEF domain containing protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001901955.1

ORF Insert Sequence:

ATGTTTGAGC TAGGAAAGTA TGGTGGAGCC ACACGTCATT CCATGAGCAT CAGCGTGGGT 
GATGCTACCT TTAATCGCTC ATTAGTAGCT AACCGTCAAT CGCAGTGGAA GAGTCAGAAG 
GTATTCATCG ATCGGATATA TCAGATATCC AACAATTGTC ATCCTGGCAT CGGACTGGAC 
CAAGCAGCTG CTGAACATAT CCAAGAAATC TTGATATGTT TATTTTTTGA ATTACTGGAA 
TATCATCCAC AGACAATCGA GGACATGGAA CGAAATATGC GTACCACTTT GCCGGCAAGC 
ATGTTCCATT GGGTTATGCA GTTTCAAAAT ACTCCTGAAG CATTACACAG AATTCCACGG 
AAAAAACTTC ATGATAAACG AAATGCAAAC TCAAAAAGCC TGATGTCACT TTTGCACATG 
TTGCAACCAA AACTGAAGGA ATTGCTGGGT TACAAATTAG ATGAGGAGGT AATGCTATAC 
TTGCTTTCCG TTATCGAATA CATTGCTGCA GATATACTAA AGTGGACTGG AAACTATGTG 
AAAAATATAA GGAAATGTGA TCCCACTATT GGTTTGCAGA ATTTGAAAAT TGCGCTTAGT 
GCTGATACAA GCTTGATGGA GCTTACAGAA ATGTTGTACA ATGAAGAGGA GACATCCACA 
GCCGGAATAT TAAATGATAA TGTCGAAGAT ATTGTGCAAG AAATGTCATA TGATGAGGCA 
TCACGCGATT TCAATCGTGA GGAAGCTCAA TATCTTCGTG ATCTTAATCT CATCATTCAT 
GTCTTTCGGC GTCGTTTTGA GACTGCTTTC GAGCATGATG CACACTGTCT TGATGAAATG 
TTTGGCAACA TTTTGGAATT GCATGAATTA ACAGTGAAGG TTCAGCGAAT GTTAGAAGAT 
GCAATTGAAA TGTCTGATAC TCCATGTGTA GGAGCTGGTT TATGGGAACT TGCGGAAGCT 
CATGAATTTG ATGTTTACAT TGCTTATATG CTGGAAGATC GGACACACAG TATAACTCCT 
GGGGGTGAAG CGTTTCGACT TGCTGTTAAA TACGTGCTTC CTTCTCTTCT CGAAATTCCT 
GTTATTCATT TCTTTCGTTA TGTTGAGTTT ACAAATATTT TATGTCACCT GGCACGACCG 
CCTGATGAAA TCGAAGATTT AAAAAGCACG AAAAGTTATT TTAGCGGTCT TGCTGTAAAA 
ATGGAGTCGC TATGTCCTTT ATTTCTGATT AATCATCTGA AAACGGAACA AAGCGTTCGT 
GCTTTGCCAG AATCAAATTG CTCACGTCAG AGACGAAGGA TCCAAGAAAT TCAGCGAAGC 
ATTGAATTAT GGGAAGGGAA GGAAATTGGT TACAAATGTG CAGAAGTTAT TAGAGAAGGT 
GATTTGTTAC TGTTGCGATC TGGACCACCA TCTTCTGTTG ATACGCTGAA AAAGAACAGG 
GGAATAACTA TGCGTCATAC ATTTCTCTTC GATCATCTTT TGGTGCTTTG TAAAACATTA 
CGCTCTTCAA GGCCAGAGAA ACCACTTTAT AAATTTAAGG ATAAAGTGCT TATACGTAAA 
ACTGATATAT TCGATCTAAA AGATACTGAA GAATTTCAAA ATGCCTTTAA AATTGTATCG 
CGATCGATAA CTCATGAACG AGGAGACAAC ACTTCCTGGA CTTTGCTTTG TCGAACGCCG 
GAAGAGAAAA CTAGTTGGAT GTGCGATTTA GTCAAAATTC AGGCGAAAAG TTCATTAGAT 
CGAATGTTGG ATGCATCGTT GAAAGAAGAA GAAAAACGAG TTCCACTAAT TTTGCCGACA 
TCTGACCAGT ATAGGTTCGC TGATCAAGAC TGCGATGAAA ATATTGTGTT TGAAGATTAT 
ACATCAAGTA GTGGGATACC AGTCGTTAAG CATGGAACTG TTCTAAAACT TGTTGAACGC 
TTAACTTATC ACTTGCAATT GCTCATCGAA CGTTTTCAAG TACCTATTCC GCCACAACTC 
CAAATAGCTG AAGCGAATAG TATATCTAGT TTGGAGTCAC GTGAGCTTTT AAATGGTCCT 
TGTGCCGCAG CACATTCTCA AGGTCTGAGG ATCCAAGTTT CTCCGACAGT GCTAAATAGA 
ATTGAGCGAA GTTATCAGCG ATTTCGCAAA GAATATCAGC AACCAATACA GTGCAGAGTA 
CTAAGTGTGA TTCGACAGTG GGTCAATAAC CACTGGTATG ATTTCGAGCA TAATCCCGTT 
TTGTTGGAAA ACTTATGTTC GTTTTTGGAA GAAACTGATT CTCATGGAAA AGTTATCAAT 
CAGTACAAGA AATGGTGTAA GAGCATTAAG AAACGAGTTG ATATAATACC GTCGGCGACA 
CATAGTGGTT TAGAAAGTGA ACCTTCCCCA GTGGCAACCA CTTCGTATGG TCCACAGAAA 
CCGAAGATTT TGTGGCATAT TGCCGAGAAA GGAGCCATCG AAACGTACGA TCTCTTGTCA 
TTACATCCAA TTGAAATTGG TCGACAGATA ACCCTGTTAC AATTTGACCT TTATAAAGCT 
ATTAAACCCA TCGAATTGGT TGGTTCAGCA TGGACGAAAA GGGATAAAGA TCTTAGAAGT 
CCACAATTGC TTAAGCTCAT CGATCATAGT ACTATGTTAA CTTATTGGGT TGCTCGCTCT 
ATTGTGGAAA CTTGTTCATT GGACGAGCGA GTATATATGT TCTCACGTGT GCTAGAAATA 
ATGTCTGTTT TCGAAGAACT CAACAATTTC ACTGGACTCG TTGCTTTATA TTCTGCTCTG 
AATAGTAGCT CTGTATATCG GCTTAAGGCA TGTTGGGAGA GAATTGATCG GGAAAAGCAA 
GTATGGTATG AAAAATTCAA GAAACTGTGC AATCCGCACT GGAAAGAAAT GATTGAACGA 
TTGAAAAGTA TAAATCCGCC TTGTGTCCCA TTTTTTGGTC ATTATCTTTC CAAAATCTTT 
TTTTACGAGG AAGGGAACAG TACATTTGTA CAGAGCGAAG ATCTCACTCA TGAGCAGATT 
AGTGCTGATG GTAACACATC AGTCGTGGGT GCAACAGGTC GTAAAGTGAT GGTGTCTTTT 
GTGAAATGCC GTCGGATAGC ATCAATTATC AGTGATATCC AAATGTATCA AAATGAATCG 
TATGCATTGG AAGTGGAGCC AAGTATAAGG GTATCAGTTA CTGCCAACGC GGATTTAAAC 
GAATCAATTG AACCAGTAGC AGTTGTGGTA AGAAGCGCCT CTGAGGCCGG CAATTGCAAA 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Draft genome of the filarial nematode parasite Brugia malayi.
Science (New York, N.Y.)317(5845)1756-60(2007 Sep)
Ghedin E,Wang S,Spiro D,Caler E,Zhao Q,Crabtree J,Allen JE,Delcher AL,Guiliano DB,Miranda-Saavedra D,Angiuoli SV,Creasy T,Amedeo P,Haas B,El-Sayed NM,Wortman JR,Feldblyum T,Tallon L,Schatz M,Shumway M,Koo H,Salzberg SL,Schobel S,Pertea M,Pop M,White O,Barton GJ,Carlow CK,Crawford MJ,Daub J,Dimmic MW,Estes CF,Foster JM,Ganatra M,Gregory WF,Johnson NM,Jin J,Komuniecki R,Korf I,Kumar S,Laney S,Li BW,Li W,Lindblom TH,Lustigman S,Ma D,Maina CV,Martin DM,McCarter JP,McReynolds L,Mitreva M,Nutman TB,Parkinson J,Peregrín-Alvarez JM,Poole C,Ren Q,Saunders L,Sluder AE,Smith K,Stanke M,Unnasch TR,Ware J,Wei AD,Weil G,Williams DJ,Zhang Y,Williams SA,Fraser-Liggett C,Slatko B,Blaxter ML,Scott AL

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Bm1_52610 cDNA ORF clone, Brugia malayi

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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