BRAFLDRAFT_101341 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_101341
Entrez Gene ID	7255995
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002603793.1	XP_002603839.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_101341 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_101341 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb20922	XM_002603793.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_101341 ( XM_002603793.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002603839.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb20922
Clone ID Related Accession (Same CDS sequence)	XM_002603793.1
Accession Version	XM_002603793.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101505). COMPLETENESS: incomplete on both ends.

ATGGCCAGCC CAACCCACCA GGCAGCCGCT GAAGCACCTC CAGATTCCGA CACGAACTTC 
ACACCAGACG AAGCAGCTGC GGCCAGATCC GGCGGGGCAT CCGATGGAGG GGACCCGGAC 
AGCACCGTCG ATACTGCCGA GGGTGCCAAA CCAACAGCCT TTGAGAAGGG CGATCAAAAT 
TCTTTACAGC TCGGCAAACC TGTGGGCGGT AGTGCTGTGT CAACTGGAAA CAGAGCTGCC 
CCGGCCGGAC ACAAGCTGAT CCTGGTGGCC GATGCAACGG ACAGGTCCAT CAGTCGGCTA 
ACTACGGAAG ATGATATCCA ATTTCAGTGG GGTCGCTATG AAATCGACAA TGATTTTCAT 
AGACCAATAG CTGTTGATTA CGACCGGACG GAGGATTTTA TCTACTGGAC CAGTGTTAAC 
GGCGCGAATA GTGTTGTGTT CCGTTCTCCC TACGAGACTG GAACGGGGCT GAGACTTGAT 
GGACAGCAGG CTTCAGTTCC TGACGGTATT GCAGTAGACG TCATTTCCAG GAACCTGTAC 
TGGACAGACA CCGGTACAGA CCGCATCATC GTCTCCCGCC TGGACGGCAG CTTCAGGAAG 
TCCCTCATAA CCCAGGGGCT GGACGAACCC AGGGCGATCG TAGTGGATCC CAACTCGGGT 
TGGATGTACT GGACCGACTG GGGAAACCCG TCTAAGATTG AGCGTGCGCA AATGGACGGT 
ACTCAGAGGT CTGTTATTGT CAATGTCGTG CAGGGCCACT GGCCAAACGG ACTGGCCTTG 
GATGCAGCAG AAAACCGGCT GTACTGGTGT AACGGTGGTA CTGGCGCGAT CTGGACCTCT 
GGAGTGACTG GCAGCAACCC GAGCCCCACA CAATTGTTTA CGCAGTCAGC CGGTGACGTA 
AATGCACACC TGTTCGGGAT CGCTGTGGAT GAGACACATC TCTACTGGAC AGCGTGGAAC 
ATACCGGGCG TCCACAGGAT TAGCAAGTCG TTGACTGGAT ACGTCAGGCT CAACCTGCCA 
CACTTTCAAA AGCTGTATGA CATCCACGTC AGGACAGACT CAAACACTCC GAGTCAACCA 
AACGCCTGCT CTGTGTCTAA CGGGAACTGT GCCCAGCTGT GTCTCCCCGT CCCCGGCGGA 
GGGTGGACCT GCGCCTGTCA GGATGGGTGG AGCCTGGGGT CGGATGGACG GTCCTGTATT 
TCGGATACCT GTCTGCCTAA CCCATGTCAA AATGGTGGCC AGTGTATCGG TGGGGACGAC 
TCTTACAGCT GCGATTGTCT TGATGGATTC AGTGGAGTCA ACTGCCAGAC CAATATCAAC 
GAGTGCGCAT CGGGTCCATG TCAAAATGGT GGCCAATGTC AAGATGGGGA CAACTCTTAC 
ACCTGCGATT GTCCGGATGG CTTCAGTGGA GTCAACTGCC AGATCAATAT CAACGAGTGC 
GCATCGGGTC CATGTCAAAA TGGTGGCCAA TGTCAAGACG GGGACAACTC TTACACCTGC 
GATTGTCCGG ATGGCTTCAA TGGAGTCAAC TGCCTGATCA ATATCAACGA GTGCGCATCG 
GGTCCATGTC AAAATGGTGG CCAATGTCAA GACGGGGACA ACTCTTACAC CTGCGATTGT 
CCGGATGGCT TCAGTGGAGT CAACTGCCAG ATCAATATCA ACGAGTGCGC ATCGGGTCCA 
TGTCAAAATG GTGGCCAATG TCAAGATGGG GACAACTCTT ACACCTGCGA TTGTCCGGAT 
GGCTTCAGTG GAGTCAACTG CCAGATCAAT ATCAACGAGT GCGCATCGGG TCCATGTCAA 
AATGGTGGCC AATGTCAAGA TGGGGACAAC TCTTACACCT GCGATTGTCC GGATGGCTTC 
AGTGGAGTCA ACTGCCAGAT CAATATCAAC GAGTGCGCAT CGGGTCCATG TCAAAATGGT 
GGCCAATGTC AAGATGGGGA CAACTCTTAC AGCTGCGATT GTCCGGATGG CTTCAGTGGA 
GTCAACTGCC AGATCAATAT CAACGATGGA GTCAACTGCC AGACCAATAT CAACGAGTGC 
GCATCGGGTC CATGTCAAAA TGGTGGCCAA TGTCAAGACG GGGACAACTC TTACACCTGC 
GATTGTCCGG ATGGCTTCAG TGGAGTCAAC TGCCAGACCA ATATCAACGA GTGCGCATCG 
GGTCCATGTC AAAATGGTGG CCAATGTCAA GACGGGGACA ACTCTTACAC CTGCGATTGT 
CCGGATGGCT TCAGTGGAGT CAACTGCCAG ATCAATATCA ACGAGTGCGC ATCGGGTCCA 
TGTCAAAATG GTGGCCAATG TCAAGACGGG GACAACTCTT ACACCTGCGA TTGTCCGGAT 
GGCTTCAGTG GAGTCAACTG CCAGATCAAT ATCAACGAGT GCGCATCGGG TCCATGTCAA 
AATGGTGGCC AATGTCAAGA CGGGGACAAC TCTTACACCT GCGATTGTCC GGATGGCTTC 
AGTGGAGTCA ACTGCCAGAT CAATATCAAC GAGTGCGCAT CGGGTCCATG TCAAAATGGT 
GGCCAATGTC AAGACGGGGA CAACTCTTAC ACCTGCGATT GTCCGGATGG CTTCAGTGGA 
GTCAACTGCC TGATCAATAT CAACGAGTAC GTTGACGGTG ACCTTGTCAG CGATCCTTTC 
AACGAGTGGG CAGATGGAAC TTGGAACAGC AGGAAGAAAG ACTGTGTGCG CATGAGCGAA 
ACCTTCGGGT ACCATTGGCA GCCAATGCCG TGCGGCACGC GGCTGTCGTA CATTTGCGAG 
TATCATGTTG AACGTTGTGA GCCGGAGACC TGTAACCAGG GAGGGATCTG TATAGACGGT 
CCGTCGTCCT ACACCTGTTA TTGTCTGCCG GGGTTTCTGG GAGACCACTG TGAGACAGCT 
TGCCCTGTCG GATTTACGCT GTTCAAGGAC CAGTGCTACT GGTTCTCTCC AGCTTCCCTG 
CGGAAAACCG TGGCAGCTGC AGAAACAGAC TGTGATGCCC GAGACGCCCG TCTGGCTTGT 
GTGAAGGACG AAGACACGCA CAACTTCCTC AAAATGACTA TCGCTACAAC AAACCGCAGG 
CCTTACTGGA TCGGGTTGAG TGACAGGGTA GTGGAGGGTA CATGGCGCCA CAGTGACGGG 
ACAGAGCTGG GCAGTTTCCG GCCTTTCCGT CCGTCCCGTA ACACCAACAA TGTGTGGAGG 
GACTGCGTTC TGATGTGGCC AGCCTCTTCC TACCAGTGGC TGTACTATAG CTGTAATTCT 
CGCAGGAACT TCGTCTGCCA GAGGGCCCAC CAGGCAGCCG CTGAAGCACC TCCAGATTCC 
GACACGAACT TCACACCAGA CGAAGCAGCT GCGGCCAGAT CCGGCGGGGA ATCCGAGGCA 
GAGGACCCGG ACAGCACAGT CGACACTGCC GAGGGTGCCA AACCAGCAAC CTTTGAGAAG 
GGCGATTTAG ATTCTCTACA GCTCGGCGAG CCTGTAGACG GTGATGCTGT GTCGGCTGGA 
AACAGAGCTG CCCCGGCCGG ACACAAGCTG ATCCTGGTGG CCGATGCAAC GGACAGGACC 
ATCACTCGGC TAACAACAGA CGATGATAAC CAATTTCAGT GGGGTCGCTA TGACATCGGG 
AATGGTACCA ACAGACCAAT AGCTGTGGAT TATGATCGTG CGGAGGACTT TATCTACTGG 
ACCAGTGTTA ACGGACCCTA TAGTAGGGTA TCCCGATTTC CCTACGAGAG CGGATCGGGA 
CTGAGACTTG ATGGAGGGCA GGCTTCAGTT CCTGAGGGCA TTTCAGTGGA CGTCATTTCC 
AGGAACCTGT ACTGGACAGA CACCGGTACA GACCGCATCA TCGTCTCCCG CTTGGACGGC 
AGCTTCAGGA AGTCCCTCAT CACCCAGGGG CTGGACGAGC CCAGGGCGAT CGTAGTGGAC 
CCCAACTCGG GGATAAGCAA GTCTCTGACT GGATACGTCA GGCTCAACCT GCCACACTTT 
CAAAAGCTGT ATGACATCCA CGTCAGGACA GACTCAAACA CTCCGAGTCA ACCAAACGCC 
TGCTCTGTGT CTAACGGGAA CTGTGCCCAG CTGTGTCTTC CCGTCCCCGG CGGAGGGCGG 
ACCTGCGCCT GTCAGGATGG GTGGAGCCTG GGGTCGGATG GACGGTCCTG TCAGGCCGAC 
GCCGGCTTTA GTGGCGATAA ATGCGAGATC AACGAAAACG AGTGTGTGTC AAATCCATGT 
CAAAATGGCG GCCAGTGTCA AGACGAGGCA AACTCTTACA GCTGTACCTG TGCAGCTGGC 
TACAGTGGAG ACGACTGTGA GACCAATGAC GACGACTGTG CACCCAATCC ATGTCAAAAT 
GGTGGACAAT GTCAAGACGG AGTCAACTCT TACACCTGTA CCTGTCCAGC TGGCTACAGT 
GGAGACGACT GTGAGACCAA TGACGACGAC TGTGCACCCA ATCCATGTCA AAATGGCGGC 
CAGTGTCAAG ACGGAGTCAA CTCTTACACC TGTACCTGTG CAGCTGGCTA CAGTGGAGAC 
GACTGTGAGA CCAATGACGA CGACTGTGCA CCCAATCCAT GTCAAAATGG CGGCCAATGT 
CAAGACGGAG TCAACTCTTA CACCTGTACC TGTGCAGCTG GCTACAGTGG AGACGACTGT 
GAGACCAATG ACGACGACTG TGCACCCAAT CCATGTCAAA ATGGCGGCCA GTGTCAAGAC 
GGAGTCAACT CTTACACCTG TTCCTGTGCA GCTGGCTACA GTGGAGACGA CTGTGAGACC 
AATGACGACG ACTGTGCACC CAATCCATGT CAAAATGGCG GCCAGTGTCA AGACGGAGTC 
AACTCTTACA CCTGTTCCTG TGCAGCTGGC TACAGTGGAG ACGACTGTGA GACCAATGAC 
GACGACTGTG CACCCAATCC ATGTCAAAAT GGCGGCCAGT GTCAAGACGG AGTCAACTCT 
TACACCTGCA ATTGTCCAGC TGGCTACAGT GGAGACGACT GTGAGACCAA TGACGACGAC 
TGTGCACCCA ATCCATGTCA AAATGGCGGC CAGTGTCAAG ACGGAGTCAA CTCTTACACC 
TGTAATTGTC CAGCTGGCTA CAGTGGAGAC GACTGTGAGA CCAATGACGA CGACTGTGCA 
CCCAATCCAT GTCAAAATGG CGGCCAGTGT CAAGACGGAG TCAACTCTTA CACCTGTAAC 
TGTCCAGCTG GCTACAGTGG AGACGACTGT GAGACCAATG ACGACGACTG TGCACCCAAT 
CCATGTCAAA ATGGCGGCCA GTGTCAAGAC GGAGTCAACT CTTACACCTG TAACTGTCCA 
GCTGGCTACA GTGGAGACGA CTGTGAGACC AATGACGACG ACTGTGCACC CAATCCATGT 
CAAAATGGCG GCCAGTGTCA AGACGGAGTC AACTCTTACA CCTGCAATTG TCCAGCTGGC 
TACAGTGGAG ACGACTGTGA GACCAATGAC GACGACTGTG CACCCAATCC ATGTCAAAAT 
GGCGGCCAGT GTCAAGACGG AGTCAACTCT TACACCTGTA CTTGTGCAGC TGGCTACAGT 
GGAGACGACT GTGAGATCAA TGACAACGAC TGTAATCCCA ATCCATGTCA AAATGGCGGC 
CAGTGTCAAG ACGGAGTCAA CTCTTACACC TGTGATTGTC CGGATGGCTT CAATGGATTC 
AACTGTCAGA CCAATATCAA CGAGTGCGCA TCTCGTCCAT GTCAAAATGG CGGCCAGTGT 
CAAGACGGTG TCAACTCCTA CACATGTGAT TGTCCTGATG GATATTTGGG AGAACACTGT 
GAAATTTCTG CGACAGACCC TCCACAGACG ACTCCTCAGG GGACAAGTAC CGTGTGTGAA 
TCTGGTTGGT TCCACCATGA GGGGAAGTGC TATTGTCTCC TTGATGACCA GTCCACCTAC 
AGCGAAGCTG CCCGGAGCTG CGCTGAGTTG GACGTCTACG GGCAGCTGGC CGTGGTGAAG 
CGGCCGAGCA CGCACCGCTT CCTGATGGAT TATGTCGAAC GGGAAGGTGG GGGTTCTCCG 
TGGATAGGAA TCAACGATGG CACTACAGAG GGTGTATGGA GGTACAGCAA TGGTGACCTT 
GTCAGTGATC CTTTCAACGA GTGGGCAGAT GGAACTTGGA ACAGCAGGAA GAAAGACTGT 
GTGCGCATGA GCGAAACCTT CGGGTACCAT TGGCAGCCTA TGCCGTGCGA CACGCAGCTG 
TCGTATATTT GCGAGTATCC TCTCCTGCCA CCCACAGATG CTCCAACCCC AACCACACCA 
GATGTTGAAC GTTGTGAGCC GGACACCTGT AACCAGGGAG GGATCTGTAT AGACGGACCG 
TCGTCCTACA CCTGTTACTG TCTGCCGGGG TTTACGGGAG ACCACTGTGA GACAGCTTGC 
CCTGCCGGAT TTACGCTGTT CCAGAAACAG TGCTACTGGT TCTCTCCAAC TTCCATGAGG 
GAAACCGTGG CAAATGCAGA AACAGACTGT GATGCCCGAG GTGCCCGTCT GGCTTGTGTG 
AAGGACGAAG ACACGCACAA CTTCCTCAAA TTGACCATCG CTACAACAAA CCGCAGGCCT 
TACTGGATCG GGTTGAATGA CACGGTGGTG GAGGATGTAT GGCGCCACAG TGACGGGACA 
CTTCTGGGCA GTTTCCGGCC TTTCCGCCCG TCCCGTAACA CCAACAATGT GTGGAGGGAC 
TGCGTTCTGA TGTGGCCAGC CTCTTCCTAC CAGTGGCTGT ACTATAACTG TAATTCTCGC 
AGGAACTACG TCTGCCAGAG GGCTGCAACT CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002603839.1
CDS	1..6756
Translation	MASPTHQAAAEAPPDSDTNFTPDEAAAARSGGASDGGDPDSTVDTAEGAKPTAFEKGDQNSLQLGKPVGGSAVSTGNRAAPAGHKLILVADATDRSISRLTTEDDIQFQWGRYEIDNDFHRPIAVDYDRTEDFIYWTSVNGANSVVFRSPYETGTGLRLDGQQASVPDGIAVDVISRNLYWTDTGTDRIIVSRLDGSFRKSLITQGLDEPRAIVVDPNSGWMYWTDWGNPSKIERAQMDGTQRSVIVNVVQGHWPNGLALDAAENRLYWCNGGTGAIWTSGVTGSNPSPTQLFTQSAGDVNAHLFGIAVDETHLYWTAWNIPGVHRISKSLTGYVRLNLPHFQKLYDIHVRTDSNTPSQPNACSVSNGNCAQLCLPVPGGGWTCACQDGWSLGSDGRSCISDTCLPNPCQNGGQCIGGDDSYSCDCLDGFSGVNCQTNINECASGPCQNGGQCQDGDNSYTCDCPDGFSGVNCQININECASGPCQNGGQCQDGDNSYTCDCPDGFNGVNCLININECASGPCQNGGQCQDGDNSYTCDCPDGFSGVNCQININECASGPCQNGGQCQDGDNSYTCDCPDGFSGVNCQININECASGPCQNGGQCQDGDNSYTCDCPDGFSGVNCQININECASGPCQNGGQCQDGDNSYSCDCPDGFSGVNCQININDGVNCQTNINECASGPCQNGGQCQDGDNSYTCDCPDGFSGVNCQTNINECASGPCQNGGQCQDGDNSYTCDCPDGFSGVNCQININECASGPCQNGGQCQDGDNSYTCDCPDGFSGVNCQININECASGPCQNGGQCQDGDNSYTCDCPDGFSGVNCQININECASGPCQNGGQCQDGDNSYTCDCPDGFSGVNCLININEYVDGDLVSDPFNEWADGTWNSRKKDCVRMSETFGYHWQPMPCGTRLSYICEYHVERCEPETCNQGGICIDGPSSYTCYCLPGFLGDHCETACPVGFTLFKDQCYWFSPASLRKTVAAAETDCDARDARLACVKDEDTHNFLKMTIATTNRRPYWIGLSDRVVEGTWRHSDGTELGSFRPFRPSRNTNNVWRDCVLMWPASSYQWLYYSCNSRRNFVCQRAHQAAAEAPPDSDTNFTPDEAAAARSGGESEAEDPDSTVDTAEGAKPATFEKGDLDSLQLGEPVDGDAVSAGNRAAPAGHKLILVADATDRTITRLTTDDDNQFQWGRYDIGNGTNRPIAVDYDRAEDFIYWTSVNGPYSRVSRFPYESGSGLRLDGGQASVPEGISVDVISRNLYWTDTGTDRIIVSRLDGSFRKSLITQGLDEPRAIVVDPNSGISKSLTGYVRLNLPHFQKLYDIHVRTDSNTPSQPNACSVSNGNCAQLCLPVPGGGRTCACQDGWSLGSDGRSCQADAGFSGDKCEINENECVSNPCQNGGQCQDEANSYSCTCAAGYSGDDCETNDDDCAPNPCQNGGQCQDGVNSYTCTCPAGYSGDDCETNDDDCAPNPCQNGGQCQDGVNSYTCTCAAGYSGDDCETNDDDCAPNPCQNGGQCQDGVNSYTCTCAAGYSGDDCETNDDDCAPNPCQNGGQCQDGVNSYTCSCAAGYSGDDCETNDDDCAPNPCQNGGQCQDGVNSYTCSCAAGYSGDDCETNDDDCAPNPCQNGGQCQDGVNSYTCNCPAGYSGDDCETNDDDCAPNPCQNGGQCQDGVNSYTCNCPAGYSGDDCETNDDDCAPNPCQNGGQCQDGVNSYTCNCPAGYSGDDCETNDDDCAPNPCQNGGQCQDGVNSYTCNCPAGYSGDDCETNDDDCAPNPCQNGGQCQDGVNSYTCNCPAGYSGDDCETNDDDCAPNPCQNGGQCQDGVNSYTCTCAAGYSGDDCEINDNDCNPNPCQNGGQCQDGVNSYTCDCPDGFNGFNCQTNINECASRPCQNGGQCQDGVNSYTCDCPDGYLGEHCEISATDPPQTTPQGTSTVCESGWFHHEGKCYCLLDDQSTYSEAARSCAELDVYGQLAVVKRPSTHRFLMDYVEREGGGSPWIGINDGTTEGVWRYSNGDLVSDPFNEWADGTWNSRKKDCVRMSETFGYHWQPMPCDTQLSYICEYPLLPPTDAPTPTTPDVERCEPDTCNQGGICIDGPSSYTCYCLPGFTGDHCETACPAGFTLFQKQCYWFSPTSMRETVANAETDCDARGARLACVKDEDTHNFLKLTIATTNRRPYWIGLNDTVVEDVWRHSDGTLLGSFRPFRPSRNTNNVWRDCVLMWPASSYQWLYYNCNSRRNYVCQRAATP

Target ORF information:

RefSeq Version	XM_002603793.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002603793.1

ORF Insert Sequence:

ATGGCCAGCC CAACCCACCA GGCAGCCGCT GAAGCACCTC CAGATTCCGA CACGAACTTC 
ACACCAGACG AAGCAGCTGC GGCCAGATCC GGCGGGGCAT CCGATGGAGG GGACCCGGAC 
AGCACCGTCG ATACTGCCGA GGGTGCCAAA CCAACAGCCT TTGAGAAGGG CGATCAAAAT 
TCTTTACAGC TCGGCAAACC TGTGGGCGGT AGTGCTGTGT CAACTGGAAA CAGAGCTGCC 
CCGGCCGGAC ACAAGCTGAT CCTGGTGGCC GATGCAACGG ACAGGTCCAT CAGTCGGCTA 
ACTACGGAAG ATGATATCCA ATTTCAGTGG GGTCGCTATG AAATCGACAA TGATTTTCAT 
AGACCAATAG CTGTTGATTA CGACCGGACG GAGGATTTTA TCTACTGGAC CAGTGTTAAC 
GGCGCGAATA GTGTTGTGTT CCGTTCTCCC TACGAGACTG GAACGGGGCT GAGACTTGAT 
GGACAGCAGG CTTCAGTTCC TGACGGTATT GCAGTAGACG TCATTTCCAG GAACCTGTAC 
TGGACAGACA CCGGTACAGA CCGCATCATC GTCTCCCGCC TGGACGGCAG CTTCAGGAAG 
TCCCTCATAA CCCAGGGGCT GGACGAACCC AGGGCGATCG TAGTGGATCC CAACTCGGGT 
TGGATGTACT GGACCGACTG GGGAAACCCG TCTAAGATTG AGCGTGCGCA AATGGACGGT 
ACTCAGAGGT CTGTTATTGT CAATGTCGTG CAGGGCCACT GGCCAAACGG ACTGGCCTTG 
GATGCAGCAG AAAACCGGCT GTACTGGTGT AACGGTGGTA CTGGCGCGAT CTGGACCTCT 
GGAGTGACTG GCAGCAACCC GAGCCCCACA CAATTGTTTA CGCAGTCAGC CGGTGACGTA 
AATGCACACC TGTTCGGGAT CGCTGTGGAT GAGACACATC TCTACTGGAC AGCGTGGAAC 
ATACCGGGCG TCCACAGGAT TAGCAAGTCG TTGACTGGAT ACGTCAGGCT CAACCTGCCA 
CACTTTCAAA AGCTGTATGA CATCCACGTC AGGACAGACT CAAACACTCC GAGTCAACCA 
AACGCCTGCT CTGTGTCTAA CGGGAACTGT GCCCAGCTGT GTCTCCCCGT CCCCGGCGGA 
GGGTGGACCT GCGCCTGTCA GGATGGGTGG AGCCTGGGGT CGGATGGACG GTCCTGTATT 
TCGGATACCT GTCTGCCTAA CCCATGTCAA AATGGTGGCC AGTGTATCGG TGGGGACGAC 
TCTTACAGCT GCGATTGTCT TGATGGATTC AGTGGAGTCA ACTGCCAGAC CAATATCAAC 
GAGTGCGCAT CGGGTCCATG TCAAAATGGT GGCCAATGTC AAGATGGGGA CAACTCTTAC 
ACCTGCGATT GTCCGGATGG CTTCAGTGGA GTCAACTGCC AGATCAATAT CAACGAGTGC 
GCATCGGGTC CATGTCAAAA TGGTGGCCAA TGTCAAGACG GGGACAACTC TTACACCTGC 
GATTGTCCGG ATGGCTTCAA TGGAGTCAAC TGCCTGATCA ATATCAACGA GTGCGCATCG 
GGTCCATGTC AAAATGGTGG CCAATGTCAA GACGGGGACA ACTCTTACAC CTGCGATTGT 
CCGGATGGCT TCAGTGGAGT CAACTGCCAG ATCAATATCA ACGAGTGCGC ATCGGGTCCA 
TGTCAAAATG GTGGCCAATG TCAAGATGGG GACAACTCTT ACACCTGCGA TTGTCCGGAT 
GGCTTCAGTG GAGTCAACTG CCAGATCAAT ATCAACGAGT GCGCATCGGG TCCATGTCAA 
AATGGTGGCC AATGTCAAGA TGGGGACAAC TCTTACACCT GCGATTGTCC GGATGGCTTC 
AGTGGAGTCA ACTGCCAGAT CAATATCAAC GAGTGCGCAT CGGGTCCATG TCAAAATGGT 
GGCCAATGTC AAGATGGGGA CAACTCTTAC AGCTGCGATT GTCCGGATGG CTTCAGTGGA 
GTCAACTGCC AGATCAATAT CAACGATGGA GTCAACTGCC AGACCAATAT CAACGAGTGC 
GCATCGGGTC CATGTCAAAA TGGTGGCCAA TGTCAAGACG GGGACAACTC TTACACCTGC 
GATTGTCCGG ATGGCTTCAG TGGAGTCAAC TGCCAGACCA ATATCAACGA GTGCGCATCG 
GGTCCATGTC AAAATGGTGG CCAATGTCAA GACGGGGACA ACTCTTACAC CTGCGATTGT 
CCGGATGGCT TCAGTGGAGT CAACTGCCAG ATCAATATCA ACGAGTGCGC ATCGGGTCCA 
TGTCAAAATG GTGGCCAATG TCAAGACGGG GACAACTCTT ACACCTGCGA TTGTCCGGAT 
GGCTTCAGTG GAGTCAACTG CCAGATCAAT ATCAACGAGT GCGCATCGGG TCCATGTCAA 
AATGGTGGCC AATGTCAAGA CGGGGACAAC TCTTACACCT GCGATTGTCC GGATGGCTTC 
AGTGGAGTCA ACTGCCAGAT CAATATCAAC GAGTGCGCAT CGGGTCCATG TCAAAATGGT 
GGCCAATGTC AAGACGGGGA CAACTCTTAC ACCTGCGATT GTCCGGATGG CTTCAGTGGA 
GTCAACTGCC TGATCAATAT CAACGAGTAC GTTGACGGTG ACCTTGTCAG CGATCCTTTC 
AACGAGTGGG CAGATGGAAC TTGGAACAGC AGGAAGAAAG ACTGTGTGCG CATGAGCGAA 
ACCTTCGGGT ACCATTGGCA GCCAATGCCG TGCGGCACGC GGCTGTCGTA CATTTGCGAG 
TATCATGTTG AACGTTGTGA GCCGGAGACC TGTAACCAGG GAGGGATCTG TATAGACGGT 
CCGTCGTCCT ACACCTGTTA TTGTCTGCCG GGGTTTCTGG GAGACCACTG TGAGACAGCT 
TGCCCTGTCG GATTTACGCT GTTCAAGGAC CAGTGCTACT GGTTCTCTCC AGCTTCCCTG 
CGGAAAACCG TGGCAGCTGC AGAAACAGAC TGTGATGCCC GAGACGCCCG TCTGGCTTGT 
GTGAAGGACG AAGACACGCA CAACTTCCTC AAAATGACTA TCGCTACAAC AAACCGCAGG 
CCTTACTGGA TCGGGTTGAG TGACAGGGTA GTGGAGGGTA CATGGCGCCA CAGTGACGGG 
ACAGAGCTGG GCAGTTTCCG GCCTTTCCGT CCGTCCCGTA ACACCAACAA TGTGTGGAGG 
GACTGCGTTC TGATGTGGCC AGCCTCTTCC TACCAGTGGC TGTACTATAG CTGTAATTCT 
CGCAGGAACT TCGTCTGCCA GAGGGCCCAC CAGGCAGCCG CTGAAGCACC TCCAGATTCC 
GACACGAACT TCACACCAGA CGAAGCAGCT GCGGCCAGAT CCGGCGGGGA ATCCGAGGCA 
GAGGACCCGG ACAGCACAGT CGACACTGCC GAGGGTGCCA AACCAGCAAC CTTTGAGAAG 
GGCGATTTAG ATTCTCTACA GCTCGGCGAG CCTGTAGACG GTGATGCTGT GTCGGCTGGA 
AACAGAGCTG CCCCGGCCGG ACACAAGCTG ATCCTGGTGG CCGATGCAAC GGACAGGACC 
ATCACTCGGC TAACAACAGA CGATGATAAC CAATTTCAGT GGGGTCGCTA TGACATCGGG 
AATGGTACCA ACAGACCAAT AGCTGTGGAT TATGATCGTG CGGAGGACTT TATCTACTGG 
ACCAGTGTTA ACGGACCCTA TAGTAGGGTA TCCCGATTTC CCTACGAGAG CGGATCGGGA 
CTGAGACTTG ATGGAGGGCA GGCTTCAGTT CCTGAGGGCA TTTCAGTGGA CGTCATTTCC 
AGGAACCTGT ACTGGACAGA CACCGGTACA GACCGCATCA TCGTCTCCCG CTTGGACGGC 
AGCTTCAGGA AGTCCCTCAT CACCCAGGGG CTGGACGAGC CCAGGGCGAT CGTAGTGGAC 
CCCAACTCGG GGATAAGCAA GTCTCTGACT GGATACGTCA GGCTCAACCT GCCACACTTT 
CAAAAGCTGT ATGACATCCA CGTCAGGACA GACTCAAACA CTCCGAGTCA ACCAAACGCC 
TGCTCTGTGT CTAACGGGAA CTGTGCCCAG CTGTGTCTTC CCGTCCCCGG CGGAGGGCGG 
ACCTGCGCCT GTCAGGATGG GTGGAGCCTG GGGTCGGATG GACGGTCCTG TCAGGCCGAC 
GCCGGCTTTA GTGGCGATAA ATGCGAGATC AACGAAAACG AGTGTGTGTC AAATCCATGT 
CAAAATGGCG GCCAGTGTCA AGACGAGGCA AACTCTTACA GCTGTACCTG TGCAGCTGGC 
TACAGTGGAG ACGACTGTGA GACCAATGAC GACGACTGTG CACCCAATCC ATGTCAAAAT 
GGTGGACAAT GTCAAGACGG AGTCAACTCT TACACCTGTA CCTGTCCAGC TGGCTACAGT 
GGAGACGACT GTGAGACCAA TGACGACGAC TGTGCACCCA ATCCATGTCA AAATGGCGGC 
CAGTGTCAAG ACGGAGTCAA CTCTTACACC TGTACCTGTG CAGCTGGCTA CAGTGGAGAC 
GACTGTGAGA CCAATGACGA CGACTGTGCA CCCAATCCAT GTCAAAATGG CGGCCAATGT 
CAAGACGGAG TCAACTCTTA CACCTGTACC TGTGCAGCTG GCTACAGTGG AGACGACTGT 
GAGACCAATG ACGACGACTG TGCACCCAAT CCATGTCAAA ATGGCGGCCA GTGTCAAGAC 
GGAGTCAACT CTTACACCTG TTCCTGTGCA GCTGGCTACA GTGGAGACGA CTGTGAGACC 
AATGACGACG ACTGTGCACC CAATCCATGT CAAAATGGCG GCCAGTGTCA AGACGGAGTC 
AACTCTTACA CCTGTTCCTG TGCAGCTGGC TACAGTGGAG ACGACTGTGA GACCAATGAC 
GACGACTGTG CACCCAATCC ATGTCAAAAT GGCGGCCAGT GTCAAGACGG AGTCAACTCT 
TACACCTGCA ATTGTCCAGC TGGCTACAGT GGAGACGACT GTGAGACCAA TGACGACGAC 
TGTGCACCCA ATCCATGTCA AAATGGCGGC CAGTGTCAAG ACGGAGTCAA CTCTTACACC 
TGTAATTGTC CAGCTGGCTA CAGTGGAGAC GACTGTGAGA CCAATGACGA CGACTGTGCA 
CCCAATCCAT GTCAAAATGG CGGCCAGTGT CAAGACGGAG TCAACTCTTA CACCTGTAAC 
TGTCCAGCTG GCTACAGTGG AGACGACTGT GAGACCAATG ACGACGACTG TGCACCCAAT 
CCATGTCAAA ATGGCGGCCA GTGTCAAGAC GGAGTCAACT CTTACACCTG TAACTGTCCA 
GCTGGCTACA GTGGAGACGA CTGTGAGACC AATGACGACG ACTGTGCACC CAATCCATGT 
CAAAATGGCG GCCAGTGTCA AGACGGAGTC AACTCTTACA CCTGCAATTG TCCAGCTGGC 
TACAGTGGAG ACGACTGTGA GACCAATGAC GACGACTGTG CACCCAATCC ATGTCAAAAT 
GGCGGCCAGT GTCAAGACGG AGTCAACTCT TACACCTGTA CTTGTGCAGC TGGCTACAGT 
GGAGACGACT GTGAGATCAA TGACAACGAC TGTAATCCCA ATCCATGTCA AAATGGCGGC 
CAGTGTCAAG ACGGAGTCAA CTCTTACACC TGTGATTGTC CGGATGGCTT CAATGGATTC 
AACTGTCAGA CCAATATCAA CGAGTGCGCA TCTCGTCCAT GTCAAAATGG CGGCCAGTGT 
CAAGACGGTG TCAACTCCTA CACATGTGAT TGTCCTGATG GATATTTGGG AGAACACTGT 
GAAATTTCTG CGACAGACCC TCCACAGACG ACTCCTCAGG GGACAAGTAC CGTGTGTGAA 
TCTGGTTGGT TCCACCATGA GGGGAAGTGC TATTGTCTCC TTGATGACCA GTCCACCTAC 
AGCGAAGCTG CCCGGAGCTG CGCTGAGTTG GACGTCTACG GGCAGCTGGC CGTGGTGAAG 
CGGCCGAGCA CGCACCGCTT CCTGATGGAT TATGTCGAAC GGGAAGGTGG GGGTTCTCCG 
TGGATAGGAA TCAACGATGG CACTACAGAG GGTGTATGGA GGTACAGCAA TGGTGACCTT 
GTCAGTGATC CTTTCAACGA GTGGGCAGAT GGAACTTGGA ACAGCAGGAA GAAAGACTGT 
GTGCGCATGA GCGAAACCTT CGGGTACCAT TGGCAGCCTA TGCCGTGCGA CACGCAGCTG 
TCGTATATTT GCGAGTATCC TCTCCTGCCA CCCACAGATG CTCCAACCCC AACCACACCA 
GATGTTGAAC GTTGTGAGCC GGACACCTGT AACCAGGGAG GGATCTGTAT AGACGGACCG 
TCGTCCTACA CCTGTTACTG TCTGCCGGGG TTTACGGGAG ACCACTGTGA GACAGCTTGC 
CCTGCCGGAT TTACGCTGTT CCAGAAACAG TGCTACTGGT TCTCTCCAAC TTCCATGAGG 
GAAACCGTGG CAAATGCAGA AACAGACTGT GATGCCCGAG GTGCCCGTCT GGCTTGTGTG 
AAGGACGAAG ACACGCACAA CTTCCTCAAA TTGACCATCG CTACAACAAA CCGCAGGCCT 
TACTGGATCG GGTTGAATGA CACGGTGGTG GAGGATGTAT GGCGCCACAG TGACGGGACA 
CTTCTGGGCA GTTTCCGGCC TTTCCGCCCG TCCCGTAACA CCAACAATGT GTGGAGGGAC 
TGCGTTCTGA TGTGGCCAGC CTCTTCCTAC CAGTGGCTGT ACTATAACTG TAATTCTCGC 
AGGAACTACG TCTGCCAGAG GGCTGCAACT CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_101341 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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