BRAFLDRAFT_92999 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_92999
Entrez Gene ID	7245246
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002610229.1	XP_002610275.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_92999 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_92999 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb16923	XM_002610229.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_92999 ( XM_002610229.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002610275.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb16923
Clone ID Related Accession (Same CDS sequence)	XM_002610229.1
Accession Version	XM_002610229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101543). COMPLETENESS: incomplete on both ends.

ATGCAAAGAG ACGTCCACAA GATAATCATC TCAGCCTCAG AGCCTCAGGA GTTTGTACCC 
TACGGGCGTG AGTACACCTC CTGCCACCCG GATGGCTTTT CTCCACCCTC CCCCACCAGG 
ACGCTGGACG GCACGTCCAT GAGGGAGCAT GGTGTCCCCA TCAAGTCCTT CAGTGAGGTT 
TCCATGGCAG ACACACCCAA GAAGGAGTCA TGGTCCCTTC AGGCTGGGGG AGAGGGGGAG 
GTGTGTGATG AGGAGCTGTA CACCAGGGGA AACACTGTGG TGTGGAGCAG GGGAGGCCAG 
AGTGCCACCA GGATGGTCAT CAGGTCCTTC ACTGTTCCTT CACCAGTCCA AGAGGCCCAG 
CATACATGGT CTACTAAATA TGGGCTACTG TTTGAAAGGA GCATCTCAGA GGCAGAAATT 
ACCAGTCCAA AAAGGGGTTC TGAGGCCTTA CCCAAGCTGT TCAGCATGTG TCATCCACTG 
GACGAGCTGT GTCCGGTCGT CTGCAAGTAC GGAGGGCCAC TTGGCGTCGG AAAACTGTCA 
TATGTCAACG ACCCCATGCA GCATGTGGTG TTTACCAGCG CTGATCCCTC CCTCGTGATG 
ACCTATGACA CAGTTCTGGG TGTCCACTCT GTCTGGACAC TGAGGACGGC TAGGAATGAG 
GAAGTTGCTG GCCTATCCAG CACTCACTAC GGTGACACGT CCACCATTCC GAGCATGCCC 
AGTTCGCATC ATGTGACCAC GCCACTTGCG GGCCTGAGCT CCAGTACGCA GTCCCGCCTC 
AGTCTGAGCC AGTCTCCGAG CCTGTCGCCT GCGTACAGAA CCTACAGCCG ACCCAGCACA 
CCCGGGTTAT CGTCAAGGTC TCACTCACCG GCTGTTCTCA GCCACATGGC CACTCTCAGT 
CGCTGCCAGT CCCCAGCACC AAACAACAGC AGTTCCTCCA AATCACTCAG CTACTCCATC 
TCCTCCCCGT GGGTCTCACG ATCGCCCGCA AATATGGCGA GGACCCCAGC CTCCTCCTGC 
AGCTTTGCCT CACCCGGTCG CACCTTCGAG TCGTACCTGG AGATGCCGAC ACCCCTCACA 
CCTGAAGTCT GCATGGACCA TGTGTGGACA GAAACTATGA CAGCAAAAAG GGATGGTGTG 
CAGGGCCAGG CCTCCAAGGC GTTCTTGATG TCAGACGTTT GTGGACAGAA GTTTCTCTGT 
TATCTGATAG GAAAGAAACA CGAGCTCAGA TGTGTGAAGT TTGAGCCCAG CAATGACAAT 
AGACAGCTCA TCTTTGGAGC CATCACTAAA ATCAGTGCCA GGGATGCCAT AGCATTGCAG 
AGTCTGAACA TGATGATTGT GTTGGATCTA CACGGAAGTG TTGTGTTGTA CACTGGAATA 
AGTAAGGTTG GTAAGGTGTA CCTGGCCAGC CTCAGCAGCA GCCATGGCCT GTCCCTCTCC 
ACCTCCCTCC CTCGGGCAGG CACTCCGCTG GATAGTCCAG TTCCAGGCAC CTCCAGCCGG 
GCTCCCAGCG CCATGAACAT TCATGAGGAG GTAGTACTCC TGTCCCCAGT ACCAGAGTTA 
GGAAACTCCT CCAAGCTTCA GGGCTCCACC TTCAGCGATG ACTTCTCCTT CCACACTGGG 
ATACACATCA ACCATGTCAG GGACCCTGTG GCCAACAGGT TCACTCTGGA GTTGAATAAT 
GGGAAACTTT GCAGCACGTC TATTCCGGAG ATGGCAACAT CTCCTCTTGT GAAAGCCTGC 
CTGGCAGCTT TAAGGACACA GCTTCCCAAG GACATTGCAG TGCAGCTTTT TGTAAAGTGG 
TACACTACCC ACAATGCTCC TGGATCTCCC AGCCAGTCAG AATGGAGCCT GTTTGTGAGC 
TGTTTGCTGA GCATGATGGG ATACAACACA GGGCGACTGT CTCTCACCAA CCCCAACCTC 
AGGTCTGAGT TGAACAGCTC CATGTCGCCA GTTATCGCAG CCAAGAAGGC TCGTTCATCA 
GAGCAGGGCA GCAATGAGGA CTGGGATTAC CTCCTGAGCT CCACCTATCA CCACAGCCTG 
TCCCCACGTG TGCGCGACGC CCTGACCCTG AAGACGGAGG GCCCGCTCTG TATCCGTGAC 
GATGTAGAGA TGCAGGAGCC GGCCACGCCC GTTCTAGATC CATCCGACAG CCTGTTCCCC 
CACATGCCCC TGGTGCTGTC TGCTCTGCAT CTTGTGTATG AGGACCAGAA GCTACATGGT 
CTGTCTCACC CTGAGCTGGA GCAGATAGCA GCCATGCTGT GTCAGTTAGC AAGTGATTTG 
TGTCTTGATG CTTACATGGA CCACTACCGC AGGGACTTCC CCTGTGTGGG CATATATGCA 
GTCGCTCCCA GCAACATCAG TCAAATATGC TTCTCTGTCC CAGACACTGA ACAGGCAGCT 
GCAGAGGTCG AGGCATCCGT TCATCACAGC ACGCCACTGC AAAGCCCCAC AGCCACGCCT 
CCCTGGACCA GATCACCCCC CGCAGCATCG CCCATAGAAA GGGCGATCTC GCCGAGACAG 
CCCAGCAGAA GACACAAGAT GCCAAAGAAG GTCTGGACGC CTTCGGGTCC ATCAGGTGCC 
AAGGAGCAAC TCCAGTTCTC CTCTTCCTTC CCTAACAAGC CTCCCAGCAT CCTTCACTGG 
TTGTACCAGT GCATGCTGGG AAGGCCTGTG GAACCCTTTC CACATGTTGC TGGAGTCTGT 
GACAAGACCA AGAATGTCAT CATGCTTTAC TCTCTATACT TCCAACCCCT GATGAAGGAA 
GAGGCCAGAG GGTTCCGTGA GGAGGGGTTG TTTATCAAGA GACTGTCAGC TGCAGGACAC 
AGGGATGGTG GACCTCGTCA TAAAGGAGTC AGCCTGGACT TTAAGCAGAA TTACCGGCTG 
GGCGAGCGTG TGGTGTTGCA CATGACAGAG ATCGGGATGA CTGCAGAGGA TCTGGAGATG 
TTACCGTTCG GTGTCTCCCT CCCCCTCCGT GTGGCCATCC ACGAGTGTCG CTGCAGCATG 
CCCACAGACT TCCCCGCCGC AGCATACAAA CTCATTGGTA CAACAGAAGA TGACGATGGC 
ATGGACACCC TTGACCAAGA GCTGCTGAAG CTGAGGTTCC CAGATGACTT GCGGGTCCAG 
GAGGTGCGCA AACTGCTGCA GTCATCCAAA CCTGCCACGG TGCATGTGGT GCAGAAACCT 
GAAGTCAGTG ACCATGACTA CCTGGAGGAG CAGGAGATGA AGCTGCTGTC TGTGTGCCAG 
AGGACCATGG CACTGCCGGT GGGAAGAGGG ATGTTTACCC TGTGCACCTA CCACCCTGTA 
CTGACGGAGC CGCTACCCAT CCCCAAACTC AACCTGACTG GCAAGGCACC TGCACCCAGA 
ACCACCACCA TTGAGCTGTC CAGAATAGAG GTTCCTACCA ACATGAATAT GTGGCCGTCC 
TTCCACAACG GTGTCGCAGC CGGACTCAAG GTCGCTCCTG AGTCATCAAA GATTGAGTCT 
TCATGGATCG TCTACAACAA GCCGAAGAGT GCTGATCTGA CCAATGAGCA TGCAGGATTC 
CTGATGGCGC TGGGTCTGAA TGGTTACCTG TCGAACCTGA ACACTCTGAA CCTGCATGAC 
TACCTGAACA GGGGTCACGA ACTCACCAGT GTAGGGGTGC TGCTGGGGGT GGCAGCTGCA 
AAGAGAGGCA CAATGGACGC GGCCACCACC AGGATGCTGA GTATCCATGT GCCGGCACTG 
CTCATCCCCA CAACAACCGA GCTGGACATC GCACACAACG TACAGGTCGC CGCAGTGATG 
GGGGTGGGAC TGGTGTACCA GGGGACCGGA CACAGGCACA TGGCAGAGGT CCTGCTGCAG 
GAGATAGGTG GGTGGGGTTA CCATGGTGAT GGGGGTGGGG TTACCATGCT GATGGGGGTG 
ATGGGGGTGG GGCTGGTGTA CCAGGGGACT GGACACAGGC ACATGGCAGA GGTCCTGCTG 
CAGGAGATAG TTGGGTGGGG TTACCATGGT GATGGGGTGG GGCTGGTGTA CCAGGGGACC 
GGACACAGGC ACATGGCAGA GGTCCTGCTG CAGGAGATAG GTGGGTGGGG TTACCATGGT 
GATGGGGGTG GGGTTACCAT GCTGATGGGG GTGATGGGGG TGGGGCTGGT GTACCAGGGG 
ACTGGACACA GGCACATGGC AGAGGTCCTG CTGCAGGAGA TAGTTGGGTG GGGTTACCAT 
GGTGATGGGG TGGGGCTGGT GTACCAGGGG ACTGGACACA GGCACATGGC AGAGGTCCTG 
CTGCAGGAGA TAGGAAGGCC CCCTGGCCCT GAGATGGAGA ACTGCACAGA CAGGGAGTCC 
TATTCACTGG CAGCTGGGCT GGCTCTGGGC ATGGTGACAT TTGGTCTTGG AGGAGAGAGT 
GGGGGGCTGG CTGATCTAAA CATGGCTGAC CAGCTGTATC ACTTCATGGT GGGGGGACAC 
AAGAAACCGC TGGAGGGTGA CCAGGTGAAC ATTGATGTGA CGGCTTCAGG GGCAACCATA 
GCTCTTGGCA TGATGTTCTT CCGGACTAAC AACCAATCTG TTGCCCAGTG GCTGTCTCCT 
CCTGACACAC AGTTCCTCCT GGAGTTTGTG AGGCCAGACT TCCTCATGCT CAGGGTGATT 
GCCAAGGGAC TAGTGATGTG GGACAGCATC CAGCCCACTA AGGAGTGGAT CTGGGGAAAC 
ATACCCAAGA TTGTGGAGCA GTTTGCCTTC AAACAGAAGA AAGAAGAGGT GCAGGCACAA 
GCTACAGCCC TGGGGATAGA CTATCAGACA GTCAGTCAAG CCAAGGCCAA CATCGTGGCA 
GGAGCCTGTC TGGTCCTGGG CCTCAGATTT GCAGGCACAG CTAACCAGGA AGCTTACCAG 
ACTCTGTTCT CATTTGCCAA TGATGTCATC TTGCTGACAA GAAAGCAGTC ACTTGTTGAG 
CAGGCTGGCC GGCACATTGT GGAAGCCTGC CTCGTTGTGA TTGTTGTGTC ACTGTCGCTG 
GTGATGGCAG GGACAGGCGA CCTGGGCGTC CTGCGAATCT GTCGGCACCT GCACCACCGC 
ATGCTGCCGG AGATCACCTA CGGGATGCAC ATGGCCGTCC ACTCCGCACT GGGCCTGCTG 
TTCCTGGGAG GGGGAAGATA CTCCCTGAAC ACCAGCCCTG AGTCCATAGC TGTCCTGCTG 
TGTGCCCTGT TTCCCAAGTA CCCTGCACAC AGTAATGACA ACAGGTACCA TCTGCAGGCC 
CTCAGACACC TGTATGTTTT GGCTGCTGAG CCACGACTGT TGCTACCACG GGATGTGGAT 
ACCAACAAGG CCTGCTACAC AAAACTGCAG CTTATCTTTA AGGAAACCCA GTGGCACAAG 
GAGTGCAGTG TGGAGACCAT GGCTCCCAGC ATCCTGCCAG AGATCAGCAG ACTCAGACAG 
ATTTCCATTC TGGGCCCCAG ATACTGGCCG ATCACCATTG ACACCCAAAA GAATGCTGAC 
ATGCTCAGGG AACTTCTGAA GAACGGTGGT GTGGTGTATG TTAAACAAAG AGCTGGTCAC 
ATGTCCTTCT CAGACGATCC TAAGGGCTTC AGAAGTCTTC TTGCTCAGAG TTCCATTGGT 
GGGGAGTCAA GACACCACTC AGTTACGGCG GAGGAAGTGA AGTCCTTTTC GTCAGACCCT 
AATGTCGAGG CATTTGCTGA CTACTTCTGT ACAGTCAAGA CAGACACTGA AAAGGAGCAG 
GAGAAGCTGT CCTGTGTGGC CTCCTTGTTG TATGAGTGCA TCACCCAGGA GAAACCTGAT 
CTGGTGCCAA CACACCTGGC AGCCATGCAG GTGGTGGACA GTCTGCAGGT GTGTCCTAGC 
TCCTTACAGC TGGCCCAGGT GAAGCTGCTG CTGGCCTTCC ACAAGGCAGC CCAGCAACAG 
GGGCAGGACC GGGGCAGGCC GTTGCTAAGC GATGAGTTCT TACTCAGCCT GAGGAGCAAG 
ATTGACAGTT GCCTGGACCA ATGGCTGAAA AGGTCTGGCC ATGCTGTTGG GAGGTACATC 
CGTGAAGGGG TCATCGCCCC TGACGACGGT CTGCTGTCCT GTTTCCTGGT GTTTTATAGC 
ATTCCTCCTC ATCACCAGCT GGTACAGTCA GGCATCTCCA TGGCAACAGA TGGCTCCCTC 
AGTATTCCTG AGCTGGTGCT GAGACTGAAG GAACTGAGTC TTCCCACCTC AAGTCTCATC 
AAGATGCTGT CTGCTCTGTC TTTAAGTCAC CCAACAGAGA CTCCTTCAAA CAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002610275.1
CDS	1..6237
Translation	MQRDVHKIIISASEPQEFVPYGREYTSCHPDGFSPPSPTRTLDGTSMREHGVPIKSFSEVSMADTPKKESWSLQAGGEGEVCDEELYTRGNTVVWSRGGQSATRMVIRSFTVPSPVQEAQHTWSTKYGLLFERSISEAEITSPKRGSEALPKLFSMCHPLDELCPVVCKYGGPLGVGKLSYVNDPMQHVVFTSADPSLVMTYDTVLGVHSVWTLRTARNEEVAGLSSTHYGDTSTIPSMPSSHHVTTPLAGLSSSTQSRLSLSQSPSLSPAYRTYSRPSTPGLSSRSHSPAVLSHMATLSRCQSPAPNNSSSSKSLSYSISSPWVSRSPANMARTPASSCSFASPGRTFESYLEMPTPLTPEVCMDHVWTETMTAKRDGVQGQASKAFLMSDVCGQKFLCYLIGKKHELRCVKFEPSNDNRQLIFGAITKISARDAIALQSLNMMIVLDLHGSVVLYTGISKVGKVYLASLSSSHGLSLSTSLPRAGTPLDSPVPGTSSRAPSAMNIHEEVVLLSPVPELGNSSKLQGSTFSDDFSFHTGIHINHVRDPVANRFTLELNNGKLCSTSIPEMATSPLVKACLAALRTQLPKDIAVQLFVKWYTTHNAPGSPSQSEWSLFVSCLLSMMGYNTGRLSLTNPNLRSELNSSMSPVIAAKKARSSEQGSNEDWDYLLSSTYHHSLSPRVRDALTLKTEGPLCIRDDVEMQEPATPVLDPSDSLFPHMPLVLSALHLVYEDQKLHGLSHPELEQIAAMLCQLASDLCLDAYMDHYRRDFPCVGIYAVAPSNISQICFSVPDTEQAAAEVEASVHHSTPLQSPTATPPWTRSPPAASPIERAISPRQPSRRHKMPKKVWTPSGPSGAKEQLQFSSSFPNKPPSILHWLYQCMLGRPVEPFPHVAGVCDKTKNVIMLYSLYFQPLMKEEARGFREEGLFIKRLSAAGHRDGGPRHKGVSLDFKQNYRLGERVVLHMTEIGMTAEDLEMLPFGVSLPLRVAIHECRCSMPTDFPAAAYKLIGTTEDDDGMDTLDQELLKLRFPDDLRVQEVRKLLQSSKPATVHVVQKPEVSDHDYLEEQEMKLLSVCQRTMALPVGRGMFTLCTYHPVLTEPLPIPKLNLTGKAPAPRTTTIELSRIEVPTNMNMWPSFHNGVAAGLKVAPESSKIESSWIVYNKPKSADLTNEHAGFLMALGLNGYLSNLNTLNLHDYLNRGHELTSVGVLLGVAAAKRGTMDAATTRMLSIHVPALLIPTTTELDIAHNVQVAAVMGVGLVYQGTGHRHMAEVLLQEIGGWGYHGDGGGVTMLMGVMGVGLVYQGTGHRHMAEVLLQEIVGWGYHGDGVGLVYQGTGHRHMAEVLLQEIGGWGYHGDGGGVTMLMGVMGVGLVYQGTGHRHMAEVLLQEIVGWGYHGDGVGLVYQGTGHRHMAEVLLQEIGRPPGPEMENCTDRESYSLAAGLALGMVTFGLGGESGGLADLNMADQLYHFMVGGHKKPLEGDQVNIDVTASGATIALGMMFFRTNNQSVAQWLSPPDTQFLLEFVRPDFLMLRVIAKGLVMWDSIQPTKEWIWGNIPKIVEQFAFKQKKEEVQAQATALGIDYQTVSQAKANIVAGACLVLGLRFAGTANQEAYQTLFSFANDVILLTRKQSLVEQAGRHIVEACLVVIVVSLSLVMAGTGDLGVLRICRHLHHRMLPEITYGMHMAVHSALGLLFLGGGRYSLNTSPESIAVLLCALFPKYPAHSNDNRYHLQALRHLYVLAAEPRLLLPRDVDTNKACYTKLQLIFKETQWHKECSVETMAPSILPEISRLRQISILGPRYWPITIDTQKNADMLRELLKNGGVVYVKQRAGHMSFSDDPKGFRSLLAQSSIGGESRHHSVTAEEVKSFSSDPNVEAFADYFCTVKTDTEKEQEKLSCVASLLYECITQEKPDLVPTHLAAMQVVDSLQVCPSSLQLAQVKLLLAFHKAAQQQGQDRGRPLLSDEFLLSLRSKIDSCLDQWLKRSGHAVGRYIREGVIAPDDGLLSCFLVFYSIPPHHQLVQSGISMATDGSLSIPELVLRLKELSLPTSSLIKMLSALSLSHPTETPSNR

Target ORF information:

RefSeq Version	XM_002610229.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002610229.1

ORF Insert Sequence:

ATGCAAAGAG ACGTCCACAA GATAATCATC TCAGCCTCAG AGCCTCAGGA GTTTGTACCC 
TACGGGCGTG AGTACACCTC CTGCCACCCG GATGGCTTTT CTCCACCCTC CCCCACCAGG 
ACGCTGGACG GCACGTCCAT GAGGGAGCAT GGTGTCCCCA TCAAGTCCTT CAGTGAGGTT 
TCCATGGCAG ACACACCCAA GAAGGAGTCA TGGTCCCTTC AGGCTGGGGG AGAGGGGGAG 
GTGTGTGATG AGGAGCTGTA CACCAGGGGA AACACTGTGG TGTGGAGCAG GGGAGGCCAG 
AGTGCCACCA GGATGGTCAT CAGGTCCTTC ACTGTTCCTT CACCAGTCCA AGAGGCCCAG 
CATACATGGT CTACTAAATA TGGGCTACTG TTTGAAAGGA GCATCTCAGA GGCAGAAATT 
ACCAGTCCAA AAAGGGGTTC TGAGGCCTTA CCCAAGCTGT TCAGCATGTG TCATCCACTG 
GACGAGCTGT GTCCGGTCGT CTGCAAGTAC GGAGGGCCAC TTGGCGTCGG AAAACTGTCA 
TATGTCAACG ACCCCATGCA GCATGTGGTG TTTACCAGCG CTGATCCCTC CCTCGTGATG 
ACCTATGACA CAGTTCTGGG TGTCCACTCT GTCTGGACAC TGAGGACGGC TAGGAATGAG 
GAAGTTGCTG GCCTATCCAG CACTCACTAC GGTGACACGT CCACCATTCC GAGCATGCCC 
AGTTCGCATC ATGTGACCAC GCCACTTGCG GGCCTGAGCT CCAGTACGCA GTCCCGCCTC 
AGTCTGAGCC AGTCTCCGAG CCTGTCGCCT GCGTACAGAA CCTACAGCCG ACCCAGCACA 
CCCGGGTTAT CGTCAAGGTC TCACTCACCG GCTGTTCTCA GCCACATGGC CACTCTCAGT 
CGCTGCCAGT CCCCAGCACC AAACAACAGC AGTTCCTCCA AATCACTCAG CTACTCCATC 
TCCTCCCCGT GGGTCTCACG ATCGCCCGCA AATATGGCGA GGACCCCAGC CTCCTCCTGC 
AGCTTTGCCT CACCCGGTCG CACCTTCGAG TCGTACCTGG AGATGCCGAC ACCCCTCACA 
CCTGAAGTCT GCATGGACCA TGTGTGGACA GAAACTATGA CAGCAAAAAG GGATGGTGTG 
CAGGGCCAGG CCTCCAAGGC GTTCTTGATG TCAGACGTTT GTGGACAGAA GTTTCTCTGT 
TATCTGATAG GAAAGAAACA CGAGCTCAGA TGTGTGAAGT TTGAGCCCAG CAATGACAAT 
AGACAGCTCA TCTTTGGAGC CATCACTAAA ATCAGTGCCA GGGATGCCAT AGCATTGCAG 
AGTCTGAACA TGATGATTGT GTTGGATCTA CACGGAAGTG TTGTGTTGTA CACTGGAATA 
AGTAAGGTTG GTAAGGTGTA CCTGGCCAGC CTCAGCAGCA GCCATGGCCT GTCCCTCTCC 
ACCTCCCTCC CTCGGGCAGG CACTCCGCTG GATAGTCCAG TTCCAGGCAC CTCCAGCCGG 
GCTCCCAGCG CCATGAACAT TCATGAGGAG GTAGTACTCC TGTCCCCAGT ACCAGAGTTA 
GGAAACTCCT CCAAGCTTCA GGGCTCCACC TTCAGCGATG ACTTCTCCTT CCACACTGGG 
ATACACATCA ACCATGTCAG GGACCCTGTG GCCAACAGGT TCACTCTGGA GTTGAATAAT 
GGGAAACTTT GCAGCACGTC TATTCCGGAG ATGGCAACAT CTCCTCTTGT GAAAGCCTGC 
CTGGCAGCTT TAAGGACACA GCTTCCCAAG GACATTGCAG TGCAGCTTTT TGTAAAGTGG 
TACACTACCC ACAATGCTCC TGGATCTCCC AGCCAGTCAG AATGGAGCCT GTTTGTGAGC 
TGTTTGCTGA GCATGATGGG ATACAACACA GGGCGACTGT CTCTCACCAA CCCCAACCTC 
AGGTCTGAGT TGAACAGCTC CATGTCGCCA GTTATCGCAG CCAAGAAGGC TCGTTCATCA 
GAGCAGGGCA GCAATGAGGA CTGGGATTAC CTCCTGAGCT CCACCTATCA CCACAGCCTG 
TCCCCACGTG TGCGCGACGC CCTGACCCTG AAGACGGAGG GCCCGCTCTG TATCCGTGAC 
GATGTAGAGA TGCAGGAGCC GGCCACGCCC GTTCTAGATC CATCCGACAG CCTGTTCCCC 
CACATGCCCC TGGTGCTGTC TGCTCTGCAT CTTGTGTATG AGGACCAGAA GCTACATGGT 
CTGTCTCACC CTGAGCTGGA GCAGATAGCA GCCATGCTGT GTCAGTTAGC AAGTGATTTG 
TGTCTTGATG CTTACATGGA CCACTACCGC AGGGACTTCC CCTGTGTGGG CATATATGCA 
GTCGCTCCCA GCAACATCAG TCAAATATGC TTCTCTGTCC CAGACACTGA ACAGGCAGCT 
GCAGAGGTCG AGGCATCCGT TCATCACAGC ACGCCACTGC AAAGCCCCAC AGCCACGCCT 
CCCTGGACCA GATCACCCCC CGCAGCATCG CCCATAGAAA GGGCGATCTC GCCGAGACAG 
CCCAGCAGAA GACACAAGAT GCCAAAGAAG GTCTGGACGC CTTCGGGTCC ATCAGGTGCC 
AAGGAGCAAC TCCAGTTCTC CTCTTCCTTC CCTAACAAGC CTCCCAGCAT CCTTCACTGG 
TTGTACCAGT GCATGCTGGG AAGGCCTGTG GAACCCTTTC CACATGTTGC TGGAGTCTGT 
GACAAGACCA AGAATGTCAT CATGCTTTAC TCTCTATACT TCCAACCCCT GATGAAGGAA 
GAGGCCAGAG GGTTCCGTGA GGAGGGGTTG TTTATCAAGA GACTGTCAGC TGCAGGACAC 
AGGGATGGTG GACCTCGTCA TAAAGGAGTC AGCCTGGACT TTAAGCAGAA TTACCGGCTG 
GGCGAGCGTG TGGTGTTGCA CATGACAGAG ATCGGGATGA CTGCAGAGGA TCTGGAGATG 
TTACCGTTCG GTGTCTCCCT CCCCCTCCGT GTGGCCATCC ACGAGTGTCG CTGCAGCATG 
CCCACAGACT TCCCCGCCGC AGCATACAAA CTCATTGGTA CAACAGAAGA TGACGATGGC 
ATGGACACCC TTGACCAAGA GCTGCTGAAG CTGAGGTTCC CAGATGACTT GCGGGTCCAG 
GAGGTGCGCA AACTGCTGCA GTCATCCAAA CCTGCCACGG TGCATGTGGT GCAGAAACCT 
GAAGTCAGTG ACCATGACTA CCTGGAGGAG CAGGAGATGA AGCTGCTGTC TGTGTGCCAG 
AGGACCATGG CACTGCCGGT GGGAAGAGGG ATGTTTACCC TGTGCACCTA CCACCCTGTA 
CTGACGGAGC CGCTACCCAT CCCCAAACTC AACCTGACTG GCAAGGCACC TGCACCCAGA 
ACCACCACCA TTGAGCTGTC CAGAATAGAG GTTCCTACCA ACATGAATAT GTGGCCGTCC 
TTCCACAACG GTGTCGCAGC CGGACTCAAG GTCGCTCCTG AGTCATCAAA GATTGAGTCT 
TCATGGATCG TCTACAACAA GCCGAAGAGT GCTGATCTGA CCAATGAGCA TGCAGGATTC 
CTGATGGCGC TGGGTCTGAA TGGTTACCTG TCGAACCTGA ACACTCTGAA CCTGCATGAC 
TACCTGAACA GGGGTCACGA ACTCACCAGT GTAGGGGTGC TGCTGGGGGT GGCAGCTGCA 
AAGAGAGGCA CAATGGACGC GGCCACCACC AGGATGCTGA GTATCCATGT GCCGGCACTG 
CTCATCCCCA CAACAACCGA GCTGGACATC GCACACAACG TACAGGTCGC CGCAGTGATG 
GGGGTGGGAC TGGTGTACCA GGGGACCGGA CACAGGCACA TGGCAGAGGT CCTGCTGCAG 
GAGATAGGTG GGTGGGGTTA CCATGGTGAT GGGGGTGGGG TTACCATGCT GATGGGGGTG 
ATGGGGGTGG GGCTGGTGTA CCAGGGGACT GGACACAGGC ACATGGCAGA GGTCCTGCTG 
CAGGAGATAG TTGGGTGGGG TTACCATGGT GATGGGGTGG GGCTGGTGTA CCAGGGGACC 
GGACACAGGC ACATGGCAGA GGTCCTGCTG CAGGAGATAG GTGGGTGGGG TTACCATGGT 
GATGGGGGTG GGGTTACCAT GCTGATGGGG GTGATGGGGG TGGGGCTGGT GTACCAGGGG 
ACTGGACACA GGCACATGGC AGAGGTCCTG CTGCAGGAGA TAGTTGGGTG GGGTTACCAT 
GGTGATGGGG TGGGGCTGGT GTACCAGGGG ACTGGACACA GGCACATGGC AGAGGTCCTG 
CTGCAGGAGA TAGGAAGGCC CCCTGGCCCT GAGATGGAGA ACTGCACAGA CAGGGAGTCC 
TATTCACTGG CAGCTGGGCT GGCTCTGGGC ATGGTGACAT TTGGTCTTGG AGGAGAGAGT 
GGGGGGCTGG CTGATCTAAA CATGGCTGAC CAGCTGTATC ACTTCATGGT GGGGGGACAC 
AAGAAACCGC TGGAGGGTGA CCAGGTGAAC ATTGATGTGA CGGCTTCAGG GGCAACCATA 
GCTCTTGGCA TGATGTTCTT CCGGACTAAC AACCAATCTG TTGCCCAGTG GCTGTCTCCT 
CCTGACACAC AGTTCCTCCT GGAGTTTGTG AGGCCAGACT TCCTCATGCT CAGGGTGATT 
GCCAAGGGAC TAGTGATGTG GGACAGCATC CAGCCCACTA AGGAGTGGAT CTGGGGAAAC 
ATACCCAAGA TTGTGGAGCA GTTTGCCTTC AAACAGAAGA AAGAAGAGGT GCAGGCACAA 
GCTACAGCCC TGGGGATAGA CTATCAGACA GTCAGTCAAG CCAAGGCCAA CATCGTGGCA 
GGAGCCTGTC TGGTCCTGGG CCTCAGATTT GCAGGCACAG CTAACCAGGA AGCTTACCAG 
ACTCTGTTCT CATTTGCCAA TGATGTCATC TTGCTGACAA GAAAGCAGTC ACTTGTTGAG 
CAGGCTGGCC GGCACATTGT GGAAGCCTGC CTCGTTGTGA TTGTTGTGTC ACTGTCGCTG 
GTGATGGCAG GGACAGGCGA CCTGGGCGTC CTGCGAATCT GTCGGCACCT GCACCACCGC 
ATGCTGCCGG AGATCACCTA CGGGATGCAC ATGGCCGTCC ACTCCGCACT GGGCCTGCTG 
TTCCTGGGAG GGGGAAGATA CTCCCTGAAC ACCAGCCCTG AGTCCATAGC TGTCCTGCTG 
TGTGCCCTGT TTCCCAAGTA CCCTGCACAC AGTAATGACA ACAGGTACCA TCTGCAGGCC 
CTCAGACACC TGTATGTTTT GGCTGCTGAG CCACGACTGT TGCTACCACG GGATGTGGAT 
ACCAACAAGG CCTGCTACAC AAAACTGCAG CTTATCTTTA AGGAAACCCA GTGGCACAAG 
GAGTGCAGTG TGGAGACCAT GGCTCCCAGC ATCCTGCCAG AGATCAGCAG ACTCAGACAG 
ATTTCCATTC TGGGCCCCAG ATACTGGCCG ATCACCATTG ACACCCAAAA GAATGCTGAC 
ATGCTCAGGG AACTTCTGAA GAACGGTGGT GTGGTGTATG TTAAACAAAG AGCTGGTCAC 
ATGTCCTTCT CAGACGATCC TAAGGGCTTC AGAAGTCTTC TTGCTCAGAG TTCCATTGGT 
GGGGAGTCAA GACACCACTC AGTTACGGCG GAGGAAGTGA AGTCCTTTTC GTCAGACCCT 
AATGTCGAGG CATTTGCTGA CTACTTCTGT ACAGTCAAGA CAGACACTGA AAAGGAGCAG 
GAGAAGCTGT CCTGTGTGGC CTCCTTGTTG TATGAGTGCA TCACCCAGGA GAAACCTGAT 
CTGGTGCCAA CACACCTGGC AGCCATGCAG GTGGTGGACA GTCTGCAGGT GTGTCCTAGC 
TCCTTACAGC TGGCCCAGGT GAAGCTGCTG CTGGCCTTCC ACAAGGCAGC CCAGCAACAG 
GGGCAGGACC GGGGCAGGCC GTTGCTAAGC GATGAGTTCT TACTCAGCCT GAGGAGCAAG 
ATTGACAGTT GCCTGGACCA ATGGCTGAAA AGGTCTGGCC ATGCTGTTGG GAGGTACATC 
CGTGAAGGGG TCATCGCCCC TGACGACGGT CTGCTGTCCT GTTTCCTGGT GTTTTATAGC 
ATTCCTCCTC ATCACCAGCT GGTACAGTCA GGCATCTCCA TGGCAACAGA TGGCTCCCTC 
AGTATTCCTG AGCTGGTGCT GAGACTGAAG GAACTGAGTC TTCCCACCTC AAGTCTCATC 
AAGATGCTGT CTGCTCTGTC TTTAAGTCAC CCAACAGAGA CTCCTTCAAA CAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_92999 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

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