BRAFLDRAFT_71907 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_71907
Entrez Gene ID	7233198
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002613437.1	XP_002613483.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_71907 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_71907 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb12519	XM_002613437.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_71907 ( XM_002613437.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002613483.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb12519
Clone ID Related Accession (Same CDS sequence)	XM_002613437.1
Accession Version	XM_002613437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101565). COMPLETENESS: incomplete on both ends.

ATGCCGTGGT ATTTCCCGTG GTCGGACTCC ATCAAGAAGC GGGCGGTGCG CTATTTACTG 
CAGCACTACT TGGGCCACTT CCTGCAGGAG AAACTGACCT TGGATCAACT CAGTGTTGAT 
CTGTATAACG GCAAAGGCAT CATCAAAAAT GTGCCCCTGG ATGTTTGGTC CCTGAATGAG 
GTGCTAGAGG GCACTAATGC CCCAGTGGAG ATGATTGATG GCTACATCGG CAGCATCTCA 
GTTGCAGTTC CCTGGTCGGC CCTCCTCAAT GACAACTGTA CCATGGAGAT CAAGGGCCTG 
GAAGTGACTC TGCAACCAAA GTATCGGCCG GAGAACTCCA GCAGTATGCC AGGGAGCATG 
ATGAACTCCA TCTGGCAGGG CAGCATGACC ACCAGCATGC AGCTGGCTCA GGAGTGTCTC 
AAACAGGAGG CGGCAGCAGG GGACGAGCAG GAGGTCCAGC CGTTCGAGGG GCTGGAGCTC 
TTTGCACAGA CTATCGAGAC AGTTCTGACC AGAATAAAAG TGACTTTTGT GGACACCATA 
GTAAGGATTG AACATGTGCC TAAAGACTCC AAGACTGGAA CTGCTCTGGA AATCCACATC 
AAAAGAATTG AGTACTTTGA TGAGTGTGCA GCAGACCAGG GCAGTCCTGT AGACAAGGAT 
GAGCCCAAGC TGATGCAGCC GGCAGCCGTG GCTACCAAGG TGTTCCACAT GATGGGGGTC 
ACCCTCCACT CTGACGAGTT CCCCGAGAGT GCCAGGACCA GTGCAAGATG TTCACCTAAC 
ATGTCTCCTC AGTCATCAGA CTCCAACCTC TCCCCACCCC TGACACCCAA GATGCCGTCC 
ATGATGGAGG GGAGGGGGGC AGAGTCGGAC CCCCACCCAC AGGTCCCCTC CAACCCTATC 
AAGCTGGGCG TCTGCTCGGG CATGCAGGAG ATCAAGGTCA AGGTCAAGCA GAGTGAAAGT 
ATATCAGGAC CCAAGTTTGA CCTGGAGTGC TACCTGGGGT CGTTGAACTT CTTCCTGAGC 
CCCAGGCAGG TTCACCTGCT GATGGAGATG GCCAACGAGA TCCTCACACC AGCCTCGACC 
AGTATGAGTC CTGATGGTCG CCTCAACAAG CCCATCCGCC CTGAAGATTA CCAGAAGATC 
GAGAGCGAAC TCCAGCGCCA GTTGTACGAG GATCGCCAGC CCAAACAGGC GGCGGCAGGG 
GGTTGGGAGG ATCACATTGG GGACTACCAC CTGGCCATGG GCAGGCACGC CATGGAAGGG 
TCACAGAAGA AAGGACTAGG TGATGATGAT GATGACGTGT ACTACTCCAT GGCAGCAGGG 
CTGAACGTAC AGCTGCCCCC CCTCGGACAG CCCCCTGAGG TGGACCTGGA GACATCCTTC 
GGTAGTAACT ACAGCGCCAG CAGCAGTGAC TCAGCTAGAC ACAGACTGCC CAGAAACAAC 
AGCGCCCACA AGTTTGGATA CAGCAGCTAC GCAGGAGGAG CCTCCTCCCC CTTCACAGCC 
ACACTTCCTA AAGGGGTCAC CACCAACCAG AAACCCTCCA GCAGAAAAAG AAGCCAGGAG 
TTGCTGGAAG AGGAGGCCAA GGCGGAGCGG ACCCACTACA GACTGAAGCT GAGCTGCCTG 
TCAATCACTC TCCTGCACAA CGACCCTGTG GCCACGCCCA CCAGTAAGAG TGACAGGCCA 
GAGTCCAGTA ATCTGCTGTT AGACATGGCG GAGAAGTACT TCACAGACGT GATGGGCTAC 
AGTACGTCTG CTTTTGGGTG GAAGGACCTG GCTGCCATGA AGGACAGAAT AGCCAAGGCC 
TGCCAGTATG ACCATCTGCA ATGTGTAGCT GCCCCTGTGA TGCTGGAGTG TGATCAGAAG 
TCCAACAGCA GTGCTCACCA CCACTTCCAG CGGACAGTAG CGGACATCAG TGTGGGTCTG 
CTGGACCTGG TGGAGTGTCT GTACGACAGG GACTCTCGGT CTGACATGAC CTCCGACCCC 
ACAGAACCTA CATACACAGA GCTGTTGTCC TTCCCTGACC TTCAGCAGCA GGAGACCACC 
AAGTCCATGT TTGCTGCTCT CCCCACCACC AGTCCCTGCC TTAAAGTCAA GTTCAAGAAT 
GTAGACAAAG GAGCAAAGGG TGCATACACA AAGCCCCCCA TCCCCGAGGT GACAGTAGAA 
GTCAGTACCT GTGAATGTGA AGTGGACATC AGTATAGTGG ACAGACTGAA TGCCCTGCTG 
AACCCCCAGC CCCTGGCACA GATGAGCAGC TCCTCCGGCA TGTTCAGCTC CATCCAGCCC 
ACAGCCAGTA TGACAAGACA GGCCTTGTTC AGTGAAGCCT TAGACGAGAC ACCAGCCGCA 
TGTGAAAACC CCATCAGCCT GCATATATTA TCCCCAGCTG CCAAGTTTCA CGTCAGATTC 
CCCATCCCCG ACCTGAGACC TCCCAACCTG GACCACCTAC CCTGGTGGAA GAAACACATC 
AGGAAGGAGG TACTCATCCT GGATGTGACG GACTTTGACT TCCAGACAAC CCTGGGGATG 
GGCGACATCT CACAGTACGA GCTCAAGTTC AGAGAAGTGC ATGGCCTGTA TCAGCTAGAC 
CCGTCAGAGG AAGCAGTGTC TTTTTGTAGG ATCTCCCATG GCCAGTCGGA GGGTGACATT 
GATGCTCCTG TCAGCAGTGG ATTTGATTGG CCAAGGATAG TTGTGACTGT AAAGCCATAC 
AATGCTGGGT CAGTACTGGA GGAGGAGGAA GTGGAGTCTG AACCCAGCAC ACCTAACCAC 
TCGCTGGAGG AAACACTCAC CGGCAAGCAG GAGCCATCCG TCTTCTCCAA CAAGAAAGTC 
ATGTTTGAGA ATGAAGAGAT GATCATGCCG GGTGACCAGG CAGAGTTGTC AGACTTCCAG 
GAGACAACAG TCGCCACAGC TAGGATATGT CTGGAGTTCT CCTTCCCCAA CGTCAACCTG 
GTGTTGCCAG ACAAAGCCTT TTATGAGAAC CTGTACAATA GAATAAACAA TGACCTGTGT 
CTGTGGGCGC CGGCTGCCCC ATCACCGGTC AACCCGAGTG ACCAGTACAG CCTGCAGGGC 
CAGGTGGGGC TGGACCTGGC CAGTCAGTTC ATGCAGGGGG CCAGCAAGGA CAGGTTCATC 
ATGTGCAAGT CTGCCCTGCA GTATGATTCT GATACTGATG ATGAAGATGA GTTTGACAGC 
GGCCCATTTG ACAGTGGACC TTTCTACTCT ACATCTGAGA TGAAACAGAG ACAGCAGAGG 
AAACAGAAGC AGGAGCAGTA CAGACAGAGG AGAGCACAGA GCTTTGTGGC TGTCTCTGTC 
AACATTGGGC ATGGCAAACT GGCCCTGGGC ACAAGGATAA AGGATGAGGA GGGCAAGTCA 
TCTGAAAACA GACAGGGGGA GCTGCTACTG GAAGTCAAGG ATGGGACTAT CTTTGTGGTG 
GTGAACTACC AAGGGAACCC TAACCTGGGC TACCTGTTTG TGCAAGCCAA CAAAGCTGGG 
GTTTTCCACA GAGGTCAGGT AGATGACCAC ACATGGGAGG ACATAGTGGG CCGAGCCACA 
CCGGACTGCC CTGACCAGCT GGACCCGCTG ATCTACCGTT CTGACAGCGG TGTGTACACC 
AGTCTGGCCG GGCTGGTAGG CACAGGAGGG GACAGTCTCA ACATGCTGTC TGTGGGAGTC 
AGGATCACAT TAGACACAGA GAAGGCTGAA ACTGTCAAGG AGTTCTTGGT TGCTGTTGCT 
GTGCGAGGAG GAACACTAAG ACACAAGGTG TTTCTGCAAG GACAGCACTG GTTTACACAG 
ATGATAGATT TCCTGGATGT AGAAGACTAC CCTATCTTGG GCTACACCTT ACCACACATC 
CTGACAGAGC TGCATGTCCA CCTGCTGGGC TGTGCTGTGG ACTACAGGCC TGTCCACCTG 
CCAATGAGGG CTATGCTGAC CATGGAGAAG CTCAGCATAT CCAGCAATAT TGTTGTAGAT 
TCTGCTATTT CTGTTTTAAG ATTTATTGTA GATGACTCTG CATTGTATCT TTCTGCTAAT 
GTGAGGGACC AAGGACCAGT AGACCTAAAG AGAAACTATG TTTGTGTGAT GGACATGGGG 
CTGTTTGAAC TCAAGCTGAC CTTAAATGAA GGAACAGACT CAAAACAGCC CAGAGTAGAC 
CTGAAGGCCT CCAACAACAT GCTGCACATC CGGACCTGCA GTGACTCGCT GGCTGCCCTG 
ACGGCACTGC TGCAGTACTT AGCTGAGGAT GGGGACCTCA GGGTGGGGGA GATGGAGTCT 
AGTGAGGAGA TCAGGAGAAA GAGACAGCCG TCCCAGTCCA GACCCCGCTC AGACAGCCCC 
ATCCCACAGG TGCTGGACCA TGTACAGGAC CAGATGGAGG AGGCCATGAT GGACGTGTGG 
GATGTCAATG GTGAAGCTGG TCTGGACCCC AGCATGACCC TGCAGTCCAG TCCTCTGTCA 
GACAAGAGAA CTCTTTCCTC CTCTCTTCAC GCATCCTCTG CCGCTGTGCC ATATCAGACC 
ACTCCGGCCC AGTCTACAGG TCCCGGCAGT GAGCCCTCTA GCCTGACCCA GTCCCCACCC 
TCCGCCATGT TCTTGTTCCC AGATGAGAAT GGCCAGTCCC GCGCCCCCCT CCCACCTTCA 
TCTGTGGCGA CTGAGGATGA CTCGGGCAGT GCTGCTGACA GTGTTGAGGA CTGGAGCGAT 
GACAGGGATG AGGATTTCTG TATCATTGAT GACCCTGGAC TTGGTATCAT GCCCAGACAT 
GGTGAGCCGG AGGTCCGTGT GCTGACAGAA GACCCCATCA GGGTGGAGGA TAACCACTTC 
AGCCAGCCCC TGGGGAAGTC AGACCTGCTG AAGGCGCCCG ACCACTTCCC CCCGGCCGTG 
CTGAGGTATG TGAGAAGTCC TCAGCACTCT CCAGCTAAGG CAGGTTCCCA GCGGGACAGC 
TACAGCAGAC ACGGGCGGCT GGGCTGGCAG GCCAGGGGGG GTCCGGGCAG GGACACGGAC 
GTGCATGTGG AGCTGCAGAT GAACAAGGTG CGATTACAAC ACGAGGTGTT CCCCGAGGAT 
ACAGAGCAGG CGTCGCGCCA GGTGCTGATC ATCACGGAGC TGGAGATCAG GGACAGGCTG 
GCCTCCTCAC AGATCAACAA GTTCCTGTAT CAGTACACCT CCGAGGCCAG GCCCAAACAG 
GCGCACGCCA ACATGGTGAT GATCAAGGCC CTGCACATCC GGCCGGACCC CCATCTGAAG 
GCCCAGGAGT GCTGCCTCAG GGTGTCCCTC CTACCTCTCA GACTCAACAT AGATCAGGAT 
GCCATCAACT TCCTGAGAGA CTTTTTTGCC GAGCTTGCTG ATGAAAGTGG TGCCTGCCCT 
GATGCAGTAG ACAGCAGTGC CAGCCCCCTG CAGGCCTCCC CTCCCAGCCC GGTCATGCTG 
GTGGGGTCTC CCCTGTACAG TGATGTGGGG GGTGAGGACA CAGTCAGGGG GGCAGAGGGT 
ACAGACTCCA GCATGACGTC CTCCAGGGGC AGTGGGGCGT CAGAGTCCAG TCCTGCTGGA 
CCAATCTTCT TCAGGGAGTT CACCTTTTCT CCTGAGGTGC CCATCAGACT AGACTACCAT 
GGGAAGAGGG TGGACATGGA ACAGGGTGCA TTTGCAGGTT TGCTGCTAGG ACTCAGTCAA 
CTCAACAACT CTGAGTTGAA GCTGAGAAGA CTCAACTATA GACATGGGCT GCTGGGGTAT 
GACAAGATGG TGGCCTATGC TGTGACAGAG TGGGGGGAGG ACATCAGGAA GAACCAGCTT 
CCTGGGATCC TGGGTGGTGT AGGACCTATG CATTCTCTAG TACAAATTGT CCAAGGTATA 
GTGGACCTAG TGTGGCTGCC TATTGAACAG TACCGTAAAG ATGGTCGCAT CGTCCGAGGC 
CTGCAGAGAG GAGCGAGCTC CTTTGCCACG TCCACCGCCA TGGCGACCCT GGAGCTGACT 
AACCGCGTGG TGCAGTCCAT CCAGACTGCG GCAGAGTTCA CGTATGACAT GTTGTCATCA 
GGACCCAGCG TCAGGAAGCT GGGGCAGGGG GCCTTCATCC AGCACCGCAT GGCACAACAG 
CCTGCCGACG TGCGAGAGGG CGTTACTAAG GCTTATAATG TGGTCAGGGA GGGTGTTACG 
GACACAGCCC ATACCATCTA CCGTGTGGCG GCAGAGCAGC ACGAACAGAA GGGTGTGACG 
GGGGCGGTGG GAGGTGTGCT GCGGCAGATC CCCCCCACCA TGGTCAAACC TGTCATCCTG 
GCCACAGAGG CCACCTCTAA TGTCATCGGT GGTGTCCGCA ACCAGTTCCT GCCAGACGCA 
CGACGCGAGG CTTCAGAAAA GTGGCGGTCA GAGGAGCAAA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002613483.1
CDS	1..6345
Translation	MPWYFPWSDSIKKRAVRYLLQHYLGHFLQEKLTLDQLSVDLYNGKGIIKNVPLDVWSLNEVLEGTNAPVEMIDGYIGSISVAVPWSALLNDNCTMEIKGLEVTLQPKYRPENSSSMPGSMMNSIWQGSMTTSMQLAQECLKQEAAAGDEQEVQPFEGLELFAQTIETVLTRIKVTFVDTIVRIEHVPKDSKTGTALEIHIKRIEYFDECAADQGSPVDKDEPKLMQPAAVATKVFHMMGVTLHSDEFPESARTSARCSPNMSPQSSDSNLSPPLTPKMPSMMEGRGAESDPHPQVPSNPIKLGVCSGMQEIKVKVKQSESISGPKFDLECYLGSLNFFLSPRQVHLLMEMANEILTPASTSMSPDGRLNKPIRPEDYQKIESELQRQLYEDRQPKQAAAGGWEDHIGDYHLAMGRHAMEGSQKKGLGDDDDDVYYSMAAGLNVQLPPLGQPPEVDLETSFGSNYSASSSDSARHRLPRNNSAHKFGYSSYAGGASSPFTATLPKGVTTNQKPSSRKRSQELLEEEAKAERTHYRLKLSCLSITLLHNDPVATPTSKSDRPESSNLLLDMAEKYFTDVMGYSTSAFGWKDLAAMKDRIAKACQYDHLQCVAAPVMLECDQKSNSSAHHHFQRTVADISVGLLDLVECLYDRDSRSDMTSDPTEPTYTELLSFPDLQQQETTKSMFAALPTTSPCLKVKFKNVDKGAKGAYTKPPIPEVTVEVSTCECEVDISIVDRLNALLNPQPLAQMSSSSGMFSSIQPTASMTRQALFSEALDETPAACENPISLHILSPAAKFHVRFPIPDLRPPNLDHLPWWKKHIRKEVLILDVTDFDFQTTLGMGDISQYELKFREVHGLYQLDPSEEAVSFCRISHGQSEGDIDAPVSSGFDWPRIVVTVKPYNAGSVLEEEEVESEPSTPNHSLEETLTGKQEPSVFSNKKVMFENEEMIMPGDQAELSDFQETTVATARICLEFSFPNVNLVLPDKAFYENLYNRINNDLCLWAPAAPSPVNPSDQYSLQGQVGLDLASQFMQGASKDRFIMCKSALQYDSDTDDEDEFDSGPFDSGPFYSTSEMKQRQQRKQKQEQYRQRRAQSFVAVSVNIGHGKLALGTRIKDEEGKSSENRQGELLLEVKDGTIFVVVNYQGNPNLGYLFVQANKAGVFHRGQVDDHTWEDIVGRATPDCPDQLDPLIYRSDSGVYTSLAGLVGTGGDSLNMLSVGVRITLDTEKAETVKEFLVAVAVRGGTLRHKVFLQGQHWFTQMIDFLDVEDYPILGYTLPHILTELHVHLLGCAVDYRPVHLPMRAMLTMEKLSISSNIVVDSAISVLRFIVDDSALYLSANVRDQGPVDLKRNYVCVMDMGLFELKLTLNEGTDSKQPRVDLKASNNMLHIRTCSDSLAALTALLQYLAEDGDLRVGEMESSEEIRRKRQPSQSRPRSDSPIPQVLDHVQDQMEEAMMDVWDVNGEAGLDPSMTLQSSPLSDKRTLSSSLHASSAAVPYQTTPAQSTGPGSEPSSLTQSPPSAMFLFPDENGQSRAPLPPSSVATEDDSGSAADSVEDWSDDRDEDFCIIDDPGLGIMPRHGEPEVRVLTEDPIRVEDNHFSQPLGKSDLLKAPDHFPPAVLRYVRSPQHSPAKAGSQRDSYSRHGRLGWQARGGPGRDTDVHVELQMNKVRLQHEVFPEDTEQASRQVLIITELEIRDRLASSQINKFLYQYTSEARPKQAHANMVMIKALHIRPDPHLKAQECCLRVSLLPLRLNIDQDAINFLRDFFAELADESGACPDAVDSSASPLQASPPSPVMLVGSPLYSDVGGEDTVRGAEGTDSSMTSSRGSGASESSPAGPIFFREFTFSPEVPIRLDYHGKRVDMEQGAFAGLLLGLSQLNNSELKLRRLNYRHGLLGYDKMVAYAVTEWGEDIRKNQLPGILGGVGPMHSLVQIVQGIVDLVWLPIEQYRKDGRIVRGLQRGASSFATSTAMATLELTNRVVQSIQTAAEFTYDMLSSGPSVRKLGQGAFIQHRMAQQPADVREGVTKAYNVVREGVTDTAHTIYRVAAEQHEQKGVTGAVGGVLRQIPPTMVKPVILATEATSNVIGGVRNQFLPDARREASEKWRSEEQK

Target ORF information:

RefSeq Version	XM_002613437.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002613437.1

ORF Insert Sequence:

ATGCCGTGGT ATTTCCCGTG GTCGGACTCC ATCAAGAAGC GGGCGGTGCG CTATTTACTG 
CAGCACTACT TGGGCCACTT CCTGCAGGAG AAACTGACCT TGGATCAACT CAGTGTTGAT 
CTGTATAACG GCAAAGGCAT CATCAAAAAT GTGCCCCTGG ATGTTTGGTC CCTGAATGAG 
GTGCTAGAGG GCACTAATGC CCCAGTGGAG ATGATTGATG GCTACATCGG CAGCATCTCA 
GTTGCAGTTC CCTGGTCGGC CCTCCTCAAT GACAACTGTA CCATGGAGAT CAAGGGCCTG 
GAAGTGACTC TGCAACCAAA GTATCGGCCG GAGAACTCCA GCAGTATGCC AGGGAGCATG 
ATGAACTCCA TCTGGCAGGG CAGCATGACC ACCAGCATGC AGCTGGCTCA GGAGTGTCTC 
AAACAGGAGG CGGCAGCAGG GGACGAGCAG GAGGTCCAGC CGTTCGAGGG GCTGGAGCTC 
TTTGCACAGA CTATCGAGAC AGTTCTGACC AGAATAAAAG TGACTTTTGT GGACACCATA 
GTAAGGATTG AACATGTGCC TAAAGACTCC AAGACTGGAA CTGCTCTGGA AATCCACATC 
AAAAGAATTG AGTACTTTGA TGAGTGTGCA GCAGACCAGG GCAGTCCTGT AGACAAGGAT 
GAGCCCAAGC TGATGCAGCC GGCAGCCGTG GCTACCAAGG TGTTCCACAT GATGGGGGTC 
ACCCTCCACT CTGACGAGTT CCCCGAGAGT GCCAGGACCA GTGCAAGATG TTCACCTAAC 
ATGTCTCCTC AGTCATCAGA CTCCAACCTC TCCCCACCCC TGACACCCAA GATGCCGTCC 
ATGATGGAGG GGAGGGGGGC AGAGTCGGAC CCCCACCCAC AGGTCCCCTC CAACCCTATC 
AAGCTGGGCG TCTGCTCGGG CATGCAGGAG ATCAAGGTCA AGGTCAAGCA GAGTGAAAGT 
ATATCAGGAC CCAAGTTTGA CCTGGAGTGC TACCTGGGGT CGTTGAACTT CTTCCTGAGC 
CCCAGGCAGG TTCACCTGCT GATGGAGATG GCCAACGAGA TCCTCACACC AGCCTCGACC 
AGTATGAGTC CTGATGGTCG CCTCAACAAG CCCATCCGCC CTGAAGATTA CCAGAAGATC 
GAGAGCGAAC TCCAGCGCCA GTTGTACGAG GATCGCCAGC CCAAACAGGC GGCGGCAGGG 
GGTTGGGAGG ATCACATTGG GGACTACCAC CTGGCCATGG GCAGGCACGC CATGGAAGGG 
TCACAGAAGA AAGGACTAGG TGATGATGAT GATGACGTGT ACTACTCCAT GGCAGCAGGG 
CTGAACGTAC AGCTGCCCCC CCTCGGACAG CCCCCTGAGG TGGACCTGGA GACATCCTTC 
GGTAGTAACT ACAGCGCCAG CAGCAGTGAC TCAGCTAGAC ACAGACTGCC CAGAAACAAC 
AGCGCCCACA AGTTTGGATA CAGCAGCTAC GCAGGAGGAG CCTCCTCCCC CTTCACAGCC 
ACACTTCCTA AAGGGGTCAC CACCAACCAG AAACCCTCCA GCAGAAAAAG AAGCCAGGAG 
TTGCTGGAAG AGGAGGCCAA GGCGGAGCGG ACCCACTACA GACTGAAGCT GAGCTGCCTG 
TCAATCACTC TCCTGCACAA CGACCCTGTG GCCACGCCCA CCAGTAAGAG TGACAGGCCA 
GAGTCCAGTA ATCTGCTGTT AGACATGGCG GAGAAGTACT TCACAGACGT GATGGGCTAC 
AGTACGTCTG CTTTTGGGTG GAAGGACCTG GCTGCCATGA AGGACAGAAT AGCCAAGGCC 
TGCCAGTATG ACCATCTGCA ATGTGTAGCT GCCCCTGTGA TGCTGGAGTG TGATCAGAAG 
TCCAACAGCA GTGCTCACCA CCACTTCCAG CGGACAGTAG CGGACATCAG TGTGGGTCTG 
CTGGACCTGG TGGAGTGTCT GTACGACAGG GACTCTCGGT CTGACATGAC CTCCGACCCC 
ACAGAACCTA CATACACAGA GCTGTTGTCC TTCCCTGACC TTCAGCAGCA GGAGACCACC 
AAGTCCATGT TTGCTGCTCT CCCCACCACC AGTCCCTGCC TTAAAGTCAA GTTCAAGAAT 
GTAGACAAAG GAGCAAAGGG TGCATACACA AAGCCCCCCA TCCCCGAGGT GACAGTAGAA 
GTCAGTACCT GTGAATGTGA AGTGGACATC AGTATAGTGG ACAGACTGAA TGCCCTGCTG 
AACCCCCAGC CCCTGGCACA GATGAGCAGC TCCTCCGGCA TGTTCAGCTC CATCCAGCCC 
ACAGCCAGTA TGACAAGACA GGCCTTGTTC AGTGAAGCCT TAGACGAGAC ACCAGCCGCA 
TGTGAAAACC CCATCAGCCT GCATATATTA TCCCCAGCTG CCAAGTTTCA CGTCAGATTC 
CCCATCCCCG ACCTGAGACC TCCCAACCTG GACCACCTAC CCTGGTGGAA GAAACACATC 
AGGAAGGAGG TACTCATCCT GGATGTGACG GACTTTGACT TCCAGACAAC CCTGGGGATG 
GGCGACATCT CACAGTACGA GCTCAAGTTC AGAGAAGTGC ATGGCCTGTA TCAGCTAGAC 
CCGTCAGAGG AAGCAGTGTC TTTTTGTAGG ATCTCCCATG GCCAGTCGGA GGGTGACATT 
GATGCTCCTG TCAGCAGTGG ATTTGATTGG CCAAGGATAG TTGTGACTGT AAAGCCATAC 
AATGCTGGGT CAGTACTGGA GGAGGAGGAA GTGGAGTCTG AACCCAGCAC ACCTAACCAC 
TCGCTGGAGG AAACACTCAC CGGCAAGCAG GAGCCATCCG TCTTCTCCAA CAAGAAAGTC 
ATGTTTGAGA ATGAAGAGAT GATCATGCCG GGTGACCAGG CAGAGTTGTC AGACTTCCAG 
GAGACAACAG TCGCCACAGC TAGGATATGT CTGGAGTTCT CCTTCCCCAA CGTCAACCTG 
GTGTTGCCAG ACAAAGCCTT TTATGAGAAC CTGTACAATA GAATAAACAA TGACCTGTGT 
CTGTGGGCGC CGGCTGCCCC ATCACCGGTC AACCCGAGTG ACCAGTACAG CCTGCAGGGC 
CAGGTGGGGC TGGACCTGGC CAGTCAGTTC ATGCAGGGGG CCAGCAAGGA CAGGTTCATC 
ATGTGCAAGT CTGCCCTGCA GTATGATTCT GATACTGATG ATGAAGATGA GTTTGACAGC 
GGCCCATTTG ACAGTGGACC TTTCTACTCT ACATCTGAGA TGAAACAGAG ACAGCAGAGG 
AAACAGAAGC AGGAGCAGTA CAGACAGAGG AGAGCACAGA GCTTTGTGGC TGTCTCTGTC 
AACATTGGGC ATGGCAAACT GGCCCTGGGC ACAAGGATAA AGGATGAGGA GGGCAAGTCA 
TCTGAAAACA GACAGGGGGA GCTGCTACTG GAAGTCAAGG ATGGGACTAT CTTTGTGGTG 
GTGAACTACC AAGGGAACCC TAACCTGGGC TACCTGTTTG TGCAAGCCAA CAAAGCTGGG 
GTTTTCCACA GAGGTCAGGT AGATGACCAC ACATGGGAGG ACATAGTGGG CCGAGCCACA 
CCGGACTGCC CTGACCAGCT GGACCCGCTG ATCTACCGTT CTGACAGCGG TGTGTACACC 
AGTCTGGCCG GGCTGGTAGG CACAGGAGGG GACAGTCTCA ACATGCTGTC TGTGGGAGTC 
AGGATCACAT TAGACACAGA GAAGGCTGAA ACTGTCAAGG AGTTCTTGGT TGCTGTTGCT 
GTGCGAGGAG GAACACTAAG ACACAAGGTG TTTCTGCAAG GACAGCACTG GTTTACACAG 
ATGATAGATT TCCTGGATGT AGAAGACTAC CCTATCTTGG GCTACACCTT ACCACACATC 
CTGACAGAGC TGCATGTCCA CCTGCTGGGC TGTGCTGTGG ACTACAGGCC TGTCCACCTG 
CCAATGAGGG CTATGCTGAC CATGGAGAAG CTCAGCATAT CCAGCAATAT TGTTGTAGAT 
TCTGCTATTT CTGTTTTAAG ATTTATTGTA GATGACTCTG CATTGTATCT TTCTGCTAAT 
GTGAGGGACC AAGGACCAGT AGACCTAAAG AGAAACTATG TTTGTGTGAT GGACATGGGG 
CTGTTTGAAC TCAAGCTGAC CTTAAATGAA GGAACAGACT CAAAACAGCC CAGAGTAGAC 
CTGAAGGCCT CCAACAACAT GCTGCACATC CGGACCTGCA GTGACTCGCT GGCTGCCCTG 
ACGGCACTGC TGCAGTACTT AGCTGAGGAT GGGGACCTCA GGGTGGGGGA GATGGAGTCT 
AGTGAGGAGA TCAGGAGAAA GAGACAGCCG TCCCAGTCCA GACCCCGCTC AGACAGCCCC 
ATCCCACAGG TGCTGGACCA TGTACAGGAC CAGATGGAGG AGGCCATGAT GGACGTGTGG 
GATGTCAATG GTGAAGCTGG TCTGGACCCC AGCATGACCC TGCAGTCCAG TCCTCTGTCA 
GACAAGAGAA CTCTTTCCTC CTCTCTTCAC GCATCCTCTG CCGCTGTGCC ATATCAGACC 
ACTCCGGCCC AGTCTACAGG TCCCGGCAGT GAGCCCTCTA GCCTGACCCA GTCCCCACCC 
TCCGCCATGT TCTTGTTCCC AGATGAGAAT GGCCAGTCCC GCGCCCCCCT CCCACCTTCA 
TCTGTGGCGA CTGAGGATGA CTCGGGCAGT GCTGCTGACA GTGTTGAGGA CTGGAGCGAT 
GACAGGGATG AGGATTTCTG TATCATTGAT GACCCTGGAC TTGGTATCAT GCCCAGACAT 
GGTGAGCCGG AGGTCCGTGT GCTGACAGAA GACCCCATCA GGGTGGAGGA TAACCACTTC 
AGCCAGCCCC TGGGGAAGTC AGACCTGCTG AAGGCGCCCG ACCACTTCCC CCCGGCCGTG 
CTGAGGTATG TGAGAAGTCC TCAGCACTCT CCAGCTAAGG CAGGTTCCCA GCGGGACAGC 
TACAGCAGAC ACGGGCGGCT GGGCTGGCAG GCCAGGGGGG GTCCGGGCAG GGACACGGAC 
GTGCATGTGG AGCTGCAGAT GAACAAGGTG CGATTACAAC ACGAGGTGTT CCCCGAGGAT 
ACAGAGCAGG CGTCGCGCCA GGTGCTGATC ATCACGGAGC TGGAGATCAG GGACAGGCTG 
GCCTCCTCAC AGATCAACAA GTTCCTGTAT CAGTACACCT CCGAGGCCAG GCCCAAACAG 
GCGCACGCCA ACATGGTGAT GATCAAGGCC CTGCACATCC GGCCGGACCC CCATCTGAAG 
GCCCAGGAGT GCTGCCTCAG GGTGTCCCTC CTACCTCTCA GACTCAACAT AGATCAGGAT 
GCCATCAACT TCCTGAGAGA CTTTTTTGCC GAGCTTGCTG ATGAAAGTGG TGCCTGCCCT 
GATGCAGTAG ACAGCAGTGC CAGCCCCCTG CAGGCCTCCC CTCCCAGCCC GGTCATGCTG 
GTGGGGTCTC CCCTGTACAG TGATGTGGGG GGTGAGGACA CAGTCAGGGG GGCAGAGGGT 
ACAGACTCCA GCATGACGTC CTCCAGGGGC AGTGGGGCGT CAGAGTCCAG TCCTGCTGGA 
CCAATCTTCT TCAGGGAGTT CACCTTTTCT CCTGAGGTGC CCATCAGACT AGACTACCAT 
GGGAAGAGGG TGGACATGGA ACAGGGTGCA TTTGCAGGTT TGCTGCTAGG ACTCAGTCAA 
CTCAACAACT CTGAGTTGAA GCTGAGAAGA CTCAACTATA GACATGGGCT GCTGGGGTAT 
GACAAGATGG TGGCCTATGC TGTGACAGAG TGGGGGGAGG ACATCAGGAA GAACCAGCTT 
CCTGGGATCC TGGGTGGTGT AGGACCTATG CATTCTCTAG TACAAATTGT CCAAGGTATA 
GTGGACCTAG TGTGGCTGCC TATTGAACAG TACCGTAAAG ATGGTCGCAT CGTCCGAGGC 
CTGCAGAGAG GAGCGAGCTC CTTTGCCACG TCCACCGCCA TGGCGACCCT GGAGCTGACT 
AACCGCGTGG TGCAGTCCAT CCAGACTGCG GCAGAGTTCA CGTATGACAT GTTGTCATCA 
GGACCCAGCG TCAGGAAGCT GGGGCAGGGG GCCTTCATCC AGCACCGCAT GGCACAACAG 
CCTGCCGACG TGCGAGAGGG CGTTACTAAG GCTTATAATG TGGTCAGGGA GGGTGTTACG 
GACACAGCCC ATACCATCTA CCGTGTGGCG GCAGAGCAGC ACGAACAGAA GGGTGTGACG 
GGGGCGGTGG GAGGTGTGCT GCGGCAGATC CCCCCCACCA TGGTCAAACC TGTCATCCTG 
GCCACAGAGG CCACCTCTAA TGTCATCGGT GGTGTCCGCA ACCAGTTCCT GCCAGACGCA 
CGACGCGAGG CTTCAGAAAA GTGGCGGTCA GAGGAGCAAA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_71907 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

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