BRAFLDRAFT_107340 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_107340
Entrez Gene ID	7227043
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002598854.1	XP_002598900.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_107340 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_107340 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb10081	XM_002598854.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_107340 ( XM_002598854.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002598900.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb10081
Clone ID Related Accession (Same CDS sequence)	XM_002598854.1
Accession Version	XM_002598854.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101458). COMPLETENESS: incomplete on both ends.

ATGTACGAGC AAGCGCTAGC GGAGCAAGCG GGGCAAGTTC AGTCGCCCCT CTACGGCCAC 
GGCCAAACCA GCGGGCCGCC GACCCAACCC CCTCCTGTCC ACCAGCCCAA AGGCGTTTCT 
GACCAGGAGC AAGAAGACCA AGGAACTACT CCAAACACGT ACGCAGAAGC AGAAATAGTG 
TACTACACAA TCAAGGATCA AGATCTGCCG CAGTCTTTAC GTGGGATGGG GCTGAAGCAG 
GAGTCACCCC AGGGGGAGGT GGATACCAGC GGGCGATCGC CCCACCAGGG TGGTTCCCGC 
GGGCGTGTCC ACCATGGCAA CGGCGCTTCT GACGAGTCGC AAGAAGAGCA AGCGGGGCAA 
GTTCAGTCGC CCCTCTACGG CCACGGCCAA ACCAGCGAGC CGCCGACCCA ACCCCCTCCT 
GTCCACCAGC CCAAAGGCGT TTCTGACCAG GAGCAAGAAG ACCAAGGAAC TTCTCCAAAC 
ACGTACGCAG AAGCAGAAAA AGTGTACTAC ACAATCAAGG ATCAAGATCT GCCGCCGTCT 
TTACGTGGGG TGGGGCTGAA GCAGGAGTCA CCCCAGGAGG AGATGGAAAC CAGCGGGCCA 
CCGCCCCACC AGGGTGGTTC CCGCGGGCGT GTCCGCCATG GCAACGGCGC TTCTGACGAG 
TCGCAAGAAG AGCAAGAAGC GTGTACAAAC GTCTACGAAG AAGTCGAGGA TATGAAGCGT 
TGCGCAACAT GCACTTCTGA AGACGACCTA CGCGAACTGT CCACCACGGT TGAAGCCTTG 
AAGCGCGACC AAGACGACCT ATGGCAACTG TCCACCACTG TTGACGCCTT GAAGCAGGTT 
GAAGACATGT GCCTACTGTG CGCGGATCAC AGTTCCGAGA CGATTAAGAC GACAGGTGAT 
AACAACAGTG ACCCATTCCT CTTGGTCGCT GACTGGAAAG GGCAAACCAT TCTGCAAGTT 
GACATCATTT CCGGATCAAA GGTCACACTT CCGCTTGAGG GTGTGGACCA GCCCTACGCC 
CTGGACTACG ACCCGCTCAC CGACTACGTG TACTGGTCAG ATGGAGGGAA CAAGAAAATC 
AAACGGGCTC GTCGTGATGG AAGTGGCATG GAGACCATCA TAGATACCGG TAACAATCAT 
ACTATCAATA GCCTTGGCCT GGCCCTGGAC CACGCTGGAG GGAATATCTA CTGGTCTGCC 
GTCGGCGCAA AGACTATCTC TGTAGCAAAG AAAGATGGAT CGTCCGCTAG GACACTTCTG 
ACGTCACCAG ACATCGGGTA CCCTAGAGAC CTGGTCCTGG ACCCCAGGAA TGATGATCAG 
GAATACGGCA TCTACAGCTC AGACCTGCTG GGCAACGATA TCCGAGAGGT GCTGTATGAA 
CCCGGGAAGT ATGTGGAGGG AATCGCGGTT GATGAGGACT TCGTCTACTG GTCGTCTGAG 
TGGTTTGATT CATCATCATC ATCATCCTTT CAAGGAAAAA TCGGAAAACT GTCCAAATCC 
GACTTGACCA AGACTGTGCT GGTGGATGGC CTGAAGTACC CAGTGGGCAT CTACCTGTCC 
ACGGCAGCGC CTCCTGGCGT AATCAATGGC CACGTTGACG TCATCAGACA TGACAGAACA 
ACCAACAGCG ATCCCAACAT CAGTCCATAC GCTGTTACAC ACGAGCTGAC CATGCCTTAT 
AAAGGTAAAG CTGCAACCCA GAGCGGACAG GGAAATGACA CCATTCCACC AAACAGCGAT 
ACATACATGT GCCATGCCCT TGCAATGAAG ACACTTTCGG AAGATACCAA GACTGTTCAA 
CCAAACAGCG ATACATACAT GTGCCATGCC CTTGCAATGA AGACACTTTC GGAAGATACC 
AAGACTGTTC AACCGAACAG CGATACATAC ATGTGCCATG CCCTTGCGAT GAATACACTT 
TCGGAAGATA CAAAGACCAA GGCGCCCATT CAGAAAAAAA TAGTGGCTTC AACCAGCAAA 
GAAGATTTCC CAGTTCCGTT AGCAGGACGT GATAGCCCCG AGAACAGTGC TGATGCTCCA 
GCCCTTTCCG GGAATGGCGG TACCATGCGC GAGTCTCCAC ATCCTCCAAA CGAAGCCTCC 
GTGCACATGA CCCCCCTATC TAACTTCACG CACGAGCACA ACCCGCGGCC CTCTGACCCC 
GAGCTTGCAC GTGACGCCTC TAACCCGGTG TACGGGCATG ACCTGGACGC ATCCAACCCG 
GTGTACGGAC AGGGCCAGAA TACGGATCCT GATACTGACT TGCGACCGGC TGGCATGCAG 
CCATATGCCG TCAGATATCA GGAGGAAGAT GCTGATGATG ATAAAGACCA TCAACCAGCT 
AACATGGAGC CATATGCCGT CAGATATCAG GAGGAAGATG CTGATGATGA TAAAGACCAT 
CAACCAGCTA ACATGGAGCC ATATGCCGTC AGATATCAGG AGGAAGATGC TGATGATGAT 
AAAGACCATC AACCGGCTGG CATGGAGCCA TATGCCGTCA GATATCAGGA CGAGGATGCT 
GATGATGATA AAGACCATCA ACCGGCTGGC ATGGAGCCAT ATGCCGGCAG ATATCAGGAC 
GAGGATGCTG ATGATGATAA TGACCATCGA CCGGCTAGCA TGGAGCCATA TGTCGATCCG 
GGAATGACGC TGTCACTTCA GGGAAAGATA CTGGCACTTC AGTTCCACGA AAGGATGACA 
GGAACATGCG CCGCACTCGA AAACACACAG GGTGTTCCCC ACGGTCCTCG GGAAGCAGCT 
GGCGGCACCA CACCCAAGCA GCAGACCGAT TTTCAGGCAC GTGCGGACGC CGCTGCGAGT 
GTACCCAACC CTATGTACGG GGTGCGGTGG GTGATGTACG CCTCCAGAGC AGGCACCACA 
CCCAAGCAGC AGACTGATTG GCAGGCACGT GCGGACGCCG CTGCGAGTAT ACCCAACCCT 
ATGTACGCCT CCAGAGCAGA CCGCCTGTAT CCGGGTGGAG CAAGCGGCTG CCGTGCTCTC 
TGTAGCTTCA TCCGCTCGCA GCTTACCTAC ATCTCCACGG GCATTGCCGT GCTGCTAGGC 
CTGGTCGCTG TGGGACTTGT CATTTTCATT TTCATCAACA ATGAGGAGAT ATCTGAACAG 
ACCACCACTG TTGACGTTCA GACCACCACT GTTGACGCCT TAAAGCGCGA CCAAGACAAC 
ATGTCCACCA CTGTTGATGC CTTGAAGCGC GACCAAGACA ACATGTCCAC CACTGTTGAT 
GCCCTGAAGC GCGACCAAGA CGACATGCAC CAACTGTCCA CCACTGTTGA CGCCTTGAAG 
CGCGACCAAG ACGACATGCG CCAACTGTCC ACCACTGTTG ACGCCTTGAA GAGCGACCAA 
GACGACATGC GCCAACTGTC CACCACTGTT GACGCCTTAA AACGCGACCT GGACAAGGAG 
CGCAGCCGAA TCGCCACCTC GGAGCAACGT CTTGACGCGA CGATTAAGAC GCCAAGAGAC 
CATGACTCCC GTAAGAACAG TGAGCCATTC CTCTTGGTCT CTTGTCCTGA TGAAGAAACC 
ATCATCCAAG TTGACATCAC TTCCGGATCA AAGATCACAC TGCCACTGGT AGCTGTTGGA 
CAGCCCTACG CCCTGGACTA CGACCCGCTC ACCGACTACG TGTACTGGTC AGATTATTCA 
AATTATTGGA ACGGCATCAT CAAACGGGCT CGTCGTGATG GAAGTGGCAT GGAGACCATC 
ATAGATGTAA AGGGATTCCA TGCCTATGGC CTGGCACTGG ACCACGCTGG GGGAAATATC 
TACTGGTCTG TCGCTGTCCC AGAGACTATC TCTGTAGCAA AGATGGATGG ATCGTCCGCT 
AGAACACTTC TGACGTCACC AGCCATCAAT ATGCCTTACG AACTGGTCCT GGACCCCAGG 
AATGGACTCA TGTACTGGGT GGACGGTTAT AACAAAGGCA GGATTTGCCG AGCTGCGATG 
GACGGCAGTA ACCAAACCAC CATCATCACG GGTCTGAATA AGCCCCAAGC CATTACAATA 
GACTTCAATG AGGATCGGCT GTACTACCGT GATCAGGAGT ACGGCATCTA CAGCTCAGAC 
CTACAGGGCA ACGATATCCG AGAAGTGGTG CATGAAGACG GGAAGTGGGT TTTGGGAATC 
GCGGTTGATG AGGACTTCGT CTACTGGTCG TCCGGCTGGC TGTCCAAGTC CGACCTGACC 
AAGACTGTGC TGGTGGATGG CCTGGAGACT CAAGGCATCT ACCTGTCCAC GGCAGCGCCT 
CCTGGCGTAA CCAATGTACA TATTTGTGCC TCCAATTCTG AGGCAGTTAT TTTCATTAAC 
AAAATTGCCT CGGGTAGGAA AGAAGCGGTT ACGTTTGGAG TGACATCTTC AGGAAGGAGT 
AACGTCTTGA GTCGCATCTT CAGGAAGCGG CAACGTCTTG TCCTAGCAGA CTCCTACACG 
GCCATCCACG GGCGTTGGCT GGAGGGAGGA AGGGAGGGAG GGAGGATAGC AAGGGAGGGA 
ACAACCAACA GCGATCCCAA CATCAGTCCA TACGCTGTTA CACACGAGCT GACCATGCCT 
TATAAAGGTA AAGCTGCAAC CCAGAGCGGA CAGGGAAATG ACACCATTCC ACCAAACAGC 
GATACATACA TGTGCCATGC CCTTGCAATG AAGACACTTT CGGAAGATAC CAAGACTGTT 
CAACCAAACA GCGATACATA CATGTGCCAT GCCCTTGCAA TGAAGACACT TTCGGAAGAT 
ACCAAGACTG TTCAACCGAA CAGCGATACA TACATGTGCC ATGCCCTTGC GATGAATACA 
CTTTCGGAAG ATACAAAGAC CAAGGCGCCC ATTCAGAAAA AAATAGTGGC TTCAACCAGC 
AAAGAAGATT TCCCAGTTCC GTTAGCAGGA CGTGATAGCC CCGAGAACAG TGCTGATGCT 
CCAGCCCTTT CCGGGAATGG CGGTACCATG CGCGAGTCTC CACATCCTCC AAACGAAGCC 
TCCGTGCACA TGACCCCCCT ATCTAACCTC ACGCACGAGC ACAACCCGCG GCCCTCTGAC 
CCCGAGCTTG CACGTGACGC CTCCAACCCG GTGTACGGGC ATGACCTGGA CGCATCCAAC 
CCGGTGTACG GACAGGGCCA GGGTACGGAT CCTGATACTG ACTTGCGACC GGCTAGCATG 
GAGCCATATG CCGTCAGATA TCTGGAGGAA GATGCTGATG ATGATAAAGA CCATCGACCA 
GCTGGCATGG AGCCATATGC CGAGATATCT GAACAGACCA CCACTGTTGA CGTTCAGACC 
ACCACTGTTG ACGCCTTAAA GCGCGACCAA GACAACATGT CCACCACTGT TGATGCCTTG 
AAGCGCGACC AAGACAACAT GTCCACCACT GTTGATGCCC TGAAGCGCGA CCAAGACGAC 
ATGCACCAAC TGTCCACCAC TGTTGACGCC TTGAAGCGCG ACCAAGACGA CATGCGTCAA 
CTGTCCACCA CTGTTGACGC CTTGAAGAGC GACCAAGACG ACATGCGCCA ACTGTCCACC 
ACTGTTGACG CCTTGAAGAG CGACCAAGAC GACATGCGCC AACTGCCCAC CACTGTTGAC 
GCCTTAAAAC GCGACCTGGA CAAGGAGCGC AGCCGAATCG CCACCTCGGA GCAGCGTCTT 
GACGCGACGA TTAAGACGCC AAGAGACCAT GACTCCCGTA AGAACAGTGA GCCATTCCTC 
TTGGTCGCTG ATCGTAATAA AGAAACCATC ATCCAAGTTG ACATCACTTC CGGGTCAAAG 
CTCACACTAC CACTGGTAGG TGTTGGATGG CCCTACGTCC TGGACTTCGA CCCGCTCACC 
GACTTCGTGT ACTGGTCAGA TGGATGGAAC ATCAACATCA AACGGGCTCG TCGTGATGGA 
AGTGGCATGG AGACCATCAC AGATGTAAAG AGAGATGCCT ATGGCCTGGC ATTGGACCAC 
GCTGGGGGGA ATATCTACTG GTCTGTCTAT TACGCAAAGA CTATCTCTGT AGCAAAGAAG 
GATGGATCGT CCGCTAGGAC ACTTCTGACG TCACCAGCCA TCAATCACCC TTACGGCCTG 
GTCCTGGACC CCAGGAATGG ACTCATGTAC TGGGTGGACG TTTCTAACAA AGGCAGGATT 
TGCCGAGCTG CGATGGACGG CAGTAACCAA ACCACCATCA TCACGGGTCT GAATCGGCCC 
AGCGCCATCA CAATAGACTT CACTGAGGAT CGGCTGTACT ACAGTGATCA GTATTACATC 
TACAGCTCAG ACCTGCTGGG CAACGATATC CGACAGGTGC TGTATGAAGA CGGGAAGTGG 
GTTCATGGCA TCGCGGTTGA TGAGGACTTC GTCTACTGGT CGTCCAGGTG GTGGTATTCA 
TCATCATCAT CATCCTCTTG GGGAAAAATC GGAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002598900.1
CDS	1..6399
Translation	MYEQALAEQAGQVQSPLYGHGQTSGPPTQPPPVHQPKGVSDQEQEDQGTTPNTYAEAEIVYYTIKDQDLPQSLRGMGLKQESPQGEVDTSGRSPHQGGSRGRVHHGNGASDESQEEQAGQVQSPLYGHGQTSEPPTQPPPVHQPKGVSDQEQEDQGTSPNTYAEAEKVYYTIKDQDLPPSLRGVGLKQESPQEEMETSGPPPHQGGSRGRVRHGNGASDESQEEQEACTNVYEEVEDMKRCATCTSEDDLRELSTTVEALKRDQDDLWQLSTTVDALKQVEDMCLLCADHSSETIKTTGDNNSDPFLLVADWKGQTILQVDIISGSKVTLPLEGVDQPYALDYDPLTDYVYWSDGGNKKIKRARRDGSGMETIIDTGNNHTINSLGLALDHAGGNIYWSAVGAKTISVAKKDGSSARTLLTSPDIGYPRDLVLDPRNDDQEYGIYSSDLLGNDIREVLYEPGKYVEGIAVDEDFVYWSSEWFDSSSSSSFQGKIGKLSKSDLTKTVLVDGLKYPVGIYLSTAAPPGVINGHVDVIRHDRTTNSDPNISPYAVTHELTMPYKGKAATQSGQGNDTIPPNSDTYMCHALAMKTLSEDTKTVQPNSDTYMCHALAMKTLSEDTKTVQPNSDTYMCHALAMNTLSEDTKTKAPIQKKIVASTSKEDFPVPLAGRDSPENSADAPALSGNGGTMRESPHPPNEASVHMTPLSNFTHEHNPRPSDPELARDASNPVYGHDLDASNPVYGQGQNTDPDTDLRPAGMQPYAVRYQEEDADDDKDHQPANMEPYAVRYQEEDADDDKDHQPANMEPYAVRYQEEDADDDKDHQPAGMEPYAVRYQDEDADDDKDHQPAGMEPYAGRYQDEDADDDNDHRPASMEPYVDPGMTLSLQGKILALQFHERMTGTCAALENTQGVPHGPREAAGGTTPKQQTDFQARADAAASVPNPMYGVRWVMYASRAGTTPKQQTDWQARADAAASIPNPMYASRADRLYPGGASGCRALCSFIRSQLTYISTGIAVLLGLVAVGLVIFIFINNEEISEQTTTVDVQTTTVDALKRDQDNMSTTVDALKRDQDNMSTTVDALKRDQDDMHQLSTTVDALKRDQDDMRQLSTTVDALKSDQDDMRQLSTTVDALKRDLDKERSRIATSEQRLDATIKTPRDHDSRKNSEPFLLVSCPDEETIIQVDITSGSKITLPLVAVGQPYALDYDPLTDYVYWSDYSNYWNGIIKRARRDGSGMETIIDVKGFHAYGLALDHAGGNIYWSVAVPETISVAKMDGSSARTLLTSPAINMPYELVLDPRNGLMYWVDGYNKGRICRAAMDGSNQTTIITGLNKPQAITIDFNEDRLYYRDQEYGIYSSDLQGNDIREVVHEDGKWVLGIAVDEDFVYWSSGWLSKSDLTKTVLVDGLETQGIYLSTAAPPGVTNVHICASNSEAVIFINKIASGRKEAVTFGVTSSGRSNVLSRIFRKRQRLVLADSYTAIHGRWLEGGREGGRIAREGTTNSDPNISPYAVTHELTMPYKGKAATQSGQGNDTIPPNSDTYMCHALAMKTLSEDTKTVQPNSDTYMCHALAMKTLSEDTKTVQPNSDTYMCHALAMNTLSEDTKTKAPIQKKIVASTSKEDFPVPLAGRDSPENSADAPALSGNGGTMRESPHPPNEASVHMTPLSNLTHEHNPRPSDPELARDASNPVYGHDLDASNPVYGQGQGTDPDTDLRPASMEPYAVRYLEEDADDDKDHRPAGMEPYAEISEQTTTVDVQTTTVDALKRDQDNMSTTVDALKRDQDNMSTTVDALKRDQDDMHQLSTTVDALKRDQDDMRQLSTTVDALKSDQDDMRQLSTTVDALKSDQDDMRQLPTTVDALKRDLDKERSRIATSEQRLDATIKTPRDHDSRKNSEPFLLVADRNKETIIQVDITSGSKLTLPLVGVGWPYVLDFDPLTDFVYWSDGWNINIKRARRDGSGMETITDVKRDAYGLALDHAGGNIYWSVYYAKTISVAKKDGSSARTLLTSPAINHPYGLVLDPRNGLMYWVDVSNKGRICRAAMDGSNQTTIITGLNRPSAITIDFTEDRLYYSDQYYIYSSDLLGNDIRQVLYEDGKWVHGIAVDEDFVYWSSRWWYSSSSSSSWGKIGK

Target ORF information:

RefSeq Version	XM_002598854.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002598854.1

ORF Insert Sequence:

ATGTACGAGC AAGCGCTAGC GGAGCAAGCG GGGCAAGTTC AGTCGCCCCT CTACGGCCAC 
GGCCAAACCA GCGGGCCGCC GACCCAACCC CCTCCTGTCC ACCAGCCCAA AGGCGTTTCT 
GACCAGGAGC AAGAAGACCA AGGAACTACT CCAAACACGT ACGCAGAAGC AGAAATAGTG 
TACTACACAA TCAAGGATCA AGATCTGCCG CAGTCTTTAC GTGGGATGGG GCTGAAGCAG 
GAGTCACCCC AGGGGGAGGT GGATACCAGC GGGCGATCGC CCCACCAGGG TGGTTCCCGC 
GGGCGTGTCC ACCATGGCAA CGGCGCTTCT GACGAGTCGC AAGAAGAGCA AGCGGGGCAA 
GTTCAGTCGC CCCTCTACGG CCACGGCCAA ACCAGCGAGC CGCCGACCCA ACCCCCTCCT 
GTCCACCAGC CCAAAGGCGT TTCTGACCAG GAGCAAGAAG ACCAAGGAAC TTCTCCAAAC 
ACGTACGCAG AAGCAGAAAA AGTGTACTAC ACAATCAAGG ATCAAGATCT GCCGCCGTCT 
TTACGTGGGG TGGGGCTGAA GCAGGAGTCA CCCCAGGAGG AGATGGAAAC CAGCGGGCCA 
CCGCCCCACC AGGGTGGTTC CCGCGGGCGT GTCCGCCATG GCAACGGCGC TTCTGACGAG 
TCGCAAGAAG AGCAAGAAGC GTGTACAAAC GTCTACGAAG AAGTCGAGGA TATGAAGCGT 
TGCGCAACAT GCACTTCTGA AGACGACCTA CGCGAACTGT CCACCACGGT TGAAGCCTTG 
AAGCGCGACC AAGACGACCT ATGGCAACTG TCCACCACTG TTGACGCCTT GAAGCAGGTT 
GAAGACATGT GCCTACTGTG CGCGGATCAC AGTTCCGAGA CGATTAAGAC GACAGGTGAT 
AACAACAGTG ACCCATTCCT CTTGGTCGCT GACTGGAAAG GGCAAACCAT TCTGCAAGTT 
GACATCATTT CCGGATCAAA GGTCACACTT CCGCTTGAGG GTGTGGACCA GCCCTACGCC 
CTGGACTACG ACCCGCTCAC CGACTACGTG TACTGGTCAG ATGGAGGGAA CAAGAAAATC 
AAACGGGCTC GTCGTGATGG AAGTGGCATG GAGACCATCA TAGATACCGG TAACAATCAT 
ACTATCAATA GCCTTGGCCT GGCCCTGGAC CACGCTGGAG GGAATATCTA CTGGTCTGCC 
GTCGGCGCAA AGACTATCTC TGTAGCAAAG AAAGATGGAT CGTCCGCTAG GACACTTCTG 
ACGTCACCAG ACATCGGGTA CCCTAGAGAC CTGGTCCTGG ACCCCAGGAA TGATGATCAG 
GAATACGGCA TCTACAGCTC AGACCTGCTG GGCAACGATA TCCGAGAGGT GCTGTATGAA 
CCCGGGAAGT ATGTGGAGGG AATCGCGGTT GATGAGGACT TCGTCTACTG GTCGTCTGAG 
TGGTTTGATT CATCATCATC ATCATCCTTT CAAGGAAAAA TCGGAAAACT GTCCAAATCC 
GACTTGACCA AGACTGTGCT GGTGGATGGC CTGAAGTACC CAGTGGGCAT CTACCTGTCC 
ACGGCAGCGC CTCCTGGCGT AATCAATGGC CACGTTGACG TCATCAGACA TGACAGAACA 
ACCAACAGCG ATCCCAACAT CAGTCCATAC GCTGTTACAC ACGAGCTGAC CATGCCTTAT 
AAAGGTAAAG CTGCAACCCA GAGCGGACAG GGAAATGACA CCATTCCACC AAACAGCGAT 
ACATACATGT GCCATGCCCT TGCAATGAAG ACACTTTCGG AAGATACCAA GACTGTTCAA 
CCAAACAGCG ATACATACAT GTGCCATGCC CTTGCAATGA AGACACTTTC GGAAGATACC 
AAGACTGTTC AACCGAACAG CGATACATAC ATGTGCCATG CCCTTGCGAT GAATACACTT 
TCGGAAGATA CAAAGACCAA GGCGCCCATT CAGAAAAAAA TAGTGGCTTC AACCAGCAAA 
GAAGATTTCC CAGTTCCGTT AGCAGGACGT GATAGCCCCG AGAACAGTGC TGATGCTCCA 
GCCCTTTCCG GGAATGGCGG TACCATGCGC GAGTCTCCAC ATCCTCCAAA CGAAGCCTCC 
GTGCACATGA CCCCCCTATC TAACTTCACG CACGAGCACA ACCCGCGGCC CTCTGACCCC 
GAGCTTGCAC GTGACGCCTC TAACCCGGTG TACGGGCATG ACCTGGACGC ATCCAACCCG 
GTGTACGGAC AGGGCCAGAA TACGGATCCT GATACTGACT TGCGACCGGC TGGCATGCAG 
CCATATGCCG TCAGATATCA GGAGGAAGAT GCTGATGATG ATAAAGACCA TCAACCAGCT 
AACATGGAGC CATATGCCGT CAGATATCAG GAGGAAGATG CTGATGATGA TAAAGACCAT 
CAACCAGCTA ACATGGAGCC ATATGCCGTC AGATATCAGG AGGAAGATGC TGATGATGAT 
AAAGACCATC AACCGGCTGG CATGGAGCCA TATGCCGTCA GATATCAGGA CGAGGATGCT 
GATGATGATA AAGACCATCA ACCGGCTGGC ATGGAGCCAT ATGCCGGCAG ATATCAGGAC 
GAGGATGCTG ATGATGATAA TGACCATCGA CCGGCTAGCA TGGAGCCATA TGTCGATCCG 
GGAATGACGC TGTCACTTCA GGGAAAGATA CTGGCACTTC AGTTCCACGA AAGGATGACA 
GGAACATGCG CCGCACTCGA AAACACACAG GGTGTTCCCC ACGGTCCTCG GGAAGCAGCT 
GGCGGCACCA CACCCAAGCA GCAGACCGAT TTTCAGGCAC GTGCGGACGC CGCTGCGAGT 
GTACCCAACC CTATGTACGG GGTGCGGTGG GTGATGTACG CCTCCAGAGC AGGCACCACA 
CCCAAGCAGC AGACTGATTG GCAGGCACGT GCGGACGCCG CTGCGAGTAT ACCCAACCCT 
ATGTACGCCT CCAGAGCAGA CCGCCTGTAT CCGGGTGGAG CAAGCGGCTG CCGTGCTCTC 
TGTAGCTTCA TCCGCTCGCA GCTTACCTAC ATCTCCACGG GCATTGCCGT GCTGCTAGGC 
CTGGTCGCTG TGGGACTTGT CATTTTCATT TTCATCAACA ATGAGGAGAT ATCTGAACAG 
ACCACCACTG TTGACGTTCA GACCACCACT GTTGACGCCT TAAAGCGCGA CCAAGACAAC 
ATGTCCACCA CTGTTGATGC CTTGAAGCGC GACCAAGACA ACATGTCCAC CACTGTTGAT 
GCCCTGAAGC GCGACCAAGA CGACATGCAC CAACTGTCCA CCACTGTTGA CGCCTTGAAG 
CGCGACCAAG ACGACATGCG CCAACTGTCC ACCACTGTTG ACGCCTTGAA GAGCGACCAA 
GACGACATGC GCCAACTGTC CACCACTGTT GACGCCTTAA AACGCGACCT GGACAAGGAG 
CGCAGCCGAA TCGCCACCTC GGAGCAACGT CTTGACGCGA CGATTAAGAC GCCAAGAGAC 
CATGACTCCC GTAAGAACAG TGAGCCATTC CTCTTGGTCT CTTGTCCTGA TGAAGAAACC 
ATCATCCAAG TTGACATCAC TTCCGGATCA AAGATCACAC TGCCACTGGT AGCTGTTGGA 
CAGCCCTACG CCCTGGACTA CGACCCGCTC ACCGACTACG TGTACTGGTC AGATTATTCA 
AATTATTGGA ACGGCATCAT CAAACGGGCT CGTCGTGATG GAAGTGGCAT GGAGACCATC 
ATAGATGTAA AGGGATTCCA TGCCTATGGC CTGGCACTGG ACCACGCTGG GGGAAATATC 
TACTGGTCTG TCGCTGTCCC AGAGACTATC TCTGTAGCAA AGATGGATGG ATCGTCCGCT 
AGAACACTTC TGACGTCACC AGCCATCAAT ATGCCTTACG AACTGGTCCT GGACCCCAGG 
AATGGACTCA TGTACTGGGT GGACGGTTAT AACAAAGGCA GGATTTGCCG AGCTGCGATG 
GACGGCAGTA ACCAAACCAC CATCATCACG GGTCTGAATA AGCCCCAAGC CATTACAATA 
GACTTCAATG AGGATCGGCT GTACTACCGT GATCAGGAGT ACGGCATCTA CAGCTCAGAC 
CTACAGGGCA ACGATATCCG AGAAGTGGTG CATGAAGACG GGAAGTGGGT TTTGGGAATC 
GCGGTTGATG AGGACTTCGT CTACTGGTCG TCCGGCTGGC TGTCCAAGTC CGACCTGACC 
AAGACTGTGC TGGTGGATGG CCTGGAGACT CAAGGCATCT ACCTGTCCAC GGCAGCGCCT 
CCTGGCGTAA CCAATGTACA TATTTGTGCC TCCAATTCTG AGGCAGTTAT TTTCATTAAC 
AAAATTGCCT CGGGTAGGAA AGAAGCGGTT ACGTTTGGAG TGACATCTTC AGGAAGGAGT 
AACGTCTTGA GTCGCATCTT CAGGAAGCGG CAACGTCTTG TCCTAGCAGA CTCCTACACG 
GCCATCCACG GGCGTTGGCT GGAGGGAGGA AGGGAGGGAG GGAGGATAGC AAGGGAGGGA 
ACAACCAACA GCGATCCCAA CATCAGTCCA TACGCTGTTA CACACGAGCT GACCATGCCT 
TATAAAGGTA AAGCTGCAAC CCAGAGCGGA CAGGGAAATG ACACCATTCC ACCAAACAGC 
GATACATACA TGTGCCATGC CCTTGCAATG AAGACACTTT CGGAAGATAC CAAGACTGTT 
CAACCAAACA GCGATACATA CATGTGCCAT GCCCTTGCAA TGAAGACACT TTCGGAAGAT 
ACCAAGACTG TTCAACCGAA CAGCGATACA TACATGTGCC ATGCCCTTGC GATGAATACA 
CTTTCGGAAG ATACAAAGAC CAAGGCGCCC ATTCAGAAAA AAATAGTGGC TTCAACCAGC 
AAAGAAGATT TCCCAGTTCC GTTAGCAGGA CGTGATAGCC CCGAGAACAG TGCTGATGCT 
CCAGCCCTTT CCGGGAATGG CGGTACCATG CGCGAGTCTC CACATCCTCC AAACGAAGCC 
TCCGTGCACA TGACCCCCCT ATCTAACCTC ACGCACGAGC ACAACCCGCG GCCCTCTGAC 
CCCGAGCTTG CACGTGACGC CTCCAACCCG GTGTACGGGC ATGACCTGGA CGCATCCAAC 
CCGGTGTACG GACAGGGCCA GGGTACGGAT CCTGATACTG ACTTGCGACC GGCTAGCATG 
GAGCCATATG CCGTCAGATA TCTGGAGGAA GATGCTGATG ATGATAAAGA CCATCGACCA 
GCTGGCATGG AGCCATATGC CGAGATATCT GAACAGACCA CCACTGTTGA CGTTCAGACC 
ACCACTGTTG ACGCCTTAAA GCGCGACCAA GACAACATGT CCACCACTGT TGATGCCTTG 
AAGCGCGACC AAGACAACAT GTCCACCACT GTTGATGCCC TGAAGCGCGA CCAAGACGAC 
ATGCACCAAC TGTCCACCAC TGTTGACGCC TTGAAGCGCG ACCAAGACGA CATGCGTCAA 
CTGTCCACCA CTGTTGACGC CTTGAAGAGC GACCAAGACG ACATGCGCCA ACTGTCCACC 
ACTGTTGACG CCTTGAAGAG CGACCAAGAC GACATGCGCC AACTGCCCAC CACTGTTGAC 
GCCTTAAAAC GCGACCTGGA CAAGGAGCGC AGCCGAATCG CCACCTCGGA GCAGCGTCTT 
GACGCGACGA TTAAGACGCC AAGAGACCAT GACTCCCGTA AGAACAGTGA GCCATTCCTC 
TTGGTCGCTG ATCGTAATAA AGAAACCATC ATCCAAGTTG ACATCACTTC CGGGTCAAAG 
CTCACACTAC CACTGGTAGG TGTTGGATGG CCCTACGTCC TGGACTTCGA CCCGCTCACC 
GACTTCGTGT ACTGGTCAGA TGGATGGAAC ATCAACATCA AACGGGCTCG TCGTGATGGA 
AGTGGCATGG AGACCATCAC AGATGTAAAG AGAGATGCCT ATGGCCTGGC ATTGGACCAC 
GCTGGGGGGA ATATCTACTG GTCTGTCTAT TACGCAAAGA CTATCTCTGT AGCAAAGAAG 
GATGGATCGT CCGCTAGGAC ACTTCTGACG TCACCAGCCA TCAATCACCC TTACGGCCTG 
GTCCTGGACC CCAGGAATGG ACTCATGTAC TGGGTGGACG TTTCTAACAA AGGCAGGATT 
TGCCGAGCTG CGATGGACGG CAGTAACCAA ACCACCATCA TCACGGGTCT GAATCGGCCC 
AGCGCCATCA CAATAGACTT CACTGAGGAT CGGCTGTACT ACAGTGATCA GTATTACATC 
TACAGCTCAG ACCTGCTGGG CAACGATATC CGACAGGTGC TGTATGAAGA CGGGAAGTGG 
GTTCATGGCA TCGCGGTTGA TGAGGACTTC GTCTACTGGT CGTCCAGGTG GTGGTATTCA 
TCATCATCAT CATCCTCTTG GGGAAAAATC GGAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_107340 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

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