BRAFLDRAFT_92090 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_92090
Entrez Gene ID	7213163
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002606024.1	XP_002606070.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_92090 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_92090 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb03470	XM_002606024.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_92090 ( XM_002606024.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002606070.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb03470
Clone ID Related Accession (Same CDS sequence)	XM_002606024.1
Accession Version	XM_002606024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101521). COMPLETENESS: incomplete on both ends.

ATGACAAAAA GCAATCAGCA AAAAAACGGT CCTCTAGATC ACGGTGACAT CAACAAGACG 
CATTTCCATC CACCAGAATT CTTCATCGAA TGGAGGAAGC AAATCTGTCC CTTGGAAGGC 
GAGCCTACTT TCAGAGGCGC CTGGCGTCGA AGACGACGAA GACGGTATTA CGTGTACCCG 
GTCGTGCAAC CGGCTGTGGT ACAGATCGTG ACACCGACAC CGACACGAGA TGTGGAAGCA 
CCTACCTTTC CCAACTGCCC CTCCCCGATT TCTGCCCAAG CGGAAAAGGG AACCGTTTCG 
GCCTTTGTGT CCTGGGCTGC ACCAGGAGCT GAAGACAACT CGGGTGAAGA CGTCCACGTT 
TCACAGACTG TGGGTCAACC TCCAGGGTCA CGATTTGAGG AGGGTTTTCA CACCATAGTT 
TACACAGCGA CGGATTCAGT CATGAACTCC GCAAACTGTT CATTTACAGT CACCGTAACA 
ACTCCGAAGT GCTCCCGATT GCAAACTCTT CAGTATGCAA GCATCCTTTG TGACAACCAA 
TACAACTATG GTTCTACGTG CACCTACACC TGTGATGAGG GATACTTCCT CAACGGAGAT 
CCGACCACAA CATGTGAGCT AGACCTGAGT ACAGATGCCA CTTACTGGAC CCATTCTGCA 
AGCCAACACA ACTGCCAGCC TGTTCAGTGT CCACAACTTC CGTCCATACA AAACGGTGGA 
TGGTTGGTCA ACACAACGTG CAGCAATACC TTCGGCAGCT ACTGCTACTT CCAATGTGAT 
CCTGGCTATA AGCTACAGGG TGATAGCACC GTCTCCTGCA AGGCGTCCAC TCCTGGCCAG 
AGCACGGGAG TCTGGGATGG CAACATCCCC ACTTGCATCG AGTCGACTTG TTCGCCGCTT 
GGACCCATCG CAAATGGGAG AATAGTCTTT GATCAAGACT GTCCTTTCGG TAACGTCTTT 
TCTGCTGGAA GTGTCTGTGG TGTCTCCTGT AGTCCTGGTC ATGTTCTGAT CGGCTCAAGT 
TTCCTGACGT GTCTCTTGGA CGGAACTTGG AATGCCAGTC TTCCATCTTG TGAAGTTATT 
ACCTGCGATA GTGCTGGCCT ACCTACACCG ACCAATGGCT ATAAACTGGG CTGTTCTAAT 
AGTGAGGAAC CCCATGGTAC TACTTGCATC CTCGGATGTA ACACTGGCTA CGAGCCTGCC 
ATCCCAGTAC AACGGACGTG CATAGCAGAT GGTAATGGCG TGGGACAGTG GTCGAATGGG 
ACCATCAATT GCAAAGAGAT CAAGTGTAGG CACCTTCCCA AGCCAAACAA TGGAAACATC 
ACAGCCTGCT ATCATGACGG CGTCAGGACT GAACCAGAGC AGAGTCAGAG TGTCGGAGCA 
GTGTGCAGTA CCATGTGCAA CGAGGGATAC ACGGCTGCGG GATCGGCAGA GAGAACATGT 
CTACCCGGGG GAGGATGGAA TGGCACGGAG CTGCGTTGTC GGGACTTAGC GCCACCAGTG 
TTGCAGTGCC CAGATGAGAT CATACTGGTC GCTAACGAAG GTCAGGACAC AGCTACGGTG 
GACTGGCATT GGGAACCAGT TCTGGCAAAT GATGCCGGAA AAGACATAGT TTCTGCTTTG 
TCTGGATCAA CAACCAGAGA AACCGAATTA CCCGAAGGAC GACATTTTTT CACGTACGAG 
GCAACCGACG CAGCTGGCAA CAGAGCGTCC TGCACTATGG CAATTGACGT CAGAGTGATT 
CGGTGTCCAG GATTGCCGTC AGTCGTCAAC GGTGTCACCA CGCTTGTGGC AGGAAAAGGA 
ACGTGTTCTG GAGGTTCCGT ACGGGGCAGT CAGTGTGAGG TGACGTGTGA TGCAGGCTAC 
AGCATGGCTG ATGGGTCTAC GGTAACGACT AGGACGTGCC ATAGAACTGG AAATGACACC 
TTACAGGCCA GTTGGACAGG CCCTGTACCC GTCTGCACAG GCGTAACATG CCCTCTACCA 
ACTCTCGTCA ACGGTTTTGT CTTTGGCTGT AATCCCCCGG AAGCTATCTA TGGTGCCAGT 
TGTGAGTTCC ATTGCAACCG GGGTTACAAG GGACCGGCAA ACGAGACAGC TGTCACCAGA 
ACGTGTAGAG GAAACGGAAC TTTTACTGGA GAGCCACTTC TGTGTCATCC AATCAAGTGC 
TCCGGTCTTC CCACTGTGGA AAATGGCATG GTCATCCCAG CGACATGTTT GTCTCCTTCT 
GTATCAAACG GGGAGATCTG TATTTTCAAC TGTGACCCAG GGTACTCCAT AATGGGGCCC 
TTCAGCAAGC AATGCCTTAC CGATGGCAGG TGGAGCGATA CCAGGAAAAC ATCGTGCAAA 
GATCATGAGC CACCTAGATT CACCGGCCAA TGCCCAACCT ACGTTAACGC CATCGGAGAC 
GCAGGAAAGA CATCTGCCAC CGTTCTATTC ACTGAACCAG CAGCAACTGA CAACTCCAAC 
TACGTCAATG TATCCAGGGC AGCGAATCAA AGGGGACCGG GTGACGATTT TCCAGAAGGA 
ACGACTGTAG TCACCTATGT TGCTAAGGAC GCTGCGAATA ATAGAGCTCA ATGTGACGTT 
TTTGTGGTCG TGAGAGTGCA CAGATGCCCG ATGGTACAAG CGCCTGCAAG TGGTGCAGTA 
AACTGCTCTG GAGACTTTTA CGGAAGCAAG TGCCAATACA GGTGCAACGA GGGGTACATG 
CTGGATGGCT CGCCTTTCCG CCATTGTGTA CTGTCCGCTG ACGGTTCCAG CTCATATTGG 
AACGGCTCAC AACCGGTATG CAGAGGAGCT TCCTGTAGTC CGTTGGCGCT GCCTGGAGAT 
GTTTCTGCAA CACCGTCCTC CTGCCAAGTG TCTCCCGACG TAGGAGACAC TTGCATCCTC 
TCCTGCAGCA GAACTGGTTA TAGACTTACG CCTCAGTCCG GTCAGTTCGT CATCTGTCTC 
GGAGACGGGC AGTGGTCGTC CAACGTTTCA TCGTTAGTTT GCTCAGACAT AGAGAAACCT 
CGCTTTTCAC AGTGTCCGCA AGACCAAATG TTCTACGCCG ACCGAGGCTC TCAGTCCACC 
TTCGTCAATT GGACTGTATC GGCCATTGAT AACTCAGGAG AAATTCCAAC CGTCACCTGC 
AGTCAATCAC CGGCAGTACT TCCAGCTGGA GATTACCAGG TCTCGTGTAC TGCTACCGAC 
GCTACAGGGA ACACAGAAAC CTGCCTGTTT GACGTCAGAG TCAAAGTAAG ACGATGTCAG 
CAGCTGCTTC CCCCTCTGTA CGGATCGTTT GTCGGCACGT GCGACACCGC CTACGGCAGT 
ACCTGCCAGG TGACCTGCAC GGCTGGCTAC CAACTTGAAG GGTCAGATAC CGCAACATGT 
GAGATGCAGG GAAACAACGC CGTTTGGGAC AGGGCTACTA ATCCTGTTTG CCAAGATATC 
ACAGCACCAA CACTGTTGAC TTGCCCTGGA CCCCAATACG GCACCATCAC CGACAGTTCG 
TTCCTTGGAG TCTCCGTGTC CTTTGACATC CCACGAGCCA CGGACAACTC GGGGGAGACC 
TTAAGCATCA CCAAGCTACC TGTAGACATT ACGTCACCTT ACAACTTTAC GGCAGACACG 
GTCGTGACGT ACGAGTTCCG CGACTCCGGT GGCAATTCGG TCACGTGTAC CTTTATGGTC 
TACATTAAAG GAGAACTTCC TCCAAACGTC GTCTTCTGTC CGGACGACCT CACCATCACA 
GCCACTGATC GCCTTGCTGA AGTGTCCTGG CCAGAGCCCG TGTTCGACGA TCCAACGGGC 
GGCGACTTGG ATGTCTCCGC CAACATAGCA AATAACGGCT CCCTCCCCTG GGGAGACCAC 
TCCGTCACTT ATACTGCCAC CAACGTCGAC AACGGCAAAA GTGTCACCTG TACTTTTCTT 
GTCATCGTGC GACCTGTTGC ATGTCCCCCG CTGAATCCTC CACTTCACGG TGCCCTGGCC 
TGCGATACAT GGGTGTACGG CAAATTCTGC TCCATGTTCT GCAGCGCTCA ATATGATATT 
CCCAGGGGGT CAAACCCAGG TGACAAGTAC ATATGCGGGG CTAGCGGAAT TTGGTCACCC 
CCTCCCCCTG TGCCTGATTG TGTCAGTAGC TTCAGGCCAA ATCGTTTCGT CTTGCCTTCC 
GAGCTACATT ACTTCTCGGG AGACTGCTCG GATCCGAGCG TCCAAGCCGA TATCGCCCAG 
CGGTTCGTCA CCATTTTGAA CGGCTCTGAC TTTGGCAGCG CTTGTTCAGA TTCTGTAGGT 
TGTTCATGGG AAAACGTAGA AGTTGTCTGC GGGGCCAATT CGAGACGACG CTCTCTTGGG 
AGACAAAGCA AGCTGGTAAC AGGACAGATG ACCGACATTA GCAGTCGTTC ATCGGGACAG 
TACCTACTCA CGATGAAGTT TTCCTTGGTA GTGAACATGA CTGATGACGA CCCCAACCTG 
TCGTCAGTAG ATAGGTATTA TGCAGCTGAC GATCAGCTTC TCATGATGAC AGATGTCATT 
CAGGCGGAAA TCTATTCCGG AGACTTTGAT GTAAGTGTCC CAGGACTCTC TCTTCAAGTC 
GATCAGAACT CTCTGACGTA CGGTTGGTCT GAGCTAGACT GTCCCAAAGG GTATGCAGAA 
CAGCCCGACA GCATGAACTG TGGTGTGTAC CTTTTAGTTG TTATTTCGTT GTTTCGTTGG 
TATTGTAATT TAATTGGTGT TTTTGATTAT CCTGGTTATC CTGAGTGTGT TGGATGTCCC 
ACTGGCACGT TCTATGACGA AGACGCGGAC GACTGTCTCA AGTGTTCTGT CGGCTACTAC 
CAAGACGAAG AGGGCCAGTT CCAATGCAAG ACCTGCCCTC TGGGAACTTC AACCATCGAT 
GAAGGCGCAA GGAACCTCAC GGAATGTCTA GACTTATGTC CTCCAGGAAG CTACTCCAAC 
ACACGTGTCG TGCCTTGCAC CAAGTGTGAC ATCGGCTACT ACCAAGACCA GGAGGGGCAT 
ACGATCTGCA ACAGATGCAA TGCTTCTTTC TCTACCCTGA CAATCGGTTC CTCAAGTGAA 
TCTGATTGCA AAGAACTGGA TGCATGTGTC AGCAATCCGT GTGGCAATGG CGGCGTCTGT 
AACAGACTGA TTGACGGTTA CACGTGCGTC TGTCTGTCGG GTTTCATCGG CGACAACTGT 
GAAGTGAACA TAGACGACTG CGCGAGAAAT CCCTGTCTAA ACAACGGAAC CTGCGTAGAT 
GGGATTGACA CTTACTCTTG CGTATGCAGC CAGGGTTATA CAGGTAACAC TTGTGAGGAA 
GACATTGACG ACTGTCAACA TCTACCGTGC CTCAATAACG GGGCATGTGA AGATCTAGTA 
GGCAGTTTTA TCTGTCACTG TCAACCGGGC TTTATGGGAG CCACGTGTGA AATTACAGTT 
GATGCTTGCT ACCATCATGA ATGTAAAAAC AGTGCAACGT GTGTTAGTCA GGATACGTCA 
TACGAGTGTG TCTGTCCAAA TGGCTTCACA GGAGATTTGT GTGAGACAAA CATAGATGAC 
TGTCGCGGAA ACCCTTGTCT TCACAACGGC GTGTGCGTTG ATGGTGTCAT GGACTTCACA 
TGTCTGTGCA CCATGGGCTA CACGGGTAGT TTGTGCGAGG TTGATGTGGA TCTTTGTGAC 
TCGGGACCTT GTCTAAACAG CGGTACTTGC GTAGACCTTG GAGACGACTT TGCTTGCCAG 
TGTGTCGAAG GACTGTCGGG GAAAACATGC GAGTCACCTA GAGATCCGTG TTCAGTTCAA 
CCGTGTCTCA ACGGTGGCAG CTGTACGGTG CAACCGCAAA CGGGTGACTA CGTCTGTAGC 
TGTGCACAAG GCTTCGCAGG CATACAATGT GAAATAGACA TCGACGAGTG CGCATCTGGA 
CCCTGTTCGA ATGACGCTAT TTGTTTAGAC GGGATAAATC AGTATTCCTG CGCCTGCACC 
AATGGCTTTG TTGGTCACAA CTGTGAAGAA GCAGTAGACC ACTGTGCTTC ATCCCCGTGC 
GGCAACAACT CCACCTGCCT CTCTGAACGA GATGGGTACC GCTGTATCTG TCCTCCTACA 
GCCACAGGAC CGACATGTGG TACTCCTGTA GATCTCTGTA CATCATCGCC ATGTGTAAAC 
AATGGGTCAT GTGTAGTAGA TGGCAACGGC TACACCTGTT ATTGCAGTCC TGATTTCACA 
GGACCCACGT GTGATACAGA AGAGGATAGT TGCGAGGATG ACCCTTGCGC AAACGGCGGG 
CTATGTACCG ATGGCTTTGG TACCTTCTCC TGCGCCTGCC CCGACGGCTA CACTGGCTTA 
ACGTGCGAAC AGGAGATCAA CGAATGCACC AGCAATCCGT GTCAAAACTC GGGCGTATGC 
GTCGACAAGG AAGCCGGATA CGATTGCTTG TGCCCCAAAG GTACCTCAGG CAACCATTGC 
GAAGAGAACT TTGACGACTG CCACAACGTA ACTTGCCTTA ATGGCGGTTC GTGTATTGAC 
CTTGTAAGCG CATTCCTCTG TAACTGCTCC CCAGGGTACG AAGGGAAATT TTGCGAGAGC 
GAAATCAACG AGTGTAACAT CTATCCCTGC AGAAACGCAA GATCCTGCCG GGATCTTCTG 
AACGACTATG AGTGTGAATG TGAACCAGGA TGGACGGGTA AGAACTGCGA GACGAATATC 
GATGACTGTT TCCCGAATCC TTGTGGCAAC GGCGGGGTAT GTCATGACCG TGTGAACAGC 
TACACATGCT CGTGCTTGGG CGGGTTTTCG GGGCTCCATT GTGAAGTCAA CATTGACGAA 
TGCATGGAGG TTCAATGCGA AAACAATGGT ACTTGCGTTG ACGGTGTAGG GACTGCGTCT 
TGCGTCTGCG GGTTAGGCTG GACAGGTACA AACTGTCAAG TGGAGATTGA CGAGTGTAGC 
TTGGAGCCTT GCATGAACGG AGGAGGGTGT GTTGACACCA TCGGGTCATT TGTCTGCAAC 
TGCGTCGACG GTTTTACGGG AAGGCTCTGT GAAGAAGACA TAAACGATTG CGACATACCA 
AGATGCATGA ACGGCGGAAC ATGCGTAGAT CAGGTAAATG GCATTATATA CGAGGCATTC 
ACTTGTAACG TGCGTGGTCC GGTGGTTAAG GTTGCCAGGT ACATGTGTAG GTGCCCTCCA 
GGCTATACCG GTACAAACTG TGAAGTCAAC ATCAACGAAT GTTCGTCACA GCCTTGCAGG 
AACGGCGGGC AATGTATTGA TGAATCCGAT GGTTTTATCT GCGTCTGTCC TCAGGGATTT 
TCGGGTCGAA CATGTGATGT CAACATAGAC GACTGCATTC CCAACATGTG CGAGAATGGA 
GCAACCTGTC TGGACGGGAT CGGTGACTAC ACCTGCATTT GCAAACTAGG ATTCAGTGGC 
CGTCGTTGTG AAGTAGATGT TGATGACTGT GCCAGTCGTC CTTGTGAGAA CACCGCAACT 
TGCCTGGATG GAGTCAATGA GTACACTTGC CTATGCCCAC CGGGATATTC TGGGGTGAGG 
TGTGAATATG CCCTTCCTAC AGACTTCGAC ATCCATGTGA CCTCATCAGA CAGCAATCAC 
TACTCAGAGG TTTCCAAGCA AAACATCCAC AGCATGTCTG CCATTGCCTG GTGGATGCGT 
GTAATGGACG CGCCAAACAA CTACGCCCCG GTAACAGTGG CCGTCACAAG GAATAACAGT 
GACGCGAGGG AACCTTTGTT CATTGTACAA AACCCAAACC GCATTGAGCT TGTTTTATCG 
GGTACTATTA TCCAGTCGTC TGTGAACTGT GCCGATGGCG GATGGCATCA CGTGGCCGTC 
TCATATGAGC CTGCGTCAGA GGTACTGGCT CTTTACGTCG ACGGAATGAT GGAAAACACA 
ACCCGCGTAC CCAGTCTCCA AAACTCGTCA CCAGCCGACG GTTTTGTCGT CTCTGTGGCC 
ACTGGGGTGA CCAATGCTAA CATCGGAGAC TCCGCGTTTC AAGGGGAGAT CAGCCAGCTG 
AACGCATGGG CACGAGAGTT GTCGCAGTCC GAGATTGTGG AACTCAGCAG TACCTGTCAG 
ACAGTGGCCG TAGGGGATGC TGTTGCGTGG TCTGACTTCG TCGATGCTAC ACAGACGGAT 
CCACAGTATG GTGCCATTTC GTCGCCGTCA GTTTGTGACT CTGAAGACCA ATGTGAAAGC 
TCTCCGTGTT TGAACGGTGG TACTTGCAGG GACGGATATC TTACCTTCAC CTGCACGTGT 
CCTCACAGCC ACGTGGGACA ACGTTGTGAG ACTCAACTCA ACCCCTGTGA TGGGAACCTG 
TGTCAGAACG GTGCCGCCTG CGTAGCTGAC GTGACAAGCA TGACATATAC CTGCAGCTGT 
CCTGAAGGGT GTTCAGGAGA TTTGTGTAAC GTGCTGTCAG TTAATGGTGG CTGGAGCGAG 
TGGAGCCAGT GGAGTGCTTG TACTGCCACT TGTGGCAACT CAACACGCTC CCGACAGCGT 
CTGTGCAACA ACCCTCCTCC GCTAGGCGGC GGGAACCCAT GTACCGGAGT TTCTCTGGAG 
ACAGAGGCGT GTGTTAACAT ACCAGACTGT ACTCGGTGCA CCGAGTTGCG GCATCCAGAA 
AATGGCTACC TGGTCTGCAG TCCCGACGGT GTAGACACTA AGTGTGAGGT TCACTGCAGA 
GCTGGGTTCA TGATGGTGGT CAACCCTGAT CTGTACGTAA ATCTTCGCTG TGGTCCCTCT 
ACATGGTACA CTTGGACGCA CCAAGACCTC ACAAATCTTC CAGCAAGGAT ACCTGGCTGT 
ACAAAGAAAG AGTTACCAAC CTCAGTAACT GTGGACTACG TCATTTGTTA CAAGAATGTC 
AGCGAAGACC CTTTCGCAAC CTTGTTTACA CAACAGATCC TGGGTCCCAG TGCTTCGTTC 
CTCAATGAAC AAACTTACGA ACATGCCTTT GATTCCGTTG TAACACGAAA TCTTTTGTCT 
CTTGACTGTA TTACCAGCCG TAACTGTCAC AAAGATGGTT CTGAAGTTCG CTTCGACAAC 
GTCACGACAG GCAACAATAC CAGATTGGAC GTTGTCGGTC ATATCTTCAT GTCTACGCAG 
GTTCCGCAGG TGTCAGATCC TGTTCTGACG CTTGCTCAGT CGTCAAAATA CAGCTTCCCA 
ATGGACGTGA TTTCGGGCGG TTTTGTTGCT GGAAACCCTG CGTTAACCTC CGTTGGAAAC 
CTAAGTCTTA TTGATGGCAT ATCGGGTAAG GCAATATCTC TAGACGGGCA GGCGCAATAC 
CTGAATGCAG GGCAATTTCC CGGAGAATGT CTTGGTTCGT CTGTGTATTG TCCGAATGGT 
TATACAATCA CAGCTTGGCT CCGCGTCAAC AGTGTGCCAT CATCACCATC ACATGACGTG 
TACTATCTTA GCAACGGCGG TCAGACTAGT TCTTCGAGGG GATTTGCCGT CGGATACAAA 
GGTGGCTTCT TCGCGCGGGT AAGCTTCGAC AGTCGCTATC GTTCGCTCGC CTTTTCGGTA 
GATTTGCACA CTTGGTTCAA CACAGCCCTG ACCTGGTCAC CCGGGGGAGA TCCCACACTT 
TACATCAATG GTACTCTCGT TAACGCGACG GTCACCGACG GTAGCCTGTC AGCCTTGACA 
TCAGATACAC GAACGCAGTT CCTCGTGGGT GCTCCCAACA ACATAGATAA CCCATCTTCG 
GGTCATCTTG GACATACCGA TGTCGACGAT GTCCTGTTCT GGGACCGGGT CCTGACGCAG 
GAAGAGATCC GAGATCTAAT GTCTGCCGAG CCAGTAGCTG TTGCAAGGAT CTGCCGAGGA 
CAACATCTGA CGCTAAGTTG CGAACCAGAA GAAGTCATCA CCATCCACTC AGCTACGTTC 
AAGGTATCAA CGCCTTCTGA GTGTGGAGCT TTGGACTCTT CACAGATAGA CAGCTGTGTG 
GATGATGCCG CTTTGGAATT TACCTTGAAC CTCTGTGACG GGCAGAGAAG CTGCCACCTC 
CATGCCACAG ATGAGGTCTT TGGGAATGAC TGTGTGGAGA CAAGAAATTC ATTCGAGGTT 
CTCTATACAT GCGACACGGG CCGCTTCAAT GACAAGGACA TAATCATGGA GAACATTGAA 
CAAGATTGGG AAACCACATT GAAAAGCCTG AGCCAAATGG TCGAAAATGG TGACTTTGAC 
CTGAACGTAC TGCATCAGGA CATTCCTGCA GACAAGAACA ACACAGAAGA GATAGAAATA 
CAACTAACCT GCACTGGCAA CATGGTTGGC ATGGGACTGT GTGACGCCTG TTTCCCTGGA 
TCGTTCTACA ATGGCCGGGA ATGCGTCCCG TGCCCACAGG GACAATTTCA GAGTCCGACA 
ACCCCAACCA AGTGCGAATC TTGTCCGCCG GGCTACACTA CTGTAGGATT CGGAAGCGTC 
GATGTGACTG CATGCTACGT GTACACGTGT ACATGTGACT TCAGCGTCCG GACGACTGAT 
CCAGATCTAC AGGGCAACGT ACGGTTCAAG TTCCGCTCGG TCAGGGCGTG TGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002606070.1
CDS	1..10497
Translation	MTKSNQQKNGPLDHGDINKTHFHPPEFFIEWRKQICPLEGEPTFRGAWRRRRRRRYYVYPVVQPAVVQIVTPTPTRDVEAPTFPNCPSPISAQAEKGTVSAFVSWAAPGAEDNSGEDVHVSQTVGQPPGSRFEEGFHTIVYTATDSVMNSANCSFTVTVTTPKCSRLQTLQYASILCDNQYNYGSTCTYTCDEGYFLNGDPTTTCELDLSTDATYWTHSASQHNCQPVQCPQLPSIQNGGWLVNTTCSNTFGSYCYFQCDPGYKLQGDSTVSCKASTPGQSTGVWDGNIPTCIESTCSPLGPIANGRIVFDQDCPFGNVFSAGSVCGVSCSPGHVLIGSSFLTCLLDGTWNASLPSCEVITCDSAGLPTPTNGYKLGCSNSEEPHGTTCILGCNTGYEPAIPVQRTCIADGNGVGQWSNGTINCKEIKCRHLPKPNNGNITACYHDGVRTEPEQSQSVGAVCSTMCNEGYTAAGSAERTCLPGGGWNGTELRCRDLAPPVLQCPDEIILVANEGQDTATVDWHWEPVLANDAGKDIVSALSGSTTRETELPEGRHFFTYEATDAAGNRASCTMAIDVRVIRCPGLPSVVNGVTTLVAGKGTCSGGSVRGSQCEVTCDAGYSMADGSTVTTRTCHRTGNDTLQASWTGPVPVCTGVTCPLPTLVNGFVFGCNPPEAIYGASCEFHCNRGYKGPANETAVTRTCRGNGTFTGEPLLCHPIKCSGLPTVENGMVIPATCLSPSVSNGEICIFNCDPGYSIMGPFSKQCLTDGRWSDTRKTSCKDHEPPRFTGQCPTYVNAIGDAGKTSATVLFTEPAATDNSNYVNVSRAANQRGPGDDFPEGTTVVTYVAKDAANNRAQCDVFVVVRVHRCPMVQAPASGAVNCSGDFYGSKCQYRCNEGYMLDGSPFRHCVLSADGSSSYWNGSQPVCRGASCSPLALPGDVSATPSSCQVSPDVGDTCILSCSRTGYRLTPQSGQFVICLGDGQWSSNVSSLVCSDIEKPRFSQCPQDQMFYADRGSQSTFVNWTVSAIDNSGEIPTVTCSQSPAVLPAGDYQVSCTATDATGNTETCLFDVRVKVRRCQQLLPPLYGSFVGTCDTAYGSTCQVTCTAGYQLEGSDTATCEMQGNNAVWDRATNPVCQDITAPTLLTCPGPQYGTITDSSFLGVSVSFDIPRATDNSGETLSITKLPVDITSPYNFTADTVVTYEFRDSGGNSVTCTFMVYIKGELPPNVVFCPDDLTITATDRLAEVSWPEPVFDDPTGGDLDVSANIANNGSLPWGDHSVTYTATNVDNGKSVTCTFLVIVRPVACPPLNPPLHGALACDTWVYGKFCSMFCSAQYDIPRGSNPGDKYICGASGIWSPPPPVPDCVSSFRPNRFVLPSELHYFSGDCSDPSVQADIAQRFVTILNGSDFGSACSDSVGCSWENVEVVCGANSRRRSLGRQSKLVTGQMTDISSRSSGQYLLTMKFSLVVNMTDDDPNLSSVDRYYAADDQLLMMTDVIQAEIYSGDFDVSVPGLSLQVDQNSLTYGWSELDCPKGYAEQPDSMNCGVYLLVVISLFRWYCNLIGVFDYPGYPECVGCPTGTFYDEDADDCLKCSVGYYQDEEGQFQCKTCPLGTSTIDEGARNLTECLDLCPPGSYSNTRVVPCTKCDIGYYQDQEGHTICNRCNASFSTLTIGSSSESDCKELDACVSNPCGNGGVCNRLIDGYTCVCLSGFIGDNCEVNIDDCARNPCLNNGTCVDGIDTYSCVCSQGYTGNTCEEDIDDCQHLPCLNNGACEDLVGSFICHCQPGFMGATCEITVDACYHHECKNSATCVSQDTSYECVCPNGFTGDLCETNIDDCRGNPCLHNGVCVDGVMDFTCLCTMGYTGSLCEVDVDLCDSGPCLNSGTCVDLGDDFACQCVEGLSGKTCESPRDPCSVQPCLNGGSCTVQPQTGDYVCSCAQGFAGIQCEIDIDECASGPCSNDAICLDGINQYSCACTNGFVGHNCEEAVDHCASSPCGNNSTCLSERDGYRCICPPTATGPTCGTPVDLCTSSPCVNNGSCVVDGNGYTCYCSPDFTGPTCDTEEDSCEDDPCANGGLCTDGFGTFSCACPDGYTGLTCEQEINECTSNPCQNSGVCVDKEAGYDCLCPKGTSGNHCEENFDDCHNVTCLNGGSCIDLVSAFLCNCSPGYEGKFCESEINECNIYPCRNARSCRDLLNDYECECEPGWTGKNCETNIDDCFPNPCGNGGVCHDRVNSYTCSCLGGFSGLHCEVNIDECMEVQCENNGTCVDGVGTASCVCGLGWTGTNCQVEIDECSLEPCMNGGGCVDTIGSFVCNCVDGFTGRLCEEDINDCDIPRCMNGGTCVDQVNGIIYEAFTCNVRGPVVKVARYMCRCPPGYTGTNCEVNINECSSQPCRNGGQCIDESDGFICVCPQGFSGRTCDVNIDDCIPNMCENGATCLDGIGDYTCICKLGFSGRRCEVDVDDCASRPCENTATCLDGVNEYTCLCPPGYSGVRCEYALPTDFDIHVTSSDSNHYSEVSKQNIHSMSAIAWWMRVMDAPNNYAPVTVAVTRNNSDAREPLFIVQNPNRIELVLSGTIIQSSVNCADGGWHHVAVSYEPASEVLALYVDGMMENTTRVPSLQNSSPADGFVVSVATGVTNANIGDSAFQGEISQLNAWARELSQSEIVELSSTCQTVAVGDAVAWSDFVDATQTDPQYGAISSPSVCDSEDQCESSPCLNGGTCRDGYLTFTCTCPHSHVGQRCETQLNPCDGNLCQNGAACVADVTSMTYTCSCPEGCSGDLCNVLSVNGGWSEWSQWSACTATCGNSTRSRQRLCNNPPPLGGGNPCTGVSLETEACVNIPDCTRCTELRHPENGYLVCSPDGVDTKCEVHCRAGFMMVVNPDLYVNLRCGPSTWYTWTHQDLTNLPARIPGCTKKELPTSVTVDYVICYKNVSEDPFATLFTQQILGPSASFLNEQTYEHAFDSVVTRNLLSLDCITSRNCHKDGSEVRFDNVTTGNNTRLDVVGHIFMSTQVPQVSDPVLTLAQSSKYSFPMDVISGGFVAGNPALTSVGNLSLIDGISGKAISLDGQAQYLNAGQFPGECLGSSVYCPNGYTITAWLRVNSVPSSPSHDVYYLSNGGQTSSSRGFAVGYKGGFFARVSFDSRYRSLAFSVDLHTWFNTALTWSPGGDPTLYINGTLVNATVTDGSLSALTSDTRTQFLVGAPNNIDNPSSGHLGHTDVDDVLFWDRVLTQEEIRDLMSAEPVAVARICRGQHLTLSCEPEEVITIHSATFKVSTPSECGALDSSQIDSCVDDAALEFTLNLCDGQRSCHLHATDEVFGNDCVETRNSFEVLYTCDTGRFNDKDIIMENIEQDWETTLKSLSQMVENGDFDLNVLHQDIPADKNNTEEIEIQLTCTGNMVGMGLCDACFPGSFYNGRECVPCPQGQFQSPTTPTKCESCPPGYTTVGFGSVDVTACYVYTCTCDFSVRTTDPDLQGNVRFKFRSVRACG

Target ORF information:

RefSeq Version	XM_002606024.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002606024.1

ORF Insert Sequence:

ATGACAAAAA GCAATCAGCA AAAAAACGGT CCTCTAGATC ACGGTGACAT CAACAAGACG 
CATTTCCATC CACCAGAATT CTTCATCGAA TGGAGGAAGC AAATCTGTCC CTTGGAAGGC 
GAGCCTACTT TCAGAGGCGC CTGGCGTCGA AGACGACGAA GACGGTATTA CGTGTACCCG 
GTCGTGCAAC CGGCTGTGGT ACAGATCGTG ACACCGACAC CGACACGAGA TGTGGAAGCA 
CCTACCTTTC CCAACTGCCC CTCCCCGATT TCTGCCCAAG CGGAAAAGGG AACCGTTTCG 
GCCTTTGTGT CCTGGGCTGC ACCAGGAGCT GAAGACAACT CGGGTGAAGA CGTCCACGTT 
TCACAGACTG TGGGTCAACC TCCAGGGTCA CGATTTGAGG AGGGTTTTCA CACCATAGTT 
TACACAGCGA CGGATTCAGT CATGAACTCC GCAAACTGTT CATTTACAGT CACCGTAACA 
ACTCCGAAGT GCTCCCGATT GCAAACTCTT CAGTATGCAA GCATCCTTTG TGACAACCAA 
TACAACTATG GTTCTACGTG CACCTACACC TGTGATGAGG GATACTTCCT CAACGGAGAT 
CCGACCACAA CATGTGAGCT AGACCTGAGT ACAGATGCCA CTTACTGGAC CCATTCTGCA 
AGCCAACACA ACTGCCAGCC TGTTCAGTGT CCACAACTTC CGTCCATACA AAACGGTGGA 
TGGTTGGTCA ACACAACGTG CAGCAATACC TTCGGCAGCT ACTGCTACTT CCAATGTGAT 
CCTGGCTATA AGCTACAGGG TGATAGCACC GTCTCCTGCA AGGCGTCCAC TCCTGGCCAG 
AGCACGGGAG TCTGGGATGG CAACATCCCC ACTTGCATCG AGTCGACTTG TTCGCCGCTT 
GGACCCATCG CAAATGGGAG AATAGTCTTT GATCAAGACT GTCCTTTCGG TAACGTCTTT 
TCTGCTGGAA GTGTCTGTGG TGTCTCCTGT AGTCCTGGTC ATGTTCTGAT CGGCTCAAGT 
TTCCTGACGT GTCTCTTGGA CGGAACTTGG AATGCCAGTC TTCCATCTTG TGAAGTTATT 
ACCTGCGATA GTGCTGGCCT ACCTACACCG ACCAATGGCT ATAAACTGGG CTGTTCTAAT 
AGTGAGGAAC CCCATGGTAC TACTTGCATC CTCGGATGTA ACACTGGCTA CGAGCCTGCC 
ATCCCAGTAC AACGGACGTG CATAGCAGAT GGTAATGGCG TGGGACAGTG GTCGAATGGG 
ACCATCAATT GCAAAGAGAT CAAGTGTAGG CACCTTCCCA AGCCAAACAA TGGAAACATC 
ACAGCCTGCT ATCATGACGG CGTCAGGACT GAACCAGAGC AGAGTCAGAG TGTCGGAGCA 
GTGTGCAGTA CCATGTGCAA CGAGGGATAC ACGGCTGCGG GATCGGCAGA GAGAACATGT 
CTACCCGGGG GAGGATGGAA TGGCACGGAG CTGCGTTGTC GGGACTTAGC GCCACCAGTG 
TTGCAGTGCC CAGATGAGAT CATACTGGTC GCTAACGAAG GTCAGGACAC AGCTACGGTG 
GACTGGCATT GGGAACCAGT TCTGGCAAAT GATGCCGGAA AAGACATAGT TTCTGCTTTG 
TCTGGATCAA CAACCAGAGA AACCGAATTA CCCGAAGGAC GACATTTTTT CACGTACGAG 
GCAACCGACG CAGCTGGCAA CAGAGCGTCC TGCACTATGG CAATTGACGT CAGAGTGATT 
CGGTGTCCAG GATTGCCGTC AGTCGTCAAC GGTGTCACCA CGCTTGTGGC AGGAAAAGGA 
ACGTGTTCTG GAGGTTCCGT ACGGGGCAGT CAGTGTGAGG TGACGTGTGA TGCAGGCTAC 
AGCATGGCTG ATGGGTCTAC GGTAACGACT AGGACGTGCC ATAGAACTGG AAATGACACC 
TTACAGGCCA GTTGGACAGG CCCTGTACCC GTCTGCACAG GCGTAACATG CCCTCTACCA 
ACTCTCGTCA ACGGTTTTGT CTTTGGCTGT AATCCCCCGG AAGCTATCTA TGGTGCCAGT 
TGTGAGTTCC ATTGCAACCG GGGTTACAAG GGACCGGCAA ACGAGACAGC TGTCACCAGA 
ACGTGTAGAG GAAACGGAAC TTTTACTGGA GAGCCACTTC TGTGTCATCC AATCAAGTGC 
TCCGGTCTTC CCACTGTGGA AAATGGCATG GTCATCCCAG CGACATGTTT GTCTCCTTCT 
GTATCAAACG GGGAGATCTG TATTTTCAAC TGTGACCCAG GGTACTCCAT AATGGGGCCC 
TTCAGCAAGC AATGCCTTAC CGATGGCAGG TGGAGCGATA CCAGGAAAAC ATCGTGCAAA 
GATCATGAGC CACCTAGATT CACCGGCCAA TGCCCAACCT ACGTTAACGC CATCGGAGAC 
GCAGGAAAGA CATCTGCCAC CGTTCTATTC ACTGAACCAG CAGCAACTGA CAACTCCAAC 
TACGTCAATG TATCCAGGGC AGCGAATCAA AGGGGACCGG GTGACGATTT TCCAGAAGGA 
ACGACTGTAG TCACCTATGT TGCTAAGGAC GCTGCGAATA ATAGAGCTCA ATGTGACGTT 
TTTGTGGTCG TGAGAGTGCA CAGATGCCCG ATGGTACAAG CGCCTGCAAG TGGTGCAGTA 
AACTGCTCTG GAGACTTTTA CGGAAGCAAG TGCCAATACA GGTGCAACGA GGGGTACATG 
CTGGATGGCT CGCCTTTCCG CCATTGTGTA CTGTCCGCTG ACGGTTCCAG CTCATATTGG 
AACGGCTCAC AACCGGTATG CAGAGGAGCT TCCTGTAGTC CGTTGGCGCT GCCTGGAGAT 
GTTTCTGCAA CACCGTCCTC CTGCCAAGTG TCTCCCGACG TAGGAGACAC TTGCATCCTC 
TCCTGCAGCA GAACTGGTTA TAGACTTACG CCTCAGTCCG GTCAGTTCGT CATCTGTCTC 
GGAGACGGGC AGTGGTCGTC CAACGTTTCA TCGTTAGTTT GCTCAGACAT AGAGAAACCT 
CGCTTTTCAC AGTGTCCGCA AGACCAAATG TTCTACGCCG ACCGAGGCTC TCAGTCCACC 
TTCGTCAATT GGACTGTATC GGCCATTGAT AACTCAGGAG AAATTCCAAC CGTCACCTGC 
AGTCAATCAC CGGCAGTACT TCCAGCTGGA GATTACCAGG TCTCGTGTAC TGCTACCGAC 
GCTACAGGGA ACACAGAAAC CTGCCTGTTT GACGTCAGAG TCAAAGTAAG ACGATGTCAG 
CAGCTGCTTC CCCCTCTGTA CGGATCGTTT GTCGGCACGT GCGACACCGC CTACGGCAGT 
ACCTGCCAGG TGACCTGCAC GGCTGGCTAC CAACTTGAAG GGTCAGATAC CGCAACATGT 
GAGATGCAGG GAAACAACGC CGTTTGGGAC AGGGCTACTA ATCCTGTTTG CCAAGATATC 
ACAGCACCAA CACTGTTGAC TTGCCCTGGA CCCCAATACG GCACCATCAC CGACAGTTCG 
TTCCTTGGAG TCTCCGTGTC CTTTGACATC CCACGAGCCA CGGACAACTC GGGGGAGACC 
TTAAGCATCA CCAAGCTACC TGTAGACATT ACGTCACCTT ACAACTTTAC GGCAGACACG 
GTCGTGACGT ACGAGTTCCG CGACTCCGGT GGCAATTCGG TCACGTGTAC CTTTATGGTC 
TACATTAAAG GAGAACTTCC TCCAAACGTC GTCTTCTGTC CGGACGACCT CACCATCACA 
GCCACTGATC GCCTTGCTGA AGTGTCCTGG CCAGAGCCCG TGTTCGACGA TCCAACGGGC 
GGCGACTTGG ATGTCTCCGC CAACATAGCA AATAACGGCT CCCTCCCCTG GGGAGACCAC 
TCCGTCACTT ATACTGCCAC CAACGTCGAC AACGGCAAAA GTGTCACCTG TACTTTTCTT 
GTCATCGTGC GACCTGTTGC ATGTCCCCCG CTGAATCCTC CACTTCACGG TGCCCTGGCC 
TGCGATACAT GGGTGTACGG CAAATTCTGC TCCATGTTCT GCAGCGCTCA ATATGATATT 
CCCAGGGGGT CAAACCCAGG TGACAAGTAC ATATGCGGGG CTAGCGGAAT TTGGTCACCC 
CCTCCCCCTG TGCCTGATTG TGTCAGTAGC TTCAGGCCAA ATCGTTTCGT CTTGCCTTCC 
GAGCTACATT ACTTCTCGGG AGACTGCTCG GATCCGAGCG TCCAAGCCGA TATCGCCCAG 
CGGTTCGTCA CCATTTTGAA CGGCTCTGAC TTTGGCAGCG CTTGTTCAGA TTCTGTAGGT 
TGTTCATGGG AAAACGTAGA AGTTGTCTGC GGGGCCAATT CGAGACGACG CTCTCTTGGG 
AGACAAAGCA AGCTGGTAAC AGGACAGATG ACCGACATTA GCAGTCGTTC ATCGGGACAG 
TACCTACTCA CGATGAAGTT TTCCTTGGTA GTGAACATGA CTGATGACGA CCCCAACCTG 
TCGTCAGTAG ATAGGTATTA TGCAGCTGAC GATCAGCTTC TCATGATGAC AGATGTCATT 
CAGGCGGAAA TCTATTCCGG AGACTTTGAT GTAAGTGTCC CAGGACTCTC TCTTCAAGTC 
GATCAGAACT CTCTGACGTA CGGTTGGTCT GAGCTAGACT GTCCCAAAGG GTATGCAGAA 
CAGCCCGACA GCATGAACTG TGGTGTGTAC CTTTTAGTTG TTATTTCGTT GTTTCGTTGG 
TATTGTAATT TAATTGGTGT TTTTGATTAT CCTGGTTATC CTGAGTGTGT TGGATGTCCC 
ACTGGCACGT TCTATGACGA AGACGCGGAC GACTGTCTCA AGTGTTCTGT CGGCTACTAC 
CAAGACGAAG AGGGCCAGTT CCAATGCAAG ACCTGCCCTC TGGGAACTTC AACCATCGAT 
GAAGGCGCAA GGAACCTCAC GGAATGTCTA GACTTATGTC CTCCAGGAAG CTACTCCAAC 
ACACGTGTCG TGCCTTGCAC CAAGTGTGAC ATCGGCTACT ACCAAGACCA GGAGGGGCAT 
ACGATCTGCA ACAGATGCAA TGCTTCTTTC TCTACCCTGA CAATCGGTTC CTCAAGTGAA 
TCTGATTGCA AAGAACTGGA TGCATGTGTC AGCAATCCGT GTGGCAATGG CGGCGTCTGT 
AACAGACTGA TTGACGGTTA CACGTGCGTC TGTCTGTCGG GTTTCATCGG CGACAACTGT 
GAAGTGAACA TAGACGACTG CGCGAGAAAT CCCTGTCTAA ACAACGGAAC CTGCGTAGAT 
GGGATTGACA CTTACTCTTG CGTATGCAGC CAGGGTTATA CAGGTAACAC TTGTGAGGAA 
GACATTGACG ACTGTCAACA TCTACCGTGC CTCAATAACG GGGCATGTGA AGATCTAGTA 
GGCAGTTTTA TCTGTCACTG TCAACCGGGC TTTATGGGAG CCACGTGTGA AATTACAGTT 
GATGCTTGCT ACCATCATGA ATGTAAAAAC AGTGCAACGT GTGTTAGTCA GGATACGTCA 
TACGAGTGTG TCTGTCCAAA TGGCTTCACA GGAGATTTGT GTGAGACAAA CATAGATGAC 
TGTCGCGGAA ACCCTTGTCT TCACAACGGC GTGTGCGTTG ATGGTGTCAT GGACTTCACA 
TGTCTGTGCA CCATGGGCTA CACGGGTAGT TTGTGCGAGG TTGATGTGGA TCTTTGTGAC 
TCGGGACCTT GTCTAAACAG CGGTACTTGC GTAGACCTTG GAGACGACTT TGCTTGCCAG 
TGTGTCGAAG GACTGTCGGG GAAAACATGC GAGTCACCTA GAGATCCGTG TTCAGTTCAA 
CCGTGTCTCA ACGGTGGCAG CTGTACGGTG CAACCGCAAA CGGGTGACTA CGTCTGTAGC 
TGTGCACAAG GCTTCGCAGG CATACAATGT GAAATAGACA TCGACGAGTG CGCATCTGGA 
CCCTGTTCGA ATGACGCTAT TTGTTTAGAC GGGATAAATC AGTATTCCTG CGCCTGCACC 
AATGGCTTTG TTGGTCACAA CTGTGAAGAA GCAGTAGACC ACTGTGCTTC ATCCCCGTGC 
GGCAACAACT CCACCTGCCT CTCTGAACGA GATGGGTACC GCTGTATCTG TCCTCCTACA 
GCCACAGGAC CGACATGTGG TACTCCTGTA GATCTCTGTA CATCATCGCC ATGTGTAAAC 
AATGGGTCAT GTGTAGTAGA TGGCAACGGC TACACCTGTT ATTGCAGTCC TGATTTCACA 
GGACCCACGT GTGATACAGA AGAGGATAGT TGCGAGGATG ACCCTTGCGC AAACGGCGGG 
CTATGTACCG ATGGCTTTGG TACCTTCTCC TGCGCCTGCC CCGACGGCTA CACTGGCTTA 
ACGTGCGAAC AGGAGATCAA CGAATGCACC AGCAATCCGT GTCAAAACTC GGGCGTATGC 
GTCGACAAGG AAGCCGGATA CGATTGCTTG TGCCCCAAAG GTACCTCAGG CAACCATTGC 
GAAGAGAACT TTGACGACTG CCACAACGTA ACTTGCCTTA ATGGCGGTTC GTGTATTGAC 
CTTGTAAGCG CATTCCTCTG TAACTGCTCC CCAGGGTACG AAGGGAAATT TTGCGAGAGC 
GAAATCAACG AGTGTAACAT CTATCCCTGC AGAAACGCAA GATCCTGCCG GGATCTTCTG 
AACGACTATG AGTGTGAATG TGAACCAGGA TGGACGGGTA AGAACTGCGA GACGAATATC 
GATGACTGTT TCCCGAATCC TTGTGGCAAC GGCGGGGTAT GTCATGACCG TGTGAACAGC 
TACACATGCT CGTGCTTGGG CGGGTTTTCG GGGCTCCATT GTGAAGTCAA CATTGACGAA 
TGCATGGAGG TTCAATGCGA AAACAATGGT ACTTGCGTTG ACGGTGTAGG GACTGCGTCT 
TGCGTCTGCG GGTTAGGCTG GACAGGTACA AACTGTCAAG TGGAGATTGA CGAGTGTAGC 
TTGGAGCCTT GCATGAACGG AGGAGGGTGT GTTGACACCA TCGGGTCATT TGTCTGCAAC 
TGCGTCGACG GTTTTACGGG AAGGCTCTGT GAAGAAGACA TAAACGATTG CGACATACCA 
AGATGCATGA ACGGCGGAAC ATGCGTAGAT CAGGTAAATG GCATTATATA CGAGGCATTC 
ACTTGTAACG TGCGTGGTCC GGTGGTTAAG GTTGCCAGGT ACATGTGTAG GTGCCCTCCA 
GGCTATACCG GTACAAACTG TGAAGTCAAC ATCAACGAAT GTTCGTCACA GCCTTGCAGG 
AACGGCGGGC AATGTATTGA TGAATCCGAT GGTTTTATCT GCGTCTGTCC TCAGGGATTT 
TCGGGTCGAA CATGTGATGT CAACATAGAC GACTGCATTC CCAACATGTG CGAGAATGGA 
GCAACCTGTC TGGACGGGAT CGGTGACTAC ACCTGCATTT GCAAACTAGG ATTCAGTGGC 
CGTCGTTGTG AAGTAGATGT TGATGACTGT GCCAGTCGTC CTTGTGAGAA CACCGCAACT 
TGCCTGGATG GAGTCAATGA GTACACTTGC CTATGCCCAC CGGGATATTC TGGGGTGAGG 
TGTGAATATG CCCTTCCTAC AGACTTCGAC ATCCATGTGA CCTCATCAGA CAGCAATCAC 
TACTCAGAGG TTTCCAAGCA AAACATCCAC AGCATGTCTG CCATTGCCTG GTGGATGCGT 
GTAATGGACG CGCCAAACAA CTACGCCCCG GTAACAGTGG CCGTCACAAG GAATAACAGT 
GACGCGAGGG AACCTTTGTT CATTGTACAA AACCCAAACC GCATTGAGCT TGTTTTATCG 
GGTACTATTA TCCAGTCGTC TGTGAACTGT GCCGATGGCG GATGGCATCA CGTGGCCGTC 
TCATATGAGC CTGCGTCAGA GGTACTGGCT CTTTACGTCG ACGGAATGAT GGAAAACACA 
ACCCGCGTAC CCAGTCTCCA AAACTCGTCA CCAGCCGACG GTTTTGTCGT CTCTGTGGCC 
ACTGGGGTGA CCAATGCTAA CATCGGAGAC TCCGCGTTTC AAGGGGAGAT CAGCCAGCTG 
AACGCATGGG CACGAGAGTT GTCGCAGTCC GAGATTGTGG AACTCAGCAG TACCTGTCAG 
ACAGTGGCCG TAGGGGATGC TGTTGCGTGG TCTGACTTCG TCGATGCTAC ACAGACGGAT 
CCACAGTATG GTGCCATTTC GTCGCCGTCA GTTTGTGACT CTGAAGACCA ATGTGAAAGC 
TCTCCGTGTT TGAACGGTGG TACTTGCAGG GACGGATATC TTACCTTCAC CTGCACGTGT 
CCTCACAGCC ACGTGGGACA ACGTTGTGAG ACTCAACTCA ACCCCTGTGA TGGGAACCTG 
TGTCAGAACG GTGCCGCCTG CGTAGCTGAC GTGACAAGCA TGACATATAC CTGCAGCTGT 
CCTGAAGGGT GTTCAGGAGA TTTGTGTAAC GTGCTGTCAG TTAATGGTGG CTGGAGCGAG 
TGGAGCCAGT GGAGTGCTTG TACTGCCACT TGTGGCAACT CAACACGCTC CCGACAGCGT 
CTGTGCAACA ACCCTCCTCC GCTAGGCGGC GGGAACCCAT GTACCGGAGT TTCTCTGGAG 
ACAGAGGCGT GTGTTAACAT ACCAGACTGT ACTCGGTGCA CCGAGTTGCG GCATCCAGAA 
AATGGCTACC TGGTCTGCAG TCCCGACGGT GTAGACACTA AGTGTGAGGT TCACTGCAGA 
GCTGGGTTCA TGATGGTGGT CAACCCTGAT CTGTACGTAA ATCTTCGCTG TGGTCCCTCT 
ACATGGTACA CTTGGACGCA CCAAGACCTC ACAAATCTTC CAGCAAGGAT ACCTGGCTGT 
ACAAAGAAAG AGTTACCAAC CTCAGTAACT GTGGACTACG TCATTTGTTA CAAGAATGTC 
AGCGAAGACC CTTTCGCAAC CTTGTTTACA CAACAGATCC TGGGTCCCAG TGCTTCGTTC 
CTCAATGAAC AAACTTACGA ACATGCCTTT GATTCCGTTG TAACACGAAA TCTTTTGTCT 
CTTGACTGTA TTACCAGCCG TAACTGTCAC AAAGATGGTT CTGAAGTTCG CTTCGACAAC 
GTCACGACAG GCAACAATAC CAGATTGGAC GTTGTCGGTC ATATCTTCAT GTCTACGCAG 
GTTCCGCAGG TGTCAGATCC TGTTCTGACG CTTGCTCAGT CGTCAAAATA CAGCTTCCCA 
ATGGACGTGA TTTCGGGCGG TTTTGTTGCT GGAAACCCTG CGTTAACCTC CGTTGGAAAC 
CTAAGTCTTA TTGATGGCAT ATCGGGTAAG GCAATATCTC TAGACGGGCA GGCGCAATAC 
CTGAATGCAG GGCAATTTCC CGGAGAATGT CTTGGTTCGT CTGTGTATTG TCCGAATGGT 
TATACAATCA CAGCTTGGCT CCGCGTCAAC AGTGTGCCAT CATCACCATC ACATGACGTG 
TACTATCTTA GCAACGGCGG TCAGACTAGT TCTTCGAGGG GATTTGCCGT CGGATACAAA 
GGTGGCTTCT TCGCGCGGGT AAGCTTCGAC AGTCGCTATC GTTCGCTCGC CTTTTCGGTA 
GATTTGCACA CTTGGTTCAA CACAGCCCTG ACCTGGTCAC CCGGGGGAGA TCCCACACTT 
TACATCAATG GTACTCTCGT TAACGCGACG GTCACCGACG GTAGCCTGTC AGCCTTGACA 
TCAGATACAC GAACGCAGTT CCTCGTGGGT GCTCCCAACA ACATAGATAA CCCATCTTCG 
GGTCATCTTG GACATACCGA TGTCGACGAT GTCCTGTTCT GGGACCGGGT CCTGACGCAG 
GAAGAGATCC GAGATCTAAT GTCTGCCGAG CCAGTAGCTG TTGCAAGGAT CTGCCGAGGA 
CAACATCTGA CGCTAAGTTG CGAACCAGAA GAAGTCATCA CCATCCACTC AGCTACGTTC 
AAGGTATCAA CGCCTTCTGA GTGTGGAGCT TTGGACTCTT CACAGATAGA CAGCTGTGTG 
GATGATGCCG CTTTGGAATT TACCTTGAAC CTCTGTGACG GGCAGAGAAG CTGCCACCTC 
CATGCCACAG ATGAGGTCTT TGGGAATGAC TGTGTGGAGA CAAGAAATTC ATTCGAGGTT 
CTCTATACAT GCGACACGGG CCGCTTCAAT GACAAGGACA TAATCATGGA GAACATTGAA 
CAAGATTGGG AAACCACATT GAAAAGCCTG AGCCAAATGG TCGAAAATGG TGACTTTGAC 
CTGAACGTAC TGCATCAGGA CATTCCTGCA GACAAGAACA ACACAGAAGA GATAGAAATA 
CAACTAACCT GCACTGGCAA CATGGTTGGC ATGGGACTGT GTGACGCCTG TTTCCCTGGA 
TCGTTCTACA ATGGCCGGGA ATGCGTCCCG TGCCCACAGG GACAATTTCA GAGTCCGACA 
ACCCCAACCA AGTGCGAATC TTGTCCGCCG GGCTACACTA CTGTAGGATT CGGAAGCGTC 
GATGTGACTG CATGCTACGT GTACACGTGT ACATGTGACT TCAGCGTCCG GACGACTGAT 
CCAGATCTAC AGGGCAACGT ACGGTTCAAG TTCCGCTCGG TCAGGGCGTG TGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_92090 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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