BRAFLDRAFT_104468 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_104468
Entrez Gene ID	7212623
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002594501.1	XP_002594547.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_104468 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_104468 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb03223	XM_002594501.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_104468 ( XM_002594501.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002594547.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb03223
Clone ID Related Accession (Same CDS sequence)	XM_002594501.1
Accession Version	XM_002594501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101420). COMPLETENESS: incomplete on both ends.

ATGAGGATGT CCCAGGAGCT GAGTAAGACT TCAGGTGGCA TGCCACAATG TCAGATGCTT 
CTACAGTTCA TACGGCACGG AGTGGACAAC TTCCCCTGGT TGTTTGTACA CGAGCCGGTG 
TCCTCAGATG ACCCCTCCCC TGACACCTCC CAGGCCAGGG GAGACCTGAC GCTGACACAG 
GATGGGTACA GGGTGTCTAA AGAACCGAAG TGTGAGCCCC TCCACAGCAT CTCCCTGGAC 
ATCCTCACCT CGCTCATGCA CATGATCCGC ATCAAGGACG TCTTCATGTA CCAGGAGCTG 
GCCACCGAGT TTCTCGCTCT TATCGGAAGC TTGATTAGCA TAAGCAAGAC CGTGTTCAGA 
TTTGGCGAGA TCACGACGAC GGACCAGCCT ATCCGCTTGG AGTATTTCGT TCAGCAGGAC 
GACACAAGTG CTTCTGGGAG TCAGACCATG AAGGACGAAC CCATCAGCAT CAAGTATTTC 
GCTAATCTGT ACCTGCTGCA GATTGCACTG AGGGATGTTG CAGGCTTGGA GAGAAGCCTG 
GCAGGGTGCC TAAATGCAGT GTTCCAAGTA GATGAGAAGA CAAACTCACC TGTCCACTGT 
ATCCCCCCAC ATCATGTCTG TGCCACACTG GAGATGCTGT CTGTCTTGCT TTCTGCGAAT 
GGGTTGGATA ATCTACACAC CAAGGAGTTG CGGTCCTGCG TGTGTGGTTT ACTGTGCTAC 
ATCTACAGAC ATGTGCCCGA GGGTTATGAG GGGTGTGTGA GTCTGAGGCA ACACCACCTG 
CAACAGGCCG TCCTGGGGCT ACTGCAGCAC CTGGGGACAG CTGTGCACCT GGGGACAGCT 
GCTGGTTCTC AGCAGTATCT GGTCCCTTGC CTCTTTGAGG CCATCTCTGC AAAGATGCCA 
CCCATCAAAG GTGATGATCC TGCTAGAGGT CAGGTTTCAT CTGACCAGGA AAGTGCCAGC 
AGTGGGGATG AGAAGCTGGA GAAAAGGGCC AAGCTGTCTC GCAGGAGAAG CCTGCTTCCA 
ACTCAGAGCC AGAGGGCAAC AAGCAGCAAG CAGCAAAAAC AAACTGGAGA GTCCCAGTCA 
ACAGAGAAGC CAGAGGTTTA CCAACTGTTG GAGAAGAAGT TGCAAGAGTT GTGCCTTAAG 
TTACAGCAAG AGCCTACAAA GGAGACTGCG TTAAGTCTGT TGGAGGGTAT CAGGGTCGCT 
GTGGAAGTCT CAGTGAGGTG TGCGGCAAGT CGAGGCATTG TGAAGGAATG GCTTCCTGCC 
AGCTACCTGC AGAACCTGTG CAGTGTGTGG TTCAACACAT TAAAGCAGGC ACCAGCGTTG 
TTGTCAAAGA ACGACCTTAA GGAATGTTAC CTACTCGTGG TACAGTCAAT AGGTGCGGTG 
CTGTCTGTGT CAGATATATC TGCATTTGGA GGGGAGAACT TACGGTTCAT GACTATGGTG 
TTGTCTTTCC CATGGCTACT GGAGGATCAG AACTGGTTGG ACCTCAGGGT TTCTGGCTGG 
TGTTCTGCAA AAGAAGCAGC TGATCTGTGT GGTCAGTTTG CTGAGGCTAT TGGTGGTGAA 
GCTGAATCTC TATGTGTTCA CCTCTTGGCT CTTGTACCGA GAGAGGTGGC TCCTAAATGG 
AGAATACACA TCATGAAGGC TGCACTGAAT GACAGCCGTG TCCAAGTAAG GACCAGCCTG 
GTGAGATCCT TCCCCATCCT CCTGCACACC CTGGGCCACA ACTCTCACCA TCTGGTGTAT 
GATATACTCC AGAACAACTC CAGTGATGAC CATCTGTACA AGGTGACAAG GCTGGGCTGT 
TCTGTCTGCC AACAGAAAGG AATGGTTGAG TCCATCCCAG AGTTGTTCTC CCACATCCGA 
GTGTCAGCGG ACGTCCCTGC CTCCATGTCC CTGCTGCAGA ACTTCCTCTC TCCTGTGGAG 
GACTCATCCT ATGATGTCAG AATGGCATTC AGCAAACAGG TTGGCCACCT GGCGCGAGGG 
TGGTTGAGTG ACTCGTCCAG CCCCCTGGTG GCCAAGCTAA AATCTGCATA CCTGCGAGCA 
AACAGCAGTT CCAACAGTAT TCCTCTTCAG GAGACAGTGG TCCTTACTAT TGGGCAGTTG 
GGCAGGATAG CTGAAGGTGA CTTGTTTGGT GTAGTGTTGA TAAACCTGCT GATCAGTCTG 
GTGTCCCAGT CCCCTGTGCT GGCAGCTCTG GCCTACAGTG AGATTCATGA CATTGCAAAG 
GTCAGAAAGA CAAACCTCAC CTCCCTCCTC ACCAAACAGA GGCCCATCAT TTGTAAGTGG 
TTGTCTGAGG TTCTCCACGA CTCCCAGGGT TCTGGAGGTA GTTCTACATG TAGACTGAAA 
AACCCTGCTG GAATTTTGAG TGAGGTTGCC AGTGTCTTTG GATGTGAAGA TGTCAGAAAA 
TTTTTGTTGA TGTGTCTCCA GACTCTCCTG CCACAGTTGG TACACAAGGC CTGTCCTGAA 
ACATCTGCCA TCATCAGACT CATTGCCAAG GAGCTGGAAA TGAATCGACA AGACCTGCTG 
ATCAACAACT TCAAGTACAT CTTCCGCTAC CTGGCCTGTA ACTGTAAGAC AGAAGAGAAG 
GAAAGAGCGC TTGAGTTTGT ACAGAATGAG ACCCAGATAG AGCTGAGGAG CCTCCTGAGG 
TCAGACTACC AGGGGCTCCA CAACGAGCTG CTGCTCTACA TCAGCTCCCA CTACAGCCAG 
GTCTTCGCTG GGCTGGCCAC CGTGGCTGCT TCCACCCGCG AAGGGAAGGG CAAGGGCAAG 
GTCCCCAGGG AGATCACTAC AGCACAGGAA ATGGGTTACC AGGGAGATCA CTACAGCACA 
GGAGATGGTG GGTACATAAA GGGTTACCAG GGAGATCACT ACAGCACAGG AGATGGTGGG 
TACATAAAGG GTTACCAGGG AGATCACTAC AGCACAGGAG ATGGTGGGTA CATAAAGGGT 
TACCAGGGAG ATCACTACAG CACAGGAGAT GGTGGGTACA TAAAGGGTTA CCAGGGAGAT 
CACTACAGCA CAGGAGATGG TGGGTACATA AAGGGTTACC AGGGAGATCA CTACAGCACA 
GGAGATGGTG GGTACATAAG GGTTACCAGG GAGATCACTA CAGCACAGGA GATGGCTGAG 
TTTCTACAGC CATGTTTGCT GGGTATCCTG GCTTTTTTTG ACTCCCAGCT GCTCACCACC 
TCAATACCAA TGGAGGATAA GAAGTTGGCG CTGGAGAGCC TGATCACCTT GATGAAACTG 
ATGGGACCAA AACACATCAC AGCTGTGAGA GTCAAGGTCA TGACTACACT AAGGATTTGT 
CTCCGGTTTA AAGTTAAGGG GTTTCCCGAA CTGAGCTGCA GGGCCTGGGA CTGCTTTGTA 
CACAGTGTGG AGTTGTCGTG TCTGGGCCCC ATGTTGAGTC AGATCGTGGT GACCCTCCTA 
CCCTTACTAC AGACACAGGC AAAGCCTGTC GCAGGCGTCT TCAGCTTCCT CATACAGGAC 
AATAGGGAAG CTTTGCAGGA CCACTTCCAT GAACTGTACT TCATGCCAGA GCTGCCGGAA 
CTTTCCAAGA TCAACGCTGT GCTCAAACAA CATATAGAAA CACCATCCAG CCAATCTGAC 
CTGAAGAGCC AGCTGAAGCA CTCCCTGAAG GGGCTGGGGC ACGAGAGCCT GGATGTGAGA 
CTACACGCCC TCTCCAAGCT GAAACAGCAG CTGCATGCTA ACCAGCTGTT ATCTTCCTGT 
CGTGAGTCTG ACAAGACAGC GAGAAGCCTT GTGGGGGAGT GTCTGGGAGA ACTGGGTGCT 
ATCGATCCTG GAAGATTGGA TCTTAAGGCC CATGGACAAA AGGAAGAACT ATCAAAATTC 
CAGGCTGGGG TTGATGATAT CAACTTTGCC CATGACCTGA TCATGGAGCT GGCTCGAGCC 
TTTCTAGCCG CTACCGACAC AAGGTCACAG GACTGCTCTG CATTTTCCAT ACAAGAGATA 
CTCCAGACAT ACGAGTGTCG GGAGGGAACA AAAGACACCC TGGGGCGGAG GCTGTGGAGG 
GGGTTCCCAG AACACATTCA GGAGATTCTT TTACCTCATT TACACTCCAA GTACATCGTG 
TTCTCCACTG CTACGTGGAC CAACATGAAG AAGCCCATCT ACCGTAGTAA CAAAGGGAGC 
ACCTTTCAGG ACTGGGTCTA CAACTGGACA GGCTACCTTA TCACTAAGGT AAAGCAGGAC 
ATGGCGTCCC GTGTGTTCCG TGCCTGCAGC ACTGTCACCA AGTACAATGA AACTACAGCT 
CTGTTCCTGC TGCCCCATGT GCTGCTGCAT GCACTGTTGG ATGGCAGCAC TGAGGAGAAA 
CAGGAGGCAT GTAACGAAAT CCTGGCAGTT CTGAAGTACG CTGAAAAGAT GGAGGAGAAG 
GGGGCGCAGG ATTTCTCCCA CATGGCAGCG CAGACCGTGT TCTCGGTGCT GGACCATCTC 
ACGCGCTGGT GCCGCCACAG ACAGCTCACC CTGTCAGTAC AGAGGGACGA TGGGAAAAAG 
GGCAACAGGT CATCAGATAG GGTTCTCCTG CGAGACGACA GCCCGTACAA CTCCGTGCAG 
GGTTTCCTGG ACCTGATCCC TCAGGACGTG CTGGCTGTGG CATCCTTCCA CTGCAGGGCC 
TACACGCGGG CACTCATGCA CTTCGAGTCC TACCTGTCCG CCAGTAAACC AGACATCGAA 
CCACAGTTGT GCTTGTTACA GAAGCTGTAC GTAGCCATGG ATGAACCAGA TGGGGTAGCT 
GGGGTGATGG CCATACGTAA GAGTGAGCCT GATATAAAGG AACAGATCCT TCAGCATGAG 
AGCATAGGGG AGCTGCGTGA TGCGTCTGCC TGCTACGAGC GAGCGCTGCA GACGGAGGCC 
AGCACCGTGC CTCACCACCA GGGCCTGCTG CGCTGTCTGC TGGGTCTGGG GCAGCTCACC 
ACGGCCATGG TGCACGTCAA CGGGGTTCTC GCAGACAAGC CAGAGTGGAC CAAGGACCTG 
AATGCCTTCA GAGTGGAGGC CTCATGGCAG CTGGGTAACT GGGACGGACT GGAGAACTAT 
CTGAAAGTCT ACAATGGAAA GTCTGAGAGC AAGGGTTCCA GTAACTGGAG TGTTGGGCTG 
GGAAAGATTC TCCTGGCTGC AAAACACAGG GATGAGGAAG ACTTCTGGCA CCAGCTGCAG 
ATCGTGCGGA ATGACCAGAT GGGCCCGCTG TCTGCAGCCA GCATGGAGAG TGGGTCGTAC 
CAACGGGGAT ACGACTACAT CGTCAGACTG CACATGTTGT GTGAGCTGCA GCACGGAGTG 
AAGGTACTGC TGAGGCGGGA GGATGCTGGG GAGCAGGAGG ACGGGAGTCT AGACCTGGAG 
GCGCGGGCTC GCATCACGCA GTCGTCCTTC CGCACGCGCG AACCTCTCTA CAGCCTCAGG 
AGGTGTATCA TCAACATGGC TGGCAGTCCT GATACAGTAG GTAAAGAGCT GGGACACTGC 
TGGCTGCAGA GTGCTAAGAT CGCCCGTAAG GCGGGTCATC TCCAGACTGC CTACAGTTCC 
CTGCTGAACG CCAGCACCTA CAGTCTACCT GAACTACTGG TGGAGAAGGC CAAGTGGTTG 
TGGCATCAGA ATGACCAGCA CCAGGCCCTG ATCACCCTGC AGAAGGGTGT GGCTGAACAC 
TTCTCTGACA CTGCCTACAT GAACTGTGGC AGCTCTGAGG CAGCCAACGC TAAAAAACAC 
ACACATGCCA AGGCCCTGCT TCTAGTTGGT AGATTGATGG AGGATACTGC TATGTACGAT 
AGCAACCAAG TCATGAAGCA ATACAAGAGT GTTGTGGAGG TTTACCAAGA GTGGGAAGAT 
GGACACTTCT ACCTGGCTAA GTACTATGAC CGCATCATGA CTACCATGCG TGAAACACCA 
GTCAAGGCAG GAGAAATCAT CCTGTACGTG GTGACATACT TCGGGCGCTC CCTGCAGTAC 
GGTAACCAGC ACATCTACCA GTCCATGCCC AGGCTACTGG CCCTGTGGCT CGACTACGGG 
GCAAAGGTGT CAGATTTGGA GAAAGATGTG TTTGAAGTAA GATGGCATTT CTTTTTTTCA 
GCTGGAAGGG CAGAGAGGTC AAACATGAGG GTTCTGCTCC CGAAGCTTAA CGAGATTATT 
GCAAATCTCA CCAGGAAGTT AGCCCCATAC CAGTTCCTGA CGTCCTTCTC ACAGCTCATC 
TCGCGCATCT GTCACTCACA CGCAGAGGTC CTCACCAGAC TGGAGGATAT TATTGCCATG 
CTGTTGGTGA CCTTCCCACA GCAGTGTATG TGGCTGATGA TGGCTGTGTC CAAGTCAACA 
TCTCTGATAC GGAAGAAGCG CTGCCAGGAC ATCTTCAAGA AAGCCAAGTC CATGAACAGT 
GAGCTCAACC AGTTCATACA GGATGCCACC AGACTGGCTG ACAAGCTCCT GGAGTTGTGC 
AACAAGCCAG TAGATCCAGG TGTGCAACAC TTCAGCCTCA GTCTGCACTT CAAGTCACTC 
AAGAGACTGC TGGAGGACAG GAAGTTCAGC CCGATCCTGA TGCCCCTCCA GTCAGCCCTG 
ACCGTCACCC TGCCCAGCGG ACCTGGCCCC CACGCCAACC ACGAGCCCTT CCCTGCTGCA 
CTGGTCTACA TTGCTGGGTT TGAAGACACT GTAGAGCTCT TGCCATCTTT GCAACGTCCC 
AAGAAGTTCT CGTTTCGTGG TACAGATGGT CATTCCTACA TCATGATGTG TAAACCCAAG 
GTGGAGGTTT TGAGTTCTGC AGCCAAGCCA AAGAAAATAG CAGTCAGGGG TTCAGACGGG 
AAGGCATACT TCCTCATGTG CAAACCTCAC GATGACCTGC GGAAGGACTG TCGGCTGATG 
GAGTTCAATG CTATTGTCAA TAAATGCCTG AGGAAAGACC CAGAGTCTCG GAGAAGGCAG 
CTGTACATCA GGACCTATTC GGTGGTTCCC CTGAATGAAG AATGCGGGCT GATAGAGTGG 
GTAAACAACA CCCACCCTCT ACGTCAGGTG CTCATCAAGA TCTACAAGGA AAGACTGCAC 
ATGTTTCAGA ACACCATCCT ACCCAAGCAC CCTCCTGTGT TCAGGGAGTG GTTCCTGAAA 
GTGTTCCCTG ATCCAACCTC ATGGTACCAG GCCCGTCTGA ACTACTGCCG CACCACAGCA 
GTCATGTCCA TGGTGGGGTA CATCCTGGGG CTGGGGGATC GACATGGAGA AAACATCCTG 
TTCGACTCCA CCAATGGGGA CTGTGTGCAT GTGGACTTCA ACTGTCTCTT CAACAAGGGA 
GAAACATTTG ACTGGCCAGA GTTGGTGCCC TTCCGTCTAA ACACAACATG GTCAACGCAA 
TGTGTGCTGA GGTCCTTTAT CTTCGACCCC TTGGTGGAAT GGTCCCGAGT GAACAAGAGC 
ACCAGGTCTG TGGTTCCTGA GTCAGGAGAG ATCAGCAATG AGAAGGCTAT GACACACGTG 
AGGGACATAG ACCAGCGGCT TCAGGGGGTG TTCAAGAAGA TGCGAGGGCT TCCACTGTCC 
ATCGAAGGCC ATGTTCACCA TCTCATACAG ATTAATGGTA CATTCACCTT AAAGGCCTAC 
AGTAGGAAAC CACTTGAGCT GGACTACAAG GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002594547.1
CDS	1..7596
Translation	MRMSQELSKTSGGMPQCQMLLQFIRHGVDNFPWLFVHEPVSSDDPSPDTSQARGDLTLTQDGYRVSKEPKCEPLHSISLDILTSLMHMIRIKDVFMYQELATEFLALIGSLISISKTVFRFGEITTTDQPIRLEYFVQQDDTSASGSQTMKDEPISIKYFANLYLLQIALRDVAGLERSLAGCLNAVFQVDEKTNSPVHCIPPHHVCATLEMLSVLLSANGLDNLHTKELRSCVCGLLCYIYRHVPEGYEGCVSLRQHHLQQAVLGLLQHLGTAVHLGTAAGSQQYLVPCLFEAISAKMPPIKGDDPARGQVSSDQESASSGDEKLEKRAKLSRRRSLLPTQSQRATSSKQQKQTGESQSTEKPEVYQLLEKKLQELCLKLQQEPTKETALSLLEGIRVAVEVSVRCAASRGIVKEWLPASYLQNLCSVWFNTLKQAPALLSKNDLKECYLLVVQSIGAVLSVSDISAFGGENLRFMTMVLSFPWLLEDQNWLDLRVSGWCSAKEAADLCGQFAEAIGGEAESLCVHLLALVPREVAPKWRIHIMKAALNDSRVQVRTSLVRSFPILLHTLGHNSHHLVYDILQNNSSDDHLYKVTRLGCSVCQQKGMVESIPELFSHIRVSADVPASMSLLQNFLSPVEDSSYDVRMAFSKQVGHLARGWLSDSSSPLVAKLKSAYLRANSSSNSIPLQETVVLTIGQLGRIAEGDLFGVVLINLLISLVSQSPVLAALAYSEIHDIAKVRKTNLTSLLTKQRPIICKWLSEVLHDSQGSGGSSTCRLKNPAGILSEVASVFGCEDVRKFLLMCLQTLLPQLVHKACPETSAIIRLIAKELEMNRQDLLINNFKYIFRYLACNCKTEEKERALEFVQNETQIELRSLLRSDYQGLHNELLLYISSHYSQVFAGLATVAASTREGKGKGKVPREITTAQEMGYQGDHYSTGDGGYIKGYQGDHYSTGDGGYIKGYQGDHYSTGDGGYIKGYQGDHYSTGDGGYIKGYQGDHYSTGDGGYIKGYQGDHYSTGDGGYIRVTREITTAQEMAEFLQPCLLGILAFFDSQLLTTSIPMEDKKLALESLITLMKLMGPKHITAVRVKVMTTLRICLRFKVKGFPELSCRAWDCFVHSVELSCLGPMLSQIVVTLLPLLQTQAKPVAGVFSFLIQDNREALQDHFHELYFMPELPELSKINAVLKQHIETPSSQSDLKSQLKHSLKGLGHESLDVRLHALSKLKQQLHANQLLSSCRESDKTARSLVGECLGELGAIDPGRLDLKAHGQKEELSKFQAGVDDINFAHDLIMELARAFLAATDTRSQDCSAFSIQEILQTYECREGTKDTLGRRLWRGFPEHIQEILLPHLHSKYIVFSTATWTNMKKPIYRSNKGSTFQDWVYNWTGYLITKVKQDMASRVFRACSTVTKYNETTALFLLPHVLLHALLDGSTEEKQEACNEILAVLKYAEKMEEKGAQDFSHMAAQTVFSVLDHLTRWCRHRQLTLSVQRDDGKKGNRSSDRVLLRDDSPYNSVQGFLDLIPQDVLAVASFHCRAYTRALMHFESYLSASKPDIEPQLCLLQKLYVAMDEPDGVAGVMAIRKSEPDIKEQILQHESIGELRDASACYERALQTEASTVPHHQGLLRCLLGLGQLTTAMVHVNGVLADKPEWTKDLNAFRVEASWQLGNWDGLENYLKVYNGKSESKGSSNWSVGLGKILLAAKHRDEEDFWHQLQIVRNDQMGPLSAASMESGSYQRGYDYIVRLHMLCELQHGVKVLLRREDAGEQEDGSLDLEARARITQSSFRTREPLYSLRRCIINMAGSPDTVGKELGHCWLQSAKIARKAGHLQTAYSSLLNASTYSLPELLVEKAKWLWHQNDQHQALITLQKGVAEHFSDTAYMNCGSSEAANAKKHTHAKALLLVGRLMEDTAMYDSNQVMKQYKSVVEVYQEWEDGHFYLAKYYDRIMTTMRETPVKAGEIILYVVTYFGRSLQYGNQHIYQSMPRLLALWLDYGAKVSDLEKDVFEVRWHFFFSAGRAERSNMRVLLPKLNEIIANLTRKLAPYQFLTSFSQLISRICHSHAEVLTRLEDIIAMLLVTFPQQCMWLMMAVSKSTSLIRKKRCQDIFKKAKSMNSELNQFIQDATRLADKLLELCNKPVDPGVQHFSLSLHFKSLKRLLEDRKFSPILMPLQSALTVTLPSGPGPHANHEPFPAALVYIAGFEDTVELLPSLQRPKKFSFRGTDGHSYIMMCKPKVEVLSSAAKPKKIAVRGSDGKAYFLMCKPHDDLRKDCRLMEFNAIVNKCLRKDPESRRRQLYIRTYSVVPLNEECGLIEWVNNTHPLRQVLIKIYKERLHMFQNTILPKHPPVFREWFLKVFPDPTSWYQARLNYCRTTAVMSMVGYILGLGDRHGENILFDSTNGDCVHVDFNCLFNKGETFDWPELVPFRLNTTWSTQCVLRSFIFDPLVEWSRVNKSTRSVVPESGEISNEKAMTHVRDIDQRLQGVFKKMRGLPLSIEGHVHHLIQINGTFTLKAYSRKPLELDYKG

Target ORF information:

RefSeq Version	XM_002594501.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002594501.1

ORF Insert Sequence:

ATGAGGATGT CCCAGGAGCT GAGTAAGACT TCAGGTGGCA TGCCACAATG TCAGATGCTT 
CTACAGTTCA TACGGCACGG AGTGGACAAC TTCCCCTGGT TGTTTGTACA CGAGCCGGTG 
TCCTCAGATG ACCCCTCCCC TGACACCTCC CAGGCCAGGG GAGACCTGAC GCTGACACAG 
GATGGGTACA GGGTGTCTAA AGAACCGAAG TGTGAGCCCC TCCACAGCAT CTCCCTGGAC 
ATCCTCACCT CGCTCATGCA CATGATCCGC ATCAAGGACG TCTTCATGTA CCAGGAGCTG 
GCCACCGAGT TTCTCGCTCT TATCGGAAGC TTGATTAGCA TAAGCAAGAC CGTGTTCAGA 
TTTGGCGAGA TCACGACGAC GGACCAGCCT ATCCGCTTGG AGTATTTCGT TCAGCAGGAC 
GACACAAGTG CTTCTGGGAG TCAGACCATG AAGGACGAAC CCATCAGCAT CAAGTATTTC 
GCTAATCTGT ACCTGCTGCA GATTGCACTG AGGGATGTTG CAGGCTTGGA GAGAAGCCTG 
GCAGGGTGCC TAAATGCAGT GTTCCAAGTA GATGAGAAGA CAAACTCACC TGTCCACTGT 
ATCCCCCCAC ATCATGTCTG TGCCACACTG GAGATGCTGT CTGTCTTGCT TTCTGCGAAT 
GGGTTGGATA ATCTACACAC CAAGGAGTTG CGGTCCTGCG TGTGTGGTTT ACTGTGCTAC 
ATCTACAGAC ATGTGCCCGA GGGTTATGAG GGGTGTGTGA GTCTGAGGCA ACACCACCTG 
CAACAGGCCG TCCTGGGGCT ACTGCAGCAC CTGGGGACAG CTGTGCACCT GGGGACAGCT 
GCTGGTTCTC AGCAGTATCT GGTCCCTTGC CTCTTTGAGG CCATCTCTGC AAAGATGCCA 
CCCATCAAAG GTGATGATCC TGCTAGAGGT CAGGTTTCAT CTGACCAGGA AAGTGCCAGC 
AGTGGGGATG AGAAGCTGGA GAAAAGGGCC AAGCTGTCTC GCAGGAGAAG CCTGCTTCCA 
ACTCAGAGCC AGAGGGCAAC AAGCAGCAAG CAGCAAAAAC AAACTGGAGA GTCCCAGTCA 
ACAGAGAAGC CAGAGGTTTA CCAACTGTTG GAGAAGAAGT TGCAAGAGTT GTGCCTTAAG 
TTACAGCAAG AGCCTACAAA GGAGACTGCG TTAAGTCTGT TGGAGGGTAT CAGGGTCGCT 
GTGGAAGTCT CAGTGAGGTG TGCGGCAAGT CGAGGCATTG TGAAGGAATG GCTTCCTGCC 
AGCTACCTGC AGAACCTGTG CAGTGTGTGG TTCAACACAT TAAAGCAGGC ACCAGCGTTG 
TTGTCAAAGA ACGACCTTAA GGAATGTTAC CTACTCGTGG TACAGTCAAT AGGTGCGGTG 
CTGTCTGTGT CAGATATATC TGCATTTGGA GGGGAGAACT TACGGTTCAT GACTATGGTG 
TTGTCTTTCC CATGGCTACT GGAGGATCAG AACTGGTTGG ACCTCAGGGT TTCTGGCTGG 
TGTTCTGCAA AAGAAGCAGC TGATCTGTGT GGTCAGTTTG CTGAGGCTAT TGGTGGTGAA 
GCTGAATCTC TATGTGTTCA CCTCTTGGCT CTTGTACCGA GAGAGGTGGC TCCTAAATGG 
AGAATACACA TCATGAAGGC TGCACTGAAT GACAGCCGTG TCCAAGTAAG GACCAGCCTG 
GTGAGATCCT TCCCCATCCT CCTGCACACC CTGGGCCACA ACTCTCACCA TCTGGTGTAT 
GATATACTCC AGAACAACTC CAGTGATGAC CATCTGTACA AGGTGACAAG GCTGGGCTGT 
TCTGTCTGCC AACAGAAAGG AATGGTTGAG TCCATCCCAG AGTTGTTCTC CCACATCCGA 
GTGTCAGCGG ACGTCCCTGC CTCCATGTCC CTGCTGCAGA ACTTCCTCTC TCCTGTGGAG 
GACTCATCCT ATGATGTCAG AATGGCATTC AGCAAACAGG TTGGCCACCT GGCGCGAGGG 
TGGTTGAGTG ACTCGTCCAG CCCCCTGGTG GCCAAGCTAA AATCTGCATA CCTGCGAGCA 
AACAGCAGTT CCAACAGTAT TCCTCTTCAG GAGACAGTGG TCCTTACTAT TGGGCAGTTG 
GGCAGGATAG CTGAAGGTGA CTTGTTTGGT GTAGTGTTGA TAAACCTGCT GATCAGTCTG 
GTGTCCCAGT CCCCTGTGCT GGCAGCTCTG GCCTACAGTG AGATTCATGA CATTGCAAAG 
GTCAGAAAGA CAAACCTCAC CTCCCTCCTC ACCAAACAGA GGCCCATCAT TTGTAAGTGG 
TTGTCTGAGG TTCTCCACGA CTCCCAGGGT TCTGGAGGTA GTTCTACATG TAGACTGAAA 
AACCCTGCTG GAATTTTGAG TGAGGTTGCC AGTGTCTTTG GATGTGAAGA TGTCAGAAAA 
TTTTTGTTGA TGTGTCTCCA GACTCTCCTG CCACAGTTGG TACACAAGGC CTGTCCTGAA 
ACATCTGCCA TCATCAGACT CATTGCCAAG GAGCTGGAAA TGAATCGACA AGACCTGCTG 
ATCAACAACT TCAAGTACAT CTTCCGCTAC CTGGCCTGTA ACTGTAAGAC AGAAGAGAAG 
GAAAGAGCGC TTGAGTTTGT ACAGAATGAG ACCCAGATAG AGCTGAGGAG CCTCCTGAGG 
TCAGACTACC AGGGGCTCCA CAACGAGCTG CTGCTCTACA TCAGCTCCCA CTACAGCCAG 
GTCTTCGCTG GGCTGGCCAC CGTGGCTGCT TCCACCCGCG AAGGGAAGGG CAAGGGCAAG 
GTCCCCAGGG AGATCACTAC AGCACAGGAA ATGGGTTACC AGGGAGATCA CTACAGCACA 
GGAGATGGTG GGTACATAAA GGGTTACCAG GGAGATCACT ACAGCACAGG AGATGGTGGG 
TACATAAAGG GTTACCAGGG AGATCACTAC AGCACAGGAG ATGGTGGGTA CATAAAGGGT 
TACCAGGGAG ATCACTACAG CACAGGAGAT GGTGGGTACA TAAAGGGTTA CCAGGGAGAT 
CACTACAGCA CAGGAGATGG TGGGTACATA AAGGGTTACC AGGGAGATCA CTACAGCACA 
GGAGATGGTG GGTACATAAG GGTTACCAGG GAGATCACTA CAGCACAGGA GATGGCTGAG 
TTTCTACAGC CATGTTTGCT GGGTATCCTG GCTTTTTTTG ACTCCCAGCT GCTCACCACC 
TCAATACCAA TGGAGGATAA GAAGTTGGCG CTGGAGAGCC TGATCACCTT GATGAAACTG 
ATGGGACCAA AACACATCAC AGCTGTGAGA GTCAAGGTCA TGACTACACT AAGGATTTGT 
CTCCGGTTTA AAGTTAAGGG GTTTCCCGAA CTGAGCTGCA GGGCCTGGGA CTGCTTTGTA 
CACAGTGTGG AGTTGTCGTG TCTGGGCCCC ATGTTGAGTC AGATCGTGGT GACCCTCCTA 
CCCTTACTAC AGACACAGGC AAAGCCTGTC GCAGGCGTCT TCAGCTTCCT CATACAGGAC 
AATAGGGAAG CTTTGCAGGA CCACTTCCAT GAACTGTACT TCATGCCAGA GCTGCCGGAA 
CTTTCCAAGA TCAACGCTGT GCTCAAACAA CATATAGAAA CACCATCCAG CCAATCTGAC 
CTGAAGAGCC AGCTGAAGCA CTCCCTGAAG GGGCTGGGGC ACGAGAGCCT GGATGTGAGA 
CTACACGCCC TCTCCAAGCT GAAACAGCAG CTGCATGCTA ACCAGCTGTT ATCTTCCTGT 
CGTGAGTCTG ACAAGACAGC GAGAAGCCTT GTGGGGGAGT GTCTGGGAGA ACTGGGTGCT 
ATCGATCCTG GAAGATTGGA TCTTAAGGCC CATGGACAAA AGGAAGAACT ATCAAAATTC 
CAGGCTGGGG TTGATGATAT CAACTTTGCC CATGACCTGA TCATGGAGCT GGCTCGAGCC 
TTTCTAGCCG CTACCGACAC AAGGTCACAG GACTGCTCTG CATTTTCCAT ACAAGAGATA 
CTCCAGACAT ACGAGTGTCG GGAGGGAACA AAAGACACCC TGGGGCGGAG GCTGTGGAGG 
GGGTTCCCAG AACACATTCA GGAGATTCTT TTACCTCATT TACACTCCAA GTACATCGTG 
TTCTCCACTG CTACGTGGAC CAACATGAAG AAGCCCATCT ACCGTAGTAA CAAAGGGAGC 
ACCTTTCAGG ACTGGGTCTA CAACTGGACA GGCTACCTTA TCACTAAGGT AAAGCAGGAC 
ATGGCGTCCC GTGTGTTCCG TGCCTGCAGC ACTGTCACCA AGTACAATGA AACTACAGCT 
CTGTTCCTGC TGCCCCATGT GCTGCTGCAT GCACTGTTGG ATGGCAGCAC TGAGGAGAAA 
CAGGAGGCAT GTAACGAAAT CCTGGCAGTT CTGAAGTACG CTGAAAAGAT GGAGGAGAAG 
GGGGCGCAGG ATTTCTCCCA CATGGCAGCG CAGACCGTGT TCTCGGTGCT GGACCATCTC 
ACGCGCTGGT GCCGCCACAG ACAGCTCACC CTGTCAGTAC AGAGGGACGA TGGGAAAAAG 
GGCAACAGGT CATCAGATAG GGTTCTCCTG CGAGACGACA GCCCGTACAA CTCCGTGCAG 
GGTTTCCTGG ACCTGATCCC TCAGGACGTG CTGGCTGTGG CATCCTTCCA CTGCAGGGCC 
TACACGCGGG CACTCATGCA CTTCGAGTCC TACCTGTCCG CCAGTAAACC AGACATCGAA 
CCACAGTTGT GCTTGTTACA GAAGCTGTAC GTAGCCATGG ATGAACCAGA TGGGGTAGCT 
GGGGTGATGG CCATACGTAA GAGTGAGCCT GATATAAAGG AACAGATCCT TCAGCATGAG 
AGCATAGGGG AGCTGCGTGA TGCGTCTGCC TGCTACGAGC GAGCGCTGCA GACGGAGGCC 
AGCACCGTGC CTCACCACCA GGGCCTGCTG CGCTGTCTGC TGGGTCTGGG GCAGCTCACC 
ACGGCCATGG TGCACGTCAA CGGGGTTCTC GCAGACAAGC CAGAGTGGAC CAAGGACCTG 
AATGCCTTCA GAGTGGAGGC CTCATGGCAG CTGGGTAACT GGGACGGACT GGAGAACTAT 
CTGAAAGTCT ACAATGGAAA GTCTGAGAGC AAGGGTTCCA GTAACTGGAG TGTTGGGCTG 
GGAAAGATTC TCCTGGCTGC AAAACACAGG GATGAGGAAG ACTTCTGGCA CCAGCTGCAG 
ATCGTGCGGA ATGACCAGAT GGGCCCGCTG TCTGCAGCCA GCATGGAGAG TGGGTCGTAC 
CAACGGGGAT ACGACTACAT CGTCAGACTG CACATGTTGT GTGAGCTGCA GCACGGAGTG 
AAGGTACTGC TGAGGCGGGA GGATGCTGGG GAGCAGGAGG ACGGGAGTCT AGACCTGGAG 
GCGCGGGCTC GCATCACGCA GTCGTCCTTC CGCACGCGCG AACCTCTCTA CAGCCTCAGG 
AGGTGTATCA TCAACATGGC TGGCAGTCCT GATACAGTAG GTAAAGAGCT GGGACACTGC 
TGGCTGCAGA GTGCTAAGAT CGCCCGTAAG GCGGGTCATC TCCAGACTGC CTACAGTTCC 
CTGCTGAACG CCAGCACCTA CAGTCTACCT GAACTACTGG TGGAGAAGGC CAAGTGGTTG 
TGGCATCAGA ATGACCAGCA CCAGGCCCTG ATCACCCTGC AGAAGGGTGT GGCTGAACAC 
TTCTCTGACA CTGCCTACAT GAACTGTGGC AGCTCTGAGG CAGCCAACGC TAAAAAACAC 
ACACATGCCA AGGCCCTGCT TCTAGTTGGT AGATTGATGG AGGATACTGC TATGTACGAT 
AGCAACCAAG TCATGAAGCA ATACAAGAGT GTTGTGGAGG TTTACCAAGA GTGGGAAGAT 
GGACACTTCT ACCTGGCTAA GTACTATGAC CGCATCATGA CTACCATGCG TGAAACACCA 
GTCAAGGCAG GAGAAATCAT CCTGTACGTG GTGACATACT TCGGGCGCTC CCTGCAGTAC 
GGTAACCAGC ACATCTACCA GTCCATGCCC AGGCTACTGG CCCTGTGGCT CGACTACGGG 
GCAAAGGTGT CAGATTTGGA GAAAGATGTG TTTGAAGTAA GATGGCATTT CTTTTTTTCA 
GCTGGAAGGG CAGAGAGGTC AAACATGAGG GTTCTGCTCC CGAAGCTTAA CGAGATTATT 
GCAAATCTCA CCAGGAAGTT AGCCCCATAC CAGTTCCTGA CGTCCTTCTC ACAGCTCATC 
TCGCGCATCT GTCACTCACA CGCAGAGGTC CTCACCAGAC TGGAGGATAT TATTGCCATG 
CTGTTGGTGA CCTTCCCACA GCAGTGTATG TGGCTGATGA TGGCTGTGTC CAAGTCAACA 
TCTCTGATAC GGAAGAAGCG CTGCCAGGAC ATCTTCAAGA AAGCCAAGTC CATGAACAGT 
GAGCTCAACC AGTTCATACA GGATGCCACC AGACTGGCTG ACAAGCTCCT GGAGTTGTGC 
AACAAGCCAG TAGATCCAGG TGTGCAACAC TTCAGCCTCA GTCTGCACTT CAAGTCACTC 
AAGAGACTGC TGGAGGACAG GAAGTTCAGC CCGATCCTGA TGCCCCTCCA GTCAGCCCTG 
ACCGTCACCC TGCCCAGCGG ACCTGGCCCC CACGCCAACC ACGAGCCCTT CCCTGCTGCA 
CTGGTCTACA TTGCTGGGTT TGAAGACACT GTAGAGCTCT TGCCATCTTT GCAACGTCCC 
AAGAAGTTCT CGTTTCGTGG TACAGATGGT CATTCCTACA TCATGATGTG TAAACCCAAG 
GTGGAGGTTT TGAGTTCTGC AGCCAAGCCA AAGAAAATAG CAGTCAGGGG TTCAGACGGG 
AAGGCATACT TCCTCATGTG CAAACCTCAC GATGACCTGC GGAAGGACTG TCGGCTGATG 
GAGTTCAATG CTATTGTCAA TAAATGCCTG AGGAAAGACC CAGAGTCTCG GAGAAGGCAG 
CTGTACATCA GGACCTATTC GGTGGTTCCC CTGAATGAAG AATGCGGGCT GATAGAGTGG 
GTAAACAACA CCCACCCTCT ACGTCAGGTG CTCATCAAGA TCTACAAGGA AAGACTGCAC 
ATGTTTCAGA ACACCATCCT ACCCAAGCAC CCTCCTGTGT TCAGGGAGTG GTTCCTGAAA 
GTGTTCCCTG ATCCAACCTC ATGGTACCAG GCCCGTCTGA ACTACTGCCG CACCACAGCA 
GTCATGTCCA TGGTGGGGTA CATCCTGGGG CTGGGGGATC GACATGGAGA AAACATCCTG 
TTCGACTCCA CCAATGGGGA CTGTGTGCAT GTGGACTTCA ACTGTCTCTT CAACAAGGGA 
GAAACATTTG ACTGGCCAGA GTTGGTGCCC TTCCGTCTAA ACACAACATG GTCAACGCAA 
TGTGTGCTGA GGTCCTTTAT CTTCGACCCC TTGGTGGAAT GGTCCCGAGT GAACAAGAGC 
ACCAGGTCTG TGGTTCCTGA GTCAGGAGAG ATCAGCAATG AGAAGGCTAT GACACACGTG 
AGGGACATAG ACCAGCGGCT TCAGGGGGTG TTCAAGAAGA TGCGAGGGCT TCCACTGTCC 
ATCGAAGGCC ATGTTCACCA TCTCATACAG ATTAATGGTA CATTCACCTT AAAGGCCTAC 
AGTAGGAAAC CACTTGAGCT GGACTACAAG GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_104468 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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