BC1G_03424 cDNA ORF clone, Botrytis cinerea B05.10

Gene
Clones
gRNAs

Summary

Gene Symbol	BC1G_03424
Entrez Gene ID	5439193
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Botrytis cinerea B05.10

mRNA and Protein(s)

mRNA	Protein	Name
XM_024695987.1	XP_024551789.1	hypothetical protein
XM_001558525.1	XP_001558575.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A gapless genome sequence of the fungus Botrytis cinerea.
Van Kan JA, Stassen JH, Mosbach A, Van Der Lee TA, Faino L, Farmer AD, Papasotiriou DG, Zhou S, Seidl MF, Cottam E, Edel D, Hahn M, Schwartz DC, Dietrich RA, Widdison S, Scalliet G
Molecular plant pathology18(1)75-89(2017 01)

Genome update of Botrytis cinerea strains B05.10 and T4.
Staats M, van Kan JA
Eukaryotic cell11(11)1413-4(2012 Nov)

Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea.
Amselem J, Cuomo CA, van Kan JA, Viaud M, Benito EP, Couloux A, Coutinho PM, de Vries RP, Dyer PS, Fillinger S, Fournier E, Gout L, Hahn M, Kohn L, Lapalu N, Plummer KM, Pradier JM, Quévillon E, Sharon A, Simon A, ten Have A, Tudzynski B, Tudzynski P, Wincker P, Andrew M, Anthouard V, Beever RE, Beffa R, Benoit I, Bouzid O, Brault B, Chen Z, Choquer M, Collémare J, Cotton P, Danchin EG, Da Silva C, Gautier A, Giraud C, Giraud T, Gonzalez C, Grossetete S, Güldener U, Henrissat B, Howlett BJ, Kodira C, Kretschmer M, Lappartient A, Leroch M, Levis C, Mauceli E, Neuvéglise C, Oeser B, Pearson M, Poulain J, Poussereau N, Quesneville H, Rascle C, Schumacher J, Ségurens B, Sexton A, Silva E, Sirven C, Soanes DM, Talbot NJ, Templeton M, Yandava C, Yarden O, Zeng Q, Rollins JA, Lebrun MH, Dickman M
PLoS genetics7(8)e1002230(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BC1G_03424 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BC1G_03424 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBc07374	XM_001558525.1 Latest version!	Botryotinia fuckeliana B05.10 hypothetical protein (BC1G_03424) partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBc22252	XM_024695987.1 Latest version!	Botrytis cinerea B05.10 hypothetical protein (BCIN_11g03820), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BC1G_03424 ( XM_001558525.1 ) cDNA ORF clone, Botrytis cinerea B05.10 -> XP_001558575.1 Botryotinia fuckeliana B05.10 hypothetical protein (BC1G_03424) partial mRNA.

Price & Availability↑

CloneID	OBc07374
Clone ID Related Accession (Same CDS sequence)	XM_001558525.1
Accession Version	XM_001558525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1203868800000
Organism	Botrytis cinerea B05.10
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001814562). COMPLETENESS: incomplete on both ends.

ATGGAAGACG TCGATAATAT GGAAGTCGAT ATGGATGACC TCTTTGGTGA CGGCACGGGG 
CTATCGTTGC CATCTAGACC ACCGCCATCA AAAGAACTTC ATCAGAGGGT TGATGAACTG 
AGAGGAAGCG GATGTTGCCA AGGCATTGCC TGGTCAAAGT GGGGAAGCAT TGCTTCTATA 
ACTCCAAACG GATCTGGCCT AGAGTTAAGG AATTTAAGGT GTCACCCGGG AGATGGTACA 
TGGGGTTTAA GTGAAGCTAC AGTGATACCA CAATTGACTA GCAACCTCGA TGGTGGGCCC 
TTGAAGCATC TTTCTTGGAG TCCCACTGGC TCCGAGTTGG CGGTCATTGA TTCCGCTGGC 
CGTGTCACAC TTTTGCAGAT ATTCTCTTCA CTAAATAAAC CTATGATGTG TCGTCCTTGT 
GGTCCTGACC CAGCGGATGA TTTACATGCA GTCGCCTCTA GAGTGCAGCA AGGGCCAGCT 
ATTAAGGAAG CATCGGGGTT TCGGTACGAT GCCTCTACAG CGCCTAACCT TGGACCTACG 
CACCCCATTC CTACCAAATC AGCGTTCGTC TATGTTACTT CTAATGGATC TTTACGTCTC 
TTGTGGCTAC AAAGCAATGG TAAATGGCTC GAGTCACACA CAGAGCTGGA GAGTATTATT 
TCATCCGATG ATTTAATCAC CCATGCAGCA ATATGTGCAG AGAGAGGCCC ATCCCTTTTG 
ATTGCGCTTG CTACTTCCTC GAAGCAGTTG CGTATATTTC GAGCTGTTAT ACACTGGGGT 
CTTCCTCAAA CGCAAGAAAA AGTAACCTCA ATGACAATAG CCTTGAATGC AACGCTACAG 
ACCAAGCCTC TTACTGTAAC GAGCTGGTTG CACGATGTTC CAGGTGATAC TTTGAATGCT 
TCTCATCTGG AATCATCAAT GGTACAACTT TCTCACCTAA AGTTTTTATC TCCAACTGTA 
GATAGTAAAA ATCACATGAC TCCACCAATT GTTATGGCTG TTCGTTCTTA TTTGCCTGGA 
TCGACGTCTC ATTATAACCA AGAGGTTTAT TCCACTGTAG ATAGGTGGGA GTTCAGGGAA 
AGACCACAAC AACCAGTACA TTCTGCCTTT GAAAATTTGT CATCGCGTAA GAGTACCGAT 
GGCTCACAGG CGACAAAACC TGTGGTTACT GCTCAGCTCA AGAAACTCGA AAGTTTCACG 
GTAAATAAAA TTATTGTTGC CGTGCAAACT ATATATCTTG GAAGGGTCAT CAACTTTGCA 
TATAGTGATA GTTCGGTCGA TTATCGTGAT CGGATCACCA TGAATGAAAC ATTCAATGAT 
GACGATTTAG ATCGGGTTGT GCATCTTTCT CAAATTGGAT TTTCCTACAC CGAAGATGAA 
CCTTGTTTAC AGGTTGCGTT GTCCCCAAGT ACTTGCTCAC TGGTGCAGAT AAGAAATGAC 
GGAAAGGTCA AATGGAAGCA ATTAGACTAT CATTTGGGAG ATATTGGGTC AACGATGGAA 
CACCCACGTT ATGCCGCAAT GATAGCTGCT CTATCCATAT CATGTTCTAC ATCAGTCATT 
ATGTCTATAA ACTATGACGA CATAATTGCT ACTGCACACA ATCTCGCAGA AACGAAAGAC 
AACCTTGCAT ATGATTGGCT CAATGAACTA GCTAAAATTC TGAAGATTAT TGCTGATTAT 
TCGGAAGAAG CACATTATGA TGTATTAATA CGGAATACCA TGATACAATT GTGTCTTAGC 
ATTCAGAATT CGCTAGGCTT CACAGGCGAA TTCAATGCTC GTAAATTTGC TGGAAAATTC 
TCATGGCTCG TTTTACAGTT GCGGAATACT GTTGTGCTTG TCACTATGGC TGCAAACATG 
AAAGTCCCCG GTCCAACTCA TGATACGAAG TCAACGCCCC TCGATGATCC AGAGGTAATC 
ACTGCCCTTG CCGGTTCTGT TCGCTGGGTT TTAGATCTTA TGGCCTGGAT AACCGACACT 
CTCCTCGAAC TTCCCAGCAC CCTTCCAGAA GATCTATCCG TAACAAATCC CACCAAACTC 
TCACTCCCAG AACTTCTTGC CTACCTTCAT GCCACCAACA ACGTAACCCT CCACCTCCTG 
CTTTCTTCTC CTACCCGTGG TTTCCTTACT GCTATCTGTC GCCGCCTCCA ACATCTTGAC 
TACGTAGCTC GGAAAGCCAT CGCAACAAAC ACCAACCACC CGAACCAAGC AGGACAAGGG 
CAGCAAAGTA TTTCCCCGAC ACTGAGAGCC GCTTATGTAC AAATTGCCAA CTTGACGAGT 
AATTGCATCG TCCGTATCAA AACATTCGAA GCCCTTCTCT CATCCCTAAC CTCCTCAATC 
AAATCCGCAT ACGCAAATCA TAAACCCCCT CTAGCAGGAA ACACAAATAA TAGCACAGGT 
CCTTCGCGCA ATAATCTCGA AATAAAAATG CTTCTAGGCG GCGAAATCCC CACAGCATTT 
AAAGCAGTTA TCATCGATCT CTTCAGAGGA GTACAAGATA ACGAACCTAA AACTCAAAAC 
GATTTGGGGG CCAATGCAGC AGCACCCGGT TACTTATCTA GCGTTCGTCT AGAAATCGAT 
CCTGCAAATC TGTGGTTTGC AGATTTCACG ATGTTGGAAG TGGACGAGGA TGAGGAATCG 
GTGGCGAAGA GAAAGGGCTC AGGGGTTACG ATGGATTGTT TCAGGAAAAC TTGGCTGATC 
AACCCTCCCT CAACTTCCTC TAAATCATCT TTGAACAAAG CGATTGAGAA TGGAAACTCG 
GTGGATAAGG ATAATTCACT ACCAACGATT CAGGGGATGC CTACACCGCA AGGAGCAGGG 
GGCAGTGGTA ATACAAATGG CAATGCCAAC GGCAATGCAA ATGGGGGACA CGGAAGTGGA 
AATACAACAA AGCGCTGGAG AAGATGTGCA AGATGCGCAT CGGTCATGGA AGATGTGTTG 
AGTCAGAGAC CGGCGTTACA GTGGTTGGTT ATGCAGCAGA GAAGATGTTT TTGTGGAGGC 
TATTGGGATA CACTTGTTAA TGGGGAGATG GTCGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001558575.1
CDS	1..3039
Translation	MEDVDNMEVDMDDLFGDGTGLSLPSRPPPSKELHQRVDELRGSGCCQGIAWSKWGSIASITPNGSGLELRNLRCHPGDGTWGLSEATVIPQLTSNLDGGPLKHLSWSPTGSELAVIDSAGRVTLLQIFSSLNKPMMCRPCGPDPADDLHAVASRVQQGPAIKEASGFRYDASTAPNLGPTHPIPTKSAFVYVTSNGSLRLLWLQSNGKWLESHTELESIISSDDLITHAAICAERGPSLLIALATSSKQLRIFRAVIHWGLPQTQEKVTSMTIALNATLQTKPLTVTSWLHDVPGDTLNASHLESSMVQLSHLKFLSPTVDSKNHMTPPIVMAVRSYLPGSTSHYNQEVYSTVDRWEFRERPQQPVHSAFENLSSRKSTDGSQATKPVVTAQLKKLESFTVNKIIVAVQTIYLGRVINFAYSDSSVDYRDRITMNETFNDDDLDRVVHLSQIGFSYTEDEPCLQVALSPSTCSLVQIRNDGKVKWKQLDYHLGDIGSTMEHPRYAAMIAALSISCSTSVIMSINYDDIIATAHNLAETKDNLAYDWLNELAKILKIIADYSEEAHYDVLIRNTMIQLCLSIQNSLGFTGEFNARKFAGKFSWLVLQLRNTVVLVTMAANMKVPGPTHDTKSTPLDDPEVITALAGSVRWVLDLMAWITDTLLELPSTLPEDLSVTNPTKLSLPELLAYLHATNNVTLHLLLSSPTRGFLTAICRRLQHLDYVARKAIATNTNHPNQAGQGQQSISPTLRAAYVQIANLTSNCIVRIKTFEALLSSLTSSIKSAYANHKPPLAGNTNNSTGPSRNNLEIKMLLGGEIPTAFKAVIIDLFRGVQDNEPKTQNDLGANAAAPGYLSSVRLEIDPANLWFADFTMLEVDEDEESVAKRKGSGVTMDCFRKTWLINPPSTSSKSSLNKAIENGNSVDKDNSLPTIQGMPTPQGAGGSGNTNGNANGNANGGHGSGNTTKRWRRCARCASVMEDVLSQRPALQWLVMQQRRCFCGGYWDTLVNGEMVA

Target ORF information:

RefSeq Version	XM_001558525.1
Organism	Botrytis cinerea B05.10
Definition	Botryotinia fuckeliana B05.10 hypothetical protein (BC1G_03424) partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001558525.1

ORF Insert Sequence:

ATGGAAGACG TCGATAATAT GGAAGTCGAT ATGGATGACC TCTTTGGTGA CGGCACGGGG 
CTATCGTTGC CATCTAGACC ACCGCCATCA AAAGAACTTC ATCAGAGGGT TGATGAACTG 
AGAGGAAGCG GATGTTGCCA AGGCATTGCC TGGTCAAAGT GGGGAAGCAT TGCTTCTATA 
ACTCCAAACG GATCTGGCCT AGAGTTAAGG AATTTAAGGT GTCACCCGGG AGATGGTACA 
TGGGGTTTAA GTGAAGCTAC AGTGATACCA CAATTGACTA GCAACCTCGA TGGTGGGCCC 
TTGAAGCATC TTTCTTGGAG TCCCACTGGC TCCGAGTTGG CGGTCATTGA TTCCGCTGGC 
CGTGTCACAC TTTTGCAGAT ATTCTCTTCA CTAAATAAAC CTATGATGTG TCGTCCTTGT 
GGTCCTGACC CAGCGGATGA TTTACATGCA GTCGCCTCTA GAGTGCAGCA AGGGCCAGCT 
ATTAAGGAAG CATCGGGGTT TCGGTACGAT GCCTCTACAG CGCCTAACCT TGGACCTACG 
CACCCCATTC CTACCAAATC AGCGTTCGTC TATGTTACTT CTAATGGATC TTTACGTCTC 
TTGTGGCTAC AAAGCAATGG TAAATGGCTC GAGTCACACA CAGAGCTGGA GAGTATTATT 
TCATCCGATG ATTTAATCAC CCATGCAGCA ATATGTGCAG AGAGAGGCCC ATCCCTTTTG 
ATTGCGCTTG CTACTTCCTC GAAGCAGTTG CGTATATTTC GAGCTGTTAT ACACTGGGGT 
CTTCCTCAAA CGCAAGAAAA AGTAACCTCA ATGACAATAG CCTTGAATGC AACGCTACAG 
ACCAAGCCTC TTACTGTAAC GAGCTGGTTG CACGATGTTC CAGGTGATAC TTTGAATGCT 
TCTCATCTGG AATCATCAAT GGTACAACTT TCTCACCTAA AGTTTTTATC TCCAACTGTA 
GATAGTAAAA ATCACATGAC TCCACCAATT GTTATGGCTG TTCGTTCTTA TTTGCCTGGA 
TCGACGTCTC ATTATAACCA AGAGGTTTAT TCCACTGTAG ATAGGTGGGA GTTCAGGGAA 
AGACCACAAC AACCAGTACA TTCTGCCTTT GAAAATTTGT CATCGCGTAA GAGTACCGAT 
GGCTCACAGG CGACAAAACC TGTGGTTACT GCTCAGCTCA AGAAACTCGA AAGTTTCACG 
GTAAATAAAA TTATTGTTGC CGTGCAAACT ATATATCTTG GAAGGGTCAT CAACTTTGCA 
TATAGTGATA GTTCGGTCGA TTATCGTGAT CGGATCACCA TGAATGAAAC ATTCAATGAT 
GACGATTTAG ATCGGGTTGT GCATCTTTCT CAAATTGGAT TTTCCTACAC CGAAGATGAA 
CCTTGTTTAC AGGTTGCGTT GTCCCCAAGT ACTTGCTCAC TGGTGCAGAT AAGAAATGAC 
GGAAAGGTCA AATGGAAGCA ATTAGACTAT CATTTGGGAG ATATTGGGTC AACGATGGAA 
CACCCACGTT ATGCCGCAAT GATAGCTGCT CTATCCATAT CATGTTCTAC ATCAGTCATT 
ATGTCTATAA ACTATGACGA CATAATTGCT ACTGCACACA ATCTCGCAGA AACGAAAGAC 
AACCTTGCAT ATGATTGGCT CAATGAACTA GCTAAAATTC TGAAGATTAT TGCTGATTAT 
TCGGAAGAAG CACATTATGA TGTATTAATA CGGAATACCA TGATACAATT GTGTCTTAGC 
ATTCAGAATT CGCTAGGCTT CACAGGCGAA TTCAATGCTC GTAAATTTGC TGGAAAATTC 
TCATGGCTCG TTTTACAGTT GCGGAATACT GTTGTGCTTG TCACTATGGC TGCAAACATG 
AAAGTCCCCG GTCCAACTCA TGATACGAAG TCAACGCCCC TCGATGATCC AGAGGTAATC 
ACTGCCCTTG CCGGTTCTGT TCGCTGGGTT TTAGATCTTA TGGCCTGGAT AACCGACACT 
CTCCTCGAAC TTCCCAGCAC CCTTCCAGAA GATCTATCCG TAACAAATCC CACCAAACTC 
TCACTCCCAG AACTTCTTGC CTACCTTCAT GCCACCAACA ACGTAACCCT CCACCTCCTG 
CTTTCTTCTC CTACCCGTGG TTTCCTTACT GCTATCTGTC GCCGCCTCCA ACATCTTGAC 
TACGTAGCTC GGAAAGCCAT CGCAACAAAC ACCAACCACC CGAACCAAGC AGGACAAGGG 
CAGCAAAGTA TTTCCCCGAC ACTGAGAGCC GCTTATGTAC AAATTGCCAA CTTGACGAGT 
AATTGCATCG TCCGTATCAA AACATTCGAA GCCCTTCTCT CATCCCTAAC CTCCTCAATC 
AAATCCGCAT ACGCAAATCA TAAACCCCCT CTAGCAGGAA ACACAAATAA TAGCACAGGT 
CCTTCGCGCA ATAATCTCGA AATAAAAATG CTTCTAGGCG GCGAAATCCC CACAGCATTT 
AAAGCAGTTA TCATCGATCT CTTCAGAGGA GTACAAGATA ACGAACCTAA AACTCAAAAC 
GATTTGGGGG CCAATGCAGC AGCACCCGGT TACTTATCTA GCGTTCGTCT AGAAATCGAT 
CCTGCAAATC TGTGGTTTGC AGATTTCACG ATGTTGGAAG TGGACGAGGA TGAGGAATCG 
GTGGCGAAGA GAAAGGGCTC AGGGGTTACG ATGGATTGTT TCAGGAAAAC TTGGCTGATC 
AACCCTCCCT CAACTTCCTC TAAATCATCT TTGAACAAAG CGATTGAGAA TGGAAACTCG 
GTGGATAAGG ATAATTCACT ACCAACGATT CAGGGGATGC CTACACCGCA AGGAGCAGGG 
GGCAGTGGTA ATACAAATGG CAATGCCAAC GGCAATGCAA ATGGGGGACA CGGAAGTGGA 
AATACAACAA AGCGCTGGAG AAGATGTGCA AGATGCGCAT CGGTCATGGA AGATGTGTTG 
AGTCAGAGAC CGGCGTTACA GTGGTTGGTT ATGCAGCAGA GAAGATGTTT TTGTGGAGGC 
TATTGGGATA CACTTGTTAA TGGGGAGATG GTCGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BC1G_03424 ( XM_024695987.1 ) cDNA ORF clone, Botrytis cinerea B05.10 -> XP_024551789.1 Botrytis cinerea B05.10 hypothetical protein (BCIN_11g03820), mRNA.

Price & Availability↑

CloneID	OBc22252
Clone ID Related Accession (Same CDS sequence)	XM_024695987.1
Accession Version	XM_024695987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523808000000
Organism	Botrytis cinerea B05.10
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_037320).

ATGCCACTCA TGATGGAAGA CGTCGATAAT ATGGAAGTCG ATATGGATGA CCTCTTTGGT 
GACGGCACGG GGCTATCGTT GCCATCTAGA CCACCGCCAT CAAAAGAACT TCATCAGAGG 
GTTGATGAAC TGAGAGGAAG CGGATGTTGC CAAGGCATTG CCTGGTCAAA GTGGGGAAGC 
ATTGCTTCTA TAACTCCAAA CGGATCTGGC CTAGAGTTAA GGAATTTAAG GTGTCACCCG 
GGAGATGGTA CATGGGGTTT AAGTGAAGCT ACAGTGATAC CACAATTGAC TAGCAACCTC 
GATGGTGGGC CCTTGAAGCA TCTTTCTTGG AGTCCCACTG GCTCCGAGTT GGCGGTCATT 
GATTCCGCTG GCCGTGTCAC ACTTTTGCAG ATATTCTCTT CACTAAATAA ACCTATGATG 
TGTCGTCCTT GTGGTCCTGA CCCAGCGGAT GATTTACATG CAGTCGTGGG TTGTTTCTGG 
CTGAATCTAG GTCCATTTCA GAATAGAACT GCCTCTAGAG TGCAGCAAGG GCCAGCTATT 
AAGGAAGCAT CGGGGTTTCG GTACGATGCC TCTACAGCGC CTAACCTTGG ACCTACGCAC 
CCCATTCCTA CCAAATCAGC GTTCGTCTAT GTTACTTCTA ATGGATCTTT ACGTCTCTTG 
TGGCTACAAA GCAATGGTAA ATGGCTCGAG TCACACACAG AGCTGGAGAG TATTATTTCA 
TCCGATGATT TAATCACCCA TGCAGCAATA TGTGCAGAGA GAGGCCCATC CCTTTTGATT 
GCGCTTGCTA CTTCCTCGAA GCAGTTGCGT ATATTTCGAG CTGTTATACA CTGGGGTCTT 
CCTCAAACGC AAGAAAAAGT AACCTCAATG ACAATAGCCT TGAATGCAAC GCTACAGACC 
AAGCCTCTTA CTGTAACGAG CTGGTTGCAC GATGTTCCAG GTGATACTTT GAATGCTTCT 
CATCTGGAAT CATCAATGGT ACAACTTTCT CACCTAAAGT TTTTATCTCC AACTGTAGAT 
AGTAAAAATC ACATGACTCC ACCAATTGTT ATGGCTGTTC GTTCTTATTT GCCTGGATCG 
ACGTCTCATT ATAACCAAGA GGTTTATTCC ACTGTAGATA GGTGGGAGTT CAGGGAAAGA 
CCACAACAAC CAGTACATTC TGCCTTTGAA AATTTGTCAT CGCGTAAGAG TACCGATGGC 
TCACAGGCGA CAAAACCTGT GGTTACTGCT CAGCTCAAGA AACTCGAAAG TTTCACGGTA 
AATAAAATTA TTGTTGCCGT GCAAACTATA TATCTTGGAA GGGTCATCAA CTTTGCATAT 
AGTGATAGTT CGGTCGATTA TCGTGATCGG ATCACCATGA ATGAAACATT CAATGATGAC 
GATTTAGATC GGGTTGTGCA TCTTTCTCAA ATTGGATTTT CCTACACCGA AGATGAACCT 
TGTTTACAGG TTGCGTTGTC CCCAAGTACT TGCTCACTGG TGCAGATAAG AAATGACGGA 
AAGGTCAAAT GGAAGCAATT AGACTATCAT TTGGGAGATA TTGGGTCAAC GATGGAACAC 
CCACGTTATG CCGCAATGAT AGCTGCTCTA TCCATATCAT GTTCTACATC AGTCATTATG 
TCTATAAACT ATGACGACAT AATTGCTACT GCACACAATC TCGCAGAAAC GAAAGACAAC 
CTTGCATATG ATTGGCTCAA TGAACTAGCT AAAATTCTGA AGATTATTGC TGATTATTCG 
GAAGAAGCAC ATTATGATGT ATTAATACGG AATACCATGA TACAATTGTG TCTTAGCATT 
CAGAATTCGC TAGGCTTCAC AGGCGAATTC AATGCTCGTA AATTTGCTGG AAAATTCTCA 
TGGCTCGTTT TACAGTTGCG GAATACTGTT GTGCTTGTCA CTATGGCTGC AAACATGAAA 
GTCCCCGGTC CAACTCATGA TACGAAGTCA ACGCCCCTCG ATGATCCAGA GGTAATCACT 
GCCCTTGCCG GTTCTGTTCG CTGGGTTTTA GATCTTATGG CCTGGATAAC CGACACTCTC 
CTCGAACTTC CCAGCACCCT TCCAGAAGAT CTATCCGTAA CAAATCCCAC CAAACTCTCA 
CTCCCAGAAC TTCTTGCCTA CCTTCATGCC ACCAACAACG TAACCCTCCA CCTCCTGCTT 
TCTTCTCCTA CCCGTGGTTT CCTTACTGCT ATCTGTCGCC GCCTCCAACA TCTTGACTAC 
GTAGCTCGGA AAGCCATCGC AACAAACACC AACCACCCGA ACCAAGCAGG ACAAGGGCAG 
CAAAGTATTT CCCCGACACT GAGAGCCGCT TATGTACAAA TTGCCAACTT GACGAGTAAT 
TGCATCGTCC GTATCAAAAC ATTCGAAGCC CTTCTCTCAT CCCTAACCTC CTCAATCAAA 
TCCGCATACG CAAATCATAA ACCCCCTCTA GCAGGAAACA CAAATAATAG CACAGGTCCT 
TCGCGCAATA ATCTCGAAAT AAAAATGCTT CTAGGCGGCG AAATCCCCAC AGCATTTAAA 
GCAGTTATCA TCGATCTCTT CAGAGGAGTA CAAGATAACG AACCTAAAAC TCAAAACGAT 
TTGGGGGCCA ATGCAGCAGC ACCCGGTTAC TTATCTAGCG TTCGTCTAGA AATCGATCCT 
GCAAATCTGT GGTTTGCAGA TTTCACGATG TTGGAAGTGG ACGAGGATGA GGAATCGGTG 
GCGAAGAGAA AGGGCTCAGG GGTTACGATG GATTGTTTCA GGAAAACTTG GCTGATCAAC 
CCTCCCTCAA CTTCCTCTAA ATCATCTTTG AACAAAGCGA TTGAGAATGG AAACTCGGTG 
GATAAGGATA ATTCACTACC AACGATTCAG GGGATGCCTA CACCGCAAGG AGCAGGGGGC 
AGTGGTAATA CAAATGGCAA TGCCAACGGC AATGCAAATG GGGGACACGG AAGTGGAAAT 
ACAACAAAGC GCTGGAGAAG ATGTGCAAGA TGCGCATCGG TCATGGAAGA TGTGTTGAGT 
CAGAGACCGG CGTTACAGTG GTTGGTTATG CAGCAGAGAA GATGTTTTTG TGGAGGCTAT 
TGGGATACAC TTGTTAATGG GGAGATGGTC GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024551789.1
CDS	490..3585
Translation	MPLMMEDVDNMEVDMDDLFGDGTGLSLPSRPPPSKELHQRVDELRGSGCCQGIAWSKWGSIASITPNGSGLELRNLRCHPGDGTWGLSEATVIPQLTSNLDGGPLKHLSWSPTGSELAVIDSAGRVTLLQIFSSLNKPMMCRPCGPDPADDLHAVVGCFWLNLGPFQNRTASRVQQGPAIKEASGFRYDASTAPNLGPTHPIPTKSAFVYVTSNGSLRLLWLQSNGKWLESHTELESIISSDDLITHAAICAERGPSLLIALATSSKQLRIFRAVIHWGLPQTQEKVTSMTIALNATLQTKPLTVTSWLHDVPGDTLNASHLESSMVQLSHLKFLSPTVDSKNHMTPPIVMAVRSYLPGSTSHYNQEVYSTVDRWEFRERPQQPVHSAFENLSSRKSTDGSQATKPVVTAQLKKLESFTVNKIIVAVQTIYLGRVINFAYSDSSVDYRDRITMNETFNDDDLDRVVHLSQIGFSYTEDEPCLQVALSPSTCSLVQIRNDGKVKWKQLDYHLGDIGSTMEHPRYAAMIAALSISCSTSVIMSINYDDIIATAHNLAETKDNLAYDWLNELAKILKIIADYSEEAHYDVLIRNTMIQLCLSIQNSLGFTGEFNARKFAGKFSWLVLQLRNTVVLVTMAANMKVPGPTHDTKSTPLDDPEVITALAGSVRWVLDLMAWITDTLLELPSTLPEDLSVTNPTKLSLPELLAYLHATNNVTLHLLLSSPTRGFLTAICRRLQHLDYVARKAIATNTNHPNQAGQGQQSISPTLRAAYVQIANLTSNCIVRIKTFEALLSSLTSSIKSAYANHKPPLAGNTNNSTGPSRNNLEIKMLLGGEIPTAFKAVIIDLFRGVQDNEPKTQNDLGANAAAPGYLSSVRLEIDPANLWFADFTMLEVDEDEESVAKRKGSGVTMDCFRKTWLINPPSTSSKSSLNKAIENGNSVDKDNSLPTIQGMPTPQGAGGSGNTNGNANGNANGGHGSGNTTKRWRRCARCASVMEDVLSQRPALQWLVMQQRRCFCGGYWDTLVNGEMVA

Target ORF information:

RefSeq Version	XM_024695987.1
Organism	Botrytis cinerea B05.10
Definition	Botrytis cinerea B05.10 hypothetical protein (BCIN_11g03820), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024695987.1

ORF Insert Sequence:

ATGCCACTCA TGATGGAAGA CGTCGATAAT ATGGAAGTCG ATATGGATGA CCTCTTTGGT 
GACGGCACGG GGCTATCGTT GCCATCTAGA CCACCGCCAT CAAAAGAACT TCATCAGAGG 
GTTGATGAAC TGAGAGGAAG CGGATGTTGC CAAGGCATTG CCTGGTCAAA GTGGGGAAGC 
ATTGCTTCTA TAACTCCAAA CGGATCTGGC CTAGAGTTAA GGAATTTAAG GTGTCACCCG 
GGAGATGGTA CATGGGGTTT AAGTGAAGCT ACAGTGATAC CACAATTGAC TAGCAACCTC 
GATGGTGGGC CCTTGAAGCA TCTTTCTTGG AGTCCCACTG GCTCCGAGTT GGCGGTCATT 
GATTCCGCTG GCCGTGTCAC ACTTTTGCAG ATATTCTCTT CACTAAATAA ACCTATGATG 
TGTCGTCCTT GTGGTCCTGA CCCAGCGGAT GATTTACATG CAGTCGTGGG TTGTTTCTGG 
CTGAATCTAG GTCCATTTCA GAATAGAACT GCCTCTAGAG TGCAGCAAGG GCCAGCTATT 
AAGGAAGCAT CGGGGTTTCG GTACGATGCC TCTACAGCGC CTAACCTTGG ACCTACGCAC 
CCCATTCCTA CCAAATCAGC GTTCGTCTAT GTTACTTCTA ATGGATCTTT ACGTCTCTTG 
TGGCTACAAA GCAATGGTAA ATGGCTCGAG TCACACACAG AGCTGGAGAG TATTATTTCA 
TCCGATGATT TAATCACCCA TGCAGCAATA TGTGCAGAGA GAGGCCCATC CCTTTTGATT 
GCGCTTGCTA CTTCCTCGAA GCAGTTGCGT ATATTTCGAG CTGTTATACA CTGGGGTCTT 
CCTCAAACGC AAGAAAAAGT AACCTCAATG ACAATAGCCT TGAATGCAAC GCTACAGACC 
AAGCCTCTTA CTGTAACGAG CTGGTTGCAC GATGTTCCAG GTGATACTTT GAATGCTTCT 
CATCTGGAAT CATCAATGGT ACAACTTTCT CACCTAAAGT TTTTATCTCC AACTGTAGAT 
AGTAAAAATC ACATGACTCC ACCAATTGTT ATGGCTGTTC GTTCTTATTT GCCTGGATCG 
ACGTCTCATT ATAACCAAGA GGTTTATTCC ACTGTAGATA GGTGGGAGTT CAGGGAAAGA 
CCACAACAAC CAGTACATTC TGCCTTTGAA AATTTGTCAT CGCGTAAGAG TACCGATGGC 
TCACAGGCGA CAAAACCTGT GGTTACTGCT CAGCTCAAGA AACTCGAAAG TTTCACGGTA 
AATAAAATTA TTGTTGCCGT GCAAACTATA TATCTTGGAA GGGTCATCAA CTTTGCATAT 
AGTGATAGTT CGGTCGATTA TCGTGATCGG ATCACCATGA ATGAAACATT CAATGATGAC 
GATTTAGATC GGGTTGTGCA TCTTTCTCAA ATTGGATTTT CCTACACCGA AGATGAACCT 
TGTTTACAGG TTGCGTTGTC CCCAAGTACT TGCTCACTGG TGCAGATAAG AAATGACGGA 
AAGGTCAAAT GGAAGCAATT AGACTATCAT TTGGGAGATA TTGGGTCAAC GATGGAACAC 
CCACGTTATG CCGCAATGAT AGCTGCTCTA TCCATATCAT GTTCTACATC AGTCATTATG 
TCTATAAACT ATGACGACAT AATTGCTACT GCACACAATC TCGCAGAAAC GAAAGACAAC 
CTTGCATATG ATTGGCTCAA TGAACTAGCT AAAATTCTGA AGATTATTGC TGATTATTCG 
GAAGAAGCAC ATTATGATGT ATTAATACGG AATACCATGA TACAATTGTG TCTTAGCATT 
CAGAATTCGC TAGGCTTCAC AGGCGAATTC AATGCTCGTA AATTTGCTGG AAAATTCTCA 
TGGCTCGTTT TACAGTTGCG GAATACTGTT GTGCTTGTCA CTATGGCTGC AAACATGAAA 
GTCCCCGGTC CAACTCATGA TACGAAGTCA ACGCCCCTCG ATGATCCAGA GGTAATCACT 
GCCCTTGCCG GTTCTGTTCG CTGGGTTTTA GATCTTATGG CCTGGATAAC CGACACTCTC 
CTCGAACTTC CCAGCACCCT TCCAGAAGAT CTATCCGTAA CAAATCCCAC CAAACTCTCA 
CTCCCAGAAC TTCTTGCCTA CCTTCATGCC ACCAACAACG TAACCCTCCA CCTCCTGCTT 
TCTTCTCCTA CCCGTGGTTT CCTTACTGCT ATCTGTCGCC GCCTCCAACA TCTTGACTAC 
GTAGCTCGGA AAGCCATCGC AACAAACACC AACCACCCGA ACCAAGCAGG ACAAGGGCAG 
CAAAGTATTT CCCCGACACT GAGAGCCGCT TATGTACAAA TTGCCAACTT GACGAGTAAT 
TGCATCGTCC GTATCAAAAC ATTCGAAGCC CTTCTCTCAT CCCTAACCTC CTCAATCAAA 
TCCGCATACG CAAATCATAA ACCCCCTCTA GCAGGAAACA CAAATAATAG CACAGGTCCT 
TCGCGCAATA ATCTCGAAAT AAAAATGCTT CTAGGCGGCG AAATCCCCAC AGCATTTAAA 
GCAGTTATCA TCGATCTCTT CAGAGGAGTA CAAGATAACG AACCTAAAAC TCAAAACGAT 
TTGGGGGCCA ATGCAGCAGC ACCCGGTTAC TTATCTAGCG TTCGTCTAGA AATCGATCCT 
GCAAATCTGT GGTTTGCAGA TTTCACGATG TTGGAAGTGG ACGAGGATGA GGAATCGGTG 
GCGAAGAGAA AGGGCTCAGG GGTTACGATG GATTGTTTCA GGAAAACTTG GCTGATCAAC 
CCTCCCTCAA CTTCCTCTAA ATCATCTTTG AACAAAGCGA TTGAGAATGG AAACTCGGTG 
GATAAGGATA ATTCACTACC AACGATTCAG GGGATGCCTA CACCGCAAGG AGCAGGGGGC 
AGTGGTAATA CAAATGGCAA TGCCAACGGC AATGCAAATG GGGGACACGG AAGTGGAAAT 
ACAACAAAGC GCTGGAGAAG ATGTGCAAGA TGCGCATCGG TCATGGAAGA TGTGTTGAGT 
CAGAGACCGG CGTTACAGTG GTTGGTTATG CAGCAGAGAA GATGTTTTTG TGGAGGCTAT 
TGGGATACAC TTGTTAATGG GGAGATGGTC GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A gapless genome sequence of the fungus Botrytis cinerea.
Molecular plant pathology18(1)75-89(2017 01)
Van Kan JA,Stassen JH,Mosbach A,Van Der Lee TA,Faino L,Farmer AD,Papasotiriou DG,Zhou S,Seidl MF,Cottam E,Edel D,Hahn M,Schwartz DC,Dietrich RA,Widdison S,Scalliet G

Genome update of Botrytis cinerea strains B05.10 and T4.
Eukaryotic cell11(11)1413-4(2012 Nov)
Staats M,van Kan JA

Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea.
PLoS genetics7(8)e1002230(2011 Aug)
Amselem J,Cuomo CA,van Kan JA,Viaud M,Benito EP,Couloux A,Coutinho PM,de Vries RP,Dyer PS,Fillinger S,Fournier E,Gout L,Hahn M,Kohn L,Lapalu N,Plummer KM,Pradier JM,Quévillon E,Sharon A,Simon A,ten Have A,Tudzynski B,Tudzynski P,Wincker P,Andrew M,Anthouard V,Beever RE,Beffa R,Benoit I,Bouzid O,Brault B,Chen Z,Choquer M,Collémare J,Cotton P,Danchin EG,Da Silva C,Gautier A,Giraud C,Giraud T,Gonzalez C,Grossetete S,Güldener U,Henrissat B,Howlett BJ,Kodira C,Kretschmer M,Lappartient A,Leroch M,Levis C,Mauceli E,Neuvéglise C,Oeser B,Pearson M,Poulain J,Poussereau N,Quesneville H,Rascle C,Schumacher J,Ségurens B,Sexton A,Silva E,Sirven C,Soanes DM,Talbot NJ,Templeton M,Yandava C,Yarden O,Zeng Q,Rollins JA,Lebrun MH,Dickman M

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BC1G_03424 cDNA ORF clone, Botrytis cinerea B05.10

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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