BC1G_07127 cDNA ORF clone, Botrytis cinerea B05.10

Gene
Clones
gRNAs

Summary

Gene Symbol	BC1G_07127
Entrez Gene ID	5435093
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Botrytis cinerea B05.10

mRNA and Protein(s)

mRNA	Protein	Name
XM_024694997.1	XP_024550790.1	hypothetical protein
XM_001554489.1	XP_001554539.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A gapless genome sequence of the fungus Botrytis cinerea.
Van Kan JA, Stassen JH, Mosbach A, Van Der Lee TA, Faino L, Farmer AD, Papasotiriou DG, Zhou S, Seidl MF, Cottam E, Edel D, Hahn M, Schwartz DC, Dietrich RA, Widdison S, Scalliet G
Molecular plant pathology18(1)75-89(2017 01)

Genome update of Botrytis cinerea strains B05.10 and T4.
Staats M, van Kan JA
Eukaryotic cell11(11)1413-4(2012 Nov)

Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea.
Amselem J, Cuomo CA, van Kan JA, Viaud M, Benito EP, Couloux A, Coutinho PM, de Vries RP, Dyer PS, Fillinger S, Fournier E, Gout L, Hahn M, Kohn L, Lapalu N, Plummer KM, Pradier JM, Quévillon E, Sharon A, Simon A, ten Have A, Tudzynski B, Tudzynski P, Wincker P, Andrew M, Anthouard V, Beever RE, Beffa R, Benoit I, Bouzid O, Brault B, Chen Z, Choquer M, Collémare J, Cotton P, Danchin EG, Da Silva C, Gautier A, Giraud C, Giraud T, Gonzalez C, Grossetete S, Güldener U, Henrissat B, Howlett BJ, Kodira C, Kretschmer M, Lappartient A, Leroch M, Levis C, Mauceli E, Neuvéglise C, Oeser B, Pearson M, Poulain J, Poussereau N, Quesneville H, Rascle C, Schumacher J, Ségurens B, Sexton A, Silva E, Sirven C, Soanes DM, Talbot NJ, Templeton M, Yandava C, Yarden O, Zeng Q, Rollins JA, Lebrun MH, Dickman M
PLoS genetics7(8)e1002230(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BC1G_07127 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BC1G_07127 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBc05208	XM_001554489.1 Latest version!	Botryotinia fuckeliana B05.10 hypothetical protein (BC1G_07127) partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBc21195	XM_024694997.1 Latest version!	Botrytis cinerea B05.10 hypothetical protein (BCIN_09g02510), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BC1G_07127 ( XM_001554489.1 ) cDNA ORF clone, Botrytis cinerea B05.10 -> XP_001554539.1 Botryotinia fuckeliana B05.10 hypothetical protein (BC1G_07127) partial mRNA.

Price & Availability↑

CloneID	OBc05208
Clone ID Related Accession (Same CDS sequence)	XM_001554489.1
Accession Version	XM_001554489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1203868800000
Organism	Botrytis cinerea B05.10
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001814536). COMPLETENESS: incomplete on both ends.

ATGGCGAAGA AACGCCAGAA GACTTCGCGT CCAGCACCAT GTGTTGAAGA TGGATCCGAT 
TTAGAGGATG AAGATGGCAA TGGTGGTATT GATTCAGACG ATCAAGCACC AGGTCTTCAT 
GATCAAAAGA GGAGAACCGA TGAGAGTAAA CAGTGGAACA CCCTCAGCTT CGCGGAGGGA 
ATCGACTCAA GTCTTCCTCC ACTTACTACT GCCACGGAAT TTATGGAGAT CTTGCTTGAA 
AGATTGTTGA ATGGGGGATT CAAAGACGTT ATAAAGCACC TCAATGGTCG GCCTTTGCGC 
GTCGCCACCG CATGTTCCGG CACTGAAGCA CCCATCCTGT TTCTGAATTC TTTTTCCGAA 
GGATTAAGGA AAAGAGGTCA TGACTTCAGA ATTGAGCATG TCTTCTCATG TGAAATTGAT 
CCGGCTAAAC AATCTTACAT TCGTCGAAAC TTTCATAATG TTCCTATTCT ACGTGATATC 
ACAGAAGTCT TCGAGTGGGA GGGTAACCCT GAAAAGATAG GTTTTATGAC TACCGCTTTT 
GGTAGAACTT ATGCGCTTCC AGCACCGGGT ACAGTTGACG TTATCATCGT TGGTACTTCT 
TGCACCAGCT ATTCCAATTT GAACAACCAA AAGAGTACGG ACTTGGAGAG AGACCAAACT 
GAGTCATCGC GCACTTTCCG CGCATATGTT TTGCTTGTTA AGTATCTAAA AGCAAAGATC 
GCTATCAGCG AAAATGTCCT GAGTGGCCCT TTCGAGGCTA TGATTAAAAT GCTGGGCGAT 
CACGGTTACA TCGGTGGCTC AGCAAACCTC GACAGCAAGC ATTATGGCAT TCCCCAAACC 
CGCCAACGCG GTTATCTCAT TGCCCTCCCT GAATCCTCTC TGGGACCCGA TGAGATAGAA 
GAGTATAAAC TTATAAAGAC ATGGAAAATA TACTATGCTA ACATGAAAGT CATGTGTTCG 
CGCCCTGCTA GCAGTGACGC TGAGCAGTGG ATGGAGCCAT CTGATTCAAA GGCACTCCGG 
TCTCAGGTCG AATTTCAAGT TGAATCACGT GCGGTCGCAA AGTGGGATAC GTGCTGTGCG 
AGGCATTTTG ATTATCGCCA TGCACAGGGC TTCGGTACTT CTAAGCCTAT TACCGATTGG 
CGCAGTGCGG GCAACTATTG TCATCCTGAT AATTGGCCCC GTAATCTTAA AGGCTTTGTA 
GAACGTGTTC ACGACGTTAC TGATGTCAGC CATTTGCGTG GCATATGCCG AGGTTATGAT 
GACCGTTACC TCAGTCGAAA CATCGATTTG TCACAAAACG TTTATGTTCA GCACGATAAC 
GTGAAGTGCG GTCTATTGCC TTGCTTGACA CCAAACGGTG TCATGTTTAA TACTGCACGT 
GGTAGCAAGC TTACTGGTGA AGAATATCTT CGCCTCCAGG GAATTGATAC TAATAGTCTT 
GAACTTGGTG GTATTTCTCA ACAAGACTTG AGAAACCTTG CTGGCAATGC GATGACAGTC 
ACAGTGGTTG GAAGTGTAAC GACGTTCGCG CTTCTTAAGT ACTTCTCAAA GCTCACTCCA 
GGCGATGGCG TCAAAATTCC AGCCCAGGAC ATAGTGAAAC AGAGTTTCCG TGGCGGAGAA 
GACATGCTCA ACTCATATGA AGATATTACG GTTGTTCGGC GGCCTTTGAC TTTTGGAGAA 
TGTGTTCATC TCGTCGAGTC TTCTGCAAAA TACTGCTATT GCGAAGCACA AACTGATATA 
TCACGATCGT CAATTGTCAA ATGCAAGAAC TGTGGGCATG TTTCATGTAC TAACTGTGGG 
ATTAAGCCGC GTCATGCCTA TGAGCCTTTG GATCTCTACA TTGGCCCTCG AGGTACCAAG 
GGTATTACTC CTCGTATTTC GCCTTCGTCC ATCGAGTTCA ATATTGCTGA ACGTCTGCCA 
ATGATGGTTT CAATATCAGG GTTGTTCGCC TCGGACTGCC AAGTGTTATC GATTCTCGAA 
GAAGTTGCTA CTGGTGACAT TGATCATAAT CAGTGGGCTG CCTTGCGTAA TTCTTTTTTA 
AATTGCCTAC AATCCATAGT CAAGTTTCAG AAGCCGGTCC GATCTTTTGC TTGGAATTTT 
ACATGGCTTT CGGAGCACGC CAGATTGGAG TTATCTCTTA GCAACGACAA GATTCAATGG 
GTTCTGTACG CTTTGCCACC TCCTACGGCG GCTGTTAACA GTTCGTTGAG GAGATATCTT 
ACAAAATTTC CTCTCGCAAG AACAGTCCCT ATTAACGCGT CTGATGACAG CTCAAGAACC 
ATATTCGATT GCACATGGCA GATATGGCAG CCAAAGATTC GCACCGCTCA GGCGACTATC 
AGCTATGGAG GCGACCTCAT TTCGACTTAC GAAAGCTCAA TCGATCTTCC TGCCTGGAAA 
GGAGATTTTA TATTCTCAAA GAAAACCATC CAGGTCCTAG ATGATCATCC CGCGTTCGGG 
GAAATCAATG GTGTTTATAT AGCGCATCCG GAATGTCAAC AGGCTTTTAA TAGCTACCAT 
TCCAAGAATG GCACTGCCGG AGGAGATAAT CAAATACATT TTTTCTTCGA TCATGAAGGA 
GGCACAGGGG ACTGGAACAA GCATGGGTTT GTCTTCACTT ACGATCAAAC ACGCAAAGAA 
TTTGGAGTAT GCAGACAAAT CATTGCTTCG CTTTGCACCT CGTGGGAACA GCCCATAGCA 
AAAAAACTTA ATGGTGCTAC TTATCAATAT GGAGATACCG TGTACAAAAG TCTTCCCTTG 
CTAAAAGAAG GAATGAGTGA AGTTGTCGAG ATTACCTCCT TTGGGAGCTG GACCACAACA 
CCCGAAATCA TCAGTCCTAT GGCTACCAAG TTGTCTGACC GATCTTCTAT GGTTGTCTAC 
GAAAAGTTCA ACTCACTTAA TCCAAAGCTT CTTCTCGAGA CTGATTGCAA AAACATGCTT 
AGTGCATTTT CTTGCAAAGC ATATACGAAT GTTATACCTC CTTATTGGGA ACCAGATCAT 
TGGATCAACG TCACTAAGCA AAATGGTACC CAATTTTTTA AGGGCTGTTA CCATCTGTTA 
GCTCGTGGCT TGGTTACTCC AGGAATCGAT GAGGCCGATC CGCCTTGGAT TCCATGCAAC 
AAACAAGGCG ACTCTTCATG TCGGTGCGAC GATTGTGCAC CACCCAAGCC AGATATGCTA 
TGGCATTTGA AGAGAAGCGG GCAAAGAATT CAGCAAACCC CATTCGAAAA TCCACCAGCA 
GCTGCGATCT TTGAGACTTT AACAAAGAAT GCGCCGCCGT CAATGCAGCA ATGGGTCAGA 
TTCCTCAGGA AAGATGCCTT CTTTCAATTC GAGTATCAAT TCCTTATCTG TCCCGCGGTT 
TTGTGTCATC AAGCAGCTGC GCTTCTTAAT TTGTTTCCAC GCCCAGAGAT TCCTGTGCAC 
CTCTCGTGGC GTTTCCTTCC TGGAGCTTGG GGAAGCAGTA AATTACCAAC GCGAATGTTT 
TCATATTTCA ATACAGAAAC CTTAAAGATG TCAGAACAGC CAATTACATT TGTTCCAGGA 
AAGAAGCTCC GCAATGAGCA ACTCAAAACT CTGACATGGA TGGTAAAGCA AGAGAGGTGC 
CCACCGTCTT TCATGGAGAG AGAAATTGTT GAAATTTGTC AAAATGACTT GGCATTCAGA 
CTTGAAGGCC GAGCTTCTCG AATAGTAAAA CACCGTGCTG GACTAATTGC TGCTGCCGTT 
GGATATGGCA AGACGGTTCT TACTTTTGCT TTAATGGGCA GGCAGCGTCG TATGGATCGC 
CTATGGGCCA ACAACCCTAA TATCAATGGT CAAATTCATG TCAAAGCAAC GCTGGTTTTG 
GTTCCAACGC ATCTGACTAT ACAGTGGCGA GATGAGGGAT GCTCGTTCAT TGGTTATGAT 
GCTAAGGATG ATGCAAGAAT TATAACACTC AGATCATTTA CAGACGTTAG GGCTCTGACT 
GTTGAAGATG TTCGGAGAGC AGAGCTAGTA ATTTGCAGCT CTGCCTTGTT CGATAATGAA 
GGATACTTGA AACTCATGGC GAAGAACGCT GGAGTTGTTA ATTTGGCCAA AGGCGCGACT 
GACAGAGCCA AGGAAGCTTG GTATCTTAAT GCTCAGAAAG AGAGACGGGA GCTCTTGGCG 
TATCTTTTGG AAAGGCTCCC AATGGAAGTA GATGAGAACG ACAAAGCAGC TCCTGCCCGA 
AACGCTAAAA TTATCAAGAA CTTCAACAAG AAGACTTTTC AGAAATACAA GAAAAATGTT 
GCGGATGCTG GGGTTAATGA GCCACATATT CCTTCAATGC GGCGTAAGGG CGCAGAACTT 
GTTAAATATC TCATGGAGGA AGCCAAGAAG GATTTCAAGG GCAAGGGCAA ACCCAGAACT 
AATGTCTTGA TTGATGAGGC AGATGCTGAG AAGACGTTCG AGTTCCACTA CTTTGAAGAT 
GATTGTGTTG ATGGCCTTAC TGGGGAGACA CTCCGCAATG ATAAGAATTT GACTAGCGCG 
GAAAGGTTTT TATCCTATGG ACCTGACGCA TCGGCAACCT GGCATTCTCA AAGACTCCAA 
GTTGCTCAAG ATTTTTGCGA TACTTTCATC CGCTACGACG CGAAAAAGGG AAATGGACAA 
TACAATCGCC TTCACCGTTT CACTGTAATG CCGTTTGCCA CAGCACAGTT GGCATGTTAC 
CTCATCATGA GAGCCGACGT TATTAACAAG AGCTACATGA TGCCAAGAGC TAAAGCTAAC 
ACCATTCGAA GTTCGGTCAA ATTGGAAATC TCAAAAGTTT ATGGTAAGGA TACAGACGGT 
CGACTGAGAT TAACCAAAGC CGCATCATTC TTCCCGGAGC ACGCTAGAAA GGATACAAAA 
TATAAACTTA AGACTATCAA AGACTTGAAA GCAGCCGCGC TCAATGAAAT CAATGCTTCG 
AAAAACTATT TGCGCCAACA GATGGAGCTA CTCGATTATT TGGTGACAGA GAAATTAGTC 
TTCAAGGATG CCCAACTGGT TCTTAGATAT AGGAACTGGG AAACTCAAGT TTTAGCCGGA 
TATCGTTACC CCGACCCCGA CGCTCTTCTT GATATCCTCC AAGTTGTCAA AGGCGTTCGA 
AGGACAAAGT CTAGTAAGAC CAAAGATGAC TTTTACTATG AAAATCCTGG TCCTGCTTAT 
GGTCGCCGTC TCTGGCCTTC TCAAACTGAG ACAATCATGC ACAACGGTCG CAAAACGCTT 
GGTCTAATTG CCGAATTTAA GATAGGGCTC ATCGAATTAA ACAAAACTGC TATGGACCTT 
ATGCAATTTA GAGCGGATTA TCGCTATTAT AGGTCTGTGG GGCAGATTGA CCAAGCTGGT 
AGCGTAACAC CTGCAGGAAG TCTCACTTGT AACTGTTGCA ACAAGAGTAC ACCTTTGTAC 
GAAGCAGTCA TTCAAGGTAG CTGTGGCCAT ATCCTATGTA GGAAATGCAA CGAAACCTCA 
TCGGAAGGCA ATTGCCCAAT TGAACACTGT GCTGCGCAAT CACTAGATGA CCAAAAGTAC 
TTGGCGTCAT CCTTATATTC TTCGGCTGGA AAATTTCCCG AGCGCAAAGA ACCAGGAACT 
AAACCCGACG AGCTTTCACT ATTCTGGAAT AACCCAAGTG TTCGAGTCGA CAAGCTGACA 
GCTATGCGGT CGATCTTCTG GAAGCACGAT GACGGCAAGA ATAAATTCAT TGTATTCAGT 
CCCCTACCAG AGTACTTGCT CATTGCTCAG CAAATGATGG AAGCTGATGG TGTTCCTTGT 
ATCAACCTGA AGGGCAAGAA GAAGGATGTC GCTGACTATC TTGAAAACTT CAAGCAAAAT 
AGAGGCATGC GATTCAGGGA TGACAAAAAA CAGGCCAAGC CCAGAACACC TGCACAAATC 
AAAGCGGCAG AAACTAGAGC TGCAAAAGCA AAAGCGGTAA AGGCTACAAA GGCTACAAAG 
GCTGCGGAAG TGACCGGTGT CCCAGTTGAT GCTGATGCTA TTTCTGGTGG TGCGGCACCG 
GCTTCTTTTG AGCTAAGCAT TCGGGTTAAT CCAAATTCTC AAATCCCCCA AAAGAGAAAG 
AGGAAAGCTA TGGCTACAAA AGGTGCTTCG GTCGGCCACG ACGACGTGGT GAGCAGTACA 
CATGCCGATC TTGAGACCGA GCGGAGAGCT GAGCTTGATA CTGCTTTTAA TTCCGATCCT 
CCTGCGCCAA TTGCAACACA AACCCCTTAC TCAACTCATC TTGTGCCAAA TATAACAATA 
CAGCCCCAAT TAAACGCTGA GGATTATATG ATTACGACTA AATTGGGACA TGAGGTTGAT 
CATGCTGGAA ATATTATTTA CGCGGATCAG GATAACCCTA TTCAAGCTCG TGAACTCATT 
GATGATGAAA ATGCAGCTTA TGATGCTCCT CCGCCCTCAT TCCCTGTCTC AAATCCTACT 
CGATCTGCGA AAGAGACAAA AAGGGCTATA AAGGCATGGA TAGCTGGCAA CGAGAAGATG 
ATTCAATATC CACCACTTAA TTCTTCTGAG CTCGAAGACA TTAAGGAAGC CGCTGCTCCT 
GAAGCCCGCA TACGTAATGC TCTCGCAGAC GATGTGCCTG TAGATTGGAC TCGCGTTGGC 
AAGGAGGCGT TTGATCCTGA AGACTTTGCT GAGAACCCTG ATCCCACCGC TCGTATCTTG 
TTTCTCGACA TTGCCGACGA GTCTGCTGCC GGATCCAATT TAACTGTCGC TCAGCACATC 
ATCTTCTTGA CTCCTTACTT TGCCGAAAAG CAAAAGTACA ATTCTTCAAT GACTCAGGCA 
ATGGGTCGTG CAATTCGTCA AGGTCAAGCA CCAGGTAGCA CTGTGACTGT TCATCATCTA 
CTTGTTCAGG ATTCTGTCGA GACTGATATT GCTTTTCAAC TTATGGATGA TCAATTTCCA 
GGAAGGATCA ATGCAACGCA TTTACTTTCT AAACATAAAT CTAACATTGA ACCTGCCTTT 
TTCAACAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001554539.1
CDS	1..6972
Translation	MAKKRQKTSRPAPCVEDGSDLEDEDGNGGIDSDDQAPGLHDQKRRTDESKQWNTLSFAEGIDSSLPPLTTATEFMEILLERLLNGGFKDVIKHLNGRPLRVATACSGTEAPILFLNSFSEGLRKRGHDFRIEHVFSCEIDPAKQSYIRRNFHNVPILRDITEVFEWEGNPEKIGFMTTAFGRTYALPAPGTVDVIIVGTSCTSYSNLNNQKSTDLERDQTESSRTFRAYVLLVKYLKAKIAISENVLSGPFEAMIKMLGDHGYIGGSANLDSKHYGIPQTRQRGYLIALPESSLGPDEIEEYKLIKTWKIYYANMKVMCSRPASSDAEQWMEPSDSKALRSQVEFQVESRAVAKWDTCCARHFDYRHAQGFGTSKPITDWRSAGNYCHPDNWPRNLKGFVERVHDVTDVSHLRGICRGYDDRYLSRNIDLSQNVYVQHDNVKCGLLPCLTPNGVMFNTARGSKLTGEEYLRLQGIDTNSLELGGISQQDLRNLAGNAMTVTVVGSVTTFALLKYFSKLTPGDGVKIPAQDIVKQSFRGGEDMLNSYEDITVVRRPLTFGECVHLVESSAKYCYCEAQTDISRSSIVKCKNCGHVSCTNCGIKPRHAYEPLDLYIGPRGTKGITPRISPSSIEFNIAERLPMMVSISGLFASDCQVLSILEEVATGDIDHNQWAALRNSFLNCLQSIVKFQKPVRSFAWNFTWLSEHARLELSLSNDKIQWVLYALPPPTAAVNSSLRRYLTKFPLARTVPINASDDSSRTIFDCTWQIWQPKIRTAQATISYGGDLISTYESSIDLPAWKGDFIFSKKTIQVLDDHPAFGEINGVYIAHPECQQAFNSYHSKNGTAGGDNQIHFFFDHEGGTGDWNKHGFVFTYDQTRKEFGVCRQIIASLCTSWEQPIAKKLNGATYQYGDTVYKSLPLLKEGMSEVVEITSFGSWTTTPEIISPMATKLSDRSSMVVYEKFNSLNPKLLLETDCKNMLSAFSCKAYTNVIPPYWEPDHWINVTKQNGTQFFKGCYHLLARGLVTPGIDEADPPWIPCNKQGDSSCRCDDCAPPKPDMLWHLKRSGQRIQQTPFENPPAAAIFETLTKNAPPSMQQWVRFLRKDAFFQFEYQFLICPAVLCHQAAALLNLFPRPEIPVHLSWRFLPGAWGSSKLPTRMFSYFNTETLKMSEQPITFVPGKKLRNEQLKTLTWMVKQERCPPSFMEREIVEICQNDLAFRLEGRASRIVKHRAGLIAAAVGYGKTVLTFALMGRQRRMDRLWANNPNINGQIHVKATLVLVPTHLTIQWRDEGCSFIGYDAKDDARIITLRSFTDVRALTVEDVRRAELVICSSALFDNEGYLKLMAKNAGVVNLAKGATDRAKEAWYLNAQKERRELLAYLLERLPMEVDENDKAAPARNAKIIKNFNKKTFQKYKKNVADAGVNEPHIPSMRRKGAELVKYLMEEAKKDFKGKGKPRTNVLIDEADAEKTFEFHYFEDDCVDGLTGETLRNDKNLTSAERFLSYGPDASATWHSQRLQVAQDFCDTFIRYDAKKGNGQYNRLHRFTVMPFATAQLACYLIMRADVINKSYMMPRAKANTIRSSVKLEISKVYGKDTDGRLRLTKAASFFPEHARKDTKYKLKTIKDLKAAALNEINASKNYLRQQMELLDYLVTEKLVFKDAQLVLRYRNWETQVLAGYRYPDPDALLDILQVVKGVRRTKSSKTKDDFYYENPGPAYGRRLWPSQTETIMHNGRKTLGLIAEFKIGLIELNKTAMDLMQFRADYRYYRSVGQIDQAGSVTPAGSLTCNCCNKSTPLYEAVIQGSCGHILCRKCNETSSEGNCPIEHCAAQSLDDQKYLASSLYSSAGKFPERKEPGTKPDELSLFWNNPSVRVDKLTAMRSIFWKHDDGKNKFIVFSPLPEYLLIAQQMMEADGVPCINLKGKKKDVADYLENFKQNRGMRFRDDKKQAKPRTPAQIKAAETRAAKAKAVKATKATKAAEVTGVPVDADAISGGAAPASFELSIRVNPNSQIPQKRKRKAMATKGASVGHDDVVSSTHADLETERRAELDTAFNSDPPAPIATQTPYSTHLVPNITIQPQLNAEDYMITTKLGHEVDHAGNIIYADQDNPIQARELIDDENAAYDAPPPSFPVSNPTRSAKETKRAIKAWIAGNEKMIQYPPLNSSELEDIKEAAAPEARIRNALADDVPVDWTRVGKEAFDPEDFAENPDPTARILFLDIADESAAGSNLTVAQHIIFLTPYFAEKQKYNSSMTQAMGRAIRQGQAPGSTVTVHHLLVQDSVETDIAFQLMDDQFPGRINATHLLSKHKSNIEPAFFNR

Target ORF information:

RefSeq Version	XM_001554489.1
Organism	Botrytis cinerea B05.10
Definition	Botryotinia fuckeliana B05.10 hypothetical protein (BC1G_07127) partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001554489.1

ORF Insert Sequence:

ATGGCGAAGA AACGCCAGAA GACTTCGCGT CCAGCACCAT GTGTTGAAGA TGGATCCGAT 
TTAGAGGATG AAGATGGCAA TGGTGGTATT GATTCAGACG ATCAAGCACC AGGTCTTCAT 
GATCAAAAGA GGAGAACCGA TGAGAGTAAA CAGTGGAACA CCCTCAGCTT CGCGGAGGGA 
ATCGACTCAA GTCTTCCTCC ACTTACTACT GCCACGGAAT TTATGGAGAT CTTGCTTGAA 
AGATTGTTGA ATGGGGGATT CAAAGACGTT ATAAAGCACC TCAATGGTCG GCCTTTGCGC 
GTCGCCACCG CATGTTCCGG CACTGAAGCA CCCATCCTGT TTCTGAATTC TTTTTCCGAA 
GGATTAAGGA AAAGAGGTCA TGACTTCAGA ATTGAGCATG TCTTCTCATG TGAAATTGAT 
CCGGCTAAAC AATCTTACAT TCGTCGAAAC TTTCATAATG TTCCTATTCT ACGTGATATC 
ACAGAAGTCT TCGAGTGGGA GGGTAACCCT GAAAAGATAG GTTTTATGAC TACCGCTTTT 
GGTAGAACTT ATGCGCTTCC AGCACCGGGT ACAGTTGACG TTATCATCGT TGGTACTTCT 
TGCACCAGCT ATTCCAATTT GAACAACCAA AAGAGTACGG ACTTGGAGAG AGACCAAACT 
GAGTCATCGC GCACTTTCCG CGCATATGTT TTGCTTGTTA AGTATCTAAA AGCAAAGATC 
GCTATCAGCG AAAATGTCCT GAGTGGCCCT TTCGAGGCTA TGATTAAAAT GCTGGGCGAT 
CACGGTTACA TCGGTGGCTC AGCAAACCTC GACAGCAAGC ATTATGGCAT TCCCCAAACC 
CGCCAACGCG GTTATCTCAT TGCCCTCCCT GAATCCTCTC TGGGACCCGA TGAGATAGAA 
GAGTATAAAC TTATAAAGAC ATGGAAAATA TACTATGCTA ACATGAAAGT CATGTGTTCG 
CGCCCTGCTA GCAGTGACGC TGAGCAGTGG ATGGAGCCAT CTGATTCAAA GGCACTCCGG 
TCTCAGGTCG AATTTCAAGT TGAATCACGT GCGGTCGCAA AGTGGGATAC GTGCTGTGCG 
AGGCATTTTG ATTATCGCCA TGCACAGGGC TTCGGTACTT CTAAGCCTAT TACCGATTGG 
CGCAGTGCGG GCAACTATTG TCATCCTGAT AATTGGCCCC GTAATCTTAA AGGCTTTGTA 
GAACGTGTTC ACGACGTTAC TGATGTCAGC CATTTGCGTG GCATATGCCG AGGTTATGAT 
GACCGTTACC TCAGTCGAAA CATCGATTTG TCACAAAACG TTTATGTTCA GCACGATAAC 
GTGAAGTGCG GTCTATTGCC TTGCTTGACA CCAAACGGTG TCATGTTTAA TACTGCACGT 
GGTAGCAAGC TTACTGGTGA AGAATATCTT CGCCTCCAGG GAATTGATAC TAATAGTCTT 
GAACTTGGTG GTATTTCTCA ACAAGACTTG AGAAACCTTG CTGGCAATGC GATGACAGTC 
ACAGTGGTTG GAAGTGTAAC GACGTTCGCG CTTCTTAAGT ACTTCTCAAA GCTCACTCCA 
GGCGATGGCG TCAAAATTCC AGCCCAGGAC ATAGTGAAAC AGAGTTTCCG TGGCGGAGAA 
GACATGCTCA ACTCATATGA AGATATTACG GTTGTTCGGC GGCCTTTGAC TTTTGGAGAA 
TGTGTTCATC TCGTCGAGTC TTCTGCAAAA TACTGCTATT GCGAAGCACA AACTGATATA 
TCACGATCGT CAATTGTCAA ATGCAAGAAC TGTGGGCATG TTTCATGTAC TAACTGTGGG 
ATTAAGCCGC GTCATGCCTA TGAGCCTTTG GATCTCTACA TTGGCCCTCG AGGTACCAAG 
GGTATTACTC CTCGTATTTC GCCTTCGTCC ATCGAGTTCA ATATTGCTGA ACGTCTGCCA 
ATGATGGTTT CAATATCAGG GTTGTTCGCC TCGGACTGCC AAGTGTTATC GATTCTCGAA 
GAAGTTGCTA CTGGTGACAT TGATCATAAT CAGTGGGCTG CCTTGCGTAA TTCTTTTTTA 
AATTGCCTAC AATCCATAGT CAAGTTTCAG AAGCCGGTCC GATCTTTTGC TTGGAATTTT 
ACATGGCTTT CGGAGCACGC CAGATTGGAG TTATCTCTTA GCAACGACAA GATTCAATGG 
GTTCTGTACG CTTTGCCACC TCCTACGGCG GCTGTTAACA GTTCGTTGAG GAGATATCTT 
ACAAAATTTC CTCTCGCAAG AACAGTCCCT ATTAACGCGT CTGATGACAG CTCAAGAACC 
ATATTCGATT GCACATGGCA GATATGGCAG CCAAAGATTC GCACCGCTCA GGCGACTATC 
AGCTATGGAG GCGACCTCAT TTCGACTTAC GAAAGCTCAA TCGATCTTCC TGCCTGGAAA 
GGAGATTTTA TATTCTCAAA GAAAACCATC CAGGTCCTAG ATGATCATCC CGCGTTCGGG 
GAAATCAATG GTGTTTATAT AGCGCATCCG GAATGTCAAC AGGCTTTTAA TAGCTACCAT 
TCCAAGAATG GCACTGCCGG AGGAGATAAT CAAATACATT TTTTCTTCGA TCATGAAGGA 
GGCACAGGGG ACTGGAACAA GCATGGGTTT GTCTTCACTT ACGATCAAAC ACGCAAAGAA 
TTTGGAGTAT GCAGACAAAT CATTGCTTCG CTTTGCACCT CGTGGGAACA GCCCATAGCA 
AAAAAACTTA ATGGTGCTAC TTATCAATAT GGAGATACCG TGTACAAAAG TCTTCCCTTG 
CTAAAAGAAG GAATGAGTGA AGTTGTCGAG ATTACCTCCT TTGGGAGCTG GACCACAACA 
CCCGAAATCA TCAGTCCTAT GGCTACCAAG TTGTCTGACC GATCTTCTAT GGTTGTCTAC 
GAAAAGTTCA ACTCACTTAA TCCAAAGCTT CTTCTCGAGA CTGATTGCAA AAACATGCTT 
AGTGCATTTT CTTGCAAAGC ATATACGAAT GTTATACCTC CTTATTGGGA ACCAGATCAT 
TGGATCAACG TCACTAAGCA AAATGGTACC CAATTTTTTA AGGGCTGTTA CCATCTGTTA 
GCTCGTGGCT TGGTTACTCC AGGAATCGAT GAGGCCGATC CGCCTTGGAT TCCATGCAAC 
AAACAAGGCG ACTCTTCATG TCGGTGCGAC GATTGTGCAC CACCCAAGCC AGATATGCTA 
TGGCATTTGA AGAGAAGCGG GCAAAGAATT CAGCAAACCC CATTCGAAAA TCCACCAGCA 
GCTGCGATCT TTGAGACTTT AACAAAGAAT GCGCCGCCGT CAATGCAGCA ATGGGTCAGA 
TTCCTCAGGA AAGATGCCTT CTTTCAATTC GAGTATCAAT TCCTTATCTG TCCCGCGGTT 
TTGTGTCATC AAGCAGCTGC GCTTCTTAAT TTGTTTCCAC GCCCAGAGAT TCCTGTGCAC 
CTCTCGTGGC GTTTCCTTCC TGGAGCTTGG GGAAGCAGTA AATTACCAAC GCGAATGTTT 
TCATATTTCA ATACAGAAAC CTTAAAGATG TCAGAACAGC CAATTACATT TGTTCCAGGA 
AAGAAGCTCC GCAATGAGCA ACTCAAAACT CTGACATGGA TGGTAAAGCA AGAGAGGTGC 
CCACCGTCTT TCATGGAGAG AGAAATTGTT GAAATTTGTC AAAATGACTT GGCATTCAGA 
CTTGAAGGCC GAGCTTCTCG AATAGTAAAA CACCGTGCTG GACTAATTGC TGCTGCCGTT 
GGATATGGCA AGACGGTTCT TACTTTTGCT TTAATGGGCA GGCAGCGTCG TATGGATCGC 
CTATGGGCCA ACAACCCTAA TATCAATGGT CAAATTCATG TCAAAGCAAC GCTGGTTTTG 
GTTCCAACGC ATCTGACTAT ACAGTGGCGA GATGAGGGAT GCTCGTTCAT TGGTTATGAT 
GCTAAGGATG ATGCAAGAAT TATAACACTC AGATCATTTA CAGACGTTAG GGCTCTGACT 
GTTGAAGATG TTCGGAGAGC AGAGCTAGTA ATTTGCAGCT CTGCCTTGTT CGATAATGAA 
GGATACTTGA AACTCATGGC GAAGAACGCT GGAGTTGTTA ATTTGGCCAA AGGCGCGACT 
GACAGAGCCA AGGAAGCTTG GTATCTTAAT GCTCAGAAAG AGAGACGGGA GCTCTTGGCG 
TATCTTTTGG AAAGGCTCCC AATGGAAGTA GATGAGAACG ACAAAGCAGC TCCTGCCCGA 
AACGCTAAAA TTATCAAGAA CTTCAACAAG AAGACTTTTC AGAAATACAA GAAAAATGTT 
GCGGATGCTG GGGTTAATGA GCCACATATT CCTTCAATGC GGCGTAAGGG CGCAGAACTT 
GTTAAATATC TCATGGAGGA AGCCAAGAAG GATTTCAAGG GCAAGGGCAA ACCCAGAACT 
AATGTCTTGA TTGATGAGGC AGATGCTGAG AAGACGTTCG AGTTCCACTA CTTTGAAGAT 
GATTGTGTTG ATGGCCTTAC TGGGGAGACA CTCCGCAATG ATAAGAATTT GACTAGCGCG 
GAAAGGTTTT TATCCTATGG ACCTGACGCA TCGGCAACCT GGCATTCTCA AAGACTCCAA 
GTTGCTCAAG ATTTTTGCGA TACTTTCATC CGCTACGACG CGAAAAAGGG AAATGGACAA 
TACAATCGCC TTCACCGTTT CACTGTAATG CCGTTTGCCA CAGCACAGTT GGCATGTTAC 
CTCATCATGA GAGCCGACGT TATTAACAAG AGCTACATGA TGCCAAGAGC TAAAGCTAAC 
ACCATTCGAA GTTCGGTCAA ATTGGAAATC TCAAAAGTTT ATGGTAAGGA TACAGACGGT 
CGACTGAGAT TAACCAAAGC CGCATCATTC TTCCCGGAGC ACGCTAGAAA GGATACAAAA 
TATAAACTTA AGACTATCAA AGACTTGAAA GCAGCCGCGC TCAATGAAAT CAATGCTTCG 
AAAAACTATT TGCGCCAACA GATGGAGCTA CTCGATTATT TGGTGACAGA GAAATTAGTC 
TTCAAGGATG CCCAACTGGT TCTTAGATAT AGGAACTGGG AAACTCAAGT TTTAGCCGGA 
TATCGTTACC CCGACCCCGA CGCTCTTCTT GATATCCTCC AAGTTGTCAA AGGCGTTCGA 
AGGACAAAGT CTAGTAAGAC CAAAGATGAC TTTTACTATG AAAATCCTGG TCCTGCTTAT 
GGTCGCCGTC TCTGGCCTTC TCAAACTGAG ACAATCATGC ACAACGGTCG CAAAACGCTT 
GGTCTAATTG CCGAATTTAA GATAGGGCTC ATCGAATTAA ACAAAACTGC TATGGACCTT 
ATGCAATTTA GAGCGGATTA TCGCTATTAT AGGTCTGTGG GGCAGATTGA CCAAGCTGGT 
AGCGTAACAC CTGCAGGAAG TCTCACTTGT AACTGTTGCA ACAAGAGTAC ACCTTTGTAC 
GAAGCAGTCA TTCAAGGTAG CTGTGGCCAT ATCCTATGTA GGAAATGCAA CGAAACCTCA 
TCGGAAGGCA ATTGCCCAAT TGAACACTGT GCTGCGCAAT CACTAGATGA CCAAAAGTAC 
TTGGCGTCAT CCTTATATTC TTCGGCTGGA AAATTTCCCG AGCGCAAAGA ACCAGGAACT 
AAACCCGACG AGCTTTCACT ATTCTGGAAT AACCCAAGTG TTCGAGTCGA CAAGCTGACA 
GCTATGCGGT CGATCTTCTG GAAGCACGAT GACGGCAAGA ATAAATTCAT TGTATTCAGT 
CCCCTACCAG AGTACTTGCT CATTGCTCAG CAAATGATGG AAGCTGATGG TGTTCCTTGT 
ATCAACCTGA AGGGCAAGAA GAAGGATGTC GCTGACTATC TTGAAAACTT CAAGCAAAAT 
AGAGGCATGC GATTCAGGGA TGACAAAAAA CAGGCCAAGC CCAGAACACC TGCACAAATC 
AAAGCGGCAG AAACTAGAGC TGCAAAAGCA AAAGCGGTAA AGGCTACAAA GGCTACAAAG 
GCTGCGGAAG TGACCGGTGT CCCAGTTGAT GCTGATGCTA TTTCTGGTGG TGCGGCACCG 
GCTTCTTTTG AGCTAAGCAT TCGGGTTAAT CCAAATTCTC AAATCCCCCA AAAGAGAAAG 
AGGAAAGCTA TGGCTACAAA AGGTGCTTCG GTCGGCCACG ACGACGTGGT GAGCAGTACA 
CATGCCGATC TTGAGACCGA GCGGAGAGCT GAGCTTGATA CTGCTTTTAA TTCCGATCCT 
CCTGCGCCAA TTGCAACACA AACCCCTTAC TCAACTCATC TTGTGCCAAA TATAACAATA 
CAGCCCCAAT TAAACGCTGA GGATTATATG ATTACGACTA AATTGGGACA TGAGGTTGAT 
CATGCTGGAA ATATTATTTA CGCGGATCAG GATAACCCTA TTCAAGCTCG TGAACTCATT 
GATGATGAAA ATGCAGCTTA TGATGCTCCT CCGCCCTCAT TCCCTGTCTC AAATCCTACT 
CGATCTGCGA AAGAGACAAA AAGGGCTATA AAGGCATGGA TAGCTGGCAA CGAGAAGATG 
ATTCAATATC CACCACTTAA TTCTTCTGAG CTCGAAGACA TTAAGGAAGC CGCTGCTCCT 
GAAGCCCGCA TACGTAATGC TCTCGCAGAC GATGTGCCTG TAGATTGGAC TCGCGTTGGC 
AAGGAGGCGT TTGATCCTGA AGACTTTGCT GAGAACCCTG ATCCCACCGC TCGTATCTTG 
TTTCTCGACA TTGCCGACGA GTCTGCTGCC GGATCCAATT TAACTGTCGC TCAGCACATC 
ATCTTCTTGA CTCCTTACTT TGCCGAAAAG CAAAAGTACA ATTCTTCAAT GACTCAGGCA 
ATGGGTCGTG CAATTCGTCA AGGTCAAGCA CCAGGTAGCA CTGTGACTGT TCATCATCTA 
CTTGTTCAGG ATTCTGTCGA GACTGATATT GCTTTTCAAC TTATGGATGA TCAATTTCCA 
GGAAGGATCA ATGCAACGCA TTTACTTTCT AAACATAAAT CTAACATTGA ACCTGCCTTT 
TTCAACAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BC1G_07127 ( XM_024694997.1 ) cDNA ORF clone, Botrytis cinerea B05.10 -> XP_024550790.1 Botrytis cinerea B05.10 hypothetical protein (BCIN_09g02510), mRNA.

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CloneID	OBc21195
Clone ID Related Accession (Same CDS sequence)	XM_024694997.1
Accession Version	XM_024694997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523808000000
Organism	Botrytis cinerea B05.10
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_037318).

ATGGCGAAGA AACGCCAGAA GACTTCGCGT CCAGCACCAT GTGTTGAAGA TGGATCCGAT 
TTAGAGGATG AAGATGGCAA TGGTGGTATT GATTCAGACG ATCAAGCACC AGGTCTTCAT 
GATCAAAAGA GGAGAACCGA TGAGAGTAAA CAGTGGAACA CCCTCAGCTT CGCGGAGGGA 
ATCGACTCAA GTCTTCCTCC ACTTACTACT GCCACGGAAT TTATGGAGAT CTTGCTTGAA 
AGATTGTTGA ATGGGGGATT CAAAGACGTT ATAAAGCACC TCAATGGTCG GCCTTTGCGC 
GTCGCCACCG CATGTTCCGG CACTGAAGCA CCCATCCTGT TTCTGAATTC TTTTTCCGAA 
GGATTAAGGA AAAGAGGTCA TGACTTCAGA ATTGAGCATG TCTTCTCATG TGAAATTGAT 
CCGGCTAAAC AATCTTACAT TCGTCGAAAC TTTCATAATG TTCCTATTCT ACGTGATATC 
ACAGAAGTCT TCGAGTGGGA GGGTAACCCT GAAAAGATAG GTTTTATGAC TACCGCTTTT 
GGTAGAACTT ATGCGCTTCC AGCACCGGGT ACAGTTGACG TTATCATCGT TGGTACTTCT 
TGCACCAGCT ATTCCAATTT GAACAACCAA AAGAGTACGG ACTTGGAGAG AGACCAAACT 
GAGTCATCGC GCACTTTCCG CGCATATGTT TTGCTTGTTA AGTATCTAAA AGCAAAGATC 
GCTATCAGCG AAAATGTCCT GAGTGGCCCT TTCGAGGCTA TGATTAAAAT GCTGGGCGAT 
CACGGTTACA TCGGTGGCTC AGCAAACCTC GACAGCAAGC ATTATGGCAT TCCCCAAACC 
CGCCAACGCG GTTATCTCAT TGCCCTCCCT GAATCCTCTC TGGGACCCGA TGAGATAGAA 
GAGTATAAAC TTATAAAGAC ATGGAAAATA TACTATGCTA ACATGAAAGT CATGTGTTCG 
CGCCCTGCTA GCAGTGACGC TGAGCAGTGG ATGGAGCCAT CTGATTCAAA GGCACTCCGG 
TCTCAGGTCG AATTTCAAGT TGAATCACGT GCGGTCGCAA AGTGGGATAC GTGCTGTGCG 
AGGCATTTTG ATTATCGCCA TGCACAGGGC TTCGGTACTT CTAAGCCTAT TACCGATTGG 
CGCAGTGCGG GCAACTATTG TCATCCTGAT AATTGGCCCC GTAATCTTAA AGGCTTTGTA 
GAACGTGTTC ACGACGTTAC TGATGTCAGC CATTTGCGTG GCATATGCCG AGGTTATGAT 
GACCGTTACC TCAGTCGAAA CATCGATTTG TCACAAAACG TTTATGTTCA GCACGATAAC 
GTGAAGTGCG GTCTATTGCC TTGCTTGACA CCAAACGGTG TCATGTTTAA TACTGCACGT 
GGTAGCAAGC TTACTGGTGA AGAATATCTT CGCCTCCAGG GAATTGATAC TAATAGTCTT 
GAACTTGGTG GTATTTCTCA ACAAGACTTG AGAAACCTTG CTGGCAATGC GATGACAGTC 
ACAGTGGTTG GAAGTGTAAC GACGTTCGCG CTTCTTAAGT ACTTCTCAAA GCTCACTCCA 
GGCGATGGCG TCAAAATTCC AGCCCAGGAC ATAGTGAAAC AGAGTTTCCG TGGCGGAGAA 
GACATGCTCA ACTCATATGA AGATATTACG GTTGTTCGGC GGCCTTTGAC TTTTGGAGAA 
TGTGTTCATC TCGTCGAGTC TTCTGCAAAA TACTGCTATT GCGAAGCACA AACTGATATA 
TCACGATCGT CAATTGTCAA ATGCAAGAAC TGTGGGCATG TTTCATGTAC TAACTGTGGG 
ATTAAGCCGC GTCATGCCTA TGAGCCTTTG GATCTCTACA TTGGCCCTCG AGGTACCAAG 
GGTATTACTC CTCGTATTTC GCCTTCGTCC ATCGAGTTCA ATATTGCTGA ACGTCTGCCA 
ATGATGGTTT CAATATCAGG GTTGTTCGCC TCGGACTGCC AAGTGTTATC GATTCTCGAA 
GAAGTTGCTA CTGGTGACAT TGATCATAAT CAGTGGGCTG CCTTGCGTAA TTCTTTTTTA 
AATTGCCTAC AATCCATAGT CAAGTTTCAG AAGCCGGTCC GATCTTTTGC TTGGAATTTT 
ACATGGCTTT CGGAGCACGC CAGATTGGAG TTATCTCTTA GCAACGACAA GATTCAATGG 
GTTCTGTACG CTTTGCCACC TCCTACGGCG GCTGTTAACA GTTCGTTGAG GAGATATCTT 
ACAAAATTTC CTCTCGCAAG AACAGTCCCT ATTAACGCGT CTGATGACAG CTCAAGAACC 
ATATTCGATT GCACATGGCA GATATGGCAG CCAAAGATTC GCACCGCTCA GGCGACTATC 
AGCTATGGAG GCGACCTCAT TTCGACTTAC GAAAGCTCAA TCGATCTTCC TGCCTGGAAA 
GGAGATTTTA TATTCTCAAA GAAAACCATC CAGGTCCTAG ATGATCATCC CGCGTTCGGG 
GAAATCAATG GTGTTTATAT AGCGCATCCG GAATGTCAAC AGGCTTTTAA TAGCTACCAT 
TCCAAGAATG GCACTGCCGG AGGAGATAAT CAAATACATT TTTTCTTCGA TCATGAAGGA 
GGCACAGGGG ACTGGAACAA GCATGGGTTT GTCTTCACTT ACGATCAAAC ACGCAAAGAA 
TTTGGAGTAT GCAGACAAAT CATTGCTTCG CTTTGCACCT CGTGGGAACA GCCCATAGCA 
AAAAAACTTA ATGGTGCTAC TTATCAATAT GGAGATACCG TGTACAAAAG TCTTCCCTTG 
CTAAAAGAAG GAATGAGTGA AGTTGTCGAG ATTACCTCCT TTGGGAGCTG GACCACAACA 
CCCGAAATCA TCAGTCCTAT GGCTACCAAG TTGTCTGACC GATCTTCTAT GGTTGTCTAC 
GAAAAGTTCA ACTCACTTAA TCCAAAGCTT CTTCTCGAGA CTGATTGCAA AAACATGCTT 
AGTGCATTTT CTTGCAAAGC ATATACGAAT GTTATACCTC CTTATTGGGA ACCAGATCAT 
TGGATCAACG TCACTAAGCA AAATGGTACC CAATTTTTTA AGGGCTGTTA CCATCTGTTA 
GCTCGTGGCT TGGTTACTCC AGGAATCGAT GAGGCCGATC CGCCTTGGAT TCCATGCAAC 
AAACAAGGCG ACTCTTCATG TCGGTGCGAC GATTGTGCAC CACCCAAGCC AGATATGCTA 
TGGCATTTGA AGAGAAGCGG GCAAAGAATT CAGCAAACCC CATTCGAAAA TCCACCAGCA 
GCTGCGATCT TTGAGACTTT AACAAAGAAT GCGCCGCCGT CAATGCAGCA ATGGGTCAGA 
TTCCTCAGGA AAGATGCCTT CTTTCAATTC GAGTATCAAT TCCTTATCTG TCCCGCGGTT 
TTGTGTCATC AAGCAGCTGC GCTTCTTAAT TTGTTTCCAC GCCCAGAGAT TCCTGTGCAC 
CTCTCGTGGC GTTTCCTTCC TGGAGCTTGG GGAAGCAGTA AATTACCAAC GCGAATGTTT 
TCATATTTCA ATACAGAAAC CTTAAAGATG TCAGAACAGC CAATTACATT TGTTCCAGGA 
AAGAAGCTCC GCAATGAGCA ACTCAAAACT CTGACATGGA TGGTAAAGCA AGAGAGGTGC 
CCACCGTCTT TCATGGAGAG AGAAATTGTT GAAATTTGTC AAAATGACTT GGCATTCAGA 
CTTGAAGGCC GAGCTTCTCG AATAGTAAAA CACCGTGCTG GACTAATTGC TGCTGCCGTT 
GGATATGGCA AGACGGTTCT TACTTTTGCT TTAATGGGCA GGCAGCGTCG TATGGATCGC 
CTATGGGCCA ACAACCCTAA TATCAATGGT CAAATTCATG TCAAAGCAAC GCTGGTTTTG 
GTTCCAACGC ATCTGACTAT ACAGTGGCGA GATGAGGGAT GCTCGTTCAT TGGTTATGAT 
GCTAAGGATG ATGCAAGAAT TATAACACTC AGATCATTTA CAGACGTTAG GGCTCTGACT 
GTTGAAGATG TTCGGAGAGC AGAGCTAGTA ATTTGCAGCT CTGCCTTGTT CGATAATGAA 
GGATACTTGA AACTCATGGC GAAGAACGCT GGAGTTGTTA ATTTGGCCAA AGGCGCGACT 
GACAGAGCCA AGGAAGCTTG GTATCTTAAT GCTCAGAAAG AGAGACGGGA GCTCTTGGCG 
TATCTTTTGG AAAGGCTCCC AATGGAAGTA GATGAGAACG ACAAAGCAGC TCCTGCCCGA 
AACGCTAAAA TTATCAAGAA CTTCAACAAG AAGACTTTTC AGAAATACAA GAAAAATGTT 
GCGGATGCTG GGGTTAATGA GCCACATATT CCTTCAATGC GGCGTAAGGG CGCAGAACTT 
GTTAAATATC TCATGGAGGA AGCCAAGAAG GATTTCAAGG GCAAGGGCAA ACCCAGAACT 
AATGTCTTGA TTGATGAGGC AGATGCTGAG AAGACGTTCG AGTTCCACTA CTTTGAAGAT 
GATTGTGTTG ATGGCCTTAC TGGGGTATGT TTGTCAGATT TTGCCTGGTC TCGGTGCGTC 
ATAGATGAAG TTGGCTACAT AACCCGCAGG AATATGCAGG TGGCTATATA TTCACTGACA 
GCTCAATCCC GGTGGGCCTT GTCAGCAACA CCTGATATTG TTGATTTCAA ATCGACTTCA 
AGAATTGCCC GGTGCCTTGG TATTAATCTC GGTGTCGACG ACCAGGAGAC ACTCCGCAAT 
GATAAGAATT TGACTAGCGC GGAAAGGTTT TTATCCTATG GACCTGACGC ATCGGCAACC 
TGGCATTCTC AAAGACTCCA AGTTGCTCAA GATTTTTGCG ATACTTTCAT CCGCTACGAC 
GCGAAAAAGG GAAATGGACA ATACAATCGC CTTCACCGTT TCACTGTAAT GCCGTTTGCC 
ACAGCACAGT TGGCATGTTA CCTCATCATG AGAGCCGACG TTATTAACAA GAGCTACATG 
ATGCCAAGAG CTAAAGCTAA CACCATTCGA AGTTCGGTCA AATTGGAAAT CTCAAAAGTT 
TATGGTAAGG ATACAGACGG TCGACTGAGA TTAACCAAAG CCGCATCATT CTTCCCGGAG 
CACGCTAGAA AGGATACAAA ATATAAACTT AAGACTATCA AAGACTTGAA AGCAGCCGCG 
CTCAATGAAA TCAATGCTTC GAAAAACTAT TTGCGCCAAC AGATGGAGCT ACTCGATTAT 
TTGGTGACAG AGAAATTAGT CTTCAAGGAT GCCCAACTGG TTCTTAGATA TAGGAACTGG 
GAAACTCAAG TTTTAGCCGG ATATCGTTAC CCCGACCCCG ACGCTCTTCT TGATATCCTC 
CAAGTTGTCA AAGGCGTTCG AAGGACAAAG TCTAGTAAGA CCAAAGATGA CTTTTACTAT 
GAAAATCCTG GTCCTGCTTA TGGTCGCCGT CTCTGGCCTT CTCAAACTGA GACAATCATG 
CACAACGGTC GCAAAACGCT TGGTCTAATT GCCGAATTTA AGATAGGGCT CATCGAATTA 
AACAAAACTG CTATGGACCT TATGCAATTT AGAGCGGATT ATCGCTATTA TAGGTCTGTG 
GGGCAGATTG ACCAAGCTGG TAGCGTAACA CCTGCAGGAA GTCTCACTTG TAACTGTTGC 
AACAAGAGTA CACCTTTGTA CGAAGCAGTC ATTCAAGGTA GCTGTGGCCA TATCCTATGT 
AGGAAATGCA ACGAAACCTC ATCGGAAGGC AATTGCCCAA TTGAACACTG TGCTGCGCAA 
TCACTAGATG ACCAAAAGTA CTTGGCGTCA TCCTTATATT CTTCGGCTGG AAAATTTCCC 
GAGCGCAAAG AACCAGGAAC TAAACCCGAC GAGCTTTCAC TATTCTGGAA TAACCCAAGT 
GTTCGAGTCG ACAAGCTGAC AGCTATGCGG TCGATCTTCT GGAAGCACGA TGACGGCAAG 
AATAAATTCA TTGTATTCAG TCCCCTACCA GAGTACTTGC TCATTGCTCA GCAAATGATG 
GAAGCTGATG GTGTTCCTTG TATCAACCTG AAGGGCAAGA AGAAGGATGT CGCTGACTAT 
CTTGAAAACT TCAAGCAAAA TAGAGGCATG CGATTCAGGG ATGACAAAAA ACAGGCCAAG 
CCCAGAACAC CTGCACAAAT CAAAGCGGCA GAAACTAGAG CTGCAAAAGC AAAAGCGGTA 
AAGGCTACAA AGGCTACAAA GGCTGCGGAA GTGACCGGTG TCCCAGTTGA TGCTGATGCT 
ATTTCTGGTG GTGCGGCACC GGCTTCTTTT GAGCTAAGCA TTCGGGTTAA TCCAAATTCT 
CAAATCCCCC AAAAGAGAAA GAGGAAAGCT ATGGCTACAA AAGGTAAAGC TGCAGAAGTT 
GAAGTAGAAT CTGTTGGTAT TGCACCAGTC GATAGTGACA CTACTCCGGT CGAATTCACG 
CCAGCTTCTG TCGAGACAAA CATTCAGGTC AAACCAGCCT TTCAAGGGCC CCAAAAAAGA 
AAAAGAAATG ATTCTGTCGA CAACACTCTA ATGGATCCGG TTTCCAAAGT TCGCCGTGTT 
ACCTTACCAG ATTCAGTTGA TCCAACGCCA GTTGCTTCAG AGTCCACACT TGTTTCAGAT 
TCTCAAGGTG CTTCGGTCGG CCACGACGAC GTGGTGAGCA GTACACATGC CGATCTTGAG 
ACCGAGCGGA GAGCTGAGCT TGATACTGCT TTTAATTCCG ATCCTCCTGC GCCAATTGCA 
ACACAAACCC CTTACTCAAC TCATCTTGTG CCAAATATAA CAATACAGCC CCAATTAAAC 
GCTGAGGATT ATATGATTAC GACTAAATTG GGACATGAGG TTGATCATGC TGGAAATATT 
ATTTACGCGG ATCAGGATAA CCCTATTCAA GCTCGTGAAC TCATTGATGA TGAAAATGCA 
GCTTATGATG CTCCTCCGCC CTCATTCCCT GTCTCAAATC CTACTCGATC TGCGAAAGAG 
ACAAAAAGGG CTATAAAGGC ATGGATAGCT GGCAACGAGA AGATGATTCA ATATCCACCA 
CTTAATTCTT CTGAGCTCGA AGACATTAAG GAAGCCGCTG CTCCTGAAGC CCGCATACGT 
AATGCTCTCG CAGACGATGT GCCTGTAGAT TGGACTCGCG TTGGCAAGGA GGCGTTTGAT 
CCTGAAGACT TTGCTGAGAA CCCTGATCCC ACCGCTCGTA TCTTGTTTCT CGACATTGCC 
GACGAGTCTG CTGCCGGATC CAATTTAACT GTCGCTCAGC ACATCATCTT CTTGACTCCT 
TACTTTGCCG AAAAGCAAAA GTACAATTCT TCAATGACTC AGGCAATGGG TCGTGCAATT 
CGTCAAGGTC AAGCACCAGG TAGCACTGTG ACTGTTCATC ATCTACTTGT TCAGGATTCT 
GTCGAGACTG ATATTGCTTT TCAACTTATG GATGATCAAT TTCCAGGAAG GATCAATGCA 
ACGCATTTAC TTTCTAAACA TAAATCTAAC ATTGAACCTG CCTTTTTCAA CAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024550790.1
CDS	990..8426
Translation	MAKKRQKTSRPAPCVEDGSDLEDEDGNGGIDSDDQAPGLHDQKRRTDESKQWNTLSFAEGIDSSLPPLTTATEFMEILLERLLNGGFKDVIKHLNGRPLRVATACSGTEAPILFLNSFSEGLRKRGHDFRIEHVFSCEIDPAKQSYIRRNFHNVPILRDITEVFEWEGNPEKIGFMTTAFGRTYALPAPGTVDVIIVGTSCTSYSNLNNQKSTDLERDQTESSRTFRAYVLLVKYLKAKIAISENVLSGPFEAMIKMLGDHGYIGGSANLDSKHYGIPQTRQRGYLIALPESSLGPDEIEEYKLIKTWKIYYANMKVMCSRPASSDAEQWMEPSDSKALRSQVEFQVESRAVAKWDTCCARHFDYRHAQGFGTSKPITDWRSAGNYCHPDNWPRNLKGFVERVHDVTDVSHLRGICRGYDDRYLSRNIDLSQNVYVQHDNVKCGLLPCLTPNGVMFNTARGSKLTGEEYLRLQGIDTNSLELGGISQQDLRNLAGNAMTVTVVGSVTTFALLKYFSKLTPGDGVKIPAQDIVKQSFRGGEDMLNSYEDITVVRRPLTFGECVHLVESSAKYCYCEAQTDISRSSIVKCKNCGHVSCTNCGIKPRHAYEPLDLYIGPRGTKGITPRISPSSIEFNIAERLPMMVSISGLFASDCQVLSILEEVATGDIDHNQWAALRNSFLNCLQSIVKFQKPVRSFAWNFTWLSEHARLELSLSNDKIQWVLYALPPPTAAVNSSLRRYLTKFPLARTVPINASDDSSRTIFDCTWQIWQPKIRTAQATISYGGDLISTYESSIDLPAWKGDFIFSKKTIQVLDDHPAFGEINGVYIAHPECQQAFNSYHSKNGTAGGDNQIHFFFDHEGGTGDWNKHGFVFTYDQTRKEFGVCRQIIASLCTSWEQPIAKKLNGATYQYGDTVYKSLPLLKEGMSEVVEITSFGSWTTTPEIISPMATKLSDRSSMVVYEKFNSLNPKLLLETDCKNMLSAFSCKAYTNVIPPYWEPDHWINVTKQNGTQFFKGCYHLLARGLVTPGIDEADPPWIPCNKQGDSSCRCDDCAPPKPDMLWHLKRSGQRIQQTPFENPPAAAIFETLTKNAPPSMQQWVRFLRKDAFFQFEYQFLICPAVLCHQAAALLNLFPRPEIPVHLSWRFLPGAWGSSKLPTRMFSYFNTETLKMSEQPITFVPGKKLRNEQLKTLTWMVKQERCPPSFMEREIVEICQNDLAFRLEGRASRIVKHRAGLIAAAVGYGKTVLTFALMGRQRRMDRLWANNPNINGQIHVKATLVLVPTHLTIQWRDEGCSFIGYDAKDDARIITLRSFTDVRALTVEDVRRAELVICSSALFDNEGYLKLMAKNAGVVNLAKGATDRAKEAWYLNAQKERRELLAYLLERLPMEVDENDKAAPARNAKIIKNFNKKTFQKYKKNVADAGVNEPHIPSMRRKGAELVKYLMEEAKKDFKGKGKPRTNVLIDEADAEKTFEFHYFEDDCVDGLTGVCLSDFAWSRCVIDEVGYITRRNMQVAIYSLTAQSRWALSATPDIVDFKSTSRIARCLGINLGVDDQETLRNDKNLTSAERFLSYGPDASATWHSQRLQVAQDFCDTFIRYDAKKGNGQYNRLHRFTVMPFATAQLACYLIMRADVINKSYMMPRAKANTIRSSVKLEISKVYGKDTDGRLRLTKAASFFPEHARKDTKYKLKTIKDLKAAALNEINASKNYLRQQMELLDYLVTEKLVFKDAQLVLRYRNWETQVLAGYRYPDPDALLDILQVVKGVRRTKSSKTKDDFYYENPGPAYGRRLWPSQTETIMHNGRKTLGLIAEFKIGLIELNKTAMDLMQFRADYRYYRSVGQIDQAGSVTPAGSLTCNCCNKSTPLYEAVIQGSCGHILCRKCNETSSEGNCPIEHCAAQSLDDQKYLASSLYSSAGKFPERKEPGTKPDELSLFWNNPSVRVDKLTAMRSIFWKHDDGKNKFIVFSPLPEYLLIAQQMMEADGVPCINLKGKKKDVADYLENFKQNRGMRFRDDKKQAKPRTPAQIKAAETRAAKAKAVKATKATKAAEVTGVPVDADAISGGAAPASFELSIRVNPNSQIPQKRKRKAMATKGKAAEVEVESVGIAPVDSDTTPVEFTPASVETNIQVKPAFQGPQKRKRNDSVDNTLMDPVSKVRRVTLPDSVDPTPVASESTLVSDSQGASVGHDDVVSSTHADLETERRAELDTAFNSDPPAPIATQTPYSTHLVPNITIQPQLNAEDYMITTKLGHEVDHAGNIIYADQDNPIQARELIDDENAAYDAPPPSFPVSNPTRSAKETKRAIKAWIAGNEKMIQYPPLNSSELEDIKEAAAPEARIRNALADDVPVDWTRVGKEAFDPEDFAENPDPTARILFLDIADESAAGSNLTVAQHIIFLTPYFAEKQKYNSSMTQAMGRAIRQGQAPGSTVTVHHLLVQDSVETDIAFQLMDDQFPGRINATHLLSKHKSNIEPAFFNR

Target ORF information:

RefSeq Version	XM_024694997.1
Organism	Botrytis cinerea B05.10
Definition	Botrytis cinerea B05.10 hypothetical protein (BCIN_09g02510), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024694997.1

ORF Insert Sequence:

ATGGCGAAGA AACGCCAGAA GACTTCGCGT CCAGCACCAT GTGTTGAAGA TGGATCCGAT 
TTAGAGGATG AAGATGGCAA TGGTGGTATT GATTCAGACG ATCAAGCACC AGGTCTTCAT 
GATCAAAAGA GGAGAACCGA TGAGAGTAAA CAGTGGAACA CCCTCAGCTT CGCGGAGGGA 
ATCGACTCAA GTCTTCCTCC ACTTACTACT GCCACGGAAT TTATGGAGAT CTTGCTTGAA 
AGATTGTTGA ATGGGGGATT CAAAGACGTT ATAAAGCACC TCAATGGTCG GCCTTTGCGC 
GTCGCCACCG CATGTTCCGG CACTGAAGCA CCCATCCTGT TTCTGAATTC TTTTTCCGAA 
GGATTAAGGA AAAGAGGTCA TGACTTCAGA ATTGAGCATG TCTTCTCATG TGAAATTGAT 
CCGGCTAAAC AATCTTACAT TCGTCGAAAC TTTCATAATG TTCCTATTCT ACGTGATATC 
ACAGAAGTCT TCGAGTGGGA GGGTAACCCT GAAAAGATAG GTTTTATGAC TACCGCTTTT 
GGTAGAACTT ATGCGCTTCC AGCACCGGGT ACAGTTGACG TTATCATCGT TGGTACTTCT 
TGCACCAGCT ATTCCAATTT GAACAACCAA AAGAGTACGG ACTTGGAGAG AGACCAAACT 
GAGTCATCGC GCACTTTCCG CGCATATGTT TTGCTTGTTA AGTATCTAAA AGCAAAGATC 
GCTATCAGCG AAAATGTCCT GAGTGGCCCT TTCGAGGCTA TGATTAAAAT GCTGGGCGAT 
CACGGTTACA TCGGTGGCTC AGCAAACCTC GACAGCAAGC ATTATGGCAT TCCCCAAACC 
CGCCAACGCG GTTATCTCAT TGCCCTCCCT GAATCCTCTC TGGGACCCGA TGAGATAGAA 
GAGTATAAAC TTATAAAGAC ATGGAAAATA TACTATGCTA ACATGAAAGT CATGTGTTCG 
CGCCCTGCTA GCAGTGACGC TGAGCAGTGG ATGGAGCCAT CTGATTCAAA GGCACTCCGG 
TCTCAGGTCG AATTTCAAGT TGAATCACGT GCGGTCGCAA AGTGGGATAC GTGCTGTGCG 
AGGCATTTTG ATTATCGCCA TGCACAGGGC TTCGGTACTT CTAAGCCTAT TACCGATTGG 
CGCAGTGCGG GCAACTATTG TCATCCTGAT AATTGGCCCC GTAATCTTAA AGGCTTTGTA 
GAACGTGTTC ACGACGTTAC TGATGTCAGC CATTTGCGTG GCATATGCCG AGGTTATGAT 
GACCGTTACC TCAGTCGAAA CATCGATTTG TCACAAAACG TTTATGTTCA GCACGATAAC 
GTGAAGTGCG GTCTATTGCC TTGCTTGACA CCAAACGGTG TCATGTTTAA TACTGCACGT 
GGTAGCAAGC TTACTGGTGA AGAATATCTT CGCCTCCAGG GAATTGATAC TAATAGTCTT 
GAACTTGGTG GTATTTCTCA ACAAGACTTG AGAAACCTTG CTGGCAATGC GATGACAGTC 
ACAGTGGTTG GAAGTGTAAC GACGTTCGCG CTTCTTAAGT ACTTCTCAAA GCTCACTCCA 
GGCGATGGCG TCAAAATTCC AGCCCAGGAC ATAGTGAAAC AGAGTTTCCG TGGCGGAGAA 
GACATGCTCA ACTCATATGA AGATATTACG GTTGTTCGGC GGCCTTTGAC TTTTGGAGAA 
TGTGTTCATC TCGTCGAGTC TTCTGCAAAA TACTGCTATT GCGAAGCACA AACTGATATA 
TCACGATCGT CAATTGTCAA ATGCAAGAAC TGTGGGCATG TTTCATGTAC TAACTGTGGG 
ATTAAGCCGC GTCATGCCTA TGAGCCTTTG GATCTCTACA TTGGCCCTCG AGGTACCAAG 
GGTATTACTC CTCGTATTTC GCCTTCGTCC ATCGAGTTCA ATATTGCTGA ACGTCTGCCA 
ATGATGGTTT CAATATCAGG GTTGTTCGCC TCGGACTGCC AAGTGTTATC GATTCTCGAA 
GAAGTTGCTA CTGGTGACAT TGATCATAAT CAGTGGGCTG CCTTGCGTAA TTCTTTTTTA 
AATTGCCTAC AATCCATAGT CAAGTTTCAG AAGCCGGTCC GATCTTTTGC TTGGAATTTT 
ACATGGCTTT CGGAGCACGC CAGATTGGAG TTATCTCTTA GCAACGACAA GATTCAATGG 
GTTCTGTACG CTTTGCCACC TCCTACGGCG GCTGTTAACA GTTCGTTGAG GAGATATCTT 
ACAAAATTTC CTCTCGCAAG AACAGTCCCT ATTAACGCGT CTGATGACAG CTCAAGAACC 
ATATTCGATT GCACATGGCA GATATGGCAG CCAAAGATTC GCACCGCTCA GGCGACTATC 
AGCTATGGAG GCGACCTCAT TTCGACTTAC GAAAGCTCAA TCGATCTTCC TGCCTGGAAA 
GGAGATTTTA TATTCTCAAA GAAAACCATC CAGGTCCTAG ATGATCATCC CGCGTTCGGG 
GAAATCAATG GTGTTTATAT AGCGCATCCG GAATGTCAAC AGGCTTTTAA TAGCTACCAT 
TCCAAGAATG GCACTGCCGG AGGAGATAAT CAAATACATT TTTTCTTCGA TCATGAAGGA 
GGCACAGGGG ACTGGAACAA GCATGGGTTT GTCTTCACTT ACGATCAAAC ACGCAAAGAA 
TTTGGAGTAT GCAGACAAAT CATTGCTTCG CTTTGCACCT CGTGGGAACA GCCCATAGCA 
AAAAAACTTA ATGGTGCTAC TTATCAATAT GGAGATACCG TGTACAAAAG TCTTCCCTTG 
CTAAAAGAAG GAATGAGTGA AGTTGTCGAG ATTACCTCCT TTGGGAGCTG GACCACAACA 
CCCGAAATCA TCAGTCCTAT GGCTACCAAG TTGTCTGACC GATCTTCTAT GGTTGTCTAC 
GAAAAGTTCA ACTCACTTAA TCCAAAGCTT CTTCTCGAGA CTGATTGCAA AAACATGCTT 
AGTGCATTTT CTTGCAAAGC ATATACGAAT GTTATACCTC CTTATTGGGA ACCAGATCAT 
TGGATCAACG TCACTAAGCA AAATGGTACC CAATTTTTTA AGGGCTGTTA CCATCTGTTA 
GCTCGTGGCT TGGTTACTCC AGGAATCGAT GAGGCCGATC CGCCTTGGAT TCCATGCAAC 
AAACAAGGCG ACTCTTCATG TCGGTGCGAC GATTGTGCAC CACCCAAGCC AGATATGCTA 
TGGCATTTGA AGAGAAGCGG GCAAAGAATT CAGCAAACCC CATTCGAAAA TCCACCAGCA 
GCTGCGATCT TTGAGACTTT AACAAAGAAT GCGCCGCCGT CAATGCAGCA ATGGGTCAGA 
TTCCTCAGGA AAGATGCCTT CTTTCAATTC GAGTATCAAT TCCTTATCTG TCCCGCGGTT 
TTGTGTCATC AAGCAGCTGC GCTTCTTAAT TTGTTTCCAC GCCCAGAGAT TCCTGTGCAC 
CTCTCGTGGC GTTTCCTTCC TGGAGCTTGG GGAAGCAGTA AATTACCAAC GCGAATGTTT 
TCATATTTCA ATACAGAAAC CTTAAAGATG TCAGAACAGC CAATTACATT TGTTCCAGGA 
AAGAAGCTCC GCAATGAGCA ACTCAAAACT CTGACATGGA TGGTAAAGCA AGAGAGGTGC 
CCACCGTCTT TCATGGAGAG AGAAATTGTT GAAATTTGTC AAAATGACTT GGCATTCAGA 
CTTGAAGGCC GAGCTTCTCG AATAGTAAAA CACCGTGCTG GACTAATTGC TGCTGCCGTT 
GGATATGGCA AGACGGTTCT TACTTTTGCT TTAATGGGCA GGCAGCGTCG TATGGATCGC 
CTATGGGCCA ACAACCCTAA TATCAATGGT CAAATTCATG TCAAAGCAAC GCTGGTTTTG 
GTTCCAACGC ATCTGACTAT ACAGTGGCGA GATGAGGGAT GCTCGTTCAT TGGTTATGAT 
GCTAAGGATG ATGCAAGAAT TATAACACTC AGATCATTTA CAGACGTTAG GGCTCTGACT 
GTTGAAGATG TTCGGAGAGC AGAGCTAGTA ATTTGCAGCT CTGCCTTGTT CGATAATGAA 
GGATACTTGA AACTCATGGC GAAGAACGCT GGAGTTGTTA ATTTGGCCAA AGGCGCGACT 
GACAGAGCCA AGGAAGCTTG GTATCTTAAT GCTCAGAAAG AGAGACGGGA GCTCTTGGCG 
TATCTTTTGG AAAGGCTCCC AATGGAAGTA GATGAGAACG ACAAAGCAGC TCCTGCCCGA 
AACGCTAAAA TTATCAAGAA CTTCAACAAG AAGACTTTTC AGAAATACAA GAAAAATGTT 
GCGGATGCTG GGGTTAATGA GCCACATATT CCTTCAATGC GGCGTAAGGG CGCAGAACTT 
GTTAAATATC TCATGGAGGA AGCCAAGAAG GATTTCAAGG GCAAGGGCAA ACCCAGAACT 
AATGTCTTGA TTGATGAGGC AGATGCTGAG AAGACGTTCG AGTTCCACTA CTTTGAAGAT 
GATTGTGTTG ATGGCCTTAC TGGGGTATGT TTGTCAGATT TTGCCTGGTC TCGGTGCGTC 
ATAGATGAAG TTGGCTACAT AACCCGCAGG AATATGCAGG TGGCTATATA TTCACTGACA 
GCTCAATCCC GGTGGGCCTT GTCAGCAACA CCTGATATTG TTGATTTCAA ATCGACTTCA 
AGAATTGCCC GGTGCCTTGG TATTAATCTC GGTGTCGACG ACCAGGAGAC ACTCCGCAAT 
GATAAGAATT TGACTAGCGC GGAAAGGTTT TTATCCTATG GACCTGACGC ATCGGCAACC 
TGGCATTCTC AAAGACTCCA AGTTGCTCAA GATTTTTGCG ATACTTTCAT CCGCTACGAC 
GCGAAAAAGG GAAATGGACA ATACAATCGC CTTCACCGTT TCACTGTAAT GCCGTTTGCC 
ACAGCACAGT TGGCATGTTA CCTCATCATG AGAGCCGACG TTATTAACAA GAGCTACATG 
ATGCCAAGAG CTAAAGCTAA CACCATTCGA AGTTCGGTCA AATTGGAAAT CTCAAAAGTT 
TATGGTAAGG ATACAGACGG TCGACTGAGA TTAACCAAAG CCGCATCATT CTTCCCGGAG 
CACGCTAGAA AGGATACAAA ATATAAACTT AAGACTATCA AAGACTTGAA AGCAGCCGCG 
CTCAATGAAA TCAATGCTTC GAAAAACTAT TTGCGCCAAC AGATGGAGCT ACTCGATTAT 
TTGGTGACAG AGAAATTAGT CTTCAAGGAT GCCCAACTGG TTCTTAGATA TAGGAACTGG 
GAAACTCAAG TTTTAGCCGG ATATCGTTAC CCCGACCCCG ACGCTCTTCT TGATATCCTC 
CAAGTTGTCA AAGGCGTTCG AAGGACAAAG TCTAGTAAGA CCAAAGATGA CTTTTACTAT 
GAAAATCCTG GTCCTGCTTA TGGTCGCCGT CTCTGGCCTT CTCAAACTGA GACAATCATG 
CACAACGGTC GCAAAACGCT TGGTCTAATT GCCGAATTTA AGATAGGGCT CATCGAATTA 
AACAAAACTG CTATGGACCT TATGCAATTT AGAGCGGATT ATCGCTATTA TAGGTCTGTG 
GGGCAGATTG ACCAAGCTGG TAGCGTAACA CCTGCAGGAA GTCTCACTTG TAACTGTTGC 
AACAAGAGTA CACCTTTGTA CGAAGCAGTC ATTCAAGGTA GCTGTGGCCA TATCCTATGT 
AGGAAATGCA ACGAAACCTC ATCGGAAGGC AATTGCCCAA TTGAACACTG TGCTGCGCAA 
TCACTAGATG ACCAAAAGTA CTTGGCGTCA TCCTTATATT CTTCGGCTGG AAAATTTCCC 
GAGCGCAAAG AACCAGGAAC TAAACCCGAC GAGCTTTCAC TATTCTGGAA TAACCCAAGT 
GTTCGAGTCG ACAAGCTGAC AGCTATGCGG TCGATCTTCT GGAAGCACGA TGACGGCAAG 
AATAAATTCA TTGTATTCAG TCCCCTACCA GAGTACTTGC TCATTGCTCA GCAAATGATG 
GAAGCTGATG GTGTTCCTTG TATCAACCTG AAGGGCAAGA AGAAGGATGT CGCTGACTAT 
CTTGAAAACT TCAAGCAAAA TAGAGGCATG CGATTCAGGG ATGACAAAAA ACAGGCCAAG 
CCCAGAACAC CTGCACAAAT CAAAGCGGCA GAAACTAGAG CTGCAAAAGC AAAAGCGGTA 
AAGGCTACAA AGGCTACAAA GGCTGCGGAA GTGACCGGTG TCCCAGTTGA TGCTGATGCT 
ATTTCTGGTG GTGCGGCACC GGCTTCTTTT GAGCTAAGCA TTCGGGTTAA TCCAAATTCT 
CAAATCCCCC AAAAGAGAAA GAGGAAAGCT ATGGCTACAA AAGGTAAAGC TGCAGAAGTT 
GAAGTAGAAT CTGTTGGTAT TGCACCAGTC GATAGTGACA CTACTCCGGT CGAATTCACG 
CCAGCTTCTG TCGAGACAAA CATTCAGGTC AAACCAGCCT TTCAAGGGCC CCAAAAAAGA 
AAAAGAAATG ATTCTGTCGA CAACACTCTA ATGGATCCGG TTTCCAAAGT TCGCCGTGTT 
ACCTTACCAG ATTCAGTTGA TCCAACGCCA GTTGCTTCAG AGTCCACACT TGTTTCAGAT 
TCTCAAGGTG CTTCGGTCGG CCACGACGAC GTGGTGAGCA GTACACATGC CGATCTTGAG 
ACCGAGCGGA GAGCTGAGCT TGATACTGCT TTTAATTCCG ATCCTCCTGC GCCAATTGCA 
ACACAAACCC CTTACTCAAC TCATCTTGTG CCAAATATAA CAATACAGCC CCAATTAAAC 
GCTGAGGATT ATATGATTAC GACTAAATTG GGACATGAGG TTGATCATGC TGGAAATATT 
ATTTACGCGG ATCAGGATAA CCCTATTCAA GCTCGTGAAC TCATTGATGA TGAAAATGCA 
GCTTATGATG CTCCTCCGCC CTCATTCCCT GTCTCAAATC CTACTCGATC TGCGAAAGAG 
ACAAAAAGGG CTATAAAGGC ATGGATAGCT GGCAACGAGA AGATGATTCA ATATCCACCA 
CTTAATTCTT CTGAGCTCGA AGACATTAAG GAAGCCGCTG CTCCTGAAGC CCGCATACGT 
AATGCTCTCG CAGACGATGT GCCTGTAGAT TGGACTCGCG TTGGCAAGGA GGCGTTTGAT 
CCTGAAGACT TTGCTGAGAA CCCTGATCCC ACCGCTCGTA TCTTGTTTCT CGACATTGCC 
GACGAGTCTG CTGCCGGATC CAATTTAACT GTCGCTCAGC ACATCATCTT CTTGACTCCT 
TACTTTGCCG AAAAGCAAAA GTACAATTCT TCAATGACTC AGGCAATGGG TCGTGCAATT 
CGTCAAGGTC AAGCACCAGG TAGCACTGTG ACTGTTCATC ATCTACTTGT TCAGGATTCT 
GTCGAGACTG ATATTGCTTT TCAACTTATG GATGATCAAT TTCCAGGAAG GATCAATGCA 
ACGCATTTAC TTTCTAAACA TAAATCTAAC ATTGAACCTG CCTTTTTCAA CAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A gapless genome sequence of the fungus Botrytis cinerea.
Molecular plant pathology18(1)75-89(2017 01)
Van Kan JA,Stassen JH,Mosbach A,Van Der Lee TA,Faino L,Farmer AD,Papasotiriou DG,Zhou S,Seidl MF,Cottam E,Edel D,Hahn M,Schwartz DC,Dietrich RA,Widdison S,Scalliet G

Genome update of Botrytis cinerea strains B05.10 and T4.
Eukaryotic cell11(11)1413-4(2012 Nov)
Staats M,van Kan JA

Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea.
PLoS genetics7(8)e1002230(2011 Aug)
Amselem J,Cuomo CA,van Kan JA,Viaud M,Benito EP,Couloux A,Coutinho PM,de Vries RP,Dyer PS,Fillinger S,Fournier E,Gout L,Hahn M,Kohn L,Lapalu N,Plummer KM,Pradier JM,Quévillon E,Sharon A,Simon A,ten Have A,Tudzynski B,Tudzynski P,Wincker P,Andrew M,Anthouard V,Beever RE,Beffa R,Benoit I,Bouzid O,Brault B,Chen Z,Choquer M,Collémare J,Cotton P,Danchin EG,Da Silva C,Gautier A,Giraud C,Giraud T,Gonzalez C,Grossetete S,Güldener U,Henrissat B,Howlett BJ,Kodira C,Kretschmer M,Lappartient A,Leroch M,Levis C,Mauceli E,Neuvéglise C,Oeser B,Pearson M,Poulain J,Poussereau N,Quesneville H,Rascle C,Schumacher J,Ségurens B,Sexton A,Silva E,Sirven C,Soanes DM,Talbot NJ,Templeton M,Yandava C,Yarden O,Zeng Q,Rollins JA,Lebrun MH,Dickman M

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BC1G_07127 cDNA ORF clone, Botrytis cinerea B05.10

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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