KCNU1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	KCNU1
Entrez Gene ID	524144
Full Name	potassium calcium-activated channel subfamily U member 1
General protein information	Preferred Names potassium calcium-activated channel subfamily U member 1 Names potassium channel subfamily U member 1 potassium channel, subfamily U, member 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001163249.1	NP_001156721.1	potassium channel subfamily U member 1
XM_024986272.1	XP_024842040.1	potassium channel subfamily U member 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	KCNU1	NP_001027006.2
Macaca mulatta (Rhesus monkey)	KCNU1	XP_001088318.1
Canis lupus familiaris (dog)	KCNU1	XP_539971.4
Bos taurus (cattle)	KCNU1	NP_001156721.1
Mus musculus (house mouse)	Kcnu1	NP_032458.3
Rattus norvegicus (Norway rat)	Kcnu1	XP_006253398.1
Pan troglodytes (chimpanzee)	KCNU1	XP_519704.4
Gallus gallus (chicken)	LOC100857499	XP_003642598.2

Gene Ontology
Bibliography

Related articles in PubMed

Bovine and mouse SLO3 K+ channels: evolutionary divergence points to an RCK1 region of critical function.
Santi CM, Butler A, Kuhn J, Wei A, Salkoff L
The Journal of biological chemistry284(32)21589-98(2009 Aug)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KCNU1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KCNU1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa36897	NM_001163249.1 Latest version!	Bos taurus potassium calcium-activated channel subfamily U member 1 (KCNU1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa231359	XM_024986272.1 Latest version!	Bos taurus potassium calcium-activated channel subfamily U member 1 (KCNU1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KCNU1 ( NM_001163249.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001156721.1 Bos taurus potassium calcium-activated channel subfamily U member 1 (KCNU1), mRNA.

Price & Availability↑

CloneID	OBa36897
Clone ID Related Accession (Same CDS sequence)	NM_001163249.1
Accession Version	NM_001163249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1508601600000
Organism	Bos taurus(cattle)
Product	potassium channel subfamily U member 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GQ214507.1. On Aug 8, 2009 this sequence version replaced XM_001787368.1. ##Evidence-Data-START## Transcript exon combination :: GQ214507.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145491 [ECO:0000348] ##Evidence-Data-END##

ATGTTTGTAA CCAAGCCAAA GGATGAACTT GAGGAAAGCA TGGAGAAATT GTCTTGTACA 
ACTGAGATCC AGGCAGTGTT TATTCTTTCC TCCATTGTAA TCTTCTTCTG TGGATTCCTC 
ATCTTATTCA TATCCAGGTT CATCGGGAGG AATTTTAAAA AATGGAAAAA GAGCAAGGGA 
ATTTTCCTGG AATTTAATTC AGGTAACATG AGACGTAAAC ATTCAAGAAG TCTCACCTTC 
CAGAGCCACT TTCGTGATCG TATAGAAATG CTGCTCTCGG CACAGACCTT GGTGGGACAG 
GTATTGGTTA TCCTTGTCTT TGTCCTAAGC ATCGGGTCTC TTATAATCTA TTTCATCAAC 
TCTACTGACC CAGTAAGAAG CTGTTCTTCA TACAAAGACA GAACCATCCC GATTGATTTG 
ACCTTCAACG CTTTCTTTAG TTTCTATTTT GGGCTGAGAT TTCTGGCAGC AGATGACAAG 
GTCAAGTTCT GGTTGGAGAT GAATTCGATT GTAGACATCT TTACCATCCC ACCCACCTTT 
ATTTCTTATT ACTTGAAGAG TAACTGGCTA GGTTTAAGGT TCTTACGAGC ATTGCGGCTG 
CTGGAACTCC CTCAAATCTT GCAAATTCTG CGAGCCATCA GCACCAGCAA TTCAGTGAAG 
TTTTGCAAGC TGTTATCCAT CGTTCTGAGC ATCTGGTTCA CGGCTGCAGG ATTCATTCAC 
CTGGTGGAAA ACTCCGGTGA TCCTTGGCTC CAAGGGAGAA ATTCACAGAC CATTTCATTC 
TTTGAGTCCA TTTACCTGAT CATGGCAACA ATGTCAACTG TCGGTTTTGG AGATGTGGTA 
CCCAAGACAT ACCTAGGACG GATTTTCATC ATAGTCTTCA CCCTCGGGAG TTTGGTACTG 
TTCGCCAACT ATGTCCCCGA AATGGTGGAA CTTCTTGCCA ACGAGAGGAA ATATACCAGT 
TCCTATGAAG TGGTCAAAGG GAAGAAGTTC ATCGTGGTGT GCGGAAACAT CACTGTGGAC 
AGTGTGATGG CTTTCCTGAG GAACTTTCTC CGCCACAAAG CAGGGGAGAT CAACACTGAG 
ATTGTATTCC TGGGAGAGGT CTCTCCTTCG TTGGAATTGG AAACCATATT TAAGCACCAC 
ATGGCCTATA CAACCTTCAT TTCTGGGTCT GCACTCAAGT GGGAGGATCT GAGGCGAGTT 
GCGGTGGACT CTGCAGAAGC GTGCCTGATC ATAGCCAACC CCCTATGCAG TGATTCACAT 
GCTGAAGACT CTTCAAACAT TATGAGGGTG CTCTCTATCA AGAACTATTA CCCTAACACC 
AGGATCATCA TACAGATACT TCAGTCCCAT AACAAGGCCT TTCTGCCAAA GATCCCCAGC 
TGGAACTGGA ACGCTGGCGA CAACATCATC TGCTTTGCTG AACTAAAGCT TGGATTCATC 
GCCCAGGGCT GCTTGGTGCC AGGCTTGTGT ACCTTCCTGA CGTCTCTATT TGTTGAGCAA 
AACAAGAAGA TCTGTCCTCA CGAGCCCTGG CAGCAGCTTT TCTGCAACGG CCTGAAGAAC 
AAAATCCTGA CGCAACGTCT CTCCAATGAC TTTGCTGGGA TGACCTTTCC TGACGCTTGC 
CAGCTCTGCT TCACAAAGAT GCACCTCATG CTGATCGCCA TCGAGTACAA GTCCGCCTGC 
CACGGTTACT GCGGGCTGAT ATTAAATCCA CCTGCTCAAG TTAAGCTCCG TAAGAACACA 
CTAGGGTTCT TTATCGCTGA ATCTGCAAAG GATGTCAAAA GAGCCTTCTT TTACTGTGCC 
ATCTGTCACA GCGATGTGCA GACTCCCGAG CTAATTGAAA AATGTGACTG CAAAAGCAAG 
GCTCGACGCG CGACAGGGAC AACGGTCGTG GTGATGAGAG ACAGCCAGGA GGAATACTGT 
GAACTCCAGG ACCCCTACTC AGAAACACGT ATGAGCTTCT ATAGCGGCTT GAGCGTTACT 
ACCAAGACTT TTTTCTGCGA AATGGAAGAG GAGTCTGACC GCCTCGATAG CAGCGGCATG 
TTTCACTGGT GTAGAGCCAC CCCTTTGAAC AAGATGATTC TGAAACGAAC TGACAAGGCA 
AAATACGAGT TTCGGGACCA CATTGTAGCA TGTATCTTTG GAGATGCCCA CTCAACACTG 
ATGGGACTTC GGAATTTTGT AATGCCATTG AGAGCTAGCA ACTATACCCG TCAGGAGCTG 
AAGGACATTG TGTTCATTGG GTCTCTGGAT TACCTGCAGA GAGAATGGCG ATTTATCCGG 
AATTTTCCCC AGATATACAT TCTGCCTGGA TCTGCACTCT ATTCCGGGGA CCTCCACGCG 
GTCAACATAG AGGAGTGTTC CATGTGTGCT GTCTTTTCCT CCTCCTGTAA GTCACCCAGT 
GGCCCGACTT TGGTGGACAC AGAGCCCATC ATGGCCACGC TCAACATCGG ATACCTGCGA 
ATTAGCTGCT CGGCCCGGAC ACCCTCAGGG CCACAGTTCG CCCAGTGCTT CAGTAGCAAT 
CCCAGGAAAT CAAAGTACCG AAGGATCCCC ATTCTCACCG AACTGAAAAA TCCTTCCAAC 
ATCCACTTTA TTGAACAGCT CAGTGGGATG GAATGGAACT TCTCCGGAAC GAACCTGCAT 
CTGAGCACTT CCTTCGCCAC TGGCACCGTC TTTTCTGGCA GCTTCTTAGA TTCTCTCCTG 
GCCACGGCTT TCTACAACTA CCATGTTGTA GAGTTACTTC AGATGCTAGT GACAGGAGGG 
ATGAATTTTC AGCTGGAACA ACATCTGGAT AAAGAAAAGT TACTTGGGCT GAATACCAGC 
TACTCTACGA TTTTACCTGG AAAGATGCGG TGTAAGCTGG GGCTGCTCTC CTTAAACCAG 
ACCGTCCTGT CAGACATTGA ACCAAGAAAA ACTTTTGGAC AAATATTCTG CGGCTCATTA 
AATAATCTTG GAATCCTGTG CCTCGGCTTA TACCGCATGA TAGATGAAGA AGAGCACAAT 
CCAGAGCACA AAAGGTTTGT GATTACCCGG CCAGAAAATG AGTTCAGCCT GCTGCCCTCG 
GATCTCGTGT TCTGCGCCAT CCCTTTCAGC ACCTCTTGTT ACAAAAAGGA TAGTAAAGCT 
TCAGCTCCAT ATGAGATTAT AAATATAATG CCACTGAAAA AAGAGACGGT CCCAGACGTA 
AGTCCTGACC AAACACCTCT AGTTGCCCAG GCCAGGGAGA GTGTATCACA ACTGCCCCAT 
AGGCCAGTCC CTTTGTTGGA GCCCAAACCA ATCCCTGCTA CTGATTCTAA GCAAGCAATA 
TTGACTCTGG GCAGTAAACA TTCATCTCAT CACCACTGCA CATACCCAGG TGAAACTGTG 
GAAGAAAATG GAAAAGAAAG CATGGAGGAA ACCACAGTGG AAGATGCCTA TGCCTTTCAC 
CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156721.1
CDS	1..3426
Translation	MFVTKPKDELEESMEKLSCTTEIQAVFILSSIVIFFCGFLILFISRFIGRNFKKWKKSKGIFLEFNSGNMRRKHSRSLTFQSHFRDRIEMLLSAQTLVGQVLVILVFVLSIGSLIIYFINSTDPVRSCSSYKDRTIPIDLTFNAFFSFYFGLRFLAADDKVKFWLEMNSIVDIFTIPPTFISYYLKSNWLGLRFLRALRLLELPQILQILRAISTSNSVKFCKLLSIVLSIWFTAAGFIHLVENSGDPWLQGRNSQTISFFESIYLIMATMSTVGFGDVVPKTYLGRIFIIVFTLGSLVLFANYVPEMVELLANERKYTSSYEVVKGKKFIVVCGNITVDSVMAFLRNFLRHKAGEINTEIVFLGEVSPSLELETIFKHHMAYTTFISGSALKWEDLRRVAVDSAEACLIIANPLCSDSHAEDSSNIMRVLSIKNYYPNTRIIIQILQSHNKAFLPKIPSWNWNAGDNIICFAELKLGFIAQGCLVPGLCTFLTSLFVEQNKKICPHEPWQQLFCNGLKNKILTQRLSNDFAGMTFPDACQLCFTKMHLMLIAIEYKSACHGYCGLILNPPAQVKLRKNTLGFFIAESAKDVKRAFFYCAICHSDVQTPELIEKCDCKSKARRATGTTVVVMRDSQEEYCELQDPYSETRMSFYSGLSVTTKTFFCEMEEESDRLDSSGMFHWCRATPLNKMILKRTDKAKYEFRDHIVACIFGDAHSTLMGLRNFVMPLRASNYTRQELKDIVFIGSLDYLQREWRFIRNFPQIYILPGSALYSGDLHAVNIEECSMCAVFSSSCKSPSGPTLVDTEPIMATLNIGYLRISCSARTPSGPQFAQCFSSNPRKSKYRRIPILTELKNPSNIHFIEQLSGMEWNFSGTNLHLSTSFATGTVFSGSFLDSLLATAFYNYHVVELLQMLVTGGMNFQLEQHLDKEKLLGLNTSYSTILPGKMRCKLGLLSLNQTVLSDIEPRKTFGQIFCGSLNNLGILCLGLYRMIDEEEHNPEHKRFVITRPENEFSLLPSDLVFCAIPFSTSCYKKDSKASAPYEIINIMPLKKETVPDVSPDQTPLVAQARESVSQLPHRPVPLLEPKPIPATDSKQAILTLGSKHSSHHHCTYPGETVEENGKESMEETTVEDAYAFHH

Target ORF information:

RefSeq Version	NM_001163249.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium calcium-activated channel subfamily U member 1 (KCNU1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163249.1

ORF Insert Sequence:

ATGTTTGTAA CCAAGCCAAA GGATGAACTT GAGGAAAGCA TGGAGAAATT GTCTTGTACA 
ACTGAGATCC AGGCAGTGTT TATTCTTTCC TCCATTGTAA TCTTCTTCTG TGGATTCCTC 
ATCTTATTCA TATCCAGGTT CATCGGGAGG AATTTTAAAA AATGGAAAAA GAGCAAGGGA 
ATTTTCCTGG AATTTAATTC AGGTAACATG AGACGTAAAC ATTCAAGAAG TCTCACCTTC 
CAGAGCCACT TTCGTGATCG TATAGAAATG CTGCTCTCGG CACAGACCTT GGTGGGACAG 
GTATTGGTTA TCCTTGTCTT TGTCCTAAGC ATCGGGTCTC TTATAATCTA TTTCATCAAC 
TCTACTGACC CAGTAAGAAG CTGTTCTTCA TACAAAGACA GAACCATCCC GATTGATTTG 
ACCTTCAACG CTTTCTTTAG TTTCTATTTT GGGCTGAGAT TTCTGGCAGC AGATGACAAG 
GTCAAGTTCT GGTTGGAGAT GAATTCGATT GTAGACATCT TTACCATCCC ACCCACCTTT 
ATTTCTTATT ACTTGAAGAG TAACTGGCTA GGTTTAAGGT TCTTACGAGC ATTGCGGCTG 
CTGGAACTCC CTCAAATCTT GCAAATTCTG CGAGCCATCA GCACCAGCAA TTCAGTGAAG 
TTTTGCAAGC TGTTATCCAT CGTTCTGAGC ATCTGGTTCA CGGCTGCAGG ATTCATTCAC 
CTGGTGGAAA ACTCCGGTGA TCCTTGGCTC CAAGGGAGAA ATTCACAGAC CATTTCATTC 
TTTGAGTCCA TTTACCTGAT CATGGCAACA ATGTCAACTG TCGGTTTTGG AGATGTGGTA 
CCCAAGACAT ACCTAGGACG GATTTTCATC ATAGTCTTCA CCCTCGGGAG TTTGGTACTG 
TTCGCCAACT ATGTCCCCGA AATGGTGGAA CTTCTTGCCA ACGAGAGGAA ATATACCAGT 
TCCTATGAAG TGGTCAAAGG GAAGAAGTTC ATCGTGGTGT GCGGAAACAT CACTGTGGAC 
AGTGTGATGG CTTTCCTGAG GAACTTTCTC CGCCACAAAG CAGGGGAGAT CAACACTGAG 
ATTGTATTCC TGGGAGAGGT CTCTCCTTCG TTGGAATTGG AAACCATATT TAAGCACCAC 
ATGGCCTATA CAACCTTCAT TTCTGGGTCT GCACTCAAGT GGGAGGATCT GAGGCGAGTT 
GCGGTGGACT CTGCAGAAGC GTGCCTGATC ATAGCCAACC CCCTATGCAG TGATTCACAT 
GCTGAAGACT CTTCAAACAT TATGAGGGTG CTCTCTATCA AGAACTATTA CCCTAACACC 
AGGATCATCA TACAGATACT TCAGTCCCAT AACAAGGCCT TTCTGCCAAA GATCCCCAGC 
TGGAACTGGA ACGCTGGCGA CAACATCATC TGCTTTGCTG AACTAAAGCT TGGATTCATC 
GCCCAGGGCT GCTTGGTGCC AGGCTTGTGT ACCTTCCTGA CGTCTCTATT TGTTGAGCAA 
AACAAGAAGA TCTGTCCTCA CGAGCCCTGG CAGCAGCTTT TCTGCAACGG CCTGAAGAAC 
AAAATCCTGA CGCAACGTCT CTCCAATGAC TTTGCTGGGA TGACCTTTCC TGACGCTTGC 
CAGCTCTGCT TCACAAAGAT GCACCTCATG CTGATCGCCA TCGAGTACAA GTCCGCCTGC 
CACGGTTACT GCGGGCTGAT ATTAAATCCA CCTGCTCAAG TTAAGCTCCG TAAGAACACA 
CTAGGGTTCT TTATCGCTGA ATCTGCAAAG GATGTCAAAA GAGCCTTCTT TTACTGTGCC 
ATCTGTCACA GCGATGTGCA GACTCCCGAG CTAATTGAAA AATGTGACTG CAAAAGCAAG 
GCTCGACGCG CGACAGGGAC AACGGTCGTG GTGATGAGAG ACAGCCAGGA GGAATACTGT 
GAACTCCAGG ACCCCTACTC AGAAACACGT ATGAGCTTCT ATAGCGGCTT GAGCGTTACT 
ACCAAGACTT TTTTCTGCGA AATGGAAGAG GAGTCTGACC GCCTCGATAG CAGCGGCATG 
TTTCACTGGT GTAGAGCCAC CCCTTTGAAC AAGATGATTC TGAAACGAAC TGACAAGGCA 
AAATACGAGT TTCGGGACCA CATTGTAGCA TGTATCTTTG GAGATGCCCA CTCAACACTG 
ATGGGACTTC GGAATTTTGT AATGCCATTG AGAGCTAGCA ACTATACCCG TCAGGAGCTG 
AAGGACATTG TGTTCATTGG GTCTCTGGAT TACCTGCAGA GAGAATGGCG ATTTATCCGG 
AATTTTCCCC AGATATACAT TCTGCCTGGA TCTGCACTCT ATTCCGGGGA CCTCCACGCG 
GTCAACATAG AGGAGTGTTC CATGTGTGCT GTCTTTTCCT CCTCCTGTAA GTCACCCAGT 
GGCCCGACTT TGGTGGACAC AGAGCCCATC ATGGCCACGC TCAACATCGG ATACCTGCGA 
ATTAGCTGCT CGGCCCGGAC ACCCTCAGGG CCACAGTTCG CCCAGTGCTT CAGTAGCAAT 
CCCAGGAAAT CAAAGTACCG AAGGATCCCC ATTCTCACCG AACTGAAAAA TCCTTCCAAC 
ATCCACTTTA TTGAACAGCT CAGTGGGATG GAATGGAACT TCTCCGGAAC GAACCTGCAT 
CTGAGCACTT CCTTCGCCAC TGGCACCGTC TTTTCTGGCA GCTTCTTAGA TTCTCTCCTG 
GCCACGGCTT TCTACAACTA CCATGTTGTA GAGTTACTTC AGATGCTAGT GACAGGAGGG 
ATGAATTTTC AGCTGGAACA ACATCTGGAT AAAGAAAAGT TACTTGGGCT GAATACCAGC 
TACTCTACGA TTTTACCTGG AAAGATGCGG TGTAAGCTGG GGCTGCTCTC CTTAAACCAG 
ACCGTCCTGT CAGACATTGA ACCAAGAAAA ACTTTTGGAC AAATATTCTG CGGCTCATTA 
AATAATCTTG GAATCCTGTG CCTCGGCTTA TACCGCATGA TAGATGAAGA AGAGCACAAT 
CCAGAGCACA AAAGGTTTGT GATTACCCGG CCAGAAAATG AGTTCAGCCT GCTGCCCTCG 
GATCTCGTGT TCTGCGCCAT CCCTTTCAGC ACCTCTTGTT ACAAAAAGGA TAGTAAAGCT 
TCAGCTCCAT ATGAGATTAT AAATATAATG CCACTGAAAA AAGAGACGGT CCCAGACGTA 
AGTCCTGACC AAACACCTCT AGTTGCCCAG GCCAGGGAGA GTGTATCACA ACTGCCCCAT 
AGGCCAGTCC CTTTGTTGGA GCCCAAACCA ATCCCTGCTA CTGATTCTAA GCAAGCAATA 
TTGACTCTGG GCAGTAAACA TTCATCTCAT CACCACTGCA CATACCCAGG TGAAACTGTG 
GAAGAAAATG GAAAAGAAAG CATGGAGGAA ACCACAGTGG AAGATGCCTA TGCCTTTCAC 
CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNU1 ( XM_024986272.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842040.1 Bos taurus potassium calcium-activated channel subfamily U member 1 (KCNU1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa231359
Clone ID Related Accession (Same CDS sequence)	XM_024986272.1
Accession Version	XM_024986272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	potassium channel subfamily U member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGTAA CCAAGCCAAA GGATGAACTT GAGGAAAGCA TGGAGAAATT GTCTTGTACA 
ACTGAGATCC AGGCAGTGTT TATTCTTTCC TCCATTGTAA TCTTCTTCTG TGGATTCCTC 
ATCTTATTCA TATCCAGGTT CATCGGGAGG AATTTTAAAA AATGGAAAAA GAGCAAGGGA 
ATTTTCCTGG AATTTAATTC AGGTAACATG AGACGTAAAC ATTCAAGAAG TCTCACCTTC 
CAGAGCCACT TTCGTGATCG TATAGAAATG CTGCTCTCGG CACAGACCTT GGTGGGACAG 
GTATTGGTTA TCCTTGTCTT TGTCCTAAGC ATCGGGTCTC TTATAATCTA TTTCATCAAC 
TCTACTGACC CAGTAAGAAG CTGTTCTTCA TACAAAGACA GAACCATCCC GATTGATTTG 
ACCTTCAACG CTTTCTTTAG TTTCTATTTT GGGCTGAGAT TTCTGGCAGC AGATGACAAG 
GTCAAGTTCT GGTTGGAGAT GAATTCGATT GTAGACATCT TTACCATCCC ACCCACCTTT 
ATTTCTTATT ACTTGAAGAG TAACTGGCTA GGTTTAAGGT TCTTACGAGC ATTGCGGCTG 
CTGGAACTCC CTCAAATCTT GCAAATTCTG CGAGCCATCA GCACCAGCAA TTCAGTGAAG 
TTTTGCAAGC TGTTATCCAT CGTTCTGAGC ATCTGGTTCA CGGCTGCAGG ATTCATTCAC 
CTGGTGGAAA ACTCCGGTGA TCCTTGGCTC CAAGGGAGAA ATTCACAGAC CATTTCATTC 
TTTGAGTCCA TTTACCTGAT CATGGCAACA ATGTCAACTG TCGGTTTTGG AGATGTGGTA 
CCCAAGACAT ACCTAGGACG GATTTTCATC ATAGTCTTCA CCCTCGGGAG TTTGGTACTG 
TTCGCCAACT ATGTCCCCGA AATGGTGGAA CTTCTTGCCA ACGAGAGGAA ATATACCAGT 
TCCTATGAAG TGGTCAAAGG GAAGAAGTTC ATCGTGGTGT GCGGAAACAT CACTGTGGAC 
AGTGTGATGG CTTTCCTGAG GAACTTTCTC CGCCACAAAG CAGGGGAGAT CAACACTGAG 
ATTGTATTCC TGGGAGAGGT CTCTCCTTCG TTGGAATTGG AAACCATATT TAAGCACCAC 
ATGGCCTATA CAACCTTCAT TTCTGGGTCT GCACTCAAGT GGGAGGATCT GAGGCGAGTT 
GCGGTGGACT CTGCAGAAGC GTGCCTGATC ATAGCCAACC CCCTATGCAG TGATTCACAT 
GCTGAAGACT CTTCAAACAT TATGAGGGTG CTCTCTATCA AGAACTATTA CCCTAACACC 
AGGATCATCA TACAGATACT TCAGTCCCAT AACAAGGCCT TTCTGCCAAA GATCCCCAGC 
TGGAACTGGA ACGCTGGCGA CAACATCATC TGCTTTGCTG AACTAAAGCT TGGATTCATC 
GCCCAGGGCT GCTTGGTGCC AGGCTTGTGT ACCTTCCTGA CGTCTCTATT TGTTGAGCAA 
AACAAGAAGA TCTGTCCTCA CGAGCCCTGG CAGCAGCTTT TCTGCAACGG CCTGAAGAAC 
AAAATCCTGA CGCAACGTCT CTCCAATGAC TTTGCTGGGA TGACCTTTCC TGACGCTTGC 
CAGCTCTGCT TCACAAAGAT GCACCTCATG CTGATCGCCA TCGAGTACAA GTCCGCCTGC 
CACGGTTACT GCGGGCTGAT ATTAAATCCA CCTGCTCAAG TTAAGCTCCG TAAGAACACA 
CTAGGGTTCT TTATCGCTGA ATCTGCAAAG GATGTCAAAA GAGCCTTCTT TTACTGTGCC 
ATCTGTCACA GCGATGTGCA GACTCCCGAG CTAATTGAAA AATGTGACTG CAAAAGCAAG 
GCTCGACGCG CGACAGGGAC AACGGTCGTG GTGATGAGAG ACAGCCAGGA GGAATACTGT 
GAACTCCAGG ACCCCTACTC AGAAACACGT ATGAGCTTCT ATAGCGGCTT GAGCGTTACT 
ACCAAGACTT TTTTCTGCGA AATGGAAGAG GAGTCTGACC GCCTCGATAG CAGCGGCATG 
TTTCACTGGT GTAGAGCCAC CCCTTTGAAC AAGATGATTC TGAAACGAAC TGACAAGGCA 
AAATACGAGT TTCGGGACCA CATTGTAGCA TGTATCTTTG GAGATGCCCA CTCAACACTG 
ATGGGACTTC GGAATTTTGT AATGCCATTG AGAGCTAGCA ACTATACCCG TCAGGAGCTG 
AAGGACATTG TGTTCATTGG GTCTCTGGAT TACCTGCAGA GAGAATGGCG ATTTATCCGG 
AATTTTCCCC AGATATACAT TCTGCCTGGA TCTGCACTCT ATTCCGGGGA CCTCCACGCG 
GTCAACATAG AGGAGTGTTC CATGTGTGCT GTCTTTTCCT CCTCCTGTAA GTCACCCAGT 
GGCCCGACTT TGGTGGACAC AGAGCCCATC ATGGCCACGC TCAACATCGG ATACCTGCGA 
ATTAGCTGCT CGGCCCGGAC ACCCTCAGGG CCACAGTTCG CCCAGTGCTT CAGTAGCAAT 
CCCAGGAAAT CAAAGTACCG AAGGATCCCC ATTCTCACCG AACTGAAAAA TCCTTCCAAC 
ATCCACTTTA TTGAACAGCT CAGTGGGATG GAATGGAACT TCTCCGGAAC GAACCTGCAT 
CTGAGCACTT CCTTCGCCAC TGGCACCGTC TTTTCTGGCA GCTTCTTAGA TTCTCTCCTG 
GCCACGGCTT TCTACAACTA CCATGTTGTA GAGTTACTTC AGATGCTAGT GACAGGAGGG 
ATGAATTTTC AGCTGGAACA ACATCTGGAT AAAGAAAAGT TACTTGGGCT GAATACCAGC 
TACTCTACGA TTTTACCTGG AAAGATGCGG TGTAAGCTGG GGCTGCTCTC CTTAAACCAG 
ACCGTCCTGT CAGACATTGA ACCAAGAAAA ACTTTTGGAC AAATATTCTG CGGCTCATTA 
AATAATCTTG GAATCCTGTG CCTCGGCTTA TACCGCATGA TAGATGAAGA AGAGCACAAT 
CCAGAGCACA AAAGGGAAGT ACGCTGGTTT GTGATTACCC GGCCAGAAAA TGAGTTCAGC 
CTGCTGCCCT CGGATCTCGT GTTCTGCGCC ATCCCTTTCA GCACCTCTTG TTACAAAAAG 
GATAGTAAAG CTTCAGCTCC ATATGAGATT ATAAATATAA TGCCACTGAA AAAAGAGACG 
GTCCCAGACG TAAGTCCTGA CCAAACACCT CTAGTTGCCC AGGCCAGGGA GAGTGTATCA 
CAACTGCCCC ATAGGCCAGT CCCTTTGTTG GAGCCCAAAC CAATCCCTGC TACTGATTCT 
AAGCAAGCAA TATTGACTCT GGGCAGTAAA CATTCATCTC ATCACCACTG CACATACCCA 
GGTGAAACTG TGGAAGAAAA TGGAAAAGAA AGCATGGAGG AAACCACAGT GGAAGATGCC 
TATGCCTTTC ACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842040.1
CDS	15..3452
Translation	MFVTKPKDELEESMEKLSCTTEIQAVFILSSIVIFFCGFLILFISRFIGRNFKKWKKSKGIFLEFNSGNMRRKHSRSLTFQSHFRDRIEMLLSAQTLVGQVLVILVFVLSIGSLIIYFINSTDPVRSCSSYKDRTIPIDLTFNAFFSFYFGLRFLAADDKVKFWLEMNSIVDIFTIPPTFISYYLKSNWLGLRFLRALRLLELPQILQILRAISTSNSVKFCKLLSIVLSIWFTAAGFIHLVENSGDPWLQGRNSQTISFFESIYLIMATMSTVGFGDVVPKTYLGRIFIIVFTLGSLVLFANYVPEMVELLANERKYTSSYEVVKGKKFIVVCGNITVDSVMAFLRNFLRHKAGEINTEIVFLGEVSPSLELETIFKHHMAYTTFISGSALKWEDLRRVAVDSAEACLIIANPLCSDSHAEDSSNIMRVLSIKNYYPNTRIIIQILQSHNKAFLPKIPSWNWNAGDNIICFAELKLGFIAQGCLVPGLCTFLTSLFVEQNKKICPHEPWQQLFCNGLKNKILTQRLSNDFAGMTFPDACQLCFTKMHLMLIAIEYKSACHGYCGLILNPPAQVKLRKNTLGFFIAESAKDVKRAFFYCAICHSDVQTPELIEKCDCKSKARRATGTTVVVMRDSQEEYCELQDPYSETRMSFYSGLSVTTKTFFCEMEEESDRLDSSGMFHWCRATPLNKMILKRTDKAKYEFRDHIVACIFGDAHSTLMGLRNFVMPLRASNYTRQELKDIVFIGSLDYLQREWRFIRNFPQIYILPGSALYSGDLHAVNIEECSMCAVFSSSCKSPSGPTLVDTEPIMATLNIGYLRISCSARTPSGPQFAQCFSSNPRKSKYRRIPILTELKNPSNIHFIEQLSGMEWNFSGTNLHLSTSFATGTVFSGSFLDSLLATAFYNYHVVELLQMLVTGGMNFQLEQHLDKEKLLGLNTSYSTILPGKMRCKLGLLSLNQTVLSDIEPRKTFGQIFCGSLNNLGILCLGLYRMIDEEEHNPEHKREVRWFVITRPENEFSLLPSDLVFCAIPFSTSCYKKDSKASAPYEIINIMPLKKETVPDVSPDQTPLVAQARESVSQLPHRPVPLLEPKPIPATDSKQAILTLGSKHSSHHHCTYPGETVEENGKESMEETTVEDAYAFHH

Target ORF information:

RefSeq Version	XM_024986272.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium calcium-activated channel subfamily U member 1 (KCNU1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986272.1

ORF Insert Sequence:

ATGTTTGTAA CCAAGCCAAA GGATGAACTT GAGGAAAGCA TGGAGAAATT GTCTTGTACA 
ACTGAGATCC AGGCAGTGTT TATTCTTTCC TCCATTGTAA TCTTCTTCTG TGGATTCCTC 
ATCTTATTCA TATCCAGGTT CATCGGGAGG AATTTTAAAA AATGGAAAAA GAGCAAGGGA 
ATTTTCCTGG AATTTAATTC AGGTAACATG AGACGTAAAC ATTCAAGAAG TCTCACCTTC 
CAGAGCCACT TTCGTGATCG TATAGAAATG CTGCTCTCGG CACAGACCTT GGTGGGACAG 
GTATTGGTTA TCCTTGTCTT TGTCCTAAGC ATCGGGTCTC TTATAATCTA TTTCATCAAC 
TCTACTGACC CAGTAAGAAG CTGTTCTTCA TACAAAGACA GAACCATCCC GATTGATTTG 
ACCTTCAACG CTTTCTTTAG TTTCTATTTT GGGCTGAGAT TTCTGGCAGC AGATGACAAG 
GTCAAGTTCT GGTTGGAGAT GAATTCGATT GTAGACATCT TTACCATCCC ACCCACCTTT 
ATTTCTTATT ACTTGAAGAG TAACTGGCTA GGTTTAAGGT TCTTACGAGC ATTGCGGCTG 
CTGGAACTCC CTCAAATCTT GCAAATTCTG CGAGCCATCA GCACCAGCAA TTCAGTGAAG 
TTTTGCAAGC TGTTATCCAT CGTTCTGAGC ATCTGGTTCA CGGCTGCAGG ATTCATTCAC 
CTGGTGGAAA ACTCCGGTGA TCCTTGGCTC CAAGGGAGAA ATTCACAGAC CATTTCATTC 
TTTGAGTCCA TTTACCTGAT CATGGCAACA ATGTCAACTG TCGGTTTTGG AGATGTGGTA 
CCCAAGACAT ACCTAGGACG GATTTTCATC ATAGTCTTCA CCCTCGGGAG TTTGGTACTG 
TTCGCCAACT ATGTCCCCGA AATGGTGGAA CTTCTTGCCA ACGAGAGGAA ATATACCAGT 
TCCTATGAAG TGGTCAAAGG GAAGAAGTTC ATCGTGGTGT GCGGAAACAT CACTGTGGAC 
AGTGTGATGG CTTTCCTGAG GAACTTTCTC CGCCACAAAG CAGGGGAGAT CAACACTGAG 
ATTGTATTCC TGGGAGAGGT CTCTCCTTCG TTGGAATTGG AAACCATATT TAAGCACCAC 
ATGGCCTATA CAACCTTCAT TTCTGGGTCT GCACTCAAGT GGGAGGATCT GAGGCGAGTT 
GCGGTGGACT CTGCAGAAGC GTGCCTGATC ATAGCCAACC CCCTATGCAG TGATTCACAT 
GCTGAAGACT CTTCAAACAT TATGAGGGTG CTCTCTATCA AGAACTATTA CCCTAACACC 
AGGATCATCA TACAGATACT TCAGTCCCAT AACAAGGCCT TTCTGCCAAA GATCCCCAGC 
TGGAACTGGA ACGCTGGCGA CAACATCATC TGCTTTGCTG AACTAAAGCT TGGATTCATC 
GCCCAGGGCT GCTTGGTGCC AGGCTTGTGT ACCTTCCTGA CGTCTCTATT TGTTGAGCAA 
AACAAGAAGA TCTGTCCTCA CGAGCCCTGG CAGCAGCTTT TCTGCAACGG CCTGAAGAAC 
AAAATCCTGA CGCAACGTCT CTCCAATGAC TTTGCTGGGA TGACCTTTCC TGACGCTTGC 
CAGCTCTGCT TCACAAAGAT GCACCTCATG CTGATCGCCA TCGAGTACAA GTCCGCCTGC 
CACGGTTACT GCGGGCTGAT ATTAAATCCA CCTGCTCAAG TTAAGCTCCG TAAGAACACA 
CTAGGGTTCT TTATCGCTGA ATCTGCAAAG GATGTCAAAA GAGCCTTCTT TTACTGTGCC 
ATCTGTCACA GCGATGTGCA GACTCCCGAG CTAATTGAAA AATGTGACTG CAAAAGCAAG 
GCTCGACGCG CGACAGGGAC AACGGTCGTG GTGATGAGAG ACAGCCAGGA GGAATACTGT 
GAACTCCAGG ACCCCTACTC AGAAACACGT ATGAGCTTCT ATAGCGGCTT GAGCGTTACT 
ACCAAGACTT TTTTCTGCGA AATGGAAGAG GAGTCTGACC GCCTCGATAG CAGCGGCATG 
TTTCACTGGT GTAGAGCCAC CCCTTTGAAC AAGATGATTC TGAAACGAAC TGACAAGGCA 
AAATACGAGT TTCGGGACCA CATTGTAGCA TGTATCTTTG GAGATGCCCA CTCAACACTG 
ATGGGACTTC GGAATTTTGT AATGCCATTG AGAGCTAGCA ACTATACCCG TCAGGAGCTG 
AAGGACATTG TGTTCATTGG GTCTCTGGAT TACCTGCAGA GAGAATGGCG ATTTATCCGG 
AATTTTCCCC AGATATACAT TCTGCCTGGA TCTGCACTCT ATTCCGGGGA CCTCCACGCG 
GTCAACATAG AGGAGTGTTC CATGTGTGCT GTCTTTTCCT CCTCCTGTAA GTCACCCAGT 
GGCCCGACTT TGGTGGACAC AGAGCCCATC ATGGCCACGC TCAACATCGG ATACCTGCGA 
ATTAGCTGCT CGGCCCGGAC ACCCTCAGGG CCACAGTTCG CCCAGTGCTT CAGTAGCAAT 
CCCAGGAAAT CAAAGTACCG AAGGATCCCC ATTCTCACCG AACTGAAAAA TCCTTCCAAC 
ATCCACTTTA TTGAACAGCT CAGTGGGATG GAATGGAACT TCTCCGGAAC GAACCTGCAT 
CTGAGCACTT CCTTCGCCAC TGGCACCGTC TTTTCTGGCA GCTTCTTAGA TTCTCTCCTG 
GCCACGGCTT TCTACAACTA CCATGTTGTA GAGTTACTTC AGATGCTAGT GACAGGAGGG 
ATGAATTTTC AGCTGGAACA ACATCTGGAT AAAGAAAAGT TACTTGGGCT GAATACCAGC 
TACTCTACGA TTTTACCTGG AAAGATGCGG TGTAAGCTGG GGCTGCTCTC CTTAAACCAG 
ACCGTCCTGT CAGACATTGA ACCAAGAAAA ACTTTTGGAC AAATATTCTG CGGCTCATTA 
AATAATCTTG GAATCCTGTG CCTCGGCTTA TACCGCATGA TAGATGAAGA AGAGCACAAT 
CCAGAGCACA AAAGGGAAGT ACGCTGGTTT GTGATTACCC GGCCAGAAAA TGAGTTCAGC 
CTGCTGCCCT CGGATCTCGT GTTCTGCGCC ATCCCTTTCA GCACCTCTTG TTACAAAAAG 
GATAGTAAAG CTTCAGCTCC ATATGAGATT ATAAATATAA TGCCACTGAA AAAAGAGACG 
GTCCCAGACG TAAGTCCTGA CCAAACACCT CTAGTTGCCC AGGCCAGGGA GAGTGTATCA 
CAACTGCCCC ATAGGCCAGT CCCTTTGTTG GAGCCCAAAC CAATCCCTGC TACTGATTCT 
AAGCAAGCAA TATTGACTCT GGGCAGTAAA CATTCATCTC ATCACCACTG CACATACCCA 
GGTGAAACTG TGGAAGAAAA TGGAAAAGAA AGCATGGAGG AAACCACAGT GGAAGATGCC 
TATGCCTTTC ACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Bovine and mouse SLO3 K+ channels: evolutionary divergence points to an RCK1 region of critical function.
The Journal of biological chemistry284(32)21589-98(2009 Aug)
Santi CM,Butler A,Kuhn J,Wei A,Salkoff L

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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KCNU1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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