NMT1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NMT1
Entrez Gene ID	281351
Full Name	N-myristoyltransferase 1
General protein information	Preferred Names N-myristoyltransferase 1 Names glycylpeptide N-tetradecanoyltransferase 1 NMT 1 myristoyl-CoA:protein N-myristoyltransferase 1 peptide N-myristoyltransferase 1 type I N-myristoyltransferase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_177504.2	NP_803470.2	glycylpeptide N-tetradecanoyltransferase 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nmt1	NP_683689.1
Drosophila melanogaster (fruit fly)	Nmt	NP_523969.1
Xenopus tropicalis (tropical clawed frog)	nmt2	XP_002935567.1
Pan troglodytes (chimpanzee)	NMT1	XP_001143871.1
Macaca mulatta (Rhesus monkey)	NMT1	XP_001115168.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001804	XP_321256.5
Mus musculus (house mouse)	Nmt1	NP_032733.1
Gallus gallus (chicken)	NMT1	XP_418088.1
Arabidopsis thaliana (thale cress)	NMT1	NP_568846.1
Danio rerio (zebrafish)	nmt1a	NP_001018316.1
Oryza sativa (rice)	Os01g0708100	NP_001044024.1
Homo sapiens (human)	NMT1	NP_066565.1
Canis lupus familiaris (dog)	NMT1	XP_537613.2
Bos taurus (cattle)	NMT1	NP_803470.2

Gene Ontology
Bibliography

Related articles in PubMed

Expression and activity of N-myristoyltransferase in lung inflammation of cattle and its role in neutrophil apoptosis.
Shrivastav A, Suri SS, Mohr R, Janardhan KS, Sharma RK, Singh B
Veterinary research41(1)9(2010 Jan-Feb)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Myristoyl-CoA:protein N-myristoyltransferases: isoform identification and gene expression in retina.
Rundle DR, Rajala RV, Alvarez RA, Anderson RE
Molecular vision10177-85(2004 Mar)

Molecular cloning and biochemical characterization of bovine spleen myristoyl CoA:protein N-myristoyltransferase.
Raju RV, Anderson JW, Datla RS, Sharma RK
Archives of biochemistry and biophysics348(1)134-42(1997 Dec)

Purification and partial sequencing of myristoyl-CoA:protein N-myristoyltransferase from bovine brain.
McIlhinney RA, McGlone K, Willis AC
The Biochemical journal290 ( Pt 2)405-10(1993 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NMT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NMT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa42154	NM_177504.2 Latest version!	Bos taurus N-myristoyltransferase 1 (NMT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NMT1 ( NM_177504.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_803470.2 Bos taurus N-myristoyltransferase 1 (NMT1), mRNA.

Price & Availability↑

CloneID	OBa42154
Clone ID Related Accession (Same CDS sequence)	NM_177504.2
Accession Version	NM_177504.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Bos taurus(cattle)
Product	glycylpeptide N-tetradecanoyltransferase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC109533.1. On Jan 26, 2006 this sequence version replaced NM_177504.1. ##Evidence-Data-START## Transcript exon combination :: BC109533.1, AF223384.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGACG AGAGTGATAC AGCAGTGAAG CCGCCGGCAC CTCCGCTGCC GCAGATGATG 
GAAGGGAATG GAAACGGCCA TGAGCACTGC AGCGATTGCG AGAACGAGGA GGACAACAGC 
TACAACCGGG GTGGTTTGAG TCCAGCCAAT GACACTGGAG CCAAAAAGAA GAAAAAGAAA 
CAAAAAAAGA AGAAAGAGAA AGGCAGCGAG ACAGATTCAG CCCAGGATCA GCCTGTGAAG 
ATGAACTCTT TGCCAGCAGA GAGGATCCAG GAAATACAGA AGGCTATTGA ACTTTTTTCA 
GTGGGTCAGG GACCTGCGAA AACCATGGAA GAGGCTAGCA AACGAAGCTA CCAGTTCTGG 
GACACGCAGC CTGTCCCCAA ATTGGGAGAA GTTGTGAACA CACATGGTCC TGTGGAGCCT 
GACAAAGACA ACATCCGCCA GGAGCCCTAC ACGCTGCCCC AGGGCTTCAC CTGGGACGCC 
TTGGACCTGG GGGACCGTGG TGTGCTGAAA GAACTCTACA CTCTCCTGAA TGAGAACTAT 
GTGGAAGATG ATGATAACAT GTTCCGGTTT GATTATTCCC CAGAATTTCT TTTGTGGGCT 
CTCCGGCCGC CTGGCTGGCT CCCCCAGTGG CACTGTGGGG TTCGAGTGGT CTCAAGTCGG 
AAACTAGGGG GGTTCATCAG CGCCATCCCG GCAAACATCC ATATCTACGA CACAGAGAAG 
AAGATGGTAG AGATCAACTT CCTGTGTGTC CACAAGAAGC TGCGCTCCAA GAGAGTGGCT 
CCAGTGCTGA TCCGTGAGAT AACCCGGCGG GTTCACCTGG AGGGCATCTT CCAAGCTGTT 
TACACTGCCG GGGTGGTGTT ACCAAAGCCT GTCGGCACCT GCAGGTACTG GCATCGGTCC 
CTAAATCCCC GGAAGCTGAT TGAAGTGAAG TTCTCCCACT TGAGCAGAAA CATGACCATG 
CAGCGCACCA TGAAGCTGTA CCGACTACCA GAGACTCCCA AGACAGCTGG GCTGCGACCA 
ATGGAGAAAA AGGACATTCC AGTAGTGCAC CAGCTCCTCA GCCGGTACCT GAAGCAGTTC 
CACCTCACGC CTGTCATGAG CCAGGAGGAG GTGGAGCACT GGTTCTACCC CCAGGAGAAT 
ATCATTGACA CTTTTGTGGT GGAGAATGCA AACGGGGAGG TGACTGATTT CCTGAGCTTT 
TATACACTTC CGTCCACCAT CATGAACCAC CCGACCCACA AGAGTCTGAA GGCTGCTTAC 
TCTTTCTACA ATGTTCACAC TCAGACCCCT CTCCTAGACC TCATGAGCGA CGCCCTCGTT 
CTCGCCAAAA TGAAAGGGTT TGACGTGTTC AATGCACTGG ATCTCATGGA GAACAAAACC 
TTCCTGGAGA AGCTCAAGTT CGGCATTGGG GATGGCAACC TACAGTATAA CCTGTACAAT 
TGGAAGTGCC CCAGCATGGG GGCAGAGAAG GTTGGGCTGG TGTTACAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_803470.2
CDS	223..1713
Translation	MADESDTAVKPPAPPLPQMMEGNGNGHEHCSDCENEEDNSYNRGGLSPANDTGAKKKKKKQKKKKEKGSETDSAQDQPVKMNSLPAERIQEIQKAIELFSVGQGPAKTMEEASKRSYQFWDTQPVPKLGEVVNTHGPVEPDKDNIRQEPYTLPQGFTWDALDLGDRGVLKELYTLLNENYVEDDDNMFRFDYSPEFLLWALRPPGWLPQWHCGVRVVSSRKLGGFISAIPANIHIYDTEKKMVEINFLCVHKKLRSKRVAPVLIREITRRVHLEGIFQAVYTAGVVLPKPVGTCRYWHRSLNPRKLIEVKFSHLSRNMTMQRTMKLYRLPETPKTAGLRPMEKKDIPVVHQLLSRYLKQFHLTPVMSQEEVEHWFYPQENIIDTFVVENANGEVTDFLSFYTLPSTIMNHPTHKSLKAAYSFYNVHTQTPLLDLMSDALVLAKMKGFDVFNALDLMENKTFLEKLKFGIGDGNLQYNLYNWKCPSMGAEKVGLVLQ

Target ORF information:

RefSeq Version	NM_177504.2
Organism	Bos taurus(cattle)
Definition	Bos taurus N-myristoyltransferase 1 (NMT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177504.2

ORF Insert Sequence:

ATGGCGGACG AGAGTGATAC AGCAGTGAAG CCGCCGGCAC CTCCGCTGCC GCAGATGATG 
GAAGGGAATG GAAACGGCCA TGAGCACTGC AGCGATTGCG AGAACGAGGA GGACAACAGC 
TACAACCGGG GTGGTTTGAG TCCAGCCAAT GACACTGGAG CCAAAAAGAA GAAAAAGAAA 
CAAAAAAAGA AGAAAGAGAA AGGCAGCGAG ACAGATTCAG CCCAGGATCA GCCTGTGAAG 
ATGAACTCTT TGCCAGCAGA GAGGATCCAG GAAATACAGA AGGCTATTGA ACTTTTTTCA 
GTGGGTCAGG GACCTGCGAA AACCATGGAA GAGGCTAGCA AACGAAGCTA CCAGTTCTGG 
GACACGCAGC CTGTCCCCAA ATTGGGAGAA GTTGTGAACA CACATGGTCC TGTGGAGCCT 
GACAAAGACA ACATCCGCCA GGAGCCCTAC ACGCTGCCCC AGGGCTTCAC CTGGGACGCC 
TTGGACCTGG GGGACCGTGG TGTGCTGAAA GAACTCTACA CTCTCCTGAA TGAGAACTAT 
GTGGAAGATG ATGATAACAT GTTCCGGTTT GATTATTCCC CAGAATTTCT TTTGTGGGCT 
CTCCGGCCGC CTGGCTGGCT CCCCCAGTGG CACTGTGGGG TTCGAGTGGT CTCAAGTCGG 
AAACTAGGGG GGTTCATCAG CGCCATCCCG GCAAACATCC ATATCTACGA CACAGAGAAG 
AAGATGGTAG AGATCAACTT CCTGTGTGTC CACAAGAAGC TGCGCTCCAA GAGAGTGGCT 
CCAGTGCTGA TCCGTGAGAT AACCCGGCGG GTTCACCTGG AGGGCATCTT CCAAGCTGTT 
TACACTGCCG GGGTGGTGTT ACCAAAGCCT GTCGGCACCT GCAGGTACTG GCATCGGTCC 
CTAAATCCCC GGAAGCTGAT TGAAGTGAAG TTCTCCCACT TGAGCAGAAA CATGACCATG 
CAGCGCACCA TGAAGCTGTA CCGACTACCA GAGACTCCCA AGACAGCTGG GCTGCGACCA 
ATGGAGAAAA AGGACATTCC AGTAGTGCAC CAGCTCCTCA GCCGGTACCT GAAGCAGTTC 
CACCTCACGC CTGTCATGAG CCAGGAGGAG GTGGAGCACT GGTTCTACCC CCAGGAGAAT 
ATCATTGACA CTTTTGTGGT GGAGAATGCA AACGGGGAGG TGACTGATTT CCTGAGCTTT 
TATACACTTC CGTCCACCAT CATGAACCAC CCGACCCACA AGAGTCTGAA GGCTGCTTAC 
TCTTTCTACA ATGTTCACAC TCAGACCCCT CTCCTAGACC TCATGAGCGA CGCCCTCGTT 
CTCGCCAAAA TGAAAGGGTT TGACGTGTTC AATGCACTGG ATCTCATGGA GAACAAAACC 
TTCCTGGAGA AGCTCAAGTT CGGCATTGGG GATGGCAACC TACAGTATAA CCTGTACAAT 
TGGAAGTGCC CCAGCATGGG GGCAGAGAAG GTTGGGCTGG TGTTACAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression and activity of N-myristoyltransferase in lung inflammation of cattle and its role in neutrophil apoptosis.
Veterinary research41(1)9(2010 Jan-Feb)
Shrivastav A,Suri SS,Mohr R,Janardhan KS,Sharma RK,Singh B

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Myristoyl-CoA:protein N-myristoyltransferases: isoform identification and gene expression in retina.
Molecular vision10177-85(2004 Mar)
Rundle DR,Rajala RV,Alvarez RA,Anderson RE

Molecular cloning and biochemical characterization of bovine spleen myristoyl CoA:protein N-myristoyltransferase.
Archives of biochemistry and biophysics348(1)134-42(1997 Dec)
Raju RV,Anderson JW,Datla RS,Sharma RK

Purification and partial sequencing of myristoyl-CoA:protein N-myristoyltransferase from bovine brain.
The Biochemical journal290 ( Pt 2)405-10(1993 Mar)
McIlhinney RA,McGlone K,Willis AC