NUC cDNA ORF clone, Bombyx mori(Domestic silkworm)

Gene
Clones
gRNAs

Summary

Gene Symbol	NUC
Entrez Gene ID	767621
Full Name	alkaline nuclease
Gene Type	protein-coding
Organism	Bombyx mori(Domestic silkworm)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001098274.1	NP_001091744.1	alkaline nuclease precursor
XM_012691673.2	XP_012547127.1	alkaline nuclease isoform X1
XM_012691673.1	XP_012547127.1	alkaline nuclease isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
Ou J, Deng HM, Zheng SC, Huang LH, Feng QL, Liu L
BMC genomics15820(2014 Sep)

Large scale full-length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori.
Suetsugu Y, Futahashi R, Kanamori H, Kadono-Okuda K, Sasanuma S, Narukawa J, Ajimura M, Jouraku A, Namiki N, Shimomura M, Sezutsu H, Osanai-Futahashi M, Suzuki MG, Daimon T, Shinoda T, Taniai K, Asaoka K, Niwa R, Kawaoka S, Katsuma S, Tamura T, Noda H, Kasahara M, Sugano S, Suzuki Y, Fujiwara H, Kataoka H, Arunkumar KP, Tomar A, Nagaraju J, Goldsmith MR, Feng Q, Xia Q, Yamamoto K, Shimada T, Mita K
G3 (Bethesda, Md.)3(9)1481-92(2013 Sep)

Bombyx mori DNA/RNA non-specific nuclease: expression of isoforms in insect culture cells, subcellular localization and functional assays.
Liu J, Swevers L, Iatrou K, Huvenne H, Smagghe G
Journal of insect physiology58(8)1166-76(2012 Aug)

Molecular characterization of a cDNA encoding extracellular dsRNase and its expression in the silkworm, Bombyx mori.
Arimatsu Y, Kotani E, Sugimura Y, Furusawa T
Insect biochemistry and molecular biology37(2)176-83(2007 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NUC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBg00823	NM_001098274.1 Latest version!	Bombyx mori alkaline nuclease (Nuc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBg14183	XM_012691673.2 Latest version!	Bombyx mori alkaline nuclease (Nuc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OBg14183	XM_012691673.1	Bombyx mori alkaline nuclease (Nuc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NUC ( NM_001098274.1 ) cDNA ORF clone, Bombyx mori(Domestic silkworm) -> NP_001091744.1 Bombyx mori alkaline nuclease (Nuc), mRNA.

Price & Availability↑

CloneID	OBg00823
Clone ID Related Accession (Same CDS sequence)	NM_001098274.1
Accession Version	NM_001098274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498233600000
Organism	Bombyx mori(domestic silkworm)
Product	alkaline nuclease precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB254196.1. ##Evidence-Data-START## Transcript exon combination :: AB254196.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00058752, SAMD00058753 [ECO:0000348] ##Evidence-Data-END##

ATGCGTCTGA CGCTTGTACT CGCAGCATTA GCTGTAGCGG TGGTGGCACT GCCCTCCAAA 
CTTCGAACGG ATCTACCAGA GCCAGGACAA CTGGCTTTTG TTCTTAACGA AGATGAGTTT 
GAAGACTACC TCGATGCCTA TCTCGCTCTT CAACAATCTG AAATGCTCGC CAATCAAACC 
AGGAATGATT TCCGAAGCGG TTGCACATTC CGAGTCAACG GTGATCTTGG TCAGCCTCAA 
CCTGTATATA TCCACAGAGG AAATTATTTA AGTCCAACTG GTAATACAGG TCAGATTCGT 
CTTAATCGTG GGGAACAAGT TCTTATCGCT TGCACAGGAT CCGGCCGTAC GATCCGTCAC 
CCCAATGTTG CTTCTAACCT TGCTGTCGGG ACGGTCAGCT GTCAAAACAA TAATCTCGTC 
ACAGCCAATT GGCTGCGTGG TAACAGTGCT TTCGGGCAAC TCACATGCTC TTCTCACGCT 
TACCATGACG CTCAACAAAC CAACACTAGA TGCTTCAATA ATCACTTTGT TATTCGTGTG 
GGATTCATCG TCAACAATGT ATTTTATCCT CTATATTGGT CCTGCTTTGA CCGCAACCGC 
TTAGAGGTTC TATATGTATG GTACCACCAA AACCCACCTA ATTCCGTATT CCAGTCTCGC 
GTTGATAGGC CTAGTTGGAT AGCTGGAAAC TTCTTCCCCG GTGTAGCTGT TAACTCTGCC 
TATACTCAAG TATCTCAAAG AAACATGATC GCTGGATTCG TGGGTAATGC CCTCGCAGAC 
AGATATGTAA CGTCCACGCA GTTCTTAGCA CGGGGTCATC TTGCTGCTAA GACTGACTTT 
ATATATGCAA CCGGTCAACG TGCTAGCTTC TACTTTATTA ACGCTGCTCC TCAATGGCAG 
CCTTTCAACG CTGGAAACTG GAACCGGCTC GAGCAGAATC TTCGTCGTAG AATTGGACAG 
GCTGGATATC ATACCATGGT CTACACTGGA ACATTCCGCG TAACACAACT TAGAAATCAA 
AACAACCGAC TTGTTGATAT TTTCTTGCAT CGTGCATCCA ACGGCGCTCT TCAAATTCCT 
GTACCACTTT ATTTCTACAA GGTGGTGCAG GACTCTTCTC GTCGCTTCGG TACCGCCTTT 
ATCAGTATCA ACAATCCTTA CTACACCCAA GCGGAGGCTC GCAATCTGCA GTTCTGCACA 
GACCGTTGCC GCAATAACAA CGCATTCAAC TGGGTGGGGT GGCAACCTGA TCGCATCGAC 
CTCGGCTATA GCTTCTGCTG TACCATTGCT GATTTCCGAA GAACTATTCC GCACCTTCCA 
GCTTTCAATG TTAACGGACT TCTGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091744.1
CDS	1..1350
Translation	MRLTLVLAALAVAVVALPSKLRTDLPEPGQLAFVLNEDEFEDYLDAYLALQQSEMLANQTRNDFRSGCTFRVNGDLGQPQPVYIHRGNYLSPTGNTGQIRLNRGEQVLIACTGSGRTIRHPNVASNLAVGTVSCQNNNLVTANWLRGNSAFGQLTCSSHAYHDAQQTNTRCFNNHFVIRVGFIVNNVFYPLYWSCFDRNRLEVLYVWYHQNPPNSVFQSRVDRPSWIAGNFFPGVAVNSAYTQVSQRNMIAGFVGNALADRYVTSTQFLARGHLAAKTDFIYATGQRASFYFINAAPQWQPFNAGNWNRLEQNLRRRIGQAGYHTMVYTGTFRVTQLRNQNNRLVDIFLHRASNGALQIPVPLYFYKVVQDSSRRFGTAFISINNPYYTQAEARNLQFCTDRCRNNNAFNWVGWQPDRIDLGYSFCCTIADFRRTIPHLPAFNVNGLLT

Target ORF information:

RefSeq Version	NM_001098274.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori alkaline nuclease (Nuc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098274.1

ORF Insert Sequence:

ATGCGTCTGA CGCTTGTACT CGCAGCATTA GCTGTAGCGG TGGTGGCACT GCCCTCCAAA 
CTTCGAACGG ATCTACCAGA GCCAGGACAA CTGGCTTTTG TTCTTAACGA AGATGAGTTT 
GAAGACTACC TCGATGCCTA TCTCGCTCTT CAACAATCTG AAATGCTCGC CAATCAAACC 
AGGAATGATT TCCGAAGCGG TTGCACATTC CGAGTCAACG GTGATCTTGG TCAGCCTCAA 
CCTGTATATA TCCACAGAGG AAATTATTTA AGTCCAACTG GTAATACAGG TCAGATTCGT 
CTTAATCGTG GGGAACAAGT TCTTATCGCT TGCACAGGAT CCGGCCGTAC GATCCGTCAC 
CCCAATGTTG CTTCTAACCT TGCTGTCGGG ACGGTCAGCT GTCAAAACAA TAATCTCGTC 
ACAGCCAATT GGCTGCGTGG TAACAGTGCT TTCGGGCAAC TCACATGCTC TTCTCACGCT 
TACCATGACG CTCAACAAAC CAACACTAGA TGCTTCAATA ATCACTTTGT TATTCGTGTG 
GGATTCATCG TCAACAATGT ATTTTATCCT CTATATTGGT CCTGCTTTGA CCGCAACCGC 
TTAGAGGTTC TATATGTATG GTACCACCAA AACCCACCTA ATTCCGTATT CCAGTCTCGC 
GTTGATAGGC CTAGTTGGAT AGCTGGAAAC TTCTTCCCCG GTGTAGCTGT TAACTCTGCC 
TATACTCAAG TATCTCAAAG AAACATGATC GCTGGATTCG TGGGTAATGC CCTCGCAGAC 
AGATATGTAA CGTCCACGCA GTTCTTAGCA CGGGGTCATC TTGCTGCTAA GACTGACTTT 
ATATATGCAA CCGGTCAACG TGCTAGCTTC TACTTTATTA ACGCTGCTCC TCAATGGCAG 
CCTTTCAACG CTGGAAACTG GAACCGGCTC GAGCAGAATC TTCGTCGTAG AATTGGACAG 
GCTGGATATC ATACCATGGT CTACACTGGA ACATTCCGCG TAACACAACT TAGAAATCAA 
AACAACCGAC TTGTTGATAT TTTCTTGCAT CGTGCATCCA ACGGCGCTCT TCAAATTCCT 
GTACCACTTT ATTTCTACAA GGTGGTGCAG GACTCTTCTC GTCGCTTCGG TACCGCCTTT 
ATCAGTATCA ACAATCCTTA CTACACCCAA GCGGAGGCTC GCAATCTGCA GTTCTGCACA 
GACCGTTGCC GCAATAACAA CGCATTCAAC TGGGTGGGGT GGCAACCTGA TCGCATCGAC 
CTCGGCTATA GCTTCTGCTG TACCATTGCT GATTTCCGAA GAACTATTCC GCACCTTCCA 
GCTTTCAATG TTAACGGACT TCTGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUC ( XM_012691673.2 ) cDNA ORF clone, Bombyx mori(Domestic silkworm) -> XP_012547127.1 Bombyx mori alkaline nuclease (Nuc), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBg14183
Clone ID Related Accession (Same CDS sequence)	XM_012691673.2 , XM_012691673.1
Accession Version	XM_012691673.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1496505600000
Organism	Bombyx mori(domestic silkworm)
Product	alkaline nuclease isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004582008.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 5, 2017 this sequence version replaced XM_012691673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCGTCTGA CGCTTGTTCT CGCAGCATTA GCTGTAGCGG TGGTGGCACT GCCCTCCAAA 
CTTCGAACGG ATCTACCAGA GCCAGGACAA CTGGCTTTTG TTCTTAACGA AGATGAGTTT 
GAAGACTACC TCGATGCCTA TCTCGCTCTT CAACAATCTG AAATGCTCGC CAATCAAACC 
AGGAATGATT TCCGAAGCGG TTGCACATTC CGAGTCAACG GTGATCTTGG TCAGCCTCAA 
CCTGTATATA TCCACAGAGG AAATTATTTA AGTCCAACTG GTAATACAGG TCAGATTCGT 
CTTAATCGTG GGGAACAAGT TCTTATCGCT TGCACAGGAT CCGGCCGTAC GATCCGTCAC 
CCCAATGTTG CTTCTAACCT TGCTGTCGGG ACGGTCAGCT GTCAAAACAA TAATCTCGTC 
ACAGCCAATT GGCTGCGTGG TAACAGTGCT TTCGGGCAAC TCACATGCTC TTCTCACGCT 
CACCATGACG CTCAACAAAC CAACACTAGA TGCTTCAATA ATCACTTTGT TATTCGTGTG 
GGATTCATCG TCAACAATGT ATTTTATCCT CTATATTGGT CCTGCTTTGA CCGCAACCGC 
TTAGAGGTTC TATATGTATG GTACCACCAA AACCCACCTA ATTCCGTATT CCAGTCTCGC 
GTTGATAGGC CTAGTTGGAT AGCTGGAAAC TTCTTCCCCG GTGTAGCTGT TAACTCTGCC 
TATACTCAAG TATCTCAAAG AAACATGATC GCTGGATTCG TGGGTAATGC CCTCGCAGAC 
AGATATGTAA CGTCCACGCA GTTCTTAGCA CGGGGTCATC TTGCTGCTAA GACTGACTTT 
ATATATGCAA CCGGTCAACG TGCTAGCTTC TACTTTATTA ACGCTGCTCC TCAATGGCAG 
CCTTTCAACG CTGGAAACTG GAACCGGCTC GAGCAGAATC TTCGTCGTAG AATTGGACAG 
GCTGGATATC ATACCATGGT CTACACTGGA ACATTCCGCG TAACACAACT TAGAAATCAA 
AACAACCGAC TTGTTGATAT TTTCTTGCAT CGTGCATCCA ACGGCGCTCT TCAAATTCCT 
GTACCACTTT ATTTCTACAA GGTGGTGCAT GACTCCTCTC GTCGCCTCGG TACCGCCTTT 
ATCAGTATCA ACAATCCTTA CTACACCCAA GCGGAGGCTC GCAATCTGCA GTTCTGCACA 
GACCGTTGCC GCAATAACAA CGCATTCAAC TGGGTGGGGT GGCAACCTGA TCGCATCGAC 
CTCGGCTATA GCTTCTGCTG TACCATTGCT GATTTCCGAA GAACTATTCC GCACCTTCCA 
GCTTTCAATG TTAACGGACT TCTGACAATA AAAATGGCGC TATTTATTTT TCTGATAATC 
ACCTATACAG TGACAGTTAT TTCCTGGCAC ATAGAACTTC CGGAGCCAAG CCGTTTCGCA 
TTTGATCTCA ATGAAGACGA TTTTGAAGAC TATCTGGACT CGTGGTTAGA AATAGAAGAA 
AGAAAGTGGC AAATTAATGA CAGGGCTACT CCTGCAACAG GCTGCGCATT TAAAGTCAAT 
GGGGATCTAG GCGAACCCCA GCCGGTGTAT CTACGAGGTA GCAATTACAT AATTCCAACA 
GGAACAACAG GTCAGATCCA TCTGAGTGTT GGAGAACAAA TATTTGTCAC ATGTACAGGG 
ACTAAAAGAG TTATTCAACA TCCTAACGTT ACTTCGTCAA CTAGCTCTGG TCTCGCCACT 
TGCGTCAGCA ATACGCTAGT TTCTGGTAAC GACTGGTTCC ATGGCCACAG TGAATTTAGA 
AACATCACAT GCTCAGGTCA TTCACGGTAT GAAGCTCAGG CTACATCAGA AACATGCTAT 
GGAAATAATA CTGTTATCAG GGTCGGTTTC GTTATAAACA ATTTTTTCTA TCCATTGTAC 
TGGTCATGCT TTGACCAGAA CCGCCTGGAG GTTTTATATG CGTGGTACGA GCAGAATCCC 
CCCAATGCAG TTCACCAAAC AGGAGTTACC AGACCTAGCT GGTTAGCTGG AGGTTTTTTC 
CCTGGGGTCT CCGTCAATAC AATGTACACG CAAGCTCAAC AAAAAACTAT ACTAGCGTCC 
CTCGTGGGTA CAGAACTTGC TGGCAAATAC ATTACAAATT CACAACATAT GGCCCGTGGC 
CACCTCGCCG CGAAAACTGA CTTCATTTTT GCTACTGGTC AGCGAGCCAC GTTTTTTTTC 
ATCAATGCCG CCCCTCAGTG GCAACCATTT AATGCGGGTA ACTGGAACTC GCTTGAACAG 
AAACTCCGTG CACGCATTGG TGAAGCAGGA TATAATACTA CTGTTTACAC GGGAACCTTC 
GGCGTAACCC AACTACGGGA CCAAAATGGC CAACTTGTTG ATATTTACCT TTACCGGGAC 
ACTAACAATA ACCCTCAGAT ACCTGTTCCG TTGTACTTTT ACAAAGTTGT GTATGAGCCT 
GTCCTACAAA AGGGCACAGC TTTTGTGGCG ATAAACAACC CGTACTACAC TGAAACTGAA 
GTACGCGCCT TGCAATTCTG CACTGACCAC TGCCGCAACA ACACCGCGTT CAACTGGATT 
GGCTGGCAAC CAGATCGAAT CGACATCGGG TACAGCTTCT GCTGTACCAT CGAAGACTTT 
AGACGGGCTG TGCCGCATTT GCCAGACTTT AATGTTACCG GGCTTTTGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012547127.1
CDS	123..2816
Translation	MRLTLVLAALAVAVVALPSKLRTDLPEPGQLAFVLNEDEFEDYLDAYLALQQSEMLANQTRNDFRSGCTFRVNGDLGQPQPVYIHRGNYLSPTGNTGQIRLNRGEQVLIACTGSGRTIRHPNVASNLAVGTVSCQNNNLVTANWLRGNSAFGQLTCSSHAHHDAQQTNTRCFNNHFVIRVGFIVNNVFYPLYWSCFDRNRLEVLYVWYHQNPPNSVFQSRVDRPSWIAGNFFPGVAVNSAYTQVSQRNMIAGFVGNALADRYVTSTQFLARGHLAAKTDFIYATGQRASFYFINAAPQWQPFNAGNWNRLEQNLRRRIGQAGYHTMVYTGTFRVTQLRNQNNRLVDIFLHRASNGALQIPVPLYFYKVVHDSSRRLGTAFISINNPYYTQAEARNLQFCTDRCRNNNAFNWVGWQPDRIDLGYSFCCTIADFRRTIPHLPAFNVNGLLTIKMALFIFLIITYTVTVISWHIELPEPSRFAFDLNEDDFEDYLDSWLEIEERKWQINDRATPATGCAFKVNGDLGEPQPVYLRGSNYIIPTGTTGQIHLSVGEQIFVTCTGTKRVIQHPNVTSSTSSGLATCVSNTLVSGNDWFHGHSEFRNITCSGHSRYEAQATSETCYGNNTVIRVGFVINNFFYPLYWSCFDQNRLEVLYAWYEQNPPNAVHQTGVTRPSWLAGGFFPGVSVNTMYTQAQQKTILASLVGTELAGKYITNSQHMARGHLAAKTDFIFATGQRATFFFINAAPQWQPFNAGNWNSLEQKLRARIGEAGYNTTVYTGTFGVTQLRDQNGQLVDIYLYRDTNNNPQIPVPLYFYKVVYEPVLQKGTAFVAINNPYYTETEVRALQFCTDHCRNNTAFNWIGWQPDRIDIGYSFCCTIEDFRRAVPHLPDFNVTGLLY

Target ORF information:

RefSeq Version	XM_012691673.2
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori alkaline nuclease (Nuc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012691673.2

ORF Insert Sequence:

ATGCGTCTGA CGCTTGTTCT CGCAGCATTA GCTGTAGCGG TGGTGGCACT GCCCTCCAAA 
CTTCGAACGG ATCTACCAGA GCCAGGACAA CTGGCTTTTG TTCTTAACGA AGATGAGTTT 
GAAGACTACC TCGATGCCTA TCTCGCTCTT CAACAATCTG AAATGCTCGC CAATCAAACC 
AGGAATGATT TCCGAAGCGG TTGCACATTC CGAGTCAACG GTGATCTTGG TCAGCCTCAA 
CCTGTATATA TCCACAGAGG AAATTATTTA AGTCCAACTG GTAATACAGG TCAGATTCGT 
CTTAATCGTG GGGAACAAGT TCTTATCGCT TGCACAGGAT CCGGCCGTAC GATCCGTCAC 
CCCAATGTTG CTTCTAACCT TGCTGTCGGG ACGGTCAGCT GTCAAAACAA TAATCTCGTC 
ACAGCCAATT GGCTGCGTGG TAACAGTGCT TTCGGGCAAC TCACATGCTC TTCTCACGCT 
CACCATGACG CTCAACAAAC CAACACTAGA TGCTTCAATA ATCACTTTGT TATTCGTGTG 
GGATTCATCG TCAACAATGT ATTTTATCCT CTATATTGGT CCTGCTTTGA CCGCAACCGC 
TTAGAGGTTC TATATGTATG GTACCACCAA AACCCACCTA ATTCCGTATT CCAGTCTCGC 
GTTGATAGGC CTAGTTGGAT AGCTGGAAAC TTCTTCCCCG GTGTAGCTGT TAACTCTGCC 
TATACTCAAG TATCTCAAAG AAACATGATC GCTGGATTCG TGGGTAATGC CCTCGCAGAC 
AGATATGTAA CGTCCACGCA GTTCTTAGCA CGGGGTCATC TTGCTGCTAA GACTGACTTT 
ATATATGCAA CCGGTCAACG TGCTAGCTTC TACTTTATTA ACGCTGCTCC TCAATGGCAG 
CCTTTCAACG CTGGAAACTG GAACCGGCTC GAGCAGAATC TTCGTCGTAG AATTGGACAG 
GCTGGATATC ATACCATGGT CTACACTGGA ACATTCCGCG TAACACAACT TAGAAATCAA 
AACAACCGAC TTGTTGATAT TTTCTTGCAT CGTGCATCCA ACGGCGCTCT TCAAATTCCT 
GTACCACTTT ATTTCTACAA GGTGGTGCAT GACTCCTCTC GTCGCCTCGG TACCGCCTTT 
ATCAGTATCA ACAATCCTTA CTACACCCAA GCGGAGGCTC GCAATCTGCA GTTCTGCACA 
GACCGTTGCC GCAATAACAA CGCATTCAAC TGGGTGGGGT GGCAACCTGA TCGCATCGAC 
CTCGGCTATA GCTTCTGCTG TACCATTGCT GATTTCCGAA GAACTATTCC GCACCTTCCA 
GCTTTCAATG TTAACGGACT TCTGACAATA AAAATGGCGC TATTTATTTT TCTGATAATC 
ACCTATACAG TGACAGTTAT TTCCTGGCAC ATAGAACTTC CGGAGCCAAG CCGTTTCGCA 
TTTGATCTCA ATGAAGACGA TTTTGAAGAC TATCTGGACT CGTGGTTAGA AATAGAAGAA 
AGAAAGTGGC AAATTAATGA CAGGGCTACT CCTGCAACAG GCTGCGCATT TAAAGTCAAT 
GGGGATCTAG GCGAACCCCA GCCGGTGTAT CTACGAGGTA GCAATTACAT AATTCCAACA 
GGAACAACAG GTCAGATCCA TCTGAGTGTT GGAGAACAAA TATTTGTCAC ATGTACAGGG 
ACTAAAAGAG TTATTCAACA TCCTAACGTT ACTTCGTCAA CTAGCTCTGG TCTCGCCACT 
TGCGTCAGCA ATACGCTAGT TTCTGGTAAC GACTGGTTCC ATGGCCACAG TGAATTTAGA 
AACATCACAT GCTCAGGTCA TTCACGGTAT GAAGCTCAGG CTACATCAGA AACATGCTAT 
GGAAATAATA CTGTTATCAG GGTCGGTTTC GTTATAAACA ATTTTTTCTA TCCATTGTAC 
TGGTCATGCT TTGACCAGAA CCGCCTGGAG GTTTTATATG CGTGGTACGA GCAGAATCCC 
CCCAATGCAG TTCACCAAAC AGGAGTTACC AGACCTAGCT GGTTAGCTGG AGGTTTTTTC 
CCTGGGGTCT CCGTCAATAC AATGTACACG CAAGCTCAAC AAAAAACTAT ACTAGCGTCC 
CTCGTGGGTA CAGAACTTGC TGGCAAATAC ATTACAAATT CACAACATAT GGCCCGTGGC 
CACCTCGCCG CGAAAACTGA CTTCATTTTT GCTACTGGTC AGCGAGCCAC GTTTTTTTTC 
ATCAATGCCG CCCCTCAGTG GCAACCATTT AATGCGGGTA ACTGGAACTC GCTTGAACAG 
AAACTCCGTG CACGCATTGG TGAAGCAGGA TATAATACTA CTGTTTACAC GGGAACCTTC 
GGCGTAACCC AACTACGGGA CCAAAATGGC CAACTTGTTG ATATTTACCT TTACCGGGAC 
ACTAACAATA ACCCTCAGAT ACCTGTTCCG TTGTACTTTT ACAAAGTTGT GTATGAGCCT 
GTCCTACAAA AGGGCACAGC TTTTGTGGCG ATAAACAACC CGTACTACAC TGAAACTGAA 
GTACGCGCCT TGCAATTCTG CACTGACCAC TGCCGCAACA ACACCGCGTT CAACTGGATT 
GGCTGGCAAC CAGATCGAAT CGACATCGGG TACAGCTTCT GCTGTACCAT CGAAGACTTT 
AGACGGGCTG TGCCGCATTT GCCAGACTTT AATGTTACCG GGCTTTTGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUC ( XM_012691673.1 ) cDNA ORF clone, Bombyx mori(Domestic silkworm) -> XP_012547127.1 Bombyx mori alkaline nuclease (Nuc), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBg14183
Clone ID Related Accession (Same CDS sequence)	XM_012691673.2 , XM_012691673.1
Accession Version	XM_012691673.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433347200000
Organism	Bombyx mori(Domestic silkworm)
Product	alkaline nuclease isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004582008.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCGTCTGA CGCTTGTTCT CGCAGCATTA GCTGTAGCGG TGGTGGCACT GCCCTCCAAA 
CTTCGAACGG ATCTACCAGA GCCAGGACAA CTGGCTTTTG TTCTTAACGA AGATGAGTTT 
GAAGACTACC TCGATGCCTA TCTCGCTCTT CAACAATCTG AAATGCTCGC CAATCAAACC 
AGGAATGATT TCCGAAGCGG TTGCACATTC CGAGTCAACG GTGATCTTGG TCAGCCTCAA 
CCTGTATATA TCCACAGAGG AAATTATTTA AGTCCAACTG GTAATACAGG TCAGATTCGT 
CTTAATCGTG GGGAACAAGT TCTTATCGCT TGCACAGGAT CCGGCCGTAC GATCCGTCAC 
CCCAATGTTG CTTCTAACCT TGCTGTCGGG ACGGTCAGCT GTCAAAACAA TAATCTCGTC 
ACAGCCAATT GGCTGCGTGG TAACAGTGCT TTCGGGCAAC TCACATGCTC TTCTCACGCT 
CACCATGACG CTCAACAAAC CAACACTAGA TGCTTCAATA ATCACTTTGT TATTCGTGTG 
GGATTCATCG TCAACAATGT ATTTTATCCT CTATATTGGT CCTGCTTTGA CCGCAACCGC 
TTAGAGGTTC TATATGTATG GTACCACCAA AACCCACCTA ATTCCGTATT CCAGTCTCGC 
GTTGATAGGC CTAGTTGGAT AGCTGGAAAC TTCTTCCCCG GTGTAGCTGT TAACTCTGCC 
TATACTCAAG TATCTCAAAG AAACATGATC GCTGGATTCG TGGGTAATGC CCTCGCAGAC 
AGATATGTAA CGTCCACGCA GTTCTTAGCA CGGGGTCATC TTGCTGCTAA GACTGACTTT 
ATATATGCAA CCGGTCAACG TGCTAGCTTC TACTTTATTA ACGCTGCTCC TCAATGGCAG 
CCTTTCAACG CTGGAAACTG GAACCGGCTC GAGCAGAATC TTCGTCGTAG AATTGGACAG 
GCTGGATATC ATACCATGGT CTACACTGGA ACATTCCGCG TAACACAACT TAGAAATCAA 
AACAACCGAC TTGTTGATAT TTTCTTGCAT CGTGCATCCA ACGGCGCTCT TCAAATTCCT 
GTACCACTTT ATTTCTACAA GGTGGTGCAT GACTCCTCTC GTCGCCTCGG TACCGCCTTT 
ATCAGTATCA ACAATCCTTA CTACACCCAA GCGGAGGCTC GCAATCTGCA GTTCTGCACA 
GACCGTTGCC GCAATAACAA CGCATTCAAC TGGGTGGGGT GGCAACCTGA TCGCATCGAC 
CTCGGCTATA GCTTCTGCTG TACCATTGCT GATTTCCGAA GAACTATTCC GCACCTTCCA 
GCTTTCAATG TTAACGGACT TCTGACAATA AAAATGGCGC TATTTATTTT TCTGATAATC 
ACCTATACAG TGACAGTTAT TTCCTGGCAC ATAGAACTTC CGGAGCCAAG CCGTTTCGCA 
TTTGATCTCA ATGAAGACGA TTTTGAAGAC TATCTGGACT CGTGGTTAGA AATAGAAGAA 
AGAAAGTGGC AAATTAATGA CAGGGCTACT CCTGCAACAG GCTGCGCATT TAAAGTCAAT 
GGGGATCTAG GCGAACCCCA GCCGGTGTAT CTACGAGGTA GCAATTACAT AATTCCAACA 
GGAACAACAG GTCAGATCCA TCTGAGTGTT GGAGAACAAA TATTTGTCAC ATGTACAGGG 
ACTAAAAGAG TTATTCAACA TCCTAACGTT ACTTCGTCAA CTAGCTCTGG TCTCGCCACT 
TGCGTCAGCA ATACGCTAGT TTCTGGTAAC GACTGGTTCC ATGGCCACAG TGAATTTAGA 
AACATCACAT GCTCAGGTCA TTCACGGTAT GAAGCTCAGG CTACATCAGA AACATGCTAT 
GGAAATAATA CTGTTATCAG GGTCGGTTTC GTTATAAACA ATTTTTTCTA TCCATTGTAC 
TGGTCATGCT TTGACCAGAA CCGCCTGGAG GTTTTATATG CGTGGTACGA GCAGAATCCC 
CCCAATGCAG TTCACCAAAC AGGAGTTACC AGACCTAGCT GGTTAGCTGG AGGTTTTTTC 
CCTGGGGTCT CCGTCAATAC AATGTACACG CAAGCTCAAC AAAAAACTAT ACTAGCGTCC 
CTCGTGGGTA CAGAACTTGC TGGCAAATAC ATTACAAATT CACAACATAT GGCCCGTGGC 
CACCTCGCCG CGAAAACTGA CTTCATTTTT GCTACTGGTC AGCGAGCCAC GTTTTTTTTC 
ATCAATGCCG CCCCTCAGTG GCAACCATTT AATGCGGGTA ACTGGAACTC GCTTGAACAG 
AAACTCCGTG CACGCATTGG TGAAGCAGGA TATAATACTA CTGTTTACAC GGGAACCTTC 
GGCGTAACCC AACTACGGGA CCAAAATGGC CAACTTGTTG ATATTTACCT TTACCGGGAC 
ACTAACAATA ACCCTCAGAT ACCTGTTCCG TTGTACTTTT ACAAAGTTGT GTATGAGCCT 
GTCCTACAAA AGGGCACAGC TTTTGTGGCG ATAAACAACC CGTACTACAC TGAAACTGAA 
GTACGCGCCT TGCAATTCTG CACTGACCAC TGCCGCAACA ACACCGCGTT CAACTGGATT 
GGCTGGCAAC CAGATCGAAT CGACATCGGG TACAGCTTCT GCTGTACCAT CGAAGACTTT 
AGACGGGCTG TGCCGCATTT GCCAGACTTT AATGTTACCG GGCTTTTGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012547127.1
CDS	47..2740
Translation	MRLTLVLAALAVAVVALPSKLRTDLPEPGQLAFVLNEDEFEDYLDAYLALQQSEMLANQTRNDFRSGCTFRVNGDLGQPQPVYIHRGNYLSPTGNTGQIRLNRGEQVLIACTGSGRTIRHPNVASNLAVGTVSCQNNNLVTANWLRGNSAFGQLTCSSHAHHDAQQTNTRCFNNHFVIRVGFIVNNVFYPLYWSCFDRNRLEVLYVWYHQNPPNSVFQSRVDRPSWIAGNFFPGVAVNSAYTQVSQRNMIAGFVGNALADRYVTSTQFLARGHLAAKTDFIYATGQRASFYFINAAPQWQPFNAGNWNRLEQNLRRRIGQAGYHTMVYTGTFRVTQLRNQNNRLVDIFLHRASNGALQIPVPLYFYKVVHDSSRRLGTAFISINNPYYTQAEARNLQFCTDRCRNNNAFNWVGWQPDRIDLGYSFCCTIADFRRTIPHLPAFNVNGLLTIKMALFIFLIITYTVTVISWHIELPEPSRFAFDLNEDDFEDYLDSWLEIEERKWQINDRATPATGCAFKVNGDLGEPQPVYLRGSNYIIPTGTTGQIHLSVGEQIFVTCTGTKRVIQHPNVTSSTSSGLATCVSNTLVSGNDWFHGHSEFRNITCSGHSRYEAQATSETCYGNNTVIRVGFVINNFFYPLYWSCFDQNRLEVLYAWYEQNPPNAVHQTGVTRPSWLAGGFFPGVSVNTMYTQAQQKTILASLVGTELAGKYITNSQHMARGHLAAKTDFIFATGQRATFFFINAAPQWQPFNAGNWNSLEQKLRARIGEAGYNTTVYTGTFGVTQLRDQNGQLVDIYLYRDTNNNPQIPVPLYFYKVVYEPVLQKGTAFVAINNPYYTETEVRALQFCTDHCRNNTAFNWIGWQPDRIDIGYSFCCTIEDFRRAVPHLPDFNVTGLLY

Target ORF information:

RefSeq Version	XM_012691673.1
Organism	Bombyx mori(Domestic silkworm)
Definition	Bombyx mori alkaline nuclease (Nuc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012691673.1

ORF Insert Sequence:

ATGCGTCTGA CGCTTGTTCT CGCAGCATTA GCTGTAGCGG TGGTGGCACT GCCCTCCAAA 
CTTCGAACGG ATCTACCAGA GCCAGGACAA CTGGCTTTTG TTCTTAACGA AGATGAGTTT 
GAAGACTACC TCGATGCCTA TCTCGCTCTT CAACAATCTG AAATGCTCGC CAATCAAACC 
AGGAATGATT TCCGAAGCGG TTGCACATTC CGAGTCAACG GTGATCTTGG TCAGCCTCAA 
CCTGTATATA TCCACAGAGG AAATTATTTA AGTCCAACTG GTAATACAGG TCAGATTCGT 
CTTAATCGTG GGGAACAAGT TCTTATCGCT TGCACAGGAT CCGGCCGTAC GATCCGTCAC 
CCCAATGTTG CTTCTAACCT TGCTGTCGGG ACGGTCAGCT GTCAAAACAA TAATCTCGTC 
ACAGCCAATT GGCTGCGTGG TAACAGTGCT TTCGGGCAAC TCACATGCTC TTCTCACGCT 
CACCATGACG CTCAACAAAC CAACACTAGA TGCTTCAATA ATCACTTTGT TATTCGTGTG 
GGATTCATCG TCAACAATGT ATTTTATCCT CTATATTGGT CCTGCTTTGA CCGCAACCGC 
TTAGAGGTTC TATATGTATG GTACCACCAA AACCCACCTA ATTCCGTATT CCAGTCTCGC 
GTTGATAGGC CTAGTTGGAT AGCTGGAAAC TTCTTCCCCG GTGTAGCTGT TAACTCTGCC 
TATACTCAAG TATCTCAAAG AAACATGATC GCTGGATTCG TGGGTAATGC CCTCGCAGAC 
AGATATGTAA CGTCCACGCA GTTCTTAGCA CGGGGTCATC TTGCTGCTAA GACTGACTTT 
ATATATGCAA CCGGTCAACG TGCTAGCTTC TACTTTATTA ACGCTGCTCC TCAATGGCAG 
CCTTTCAACG CTGGAAACTG GAACCGGCTC GAGCAGAATC TTCGTCGTAG AATTGGACAG 
GCTGGATATC ATACCATGGT CTACACTGGA ACATTCCGCG TAACACAACT TAGAAATCAA 
AACAACCGAC TTGTTGATAT TTTCTTGCAT CGTGCATCCA ACGGCGCTCT TCAAATTCCT 
GTACCACTTT ATTTCTACAA GGTGGTGCAT GACTCCTCTC GTCGCCTCGG TACCGCCTTT 
ATCAGTATCA ACAATCCTTA CTACACCCAA GCGGAGGCTC GCAATCTGCA GTTCTGCACA 
GACCGTTGCC GCAATAACAA CGCATTCAAC TGGGTGGGGT GGCAACCTGA TCGCATCGAC 
CTCGGCTATA GCTTCTGCTG TACCATTGCT GATTTCCGAA GAACTATTCC GCACCTTCCA 
GCTTTCAATG TTAACGGACT TCTGACAATA AAAATGGCGC TATTTATTTT TCTGATAATC 
ACCTATACAG TGACAGTTAT TTCCTGGCAC ATAGAACTTC CGGAGCCAAG CCGTTTCGCA 
TTTGATCTCA ATGAAGACGA TTTTGAAGAC TATCTGGACT CGTGGTTAGA AATAGAAGAA 
AGAAAGTGGC AAATTAATGA CAGGGCTACT CCTGCAACAG GCTGCGCATT TAAAGTCAAT 
GGGGATCTAG GCGAACCCCA GCCGGTGTAT CTACGAGGTA GCAATTACAT AATTCCAACA 
GGAACAACAG GTCAGATCCA TCTGAGTGTT GGAGAACAAA TATTTGTCAC ATGTACAGGG 
ACTAAAAGAG TTATTCAACA TCCTAACGTT ACTTCGTCAA CTAGCTCTGG TCTCGCCACT 
TGCGTCAGCA ATACGCTAGT TTCTGGTAAC GACTGGTTCC ATGGCCACAG TGAATTTAGA 
AACATCACAT GCTCAGGTCA TTCACGGTAT GAAGCTCAGG CTACATCAGA AACATGCTAT 
GGAAATAATA CTGTTATCAG GGTCGGTTTC GTTATAAACA ATTTTTTCTA TCCATTGTAC 
TGGTCATGCT TTGACCAGAA CCGCCTGGAG GTTTTATATG CGTGGTACGA GCAGAATCCC 
CCCAATGCAG TTCACCAAAC AGGAGTTACC AGACCTAGCT GGTTAGCTGG AGGTTTTTTC 
CCTGGGGTCT CCGTCAATAC AATGTACACG CAAGCTCAAC AAAAAACTAT ACTAGCGTCC 
CTCGTGGGTA CAGAACTTGC TGGCAAATAC ATTACAAATT CACAACATAT GGCCCGTGGC 
CACCTCGCCG CGAAAACTGA CTTCATTTTT GCTACTGGTC AGCGAGCCAC GTTTTTTTTC 
ATCAATGCCG CCCCTCAGTG GCAACCATTT AATGCGGGTA ACTGGAACTC GCTTGAACAG 
AAACTCCGTG CACGCATTGG TGAAGCAGGA TATAATACTA CTGTTTACAC GGGAACCTTC 
GGCGTAACCC AACTACGGGA CCAAAATGGC CAACTTGTTG ATATTTACCT TTACCGGGAC 
ACTAACAATA ACCCTCAGAT ACCTGTTCCG TTGTACTTTT ACAAAGTTGT GTATGAGCCT 
GTCCTACAAA AGGGCACAGC TTTTGTGGCG ATAAACAACC CGTACTACAC TGAAACTGAA 
GTACGCGCCT TGCAATTCTG CACTGACCAC TGCCGCAACA ACACCGCGTT CAACTGGATT 
GGCTGGCAAC CAGATCGAAT CGACATCGGG TACAGCTTCT GCTGTACCAT CGAAGACTTT 
AGACGGGCTG TGCCGCATTT GCCAGACTTT AATGTTACCG GGCTTTTGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
BMC genomics15820(2014 Sep)
Ou J,Deng HM,Zheng SC,Huang LH,Feng QL,Liu L

Large scale full-length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori.
G3 (Bethesda, Md.)3(9)1481-92(2013 Sep)
Suetsugu Y,Futahashi R,Kanamori H,Kadono-Okuda K,Sasanuma S,Narukawa J,Ajimura M,Jouraku A,Namiki N,Shimomura M,Sezutsu H,Osanai-Futahashi M,Suzuki MG,Daimon T,Shinoda T,Taniai K,Asaoka K,Niwa R,Kawaoka S,Katsuma S,Tamura T,Noda H,Kasahara M,Sugano S,Suzuki Y,Fujiwara H,Kataoka H,Arunkumar KP,Tomar A,Nagaraju J,Goldsmith MR,Feng Q,Xia Q,Yamamoto K,Shimada T,Mita K

Bombyx mori DNA/RNA non-specific nuclease: expression of isoforms in insect culture cells, subcellular localization and functional assays.
Journal of insect physiology58(8)1166-76(2012 Aug)
Liu J,Swevers L,Iatrou K,Huvenne H,Smagghe G

Molecular characterization of a cDNA encoding extracellular dsRNase and its expression in the silkworm, Bombyx mori.
Insect biochemistry and molecular biology37(2)176-83(2007 Feb)
Arimatsu Y,Kotani E,Sugimura Y,Furusawa T

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NUC cDNA ORF clone, Bombyx mori(Domestic silkworm)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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