CPR146 cDNA ORF clone, Bombyx mori(domestic silkworm)

Gene
Clones
gRNAs

Summary

Gene Symbol	CPR146
Entrez Gene ID	693056
Full Name	collagen
Synonyms	BmorCPR146
Gene Type	protein-coding
Organism	Bombyx mori(domestic silkworm)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021351760.1	XP_021207435.1	collagen isoform X1
XM_012695260.1	XP_012550714.1	collagen isoform X1
XM_021351763.1	XP_021207438.1	collagen isoform X4
NM_001171643.1	NP_001165114.1	collagen precursor
XM_021351762.1	XP_021207437.1	collagen isoform X3
XM_021351761.1	XP_021207436.1	collagen isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
Ou J, Deng HM, Zheng SC, Huang LH, Feng QL, Liu L
BMC genomics15820(2014 Sep)

Genome-wide identification of cuticular protein genes in the silkworm, Bombyx mori.
Futahashi R, Okamoto S, Kawasaki H, Zhong YS, Iwanaga M, Mita K, Fujiwara H
Insect biochemistry and molecular biology38(12)1138-46(2008 Dec)

Identification of a Bombyx collagenous protein with multiple short domains of Gly-Xaa-Yaa repeats: cDNA characterization and regulation of expression.
Chareyre P, Besson MT, Fourche J, Bosquet G
Insect biochemistry and molecular biology26(7)677-85(1996 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CPR146 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CPR146 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBg27466	XM_021351760.1 Latest version!	Bombyx mori collagen (CPR146), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBg27467	XM_021351761.1 Latest version!	Bombyx mori collagen (CPR146), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBg27468	XM_021351762.1 Latest version!	Bombyx mori collagen (CPR146), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBg27469	XM_021351763.1 Latest version!	Bombyx mori collagen (CPR146), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBg13562	NM_001171643.1 Latest version!	Bombyx mori collagen (CPR146), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBg14076	XM_012695260.1 Latest version!	Bombyx mori collagen (CPR146), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CPR146 ( XM_021351760.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_021207435.1 Bombyx mori collagen (CPR146), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBg27466
Clone ID Related Accession (Same CDS sequence)	XM_021351760.1
Accession Version	XM_021351760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1496505600000
Organism	Bombyx mori(domestic silkworm)
Product	collagen isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004582025.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCCG GAATAATGAT TAAATTTTGT ATCTTGGCGG TGCTAGCATT GTCACTGGTT 
AGCGCCGATA AGCCAAAAAC TGAGACAACT TCAACGACCG CGAAAAAGGC AACTGAAGTA 
CCCGCGAAGA AGACAGTCGA AGTACCTTTG AAGAAGGCAT CTGAAGTGCC CGTGAAGAAG 
GCATCAGAAG TGCCAACAAA GAAGCCCCAT GTAGAACCCG CGCGTGAAGG AAGGCAGTTG 
AGTCCTTACC CTGGACAACA AGACAACGAA GTGGTGGTCG ACATTCAGGA CGACGAAAAG 
AAGCAGTACT ACGAAACCAA CTATGACACA AGTGCGTATG GTTTCGGATA CGATGTCGGC 
CCAAACGGAC AGTTTCATCA CGAGAACCGC GGTCCCGATG GTGTCGTCTA CGGATGTTAT 
GGTTACGTCG ACACCGAGGG CTACCTCCGC GCCACCCACT ACGTTGCCGA CAGCCACGGC 
TACCGAGTCG TAGAACCAGA AAAACCTGTC GAAGTTTTCC CCGATGAGAA ATACGAATAT 
GACGAAAATT ACGAACCCAC GCCATCCGCG ACGGGTCAAA CCATTCCGTG GAATAAGTTA 
TTCTTCCCCC AGGGATGCGG TCGCACTCCT GGCGGAGTTC CACCCCAACC GTTCAAACCA 
AAACCTAAAC CTAAACCCCC TGTAGATAGC ACCGGACAAA CTAGCAACCC CAGTCCTGGC 
GTCGCATACC CTGGTCAAGG CCCTAATGGC CCTTACGGTC CTGGCGGCTC CGGCGGCTCC 
GGCGGCCCTG GTGGTCCCGG AGGACCAAAT GGACCTTACG GACCTGGTGG TCCCGGAGGA 
CCAAATGGAC CTTACGGACC CGGCGGTACT GGCGGACCTA ATGGTCCTTA TGGCCCAGGT 
GGTCAAGATG GCTCATACAG ACCAGACGGC TCCTACGGTC CAGACGGATC TTATCAACCG 
GAAGGTTCCT ACGGACCCGA CGGATCATGG CAAGGTGGTT ACTATCCTGG CACTCCTGGA 
TCGCCTGGAA CTCCTGGGTC GCCTGGCAGC CCTGGATCCC CTGGTAGCCC GGGTACTCCT 
GGTTCTCCAG GCACACCAGG AACACCTGGT TCAGGCGGTG GATACTATCC TGGACAAGAC 
GGAACAGGGG GACCGACTGG TCCTTCAGGC GGGGGAGGTG GCTACTATCC AGGTAGTCCC 
GGCAGTCCTG GAACCCCCGG CACTCCAGGC ACACCCGGAA CACCAGGTGG CCCTGGGGGT 
CCAGGTGGAC CAGGTGGACC AGGTGGTCCA GGAGGGCCAA ACGGTCCAAA TGGGCCTGGA 
GGATCTGACG GCGGTCAATA TTATCCTGCA CTGCCCGGTA GTCCCGGTAC ACCCGGCTCC 
CCAGGCACTC CTGGTGGACC TGGTGGACCT GGTGGTCCCG GAGGCCCTGG GGGACCAGGC 
GGCCCAGGCG GCCCGGGAGG TCCAGATCAA CCAAGCTACC CTGGACAGCC AGGACAGCCT 
GGTACACCAG GATATCCAGG ACAACCAGGA TACCCAGGAC AACCCGGTAC ACCAGGACAA 
CCAGGAACAC CAGGCCAGCC AGGCTATCCA GGACAAGGTG GACAACCTAT AAAGCCTGGT 
CAGCCAGGAT ACCCAGGTCA GCCAGGCCAG CCGGGATACC CAGGACAACC AGGACAGCCA 
GGAACACCAG GACAGCCAGG AACACCAGGA CAGCCAGGAA CACCAGGCCA GCCAGGAACA 
CCAGGACAAC CAGGCCAGCC CGGAACACCA GGACAACCAG GTCAACCAGG CTACCCAGGA 
CAAGGTGGAC AGCCTATAAA GCCTGGTCAG CCAGGATACC CAGGTCAGCC AGGCCAGCCG 
GGATACCCTG GACAACCAGG ACAGCCAGGA GCACCAGGCC AACCGGGTCA GCCAGGACAA 
CCAGTGCAGC CCGGAACACC AGGACAAGCA GGTCAACCAG GCTACCCAGG ACAAGGTGGA 
CAACCTATAA AGCCTGCTCA GCCGGGACAC CCAGGCCAGC CTGGACAACC AGGGCAGCCC 
GGAACACCAG GACAACCAGG TCAACCAGGA TACCCAGGGC AACCGGGTCA GCCAGGACAA 
CCAGGGCAAC CAGGACAACA AGGCTATCCA GGACAAGGTG GACCACAACA GGGCGGTCAG 
CCTATTAATC CTAGTCAACC TGGTTACCCA GGCCAGTCAG GACAACCAGG TTACCCTGGA 
CAACCGGTTC AGCCAGGACA ACCAGGGCAG CCCGGAACAC CAGGGGAACC GGGACAGCCC 
GGTACACCAG GACAACCTGG CTATCCAGGA CAAGGTGGAC CACAACAGGG CGGTCAGCCT 
ATGAATCCTA GTCAACCCGA ATACCCAAGC CAGCCAGGAC AACCAGGACA GCCAGGAACA 
CCGGGACAAG CAGGACACCC AGGACAACCT GGACACCCAG GCGCACCAGG ACAACCAGGT 
TACCCAGGAC AACCCGGACA GACGGGCGCA CCAGGACAAG CAGGTTACCC AGGACAAGGT 
GGACCGCAAC AGGGCGGTCA GCCTATAAAT CCTAGTCAAC CAGGACATCC AGTGCAGCCA 
GGACAGCCGG GTCAACCAGG GCAGCCAGGA AAACCCGGGC AACCTGGTAC ACCAGGGCAG 
CCTGGAGCTC CAGGGCAACC TGGAACACCA GGACAACCAG GCTATCCAGG ACAAAATGGA 
CCACAACAAG GCGGTCAGCC TATTAAACCT GATCAACCCG GATACCCAGG CCAACCGGGA 
GCACCAGGAC AGCCAGGAAC ACCAGGACAA CCCGGATATC CAGGACAAGG CGGACCACAA 
CAGGGCGGTC AGCCTATCAA TCCCGATCAG CCCGGACAGC CAGGTCCACC AGGATATCCA 
GGGCAAGGCG GACAACCCAT AAATCCTAGT CAATCAGGCT ACCCGGGACA ACCAGGACAA 
GGAGGCGATT CCGTCTCGTC GGGAACTGGT GTGCAGCCAC CGACATATGT TCCATCAACT 
CAAATGCCAC CTTTCCCAAT TTATGTCATA CCTTTTCCAT TGCCTATCGT ACCAAGTCCA 
GGATCATGTC CCTGCTATTT ATTAAATCCT GGAAATAACG GTACAGCAAC GAACGAAATG 
CAAGCATCCT CACATCCCGC AAATGGACAG AGTCAAGGAT ATGCTCCGTA CGGTATTATA 
GGATTCATAC CTGTTGTTTT CGTTCCCTAT TGTCCAGGAA ATGGGAGTGC GATGAACACT 
GCACAACAAA ACTTCCCTCA AGCAGTACCG GTGCAATACA ATTGTGCCCA ATGTCAGGCC 
AATCGAGATG TCTACAGATT CGCAGGCCGA AACAATGGCG CCAGAGCGTT CAAAGAGCTT 
AAAGAACTTA AATCGCTGGA CGAACTTAAC GATCTCATTC GAAATAGAAT AAAACCTACC 
ACGAGGACAT TACGTCAAAT TGCAGCACAT CCAAAAATAC TTGAAACAAA ACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021207435.1
CDS	176..3652
Translation	MGPGIMIKFCILAVLALSLVSADKPKTETTSTTAKKATEVPAKKTVEVPLKKASEVPVKKASEVPTKKPHVEPAREGRQLSPYPGQQDNEVVVDIQDDEKKQYYETNYDTSAYGFGYDVGPNGQFHHENRGPDGVVYGCYGYVDTEGYLRATHYVADSHGYRVVEPEKPVEVFPDEKYEYDENYEPTPSATGQTIPWNKLFFPQGCGRTPGGVPPQPFKPKPKPKPPVDSTGQTSNPSPGVAYPGQGPNGPYGPGGSGGSGGPGGPGGPNGPYGPGGPGGPNGPYGPGGTGGPNGPYGPGGQDGSYRPDGSYGPDGSYQPEGSYGPDGSWQGGYYPGTPGSPGTPGSPGSPGSPGSPGTPGSPGTPGTPGSGGGYYPGQDGTGGPTGPSGGGGGYYPGSPGSPGTPGTPGTPGTPGGPGGPGGPGGPGGPGGPNGPNGPGGSDGGQYYPALPGSPGTPGSPGTPGGPGGPGGPGGPGGPGGPGGPGGPDQPSYPGQPGQPGTPGYPGQPGYPGQPGTPGQPGTPGQPGYPGQGGQPIKPGQPGYPGQPGQPGYPGQPGQPGTPGQPGTPGQPGTPGQPGTPGQPGQPGTPGQPGQPGYPGQGGQPIKPGQPGYPGQPGQPGYPGQPGQPGAPGQPGQPGQPVQPGTPGQAGQPGYPGQGGQPIKPAQPGHPGQPGQPGQPGTPGQPGQPGYPGQPGQPGQPGQPGQQGYPGQGGPQQGGQPINPSQPGYPGQSGQPGYPGQPVQPGQPGQPGTPGEPGQPGTPGQPGYPGQGGPQQGGQPMNPSQPEYPSQPGQPGQPGTPGQAGHPGQPGHPGAPGQPGYPGQPGQTGAPGQAGYPGQGGPQQGGQPINPSQPGHPVQPGQPGQPGQPGKPGQPGTPGQPGAPGQPGTPGQPGYPGQNGPQQGGQPIKPDQPGYPGQPGAPGQPGTPGQPGYPGQGGPQQGGQPINPDQPGQPGPPGYPGQGGQPINPSQSGYPGQPGQGGDSVSSGTGVQPPTYVPSTQMPPFPIYVIPFPLPIVPSPGSCPCYLLNPGNNGTATNEMQASSHPANGQSQGYAPYGIIGFIPVVFVPYCPGNGSAMNTAQQNFPQAVPVQYNCAQCQANRDVYRFAGRNNGARAFKELKELKSLDELNDLIRNRIKPTTRTLRQIAAHPKILETKH

Target ORF information:

RefSeq Version	XM_021351760.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori collagen (CPR146), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021351760.1

ORF Insert Sequence:

ATGGGACCCG GAATAATGAT TAAATTTTGT ATCTTGGCGG TGCTAGCATT GTCACTGGTT 
AGCGCCGATA AGCCAAAAAC TGAGACAACT TCAACGACCG CGAAAAAGGC AACTGAAGTA 
CCCGCGAAGA AGACAGTCGA AGTACCTTTG AAGAAGGCAT CTGAAGTGCC CGTGAAGAAG 
GCATCAGAAG TGCCAACAAA GAAGCCCCAT GTAGAACCCG CGCGTGAAGG AAGGCAGTTG 
AGTCCTTACC CTGGACAACA AGACAACGAA GTGGTGGTCG ACATTCAGGA CGACGAAAAG 
AAGCAGTACT ACGAAACCAA CTATGACACA AGTGCGTATG GTTTCGGATA CGATGTCGGC 
CCAAACGGAC AGTTTCATCA CGAGAACCGC GGTCCCGATG GTGTCGTCTA CGGATGTTAT 
GGTTACGTCG ACACCGAGGG CTACCTCCGC GCCACCCACT ACGTTGCCGA CAGCCACGGC 
TACCGAGTCG TAGAACCAGA AAAACCTGTC GAAGTTTTCC CCGATGAGAA ATACGAATAT 
GACGAAAATT ACGAACCCAC GCCATCCGCG ACGGGTCAAA CCATTCCGTG GAATAAGTTA 
TTCTTCCCCC AGGGATGCGG TCGCACTCCT GGCGGAGTTC CACCCCAACC GTTCAAACCA 
AAACCTAAAC CTAAACCCCC TGTAGATAGC ACCGGACAAA CTAGCAACCC CAGTCCTGGC 
GTCGCATACC CTGGTCAAGG CCCTAATGGC CCTTACGGTC CTGGCGGCTC CGGCGGCTCC 
GGCGGCCCTG GTGGTCCCGG AGGACCAAAT GGACCTTACG GACCTGGTGG TCCCGGAGGA 
CCAAATGGAC CTTACGGACC CGGCGGTACT GGCGGACCTA ATGGTCCTTA TGGCCCAGGT 
GGTCAAGATG GCTCATACAG ACCAGACGGC TCCTACGGTC CAGACGGATC TTATCAACCG 
GAAGGTTCCT ACGGACCCGA CGGATCATGG CAAGGTGGTT ACTATCCTGG CACTCCTGGA 
TCGCCTGGAA CTCCTGGGTC GCCTGGCAGC CCTGGATCCC CTGGTAGCCC GGGTACTCCT 
GGTTCTCCAG GCACACCAGG AACACCTGGT TCAGGCGGTG GATACTATCC TGGACAAGAC 
GGAACAGGGG GACCGACTGG TCCTTCAGGC GGGGGAGGTG GCTACTATCC AGGTAGTCCC 
GGCAGTCCTG GAACCCCCGG CACTCCAGGC ACACCCGGAA CACCAGGTGG CCCTGGGGGT 
CCAGGTGGAC CAGGTGGACC AGGTGGTCCA GGAGGGCCAA ACGGTCCAAA TGGGCCTGGA 
GGATCTGACG GCGGTCAATA TTATCCTGCA CTGCCCGGTA GTCCCGGTAC ACCCGGCTCC 
CCAGGCACTC CTGGTGGACC TGGTGGACCT GGTGGTCCCG GAGGCCCTGG GGGACCAGGC 
GGCCCAGGCG GCCCGGGAGG TCCAGATCAA CCAAGCTACC CTGGACAGCC AGGACAGCCT 
GGTACACCAG GATATCCAGG ACAACCAGGA TACCCAGGAC AACCCGGTAC ACCAGGACAA 
CCAGGAACAC CAGGCCAGCC AGGCTATCCA GGACAAGGTG GACAACCTAT AAAGCCTGGT 
CAGCCAGGAT ACCCAGGTCA GCCAGGCCAG CCGGGATACC CAGGACAACC AGGACAGCCA 
GGAACACCAG GACAGCCAGG AACACCAGGA CAGCCAGGAA CACCAGGCCA GCCAGGAACA 
CCAGGACAAC CAGGCCAGCC CGGAACACCA GGACAACCAG GTCAACCAGG CTACCCAGGA 
CAAGGTGGAC AGCCTATAAA GCCTGGTCAG CCAGGATACC CAGGTCAGCC AGGCCAGCCG 
GGATACCCTG GACAACCAGG ACAGCCAGGA GCACCAGGCC AACCGGGTCA GCCAGGACAA 
CCAGTGCAGC CCGGAACACC AGGACAAGCA GGTCAACCAG GCTACCCAGG ACAAGGTGGA 
CAACCTATAA AGCCTGCTCA GCCGGGACAC CCAGGCCAGC CTGGACAACC AGGGCAGCCC 
GGAACACCAG GACAACCAGG TCAACCAGGA TACCCAGGGC AACCGGGTCA GCCAGGACAA 
CCAGGGCAAC CAGGACAACA AGGCTATCCA GGACAAGGTG GACCACAACA GGGCGGTCAG 
CCTATTAATC CTAGTCAACC TGGTTACCCA GGCCAGTCAG GACAACCAGG TTACCCTGGA 
CAACCGGTTC AGCCAGGACA ACCAGGGCAG CCCGGAACAC CAGGGGAACC GGGACAGCCC 
GGTACACCAG GACAACCTGG CTATCCAGGA CAAGGTGGAC CACAACAGGG CGGTCAGCCT 
ATGAATCCTA GTCAACCCGA ATACCCAAGC CAGCCAGGAC AACCAGGACA GCCAGGAACA 
CCGGGACAAG CAGGACACCC AGGACAACCT GGACACCCAG GCGCACCAGG ACAACCAGGT 
TACCCAGGAC AACCCGGACA GACGGGCGCA CCAGGACAAG CAGGTTACCC AGGACAAGGT 
GGACCGCAAC AGGGCGGTCA GCCTATAAAT CCTAGTCAAC CAGGACATCC AGTGCAGCCA 
GGACAGCCGG GTCAACCAGG GCAGCCAGGA AAACCCGGGC AACCTGGTAC ACCAGGGCAG 
CCTGGAGCTC CAGGGCAACC TGGAACACCA GGACAACCAG GCTATCCAGG ACAAAATGGA 
CCACAACAAG GCGGTCAGCC TATTAAACCT GATCAACCCG GATACCCAGG CCAACCGGGA 
GCACCAGGAC AGCCAGGAAC ACCAGGACAA CCCGGATATC CAGGACAAGG CGGACCACAA 
CAGGGCGGTC AGCCTATCAA TCCCGATCAG CCCGGACAGC CAGGTCCACC AGGATATCCA 
GGGCAAGGCG GACAACCCAT AAATCCTAGT CAATCAGGCT ACCCGGGACA ACCAGGACAA 
GGAGGCGATT CCGTCTCGTC GGGAACTGGT GTGCAGCCAC CGACATATGT TCCATCAACT 
CAAATGCCAC CTTTCCCAAT TTATGTCATA CCTTTTCCAT TGCCTATCGT ACCAAGTCCA 
GGATCATGTC CCTGCTATTT ATTAAATCCT GGAAATAACG GTACAGCAAC GAACGAAATG 
CAAGCATCCT CACATCCCGC AAATGGACAG AGTCAAGGAT ATGCTCCGTA CGGTATTATA 
GGATTCATAC CTGTTGTTTT CGTTCCCTAT TGTCCAGGAA ATGGGAGTGC GATGAACACT 
GCACAACAAA ACTTCCCTCA AGCAGTACCG GTGCAATACA ATTGTGCCCA ATGTCAGGCC 
AATCGAGATG TCTACAGATT CGCAGGCCGA AACAATGGCG CCAGAGCGTT CAAAGAGCTT 
AAAGAACTTA AATCGCTGGA CGAACTTAAC GATCTCATTC GAAATAGAAT AAAACCTACC 
ACGAGGACAT TACGTCAAAT TGCAGCACAT CCAAAAATAC TTGAAACAAA ACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPR146 ( XM_021351761.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_021207436.1 Bombyx mori collagen (CPR146), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBg27467
Clone ID Related Accession (Same CDS sequence)	XM_021351761.1
Accession Version	XM_021351761.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1496505600000
Organism	Bombyx mori(domestic silkworm)
Product	collagen isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004582025.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCCG GAATAATGAT TAAATTTTGT ATCTTGGCGG TGCTAGCATT GTCACTGGTT 
AGCGCCGATA AGCCAAAAAC TGAGACAACT TCAACGACCG CGAAAAAGGC AACTGAAGTA 
CCCGCGAAGA AGACAGTCGA AGTACCTTTG AAGAAGGCAT CTGAAGTGCC CGTGAAGAAG 
GCATCAGAAG TGCCAACAAA GAAGCCCCAT GTAGAACCCG CGCGTGAAGG AAGGCAGTTG 
AGTCCTTACC CTGGACAACA AGACAACGAA GTGGTGGTCG ACATTCAGGA CGACGAAAAG 
AAGCAGTACT ACGAAACCAA CTATGACACA AGTGCGTATG GTTTCGGATA CGATGTCGGC 
CCAAACGGAC AGTTTCATCA CGAGAACCGC GGTCCCGATG GTGTCGTCTA CGGATGTTAT 
GGTTACGTCG ACACCGAGGG CTACCTCCGC GCCACCCACT ACGTTGCCGA CAGCCACGGC 
TACCGAGTCG TAGAACCAGA AAAACCTGTC GAAGTTTTCC CCGATGAGAA ATACGAATAT 
GACGAAAATT ACGAACCCAC GCCATCCGCG ACGGGTCAAA CCATTCCGTG GAATAAGTTA 
TTCTTCCCCC AGGGATGCGG TCGCACTCCT GGCGGAGTTC CACCCCAACC GTTCAAACCA 
AAACCTAAAC CTAAACCCCC TGTAGATAGC ACCGGACAAA CTAGCAACCC CAGTCCTGGC 
GTCGCATACC CTGGTCAAGG CCCTAATGGC CCTTACGGTC CTGGCGGCTC CGGCGGCTCC 
GGCGGCCCTG GTGGTCCCGG AGGACCAAAT GGACCTTACG GACCTGGTGG TCCCGGAGGA 
CCAAATGGAC CTTACGGACC CGGCGGTACT GGCGGACCTA ATGGTCCTTA TGGCCCAGGT 
GGTCAAGATG GCTCATACAG ACCAGACGGC TCCTACGGTC CAGACGGATC TTATCAACCG 
GAAGGTTCCT ACGGACCCGA CGGATCATGG CAAGGTGGTT ACTATCCTGG CACTCCTGGA 
TCGCCTGGAA CTCCTGGGTC GCCTGGCAGC CCTGGATCCC CTGGTAGCCC GGGTACTCCT 
GGTTCTCCAG GCACACCAGG AACACCTGGT TCAGGCGGTG GATACTATCC TGGACAAGAC 
GGAACAGGGG GACCGACTGG TCCTTCAGGC GGGGGAGGTG GCTACTATCC AGGTAGTCCC 
GGCAGTCCTG GAACCCCCGG CACTCCAGGC ACACCCGGAA CACCAGGTGG CCCTGGGGGT 
CCAGGTGGAC CAGGTGGACC AGGTGGTCCA GGAGGGCCAA ACGGTCCAAA TGGGCCTGGA 
GGATCTGACG GCGGTCAATA TTATCCTGCA CTGCCCGGTA GTCCCGGTAC ACCCGGCTCC 
CCAGGCACTC CTGGTGGACC TGGTGGACCT GGTGGTCCCG GAGGCCCTGG GGGACCAGGC 
GGCCCAGGCG GCCCGGGAGG TCCAGATCAA CCAAGCTACC CTGGACAGCC AGGACAGCCT 
GGTACACCAG GATATCCAGG ACAACCAGGA TACCCAGGAC AACCCGGTAC ACCAGGACAA 
CCAGGAACAC CAGGCCAGCC AGGCTATCCA GGACAAGGTG GACAACCTAT AAAGCCTGGT 
CAGCCAGGAT ACCCAGGTCA GCCAGGCCAG CCGGGATACC CAGGACAACC AGGACAGCCA 
GGAACACCAG GACAGCCAGG AACACCAGGA CAGCCAGGAA CACCAGGCCA GCCAGGAACA 
CCAGGACAAC CAGGACAAGG TGGACAACCT ATAAAGCCTG CTCAGCCGGG ACACCCAGGC 
CAGCCTGGAC AACCAGGGCA GCCCGGAACA CCAGGACAAC CAGGTCAACC AGGATACCCA 
GGGCAACCGG GTCAGCCAGG ACAACCAGGG CAACCAGGAC AACAAGGCTA TCCAGGACAA 
GGTGGACCAC AACAGGGCGG TCAGCCTATT AATCCTAGTC AACCTGGTTA CCCAGGCCAG 
TCAGGACAAC CAGGTTACCC TGGACAACCG GTTCAGCCAG GACAACCAGG GCAGCCCGGA 
ACACCAGGGG AACCGGGACA GCCCGGTACA CCAGGACAAC CTGGCTATCC AGGACAAGGT 
GGACCACAAC AGGGCGGTCA GCCTATGAAT CCTAGTCAAC CCGAATACCC AAGCCAGCCA 
GGACAACCAG GACAGCCAGG AACACCGGGA CAAGCAGGAC ACCCAGGACA ACCTGGACAC 
CCAGGCGCAC CAGGACAACC AGGTTACCCA GGACAACCCG GACAGACGGG CGCACCAGGA 
CAAGCAGGTT ACCCAGGACA AGGTGGACCG CAACAGGGCG GTCAGCCTAT AAATCCTAGT 
CAACCAGGAC ATCCAGTGCA GCCAGGACAG CCGGGTCAAC CAGGGCAGCC AGGAAAACCC 
GGGCAACCTG GTACACCAGG GCAGCCTGGA GCTCCAGGGC AACCTGGAAC ACCAGGACAA 
CCAGGCTATC CAGGACAAAA TGGACCACAA CAAGGCGGTC AGCCTATTAA ACCTGATCAA 
CCCGGATACC CAGGCCAACC GGGAGCACCA GGACAGCCAG GAACACCAGG ACAACCCGGA 
TATCCAGGAC AAGGCGGACC ACAACAGGGC GGTCAGCCTA TCAATCCCGA TCAGCCCGGA 
CAGCCAGGTC CACCAGGATA TCCAGGGCAA GGCGGACAAC CCATAAATCC TAGTCAATCA 
GGCTACCCGG GACAACCAGG ACAAGGAGGC GATTCCGTCT CGTCGGGAAC TGGTGTGCAG 
CCACCGACAT ATGTTCCATC AACTCAAATG CCACCTTTCC CAATTTATGT CATACCTTTT 
CCATTGCCTA TCGTACCAAG TCCAGGATCA TGTCCCTGCT ATTTATTAAA TCCTGGAAAT 
AACGGTACAG CAACGAACGA AATGCAAGCA TCCTCACATC CCGCAAATGG ACAGAGTCAA 
GGATATGCTC CGTACGGTAT TATAGGATTC ATACCTGTTG TTTTCGTTCC CTATTGTCCA 
GGAAATGGGA GTGCGATGAA CACTGCACAA CAAAACTTCC CTCAAGCAGT ACCGGTGCAA 
TACAATTGTG CCCAATGTCA GGCCAATCGA GATGTCTACA GATTCGCAGG CCGAAACAAT 
GGCGCCAGAG CGTTCAAAGA GCTTAAAGAA CTTAAATCGC TGGACGAACT TAACGATCTC 
ATTCGAAATA GAATAAAACC TACCACGAGG ACATTACGTC AAATTGCAGC ACATCCAAAA 
ATACTTGAAA CAAAACACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021207436.1
CDS	176..3436
Translation	MGPGIMIKFCILAVLALSLVSADKPKTETTSTTAKKATEVPAKKTVEVPLKKASEVPVKKASEVPTKKPHVEPAREGRQLSPYPGQQDNEVVVDIQDDEKKQYYETNYDTSAYGFGYDVGPNGQFHHENRGPDGVVYGCYGYVDTEGYLRATHYVADSHGYRVVEPEKPVEVFPDEKYEYDENYEPTPSATGQTIPWNKLFFPQGCGRTPGGVPPQPFKPKPKPKPPVDSTGQTSNPSPGVAYPGQGPNGPYGPGGSGGSGGPGGPGGPNGPYGPGGPGGPNGPYGPGGTGGPNGPYGPGGQDGSYRPDGSYGPDGSYQPEGSYGPDGSWQGGYYPGTPGSPGTPGSPGSPGSPGSPGTPGSPGTPGTPGSGGGYYPGQDGTGGPTGPSGGGGGYYPGSPGSPGTPGTPGTPGTPGGPGGPGGPGGPGGPGGPNGPNGPGGSDGGQYYPALPGSPGTPGSPGTPGGPGGPGGPGGPGGPGGPGGPGGPDQPSYPGQPGQPGTPGYPGQPGYPGQPGTPGQPGTPGQPGYPGQGGQPIKPGQPGYPGQPGQPGYPGQPGQPGTPGQPGTPGQPGTPGQPGTPGQPGQGGQPIKPAQPGHPGQPGQPGQPGTPGQPGQPGYPGQPGQPGQPGQPGQQGYPGQGGPQQGGQPINPSQPGYPGQSGQPGYPGQPVQPGQPGQPGTPGEPGQPGTPGQPGYPGQGGPQQGGQPMNPSQPEYPSQPGQPGQPGTPGQAGHPGQPGHPGAPGQPGYPGQPGQTGAPGQAGYPGQGGPQQGGQPINPSQPGHPVQPGQPGQPGQPGKPGQPGTPGQPGAPGQPGTPGQPGYPGQNGPQQGGQPIKPDQPGYPGQPGAPGQPGTPGQPGYPGQGGPQQGGQPINPDQPGQPGPPGYPGQGGQPINPSQSGYPGQPGQGGDSVSSGTGVQPPTYVPSTQMPPFPIYVIPFPLPIVPSPGSCPCYLLNPGNNGTATNEMQASSHPANGQSQGYAPYGIIGFIPVVFVPYCPGNGSAMNTAQQNFPQAVPVQYNCAQCQANRDVYRFAGRNNGARAFKELKELKSLDELNDLIRNRIKPTTRTLRQIAAHPKILETKH

Target ORF information:

RefSeq Version	XM_021351761.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori collagen (CPR146), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021351761.1

ORF Insert Sequence:

ATGGGACCCG GAATAATGAT TAAATTTTGT ATCTTGGCGG TGCTAGCATT GTCACTGGTT 
AGCGCCGATA AGCCAAAAAC TGAGACAACT TCAACGACCG CGAAAAAGGC AACTGAAGTA 
CCCGCGAAGA AGACAGTCGA AGTACCTTTG AAGAAGGCAT CTGAAGTGCC CGTGAAGAAG 
GCATCAGAAG TGCCAACAAA GAAGCCCCAT GTAGAACCCG CGCGTGAAGG AAGGCAGTTG 
AGTCCTTACC CTGGACAACA AGACAACGAA GTGGTGGTCG ACATTCAGGA CGACGAAAAG 
AAGCAGTACT ACGAAACCAA CTATGACACA AGTGCGTATG GTTTCGGATA CGATGTCGGC 
CCAAACGGAC AGTTTCATCA CGAGAACCGC GGTCCCGATG GTGTCGTCTA CGGATGTTAT 
GGTTACGTCG ACACCGAGGG CTACCTCCGC GCCACCCACT ACGTTGCCGA CAGCCACGGC 
TACCGAGTCG TAGAACCAGA AAAACCTGTC GAAGTTTTCC CCGATGAGAA ATACGAATAT 
GACGAAAATT ACGAACCCAC GCCATCCGCG ACGGGTCAAA CCATTCCGTG GAATAAGTTA 
TTCTTCCCCC AGGGATGCGG TCGCACTCCT GGCGGAGTTC CACCCCAACC GTTCAAACCA 
AAACCTAAAC CTAAACCCCC TGTAGATAGC ACCGGACAAA CTAGCAACCC CAGTCCTGGC 
GTCGCATACC CTGGTCAAGG CCCTAATGGC CCTTACGGTC CTGGCGGCTC CGGCGGCTCC 
GGCGGCCCTG GTGGTCCCGG AGGACCAAAT GGACCTTACG GACCTGGTGG TCCCGGAGGA 
CCAAATGGAC CTTACGGACC CGGCGGTACT GGCGGACCTA ATGGTCCTTA TGGCCCAGGT 
GGTCAAGATG GCTCATACAG ACCAGACGGC TCCTACGGTC CAGACGGATC TTATCAACCG 
GAAGGTTCCT ACGGACCCGA CGGATCATGG CAAGGTGGTT ACTATCCTGG CACTCCTGGA 
TCGCCTGGAA CTCCTGGGTC GCCTGGCAGC CCTGGATCCC CTGGTAGCCC GGGTACTCCT 
GGTTCTCCAG GCACACCAGG AACACCTGGT TCAGGCGGTG GATACTATCC TGGACAAGAC 
GGAACAGGGG GACCGACTGG TCCTTCAGGC GGGGGAGGTG GCTACTATCC AGGTAGTCCC 
GGCAGTCCTG GAACCCCCGG CACTCCAGGC ACACCCGGAA CACCAGGTGG CCCTGGGGGT 
CCAGGTGGAC CAGGTGGACC AGGTGGTCCA GGAGGGCCAA ACGGTCCAAA TGGGCCTGGA 
GGATCTGACG GCGGTCAATA TTATCCTGCA CTGCCCGGTA GTCCCGGTAC ACCCGGCTCC 
CCAGGCACTC CTGGTGGACC TGGTGGACCT GGTGGTCCCG GAGGCCCTGG GGGACCAGGC 
GGCCCAGGCG GCCCGGGAGG TCCAGATCAA CCAAGCTACC CTGGACAGCC AGGACAGCCT 
GGTACACCAG GATATCCAGG ACAACCAGGA TACCCAGGAC AACCCGGTAC ACCAGGACAA 
CCAGGAACAC CAGGCCAGCC AGGCTATCCA GGACAAGGTG GACAACCTAT AAAGCCTGGT 
CAGCCAGGAT ACCCAGGTCA GCCAGGCCAG CCGGGATACC CAGGACAACC AGGACAGCCA 
GGAACACCAG GACAGCCAGG AACACCAGGA CAGCCAGGAA CACCAGGCCA GCCAGGAACA 
CCAGGACAAC CAGGACAAGG TGGACAACCT ATAAAGCCTG CTCAGCCGGG ACACCCAGGC 
CAGCCTGGAC AACCAGGGCA GCCCGGAACA CCAGGACAAC CAGGTCAACC AGGATACCCA 
GGGCAACCGG GTCAGCCAGG ACAACCAGGG CAACCAGGAC AACAAGGCTA TCCAGGACAA 
GGTGGACCAC AACAGGGCGG TCAGCCTATT AATCCTAGTC AACCTGGTTA CCCAGGCCAG 
TCAGGACAAC CAGGTTACCC TGGACAACCG GTTCAGCCAG GACAACCAGG GCAGCCCGGA 
ACACCAGGGG AACCGGGACA GCCCGGTACA CCAGGACAAC CTGGCTATCC AGGACAAGGT 
GGACCACAAC AGGGCGGTCA GCCTATGAAT CCTAGTCAAC CCGAATACCC AAGCCAGCCA 
GGACAACCAG GACAGCCAGG AACACCGGGA CAAGCAGGAC ACCCAGGACA ACCTGGACAC 
CCAGGCGCAC CAGGACAACC AGGTTACCCA GGACAACCCG GACAGACGGG CGCACCAGGA 
CAAGCAGGTT ACCCAGGACA AGGTGGACCG CAACAGGGCG GTCAGCCTAT AAATCCTAGT 
CAACCAGGAC ATCCAGTGCA GCCAGGACAG CCGGGTCAAC CAGGGCAGCC AGGAAAACCC 
GGGCAACCTG GTACACCAGG GCAGCCTGGA GCTCCAGGGC AACCTGGAAC ACCAGGACAA 
CCAGGCTATC CAGGACAAAA TGGACCACAA CAAGGCGGTC AGCCTATTAA ACCTGATCAA 
CCCGGATACC CAGGCCAACC GGGAGCACCA GGACAGCCAG GAACACCAGG ACAACCCGGA 
TATCCAGGAC AAGGCGGACC ACAACAGGGC GGTCAGCCTA TCAATCCCGA TCAGCCCGGA 
CAGCCAGGTC CACCAGGATA TCCAGGGCAA GGCGGACAAC CCATAAATCC TAGTCAATCA 
GGCTACCCGG GACAACCAGG ACAAGGAGGC GATTCCGTCT CGTCGGGAAC TGGTGTGCAG 
CCACCGACAT ATGTTCCATC AACTCAAATG CCACCTTTCC CAATTTATGT CATACCTTTT 
CCATTGCCTA TCGTACCAAG TCCAGGATCA TGTCCCTGCT ATTTATTAAA TCCTGGAAAT 
AACGGTACAG CAACGAACGA AATGCAAGCA TCCTCACATC CCGCAAATGG ACAGAGTCAA 
GGATATGCTC CGTACGGTAT TATAGGATTC ATACCTGTTG TTTTCGTTCC CTATTGTCCA 
GGAAATGGGA GTGCGATGAA CACTGCACAA CAAAACTTCC CTCAAGCAGT ACCGGTGCAA 
TACAATTGTG CCCAATGTCA GGCCAATCGA GATGTCTACA GATTCGCAGG CCGAAACAAT 
GGCGCCAGAG CGTTCAAAGA GCTTAAAGAA CTTAAATCGC TGGACGAACT TAACGATCTC 
ATTCGAAATA GAATAAAACC TACCACGAGG ACATTACGTC AAATTGCAGC ACATCCAAAA 
ATACTTGAAA CAAAACACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPR146 ( XM_021351762.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_021207437.1 Bombyx mori collagen (CPR146), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBg27468
Clone ID Related Accession (Same CDS sequence)	XM_021351762.1
Accession Version	XM_021351762.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1496505600000
Organism	Bombyx mori(domestic silkworm)
Product	collagen isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004582025.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCCG GAATAATGAT TAAATTTTGT ATCTTGGCGG TGCTAGCATT GTCACTGGTT 
AGCGCCGATA AGCCAAAAAC TGAGACAACT TCAACGACCG CGAAAAAGGC AACTGAAGTA 
CCCGCGAAGA AGACAGTCGA AGTACCTTTG AAGAAGGCAT CTGAAGTGCC CGTGAAGAAG 
GCATCAGAAG TGCCAACAAA GAAGCCCCAT GTAGAACCCG CGCGTGAAGG AAGGCAGTTG 
AGTCCTTACC CTGGACAACA AGACAACGAA GTGGTGGTCG ACATTCAGGA CGACGAAAAG 
AAGCAGTACT ACGAAACCAA CTATGACACA AGTGCGTATG GTTTCGGATA CGATGTCGGC 
CCAAACGGAC AGTTTCATCA CGAGAACCGC GGTCCCGATG GTGTCGTCTA CGGATGTTAT 
GGTTACGTCG ACACCGAGGG CTACCTCCGC GCCACCCACT ACGTTGCCGA CAGCCACGGC 
TACCGAGTCG TAGAACCAGA AAAACCTGTC GAAGTTTTCC CCGATGAGAA ATACGAATAT 
GACGAAAATT ACGAACCCAC GCCATCCGCG ACGGGTCAAA CCATTCCGTG GAATAAGTTA 
TTCTTCCCCC AGGGATGCGG TCGCACTCCT GGCGGAGTTC CACCCCAACC GTTCAAACCA 
AAACCTAAAC CTAAACCCCC TGTAGATAGC ACCGGACAAA CTAGCAACCC CAGTCCTGGC 
GTCGCATACC CTGGTCAAGG CCCTAATGGC CCTTACGGTC CTGGCGGCTC CGGCGGCTCC 
GGCGGCCCTG GTGGTCCCGG AGGACCAAAT GGACCTTACG GACCTGGTGG TCCCGGAGGA 
CCAAATGGAC CTTACGGACC CGGCGGTACT GGCGGACCTA ATGGTCCTTA TGGCCCAGGT 
GGTCAAGATG GCTCATACAG ACCAGACGGC TCCTACGGTC CAGACGGATC TTATCAACCG 
GAAGGTTCCT ACGGACCCGA CGGATCATGG CAAGGTGGTT ACTATCCTGG CACTCCTGGA 
TCGCCTGGAA CTCCTGGGTC GCCTGGCAGC CCTGGATCCC CTGGTAGCCC GGGTACTCCT 
GGTTCTCCAG GCACACCAGG AACACCTGGT TCAGGCGGTG GATACTATCC TGGACAAGAC 
GGAACAGGGG GACCGACTGG TCCTTCAGGC GGGGGAGGTG GCTACTATCC AGGTAGTCCC 
GGCAGTCCTG GAACCCCCGG CACTCCAGGC ACACCCGGAA CACCAGGTGG CCCTGGGGGT 
CCAGGTGGAC CAGGTGGACC AGGTGGTCCA GGAGGGCCAA ACGGTCCAAA TGGGCCTGGA 
GGATCTGACG GCGGTCAATA TTATCCTGCA CTGCCCGGTA GTCCCGGTAC ACCCGGCTCC 
CCAGGCACTC CTGGTGGACC TGGTGGACCT GGTGGTCCCG GAGGCCCTGG GGGACCAGGC 
GGCCCAGGCG GCCCGGGAGG TCCAGATCAA CCAAGCTACC CTGGACAGCC AGGACAGCCT 
GGTACACCAG GATATCCAGG ACAACCAGGA TACCCAGGAC AACCCGGTAC ACCAGGACAA 
CCAGGAACAC CAGGCCAGCC AGGCTATCCA GGACAAGGTG GACAACCTAT AAAGCCTGGT 
CAGCCAGGAT ACCCAGGTCA GCCAGGCCAG CCGGGATACC CAGGACAACC AGGACAGCCA 
GGAACACCAG GACAGCCAGG AACACCAGGA CAGCCAGGAA CACCAGGCCA GCCAGGAACA 
CCAGGACAAC CAGGGCAGCC CGGAACACCA GGACAACCAG GTCAACCAGG ATACCCAGGG 
CAACCGGGTC AGCCAGGACA ACCAGGGCAA CCAGGACAAC AAGGCTATCC AGGACAAGGT 
GGACCACAAC AGGGCGGTCA GCCTATTAAT CCTAGTCAAC CTGGTTACCC AGGCCAGTCA 
GGACAACCAG GTTACCCTGG ACAACCGGTT CAGCCAGGAC AACCAGGGCA GCCCGGAACA 
CCAGGGGAAC CGGGACAGCC CGGTACACCA GGACAACCTG GCTATCCAGG ACAAGGTGGA 
CCACAACAGG GCGGTCAGCC TATGAATCCT AGTCAACCCG AATACCCAAG CCAGCCAGGA 
CAACCAGGAC AGCCAGGAAC ACCGGGACAA GCAGGACACC CAGGACAACC TGGACACCCA 
GGCGCACCAG GACAACCAGG TTACCCAGGA CAACCCGGAC AGACGGGCGC ACCAGGACAA 
GCAGGTTACC CAGGACAAGG TGGACCGCAA CAGGGCGGTC AGCCTATAAA TCCTAGTCAA 
CCAGGACATC CAGTGCAGCC AGGACAGCCG GGTCAACCAG GGCAGCCAGG AAAACCCGGG 
CAACCTGGTA CACCAGGGCA GCCTGGAGCT CCAGGGCAAC CTGGAACACC AGGACAACCA 
GGCTATCCAG GACAAAATGG ACCACAACAA GGCGGTCAGC CTATTAAACC TGATCAACCC 
GGATACCCAG GCCAACCGGG AGCACCAGGA CAGCCAGGAA CACCAGGACA ACCCGGATAT 
CCAGGACAAG GCGGACCACA ACAGGGCGGT CAGCCTATCA ATCCCGATCA GCCCGGACAG 
CCAGGTCCAC CAGGATATCC AGGGCAAGGC GGACAACCCA TAAATCCTAG TCAATCAGGC 
TACCCGGGAC AACCAGGACA AGGAGGCGAT TCCGTCTCGT CGGGAACTGG TGTGCAGCCA 
CCGACATATG TTCCATCAAC TCAAATGCCA CCTTTCCCAA TTTATGTCAT ACCTTTTCCA 
TTGCCTATCG TACCAAGTCC AGGATCATGT CCCTGCTATT TATTAAATCC TGGAAATAAC 
GGTACAGCAA CGAACGAAAT GCAAGCATCC TCACATCCCG CAAATGGACA GAGTCAAGGA 
TATGCTCCGT ACGGTATTAT AGGATTCATA CCTGTTGTTT TCGTTCCCTA TTGTCCAGGA 
AATGGGAGTG CGATGAACAC TGCACAACAA AACTTCCCTC AAGCAGTACC GGTGCAATAC 
AATTGTGCCC AATGTCAGGC CAATCGAGAT GTCTACAGAT TCGCAGGCCG AAACAATGGC 
GCCAGAGCGT TCAAAGAGCT TAAAGAACTT AAATCGCTGG ACGAACTTAA CGATCTCATT 
CGAAATAGAA TAAAACCTAC CACGAGGACA TTACGTCAAA TTGCAGCACA TCCAAAAATA 
CTTGAAACAA AACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021207437.1
CDS	176..3373
Translation	MGPGIMIKFCILAVLALSLVSADKPKTETTSTTAKKATEVPAKKTVEVPLKKASEVPVKKASEVPTKKPHVEPAREGRQLSPYPGQQDNEVVVDIQDDEKKQYYETNYDTSAYGFGYDVGPNGQFHHENRGPDGVVYGCYGYVDTEGYLRATHYVADSHGYRVVEPEKPVEVFPDEKYEYDENYEPTPSATGQTIPWNKLFFPQGCGRTPGGVPPQPFKPKPKPKPPVDSTGQTSNPSPGVAYPGQGPNGPYGPGGSGGSGGPGGPGGPNGPYGPGGPGGPNGPYGPGGTGGPNGPYGPGGQDGSYRPDGSYGPDGSYQPEGSYGPDGSWQGGYYPGTPGSPGTPGSPGSPGSPGSPGTPGSPGTPGTPGSGGGYYPGQDGTGGPTGPSGGGGGYYPGSPGSPGTPGTPGTPGTPGGPGGPGGPGGPGGPGGPNGPNGPGGSDGGQYYPALPGSPGTPGSPGTPGGPGGPGGPGGPGGPGGPGGPGGPDQPSYPGQPGQPGTPGYPGQPGYPGQPGTPGQPGTPGQPGYPGQGGQPIKPGQPGYPGQPGQPGYPGQPGQPGTPGQPGTPGQPGTPGQPGTPGQPGQPGTPGQPGQPGYPGQPGQPGQPGQPGQQGYPGQGGPQQGGQPINPSQPGYPGQSGQPGYPGQPVQPGQPGQPGTPGEPGQPGTPGQPGYPGQGGPQQGGQPMNPSQPEYPSQPGQPGQPGTPGQAGHPGQPGHPGAPGQPGYPGQPGQTGAPGQAGYPGQGGPQQGGQPINPSQPGHPVQPGQPGQPGQPGKPGQPGTPGQPGAPGQPGTPGQPGYPGQNGPQQGGQPIKPDQPGYPGQPGAPGQPGTPGQPGYPGQGGPQQGGQPINPDQPGQPGPPGYPGQGGQPINPSQSGYPGQPGQGGDSVSSGTGVQPPTYVPSTQMPPFPIYVIPFPLPIVPSPGSCPCYLLNPGNNGTATNEMQASSHPANGQSQGYAPYGIIGFIPVVFVPYCPGNGSAMNTAQQNFPQAVPVQYNCAQCQANRDVYRFAGRNNGARAFKELKELKSLDELNDLIRNRIKPTTRTLRQIAAHPKILETKH

Target ORF information:

RefSeq Version	XM_021351762.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori collagen (CPR146), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021351762.1

ORF Insert Sequence:

ATGGGACCCG GAATAATGAT TAAATTTTGT ATCTTGGCGG TGCTAGCATT GTCACTGGTT 
AGCGCCGATA AGCCAAAAAC TGAGACAACT TCAACGACCG CGAAAAAGGC AACTGAAGTA 
CCCGCGAAGA AGACAGTCGA AGTACCTTTG AAGAAGGCAT CTGAAGTGCC CGTGAAGAAG 
GCATCAGAAG TGCCAACAAA GAAGCCCCAT GTAGAACCCG CGCGTGAAGG AAGGCAGTTG 
AGTCCTTACC CTGGACAACA AGACAACGAA GTGGTGGTCG ACATTCAGGA CGACGAAAAG 
AAGCAGTACT ACGAAACCAA CTATGACACA AGTGCGTATG GTTTCGGATA CGATGTCGGC 
CCAAACGGAC AGTTTCATCA CGAGAACCGC GGTCCCGATG GTGTCGTCTA CGGATGTTAT 
GGTTACGTCG ACACCGAGGG CTACCTCCGC GCCACCCACT ACGTTGCCGA CAGCCACGGC 
TACCGAGTCG TAGAACCAGA AAAACCTGTC GAAGTTTTCC CCGATGAGAA ATACGAATAT 
GACGAAAATT ACGAACCCAC GCCATCCGCG ACGGGTCAAA CCATTCCGTG GAATAAGTTA 
TTCTTCCCCC AGGGATGCGG TCGCACTCCT GGCGGAGTTC CACCCCAACC GTTCAAACCA 
AAACCTAAAC CTAAACCCCC TGTAGATAGC ACCGGACAAA CTAGCAACCC CAGTCCTGGC 
GTCGCATACC CTGGTCAAGG CCCTAATGGC CCTTACGGTC CTGGCGGCTC CGGCGGCTCC 
GGCGGCCCTG GTGGTCCCGG AGGACCAAAT GGACCTTACG GACCTGGTGG TCCCGGAGGA 
CCAAATGGAC CTTACGGACC CGGCGGTACT GGCGGACCTA ATGGTCCTTA TGGCCCAGGT 
GGTCAAGATG GCTCATACAG ACCAGACGGC TCCTACGGTC CAGACGGATC TTATCAACCG 
GAAGGTTCCT ACGGACCCGA CGGATCATGG CAAGGTGGTT ACTATCCTGG CACTCCTGGA 
TCGCCTGGAA CTCCTGGGTC GCCTGGCAGC CCTGGATCCC CTGGTAGCCC GGGTACTCCT 
GGTTCTCCAG GCACACCAGG AACACCTGGT TCAGGCGGTG GATACTATCC TGGACAAGAC 
GGAACAGGGG GACCGACTGG TCCTTCAGGC GGGGGAGGTG GCTACTATCC AGGTAGTCCC 
GGCAGTCCTG GAACCCCCGG CACTCCAGGC ACACCCGGAA CACCAGGTGG CCCTGGGGGT 
CCAGGTGGAC CAGGTGGACC AGGTGGTCCA GGAGGGCCAA ACGGTCCAAA TGGGCCTGGA 
GGATCTGACG GCGGTCAATA TTATCCTGCA CTGCCCGGTA GTCCCGGTAC ACCCGGCTCC 
CCAGGCACTC CTGGTGGACC TGGTGGACCT GGTGGTCCCG GAGGCCCTGG GGGACCAGGC 
GGCCCAGGCG GCCCGGGAGG TCCAGATCAA CCAAGCTACC CTGGACAGCC AGGACAGCCT 
GGTACACCAG GATATCCAGG ACAACCAGGA TACCCAGGAC AACCCGGTAC ACCAGGACAA 
CCAGGAACAC CAGGCCAGCC AGGCTATCCA GGACAAGGTG GACAACCTAT AAAGCCTGGT 
CAGCCAGGAT ACCCAGGTCA GCCAGGCCAG CCGGGATACC CAGGACAACC AGGACAGCCA 
GGAACACCAG GACAGCCAGG AACACCAGGA CAGCCAGGAA CACCAGGCCA GCCAGGAACA 
CCAGGACAAC CAGGGCAGCC CGGAACACCA GGACAACCAG GTCAACCAGG ATACCCAGGG 
CAACCGGGTC AGCCAGGACA ACCAGGGCAA CCAGGACAAC AAGGCTATCC AGGACAAGGT 
GGACCACAAC AGGGCGGTCA GCCTATTAAT CCTAGTCAAC CTGGTTACCC AGGCCAGTCA 
GGACAACCAG GTTACCCTGG ACAACCGGTT CAGCCAGGAC AACCAGGGCA GCCCGGAACA 
CCAGGGGAAC CGGGACAGCC CGGTACACCA GGACAACCTG GCTATCCAGG ACAAGGTGGA 
CCACAACAGG GCGGTCAGCC TATGAATCCT AGTCAACCCG AATACCCAAG CCAGCCAGGA 
CAACCAGGAC AGCCAGGAAC ACCGGGACAA GCAGGACACC CAGGACAACC TGGACACCCA 
GGCGCACCAG GACAACCAGG TTACCCAGGA CAACCCGGAC AGACGGGCGC ACCAGGACAA 
GCAGGTTACC CAGGACAAGG TGGACCGCAA CAGGGCGGTC AGCCTATAAA TCCTAGTCAA 
CCAGGACATC CAGTGCAGCC AGGACAGCCG GGTCAACCAG GGCAGCCAGG AAAACCCGGG 
CAACCTGGTA CACCAGGGCA GCCTGGAGCT CCAGGGCAAC CTGGAACACC AGGACAACCA 
GGCTATCCAG GACAAAATGG ACCACAACAA GGCGGTCAGC CTATTAAACC TGATCAACCC 
GGATACCCAG GCCAACCGGG AGCACCAGGA CAGCCAGGAA CACCAGGACA ACCCGGATAT 
CCAGGACAAG GCGGACCACA ACAGGGCGGT CAGCCTATCA ATCCCGATCA GCCCGGACAG 
CCAGGTCCAC CAGGATATCC AGGGCAAGGC GGACAACCCA TAAATCCTAG TCAATCAGGC 
TACCCGGGAC AACCAGGACA AGGAGGCGAT TCCGTCTCGT CGGGAACTGG TGTGCAGCCA 
CCGACATATG TTCCATCAAC TCAAATGCCA CCTTTCCCAA TTTATGTCAT ACCTTTTCCA 
TTGCCTATCG TACCAAGTCC AGGATCATGT CCCTGCTATT TATTAAATCC TGGAAATAAC 
GGTACAGCAA CGAACGAAAT GCAAGCATCC TCACATCCCG CAAATGGACA GAGTCAAGGA 
TATGCTCCGT ACGGTATTAT AGGATTCATA CCTGTTGTTT TCGTTCCCTA TTGTCCAGGA 
AATGGGAGTG CGATGAACAC TGCACAACAA AACTTCCCTC AAGCAGTACC GGTGCAATAC 
AATTGTGCCC AATGTCAGGC CAATCGAGAT GTCTACAGAT TCGCAGGCCG AAACAATGGC 
GCCAGAGCGT TCAAAGAGCT TAAAGAACTT AAATCGCTGG ACGAACTTAA CGATCTCATT 
CGAAATAGAA TAAAACCTAC CACGAGGACA TTACGTCAAA TTGCAGCACA TCCAAAAATA 
CTTGAAACAA AACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPR146 ( XM_021351763.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_021207438.1 Bombyx mori collagen (CPR146), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBg27469
Clone ID Related Accession (Same CDS sequence)	XM_021351763.1
Accession Version	XM_021351763.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1496505600000
Organism	Bombyx mori(domestic silkworm)
Product	collagen isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004582025.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCCG GAATAATGAT TAAATTTTGT ATCTTGGCGG TGCTAGCATT GTCACTGGTT 
AGCGCCGATA AGCCAAAAAC TGAGACAACT TCAACGACCG CGAAAAAGGC AACTGAAGTA 
CCCGCGAAGA AGACAGTCGA AGTACCTTTG AAGAAGGCAT CTGAAGTGCC CGTGAAGAAG 
GCATCAGAAG TGCCAACAAA GAAGCCCCAT GTAGAACCCG CGCGTGAAGG AAGGCAGTTG 
AGTCCTTACC CTGGACAACA AGACAACGAA GTGGTGGTCG ACATTCAGGA CGACGAAAAG 
AAGCAGTACT ACGAAACCAA CTATGACACA AGTGCGTATG GTTTCGGATA CGATGTCGGC 
CCAAACGGAC AGTTTCATCA CGAGAACCGC GGTCCCGATG GTGTCGTCTA CGGATGTTAT 
GGTTACGTCG ACACCGAGGG CTACCTCCGC GCCACCCACT ACGTTGCCGA CAGCCACGGC 
TACCGAGTCG TAGAACCAGA AAAACCTGTC GAAGTTTTCC CCGATGAGAA ATACGAATAT 
GACGAAAATT ACGAACCCAC GCCATCCGCG ACGGGTCAAA CCATTCCGTG GAATAAGTTA 
TTCTTCCCCC AGGGATGCGG TCGCACTCCT GGCGGAGTTC CACCCCAACC GTTCAAACCA 
AAACCTAAAC CTAAACCCCC TGTAGATAGC ACCGGACAAA CTAGCAACCC CAGTCCTGGC 
GTCGCATACC CTGGTCAAGG CCCTAATGGC CCTTACGGTC CTGGCGGCTC CGGCGGCTCC 
GGCGGCCCTG GTGGTCCCGG AGGACCAAAT GGACCTTACG GACCTGGTGG TCCCGGAGGA 
CCAAATGGAC CTTACGGACC CGGCGGTACT GGCGGACCTA ATGGTCCTTA TGGCCCAGGT 
GGTCAAGATG GCTCATACAG ACCAGACGGC TCCTACGGTC CAGACGGATC TTATCAACCG 
GAAGGTTCCT ACGGACCCGA CGGATCATGG CAAGGTGGTT ACTATCCTGG CACTCCTGGA 
TCGCCTGGAA CTCCTGGGTC GCCTGGCAGC CCTGGATCCC CTGGTAGCCC GGGTACTCCT 
GGTTCTCCAG GCACACCAGG AACACCTGGT TCAGGCGGTG GATACTATCC TGGACAAGAC 
GGAACAGGGG GACCGACTGG TCCTTCAGGC GGGGGAGGTG GCTACTATCC AGGTAGTCCC 
GGCAGTCCTG GAACCCCCGG CACTCCAGGC ACACCCGGAA CACCAGGTGG CCCTGGGGGT 
CCAGGTGGAC CAGGTGGACC AGGTGGTCCA GGAGGGCCAA ACGGTCCAAA TGGGCCTGGA 
GGATCTGACG GCGGTCAATA TTATCCTGCA CTGCCCGGTA GTCCCGGTAC ACCCGGCTCC 
CCAGGCACTC CTGGTGGACC TGGTGGACCT GGTGGTCCCG GAGGCCCTGG GGGACCAGGC 
GGCCCAGGCG GCCCGGGAGG TCCAGATCAA CCAAGCTACC CTGGACAGCC AGGACAGCCT 
GGTACACCAG GATATCCAGG ACAACCAGGA TACCCAGGAC AACCCGGTAC ACCAGGACAA 
CCAGGAACAC CAGGCCAGCC AGGCTATCCA GGACAAGGTG GACAACCTAT AAAGCCTGGT 
CAGCCAGGAT ACCCAGGTCA GCCAGGCCAG CCGGGATACC CAGGACAACC AGGACAGCCA 
GGAACACCAG GACAGCCAGG AACACCAGGA CAGCCAGGAA CACCAGGCCA GCCAGGAACA 
CCAGGACAAC CAGGCCAGCC CGGAACACCA GGACAACCAG GTCAACCAGG CTACCCAGGA 
CAAGGTGGAC AGCCTATAAA GCCTGGTCAG CCAGGATACC CAGGTCAGCC AGGACAAGGT 
GGACCACAAC AGGGCGGTCA GCCTATTAAT CCTAGTCAAC CTGGTTACCC AGGCCAGTCA 
GGACAACCAG GTTACCCTGG ACAACCGGTT CAGCCAGGAC AACCAGGGCA GCCCGGAACA 
CCAGGGGAAC CGGGACAGCC CGGTACACCA GGACAACCTG GCTATCCAGG ACAAGGTGGA 
CCACAACAGG GCGGTCAGCC TATGAATCCT AGTCAACCCG AATACCCAAG CCAGCCAGGA 
CAACCAGGAC AGCCAGGAAC ACCGGGACAA GCAGGACACC CAGGACAACC TGGACACCCA 
GGCGCACCAG GACAACCAGG TTACCCAGGA CAACCCGGAC AGACGGGCGC ACCAGGACAA 
GCAGGTTACC CAGGACAAGG TGGACCGCAA CAGGGCGGTC AGCCTATAAA TCCTAGTCAA 
CCAGGACATC CAGTGCAGCC AGGACAGCCG GGTCAACCAG GGCAGCCAGG AAAACCCGGG 
CAACCTGGTA CACCAGGGCA GCCTGGAGCT CCAGGGCAAC CTGGAACACC AGGACAACCA 
GGCTATCCAG GACAAAATGG ACCACAACAA GGCGGTCAGC CTATTAAACC TGATCAACCC 
GGATACCCAG GCCAACCGGG AGCACCAGGA CAGCCAGGAA CACCAGGACA ACCCGGATAT 
CCAGGACAAG GCGGACCACA ACAGGGCGGT CAGCCTATCA ATCCCGATCA GCCCGGACAG 
CCAGGTCCAC CAGGATATCC AGGGCAAGGC GGACAACCCA TAAATCCTAG TCAATCAGGC 
TACCCGGGAC AACCAGGACA AGGAGGCGAT TCCGTCTCGT CGGGAACTGG TGTGCAGCCA 
CCGACATATG TTCCATCAAC TCAAATGCCA CCTTTCCCAA TTTATGTCAT ACCTTTTCCA 
TTGCCTATCG TACCAAGTCC AGGATCATGT CCCTGCTATT TATTAAATCC TGGAAATAAC 
GGTACAGCAA CGAACGAAAT GCAAGCATCC TCACATCCCG CAAATGGACA GAGTCAAGGA 
TATGCTCCGT ACGGTATTAT AGGATTCATA CCTGTTGTTT TCGTTCCCTA TTGTCCAGGA 
AATGGGAGTG CGATGAACAC TGCACAACAA AACTTCCCTC AAGCAGTACC GGTGCAATAC 
AATTGTGCCC AATGTCAGGC CAATCGAGAT GTCTACAGAT TCGCAGGCCG AAACAATGGC 
GCCAGAGCGT TCAAAGAGCT TAAAGAACTT AAATCGCTGG ACGAACTTAA CGATCTCATT 
CGAAATAGAA TAAAACCTAC CACGAGGACA TTACGTCAAA TTGCAGCACA TCCAAAAATA 
CTTGAAACAA AACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021207438.1
CDS	176..3373
Translation	MGPGIMIKFCILAVLALSLVSADKPKTETTSTTAKKATEVPAKKTVEVPLKKASEVPVKKASEVPTKKPHVEPAREGRQLSPYPGQQDNEVVVDIQDDEKKQYYETNYDTSAYGFGYDVGPNGQFHHENRGPDGVVYGCYGYVDTEGYLRATHYVADSHGYRVVEPEKPVEVFPDEKYEYDENYEPTPSATGQTIPWNKLFFPQGCGRTPGGVPPQPFKPKPKPKPPVDSTGQTSNPSPGVAYPGQGPNGPYGPGGSGGSGGPGGPGGPNGPYGPGGPGGPNGPYGPGGTGGPNGPYGPGGQDGSYRPDGSYGPDGSYQPEGSYGPDGSWQGGYYPGTPGSPGTPGSPGSPGSPGSPGTPGSPGTPGTPGSGGGYYPGQDGTGGPTGPSGGGGGYYPGSPGSPGTPGTPGTPGTPGGPGGPGGPGGPGGPGGPNGPNGPGGSDGGQYYPALPGSPGTPGSPGTPGGPGGPGGPGGPGGPGGPGGPGGPDQPSYPGQPGQPGTPGYPGQPGYPGQPGTPGQPGTPGQPGYPGQGGQPIKPGQPGYPGQPGQPGYPGQPGQPGTPGQPGTPGQPGTPGQPGTPGQPGQPGTPGQPGQPGYPGQGGQPIKPGQPGYPGQPGQGGPQQGGQPINPSQPGYPGQSGQPGYPGQPVQPGQPGQPGTPGEPGQPGTPGQPGYPGQGGPQQGGQPMNPSQPEYPSQPGQPGQPGTPGQAGHPGQPGHPGAPGQPGYPGQPGQTGAPGQAGYPGQGGPQQGGQPINPSQPGHPVQPGQPGQPGQPGKPGQPGTPGQPGAPGQPGTPGQPGYPGQNGPQQGGQPIKPDQPGYPGQPGAPGQPGTPGQPGYPGQGGPQQGGQPINPDQPGQPGPPGYPGQGGQPINPSQSGYPGQPGQGGDSVSSGTGVQPPTYVPSTQMPPFPIYVIPFPLPIVPSPGSCPCYLLNPGNNGTATNEMQASSHPANGQSQGYAPYGIIGFIPVVFVPYCPGNGSAMNTAQQNFPQAVPVQYNCAQCQANRDVYRFAGRNNGARAFKELKELKSLDELNDLIRNRIKPTTRTLRQIAAHPKILETKH

Target ORF information:

RefSeq Version	XM_021351763.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori collagen (CPR146), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021351763.1

ORF Insert Sequence:

ATGGGACCCG GAATAATGAT TAAATTTTGT ATCTTGGCGG TGCTAGCATT GTCACTGGTT 
AGCGCCGATA AGCCAAAAAC TGAGACAACT TCAACGACCG CGAAAAAGGC AACTGAAGTA 
CCCGCGAAGA AGACAGTCGA AGTACCTTTG AAGAAGGCAT CTGAAGTGCC CGTGAAGAAG 
GCATCAGAAG TGCCAACAAA GAAGCCCCAT GTAGAACCCG CGCGTGAAGG AAGGCAGTTG 
AGTCCTTACC CTGGACAACA AGACAACGAA GTGGTGGTCG ACATTCAGGA CGACGAAAAG 
AAGCAGTACT ACGAAACCAA CTATGACACA AGTGCGTATG GTTTCGGATA CGATGTCGGC 
CCAAACGGAC AGTTTCATCA CGAGAACCGC GGTCCCGATG GTGTCGTCTA CGGATGTTAT 
GGTTACGTCG ACACCGAGGG CTACCTCCGC GCCACCCACT ACGTTGCCGA CAGCCACGGC 
TACCGAGTCG TAGAACCAGA AAAACCTGTC GAAGTTTTCC CCGATGAGAA ATACGAATAT 
GACGAAAATT ACGAACCCAC GCCATCCGCG ACGGGTCAAA CCATTCCGTG GAATAAGTTA 
TTCTTCCCCC AGGGATGCGG TCGCACTCCT GGCGGAGTTC CACCCCAACC GTTCAAACCA 
AAACCTAAAC CTAAACCCCC TGTAGATAGC ACCGGACAAA CTAGCAACCC CAGTCCTGGC 
GTCGCATACC CTGGTCAAGG CCCTAATGGC CCTTACGGTC CTGGCGGCTC CGGCGGCTCC 
GGCGGCCCTG GTGGTCCCGG AGGACCAAAT GGACCTTACG GACCTGGTGG TCCCGGAGGA 
CCAAATGGAC CTTACGGACC CGGCGGTACT GGCGGACCTA ATGGTCCTTA TGGCCCAGGT 
GGTCAAGATG GCTCATACAG ACCAGACGGC TCCTACGGTC CAGACGGATC TTATCAACCG 
GAAGGTTCCT ACGGACCCGA CGGATCATGG CAAGGTGGTT ACTATCCTGG CACTCCTGGA 
TCGCCTGGAA CTCCTGGGTC GCCTGGCAGC CCTGGATCCC CTGGTAGCCC GGGTACTCCT 
GGTTCTCCAG GCACACCAGG AACACCTGGT TCAGGCGGTG GATACTATCC TGGACAAGAC 
GGAACAGGGG GACCGACTGG TCCTTCAGGC GGGGGAGGTG GCTACTATCC AGGTAGTCCC 
GGCAGTCCTG GAACCCCCGG CACTCCAGGC ACACCCGGAA CACCAGGTGG CCCTGGGGGT 
CCAGGTGGAC CAGGTGGACC AGGTGGTCCA GGAGGGCCAA ACGGTCCAAA TGGGCCTGGA 
GGATCTGACG GCGGTCAATA TTATCCTGCA CTGCCCGGTA GTCCCGGTAC ACCCGGCTCC 
CCAGGCACTC CTGGTGGACC TGGTGGACCT GGTGGTCCCG GAGGCCCTGG GGGACCAGGC 
GGCCCAGGCG GCCCGGGAGG TCCAGATCAA CCAAGCTACC CTGGACAGCC AGGACAGCCT 
GGTACACCAG GATATCCAGG ACAACCAGGA TACCCAGGAC AACCCGGTAC ACCAGGACAA 
CCAGGAACAC CAGGCCAGCC AGGCTATCCA GGACAAGGTG GACAACCTAT AAAGCCTGGT 
CAGCCAGGAT ACCCAGGTCA GCCAGGCCAG CCGGGATACC CAGGACAACC AGGACAGCCA 
GGAACACCAG GACAGCCAGG AACACCAGGA CAGCCAGGAA CACCAGGCCA GCCAGGAACA 
CCAGGACAAC CAGGCCAGCC CGGAACACCA GGACAACCAG GTCAACCAGG CTACCCAGGA 
CAAGGTGGAC AGCCTATAAA GCCTGGTCAG CCAGGATACC CAGGTCAGCC AGGACAAGGT 
GGACCACAAC AGGGCGGTCA GCCTATTAAT CCTAGTCAAC CTGGTTACCC AGGCCAGTCA 
GGACAACCAG GTTACCCTGG ACAACCGGTT CAGCCAGGAC AACCAGGGCA GCCCGGAACA 
CCAGGGGAAC CGGGACAGCC CGGTACACCA GGACAACCTG GCTATCCAGG ACAAGGTGGA 
CCACAACAGG GCGGTCAGCC TATGAATCCT AGTCAACCCG AATACCCAAG CCAGCCAGGA 
CAACCAGGAC AGCCAGGAAC ACCGGGACAA GCAGGACACC CAGGACAACC TGGACACCCA 
GGCGCACCAG GACAACCAGG TTACCCAGGA CAACCCGGAC AGACGGGCGC ACCAGGACAA 
GCAGGTTACC CAGGACAAGG TGGACCGCAA CAGGGCGGTC AGCCTATAAA TCCTAGTCAA 
CCAGGACATC CAGTGCAGCC AGGACAGCCG GGTCAACCAG GGCAGCCAGG AAAACCCGGG 
CAACCTGGTA CACCAGGGCA GCCTGGAGCT CCAGGGCAAC CTGGAACACC AGGACAACCA 
GGCTATCCAG GACAAAATGG ACCACAACAA GGCGGTCAGC CTATTAAACC TGATCAACCC 
GGATACCCAG GCCAACCGGG AGCACCAGGA CAGCCAGGAA CACCAGGACA ACCCGGATAT 
CCAGGACAAG GCGGACCACA ACAGGGCGGT CAGCCTATCA ATCCCGATCA GCCCGGACAG 
CCAGGTCCAC CAGGATATCC AGGGCAAGGC GGACAACCCA TAAATCCTAG TCAATCAGGC 
TACCCGGGAC AACCAGGACA AGGAGGCGAT TCCGTCTCGT CGGGAACTGG TGTGCAGCCA 
CCGACATATG TTCCATCAAC TCAAATGCCA CCTTTCCCAA TTTATGTCAT ACCTTTTCCA 
TTGCCTATCG TACCAAGTCC AGGATCATGT CCCTGCTATT TATTAAATCC TGGAAATAAC 
GGTACAGCAA CGAACGAAAT GCAAGCATCC TCACATCCCG CAAATGGACA GAGTCAAGGA 
TATGCTCCGT ACGGTATTAT AGGATTCATA CCTGTTGTTT TCGTTCCCTA TTGTCCAGGA 
AATGGGAGTG CGATGAACAC TGCACAACAA AACTTCCCTC AAGCAGTACC GGTGCAATAC 
AATTGTGCCC AATGTCAGGC CAATCGAGAT GTCTACAGAT TCGCAGGCCG AAACAATGGC 
GCCAGAGCGT TCAAAGAGCT TAAAGAACTT AAATCGCTGG ACGAACTTAA CGATCTCATT 
CGAAATAGAA TAAAACCTAC CACGAGGACA TTACGTCAAA TTGCAGCACA TCCAAAAATA 
CTTGAAACAA AACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPR146 ( NM_001171643.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> NP_001165114.1 Bombyx mori collagen (CPR146), mRNA.

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CloneID	OBg13562
Clone ID Related Accession (Same CDS sequence)	NM_001171643.1
Accession Version	NM_001171643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556812800000
Organism	Bombyx mori(domestic silkworm)
Product	collagen precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BR000647.1. ##Evidence-Data-START## Transcript exon combination :: BR000647.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3450056, SAMEA3450057 [ECO:0000350] ##Evidence-Data-END##

ATGGGACCCG GAATAATGAT TAAATTTTGT ATCTTGGCGG TGCTAGCATT GTCACTGGTT 
AGCGCCGATA AGCCAAAAAC TGAGACAACT TCAACGACCG CGAAAAAGGC AACTGAAGTA 
CCCGCGAAGA AGACAGTCGA AGTACCTTTG AAGAAGGCAT CTGAAGTGCC CGTGAAGAAG 
GCATCAGAAG TGCCAACAAA GAAGCCCCAT GTAGAACCCG CGCGTGAAGG AAGGCAGTTG 
AGTCCTTACC CTGGACAACA AGACAACGAA GTGGTGGTCG ACATTCAGGA CGACGAAAAG 
AAGCAGTACT ACGAAACCAA CTATGACACA AGTGCGTATG GTTTCGGATA CGATGTCGGC 
CCAAACGGAC AGTTTCATCA CGAGAACCGC GGTCCCGATG GTGTCGTCTA CGGATGTTAT 
GGTTACGTCG ACACCGAGGG CTACCTCCGC GCCACCCACT ACGTTGCCGA CAGCCACGGC 
TACCGAGTCG TAGAACCAGA AAAACCTGTC GAAGTTTTCC CCGATGAGAA ATACGAATAT 
GACGAAAATT ACGAACCCAC GCCATCCGCG ACGGGTCAAA CCATTCCGTG GAATAAGTTA 
TTCTTCCCCC AGGGATGCGG TCGCACTCCT GGCGGAGTTC CACCCCAACC GTTCAAACCA 
AAACCTAAAC CTAAACCCCC TGTAGATAGC ACCGGACAAA CTAGCAACCC CAGTCCTGGC 
GTCGCATACC CTGGTCAAGG CCCTAATGGC CCTTACGGTC CTGGCGGCTC CGGCGGCTCC 
GGCGGCCCTG GTGGTCCCGG AGGACCAAAT GGACCTTACG GACCTGGTGG TCCCGGAGGA 
CCAAATGGAC CTTACGGACC CGGCGGTACT GGCGGACCTA ATGGTCCTTA TGGCCCAGGT 
GGTCAAGATG GCTCATACAG ACCAGACGGC TCCTACGGTC CAGACGGATC TTATCAACCG 
GAAGGTTCCT ACGGACCCGA CGGATCATGG CAAGGTGGTT ACTATCCTGG CACTCCTGGA 
TCGCCTGGAA CTCCTGGGTC GCCTGGCAGC CCTGGATCCC CTGGTAGCCC GGGTACTCCT 
GGTTCTCCAG GCACACCAGG AACACCTGGT TCAGGCGGTG GATACTATCC TGGACAAGAC 
GGAACAGGGG GACCGACTGG TCCTTCAGGC GGGGGAGGTG GCTACTATCC AGGTAGTCCC 
GGCAGTCCTG GAACCCCCGG CACTCCAGGC ACACCCGGAA CACCAGGTGG CCCTGGGGGT 
CCAGGTGGAC CAGGTGGACC AGGTGGTCCA GGAGGGCCAA ACGGTCCAAA TGGGCCTGGA 
GGATCTGACG GCGGTCAATA TTATCCTGCA CTGCCCGGTA GTCCCGGTAC ACCCGGCTCC 
CCAGGCACTC CTGGTGGACC TGGTGGACCT GGTGGTCCCG GAGGCCCTGG GGGACCAGGC 
GGCCCAGGCG GCCCGGGAGG TCCAGATCAA CCAAGCTACC CTGGACAGCC AGGACAGCCT 
GGTACACCAG GATATCCAGG ACAACCAGGA TACCCAGGAC AACCCGGTAC ACCAGGACAA 
CCAGGAACAC CAGGCCAGCC AGGCTATCCA GGACAAGGTG GACAACCTAT AAATCCTGGT 
CAACCAGGAT ACCCAGGCCA GCCCGGACAA CCAGGATACC CGGGACAACC AGGTCAGCCA 
GGAACACCAG GACAACCCGG AACGCCTGGA CAACCAGGAA CGCCAGGACA ACCAGGACAA 
CCCGGCTATC CAGGAACTCC TGGGCAACCA GGGCAATCCG GATATCCAGG ACAGGGTGGA 
CCACAACAGG GAGGATACCC AGGTCAGCCA GGCCAGCCGG GATACCCAGG ACAACCAGGA 
CAGCCAGGAA CACCAGGACA GCCAGGAACA CCAGGACAGC CAGGAACACC AGGCCAGCCA 
GGAACACCAG GACAACCAGG TCAACCAGGC TACCCAGGAC AAGCTGGACA GCCTGTAAAT 
CCTGCTCAGC CGGGATACCC AGGACAGCCA GGTCAGCCAG GATACCCAGG ACAACCAGGA 
CAGCCAGGAA CTCCAGGACA GCCAGGAACA CCAGGACAGC CCGGAACACC AGGACAACCA 
GGTCAACCAG GCTACCCAGG ACAAGCTGGA CAGCCTGTAA ATCCTGCTCA GCCGGGATAC 
CCAGGTCAGC CAAGTCAGCC GGGATACCCA GGACAACCAG GACAGCCAGG AACACCAGGA 
CAGCCAGGAA CACCAGGACA ACCAGGGCAG CCCGGAACAC CAGGACAACC AGGTCAACCA 
GGCTACCCAG GACAAGGTGG ACAGCCTATA AAGCCTGGTC AGACAGGATA CCCAGGTCAG 
CCAGGCCAGC CGGGATACCC AGGACAACCA GGCCAGCCAG GAACACCAGG ACAGCCAGGA 
ACACCAGGAC AGCCAGGCCA ACCAGGCTAC CCAGGACAAG GTGGACAGCC TATAAAGCCT 
GGTCAGACAG GATACCCAGG TCAGCCAGGC CAGCCGGGAT ACCCAGGACA ACCAGGACAG 
CCAGGAACAC CAGGACAGCC AGGCCAACCG GGACAACCCG GTACACCAGG CCAGCCCGGA 
ACACCAGGAC AACCAGGTCA ACCAGGCTAC CCAGGACAAG GTGGACAGCC TGTAAATCCT 
GCTCAGCCGG GATACCCAGG ACAGCCAGGT CAGCCAGGAT ACCCAGGACA ACCAGGACAG 
CCAGGAACTC CAGGACAGCC AGGAACACCA GGACAGCCCG GAACACCAGG ACAACCAGGT 
CAACCAGGCT ACCCAGGACA AGGTGGACAG CCTGTAAATC CTGCTCAGCC GGGATACCCA 
GGTCAGCCAA GTCAGCCGGG ATACCCAGGA CAACTAGGAC AACCAGGAAC TCCAGGACAA 
CCAGGACAGC CCGGAACACC AGGACAGCCC GGAACACCAG GACAACCAGG TCAACCAGGC 
TACCCAGGAC AAGGTGGACA GCCTATAAAT CCTACTCAGC CGGGATCCCC AGGTCAGCTA 
GGTCAGCCAG GATACCCAGG ACAACCAGGA CAGCCAGCAG GACAACCAGG CTATCCCGGA 
CAAGGTGGAC AACAACAGGG CGGTCAGCCC ATTAATCCTA GTCAACCTGG ATACCCCAGC 
CATCCAGGAC AACCAGGATA CCCAGGACAA CCAGGTCAAC CAGGACAACC AGGCCAGCCC 
GGAACACCAG GACAACCAGG TCAACCAGGC TACCCAGGAC AAGGTGGACA GCCTATAAAG 
CCTGGTCAGC CAGGATACCC AGGTCAGCCA GGCCAGCCGG GATACCCTGG ACAACCAGGA 
CAGCCAGGAG CACCAGGCCA ACCGGGTCAG CCAGGACAAC CAGTGCAGCC CGGAACACCA 
GGACAAGCAG GTCAACCAGG CTACCCAGGA CAAGGTGGAC AACCTATAAA GCCTGCTCAG 
CCGGGACACC CAGGCCAGCC TGGACAACCA GGGCAGCCCG GAACACCAGG ACAACCAGGT 
CAACCAGGAT ACCCAGGGCA ACCGGGTCAG CCAGGACAAC CAGGGCAACC AGGACAACAA 
GGCTATCCAG GACAAGGTGG ACCACAACAG GGCGGTCAGC CTATTAATCC TAGTCAACCT 
GGTTACCCAG GCCAGTCAGG ACAACCAGGT TACCCTGGAC AACCGGTTCA GCCAGGACAA 
CCAGGGCAGC CCGGAACACC AGGGGAACCG GGACAGCCCG GTACACCAGG ACAACCTGGC 
TATCCAGGAC AAGGTGGACC ACAACAGGGC GGTCAGCCTA TGAATCCTAG TCAACCCGAA 
TACCCAAGCC AGCCAGGACA ACCAGGACAG CCAGGAACAC CGGGACAAGC AGGACACCCA 
GGACAACCTG GACACCCAGG CGCACCAGGA CAACCAGGTT ACCCAGGACA ACCCGGACAG 
ACGGGCGCAC CAGGACAAGC AGGTTACCCA GGACAAGGTG GACCGCAACA GGGCGGTCAG 
CCTATAAATC CTAGTCAACC AGGACATCCA GTGCAGCCAG GACAGCCGGG TCAACCAGGG 
CAGCCAGGAA AACCCGGGCA ACCTGGTACA CCAGGGCAGC CTGGAGCTCC AGGGCAACCT 
GGAACACCAG GACAACCAGG CTATCCAGGA CAAAATGGAC CACAACAAGG CGGTCAGCCT 
ATTAAACCTG ATCAACCCGG ATACCCAGGC CAACCGGGAG CACCAGGGCA GCCTGGAACA 
CCAGGACAAC CCGGACAGCC AGGAACACCA GGACAACCCG GATATCCAGG ACAAGGCGGA 
CCACAACAGG GCGGTCAGCC TATCAATCCC GATCAGCCCG GACAGCCAGG TCCACCAGGA 
TATCCAGGGC AAGGCGGACA ACCCATAAAT CCTAGTCAAT CAGGCTACCC GGGACAACCA 
GGACAAGGAG GCGATTCCGT CTCGTCGGGA ACTGGTGTGC AGCCACCGAC ATATGTTCCA 
TCAACTCAAA TGCCACCTTT CCCAATTTAT GTCATACCTT TTCCATTGCC TATCGTACCA 
AGTCCAGGAT CATGTCCCTG CTATTTATTA AATCCTGGAA ATAACGGTAC AGCAACGAAC 
GAAATGCAAG CATCCTCACA TCCCGCAAAT GGACAGAGTC AAGGATATGC TCCGTACGGT 
ATTATAGGAT TCATACCTGT TGTTTTCGTT CCCTATTGTC CAGGAAATGG GAGTGCGATG 
AACACTGCAC AACAAAACTT CCCTCAAGCA GTACCGGTGC AATACAATTG TGCCCAATGT 
CAGGCCAATC GAGATGTCTA CAGATTCGCA GGCCGAAACA ATGGCGCCAG AGCGTTCAAA 
GAGCTTAAAG AACTTAAATC GCTGGACGAA CTTAACGATC TCATTCGAAA TAGAATAAAA 
CCTACCACGA GGACATTACG TCAAATTGCA GCACATCCAA AAATACTTGA AACAAAACAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165114.1
CDS	125..5047
Translation	MGPGIMIKFCILAVLALSLVSADKPKTETTSTTAKKATEVPAKKTVEVPLKKASEVPVKKASEVPTKKPHVEPAREGRQLSPYPGQQDNEVVVDIQDDEKKQYYETNYDTSAYGFGYDVGPNGQFHHENRGPDGVVYGCYGYVDTEGYLRATHYVADSHGYRVVEPEKPVEVFPDEKYEYDENYEPTPSATGQTIPWNKLFFPQGCGRTPGGVPPQPFKPKPKPKPPVDSTGQTSNPSPGVAYPGQGPNGPYGPGGSGGSGGPGGPGGPNGPYGPGGPGGPNGPYGPGGTGGPNGPYGPGGQDGSYRPDGSYGPDGSYQPEGSYGPDGSWQGGYYPGTPGSPGTPGSPGSPGSPGSPGTPGSPGTPGTPGSGGGYYPGQDGTGGPTGPSGGGGGYYPGSPGSPGTPGTPGTPGTPGGPGGPGGPGGPGGPGGPNGPNGPGGSDGGQYYPALPGSPGTPGSPGTPGGPGGPGGPGGPGGPGGPGGPGGPDQPSYPGQPGQPGTPGYPGQPGYPGQPGTPGQPGTPGQPGYPGQGGQPINPGQPGYPGQPGQPGYPGQPGQPGTPGQPGTPGQPGTPGQPGQPGYPGTPGQPGQSGYPGQGGPQQGGYPGQPGQPGYPGQPGQPGTPGQPGTPGQPGTPGQPGTPGQPGQPGYPGQAGQPVNPAQPGYPGQPGQPGYPGQPGQPGTPGQPGTPGQPGTPGQPGQPGYPGQAGQPVNPAQPGYPGQPSQPGYPGQPGQPGTPGQPGTPGQPGQPGTPGQPGQPGYPGQGGQPIKPGQTGYPGQPGQPGYPGQPGQPGTPGQPGTPGQPGQPGYPGQGGQPIKPGQTGYPGQPGQPGYPGQPGQPGTPGQPGQPGQPGTPGQPGTPGQPGQPGYPGQGGQPVNPAQPGYPGQPGQPGYPGQPGQPGTPGQPGTPGQPGTPGQPGQPGYPGQGGQPVNPAQPGYPGQPSQPGYPGQLGQPGTPGQPGQPGTPGQPGTPGQPGQPGYPGQGGQPINPTQPGSPGQLGQPGYPGQPGQPAGQPGYPGQGGQQQGGQPINPSQPGYPSHPGQPGYPGQPGQPGQPGQPGTPGQPGQPGYPGQGGQPIKPGQPGYPGQPGQPGYPGQPGQPGAPGQPGQPGQPVQPGTPGQAGQPGYPGQGGQPIKPAQPGHPGQPGQPGQPGTPGQPGQPGYPGQPGQPGQPGQPGQQGYPGQGGPQQGGQPINPSQPGYPGQSGQPGYPGQPVQPGQPGQPGTPGEPGQPGTPGQPGYPGQGGPQQGGQPMNPSQPEYPSQPGQPGQPGTPGQAGHPGQPGHPGAPGQPGYPGQPGQTGAPGQAGYPGQGGPQQGGQPINPSQPGHPVQPGQPGQPGQPGKPGQPGTPGQPGAPGQPGTPGQPGYPGQNGPQQGGQPIKPDQPGYPGQPGAPGQPGTPGQPGQPGTPGQPGYPGQGGPQQGGQPINPDQPGQPGPPGYPGQGGQPINPSQSGYPGQPGQGGDSVSSGTGVQPPTYVPSTQMPPFPIYVIPFPLPIVPSPGSCPCYLLNPGNNGTATNEMQASSHPANGQSQGYAPYGIIGFIPVVFVPYCPGNGSAMNTAQQNFPQAVPVQYNCAQCQANRDVYRFAGRNNGARAFKELKELKSLDELNDLIRNRIKPTTRTLRQIAAHPKILETKH

Target ORF information:

RefSeq Version	NM_001171643.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori collagen (CPR146), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171643.1

ORF Insert Sequence:

ATGGGACCCG GAATAATGAT TAAATTTTGT ATCTTGGCGG TGCTAGCATT GTCACTGGTT 
AGCGCCGATA AGCCAAAAAC TGAGACAACT TCAACGACCG CGAAAAAGGC AACTGAAGTA 
CCCGCGAAGA AGACAGTCGA AGTACCTTTG AAGAAGGCAT CTGAAGTGCC CGTGAAGAAG 
GCATCAGAAG TGCCAACAAA GAAGCCCCAT GTAGAACCCG CGCGTGAAGG AAGGCAGTTG 
AGTCCTTACC CTGGACAACA AGACAACGAA GTGGTGGTCG ACATTCAGGA CGACGAAAAG 
AAGCAGTACT ACGAAACCAA CTATGACACA AGTGCGTATG GTTTCGGATA CGATGTCGGC 
CCAAACGGAC AGTTTCATCA CGAGAACCGC GGTCCCGATG GTGTCGTCTA CGGATGTTAT 
GGTTACGTCG ACACCGAGGG CTACCTCCGC GCCACCCACT ACGTTGCCGA CAGCCACGGC 
TACCGAGTCG TAGAACCAGA AAAACCTGTC GAAGTTTTCC CCGATGAGAA ATACGAATAT 
GACGAAAATT ACGAACCCAC GCCATCCGCG ACGGGTCAAA CCATTCCGTG GAATAAGTTA 
TTCTTCCCCC AGGGATGCGG TCGCACTCCT GGCGGAGTTC CACCCCAACC GTTCAAACCA 
AAACCTAAAC CTAAACCCCC TGTAGATAGC ACCGGACAAA CTAGCAACCC CAGTCCTGGC 
GTCGCATACC CTGGTCAAGG CCCTAATGGC CCTTACGGTC CTGGCGGCTC CGGCGGCTCC 
GGCGGCCCTG GTGGTCCCGG AGGACCAAAT GGACCTTACG GACCTGGTGG TCCCGGAGGA 
CCAAATGGAC CTTACGGACC CGGCGGTACT GGCGGACCTA ATGGTCCTTA TGGCCCAGGT 
GGTCAAGATG GCTCATACAG ACCAGACGGC TCCTACGGTC CAGACGGATC TTATCAACCG 
GAAGGTTCCT ACGGACCCGA CGGATCATGG CAAGGTGGTT ACTATCCTGG CACTCCTGGA 
TCGCCTGGAA CTCCTGGGTC GCCTGGCAGC CCTGGATCCC CTGGTAGCCC GGGTACTCCT 
GGTTCTCCAG GCACACCAGG AACACCTGGT TCAGGCGGTG GATACTATCC TGGACAAGAC 
GGAACAGGGG GACCGACTGG TCCTTCAGGC GGGGGAGGTG GCTACTATCC AGGTAGTCCC 
GGCAGTCCTG GAACCCCCGG CACTCCAGGC ACACCCGGAA CACCAGGTGG CCCTGGGGGT 
CCAGGTGGAC CAGGTGGACC AGGTGGTCCA GGAGGGCCAA ACGGTCCAAA TGGGCCTGGA 
GGATCTGACG GCGGTCAATA TTATCCTGCA CTGCCCGGTA GTCCCGGTAC ACCCGGCTCC 
CCAGGCACTC CTGGTGGACC TGGTGGACCT GGTGGTCCCG GAGGCCCTGG GGGACCAGGC 
GGCCCAGGCG GCCCGGGAGG TCCAGATCAA CCAAGCTACC CTGGACAGCC AGGACAGCCT 
GGTACACCAG GATATCCAGG ACAACCAGGA TACCCAGGAC AACCCGGTAC ACCAGGACAA 
CCAGGAACAC CAGGCCAGCC AGGCTATCCA GGACAAGGTG GACAACCTAT AAATCCTGGT 
CAACCAGGAT ACCCAGGCCA GCCCGGACAA CCAGGATACC CGGGACAACC AGGTCAGCCA 
GGAACACCAG GACAACCCGG AACGCCTGGA CAACCAGGAA CGCCAGGACA ACCAGGACAA 
CCCGGCTATC CAGGAACTCC TGGGCAACCA GGGCAATCCG GATATCCAGG ACAGGGTGGA 
CCACAACAGG GAGGATACCC AGGTCAGCCA GGCCAGCCGG GATACCCAGG ACAACCAGGA 
CAGCCAGGAA CACCAGGACA GCCAGGAACA CCAGGACAGC CAGGAACACC AGGCCAGCCA 
GGAACACCAG GACAACCAGG TCAACCAGGC TACCCAGGAC AAGCTGGACA GCCTGTAAAT 
CCTGCTCAGC CGGGATACCC AGGACAGCCA GGTCAGCCAG GATACCCAGG ACAACCAGGA 
CAGCCAGGAA CTCCAGGACA GCCAGGAACA CCAGGACAGC CCGGAACACC AGGACAACCA 
GGTCAACCAG GCTACCCAGG ACAAGCTGGA CAGCCTGTAA ATCCTGCTCA GCCGGGATAC 
CCAGGTCAGC CAAGTCAGCC GGGATACCCA GGACAACCAG GACAGCCAGG AACACCAGGA 
CAGCCAGGAA CACCAGGACA ACCAGGGCAG CCCGGAACAC CAGGACAACC AGGTCAACCA 
GGCTACCCAG GACAAGGTGG ACAGCCTATA AAGCCTGGTC AGACAGGATA CCCAGGTCAG 
CCAGGCCAGC CGGGATACCC AGGACAACCA GGCCAGCCAG GAACACCAGG ACAGCCAGGA 
ACACCAGGAC AGCCAGGCCA ACCAGGCTAC CCAGGACAAG GTGGACAGCC TATAAAGCCT 
GGTCAGACAG GATACCCAGG TCAGCCAGGC CAGCCGGGAT ACCCAGGACA ACCAGGACAG 
CCAGGAACAC CAGGACAGCC AGGCCAACCG GGACAACCCG GTACACCAGG CCAGCCCGGA 
ACACCAGGAC AACCAGGTCA ACCAGGCTAC CCAGGACAAG GTGGACAGCC TGTAAATCCT 
GCTCAGCCGG GATACCCAGG ACAGCCAGGT CAGCCAGGAT ACCCAGGACA ACCAGGACAG 
CCAGGAACTC CAGGACAGCC AGGAACACCA GGACAGCCCG GAACACCAGG ACAACCAGGT 
CAACCAGGCT ACCCAGGACA AGGTGGACAG CCTGTAAATC CTGCTCAGCC GGGATACCCA 
GGTCAGCCAA GTCAGCCGGG ATACCCAGGA CAACTAGGAC AACCAGGAAC TCCAGGACAA 
CCAGGACAGC CCGGAACACC AGGACAGCCC GGAACACCAG GACAACCAGG TCAACCAGGC 
TACCCAGGAC AAGGTGGACA GCCTATAAAT CCTACTCAGC CGGGATCCCC AGGTCAGCTA 
GGTCAGCCAG GATACCCAGG ACAACCAGGA CAGCCAGCAG GACAACCAGG CTATCCCGGA 
CAAGGTGGAC AACAACAGGG CGGTCAGCCC ATTAATCCTA GTCAACCTGG ATACCCCAGC 
CATCCAGGAC AACCAGGATA CCCAGGACAA CCAGGTCAAC CAGGACAACC AGGCCAGCCC 
GGAACACCAG GACAACCAGG TCAACCAGGC TACCCAGGAC AAGGTGGACA GCCTATAAAG 
CCTGGTCAGC CAGGATACCC AGGTCAGCCA GGCCAGCCGG GATACCCTGG ACAACCAGGA 
CAGCCAGGAG CACCAGGCCA ACCGGGTCAG CCAGGACAAC CAGTGCAGCC CGGAACACCA 
GGACAAGCAG GTCAACCAGG CTACCCAGGA CAAGGTGGAC AACCTATAAA GCCTGCTCAG 
CCGGGACACC CAGGCCAGCC TGGACAACCA GGGCAGCCCG GAACACCAGG ACAACCAGGT 
CAACCAGGAT ACCCAGGGCA ACCGGGTCAG CCAGGACAAC CAGGGCAACC AGGACAACAA 
GGCTATCCAG GACAAGGTGG ACCACAACAG GGCGGTCAGC CTATTAATCC TAGTCAACCT 
GGTTACCCAG GCCAGTCAGG ACAACCAGGT TACCCTGGAC AACCGGTTCA GCCAGGACAA 
CCAGGGCAGC CCGGAACACC AGGGGAACCG GGACAGCCCG GTACACCAGG ACAACCTGGC 
TATCCAGGAC AAGGTGGACC ACAACAGGGC GGTCAGCCTA TGAATCCTAG TCAACCCGAA 
TACCCAAGCC AGCCAGGACA ACCAGGACAG CCAGGAACAC CGGGACAAGC AGGACACCCA 
GGACAACCTG GACACCCAGG CGCACCAGGA CAACCAGGTT ACCCAGGACA ACCCGGACAG 
ACGGGCGCAC CAGGACAAGC AGGTTACCCA GGACAAGGTG GACCGCAACA GGGCGGTCAG 
CCTATAAATC CTAGTCAACC AGGACATCCA GTGCAGCCAG GACAGCCGGG TCAACCAGGG 
CAGCCAGGAA AACCCGGGCA ACCTGGTACA CCAGGGCAGC CTGGAGCTCC AGGGCAACCT 
GGAACACCAG GACAACCAGG CTATCCAGGA CAAAATGGAC CACAACAAGG CGGTCAGCCT 
ATTAAACCTG ATCAACCCGG ATACCCAGGC CAACCGGGAG CACCAGGGCA GCCTGGAACA 
CCAGGACAAC CCGGACAGCC AGGAACACCA GGACAACCCG GATATCCAGG ACAAGGCGGA 
CCACAACAGG GCGGTCAGCC TATCAATCCC GATCAGCCCG GACAGCCAGG TCCACCAGGA 
TATCCAGGGC AAGGCGGACA ACCCATAAAT CCTAGTCAAT CAGGCTACCC GGGACAACCA 
GGACAAGGAG GCGATTCCGT CTCGTCGGGA ACTGGTGTGC AGCCACCGAC ATATGTTCCA 
TCAACTCAAA TGCCACCTTT CCCAATTTAT GTCATACCTT TTCCATTGCC TATCGTACCA 
AGTCCAGGAT CATGTCCCTG CTATTTATTA AATCCTGGAA ATAACGGTAC AGCAACGAAC 
GAAATGCAAG CATCCTCACA TCCCGCAAAT GGACAGAGTC AAGGATATGC TCCGTACGGT 
ATTATAGGAT TCATACCTGT TGTTTTCGTT CCCTATTGTC CAGGAAATGG GAGTGCGATG 
AACACTGCAC AACAAAACTT CCCTCAAGCA GTACCGGTGC AATACAATTG TGCCCAATGT 
CAGGCCAATC GAGATGTCTA CAGATTCGCA GGCCGAAACA ATGGCGCCAG AGCGTTCAAA 
GAGCTTAAAG AACTTAAATC GCTGGACGAA CTTAACGATC TCATTCGAAA TAGAATAAAA 
CCTACCACGA GGACATTACG TCAAATTGCA GCACATCCAA AAATACTTGA AACAAAACAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPR146 ( XM_012695260.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_012550714.1 Bombyx mori collagen (CPR146), transcript variant X1, mRNA.

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CloneID	OBg14076
Clone ID Related Accession (Same CDS sequence)	XM_012695260.1
Accession Version	XM_012695260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433347200000
Organism	Bombyx mori(Domestic silkworm)
Product	collagen isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004582025.1) and transcript sequences (BR000647.1 and GBJR01000301.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 2 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGGACCCG GAATAATGAT TAAATTTTGT ATCTTGGCGG TGCTAGCATT GTCACTGGTT 
AGCGCCGATA AGCCAAAAAC TGAGACAACT TCAACGACCG CGAAAAAGGC AACTGAAGTA 
CCCGCGAAGA AGACAGTCGA AGTACCTTTG AAGAAGGCAT CTGAAGTGCC CGTGAAGAAG 
GCATCAGAAG TGCCAACAAA GAAGCCCCAT GTAGAACCCG CGCGTGAAGG AAGGCAGTTG 
AGTCCTTACC CTGGACAACA AGACAACGAA GTGGTGGTCG ACATTCAGGA CGACGAAAAG 
AAGCAGTACT ACGAAACCAA CTATGACACA AGTGCGTATG GTTTCGGATA CGATGTCGGC 
CCAAACGGAC AGTTTCATCA CGAGAACCGC GGTCCCGATG GTGTCGTCTA CGGATGTTAT 
GGTTACGTCG ACACCGAGGG CTACCTCCGC GCCACCCACT ACGTTGCCGA CAGCCACGGC 
TACCGAGTCG TAGAACCAGA AAAACCTGTC GAAGTTTTCC CCGATGAGAA ATACGAATAT 
GACGAAAATT ACGAACCCAC GCCATCCGCG ACGGGTCAAA CCATTCCGTG GAATAAGTTA 
TTCTTCCCCC AGGGATGCGG TCGCACTCCT GGCGGAGTTC CACCCCAACC GTTCAAACCA 
AAACCTAAAC CTAAACCCCC TGTAGATAGC ACCGGACAAA CTAGCAACCC CAGTCCTGGC 
GTCGCATACC CTGGTCAAGG CCCTAATGGC CCTTACGGTC CTGGCGGCTC CGGCGGCTCC 
GGCGGCCCTG GTGGTCCCGG AGGACCAAAT GGACCTTACG GACCTGGTGG TCCCGGAGGA 
CCAAATGGAC CTTACGGACC CGGCGGTACT GGCGGACCTA ATGGTCCTTA TGGCCCAGGT 
GGTCAAGATG GCTCATACAG ACCAGACGGC TCCTACGGTC CAGACGGATC TTATCAACCG 
GAAGGTTCCT ACGGACCCGA CGGATCATGG CAAGGTGGTT ACTATCCTGG CACTCCTGGA 
TCGCCTGGAA CTCCTGGGTC GCCTGGCAGC CCTGGATCCC CTGGTAGCCC GGGTACTCCT 
GGTTCTCCAG GCACACCAGG AACACCTGGT TCAGGCGGTG GATACTATCC TGGACAAGAC 
GGAACAGGGG GACCGACTGG TCCTTCAGGC GGGGGAGGTG GCTACTATCC AGGTAGTCCC 
GGCAGTCCTG GAACCCCCGG CACTCCAGGC ACACCCGGAA CACCAGGTGG CCCTGGGGGT 
CCAGGTGGAC CAGGTGGACC AGGTGGTCCA GGAGGGCCAA ACGGTCCAAA TGGGCCTGGA 
GGATCTGACG GCGGTCAATA TTATCCTGCA CTGCCCGGTA GTCCCGGTAC ACCCGGCTCC 
CCAGGCACTC CTGGTGGACC TGGTGGACCT GGTGGTCCCG GAGGCCCTGG GGGACCAGGC 
GGCCCAGGCG GCCCGGGAGG TCCAGATCAA CCAAGCTACC CTGGACAGCC AGGACAGCCT 
GGTACACCAG GATATCCAGG ACAACCAGGA TACCCAGGAC AACCCGGTAC ACCAGGACAA 
CCAGGAACAC CAGGCCAGCC AGGCTATCCA GGACAAGGTG GACAACCTAT AAATCCTGGT 
CAACCAGGAT ACCCAGGCCA GCCCGGACAA CCAGGATACC CGGGACAACC AGGTCAGCCA 
GGAACACCAG GACAACCCGG AACGCCTGGA CAACCAGGAA CGCCAGGACA ACCAGGACAA 
CCCGGCTATC CAGGAACTCC TGGGCAACCA GGGCAATCCG GATATCCAGG ACAGGGTGGA 
CCACAACAGG GAGGATACCC AGGTCAGCCA GGCCAGCCGG GATACCCAGG ACAACCAGGA 
CAGCCAGGAA CACCAGGACA GCCAGGAACA CCAGGACAGC CAGGAACACC AGGCCAGCCA 
GGAACACCAG GACAACCAGG TCAACCAGGC TACCCAGGAC AAGCTGGACA GCCTGTAAAT 
CCTGCTCAGC CGGGATACCC AGGACAGCCA GGTCAGCCAG GATACCCAGG ACAACCAGGA 
CAGCCAGGAA CTCCAGGACA GCCAGGAACA CCAGGACAGC CCGGAACACC AGGACAACCA 
GGTCAACCAG GCTACCCAGG ACAAGCTGGA CAGCCTGTAA ATCCTGCTCA GCCGGGATAC 
CCAGGTCAGC CAAGTCAGCC GGGATACCCA GGACAACCAG GACAGCCAGG AACACCAGGA 
CAGCCAGGAA CACCAGGACA ACCAGGGCAG CCCGGAACAC CAGGACAACC AGGTCAACCA 
GGCTACCCAG GACAAGGTGG ACAGCCTATA AAGCCTGGTC AGACAGGATA CCCAGGTCAG 
CCAGGCCAGC CGGGATACCC AGGACAACCA GGCCAGCCAG GAACACCAGG ACAGCCAGGA 
ACACCAGGAC AGCCAGGCCA ACCAGGCTAC CCAGGACAAG GTGGACAGCC TATAAAGCCT 
GGTCAGACAG GATACCCAGG TCAGCCAGGC CAGCCGGGAT ACCCAGGACA ACCAGGACAG 
CCAGGAACAC CAGGACAGCC AGGCCAACCG GGACAACCCG GTACACCAGG CCAGCCCGGA 
ACACCAGGAC AACCAGGTCA ACCAGGCTAC CCAGGACAAG GTGGACAGCC TGTAAATCCT 
GCTCAGCCGG GATACCCAGG ACAGCCAGGT CAGCCAGGAT ACCCAGGACA ACCAGGACAG 
CCAGGAACTC CAGGACAGCC AGGAACACCA GGACAGCCCG GAACACCAGG ACAACCAGGT 
CAACCAGGCT ACCCAGGACA AGGTGGACAG CCTGTAAATC CTGCTCAGCC GGGATACCCA 
GGTCAGCCAA GTCAGCCGGG ATACCCAGGA CAACTAGGAC AACCAGGAAC TCCAGGACAA 
CCAGGACAGC CCGGAACACC AGGACAGCCC GGAACACCAG GACAACCAGG TCAACCAGGC 
TACCCAGGAC AAGGTGGACA GCCTATAAAT CCTACTCAGC CGGGATCCCC AGGTCAGCTA 
GGTCAGCCAG GATACCCAGG ACAACCAGGA CAGCCAGGTA CTCCAGGACA ACCAGGTCAA 
CCAGGAACAC CAGGACAACC AGGAACACCA GGACAACCAG GTCAACCAGG CTACCCAGGA 
CAAGGTGGAC AGCCTATAAA GCCTGGTCAG CCAGGATACC CAGGTCAGCC AGGCCAGCCG 
GGATACCCTG GACAACCAGG ACAGCCAGGA GCACCAGGCC AACCGGGTCA GCCAGGACAA 
CCAGTGCAGC CCGGAACACC AGGACAAGCA GGTCAACCAG GCTACCCAGG ACAAGGTGGA 
CAACCTATAA AGCCTGCTCA GCCGGGACAC CCAGGCCAGC CTGGACAACC AGGGCAGCCC 
GGAACACCAG GACAACCAGG TCAACCAGGA TACCCAGGGC AACCGGGTCA GCCAGGACAA 
CCAGGGCAAC CAGGACAACA AGGCTATCCA GGACAAGGTG GACCACAACA GGGCGGTCAG 
CCTATTAATC CTAGTCAACC TGGTTACCCA GGCCAGTCAG GACAACCAGG TTACCCTGGA 
CAACCGGTTC AGCCAGGACA ACCAGGGCAG CCCGGAACAC CAGGGGAACC GGGACAGCCC 
GGTACACCAG GACAACCTGG CTATCCAGGA CAAGGTGGAC CACAACAGGG CGGTCAGCCT 
ATGAATCCTA GTCAACCCGA ATACCCAAGC CAGCCAGGAC AACCAGGACA GCCAGGAACA 
CCGGGACAAG CAGGACACCC AGGACAACCT GGACACCCAG GCGCACCAGG ACAACCAGGT 
TACCCAGGAC AACCCGGACA GACGGGCGCA CCAGGACAAG CAGGTTACCC AGGACAAGGT 
GGACCGCAAC AGGGCGGTCA GCCTATAAAT CCTAGTCAAC CAGGACATCC AGTGCAGCCA 
GGACAGCCGG GTCAACCAGG GCAGCCAGGA AAACCCGGGC AACCTGGTAC ACCAGGGCAG 
CCTGGAGCTC CAGGGCAACC TGGAACACCA GGACAACCAG GCTATCCAGG ACAAAATGGA 
CCACAACAAG GCGGACAACC CGGACAGCCA GGAACACCAG GACAACCCGG ATATCCAGGA 
CAAGGCGGAC CACAACAGGG CGGTCAGCCT ATCAATCCCG ATCAGCCCGG ACAGCCAGGT 
CCACCAGGAT ATCCAGGGCA AGGCGGACAA CCCATAAATC CTAGTCAATC AGGCTACCCG 
GGACAACCAG GACAAGGAGG CGATTCCGTC TCGTCGGGAA CTGGTGTGCA GCCACCGACA 
TATGTTCCAT CAACTCAAAT GCCACCTTTC CCAATTTATG TCATACCTTT TCCATTGCCT 
ATCGTACCAA GTCCAGGATC ATGTCCCTGC TATTTATTAA ATCCTGGAAA TAACGGTACA 
GCAACGAACG AAATGCAAGC ATCCTCACAT CCCGCAAATG GACAGAGTCA AGGATATGCT 
CCGTACGGTA TTATAGGATT CATACCTGTT GTTTTCGTTC CCTATTGTCC AGGAAATGGG 
AGTGCGATGA ACACTGCACA ACAAAACTTC CCTCAAGCAG TACCGGTGCA ATACAATTGT 
GCCCAATGTC AGGCCAATCG AGATGTCTAC AGATTCGCAG GCCGAAACAA TGGCGCCAGA 
GCGTTCAAAG AGCTTAAAGA ACTTAAATCG CTGGACGAAC TTAACGATCT CATTCGAAAT 
AGAATAAAAC CTACCACGAG GACATTACGT CAAATTGCAG CACATCCAAA AATACTTGAA 
ACAAAACACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012550714.1
CDS	158..4909
Translation	MGPGIMIKFCILAVLALSLVSADKPKTETTSTTAKKATEVPAKKTVEVPLKKASEVPVKKASEVPTKKPHVEPAREGRQLSPYPGQQDNEVVVDIQDDEKKQYYETNYDTSAYGFGYDVGPNGQFHHENRGPDGVVYGCYGYVDTEGYLRATHYVADSHGYRVVEPEKPVEVFPDEKYEYDENYEPTPSATGQTIPWNKLFFPQGCGRTPGGVPPQPFKPKPKPKPPVDSTGQTSNPSPGVAYPGQGPNGPYGPGGSGGSGGPGGPGGPNGPYGPGGPGGPNGPYGPGGTGGPNGPYGPGGQDGSYRPDGSYGPDGSYQPEGSYGPDGSWQGGYYPGTPGSPGTPGSPGSPGSPGSPGTPGSPGTPGTPGSGGGYYPGQDGTGGPTGPSGGGGGYYPGSPGSPGTPGTPGTPGTPGGPGGPGGPGGPGGPGGPNGPNGPGGSDGGQYYPALPGSPGTPGSPGTPGGPGGPGGPGGPGGPGGPGGPGGPDQPSYPGQPGQPGTPGYPGQPGYPGQPGTPGQPGTPGQPGYPGQGGQPINPGQPGYPGQPGQPGYPGQPGQPGTPGQPGTPGQPGTPGQPGQPGYPGTPGQPGQSGYPGQGGPQQGGYPGQPGQPGYPGQPGQPGTPGQPGTPGQPGTPGQPGTPGQPGQPGYPGQAGQPVNPAQPGYPGQPGQPGYPGQPGQPGTPGQPGTPGQPGTPGQPGQPGYPGQAGQPVNPAQPGYPGQPSQPGYPGQPGQPGTPGQPGTPGQPGQPGTPGQPGQPGYPGQGGQPIKPGQTGYPGQPGQPGYPGQPGQPGTPGQPGTPGQPGQPGYPGQGGQPIKPGQTGYPGQPGQPGYPGQPGQPGTPGQPGQPGQPGTPGQPGTPGQPGQPGYPGQGGQPVNPAQPGYPGQPGQPGYPGQPGQPGTPGQPGTPGQPGTPGQPGQPGYPGQGGQPVNPAQPGYPGQPSQPGYPGQLGQPGTPGQPGQPGTPGQPGTPGQPGQPGYPGQGGQPINPTQPGSPGQLGQPGYPGQPGQPGTPGQPGQPGTPGQPGTPGQPGQPGYPGQGGQPIKPGQPGYPGQPGQPGYPGQPGQPGAPGQPGQPGQPVQPGTPGQAGQPGYPGQGGQPIKPAQPGHPGQPGQPGQPGTPGQPGQPGYPGQPGQPGQPGQPGQQGYPGQGGPQQGGQPINPSQPGYPGQSGQPGYPGQPVQPGQPGQPGTPGEPGQPGTPGQPGYPGQGGPQQGGQPMNPSQPEYPSQPGQPGQPGTPGQAGHPGQPGHPGAPGQPGYPGQPGQTGAPGQAGYPGQGGPQQGGQPINPSQPGHPVQPGQPGQPGQPGKPGQPGTPGQPGAPGQPGTPGQPGYPGQNGPQQGGQPGQPGTPGQPGYPGQGGPQQGGQPINPDQPGQPGPPGYPGQGGQPINPSQSGYPGQPGQGGDSVSSGTGVQPPTYVPSTQMPPFPIYVIPFPLPIVPSPGSCPCYLLNPGNNGTATNEMQASSHPANGQSQGYAPYGIIGFIPVVFVPYCPGNGSAMNTAQQNFPQAVPVQYNCAQCQANRDVYRFAGRNNGARAFKELKELKSLDELNDLIRNRIKPTTRTLRQIAAHPKILETKH

Target ORF information:

RefSeq Version	XM_012695260.1
Organism	Bombyx mori(Domestic silkworm)
Definition	Bombyx mori collagen (CPR146), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012695260.1

ORF Insert Sequence:

ATGGGACCCG GAATAATGAT TAAATTTTGT ATCTTGGCGG TGCTAGCATT GTCACTGGTT 
AGCGCCGATA AGCCAAAAAC TGAGACAACT TCAACGACCG CGAAAAAGGC AACTGAAGTA 
CCCGCGAAGA AGACAGTCGA AGTACCTTTG AAGAAGGCAT CTGAAGTGCC CGTGAAGAAG 
GCATCAGAAG TGCCAACAAA GAAGCCCCAT GTAGAACCCG CGCGTGAAGG AAGGCAGTTG 
AGTCCTTACC CTGGACAACA AGACAACGAA GTGGTGGTCG ACATTCAGGA CGACGAAAAG 
AAGCAGTACT ACGAAACCAA CTATGACACA AGTGCGTATG GTTTCGGATA CGATGTCGGC 
CCAAACGGAC AGTTTCATCA CGAGAACCGC GGTCCCGATG GTGTCGTCTA CGGATGTTAT 
GGTTACGTCG ACACCGAGGG CTACCTCCGC GCCACCCACT ACGTTGCCGA CAGCCACGGC 
TACCGAGTCG TAGAACCAGA AAAACCTGTC GAAGTTTTCC CCGATGAGAA ATACGAATAT 
GACGAAAATT ACGAACCCAC GCCATCCGCG ACGGGTCAAA CCATTCCGTG GAATAAGTTA 
TTCTTCCCCC AGGGATGCGG TCGCACTCCT GGCGGAGTTC CACCCCAACC GTTCAAACCA 
AAACCTAAAC CTAAACCCCC TGTAGATAGC ACCGGACAAA CTAGCAACCC CAGTCCTGGC 
GTCGCATACC CTGGTCAAGG CCCTAATGGC CCTTACGGTC CTGGCGGCTC CGGCGGCTCC 
GGCGGCCCTG GTGGTCCCGG AGGACCAAAT GGACCTTACG GACCTGGTGG TCCCGGAGGA 
CCAAATGGAC CTTACGGACC CGGCGGTACT GGCGGACCTA ATGGTCCTTA TGGCCCAGGT 
GGTCAAGATG GCTCATACAG ACCAGACGGC TCCTACGGTC CAGACGGATC TTATCAACCG 
GAAGGTTCCT ACGGACCCGA CGGATCATGG CAAGGTGGTT ACTATCCTGG CACTCCTGGA 
TCGCCTGGAA CTCCTGGGTC GCCTGGCAGC CCTGGATCCC CTGGTAGCCC GGGTACTCCT 
GGTTCTCCAG GCACACCAGG AACACCTGGT TCAGGCGGTG GATACTATCC TGGACAAGAC 
GGAACAGGGG GACCGACTGG TCCTTCAGGC GGGGGAGGTG GCTACTATCC AGGTAGTCCC 
GGCAGTCCTG GAACCCCCGG CACTCCAGGC ACACCCGGAA CACCAGGTGG CCCTGGGGGT 
CCAGGTGGAC CAGGTGGACC AGGTGGTCCA GGAGGGCCAA ACGGTCCAAA TGGGCCTGGA 
GGATCTGACG GCGGTCAATA TTATCCTGCA CTGCCCGGTA GTCCCGGTAC ACCCGGCTCC 
CCAGGCACTC CTGGTGGACC TGGTGGACCT GGTGGTCCCG GAGGCCCTGG GGGACCAGGC 
GGCCCAGGCG GCCCGGGAGG TCCAGATCAA CCAAGCTACC CTGGACAGCC AGGACAGCCT 
GGTACACCAG GATATCCAGG ACAACCAGGA TACCCAGGAC AACCCGGTAC ACCAGGACAA 
CCAGGAACAC CAGGCCAGCC AGGCTATCCA GGACAAGGTG GACAACCTAT AAATCCTGGT 
CAACCAGGAT ACCCAGGCCA GCCCGGACAA CCAGGATACC CGGGACAACC AGGTCAGCCA 
GGAACACCAG GACAACCCGG AACGCCTGGA CAACCAGGAA CGCCAGGACA ACCAGGACAA 
CCCGGCTATC CAGGAACTCC TGGGCAACCA GGGCAATCCG GATATCCAGG ACAGGGTGGA 
CCACAACAGG GAGGATACCC AGGTCAGCCA GGCCAGCCGG GATACCCAGG ACAACCAGGA 
CAGCCAGGAA CACCAGGACA GCCAGGAACA CCAGGACAGC CAGGAACACC AGGCCAGCCA 
GGAACACCAG GACAACCAGG TCAACCAGGC TACCCAGGAC AAGCTGGACA GCCTGTAAAT 
CCTGCTCAGC CGGGATACCC AGGACAGCCA GGTCAGCCAG GATACCCAGG ACAACCAGGA 
CAGCCAGGAA CTCCAGGACA GCCAGGAACA CCAGGACAGC CCGGAACACC AGGACAACCA 
GGTCAACCAG GCTACCCAGG ACAAGCTGGA CAGCCTGTAA ATCCTGCTCA GCCGGGATAC 
CCAGGTCAGC CAAGTCAGCC GGGATACCCA GGACAACCAG GACAGCCAGG AACACCAGGA 
CAGCCAGGAA CACCAGGACA ACCAGGGCAG CCCGGAACAC CAGGACAACC AGGTCAACCA 
GGCTACCCAG GACAAGGTGG ACAGCCTATA AAGCCTGGTC AGACAGGATA CCCAGGTCAG 
CCAGGCCAGC CGGGATACCC AGGACAACCA GGCCAGCCAG GAACACCAGG ACAGCCAGGA 
ACACCAGGAC AGCCAGGCCA ACCAGGCTAC CCAGGACAAG GTGGACAGCC TATAAAGCCT 
GGTCAGACAG GATACCCAGG TCAGCCAGGC CAGCCGGGAT ACCCAGGACA ACCAGGACAG 
CCAGGAACAC CAGGACAGCC AGGCCAACCG GGACAACCCG GTACACCAGG CCAGCCCGGA 
ACACCAGGAC AACCAGGTCA ACCAGGCTAC CCAGGACAAG GTGGACAGCC TGTAAATCCT 
GCTCAGCCGG GATACCCAGG ACAGCCAGGT CAGCCAGGAT ACCCAGGACA ACCAGGACAG 
CCAGGAACTC CAGGACAGCC AGGAACACCA GGACAGCCCG GAACACCAGG ACAACCAGGT 
CAACCAGGCT ACCCAGGACA AGGTGGACAG CCTGTAAATC CTGCTCAGCC GGGATACCCA 
GGTCAGCCAA GTCAGCCGGG ATACCCAGGA CAACTAGGAC AACCAGGAAC TCCAGGACAA 
CCAGGACAGC CCGGAACACC AGGACAGCCC GGAACACCAG GACAACCAGG TCAACCAGGC 
TACCCAGGAC AAGGTGGACA GCCTATAAAT CCTACTCAGC CGGGATCCCC AGGTCAGCTA 
GGTCAGCCAG GATACCCAGG ACAACCAGGA CAGCCAGGTA CTCCAGGACA ACCAGGTCAA 
CCAGGAACAC CAGGACAACC AGGAACACCA GGACAACCAG GTCAACCAGG CTACCCAGGA 
CAAGGTGGAC AGCCTATAAA GCCTGGTCAG CCAGGATACC CAGGTCAGCC AGGCCAGCCG 
GGATACCCTG GACAACCAGG ACAGCCAGGA GCACCAGGCC AACCGGGTCA GCCAGGACAA 
CCAGTGCAGC CCGGAACACC AGGACAAGCA GGTCAACCAG GCTACCCAGG ACAAGGTGGA 
CAACCTATAA AGCCTGCTCA GCCGGGACAC CCAGGCCAGC CTGGACAACC AGGGCAGCCC 
GGAACACCAG GACAACCAGG TCAACCAGGA TACCCAGGGC AACCGGGTCA GCCAGGACAA 
CCAGGGCAAC CAGGACAACA AGGCTATCCA GGACAAGGTG GACCACAACA GGGCGGTCAG 
CCTATTAATC CTAGTCAACC TGGTTACCCA GGCCAGTCAG GACAACCAGG TTACCCTGGA 
CAACCGGTTC AGCCAGGACA ACCAGGGCAG CCCGGAACAC CAGGGGAACC GGGACAGCCC 
GGTACACCAG GACAACCTGG CTATCCAGGA CAAGGTGGAC CACAACAGGG CGGTCAGCCT 
ATGAATCCTA GTCAACCCGA ATACCCAAGC CAGCCAGGAC AACCAGGACA GCCAGGAACA 
CCGGGACAAG CAGGACACCC AGGACAACCT GGACACCCAG GCGCACCAGG ACAACCAGGT 
TACCCAGGAC AACCCGGACA GACGGGCGCA CCAGGACAAG CAGGTTACCC AGGACAAGGT 
GGACCGCAAC AGGGCGGTCA GCCTATAAAT CCTAGTCAAC CAGGACATCC AGTGCAGCCA 
GGACAGCCGG GTCAACCAGG GCAGCCAGGA AAACCCGGGC AACCTGGTAC ACCAGGGCAG 
CCTGGAGCTC CAGGGCAACC TGGAACACCA GGACAACCAG GCTATCCAGG ACAAAATGGA 
CCACAACAAG GCGGACAACC CGGACAGCCA GGAACACCAG GACAACCCGG ATATCCAGGA 
CAAGGCGGAC CACAACAGGG CGGTCAGCCT ATCAATCCCG ATCAGCCCGG ACAGCCAGGT 
CCACCAGGAT ATCCAGGGCA AGGCGGACAA CCCATAAATC CTAGTCAATC AGGCTACCCG 
GGACAACCAG GACAAGGAGG CGATTCCGTC TCGTCGGGAA CTGGTGTGCA GCCACCGACA 
TATGTTCCAT CAACTCAAAT GCCACCTTTC CCAATTTATG TCATACCTTT TCCATTGCCT 
ATCGTACCAA GTCCAGGATC ATGTCCCTGC TATTTATTAA ATCCTGGAAA TAACGGTACA 
GCAACGAACG AAATGCAAGC ATCCTCACAT CCCGCAAATG GACAGAGTCA AGGATATGCT 
CCGTACGGTA TTATAGGATT CATACCTGTT GTTTTCGTTC CCTATTGTCC AGGAAATGGG 
AGTGCGATGA ACACTGCACA ACAAAACTTC CCTCAAGCAG TACCGGTGCA ATACAATTGT 
GCCCAATGTC AGGCCAATCG AGATGTCTAC AGATTCGCAG GCCGAAACAA TGGCGCCAGA 
GCGTTCAAAG AGCTTAAAGA ACTTAAATCG CTGGACGAAC TTAACGATCT CATTCGAAAT 
AGAATAAAAC CTACCACGAG GACATTACGT CAAATTGCAG CACATCCAAA AATACTTGAA 
ACAAAACACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
BMC genomics15820(2014 Sep)
Ou J,Deng HM,Zheng SC,Huang LH,Feng QL,Liu L

Genome-wide identification of cuticular protein genes in the silkworm, Bombyx mori.
Insect biochemistry and molecular biology38(12)1138-46(2008 Dec)
Futahashi R,Okamoto S,Kawasaki H,Zhong YS,Iwanaga M,Mita K,Fujiwara H

Identification of a Bombyx collagenous protein with multiple short domains of Gly-Xaa-Yaa repeats: cDNA characterization and regulation of expression.
Insect biochemistry and molecular biology26(7)677-85(1996 Jul)
Chareyre P,Besson MT,Fourche J,Bosquet G

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CPR146 cDNA ORF clone, Bombyx mori(domestic silkworm)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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