Fib-l cDNA ORF clone, Bombyx mori(domestic silkworm)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fib-l
Entrez Gene ID	693047
Full Name	silk fibroin light chain
Synonyms	FIBL,fibroin
Gene Type	protein-coding
Organism	Bombyx mori(domestic silkworm)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001044023.1	NP_001037488.1	fibroin light chain precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
Ou J, Deng HM, Zheng SC, Huang LH, Feng QL, Liu L
BMC genomics15820(2014 Sep)

Large scale full-length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori.
Suetsugu Y, Futahashi R, Kanamori H, Kadono-Okuda K, Sasanuma S, Narukawa J, Ajimura M, Jouraku A, Namiki N, Shimomura M, Sezutsu H, Osanai-Futahashi M, Suzuki MG, Daimon T, Shinoda T, Taniai K, Asaoka K, Niwa R, Kawaoka S, Katsuma S, Tamura T, Noda H, Kasahara M, Sugano S, Suzuki Y, Fujiwara H, Kataoka H, Arunkumar KP, Tomar A, Nagaraju J, Goldsmith MR, Feng Q, Xia Q, Yamamoto K, Shimada T, Mita K
G3 (Bethesda, Md.)3(9)1481-92(2013 Sep)

Identification of conserved microRNAs in Bombyx mori (silkworm) and regulation of fibroin L chain production by microRNAs in heterologous system.
Cao J, Tong C, Wu X, Lv J, Yang Z, Jin Y
Insect biochemistry and molecular biology38(12)1066-71(2008 Dec)

Time-resolved structural investigation of regenerated silk fibroin nanofibers treated with solvent vapor.
Jeong L, Lee KY, Liu JW, Park WH
International journal of biological macromolecules38(2)140-4(2006 Mar)

A draft sequence for the genome of the domesticated silkworm (Bombyx mori).
Xia Q, Zhou Z, Lu C, Cheng D, Dai F, Li B, Zhao P, Zha X, Cheng T, Chai C, Pan G, Xu J, Liu C, Lin Y, Qian J, Hou Y, Wu Z, Li G, Pan M, Li C, Shen Y, Lan X, Yuan L, Li T, Xu H, Yang G, Wan Y, Zhu Y, Yu M, Shen W, Wu D, Xiang Z, Yu J, Wang J, Li R, Shi J, Li H, Li G, Su J, Wang X, Li G, Zhang Z, Wu Q, Li J, Zhang Q, Wei N, Xu J, Sun H, Dong L, Liu D, Zhao S, Zhao X, Meng Q, Lan F, Huang X, Li Y, Fang L, Li C, Li D, Sun Y, Zhang Z, Yang Z, Huang Y, Xi Y, Qi Q, He D, Huang H, Zhang X, Wang Z, Li W, Cao Y, Yu Y, Yu H, Li J, Ye J, Chen H, Zhou Y, Liu B, Wang J, Ye J, Ji H, Li S, Ni P, Zhang J, Zhang Y, Zheng H, Mao B, Wang W, Ye C, Li S, Wang J, Wong GK, Yang H,
Science (New York, N.Y.)306(5703)1937-40(2004 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Fib-l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fib-l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBg00056	NM_001044023.1 Latest version!	Bombyx mori silk fibroin light chain (Fib-l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fib-l ( NM_001044023.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> NP_001037488.1 Bombyx mori silk fibroin light chain (Fib-l), mRNA.

Price & Availability↑

CloneID	OBg00056
Clone ID Related Accession (Same CDS sequence)	NM_001044023.1
Accession Version	NM_001044023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Bombyx mori(domestic silkworm)
Product	fibroin light chain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M76430.1. On Sep 6, 2006 this sequence version replaced NM_001044024.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03083555, SAMN03084334 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCCTA TATTTTTGGT ATTACTCGTC GCTACAAGCG CCTACGCTGC ACCATCGGTG 
ACCATCAATC AATACAGTGA TAATGAAATT CCACGCGACA TTGATGATGG AAAAGCTAGT 
TCCGTAATCT CACGTCGATG GGACTACGTC GATGACACTG ACAAAAGCAT CGCCATCCTC 
AACGTTCAAG AGATCTTGAA GGACATGGCC AGCCAGGGCG ATTATGCAAG TCAAGCATCA 
GCGGTGGCCC AAACCGCCGG AATTATCGCC CATCTATCTG CCGGTATCCC CGGTGATGCC 
TGTGCAGCCG CTAACGTCAT TAACTCTTAC ACAGACGGCG TCAGGTCCGG AAACTTCGCC 
GGCTTCAGAC AATCTCTCGG TCCCTTCTTC GGACACGTGG GACAAAACTT GAATCTTATC 
AATCAACTCG TCATCAACCC TGGTCAACTC CGATACTCTG TCGGACCAGC CCTGGGTTGT 
GCCGGAGGTG GAAGAATCTA TGACTTCGAA GCCGCTTGGG ATGCAATCTT AGCCAGCAGT 
GACTCTAGTT TCTTAAATGA AGAGTACTGC ATCGTCAAGA GATTGTACAA CTCTCGCAAC 
AGCCAAAGCA ACAACATCGC TGCCTACATA ACCGCTCACT TACTTCCTCC GGTTGCTCAA 
GTGTTCCACC AATCAGCTGG ATCAATCACA GACCTCCTGA GAGGCGTTGG CAACGGTAAT 
GACGCGACCG GCTTAGTTGC TAATGCTCAA AGATATATTG CACAAGCAGC CAGCCAGGTT 
CACGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001037488.1
CDS	42..830
Translation	MKPIFLVLLVATSAYAAPSVTINQYSDNEIPRDIDDGKASSVISRRWDYVDDTDKSIAILNVQEILKDMASQGDYASQASAVAQTAGIIAHLSAGIPGDACAAANVINSYTDGVRSGNFAGFRQSLGPFFGHVGQNLNLINQLVINPGQLRYSVGPALGCAGGGRIYDFEAAWDAILASSDSSFLNEEYCIVKRLYNSRNSQSNNIAAYITAHLLPPVAQVFHQSAGSITDLLRGVGNGNDATGLVANAQRYIAQAASQVHV

Target ORF information:

RefSeq Version	NM_001044023.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori silk fibroin light chain (Fib-l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044023.1

ORF Insert Sequence:

ATGAAGCCTA TATTTTTGGT ATTACTCGTC GCTACAAGCG CCTACGCTGC ACCATCGGTG 
ACCATCAATC AATACAGTGA TAATGAAATT CCACGCGACA TTGATGATGG AAAAGCTAGT 
TCCGTAATCT CACGTCGATG GGACTACGTC GATGACACTG ACAAAAGCAT CGCCATCCTC 
AACGTTCAAG AGATCTTGAA GGACATGGCC AGCCAGGGCG ATTATGCAAG TCAAGCATCA 
GCGGTGGCCC AAACCGCCGG AATTATCGCC CATCTATCTG CCGGTATCCC CGGTGATGCC 
TGTGCAGCCG CTAACGTCAT TAACTCTTAC ACAGACGGCG TCAGGTCCGG AAACTTCGCC 
GGCTTCAGAC AATCTCTCGG TCCCTTCTTC GGACACGTGG GACAAAACTT GAATCTTATC 
AATCAACTCG TCATCAACCC TGGTCAACTC CGATACTCTG TCGGACCAGC CCTGGGTTGT 
GCCGGAGGTG GAAGAATCTA TGACTTCGAA GCCGCTTGGG ATGCAATCTT AGCCAGCAGT 
GACTCTAGTT TCTTAAATGA AGAGTACTGC ATCGTCAAGA GATTGTACAA CTCTCGCAAC 
AGCCAAAGCA ACAACATCGC TGCCTACATA ACCGCTCACT TACTTCCTCC GGTTGCTCAA 
GTGTTCCACC AATCAGCTGG ATCAATCACA GACCTCCTGA GAGGCGTTGG CAACGGTAAT 
GACGCGACCG GCTTAGTTGC TAATGCTCAA AGATATATTG CACAAGCAGC CAGCCAGGTT 
CACGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
BMC genomics15820(2014 Sep)
Ou J,Deng HM,Zheng SC,Huang LH,Feng QL,Liu L

Large scale full-length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori.
G3 (Bethesda, Md.)3(9)1481-92(2013 Sep)
Suetsugu Y,Futahashi R,Kanamori H,Kadono-Okuda K,Sasanuma S,Narukawa J,Ajimura M,Jouraku A,Namiki N,Shimomura M,Sezutsu H,Osanai-Futahashi M,Suzuki MG,Daimon T,Shinoda T,Taniai K,Asaoka K,Niwa R,Kawaoka S,Katsuma S,Tamura T,Noda H,Kasahara M,Sugano S,Suzuki Y,Fujiwara H,Kataoka H,Arunkumar KP,Tomar A,Nagaraju J,Goldsmith MR,Feng Q,Xia Q,Yamamoto K,Shimada T,Mita K

Identification of conserved microRNAs in Bombyx mori (silkworm) and regulation of fibroin L chain production by microRNAs in heterologous system.
Insect biochemistry and molecular biology38(12)1066-71(2008 Dec)
Cao J,Tong C,Wu X,Lv J,Yang Z,Jin Y

Time-resolved structural investigation of regenerated silk fibroin nanofibers treated with solvent vapor.
International journal of biological macromolecules38(2)140-4(2006 Mar)
Jeong L,Lee KY,Liu JW,Park WH

A draft sequence for the genome of the domesticated silkworm (Bombyx mori).
Science (New York, N.Y.)306(5703)1937-40(2004 Dec)
Xia Q,Zhou Z,Lu C,Cheng D,Dai F,Li B,Zhao P,Zha X,Cheng T,Chai C,Pan G,Xu J,Liu C,Lin Y,Qian J,Hou Y,Wu Z,Li G,Pan M,Li C,Shen Y,Lan X,Yuan L,Li T,Xu H,Yang G,Wan Y,Zhu Y,Yu M,Shen W,Wu D,Xiang Z,Yu J,Wang J,Li R,Shi J,Li H,Li G,Su J,Wang X,Li G,Zhang Z,Wu Q,Li J,Zhang Q,Wei N,Xu J,Sun H,Dong L,Liu D,Zhao S,Zhao X,Meng Q,Lan F,Huang X,Li Y,Fang L,Li C,Li D,Sun Y,Zhang Z,Yang Z,Huang Y,Xi Y,Qi Q,He D,Huang H,Zhang X,Wang Z,Li W,Cao Y,Yu Y,Yu H,Li J,Ye J,Chen H,Zhou Y,Liu B,Wang J,Ye J,Ji H,Li S,Ni P,Zhang J,Zhang Y,Zheng H,Mao B,Wang W,Ye C,Li S,Wang J,Wong GK,Yang H,

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Fib-l cDNA ORF clone, Bombyx mori(domestic silkworm)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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