SGF3 cDNA ORF clone, Bombyx mori(domestic silkworm)

Gene
Clones
gRNAs

Summary

Gene Symbol	SGF3
Entrez Gene ID	693025
Full Name	POU domain protein
Synonyms	Pou-m1,Pou-m2
Gene Type	protein-coding
Organism	Bombyx mori(domestic silkworm)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001043991.2	NP_001037456.2	silk gland factor 3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

BmILF and i-motif structure are involved in transcriptional regulation of BmPOUM2 in Bombyx mori.
Niu K, Zhang X, Deng H, Wu F, Ren Y, Xiang H, Zheng S, Liu L, Huang L, Zeng B, Li S, Xia Q, Song Q, Palli SR, Feng Q
Nucleic acids research46(4)1710-1723(2018 02)

The POU homeodomain transcription factor POUM2 and broad complex isoform 2 transcription factor induced by 20-hydroxyecdysone collaboratively regulate vitellogenin gene expression and egg formation in the silkworm Bombyx mori.
Lin Y, Liu H, Yang C, Gu J, Shen G, Zhang H, Chen E, Han C, Zhang Y, Xu Y, Wu J, Xia Q
Insect molecular biology26(5)496-506(2017 10)

Identification of the binding domains and key amino acids for the interaction of the transcription factors BmPOUM2 and BmAbd-A in Bombyx mori.
He Y, Deng H, Hu Q, Zhu Z, Liu L, Zheng S, Song Q, Feng Q
Insect biochemistry and molecular biology8141-50(2017 02)

Biochemical characterization and functional analysis of the POU transcription factor POU-M2 of Bombyx mori.
Liu L, Li Y, Wang Y, Zhao P, Wei S, Li Z, Chang H, He H
International journal of biological macromolecules86701-8(2016 May)

The homeodomain transcription factors antennapedia and POU-M2 regulate the transcription of the steroidogenic enzyme gene Phantom in the silkworm.
Meng M, Cheng DJ, Peng J, Qian WL, Li JR, Dai DD, Zhang TL, Xia QY
The Journal of biological chemistry290(40)24438-52(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SGF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SGF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBg00042	NM_001043991.2 Latest version!	Bombyx mori POU domain protein (SGF3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SGF3 ( NM_001043991.2 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> NP_001037456.2 Bombyx mori POU domain protein (SGF3), mRNA.

Price & Availability↑

CloneID	OBg00042
Clone ID Related Accession (Same CDS sequence)	NM_001043991.2
Accession Version	NM_001043991.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1563724800000
Organism	Bombyx mori(domestic silkworm)
Product	silk gland factor 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY334012.1. On Jan 3, 2008 this sequence version replaced NM_001043991.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY334012.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCGGCGA CCACGTACAT GCCCGCCGAG ATGGAGCTCG GCAACATCGG CGGGTACCAC 
GCGGCGTCGC CGCGTAGTGC TGAGCCTGCC GACATGAAGT ACCAGCATCC GTTGCACTCG 
GGCGGGTCGC CGTCCCCGGG GGCGCCCGTG ATAGGGAACC CTTGGACGTC ACTGCCACCC 
GCCGACCCCT GGGCGATGCA CCAGCATCAC GCACACGCAC ATCAACCAGA CGTGAAGCCT 
CCCCCGGCTC CTCACGACCA CCGCCACTTG CAGCATGCGG CCCACGGCTG GCACGCGCCC 
GTCGTCAGCC CGCATTACGG TGCGGGCTCG CCCGTCACAC TGCATGGAGG ATACCCAATG 
CCCGTGCACC AACACCATAT GCTCAGAGAC ATCCAGCCCT CGCCGCATCC GCTGCATCAT 
CACGCCATGG AGCGGGATCA GCCCGAGGAG GACACCCCCA CGAGCGACGA CCTCGAAGCA 
TTCGCCAAAC AGTTCAAGCA GCGCCGCATC AAGCTCGGTT TCACGCAGGC GGACGTCGGG 
CTCGCGCTCG GCACGCTCTA CGGGAATGTG TTTTCACAGA CGACTATCTG TCGTTTCGAA 
GCGTTACAGC TCAGTTTTAA AAATATGTGT AAATTAAAGC CGCTGCTGCA GAAGTGGCTC 
GAGGAGGCGG ACTCGACGAC GGGCAGCCCG ACCAGCATCG ACAAAATAGC GGCGCAGGGT 
CGGAAGAGGA AGAAGCGCAC CTCTATAGAG GTTTCGGTGA AAGGTGCGCT AGAGCAACAC 
TTCCACAAGC AGCCGAAACC GTCCGCTCAA GAAATCACGT CGTTAGCGGA CAGCCTGCAG 
CTGGAGAAAG AGGTGGTGCG CGTGTGGTTC TGCAACAGGA GACAGAAAGA GAAGAGGATG 
ACGCCACCGA ACACGCTCGG CGGCGAGATG ATGGAGGGCA TGGGGCACGC ACACTACGGA 
CACGGAGACG TGCACGGGTC CCCGCTGCAG CACTCCCCGC CGGGCCTGTC CCCGCAGCAC 
GGGCTGCCGC AGGGCGCCCA CACACTGGCA GCGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001037456.2
CDS	1..1059
Translation	MAATTYMPAEMELGNIGGYHAASPRSAEPADMKYQHPLHSGGSPSPGAPVIGNPWTSLPPADPWAMHQHHAHAHQPDVKPPPAPHDHRHLQHAAHGWHAPVVSPHYGAGSPVTLHGGYPMPVHQHHMLRDIQPSPHPLHHHAMERDQPEEDTPTSDDLEAFAKQFKQRRIKLGFTQADVGLALGTLYGNVFSQTTICRFEALQLSFKNMCKLKPLLQKWLEEADSTTGSPTSIDKIAAQGRKRKKRTSIEVSVKGALEQHFHKQPKPSAQEITSLADSLQLEKEVVRVWFCNRRQKEKRMTPPNTLGGEMMEGMGHAHYGHGDVHGSPLQHSPPGLSPQHGLPQGAHTLAAH

Target ORF information:

RefSeq Version	NM_001043991.2
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori POU domain protein (SGF3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043991.2

ORF Insert Sequence:

ATGGCGGCGA CCACGTACAT GCCCGCCGAG ATGGAGCTCG GCAACATCGG CGGGTACCAC 
GCGGCGTCGC CGCGTAGTGC TGAGCCTGCC GACATGAAGT ACCAGCATCC GTTGCACTCG 
GGCGGGTCGC CGTCCCCGGG GGCGCCCGTG ATAGGGAACC CTTGGACGTC ACTGCCACCC 
GCCGACCCCT GGGCGATGCA CCAGCATCAC GCACACGCAC ATCAACCAGA CGTGAAGCCT 
CCCCCGGCTC CTCACGACCA CCGCCACTTG CAGCATGCGG CCCACGGCTG GCACGCGCCC 
GTCGTCAGCC CGCATTACGG TGCGGGCTCG CCCGTCACAC TGCATGGAGG ATACCCAATG 
CCCGTGCACC AACACCATAT GCTCAGAGAC ATCCAGCCCT CGCCGCATCC GCTGCATCAT 
CACGCCATGG AGCGGGATCA GCCCGAGGAG GACACCCCCA CGAGCGACGA CCTCGAAGCA 
TTCGCCAAAC AGTTCAAGCA GCGCCGCATC AAGCTCGGTT TCACGCAGGC GGACGTCGGG 
CTCGCGCTCG GCACGCTCTA CGGGAATGTG TTTTCACAGA CGACTATCTG TCGTTTCGAA 
GCGTTACAGC TCAGTTTTAA AAATATGTGT AAATTAAAGC CGCTGCTGCA GAAGTGGCTC 
GAGGAGGCGG ACTCGACGAC GGGCAGCCCG ACCAGCATCG ACAAAATAGC GGCGCAGGGT 
CGGAAGAGGA AGAAGCGCAC CTCTATAGAG GTTTCGGTGA AAGGTGCGCT AGAGCAACAC 
TTCCACAAGC AGCCGAAACC GTCCGCTCAA GAAATCACGT CGTTAGCGGA CAGCCTGCAG 
CTGGAGAAAG AGGTGGTGCG CGTGTGGTTC TGCAACAGGA GACAGAAAGA GAAGAGGATG 
ACGCCACCGA ACACGCTCGG CGGCGAGATG ATGGAGGGCA TGGGGCACGC ACACTACGGA 
CACGGAGACG TGCACGGGTC CCCGCTGCAG CACTCCCCGC CGGGCCTGTC CCCGCAGCAC 
GGGCTGCCGC AGGGCGCCCA CACACTGGCA GCGCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

BmILF and i-motif structure are involved in transcriptional regulation of BmPOUM2 in Bombyx mori.
Nucleic acids research46(4)1710-1723(2018 02)
Niu K,Zhang X,Deng H,Wu F,Ren Y,Xiang H,Zheng S,Liu L,Huang L,Zeng B,Li S,Xia Q,Song Q,Palli SR,Feng Q

The POU homeodomain transcription factor POUM2 and broad complex isoform 2 transcription factor induced by 20-hydroxyecdysone collaboratively regulate vitellogenin gene expression and egg formation in the silkworm Bombyx mori.
Insect molecular biology26(5)496-506(2017 10)
Lin Y,Liu H,Yang C,Gu J,Shen G,Zhang H,Chen E,Han C,Zhang Y,Xu Y,Wu J,Xia Q

Identification of the binding domains and key amino acids for the interaction of the transcription factors BmPOUM2 and BmAbd-A in Bombyx mori.
Insect biochemistry and molecular biology8141-50(2017 02)
He Y,Deng H,Hu Q,Zhu Z,Liu L,Zheng S,Song Q,Feng Q

Biochemical characterization and functional analysis of the POU transcription factor POU-M2 of Bombyx mori.
International journal of biological macromolecules86701-8(2016 May)
Liu L,Li Y,Wang Y,Zhao P,Wei S,Li Z,Chang H,He H

The homeodomain transcription factors antennapedia and POU-M2 regulate the transcription of the steroidogenic enzyme gene Phantom in the silkworm.
The Journal of biological chemistry290(40)24438-52(2015 Oct)
Meng M,Cheng DJ,Peng J,Qian WL,Li JR,Dai DD,Zhang TL,Xia QY