E74 cDNA ORF clone, Bombyx mori(domestic silkworm)

Gene
Clones
gRNAs

Summary

Gene Symbol	E74
Entrez Gene ID	693011
Full Name	transcription factor E74
Synonyms	E74B
Gene Type	protein-coding
Organism	Bombyx mori(domestic silkworm)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001043979.1	NP_001037444.1	transcription factor E74

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Transcription factor E74A affects the ecdysone titer by regulating the expression of the EO gene in the silkworm, Bomby mori.
Sun W, Wang CF, Zhang Z
Biochimica et biophysica acta. General subjects1861(3)551-558(2017 Mar)

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
Ou J, Deng HM, Zheng SC, Huang LH, Feng QL, Liu L
BMC genomics15820(2014 Sep)

Stage-specific activation of the E74B promoter by low ecdysone concentrations in the wing disc of Bombyx mori.
Wang HB, Iwanaga M, Kawasaki H
Gene537(2)322-7(2014 Mar)

Ecdysone-responsive transcription factors determine the expression region of target cuticular protein genes in the epidermis of Bombyx mori.
Ali MS, Iwanaga M, Kawasaki H
Development genes and evolution222(2)89-97(2012 Apr)

20-Hydroxyecdysone regulation of two isoforms of the Ets transcription factor E74 gene in programmed cell death in the silkworm anterior silk gland.
Sekimoto T, Iwami M, Sakurai S
Insect molecular biology16(5)581-90(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following E74 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E74 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBg13604	NM_001043979.1 Latest version!	Bombyx mori transcription factor E74 (E74), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

E74 ( NM_001043979.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> NP_001037444.1 Bombyx mori transcription factor E74 (E74), mRNA.

Price & Availability↑

CloneID	OBg13604
Clone ID Related Accession (Same CDS sequence)	NM_001043979.1
Accession Version	NM_001043979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556812800000
Organism	Bombyx mori(domestic silkworm)
Product	transcription factor E74
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ471939.1. On Sep 6, 2006 this sequence version replaced NM_001043980.1. ##Evidence-Data-START## Transcript exon combination :: DQ471939.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00016912, SAMD00016913 [ECO:0000350] ##Evidence-Data-END##

ATGCCATTTA TTGAAGACGA GTGGTGGTCC GCCGAGAATG AGGGCAGAAT GGTCGATCTC 
TCAAATTGCC TTCAGGGACA GTTTCAGGAC GCGGTAGTGG CTGCCGGCGG TCAGGCGGCC 
GCCCAGCTTC AGCAGATGGC CTCGTCACTA GGCGAGCTGT CGCAGGCCGA GCTGTCCAAC 
ATCGTGGGCG GCCTCACCCT GGAGCCGGAG AGCTCTGAAG CGGCAGACCC CGACGACATC 
CTGAAGCAGC TCGGGGAGAC CGCGTTCGAC AACTTCGATA CGTTTTTCAC GGATCTCACT 
AACGCCACCG CTTCGGGCGC ACCACCTATC GAGATAAAGC AAGAAGAGAA CAACAACATA 
TCGTCGCCAG CGTCCAGCAG TCAACTGCAA GGGTACTACC CTCAAAGTCA ATTACATTTA 
CAAAACAACG GGCAGCAAAG ATTGCAGCAA CTCCTTAGAT CGGGCACCAA CGCCATTAAC 
AATGCCGTTA ATAATAACAT TAACAATGGA AGATATAATA TTGCGTCGCA AAATCCGTTG 
CTCGCCGAGA AATTATCTTC AACGCCCAGC GGCATCAAGC AAGAACCAGT CAGTTCAGAG 
TACACGGGGA CGAGTATGAA CTATGGAAGT GCATCGCCGT TGCAGCGAGT GCCGAGCGGG 
AAACCGCAAG CTCACGACGC TGGTGGAGTG AATGGAGAAC CAACAGGACT GGCCCTCGGA 
GCCAGAGCGC TGCTGCACGG TCTCCTGGCG CCCTCACCCT CGGTCAGGCA CCATCCCCTG 
TACACGGCGC CCAACACAGG CTCCCTCCCA CCTTCGCCAG CGGACAGTGG GGTATCGGAT 
GTGGAGTCGT CCTCGTCCGG AGCAGGATCC GCTGAGGACC TGAAGACGAG GCTTCAACCT 
CCCCCGCCGG CGCCCTTCCA CGCCCCCTTC CTGCCCTTCT ACCAGCATCA TCAGATCGCT 
TCCACATTGC AGCACGCCGC TGCACATCCT CGACCACCAG TGGGAGCGTT GAGCGATCGT 
GACGCATATG GATATGGTTA CGGCGGCGGG GGAGGCGGGA CTCACCACTT CGCAGCGCCT 
GCGCCCCCGC CGCTGCCGCA CGACGAGCTG CCATACTCCG TATTCGACTT CGGCGACTAC 
CAGCGCCACC ATCATCACAA CAAACTGAAG CCGAAGAAAA GGCCTCGCTC CGATGCACCT 
CCCACGCCGG GCGTCAAGCG CAAGAGCCGG GAGGGCTCCA CCACGTACCT GTGGGAGTTC 
CTGCTGAAGC TGCTGCAGGA CCGCGAGTAC TGCCCGCGCT TCATCAAGTG GACGAACCGC 
GAGAAGGGCG TGTTCAAGCT GGTCGACTCG AAGGCGGTGT CGCGACTCTG GGGCCTGCAC 
AAGAACAAGC CAGACATGAA CTACGAGACC ATGGGCCGCG CCCTGCGCTA TTACTACCAG 
CGCGGGATCC TCGCTAAGGT GGACGGCCAG CGCCTCGTCT ACCAGTTCGT GGACGTGCCC 
AAGGACATCG TCGAGATCGA CTGTTCGTTG GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001037444.1
CDS	889..2424
Translation	MPFIEDEWWSAENEGRMVDLSNCLQGQFQDAVVAAGGQAAAQLQQMASSLGELSQAELSNIVGGLTLEPESSEAADPDDILKQLGETAFDNFDTFFTDLTNATASGAPPIEIKQEENNNISSPASSSQLQGYYPQSQLHLQNNGQQRLQQLLRSGTNAINNAVNNNINNGRYNIASQNPLLAEKLSSTPSGIKQEPVSSEYTGTSMNYGSASPLQRVPSGKPQAHDAGGVNGEPTGLALGARALLHGLLAPSPSVRHHPLYTAPNTGSLPPSPADSGVSDVESSSSGAGSAEDLKTRLQPPPPAPFHAPFLPFYQHHQIASTLQHAAAHPRPPVGALSDRDAYGYGYGGGGGGTHHFAAPAPPPLPHDELPYSVFDFGDYQRHHHHNKLKPKKRPRSDAPPTPGVKRKSREGSTTYLWEFLLKLLQDREYCPRFIKWTNREKGVFKLVDSKAVSRLWGLHKNKPDMNYETMGRALRYYYQRGILAKVDGQRLVYQFVDVPKDIVEIDCSLA

Target ORF information:

RefSeq Version	NM_001043979.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori transcription factor E74 (E74), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043979.1

ORF Insert Sequence:

ATGCCATTTA TTGAAGACGA GTGGTGGTCC GCCGAGAATG AGGGCAGAAT GGTCGATCTC 
TCAAATTGCC TTCAGGGACA GTTTCAGGAC GCGGTAGTGG CTGCCGGCGG TCAGGCGGCC 
GCCCAGCTTC AGCAGATGGC CTCGTCACTA GGCGAGCTGT CGCAGGCCGA GCTGTCCAAC 
ATCGTGGGCG GCCTCACCCT GGAGCCGGAG AGCTCTGAAG CGGCAGACCC CGACGACATC 
CTGAAGCAGC TCGGGGAGAC CGCGTTCGAC AACTTCGATA CGTTTTTCAC GGATCTCACT 
AACGCCACCG CTTCGGGCGC ACCACCTATC GAGATAAAGC AAGAAGAGAA CAACAACATA 
TCGTCGCCAG CGTCCAGCAG TCAACTGCAA GGGTACTACC CTCAAAGTCA ATTACATTTA 
CAAAACAACG GGCAGCAAAG ATTGCAGCAA CTCCTTAGAT CGGGCACCAA CGCCATTAAC 
AATGCCGTTA ATAATAACAT TAACAATGGA AGATATAATA TTGCGTCGCA AAATCCGTTG 
CTCGCCGAGA AATTATCTTC AACGCCCAGC GGCATCAAGC AAGAACCAGT CAGTTCAGAG 
TACACGGGGA CGAGTATGAA CTATGGAAGT GCATCGCCGT TGCAGCGAGT GCCGAGCGGG 
AAACCGCAAG CTCACGACGC TGGTGGAGTG AATGGAGAAC CAACAGGACT GGCCCTCGGA 
GCCAGAGCGC TGCTGCACGG TCTCCTGGCG CCCTCACCCT CGGTCAGGCA CCATCCCCTG 
TACACGGCGC CCAACACAGG CTCCCTCCCA CCTTCGCCAG CGGACAGTGG GGTATCGGAT 
GTGGAGTCGT CCTCGTCCGG AGCAGGATCC GCTGAGGACC TGAAGACGAG GCTTCAACCT 
CCCCCGCCGG CGCCCTTCCA CGCCCCCTTC CTGCCCTTCT ACCAGCATCA TCAGATCGCT 
TCCACATTGC AGCACGCCGC TGCACATCCT CGACCACCAG TGGGAGCGTT GAGCGATCGT 
GACGCATATG GATATGGTTA CGGCGGCGGG GGAGGCGGGA CTCACCACTT CGCAGCGCCT 
GCGCCCCCGC CGCTGCCGCA CGACGAGCTG CCATACTCCG TATTCGACTT CGGCGACTAC 
CAGCGCCACC ATCATCACAA CAAACTGAAG CCGAAGAAAA GGCCTCGCTC CGATGCACCT 
CCCACGCCGG GCGTCAAGCG CAAGAGCCGG GAGGGCTCCA CCACGTACCT GTGGGAGTTC 
CTGCTGAAGC TGCTGCAGGA CCGCGAGTAC TGCCCGCGCT TCATCAAGTG GACGAACCGC 
GAGAAGGGCG TGTTCAAGCT GGTCGACTCG AAGGCGGTGT CGCGACTCTG GGGCCTGCAC 
AAGAACAAGC CAGACATGAA CTACGAGACC ATGGGCCGCG CCCTGCGCTA TTACTACCAG 
CGCGGGATCC TCGCTAAGGT GGACGGCCAG CGCCTCGTCT ACCAGTTCGT GGACGTGCCC 
AAGGACATCG TCGAGATCGA CTGTTCGTTG GCGTAG