LOC101744990 cDNA ORF clone, Bombyx mori(Domestic silkworm)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101744990
Entrez Gene ID	101744990
Full Name	DENN domain-containing protein 5B
Gene Type	protein-coding
Organism	Bombyx mori(Domestic silkworm)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012691791.1	XP_012547245.1	DENN domain-containing protein 5B isoform X3
XM_012691789.1	XP_012547243.1	DENN domain-containing protein 5B isoform X1
XM_012691789.2	XP_012547243.1	DENN domain-containing protein 5B
XM_012691790.1	XP_012547244.1	DENN domain-containing protein 5A isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC101744990 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101744990 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBg19778	XM_012691789.2 Latest version!	Bombyx mori DENN domain-containing protein 5B (LOC101744990), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBg19779	XM_012691790.1 Latest version!	Bombyx mori DENN domain-containing protein 5A (LOC101744990), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBg19780	XM_012691791.1 Latest version!	Bombyx mori DENN domain-containing protein 5A (LOC101744990), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OBg19778	XM_012691789.1	Bombyx mori DENN domain-containing protein 5A (LOC101744990), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101744990 ( XM_012691789.1 ) cDNA ORF clone, Bombyx mori(Domestic silkworm) -> XP_012547243.1 Bombyx mori DENN domain-containing protein 5A (LOC101744990), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBg19778
Clone ID Related Accession (Same CDS sequence)	XM_012691789.1 , XM_012691789.2
Accession Version	XM_012691789.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433347200000
Organism	Bombyx mori(Domestic silkworm)
Product	DENN domain-containing protein 5B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004582008.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTCC CTGATTTGTC TGTTAGTTGT CGTTTTGCTG ATTATTTTGC TATATGTGGA 
CTTGATTTCG ATTCTGGATT AGAACCTGAT AGACTATGTG GTGATAATTT ACACGTATCT 
CCTCTCGACC GATCCTATAA AGGAAAAATT TTAGCTCACT ATCCTGAAAA TGTTCCTAGT 
AATCAATTTG ATGAACATGC AACCTGTATG CTCTGTCTAC CAGCTGGCCT CCAATTTAGA 
ACCCAAAAGC ATTCATTGGA GCCCCAATTT CACAGTTTTG TAGTAACTAG AGAAAATGCA 
TCTAGATATT ATGGATTTAG CCTTATATTT TATGAAGAGG TCAGAAATAA AAACATCTGT 
AGTGCTATGC ATACTTTACA GGCGATGTAC ATTACAGAAC TCTCTAGTGG CCAGACTCCA 
CCAGGCCATA GAAAAAGCTC TGGTAAGGAG AGCTCACGAT CCCTCCCCAG ATCGTTCAAA 
CTTACGCCTC ATACTGGTGC TGCTCTAACA TACTTTGATA TAGCCAAAGA TAAACTCTAT 
GTAGCTAAAA GTATAGCACT GATTTGTCAA TACCCCTTTG TAAGAGTTGC CCAAATGTTT 
TTAGAAAACT TGTTCAGATC CTTACCAAGA CAACCAGGAC CTGGTCTTAG CCCAGAATCT 
TATGTTTATA ATTTACTTTA CGAAGTACCA GTGCCCTTGC CAGGTCAAGC TTTAAGACTT 
TATGTGCCAC CTCCTCAACC ACATCTTGAA GCCATTCCAG TTGTGATAAG AATGCCAAAT 
CCTCCAGAAG AGTTACCACT GTTAGACTAT TCGTTGCGTG TCATGTTTTC TAATTTAGGC 
GTTGAACTAG TAGTACAATT GTTCACGTGT GTTCTACTTG AACATCAAGT ATTACTTAGA 
TCAACTGATT ACAACAAGCT GATGCTGGTT TCTGAATGTA TGGTATCGCT ATTGCTTCCC 
TTCACTTGGG CGCACGTCTA CGTACCGATA CTACCGGCTC CTTTATATCA CTTCCTCGAC 
GCGCCCGTAC CATTTGTCAT GGGTTTACAT ACAGACAGCG CGGCCGTTTG CGCCAACGGA 
CCCAGAAGCA TTGCCCTTTG TTCTGAAGCA GCACTGTGTC TGGTAGACAT CGACAAACCC 
GACATACAAC TACCAGAAGA ATTGCCGATA TTCCCACACA GAACATCCTT CATTGAGGAA 
CTCAATCATT TAATGGATAA ATATCAGATT CAGAGACCCG AGACGGAACC AACCAAATTA 
AGTGCGCCCC TAAACGGAAC ATCGTATAGG CACATGAATG ACAACATGAG CAGCAGTTGT 
ACTTTACCAT CAGGCGGCTT ACGCAGGAAG CACTCGTTTC ACGACGTGCT AGAGTGGGAA 
GAAAGTAGGC CGTTGAACGC CTCCCCTCCC GCCTCGCCCA CCCGCCGCGC TCCCCTGCGG 
CCCGACGCCT TGCAGCGTAT CGCCGATATC GTCAAGCGTA CAGGTGTCAA CATTGACGAC 
GTGGATGCCG AAACAGTGAT GCCAACTACT AAAAAGAAAA TTCTCAACGA TGAAGAGCAA 
TATAATGAAG ATATGCGTTT TAACAGTGCT GTTAGAGAAA CGTTTCTCAA TAGATTTGTT 
CATATATTTT TGATGTATGA AAATTTCGTT ATCATGCCCG ATCAGGACCG CGAGTCATGG 
CTGAGCTCAC GCGAATCGAT GGTCAACTTT GACAAGGCGT CCTTCCTCTC CGACCAGCCT 
CAGCGTCACC GACCATTCCT GTCACGCTTC CTCGAAACCC AAATGTTCGC CACGCTCATC 
GACAACAAGA TCATGGCTAA TTGGGGGGAA TATGACGTTA ATTTGCAAGT CTTCGAATAT 
CGGACTAGGA TCGTCAAAAC GAACAGTCTA AAGCGAATAA AAAATGCGAA ATGGCAAGTG 
AAAGACTGTC CGCCAGAGTT GTTGCTCGGA GACTTCAGCA GACGGTTGTC TACTGAAGTC 
ACGCCTGCAG CCATTGCACA GAATAACAGG ACATTTGTGG AGAAACTCCT TAAGGAATGT 
AAGAGCAAAA CAAAACGAAT GCTATTGGAG AAGATGGGTA CAGAAGAGTC TAACTTAGGC 
TTCGGGAGCG AAGTGTCAAT TACTGGTGTC GAGGAAAGCA CTATGATTGC GTCCCTGTGT 
GATTTATTAG AACGACTGTG GTCACATGGC CTACAACACA AAATGGGAAA GTCAGCGCTC 
TGGATGCATC TCATTAACTA CCAAGAGCTG GAACAATGCA GCAATTCAAC TAAACCTATC 
GATCCCAATT ATCTAACACC AGCATTAGCT TGGTGTGTAC TACGGAAACG TCTTGACTAC 
TTATCATCGG TTGGAGCGGA AGTTGAAGCT CAACAAGCAG CCAGTGTGAG TCCGCGATCA 
CGGGACAGCT CCCGCGGCGG ATCCCGCGAT AGCTCTCGGG ACCGTCTGAG TAATTCAGGA 
ACCCTAGAAC GCAAATCGAC TCAGCCCGTT AATCCTTTGC GGCCCTTGCC CGAGAGCTTG 
ACCTTTGATA TGAGGAATGT TCAAGCTATG ACTGATATAA AAACCGAAAT AGGCTACGCT 
CGTGCTTGGG TTCGTTTGTC GCTTGAGAAG AAGCTCCTCT CGAAACATCT CCGTGAATTA 
TTAGCCGATA CCACACTATT GCGCTCTCAA TACAAAAGAA CCGCTTTTCT GCGCTGCGAA 
GAGGAAAGGG AACAATTTTT ATATCACTTA CTGACTTTGA ATGCCGTTGA CTACTATTGC 
TTCACGAACA CGTATCCTAC AACAAAACTT CCATATCGCG TATTAATAAT GCCTTCTCGC 
AAAGCTAGTC AAACGACGGC CAACTGTTGG ATCTCAATCT CTGGTATGAA CGGAGAGTCG 
ACTCCGAAAA TTCCCATACC TCGTAACACA AACGAATTCG TTTTCCATCA CAAGAATCTT 
GGGACATTAT CAACCTTGAG AATCGGTCAC GATAATTCTG GTTTGTCGCC GCGGTGGTTA 
CTCGACCAAG TTTATGTCAG AAATGAAGTG ACAGGACATA CTTACACGTA TCCTTGTAAC 
CGCTGGCTGG GGAGTGGAGT GGACGACGGC TCCACCGAGC GGCTGCTGGT GGCGGCGCGC 
GTGCGCAGCG AGCACACGCC GCGCTCCCCC GCGCCCCCCG CCGCCCTGCA CCCGCACACT 
CCCACCACGC ACCTCTCGAC CTCGACCATC CAGCACATGA TCGGTGATTG CGTGAACGCC 
ATTGTGAAAT GGCACTATCA GTCAAAAAGA GATAAGGACG CGAACTCGCT AAGTGTCTTA 
CTGTGCGGAG AAAATGGCTT GGTCAAGTGC TTGGAGCAAG CTTTCCTCTG TGGCTTCAAA 
TCGGCGCGCT TCTTCGGCAA GAATTTTTTC ATATGGGATT ATTTCGCTAG AGTCAAAGAC 
GAGTTCAAAA TGAGCCTGTG CGACGAGCCC AGTACGATGA ACAACAAGAA CTGCGGCGGC 
GTATCCTACA ACTGCAGACA GGACCAAGAG CACAGAGCCA TCTGGCGATG CTACTGCCAT 
CTCATGGACG AGGTCAACAG TGTGGGCCGC TCGCTGGGGA AGGACGGGAA GTTTCAACTC 
TTCATATGTC TAAGTTTACG GGAACACTGG CTTCATCGAA TGCTGGTGCC TATGTCATTG 
ACTCGTGTCA CAGCTGAGAT GTACGAGGAG CAAAGTTTTC TGCGCAAACA CGGGCTTCTC 
ACGTTTCTGA AACAAATACT CGAGCCTTTG GACGAATTTG ACATTGTACT CGAAAACTCT 
CTCACTCAAG GCATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012547243.1
CDS	219..4016
Translation	MSVPDLSVSCRFADYFAICGLDFDSGLEPDRLCGDNLHVSPLDRSYKGKILAHYPENVPSNQFDEHATCMLCLPAGLQFRTQKHSLEPQFHSFVVTRENASRYYGFSLIFYEEVRNKNICSAMHTLQAMYITELSSGQTPPGHRKSSGKESSRSLPRSFKLTPHTGAALTYFDIAKDKLYVAKSIALICQYPFVRVAQMFLENLFRSLPRQPGPGLSPESYVYNLLYEVPVPLPGQALRLYVPPPQPHLEAIPVVIRMPNPPEELPLLDYSLRVMFSNLGVELVVQLFTCVLLEHQVLLRSTDYNKLMLVSECMVSLLLPFTWAHVYVPILPAPLYHFLDAPVPFVMGLHTDSAAVCANGPRSIALCSEAALCLVDIDKPDIQLPEELPIFPHRTSFIEELNHLMDKYQIQRPETEPTKLSAPLNGTSYRHMNDNMSSSCTLPSGGLRRKHSFHDVLEWEESRPLNASPPASPTRRAPLRPDALQRIADIVKRTGVNIDDVDAETVMPTTKKKILNDEEQYNEDMRFNSAVRETFLNRFVHIFLMYENFVIMPDQDRESWLSSRESMVNFDKASFLSDQPQRHRPFLSRFLETQMFATLIDNKIMANWGEYDVNLQVFEYRTRIVKTNSLKRIKNAKWQVKDCPPELLLGDFSRRLSTEVTPAAIAQNNRTFVEKLLKECKSKTKRMLLEKMGTEESNLGFGSEVSITGVEESTMIASLCDLLERLWSHGLQHKMGKSALWMHLINYQELEQCSNSTKPIDPNYLTPALAWCVLRKRLDYLSSVGAEVEAQQAASVSPRSRDSSRGGSRDSSRDRLSNSGTLERKSTQPVNPLRPLPESLTFDMRNVQAMTDIKTEIGYARAWVRLSLEKKLLSKHLRELLADTTLLRSQYKRTAFLRCEEEREQFLYHLLTLNAVDYYCFTNTYPTTKLPYRVLIMPSRKASQTTANCWISISGMNGESTPKIPIPRNTNEFVFHHKNLGTLSTLRIGHDNSGLSPRWLLDQVYVRNEVTGHTYTYPCNRWLGSGVDDGSTERLLVAARVRSEHTPRSPAPPAALHPHTPTTHLSTSTIQHMIGDCVNAIVKWHYQSKRDKDANSLSVLLCGENGLVKCLEQAFLCGFKSARFFGKNFFIWDYFARVKDEFKMSLCDEPSTMNNKNCGGVSYNCRQDQEHRAIWRCYCHLMDEVNSVGRSLGKDGKFQLFICLSLREHWLHRMLVPMSLTRVTAEMYEEQSFLRKHGLLTFLKQILEPLDEFDIVLENSLTQGI

Target ORF information:

RefSeq Version	XM_012691789.1
Organism	Bombyx mori(Domestic silkworm)
Definition	Bombyx mori DENN domain-containing protein 5A (LOC101744990), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012691789.1

ORF Insert Sequence:

ATGTCTGTCC CTGATTTGTC TGTTAGTTGT CGTTTTGCTG ATTATTTTGC TATATGTGGA 
CTTGATTTCG ATTCTGGATT AGAACCTGAT AGACTATGTG GTGATAATTT ACACGTATCT 
CCTCTCGACC GATCCTATAA AGGAAAAATT TTAGCTCACT ATCCTGAAAA TGTTCCTAGT 
AATCAATTTG ATGAACATGC AACCTGTATG CTCTGTCTAC CAGCTGGCCT CCAATTTAGA 
ACCCAAAAGC ATTCATTGGA GCCCCAATTT CACAGTTTTG TAGTAACTAG AGAAAATGCA 
TCTAGATATT ATGGATTTAG CCTTATATTT TATGAAGAGG TCAGAAATAA AAACATCTGT 
AGTGCTATGC ATACTTTACA GGCGATGTAC ATTACAGAAC TCTCTAGTGG CCAGACTCCA 
CCAGGCCATA GAAAAAGCTC TGGTAAGGAG AGCTCACGAT CCCTCCCCAG ATCGTTCAAA 
CTTACGCCTC ATACTGGTGC TGCTCTAACA TACTTTGATA TAGCCAAAGA TAAACTCTAT 
GTAGCTAAAA GTATAGCACT GATTTGTCAA TACCCCTTTG TAAGAGTTGC CCAAATGTTT 
TTAGAAAACT TGTTCAGATC CTTACCAAGA CAACCAGGAC CTGGTCTTAG CCCAGAATCT 
TATGTTTATA ATTTACTTTA CGAAGTACCA GTGCCCTTGC CAGGTCAAGC TTTAAGACTT 
TATGTGCCAC CTCCTCAACC ACATCTTGAA GCCATTCCAG TTGTGATAAG AATGCCAAAT 
CCTCCAGAAG AGTTACCACT GTTAGACTAT TCGTTGCGTG TCATGTTTTC TAATTTAGGC 
GTTGAACTAG TAGTACAATT GTTCACGTGT GTTCTACTTG AACATCAAGT ATTACTTAGA 
TCAACTGATT ACAACAAGCT GATGCTGGTT TCTGAATGTA TGGTATCGCT ATTGCTTCCC 
TTCACTTGGG CGCACGTCTA CGTACCGATA CTACCGGCTC CTTTATATCA CTTCCTCGAC 
GCGCCCGTAC CATTTGTCAT GGGTTTACAT ACAGACAGCG CGGCCGTTTG CGCCAACGGA 
CCCAGAAGCA TTGCCCTTTG TTCTGAAGCA GCACTGTGTC TGGTAGACAT CGACAAACCC 
GACATACAAC TACCAGAAGA ATTGCCGATA TTCCCACACA GAACATCCTT CATTGAGGAA 
CTCAATCATT TAATGGATAA ATATCAGATT CAGAGACCCG AGACGGAACC AACCAAATTA 
AGTGCGCCCC TAAACGGAAC ATCGTATAGG CACATGAATG ACAACATGAG CAGCAGTTGT 
ACTTTACCAT CAGGCGGCTT ACGCAGGAAG CACTCGTTTC ACGACGTGCT AGAGTGGGAA 
GAAAGTAGGC CGTTGAACGC CTCCCCTCCC GCCTCGCCCA CCCGCCGCGC TCCCCTGCGG 
CCCGACGCCT TGCAGCGTAT CGCCGATATC GTCAAGCGTA CAGGTGTCAA CATTGACGAC 
GTGGATGCCG AAACAGTGAT GCCAACTACT AAAAAGAAAA TTCTCAACGA TGAAGAGCAA 
TATAATGAAG ATATGCGTTT TAACAGTGCT GTTAGAGAAA CGTTTCTCAA TAGATTTGTT 
CATATATTTT TGATGTATGA AAATTTCGTT ATCATGCCCG ATCAGGACCG CGAGTCATGG 
CTGAGCTCAC GCGAATCGAT GGTCAACTTT GACAAGGCGT CCTTCCTCTC CGACCAGCCT 
CAGCGTCACC GACCATTCCT GTCACGCTTC CTCGAAACCC AAATGTTCGC CACGCTCATC 
GACAACAAGA TCATGGCTAA TTGGGGGGAA TATGACGTTA ATTTGCAAGT CTTCGAATAT 
CGGACTAGGA TCGTCAAAAC GAACAGTCTA AAGCGAATAA AAAATGCGAA ATGGCAAGTG 
AAAGACTGTC CGCCAGAGTT GTTGCTCGGA GACTTCAGCA GACGGTTGTC TACTGAAGTC 
ACGCCTGCAG CCATTGCACA GAATAACAGG ACATTTGTGG AGAAACTCCT TAAGGAATGT 
AAGAGCAAAA CAAAACGAAT GCTATTGGAG AAGATGGGTA CAGAAGAGTC TAACTTAGGC 
TTCGGGAGCG AAGTGTCAAT TACTGGTGTC GAGGAAAGCA CTATGATTGC GTCCCTGTGT 
GATTTATTAG AACGACTGTG GTCACATGGC CTACAACACA AAATGGGAAA GTCAGCGCTC 
TGGATGCATC TCATTAACTA CCAAGAGCTG GAACAATGCA GCAATTCAAC TAAACCTATC 
GATCCCAATT ATCTAACACC AGCATTAGCT TGGTGTGTAC TACGGAAACG TCTTGACTAC 
TTATCATCGG TTGGAGCGGA AGTTGAAGCT CAACAAGCAG CCAGTGTGAG TCCGCGATCA 
CGGGACAGCT CCCGCGGCGG ATCCCGCGAT AGCTCTCGGG ACCGTCTGAG TAATTCAGGA 
ACCCTAGAAC GCAAATCGAC TCAGCCCGTT AATCCTTTGC GGCCCTTGCC CGAGAGCTTG 
ACCTTTGATA TGAGGAATGT TCAAGCTATG ACTGATATAA AAACCGAAAT AGGCTACGCT 
CGTGCTTGGG TTCGTTTGTC GCTTGAGAAG AAGCTCCTCT CGAAACATCT CCGTGAATTA 
TTAGCCGATA CCACACTATT GCGCTCTCAA TACAAAAGAA CCGCTTTTCT GCGCTGCGAA 
GAGGAAAGGG AACAATTTTT ATATCACTTA CTGACTTTGA ATGCCGTTGA CTACTATTGC 
TTCACGAACA CGTATCCTAC AACAAAACTT CCATATCGCG TATTAATAAT GCCTTCTCGC 
AAAGCTAGTC AAACGACGGC CAACTGTTGG ATCTCAATCT CTGGTATGAA CGGAGAGTCG 
ACTCCGAAAA TTCCCATACC TCGTAACACA AACGAATTCG TTTTCCATCA CAAGAATCTT 
GGGACATTAT CAACCTTGAG AATCGGTCAC GATAATTCTG GTTTGTCGCC GCGGTGGTTA 
CTCGACCAAG TTTATGTCAG AAATGAAGTG ACAGGACATA CTTACACGTA TCCTTGTAAC 
CGCTGGCTGG GGAGTGGAGT GGACGACGGC TCCACCGAGC GGCTGCTGGT GGCGGCGCGC 
GTGCGCAGCG AGCACACGCC GCGCTCCCCC GCGCCCCCCG CCGCCCTGCA CCCGCACACT 
CCCACCACGC ACCTCTCGAC CTCGACCATC CAGCACATGA TCGGTGATTG CGTGAACGCC 
ATTGTGAAAT GGCACTATCA GTCAAAAAGA GATAAGGACG CGAACTCGCT AAGTGTCTTA 
CTGTGCGGAG AAAATGGCTT GGTCAAGTGC TTGGAGCAAG CTTTCCTCTG TGGCTTCAAA 
TCGGCGCGCT TCTTCGGCAA GAATTTTTTC ATATGGGATT ATTTCGCTAG AGTCAAAGAC 
GAGTTCAAAA TGAGCCTGTG CGACGAGCCC AGTACGATGA ACAACAAGAA CTGCGGCGGC 
GTATCCTACA ACTGCAGACA GGACCAAGAG CACAGAGCCA TCTGGCGATG CTACTGCCAT 
CTCATGGACG AGGTCAACAG TGTGGGCCGC TCGCTGGGGA AGGACGGGAA GTTTCAACTC 
TTCATATGTC TAAGTTTACG GGAACACTGG CTTCATCGAA TGCTGGTGCC TATGTCATTG 
ACTCGTGTCA CAGCTGAGAT GTACGAGGAG CAAAGTTTTC TGCGCAAACA CGGGCTTCTC 
ACGTTTCTGA AACAAATACT CGAGCCTTTG GACGAATTTG ACATTGTACT CGAAAACTCT 
CTCACTCAAG GCATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101744990 ( XM_012691789.2 ) cDNA ORF clone, Bombyx mori(Domestic silkworm) -> XP_012547243.1 Bombyx mori DENN domain-containing protein 5B (LOC101744990), mRNA.

Price & Availability↑

CloneID	OBg19778
Clone ID Related Accession (Same CDS sequence)	XM_012691789.1 , XM_012691789.2
Accession Version	XM_012691789.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1496505600000
Organism	Bombyx mori(domestic silkworm)
Product	DENN domain-containing protein 5B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004582008.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 5, 2017 this sequence version replaced XM_012691789.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTCC CTGATTTGTC TGTTAGTTGT CGTTTTGCTG ATTATTTTGC TATATGTGGA 
CTTGATTTCG ATTCTGGATT AGAACCTGAT AGACTATGTG GTGATAATTT ACACGTATCT 
CCTCTCGACC GATCCTATAA AGGAAAAATT TTAGCTCACT ATCCTGAAAA TGTTCCTAGT 
AATCAATTTG ATGAACATGC AACCTGTATG CTCTGTCTAC CAGCTGGCCT CCAATTTAGA 
ACCCAAAAGC ATTCATTGGA GCCCCAATTT CACAGTTTTG TAGTAACTAG AGAAAATGCA 
TCTAGATATT ATGGATTTAG CCTTATATTT TATGAAGAGG TCAGAAATAA AAACATCTGT 
AGTGCTATGC ATACTTTACA GGCGATGTAC ATTACAGAAC TCTCTAGTGG CCAGACTCCA 
CCAGGCCATA GAAAAAGCTC TGGTAAGGAG AGCTCACGAT CCCTCCCCAG ATCGTTCAAA 
CTTACGCCTC ATACTGGTGC TGCTCTAACA TACTTTGATA TAGCCAAAGA TAAACTCTAT 
GTAGCTAAAA GTATAGCACT GATTTGTCAA TACCCCTTTG TAAGAGTTGC CCAAATGTTT 
TTAGAAAACT TGTTCAGATC CTTACCAAGA CAACCAGGAC CTGGTCTTAG CCCAGAATCT 
TATGTTTATA ATTTACTTTA CGAAGTACCA GTGCCCTTGC CAGGTCAAGC TTTAAGACTT 
TATGTGCCAC CTCCTCAACC ACATCTTGAA GCCATTCCAG TTGTGATAAG AATGCCAAAT 
CCTCCAGAAG AGTTACCACT GTTAGACTAT TCGTTGCGTG TCATGTTTTC TAATTTAGGC 
GTTGAACTAG TAGTACAATT GTTCACGTGT GTTCTACTTG AACATCAAGT ATTACTTAGA 
TCAACTGATT ACAACAAGCT GATGCTGGTT TCTGAATGTA TGGTATCGCT ATTGCTTCCC 
TTCACTTGGG CGCACGTCTA CGTACCGATA CTACCGGCTC CTTTATATCA CTTCCTCGAC 
GCGCCCGTAC CATTTGTCAT GGGTTTACAT ACAGACAGCG CGGCCGTTTG CGCCAACGGA 
CCCAGAAGCA TTGCCCTTTG TTCTGAAGCA GCACTGTGTC TGGTAGACAT CGACAAACCC 
GACATACAAC TACCAGAAGA ATTGCCGATA TTCCCACACA GAACATCCTT CATTGAGGAA 
CTCAATCATT TAATGGATAA ATATCAGATT CAGAGACCCG AGACGGAACC AACCAAATTA 
AGTGCGCCCC TAAACGGAAC ATCGTATAGG CACATGAATG ACAACATGAG CAGCAGTTGT 
ACTTTACCAT CAGGCGGCTT ACGCAGGAAG CACTCGTTTC ACGACGTGCT AGAGTGGGAA 
GAAAGTAGGC CGTTGAACGC CTCCCCTCCC GCCTCGCCCA CCCGCCGCGC TCCCCTGCGG 
CCCGACGCCT TGCAGCGTAT CGCCGATATC GTCAAGCGTA CAGGTGTCAA CATTGACGAC 
GTGGATGCCG AAACAGTGAT GCCAACTACT AAAAAGAAAA TTCTCAACGA TGAAGAGCAA 
TATAATGAAG ATATGCGTTT TAACAGTGCT GTTAGAGAAA CGTTTCTCAA TAGATTTGTT 
CATATATTTT TGATGTATGA AAATTTCGTT ATCATGCCCG ATCAGGACCG CGAGTCATGG 
CTGAGCTCAC GCGAATCGAT GGTCAACTTT GACAAGGCGT CCTTCCTCTC CGACCAGCCT 
CAGCGTCACC GACCATTCCT GTCACGCTTC CTCGAAACCC AAATGTTCGC CACGCTCATC 
GACAACAAGA TCATGGCTAA TTGGGGGGAA TATGACGTTA ATTTGCAAGT CTTCGAATAT 
CGGACTAGGA TCGTCAAAAC GAACAGTCTA AAGCGAATAA AAAATGCGAA ATGGCAAGTG 
AAAGACTGTC CGCCAGAGTT GTTGCTCGGA GACTTCAGCA GACGGTTGTC TACTGAAGTC 
ACGCCTGCAG CCATTGCACA GAATAACAGG ACATTTGTGG AGAAACTCCT TAAGGAATGT 
AAGAGCAAAA CAAAACGAAT GCTATTGGAG AAGATGGGTA CAGAAGAGTC TAACTTAGGC 
TTCGGGAGCG AAGTGTCAAT TACTGGTGTC GAGGAAAGCA CTATGATTGC GTCCCTGTGT 
GATTTATTAG AACGACTGTG GTCACATGGC CTACAACACA AAATGGGAAA GTCAGCGCTC 
TGGATGCATC TCATTAACTA CCAAGAGCTG GAACAATGCA GCAATTCAAC TAAACCTATC 
GATCCCAATT ATCTAACACC AGCATTAGCT TGGTGTGTAC TACGGAAACG TCTTGACTAC 
TTATCATCGG TTGGAGCGGA AGTTGAAGCT CAACAAGCAG CCAGTGTGAG TCCGCGATCA 
CGGGACAGCT CCCGCGGCGG ATCCCGCGAT AGCTCTCGGG ACCGTCTGAG TAATTCAGGA 
ACCCTAGAAC GCAAATCGAC TCAGCCCGTT AATCCTTTGC GGCCCTTGCC CGAGAGCTTG 
ACCTTTGATA TGAGGAATGT TCAAGCTATG ACTGATATAA AAACCGAAAT AGGCTACGCT 
CGTGCTTGGG TTCGTTTGTC GCTTGAGAAG AAGCTCCTCT CGAAACATCT CCGTGAATTA 
TTAGCCGATA CCACACTATT GCGCTCTCAA TACAAAAGAA CCGCTTTTCT GCGCTGCGAA 
GAGGAAAGGG AACAATTTTT ATATCACTTA CTGACTTTGA ATGCCGTTGA CTACTATTGC 
TTCACGAACA CGTATCCTAC AACAAAACTT CCATATCGCG TATTAATAAT GCCTTCTCGC 
AAAGCTAGTC AAACGACGGC CAACTGTTGG ATCTCAATCT CTGGTATGAA CGGAGAGTCG 
ACTCCGAAAA TTCCCATACC TCGTAACACA AACGAATTCG TTTTCCATCA CAAGAATCTT 
GGGACATTAT CAACCTTGAG AATCGGTCAC GATAATTCTG GTTTGTCGCC GCGGTGGTTA 
CTCGACCAAG TTTATGTCAG AAATGAAGTG ACAGGACATA CTTACACGTA TCCTTGTAAC 
CGCTGGCTGG GGAGTGGAGT GGACGACGGC TCCACCGAGC GGCTGCTGGT GGCGGCGCGC 
GTGCGCAGCG AGCACACGCC GCGCTCCCCC GCGCCCCCCG CCGCCCTGCA CCCGCACACT 
CCCACCACGC ACCTCTCGAC CTCGACCATC CAGCACATGA TCGGTGATTG CGTGAACGCC 
ATTGTGAAAT GGCACTATCA GTCAAAAAGA GATAAGGACG CGAACTCGCT AAGTGTCTTA 
CTGTGCGGAG AAAATGGCTT GGTCAAGTGC TTGGAGCAAG CTTTCCTCTG TGGCTTCAAA 
TCGGCGCGCT TCTTCGGCAA GAATTTTTTC ATATGGGATT ATTTCGCTAG AGTCAAAGAC 
GAGTTCAAAA TGAGCCTGTG CGACGAGCCC AGTACGATGA ACAACAAGAA CTGCGGCGGC 
GTATCCTACA ACTGCAGACA GGACCAAGAG CACAGAGCCA TCTGGCGATG CTACTGCCAT 
CTCATGGACG AGGTCAACAG TGTGGGCCGC TCGCTGGGGA AGGACGGGAA GTTTCAACTC 
TTCATATGTC TAAGTTTACG GGAACACTGG CTTCATCGAA TGCTGGTGCC TATGTCATTG 
ACTCGTGTCA CAGCTGAGAT GTACGAGGAG CAAAGTTTTC TGCGCAAACA CGGGCTTCTC 
ACGTTTCTGA AACAAATACT CGAGCCTTTG GACGAATTTG ACATTGTACT CGAAAACTCT 
CTCACTCAAG GCATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012547243.1
CDS	245..4042
Translation	MSVPDLSVSCRFADYFAICGLDFDSGLEPDRLCGDNLHVSPLDRSYKGKILAHYPENVPSNQFDEHATCMLCLPAGLQFRTQKHSLEPQFHSFVVTRENASRYYGFSLIFYEEVRNKNICSAMHTLQAMYITELSSGQTPPGHRKSSGKESSRSLPRSFKLTPHTGAALTYFDIAKDKLYVAKSIALICQYPFVRVAQMFLENLFRSLPRQPGPGLSPESYVYNLLYEVPVPLPGQALRLYVPPPQPHLEAIPVVIRMPNPPEELPLLDYSLRVMFSNLGVELVVQLFTCVLLEHQVLLRSTDYNKLMLVSECMVSLLLPFTWAHVYVPILPAPLYHFLDAPVPFVMGLHTDSAAVCANGPRSIALCSEAALCLVDIDKPDIQLPEELPIFPHRTSFIEELNHLMDKYQIQRPETEPTKLSAPLNGTSYRHMNDNMSSSCTLPSGGLRRKHSFHDVLEWEESRPLNASPPASPTRRAPLRPDALQRIADIVKRTGVNIDDVDAETVMPTTKKKILNDEEQYNEDMRFNSAVRETFLNRFVHIFLMYENFVIMPDQDRESWLSSRESMVNFDKASFLSDQPQRHRPFLSRFLETQMFATLIDNKIMANWGEYDVNLQVFEYRTRIVKTNSLKRIKNAKWQVKDCPPELLLGDFSRRLSTEVTPAAIAQNNRTFVEKLLKECKSKTKRMLLEKMGTEESNLGFGSEVSITGVEESTMIASLCDLLERLWSHGLQHKMGKSALWMHLINYQELEQCSNSTKPIDPNYLTPALAWCVLRKRLDYLSSVGAEVEAQQAASVSPRSRDSSRGGSRDSSRDRLSNSGTLERKSTQPVNPLRPLPESLTFDMRNVQAMTDIKTEIGYARAWVRLSLEKKLLSKHLRELLADTTLLRSQYKRTAFLRCEEEREQFLYHLLTLNAVDYYCFTNTYPTTKLPYRVLIMPSRKASQTTANCWISISGMNGESTPKIPIPRNTNEFVFHHKNLGTLSTLRIGHDNSGLSPRWLLDQVYVRNEVTGHTYTYPCNRWLGSGVDDGSTERLLVAARVRSEHTPRSPAPPAALHPHTPTTHLSTSTIQHMIGDCVNAIVKWHYQSKRDKDANSLSVLLCGENGLVKCLEQAFLCGFKSARFFGKNFFIWDYFARVKDEFKMSLCDEPSTMNNKNCGGVSYNCRQDQEHRAIWRCYCHLMDEVNSVGRSLGKDGKFQLFICLSLREHWLHRMLVPMSLTRVTAEMYEEQSFLRKHGLLTFLKQILEPLDEFDIVLENSLTQGI

Target ORF information:

RefSeq Version	XM_012691789.2
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori DENN domain-containing protein 5B (LOC101744990), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012691789.2

ORF Insert Sequence:

ATGTCTGTCC CTGATTTGTC TGTTAGTTGT CGTTTTGCTG ATTATTTTGC TATATGTGGA 
CTTGATTTCG ATTCTGGATT AGAACCTGAT AGACTATGTG GTGATAATTT ACACGTATCT 
CCTCTCGACC GATCCTATAA AGGAAAAATT TTAGCTCACT ATCCTGAAAA TGTTCCTAGT 
AATCAATTTG ATGAACATGC AACCTGTATG CTCTGTCTAC CAGCTGGCCT CCAATTTAGA 
ACCCAAAAGC ATTCATTGGA GCCCCAATTT CACAGTTTTG TAGTAACTAG AGAAAATGCA 
TCTAGATATT ATGGATTTAG CCTTATATTT TATGAAGAGG TCAGAAATAA AAACATCTGT 
AGTGCTATGC ATACTTTACA GGCGATGTAC ATTACAGAAC TCTCTAGTGG CCAGACTCCA 
CCAGGCCATA GAAAAAGCTC TGGTAAGGAG AGCTCACGAT CCCTCCCCAG ATCGTTCAAA 
CTTACGCCTC ATACTGGTGC TGCTCTAACA TACTTTGATA TAGCCAAAGA TAAACTCTAT 
GTAGCTAAAA GTATAGCACT GATTTGTCAA TACCCCTTTG TAAGAGTTGC CCAAATGTTT 
TTAGAAAACT TGTTCAGATC CTTACCAAGA CAACCAGGAC CTGGTCTTAG CCCAGAATCT 
TATGTTTATA ATTTACTTTA CGAAGTACCA GTGCCCTTGC CAGGTCAAGC TTTAAGACTT 
TATGTGCCAC CTCCTCAACC ACATCTTGAA GCCATTCCAG TTGTGATAAG AATGCCAAAT 
CCTCCAGAAG AGTTACCACT GTTAGACTAT TCGTTGCGTG TCATGTTTTC TAATTTAGGC 
GTTGAACTAG TAGTACAATT GTTCACGTGT GTTCTACTTG AACATCAAGT ATTACTTAGA 
TCAACTGATT ACAACAAGCT GATGCTGGTT TCTGAATGTA TGGTATCGCT ATTGCTTCCC 
TTCACTTGGG CGCACGTCTA CGTACCGATA CTACCGGCTC CTTTATATCA CTTCCTCGAC 
GCGCCCGTAC CATTTGTCAT GGGTTTACAT ACAGACAGCG CGGCCGTTTG CGCCAACGGA 
CCCAGAAGCA TTGCCCTTTG TTCTGAAGCA GCACTGTGTC TGGTAGACAT CGACAAACCC 
GACATACAAC TACCAGAAGA ATTGCCGATA TTCCCACACA GAACATCCTT CATTGAGGAA 
CTCAATCATT TAATGGATAA ATATCAGATT CAGAGACCCG AGACGGAACC AACCAAATTA 
AGTGCGCCCC TAAACGGAAC ATCGTATAGG CACATGAATG ACAACATGAG CAGCAGTTGT 
ACTTTACCAT CAGGCGGCTT ACGCAGGAAG CACTCGTTTC ACGACGTGCT AGAGTGGGAA 
GAAAGTAGGC CGTTGAACGC CTCCCCTCCC GCCTCGCCCA CCCGCCGCGC TCCCCTGCGG 
CCCGACGCCT TGCAGCGTAT CGCCGATATC GTCAAGCGTA CAGGTGTCAA CATTGACGAC 
GTGGATGCCG AAACAGTGAT GCCAACTACT AAAAAGAAAA TTCTCAACGA TGAAGAGCAA 
TATAATGAAG ATATGCGTTT TAACAGTGCT GTTAGAGAAA CGTTTCTCAA TAGATTTGTT 
CATATATTTT TGATGTATGA AAATTTCGTT ATCATGCCCG ATCAGGACCG CGAGTCATGG 
CTGAGCTCAC GCGAATCGAT GGTCAACTTT GACAAGGCGT CCTTCCTCTC CGACCAGCCT 
CAGCGTCACC GACCATTCCT GTCACGCTTC CTCGAAACCC AAATGTTCGC CACGCTCATC 
GACAACAAGA TCATGGCTAA TTGGGGGGAA TATGACGTTA ATTTGCAAGT CTTCGAATAT 
CGGACTAGGA TCGTCAAAAC GAACAGTCTA AAGCGAATAA AAAATGCGAA ATGGCAAGTG 
AAAGACTGTC CGCCAGAGTT GTTGCTCGGA GACTTCAGCA GACGGTTGTC TACTGAAGTC 
ACGCCTGCAG CCATTGCACA GAATAACAGG ACATTTGTGG AGAAACTCCT TAAGGAATGT 
AAGAGCAAAA CAAAACGAAT GCTATTGGAG AAGATGGGTA CAGAAGAGTC TAACTTAGGC 
TTCGGGAGCG AAGTGTCAAT TACTGGTGTC GAGGAAAGCA CTATGATTGC GTCCCTGTGT 
GATTTATTAG AACGACTGTG GTCACATGGC CTACAACACA AAATGGGAAA GTCAGCGCTC 
TGGATGCATC TCATTAACTA CCAAGAGCTG GAACAATGCA GCAATTCAAC TAAACCTATC 
GATCCCAATT ATCTAACACC AGCATTAGCT TGGTGTGTAC TACGGAAACG TCTTGACTAC 
TTATCATCGG TTGGAGCGGA AGTTGAAGCT CAACAAGCAG CCAGTGTGAG TCCGCGATCA 
CGGGACAGCT CCCGCGGCGG ATCCCGCGAT AGCTCTCGGG ACCGTCTGAG TAATTCAGGA 
ACCCTAGAAC GCAAATCGAC TCAGCCCGTT AATCCTTTGC GGCCCTTGCC CGAGAGCTTG 
ACCTTTGATA TGAGGAATGT TCAAGCTATG ACTGATATAA AAACCGAAAT AGGCTACGCT 
CGTGCTTGGG TTCGTTTGTC GCTTGAGAAG AAGCTCCTCT CGAAACATCT CCGTGAATTA 
TTAGCCGATA CCACACTATT GCGCTCTCAA TACAAAAGAA CCGCTTTTCT GCGCTGCGAA 
GAGGAAAGGG AACAATTTTT ATATCACTTA CTGACTTTGA ATGCCGTTGA CTACTATTGC 
TTCACGAACA CGTATCCTAC AACAAAACTT CCATATCGCG TATTAATAAT GCCTTCTCGC 
AAAGCTAGTC AAACGACGGC CAACTGTTGG ATCTCAATCT CTGGTATGAA CGGAGAGTCG 
ACTCCGAAAA TTCCCATACC TCGTAACACA AACGAATTCG TTTTCCATCA CAAGAATCTT 
GGGACATTAT CAACCTTGAG AATCGGTCAC GATAATTCTG GTTTGTCGCC GCGGTGGTTA 
CTCGACCAAG TTTATGTCAG AAATGAAGTG ACAGGACATA CTTACACGTA TCCTTGTAAC 
CGCTGGCTGG GGAGTGGAGT GGACGACGGC TCCACCGAGC GGCTGCTGGT GGCGGCGCGC 
GTGCGCAGCG AGCACACGCC GCGCTCCCCC GCGCCCCCCG CCGCCCTGCA CCCGCACACT 
CCCACCACGC ACCTCTCGAC CTCGACCATC CAGCACATGA TCGGTGATTG CGTGAACGCC 
ATTGTGAAAT GGCACTATCA GTCAAAAAGA GATAAGGACG CGAACTCGCT AAGTGTCTTA 
CTGTGCGGAG AAAATGGCTT GGTCAAGTGC TTGGAGCAAG CTTTCCTCTG TGGCTTCAAA 
TCGGCGCGCT TCTTCGGCAA GAATTTTTTC ATATGGGATT ATTTCGCTAG AGTCAAAGAC 
GAGTTCAAAA TGAGCCTGTG CGACGAGCCC AGTACGATGA ACAACAAGAA CTGCGGCGGC 
GTATCCTACA ACTGCAGACA GGACCAAGAG CACAGAGCCA TCTGGCGATG CTACTGCCAT 
CTCATGGACG AGGTCAACAG TGTGGGCCGC TCGCTGGGGA AGGACGGGAA GTTTCAACTC 
TTCATATGTC TAAGTTTACG GGAACACTGG CTTCATCGAA TGCTGGTGCC TATGTCATTG 
ACTCGTGTCA CAGCTGAGAT GTACGAGGAG CAAAGTTTTC TGCGCAAACA CGGGCTTCTC 
ACGTTTCTGA AACAAATACT CGAGCCTTTG GACGAATTTG ACATTGTACT CGAAAACTCT 
CTCACTCAAG GCATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101744990 ( XM_012691790.1 ) cDNA ORF clone, Bombyx mori(Domestic silkworm) -> XP_012547244.1 Bombyx mori DENN domain-containing protein 5A (LOC101744990), transcript variant X2, mRNA.

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CloneID	OBg19779
Clone ID Related Accession (Same CDS sequence)	XM_012691790.1
Accession Version	XM_012691790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433347200000
Organism	Bombyx mori(Domestic silkworm)
Product	DENN domain-containing protein 5A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004582008.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTCC CTGATTTGTC TGTTAGTTGT CGTTTTGCTG ATTATTTTGC TATATGTGGA 
CTTGATTTCG ATTCTGGATT AGAACCTGAT AGACTATGTG GTGATAATTT ACACGTATCT 
CCTCTCGACC GATCCTATAA AGGAAAAATT TTAGCTCACT ATCCTGAAAA TGTTCCTAGT 
AATCAATTTG ATGAACATGC AACCTGTATG CTCTGTCTAC CAGCTGGCCT CCAATTTAGA 
ACCCAAAAGC ATTCATTGGA GCCCCAATTT CACAGTTTTG TAGTAACTAG AGAAAATGCA 
TCTAGATATT ATGGATTTAG CCTTATATTT TATGAAGAGG TCAGAAATAA AAACATCTGT 
AGTGCTATGC ATACTTTACA GGCGATGTAC ATTACAGAAC TCTCTAGTGG CCAGACTCCA 
CCAGGCCATA GAAAAAGCTC TGGTAAGGAG AGCTCACGAT CCCTCCCCAG ATCGTTCAAA 
CTTACGCCTC ATACTGGTGC TGCTCTAACA TACTTTGATA TAGCCAAAGA TAAACTCTAT 
GTAGCTAAAA GTATAGCACT GATTTGTCAA TACCCCTTTG TAAGAGTTGC CCAAATGTTT 
TTAGAAAACT TGTTCAGATC CTTACCAAGA CAACCAGGAC CTGGTCTTAG CCCAGAATCT 
TATGTTTATA ATTTACTTTA CGAAGTACCA GTGCCCTTGC CAGGTCAAGC TTTAAGACTT 
TATGTGCCAC CTCCTCAACC ACATCTTGAA GCCATTCCAG TTGTGATAAG AATGCCAAAT 
CCTCCAGAAG AGTTACCACT GTTAGACTAT TCGTTGCGTG TCATGTTTTC TAATTTAGGC 
GTTGAACTAG TAGTACAATT GTTCACGTGT GTTCTACTTG AACATCAAGT ATTACTTAGA 
TCAACTGATT ACAACAAGCT GATGCTGGTT TCTGAATGTA TGGTATCGCT ATTGCTTCCC 
TTCACTTGGG CGCACGTCTA CGTACCGATA CTACCGGCTC CTTTATATCA CTTCCTCGAC 
GCGCCCGTAC CATTTGTCAT GGGTTTACAT ACAGACAGCG CGGCCGTTTG CGCCAACGGA 
CCCAGAAGCA TTGCCCTTTG TTCTGAAGCA GCACTGTGTC TGGTAGACAT CGACAAACCC 
GACATACAAC TACCAGAAGA ATTGCCGATA TTCCCACACA GAACATCCTT CATTGAGGAA 
CTCAATCATT TAATGGATAA ATATCAGATT CAGAGACCCG AGACGGAACC AACCAAATTA 
AGTGCGCCCC TAAACGGAAC ATCGTATAGG CACATGAATG ACAACATGAG CAGCAGTTGT 
ACTTTACCAT CAGGCGGCTT ACGCAGGAAG CACTCGTTTC ACGACGTGCT AGAGTGGGAA 
GAAAGTAGGC CGTTGAACGC CTCCCCTCCC GCCTCGCCCA CCCGCCGCGC TCCCCTGCGG 
CCCGACGCCT TGCAGCGTAT CGCCGATATC GTCAAGCGTA CAGGTGTCAA CATTGACGAC 
GTGGATGCCG AAACAGTGAT GCCAACTACT AAAAAGAAAA TTCTCAACGA TGAAGAGCAA 
TATAATGAAG ATATGCGTTT TAACAGTGCT GTTAGAGAAA CGTTTCTCAA TAGATTTGTT 
CATATATTTT TGATGTATGA AAATTTCGTT ATCATGCCCG ATCAGGACCG CGAGTCATGG 
CTGAGCTCAC GCGAATCGAT GGTCAACTTT GACAAGGCGT CCTTCCTCTC CGACCAGCCT 
CAGCGTCACC GACCATTCCT GTCACGCTTC CTCGAAACCC AAATGTTCGC CACGCTCATC 
GACAACAAGA TCATGGCTAA TTGGGGGGAA TATGACGTTA ATTTGCAAGT CTTCGAATAT 
CGGACTAGGA TCGTCAAAAC GAACAGTCTA AAGCGAATAA AAAATGCGAA ATGGCAAGTG 
AAAGACTGTC CGCCAGAGTT GTTGCTCGGA GACTTCAGCA GACGGTTGTC TACTGAAGTC 
ACGCCTGCAG CCATTGCACA GAATAACAGG ACATTTGTGG AGAAACTCCT TAAGGAATGT 
AAGAGCAAAA CAAAACGAAT GCTATTGGAG AAGATGGGTA CAGAAGAGTC TAACTTAGGC 
TTCGGGAGCG AAGTGTCAAT TACTGGTGTC GAGGAAAGCA CTATGATTGC GTCCCTGTGT 
GATTTATTAG AACGACTGTG GTCACATGGC CTACAACACA AAATGGGAAA GTCAGCGCTC 
TGGATGCATC TCATTAACTA CCAAGAGCTG GAACAATGCA GCAATTCAAC TAAACCTATC 
GATCCCAATT ATCTAACACC AGACTTATCA TCGGTTGGAG CGGAAGTTGA AGCTCAACAA 
GCAGCCAGTG TGAGTCCGCG ATCACGGGAC AGCTCCCGCG GCGGATCCCG CGATAGCTCT 
CGGGACCGTC TGAGTAATTC AGGAACCCTA GAACGCAAAT CGACTCAGCC CGTTAATCCT 
TTGCGGCCCT TGCCCGAGAG CTTGACCTTT GATATGAGGA ATGTTCAAGC TATGACTGAT 
ATAAAAACCG AAATAGGCTA CGCTCGTGCT TGGGTTCGTT TGTCGCTTGA GAAGAAGCTC 
CTCTCGAAAC ATCTCCGTGA ATTATTAGCC GATACCACAC TATTGCGCTC TCAATACAAA 
AGAACCGCTT TTCTGCGCTG CGAAGAGGAA AGGGAACAAT TTTTATATCA CTTACTGACT 
TTGAATGCCG TTGACTACTA TTGCTTCACG AACACGTATC CTACAACAAA ACTTCCATAT 
CGCGTATTAA TAATGCCTTC TCGCAAAGCT AGTCAAACGA CGGCCAACTG TTGGATCTCA 
ATCTCTGGTA TGAACGGAGA GTCGACTCCG AAAATTCCCA TACCTCGTAA CACAAACGAA 
TTCGTTTTCC ATCACAAGAA TCTTGGGACA TTATCAACCT TGAGAATCGG TCACGATAAT 
TCTGGTTTGT CGCCGCGGTG GTTACTCGAC CAAGTTTATG TCAGAAATGA AGTGACAGGA 
CATACTTACA CGTATCCTTG TAACCGCTGG CTGGGGAGTG GAGTGGACGA CGGCTCCACC 
GAGCGGCTGC TGGTGGCGGC GCGCGTGCGC AGCGAGCACA CGCCGCGCTC CCCCGCGCCC 
CCCGCCGCCC TGCACCCGCA CACTCCCACC ACGCACCTCT CGACCTCGAC CATCCAGCAC 
ATGATCGGTG ATTGCGTGAA CGCCATTGTG AAATGGCACT ATCAGTCAAA AAGAGATAAG 
GACGCGAACT CGCTAAGTGT CTTACTGTGC GGAGAAAATG GCTTGGTCAA GTGCTTGGAG 
CAAGCTTTCC TCTGTGGCTT CAAATCGGCG CGCTTCTTCG GCAAGAATTT TTTCATATGG 
GATTATTTCG CTAGAGTCAA AGACGAGTTC AAAATGAGCC TGTGCGACGA GCCCAGTACG 
ATGAACAACA AGAACTGCGG CGGCGTATCC TACAACTGCA GACAGGACCA AGAGCACAGA 
GCCATCTGGC GATGCTACTG CCATCTCATG GACGAGGTCA ACAGTGTGGG CCGCTCGCTG 
GGGAAGGACG GGAAGTTTCA ACTCTTCATA TGTCTAAGTT TACGGGAACA CTGGCTTCAT 
CGAATGCTGG TGCCTATGTC ATTGACTCGT GTCACAGCTG AGATGTACGA GGAGCAAAGT 
TTTCTGCGCA AACACGGGCT TCTCACGTTT CTGAAACAAA TACTCGAGCC TTTGGACGAA 
TTTGACATTG TACTCGAAAA CTCTCTCACT CAAGGCATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012547244.1
CDS	219..3980
Translation	MSVPDLSVSCRFADYFAICGLDFDSGLEPDRLCGDNLHVSPLDRSYKGKILAHYPENVPSNQFDEHATCMLCLPAGLQFRTQKHSLEPQFHSFVVTRENASRYYGFSLIFYEEVRNKNICSAMHTLQAMYITELSSGQTPPGHRKSSGKESSRSLPRSFKLTPHTGAALTYFDIAKDKLYVAKSIALICQYPFVRVAQMFLENLFRSLPRQPGPGLSPESYVYNLLYEVPVPLPGQALRLYVPPPQPHLEAIPVVIRMPNPPEELPLLDYSLRVMFSNLGVELVVQLFTCVLLEHQVLLRSTDYNKLMLVSECMVSLLLPFTWAHVYVPILPAPLYHFLDAPVPFVMGLHTDSAAVCANGPRSIALCSEAALCLVDIDKPDIQLPEELPIFPHRTSFIEELNHLMDKYQIQRPETEPTKLSAPLNGTSYRHMNDNMSSSCTLPSGGLRRKHSFHDVLEWEESRPLNASPPASPTRRAPLRPDALQRIADIVKRTGVNIDDVDAETVMPTTKKKILNDEEQYNEDMRFNSAVRETFLNRFVHIFLMYENFVIMPDQDRESWLSSRESMVNFDKASFLSDQPQRHRPFLSRFLETQMFATLIDNKIMANWGEYDVNLQVFEYRTRIVKTNSLKRIKNAKWQVKDCPPELLLGDFSRRLSTEVTPAAIAQNNRTFVEKLLKECKSKTKRMLLEKMGTEESNLGFGSEVSITGVEESTMIASLCDLLERLWSHGLQHKMGKSALWMHLINYQELEQCSNSTKPIDPNYLTPDLSSVGAEVEAQQAASVSPRSRDSSRGGSRDSSRDRLSNSGTLERKSTQPVNPLRPLPESLTFDMRNVQAMTDIKTEIGYARAWVRLSLEKKLLSKHLRELLADTTLLRSQYKRTAFLRCEEEREQFLYHLLTLNAVDYYCFTNTYPTTKLPYRVLIMPSRKASQTTANCWISISGMNGESTPKIPIPRNTNEFVFHHKNLGTLSTLRIGHDNSGLSPRWLLDQVYVRNEVTGHTYTYPCNRWLGSGVDDGSTERLLVAARVRSEHTPRSPAPPAALHPHTPTTHLSTSTIQHMIGDCVNAIVKWHYQSKRDKDANSLSVLLCGENGLVKCLEQAFLCGFKSARFFGKNFFIWDYFARVKDEFKMSLCDEPSTMNNKNCGGVSYNCRQDQEHRAIWRCYCHLMDEVNSVGRSLGKDGKFQLFICLSLREHWLHRMLVPMSLTRVTAEMYEEQSFLRKHGLLTFLKQILEPLDEFDIVLENSLTQGI

Target ORF information:

RefSeq Version	XM_012691790.1
Organism	Bombyx mori(Domestic silkworm)
Definition	Bombyx mori DENN domain-containing protein 5A (LOC101744990), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012691790.1

ORF Insert Sequence:

ATGTCTGTCC CTGATTTGTC TGTTAGTTGT CGTTTTGCTG ATTATTTTGC TATATGTGGA 
CTTGATTTCG ATTCTGGATT AGAACCTGAT AGACTATGTG GTGATAATTT ACACGTATCT 
CCTCTCGACC GATCCTATAA AGGAAAAATT TTAGCTCACT ATCCTGAAAA TGTTCCTAGT 
AATCAATTTG ATGAACATGC AACCTGTATG CTCTGTCTAC CAGCTGGCCT CCAATTTAGA 
ACCCAAAAGC ATTCATTGGA GCCCCAATTT CACAGTTTTG TAGTAACTAG AGAAAATGCA 
TCTAGATATT ATGGATTTAG CCTTATATTT TATGAAGAGG TCAGAAATAA AAACATCTGT 
AGTGCTATGC ATACTTTACA GGCGATGTAC ATTACAGAAC TCTCTAGTGG CCAGACTCCA 
CCAGGCCATA GAAAAAGCTC TGGTAAGGAG AGCTCACGAT CCCTCCCCAG ATCGTTCAAA 
CTTACGCCTC ATACTGGTGC TGCTCTAACA TACTTTGATA TAGCCAAAGA TAAACTCTAT 
GTAGCTAAAA GTATAGCACT GATTTGTCAA TACCCCTTTG TAAGAGTTGC CCAAATGTTT 
TTAGAAAACT TGTTCAGATC CTTACCAAGA CAACCAGGAC CTGGTCTTAG CCCAGAATCT 
TATGTTTATA ATTTACTTTA CGAAGTACCA GTGCCCTTGC CAGGTCAAGC TTTAAGACTT 
TATGTGCCAC CTCCTCAACC ACATCTTGAA GCCATTCCAG TTGTGATAAG AATGCCAAAT 
CCTCCAGAAG AGTTACCACT GTTAGACTAT TCGTTGCGTG TCATGTTTTC TAATTTAGGC 
GTTGAACTAG TAGTACAATT GTTCACGTGT GTTCTACTTG AACATCAAGT ATTACTTAGA 
TCAACTGATT ACAACAAGCT GATGCTGGTT TCTGAATGTA TGGTATCGCT ATTGCTTCCC 
TTCACTTGGG CGCACGTCTA CGTACCGATA CTACCGGCTC CTTTATATCA CTTCCTCGAC 
GCGCCCGTAC CATTTGTCAT GGGTTTACAT ACAGACAGCG CGGCCGTTTG CGCCAACGGA 
CCCAGAAGCA TTGCCCTTTG TTCTGAAGCA GCACTGTGTC TGGTAGACAT CGACAAACCC 
GACATACAAC TACCAGAAGA ATTGCCGATA TTCCCACACA GAACATCCTT CATTGAGGAA 
CTCAATCATT TAATGGATAA ATATCAGATT CAGAGACCCG AGACGGAACC AACCAAATTA 
AGTGCGCCCC TAAACGGAAC ATCGTATAGG CACATGAATG ACAACATGAG CAGCAGTTGT 
ACTTTACCAT CAGGCGGCTT ACGCAGGAAG CACTCGTTTC ACGACGTGCT AGAGTGGGAA 
GAAAGTAGGC CGTTGAACGC CTCCCCTCCC GCCTCGCCCA CCCGCCGCGC TCCCCTGCGG 
CCCGACGCCT TGCAGCGTAT CGCCGATATC GTCAAGCGTA CAGGTGTCAA CATTGACGAC 
GTGGATGCCG AAACAGTGAT GCCAACTACT AAAAAGAAAA TTCTCAACGA TGAAGAGCAA 
TATAATGAAG ATATGCGTTT TAACAGTGCT GTTAGAGAAA CGTTTCTCAA TAGATTTGTT 
CATATATTTT TGATGTATGA AAATTTCGTT ATCATGCCCG ATCAGGACCG CGAGTCATGG 
CTGAGCTCAC GCGAATCGAT GGTCAACTTT GACAAGGCGT CCTTCCTCTC CGACCAGCCT 
CAGCGTCACC GACCATTCCT GTCACGCTTC CTCGAAACCC AAATGTTCGC CACGCTCATC 
GACAACAAGA TCATGGCTAA TTGGGGGGAA TATGACGTTA ATTTGCAAGT CTTCGAATAT 
CGGACTAGGA TCGTCAAAAC GAACAGTCTA AAGCGAATAA AAAATGCGAA ATGGCAAGTG 
AAAGACTGTC CGCCAGAGTT GTTGCTCGGA GACTTCAGCA GACGGTTGTC TACTGAAGTC 
ACGCCTGCAG CCATTGCACA GAATAACAGG ACATTTGTGG AGAAACTCCT TAAGGAATGT 
AAGAGCAAAA CAAAACGAAT GCTATTGGAG AAGATGGGTA CAGAAGAGTC TAACTTAGGC 
TTCGGGAGCG AAGTGTCAAT TACTGGTGTC GAGGAAAGCA CTATGATTGC GTCCCTGTGT 
GATTTATTAG AACGACTGTG GTCACATGGC CTACAACACA AAATGGGAAA GTCAGCGCTC 
TGGATGCATC TCATTAACTA CCAAGAGCTG GAACAATGCA GCAATTCAAC TAAACCTATC 
GATCCCAATT ATCTAACACC AGACTTATCA TCGGTTGGAG CGGAAGTTGA AGCTCAACAA 
GCAGCCAGTG TGAGTCCGCG ATCACGGGAC AGCTCCCGCG GCGGATCCCG CGATAGCTCT 
CGGGACCGTC TGAGTAATTC AGGAACCCTA GAACGCAAAT CGACTCAGCC CGTTAATCCT 
TTGCGGCCCT TGCCCGAGAG CTTGACCTTT GATATGAGGA ATGTTCAAGC TATGACTGAT 
ATAAAAACCG AAATAGGCTA CGCTCGTGCT TGGGTTCGTT TGTCGCTTGA GAAGAAGCTC 
CTCTCGAAAC ATCTCCGTGA ATTATTAGCC GATACCACAC TATTGCGCTC TCAATACAAA 
AGAACCGCTT TTCTGCGCTG CGAAGAGGAA AGGGAACAAT TTTTATATCA CTTACTGACT 
TTGAATGCCG TTGACTACTA TTGCTTCACG AACACGTATC CTACAACAAA ACTTCCATAT 
CGCGTATTAA TAATGCCTTC TCGCAAAGCT AGTCAAACGA CGGCCAACTG TTGGATCTCA 
ATCTCTGGTA TGAACGGAGA GTCGACTCCG AAAATTCCCA TACCTCGTAA CACAAACGAA 
TTCGTTTTCC ATCACAAGAA TCTTGGGACA TTATCAACCT TGAGAATCGG TCACGATAAT 
TCTGGTTTGT CGCCGCGGTG GTTACTCGAC CAAGTTTATG TCAGAAATGA AGTGACAGGA 
CATACTTACA CGTATCCTTG TAACCGCTGG CTGGGGAGTG GAGTGGACGA CGGCTCCACC 
GAGCGGCTGC TGGTGGCGGC GCGCGTGCGC AGCGAGCACA CGCCGCGCTC CCCCGCGCCC 
CCCGCCGCCC TGCACCCGCA CACTCCCACC ACGCACCTCT CGACCTCGAC CATCCAGCAC 
ATGATCGGTG ATTGCGTGAA CGCCATTGTG AAATGGCACT ATCAGTCAAA AAGAGATAAG 
GACGCGAACT CGCTAAGTGT CTTACTGTGC GGAGAAAATG GCTTGGTCAA GTGCTTGGAG 
CAAGCTTTCC TCTGTGGCTT CAAATCGGCG CGCTTCTTCG GCAAGAATTT TTTCATATGG 
GATTATTTCG CTAGAGTCAA AGACGAGTTC AAAATGAGCC TGTGCGACGA GCCCAGTACG 
ATGAACAACA AGAACTGCGG CGGCGTATCC TACAACTGCA GACAGGACCA AGAGCACAGA 
GCCATCTGGC GATGCTACTG CCATCTCATG GACGAGGTCA ACAGTGTGGG CCGCTCGCTG 
GGGAAGGACG GGAAGTTTCA ACTCTTCATA TGTCTAAGTT TACGGGAACA CTGGCTTCAT 
CGAATGCTGG TGCCTATGTC ATTGACTCGT GTCACAGCTG AGATGTACGA GGAGCAAAGT 
TTTCTGCGCA AACACGGGCT TCTCACGTTT CTGAAACAAA TACTCGAGCC TTTGGACGAA 
TTTGACATTG TACTCGAAAA CTCTCTCACT CAAGGCATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101744990 ( XM_012691791.1 ) cDNA ORF clone, Bombyx mori(Domestic silkworm) -> XP_012547245.1 Bombyx mori DENN domain-containing protein 5A (LOC101744990), transcript variant X3, mRNA.

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CloneID	OBg19780
Clone ID Related Accession (Same CDS sequence)	XM_012691791.1
Accession Version	XM_012691791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433347200000
Organism	Bombyx mori(Domestic silkworm)
Product	DENN domain-containing protein 5B isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004582008.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTCC CTGATTTGTC TGTTAGTTGT CGTTTTGCTG ATTATTTTGC TATATGTGGA 
CTTGATTTCG ATTCTGGATT AGAACCTGAT AGACTATGTG GTGATAATTT ACACGTATCT 
CCTCTCGACC GATCCTATAA AGGAAAAATT TTAGCTCACT ATCCTGAAAA TGTTCCTAGT 
AATCAATTTG ATGAACATGC AACCTGTATG CTCTGTCTAC CAGCTGGCCT CCAATTTAGA 
ACCCAAAAGC ATTCATTGGA GCCCCAATTT CACAGTTTTG TAGTAACTAG AGAAAATGCA 
TCTAGATATT ATGGATTTAG CCTTATATTT TATGAAGAGG TCAGAAATAA AAACATCTGT 
AGTGCTATGC ATACTTTACA GGCGATGTAC ATTACAGAAC TCTCTAGTGG CCAGACTCCA 
CCAGGCCATA GAAAAAGCTC TGGTAAGGAG AGCTCACGAT CCCTCCCCAG ATCGTTCAAA 
CTTACGCCTC ATACTGGTGC TGCTCTAACA TACTTTGATA TAGCCAAAGA TAAACTCTAT 
GTAGCTAAAA GTATAGCACT GATTTGTCAA TACCCCTTTG TAAGAGTTGC CCAAATGTTT 
TTAGAAAACT TGTTCAGATC CTTACCAAGA CAACCAGGAC CTGGTCTTAG CCCAGAATCT 
TATGTTTATA ATTTACTTTA CGAAGTACCA GTGCCCTTGC CAGGTCAAGC TTTAAGACTT 
TATGTGCCAC CTCCTCAACC ACATCTTGAA GCCATTCCAG TTGTGATAAG AATGCCAAAT 
CCTCCAGAAG AGTTACCACT GTTAGACTAT TCGTTGCGTG TCATGTTTTC TAATTTAGGC 
GTTGAACTAG TAGTACAATT GTTCACGTGT GTTCTACTTG AACATCAAGT ATTACTTAGA 
TCAACTGATT ACAACAAGCT GATGCTGGTT TCTGAATGTA TGGTATCGCT ATTGCTTCCC 
TTCACTTGGG CGCACGTCTA CGTACCGATA CTACCGGCTC CTTTATATCA CTTCCTCGAC 
GCGCCCGTAC CATTTGTCAT GGGTTTACAT ACAGACAGCG CGGCCGTTTG CGCCAACGGA 
CCCAGAAGCA TTGCCCTTTG TTCTGAAGCA GCACTGTGTC TGGTAGACAT CGACAAACCC 
GACATACAAC TACCAGAAGA ATTGCCGATA TTCCCACACA GAACATCCTT CATTGAGGAA 
CTCAATCATT TAATGGATAA ATATCAGATT CAGAGACCCG AGACGGAACC AACCAAATTA 
AGTGCGCCCC TAAACGGAAC ATCGTATAGG CACATGAATG ACAACATGAG CAGCAGTTGT 
ACTTTACCAT CAGGTGTCAA CATTGACGAC GTGGATGCCG AAACAGTGAT GCCAACTACT 
AAAAAGAAAA TTCTCAACGA TGAAGAGCAA TATAATGAAG ATATGCGTTT TAACAGTGCT 
GTTAGAGAAA CGTTTCTCAA TAGATTTGTT CATATATTTT TGATGTATGA AAATTTCGTT 
ATCATGCCCG ATCAGGACCG CGAGTCATGG CTGAGCTCAC GCGAATCGAT GGTCAACTTT 
GACAAGGCGT CCTTCCTCTC CGACCAGCCT CAGCGTCACC GACCATTCCT GTCACGCTTC 
CTCGAAACCC AAATGTTCGC CACGCTCATC GACAACAAGA TCATGGCTAA TTGGGGGGAA 
TATGACGTTA ATTTGCAAGT CTTCGAATAT CGGACTAGGA TCGTCAAAAC GAACAGTCTA 
AAGCGAATAA AAAATGCGAA ATGGCAAGTG AAAGACTGTC CGCCAGAGTT GTTGCTCGGA 
GACTTCAGCA GACGGTTGTC TACTGAAGTC ACGCCTGCAG CCATTGCACA GAATAACAGG 
ACATTTGTGG AGAAACTCCT TAAGGAATGT AAGAGCAAAA CAAAACGAAT GCTATTGGAG 
AAGATGGGTA CAGAAGAGTC TAACTTAGGC TTCGGGAGCG AAGTGTCAAT TACTGGTGTC 
GAGGAAAGCA CTATGATTGC GTCCCTGTGT GATTTATTAG AACGACTGTG GTCACATGGC 
CTACAACACA AAATGGGAAA GTCAGCGCTC TGGATGCATC TCATTAACTA CCAAGAGCTG 
GAACAATGCA GCAATTCAAC TAAACCTATC GATCCCAATT ATCTAACACC AGCATTAGCT 
TGGTGTGTAC TACGGAAACG TCTTGACTAC TTATCATCGG TTGGAGCGGA AGTTGAAGCT 
CAACAAGCAG CCAGTGTGAG TCCGCGATCA CGGGACAGCT CCCGCGGCGG ATCCCGCGAT 
AGCTCTCGGG ACCGTCTGAG TAATTCAGGA ACCCTAGAAC GCAAATCGAC TCAGCCCGTT 
AATCCTTTGC GGCCCTTGCC CGAGAGCTTG ACCTTTGATA TGAGGAATGT TCAAGCTATG 
ACTGATATAA AAACCGAAAT AGGCTACGCT CGTGCTTGGG TTCGTTTGTC GCTTGAGAAG 
AAGCTCCTCT CGAAACATCT CCGTGAATTA TTAGCCGATA CCACACTATT GCGCTCTCAA 
TACAAAAGAA CCGCTTTTCT GCGCTGCGAA GAGGAAAGGG AACAATTTTT ATATCACTTA 
CTGACTTTGA ATGCCGTTGA CTACTATTGC TTCACGAACA CGTATCCTAC AACAAAACTT 
CCATATCGCG TATTAATAAT GCCTTCTCGC AAAGCTAGTC AAACGACGGC CAACTGTTGG 
ATCTCAATCT CTGGTATGAA CGGAGAGTCG ACTCCGAAAA TTCCCATACC TCGTAACACA 
AACGAATTCG TTTTCCATCA CAAGAATCTT GGGACATTAT CAACCTTGAG AATCGGTCAC 
GATAATTCTG GTTTGTCGCC GCGGTGGTTA CTCGACCAAG TTTATGTCAG AAATGAAGTG 
ACAGGACATA CTTACACGTA TCCTTGTAAC CGCTGGCTGG GGAGTGGAGT GGACGACGGC 
TCCACCGAGC GGCTGCTGGT GGCGGCGCGC GTGCGCAGCG AGCACACGCC GCGCTCCCCC 
GCGCCCCCCG CCGCCCTGCA CCCGCACACT CCCACCACGC ACCTCTCGAC CTCGACCATC 
CAGCACATGA TCGGTGATTG CGTGAACGCC ATTGTGAAAT GGCACTATCA GTCAAAAAGA 
GATAAGGACG CGAACTCGCT AAGTGTCTTA CTGTGCGGAG AAAATGGCTT GGTCAAGTGC 
TTGGAGCAAG CTTTCCTCTG TGGCTTCAAA TCGGCGCGCT TCTTCGGCAA GAATTTTTTC 
ATATGGGATT ATTTCGCTAG AGTCAAAGAC GAGTTCAAAA TGAGCCTGTG CGACGAGCCC 
AGTACGATGA ACAACAAGAA CTGCGGCGGC GTATCCTACA ACTGCAGACA GGACCAAGAG 
CACAGAGCCA TCTGGCGATG CTACTGCCAT CTCATGGACG AGGTCAACAG TGTGGGCCGC 
TCGCTGGGGA AGGACGGGAA GTTTCAACTC TTCATATGTC TAAGTTTACG GGAACACTGG 
CTTCATCGAA TGCTGGTGCC TATGTCATTG ACTCGTGTCA CAGCTGAGAT GTACGAGGAG 
CAAAGTTTTC TGCGCAAACA CGGGCTTCTC ACGTTTCTGA AACAAATACT CGAGCCTTTG 
GACGAATTTG ACATTGTACT CGAAAACTCT CTCACTCAAG GCATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012547245.1
CDS	219..3866
Translation	MSVPDLSVSCRFADYFAICGLDFDSGLEPDRLCGDNLHVSPLDRSYKGKILAHYPENVPSNQFDEHATCMLCLPAGLQFRTQKHSLEPQFHSFVVTRENASRYYGFSLIFYEEVRNKNICSAMHTLQAMYITELSSGQTPPGHRKSSGKESSRSLPRSFKLTPHTGAALTYFDIAKDKLYVAKSIALICQYPFVRVAQMFLENLFRSLPRQPGPGLSPESYVYNLLYEVPVPLPGQALRLYVPPPQPHLEAIPVVIRMPNPPEELPLLDYSLRVMFSNLGVELVVQLFTCVLLEHQVLLRSTDYNKLMLVSECMVSLLLPFTWAHVYVPILPAPLYHFLDAPVPFVMGLHTDSAAVCANGPRSIALCSEAALCLVDIDKPDIQLPEELPIFPHRTSFIEELNHLMDKYQIQRPETEPTKLSAPLNGTSYRHMNDNMSSSCTLPSGVNIDDVDAETVMPTTKKKILNDEEQYNEDMRFNSAVRETFLNRFVHIFLMYENFVIMPDQDRESWLSSRESMVNFDKASFLSDQPQRHRPFLSRFLETQMFATLIDNKIMANWGEYDVNLQVFEYRTRIVKTNSLKRIKNAKWQVKDCPPELLLGDFSRRLSTEVTPAAIAQNNRTFVEKLLKECKSKTKRMLLEKMGTEESNLGFGSEVSITGVEESTMIASLCDLLERLWSHGLQHKMGKSALWMHLINYQELEQCSNSTKPIDPNYLTPALAWCVLRKRLDYLSSVGAEVEAQQAASVSPRSRDSSRGGSRDSSRDRLSNSGTLERKSTQPVNPLRPLPESLTFDMRNVQAMTDIKTEIGYARAWVRLSLEKKLLSKHLRELLADTTLLRSQYKRTAFLRCEEEREQFLYHLLTLNAVDYYCFTNTYPTTKLPYRVLIMPSRKASQTTANCWISISGMNGESTPKIPIPRNTNEFVFHHKNLGTLSTLRIGHDNSGLSPRWLLDQVYVRNEVTGHTYTYPCNRWLGSGVDDGSTERLLVAARVRSEHTPRSPAPPAALHPHTPTTHLSTSTIQHMIGDCVNAIVKWHYQSKRDKDANSLSVLLCGENGLVKCLEQAFLCGFKSARFFGKNFFIWDYFARVKDEFKMSLCDEPSTMNNKNCGGVSYNCRQDQEHRAIWRCYCHLMDEVNSVGRSLGKDGKFQLFICLSLREHWLHRMLVPMSLTRVTAEMYEEQSFLRKHGLLTFLKQILEPLDEFDIVLENSLTQGI

Target ORF information:

RefSeq Version	XM_012691791.1
Organism	Bombyx mori(Domestic silkworm)
Definition	Bombyx mori DENN domain-containing protein 5A (LOC101744990), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012691791.1

ORF Insert Sequence:

ATGTCTGTCC CTGATTTGTC TGTTAGTTGT CGTTTTGCTG ATTATTTTGC TATATGTGGA 
CTTGATTTCG ATTCTGGATT AGAACCTGAT AGACTATGTG GTGATAATTT ACACGTATCT 
CCTCTCGACC GATCCTATAA AGGAAAAATT TTAGCTCACT ATCCTGAAAA TGTTCCTAGT 
AATCAATTTG ATGAACATGC AACCTGTATG CTCTGTCTAC CAGCTGGCCT CCAATTTAGA 
ACCCAAAAGC ATTCATTGGA GCCCCAATTT CACAGTTTTG TAGTAACTAG AGAAAATGCA 
TCTAGATATT ATGGATTTAG CCTTATATTT TATGAAGAGG TCAGAAATAA AAACATCTGT 
AGTGCTATGC ATACTTTACA GGCGATGTAC ATTACAGAAC TCTCTAGTGG CCAGACTCCA 
CCAGGCCATA GAAAAAGCTC TGGTAAGGAG AGCTCACGAT CCCTCCCCAG ATCGTTCAAA 
CTTACGCCTC ATACTGGTGC TGCTCTAACA TACTTTGATA TAGCCAAAGA TAAACTCTAT 
GTAGCTAAAA GTATAGCACT GATTTGTCAA TACCCCTTTG TAAGAGTTGC CCAAATGTTT 
TTAGAAAACT TGTTCAGATC CTTACCAAGA CAACCAGGAC CTGGTCTTAG CCCAGAATCT 
TATGTTTATA ATTTACTTTA CGAAGTACCA GTGCCCTTGC CAGGTCAAGC TTTAAGACTT 
TATGTGCCAC CTCCTCAACC ACATCTTGAA GCCATTCCAG TTGTGATAAG AATGCCAAAT 
CCTCCAGAAG AGTTACCACT GTTAGACTAT TCGTTGCGTG TCATGTTTTC TAATTTAGGC 
GTTGAACTAG TAGTACAATT GTTCACGTGT GTTCTACTTG AACATCAAGT ATTACTTAGA 
TCAACTGATT ACAACAAGCT GATGCTGGTT TCTGAATGTA TGGTATCGCT ATTGCTTCCC 
TTCACTTGGG CGCACGTCTA CGTACCGATA CTACCGGCTC CTTTATATCA CTTCCTCGAC 
GCGCCCGTAC CATTTGTCAT GGGTTTACAT ACAGACAGCG CGGCCGTTTG CGCCAACGGA 
CCCAGAAGCA TTGCCCTTTG TTCTGAAGCA GCACTGTGTC TGGTAGACAT CGACAAACCC 
GACATACAAC TACCAGAAGA ATTGCCGATA TTCCCACACA GAACATCCTT CATTGAGGAA 
CTCAATCATT TAATGGATAA ATATCAGATT CAGAGACCCG AGACGGAACC AACCAAATTA 
AGTGCGCCCC TAAACGGAAC ATCGTATAGG CACATGAATG ACAACATGAG CAGCAGTTGT 
ACTTTACCAT CAGGTGTCAA CATTGACGAC GTGGATGCCG AAACAGTGAT GCCAACTACT 
AAAAAGAAAA TTCTCAACGA TGAAGAGCAA TATAATGAAG ATATGCGTTT TAACAGTGCT 
GTTAGAGAAA CGTTTCTCAA TAGATTTGTT CATATATTTT TGATGTATGA AAATTTCGTT 
ATCATGCCCG ATCAGGACCG CGAGTCATGG CTGAGCTCAC GCGAATCGAT GGTCAACTTT 
GACAAGGCGT CCTTCCTCTC CGACCAGCCT CAGCGTCACC GACCATTCCT GTCACGCTTC 
CTCGAAACCC AAATGTTCGC CACGCTCATC GACAACAAGA TCATGGCTAA TTGGGGGGAA 
TATGACGTTA ATTTGCAAGT CTTCGAATAT CGGACTAGGA TCGTCAAAAC GAACAGTCTA 
AAGCGAATAA AAAATGCGAA ATGGCAAGTG AAAGACTGTC CGCCAGAGTT GTTGCTCGGA 
GACTTCAGCA GACGGTTGTC TACTGAAGTC ACGCCTGCAG CCATTGCACA GAATAACAGG 
ACATTTGTGG AGAAACTCCT TAAGGAATGT AAGAGCAAAA CAAAACGAAT GCTATTGGAG 
AAGATGGGTA CAGAAGAGTC TAACTTAGGC TTCGGGAGCG AAGTGTCAAT TACTGGTGTC 
GAGGAAAGCA CTATGATTGC GTCCCTGTGT GATTTATTAG AACGACTGTG GTCACATGGC 
CTACAACACA AAATGGGAAA GTCAGCGCTC TGGATGCATC TCATTAACTA CCAAGAGCTG 
GAACAATGCA GCAATTCAAC TAAACCTATC GATCCCAATT ATCTAACACC AGCATTAGCT 
TGGTGTGTAC TACGGAAACG TCTTGACTAC TTATCATCGG TTGGAGCGGA AGTTGAAGCT 
CAACAAGCAG CCAGTGTGAG TCCGCGATCA CGGGACAGCT CCCGCGGCGG ATCCCGCGAT 
AGCTCTCGGG ACCGTCTGAG TAATTCAGGA ACCCTAGAAC GCAAATCGAC TCAGCCCGTT 
AATCCTTTGC GGCCCTTGCC CGAGAGCTTG ACCTTTGATA TGAGGAATGT TCAAGCTATG 
ACTGATATAA AAACCGAAAT AGGCTACGCT CGTGCTTGGG TTCGTTTGTC GCTTGAGAAG 
AAGCTCCTCT CGAAACATCT CCGTGAATTA TTAGCCGATA CCACACTATT GCGCTCTCAA 
TACAAAAGAA CCGCTTTTCT GCGCTGCGAA GAGGAAAGGG AACAATTTTT ATATCACTTA 
CTGACTTTGA ATGCCGTTGA CTACTATTGC TTCACGAACA CGTATCCTAC AACAAAACTT 
CCATATCGCG TATTAATAAT GCCTTCTCGC AAAGCTAGTC AAACGACGGC CAACTGTTGG 
ATCTCAATCT CTGGTATGAA CGGAGAGTCG ACTCCGAAAA TTCCCATACC TCGTAACACA 
AACGAATTCG TTTTCCATCA CAAGAATCTT GGGACATTAT CAACCTTGAG AATCGGTCAC 
GATAATTCTG GTTTGTCGCC GCGGTGGTTA CTCGACCAAG TTTATGTCAG AAATGAAGTG 
ACAGGACATA CTTACACGTA TCCTTGTAAC CGCTGGCTGG GGAGTGGAGT GGACGACGGC 
TCCACCGAGC GGCTGCTGGT GGCGGCGCGC GTGCGCAGCG AGCACACGCC GCGCTCCCCC 
GCGCCCCCCG CCGCCCTGCA CCCGCACACT CCCACCACGC ACCTCTCGAC CTCGACCATC 
CAGCACATGA TCGGTGATTG CGTGAACGCC ATTGTGAAAT GGCACTATCA GTCAAAAAGA 
GATAAGGACG CGAACTCGCT AAGTGTCTTA CTGTGCGGAG AAAATGGCTT GGTCAAGTGC 
TTGGAGCAAG CTTTCCTCTG TGGCTTCAAA TCGGCGCGCT TCTTCGGCAA GAATTTTTTC 
ATATGGGATT ATTTCGCTAG AGTCAAAGAC GAGTTCAAAA TGAGCCTGTG CGACGAGCCC 
AGTACGATGA ACAACAAGAA CTGCGGCGGC GTATCCTACA ACTGCAGACA GGACCAAGAG 
CACAGAGCCA TCTGGCGATG CTACTGCCAT CTCATGGACG AGGTCAACAG TGTGGGCCGC 
TCGCTGGGGA AGGACGGGAA GTTTCAACTC TTCATATGTC TAAGTTTACG GGAACACTGG 
CTTCATCGAA TGCTGGTGCC TATGTCATTG ACTCGTGTCA CAGCTGAGAT GTACGAGGAG 
CAAAGTTTTC TGCGCAAACA CGGGCTTCTC ACGTTTCTGA AACAAATACT CGAGCCTTTG 
GACGAATTTG ACATTGTACT CGAAAACTCT CTCACTCAAG GCATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101744990 cDNA ORF clone, Bombyx mori(Domestic silkworm)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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