NOS2 cDNA ORF clone, Bombyx mori(domestic silkworm)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOS2
Entrez Gene ID	100144542
Full Name	nitric oxide synthase 2
Synonyms	BmNOS2,Nsl
Gene Type	protein-coding
Organism	Bombyx mori(domestic silkworm)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021347365.1	XP_021203040.1	nitric oxide synthase-like protein isoform X2
XM_012689821.1	XP_012545275.1	nitric oxide synthase-like protein isoform X1
XM_012689821.2	XP_012545275.1	nitric oxide synthase-like protein isoform X1
XM_021347397.1	XP_021203072.1	nitric oxide synthase-like protein isoform X2
NM_001123336.1	NP_001116808.1	nitric oxide synthase-like protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Success in the acquisition of Bombyx mori sperm motility is influenced by the extracellular production of nitric oxide (NO) in the presence of seminal fluid nitric oxide synthase (NOS).
Nagaoka S, Asagoshi M, Kato K, Takata Y
Insect biochemistry and molecular biology8440-47(2017 05)

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
Ou J, Deng HM, Zheng SC, Huang LH, Feng QL, Liu L
BMC genomics15820(2014 Sep)

Large scale full-length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori.
Suetsugu Y, Futahashi R, Kanamori H, Kadono-Okuda K, Sasanuma S, Narukawa J, Ajimura M, Jouraku A, Namiki N, Shimomura M, Sezutsu H, Osanai-Futahashi M, Suzuki MG, Daimon T, Shinoda T, Taniai K, Asaoka K, Niwa R, Kawaoka S, Katsuma S, Tamura T, Noda H, Kasahara M, Sugano S, Suzuki Y, Fujiwara H, Kataoka H, Arunkumar KP, Tomar A, Nagaraju J, Goldsmith MR, Feng Q, Xia Q, Yamamoto K, Shimada T, Mita K
G3 (Bethesda, Md.)3(9)1481-92(2013 Sep)

A genome-wide analysis of genes and gene families involved in innate immunity of Bombyx mori.
Tanaka H, Ishibashi J, Fujita K, Nakajima Y, Sagisaka A, Tomimoto K, Suzuki N, Yoshiyama M, Kaneko Y, Iwasaki T, Sunagawa T, Yamaji K, Asaoka A, Mita K, Yamakawa M
Insect biochemistry and molecular biology38(12)1087-110(2008 Dec)

Positional cloning of a Bombyx wingless locus flugellos (fl) reveals a crucial role for fringe that is specific for wing morphogenesis.
Sato K, Matsunaga TM, Futahashi R, Kojima T, Mita K, Banno Y, Fujiwara H
Genetics179(2)875-85(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NOS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBg13837	NM_001123336.1 Latest version!	Bombyx mori nitric oxide synthase 2 (NOS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBg14309	XM_012689821.2 Latest version!	Bombyx mori nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBg22348	XM_021347397.1 Latest version!	Bombyx mori nitric oxide synthase 2 (NOS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBg22348	XM_021347365.1 Latest version!	Bombyx mori nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OBg14309	XM_012689821.1	Bombyx mori nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOS2 ( NM_001123336.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> NP_001116808.1 Bombyx mori nitric oxide synthase 2 (NOS2), mRNA.

Price & Availability↑

CloneID	OBg13837
Clone ID Related Accession (Same CDS sequence)	NM_001123336.1
Accession Version	NM_001123336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Bombyx mori(domestic silkworm)
Product	nitric oxide synthase-like protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB360590.1. ##Evidence-Data-START## Transcript exon combination :: AB360590.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03349348, SAMN03699702 [ECO:0000348] ##Evidence-Data-END##

ATGGCCATCC CGGGACGAGG CGACGTGCCC CGAGACCCGG AGGAGGTGCT TAATGACGCA 
AAGGACTTTC TAGGCCAGTA CTTCGCTTCT ATACGGAGGG CCAATACTCC AGCTCATGAA 
GCTCGCTGGA AAATCGTACA AGAGGAAGTA GCTACGACTG GCACATACCA ACTAACCACA 
ACAGAACTGG TATTTGGAGC CAAGCTTGCA TGGAGAAACG CTAGCCGTTG CATTGGCAGA 
ATCCAGTGGT CAAAATTGCA GGTATTCGAT TGTCGCCAAG TCACAACAAC TAGTGGAATG 
TTCGAAGCAC TCTGTAACCA CATCAAGTAC AGCACAAATA AAGGAAATAT AAGATCAGCT 
ATAACATTGT TCCCACAACG TACGGACGGC AAGCACGACT ACAGAATATG GAACAGCCAG 
CTGATCAGCT ACGCTGGCTA CCGTCAGCCA GACGGTACGG TGCTTGGTGA CCCCATGCAC 
TGCGAATTCA CTGATCTCTG CTTGAAACTT GGCTGGAAGC CGCCACGCAC GGCCTGGGAT 
ATTCTTCCCC TAGTTCTGTC AGCTAACGGG AAAGACCCTG ACTACTTCGA AATCCCCAGG 
GAGCTTGTTA TGGAAATCCA CATGACGCAC CCCACGTTCG AATGGTTCAA AGAACTAGAA 
TTACGTTGGT ACGCTTTGCC CGCCGTCTCC AGTATGCGTT TTGATTGCGG TGGCATCGAA 
TTCACGGCCA ACGCTTTCAA CGGTTGGTAC ATGAGCACTG AAATCGGCTG CAGGAACTTC 
TGTGACACCA ACCGGCTTAA TGTTTTGGAG AAAATAGCTC AAAACATGGG CCTAGATACC 
AGAACTCCGG TCAATCTTTG GAAGGATAAG GCCCTCGTGG AAGTGAACGT GGCTGTACTG 
CACAGCTTTC AACAGCACAA CGCGACTATC GTGGATCATC ATACAGCTTC TGAAAGCTTC 
ATAAAGCATT TGGATAACGA GAACCGGCTT CGGTCTGGAT GTCCAGCCGA TTGGATATGG 
ATCGTACCTC CCATGTCATC GTCCATAACC CCGGTGTTTC ACCAAGAAAT GGCGCTCTAC 
TACTTGAAAC CTTCATACGA ATATCAGGAG CCTGCATGGA AGACGCATCA GTGGCAGAAA 
TCTAAGCCGA TCACAGGCAA AAGACCCATA AACAGAAAGT TCCATTTCAA GCAAATTGCC 
CGAGCTGTCA AATTCACTTC GAAATTGTTC GGTCGTGCCC TTTCGAAGAG GATTAAAGCG 
ACTGTGCTGT ACGCTACGGA GACTGGGAAA TCGGAACAAT ACGCCAAAGA GCTTGGTGTT 
ATATTTGGAC ACGCCTTTAA TGCGCAGGTT CATTGTATGG CGGATTACGA TATAACTTCA 
ATTGAACACG AAGCTCTCCT TCTGGTTGTG ACATCGACGT TTGGAAATGG TGACCCACCC 
GAGAATGGTG TCGCATTTGG AGAGCATCTA TGCGAAATAT TATACGCTGA TCAAGTCGGT 
GAAGATTCTA CTGGAAATCA AATGTTGACA CCAAAATCGT TCATAAAAGC CAATAGTGAC 
ATTCAAAGGT ATACAGCAGG AAATCCGAAA AAACTCAACA GACTCGAATC ATTGAAGGGT 
AGTACAACAG ACGCCACATC CATTGACAGC TTTGGTCCAT TGAGTAATGT TAGATTTGCC 
GTGTTCGCTC TGGGTTCGAG CGCTTACCCG AATTTCTGTA ACTTCGGAAA GTATGTAGAC 
AAATTGTTGG TTGACCTCGG CGGGGAACGA ATCCACGACC TCGCAACCGG TGACGAAATG 
TGCGGCCAAG ATCAGGCCTT CCGGAAGTGG GCCTCTAGCG TCTTCAACGT TGCTTGTGAG 
ACATTCTGCT TGGATGATGA TGAGACTCTA CAAGAGGCAA AGAGAGCTCT CGGTACTGTT 
GCATTAAGCG AGGAGACAGT TCAATTCGCC AGGGCTGGCT GTCGTCCCGC TACGTTACAC 
GCAGCGCTAC AAAAGTCTTT GAATAAACAA TTCGTTTCTT GCACCGTCAA AGCTAATAAA 
GATTTAGGCG ATGCTTCCGC TGAAAGATCC ACCATCTTTA TTGATCTGGA ACCTAAGGAG 
GAAATAAAGT ATAATCCAGG AGATCATGTC GGAATAATAG CTTGTAACCG TAAAGAGCTC 
GTTGAGAGTC TGCTCTCCCG CATTAAAGAC GTTGATGATT ACGACGAGCC GTTGCAGTTG 
CAGCTTCTGA AAGAAACTCA TACGTCAAGC GGTTTAGTCA AATCTTGGGA GCCCCACGAG 
AAACTTCCGA TAATGAGCGT GCGTGAATTG TTCACACGAT TCCTTGATAT AACAACTCCA 
CCGACCACGA TACTTCTTCA GTACTTGGCC ACAACGTGCG AAGATGAAGA AGAAAAAAAA 
CAATTGAATG TTTTGGCTAC GGATCCCGGA GCTTACGAAG ATTGGCGTCA TTTCCACTTC 
CCCACCTTGC CCGAAGTCCT AGATCAGTTT CCATCAGCAA GACCCAATGC ATCTTTACTT 
GCCGCCCTTT TATCCCCACT GCAGCCAAGA TTTTACTCAA TTTCTTCGTC ACCATTAGCC 
CACGCAAAGA GATTGCATCT TACGGTTGCC GTAGTCACAT ATCGAACTCA AGATGGTGAG 
GGACCGGTCC ATTATGGTGT GTGTTCCAAC TACTTAATGG AACGTAAGCC TGGCGATGAG 
GTTTACCTCT TTATCAGAAG TGCTCCTAAT TTCCATTTAC CTCAAGATTT GTCAGTTCCT 
CTTATACTAA TTGGTCCCGG TACTGGTATA GCTCCATTCC GTGGCTTTTG GCATCATCGG 
CGAGCTCTAC AAAATTCTTG CTCGCGTACA ACCACTGGAC CGGTGTGGCT GTTCTTCGGC 
TGTCGAACGA AGACTATGGA CTTATACCGA GAAGAGAAAG AGCAAGCTTT GAAAGAAGGC 
GTACTTTCTA AAGTATTTCT CGCTCTATCT AGAGAAAAGG AAGTTCCAAA AACGTACGTC 
CAAGAAGTTG CTGAAAACGT TGGTGCAGAA ATACACGATT TACTAATTAA TAAAGGTGCA 
CACTTTTACG TTTGCGGTGA TTGTAAAATG GCGGAGGATG TGCACCAGAA GCTCAAAGGT 
ATCGTGAAGA AACACGGAAA CATGACAGAC GAACAAGTAC AGAACTTTAT GTTCATGCTA 
AAGGAAGAGA ATCGTTACCA CGAAGACATA TTCGGCATCA CACTGCGCAC AGCTGAGGTC 
CACAGCGCTT CTCGCGAGTC GGCTCGTAGG AACCGCGTCG CTTCCCAACC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116808.1
CDS	1057..4350
Translation	MAIPGRGDVPRDPEEVLNDAKDFLGQYFASIRRANTPAHEARWKIVQEEVATTGTYQLTTTELVFGAKLAWRNASRCIGRIQWSKLQVFDCRQVTTTSGMFEALCNHIKYSTNKGNIRSAITLFPQRTDGKHDYRIWNSQLISYAGYRQPDGTVLGDPMHCEFTDLCLKLGWKPPRTAWDILPLVLSANGKDPDYFEIPRELVMEIHMTHPTFEWFKELELRWYALPAVSSMRFDCGGIEFTANAFNGWYMSTEIGCRNFCDTNRLNVLEKIAQNMGLDTRTPVNLWKDKALVEVNVAVLHSFQQHNATIVDHHTASESFIKHLDNENRLRSGCPADWIWIVPPMSSSITPVFHQEMALYYLKPSYEYQEPAWKTHQWQKSKPITGKRPINRKFHFKQIARAVKFTSKLFGRALSKRIKATVLYATETGKSEQYAKELGVIFGHAFNAQVHCMADYDITSIEHEALLLVVTSTFGNGDPPENGVAFGEHLCEILYADQVGEDSTGNQMLTPKSFIKANSDIQRYTAGNPKKLNRLESLKGSTTDATSIDSFGPLSNVRFAVFALGSSAYPNFCNFGKYVDKLLVDLGGERIHDLATGDEMCGQDQAFRKWASSVFNVACETFCLDDDETLQEAKRALGTVALSEETVQFARAGCRPATLHAALQKSLNKQFVSCTVKANKDLGDASAERSTIFIDLEPKEEIKYNPGDHVGIIACNRKELVESLLSRIKDVDDYDEPLQLQLLKETHTSSGLVKSWEPHEKLPIMSVRELFTRFLDITTPPTTILLQYLATTCEDEEEKKQLNVLATDPGAYEDWRHFHFPTLPEVLDQFPSARPNASLLAALLSPLQPRFYSISSSPLAHAKRLHLTVAVVTYRTQDGEGPVHYGVCSNYLMERKPGDEVYLFIRSAPNFHLPQDLSVPLILIGPGTGIAPFRGFWHHRRALQNSCSRTTTGPVWLFFGCRTKTMDLYREEKEQALKEGVLSKVFLALSREKEVPKTYVQEVAENVGAEIHDLLINKGAHFYVCGDCKMAEDVHQKLKGIVKKHGNMTDEQVQNFMFMLKEENRYHEDIFGITLRTAEVHSASRESARRNRVASQP

Target ORF information:

RefSeq Version	NM_001123336.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori nitric oxide synthase 2 (NOS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123336.1

ORF Insert Sequence:

ATGGCCATCC CGGGACGAGG CGACGTGCCC CGAGACCCGG AGGAGGTGCT TAATGACGCA 
AAGGACTTTC TAGGCCAGTA CTTCGCTTCT ATACGGAGGG CCAATACTCC AGCTCATGAA 
GCTCGCTGGA AAATCGTACA AGAGGAAGTA GCTACGACTG GCACATACCA ACTAACCACA 
ACAGAACTGG TATTTGGAGC CAAGCTTGCA TGGAGAAACG CTAGCCGTTG CATTGGCAGA 
ATCCAGTGGT CAAAATTGCA GGTATTCGAT TGTCGCCAAG TCACAACAAC TAGTGGAATG 
TTCGAAGCAC TCTGTAACCA CATCAAGTAC AGCACAAATA AAGGAAATAT AAGATCAGCT 
ATAACATTGT TCCCACAACG TACGGACGGC AAGCACGACT ACAGAATATG GAACAGCCAG 
CTGATCAGCT ACGCTGGCTA CCGTCAGCCA GACGGTACGG TGCTTGGTGA CCCCATGCAC 
TGCGAATTCA CTGATCTCTG CTTGAAACTT GGCTGGAAGC CGCCACGCAC GGCCTGGGAT 
ATTCTTCCCC TAGTTCTGTC AGCTAACGGG AAAGACCCTG ACTACTTCGA AATCCCCAGG 
GAGCTTGTTA TGGAAATCCA CATGACGCAC CCCACGTTCG AATGGTTCAA AGAACTAGAA 
TTACGTTGGT ACGCTTTGCC CGCCGTCTCC AGTATGCGTT TTGATTGCGG TGGCATCGAA 
TTCACGGCCA ACGCTTTCAA CGGTTGGTAC ATGAGCACTG AAATCGGCTG CAGGAACTTC 
TGTGACACCA ACCGGCTTAA TGTTTTGGAG AAAATAGCTC AAAACATGGG CCTAGATACC 
AGAACTCCGG TCAATCTTTG GAAGGATAAG GCCCTCGTGG AAGTGAACGT GGCTGTACTG 
CACAGCTTTC AACAGCACAA CGCGACTATC GTGGATCATC ATACAGCTTC TGAAAGCTTC 
ATAAAGCATT TGGATAACGA GAACCGGCTT CGGTCTGGAT GTCCAGCCGA TTGGATATGG 
ATCGTACCTC CCATGTCATC GTCCATAACC CCGGTGTTTC ACCAAGAAAT GGCGCTCTAC 
TACTTGAAAC CTTCATACGA ATATCAGGAG CCTGCATGGA AGACGCATCA GTGGCAGAAA 
TCTAAGCCGA TCACAGGCAA AAGACCCATA AACAGAAAGT TCCATTTCAA GCAAATTGCC 
CGAGCTGTCA AATTCACTTC GAAATTGTTC GGTCGTGCCC TTTCGAAGAG GATTAAAGCG 
ACTGTGCTGT ACGCTACGGA GACTGGGAAA TCGGAACAAT ACGCCAAAGA GCTTGGTGTT 
ATATTTGGAC ACGCCTTTAA TGCGCAGGTT CATTGTATGG CGGATTACGA TATAACTTCA 
ATTGAACACG AAGCTCTCCT TCTGGTTGTG ACATCGACGT TTGGAAATGG TGACCCACCC 
GAGAATGGTG TCGCATTTGG AGAGCATCTA TGCGAAATAT TATACGCTGA TCAAGTCGGT 
GAAGATTCTA CTGGAAATCA AATGTTGACA CCAAAATCGT TCATAAAAGC CAATAGTGAC 
ATTCAAAGGT ATACAGCAGG AAATCCGAAA AAACTCAACA GACTCGAATC ATTGAAGGGT 
AGTACAACAG ACGCCACATC CATTGACAGC TTTGGTCCAT TGAGTAATGT TAGATTTGCC 
GTGTTCGCTC TGGGTTCGAG CGCTTACCCG AATTTCTGTA ACTTCGGAAA GTATGTAGAC 
AAATTGTTGG TTGACCTCGG CGGGGAACGA ATCCACGACC TCGCAACCGG TGACGAAATG 
TGCGGCCAAG ATCAGGCCTT CCGGAAGTGG GCCTCTAGCG TCTTCAACGT TGCTTGTGAG 
ACATTCTGCT TGGATGATGA TGAGACTCTA CAAGAGGCAA AGAGAGCTCT CGGTACTGTT 
GCATTAAGCG AGGAGACAGT TCAATTCGCC AGGGCTGGCT GTCGTCCCGC TACGTTACAC 
GCAGCGCTAC AAAAGTCTTT GAATAAACAA TTCGTTTCTT GCACCGTCAA AGCTAATAAA 
GATTTAGGCG ATGCTTCCGC TGAAAGATCC ACCATCTTTA TTGATCTGGA ACCTAAGGAG 
GAAATAAAGT ATAATCCAGG AGATCATGTC GGAATAATAG CTTGTAACCG TAAAGAGCTC 
GTTGAGAGTC TGCTCTCCCG CATTAAAGAC GTTGATGATT ACGACGAGCC GTTGCAGTTG 
CAGCTTCTGA AAGAAACTCA TACGTCAAGC GGTTTAGTCA AATCTTGGGA GCCCCACGAG 
AAACTTCCGA TAATGAGCGT GCGTGAATTG TTCACACGAT TCCTTGATAT AACAACTCCA 
CCGACCACGA TACTTCTTCA GTACTTGGCC ACAACGTGCG AAGATGAAGA AGAAAAAAAA 
CAATTGAATG TTTTGGCTAC GGATCCCGGA GCTTACGAAG ATTGGCGTCA TTTCCACTTC 
CCCACCTTGC CCGAAGTCCT AGATCAGTTT CCATCAGCAA GACCCAATGC ATCTTTACTT 
GCCGCCCTTT TATCCCCACT GCAGCCAAGA TTTTACTCAA TTTCTTCGTC ACCATTAGCC 
CACGCAAAGA GATTGCATCT TACGGTTGCC GTAGTCACAT ATCGAACTCA AGATGGTGAG 
GGACCGGTCC ATTATGGTGT GTGTTCCAAC TACTTAATGG AACGTAAGCC TGGCGATGAG 
GTTTACCTCT TTATCAGAAG TGCTCCTAAT TTCCATTTAC CTCAAGATTT GTCAGTTCCT 
CTTATACTAA TTGGTCCCGG TACTGGTATA GCTCCATTCC GTGGCTTTTG GCATCATCGG 
CGAGCTCTAC AAAATTCTTG CTCGCGTACA ACCACTGGAC CGGTGTGGCT GTTCTTCGGC 
TGTCGAACGA AGACTATGGA CTTATACCGA GAAGAGAAAG AGCAAGCTTT GAAAGAAGGC 
GTACTTTCTA AAGTATTTCT CGCTCTATCT AGAGAAAAGG AAGTTCCAAA AACGTACGTC 
CAAGAAGTTG CTGAAAACGT TGGTGCAGAA ATACACGATT TACTAATTAA TAAAGGTGCA 
CACTTTTACG TTTGCGGTGA TTGTAAAATG GCGGAGGATG TGCACCAGAA GCTCAAAGGT 
ATCGTGAAGA AACACGGAAA CATGACAGAC GAACAAGTAC AGAACTTTAT GTTCATGCTA 
AAGGAAGAGA ATCGTTACCA CGAAGACATA TTCGGCATCA CACTGCGCAC AGCTGAGGTC 
CACAGCGCTT CTCGCGAGTC GGCTCGTAGG AACCGCGTCG CTTCCCAACC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( XM_012689821.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_012545275.1 Bombyx mori nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBg14309
Clone ID Related Accession (Same CDS sequence)	XM_012689821.1 , XM_012689821.2
Accession Version	XM_012689821.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433347200000
Organism	Bombyx mori(Domestic silkworm)
Product	nitric oxide synthase-like protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004581679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCCG GTGTCATTCA AAACTCTGAA ACAATATTGA CCGACTCCCT CGAAGTTATT 
ACCGCGACGC ATAATGGGGG TCTTATTAAC GAACTGATGG TTGAATCTAA TGTAAACAAA 
ACGTGCCCAG TGGATCTACA GTTCATTAAT GGAGCCCATG GGCAACATCA AGCTGTTAAG 
GCTGCTGAAG CGGATAAAGT TGAAGTCAAG AAGCCGGCTC GTAAAGTTAC AAGACCCAAA 
GTGCTGAAGA ACCACGAGTC CAGAGAATTA TTGTACGACA GACTGTACGA CACAACTTGT 
CCCAGAGTGC CGACGCTATG CAACGAGCGC TCGTGTCTTG GGAGTTTGAT GGCCATCCCG 
GGACGAGGCG ACGTGCCCCG AGACCCGGAG GAGGTGCTTA ATGACGCAAA GGACTTTCTA 
GGCCAGTACT TCGCTTCTAT ACGGAGGGCC AATACTCCAG CTCATGAAGC TCGCTGGAAA 
ATCGTACAAG AGGAAGTAGC TACGACTGGC ACATACCAAC TAACCACAAC AGAACTGGTA 
TTTGGAGCCA AGCTTGCATG GAGAAACGCT AGCCGTTGCA TTGGCAGAAT CCAGTGGTCA 
AAATTGCAGG TATTCGATTG TCGCCAAGTC ACAACAACTA GTGGAATGTT CGAAGCACTC 
TGTAACCACA TCAAGTACAG CACAAATAAA GGAAATATAA GATCAGCTAT AACATTGTTC 
CCACAACGTA CGGACGGCAA GCACGACTAC AGAATATGGA ACAGCCAGCT GATCAGCTAC 
GCTGGCTACC GTCAGCCAGA CGGTACGGTG CTTGGTGACC CCATGCACTG CGAATTCACT 
GATCTCTGCT TGAAACTTGG CTGGAAGCCG CCACGCACGG CCTGGGATAT TCTTCCCCTA 
GTTCTGTCAG CTAACGGGAA AGACCCTGAC TACTTCGAAA TCCCCAGGGA GCTTGTTATG 
GAAATCCACA TGACGCACCC CACGTTCGAA TGGTTCAAAG AACTAGAATT ACGTTGGTAC 
GCTTTGCCCG CCGTCTCCAG TATGCGTTTT GATTGCGGTG GCATCGAATT CACGGCCAAC 
GCTTTCAACG GTTGGTACAT GAGCACTGAA ATCGGCTGCA GGAACTTCTG TGACACCAAC 
CGGCTTAATG TTTTGGAGAA AATAGCTCAA AACATGGGCC TAGATACCAG AACTCCGGTC 
AATCTTTGGA AGGATAAGGC CCTCGTGGAA GTGAACGTGG CTGTACTGCA CAGCTTTCAA 
CAGCACAACG CGACTATCGT GGATCATCAT ACAGCTTCTG AAAGCTTCAT AAAGCATTTG 
GATAACGAGA ACCGGCTTCG GTCTGGATGT CCAGCCGATT GGATATGGAT CGTACCTCCC 
ATGTCATCGT CCATAACCCC GGTGTTTCAC CAAGAAATGG CGCTCTACTA CTTGAAACCT 
TCATACGAAT ATCAGGAGCC TGCATGGAAG ACGCATCAGT GGCAGAAATC TAAGCCGATC 
ACAGGCAAAA GACCCATAAA CAGAAAGTTC CATTTCAAGC AAATTGCCCG AGCTGTCAAA 
TTCACTTCGA AATTGTTCGG TCGTGCCCTT TCGAAGAGGA TTAAAGCGAC TGTGCTGTAC 
GCTACGGAGA CTGGGAAATC GGAACAATAC GCCAAAGAGC TTGGTGTTAT ATTTGGACAC 
GCCTTTAATG CGCAGGTTCA TTGTATGGCG GATTACGATA TAACTTCAAT TGAACACGAA 
GCTCTCCTTC TGGTTGTGAC ATCGACGTTT GGAAATGGTG ACCCACCCGA GAATGGTGTC 
GCATTTGGAG AGCATCTATG CGAAATATTA TACGCTGATC AAGTCGGTGA AGATTCTACT 
GGAAATCAAA TGTTGACACC AAAATCGTTC ATAAAAGCCA ATAGTGACAT TCAAAGGTAT 
ACAGCAGGAA ATCCGAAAAA ACTCAACAGA CTCGAATCAT TGAAGGGTAG TACAACAGAC 
GCCACATCCA TTGACAGCTT TGGTCCATTG AGTAATGTTA GATTTGCCGT GTTCGCTCTG 
GGTTCGAGCG CTTACCCGAA TTTCTGTAAC TTCGGAAAGT ATGTAGACAA ATTGTTGGTT 
GACCTCGGCG GGGAACGAAT CCACGACCTC GCAACCGGTG ACGAAATGTG CGGCCAAGAT 
CAGGCCTTCC GGAAGTGGGC CTCTAGCGTC TTCAACGTTG CTTGTGAGAC ATTCTGCTTG 
GATGATGATG AGACTCTACA AGAGGCAAAG AGAGCTCTCG GTACTGTTGC ATTAAGCGAG 
GAGACAGTTC AATTCGCCAG GGCTGGCTGT CGTCCCGCTA CGTTACACGC AGCGCTACAA 
AAGTCTTTGA ATAAACAATT CGTTTCTTGC ACCGTCAAAG CTAATAAAGA TTTAGGCGAT 
GCTTCCGCTG AAAGATCCAC CATCTTTATT GATCTGGAAC CTAAGGAGGA AATAAAGTAT 
AATCCAGGAG ATCATGTCGG AATAATAGCT TGTAACCGTA AAGAGCTCGT TGAGAGTCTG 
CTCTCCCGCA TTAAAGACGT TGATGATTAC GACGAGCCGT TGCAGTTGCA GCTTCTGAAA 
GAAACTCATA CGTCAAGCGG TTTAGTCAAA TCTTGGGAGC CCCACGAGAA ACTTCCGATA 
ATGAGCGTGC GTGAATTGTT CACACGATTC CTTGATATAA CAACTCCACC GACCACGATA 
CTTCTTCAGT ACTTGGCCAC AACGTGCGAA GATGAAGAAG AAAAAAAACA ATTGAATGTT 
TTGGCTACGG ATCCCGGAGC TTACGAAGAT TGGCGTCATT TCCACTTCCC CACCTTGCCC 
GAAGTCCTAG ATCAGTTTCC ATCAGCAAGA CCCAATGCAT CTTTACTTGC CGCCCTTTTA 
TCCCCACTGC AGCCAAGATT TTACTCAATT TCTTCGTCAC CATTAGCCCA CGCAAAGAGA 
TTGCATCTTA CGGTTGCCGT AGTCACATAT CGAACTCAAG ATGGTGAGGG ACCGGTCCAT 
TATGGTGTGT GTTCCAACTA CTTAATGGAA CGTAAGCCTG GCGATGAGGT TTACCTCTTT 
ATCAGAAGTG CTCCTAATTT CCATTTACCT CAAGATTTGT CAGTTCCTCT TATACTAATT 
GGTCCCGGTA CTGGTATAGC TCCATTCCGT GGCTTTTGGC ATCATCGGCG AGCTCTACAA 
AATTCTTGCT CGCGTACAAC CACTGGACCG GTGTGGCTGT TCTTCGGCTG TCGAACGAAG 
ACTATGGACT TATACCGAGA AGAGAAAGAG CAAGCTTTGA AAGAAGGCGT ACTTTCTAAA 
GTATTTCTCG CTCTATCTAG AGAAAAGGAA GTTCCAAAAA CGTACGTCCA AGAAGTTGCT 
GAAAACGTTG GTGCAGAAAT ACACGATTTA CTAATTAATA AAGGTGCACA CTTTTACGTT 
TGCGGTGATT GTAAAATGGC GGAGGATGTG CACCAGAAGC TCAAAGGTAT CGTGAAGAAA 
CACGGAAACA TGACAGACGA ACAAGTACAG AACTTTATGT TCATGCTAAA GGAAGAGAAT 
CGTTACCACG AAGACATATT CGGCATCACA CTGCGCACAG CTGAGGTCCA CAGCGCTTCT 
CGCGAGTCGG CTCGTAGGAA CCGCGTCGCT TCCCAACCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012545275.1
CDS	95..3736
Translation	MAPGVIQNSETILTDSLEVITATHNGGLINELMVESNVNKTCPVDLQFINGAHGQHQAVKAAEADKVEVKKPARKVTRPKVLKNHESRELLYDRLYDTTCPRVPTLCNERSCLGSLMAIPGRGDVPRDPEEVLNDAKDFLGQYFASIRRANTPAHEARWKIVQEEVATTGTYQLTTTELVFGAKLAWRNASRCIGRIQWSKLQVFDCRQVTTTSGMFEALCNHIKYSTNKGNIRSAITLFPQRTDGKHDYRIWNSQLISYAGYRQPDGTVLGDPMHCEFTDLCLKLGWKPPRTAWDILPLVLSANGKDPDYFEIPRELVMEIHMTHPTFEWFKELELRWYALPAVSSMRFDCGGIEFTANAFNGWYMSTEIGCRNFCDTNRLNVLEKIAQNMGLDTRTPVNLWKDKALVEVNVAVLHSFQQHNATIVDHHTASESFIKHLDNENRLRSGCPADWIWIVPPMSSSITPVFHQEMALYYLKPSYEYQEPAWKTHQWQKSKPITGKRPINRKFHFKQIARAVKFTSKLFGRALSKRIKATVLYATETGKSEQYAKELGVIFGHAFNAQVHCMADYDITSIEHEALLLVVTSTFGNGDPPENGVAFGEHLCEILYADQVGEDSTGNQMLTPKSFIKANSDIQRYTAGNPKKLNRLESLKGSTTDATSIDSFGPLSNVRFAVFALGSSAYPNFCNFGKYVDKLLVDLGGERIHDLATGDEMCGQDQAFRKWASSVFNVACETFCLDDDETLQEAKRALGTVALSEETVQFARAGCRPATLHAALQKSLNKQFVSCTVKANKDLGDASAERSTIFIDLEPKEEIKYNPGDHVGIIACNRKELVESLLSRIKDVDDYDEPLQLQLLKETHTSSGLVKSWEPHEKLPIMSVRELFTRFLDITTPPTTILLQYLATTCEDEEEKKQLNVLATDPGAYEDWRHFHFPTLPEVLDQFPSARPNASLLAALLSPLQPRFYSISSSPLAHAKRLHLTVAVVTYRTQDGEGPVHYGVCSNYLMERKPGDEVYLFIRSAPNFHLPQDLSVPLILIGPGTGIAPFRGFWHHRRALQNSCSRTTTGPVWLFFGCRTKTMDLYREEKEQALKEGVLSKVFLALSREKEVPKTYVQEVAENVGAEIHDLLINKGAHFYVCGDCKMAEDVHQKLKGIVKKHGNMTDEQVQNFMFMLKEENRYHEDIFGITLRTAEVHSASRESARRNRVASQP

Target ORF information:

RefSeq Version	XM_012689821.1
Organism	Bombyx mori(Domestic silkworm)
Definition	Bombyx mori nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012689821.1

ORF Insert Sequence:

ATGGCTCCCG GTGTCATTCA AAACTCTGAA ACAATATTGA CCGACTCCCT CGAAGTTATT 
ACCGCGACGC ATAATGGGGG TCTTATTAAC GAACTGATGG TTGAATCTAA TGTAAACAAA 
ACGTGCCCAG TGGATCTACA GTTCATTAAT GGAGCCCATG GGCAACATCA AGCTGTTAAG 
GCTGCTGAAG CGGATAAAGT TGAAGTCAAG AAGCCGGCTC GTAAAGTTAC AAGACCCAAA 
GTGCTGAAGA ACCACGAGTC CAGAGAATTA TTGTACGACA GACTGTACGA CACAACTTGT 
CCCAGAGTGC CGACGCTATG CAACGAGCGC TCGTGTCTTG GGAGTTTGAT GGCCATCCCG 
GGACGAGGCG ACGTGCCCCG AGACCCGGAG GAGGTGCTTA ATGACGCAAA GGACTTTCTA 
GGCCAGTACT TCGCTTCTAT ACGGAGGGCC AATACTCCAG CTCATGAAGC TCGCTGGAAA 
ATCGTACAAG AGGAAGTAGC TACGACTGGC ACATACCAAC TAACCACAAC AGAACTGGTA 
TTTGGAGCCA AGCTTGCATG GAGAAACGCT AGCCGTTGCA TTGGCAGAAT CCAGTGGTCA 
AAATTGCAGG TATTCGATTG TCGCCAAGTC ACAACAACTA GTGGAATGTT CGAAGCACTC 
TGTAACCACA TCAAGTACAG CACAAATAAA GGAAATATAA GATCAGCTAT AACATTGTTC 
CCACAACGTA CGGACGGCAA GCACGACTAC AGAATATGGA ACAGCCAGCT GATCAGCTAC 
GCTGGCTACC GTCAGCCAGA CGGTACGGTG CTTGGTGACC CCATGCACTG CGAATTCACT 
GATCTCTGCT TGAAACTTGG CTGGAAGCCG CCACGCACGG CCTGGGATAT TCTTCCCCTA 
GTTCTGTCAG CTAACGGGAA AGACCCTGAC TACTTCGAAA TCCCCAGGGA GCTTGTTATG 
GAAATCCACA TGACGCACCC CACGTTCGAA TGGTTCAAAG AACTAGAATT ACGTTGGTAC 
GCTTTGCCCG CCGTCTCCAG TATGCGTTTT GATTGCGGTG GCATCGAATT CACGGCCAAC 
GCTTTCAACG GTTGGTACAT GAGCACTGAA ATCGGCTGCA GGAACTTCTG TGACACCAAC 
CGGCTTAATG TTTTGGAGAA AATAGCTCAA AACATGGGCC TAGATACCAG AACTCCGGTC 
AATCTTTGGA AGGATAAGGC CCTCGTGGAA GTGAACGTGG CTGTACTGCA CAGCTTTCAA 
CAGCACAACG CGACTATCGT GGATCATCAT ACAGCTTCTG AAAGCTTCAT AAAGCATTTG 
GATAACGAGA ACCGGCTTCG GTCTGGATGT CCAGCCGATT GGATATGGAT CGTACCTCCC 
ATGTCATCGT CCATAACCCC GGTGTTTCAC CAAGAAATGG CGCTCTACTA CTTGAAACCT 
TCATACGAAT ATCAGGAGCC TGCATGGAAG ACGCATCAGT GGCAGAAATC TAAGCCGATC 
ACAGGCAAAA GACCCATAAA CAGAAAGTTC CATTTCAAGC AAATTGCCCG AGCTGTCAAA 
TTCACTTCGA AATTGTTCGG TCGTGCCCTT TCGAAGAGGA TTAAAGCGAC TGTGCTGTAC 
GCTACGGAGA CTGGGAAATC GGAACAATAC GCCAAAGAGC TTGGTGTTAT ATTTGGACAC 
GCCTTTAATG CGCAGGTTCA TTGTATGGCG GATTACGATA TAACTTCAAT TGAACACGAA 
GCTCTCCTTC TGGTTGTGAC ATCGACGTTT GGAAATGGTG ACCCACCCGA GAATGGTGTC 
GCATTTGGAG AGCATCTATG CGAAATATTA TACGCTGATC AAGTCGGTGA AGATTCTACT 
GGAAATCAAA TGTTGACACC AAAATCGTTC ATAAAAGCCA ATAGTGACAT TCAAAGGTAT 
ACAGCAGGAA ATCCGAAAAA ACTCAACAGA CTCGAATCAT TGAAGGGTAG TACAACAGAC 
GCCACATCCA TTGACAGCTT TGGTCCATTG AGTAATGTTA GATTTGCCGT GTTCGCTCTG 
GGTTCGAGCG CTTACCCGAA TTTCTGTAAC TTCGGAAAGT ATGTAGACAA ATTGTTGGTT 
GACCTCGGCG GGGAACGAAT CCACGACCTC GCAACCGGTG ACGAAATGTG CGGCCAAGAT 
CAGGCCTTCC GGAAGTGGGC CTCTAGCGTC TTCAACGTTG CTTGTGAGAC ATTCTGCTTG 
GATGATGATG AGACTCTACA AGAGGCAAAG AGAGCTCTCG GTACTGTTGC ATTAAGCGAG 
GAGACAGTTC AATTCGCCAG GGCTGGCTGT CGTCCCGCTA CGTTACACGC AGCGCTACAA 
AAGTCTTTGA ATAAACAATT CGTTTCTTGC ACCGTCAAAG CTAATAAAGA TTTAGGCGAT 
GCTTCCGCTG AAAGATCCAC CATCTTTATT GATCTGGAAC CTAAGGAGGA AATAAAGTAT 
AATCCAGGAG ATCATGTCGG AATAATAGCT TGTAACCGTA AAGAGCTCGT TGAGAGTCTG 
CTCTCCCGCA TTAAAGACGT TGATGATTAC GACGAGCCGT TGCAGTTGCA GCTTCTGAAA 
GAAACTCATA CGTCAAGCGG TTTAGTCAAA TCTTGGGAGC CCCACGAGAA ACTTCCGATA 
ATGAGCGTGC GTGAATTGTT CACACGATTC CTTGATATAA CAACTCCACC GACCACGATA 
CTTCTTCAGT ACTTGGCCAC AACGTGCGAA GATGAAGAAG AAAAAAAACA ATTGAATGTT 
TTGGCTACGG ATCCCGGAGC TTACGAAGAT TGGCGTCATT TCCACTTCCC CACCTTGCCC 
GAAGTCCTAG ATCAGTTTCC ATCAGCAAGA CCCAATGCAT CTTTACTTGC CGCCCTTTTA 
TCCCCACTGC AGCCAAGATT TTACTCAATT TCTTCGTCAC CATTAGCCCA CGCAAAGAGA 
TTGCATCTTA CGGTTGCCGT AGTCACATAT CGAACTCAAG ATGGTGAGGG ACCGGTCCAT 
TATGGTGTGT GTTCCAACTA CTTAATGGAA CGTAAGCCTG GCGATGAGGT TTACCTCTTT 
ATCAGAAGTG CTCCTAATTT CCATTTACCT CAAGATTTGT CAGTTCCTCT TATACTAATT 
GGTCCCGGTA CTGGTATAGC TCCATTCCGT GGCTTTTGGC ATCATCGGCG AGCTCTACAA 
AATTCTTGCT CGCGTACAAC CACTGGACCG GTGTGGCTGT TCTTCGGCTG TCGAACGAAG 
ACTATGGACT TATACCGAGA AGAGAAAGAG CAAGCTTTGA AAGAAGGCGT ACTTTCTAAA 
GTATTTCTCG CTCTATCTAG AGAAAAGGAA GTTCCAAAAA CGTACGTCCA AGAAGTTGCT 
GAAAACGTTG GTGCAGAAAT ACACGATTTA CTAATTAATA AAGGTGCACA CTTTTACGTT 
TGCGGTGATT GTAAAATGGC GGAGGATGTG CACCAGAAGC TCAAAGGTAT CGTGAAGAAA 
CACGGAAACA TGACAGACGA ACAAGTACAG AACTTTATGT TCATGCTAAA GGAAGAGAAT 
CGTTACCACG AAGACATATT CGGCATCACA CTGCGCACAG CTGAGGTCCA CAGCGCTTCT 
CGCGAGTCGG CTCGTAGGAA CCGCGTCGCT TCCCAACCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( XM_012689821.2 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_012545275.1 Bombyx mori nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.

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CloneID	OBg14309
Clone ID Related Accession (Same CDS sequence)	XM_012689821.1 , XM_012689821.2
Accession Version	XM_012689821.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1496505600000
Organism	Bombyx mori(domestic silkworm)
Product	nitric oxide synthase-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004581679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 5, 2017 this sequence version replaced XM_012689821.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCCG GTGTCATTCA AAACTCTGAA ACAATATTGA CCGACTCCCT CGAAGTTATT 
ACCGCGACGC ATAATGGGGG TCTTATTAAC GAACTGATGG TTGAATCTAA TGTAAACAAA 
ACGTGCCCAG TGGATCTACA GTTCATTAAT GGAGCCCATG GGCAACATCA AGCTGTTAAG 
GCTGCTGAAG CGGATAAAGT TGAAGTCAAG AAGCCGGCTC GTAAAGTTAC AAGACCCAAA 
GTGCTGAAGA ACCACGAGTC CAGAGAATTA TTGTACGACA GACTGTACGA CACAACTTGT 
CCCAGAGTGC CGACGCTATG CAACGAGCGC TCGTGTCTTG GGAGTTTGAT GGCCATCCCG 
GGACGAGGCG ACGTGCCCCG AGACCCGGAG GAGGTGCTTA ATGACGCAAA GGACTTTCTA 
GGCCAGTACT TCGCTTCTAT ACGGAGGGCC AATACTCCAG CTCATGAAGC TCGCTGGAAA 
ATCGTACAAG AGGAAGTAGC TACGACTGGC ACATACCAAC TAACCACAAC AGAACTGGTA 
TTTGGAGCCA AGCTTGCATG GAGAAACGCT AGCCGTTGCA TTGGCAGAAT CCAGTGGTCA 
AAATTGCAGG TATTCGATTG TCGCCAAGTC ACAACAACTA GTGGAATGTT CGAAGCACTC 
TGTAACCACA TCAAGTACAG CACAAATAAA GGAAATATAA GATCAGCTAT AACATTGTTC 
CCACAACGTA CGGACGGCAA GCACGACTAC AGAATATGGA ACAGCCAGCT GATCAGCTAC 
GCTGGCTACC GTCAGCCAGA CGGTACGGTG CTTGGTGACC CCATGCACTG CGAATTCACT 
GATCTCTGCT TGAAACTTGG CTGGAAGCCG CCACGCACGG CCTGGGATAT TCTTCCCCTA 
GTTCTGTCAG CTAACGGGAA AGACCCTGAC TACTTCGAAA TCCCCAGGGA GCTTGTTATG 
GAAATCCACA TGACGCACCC CACGTTCGAA TGGTTCAAAG AACTAGAATT ACGTTGGTAC 
GCTTTGCCCG CCGTCTCCAG TATGCGTTTT GATTGCGGTG GCATCGAATT CACGGCCAAC 
GCTTTCAACG GTTGGTACAT GAGCACTGAA ATCGGCTGCA GGAACTTCTG TGACACCAAC 
CGGCTTAATG TTTTGGAGAA AATAGCTCAA AACATGGGCC TAGATACCAG AACTCCGGTC 
AATCTTTGGA AGGATAAGGC CCTCGTGGAA GTGAACGTGG CTGTACTGCA CAGCTTTCAA 
CAGCACAACG CGACTATCGT GGATCATCAT ACAGCTTCTG AAAGCTTCAT AAAGCATTTG 
GATAACGAGA ACCGGCTTCG GTCTGGATGT CCAGCCGATT GGATATGGAT CGTACCTCCC 
ATGTCATCGT CCATAACCCC GGTGTTTCAC CAAGAAATGG CGCTCTACTA CTTGAAACCT 
TCATACGAAT ATCAGGAGCC TGCATGGAAG ACGCATCAGT GGCAGAAATC TAAGCCGATC 
ACAGGCAAAA GACCCATAAA CAGAAAGTTC CATTTCAAGC AAATTGCCCG AGCTGTCAAA 
TTCACTTCGA AATTGTTCGG TCGTGCCCTT TCGAAGAGGA TTAAAGCGAC TGTGCTGTAC 
GCTACGGAGA CTGGGAAATC GGAACAATAC GCCAAAGAGC TTGGTGTTAT ATTTGGACAC 
GCCTTTAATG CGCAGGTTCA TTGTATGGCG GATTACGATA TAACTTCAAT TGAACACGAA 
GCTCTCCTTC TGGTTGTGAC ATCGACGTTT GGAAATGGTG ACCCACCCGA GAATGGTGTC 
GCATTTGGAG AGCATCTATG CGAAATATTA TACGCTGATC AAGTCGGTGA AGATTCTACT 
GGAAATCAAA TGTTGACACC AAAATCGTTC ATAAAAGCCA ATAGTGACAT TCAAAGGTAT 
ACAGCAGGAA ATCCGAAAAA ACTCAACAGA CTCGAATCAT TGAAGGGTAG TACAACAGAC 
GCCACATCCA TTGACAGCTT TGGTCCATTG AGTAATGTTA GATTTGCCGT GTTCGCTCTG 
GGTTCGAGCG CTTACCCGAA TTTCTGTAAC TTCGGAAAGT ATGTAGACAA ATTGTTGGTT 
GACCTCGGCG GGGAACGAAT CCACGACCTC GCAACCGGTG ACGAAATGTG CGGCCAAGAT 
CAGGCCTTCC GGAAGTGGGC CTCTAGCGTC TTCAACGTTG CTTGTGAGAC ATTCTGCTTG 
GATGATGATG AGACTCTACA AGAGGCAAAG AGAGCTCTCG GTACTGTTGC ATTAAGCGAG 
GAGACAGTTC AATTCGCCAG GGCTGGCTGT CGTCCCGCTA CGTTACACGC AGCGCTACAA 
AAGTCTTTGA ATAAACAATT CGTTTCTTGC ACCGTCAAAG CTAATAAAGA TTTAGGCGAT 
GCTTCCGCTG AAAGATCCAC CATCTTTATT GATCTGGAAC CTAAGGAGGA AATAAAGTAT 
AATCCAGGAG ATCATGTCGG AATAATAGCT TGTAACCGTA AAGAGCTCGT TGAGAGTCTG 
CTCTCCCGCA TTAAAGACGT TGATGATTAC GACGAGCCGT TGCAGTTGCA GCTTCTGAAA 
GAAACTCATA CGTCAAGCGG TTTAGTCAAA TCTTGGGAGC CCCACGAGAA ACTTCCGATA 
ATGAGCGTGC GTGAATTGTT CACACGATTC CTTGATATAA CAACTCCACC GACCACGATA 
CTTCTTCAGT ACTTGGCCAC AACGTGCGAA GATGAAGAAG AAAAAAAACA ATTGAATGTT 
TTGGCTACGG ATCCCGGAGC TTACGAAGAT TGGCGTCATT TCCACTTCCC CACCTTGCCC 
GAAGTCCTAG ATCAGTTTCC ATCAGCAAGA CCCAATGCAT CTTTACTTGC CGCCCTTTTA 
TCCCCACTGC AGCCAAGATT TTACTCAATT TCTTCGTCAC CATTAGCCCA CGCAAAGAGA 
TTGCATCTTA CGGTTGCCGT AGTCACATAT CGAACTCAAG ATGGTGAGGG ACCGGTCCAT 
TATGGTGTGT GTTCCAACTA CTTAATGGAA CGTAAGCCTG GCGATGAGGT TTACCTCTTT 
ATCAGAAGTG CTCCTAATTT CCATTTACCT CAAGATTTGT CAGTTCCTCT TATACTAATT 
GGTCCCGGTA CTGGTATAGC TCCATTCCGT GGCTTTTGGC ATCATCGGCG AGCTCTACAA 
AATTCTTGCT CGCGTACAAC CACTGGACCG GTGTGGCTGT TCTTCGGCTG TCGAACGAAG 
ACTATGGACT TATACCGAGA AGAGAAAGAG CAAGCTTTGA AAGAAGGCGT ACTTTCTAAA 
GTATTTCTCG CTCTATCTAG AGAAAAGGAA GTTCCAAAAA CGTACGTCCA AGAAGTTGCT 
GAAAACGTTG GTGCAGAAAT ACACGATTTA CTAATTAATA AAGGTGCACA CTTTTACGTT 
TGCGGTGATT GTAAAATGGC GGAGGATGTG CACCAGAAGC TCAAAGGTAT CGTGAAGAAA 
CACGGAAACA TGACAGACGA ACAAGTACAG AACTTTATGT TCATGCTAAA GGAAGAGAAT 
CGTTACCACG AAGACATATT CGGCATCACA CTGCGCACAG CTGAGGTCCA CAGCGCTTCT 
CGCGAGTCGG CTCGTAGGAA CCGCGTCGCT TCCCAACCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012545275.1
CDS	103..3744
Translation	MAPGVIQNSETILTDSLEVITATHNGGLINELMVESNVNKTCPVDLQFINGAHGQHQAVKAAEADKVEVKKPARKVTRPKVLKNHESRELLYDRLYDTTCPRVPTLCNERSCLGSLMAIPGRGDVPRDPEEVLNDAKDFLGQYFASIRRANTPAHEARWKIVQEEVATTGTYQLTTTELVFGAKLAWRNASRCIGRIQWSKLQVFDCRQVTTTSGMFEALCNHIKYSTNKGNIRSAITLFPQRTDGKHDYRIWNSQLISYAGYRQPDGTVLGDPMHCEFTDLCLKLGWKPPRTAWDILPLVLSANGKDPDYFEIPRELVMEIHMTHPTFEWFKELELRWYALPAVSSMRFDCGGIEFTANAFNGWYMSTEIGCRNFCDTNRLNVLEKIAQNMGLDTRTPVNLWKDKALVEVNVAVLHSFQQHNATIVDHHTASESFIKHLDNENRLRSGCPADWIWIVPPMSSSITPVFHQEMALYYLKPSYEYQEPAWKTHQWQKSKPITGKRPINRKFHFKQIARAVKFTSKLFGRALSKRIKATVLYATETGKSEQYAKELGVIFGHAFNAQVHCMADYDITSIEHEALLLVVTSTFGNGDPPENGVAFGEHLCEILYADQVGEDSTGNQMLTPKSFIKANSDIQRYTAGNPKKLNRLESLKGSTTDATSIDSFGPLSNVRFAVFALGSSAYPNFCNFGKYVDKLLVDLGGERIHDLATGDEMCGQDQAFRKWASSVFNVACETFCLDDDETLQEAKRALGTVALSEETVQFARAGCRPATLHAALQKSLNKQFVSCTVKANKDLGDASAERSTIFIDLEPKEEIKYNPGDHVGIIACNRKELVESLLSRIKDVDDYDEPLQLQLLKETHTSSGLVKSWEPHEKLPIMSVRELFTRFLDITTPPTTILLQYLATTCEDEEEKKQLNVLATDPGAYEDWRHFHFPTLPEVLDQFPSARPNASLLAALLSPLQPRFYSISSSPLAHAKRLHLTVAVVTYRTQDGEGPVHYGVCSNYLMERKPGDEVYLFIRSAPNFHLPQDLSVPLILIGPGTGIAPFRGFWHHRRALQNSCSRTTTGPVWLFFGCRTKTMDLYREEKEQALKEGVLSKVFLALSREKEVPKTYVQEVAENVGAEIHDLLINKGAHFYVCGDCKMAEDVHQKLKGIVKKHGNMTDEQVQNFMFMLKEENRYHEDIFGITLRTAEVHSASRESARRNRVASQP

Target ORF information:

RefSeq Version	XM_012689821.2
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012689821.2

ORF Insert Sequence:

ATGGCTCCCG GTGTCATTCA AAACTCTGAA ACAATATTGA CCGACTCCCT CGAAGTTATT 
ACCGCGACGC ATAATGGGGG TCTTATTAAC GAACTGATGG TTGAATCTAA TGTAAACAAA 
ACGTGCCCAG TGGATCTACA GTTCATTAAT GGAGCCCATG GGCAACATCA AGCTGTTAAG 
GCTGCTGAAG CGGATAAAGT TGAAGTCAAG AAGCCGGCTC GTAAAGTTAC AAGACCCAAA 
GTGCTGAAGA ACCACGAGTC CAGAGAATTA TTGTACGACA GACTGTACGA CACAACTTGT 
CCCAGAGTGC CGACGCTATG CAACGAGCGC TCGTGTCTTG GGAGTTTGAT GGCCATCCCG 
GGACGAGGCG ACGTGCCCCG AGACCCGGAG GAGGTGCTTA ATGACGCAAA GGACTTTCTA 
GGCCAGTACT TCGCTTCTAT ACGGAGGGCC AATACTCCAG CTCATGAAGC TCGCTGGAAA 
ATCGTACAAG AGGAAGTAGC TACGACTGGC ACATACCAAC TAACCACAAC AGAACTGGTA 
TTTGGAGCCA AGCTTGCATG GAGAAACGCT AGCCGTTGCA TTGGCAGAAT CCAGTGGTCA 
AAATTGCAGG TATTCGATTG TCGCCAAGTC ACAACAACTA GTGGAATGTT CGAAGCACTC 
TGTAACCACA TCAAGTACAG CACAAATAAA GGAAATATAA GATCAGCTAT AACATTGTTC 
CCACAACGTA CGGACGGCAA GCACGACTAC AGAATATGGA ACAGCCAGCT GATCAGCTAC 
GCTGGCTACC GTCAGCCAGA CGGTACGGTG CTTGGTGACC CCATGCACTG CGAATTCACT 
GATCTCTGCT TGAAACTTGG CTGGAAGCCG CCACGCACGG CCTGGGATAT TCTTCCCCTA 
GTTCTGTCAG CTAACGGGAA AGACCCTGAC TACTTCGAAA TCCCCAGGGA GCTTGTTATG 
GAAATCCACA TGACGCACCC CACGTTCGAA TGGTTCAAAG AACTAGAATT ACGTTGGTAC 
GCTTTGCCCG CCGTCTCCAG TATGCGTTTT GATTGCGGTG GCATCGAATT CACGGCCAAC 
GCTTTCAACG GTTGGTACAT GAGCACTGAA ATCGGCTGCA GGAACTTCTG TGACACCAAC 
CGGCTTAATG TTTTGGAGAA AATAGCTCAA AACATGGGCC TAGATACCAG AACTCCGGTC 
AATCTTTGGA AGGATAAGGC CCTCGTGGAA GTGAACGTGG CTGTACTGCA CAGCTTTCAA 
CAGCACAACG CGACTATCGT GGATCATCAT ACAGCTTCTG AAAGCTTCAT AAAGCATTTG 
GATAACGAGA ACCGGCTTCG GTCTGGATGT CCAGCCGATT GGATATGGAT CGTACCTCCC 
ATGTCATCGT CCATAACCCC GGTGTTTCAC CAAGAAATGG CGCTCTACTA CTTGAAACCT 
TCATACGAAT ATCAGGAGCC TGCATGGAAG ACGCATCAGT GGCAGAAATC TAAGCCGATC 
ACAGGCAAAA GACCCATAAA CAGAAAGTTC CATTTCAAGC AAATTGCCCG AGCTGTCAAA 
TTCACTTCGA AATTGTTCGG TCGTGCCCTT TCGAAGAGGA TTAAAGCGAC TGTGCTGTAC 
GCTACGGAGA CTGGGAAATC GGAACAATAC GCCAAAGAGC TTGGTGTTAT ATTTGGACAC 
GCCTTTAATG CGCAGGTTCA TTGTATGGCG GATTACGATA TAACTTCAAT TGAACACGAA 
GCTCTCCTTC TGGTTGTGAC ATCGACGTTT GGAAATGGTG ACCCACCCGA GAATGGTGTC 
GCATTTGGAG AGCATCTATG CGAAATATTA TACGCTGATC AAGTCGGTGA AGATTCTACT 
GGAAATCAAA TGTTGACACC AAAATCGTTC ATAAAAGCCA ATAGTGACAT TCAAAGGTAT 
ACAGCAGGAA ATCCGAAAAA ACTCAACAGA CTCGAATCAT TGAAGGGTAG TACAACAGAC 
GCCACATCCA TTGACAGCTT TGGTCCATTG AGTAATGTTA GATTTGCCGT GTTCGCTCTG 
GGTTCGAGCG CTTACCCGAA TTTCTGTAAC TTCGGAAAGT ATGTAGACAA ATTGTTGGTT 
GACCTCGGCG GGGAACGAAT CCACGACCTC GCAACCGGTG ACGAAATGTG CGGCCAAGAT 
CAGGCCTTCC GGAAGTGGGC CTCTAGCGTC TTCAACGTTG CTTGTGAGAC ATTCTGCTTG 
GATGATGATG AGACTCTACA AGAGGCAAAG AGAGCTCTCG GTACTGTTGC ATTAAGCGAG 
GAGACAGTTC AATTCGCCAG GGCTGGCTGT CGTCCCGCTA CGTTACACGC AGCGCTACAA 
AAGTCTTTGA ATAAACAATT CGTTTCTTGC ACCGTCAAAG CTAATAAAGA TTTAGGCGAT 
GCTTCCGCTG AAAGATCCAC CATCTTTATT GATCTGGAAC CTAAGGAGGA AATAAAGTAT 
AATCCAGGAG ATCATGTCGG AATAATAGCT TGTAACCGTA AAGAGCTCGT TGAGAGTCTG 
CTCTCCCGCA TTAAAGACGT TGATGATTAC GACGAGCCGT TGCAGTTGCA GCTTCTGAAA 
GAAACTCATA CGTCAAGCGG TTTAGTCAAA TCTTGGGAGC CCCACGAGAA ACTTCCGATA 
ATGAGCGTGC GTGAATTGTT CACACGATTC CTTGATATAA CAACTCCACC GACCACGATA 
CTTCTTCAGT ACTTGGCCAC AACGTGCGAA GATGAAGAAG AAAAAAAACA ATTGAATGTT 
TTGGCTACGG ATCCCGGAGC TTACGAAGAT TGGCGTCATT TCCACTTCCC CACCTTGCCC 
GAAGTCCTAG ATCAGTTTCC ATCAGCAAGA CCCAATGCAT CTTTACTTGC CGCCCTTTTA 
TCCCCACTGC AGCCAAGATT TTACTCAATT TCTTCGTCAC CATTAGCCCA CGCAAAGAGA 
TTGCATCTTA CGGTTGCCGT AGTCACATAT CGAACTCAAG ATGGTGAGGG ACCGGTCCAT 
TATGGTGTGT GTTCCAACTA CTTAATGGAA CGTAAGCCTG GCGATGAGGT TTACCTCTTT 
ATCAGAAGTG CTCCTAATTT CCATTTACCT CAAGATTTGT CAGTTCCTCT TATACTAATT 
GGTCCCGGTA CTGGTATAGC TCCATTCCGT GGCTTTTGGC ATCATCGGCG AGCTCTACAA 
AATTCTTGCT CGCGTACAAC CACTGGACCG GTGTGGCTGT TCTTCGGCTG TCGAACGAAG 
ACTATGGACT TATACCGAGA AGAGAAAGAG CAAGCTTTGA AAGAAGGCGT ACTTTCTAAA 
GTATTTCTCG CTCTATCTAG AGAAAAGGAA GTTCCAAAAA CGTACGTCCA AGAAGTTGCT 
GAAAACGTTG GTGCAGAAAT ACACGATTTA CTAATTAATA AAGGTGCACA CTTTTACGTT 
TGCGGTGATT GTAAAATGGC GGAGGATGTG CACCAGAAGC TCAAAGGTAT CGTGAAGAAA 
CACGGAAACA TGACAGACGA ACAAGTACAG AACTTTATGT TCATGCTAAA GGAAGAGAAT 
CGTTACCACG AAGACATATT CGGCATCACA CTGCGCACAG CTGAGGTCCA CAGCGCTTCT 
CGCGAGTCGG CTCGTAGGAA CCGCGTCGCT TCCCAACCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( XM_021347365.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_021203040.1 Bombyx mori nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBg22348
Clone ID Related Accession (Same CDS sequence)	XM_021347365.1 , XM_021347397.1
Accession Version	XM_021347365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1496505600000
Organism	Bombyx mori(domestic silkworm)
Product	nitric oxide synthase-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004581679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCC AACATCTGAC GAGCATGTTC GAACAGCTCG GGAACTTTAC GAAGAACAAC 
TTGACGCACA ACAGCAAGCT GGTTAAAGAG AGAGAGAATA AGTCGAGTGC TAACCACGTG 
GGCGCCGGTC CAGGCGGTGG CGGGAACCAA AACCCGTCTC CTTCGCACAA GCACGTGCTG 
GCACAGCCTT CGCTACCGCT GAAGAAGCCC TGCAAGCTAA GGAACGTCGC ATCGAAAGCA 
GAGTCTTACG ACACTCTGTA TGCGAACAGC TCACTGCCGA CGCTATGCAA CGAGCGCTCG 
TGTCTTGGGA GTTTGATGGC CATCCCGGGA CGAGGCGACG TGCCCCGAGA CCCGGAGGAG 
GTGCTTAATG ACGCAAAGGA CTTTCTAGGC CAGTACTTCG CTTCTATACG GAGGGCCAAT 
ACTCCAGCTC ATGAAGCTCG CTGGAAAATC GTACAAGAGG AAGTAGCTAC GACTGGCACA 
TACCAACTAA CCACAACAGA ACTGGTATTT GGAGCCAAGC TTGCATGGAG AAACGCTAGC 
CGTTGCATTG GCAGAATCCA GTGGTCAAAA TTGCAGGTAT TCGATTGTCG CCAAGTCACA 
ACAACTAGTG GAATGTTCGA AGCACTCTGT AACCACATCA AGTACAGCAC AAATAAAGGA 
AATATAAGAT CAGCTATAAC ATTGTTCCCA CAACGTACGG ACGGCAAGCA CGACTACAGA 
ATATGGAACA GCCAGCTGAT CAGCTACGCT GGCTACCGTC AGCCAGACGG TACGGTGCTT 
GGTGACCCCA TGCACTGCGA ATTCACTGAT CTCTGCTTGA AACTTGGCTG GAAGCCGCCA 
CGCACGGCCT GGGATATTCT TCCCCTAGTT CTGTCAGCTA ACGGGAAAGA CCCTGACTAC 
TTCGAAATCC CCAGGGAGCT TGTTATGGAA ATCCACATGA CGCACCCCAC GTTCGAATGG 
TTCAAAGAAC TAGAATTACG TTGGTACGCT TTGCCCGCCG TCTCCAGTAT GCGTTTTGAT 
TGCGGTGGCA TCGAATTCAC GGCCAACGCT TTCAACGGTT GGTACATGAG CACTGAAATC 
GGCTGCAGGA ACTTCTGTGA CACCAACCGG CTTAATGTTT TGGAGAAAAT AGCTCAAAAC 
ATGGGCCTAG ATACCAGAAC TCCGGTCAAT CTTTGGAAGG ATAAGGCCCT CGTGGAAGTG 
AACGTGGCTG TACTGCACAG CTTTCAACAG CACAACGCGA CTATCGTGGA TCATCATACA 
GCTTCTGAAA GCTTCATAAA GCATTTGGAT AACGAGAACC GGCTTCGGTC TGGATGTCCA 
GCCGATTGGA TATGGATCGT ACCTCCCATG TCATCGTCCA TAACCCCGGT GTTTCACCAA 
GAAATGGCGC TCTACTACTT GAAACCTTCA TACGAATATC AGGAGCCTGC ATGGAAGACG 
CATCAGTGGC AGAAATCTAA GCCGATCACA GGCAAAAGAC CCATAAACAG AAAGTTCCAT 
TTCAAGCAAA TTGCCCGAGC TGTCAAATTC ACTTCGAAAT TGTTCGGTCG TGCCCTTTCG 
AAGAGGATTA AAGCGACTGT GCTGTACGCT ACGGAGACTG GGAAATCGGA ACAATACGCC 
AAAGAGCTTG GTGTTATATT TGGACACGCC TTTAATGCGC AGGTTCATTG TATGGCGGAT 
TACGATATAA CTTCAATTGA ACACGAAGCT CTCCTTCTGG TTGTGACATC GACGTTTGGA 
AATGGTGACC CACCCGAGAA TGGTGTCGCA TTTGGAGAGC ATCTATGCGA AATATTATAC 
GCTGATCAAG TCGGTGAAGA TTCTACTGGA AATCAAATGT TGACACCAAA ATCGTTCATA 
AAAGCCAATA GTGACATTCA AAGGTATACA GCAGGAAATC CGAAAAAACT CAACAGACTC 
GAATCATTGA AGGGTAGTAC AACAGACGCC ACATCCATTG ACAGCTTTGG TCCATTGAGT 
AATGTTAGAT TTGCCGTGTT CGCTCTGGGT TCGAGCGCTT ACCCGAATTT CTGTAACTTC 
GGAAAGTATG TAGACAAATT GTTGGTTGAC CTCGGCGGGG AACGAATCCA CGACCTCGCA 
ACCGGTGACG AAATGTGCGG CCAAGATCAG GCCTTCCGGA AGTGGGCCTC TAGCGTCTTC 
AACGTTGCTT GTGAGACATT CTGCTTGGAT GATGATGAGA CTCTACAAGA GGCAAAGAGA 
GCTCTCGGTA CTGTTGCATT AAGCGAGGAG ACAGTTCAAT TCGCCAGGGC TGGCTGTCGT 
CCCGCTACGT TACACGCAGC GCTACAAAAG TCTTTGAATA AACAATTCGT TTCTTGCACC 
GTCAAAGCTA ATAAAGATTT AGGCGATGCT TCCGCTGAAA GATCCACCAT CTTTATTGAT 
CTGGAACCTA AGGAGGAAAT AAAGTATAAT CCAGGAGATC ATGTCGGAAT AATAGCTTGT 
AACCGTAAAG AGCTCGTTGA GAGTCTGCTC TCCCGCATTA AAGACGTTGA TGATTACGAC 
GAGCCGTTGC AGTTGCAGCT TCTGAAAGAA ACTCATACGT CAAGCGGTTT AGTCAAATCT 
TGGGAGCCCC ACGAGAAACT TCCGATAATG AGCGTGCGTG AATTGTTCAC ACGATTCCTT 
GATATAACAA CTCCACCGAC CACGATACTT CTTCAGTACT TGGCCACAAC GTGCGAAGAT 
GAAGAAGAAA AAAAACAATT GAATGTTTTG GCTACGGATC CCGGAGCTTA CGAAGATTGG 
CGTCATTTCC ACTTCCCCAC CTTGCCCGAA GTCCTAGATC AGTTTCCATC AGCAAGACCC 
AATGCATCTT TACTTGCCGC CCTTTTATCC CCACTGCAGC CAAGATTTTA CTCAATTTCT 
TCGTCACCAT TAGCCCACGC AAAGAGATTG CATCTTACGG TTGCCGTAGT CACATATCGA 
ACTCAAGATG GTGAGGGACC GGTCCATTAT GGTGTGTGTT CCAACTACTT AATGGAACGT 
AAGCCTGGCG ATGAGGTTTA CCTCTTTATC AGAAGTGCTC CTAATTTCCA TTTACCTCAA 
GATTTGTCAG TTCCTCTTAT ACTAATTGGT CCCGGTACTG GTATAGCTCC ATTCCGTGGC 
TTTTGGCATC ATCGGCGAGC TCTACAAAAT TCTTGCTCGC GTACAACCAC TGGACCGGTG 
TGGCTGTTCT TCGGCTGTCG AACGAAGACT ATGGACTTAT ACCGAGAAGA GAAAGAGCAA 
GCTTTGAAAG AAGGCGTACT TTCTAAAGTA TTTCTCGCTC TATCTAGAGA AAAGGAAGTT 
CCAAAAACGT ACGTCCAAGA AGTTGCTGAA AACGTTGGTG CAGAAATACA CGATTTACTA 
ATTAATAAAG GTGCACACTT TTACGTTTGC GGTGATTGTA AAATGGCGGA GGATGTGCAC 
CAGAAGCTCA AAGGTATCGT GAAGAAACAC GGAAACATGA CAGACGAACA AGTACAGAAC 
TTTATGTTCA TGCTAAAGGA AGAGAATCGT TACCACGAAG ACATATTCGG CATCACACTG 
CGCACAGCTG AGGTCCACAG CGCTTCTCGC GAGTCGGCTC GTAGGAACCG CGTCGCTTCC 
CAACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021203040.1
CDS	143..3751
Translation	MMSQHLTSMFEQLGNFTKNNLTHNSKLVKERENKSSANHVGAGPGGGGNQNPSPSHKHVLAQPSLPLKKPCKLRNVASKAESYDTLYANSSLPTLCNERSCLGSLMAIPGRGDVPRDPEEVLNDAKDFLGQYFASIRRANTPAHEARWKIVQEEVATTGTYQLTTTELVFGAKLAWRNASRCIGRIQWSKLQVFDCRQVTTTSGMFEALCNHIKYSTNKGNIRSAITLFPQRTDGKHDYRIWNSQLISYAGYRQPDGTVLGDPMHCEFTDLCLKLGWKPPRTAWDILPLVLSANGKDPDYFEIPRELVMEIHMTHPTFEWFKELELRWYALPAVSSMRFDCGGIEFTANAFNGWYMSTEIGCRNFCDTNRLNVLEKIAQNMGLDTRTPVNLWKDKALVEVNVAVLHSFQQHNATIVDHHTASESFIKHLDNENRLRSGCPADWIWIVPPMSSSITPVFHQEMALYYLKPSYEYQEPAWKTHQWQKSKPITGKRPINRKFHFKQIARAVKFTSKLFGRALSKRIKATVLYATETGKSEQYAKELGVIFGHAFNAQVHCMADYDITSIEHEALLLVVTSTFGNGDPPENGVAFGEHLCEILYADQVGEDSTGNQMLTPKSFIKANSDIQRYTAGNPKKLNRLESLKGSTTDATSIDSFGPLSNVRFAVFALGSSAYPNFCNFGKYVDKLLVDLGGERIHDLATGDEMCGQDQAFRKWASSVFNVACETFCLDDDETLQEAKRALGTVALSEETVQFARAGCRPATLHAALQKSLNKQFVSCTVKANKDLGDASAERSTIFIDLEPKEEIKYNPGDHVGIIACNRKELVESLLSRIKDVDDYDEPLQLQLLKETHTSSGLVKSWEPHEKLPIMSVRELFTRFLDITTPPTTILLQYLATTCEDEEEKKQLNVLATDPGAYEDWRHFHFPTLPEVLDQFPSARPNASLLAALLSPLQPRFYSISSSPLAHAKRLHLTVAVVTYRTQDGEGPVHYGVCSNYLMERKPGDEVYLFIRSAPNFHLPQDLSVPLILIGPGTGIAPFRGFWHHRRALQNSCSRTTTGPVWLFFGCRTKTMDLYREEKEQALKEGVLSKVFLALSREKEVPKTYVQEVAENVGAEIHDLLINKGAHFYVCGDCKMAEDVHQKLKGIVKKHGNMTDEQVQNFMFMLKEENRYHEDIFGITLRTAEVHSASRESARRNRVASQP

Target ORF information:

RefSeq Version	XM_021347365.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021347365.1

ORF Insert Sequence:

ATGATGTCCC AACATCTGAC GAGCATGTTC GAACAGCTCG GGAACTTTAC GAAGAACAAC 
TTGACGCACA ACAGCAAGCT GGTTAAAGAG AGAGAGAATA AGTCGAGTGC TAACCACGTG 
GGCGCCGGTC CAGGCGGTGG CGGGAACCAA AACCCGTCTC CTTCGCACAA GCACGTGCTG 
GCACAGCCTT CGCTACCGCT GAAGAAGCCC TGCAAGCTAA GGAACGTCGC ATCGAAAGCA 
GAGTCTTACG ACACTCTGTA TGCGAACAGC TCACTGCCGA CGCTATGCAA CGAGCGCTCG 
TGTCTTGGGA GTTTGATGGC CATCCCGGGA CGAGGCGACG TGCCCCGAGA CCCGGAGGAG 
GTGCTTAATG ACGCAAAGGA CTTTCTAGGC CAGTACTTCG CTTCTATACG GAGGGCCAAT 
ACTCCAGCTC ATGAAGCTCG CTGGAAAATC GTACAAGAGG AAGTAGCTAC GACTGGCACA 
TACCAACTAA CCACAACAGA ACTGGTATTT GGAGCCAAGC TTGCATGGAG AAACGCTAGC 
CGTTGCATTG GCAGAATCCA GTGGTCAAAA TTGCAGGTAT TCGATTGTCG CCAAGTCACA 
ACAACTAGTG GAATGTTCGA AGCACTCTGT AACCACATCA AGTACAGCAC AAATAAAGGA 
AATATAAGAT CAGCTATAAC ATTGTTCCCA CAACGTACGG ACGGCAAGCA CGACTACAGA 
ATATGGAACA GCCAGCTGAT CAGCTACGCT GGCTACCGTC AGCCAGACGG TACGGTGCTT 
GGTGACCCCA TGCACTGCGA ATTCACTGAT CTCTGCTTGA AACTTGGCTG GAAGCCGCCA 
CGCACGGCCT GGGATATTCT TCCCCTAGTT CTGTCAGCTA ACGGGAAAGA CCCTGACTAC 
TTCGAAATCC CCAGGGAGCT TGTTATGGAA ATCCACATGA CGCACCCCAC GTTCGAATGG 
TTCAAAGAAC TAGAATTACG TTGGTACGCT TTGCCCGCCG TCTCCAGTAT GCGTTTTGAT 
TGCGGTGGCA TCGAATTCAC GGCCAACGCT TTCAACGGTT GGTACATGAG CACTGAAATC 
GGCTGCAGGA ACTTCTGTGA CACCAACCGG CTTAATGTTT TGGAGAAAAT AGCTCAAAAC 
ATGGGCCTAG ATACCAGAAC TCCGGTCAAT CTTTGGAAGG ATAAGGCCCT CGTGGAAGTG 
AACGTGGCTG TACTGCACAG CTTTCAACAG CACAACGCGA CTATCGTGGA TCATCATACA 
GCTTCTGAAA GCTTCATAAA GCATTTGGAT AACGAGAACC GGCTTCGGTC TGGATGTCCA 
GCCGATTGGA TATGGATCGT ACCTCCCATG TCATCGTCCA TAACCCCGGT GTTTCACCAA 
GAAATGGCGC TCTACTACTT GAAACCTTCA TACGAATATC AGGAGCCTGC ATGGAAGACG 
CATCAGTGGC AGAAATCTAA GCCGATCACA GGCAAAAGAC CCATAAACAG AAAGTTCCAT 
TTCAAGCAAA TTGCCCGAGC TGTCAAATTC ACTTCGAAAT TGTTCGGTCG TGCCCTTTCG 
AAGAGGATTA AAGCGACTGT GCTGTACGCT ACGGAGACTG GGAAATCGGA ACAATACGCC 
AAAGAGCTTG GTGTTATATT TGGACACGCC TTTAATGCGC AGGTTCATTG TATGGCGGAT 
TACGATATAA CTTCAATTGA ACACGAAGCT CTCCTTCTGG TTGTGACATC GACGTTTGGA 
AATGGTGACC CACCCGAGAA TGGTGTCGCA TTTGGAGAGC ATCTATGCGA AATATTATAC 
GCTGATCAAG TCGGTGAAGA TTCTACTGGA AATCAAATGT TGACACCAAA ATCGTTCATA 
AAAGCCAATA GTGACATTCA AAGGTATACA GCAGGAAATC CGAAAAAACT CAACAGACTC 
GAATCATTGA AGGGTAGTAC AACAGACGCC ACATCCATTG ACAGCTTTGG TCCATTGAGT 
AATGTTAGAT TTGCCGTGTT CGCTCTGGGT TCGAGCGCTT ACCCGAATTT CTGTAACTTC 
GGAAAGTATG TAGACAAATT GTTGGTTGAC CTCGGCGGGG AACGAATCCA CGACCTCGCA 
ACCGGTGACG AAATGTGCGG CCAAGATCAG GCCTTCCGGA AGTGGGCCTC TAGCGTCTTC 
AACGTTGCTT GTGAGACATT CTGCTTGGAT GATGATGAGA CTCTACAAGA GGCAAAGAGA 
GCTCTCGGTA CTGTTGCATT AAGCGAGGAG ACAGTTCAAT TCGCCAGGGC TGGCTGTCGT 
CCCGCTACGT TACACGCAGC GCTACAAAAG TCTTTGAATA AACAATTCGT TTCTTGCACC 
GTCAAAGCTA ATAAAGATTT AGGCGATGCT TCCGCTGAAA GATCCACCAT CTTTATTGAT 
CTGGAACCTA AGGAGGAAAT AAAGTATAAT CCAGGAGATC ATGTCGGAAT AATAGCTTGT 
AACCGTAAAG AGCTCGTTGA GAGTCTGCTC TCCCGCATTA AAGACGTTGA TGATTACGAC 
GAGCCGTTGC AGTTGCAGCT TCTGAAAGAA ACTCATACGT CAAGCGGTTT AGTCAAATCT 
TGGGAGCCCC ACGAGAAACT TCCGATAATG AGCGTGCGTG AATTGTTCAC ACGATTCCTT 
GATATAACAA CTCCACCGAC CACGATACTT CTTCAGTACT TGGCCACAAC GTGCGAAGAT 
GAAGAAGAAA AAAAACAATT GAATGTTTTG GCTACGGATC CCGGAGCTTA CGAAGATTGG 
CGTCATTTCC ACTTCCCCAC CTTGCCCGAA GTCCTAGATC AGTTTCCATC AGCAAGACCC 
AATGCATCTT TACTTGCCGC CCTTTTATCC CCACTGCAGC CAAGATTTTA CTCAATTTCT 
TCGTCACCAT TAGCCCACGC AAAGAGATTG CATCTTACGG TTGCCGTAGT CACATATCGA 
ACTCAAGATG GTGAGGGACC GGTCCATTAT GGTGTGTGTT CCAACTACTT AATGGAACGT 
AAGCCTGGCG ATGAGGTTTA CCTCTTTATC AGAAGTGCTC CTAATTTCCA TTTACCTCAA 
GATTTGTCAG TTCCTCTTAT ACTAATTGGT CCCGGTACTG GTATAGCTCC ATTCCGTGGC 
TTTTGGCATC ATCGGCGAGC TCTACAAAAT TCTTGCTCGC GTACAACCAC TGGACCGGTG 
TGGCTGTTCT TCGGCTGTCG AACGAAGACT ATGGACTTAT ACCGAGAAGA GAAAGAGCAA 
GCTTTGAAAG AAGGCGTACT TTCTAAAGTA TTTCTCGCTC TATCTAGAGA AAAGGAAGTT 
CCAAAAACGT ACGTCCAAGA AGTTGCTGAA AACGTTGGTG CAGAAATACA CGATTTACTA 
ATTAATAAAG GTGCACACTT TTACGTTTGC GGTGATTGTA AAATGGCGGA GGATGTGCAC 
CAGAAGCTCA AAGGTATCGT GAAGAAACAC GGAAACATGA CAGACGAACA AGTACAGAAC 
TTTATGTTCA TGCTAAAGGA AGAGAATCGT TACCACGAAG ACATATTCGG CATCACACTG 
CGCACAGCTG AGGTCCACAG CGCTTCTCGC GAGTCGGCTC GTAGGAACCG CGTCGCTTCC 
CAACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( XM_021347397.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_021203072.1 Bombyx mori nitric oxide synthase 2 (NOS2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBg22348
Clone ID Related Accession (Same CDS sequence)	XM_021347365.1 , XM_021347397.1
Accession Version	XM_021347397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1496505600000
Organism	Bombyx mori(domestic silkworm)
Product	nitric oxide synthase-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004581679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCC AACATCTGAC GAGCATGTTC GAACAGCTCG GGAACTTTAC GAAGAACAAC 
TTGACGCACA ACAGCAAGCT GGTTAAAGAG AGAGAGAATA AGTCGAGTGC TAACCACGTG 
GGCGCCGGTC CAGGCGGTGG CGGGAACCAA AACCCGTCTC CTTCGCACAA GCACGTGCTG 
GCACAGCCTT CGCTACCGCT GAAGAAGCCC TGCAAGCTAA GGAACGTCGC ATCGAAAGCA 
GAGTCTTACG ACACTCTGTA TGCGAACAGC TCACTGCCGA CGCTATGCAA CGAGCGCTCG 
TGTCTTGGGA GTTTGATGGC CATCCCGGGA CGAGGCGACG TGCCCCGAGA CCCGGAGGAG 
GTGCTTAATG ACGCAAAGGA CTTTCTAGGC CAGTACTTCG CTTCTATACG GAGGGCCAAT 
ACTCCAGCTC ATGAAGCTCG CTGGAAAATC GTACAAGAGG AAGTAGCTAC GACTGGCACA 
TACCAACTAA CCACAACAGA ACTGGTATTT GGAGCCAAGC TTGCATGGAG AAACGCTAGC 
CGTTGCATTG GCAGAATCCA GTGGTCAAAA TTGCAGGTAT TCGATTGTCG CCAAGTCACA 
ACAACTAGTG GAATGTTCGA AGCACTCTGT AACCACATCA AGTACAGCAC AAATAAAGGA 
AATATAAGAT CAGCTATAAC ATTGTTCCCA CAACGTACGG ACGGCAAGCA CGACTACAGA 
ATATGGAACA GCCAGCTGAT CAGCTACGCT GGCTACCGTC AGCCAGACGG TACGGTGCTT 
GGTGACCCCA TGCACTGCGA ATTCACTGAT CTCTGCTTGA AACTTGGCTG GAAGCCGCCA 
CGCACGGCCT GGGATATTCT TCCCCTAGTT CTGTCAGCTA ACGGGAAAGA CCCTGACTAC 
TTCGAAATCC CCAGGGAGCT TGTTATGGAA ATCCACATGA CGCACCCCAC GTTCGAATGG 
TTCAAAGAAC TAGAATTACG TTGGTACGCT TTGCCCGCCG TCTCCAGTAT GCGTTTTGAT 
TGCGGTGGCA TCGAATTCAC GGCCAACGCT TTCAACGGTT GGTACATGAG CACTGAAATC 
GGCTGCAGGA ACTTCTGTGA CACCAACCGG CTTAATGTTT TGGAGAAAAT AGCTCAAAAC 
ATGGGCCTAG ATACCAGAAC TCCGGTCAAT CTTTGGAAGG ATAAGGCCCT CGTGGAAGTG 
AACGTGGCTG TACTGCACAG CTTTCAACAG CACAACGCGA CTATCGTGGA TCATCATACA 
GCTTCTGAAA GCTTCATAAA GCATTTGGAT AACGAGAACC GGCTTCGGTC TGGATGTCCA 
GCCGATTGGA TATGGATCGT ACCTCCCATG TCATCGTCCA TAACCCCGGT GTTTCACCAA 
GAAATGGCGC TCTACTACTT GAAACCTTCA TACGAATATC AGGAGCCTGC ATGGAAGACG 
CATCAGTGGC AGAAATCTAA GCCGATCACA GGCAAAAGAC CCATAAACAG AAAGTTCCAT 
TTCAAGCAAA TTGCCCGAGC TGTCAAATTC ACTTCGAAAT TGTTCGGTCG TGCCCTTTCG 
AAGAGGATTA AAGCGACTGT GCTGTACGCT ACGGAGACTG GGAAATCGGA ACAATACGCC 
AAAGAGCTTG GTGTTATATT TGGACACGCC TTTAATGCGC AGGTTCATTG TATGGCGGAT 
TACGATATAA CTTCAATTGA ACACGAAGCT CTCCTTCTGG TTGTGACATC GACGTTTGGA 
AATGGTGACC CACCCGAGAA TGGTGTCGCA TTTGGAGAGC ATCTATGCGA AATATTATAC 
GCTGATCAAG TCGGTGAAGA TTCTACTGGA AATCAAATGT TGACACCAAA ATCGTTCATA 
AAAGCCAATA GTGACATTCA AAGGTATACA GCAGGAAATC CGAAAAAACT CAACAGACTC 
GAATCATTGA AGGGTAGTAC AACAGACGCC ACATCCATTG ACAGCTTTGG TCCATTGAGT 
AATGTTAGAT TTGCCGTGTT CGCTCTGGGT TCGAGCGCTT ACCCGAATTT CTGTAACTTC 
GGAAAGTATG TAGACAAATT GTTGGTTGAC CTCGGCGGGG AACGAATCCA CGACCTCGCA 
ACCGGTGACG AAATGTGCGG CCAAGATCAG GCCTTCCGGA AGTGGGCCTC TAGCGTCTTC 
AACGTTGCTT GTGAGACATT CTGCTTGGAT GATGATGAGA CTCTACAAGA GGCAAAGAGA 
GCTCTCGGTA CTGTTGCATT AAGCGAGGAG ACAGTTCAAT TCGCCAGGGC TGGCTGTCGT 
CCCGCTACGT TACACGCAGC GCTACAAAAG TCTTTGAATA AACAATTCGT TTCTTGCACC 
GTCAAAGCTA ATAAAGATTT AGGCGATGCT TCCGCTGAAA GATCCACCAT CTTTATTGAT 
CTGGAACCTA AGGAGGAAAT AAAGTATAAT CCAGGAGATC ATGTCGGAAT AATAGCTTGT 
AACCGTAAAG AGCTCGTTGA GAGTCTGCTC TCCCGCATTA AAGACGTTGA TGATTACGAC 
GAGCCGTTGC AGTTGCAGCT TCTGAAAGAA ACTCATACGT CAAGCGGTTT AGTCAAATCT 
TGGGAGCCCC ACGAGAAACT TCCGATAATG AGCGTGCGTG AATTGTTCAC ACGATTCCTT 
GATATAACAA CTCCACCGAC CACGATACTT CTTCAGTACT TGGCCACAAC GTGCGAAGAT 
GAAGAAGAAA AAAAACAATT GAATGTTTTG GCTACGGATC CCGGAGCTTA CGAAGATTGG 
CGTCATTTCC ACTTCCCCAC CTTGCCCGAA GTCCTAGATC AGTTTCCATC AGCAAGACCC 
AATGCATCTT TACTTGCCGC CCTTTTATCC CCACTGCAGC CAAGATTTTA CTCAATTTCT 
TCGTCACCAT TAGCCCACGC AAAGAGATTG CATCTTACGG TTGCCGTAGT CACATATCGA 
ACTCAAGATG GTGAGGGACC GGTCCATTAT GGTGTGTGTT CCAACTACTT AATGGAACGT 
AAGCCTGGCG ATGAGGTTTA CCTCTTTATC AGAAGTGCTC CTAATTTCCA TTTACCTCAA 
GATTTGTCAG TTCCTCTTAT ACTAATTGGT CCCGGTACTG GTATAGCTCC ATTCCGTGGC 
TTTTGGCATC ATCGGCGAGC TCTACAAAAT TCTTGCTCGC GTACAACCAC TGGACCGGTG 
TGGCTGTTCT TCGGCTGTCG AACGAAGACT ATGGACTTAT ACCGAGAAGA GAAAGAGCAA 
GCTTTGAAAG AAGGCGTACT TTCTAAAGTA TTTCTCGCTC TATCTAGAGA AAAGGAAGTT 
CCAAAAACGT ACGTCCAAGA AGTTGCTGAA AACGTTGGTG CAGAAATACA CGATTTACTA 
ATTAATAAAG GTGCACACTT TTACGTTTGC GGTGATTGTA AAATGGCGGA GGATGTGCAC 
CAGAAGCTCA AAGGTATCGT GAAGAAACAC GGAAACATGA CAGACGAACA AGTACAGAAC 
TTTATGTTCA TGCTAAAGGA AGAGAATCGT TACCACGAAG ACATATTCGG CATCACACTG 
CGCACAGCTG AGGTCCACAG CGCTTCTCGC GAGTCGGCTC GTAGGAACCG CGTCGCTTCC 
CAACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021203072.1
CDS	158..3766
Translation	MMSQHLTSMFEQLGNFTKNNLTHNSKLVKERENKSSANHVGAGPGGGGNQNPSPSHKHVLAQPSLPLKKPCKLRNVASKAESYDTLYANSSLPTLCNERSCLGSLMAIPGRGDVPRDPEEVLNDAKDFLGQYFASIRRANTPAHEARWKIVQEEVATTGTYQLTTTELVFGAKLAWRNASRCIGRIQWSKLQVFDCRQVTTTSGMFEALCNHIKYSTNKGNIRSAITLFPQRTDGKHDYRIWNSQLISYAGYRQPDGTVLGDPMHCEFTDLCLKLGWKPPRTAWDILPLVLSANGKDPDYFEIPRELVMEIHMTHPTFEWFKELELRWYALPAVSSMRFDCGGIEFTANAFNGWYMSTEIGCRNFCDTNRLNVLEKIAQNMGLDTRTPVNLWKDKALVEVNVAVLHSFQQHNATIVDHHTASESFIKHLDNENRLRSGCPADWIWIVPPMSSSITPVFHQEMALYYLKPSYEYQEPAWKTHQWQKSKPITGKRPINRKFHFKQIARAVKFTSKLFGRALSKRIKATVLYATETGKSEQYAKELGVIFGHAFNAQVHCMADYDITSIEHEALLLVVTSTFGNGDPPENGVAFGEHLCEILYADQVGEDSTGNQMLTPKSFIKANSDIQRYTAGNPKKLNRLESLKGSTTDATSIDSFGPLSNVRFAVFALGSSAYPNFCNFGKYVDKLLVDLGGERIHDLATGDEMCGQDQAFRKWASSVFNVACETFCLDDDETLQEAKRALGTVALSEETVQFARAGCRPATLHAALQKSLNKQFVSCTVKANKDLGDASAERSTIFIDLEPKEEIKYNPGDHVGIIACNRKELVESLLSRIKDVDDYDEPLQLQLLKETHTSSGLVKSWEPHEKLPIMSVRELFTRFLDITTPPTTILLQYLATTCEDEEEKKQLNVLATDPGAYEDWRHFHFPTLPEVLDQFPSARPNASLLAALLSPLQPRFYSISSSPLAHAKRLHLTVAVVTYRTQDGEGPVHYGVCSNYLMERKPGDEVYLFIRSAPNFHLPQDLSVPLILIGPGTGIAPFRGFWHHRRALQNSCSRTTTGPVWLFFGCRTKTMDLYREEKEQALKEGVLSKVFLALSREKEVPKTYVQEVAENVGAEIHDLLINKGAHFYVCGDCKMAEDVHQKLKGIVKKHGNMTDEQVQNFMFMLKEENRYHEDIFGITLRTAEVHSASRESARRNRVASQP

Target ORF information:

RefSeq Version	XM_021347397.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori nitric oxide synthase 2 (NOS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021347397.1

ORF Insert Sequence:

ATGATGTCCC AACATCTGAC GAGCATGTTC GAACAGCTCG GGAACTTTAC GAAGAACAAC 
TTGACGCACA ACAGCAAGCT GGTTAAAGAG AGAGAGAATA AGTCGAGTGC TAACCACGTG 
GGCGCCGGTC CAGGCGGTGG CGGGAACCAA AACCCGTCTC CTTCGCACAA GCACGTGCTG 
GCACAGCCTT CGCTACCGCT GAAGAAGCCC TGCAAGCTAA GGAACGTCGC ATCGAAAGCA 
GAGTCTTACG ACACTCTGTA TGCGAACAGC TCACTGCCGA CGCTATGCAA CGAGCGCTCG 
TGTCTTGGGA GTTTGATGGC CATCCCGGGA CGAGGCGACG TGCCCCGAGA CCCGGAGGAG 
GTGCTTAATG ACGCAAAGGA CTTTCTAGGC CAGTACTTCG CTTCTATACG GAGGGCCAAT 
ACTCCAGCTC ATGAAGCTCG CTGGAAAATC GTACAAGAGG AAGTAGCTAC GACTGGCACA 
TACCAACTAA CCACAACAGA ACTGGTATTT GGAGCCAAGC TTGCATGGAG AAACGCTAGC 
CGTTGCATTG GCAGAATCCA GTGGTCAAAA TTGCAGGTAT TCGATTGTCG CCAAGTCACA 
ACAACTAGTG GAATGTTCGA AGCACTCTGT AACCACATCA AGTACAGCAC AAATAAAGGA 
AATATAAGAT CAGCTATAAC ATTGTTCCCA CAACGTACGG ACGGCAAGCA CGACTACAGA 
ATATGGAACA GCCAGCTGAT CAGCTACGCT GGCTACCGTC AGCCAGACGG TACGGTGCTT 
GGTGACCCCA TGCACTGCGA ATTCACTGAT CTCTGCTTGA AACTTGGCTG GAAGCCGCCA 
CGCACGGCCT GGGATATTCT TCCCCTAGTT CTGTCAGCTA ACGGGAAAGA CCCTGACTAC 
TTCGAAATCC CCAGGGAGCT TGTTATGGAA ATCCACATGA CGCACCCCAC GTTCGAATGG 
TTCAAAGAAC TAGAATTACG TTGGTACGCT TTGCCCGCCG TCTCCAGTAT GCGTTTTGAT 
TGCGGTGGCA TCGAATTCAC GGCCAACGCT TTCAACGGTT GGTACATGAG CACTGAAATC 
GGCTGCAGGA ACTTCTGTGA CACCAACCGG CTTAATGTTT TGGAGAAAAT AGCTCAAAAC 
ATGGGCCTAG ATACCAGAAC TCCGGTCAAT CTTTGGAAGG ATAAGGCCCT CGTGGAAGTG 
AACGTGGCTG TACTGCACAG CTTTCAACAG CACAACGCGA CTATCGTGGA TCATCATACA 
GCTTCTGAAA GCTTCATAAA GCATTTGGAT AACGAGAACC GGCTTCGGTC TGGATGTCCA 
GCCGATTGGA TATGGATCGT ACCTCCCATG TCATCGTCCA TAACCCCGGT GTTTCACCAA 
GAAATGGCGC TCTACTACTT GAAACCTTCA TACGAATATC AGGAGCCTGC ATGGAAGACG 
CATCAGTGGC AGAAATCTAA GCCGATCACA GGCAAAAGAC CCATAAACAG AAAGTTCCAT 
TTCAAGCAAA TTGCCCGAGC TGTCAAATTC ACTTCGAAAT TGTTCGGTCG TGCCCTTTCG 
AAGAGGATTA AAGCGACTGT GCTGTACGCT ACGGAGACTG GGAAATCGGA ACAATACGCC 
AAAGAGCTTG GTGTTATATT TGGACACGCC TTTAATGCGC AGGTTCATTG TATGGCGGAT 
TACGATATAA CTTCAATTGA ACACGAAGCT CTCCTTCTGG TTGTGACATC GACGTTTGGA 
AATGGTGACC CACCCGAGAA TGGTGTCGCA TTTGGAGAGC ATCTATGCGA AATATTATAC 
GCTGATCAAG TCGGTGAAGA TTCTACTGGA AATCAAATGT TGACACCAAA ATCGTTCATA 
AAAGCCAATA GTGACATTCA AAGGTATACA GCAGGAAATC CGAAAAAACT CAACAGACTC 
GAATCATTGA AGGGTAGTAC AACAGACGCC ACATCCATTG ACAGCTTTGG TCCATTGAGT 
AATGTTAGAT TTGCCGTGTT CGCTCTGGGT TCGAGCGCTT ACCCGAATTT CTGTAACTTC 
GGAAAGTATG TAGACAAATT GTTGGTTGAC CTCGGCGGGG AACGAATCCA CGACCTCGCA 
ACCGGTGACG AAATGTGCGG CCAAGATCAG GCCTTCCGGA AGTGGGCCTC TAGCGTCTTC 
AACGTTGCTT GTGAGACATT CTGCTTGGAT GATGATGAGA CTCTACAAGA GGCAAAGAGA 
GCTCTCGGTA CTGTTGCATT AAGCGAGGAG ACAGTTCAAT TCGCCAGGGC TGGCTGTCGT 
CCCGCTACGT TACACGCAGC GCTACAAAAG TCTTTGAATA AACAATTCGT TTCTTGCACC 
GTCAAAGCTA ATAAAGATTT AGGCGATGCT TCCGCTGAAA GATCCACCAT CTTTATTGAT 
CTGGAACCTA AGGAGGAAAT AAAGTATAAT CCAGGAGATC ATGTCGGAAT AATAGCTTGT 
AACCGTAAAG AGCTCGTTGA GAGTCTGCTC TCCCGCATTA AAGACGTTGA TGATTACGAC 
GAGCCGTTGC AGTTGCAGCT TCTGAAAGAA ACTCATACGT CAAGCGGTTT AGTCAAATCT 
TGGGAGCCCC ACGAGAAACT TCCGATAATG AGCGTGCGTG AATTGTTCAC ACGATTCCTT 
GATATAACAA CTCCACCGAC CACGATACTT CTTCAGTACT TGGCCACAAC GTGCGAAGAT 
GAAGAAGAAA AAAAACAATT GAATGTTTTG GCTACGGATC CCGGAGCTTA CGAAGATTGG 
CGTCATTTCC ACTTCCCCAC CTTGCCCGAA GTCCTAGATC AGTTTCCATC AGCAAGACCC 
AATGCATCTT TACTTGCCGC CCTTTTATCC CCACTGCAGC CAAGATTTTA CTCAATTTCT 
TCGTCACCAT TAGCCCACGC AAAGAGATTG CATCTTACGG TTGCCGTAGT CACATATCGA 
ACTCAAGATG GTGAGGGACC GGTCCATTAT GGTGTGTGTT CCAACTACTT AATGGAACGT 
AAGCCTGGCG ATGAGGTTTA CCTCTTTATC AGAAGTGCTC CTAATTTCCA TTTACCTCAA 
GATTTGTCAG TTCCTCTTAT ACTAATTGGT CCCGGTACTG GTATAGCTCC ATTCCGTGGC 
TTTTGGCATC ATCGGCGAGC TCTACAAAAT TCTTGCTCGC GTACAACCAC TGGACCGGTG 
TGGCTGTTCT TCGGCTGTCG AACGAAGACT ATGGACTTAT ACCGAGAAGA GAAAGAGCAA 
GCTTTGAAAG AAGGCGTACT TTCTAAAGTA TTTCTCGCTC TATCTAGAGA AAAGGAAGTT 
CCAAAAACGT ACGTCCAAGA AGTTGCTGAA AACGTTGGTG CAGAAATACA CGATTTACTA 
ATTAATAAAG GTGCACACTT TTACGTTTGC GGTGATTGTA AAATGGCGGA GGATGTGCAC 
CAGAAGCTCA AAGGTATCGT GAAGAAACAC GGAAACATGA CAGACGAACA AGTACAGAAC 
TTTATGTTCA TGCTAAAGGA AGAGAATCGT TACCACGAAG ACATATTCGG CATCACACTG 
CGCACAGCTG AGGTCCACAG CGCTTCTCGC GAGTCGGCTC GTAGGAACCG CGTCGCTTCC 
CAACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Success in the acquisition of Bombyx mori sperm motility is influenced by the extracellular production of nitric oxide (NO) in the presence of seminal fluid nitric oxide synthase (NOS).
Insect biochemistry and molecular biology8440-47(2017 05)
Nagaoka S,Asagoshi M,Kato K,Takata Y

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
BMC genomics15820(2014 Sep)
Ou J,Deng HM,Zheng SC,Huang LH,Feng QL,Liu L

Large scale full-length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori.
G3 (Bethesda, Md.)3(9)1481-92(2013 Sep)
Suetsugu Y,Futahashi R,Kanamori H,Kadono-Okuda K,Sasanuma S,Narukawa J,Ajimura M,Jouraku A,Namiki N,Shimomura M,Sezutsu H,Osanai-Futahashi M,Suzuki MG,Daimon T,Shinoda T,Taniai K,Asaoka K,Niwa R,Kawaoka S,Katsuma S,Tamura T,Noda H,Kasahara M,Sugano S,Suzuki Y,Fujiwara H,Kataoka H,Arunkumar KP,Tomar A,Nagaraju J,Goldsmith MR,Feng Q,Xia Q,Yamamoto K,Shimada T,Mita K

A genome-wide analysis of genes and gene families involved in innate immunity of Bombyx mori.
Insect biochemistry and molecular biology38(12)1087-110(2008 Dec)
Tanaka H,Ishibashi J,Fujita K,Nakajima Y,Sagisaka A,Tomimoto K,Suzuki N,Yoshiyama M,Kaneko Y,Iwasaki T,Sunagawa T,Yamaji K,Asaoka A,Mita K,Yamakawa M

Positional cloning of a Bombyx wingless locus flugellos (fl) reveals a crucial role for fringe that is specific for wing morphogenesis.
Genetics179(2)875-85(2008 Jun)
Sato K,Matsunaga TM,Futahashi R,Kojima T,Mita K,Banno Y,Fujiwara H

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NOS2 cDNA ORF clone, Bombyx mori(domestic silkworm)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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