cenpj cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene
Clones
gRNAs

Summary

Gene Symbol	cenpj
Entrez Gene ID	103031572
Full Name	centromere protein J
Gene Type	protein-coding
Organism	Astyanax mexicanus(Mexican tetra)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007260586.3	XP_007260648.2	centromere protein J isoform X2
XM_007260586.2	XP_007260648.1	centromere protein J isoform X2
XM_007260585.2	XP_007260647.1	centromere protein J isoform X1
XM_015608668.1	XP_015464154.1	centromere protein J isoform X3
XM_007260585.3	XP_007260647.2	centromere protein J isoform X1

Pathways from BioSystems
Gene Ontology

The following cenpj gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cenpj cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAs114116	XM_007260585.3 Latest version!	Astyanax mexicanus centromere protein J (cenpj), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAs114117	XM_007260586.3 Latest version!	Astyanax mexicanus centromere protein J (cenpj), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAs42146	XM_007260585.2 Latest version!	Astyanax mexicanus centromere protein J (cenpj), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAs42147	XM_007260586.2 Latest version!	Astyanax mexicanus centromere protein J (cenpj), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAs42148	XM_015608668.1 Latest version!	Astyanax mexicanus centromere protein J (cenpj), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cenpj ( XM_007260585.3 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007260647.2 Astyanax mexicanus centromere protein J (cenpj), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAs114116
Clone ID Related Accession (Same CDS sequence)	XM_007260585.3
Accession Version	XM_007260585.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	centromere protein J isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035915.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 3, 2017 this sequence version replaced XM_007260585.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCTC CAGCTGGACC ACAGCCATCA GAGTCTCGCT TCCTGTCCCG CTGGATGCCC 
AGCAGCTCTC GGGCCGGGGT CTTCCTGAGT GGCTCTCCAG GAGAGGTCAG CTCTGTCCAG 
AGCAGCTGCT CTTCTCTGGC TCAGGAGATC TCTATGGAAG ACTCTTTCTG CTCCCAGTTC 
GCCCCTCTGC CTGTGTCCTG CAGCAGCAGC CTGGATTCAT TGGTGCCAAA GGTGTCCGAA 
ATAGACCCGC AGAGAAAAGC AACAAGCAGC AACCCAGACA GTGTCCAGGA CATTTTAACA 
AGAGTACAGG AACTTCCATT GCAGGCGAGG CTTGAGCAGC TAAAGCAGTT ACAGCAGCGC 
ATGCAGGAGC AGCTAAAAGC CCATCAGTTA GAGCAGCTGC AAAAACTGCA CAATGAACAG 
CAGGGCATTG TGGGGATGGT ACAGACAGGC GCAGCAGGAT ACACCCAAGA AAAACAGCAG 
TACAGCAGAA GCACATCATC TACACTTTCC ACTGCACAAC ACAGTGCTTT GCCTACACAC 
CAGGAAGAAG TGCTGAGTGA ACTTGAGTCA AGTGTGTGGA ATCATGAATC GAGTGTGGTG 
CAGTCGAGCT TTGGGCAAGA TGGTGATGGA GATGAAGATG ATATGATCGA AGGTTCACTG 
CAGTCTGTAA TTGAGAATCC AAGCATGTGC AGACAAGATA GGGAATCAGA GCCTCAAGAC 
AGGCCACTCA AATCAGCTAC CAGTGGGAAG ACATTTGAAG AGATGTTGGA AGAACAGCTG 
AAGTTGGAGA ATGAGAAGAT AAACAAGAAC GCTCCTGCTG AACCAGCAAA GGTCAAGAGG 
CCGTTTCTGA GACGTGGGGA AGGTCTGGCC AGGTTTAAAA GAGATAAAGC CTCCGTAACT 
TTCAAAGAAG GAACAGCTCG GCCAAGCACC AAACCCACTT CTTGCCCGAA TCCTGTGAAG 
TCTCAGGGCC AAGCACTTGC ATCCCGTCAC CAAAACTCAG AGCCAAAAAG ACCTTCGCCT 
AAACCTACAC CAACTAATAC TGTAATCCAA CGCAAGACTG CAGTTCTGAA CAAAGAGAAC 
TTGCCTCAGA GCAAGTCTTT ACTACCAGCA AGCAAAACTG TTGTCAAAGC TCATATCCTT 
GGATCCCACC AGGGTCAGAA CATGGGCTCT GGAGCTGTCC AGCAACTTGG CAGGAGTGCT 
CGGAGGCCTG ATCCTCCGCA GAAGATTGTC TGTGATGCTG TGGAGGATGT AAATAAGGGG 
CCATCACCTG GGCATCTCCG GGATCCTAGT ACATGTGTGG CGGATAACTC CTTTGAAGTG 
TGGCTTGCAG AGCGCGGGGA TCGTTGGGAA AAGGATAATC AACGTGAGTG TGTGGAGCTT 
GGTGAGTTTG AGCTGCTGGA GAGAGCAGCG GATGAACTAT CATTCTCAAG CAACTCTTCC 
TTTATTTGTA CTCTTCTTCA GGGAGATCGT AGACGTCTGT CCTCAACGCC AGTCAAATCA 
CCTCCAAAAC CTAAGTTGCC TGGTCATATT CAAGCTGACT CTAGAAACCC AGTGCAGCCG 
AGCATTAGCA GTGTCCAGCA GGGTGTACCA GACATGCCTG TAGACAGTGT TGCAGTAAAC 
ATTAAGAAGA GTGTTTTGGT CAGCCAGTAT TCGGAGGAGG AAGATGAGGG TAGTAAAATA 
GATGATGATG AAGATTCTGA TGCTTCATTG TGTAGCACTA CAGAGTTCCA ACAAACTGCA 
TTTGGACCAG GTCAGCCTTT CAGCCACTGC TTTCAAATAT CTCAGGTCCC GCCCTACAAT 
AAAAGCTCCT ACCAGGACAG GGAGGAAGGA GCTGCCAGTC CACTCGAGGA AGAGGAAAGC 
AATGGAGATT CTACTATAGT GGATGCCAAG GATCACATTG ACTTTGATGA CGATGACACT 
TGGAATGAGC CTGAGGACTT GACCTGCATT TCCGCTAAGA TGGACGATCA TGCAGGCACG 
ACACTGAAGA GGAAGGTTGC CTTCTCAAAA GGACCAGAGC TTGACCCTTC TGCTGACAGC 
TCACTGGGCC GTGAACCAGA GCCTCTGCCC ACATGCCAGT TGGTGGCAAA GCTGTTCCCA 
GCCCTCAAGC CGAAGCCCTG CCCTCCAGCT CCTCCAGTTG CAAAGAATCC AGAAGCAACA 
TGCGACGAAC AAGGAGCAGC CCAGTCCCGC TTACTTCGTG AGCGTTTGGT GGAGCTGGAA 
ACAGAGATTG AGAGGTTCAA GACAGAAAAT GCGGTGTTGA GCAGACTCAG ACAGGAAAAC 
GAGGATGTCA GAGAGAACCT GAGGAAAGAG ACAGCCCAGT TTCAGCAGAA GATGGCAGAT 
GAACTTGCCA AATGGGAAGA GTTCAAGCGC GAGGAAAGCC GGAAGCTGCA GCGGGAAAAG 
AAGCTGTTTG AGAAGCATGC GGCAGCTGCC AGGGCGAGAC CTGACAAACA GGAGCGAGAT 
GAGATGCAGT CTCTAAAGCA GCAACTGAAC TCTCTGCAAG AGGATTTAAG AAAACGAGAA 
GCTCGCTGGT CCAGCACCCA TCAACGCCTC CGGCAGCAGG TAGACACCCT GAGTGCTGAG 
AACACGACTC TCCGAGGCCA GGTCCGCATG CTGGAGAAAC TCCGCCTGAC TGCTTGGAAA 
AATGCTGAGA GTGAGCGTGA AAAAGAGAAA GCGAGGGGAA GGTCTAATGC ACCTGGTGGT 
GGTGGTGGCA GCACCACCAG TGTCACAGCT CAGAGAAGGG CCAAATCCAA GAGTCCTTCC 
CACAGTGTAA AGACCAGCAG CACGAGCAGC AAAAGGGAAA GCCCAGAGCT TCAGAATCCT 
CATAAAATTC AAGCTTGTAA ATCAAGGTTT AAGCCTACTC TGAGTGAAAA GGAAGCCTGC 
CCGGTGGCTG ATCCATACCA CATATCTGAT GGACAACCTG AGCCTGAAGG GAGGAGCCAT 
GATTCTCTTC CAATCAATGA ATCCTCTTTA GAGGTGGAAA GTCCTAGAGC TGTTACAGAG 
CAGCTGGATA TGGAACAAGA AGAAATCACG CACACTGATG GCAAGATTGA AAAGCTTCTT 
CCAGATGGAG GGAGACTTAT AGTGTTCCCT AACGGCACAA GGAAAGAGCT GTCTGCAGAC 
GGGCTGTCGA TCAAAGTCAC TTTTTTTAAT GGAGACATCA AACACATCAT GCCTGATCAA 
AGAGTGATTT ATTACTATGC AGGAGCTCAG ACCACACATA CGACCTACCC TGATGGCATA 
GAAGTTCTCC AGTTCCCTAA TAATCAGATA GAAAAACACT TCCCTGATGG CCATAAGGAG 
ATCACCTTCC CTGATCAGAC AGTTAAGAAT ATCTACCCTG ATGGGAGAGA GGAGAGCGTA 
CTGGCAGACG GAACAATAAT ACAGCAAAAC CCGGATGGCA GTAAGAAGAT CCAGTTTAAC 
AACGGTCAGC GGGAACTTCA CACTGCTGAA TTCAAACGCC GAGAATATCC TGACGGTACG 
GTCAAAACAG TTTATTCAGA TGGCCGCCAG GAAACACGAT ATCCCAGTGG CCGCGTTCGA 
CTCAAAGACC CTCAGGGACG TGTTGTTATG GACGCCAAGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007260647.2
CDS	163..3747
Translation	MSSPAGPQPSESRFLSRWMPSSSRAGVFLSGSPGEVSSVQSSCSSLAQEISMEDSFCSQFAPLPVSCSSSLDSLVPKVSEIDPQRKATSSNPDSVQDILTRVQELPLQARLEQLKQLQQRMQEQLKAHQLEQLQKLHNEQQGIVGMVQTGAAGYTQEKQQYSRSTSSTLSTAQHSALPTHQEEVLSELESSVWNHESSVVQSSFGQDGDGDEDDMIEGSLQSVIENPSMCRQDRESEPQDRPLKSATSGKTFEEMLEEQLKLENEKINKNAPAEPAKVKRPFLRRGEGLARFKRDKASVTFKEGTARPSTKPTSCPNPVKSQGQALASRHQNSEPKRPSPKPTPTNTVIQRKTAVLNKENLPQSKSLLPASKTVVKAHILGSHQGQNMGSGAVQQLGRSARRPDPPQKIVCDAVEDVNKGPSPGHLRDPSTCVADNSFEVWLAERGDRWEKDNQRECVELGEFELLERAADELSFSSNSSFICTLLQGDRRRLSSTPVKSPPKPKLPGHIQADSRNPVQPSISSVQQGVPDMPVDSVAVNIKKSVLVSQYSEEEDEGSKIDDDEDSDASLCSTTEFQQTAFGPGQPFSHCFQISQVPPYNKSSYQDREEGAASPLEEEESNGDSTIVDAKDHIDFDDDDTWNEPEDLTCISAKMDDHAGTTLKRKVAFSKGPELDPSADSSLGREPEPLPTCQLVAKLFPALKPKPCPPAPPVAKNPEATCDEQGAAQSRLLRERLVELETEIERFKTENAVLSRLRQENEDVRENLRKETAQFQQKMADELAKWEEFKREESRKLQREKKLFEKHAAAARARPDKQERDEMQSLKQQLNSLQEDLRKREARWSSTHQRLRQQVDTLSAENTTLRGQVRMLEKLRLTAWKNAESEREKEKARGRSNAPGGGGGSTTSVTAQRRAKSKSPSHSVKTSSTSSKRESPELQNPHKIQACKSRFKPTLSEKEACPVADPYHISDGQPEPEGRSHDSLPINESSLEVESPRAVTEQLDMEQEEITHTDGKIEKLLPDGGRLIVFPNGTRKELSADGLSIKVTFFNGDIKHIMPDQRVIYYYAGAQTTHTTYPDGIEVLQFPNNQIEKHFPDGHKEITFPDQTVKNIYPDGREESVLADGTIIQQNPDGSKKIQFNNGQRELHTAEFKRREYPDGTVKTVYSDGRQETRYPSGRVRLKDPQGRVVMDAKP

Target ORF information:

RefSeq Version	XM_007260585.3
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus centromere protein J (cenpj), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007260585.3

ORF Insert Sequence:

ATGTCATCTC CAGCTGGACC ACAGCCATCA GAGTCTCGCT TCCTGTCCCG CTGGATGCCC 
AGCAGCTCTC GGGCCGGGGT CTTCCTGAGT GGCTCTCCAG GAGAGGTCAG CTCTGTCCAG 
AGCAGCTGCT CTTCTCTGGC TCAGGAGATC TCTATGGAAG ACTCTTTCTG CTCCCAGTTC 
GCCCCTCTGC CTGTGTCCTG CAGCAGCAGC CTGGATTCAT TGGTGCCAAA GGTGTCCGAA 
ATAGACCCGC AGAGAAAAGC AACAAGCAGC AACCCAGACA GTGTCCAGGA CATTTTAACA 
AGAGTACAGG AACTTCCATT GCAGGCGAGG CTTGAGCAGC TAAAGCAGTT ACAGCAGCGC 
ATGCAGGAGC AGCTAAAAGC CCATCAGTTA GAGCAGCTGC AAAAACTGCA CAATGAACAG 
CAGGGCATTG TGGGGATGGT ACAGACAGGC GCAGCAGGAT ACACCCAAGA AAAACAGCAG 
TACAGCAGAA GCACATCATC TACACTTTCC ACTGCACAAC ACAGTGCTTT GCCTACACAC 
CAGGAAGAAG TGCTGAGTGA ACTTGAGTCA AGTGTGTGGA ATCATGAATC GAGTGTGGTG 
CAGTCGAGCT TTGGGCAAGA TGGTGATGGA GATGAAGATG ATATGATCGA AGGTTCACTG 
CAGTCTGTAA TTGAGAATCC AAGCATGTGC AGACAAGATA GGGAATCAGA GCCTCAAGAC 
AGGCCACTCA AATCAGCTAC CAGTGGGAAG ACATTTGAAG AGATGTTGGA AGAACAGCTG 
AAGTTGGAGA ATGAGAAGAT AAACAAGAAC GCTCCTGCTG AACCAGCAAA GGTCAAGAGG 
CCGTTTCTGA GACGTGGGGA AGGTCTGGCC AGGTTTAAAA GAGATAAAGC CTCCGTAACT 
TTCAAAGAAG GAACAGCTCG GCCAAGCACC AAACCCACTT CTTGCCCGAA TCCTGTGAAG 
TCTCAGGGCC AAGCACTTGC ATCCCGTCAC CAAAACTCAG AGCCAAAAAG ACCTTCGCCT 
AAACCTACAC CAACTAATAC TGTAATCCAA CGCAAGACTG CAGTTCTGAA CAAAGAGAAC 
TTGCCTCAGA GCAAGTCTTT ACTACCAGCA AGCAAAACTG TTGTCAAAGC TCATATCCTT 
GGATCCCACC AGGGTCAGAA CATGGGCTCT GGAGCTGTCC AGCAACTTGG CAGGAGTGCT 
CGGAGGCCTG ATCCTCCGCA GAAGATTGTC TGTGATGCTG TGGAGGATGT AAATAAGGGG 
CCATCACCTG GGCATCTCCG GGATCCTAGT ACATGTGTGG CGGATAACTC CTTTGAAGTG 
TGGCTTGCAG AGCGCGGGGA TCGTTGGGAA AAGGATAATC AACGTGAGTG TGTGGAGCTT 
GGTGAGTTTG AGCTGCTGGA GAGAGCAGCG GATGAACTAT CATTCTCAAG CAACTCTTCC 
TTTATTTGTA CTCTTCTTCA GGGAGATCGT AGACGTCTGT CCTCAACGCC AGTCAAATCA 
CCTCCAAAAC CTAAGTTGCC TGGTCATATT CAAGCTGACT CTAGAAACCC AGTGCAGCCG 
AGCATTAGCA GTGTCCAGCA GGGTGTACCA GACATGCCTG TAGACAGTGT TGCAGTAAAC 
ATTAAGAAGA GTGTTTTGGT CAGCCAGTAT TCGGAGGAGG AAGATGAGGG TAGTAAAATA 
GATGATGATG AAGATTCTGA TGCTTCATTG TGTAGCACTA CAGAGTTCCA ACAAACTGCA 
TTTGGACCAG GTCAGCCTTT CAGCCACTGC TTTCAAATAT CTCAGGTCCC GCCCTACAAT 
AAAAGCTCCT ACCAGGACAG GGAGGAAGGA GCTGCCAGTC CACTCGAGGA AGAGGAAAGC 
AATGGAGATT CTACTATAGT GGATGCCAAG GATCACATTG ACTTTGATGA CGATGACACT 
TGGAATGAGC CTGAGGACTT GACCTGCATT TCCGCTAAGA TGGACGATCA TGCAGGCACG 
ACACTGAAGA GGAAGGTTGC CTTCTCAAAA GGACCAGAGC TTGACCCTTC TGCTGACAGC 
TCACTGGGCC GTGAACCAGA GCCTCTGCCC ACATGCCAGT TGGTGGCAAA GCTGTTCCCA 
GCCCTCAAGC CGAAGCCCTG CCCTCCAGCT CCTCCAGTTG CAAAGAATCC AGAAGCAACA 
TGCGACGAAC AAGGAGCAGC CCAGTCCCGC TTACTTCGTG AGCGTTTGGT GGAGCTGGAA 
ACAGAGATTG AGAGGTTCAA GACAGAAAAT GCGGTGTTGA GCAGACTCAG ACAGGAAAAC 
GAGGATGTCA GAGAGAACCT GAGGAAAGAG ACAGCCCAGT TTCAGCAGAA GATGGCAGAT 
GAACTTGCCA AATGGGAAGA GTTCAAGCGC GAGGAAAGCC GGAAGCTGCA GCGGGAAAAG 
AAGCTGTTTG AGAAGCATGC GGCAGCTGCC AGGGCGAGAC CTGACAAACA GGAGCGAGAT 
GAGATGCAGT CTCTAAAGCA GCAACTGAAC TCTCTGCAAG AGGATTTAAG AAAACGAGAA 
GCTCGCTGGT CCAGCACCCA TCAACGCCTC CGGCAGCAGG TAGACACCCT GAGTGCTGAG 
AACACGACTC TCCGAGGCCA GGTCCGCATG CTGGAGAAAC TCCGCCTGAC TGCTTGGAAA 
AATGCTGAGA GTGAGCGTGA AAAAGAGAAA GCGAGGGGAA GGTCTAATGC ACCTGGTGGT 
GGTGGTGGCA GCACCACCAG TGTCACAGCT CAGAGAAGGG CCAAATCCAA GAGTCCTTCC 
CACAGTGTAA AGACCAGCAG CACGAGCAGC AAAAGGGAAA GCCCAGAGCT TCAGAATCCT 
CATAAAATTC AAGCTTGTAA ATCAAGGTTT AAGCCTACTC TGAGTGAAAA GGAAGCCTGC 
CCGGTGGCTG ATCCATACCA CATATCTGAT GGACAACCTG AGCCTGAAGG GAGGAGCCAT 
GATTCTCTTC CAATCAATGA ATCCTCTTTA GAGGTGGAAA GTCCTAGAGC TGTTACAGAG 
CAGCTGGATA TGGAACAAGA AGAAATCACG CACACTGATG GCAAGATTGA AAAGCTTCTT 
CCAGATGGAG GGAGACTTAT AGTGTTCCCT AACGGCACAA GGAAAGAGCT GTCTGCAGAC 
GGGCTGTCGA TCAAAGTCAC TTTTTTTAAT GGAGACATCA AACACATCAT GCCTGATCAA 
AGAGTGATTT ATTACTATGC AGGAGCTCAG ACCACACATA CGACCTACCC TGATGGCATA 
GAAGTTCTCC AGTTCCCTAA TAATCAGATA GAAAAACACT TCCCTGATGG CCATAAGGAG 
ATCACCTTCC CTGATCAGAC AGTTAAGAAT ATCTACCCTG ATGGGAGAGA GGAGAGCGTA 
CTGGCAGACG GAACAATAAT ACAGCAAAAC CCGGATGGCA GTAAGAAGAT CCAGTTTAAC 
AACGGTCAGC GGGAACTTCA CACTGCTGAA TTCAAACGCC GAGAATATCC TGACGGTACG 
GTCAAAACAG TTTATTCAGA TGGCCGCCAG GAAACACGAT ATCCCAGTGG CCGCGTTCGA 
CTCAAAGACC CTCAGGGACG TGTTGTTATG GACGCCAAGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cenpj ( XM_007260586.3 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007260648.2 Astyanax mexicanus centromere protein J (cenpj), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAs114117
Clone ID Related Accession (Same CDS sequence)	XM_007260586.3
Accession Version	XM_007260586.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	centromere protein J isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035915.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 3, 2017 this sequence version replaced XM_007260586.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCTC CAGCTGGACC ACAGCCATCA GAGTCTCGCT TCCTGTCCCG CTGGATGCCC 
AGCAGCTCTC GGGCCGGGGT CTTCCTGAGT GGCTCTCCAG GAGAGGTCAG CTCTGTCCAG 
AGCAGCTGCT CTTCTCTGGC TCAGGAGATC TCTATGGAAG ACTCTTTCTG CTCCCAGTTC 
GCCCCTCTGC CTGTGTCCTG CAGCAGCAGC CTGGATTCAT TGGTGCCAAA GGTGTCCGAA 
ATAGACCCGC AGAGAAAAGC AACAAGCAGC AACCCAGACA GTGTCCAGGA CATTTTAACA 
AGAGTACAGG AACTTCCATT GCAGGCGAGG CTTGAGCAGC TAAAGCAGTT ACAGCAGCGC 
ATGCAGGAGC AGCTAAAAGC CCATCAGTTA GAGCAGCTGC AAAAACTGCA CAATGAACAG 
CAGGGCATTG TGGGGATGGT ACAGACAGGC GCAGGATACA CCCAAGAAAA ACAGCAGTAC 
AGCAGAAGCA CATCATCTAC ACTTTCCACT GCACAACACA GTGCTTTGCC TACACACCAG 
GAAGAAGTGC TGAGTGAACT TGAGTCAAGT GTGTGGAATC ATGAATCGAG TGTGGTGCAG 
TCGAGCTTTG GGCAAGATGG TGATGGAGAT GAAGATGATA TGATCGAAGG TTCACTGCAG 
TCTGTAATTG AGAATCCAAG CATGTGCAGA CAAGATAGGG AATCAGAGCC TCAAGACAGG 
CCACTCAAAT CAGCTACCAG TGGGAAGACA TTTGAAGAGA TGTTGGAAGA ACAGCTGAAG 
TTGGAGAATG AGAAGATAAA CAAGAACGCT CCTGCTGAAC CAGCAAAGGT CAAGAGGCCG 
TTTCTGAGAC GTGGGGAAGG TCTGGCCAGG TTTAAAAGAG ATAAAGCCTC CGTAACTTTC 
AAAGAAGGAA CAGCTCGGCC AAGCACCAAA CCCACTTCTT GCCCGAATCC TGTGAAGTCT 
CAGGGCCAAG CACTTGCATC CCGTCACCAA AACTCAGAGC CAAAAAGACC TTCGCCTAAA 
CCTACACCAA CTAATACTGT AATCCAACGC AAGACTGCAG TTCTGAACAA AGAGAACTTG 
CCTCAGAGCA AGTCTTTACT ACCAGCAAGC AAAACTGTTG TCAAAGCTCA TATCCTTGGA 
TCCCACCAGG GTCAGAACAT GGGCTCTGGA GCTGTCCAGC AACTTGGCAG GAGTGCTCGG 
AGGCCTGATC CTCCGCAGAA GATTGTCTGT GATGCTGTGG AGGATGTAAA TAAGGGGCCA 
TCACCTGGGC ATCTCCGGGA TCCTAGTACA TGTGTGGCGG ATAACTCCTT TGAAGTGTGG 
CTTGCAGAGC GCGGGGATCG TTGGGAAAAG GATAATCAAC GTGAGTGTGT GGAGCTTGGT 
GAGTTTGAGC TGCTGGAGAG AGCAGCGGAT GAACTATCAT TCTCAAGCAA CTCTTCCTTT 
ATTTGTACTC TTCTTCAGGG AGATCGTAGA CGTCTGTCCT CAACGCCAGT CAAATCACCT 
CCAAAACCTA AGTTGCCTGG TCATATTCAA GCTGACTCTA GAAACCCAGT GCAGCCGAGC 
ATTAGCAGTG TCCAGCAGGG TGTACCAGAC ATGCCTGTAG ACAGTGTTGC AGTAAACATT 
AAGAAGAGTG TTTTGGTCAG CCAGTATTCG GAGGAGGAAG ATGAGGGTAG TAAAATAGAT 
GATGATGAAG ATTCTGATGC TTCATTGTGT AGCACTACAG AGTTCCAACA AACTGCATTT 
GGACCAGGTC AGCCTTTCAG CCACTGCTTT CAAATATCTC AGGTCCCGCC CTACAATAAA 
AGCTCCTACC AGGACAGGGA GGAAGGAGCT GCCAGTCCAC TCGAGGAAGA GGAAAGCAAT 
GGAGATTCTA CTATAGTGGA TGCCAAGGAT CACATTGACT TTGATGACGA TGACACTTGG 
AATGAGCCTG AGGACTTGAC CTGCATTTCC GCTAAGATGG ACGATCATGC AGGCACGACA 
CTGAAGAGGA AGGTTGCCTT CTCAAAAGGA CCAGAGCTTG ACCCTTCTGC TGACAGCTCA 
CTGGGCCGTG AACCAGAGCC TCTGCCCACA TGCCAGTTGG TGGCAAAGCT GTTCCCAGCC 
CTCAAGCCGA AGCCCTGCCC TCCAGCTCCT CCAGTTGCAA AGAATCCAGA AGCAACATGC 
GACGAACAAG GAGCAGCCCA GTCCCGCTTA CTTCGTGAGC GTTTGGTGGA GCTGGAAACA 
GAGATTGAGA GGTTCAAGAC AGAAAATGCG GTGTTGAGCA GACTCAGACA GGAAAACGAG 
GATGTCAGAG AGAACCTGAG GAAAGAGACA GCCCAGTTTC AGCAGAAGAT GGCAGATGAA 
CTTGCCAAAT GGGAAGAGTT CAAGCGCGAG GAAAGCCGGA AGCTGCAGCG GGAAAAGAAG 
CTGTTTGAGA AGCATGCGGC AGCTGCCAGG GCGAGACCTG ACAAACAGGA GCGAGATGAG 
ATGCAGTCTC TAAAGCAGCA ACTGAACTCT CTGCAAGAGG ATTTAAGAAA ACGAGAAGCT 
CGCTGGTCCA GCACCCATCA ACGCCTCCGG CAGCAGGTAG ACACCCTGAG TGCTGAGAAC 
ACGACTCTCC GAGGCCAGGT CCGCATGCTG GAGAAACTCC GCCTGACTGC TTGGAAAAAT 
GCTGAGAGTG AGCGTGAAAA AGAGAAAGCG AGGGGAAGGT CTAATGCACC TGGTGGTGGT 
GGTGGCAGCA CCACCAGTGT CACAGCTCAG AGAAGGGCCA AATCCAAGAG TCCTTCCCAC 
AGTGTAAAGA CCAGCAGCAC GAGCAGCAAA AGGGAAAGCC CAGAGCTTCA GAATCCTCAT 
AAAATTCAAG CTTGTAAATC AAGGTTTAAG CCTACTCTGA GTGAAAAGGA AGCCTGCCCG 
GTGGCTGATC CATACCACAT ATCTGATGGA CAACCTGAGC CTGAAGGGAG GAGCCATGAT 
TCTCTTCCAA TCAATGAATC CTCTTTAGAG GTGGAAAGTC CTAGAGCTGT TACAGAGCAG 
CTGGATATGG AACAAGAAGA AATCACGCAC ACTGATGGCA AGATTGAAAA GCTTCTTCCA 
GATGGAGGGA GACTTATAGT GTTCCCTAAC GGCACAAGGA AAGAGCTGTC TGCAGACGGG 
CTGTCGATCA AAGTCACTTT TTTTAATGGA GACATCAAAC ACATCATGCC TGATCAAAGA 
GTGATTTATT ACTATGCAGG AGCTCAGACC ACACATACGA CCTACCCTGA TGGCATAGAA 
GTTCTCCAGT TCCCTAATAA TCAGATAGAA AAACACTTCC CTGATGGCCA TAAGGAGATC 
ACCTTCCCTG ATCAGACAGT TAAGAATATC TACCCTGATG GGAGAGAGGA GAGCGTACTG 
GCAGACGGAA CAATAATACA GCAAAACCCG GATGGCAGTA AGAAGATCCA GTTTAACAAC 
GGTCAGCGGG AACTTCACAC TGCTGAATTC AAACGCCGAG AATATCCTGA CGGTACGGTC 
AAAACAGTTT ATTCAGATGG CCGCCAGGAA ACACGATATC CCAGTGGCCG CGTTCGACTC 
AAAGACCCTC AGGGACGTGT TGTTATGGAC GCCAAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007260648.2
CDS	163..3744
Translation	MSSPAGPQPSESRFLSRWMPSSSRAGVFLSGSPGEVSSVQSSCSSLAQEISMEDSFCSQFAPLPVSCSSSLDSLVPKVSEIDPQRKATSSNPDSVQDILTRVQELPLQARLEQLKQLQQRMQEQLKAHQLEQLQKLHNEQQGIVGMVQTGAGYTQEKQQYSRSTSSTLSTAQHSALPTHQEEVLSELESSVWNHESSVVQSSFGQDGDGDEDDMIEGSLQSVIENPSMCRQDRESEPQDRPLKSATSGKTFEEMLEEQLKLENEKINKNAPAEPAKVKRPFLRRGEGLARFKRDKASVTFKEGTARPSTKPTSCPNPVKSQGQALASRHQNSEPKRPSPKPTPTNTVIQRKTAVLNKENLPQSKSLLPASKTVVKAHILGSHQGQNMGSGAVQQLGRSARRPDPPQKIVCDAVEDVNKGPSPGHLRDPSTCVADNSFEVWLAERGDRWEKDNQRECVELGEFELLERAADELSFSSNSSFICTLLQGDRRRLSSTPVKSPPKPKLPGHIQADSRNPVQPSISSVQQGVPDMPVDSVAVNIKKSVLVSQYSEEEDEGSKIDDDEDSDASLCSTTEFQQTAFGPGQPFSHCFQISQVPPYNKSSYQDREEGAASPLEEEESNGDSTIVDAKDHIDFDDDDTWNEPEDLTCISAKMDDHAGTTLKRKVAFSKGPELDPSADSSLGREPEPLPTCQLVAKLFPALKPKPCPPAPPVAKNPEATCDEQGAAQSRLLRERLVELETEIERFKTENAVLSRLRQENEDVRENLRKETAQFQQKMADELAKWEEFKREESRKLQREKKLFEKHAAAARARPDKQERDEMQSLKQQLNSLQEDLRKREARWSSTHQRLRQQVDTLSAENTTLRGQVRMLEKLRLTAWKNAESEREKEKARGRSNAPGGGGGSTTSVTAQRRAKSKSPSHSVKTSSTSSKRESPELQNPHKIQACKSRFKPTLSEKEACPVADPYHISDGQPEPEGRSHDSLPINESSLEVESPRAVTEQLDMEQEEITHTDGKIEKLLPDGGRLIVFPNGTRKELSADGLSIKVTFFNGDIKHIMPDQRVIYYYAGAQTTHTTYPDGIEVLQFPNNQIEKHFPDGHKEITFPDQTVKNIYPDGREESVLADGTIIQQNPDGSKKIQFNNGQRELHTAEFKRREYPDGTVKTVYSDGRQETRYPSGRVRLKDPQGRVVMDAKP

Target ORF information:

RefSeq Version	XM_007260586.3
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus centromere protein J (cenpj), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007260586.3

ORF Insert Sequence:

ATGTCATCTC CAGCTGGACC ACAGCCATCA GAGTCTCGCT TCCTGTCCCG CTGGATGCCC 
AGCAGCTCTC GGGCCGGGGT CTTCCTGAGT GGCTCTCCAG GAGAGGTCAG CTCTGTCCAG 
AGCAGCTGCT CTTCTCTGGC TCAGGAGATC TCTATGGAAG ACTCTTTCTG CTCCCAGTTC 
GCCCCTCTGC CTGTGTCCTG CAGCAGCAGC CTGGATTCAT TGGTGCCAAA GGTGTCCGAA 
ATAGACCCGC AGAGAAAAGC AACAAGCAGC AACCCAGACA GTGTCCAGGA CATTTTAACA 
AGAGTACAGG AACTTCCATT GCAGGCGAGG CTTGAGCAGC TAAAGCAGTT ACAGCAGCGC 
ATGCAGGAGC AGCTAAAAGC CCATCAGTTA GAGCAGCTGC AAAAACTGCA CAATGAACAG 
CAGGGCATTG TGGGGATGGT ACAGACAGGC GCAGGATACA CCCAAGAAAA ACAGCAGTAC 
AGCAGAAGCA CATCATCTAC ACTTTCCACT GCACAACACA GTGCTTTGCC TACACACCAG 
GAAGAAGTGC TGAGTGAACT TGAGTCAAGT GTGTGGAATC ATGAATCGAG TGTGGTGCAG 
TCGAGCTTTG GGCAAGATGG TGATGGAGAT GAAGATGATA TGATCGAAGG TTCACTGCAG 
TCTGTAATTG AGAATCCAAG CATGTGCAGA CAAGATAGGG AATCAGAGCC TCAAGACAGG 
CCACTCAAAT CAGCTACCAG TGGGAAGACA TTTGAAGAGA TGTTGGAAGA ACAGCTGAAG 
TTGGAGAATG AGAAGATAAA CAAGAACGCT CCTGCTGAAC CAGCAAAGGT CAAGAGGCCG 
TTTCTGAGAC GTGGGGAAGG TCTGGCCAGG TTTAAAAGAG ATAAAGCCTC CGTAACTTTC 
AAAGAAGGAA CAGCTCGGCC AAGCACCAAA CCCACTTCTT GCCCGAATCC TGTGAAGTCT 
CAGGGCCAAG CACTTGCATC CCGTCACCAA AACTCAGAGC CAAAAAGACC TTCGCCTAAA 
CCTACACCAA CTAATACTGT AATCCAACGC AAGACTGCAG TTCTGAACAA AGAGAACTTG 
CCTCAGAGCA AGTCTTTACT ACCAGCAAGC AAAACTGTTG TCAAAGCTCA TATCCTTGGA 
TCCCACCAGG GTCAGAACAT GGGCTCTGGA GCTGTCCAGC AACTTGGCAG GAGTGCTCGG 
AGGCCTGATC CTCCGCAGAA GATTGTCTGT GATGCTGTGG AGGATGTAAA TAAGGGGCCA 
TCACCTGGGC ATCTCCGGGA TCCTAGTACA TGTGTGGCGG ATAACTCCTT TGAAGTGTGG 
CTTGCAGAGC GCGGGGATCG TTGGGAAAAG GATAATCAAC GTGAGTGTGT GGAGCTTGGT 
GAGTTTGAGC TGCTGGAGAG AGCAGCGGAT GAACTATCAT TCTCAAGCAA CTCTTCCTTT 
ATTTGTACTC TTCTTCAGGG AGATCGTAGA CGTCTGTCCT CAACGCCAGT CAAATCACCT 
CCAAAACCTA AGTTGCCTGG TCATATTCAA GCTGACTCTA GAAACCCAGT GCAGCCGAGC 
ATTAGCAGTG TCCAGCAGGG TGTACCAGAC ATGCCTGTAG ACAGTGTTGC AGTAAACATT 
AAGAAGAGTG TTTTGGTCAG CCAGTATTCG GAGGAGGAAG ATGAGGGTAG TAAAATAGAT 
GATGATGAAG ATTCTGATGC TTCATTGTGT AGCACTACAG AGTTCCAACA AACTGCATTT 
GGACCAGGTC AGCCTTTCAG CCACTGCTTT CAAATATCTC AGGTCCCGCC CTACAATAAA 
AGCTCCTACC AGGACAGGGA GGAAGGAGCT GCCAGTCCAC TCGAGGAAGA GGAAAGCAAT 
GGAGATTCTA CTATAGTGGA TGCCAAGGAT CACATTGACT TTGATGACGA TGACACTTGG 
AATGAGCCTG AGGACTTGAC CTGCATTTCC GCTAAGATGG ACGATCATGC AGGCACGACA 
CTGAAGAGGA AGGTTGCCTT CTCAAAAGGA CCAGAGCTTG ACCCTTCTGC TGACAGCTCA 
CTGGGCCGTG AACCAGAGCC TCTGCCCACA TGCCAGTTGG TGGCAAAGCT GTTCCCAGCC 
CTCAAGCCGA AGCCCTGCCC TCCAGCTCCT CCAGTTGCAA AGAATCCAGA AGCAACATGC 
GACGAACAAG GAGCAGCCCA GTCCCGCTTA CTTCGTGAGC GTTTGGTGGA GCTGGAAACA 
GAGATTGAGA GGTTCAAGAC AGAAAATGCG GTGTTGAGCA GACTCAGACA GGAAAACGAG 
GATGTCAGAG AGAACCTGAG GAAAGAGACA GCCCAGTTTC AGCAGAAGAT GGCAGATGAA 
CTTGCCAAAT GGGAAGAGTT CAAGCGCGAG GAAAGCCGGA AGCTGCAGCG GGAAAAGAAG 
CTGTTTGAGA AGCATGCGGC AGCTGCCAGG GCGAGACCTG ACAAACAGGA GCGAGATGAG 
ATGCAGTCTC TAAAGCAGCA ACTGAACTCT CTGCAAGAGG ATTTAAGAAA ACGAGAAGCT 
CGCTGGTCCA GCACCCATCA ACGCCTCCGG CAGCAGGTAG ACACCCTGAG TGCTGAGAAC 
ACGACTCTCC GAGGCCAGGT CCGCATGCTG GAGAAACTCC GCCTGACTGC TTGGAAAAAT 
GCTGAGAGTG AGCGTGAAAA AGAGAAAGCG AGGGGAAGGT CTAATGCACC TGGTGGTGGT 
GGTGGCAGCA CCACCAGTGT CACAGCTCAG AGAAGGGCCA AATCCAAGAG TCCTTCCCAC 
AGTGTAAAGA CCAGCAGCAC GAGCAGCAAA AGGGAAAGCC CAGAGCTTCA GAATCCTCAT 
AAAATTCAAG CTTGTAAATC AAGGTTTAAG CCTACTCTGA GTGAAAAGGA AGCCTGCCCG 
GTGGCTGATC CATACCACAT ATCTGATGGA CAACCTGAGC CTGAAGGGAG GAGCCATGAT 
TCTCTTCCAA TCAATGAATC CTCTTTAGAG GTGGAAAGTC CTAGAGCTGT TACAGAGCAG 
CTGGATATGG AACAAGAAGA AATCACGCAC ACTGATGGCA AGATTGAAAA GCTTCTTCCA 
GATGGAGGGA GACTTATAGT GTTCCCTAAC GGCACAAGGA AAGAGCTGTC TGCAGACGGG 
CTGTCGATCA AAGTCACTTT TTTTAATGGA GACATCAAAC ACATCATGCC TGATCAAAGA 
GTGATTTATT ACTATGCAGG AGCTCAGACC ACACATACGA CCTACCCTGA TGGCATAGAA 
GTTCTCCAGT TCCCTAATAA TCAGATAGAA AAACACTTCC CTGATGGCCA TAAGGAGATC 
ACCTTCCCTG ATCAGACAGT TAAGAATATC TACCCTGATG GGAGAGAGGA GAGCGTACTG 
GCAGACGGAA CAATAATACA GCAAAACCCG GATGGCAGTA AGAAGATCCA GTTTAACAAC 
GGTCAGCGGG AACTTCACAC TGCTGAATTC AAACGCCGAG AATATCCTGA CGGTACGGTC 
AAAACAGTTT ATTCAGATGG CCGCCAGGAA ACACGATATC CCAGTGGCCG CGTTCGACTC 
AAAGACCCTC AGGGACGTGT TGTTATGGAC GCCAAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cenpj ( XM_007260585.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007260647.1 Astyanax mexicanus centromere protein J (cenpj), transcript variant X1, mRNA.

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CloneID	OAs42146
Clone ID Related Accession (Same CDS sequence)	XM_007260585.2
Accession Version	XM_007260585.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	centromere protein J isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749487.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007260585.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCTC CAGCTGGACC ACAGCCATCC GAGTCTCGCT TCCTGTCCCG CTGGATGCCC 
AGCAGCTCTC GGGCCGGGGT CTTCCTGAGT GGCTCTCCAG GAGAGGTCAG CTCTGTCCAG 
AGCAGCTGCT CTTCTCTGGC TCAGGAGATA TCTATGGAAG ACTCTTTCTG CTCCCAGTTC 
GCCCCTCTGC CTGTGTCCTG CAGCAGCAGC CTGGATTCAT TGGTGCCAAA GGTGTCCGAA 
ATAGACCCGC AGAGAAAAGC AACAAGCAGC AACCCAGACA GTGTCCAGGA CATTTTAACA 
AGAGTACAGG AACTTCCACT GCAGGCGAGG CTTGAGCAGC TAAAGCAGTT ACAGCAGCGC 
ATGCAGGAGC AGCTAAAAGC CCATCAGTTA GAGCAGCTGC AAAAACTGCA CAATGAACAG 
CAGGGCATTG TGGGGATGGT ACAGACAGGC GCAGCAGGAT ACACCCAAGA AAAACAGCAG 
TACAGCAGAA GCACATCATC TACACTTTCC ACTGCACAAC ACAGTGCTTT GCCTACACAC 
CAGGAAGAAG TGCTGAGTGA ACTTGAGTCA AGTGTGTGGA ATCATGAATC GAGTGTGGTG 
CAGTCGAGCT TTGGGCAAGA TGGTGATGGA GATGAAGATG ATATGATCAA AGGTTCACTG 
CAGTCTGTAA TTGAGAATCC AAGCATGTGC AGACAAGATA GGGAATCAGA GCCTCAAGAC 
AGGCCACTCA AATCAGCTAC CAGTGGGAAG ACATTTGAAG AGATGTTGGA AGAACAGCTG 
AAGTTGGAGA ATGAGAAGAT AAACAAGAAC GCTCCTGCTG AACCAGCAAA GGTCAAGAGG 
CCGTTTCTGA GACGTGGGGA AGGTCTGGCC AGGTTTAAAA GAGATAAAGC CTCCGTAACT 
TTCAAAGAAG GAACAGCTCG GCCAAGCACC AAACCCACTT CTTGCCCGAA TCCTGTGAAG 
TCTCAGGGCC AAGCACTTGC ATCCCGTCAC CAAAACTCAG AGCCAAAAAG ACCTTCGCCT 
AAACCTACAC CAACTAATAC TGTAATCCAA CGCAAGACTG CAGTTCTGAA CAAAGAGAAC 
TTGCCTCAGA GCAAGTCTTT ACTACCAGCA AGCAAAACTG TTGTCAAAGC TCATATCCTT 
GGATCCCACC AGGGTCAGAA CATGGGCTCT GGAGCTGTCC AGCAACTTGG CAGGAGTGCT 
CGGAGGCCTG ATCCTCCGCA GAAGATTGTC TGTGATGCTG TGGAGGATGT AAATAAGGGG 
CCATCACCTG GGCATCTCCG GGATCCTAGT ACATGTGTGG CGGAGAACTC CTTTGAAGTG 
TGGCTTGCAG AGCGCGGGGA TCGTTGGGAA AAGGATAATC AACGTGAGTG TGTGGAGCTT 
GGTGAGTTTG AGCTGCTGGA GAGAGCAGCG GATGAACTAT CATTCTCAAG CAACTCTTCC 
TTTATTTGTA CTCTTCTTCA GGGAGATCGT AGACGTCTGT CCTCAACGCC AGTCAAATCA 
CCTCCAAAAC CTAAGTTGCC TGGTCATATT CAAGCTGACT CTAGAAACCC AGTGCAGCCG 
AGCATTAGCA GTGTCCAGCA GGGTGTACCA GACATGCCTG TAGCCAGTGT TGCAGTAAAC 
ATTAAGAAGA GTGTGGTGGT CAGCCAGTAT TCGGAGGAGG AAGATGAGGG TAGTAAAATA 
GATGATGATG AAGATTCTGA TGCTTCATTG TGTAGCACTA CAGAGTTCCA ACAAACTGCA 
TTTGGACCAG GTCAGCCTTT CAGCCACTGC TTTCAAATAT CTCAGGTCCC GCCCTACAAT 
AAAAGCTCCT ACCAGGACAG GGAGGAAGGA GCTGCCAGTC CACTCGAGGA AGAGGAAAGC 
AATGGAGATT CTACTATAGT GGATGCCAAG GATCACATTG ACTTTGATGA CGATGACACT 
TGGAATGAGC CTGAGGACTT GACCTGCATT TCCGCTAAGA TGGACGATCA TGCAGGCACG 
ACACTGAAGA GGAAGGTTGC CTTCTCAAAA GGACCAGAGC TTGACCCTTC TGCTGACAGC 
TCACTGGGCC GTGAACCAGA GCCTCTGCCC ACATGCCAGT TGGTAGCAAA GCTGTTCCCA 
GCCCTCAAGC CGAAGCCCTG CCCTCCAGCT CCTCCAGTTG CAAAGAATCC AGAAGCAACA 
TGCGACGAAC AAGGAGCAGC CCAGTCCCGC TTACTTCGTG AGCGTTTGGT GGAGCTGGAA 
ACAGAGATTG AGAGGTTCAA GACAGAAAAT GCGGTGTTGA GCAGACTCAG ACAGGAAAAC 
GAGGATGTCA GAGAGAACCT GAGGAAAGAG ACAGCCCAGT TTCAGCAGAA GATTGCAGAT 
GAACTTGCCA AATGGGAAGA GTTCAAGCGC GAGGAAAGCC GGAAGCTGCA GCGGGAAAAG 
AAGCTGTTTG AGAAGCATGC GGCAGCTGCC AGGGCGAGAC CTGACAAACA GGAGCGAGAT 
GAGATGCAGT CTCTAAAGCA GCAACTGAAC TCTCTGCAAG AGGATTTAAG AAAACGAGAA 
GCTCGCTGGT CCAGCACCCA TCAACGCCTC CGGCAGCAGG TAGACACCCT GAGTGCTGAG 
AACACGACTC TCCGAGGCCA GGTCCGCATG CTGGAGAAAC TCCGCCTGAC TGCTTGGAAA 
AATGCTGAGA GTGAGCGTGA AAAAGAGAAA GAGAGGGGAA GGTCTAATGC ACCTGGTGGT 
GGTGGCGGCA GCACCACCAG TGTCACAGCT CAGAGAAGGG CCAAATCCAA GAGTCCTTCC 
CACAGTGTAA AGACCAGCAG CACGAGCAGC AAAAGGGAAA GCCCAGAGCT TCAGAATCCT 
CATAAAATTC AAGCTTGTAA ATCAAGGTTT AAGCCTACTC TGAGTGAAAA GGAAGCCTGC 
CCGGTGGCTG ATCCATACCA CATATCTGAT GGACAACCTG AGCCTGAAGG GAGGAGCCAT 
GATTCTCTTC CAATCAATGA ATCCTCTTTA GAGGTGGAAA GTCCTAGAGC TGTTACAGAG 
CAGCTGGATA TGGAACAAGA AGAAATCACG CACACTGATG GCAAGATTGA AAAGCTTCTT 
CCAGATGGAG GGAGACTTAT AGTGTTCCCT AACGGCACAA GGAAAGAGCT GTCTGCAGAC 
GGGCTGTCGA TCAAAGTCAC TTTTTTTAAT GGAGACATCA AACACATCAT GCCTGATCAA 
AGAGTGATTT ATTACTATGC AGGAGCTCAG ACCACACATA CGACCTACCC TGATGGCATA 
GAAGTTCTCC AGTTCCCTAA TAATCAGATA GAAAAACACT TCCCTGATGG CCATAAGGAG 
ATCACCTTCC CTGATCAGAC AGTTAAGAAT ATCTACCCTG ATGGGAGAGA GGAGAGCGTA 
CTGGCAGACG GAACAATAAT ACAGCAAAAC CCGGATGGCA GTAAGAAGAT CCAGTTTAAC 
AACGGTCAGC GGGAACTTCA CACTGCTGAA TTCAAACGCC GAGAATATCC TGACGGTACG 
GTCAAAACAG TTTATTCAGA TGGCCGCCAG GAAACACGAT ATCCCAGTGG CCGCGTTCGA 
CTCAAAGACC CTCAGGGACG TGTTGTTATG GACGCCAAGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007260647.1
CDS	136..3720
Translation	MSSPAGPQPSESRFLSRWMPSSSRAGVFLSGSPGEVSSVQSSCSSLAQEISMEDSFCSQFAPLPVSCSSSLDSLVPKVSEIDPQRKATSSNPDSVQDILTRVQELPLQARLEQLKQLQQRMQEQLKAHQLEQLQKLHNEQQGIVGMVQTGAAGYTQEKQQYSRSTSSTLSTAQHSALPTHQEEVLSELESSVWNHESSVVQSSFGQDGDGDEDDMIKGSLQSVIENPSMCRQDRESEPQDRPLKSATSGKTFEEMLEEQLKLENEKINKNAPAEPAKVKRPFLRRGEGLARFKRDKASVTFKEGTARPSTKPTSCPNPVKSQGQALASRHQNSEPKRPSPKPTPTNTVIQRKTAVLNKENLPQSKSLLPASKTVVKAHILGSHQGQNMGSGAVQQLGRSARRPDPPQKIVCDAVEDVNKGPSPGHLRDPSTCVAENSFEVWLAERGDRWEKDNQRECVELGEFELLERAADELSFSSNSSFICTLLQGDRRRLSSTPVKSPPKPKLPGHIQADSRNPVQPSISSVQQGVPDMPVASVAVNIKKSVVVSQYSEEEDEGSKIDDDEDSDASLCSTTEFQQTAFGPGQPFSHCFQISQVPPYNKSSYQDREEGAASPLEEEESNGDSTIVDAKDHIDFDDDDTWNEPEDLTCISAKMDDHAGTTLKRKVAFSKGPELDPSADSSLGREPEPLPTCQLVAKLFPALKPKPCPPAPPVAKNPEATCDEQGAAQSRLLRERLVELETEIERFKTENAVLSRLRQENEDVRENLRKETAQFQQKIADELAKWEEFKREESRKLQREKKLFEKHAAAARARPDKQERDEMQSLKQQLNSLQEDLRKREARWSSTHQRLRQQVDTLSAENTTLRGQVRMLEKLRLTAWKNAESEREKEKERGRSNAPGGGGGSTTSVTAQRRAKSKSPSHSVKTSSTSSKRESPELQNPHKIQACKSRFKPTLSEKEACPVADPYHISDGQPEPEGRSHDSLPINESSLEVESPRAVTEQLDMEQEEITHTDGKIEKLLPDGGRLIVFPNGTRKELSADGLSIKVTFFNGDIKHIMPDQRVIYYYAGAQTTHTTYPDGIEVLQFPNNQIEKHFPDGHKEITFPDQTVKNIYPDGREESVLADGTIIQQNPDGSKKIQFNNGQRELHTAEFKRREYPDGTVKTVYSDGRQETRYPSGRVRLKDPQGRVVMDAKP

Target ORF information:

RefSeq Version	XM_007260585.2
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus centromere protein J (cenpj), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007260585.2

ORF Insert Sequence:

ATGTCATCTC CAGCTGGACC ACAGCCATCC GAGTCTCGCT TCCTGTCCCG CTGGATGCCC 
AGCAGCTCTC GGGCCGGGGT CTTCCTGAGT GGCTCTCCAG GAGAGGTCAG CTCTGTCCAG 
AGCAGCTGCT CTTCTCTGGC TCAGGAGATA TCTATGGAAG ACTCTTTCTG CTCCCAGTTC 
GCCCCTCTGC CTGTGTCCTG CAGCAGCAGC CTGGATTCAT TGGTGCCAAA GGTGTCCGAA 
ATAGACCCGC AGAGAAAAGC AACAAGCAGC AACCCAGACA GTGTCCAGGA CATTTTAACA 
AGAGTACAGG AACTTCCACT GCAGGCGAGG CTTGAGCAGC TAAAGCAGTT ACAGCAGCGC 
ATGCAGGAGC AGCTAAAAGC CCATCAGTTA GAGCAGCTGC AAAAACTGCA CAATGAACAG 
CAGGGCATTG TGGGGATGGT ACAGACAGGC GCAGCAGGAT ACACCCAAGA AAAACAGCAG 
TACAGCAGAA GCACATCATC TACACTTTCC ACTGCACAAC ACAGTGCTTT GCCTACACAC 
CAGGAAGAAG TGCTGAGTGA ACTTGAGTCA AGTGTGTGGA ATCATGAATC GAGTGTGGTG 
CAGTCGAGCT TTGGGCAAGA TGGTGATGGA GATGAAGATG ATATGATCAA AGGTTCACTG 
CAGTCTGTAA TTGAGAATCC AAGCATGTGC AGACAAGATA GGGAATCAGA GCCTCAAGAC 
AGGCCACTCA AATCAGCTAC CAGTGGGAAG ACATTTGAAG AGATGTTGGA AGAACAGCTG 
AAGTTGGAGA ATGAGAAGAT AAACAAGAAC GCTCCTGCTG AACCAGCAAA GGTCAAGAGG 
CCGTTTCTGA GACGTGGGGA AGGTCTGGCC AGGTTTAAAA GAGATAAAGC CTCCGTAACT 
TTCAAAGAAG GAACAGCTCG GCCAAGCACC AAACCCACTT CTTGCCCGAA TCCTGTGAAG 
TCTCAGGGCC AAGCACTTGC ATCCCGTCAC CAAAACTCAG AGCCAAAAAG ACCTTCGCCT 
AAACCTACAC CAACTAATAC TGTAATCCAA CGCAAGACTG CAGTTCTGAA CAAAGAGAAC 
TTGCCTCAGA GCAAGTCTTT ACTACCAGCA AGCAAAACTG TTGTCAAAGC TCATATCCTT 
GGATCCCACC AGGGTCAGAA CATGGGCTCT GGAGCTGTCC AGCAACTTGG CAGGAGTGCT 
CGGAGGCCTG ATCCTCCGCA GAAGATTGTC TGTGATGCTG TGGAGGATGT AAATAAGGGG 
CCATCACCTG GGCATCTCCG GGATCCTAGT ACATGTGTGG CGGAGAACTC CTTTGAAGTG 
TGGCTTGCAG AGCGCGGGGA TCGTTGGGAA AAGGATAATC AACGTGAGTG TGTGGAGCTT 
GGTGAGTTTG AGCTGCTGGA GAGAGCAGCG GATGAACTAT CATTCTCAAG CAACTCTTCC 
TTTATTTGTA CTCTTCTTCA GGGAGATCGT AGACGTCTGT CCTCAACGCC AGTCAAATCA 
CCTCCAAAAC CTAAGTTGCC TGGTCATATT CAAGCTGACT CTAGAAACCC AGTGCAGCCG 
AGCATTAGCA GTGTCCAGCA GGGTGTACCA GACATGCCTG TAGCCAGTGT TGCAGTAAAC 
ATTAAGAAGA GTGTGGTGGT CAGCCAGTAT TCGGAGGAGG AAGATGAGGG TAGTAAAATA 
GATGATGATG AAGATTCTGA TGCTTCATTG TGTAGCACTA CAGAGTTCCA ACAAACTGCA 
TTTGGACCAG GTCAGCCTTT CAGCCACTGC TTTCAAATAT CTCAGGTCCC GCCCTACAAT 
AAAAGCTCCT ACCAGGACAG GGAGGAAGGA GCTGCCAGTC CACTCGAGGA AGAGGAAAGC 
AATGGAGATT CTACTATAGT GGATGCCAAG GATCACATTG ACTTTGATGA CGATGACACT 
TGGAATGAGC CTGAGGACTT GACCTGCATT TCCGCTAAGA TGGACGATCA TGCAGGCACG 
ACACTGAAGA GGAAGGTTGC CTTCTCAAAA GGACCAGAGC TTGACCCTTC TGCTGACAGC 
TCACTGGGCC GTGAACCAGA GCCTCTGCCC ACATGCCAGT TGGTAGCAAA GCTGTTCCCA 
GCCCTCAAGC CGAAGCCCTG CCCTCCAGCT CCTCCAGTTG CAAAGAATCC AGAAGCAACA 
TGCGACGAAC AAGGAGCAGC CCAGTCCCGC TTACTTCGTG AGCGTTTGGT GGAGCTGGAA 
ACAGAGATTG AGAGGTTCAA GACAGAAAAT GCGGTGTTGA GCAGACTCAG ACAGGAAAAC 
GAGGATGTCA GAGAGAACCT GAGGAAAGAG ACAGCCCAGT TTCAGCAGAA GATTGCAGAT 
GAACTTGCCA AATGGGAAGA GTTCAAGCGC GAGGAAAGCC GGAAGCTGCA GCGGGAAAAG 
AAGCTGTTTG AGAAGCATGC GGCAGCTGCC AGGGCGAGAC CTGACAAACA GGAGCGAGAT 
GAGATGCAGT CTCTAAAGCA GCAACTGAAC TCTCTGCAAG AGGATTTAAG AAAACGAGAA 
GCTCGCTGGT CCAGCACCCA TCAACGCCTC CGGCAGCAGG TAGACACCCT GAGTGCTGAG 
AACACGACTC TCCGAGGCCA GGTCCGCATG CTGGAGAAAC TCCGCCTGAC TGCTTGGAAA 
AATGCTGAGA GTGAGCGTGA AAAAGAGAAA GAGAGGGGAA GGTCTAATGC ACCTGGTGGT 
GGTGGCGGCA GCACCACCAG TGTCACAGCT CAGAGAAGGG CCAAATCCAA GAGTCCTTCC 
CACAGTGTAA AGACCAGCAG CACGAGCAGC AAAAGGGAAA GCCCAGAGCT TCAGAATCCT 
CATAAAATTC AAGCTTGTAA ATCAAGGTTT AAGCCTACTC TGAGTGAAAA GGAAGCCTGC 
CCGGTGGCTG ATCCATACCA CATATCTGAT GGACAACCTG AGCCTGAAGG GAGGAGCCAT 
GATTCTCTTC CAATCAATGA ATCCTCTTTA GAGGTGGAAA GTCCTAGAGC TGTTACAGAG 
CAGCTGGATA TGGAACAAGA AGAAATCACG CACACTGATG GCAAGATTGA AAAGCTTCTT 
CCAGATGGAG GGAGACTTAT AGTGTTCCCT AACGGCACAA GGAAAGAGCT GTCTGCAGAC 
GGGCTGTCGA TCAAAGTCAC TTTTTTTAAT GGAGACATCA AACACATCAT GCCTGATCAA 
AGAGTGATTT ATTACTATGC AGGAGCTCAG ACCACACATA CGACCTACCC TGATGGCATA 
GAAGTTCTCC AGTTCCCTAA TAATCAGATA GAAAAACACT TCCCTGATGG CCATAAGGAG 
ATCACCTTCC CTGATCAGAC AGTTAAGAAT ATCTACCCTG ATGGGAGAGA GGAGAGCGTA 
CTGGCAGACG GAACAATAAT ACAGCAAAAC CCGGATGGCA GTAAGAAGAT CCAGTTTAAC 
AACGGTCAGC GGGAACTTCA CACTGCTGAA TTCAAACGCC GAGAATATCC TGACGGTACG 
GTCAAAACAG TTTATTCAGA TGGCCGCCAG GAAACACGAT ATCCCAGTGG CCGCGTTCGA 
CTCAAAGACC CTCAGGGACG TGTTGTTATG GACGCCAAGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cenpj ( XM_007260586.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007260648.1 Astyanax mexicanus centromere protein J (cenpj), transcript variant X2, mRNA.

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CloneID	OAs42147
Clone ID Related Accession (Same CDS sequence)	XM_007260586.2
Accession Version	XM_007260586.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	centromere protein J isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749487.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007260586.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCTC CAGCTGGACC ACAGCCATCC GAGTCTCGCT TCCTGTCCCG CTGGATGCCC 
AGCAGCTCTC GGGCCGGGGT CTTCCTGAGT GGCTCTCCAG GAGAGGTCAG CTCTGTCCAG 
AGCAGCTGCT CTTCTCTGGC TCAGGAGATA TCTATGGAAG ACTCTTTCTG CTCCCAGTTC 
GCCCCTCTGC CTGTGTCCTG CAGCAGCAGC CTGGATTCAT TGGTGCCAAA GGTGTCCGAA 
ATAGACCCGC AGAGAAAAGC AACAAGCAGC AACCCAGACA GTGTCCAGGA CATTTTAACA 
AGAGTACAGG AACTTCCACT GCAGGCGAGG CTTGAGCAGC TAAAGCAGTT ACAGCAGCGC 
ATGCAGGAGC AGCTAAAAGC CCATCAGTTA GAGCAGCTGC AAAAACTGCA CAATGAACAG 
CAGGGCATTG TGGGGATGGT ACAGACAGGC GCAGGATACA CCCAAGAAAA ACAGCAGTAC 
AGCAGAAGCA CATCATCTAC ACTTTCCACT GCACAACACA GTGCTTTGCC TACACACCAG 
GAAGAAGTGC TGAGTGAACT TGAGTCAAGT GTGTGGAATC ATGAATCGAG TGTGGTGCAG 
TCGAGCTTTG GGCAAGATGG TGATGGAGAT GAAGATGATA TGATCAAAGG TTCACTGCAG 
TCTGTAATTG AGAATCCAAG CATGTGCAGA CAAGATAGGG AATCAGAGCC TCAAGACAGG 
CCACTCAAAT CAGCTACCAG TGGGAAGACA TTTGAAGAGA TGTTGGAAGA ACAGCTGAAG 
TTGGAGAATG AGAAGATAAA CAAGAACGCT CCTGCTGAAC CAGCAAAGGT CAAGAGGCCG 
TTTCTGAGAC GTGGGGAAGG TCTGGCCAGG TTTAAAAGAG ATAAAGCCTC CGTAACTTTC 
AAAGAAGGAA CAGCTCGGCC AAGCACCAAA CCCACTTCTT GCCCGAATCC TGTGAAGTCT 
CAGGGCCAAG CACTTGCATC CCGTCACCAA AACTCAGAGC CAAAAAGACC TTCGCCTAAA 
CCTACACCAA CTAATACTGT AATCCAACGC AAGACTGCAG TTCTGAACAA AGAGAACTTG 
CCTCAGAGCA AGTCTTTACT ACCAGCAAGC AAAACTGTTG TCAAAGCTCA TATCCTTGGA 
TCCCACCAGG GTCAGAACAT GGGCTCTGGA GCTGTCCAGC AACTTGGCAG GAGTGCTCGG 
AGGCCTGATC CTCCGCAGAA GATTGTCTGT GATGCTGTGG AGGATGTAAA TAAGGGGCCA 
TCACCTGGGC ATCTCCGGGA TCCTAGTACA TGTGTGGCGG AGAACTCCTT TGAAGTGTGG 
CTTGCAGAGC GCGGGGATCG TTGGGAAAAG GATAATCAAC GTGAGTGTGT GGAGCTTGGT 
GAGTTTGAGC TGCTGGAGAG AGCAGCGGAT GAACTATCAT TCTCAAGCAA CTCTTCCTTT 
ATTTGTACTC TTCTTCAGGG AGATCGTAGA CGTCTGTCCT CAACGCCAGT CAAATCACCT 
CCAAAACCTA AGTTGCCTGG TCATATTCAA GCTGACTCTA GAAACCCAGT GCAGCCGAGC 
ATTAGCAGTG TCCAGCAGGG TGTACCAGAC ATGCCTGTAG CCAGTGTTGC AGTAAACATT 
AAGAAGAGTG TGGTGGTCAG CCAGTATTCG GAGGAGGAAG ATGAGGGTAG TAAAATAGAT 
GATGATGAAG ATTCTGATGC TTCATTGTGT AGCACTACAG AGTTCCAACA AACTGCATTT 
GGACCAGGTC AGCCTTTCAG CCACTGCTTT CAAATATCTC AGGTCCCGCC CTACAATAAA 
AGCTCCTACC AGGACAGGGA GGAAGGAGCT GCCAGTCCAC TCGAGGAAGA GGAAAGCAAT 
GGAGATTCTA CTATAGTGGA TGCCAAGGAT CACATTGACT TTGATGACGA TGACACTTGG 
AATGAGCCTG AGGACTTGAC CTGCATTTCC GCTAAGATGG ACGATCATGC AGGCACGACA 
CTGAAGAGGA AGGTTGCCTT CTCAAAAGGA CCAGAGCTTG ACCCTTCTGC TGACAGCTCA 
CTGGGCCGTG AACCAGAGCC TCTGCCCACA TGCCAGTTGG TAGCAAAGCT GTTCCCAGCC 
CTCAAGCCGA AGCCCTGCCC TCCAGCTCCT CCAGTTGCAA AGAATCCAGA AGCAACATGC 
GACGAACAAG GAGCAGCCCA GTCCCGCTTA CTTCGTGAGC GTTTGGTGGA GCTGGAAACA 
GAGATTGAGA GGTTCAAGAC AGAAAATGCG GTGTTGAGCA GACTCAGACA GGAAAACGAG 
GATGTCAGAG AGAACCTGAG GAAAGAGACA GCCCAGTTTC AGCAGAAGAT TGCAGATGAA 
CTTGCCAAAT GGGAAGAGTT CAAGCGCGAG GAAAGCCGGA AGCTGCAGCG GGAAAAGAAG 
CTGTTTGAGA AGCATGCGGC AGCTGCCAGG GCGAGACCTG ACAAACAGGA GCGAGATGAG 
ATGCAGTCTC TAAAGCAGCA ACTGAACTCT CTGCAAGAGG ATTTAAGAAA ACGAGAAGCT 
CGCTGGTCCA GCACCCATCA ACGCCTCCGG CAGCAGGTAG ACACCCTGAG TGCTGAGAAC 
ACGACTCTCC GAGGCCAGGT CCGCATGCTG GAGAAACTCC GCCTGACTGC TTGGAAAAAT 
GCTGAGAGTG AGCGTGAAAA AGAGAAAGAG AGGGGAAGGT CTAATGCACC TGGTGGTGGT 
GGCGGCAGCA CCACCAGTGT CACAGCTCAG AGAAGGGCCA AATCCAAGAG TCCTTCCCAC 
AGTGTAAAGA CCAGCAGCAC GAGCAGCAAA AGGGAAAGCC CAGAGCTTCA GAATCCTCAT 
AAAATTCAAG CTTGTAAATC AAGGTTTAAG CCTACTCTGA GTGAAAAGGA AGCCTGCCCG 
GTGGCTGATC CATACCACAT ATCTGATGGA CAACCTGAGC CTGAAGGGAG GAGCCATGAT 
TCTCTTCCAA TCAATGAATC CTCTTTAGAG GTGGAAAGTC CTAGAGCTGT TACAGAGCAG 
CTGGATATGG AACAAGAAGA AATCACGCAC ACTGATGGCA AGATTGAAAA GCTTCTTCCA 
GATGGAGGGA GACTTATAGT GTTCCCTAAC GGCACAAGGA AAGAGCTGTC TGCAGACGGG 
CTGTCGATCA AAGTCACTTT TTTTAATGGA GACATCAAAC ACATCATGCC TGATCAAAGA 
GTGATTTATT ACTATGCAGG AGCTCAGACC ACACATACGA CCTACCCTGA TGGCATAGAA 
GTTCTCCAGT TCCCTAATAA TCAGATAGAA AAACACTTCC CTGATGGCCA TAAGGAGATC 
ACCTTCCCTG ATCAGACAGT TAAGAATATC TACCCTGATG GGAGAGAGGA GAGCGTACTG 
GCAGACGGAA CAATAATACA GCAAAACCCG GATGGCAGTA AGAAGATCCA GTTTAACAAC 
GGTCAGCGGG AACTTCACAC TGCTGAATTC AAACGCCGAG AATATCCTGA CGGTACGGTC 
AAAACAGTTT ATTCAGATGG CCGCCAGGAA ACACGATATC CCAGTGGCCG CGTTCGACTC 
AAAGACCCTC AGGGACGTGT TGTTATGGAC GCCAAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007260648.1
CDS	136..3717
Translation	MSSPAGPQPSESRFLSRWMPSSSRAGVFLSGSPGEVSSVQSSCSSLAQEISMEDSFCSQFAPLPVSCSSSLDSLVPKVSEIDPQRKATSSNPDSVQDILTRVQELPLQARLEQLKQLQQRMQEQLKAHQLEQLQKLHNEQQGIVGMVQTGAGYTQEKQQYSRSTSSTLSTAQHSALPTHQEEVLSELESSVWNHESSVVQSSFGQDGDGDEDDMIKGSLQSVIENPSMCRQDRESEPQDRPLKSATSGKTFEEMLEEQLKLENEKINKNAPAEPAKVKRPFLRRGEGLARFKRDKASVTFKEGTARPSTKPTSCPNPVKSQGQALASRHQNSEPKRPSPKPTPTNTVIQRKTAVLNKENLPQSKSLLPASKTVVKAHILGSHQGQNMGSGAVQQLGRSARRPDPPQKIVCDAVEDVNKGPSPGHLRDPSTCVAENSFEVWLAERGDRWEKDNQRECVELGEFELLERAADELSFSSNSSFICTLLQGDRRRLSSTPVKSPPKPKLPGHIQADSRNPVQPSISSVQQGVPDMPVASVAVNIKKSVVVSQYSEEEDEGSKIDDDEDSDASLCSTTEFQQTAFGPGQPFSHCFQISQVPPYNKSSYQDREEGAASPLEEEESNGDSTIVDAKDHIDFDDDDTWNEPEDLTCISAKMDDHAGTTLKRKVAFSKGPELDPSADSSLGREPEPLPTCQLVAKLFPALKPKPCPPAPPVAKNPEATCDEQGAAQSRLLRERLVELETEIERFKTENAVLSRLRQENEDVRENLRKETAQFQQKIADELAKWEEFKREESRKLQREKKLFEKHAAAARARPDKQERDEMQSLKQQLNSLQEDLRKREARWSSTHQRLRQQVDTLSAENTTLRGQVRMLEKLRLTAWKNAESEREKEKERGRSNAPGGGGGSTTSVTAQRRAKSKSPSHSVKTSSTSSKRESPELQNPHKIQACKSRFKPTLSEKEACPVADPYHISDGQPEPEGRSHDSLPINESSLEVESPRAVTEQLDMEQEEITHTDGKIEKLLPDGGRLIVFPNGTRKELSADGLSIKVTFFNGDIKHIMPDQRVIYYYAGAQTTHTTYPDGIEVLQFPNNQIEKHFPDGHKEITFPDQTVKNIYPDGREESVLADGTIIQQNPDGSKKIQFNNGQRELHTAEFKRREYPDGTVKTVYSDGRQETRYPSGRVRLKDPQGRVVMDAKP

Target ORF information:

RefSeq Version	XM_007260586.2
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus centromere protein J (cenpj), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007260586.2

ORF Insert Sequence:

ATGTCATCTC CAGCTGGACC ACAGCCATCC GAGTCTCGCT TCCTGTCCCG CTGGATGCCC 
AGCAGCTCTC GGGCCGGGGT CTTCCTGAGT GGCTCTCCAG GAGAGGTCAG CTCTGTCCAG 
AGCAGCTGCT CTTCTCTGGC TCAGGAGATA TCTATGGAAG ACTCTTTCTG CTCCCAGTTC 
GCCCCTCTGC CTGTGTCCTG CAGCAGCAGC CTGGATTCAT TGGTGCCAAA GGTGTCCGAA 
ATAGACCCGC AGAGAAAAGC AACAAGCAGC AACCCAGACA GTGTCCAGGA CATTTTAACA 
AGAGTACAGG AACTTCCACT GCAGGCGAGG CTTGAGCAGC TAAAGCAGTT ACAGCAGCGC 
ATGCAGGAGC AGCTAAAAGC CCATCAGTTA GAGCAGCTGC AAAAACTGCA CAATGAACAG 
CAGGGCATTG TGGGGATGGT ACAGACAGGC GCAGGATACA CCCAAGAAAA ACAGCAGTAC 
AGCAGAAGCA CATCATCTAC ACTTTCCACT GCACAACACA GTGCTTTGCC TACACACCAG 
GAAGAAGTGC TGAGTGAACT TGAGTCAAGT GTGTGGAATC ATGAATCGAG TGTGGTGCAG 
TCGAGCTTTG GGCAAGATGG TGATGGAGAT GAAGATGATA TGATCAAAGG TTCACTGCAG 
TCTGTAATTG AGAATCCAAG CATGTGCAGA CAAGATAGGG AATCAGAGCC TCAAGACAGG 
CCACTCAAAT CAGCTACCAG TGGGAAGACA TTTGAAGAGA TGTTGGAAGA ACAGCTGAAG 
TTGGAGAATG AGAAGATAAA CAAGAACGCT CCTGCTGAAC CAGCAAAGGT CAAGAGGCCG 
TTTCTGAGAC GTGGGGAAGG TCTGGCCAGG TTTAAAAGAG ATAAAGCCTC CGTAACTTTC 
AAAGAAGGAA CAGCTCGGCC AAGCACCAAA CCCACTTCTT GCCCGAATCC TGTGAAGTCT 
CAGGGCCAAG CACTTGCATC CCGTCACCAA AACTCAGAGC CAAAAAGACC TTCGCCTAAA 
CCTACACCAA CTAATACTGT AATCCAACGC AAGACTGCAG TTCTGAACAA AGAGAACTTG 
CCTCAGAGCA AGTCTTTACT ACCAGCAAGC AAAACTGTTG TCAAAGCTCA TATCCTTGGA 
TCCCACCAGG GTCAGAACAT GGGCTCTGGA GCTGTCCAGC AACTTGGCAG GAGTGCTCGG 
AGGCCTGATC CTCCGCAGAA GATTGTCTGT GATGCTGTGG AGGATGTAAA TAAGGGGCCA 
TCACCTGGGC ATCTCCGGGA TCCTAGTACA TGTGTGGCGG AGAACTCCTT TGAAGTGTGG 
CTTGCAGAGC GCGGGGATCG TTGGGAAAAG GATAATCAAC GTGAGTGTGT GGAGCTTGGT 
GAGTTTGAGC TGCTGGAGAG AGCAGCGGAT GAACTATCAT TCTCAAGCAA CTCTTCCTTT 
ATTTGTACTC TTCTTCAGGG AGATCGTAGA CGTCTGTCCT CAACGCCAGT CAAATCACCT 
CCAAAACCTA AGTTGCCTGG TCATATTCAA GCTGACTCTA GAAACCCAGT GCAGCCGAGC 
ATTAGCAGTG TCCAGCAGGG TGTACCAGAC ATGCCTGTAG CCAGTGTTGC AGTAAACATT 
AAGAAGAGTG TGGTGGTCAG CCAGTATTCG GAGGAGGAAG ATGAGGGTAG TAAAATAGAT 
GATGATGAAG ATTCTGATGC TTCATTGTGT AGCACTACAG AGTTCCAACA AACTGCATTT 
GGACCAGGTC AGCCTTTCAG CCACTGCTTT CAAATATCTC AGGTCCCGCC CTACAATAAA 
AGCTCCTACC AGGACAGGGA GGAAGGAGCT GCCAGTCCAC TCGAGGAAGA GGAAAGCAAT 
GGAGATTCTA CTATAGTGGA TGCCAAGGAT CACATTGACT TTGATGACGA TGACACTTGG 
AATGAGCCTG AGGACTTGAC CTGCATTTCC GCTAAGATGG ACGATCATGC AGGCACGACA 
CTGAAGAGGA AGGTTGCCTT CTCAAAAGGA CCAGAGCTTG ACCCTTCTGC TGACAGCTCA 
CTGGGCCGTG AACCAGAGCC TCTGCCCACA TGCCAGTTGG TAGCAAAGCT GTTCCCAGCC 
CTCAAGCCGA AGCCCTGCCC TCCAGCTCCT CCAGTTGCAA AGAATCCAGA AGCAACATGC 
GACGAACAAG GAGCAGCCCA GTCCCGCTTA CTTCGTGAGC GTTTGGTGGA GCTGGAAACA 
GAGATTGAGA GGTTCAAGAC AGAAAATGCG GTGTTGAGCA GACTCAGACA GGAAAACGAG 
GATGTCAGAG AGAACCTGAG GAAAGAGACA GCCCAGTTTC AGCAGAAGAT TGCAGATGAA 
CTTGCCAAAT GGGAAGAGTT CAAGCGCGAG GAAAGCCGGA AGCTGCAGCG GGAAAAGAAG 
CTGTTTGAGA AGCATGCGGC AGCTGCCAGG GCGAGACCTG ACAAACAGGA GCGAGATGAG 
ATGCAGTCTC TAAAGCAGCA ACTGAACTCT CTGCAAGAGG ATTTAAGAAA ACGAGAAGCT 
CGCTGGTCCA GCACCCATCA ACGCCTCCGG CAGCAGGTAG ACACCCTGAG TGCTGAGAAC 
ACGACTCTCC GAGGCCAGGT CCGCATGCTG GAGAAACTCC GCCTGACTGC TTGGAAAAAT 
GCTGAGAGTG AGCGTGAAAA AGAGAAAGAG AGGGGAAGGT CTAATGCACC TGGTGGTGGT 
GGCGGCAGCA CCACCAGTGT CACAGCTCAG AGAAGGGCCA AATCCAAGAG TCCTTCCCAC 
AGTGTAAAGA CCAGCAGCAC GAGCAGCAAA AGGGAAAGCC CAGAGCTTCA GAATCCTCAT 
AAAATTCAAG CTTGTAAATC AAGGTTTAAG CCTACTCTGA GTGAAAAGGA AGCCTGCCCG 
GTGGCTGATC CATACCACAT ATCTGATGGA CAACCTGAGC CTGAAGGGAG GAGCCATGAT 
TCTCTTCCAA TCAATGAATC CTCTTTAGAG GTGGAAAGTC CTAGAGCTGT TACAGAGCAG 
CTGGATATGG AACAAGAAGA AATCACGCAC ACTGATGGCA AGATTGAAAA GCTTCTTCCA 
GATGGAGGGA GACTTATAGT GTTCCCTAAC GGCACAAGGA AAGAGCTGTC TGCAGACGGG 
CTGTCGATCA AAGTCACTTT TTTTAATGGA GACATCAAAC ACATCATGCC TGATCAAAGA 
GTGATTTATT ACTATGCAGG AGCTCAGACC ACACATACGA CCTACCCTGA TGGCATAGAA 
GTTCTCCAGT TCCCTAATAA TCAGATAGAA AAACACTTCC CTGATGGCCA TAAGGAGATC 
ACCTTCCCTG ATCAGACAGT TAAGAATATC TACCCTGATG GGAGAGAGGA GAGCGTACTG 
GCAGACGGAA CAATAATACA GCAAAACCCG GATGGCAGTA AGAAGATCCA GTTTAACAAC 
GGTCAGCGGG AACTTCACAC TGCTGAATTC AAACGCCGAG AATATCCTGA CGGTACGGTC 
AAAACAGTTT ATTCAGATGG CCGCCAGGAA ACACGATATC CCAGTGGCCG CGTTCGACTC 
AAAGACCCTC AGGGACGTGT TGTTATGGAC GCCAAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cenpj ( XM_015608668.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_015464154.1 Astyanax mexicanus centromere protein J (cenpj), transcript variant X3, mRNA.

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CloneID	OAs42148
Clone ID Related Accession (Same CDS sequence)	XM_015608668.1
Accession Version	XM_015608668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	centromere protein J isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749487.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCTC CAGCTGGACC ACAGCCATCC GAGTCTCGCT TCCTGTCCCG CTGGATGCCC 
AGCAGCTCTC GGGCCGGGGT CTTCCTGAGT GGCTCTCCAG GAGAGGTCAG CTCTGTCCAG 
AGCAGCTGCT CTTCTCTGGC TCAGGAGATA TCTATGGAAG ACTCTTTCTG CTCCCAGTTC 
GCCCCTCTGC CTGTGTCCTG CAGCAGCAGC CTGGATTCAT TGGTGCCAAA GGTGTCCGAA 
ATAGACCCGC AGAGAAAAGC AACAAGCAGC AACCCAGACA GTGTCCAGGA CATTTTAACA 
AGAGTACAGG AACTTCCACT GCAGGCGAGG CTTGAGCAGC TAAAGCAGTT ACAGCAGCGC 
ATGCAGGAGC AGCTAAAAGC CCATCAGTTA GAGCAGCTGC AAAAACTGCA CAATGAACAG 
CAGGGCATTG TGGGGATGGT ACAGACAGGC GCAGAGTCAA GTGTGTGGAA TCATGAATCG 
AGTGTGGTGC AGTCGAGCTT TGGGCAAGAT GGTGATGGAG ATGAAGATGA TATGATCAAA 
GGTTCACTGC AGTCTGTAAT TGAGAATCCA AGCATGTGCA GACAAGATAG GGAATCAGAG 
CCTCAAGACA GGCCACTCAA ATCAGCTACC AGTGGGAAGA CATTTGAAGA GATGTTGGAA 
GAACAGCTGA AGTTGGAGAA TGAGAAGATA AACAAGAACG CTCCTGCTGA ACCAGCAAAG 
GTCAAGAGGC CGTTTCTGAG ACGTGGGGAA GGTCTGGCCA GGTTTAAAAG AGATAAAGCC 
TCCGTAACTT TCAAAGAAGG AACAGCTCGG CCAAGCACCA AACCCACTTC TTGCCCGAAT 
CCTGTGAAGT CTCAGGGCCA AGCACTTGCA TCCCGTCACC AAAACTCAGA GCCAAAAAGA 
CCTTCGCCTA AACCTACACC AACTAATACT GTAATCCAAC GCAAGACTGC AGTTCTGAAC 
AAAGAGAACT TGCCTCAGAG CAAGTCTTTA CTACCAGCAA GCAAAACTGT TGTCAAAGCT 
CATATCCTTG GATCCCACCA GGGTCAGAAC ATGGGCTCTG GAGCTGTCCA GCAACTTGGC 
AGGAGTGCTC GGAGGCCTGA TCCTCCGCAG AAGATTGTCT GTGATGCTGT GGAGGATGTA 
AATAAGGGGC CATCACCTGG GCATCTCCGG GATCCTAGTA CATGTGTGGC GGAGAACTCC 
TTTGAAGTGT GGCTTGCAGA GCGCGGGGAT CGTTGGGAAA AGGATAATCA ACGTGAGTGT 
GTGGAGCTTG GTGAGTTTGA GCTGCTGGAG AGAGCAGCGG ATGAACTATC ATTCTCAAGC 
AACTCTTCCT TTATTTGTAC TCTTCTTCAG GGAGATCGTA GACGTCTGTC CTCAACGCCA 
GTCAAATCAC CTCCAAAACC TAAGTTGCCT GGTCATATTC AAGCTGACTC TAGAAACCCA 
GTGCAGCCGA GCATTAGCAG TGTCCAGCAG GGTGTACCAG ACATGCCTGT AGCCAGTGTT 
GCAGTAAACA TTAAGAAGAG TGTGGTGGTC AGCCAGTATT CGGAGGAGGA AGATGAGGGT 
AGTAAAATAG ATGATGATGA AGATTCTGAT GCTTCATTGT GTAGCACTAC AGAGTTCCAA 
CAAACTGCAT TTGGACCAGG TCAGCCTTTC AGCCACTGCT TTCAAATATC TCAGGTCCCG 
CCCTACAATA AAAGCTCCTA CCAGGACAGG GAGGAAGGAG CTGCCAGTCC ACTCGAGGAA 
GAGGAAAGCA ATGGAGATTC TACTATAGTG GATGCCAAGG ATCACATTGA CTTTGATGAC 
GATGACACTT GGAATGAGCC TGAGGACTTG ACCTGCATTT CCGCTAAGAT GGACGATCAT 
GCAGGCACGA CACTGAAGAG GAAGGTTGCC TTCTCAAAAG GACCAGAGCT TGACCCTTCT 
GCTGACAGCT CACTGGGCCG TGAACCAGAG CCTCTGCCCA CATGCCAGTT GGTAGCAAAG 
CTGTTCCCAG CCCTCAAGCC GAAGCCCTGC CCTCCAGCTC CTCCAGTTGC AAAGAATCCA 
GAAGCAACAT GCGACGAACA AGGAGCAGCC CAGTCCCGCT TACTTCGTGA GCGTTTGGTG 
GAGCTGGAAA CAGAGATTGA GAGGTTCAAG ACAGAAAATG CGGTGTTGAG CAGACTCAGA 
CAGGAAAACG AGGATGTCAG AGAGAACCTG AGGAAAGAGA CAGCCCAGTT TCAGCAGAAG 
ATTGCAGATG AACTTGCCAA ATGGGAAGAG TTCAAGCGCG AGGAAAGCCG GAAGCTGCAG 
CGGGAAAAGA AGCTGTTTGA GAAGCATGCG GCAGCTGCCA GGGCGAGACC TGACAAACAG 
GAGCGAGATG AGATGCAGTC TCTAAAGCAG CAACTGAACT CTCTGCAAGA GGATTTAAGA 
AAACGAGAAG CTCGCTGGTC CAGCACCCAT CAACGCCTCC GGCAGCAGGT AGACACCCTG 
AGTGCTGAGA ACACGACTCT CCGAGGCCAG GTCCGCATGC TGGAGAAACT CCGCCTGACT 
GCTTGGAAAA ATGCTGAGAG TGAGCGTGAA AAAGAGAAAG AGAGGGGAAG GTCTAATGCA 
CCTGGTGGTG GTGGCGGCAG CACCACCAGT GTCACAGCTC AGAGAAGGGC CAAATCCAAG 
AGTCCTTCCC ACAGTGTAAA GACCAGCAGC ACGAGCAGCA AAAGGGAAAG CCCAGAGCTT 
CAGAATCCTC ATAAAATTCA AGCTTGTAAA TCAAGGTTTA AGCCTACTCT GAGTGAAAAG 
GAAGCCTGCC CGGTGGCTGA TCCATACCAC ATATCTGATG GACAACCTGA GCCTGAAGGG 
AGGAGCCATG ATTCTCTTCC AATCAATGAA TCCTCTTTAG AGGTGGAAAG TCCTAGAGCT 
GTTACAGAGC AGCTGGATAT GGAACAAGAA GAAATCACGC ACACTGATGG CAAGATTGAA 
AAGCTTCTTC CAGATGGAGG GAGACTTATA GTGTTCCCTA ACGGCACAAG GAAAGAGCTG 
TCTGCAGACG GGCTGTCGAT CAAAGTCACT TTTTTTAATG GAGACATCAA ACACATCATG 
CCTGATCAAA GAGTGATTTA TTACTATGCA GGAGCTCAGA CCACACATAC GACCTACCCT 
GATGGCATAG AAGTTCTCCA GTTCCCTAAT AATCAGATAG AAAAACACTT CCCTGATGGC 
CATAAGGAGA TCACCTTCCC TGATCAGACA GTTAAGAATA TCTACCCTGA TGGGAGAGAG 
GAGAGCGTAC TGGCAGACGG AACAATAATA CAGCAAAACC CGGATGGCAG TAAGAAGATC 
CAGTTTAACA ACGGTCAGCG GGAACTTCAC ACTGCTGAAT TCAAACGCCG AGAATATCCT 
GACGGTACGG TCAAAACAGT TTATTCAGAT GGCCGCCAGG AAACACGATA TCCCAGTGGC 
CGCGTTCGAC TCAAAGACCC TCAGGGACGT GTTGTTATGG ACGCCAAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015464154.1
CDS	136..3609
Translation	MSSPAGPQPSESRFLSRWMPSSSRAGVFLSGSPGEVSSVQSSCSSLAQEISMEDSFCSQFAPLPVSCSSSLDSLVPKVSEIDPQRKATSSNPDSVQDILTRVQELPLQARLEQLKQLQQRMQEQLKAHQLEQLQKLHNEQQGIVGMVQTGAESSVWNHESSVVQSSFGQDGDGDEDDMIKGSLQSVIENPSMCRQDRESEPQDRPLKSATSGKTFEEMLEEQLKLENEKINKNAPAEPAKVKRPFLRRGEGLARFKRDKASVTFKEGTARPSTKPTSCPNPVKSQGQALASRHQNSEPKRPSPKPTPTNTVIQRKTAVLNKENLPQSKSLLPASKTVVKAHILGSHQGQNMGSGAVQQLGRSARRPDPPQKIVCDAVEDVNKGPSPGHLRDPSTCVAENSFEVWLAERGDRWEKDNQRECVELGEFELLERAADELSFSSNSSFICTLLQGDRRRLSSTPVKSPPKPKLPGHIQADSRNPVQPSISSVQQGVPDMPVASVAVNIKKSVVVSQYSEEEDEGSKIDDDEDSDASLCSTTEFQQTAFGPGQPFSHCFQISQVPPYNKSSYQDREEGAASPLEEEESNGDSTIVDAKDHIDFDDDDTWNEPEDLTCISAKMDDHAGTTLKRKVAFSKGPELDPSADSSLGREPEPLPTCQLVAKLFPALKPKPCPPAPPVAKNPEATCDEQGAAQSRLLRERLVELETEIERFKTENAVLSRLRQENEDVRENLRKETAQFQQKIADELAKWEEFKREESRKLQREKKLFEKHAAAARARPDKQERDEMQSLKQQLNSLQEDLRKREARWSSTHQRLRQQVDTLSAENTTLRGQVRMLEKLRLTAWKNAESEREKEKERGRSNAPGGGGGSTTSVTAQRRAKSKSPSHSVKTSSTSSKRESPELQNPHKIQACKSRFKPTLSEKEACPVADPYHISDGQPEPEGRSHDSLPINESSLEVESPRAVTEQLDMEQEEITHTDGKIEKLLPDGGRLIVFPNGTRKELSADGLSIKVTFFNGDIKHIMPDQRVIYYYAGAQTTHTTYPDGIEVLQFPNNQIEKHFPDGHKEITFPDQTVKNIYPDGREESVLADGTIIQQNPDGSKKIQFNNGQRELHTAEFKRREYPDGTVKTVYSDGRQETRYPSGRVRLKDPQGRVVMDAKP

Target ORF information:

RefSeq Version	XM_015608668.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus centromere protein J (cenpj), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015608668.1

ORF Insert Sequence:

ATGTCATCTC CAGCTGGACC ACAGCCATCC GAGTCTCGCT TCCTGTCCCG CTGGATGCCC 
AGCAGCTCTC GGGCCGGGGT CTTCCTGAGT GGCTCTCCAG GAGAGGTCAG CTCTGTCCAG 
AGCAGCTGCT CTTCTCTGGC TCAGGAGATA TCTATGGAAG ACTCTTTCTG CTCCCAGTTC 
GCCCCTCTGC CTGTGTCCTG CAGCAGCAGC CTGGATTCAT TGGTGCCAAA GGTGTCCGAA 
ATAGACCCGC AGAGAAAAGC AACAAGCAGC AACCCAGACA GTGTCCAGGA CATTTTAACA 
AGAGTACAGG AACTTCCACT GCAGGCGAGG CTTGAGCAGC TAAAGCAGTT ACAGCAGCGC 
ATGCAGGAGC AGCTAAAAGC CCATCAGTTA GAGCAGCTGC AAAAACTGCA CAATGAACAG 
CAGGGCATTG TGGGGATGGT ACAGACAGGC GCAGAGTCAA GTGTGTGGAA TCATGAATCG 
AGTGTGGTGC AGTCGAGCTT TGGGCAAGAT GGTGATGGAG ATGAAGATGA TATGATCAAA 
GGTTCACTGC AGTCTGTAAT TGAGAATCCA AGCATGTGCA GACAAGATAG GGAATCAGAG 
CCTCAAGACA GGCCACTCAA ATCAGCTACC AGTGGGAAGA CATTTGAAGA GATGTTGGAA 
GAACAGCTGA AGTTGGAGAA TGAGAAGATA AACAAGAACG CTCCTGCTGA ACCAGCAAAG 
GTCAAGAGGC CGTTTCTGAG ACGTGGGGAA GGTCTGGCCA GGTTTAAAAG AGATAAAGCC 
TCCGTAACTT TCAAAGAAGG AACAGCTCGG CCAAGCACCA AACCCACTTC TTGCCCGAAT 
CCTGTGAAGT CTCAGGGCCA AGCACTTGCA TCCCGTCACC AAAACTCAGA GCCAAAAAGA 
CCTTCGCCTA AACCTACACC AACTAATACT GTAATCCAAC GCAAGACTGC AGTTCTGAAC 
AAAGAGAACT TGCCTCAGAG CAAGTCTTTA CTACCAGCAA GCAAAACTGT TGTCAAAGCT 
CATATCCTTG GATCCCACCA GGGTCAGAAC ATGGGCTCTG GAGCTGTCCA GCAACTTGGC 
AGGAGTGCTC GGAGGCCTGA TCCTCCGCAG AAGATTGTCT GTGATGCTGT GGAGGATGTA 
AATAAGGGGC CATCACCTGG GCATCTCCGG GATCCTAGTA CATGTGTGGC GGAGAACTCC 
TTTGAAGTGT GGCTTGCAGA GCGCGGGGAT CGTTGGGAAA AGGATAATCA ACGTGAGTGT 
GTGGAGCTTG GTGAGTTTGA GCTGCTGGAG AGAGCAGCGG ATGAACTATC ATTCTCAAGC 
AACTCTTCCT TTATTTGTAC TCTTCTTCAG GGAGATCGTA GACGTCTGTC CTCAACGCCA 
GTCAAATCAC CTCCAAAACC TAAGTTGCCT GGTCATATTC AAGCTGACTC TAGAAACCCA 
GTGCAGCCGA GCATTAGCAG TGTCCAGCAG GGTGTACCAG ACATGCCTGT AGCCAGTGTT 
GCAGTAAACA TTAAGAAGAG TGTGGTGGTC AGCCAGTATT CGGAGGAGGA AGATGAGGGT 
AGTAAAATAG ATGATGATGA AGATTCTGAT GCTTCATTGT GTAGCACTAC AGAGTTCCAA 
CAAACTGCAT TTGGACCAGG TCAGCCTTTC AGCCACTGCT TTCAAATATC TCAGGTCCCG 
CCCTACAATA AAAGCTCCTA CCAGGACAGG GAGGAAGGAG CTGCCAGTCC ACTCGAGGAA 
GAGGAAAGCA ATGGAGATTC TACTATAGTG GATGCCAAGG ATCACATTGA CTTTGATGAC 
GATGACACTT GGAATGAGCC TGAGGACTTG ACCTGCATTT CCGCTAAGAT GGACGATCAT 
GCAGGCACGA CACTGAAGAG GAAGGTTGCC TTCTCAAAAG GACCAGAGCT TGACCCTTCT 
GCTGACAGCT CACTGGGCCG TGAACCAGAG CCTCTGCCCA CATGCCAGTT GGTAGCAAAG 
CTGTTCCCAG CCCTCAAGCC GAAGCCCTGC CCTCCAGCTC CTCCAGTTGC AAAGAATCCA 
GAAGCAACAT GCGACGAACA AGGAGCAGCC CAGTCCCGCT TACTTCGTGA GCGTTTGGTG 
GAGCTGGAAA CAGAGATTGA GAGGTTCAAG ACAGAAAATG CGGTGTTGAG CAGACTCAGA 
CAGGAAAACG AGGATGTCAG AGAGAACCTG AGGAAAGAGA CAGCCCAGTT TCAGCAGAAG 
ATTGCAGATG AACTTGCCAA ATGGGAAGAG TTCAAGCGCG AGGAAAGCCG GAAGCTGCAG 
CGGGAAAAGA AGCTGTTTGA GAAGCATGCG GCAGCTGCCA GGGCGAGACC TGACAAACAG 
GAGCGAGATG AGATGCAGTC TCTAAAGCAG CAACTGAACT CTCTGCAAGA GGATTTAAGA 
AAACGAGAAG CTCGCTGGTC CAGCACCCAT CAACGCCTCC GGCAGCAGGT AGACACCCTG 
AGTGCTGAGA ACACGACTCT CCGAGGCCAG GTCCGCATGC TGGAGAAACT CCGCCTGACT 
GCTTGGAAAA ATGCTGAGAG TGAGCGTGAA AAAGAGAAAG AGAGGGGAAG GTCTAATGCA 
CCTGGTGGTG GTGGCGGCAG CACCACCAGT GTCACAGCTC AGAGAAGGGC CAAATCCAAG 
AGTCCTTCCC ACAGTGTAAA GACCAGCAGC ACGAGCAGCA AAAGGGAAAG CCCAGAGCTT 
CAGAATCCTC ATAAAATTCA AGCTTGTAAA TCAAGGTTTA AGCCTACTCT GAGTGAAAAG 
GAAGCCTGCC CGGTGGCTGA TCCATACCAC ATATCTGATG GACAACCTGA GCCTGAAGGG 
AGGAGCCATG ATTCTCTTCC AATCAATGAA TCCTCTTTAG AGGTGGAAAG TCCTAGAGCT 
GTTACAGAGC AGCTGGATAT GGAACAAGAA GAAATCACGC ACACTGATGG CAAGATTGAA 
AAGCTTCTTC CAGATGGAGG GAGACTTATA GTGTTCCCTA ACGGCACAAG GAAAGAGCTG 
TCTGCAGACG GGCTGTCGAT CAAAGTCACT TTTTTTAATG GAGACATCAA ACACATCATG 
CCTGATCAAA GAGTGATTTA TTACTATGCA GGAGCTCAGA CCACACATAC GACCTACCCT 
GATGGCATAG AAGTTCTCCA GTTCCCTAAT AATCAGATAG AAAAACACTT CCCTGATGGC 
CATAAGGAGA TCACCTTCCC TGATCAGACA GTTAAGAATA TCTACCCTGA TGGGAGAGAG 
GAGAGCGTAC TGGCAGACGG AACAATAATA CAGCAAAACC CGGATGGCAG TAAGAAGATC 
CAGTTTAACA ACGGTCAGCG GGAACTTCAC ACTGCTGAAT TCAAACGCCG AGAATATCCT 
GACGGTACGG TCAAAACAGT TTATTCAGAT GGCCGCCAGG AAACACGATA TCCCAGTGGC 
CGCGTTCGAC TCAAAGACCC TCAGGGACGT GTTGTTATGG ACGCCAAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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cenpj cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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