brca1 cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene
Clones
gRNAs

Summary

Gene Symbol	brca1
Entrez Gene ID	103023552
Full Name	BRCA1, DNA repair associated
Gene Type	protein-coding
Organism	Astyanax mexicanus(Mexican tetra)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015602449.1	XP_015457935.1	breast cancer type 1 susceptibility protein
XM_022669405.1	XP_022525126.1	breast cancer type 1 susceptibility protein isoform X3
XM_022669403.1	XP_022525124.1	breast cancer type 1 susceptibility protein isoform X1
XM_022669404.1	XP_022525125.1	breast cancer type 1 susceptibility protein isoform X2

Pathways from BioSystems
Gene Ontology

The following brca1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brca1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAs125386	XM_022669403.1 Latest version!	Astyanax mexicanus BRCA1, DNA repair associated (brca1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAs125387	XM_022669404.1 Latest version!	Astyanax mexicanus BRCA1, DNA repair associated (brca1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAs125388	XM_022669405.1 Latest version!	Astyanax mexicanus BRCA1, DNA repair associated (brca1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAs21638	XM_015602449.1 Latest version!	Astyanax mexicanus breast cancer 1 (brca1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

brca1 ( XM_022669403.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_022525124.1 Astyanax mexicanus BRCA1, DNA repair associated (brca1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAs125386
Clone ID Related Accession (Same CDS sequence)	XM_022669403.1
Accession Version	XM_022669403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	breast cancer type 1 susceptibility protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCTC CCAAAGCCGA GGACATCAAG CGGGGAATTG CTGTGATATG GGAGAATTTG 
CAGTGTCCAA TATGTTTGGA CTTGATGAAT GCACCAGTAT CGACTAGGTG TGATCACCAG 
TTCTGCAAGT TCTGCCTGAT GAAACTGCTG GAAAGGAGTA AAAAGAAAGA GACGAGCTGC 
CCTGTGTGCA AGATGAATGT CACTAAAAGG AGTCTGAAAG AAAGTCCAGG GTTCCAGAGG 
CTGGTGGAAG GACTGCAGAA TTTAGTCCAG TCTTATGAAT TTGACACCTG TACAAGCTAT 
TTTACAGGAA TACCACAGAG CAGGAAAGAA ACCAGTGTGG AAACAGAGTC CAGAGATCAG 
CAGTGCAGTG AGGAAAATGG CTCCTCAGGG AACGATGTGA CAGATAAGTC AGCATCTCAT 
TTCTCCTCGG CTGCAGTGAA GGATGCATTT GCAGAACTCA TGGGTCTTGT GGATTCCTGT 
ACAGTTAATT CAGAGCAGGA CTGTTTTGAC AGTGGTCTTG GCGGTCCTGT TAATAAAGCA 
GATGAGCTTC AAGATGTTCC TTCAAGAATA GAAGACCGAC AGAGTAATTC ACCCATACCT 
GATAGCAGCG GTTCTGATGG TAAGACTGAC CTACACAAGA GACGGTCAAG CAGAAGACCA 
AAAAGAGTAG GCTTGGAGCC AGACAGAATT GCAGACAAAA GACAAAAGAA AAGTGTGGAA 
AAAGTAGCAG AGTGGTTGCT AAAGATTTCA CCTTCATCAG ACACACAAAC CAGAGACAGG 
AGTAGAGATC CCTCAGTTCC CGCTGATAGT GATTCTGAAA AGGAGAGTGT GTGTTCATCC 
TCCCCTACAG AGGCTTATCC GAAAGATGGC GATGAGGATG ACGTCAATCC ACCAAAGGAG 
GTGCGTGGAC GTGGTCTAGA GGAAAAGGTT TTTGGGGCTG TCTATAAACG TGAACGTAAA 
GGTGGTAAAA CCAAAGTAAA CAGACCTGTC TCTCCTGAAA AAGAGACCGT GACCAACCCT 
GTTCCCTTTA ATTTGGTGCA AGAGGAAGAA ATGATGACAC GACATGGCCT TAAGAGAAAG 
AGGAGCTGTA GGCTGACTCC GGCAGATTTC ATTAAGAAAT CATCTTCTGA CGGTAGAGAA 
GCAGAAGTTA AAGAGCCCGT GGACTCTGTT ACTCATAATG CTGTGGAAGA AAACAAGCAA 
GTTGAATTAA ATACTGAGGA ACAAATAGAA ATTTTGGGAG AGTGTGACAA GATAGCAGAG 
CTCTCTGCAG ATGCAAAATG TGACAATACA GTTAAAAACA TGGAAGAAAG TCCTGCGATT 
GAAGTACCTT TGAAGAGATC AGGGAGAAGA TCAAAGTCAA AAATGCAGGA TATATGGCAA 
AATGTGGATC TTGACTTAAA AGAAAAAGAA AACATTTGTG ACAATAGAAC AAGTCGCAAG 
AGGCGGAGCA ATAGGAGCAG CTGTGAGCTG ACAAAAGATG GCCACTTGGA TGAAAGCAAG 
AATGCAAAGT GTACAAAATC CCTTACCCTT GTGGATGCGG CTGCTAATAA TGTTGATGTA 
ATGGAGCAGT TGAAAAATAA ACCAAAAATA GTGGAAGCAG AAATTAATAT TGAGAGTTAC 
CCAAGCAGTG CAGATCCACG ATCTCCTGAT GCTAGAAAAA CAAGAAGAAG TTTAAGGCTT 
CAGGCCTTCA CTACAGAAAT TCAGGGAACT CTGAAACAGA GACGAACTAC AAAACCTTCC 
AGTGGACCTG AAACTCATCC GTTAGATAAT CCATCACACT TTAATTGTTT GGACCAACCT 
AATGTGGATA AAGTGAATGC AAATGAAGAA AATGTTGATG CTGATCAGGG TATTGGAAGT 
ACTCAACAGT CTCGCAGTGG AAAAACTGAC CTGCTGATCA TGAAAAATGG ATGTGTATTC 
AACAGCAGCT TAGGAACAAT TGAGGCAGTG CAGACTAATG AGGAAACAGC TACTTCTCCA 
TGTGTTTCAG AGGGACAAGG CCCTGAACAA AATTCTGTCC TGTCCATTAT TCCAGACACA 
GTGGCTCAAG ATGGACCAAG CCTTCCTTGT TCTTCTGTGG CTGCCAGCTC TGCATTTCCA 
TTACAGGCTG TCACACTAGA CTCATCCTTA CCAGAGAAAA GCCAGACGTC TCCATCCGCA 
GGGTTCGTCG CCCCGTCTGT TGTACAGACA TTGAAAGATG CTCATTGTGA GCGTGGAGGC 
ATAGAAGTGG AAGGTGAGAC CAATGATAGT GAACTTGATA CAGAGCTTCT AATGAAGTCC 
TTCAAAGCTT CCAAAAGAAA ATCTTTCCAC CTGGGCAGCC CCAAGCCTTC ACCTAAAACA 
GTCCAGGAGA GGCTTCTAAC CAGGACTGAC GAGGAACAGA AGCTGCAATA TCAGATACCA 
ACCACTTGTA AAGACGATGA ACTAACATCA GATCTTGCAA CCATTTGCCA TAATGACTTG 
CCTCCATCTT CAAATGCTTC TAAGCTTCCG TTATGTGAGG AATCGTCTCA AAGGTCTCAG 
AGCAGTTTTG GTAATGCAGC TGTTGCAAGT CTTATCCAAG CAAATGCATC TGTTGCCACA 
GGAGATCAAA AGGAAGCTTT TCTGAAGAAC TCCGGCAACA GCACATGTTC AGGCCTAAGT 
CCCAACAGAG TTGCAAGATC TTCTCAAGAT TCCAAGCTTC CTACAAATTC TGACTCTGCA 
GCATCCTACC AGGGGTTCTT TCCTAAAGTT ACTTCAGAGG AGTCATCAAT CTGCAGCAGC 
AGCGAAGTCC AAAAGAATGA GCTCCAGAAA TCTCCAAAAT TAGGTACTTG TCAACCAAGG 
ATTGCCGAGA TGTCAAACAA AGTCGAGGGC AGCGATGTGC AGACAAAACA TTCTTCAGAC 
TCAAACAAAA GCCCAAACAT CGCAGCAGCA GGTTCACCAA ATCTTGGAGC TCACAGTAAT 
CAATTTGAAT CCTCAATCAC CCCAGATGGA CTCATACCAA AAGGTCCTGT TCCTTATGAA 
TGTCAAGTCA CAGAACCTGC ATCTAATCAC AGTTTGAATG AGGAAAATGA TGCAGGGTCT 
CAGCCGTGCG TGCCGAGAAA GAGGAGGGCT CAGAGGCTCG AGTCCTCAGA GTCTGAGCTC 
AGTGTTGAAG ATGATTCTCT TCCAACACTG GCTCATATAT TCAAATCTCA CCGTTTACCC 
TCACACTCTG GGCAGGAACC AAATCAGACC TCTGAGATTT CTGAGTTAGA TCACCAAGAA 
CCAGATCCAG GCTATCAATC TCCAGCTGAC CAAGAACCAG ATCCAGGCTA TCAATCTCCA 
GCTGACCAAG AACCAGATCC AGGCTTTCAA TCTCCAGCTG ACCAGGCTAA CAAATCAAAT 
GAAGCAACAC CAGAGGCACA GTCTCAGAAT CAGGAATGTC CTGAAAATGC AAATGCTACA 
GCAGACGATG ACCATCTACC TCAACCTTCC TGCAGAGAGG AATGGGTTAC ATCGAGCCAA 
GGATCTGTGG ATCTCTTCGG GACGCCAGAA GAAAGTGAAG CCGTTGGTAT CTGTGGAAAT 
GTTGCAGTTT CCAGGGATTC CTCTCAGTAC TCGAGTGAGA TTATCACCAC ACAGCAGAAA 
GAGGAGATGC AGCAGGAGCT GTGTAGGCTG GAGAGGATGA TGGCTCTGGT GTCTGAGGCT 
CTCCAGAAGA AGGAGAAGGA CACAAGCGCC AAAACCCAGA ACAAGGCTCT CAACCCTACT 
CACTCTGAAC TACAGTCAGC TGCTGACCCT GACCAGGCTG AGCCGTGCAG CCCTGCACTC 
CCTGCAGACA GGCAGAGCGG CCTTGATTCT GACGGAGATC ACAGTGGGGT GTCCCCTGGC 
CCTGCAGCTC CAGACGGCCT GTGTGCTCCC GAGTCCCAAC CTCTGTTGAT GACAGACCAG 
GCAAAGACAG GGCGGCGGGT CAGCAGGAGC CGTCAGAAAT CTCAGGGCAG GGGGAGGAAG 
TCTTCACAGA AGGCAGAAGC TGGGTTATCT CTCAGAAATC ACAACACCAC CTCGGAGGGT 
TCGGTTACGG AGCTGACTAA GGGAAACGAG GCAAAAGAAG CCCAGAAACA AACCCGTGGA 
GCGGACATGC GCAGACTGGG GTCCAGCAGC ACAGCAGGGA AGATGGAGCT GGTGGCTTCT 
GGGCTCAGTG CCAGCGAATT GATGTTGGTG AAGAAATTTG CCAGGAAAAT GCACGGCCAT 
CTAAGTAAAG AGATGACCCC CGGTACTACC CATGTCATCA TTAAGACAGA TGAGGACCTC 
GTGTGTGAGC GCACCTTAAA GTACTTTCAG GGGATCGCCG GCAGGAAGTG GGTGGTGAGC 
TTCCTGTGGA TCTCGGAGTG CTTTAATCAA GGCCAAGTTC TAGACGAGGC TCAGTTTGAA 
GTTCGTGGAG ATGTTGTTAA TGGACACAAC CACAACGGGC CCTTAAAAAG CCGGACCACC 
TCTGATGGGT TGCTGATGAA AGGATACGAG ATCTGCTTCC AGGGATCCTT CACAGCGATG 
ACTACAGATC AGATGGAGGC TATGGTGGAG ATGTGTGGCG CTACTGTTGT AAAAGACCCG 
CTCATGTTCT CCAAACACAT AGAAAGTAAG TGTCAGCTGG TGGTGGTGCA GCCTGGTTCT 
GATGACTCTC AAACTTACTA CAGAGCTCTG CAGAAGAAAG CCACAGTGGT GTCTCGTAGC 
TGGCTCTTGG ATACCATATC AACCTACAGC CTCCAGAATC CCCAGGACCA TAAACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022525124.1
CDS	176..4915
Translation	MTAPKAEDIKRGIAVIWENLQCPICLDLMNAPVSTRCDHQFCKFCLMKLLERSKKKETSCPVCKMNVTKRSLKESPGFQRLVEGLQNLVQSYEFDTCTSYFTGIPQSRKETSVETESRDQQCSEENGSSGNDVTDKSASHFSSAAVKDAFAELMGLVDSCTVNSEQDCFDSGLGGPVNKADELQDVPSRIEDRQSNSPIPDSSGSDGKTDLHKRRSSRRPKRVGLEPDRIADKRQKKSVEKVAEWLLKISPSSDTQTRDRSRDPSVPADSDSEKESVCSSSPTEAYPKDGDEDDVNPPKEVRGRGLEEKVFGAVYKRERKGGKTKVNRPVSPEKETVTNPVPFNLVQEEEMMTRHGLKRKRSCRLTPADFIKKSSSDGREAEVKEPVDSVTHNAVEENKQVELNTEEQIEILGECDKIAELSADAKCDNTVKNMEESPAIEVPLKRSGRRSKSKMQDIWQNVDLDLKEKENICDNRTSRKRRSNRSSCELTKDGHLDESKNAKCTKSLTLVDAAANNVDVMEQLKNKPKIVEAEINIESYPSSADPRSPDARKTRRSLRLQAFTTEIQGTLKQRRTTKPSSGPETHPLDNPSHFNCLDQPNVDKVNANEENVDADQGIGSTQQSRSGKTDLLIMKNGCVFNSSLGTIEAVQTNEETATSPCVSEGQGPEQNSVLSIIPDTVAQDGPSLPCSSVAASSAFPLQAVTLDSSLPEKSQTSPSAGFVAPSVVQTLKDAHCERGGIEVEGETNDSELDTELLMKSFKASKRKSFHLGSPKPSPKTVQERLLTRTDEEQKLQYQIPTTCKDDELTSDLATICHNDLPPSSNASKLPLCEESSQRSQSSFGNAAVASLIQANASVATGDQKEAFLKNSGNSTCSGLSPNRVARSSQDSKLPTNSDSAASYQGFFPKVTSEESSICSSSEVQKNELQKSPKLGTCQPRIAEMSNKVEGSDVQTKHSSDSNKSPNIAAAGSPNLGAHSNQFESSITPDGLIPKGPVPYECQVTEPASNHSLNEENDAGSQPCVPRKRRAQRLESSESELSVEDDSLPTLAHIFKSHRLPSHSGQEPNQTSEISELDHQEPDPGYQSPADQEPDPGYQSPADQEPDPGFQSPADQANKSNEATPEAQSQNQECPENANATADDDHLPQPSCREEWVTSSQGSVDLFGTPEESEAVGICGNVAVSRDSSQYSSEIITTQQKEEMQQELCRLERMMALVSEALQKKEKDTSAKTQNKALNPTHSELQSAADPDQAEPCSPALPADRQSGLDSDGDHSGVSPGPAAPDGLCAPESQPLLMTDQAKTGRRVSRSRQKSQGRGRKSSQKAEAGLSLRNHNTTSEGSVTELTKGNEAKEAQKQTRGADMRRLGSSSTAGKMELVASGLSASELMLVKKFARKMHGHLSKEMTPGTTHVIIKTDEDLVCERTLKYFQGIAGRKWVVSFLWISECFNQGQVLDEAQFEVRGDVVNGHNHNGPLKSRTTSDGLLMKGYEICFQGSFTAMTTDQMEAMVEMCGATVVKDPLMFSKHIESKCQLVVVQPGSDDSQTYYRALQKKATVVSRSWLLDTISTYSLQNPQDHKP

Target ORF information:

RefSeq Version	XM_022669403.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus BRCA1, DNA repair associated (brca1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022669403.1

ORF Insert Sequence:

ATGACCGCTC CCAAAGCCGA GGACATCAAG CGGGGAATTG CTGTGATATG GGAGAATTTG 
CAGTGTCCAA TATGTTTGGA CTTGATGAAT GCACCAGTAT CGACTAGGTG TGATCACCAG 
TTCTGCAAGT TCTGCCTGAT GAAACTGCTG GAAAGGAGTA AAAAGAAAGA GACGAGCTGC 
CCTGTGTGCA AGATGAATGT CACTAAAAGG AGTCTGAAAG AAAGTCCAGG GTTCCAGAGG 
CTGGTGGAAG GACTGCAGAA TTTAGTCCAG TCTTATGAAT TTGACACCTG TACAAGCTAT 
TTTACAGGAA TACCACAGAG CAGGAAAGAA ACCAGTGTGG AAACAGAGTC CAGAGATCAG 
CAGTGCAGTG AGGAAAATGG CTCCTCAGGG AACGATGTGA CAGATAAGTC AGCATCTCAT 
TTCTCCTCGG CTGCAGTGAA GGATGCATTT GCAGAACTCA TGGGTCTTGT GGATTCCTGT 
ACAGTTAATT CAGAGCAGGA CTGTTTTGAC AGTGGTCTTG GCGGTCCTGT TAATAAAGCA 
GATGAGCTTC AAGATGTTCC TTCAAGAATA GAAGACCGAC AGAGTAATTC ACCCATACCT 
GATAGCAGCG GTTCTGATGG TAAGACTGAC CTACACAAGA GACGGTCAAG CAGAAGACCA 
AAAAGAGTAG GCTTGGAGCC AGACAGAATT GCAGACAAAA GACAAAAGAA AAGTGTGGAA 
AAAGTAGCAG AGTGGTTGCT AAAGATTTCA CCTTCATCAG ACACACAAAC CAGAGACAGG 
AGTAGAGATC CCTCAGTTCC CGCTGATAGT GATTCTGAAA AGGAGAGTGT GTGTTCATCC 
TCCCCTACAG AGGCTTATCC GAAAGATGGC GATGAGGATG ACGTCAATCC ACCAAAGGAG 
GTGCGTGGAC GTGGTCTAGA GGAAAAGGTT TTTGGGGCTG TCTATAAACG TGAACGTAAA 
GGTGGTAAAA CCAAAGTAAA CAGACCTGTC TCTCCTGAAA AAGAGACCGT GACCAACCCT 
GTTCCCTTTA ATTTGGTGCA AGAGGAAGAA ATGATGACAC GACATGGCCT TAAGAGAAAG 
AGGAGCTGTA GGCTGACTCC GGCAGATTTC ATTAAGAAAT CATCTTCTGA CGGTAGAGAA 
GCAGAAGTTA AAGAGCCCGT GGACTCTGTT ACTCATAATG CTGTGGAAGA AAACAAGCAA 
GTTGAATTAA ATACTGAGGA ACAAATAGAA ATTTTGGGAG AGTGTGACAA GATAGCAGAG 
CTCTCTGCAG ATGCAAAATG TGACAATACA GTTAAAAACA TGGAAGAAAG TCCTGCGATT 
GAAGTACCTT TGAAGAGATC AGGGAGAAGA TCAAAGTCAA AAATGCAGGA TATATGGCAA 
AATGTGGATC TTGACTTAAA AGAAAAAGAA AACATTTGTG ACAATAGAAC AAGTCGCAAG 
AGGCGGAGCA ATAGGAGCAG CTGTGAGCTG ACAAAAGATG GCCACTTGGA TGAAAGCAAG 
AATGCAAAGT GTACAAAATC CCTTACCCTT GTGGATGCGG CTGCTAATAA TGTTGATGTA 
ATGGAGCAGT TGAAAAATAA ACCAAAAATA GTGGAAGCAG AAATTAATAT TGAGAGTTAC 
CCAAGCAGTG CAGATCCACG ATCTCCTGAT GCTAGAAAAA CAAGAAGAAG TTTAAGGCTT 
CAGGCCTTCA CTACAGAAAT TCAGGGAACT CTGAAACAGA GACGAACTAC AAAACCTTCC 
AGTGGACCTG AAACTCATCC GTTAGATAAT CCATCACACT TTAATTGTTT GGACCAACCT 
AATGTGGATA AAGTGAATGC AAATGAAGAA AATGTTGATG CTGATCAGGG TATTGGAAGT 
ACTCAACAGT CTCGCAGTGG AAAAACTGAC CTGCTGATCA TGAAAAATGG ATGTGTATTC 
AACAGCAGCT TAGGAACAAT TGAGGCAGTG CAGACTAATG AGGAAACAGC TACTTCTCCA 
TGTGTTTCAG AGGGACAAGG CCCTGAACAA AATTCTGTCC TGTCCATTAT TCCAGACACA 
GTGGCTCAAG ATGGACCAAG CCTTCCTTGT TCTTCTGTGG CTGCCAGCTC TGCATTTCCA 
TTACAGGCTG TCACACTAGA CTCATCCTTA CCAGAGAAAA GCCAGACGTC TCCATCCGCA 
GGGTTCGTCG CCCCGTCTGT TGTACAGACA TTGAAAGATG CTCATTGTGA GCGTGGAGGC 
ATAGAAGTGG AAGGTGAGAC CAATGATAGT GAACTTGATA CAGAGCTTCT AATGAAGTCC 
TTCAAAGCTT CCAAAAGAAA ATCTTTCCAC CTGGGCAGCC CCAAGCCTTC ACCTAAAACA 
GTCCAGGAGA GGCTTCTAAC CAGGACTGAC GAGGAACAGA AGCTGCAATA TCAGATACCA 
ACCACTTGTA AAGACGATGA ACTAACATCA GATCTTGCAA CCATTTGCCA TAATGACTTG 
CCTCCATCTT CAAATGCTTC TAAGCTTCCG TTATGTGAGG AATCGTCTCA AAGGTCTCAG 
AGCAGTTTTG GTAATGCAGC TGTTGCAAGT CTTATCCAAG CAAATGCATC TGTTGCCACA 
GGAGATCAAA AGGAAGCTTT TCTGAAGAAC TCCGGCAACA GCACATGTTC AGGCCTAAGT 
CCCAACAGAG TTGCAAGATC TTCTCAAGAT TCCAAGCTTC CTACAAATTC TGACTCTGCA 
GCATCCTACC AGGGGTTCTT TCCTAAAGTT ACTTCAGAGG AGTCATCAAT CTGCAGCAGC 
AGCGAAGTCC AAAAGAATGA GCTCCAGAAA TCTCCAAAAT TAGGTACTTG TCAACCAAGG 
ATTGCCGAGA TGTCAAACAA AGTCGAGGGC AGCGATGTGC AGACAAAACA TTCTTCAGAC 
TCAAACAAAA GCCCAAACAT CGCAGCAGCA GGTTCACCAA ATCTTGGAGC TCACAGTAAT 
CAATTTGAAT CCTCAATCAC CCCAGATGGA CTCATACCAA AAGGTCCTGT TCCTTATGAA 
TGTCAAGTCA CAGAACCTGC ATCTAATCAC AGTTTGAATG AGGAAAATGA TGCAGGGTCT 
CAGCCGTGCG TGCCGAGAAA GAGGAGGGCT CAGAGGCTCG AGTCCTCAGA GTCTGAGCTC 
AGTGTTGAAG ATGATTCTCT TCCAACACTG GCTCATATAT TCAAATCTCA CCGTTTACCC 
TCACACTCTG GGCAGGAACC AAATCAGACC TCTGAGATTT CTGAGTTAGA TCACCAAGAA 
CCAGATCCAG GCTATCAATC TCCAGCTGAC CAAGAACCAG ATCCAGGCTA TCAATCTCCA 
GCTGACCAAG AACCAGATCC AGGCTTTCAA TCTCCAGCTG ACCAGGCTAA CAAATCAAAT 
GAAGCAACAC CAGAGGCACA GTCTCAGAAT CAGGAATGTC CTGAAAATGC AAATGCTACA 
GCAGACGATG ACCATCTACC TCAACCTTCC TGCAGAGAGG AATGGGTTAC ATCGAGCCAA 
GGATCTGTGG ATCTCTTCGG GACGCCAGAA GAAAGTGAAG CCGTTGGTAT CTGTGGAAAT 
GTTGCAGTTT CCAGGGATTC CTCTCAGTAC TCGAGTGAGA TTATCACCAC ACAGCAGAAA 
GAGGAGATGC AGCAGGAGCT GTGTAGGCTG GAGAGGATGA TGGCTCTGGT GTCTGAGGCT 
CTCCAGAAGA AGGAGAAGGA CACAAGCGCC AAAACCCAGA ACAAGGCTCT CAACCCTACT 
CACTCTGAAC TACAGTCAGC TGCTGACCCT GACCAGGCTG AGCCGTGCAG CCCTGCACTC 
CCTGCAGACA GGCAGAGCGG CCTTGATTCT GACGGAGATC ACAGTGGGGT GTCCCCTGGC 
CCTGCAGCTC CAGACGGCCT GTGTGCTCCC GAGTCCCAAC CTCTGTTGAT GACAGACCAG 
GCAAAGACAG GGCGGCGGGT CAGCAGGAGC CGTCAGAAAT CTCAGGGCAG GGGGAGGAAG 
TCTTCACAGA AGGCAGAAGC TGGGTTATCT CTCAGAAATC ACAACACCAC CTCGGAGGGT 
TCGGTTACGG AGCTGACTAA GGGAAACGAG GCAAAAGAAG CCCAGAAACA AACCCGTGGA 
GCGGACATGC GCAGACTGGG GTCCAGCAGC ACAGCAGGGA AGATGGAGCT GGTGGCTTCT 
GGGCTCAGTG CCAGCGAATT GATGTTGGTG AAGAAATTTG CCAGGAAAAT GCACGGCCAT 
CTAAGTAAAG AGATGACCCC CGGTACTACC CATGTCATCA TTAAGACAGA TGAGGACCTC 
GTGTGTGAGC GCACCTTAAA GTACTTTCAG GGGATCGCCG GCAGGAAGTG GGTGGTGAGC 
TTCCTGTGGA TCTCGGAGTG CTTTAATCAA GGCCAAGTTC TAGACGAGGC TCAGTTTGAA 
GTTCGTGGAG ATGTTGTTAA TGGACACAAC CACAACGGGC CCTTAAAAAG CCGGACCACC 
TCTGATGGGT TGCTGATGAA AGGATACGAG ATCTGCTTCC AGGGATCCTT CACAGCGATG 
ACTACAGATC AGATGGAGGC TATGGTGGAG ATGTGTGGCG CTACTGTTGT AAAAGACCCG 
CTCATGTTCT CCAAACACAT AGAAAGTAAG TGTCAGCTGG TGGTGGTGCA GCCTGGTTCT 
GATGACTCTC AAACTTACTA CAGAGCTCTG CAGAAGAAAG CCACAGTGGT GTCTCGTAGC 
TGGCTCTTGG ATACCATATC AACCTACAGC CTCCAGAATC CCCAGGACCA TAAACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brca1 ( XM_022669404.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_022525125.1 Astyanax mexicanus BRCA1, DNA repair associated (brca1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAs125387
Clone ID Related Accession (Same CDS sequence)	XM_022669404.1
Accession Version	XM_022669404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	breast cancer type 1 susceptibility protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCTC CCAAAGCCGA GGACATCAAG CGGGGAATTG CTGTGATATG GGAGAATTTG 
CAGTGTCCAA TATGTTTGGA CTTGATGAAT GCACCAGTAT CGACTAGGTG TGATCACCAG 
TTCTGCAAGT TCTGCCTGAT GAAACTGCTG GAAAGGAGTA AAAAGAAAGA GACGAGCTGC 
CCTGTGTGCA AGATGAATGT CACTAAAAGG AGTCTGAAAG AAAGTCCAGG GTTCCAGAGG 
CTGGTGGAAG GACTGCAGAA TTTAGTCCAG TCTTATGAAT TTGACACCTG TACAAGCTAT 
TTTACAGGAA TACCACAGAG CAGGAAAGAA ACCAGTGTGG AAACAGAGTC CAGAGATCAG 
CAGTGCAGTG AGGAAAATGG CTCCTCAGGG AACGATGTGA CAGATAAGTC AGCATCTCAT 
TTCTCCTCGG CTGCAGTGAA GGATGCATTT GCAGAACTCA TGGGTCTTGT GGATTCCTGT 
ACAGTTAATT CAGAGCAGGA CTGTTTTGAC AGTGGTCTTG GCGGTCCTGT TAATAAAGCA 
GATGAGCTTC AAGATGTTCC TTCAAGAATA GAAGACCGAC AGAGTAATTC ACCCATACCT 
GATAGCAGCG GTTCTGATGG TAAGACTGAC CTACACAAGA GACGGTCAAG CAGAAGACCA 
AAAAGAGTAG GCTTGGAGCC AGACAGAATT GCAGACAAAA GACAAAAGAA AAGTGTGGAA 
AAAGTAGCAG AGTGGTTGCT AAAGATTTCA CCTTCATCAG ACACACAAAC CAGAGACAGG 
AGTAGAGATC CCTCAGTTCC CGCTGATAGT GATTCTGAAA AGGAGAGTGT GTGTTCATCC 
TCCCCTACAG AGGCTTATCC GAAAGATGGC GATGAGGATG ACGTCAATCC ACCAAAGGAG 
GTGCGTGGAC GTGGTCTAGA GGAAAAGGTT TTTGGGGCTG TCTATAAACG TGAACGTAAA 
GGTGGTAAAA CCAAAGTAAA CAGACCTGTC TCTCCTGAAA AAGAGACCGT GACCAACCCT 
GTTCCCTTTA ATTTGGTGCA AGAGGAAGAA ATGATGACAC GACATGGCCT TAAGAGAAAG 
AGGAGCTGTA GGCTGACTCC GGCAGATTTC ATTAAGAAAT CATCTTCTGA CGGTAGAGAA 
GCAGAAGTTA AAGAGCCCGT GGACTCTGTT ACTCATAATG CTGTGGAAGA AAACAAGCAA 
GTTGAATTAA ATACTGAGGA ACAAATAGAA ATTTTGGGAG AGTGTGACAA GATAGCAGAG 
CTCTCTGCAG ATGCAAAATG TGACAATACA GTTAAAAACA TGGAAGAAAG TCCTGCGATT 
GAAGTACCTT TGAAGAGATC AGGGAGAAGA TCAAAGTCAA AAATGCAGGA TATATGGCAA 
AATGTGGATC TTGACTTAAA AGAAAAAGAA AACATTTGTG ACAATAGAAC AAGTCGCAAG 
AGGCGGAGCA ATAGGAGCAG CTGTGAGCTG ACAAAAGATG GCCACTTGGA TGAAAGCAAG 
AATGCAAAGT GTACAAAATC CCTTACCCTT GTGGATGCGG CTGCTAATAA TGTTGATGTA 
ATGGAGCAGT TGAAAAATAA ACCAAAAATA GTGGAAGCAG AAATTAATAT TGAGAGTTAC 
CCAAGCAGTG CAGATCCACG ATCTCCTGAT GCTAGAAAAA CAAGAAGAAG TTTAAGGCTT 
CAGGCCTTCA CTACAGAAAT TCAGGGAACT CTGAAACAGA GACGAACTAC AAAACCTTCC 
AGTGGACCTG AAACTCATCC GTTAGATAAT CCATCACACT TTAATTGTTT GGACCAACCT 
AATGTGGATA AAGTGAATGC AAATGAAGAA AATGTTGATG CTGATCAGGG TATTGGAAGT 
ACTCAACAGT CTCGCAGTGG AAAAACTGAC CTGCTGATCA TGAAAAATGG ATGTGTATTC 
AACAGCAGCT TAGGAACAAT TGAGGCAGTG CAGACTAATG AGGAAACAGC TACTTCTCCA 
TGTGTTTCAG AGGGACAAGG CCCTGAACAA AATTCTGTCC TGTCCATTAT TCCAGACACA 
GTGGCTCAAG ATGGACCAAG CCTTCCTTGT TCTTCTGTGG CTGCCAGCTC TGCATTTCCA 
TTACAGGCTG TCACACTAGA CTCATCCTTA CCAGAGAAAA GCCAGACGTC TCCATCCGCA 
GGGTTCGTCG CCCCGTCTGT TGTACAGACA TTGAAAGATG CTCATTGTGA GCGTGGAGGC 
ATAGAAGTGG AAGGTGAGAC CAATGATAGT GAACTTGATA CAGAGCTTCT AATGAAGTCC 
TTCAAAGCTT CCAAAAGAAA ATCTTTCCAC CTGGGCAGCC CCAAGCCTTC ACCTAAAACA 
GTCCAGGAGA GGCTTCTAAC CAGGACTGAC GAGGAACAGA AGCTGCAATA TCAGATACCA 
ACCACTTGTA AAGACGATGA ACTAACATCA GATCTTGCAA CCATTTGCCA TAATGACTTG 
CCTCCATCTT CAAATGCTTC TAAGCTTCCG TTATGTGAGG AATCGTCTCA AAGGTCTCAG 
AGCAGTTTTG GTAATGCAGC TGTTGCAAGT CTTATCCAAG CAAATGCATC TGTTGCCACA 
GGAGATCAAA AGGAAGCTTT TCTGAAGAAC TCCGGCAACA GCACATGTTC AGGCCTAAGT 
CCCAACAGAG TTGCAAGATC TTCTCAAGAT TCCAAGCTTC CTACAAATTC TGACTCTGCA 
GCATCCTACC AGGGGTTCTT TCCTAAAGTT ACTTCAGAGG AGTCATCAAT CTGCAGCAGC 
AGCGAAGTCC AAAAGAATGA GCTCCAGAAA TCTCCAAAAT TAGGTACTTG TCAACCAAGG 
ATTGCCGAGA TGTCAAACAA AGTCGAGGGC AGCGATGTGC AGACAAAACA TTCTTCAGAC 
TCAAACAAAA GCCCAAACAT CGCAGCAGCA GGTTCACCAA ATCTTGGAGC TCACAGTAAT 
CAATTTGAAT CCTCAATCAC CCCAGATGGA CTCATACCAA AAGGTCCTGT TCCTTATGAA 
TGTCAAGTCA CAGAACCTGC ATCTAATCAC AGTTTGAATG AGGAAAATGA TGCAGGGTCT 
CAGCCGTGCG TGCCGAGAAA GAGGAGGGCT CAGAGGCTCG AGTCCTCAGA GTCTGAGCTC 
AGTGTTGAAG ATGATTCTCT TCCAACACTG GCTCATATAT TCAAATCTCA CCGTTTACCC 
TCACACTCTG GGCAGGAACC AAATCAGACC TCTGAGATTT CTGAGTTAGA TCACCAAGAA 
CCAGATCCAG GCTATCAATC TCCAGCTGAC CAAGAACCAG ATCCAGGCTA TCAATCTCCA 
GCTGACCAAG AACCAGATCC AGGCTTTCAA TCTCCAGCTG ACCAGGCTAA CAAATCAAAT 
GAAGCAACAC CAGAGGCACA GTCTCAGAAT CAGGAATGTC CTGAAAATGC AAATGCTACA 
GCAGACGATG ACCATCTACC TCAACCTTCC TGCAGAGAGG AATGGGTTAC ATCGAGCCAA 
GGATCTGTGG ATCTCTTCGG GACGCCAGAA GAAAGTGAAG CCGTTGGTAT CTGTGGAAAT 
GTTGCAGTTT CCAGGGATTC CTCTCAGTAC TCGAGTGAGA TTATCACCAC ACAGAAAGAG 
GAGATGCAGC AGGAGCTGTG TAGGCTGGAG AGGATGATGG CTCTGGTGTC TGAGGCTCTC 
CAGAAGAAGG AGAAGGACAC AAGCGCCAAA ACCCAGAACA AGGCTCTCAA CCCTACTCAC 
TCTGAACTAC AGTCAGCTGC TGACCCTGAC CAGGCTGAGC CGTGCAGCCC TGCACTCCCT 
GCAGACAGGC AGAGCGGCCT TGATTCTGAC GGAGATCACA GTGGGGTGTC CCCTGGCCCT 
GCAGCTCCAG ACGGCCTGTG TGCTCCCGAG TCCCAACCTC TGTTGATGAC AGACCAGGCA 
AAGACAGGGC GGCGGGTCAG CAGGAGCCGT CAGAAATCTC AGGGCAGGGG GAGGAAGTCT 
TCACAGAAGG CAGAAGCTGG GTTATCTCTC AGAAATCACA ACACCACCTC GGAGGGTTCG 
GTTACGGAGC TGACTAAGGG AAACGAGGCA AAAGAAGCCC AGAAACAAAC CCGTGGAGCG 
GACATGCGCA GACTGGGGTC CAGCAGCACA GCAGGGAAGA TGGAGCTGGT GGCTTCTGGG 
CTCAGTGCCA GCGAATTGAT GTTGGTGAAG AAATTTGCCA GGAAAATGCA CGGCCATCTA 
AGTAAAGAGA TGACCCCCGG TACTACCCAT GTCATCATTA AGACAGATGA GGACCTCGTG 
TGTGAGCGCA CCTTAAAGTA CTTTCAGGGG ATCGCCGGCA GGAAGTGGGT GGTGAGCTTC 
CTGTGGATCT CGGAGTGCTT TAATCAAGGC CAAGTTCTAG ACGAGGCTCA GTTTGAAGTT 
CGTGGAGATG TTGTTAATGG ACACAACCAC AACGGGCCCT TAAAAAGCCG GACCACCTCT 
GATGGGTTGC TGATGAAAGG ATACGAGATC TGCTTCCAGG GATCCTTCAC AGCGATGACT 
ACAGATCAGA TGGAGGCTAT GGTGGAGATG TGTGGCGCTA CTGTTGTAAA AGACCCGCTC 
ATGTTCTCCA AACACATAGA AAGTAAGTGT CAGCTGGTGG TGGTGCAGCC TGGTTCTGAT 
GACTCTCAAA CTTACTACAG AGCTCTGCAG AAGAAAGCCA CAGTGGTGTC TCGTAGCTGG 
CTCTTGGATA CCATATCAAC CTACAGCCTC CAGAATCCCC AGGACCATAA ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022525125.1
CDS	176..4912
Translation	MTAPKAEDIKRGIAVIWENLQCPICLDLMNAPVSTRCDHQFCKFCLMKLLERSKKKETSCPVCKMNVTKRSLKESPGFQRLVEGLQNLVQSYEFDTCTSYFTGIPQSRKETSVETESRDQQCSEENGSSGNDVTDKSASHFSSAAVKDAFAELMGLVDSCTVNSEQDCFDSGLGGPVNKADELQDVPSRIEDRQSNSPIPDSSGSDGKTDLHKRRSSRRPKRVGLEPDRIADKRQKKSVEKVAEWLLKISPSSDTQTRDRSRDPSVPADSDSEKESVCSSSPTEAYPKDGDEDDVNPPKEVRGRGLEEKVFGAVYKRERKGGKTKVNRPVSPEKETVTNPVPFNLVQEEEMMTRHGLKRKRSCRLTPADFIKKSSSDGREAEVKEPVDSVTHNAVEENKQVELNTEEQIEILGECDKIAELSADAKCDNTVKNMEESPAIEVPLKRSGRRSKSKMQDIWQNVDLDLKEKENICDNRTSRKRRSNRSSCELTKDGHLDESKNAKCTKSLTLVDAAANNVDVMEQLKNKPKIVEAEINIESYPSSADPRSPDARKTRRSLRLQAFTTEIQGTLKQRRTTKPSSGPETHPLDNPSHFNCLDQPNVDKVNANEENVDADQGIGSTQQSRSGKTDLLIMKNGCVFNSSLGTIEAVQTNEETATSPCVSEGQGPEQNSVLSIIPDTVAQDGPSLPCSSVAASSAFPLQAVTLDSSLPEKSQTSPSAGFVAPSVVQTLKDAHCERGGIEVEGETNDSELDTELLMKSFKASKRKSFHLGSPKPSPKTVQERLLTRTDEEQKLQYQIPTTCKDDELTSDLATICHNDLPPSSNASKLPLCEESSQRSQSSFGNAAVASLIQANASVATGDQKEAFLKNSGNSTCSGLSPNRVARSSQDSKLPTNSDSAASYQGFFPKVTSEESSICSSSEVQKNELQKSPKLGTCQPRIAEMSNKVEGSDVQTKHSSDSNKSPNIAAAGSPNLGAHSNQFESSITPDGLIPKGPVPYECQVTEPASNHSLNEENDAGSQPCVPRKRRAQRLESSESELSVEDDSLPTLAHIFKSHRLPSHSGQEPNQTSEISELDHQEPDPGYQSPADQEPDPGYQSPADQEPDPGFQSPADQANKSNEATPEAQSQNQECPENANATADDDHLPQPSCREEWVTSSQGSVDLFGTPEESEAVGICGNVAVSRDSSQYSSEIITTQKEEMQQELCRLERMMALVSEALQKKEKDTSAKTQNKALNPTHSELQSAADPDQAEPCSPALPADRQSGLDSDGDHSGVSPGPAAPDGLCAPESQPLLMTDQAKTGRRVSRSRQKSQGRGRKSSQKAEAGLSLRNHNTTSEGSVTELTKGNEAKEAQKQTRGADMRRLGSSSTAGKMELVASGLSASELMLVKKFARKMHGHLSKEMTPGTTHVIIKTDEDLVCERTLKYFQGIAGRKWVVSFLWISECFNQGQVLDEAQFEVRGDVVNGHNHNGPLKSRTTSDGLLMKGYEICFQGSFTAMTTDQMEAMVEMCGATVVKDPLMFSKHIESKCQLVVVQPGSDDSQTYYRALQKKATVVSRSWLLDTISTYSLQNPQDHKP

Target ORF information:

RefSeq Version	XM_022669404.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus BRCA1, DNA repair associated (brca1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022669404.1

ORF Insert Sequence:

ATGACCGCTC CCAAAGCCGA GGACATCAAG CGGGGAATTG CTGTGATATG GGAGAATTTG 
CAGTGTCCAA TATGTTTGGA CTTGATGAAT GCACCAGTAT CGACTAGGTG TGATCACCAG 
TTCTGCAAGT TCTGCCTGAT GAAACTGCTG GAAAGGAGTA AAAAGAAAGA GACGAGCTGC 
CCTGTGTGCA AGATGAATGT CACTAAAAGG AGTCTGAAAG AAAGTCCAGG GTTCCAGAGG 
CTGGTGGAAG GACTGCAGAA TTTAGTCCAG TCTTATGAAT TTGACACCTG TACAAGCTAT 
TTTACAGGAA TACCACAGAG CAGGAAAGAA ACCAGTGTGG AAACAGAGTC CAGAGATCAG 
CAGTGCAGTG AGGAAAATGG CTCCTCAGGG AACGATGTGA CAGATAAGTC AGCATCTCAT 
TTCTCCTCGG CTGCAGTGAA GGATGCATTT GCAGAACTCA TGGGTCTTGT GGATTCCTGT 
ACAGTTAATT CAGAGCAGGA CTGTTTTGAC AGTGGTCTTG GCGGTCCTGT TAATAAAGCA 
GATGAGCTTC AAGATGTTCC TTCAAGAATA GAAGACCGAC AGAGTAATTC ACCCATACCT 
GATAGCAGCG GTTCTGATGG TAAGACTGAC CTACACAAGA GACGGTCAAG CAGAAGACCA 
AAAAGAGTAG GCTTGGAGCC AGACAGAATT GCAGACAAAA GACAAAAGAA AAGTGTGGAA 
AAAGTAGCAG AGTGGTTGCT AAAGATTTCA CCTTCATCAG ACACACAAAC CAGAGACAGG 
AGTAGAGATC CCTCAGTTCC CGCTGATAGT GATTCTGAAA AGGAGAGTGT GTGTTCATCC 
TCCCCTACAG AGGCTTATCC GAAAGATGGC GATGAGGATG ACGTCAATCC ACCAAAGGAG 
GTGCGTGGAC GTGGTCTAGA GGAAAAGGTT TTTGGGGCTG TCTATAAACG TGAACGTAAA 
GGTGGTAAAA CCAAAGTAAA CAGACCTGTC TCTCCTGAAA AAGAGACCGT GACCAACCCT 
GTTCCCTTTA ATTTGGTGCA AGAGGAAGAA ATGATGACAC GACATGGCCT TAAGAGAAAG 
AGGAGCTGTA GGCTGACTCC GGCAGATTTC ATTAAGAAAT CATCTTCTGA CGGTAGAGAA 
GCAGAAGTTA AAGAGCCCGT GGACTCTGTT ACTCATAATG CTGTGGAAGA AAACAAGCAA 
GTTGAATTAA ATACTGAGGA ACAAATAGAA ATTTTGGGAG AGTGTGACAA GATAGCAGAG 
CTCTCTGCAG ATGCAAAATG TGACAATACA GTTAAAAACA TGGAAGAAAG TCCTGCGATT 
GAAGTACCTT TGAAGAGATC AGGGAGAAGA TCAAAGTCAA AAATGCAGGA TATATGGCAA 
AATGTGGATC TTGACTTAAA AGAAAAAGAA AACATTTGTG ACAATAGAAC AAGTCGCAAG 
AGGCGGAGCA ATAGGAGCAG CTGTGAGCTG ACAAAAGATG GCCACTTGGA TGAAAGCAAG 
AATGCAAAGT GTACAAAATC CCTTACCCTT GTGGATGCGG CTGCTAATAA TGTTGATGTA 
ATGGAGCAGT TGAAAAATAA ACCAAAAATA GTGGAAGCAG AAATTAATAT TGAGAGTTAC 
CCAAGCAGTG CAGATCCACG ATCTCCTGAT GCTAGAAAAA CAAGAAGAAG TTTAAGGCTT 
CAGGCCTTCA CTACAGAAAT TCAGGGAACT CTGAAACAGA GACGAACTAC AAAACCTTCC 
AGTGGACCTG AAACTCATCC GTTAGATAAT CCATCACACT TTAATTGTTT GGACCAACCT 
AATGTGGATA AAGTGAATGC AAATGAAGAA AATGTTGATG CTGATCAGGG TATTGGAAGT 
ACTCAACAGT CTCGCAGTGG AAAAACTGAC CTGCTGATCA TGAAAAATGG ATGTGTATTC 
AACAGCAGCT TAGGAACAAT TGAGGCAGTG CAGACTAATG AGGAAACAGC TACTTCTCCA 
TGTGTTTCAG AGGGACAAGG CCCTGAACAA AATTCTGTCC TGTCCATTAT TCCAGACACA 
GTGGCTCAAG ATGGACCAAG CCTTCCTTGT TCTTCTGTGG CTGCCAGCTC TGCATTTCCA 
TTACAGGCTG TCACACTAGA CTCATCCTTA CCAGAGAAAA GCCAGACGTC TCCATCCGCA 
GGGTTCGTCG CCCCGTCTGT TGTACAGACA TTGAAAGATG CTCATTGTGA GCGTGGAGGC 
ATAGAAGTGG AAGGTGAGAC CAATGATAGT GAACTTGATA CAGAGCTTCT AATGAAGTCC 
TTCAAAGCTT CCAAAAGAAA ATCTTTCCAC CTGGGCAGCC CCAAGCCTTC ACCTAAAACA 
GTCCAGGAGA GGCTTCTAAC CAGGACTGAC GAGGAACAGA AGCTGCAATA TCAGATACCA 
ACCACTTGTA AAGACGATGA ACTAACATCA GATCTTGCAA CCATTTGCCA TAATGACTTG 
CCTCCATCTT CAAATGCTTC TAAGCTTCCG TTATGTGAGG AATCGTCTCA AAGGTCTCAG 
AGCAGTTTTG GTAATGCAGC TGTTGCAAGT CTTATCCAAG CAAATGCATC TGTTGCCACA 
GGAGATCAAA AGGAAGCTTT TCTGAAGAAC TCCGGCAACA GCACATGTTC AGGCCTAAGT 
CCCAACAGAG TTGCAAGATC TTCTCAAGAT TCCAAGCTTC CTACAAATTC TGACTCTGCA 
GCATCCTACC AGGGGTTCTT TCCTAAAGTT ACTTCAGAGG AGTCATCAAT CTGCAGCAGC 
AGCGAAGTCC AAAAGAATGA GCTCCAGAAA TCTCCAAAAT TAGGTACTTG TCAACCAAGG 
ATTGCCGAGA TGTCAAACAA AGTCGAGGGC AGCGATGTGC AGACAAAACA TTCTTCAGAC 
TCAAACAAAA GCCCAAACAT CGCAGCAGCA GGTTCACCAA ATCTTGGAGC TCACAGTAAT 
CAATTTGAAT CCTCAATCAC CCCAGATGGA CTCATACCAA AAGGTCCTGT TCCTTATGAA 
TGTCAAGTCA CAGAACCTGC ATCTAATCAC AGTTTGAATG AGGAAAATGA TGCAGGGTCT 
CAGCCGTGCG TGCCGAGAAA GAGGAGGGCT CAGAGGCTCG AGTCCTCAGA GTCTGAGCTC 
AGTGTTGAAG ATGATTCTCT TCCAACACTG GCTCATATAT TCAAATCTCA CCGTTTACCC 
TCACACTCTG GGCAGGAACC AAATCAGACC TCTGAGATTT CTGAGTTAGA TCACCAAGAA 
CCAGATCCAG GCTATCAATC TCCAGCTGAC CAAGAACCAG ATCCAGGCTA TCAATCTCCA 
GCTGACCAAG AACCAGATCC AGGCTTTCAA TCTCCAGCTG ACCAGGCTAA CAAATCAAAT 
GAAGCAACAC CAGAGGCACA GTCTCAGAAT CAGGAATGTC CTGAAAATGC AAATGCTACA 
GCAGACGATG ACCATCTACC TCAACCTTCC TGCAGAGAGG AATGGGTTAC ATCGAGCCAA 
GGATCTGTGG ATCTCTTCGG GACGCCAGAA GAAAGTGAAG CCGTTGGTAT CTGTGGAAAT 
GTTGCAGTTT CCAGGGATTC CTCTCAGTAC TCGAGTGAGA TTATCACCAC ACAGAAAGAG 
GAGATGCAGC AGGAGCTGTG TAGGCTGGAG AGGATGATGG CTCTGGTGTC TGAGGCTCTC 
CAGAAGAAGG AGAAGGACAC AAGCGCCAAA ACCCAGAACA AGGCTCTCAA CCCTACTCAC 
TCTGAACTAC AGTCAGCTGC TGACCCTGAC CAGGCTGAGC CGTGCAGCCC TGCACTCCCT 
GCAGACAGGC AGAGCGGCCT TGATTCTGAC GGAGATCACA GTGGGGTGTC CCCTGGCCCT 
GCAGCTCCAG ACGGCCTGTG TGCTCCCGAG TCCCAACCTC TGTTGATGAC AGACCAGGCA 
AAGACAGGGC GGCGGGTCAG CAGGAGCCGT CAGAAATCTC AGGGCAGGGG GAGGAAGTCT 
TCACAGAAGG CAGAAGCTGG GTTATCTCTC AGAAATCACA ACACCACCTC GGAGGGTTCG 
GTTACGGAGC TGACTAAGGG AAACGAGGCA AAAGAAGCCC AGAAACAAAC CCGTGGAGCG 
GACATGCGCA GACTGGGGTC CAGCAGCACA GCAGGGAAGA TGGAGCTGGT GGCTTCTGGG 
CTCAGTGCCA GCGAATTGAT GTTGGTGAAG AAATTTGCCA GGAAAATGCA CGGCCATCTA 
AGTAAAGAGA TGACCCCCGG TACTACCCAT GTCATCATTA AGACAGATGA GGACCTCGTG 
TGTGAGCGCA CCTTAAAGTA CTTTCAGGGG ATCGCCGGCA GGAAGTGGGT GGTGAGCTTC 
CTGTGGATCT CGGAGTGCTT TAATCAAGGC CAAGTTCTAG ACGAGGCTCA GTTTGAAGTT 
CGTGGAGATG TTGTTAATGG ACACAACCAC AACGGGCCCT TAAAAAGCCG GACCACCTCT 
GATGGGTTGC TGATGAAAGG ATACGAGATC TGCTTCCAGG GATCCTTCAC AGCGATGACT 
ACAGATCAGA TGGAGGCTAT GGTGGAGATG TGTGGCGCTA CTGTTGTAAA AGACCCGCTC 
ATGTTCTCCA AACACATAGA AAGTAAGTGT CAGCTGGTGG TGGTGCAGCC TGGTTCTGAT 
GACTCTCAAA CTTACTACAG AGCTCTGCAG AAGAAAGCCA CAGTGGTGTC TCGTAGCTGG 
CTCTTGGATA CCATATCAAC CTACAGCCTC CAGAATCCCC AGGACCATAA ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brca1 ( XM_022669405.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_022525126.1 Astyanax mexicanus BRCA1, DNA repair associated (brca1), transcript variant X3, mRNA.

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CloneID	OAs125388
Clone ID Related Accession (Same CDS sequence)	XM_022669405.1
Accession Version	XM_022669405.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	breast cancer type 1 susceptibility protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCTC CCAAAGCCGA GGACATCAAG CGGGGAATTG CTGTGATATG GGAGAATTTG 
CAGTGTCCAA TATGTTTGGA CTTGATGAAT GCACCAGTAT CGACTAGGTG TGATCACCAG 
TTCTGCAAGT TCTGCCTGAT GAAACTGCTG GAAAGGAGTA AAAAGAAAGA GACGAGCTGC 
CCTGTGTGCA AGATGAATGT CACTAAAAGG AGTCTGAAAG AAAGTCCAGG GTTCCAGAGG 
CTGGTGGAAG GACTGCAGAA TTTAGTCCAG TCTTATGAAT TTGACACCTG TACAAGCTAT 
TTTACAGGAA TACCACAGAG CAGGAAAGAA ACCAGTGTGG AAACAGAGTC CAGAGATCAG 
CAGTGCAGTG AGGAAAATGG CTCCTCAGGG AACGATGTGA CAGATAAGTC AGCATCTCAT 
TTCTCCTCGG CTGCAGTGAA GGATGCATTT GCAGAACTCA TGGGTCTTGT GGATTCCTGT 
ACAGTTAATT CAGAGCAGGA CTGTTTTGAC AGTGGTCTTG GCGGTCCTGT TAATAAAGCA 
GATGAGCTTC AAGATGTTCC TTCAAGAATA GAAGACCGAC AGAGTAATTC ACCCATACCT 
GATAGCAGCG GTTCTGATGG TAAGACTGAC CTACACAAGA GACGGTCAAG CAGAAGACCA 
AAAAGAGTAG GCTTGGAGCC AGACAGAATT GCAGACAAAA GACAAAAGAA AAGTGTGGAA 
AAAGTAGCAG AGTGGTTGCT AAAGATTTCA CCTTCATCAG ACACACAAAC CAGAGACAGG 
AGTAGAGATC CCTCAGTTCC CGCTGATAGT GATTCTGAAA AGGAGAGTGT GTGTTCATCC 
TCCCCTACAG AGGCTTATCC GAAAGATGGC GATGAGGATG ACGTCAATCC ACCAAAGGAG 
GTGCGTGGAC GTGGTCTAGA GGAAAAGGTT TTTGGGGCTG TCTATAAACG TGAACGTAAA 
GGTGGTAAAA CCAAAGTAAA CAGACCTGTC TCTCCTGAAA AAGAGACCGT GACCAACCCT 
GTTCCCTTTA ATTTGGTGCA AGAGGAAGAA ATGATGACAC GACATGGCCT TAAGAGAAAG 
AGGAGCTGTA GGCTGACTCC GGCAGATTTC ATTAAGAAAT CATCTTCTGA CGGTAGAGAA 
GCAGAAGTTA AAGAGCCCGT GGACTCTGTT ACTCATAATG CTGTGGAAGA AAACAAGCAA 
GTTGAATTAA ATACTGAGGA ACAAATAGAA ATTTTGGGAG AGTGTGACAA GATAGCAGAG 
CTCTCTGCAG ATGCAAAATG TGACAATACA GTTAAAAACA TGGAAGAAAG TCCTGCGATT 
GAAGTACCTT TGAAGAGATC AGGGAGAAGA TCAAAGTCAA AAATGCAGGA TATATGGCAA 
AATGTGGATC TTGACTTAAA AGAAAAAGAA AACATTTGTG ACAATAGAAC AAGTCGCAAG 
AGGCGGAGCA ATAGGAGCAG CTGTGAGCTG ACAAAAGATG GCCACTTGGA TGAAAGCAAG 
AATGCAAAGT GTACAAAATC CCTTACCCTT GTGGATGCGG CTGCTAATAA TGTTGATGTA 
ATGGAGCAGT TGAAAAATAA ACCAAAAATA GTGGAAGCAG AAATTAATAT TGAGAGTTAC 
CCAAGCAGTG CAGATCCACG ATCTCCTGAT GCTAGAAAAA CAAGAAGAAG TTTAAGGCTT 
CAGGCCTTCA CTACAGAAAT TCAGGGAACT CTGAAACAGA GACGAACTAC AAAACCTTCC 
AGTGGACCTG AAACTCATCC GTTAGATAAT CCATCACACT TTAATTGTTT GGACCAACCT 
AATGTGGATA AAGTGAATGC AAATGAAGAA AATGTTGATG CTGATCAGGG TATTGGAAGT 
ACTCAACAGT CTCGCAGTGG AAAAACTGAC CTGCTGATCA TGAAAAATGG ATGTGTATTC 
AACAGCAGCT TAGGAACAAT TGAGGCAGTG CAGACTAATG AGGAAACAGC TACTTCTCCA 
TGTGTTTCAG AGGGACAAGG CCCTGAACAA AATTCTGTCC TGTCCATTAT TCCAGACACA 
GTGGCTCAAG ATGGACCAAG CCTTCCTTGT TCTTCTGTGG CTGCCAGCTC TGCATTTCCA 
TTACAGGCTG TCACACTAGA CTCATCCTTA CCAGAGAAAA GCCAGACGTC TCCATCCGCA 
GGGTTCGTCG CCCCGTCTGT TGTACAGACA TTGAAAGATG CTCATTGTGA GCGTGGAGGC 
ATAGAAGTGG AAGGTGAGAC CAATGATAGT GAACTTGATA CAGAGCTTCT AATGAAGTCC 
TTCAAAGCTT CCAAAAGAAA ATCTTTCCAC CTGGGCAGCC CCAAGCCTTC ACCTAAAACA 
GTCCAGGAGA GGCTTCTAAC CAGGACTGAC GAGGAACAGA AGCTGCAATA TCAGATACCA 
ACCACTTGTA AAGACGATGA ACTAACATCA GATCTTGCAA CCATTTGCCA TAATGACTTG 
CCTCCATCTT CAAATGCTTC TAAGCTTCCG TTATGTGAGG AATCGTCTCA AAGGTCTCAG 
AGCAGTTTTG GTAATGCAGC TGTTGCAAGT CTTATCCAAG CAAATGCATC TGTTGCCACA 
GGAGATCAAA AGGAAGCTTT TCTGAAGAAC TCCGGCAACA GCACATGTTC AGGCCTAAGT 
CCCAACAGAG TTGCAAGATC TTCTCAAGAT TCCAAGCTTC CTACAAATTC TGACTCTGCA 
GCATCCTACC AGGGGTTCTT TCCTAAAGTT ACTTCAGAGG AGTCATCAAT CTGCAGCAGC 
AGCGAAGTCC AAAAGAATGA GCTCCAGAAA TCTCCAAAAT TAGGTACTTG TCAACCAAGG 
ATTGCCGAGA TGTCAAACAA AGTCGAGGGC AGCGATGTGC AGACAAAACA TTCTTCAGAC 
TCAAACAAAA GCCCAAACAT CGCAGCAGCA GGTTCACCAA ATCTTGGAGC TCACAGTAAT 
CAATTTGAAT CCTCAATCAC CCCAGATGGA CTCATACCAA AAGGTCCTGT TCCTTATGAA 
TGTCAAGTCA CAGAACCTGC ATCTAATCAC AGTTTGAATG AGGAAAATGA TGCAGGGTCT 
CAGCCGTGCG TGCCGAGAAA GAGGAGGGCT CAGAGGCTCG AGTCCTCAGA GTCTGAGCTC 
AGTGTTGAAG ATGATTCTCT TCCAACACTG GCTCATATAT TCAAATCTCA CCGTTTACCC 
TCACACTCTG GGCAGGAACC AAATCAGACC TCTGAGATTT CTGAGTTAGA TCACCAAGAA 
CCAGATCCAG GCTTTCAATC TCCAGCTGAC CAGGCTAACA AATCAAATGA AGCAACACCA 
GAGGCACAGT CTCAGAATCA GGAATGTCCT GAAAATGCAA ATGCTACAGC AGACGATGAC 
CATCTACCTC AACCTTCCTG CAGAGAGGAA TGGGTTACAT CGAGCCAAGG ATCTGTGGAT 
CTCTTCGGGA CGCCAGAAGA AAGTGAAGCC GTTGGTATCT GTGGAAATGT TGCAGTTTCC 
AGGGATTCCT CTCAGTACTC GAGTGAGATT ATCACCACAC AGCAGAAAGA GGAGATGCAG 
CAGGAGCTGT GTAGGCTGGA GAGGATGATG GCTCTGGTGT CTGAGGCTCT CCAGAAGAAG 
GAGAAGGACA CAAGCGCCAA AACCCAGAAC AAGGCTCTCA ACCCTACTCA CTCTGAACTA 
CAGTCAGCTG CTGACCCTGA CCAGGCTGAG CCGTGCAGCC CTGCACTCCC TGCAGACAGG 
CAGAGCGGCC TTGATTCTGA CGGAGATCAC AGTGGGGTGT CCCCTGGCCC TGCAGCTCCA 
GACGGCCTGT GTGCTCCCGA GTCCCAACCT CTGTTGATGA CAGACCAGGC AAAGACAGGG 
CGGCGGGTCA GCAGGAGCCG TCAGAAATCT CAGGGCAGGG GGAGGAAGTC TTCACAGAAG 
GCAGAAGCTG GGTTATCTCT CAGAAATCAC AACACCACCT CGGAGGGTTC GGTTACGGAG 
CTGACTAAGG GAAACGAGGC AAAAGAAGCC CAGAAACAAA CCCGTGGAGC GGACATGCGC 
AGACTGGGGT CCAGCAGCAC AGCAGGGAAG ATGGAGCTGG TGGCTTCTGG GCTCAGTGCC 
AGCGAATTGA TGTTGGTGAA GAAATTTGCC AGGAAAATGC ACGGCCATCT AAGTAAAGAG 
ATGACCCCCG GTACTACCCA TGTCATCATT AAGACAGATG AGGACCTCGT GTGTGAGCGC 
ACCTTAAAGT ACTTTCAGGG GATCGCCGGC AGGAAGTGGG TGGTGAGCTT CCTGTGGATC 
TCGGAGTGCT TTAATCAAGG CCAAGTTCTA GACGAGGCTC AGTTTGAAGT TCGTGGAGAT 
GTTGTTAATG GACACAACCA CAACGGGCCC TTAAAAAGCC GGACCACCTC TGATGGGTTG 
CTGATGAAAG GATACGAGAT CTGCTTCCAG GGATCCTTCA CAGCGATGAC TACAGATCAG 
ATGGAGGCTA TGGTGGAGAT GTGTGGCGCT ACTGTTGTAA AAGACCCGCT CATGTTCTCC 
AAACACATAG AAAGTAAGTG TCAGCTGGTG GTGGTGCAGC CTGGTTCTGA TGACTCTCAA 
ACTTACTACA GAGCTCTGCA GAAGAAAGCC ACAGTGGTGT CTCGTAGCTG GCTCTTGGAT 
ACCATATCAA CCTACAGCCT CCAGAATCCC CAGGACCATA AACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022525126.1
CDS	176..4843
Translation	MTAPKAEDIKRGIAVIWENLQCPICLDLMNAPVSTRCDHQFCKFCLMKLLERSKKKETSCPVCKMNVTKRSLKESPGFQRLVEGLQNLVQSYEFDTCTSYFTGIPQSRKETSVETESRDQQCSEENGSSGNDVTDKSASHFSSAAVKDAFAELMGLVDSCTVNSEQDCFDSGLGGPVNKADELQDVPSRIEDRQSNSPIPDSSGSDGKTDLHKRRSSRRPKRVGLEPDRIADKRQKKSVEKVAEWLLKISPSSDTQTRDRSRDPSVPADSDSEKESVCSSSPTEAYPKDGDEDDVNPPKEVRGRGLEEKVFGAVYKRERKGGKTKVNRPVSPEKETVTNPVPFNLVQEEEMMTRHGLKRKRSCRLTPADFIKKSSSDGREAEVKEPVDSVTHNAVEENKQVELNTEEQIEILGECDKIAELSADAKCDNTVKNMEESPAIEVPLKRSGRRSKSKMQDIWQNVDLDLKEKENICDNRTSRKRRSNRSSCELTKDGHLDESKNAKCTKSLTLVDAAANNVDVMEQLKNKPKIVEAEINIESYPSSADPRSPDARKTRRSLRLQAFTTEIQGTLKQRRTTKPSSGPETHPLDNPSHFNCLDQPNVDKVNANEENVDADQGIGSTQQSRSGKTDLLIMKNGCVFNSSLGTIEAVQTNEETATSPCVSEGQGPEQNSVLSIIPDTVAQDGPSLPCSSVAASSAFPLQAVTLDSSLPEKSQTSPSAGFVAPSVVQTLKDAHCERGGIEVEGETNDSELDTELLMKSFKASKRKSFHLGSPKPSPKTVQERLLTRTDEEQKLQYQIPTTCKDDELTSDLATICHNDLPPSSNASKLPLCEESSQRSQSSFGNAAVASLIQANASVATGDQKEAFLKNSGNSTCSGLSPNRVARSSQDSKLPTNSDSAASYQGFFPKVTSEESSICSSSEVQKNELQKSPKLGTCQPRIAEMSNKVEGSDVQTKHSSDSNKSPNIAAAGSPNLGAHSNQFESSITPDGLIPKGPVPYECQVTEPASNHSLNEENDAGSQPCVPRKRRAQRLESSESELSVEDDSLPTLAHIFKSHRLPSHSGQEPNQTSEISELDHQEPDPGFQSPADQANKSNEATPEAQSQNQECPENANATADDDHLPQPSCREEWVTSSQGSVDLFGTPEESEAVGICGNVAVSRDSSQYSSEIITTQQKEEMQQELCRLERMMALVSEALQKKEKDTSAKTQNKALNPTHSELQSAADPDQAEPCSPALPADRQSGLDSDGDHSGVSPGPAAPDGLCAPESQPLLMTDQAKTGRRVSRSRQKSQGRGRKSSQKAEAGLSLRNHNTTSEGSVTELTKGNEAKEAQKQTRGADMRRLGSSSTAGKMELVASGLSASELMLVKKFARKMHGHLSKEMTPGTTHVIIKTDEDLVCERTLKYFQGIAGRKWVVSFLWISECFNQGQVLDEAQFEVRGDVVNGHNHNGPLKSRTTSDGLLMKGYEICFQGSFTAMTTDQMEAMVEMCGATVVKDPLMFSKHIESKCQLVVVQPGSDDSQTYYRALQKKATVVSRSWLLDTISTYSLQNPQDHKP

Target ORF information:

RefSeq Version	XM_022669405.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus BRCA1, DNA repair associated (brca1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022669405.1

ORF Insert Sequence:

ATGACCGCTC CCAAAGCCGA GGACATCAAG CGGGGAATTG CTGTGATATG GGAGAATTTG 
CAGTGTCCAA TATGTTTGGA CTTGATGAAT GCACCAGTAT CGACTAGGTG TGATCACCAG 
TTCTGCAAGT TCTGCCTGAT GAAACTGCTG GAAAGGAGTA AAAAGAAAGA GACGAGCTGC 
CCTGTGTGCA AGATGAATGT CACTAAAAGG AGTCTGAAAG AAAGTCCAGG GTTCCAGAGG 
CTGGTGGAAG GACTGCAGAA TTTAGTCCAG TCTTATGAAT TTGACACCTG TACAAGCTAT 
TTTACAGGAA TACCACAGAG CAGGAAAGAA ACCAGTGTGG AAACAGAGTC CAGAGATCAG 
CAGTGCAGTG AGGAAAATGG CTCCTCAGGG AACGATGTGA CAGATAAGTC AGCATCTCAT 
TTCTCCTCGG CTGCAGTGAA GGATGCATTT GCAGAACTCA TGGGTCTTGT GGATTCCTGT 
ACAGTTAATT CAGAGCAGGA CTGTTTTGAC AGTGGTCTTG GCGGTCCTGT TAATAAAGCA 
GATGAGCTTC AAGATGTTCC TTCAAGAATA GAAGACCGAC AGAGTAATTC ACCCATACCT 
GATAGCAGCG GTTCTGATGG TAAGACTGAC CTACACAAGA GACGGTCAAG CAGAAGACCA 
AAAAGAGTAG GCTTGGAGCC AGACAGAATT GCAGACAAAA GACAAAAGAA AAGTGTGGAA 
AAAGTAGCAG AGTGGTTGCT AAAGATTTCA CCTTCATCAG ACACACAAAC CAGAGACAGG 
AGTAGAGATC CCTCAGTTCC CGCTGATAGT GATTCTGAAA AGGAGAGTGT GTGTTCATCC 
TCCCCTACAG AGGCTTATCC GAAAGATGGC GATGAGGATG ACGTCAATCC ACCAAAGGAG 
GTGCGTGGAC GTGGTCTAGA GGAAAAGGTT TTTGGGGCTG TCTATAAACG TGAACGTAAA 
GGTGGTAAAA CCAAAGTAAA CAGACCTGTC TCTCCTGAAA AAGAGACCGT GACCAACCCT 
GTTCCCTTTA ATTTGGTGCA AGAGGAAGAA ATGATGACAC GACATGGCCT TAAGAGAAAG 
AGGAGCTGTA GGCTGACTCC GGCAGATTTC ATTAAGAAAT CATCTTCTGA CGGTAGAGAA 
GCAGAAGTTA AAGAGCCCGT GGACTCTGTT ACTCATAATG CTGTGGAAGA AAACAAGCAA 
GTTGAATTAA ATACTGAGGA ACAAATAGAA ATTTTGGGAG AGTGTGACAA GATAGCAGAG 
CTCTCTGCAG ATGCAAAATG TGACAATACA GTTAAAAACA TGGAAGAAAG TCCTGCGATT 
GAAGTACCTT TGAAGAGATC AGGGAGAAGA TCAAAGTCAA AAATGCAGGA TATATGGCAA 
AATGTGGATC TTGACTTAAA AGAAAAAGAA AACATTTGTG ACAATAGAAC AAGTCGCAAG 
AGGCGGAGCA ATAGGAGCAG CTGTGAGCTG ACAAAAGATG GCCACTTGGA TGAAAGCAAG 
AATGCAAAGT GTACAAAATC CCTTACCCTT GTGGATGCGG CTGCTAATAA TGTTGATGTA 
ATGGAGCAGT TGAAAAATAA ACCAAAAATA GTGGAAGCAG AAATTAATAT TGAGAGTTAC 
CCAAGCAGTG CAGATCCACG ATCTCCTGAT GCTAGAAAAA CAAGAAGAAG TTTAAGGCTT 
CAGGCCTTCA CTACAGAAAT TCAGGGAACT CTGAAACAGA GACGAACTAC AAAACCTTCC 
AGTGGACCTG AAACTCATCC GTTAGATAAT CCATCACACT TTAATTGTTT GGACCAACCT 
AATGTGGATA AAGTGAATGC AAATGAAGAA AATGTTGATG CTGATCAGGG TATTGGAAGT 
ACTCAACAGT CTCGCAGTGG AAAAACTGAC CTGCTGATCA TGAAAAATGG ATGTGTATTC 
AACAGCAGCT TAGGAACAAT TGAGGCAGTG CAGACTAATG AGGAAACAGC TACTTCTCCA 
TGTGTTTCAG AGGGACAAGG CCCTGAACAA AATTCTGTCC TGTCCATTAT TCCAGACACA 
GTGGCTCAAG ATGGACCAAG CCTTCCTTGT TCTTCTGTGG CTGCCAGCTC TGCATTTCCA 
TTACAGGCTG TCACACTAGA CTCATCCTTA CCAGAGAAAA GCCAGACGTC TCCATCCGCA 
GGGTTCGTCG CCCCGTCTGT TGTACAGACA TTGAAAGATG CTCATTGTGA GCGTGGAGGC 
ATAGAAGTGG AAGGTGAGAC CAATGATAGT GAACTTGATA CAGAGCTTCT AATGAAGTCC 
TTCAAAGCTT CCAAAAGAAA ATCTTTCCAC CTGGGCAGCC CCAAGCCTTC ACCTAAAACA 
GTCCAGGAGA GGCTTCTAAC CAGGACTGAC GAGGAACAGA AGCTGCAATA TCAGATACCA 
ACCACTTGTA AAGACGATGA ACTAACATCA GATCTTGCAA CCATTTGCCA TAATGACTTG 
CCTCCATCTT CAAATGCTTC TAAGCTTCCG TTATGTGAGG AATCGTCTCA AAGGTCTCAG 
AGCAGTTTTG GTAATGCAGC TGTTGCAAGT CTTATCCAAG CAAATGCATC TGTTGCCACA 
GGAGATCAAA AGGAAGCTTT TCTGAAGAAC TCCGGCAACA GCACATGTTC AGGCCTAAGT 
CCCAACAGAG TTGCAAGATC TTCTCAAGAT TCCAAGCTTC CTACAAATTC TGACTCTGCA 
GCATCCTACC AGGGGTTCTT TCCTAAAGTT ACTTCAGAGG AGTCATCAAT CTGCAGCAGC 
AGCGAAGTCC AAAAGAATGA GCTCCAGAAA TCTCCAAAAT TAGGTACTTG TCAACCAAGG 
ATTGCCGAGA TGTCAAACAA AGTCGAGGGC AGCGATGTGC AGACAAAACA TTCTTCAGAC 
TCAAACAAAA GCCCAAACAT CGCAGCAGCA GGTTCACCAA ATCTTGGAGC TCACAGTAAT 
CAATTTGAAT CCTCAATCAC CCCAGATGGA CTCATACCAA AAGGTCCTGT TCCTTATGAA 
TGTCAAGTCA CAGAACCTGC ATCTAATCAC AGTTTGAATG AGGAAAATGA TGCAGGGTCT 
CAGCCGTGCG TGCCGAGAAA GAGGAGGGCT CAGAGGCTCG AGTCCTCAGA GTCTGAGCTC 
AGTGTTGAAG ATGATTCTCT TCCAACACTG GCTCATATAT TCAAATCTCA CCGTTTACCC 
TCACACTCTG GGCAGGAACC AAATCAGACC TCTGAGATTT CTGAGTTAGA TCACCAAGAA 
CCAGATCCAG GCTTTCAATC TCCAGCTGAC CAGGCTAACA AATCAAATGA AGCAACACCA 
GAGGCACAGT CTCAGAATCA GGAATGTCCT GAAAATGCAA ATGCTACAGC AGACGATGAC 
CATCTACCTC AACCTTCCTG CAGAGAGGAA TGGGTTACAT CGAGCCAAGG ATCTGTGGAT 
CTCTTCGGGA CGCCAGAAGA AAGTGAAGCC GTTGGTATCT GTGGAAATGT TGCAGTTTCC 
AGGGATTCCT CTCAGTACTC GAGTGAGATT ATCACCACAC AGCAGAAAGA GGAGATGCAG 
CAGGAGCTGT GTAGGCTGGA GAGGATGATG GCTCTGGTGT CTGAGGCTCT CCAGAAGAAG 
GAGAAGGACA CAAGCGCCAA AACCCAGAAC AAGGCTCTCA ACCCTACTCA CTCTGAACTA 
CAGTCAGCTG CTGACCCTGA CCAGGCTGAG CCGTGCAGCC CTGCACTCCC TGCAGACAGG 
CAGAGCGGCC TTGATTCTGA CGGAGATCAC AGTGGGGTGT CCCCTGGCCC TGCAGCTCCA 
GACGGCCTGT GTGCTCCCGA GTCCCAACCT CTGTTGATGA CAGACCAGGC AAAGACAGGG 
CGGCGGGTCA GCAGGAGCCG TCAGAAATCT CAGGGCAGGG GGAGGAAGTC TTCACAGAAG 
GCAGAAGCTG GGTTATCTCT CAGAAATCAC AACACCACCT CGGAGGGTTC GGTTACGGAG 
CTGACTAAGG GAAACGAGGC AAAAGAAGCC CAGAAACAAA CCCGTGGAGC GGACATGCGC 
AGACTGGGGT CCAGCAGCAC AGCAGGGAAG ATGGAGCTGG TGGCTTCTGG GCTCAGTGCC 
AGCGAATTGA TGTTGGTGAA GAAATTTGCC AGGAAAATGC ACGGCCATCT AAGTAAAGAG 
ATGACCCCCG GTACTACCCA TGTCATCATT AAGACAGATG AGGACCTCGT GTGTGAGCGC 
ACCTTAAAGT ACTTTCAGGG GATCGCCGGC AGGAAGTGGG TGGTGAGCTT CCTGTGGATC 
TCGGAGTGCT TTAATCAAGG CCAAGTTCTA GACGAGGCTC AGTTTGAAGT TCGTGGAGAT 
GTTGTTAATG GACACAACCA CAACGGGCCC TTAAAAAGCC GGACCACCTC TGATGGGTTG 
CTGATGAAAG GATACGAGAT CTGCTTCCAG GGATCCTTCA CAGCGATGAC TACAGATCAG 
ATGGAGGCTA TGGTGGAGAT GTGTGGCGCT ACTGTTGTAA AAGACCCGCT CATGTTCTCC 
AAACACATAG AAAGTAAGTG TCAGCTGGTG GTGGTGCAGC CTGGTTCTGA TGACTCTCAA 
ACTTACTACA GAGCTCTGCA GAAGAAAGCC ACAGTGGTGT CTCGTAGCTG GCTCTTGGAT 
ACCATATCAA CCTACAGCCT CCAGAATCCC CAGGACCATA AACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brca1 ( XM_015602449.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_015457935.1 Astyanax mexicanus breast cancer 1 (brca1), mRNA.

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CloneID	OAs21638
Clone ID Related Accession (Same CDS sequence)	XM_015602449.1
Accession Version	XM_015602449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	breast cancer type 1 susceptibility protein
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006739152.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGACCGCTC CAAAAGCCGA GGACATCAAG CGGGGAATTG CTGTGATATG GGAGAATTTG 
CAGTGTCCAA TATGTTTGGA CTTGATGAAT GCACCAGTAT CGACTAGGTG TGATCACCAG 
TTCTGCAAGT TCTGCCTGAT GAAACTGCTG GAAAGGAGTA AAAAGAAAGA GACGAGCTGC 
CCTGTGTGCA AGATGAATGT CACTAAAAGG AGTCTGAAAG AAAGTCCAGG GTTCCAGAGG 
CTGGTGGAAG GACTGCAGAA TTTAGTCCAG TCTTATGAAT TTGACACCTG TACAAGCTAT 
TTTACAGGAA TACCACAGAG CAGGAGAGAA ACCAGTGTGG AAACAGAGTC CAGAGATCAG 
CAGTGCAGTG AGGAAAATGG CTCCTCAGGG AACGATGTGA CAGATAAGTC AGCATCTCAT 
TTCTCCTCGG CTGCAGTGAA GGATGCATTT GCAGAACTCA TGGGTCTTGT GGATTCCTGT 
ACAGTTAATT CAGAGCAGGA CTGTTTTGAC AGTGGTCTTG GCGGTCCTGT TAATAAAGCA 
GATGAGCTTC AAGATGTTCC TTCAAGAATA GAAGACAGAC AGGGTAATTC ACCCATACCT 
GATAGCAGCG GTTCTGATGG TAAGACTGAC CTACACAAGA GACGGTCAAG CAGAAGACCA 
AAAAGAGTAG GCTTGGAGCC AGACAGAATT GCAGACAAAA GACAAAAGAA AAGTGTGGAA 
AAAGTAGCAG AGTGGTTGCT AAAGATTTCA CCTTCATCAG ACACACAAAC CAGAGACAGG 
AGTAGAGATC CCTCAGTTCC CGCTGATAGT GATTCTGAGA AGGAGAGTGT GTGTTCATCC 
TCCCCTACAG AGGCTTATCC GAAAGATGGC GATGAGGATG ACGTCAATCC ACCAAAGGAG 
GTGCGTGGAC GTGGTCTAGA GGAAAAGGTT TTTGGGGCTG TCTATAAACG TGAACGTAAA 
GGTGGTAAAA CCAAAGTAAA CAGACCTGTC TCTCCTGAAA AAGAGACCGT GACCAACCCT 
GTTCCCTTTA ATTTGGTGCA AGAGGAAGAA ATGATGACAC GACATGGCCT TAAGAGAAAG 
AGGAGCTGTA GGCTGACTCC GGCAGATTTC ATTAAGAAAT CATCTTCTGA CGGTAGAGAA 
GCAGAAGTTA AAGAGCCCGT GGACTCTGTT ACTCATAATG CTGTGGAAGA AAACAAGCAA 
GTTGAATTAA ATACTGAGGA ACAAATCGAA ATTTTGGGGG AGTGTGACAA GATAGCAGAG 
CTCTCTGCAG ATGCAAAATG TGACAATACA GTTAAAAACA TGGAAGAAAG TCCTGCGATT 
GAAGTACCTT TGAAGAGATC AGGGAGAAGA TCAAAGTCAA AAATGCAGGA TATATGGCAA 
AATGTGGATC TTGACTTAAA AGAAAAAGAA AACATTTGTG ACAATAGAAC AAGTCGCAAG 
AGGCGGAGCA ATAGGAGCAG CTGTGAGCTG ACAAAAGATG GCCACTTGGA TGAAAGCAAG 
AATGCAAAGT GTACAAAATC CCTTACCCTT GTGGATGCAG CTGCTAATAA TGTTGATGTA 
ATGGAGCAGT TGAAAAATAA ACCAAAAATA GTGGAAGCAG AAATTAATAT TGAGAGTTAC 
CCAAGCAGTG CAGATCCACA ATCTCCTGAT GCTAGAAAAA CAAGAAGAAG TTTGAGGCTT 
CAGGCCTTCA CTACAGAAAT TCAGGGAACT CTGAAACAGA GACGAACTAC AAAACCTTCC 
AGTGGACCTG AAACTCATCC GTTAGATAAT CCATCACACT TTAATTGTTT GGACCAACCT 
AATGTGGATA AAGTGAATGC AAATGAAGAA AATGTTGATG CTGATCAGGG TATTGGAAGT 
ACTCAACAGT CTCGCAGTGG AAAAACTGAC CTGCTGATCA TGAAAAATGG ATGTGTATTC 
AACAGCAGCT TAGGAACAAT TGAGGCAGTG CAGACTAATG AGGAAACAGC TTCTTCTCCA 
TGTGTTTCAG AGGGACAAGG CCCTGAACAA AATTCTGTCC TGTCCATTAT TCCAGACACA 
GTGGCTCAAG ATGGACCAAG CCTTCCTTGT TCTTCCGTGG CTGCCAGCTC TGCATTTCCA 
TTACAGGCTG TCACACTAGA CTCATCCTTA CCAGAGAAAA GCCAGACGTC TCCATCCGCA 
GGGTTCGTCG CCCCGTCTGT TGTACAGACA TTGAGAGATG CTCATTGTGA GCGTGGAGGC 
ATAGAAGTGG AAGGTGAGAC CAATGATAGT GAACTTGATA CAGAGCTTCT AATGAAGTCC 
TTCAAAGCTT CCAAAAGAAA ATCTTTCCAC CTGGGCAGCC CCAAGCCTTC ACCTAAAACA 
GTCCAGGAGA GGCTTCTAAA CAGGACTGAC GAGGAACAGA AGCTGCAATA TCAGATACCA 
ACCACTTGTA AAGACGATGA ACTAACATCA GATCTTGCAA CCATTTGCCA TAATGACTTG 
CCTCCATCTT CAAATGCTTC TAAGCTTCCG TTATGTGAGG AATCGTCTCA AAGGTCTCAG 
AGCAGTTTTG GTAATGCAGC TGTTGCAAGT CTTATCCAAG CAAATGCATC TGTTGCCACA 
GGAGATCAAA AGGAAGCTTT TCTGAAGAAC TCCGGCAACA GCACATGTTC AGGCCTAAGT 
CCCAACAGAG TTGGAAGATC TTCTCAAGAT TCCAAGCTTC CTACAAATTC TGACTCTGCA 
GCATCCTACC AGGGGTTCTT TCCTAAAGTT ACTTCAGAGG AGTCATCAAT CTGCAGCAGC 
AGCGAAGTCC AAAAGAATGA GCTCCAGAAA TCTCCAAAAT TAGGTACTTG TCAACCAAGG 
ATTGCCGAGA TGTCAAACAA AGTCGAAGGC AGCGATGTGC AGACAAAACA TTCTTCAGAC 
TCAAACAAAA GCCCAAACAT CGCAGCAGCA GGTTCACCAA ATCTTGGAGC TCACAGTAAT 
CAATTTGAAT CCTCAATCAC CCCAGATGGA CTCATACCAA AAGGTCCTGT TCCTTATGAA 
TGTCAAGTCA CAGAACCTGC ATCTTATCAC AGTTTGAATG AGGAAAAGGA TGCAGGGTCT 
CAGCCGTGCG TGCCGAGAAA GAGGAGGGCT CAGAGGCTCG AGTCCTCAGA GTCTGAGCTC 
AGTGTTGAAG ATGATTCTCT TCCCACACTG GCTCATATAT TCAAACCTCA CCGTTTACCC 
TCACACTCTG GGCAGGAACC AAATCAGACC TCTGAGATTT CTGAGTTAGA TCACCAAGAA 
CCAGATCCAG GCTATCAATC TCCAGCTGAC CAAGAACCAG ATCCAGGCTA TCAATCTCCG 
GCTGACCAAG AACCAGATCC AGGCTATCAA TCTCCAGCTG ACCAAGAACC AGATCCAGGC 
TATCAATCTC CAGCTGACCA GGCTAACAAA TCACTCTCTC TGCAGCAGAA AGAGGAGATG 
CAGCAGGAGC TGTGTAGGCT GGAGAGGATG ATGGCTCTGG TGTCTGAGGC TCTCCAGAAG 
AAGGAGAAGG ACACAAGCGC CAAAACCCAG AACAAGGCTC TCAACCCTAC TCACTCTGAA 
CTACAGTCAG CTGCTGACCC TGACCAGGCT GAGCCGTGCA GCCCTGCACT CCCTGCAGAC 
AGGCAGAGCG GCCTTGATTC TGACGGAGAT CACAGTGGGG TGTCCCCTGG CCCTGCAGCT 
CCAGACGGCC TGTGTGCTCC CGAGTCCCAA CCTCTGTTGA CGACAGACCA GGCAAAGACA 
GGGCGGCGGG TCAGCAGGAG CCGTCAGAAA TCTCAGGGCA GGGGGAGGAA GTCTTCACAG 
AAGGCAGAAG CTGGGTTATC TCTCAGAAAT CACAACACCA CCTCGGAGGG TTCGGTTACG 
GAGCTGACTA AGGGAAACGA GGCAAAAGAA GCCCAGAAAC AAACCCGTGG AGCGGACATG 
CGCAGACTGG GGTCCAGCAG CACAGCAGGG AAGATGGAGC TGGTGGCTTC TGGGCTCAGT 
GCCAGCGAAT TGATGTTGGT GAAGAAATTT GCCAGGAAAA TGCACGGCCA TCTAAGTAAA 
GAGATGACCC CCGGTACTAC CCATGTCATC ATTAAGACAG ATGAGGACCT CGTGTGTGAG 
CGCACCTTAA AGTACTTTCA GGGGATCGCC GGCAGGAAGT GGGTGGTGAG CTTCCTGTGG 
ATCTCGGAGT GCTTTAATCA AGGCCAAGTT CTAGACGAGG CTCAGTTTGA AGTTCGTGGA 
GATGTTGTTA ATGGACACAA CCACAACGGG CCCTTAAAAA GCCGGACCAC CTCTGATGGG 
TTGCTGATGA AAGGATACGA GATCTGCTTC CAGGGATCCT TCACAGCGAT GACTACAGAT 
CAGATGGAGG CTATGGTGGA GATGTGTGGC GCTACTGTTG TAAAAGACCC GCTCATGTTC 
TCCAAACACA TAGAAAGTAA GTGTCAGCTG GTGGTGGTGC AGCCTGGTTC TGATGACTCT 
CAAACTTACT ACAGAGCTCT GCAGAAGAAA GCCACAGTGG TGTCTCGTAG CTGGCTCTTG 
GATACCATAT CAACCTACAG CCTCCAGAAT CCCCAGGACC ATAAACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015457935.1
CDS	173..4723
Translation	MTAPKAEDIKRGIAVIWENLQCPICLDLMNAPVSTRCDHQFCKFCLMKLLERSKKKETSCPVCKMNVTKRSLKESPGFQRLVEGLQNLVQSYEFDTCTSYFTGIPQSRRETSVETESRDQQCSEENGSSGNDVTDKSASHFSSAAVKDAFAELMGLVDSCTVNSEQDCFDSGLGGPVNKADELQDVPSRIEDRQGNSPIPDSSGSDGKTDLHKRRSSRRPKRVGLEPDRIADKRQKKSVEKVAEWLLKISPSSDTQTRDRSRDPSVPADSDSEKESVCSSSPTEAYPKDGDEDDVNPPKEVRGRGLEEKVFGAVYKRERKGGKTKVNRPVSPEKETVTNPVPFNLVQEEEMMTRHGLKRKRSCRLTPADFIKKSSSDGREAEVKEPVDSVTHNAVEENKQVELNTEEQIEILGECDKIAELSADAKCDNTVKNMEESPAIEVPLKRSGRRSKSKMQDIWQNVDLDLKEKENICDNRTSRKRRSNRSSCELTKDGHLDESKNAKCTKSLTLVDAAANNVDVMEQLKNKPKIVEAEINIESYPSSADPQSPDARKTRRSLRLQAFTTEIQGTLKQRRTTKPSSGPETHPLDNPSHFNCLDQPNVDKVNANEENVDADQGIGSTQQSRSGKTDLLIMKNGCVFNSSLGTIEAVQTNEETASSPCVSEGQGPEQNSVLSIIPDTVAQDGPSLPCSSVAASSAFPLQAVTLDSSLPEKSQTSPSAGFVAPSVVQTLRDAHCERGGIEVEGETNDSELDTELLMKSFKASKRKSFHLGSPKPSPKTVQERLLNRTDEEQKLQYQIPTTCKDDELTSDLATICHNDLPPSSNASKLPLCEESSQRSQSSFGNAAVASLIQANASVATGDQKEAFLKNSGNSTCSGLSPNRVGRSSQDSKLPTNSDSAASYQGFFPKVTSEESSICSSSEVQKNELQKSPKLGTCQPRIAEMSNKVEGSDVQTKHSSDSNKSPNIAAAGSPNLGAHSNQFESSITPDGLIPKGPVPYECQVTEPASYHSLNEEKDAGSQPCVPRKRRAQRLESSESELSVEDDSLPTLAHIFKPHRLPSHSGQEPNQTSEISELDHQEPDPGYQSPADQEPDPGYQSPADQEPDPGYQSPADQEPDPGYQSPADQANKSLSLQQKEEMQQELCRLERMMALVSEALQKKEKDTSAKTQNKALNPTHSELQSAADPDQAEPCSPALPADRQSGLDSDGDHSGVSPGPAAPDGLCAPESQPLLTTDQAKTGRRVSRSRQKSQGRGRKSSQKAEAGLSLRNHNTTSEGSVTELTKGNEAKEAQKQTRGADMRRLGSSSTAGKMELVASGLSASELMLVKKFARKMHGHLSKEMTPGTTHVIIKTDEDLVCERTLKYFQGIAGRKWVVSFLWISECFNQGQVLDEAQFEVRGDVVNGHNHNGPLKSRTTSDGLLMKGYEICFQGSFTAMTTDQMEAMVEMCGATVVKDPLMFSKHIESKCQLVVVQPGSDDSQTYYRALQKKATVVSRSWLLDTISTYSLQNPQDHKP

Target ORF information:

RefSeq Version	XM_015602449.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus breast cancer 1 (brca1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015602449.1

ORF Insert Sequence:

ATGACCGCTC CAAAAGCCGA GGACATCAAG CGGGGAATTG CTGTGATATG GGAGAATTTG 
CAGTGTCCAA TATGTTTGGA CTTGATGAAT GCACCAGTAT CGACTAGGTG TGATCACCAG 
TTCTGCAAGT TCTGCCTGAT GAAACTGCTG GAAAGGAGTA AAAAGAAAGA GACGAGCTGC 
CCTGTGTGCA AGATGAATGT CACTAAAAGG AGTCTGAAAG AAAGTCCAGG GTTCCAGAGG 
CTGGTGGAAG GACTGCAGAA TTTAGTCCAG TCTTATGAAT TTGACACCTG TACAAGCTAT 
TTTACAGGAA TACCACAGAG CAGGAGAGAA ACCAGTGTGG AAACAGAGTC CAGAGATCAG 
CAGTGCAGTG AGGAAAATGG CTCCTCAGGG AACGATGTGA CAGATAAGTC AGCATCTCAT 
TTCTCCTCGG CTGCAGTGAA GGATGCATTT GCAGAACTCA TGGGTCTTGT GGATTCCTGT 
ACAGTTAATT CAGAGCAGGA CTGTTTTGAC AGTGGTCTTG GCGGTCCTGT TAATAAAGCA 
GATGAGCTTC AAGATGTTCC TTCAAGAATA GAAGACAGAC AGGGTAATTC ACCCATACCT 
GATAGCAGCG GTTCTGATGG TAAGACTGAC CTACACAAGA GACGGTCAAG CAGAAGACCA 
AAAAGAGTAG GCTTGGAGCC AGACAGAATT GCAGACAAAA GACAAAAGAA AAGTGTGGAA 
AAAGTAGCAG AGTGGTTGCT AAAGATTTCA CCTTCATCAG ACACACAAAC CAGAGACAGG 
AGTAGAGATC CCTCAGTTCC CGCTGATAGT GATTCTGAGA AGGAGAGTGT GTGTTCATCC 
TCCCCTACAG AGGCTTATCC GAAAGATGGC GATGAGGATG ACGTCAATCC ACCAAAGGAG 
GTGCGTGGAC GTGGTCTAGA GGAAAAGGTT TTTGGGGCTG TCTATAAACG TGAACGTAAA 
GGTGGTAAAA CCAAAGTAAA CAGACCTGTC TCTCCTGAAA AAGAGACCGT GACCAACCCT 
GTTCCCTTTA ATTTGGTGCA AGAGGAAGAA ATGATGACAC GACATGGCCT TAAGAGAAAG 
AGGAGCTGTA GGCTGACTCC GGCAGATTTC ATTAAGAAAT CATCTTCTGA CGGTAGAGAA 
GCAGAAGTTA AAGAGCCCGT GGACTCTGTT ACTCATAATG CTGTGGAAGA AAACAAGCAA 
GTTGAATTAA ATACTGAGGA ACAAATCGAA ATTTTGGGGG AGTGTGACAA GATAGCAGAG 
CTCTCTGCAG ATGCAAAATG TGACAATACA GTTAAAAACA TGGAAGAAAG TCCTGCGATT 
GAAGTACCTT TGAAGAGATC AGGGAGAAGA TCAAAGTCAA AAATGCAGGA TATATGGCAA 
AATGTGGATC TTGACTTAAA AGAAAAAGAA AACATTTGTG ACAATAGAAC AAGTCGCAAG 
AGGCGGAGCA ATAGGAGCAG CTGTGAGCTG ACAAAAGATG GCCACTTGGA TGAAAGCAAG 
AATGCAAAGT GTACAAAATC CCTTACCCTT GTGGATGCAG CTGCTAATAA TGTTGATGTA 
ATGGAGCAGT TGAAAAATAA ACCAAAAATA GTGGAAGCAG AAATTAATAT TGAGAGTTAC 
CCAAGCAGTG CAGATCCACA ATCTCCTGAT GCTAGAAAAA CAAGAAGAAG TTTGAGGCTT 
CAGGCCTTCA CTACAGAAAT TCAGGGAACT CTGAAACAGA GACGAACTAC AAAACCTTCC 
AGTGGACCTG AAACTCATCC GTTAGATAAT CCATCACACT TTAATTGTTT GGACCAACCT 
AATGTGGATA AAGTGAATGC AAATGAAGAA AATGTTGATG CTGATCAGGG TATTGGAAGT 
ACTCAACAGT CTCGCAGTGG AAAAACTGAC CTGCTGATCA TGAAAAATGG ATGTGTATTC 
AACAGCAGCT TAGGAACAAT TGAGGCAGTG CAGACTAATG AGGAAACAGC TTCTTCTCCA 
TGTGTTTCAG AGGGACAAGG CCCTGAACAA AATTCTGTCC TGTCCATTAT TCCAGACACA 
GTGGCTCAAG ATGGACCAAG CCTTCCTTGT TCTTCCGTGG CTGCCAGCTC TGCATTTCCA 
TTACAGGCTG TCACACTAGA CTCATCCTTA CCAGAGAAAA GCCAGACGTC TCCATCCGCA 
GGGTTCGTCG CCCCGTCTGT TGTACAGACA TTGAGAGATG CTCATTGTGA GCGTGGAGGC 
ATAGAAGTGG AAGGTGAGAC CAATGATAGT GAACTTGATA CAGAGCTTCT AATGAAGTCC 
TTCAAAGCTT CCAAAAGAAA ATCTTTCCAC CTGGGCAGCC CCAAGCCTTC ACCTAAAACA 
GTCCAGGAGA GGCTTCTAAA CAGGACTGAC GAGGAACAGA AGCTGCAATA TCAGATACCA 
ACCACTTGTA AAGACGATGA ACTAACATCA GATCTTGCAA CCATTTGCCA TAATGACTTG 
CCTCCATCTT CAAATGCTTC TAAGCTTCCG TTATGTGAGG AATCGTCTCA AAGGTCTCAG 
AGCAGTTTTG GTAATGCAGC TGTTGCAAGT CTTATCCAAG CAAATGCATC TGTTGCCACA 
GGAGATCAAA AGGAAGCTTT TCTGAAGAAC TCCGGCAACA GCACATGTTC AGGCCTAAGT 
CCCAACAGAG TTGGAAGATC TTCTCAAGAT TCCAAGCTTC CTACAAATTC TGACTCTGCA 
GCATCCTACC AGGGGTTCTT TCCTAAAGTT ACTTCAGAGG AGTCATCAAT CTGCAGCAGC 
AGCGAAGTCC AAAAGAATGA GCTCCAGAAA TCTCCAAAAT TAGGTACTTG TCAACCAAGG 
ATTGCCGAGA TGTCAAACAA AGTCGAAGGC AGCGATGTGC AGACAAAACA TTCTTCAGAC 
TCAAACAAAA GCCCAAACAT CGCAGCAGCA GGTTCACCAA ATCTTGGAGC TCACAGTAAT 
CAATTTGAAT CCTCAATCAC CCCAGATGGA CTCATACCAA AAGGTCCTGT TCCTTATGAA 
TGTCAAGTCA CAGAACCTGC ATCTTATCAC AGTTTGAATG AGGAAAAGGA TGCAGGGTCT 
CAGCCGTGCG TGCCGAGAAA GAGGAGGGCT CAGAGGCTCG AGTCCTCAGA GTCTGAGCTC 
AGTGTTGAAG ATGATTCTCT TCCCACACTG GCTCATATAT TCAAACCTCA CCGTTTACCC 
TCACACTCTG GGCAGGAACC AAATCAGACC TCTGAGATTT CTGAGTTAGA TCACCAAGAA 
CCAGATCCAG GCTATCAATC TCCAGCTGAC CAAGAACCAG ATCCAGGCTA TCAATCTCCG 
GCTGACCAAG AACCAGATCC AGGCTATCAA TCTCCAGCTG ACCAAGAACC AGATCCAGGC 
TATCAATCTC CAGCTGACCA GGCTAACAAA TCACTCTCTC TGCAGCAGAA AGAGGAGATG 
CAGCAGGAGC TGTGTAGGCT GGAGAGGATG ATGGCTCTGG TGTCTGAGGC TCTCCAGAAG 
AAGGAGAAGG ACACAAGCGC CAAAACCCAG AACAAGGCTC TCAACCCTAC TCACTCTGAA 
CTACAGTCAG CTGCTGACCC TGACCAGGCT GAGCCGTGCA GCCCTGCACT CCCTGCAGAC 
AGGCAGAGCG GCCTTGATTC TGACGGAGAT CACAGTGGGG TGTCCCCTGG CCCTGCAGCT 
CCAGACGGCC TGTGTGCTCC CGAGTCCCAA CCTCTGTTGA CGACAGACCA GGCAAAGACA 
GGGCGGCGGG TCAGCAGGAG CCGTCAGAAA TCTCAGGGCA GGGGGAGGAA GTCTTCACAG 
AAGGCAGAAG CTGGGTTATC TCTCAGAAAT CACAACACCA CCTCGGAGGG TTCGGTTACG 
GAGCTGACTA AGGGAAACGA GGCAAAAGAA GCCCAGAAAC AAACCCGTGG AGCGGACATG 
CGCAGACTGG GGTCCAGCAG CACAGCAGGG AAGATGGAGC TGGTGGCTTC TGGGCTCAGT 
GCCAGCGAAT TGATGTTGGT GAAGAAATTT GCCAGGAAAA TGCACGGCCA TCTAAGTAAA 
GAGATGACCC CCGGTACTAC CCATGTCATC ATTAAGACAG ATGAGGACCT CGTGTGTGAG 
CGCACCTTAA AGTACTTTCA GGGGATCGCC GGCAGGAAGT GGGTGGTGAG CTTCCTGTGG 
ATCTCGGAGT GCTTTAATCA AGGCCAAGTT CTAGACGAGG CTCAGTTTGA AGTTCGTGGA 
GATGTTGTTA ATGGACACAA CCACAACGGG CCCTTAAAAA GCCGGACCAC CTCTGATGGG 
TTGCTGATGA AAGGATACGA GATCTGCTTC CAGGGATCCT TCACAGCGAT GACTACAGAT 
CAGATGGAGG CTATGGTGGA GATGTGTGGC GCTACTGTTG TAAAAGACCC GCTCATGTTC 
TCCAAACACA TAGAAAGTAA GTGTCAGCTG GTGGTGGTGC AGCCTGGTTC TGATGACTCT 
CAAACTTACT ACAGAGCTCT GCAGAAGAAA GCCACAGTGG TGTCTCGTAG CTGGCTCTTG 
GATACCATAT CAACCTACAG CCTCCAGAAT CCCCAGGACC ATAAACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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brca1 cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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