Def2 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	Def2
Entrez Gene ID	413397
Full Name	defensin 2
Synonyms	Defensin-2,GB10036
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001011638.1	NP_001011638.1	defensin-2 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Identification and characterization of the parasitic wasp Nasonia defensins: positive selection targeting the functional region?
Gao B, Zhu S
Developmental and comparative immunology34(6)659-68(2010 Jun)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

Two structurally different defensin genes, one of them encoding a novel defensin isoform, are expressed in honeybee Apis mellifera.
Klaudiny J, Albert S, Bachanová K, Kopernický J, Simúth J
Insect biochemistry and molecular biology35(1)11-22(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Def2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Def2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd00235	NM_001011638.1 Latest version!	Apis mellifera defensin 2 (Def2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Def2 ( NM_001011638.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001011638.1 Apis mellifera defensin 2 (Def2), mRNA.

Price & Availability↑

CloneID	OHd00235
Clone ID Related Accession (Same CDS sequence)	NM_001011638.1
Accession Version	NM_001011638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Apis mellifera(honey bee)
Product	defensin-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY588474.1. On Feb 5, 2005 this sequence version replaced XM_396841.1. ##Evidence-Data-START## Transcript exon combination :: AY588474.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00996631, SAMN01985568 [ECO:0000348] ##Evidence-Data-END##

ATGAAGTTCT TTGTACTTTT TGCGATTCTC ATTGCTATCG TTCACGCATC TTGCGCAAGT 
GTTCCTAAAG TTGTTTACGA TGGACCGATT TACGAGTTGA GGCAAATTGA GGAGGAAAAT 
ATAGAACCAG ATACAGAATT GATGGATTCC AACGAACCGC TGCTACCACT ACGACATCGA 
AGGGTAACGT GCGACGTTTT ATCATGGCAA TCAAAATGGC TGAGCATTAA TCATTCAGCT 
TGCGCTATCA GATGTTTAGC TCAACGACGT AAAGGCGGTA GTTGCAGAAA TGGCGTGTGT 
ATCTGTCGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011638.1
CDS	17..331
Translation	MKFFVLFAILIAIVHASCASVPKVVYDGPIYELRQIEEENIEPDTELMDSNEPLLPLRHRRVTCDVLSWQSKWLSINHSACAIRCLAQRRKGGSCRNGVCICRK

Target ORF information:

RefSeq Version	NM_001011638.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera defensin 2 (Def2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011638.1

ORF Insert Sequence:

ATGAAGTTCT TTGTACTTTT TGCGATTCTC ATTGCTATCG TTCACGCATC TTGCGCAAGT 
GTTCCTAAAG TTGTTTACGA TGGACCGATT TACGAGTTGA GGCAAATTGA GGAGGAAAAT 
ATAGAACCAG ATACAGAATT GATGGATTCC AACGAACCGC TGCTACCACT ACGACATCGA 
AGGGTAACGT GCGACGTTTT ATCATGGCAA TCAAAATGGC TGAGCATTAA TCATTCAGCT 
TGCGCTATCA GATGTTTAGC TCAACGACGT AAAGGCGGTA GTTGCAGAAA TGGCGTGTGT 
ATCTGTCGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification and characterization of the parasitic wasp Nasonia defensins: positive selection targeting the functional region?
Developmental and comparative immunology34(6)659-68(2010 Jun)
Gao B,Zhu S

Insights into social insects from the genome of the honeybee Apis mellifera.
Nature443(7114)931-49(2006 Oct)

Two structurally different defensin genes, one of them encoding a novel defensin isoform, are expressed in honeybee Apis mellifera.
Insect biochemistry and molecular biology35(1)11-22(2005 Jan)
Klaudiny J,Albert S,Bachanová K,Kopernický J,Simúth J