KIT cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene
Clones
gRNAs

Summary

Gene Symbol	KIT
Entrez Gene ID	100327263
Full Name	KIT proto-oncogene receptor tyrosine kinase
Synonyms	C-KIT
Gene Type	protein-coding
Organism	Oryctolagus cuniculus(rabbit)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017347545.1	XP_017203034.1	mast/stem cell growth factor receptor Kit
NM_001329070.1	NP_001315999.1	mast/stem cell growth factor receptor Kit

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The KIT gene is associated with the english spotting coat color locus and congenital megacolon in Checkered Giant rabbits (Oryctolagus cuniculus).
Fontanesi L, Vargiolu M, Scotti E, Latorre R, Faussone Pellegrini MS, Mazzoni M, Asti M, Chiocchetti R, Romeo G, Clavenzani P, De Giorgio R
PloS one9(4)e93750(2014)

KIT/KIT ligand in mammalian oogenesis and folliculogenesis: roles in rabbit and murine ovarian follicle activation and oocyte growth.
Hutt KJ, McLaughlin EA, Holland MK
Biology of reproduction75(3)421-33(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KIT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KIT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOb41866	NM_001329070.1 Latest version!	Oryctolagus cuniculus KIT proto-oncogene, receptor tyrosine kinase (KIT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OOb37161	XM_017347545.1 Latest version!	Oryctolagus cuniculus KIT proto-oncogene receptor tyrosine kinase (KIT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KIT ( NM_001329070.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> NP_001315999.1 Oryctolagus cuniculus KIT proto-oncogene, receptor tyrosine kinase (KIT), mRNA.

Price & Availability↑

CloneID	OOb41866
Clone ID Related Accession (Same CDS sequence)	NM_001329070.1
Accession Version	NM_001329070.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548259200000
Organism	Oryctolagus cuniculus(rabbit)
Product	mast/stem cell growth factor receptor Kit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KJ495954.1. ##Evidence-Data-START## Transcript exon combination :: KJ495954.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1876818, SAMEA1876819 [ECO:0000350] ##Evidence-Data-END##

ATGAGCAAGC ACTACCATGC AGGCTCTTCG CAGCCATCTG CGAGCCCAGG GCAGCCGGCT 
CCCCCCGTCA TCCAGCCAGC ACAGCCGGAG CTGACCGTGG ATGCCGGCAG CAGGTTCTCG 
CTGGAGTGCA TCGACCCGGG CTCCGTCAGG TGGACTTTCG AGAGCCTGGG TCAGCTGAGT 
GACAGCGAGC ACAGCCAATG GACGCGCGAG GAGGCGGAGG CCGCCAACAC GGGCAGGTAC 
ACCTGCACCA ACGCGGGCGG CCTGAGCAGC TCCATCTACG TGTTCGTGAG AGATCCGCGG 
CAGCTGTTCC TCGTGGGCCT GCCCCTGTAC GGCCGAGAAG ACAGTGACAC GCTGGTCCGC 
TGTCCGCTGA CCGACCCGGA CGTGACCAAC TACACCCTCA AGGAGTGCGA GGGGAAGCCT 
CTCCCCAAGG ACTTGACCTT CGTCCCGGAC CCCAAGGCCG GCATCACCAT CAAGAACGTG 
AGGCGCGCCT ACCACCCCCT GTGCCTGCCC TGCGCCGCCG ACCGCGAGGG CACAACCGTG 
CAGTCGGAAA AATTCATCCT GAAAGTGAGA GCAGCCGCAG CCATCAAGGC CGTCCCGGTG 
GTGTCCGTGT CTAGGACGAG CTGCCTGCTT AGAGAGGGGG AAGAGTTCTC AGTGACCTGC 
ACGGTCAGAG ACGTGTCCAC CGCCGTGCTG TCCGCGTGGC TGAAGGAGGA CGGCCAGCGG 
ACAAACGGCC AGGTGAGGCG CCACAGCTGG CATCAGGGCG ACTTCCACTA CGAGCGCCAG 
GAGACGCTGA CGATCGGCGC GGCGAGAGTG GCGGACTCCG GGGTGTTCAT GTGCTACGCC 
AACAACACCT TCGGGTCGGC GAATGTCACC ACGACCTTGA AAGTCGTAGA TAAAGGCTTC 
ATCCGGGTCT TCTCGGTGAC CAACACCACC ATGTTTGTCA ACGACGGAGA AAACGTGGAC 
TTGGTGGTGC AGTACGAGGC GTACCCGCGG CCCACGCAGC AGCAGTGGCT GTACATGAAC 
AGGACCTCCG CCAGCAAGTG GGAGGACCAC CCAGAGTCAG AGAATGAGAG CAACATCAGA 
TACGTGAGCA AGCTGCACCT GACCAGACTG AAGGGGAGCG AGGCCGGCAC CTACACGTTT 
CTCGTGTCCA ATGCTGATGT CAACGCTTCC GTGGCCTTTA ACGTTTACGT GAACACGAAA 
CCAAAGATCC TGACTTCCAA AAGACTGGTG AACGGCGTGC TGCAGTGTGA GGCAGCAGGA 
TTCCCGGAGC CCACCATAGA CTGGTATTTC TGTCTGGGAA CCGAGCAGAG GTGCTCGGCG 
CCGGTGTGGC CGGTGGCCGT GCAGGTCCAG AATGCGTCGG AGCAGCCCTT CGGGAAGCCG 
GTGGTCCAGA GCGCCATCGA CTCGAGCGCC TTCAAGCACA ATGGCACGGT GGAGTGCAGG 
GCGTCCAACG ACGCGGGCCA GAGCTCCGCC TACTTCAACT TTGCCCTTAA AGGTAACAGC 
AAAGGTATAC TCCTCTTTAA TCCAATTTCA GTCATATACT CACTCACGTG TGTGCATGCG 
TGTGTGTGTC TTTATGTAAC TGTATATTTA CAGCTTGACT CCATTAAGAT GGGCAGAGGA 
AAAAAGGGGA ATGCAATCAC TTTTATCTTT ACTTCCCGCC CCCCGCCCCC CACCACTGAC 
CCTGTCCCCA TCCGTCCTGT TCCTCTCGCA GGGAAGACCC TGGGCGCCGG CGCCTTCGGG 
AAGGTGGTGG AGGCCACGGC CTACGGGCTG ATCAAGTCGG ATGCGGCCAT GACCGTCGCT 
GTGAAGATGC TGAAACCGAG CGCCCACCTG ACGGAGCGGG AAGCCCTCAT GTCCGAGCTC 
AAGGTGCTGA GCTACCTGGG GAATCACATG AACATCGTGA ACCTCCTGGG AGCCTGCACC 
GTCGGAGGGC CCACCCTGGT CATTACAGAG TACTGCTGCT ACGGGGACCT CCTGAACTTC 
CTGCGGAGAA AGCGGGATTC GTTCATCTGC TCCAAGCAGG AGGACCACGG GGAAGCCGCC 
CTCTACAAGA ACCTCCTGCA GTCCAAGGAG CCGTCCTGCA GCGACAGCAC CAACGAGTAC 
ATGGACATGA AGCCCGGTGT CTCCTACGTC GTCCCCACCA AGGCAGACAG AAGACGATCC 
GCCAGAGTCG GCTCCTACAT AGAGCGAGAC GTGACGCCCG CCGTCCTGGA GGACGACGAG 
CTGGCCCTGG ACCTGGAGGA CTTGTTGAGC TTTTCCTACC AGGTGGCCAA GGGCATGGCT 
TTCCTCGCCT CCAAGAACTG TATCCACAGA GACCTGGCCG CCAGGAACAT CCTCCTTACC 
CACGGGCGCA TCACCAAGAT CTGCGACTTC GGCCTCGCCC GAGACATCAA GAGCGACTCC 
AACTATGTGG TTAAAGGAAA CGCCCGGCTC CCCGTGAAGT GGATGGCCCC GGAGAGCATC 
TTCAACTGCG TGTACACGTT TGAGAGCGAC GTCTGGTCCT ACGGCATCTT CCTGTGGGAG 
CTGTTCTCCT TAGGCAGCAG CCCCTACCCC GGGATGCCGG TGGACTCCAA GTTCTACAAG 
ATGATCAAGG AGGGCTTCCG GATGCTGAGC CCCGAGCATG CACCTGCTGA GATGTATGAC 
ATCATGAAGA CTTGCTGGGA CGCTGACCCG CTGAAAAGGC CCACCTTCAA GCAGATCGTG 
CAGCTCATCG AGACGCAGAT CTCAGAGAGC ACCCACCACG TTTACTCCAA CGTGTGCAAC 
AGCAGCCCCA ACCCCGCGCA CCCCGCCGTG GACCACTCGG TGCGCGTCAA TTCCGTGGGC 
AGCAGTGCCT CCTCCACGCA GCCCCTGCTC GTGCATGAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315999.1
CDS	46..2910
Translation	MSKHYHAGSSQPSASPGQPAPPVIQPAQPELTVDAGSRFSLECIDPGSVRWTFESLGQLSDSEHSQWTREEAEAANTGRYTCTNAGGLSSSIYVFVRDPRQLFLVGLPLYGREDSDTLVRCPLTDPDVTNYTLKECEGKPLPKDLTFVPDPKAGITIKNVRRAYHPLCLPCAADREGTTVQSEKFILKVRAAAAIKAVPVVSVSRTSCLLREGEEFSVTCTVRDVSTAVLSAWLKEDGQRTNGQVRRHSWHQGDFHYERQETLTIGAARVADSGVFMCYANNTFGSANVTTTLKVVDKGFIRVFSVTNTTMFVNDGENVDLVVQYEAYPRPTQQQWLYMNRTSASKWEDHPESENESNIRYVSKLHLTRLKGSEAGTYTFLVSNADVNASVAFNVYVNTKPKILTSKRLVNGVLQCEAAGFPEPTIDWYFCLGTEQRCSAPVWPVAVQVQNASEQPFGKPVVQSAIDSSAFKHNGTVECRASNDAGQSSAYFNFALKGNSKGILLFNPISVIYSLTCVHACVCLYVTVYLQLDSIKMGRGKKGNAITFIFTSRPPPPTTDPVPIRPVPLAGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFICSKQEDHGEAALYKNLLQSKEPSCSDSTNEYMDMKPGVSYVVPTKADRRRSARVGSYIERDVTPAVLEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKSDSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLIETQISESTHHVYSNVCNSSPNPAHPAVDHSVRVNSVGSSASSTQPLLVHED

Target ORF information:

RefSeq Version	NM_001329070.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus KIT proto-oncogene, receptor tyrosine kinase (KIT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329070.1

ORF Insert Sequence:

ATGAGCAAGC ACTACCATGC AGGCTCTTCG CAGCCATCTG CGAGCCCAGG GCAGCCGGCT 
CCCCCCGTCA TCCAGCCAGC ACAGCCGGAG CTGACCGTGG ATGCCGGCAG CAGGTTCTCG 
CTGGAGTGCA TCGACCCGGG CTCCGTCAGG TGGACTTTCG AGAGCCTGGG TCAGCTGAGT 
GACAGCGAGC ACAGCCAATG GACGCGCGAG GAGGCGGAGG CCGCCAACAC GGGCAGGTAC 
ACCTGCACCA ACGCGGGCGG CCTGAGCAGC TCCATCTACG TGTTCGTGAG AGATCCGCGG 
CAGCTGTTCC TCGTGGGCCT GCCCCTGTAC GGCCGAGAAG ACAGTGACAC GCTGGTCCGC 
TGTCCGCTGA CCGACCCGGA CGTGACCAAC TACACCCTCA AGGAGTGCGA GGGGAAGCCT 
CTCCCCAAGG ACTTGACCTT CGTCCCGGAC CCCAAGGCCG GCATCACCAT CAAGAACGTG 
AGGCGCGCCT ACCACCCCCT GTGCCTGCCC TGCGCCGCCG ACCGCGAGGG CACAACCGTG 
CAGTCGGAAA AATTCATCCT GAAAGTGAGA GCAGCCGCAG CCATCAAGGC CGTCCCGGTG 
GTGTCCGTGT CTAGGACGAG CTGCCTGCTT AGAGAGGGGG AAGAGTTCTC AGTGACCTGC 
ACGGTCAGAG ACGTGTCCAC CGCCGTGCTG TCCGCGTGGC TGAAGGAGGA CGGCCAGCGG 
ACAAACGGCC AGGTGAGGCG CCACAGCTGG CATCAGGGCG ACTTCCACTA CGAGCGCCAG 
GAGACGCTGA CGATCGGCGC GGCGAGAGTG GCGGACTCCG GGGTGTTCAT GTGCTACGCC 
AACAACACCT TCGGGTCGGC GAATGTCACC ACGACCTTGA AAGTCGTAGA TAAAGGCTTC 
ATCCGGGTCT TCTCGGTGAC CAACACCACC ATGTTTGTCA ACGACGGAGA AAACGTGGAC 
TTGGTGGTGC AGTACGAGGC GTACCCGCGG CCCACGCAGC AGCAGTGGCT GTACATGAAC 
AGGACCTCCG CCAGCAAGTG GGAGGACCAC CCAGAGTCAG AGAATGAGAG CAACATCAGA 
TACGTGAGCA AGCTGCACCT GACCAGACTG AAGGGGAGCG AGGCCGGCAC CTACACGTTT 
CTCGTGTCCA ATGCTGATGT CAACGCTTCC GTGGCCTTTA ACGTTTACGT GAACACGAAA 
CCAAAGATCC TGACTTCCAA AAGACTGGTG AACGGCGTGC TGCAGTGTGA GGCAGCAGGA 
TTCCCGGAGC CCACCATAGA CTGGTATTTC TGTCTGGGAA CCGAGCAGAG GTGCTCGGCG 
CCGGTGTGGC CGGTGGCCGT GCAGGTCCAG AATGCGTCGG AGCAGCCCTT CGGGAAGCCG 
GTGGTCCAGA GCGCCATCGA CTCGAGCGCC TTCAAGCACA ATGGCACGGT GGAGTGCAGG 
GCGTCCAACG ACGCGGGCCA GAGCTCCGCC TACTTCAACT TTGCCCTTAA AGGTAACAGC 
AAAGGTATAC TCCTCTTTAA TCCAATTTCA GTCATATACT CACTCACGTG TGTGCATGCG 
TGTGTGTGTC TTTATGTAAC TGTATATTTA CAGCTTGACT CCATTAAGAT GGGCAGAGGA 
AAAAAGGGGA ATGCAATCAC TTTTATCTTT ACTTCCCGCC CCCCGCCCCC CACCACTGAC 
CCTGTCCCCA TCCGTCCTGT TCCTCTCGCA GGGAAGACCC TGGGCGCCGG CGCCTTCGGG 
AAGGTGGTGG AGGCCACGGC CTACGGGCTG ATCAAGTCGG ATGCGGCCAT GACCGTCGCT 
GTGAAGATGC TGAAACCGAG CGCCCACCTG ACGGAGCGGG AAGCCCTCAT GTCCGAGCTC 
AAGGTGCTGA GCTACCTGGG GAATCACATG AACATCGTGA ACCTCCTGGG AGCCTGCACC 
GTCGGAGGGC CCACCCTGGT CATTACAGAG TACTGCTGCT ACGGGGACCT CCTGAACTTC 
CTGCGGAGAA AGCGGGATTC GTTCATCTGC TCCAAGCAGG AGGACCACGG GGAAGCCGCC 
CTCTACAAGA ACCTCCTGCA GTCCAAGGAG CCGTCCTGCA GCGACAGCAC CAACGAGTAC 
ATGGACATGA AGCCCGGTGT CTCCTACGTC GTCCCCACCA AGGCAGACAG AAGACGATCC 
GCCAGAGTCG GCTCCTACAT AGAGCGAGAC GTGACGCCCG CCGTCCTGGA GGACGACGAG 
CTGGCCCTGG ACCTGGAGGA CTTGTTGAGC TTTTCCTACC AGGTGGCCAA GGGCATGGCT 
TTCCTCGCCT CCAAGAACTG TATCCACAGA GACCTGGCCG CCAGGAACAT CCTCCTTACC 
CACGGGCGCA TCACCAAGAT CTGCGACTTC GGCCTCGCCC GAGACATCAA GAGCGACTCC 
AACTATGTGG TTAAAGGAAA CGCCCGGCTC CCCGTGAAGT GGATGGCCCC GGAGAGCATC 
TTCAACTGCG TGTACACGTT TGAGAGCGAC GTCTGGTCCT ACGGCATCTT CCTGTGGGAG 
CTGTTCTCCT TAGGCAGCAG CCCCTACCCC GGGATGCCGG TGGACTCCAA GTTCTACAAG 
ATGATCAAGG AGGGCTTCCG GATGCTGAGC CCCGAGCATG CACCTGCTGA GATGTATGAC 
ATCATGAAGA CTTGCTGGGA CGCTGACCCG CTGAAAAGGC CCACCTTCAA GCAGATCGTG 
CAGCTCATCG AGACGCAGAT CTCAGAGAGC ACCCACCACG TTTACTCCAA CGTGTGCAAC 
AGCAGCCCCA ACCCCGCGCA CCCCGCCGTG GACCACTCGG TGCGCGTCAA TTCCGTGGGC 
AGCAGTGCCT CCTCCACGCA GCCCCTGCTC GTGCATGAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIT ( XM_017347545.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_017203034.1 Oryctolagus cuniculus KIT proto-oncogene receptor tyrosine kinase (KIT), mRNA.

Price & Availability↑

CloneID	OOb37161
Clone ID Related Accession (Same CDS sequence)	XM_017347545.1
Accession Version	XM_017347545.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466524800000
Organism	Oryctolagus cuniculus(rabbit)
Product	mast/stem cell growth factor receptor Kit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013683.1) and transcript sequence (GBAX01082195.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 234 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTTTTTT TCCGCCAAAG AAGCAGCTCG CACTCAACGA GATCTTGGTT TTTCTTGGCA 
GGCTCTTCGC AGCCATCTGC GAGCCCAGGG CAGCCGGCTC CCCCCGTCAT CCAGCCAGCA 
CAGCCGGAGC TGACCGTGGA TGCCGGCAGC AGGTTCTCGC TGGAGTGCAT CGACCCGGGC 
TCCGTCAGGT GGACTTTCGA GAGCCTGGGT CAGCTGAGTG ACAGCGAGCA CAGCCAATGG 
ACGCGCGAGG AGGCGGAGGC CGCCAACACG GGCAGGTACA CCTGCACCAA CGCGGGCGGC 
CTGAGCAGCT CCATCTACGT GTTCGTGAGA GATCCGCGGC AGCTGTTCCT CGTGGGCCTG 
CCCCTGTACG GCCGAGAAGA CAGTGACACG CTGGTCCGCT GTCCGCTGAC CGACCCGGAC 
GTGACCAACT ACACCCTCAA GGAGTGCGAG GGGAAGCCTC TCCCCAAGGA CTTGACCTTC 
GTCCCGGACC CCAAGGCCGG CATCACCATC AAGAACGTGA GGCGCGCCTA CCACCGGCTG 
TGCCTGCGCT GCGCCGCCGA CCGCGAGGGC ACAACCGTGC AGTCGGAAAA ATTCATCCTG 
AAAGTGAGAG CAGCCATCAA GGCCGTCCCG GTGGTGTCCG TGTCTAGGAC GAGCTGCCTG 
CTTAGAGAGG GGGAAGAGTT CTCAGTGACC TGCACGGTCA GAGACGTGTC CACCGCCGTG 
CTGTCCGCGT GGCTGAAGGA GGACGGCCAG CGGACAAACG GCCAGGTGAG GCGCCACAGC 
TGGCATCAGG GCGACTTCCA CTACGAGCGC CAGGAGACGC TGACGATCGG CGCGGCGAGA 
GTGGCGGACT CCGGGGTGTT CATGTGCTAC GCCAACAACA CCTTCGGGTC GGCGAATGTC 
ACCACGACCT TGAAAGTCGT AGATAAAGGC TTCATCCGGG TCTTCTCGGT GACCAACACC 
ACCATGTTTG TCAACGACGG AGAAAACGTG GACTTGGTGG TGCAGTACGA GGCGTACCCG 
CGGCCCACGC AGCAGCAGTG GCTGTACATG AACAGGACCT CCGCCAGCAA GTGGGAGGAC 
CACCCAGAGT CAGAGAATGA GAGCAACATC AGATACGTGA GCAAGCTGCA CCTGACCAGA 
CTGAAGGGGA GCGAGGCCGG CACCTACACG TTTCTCGTGT CCAATGCTGA TGTCAACGCT 
TCCGTGGCCT TTAACGTTTA CGTGAACACG AAACCAAAGA TCCTGACTTC CAAAAGACTG 
GTGAACGGCG TGCTGCAGTG TGAGGCAGCA GGATTCCCGG AGCCCACCAT AGACTGGTAT 
TTCTGTCTGG GAACCGAGCA GAGGTGCTCG GCGCCGGTGT GGCCGGTGGC CGTGCAGGTC 
CAGAATGCGT CGGAGCAGCC CTTCGGGAAG CCGGTGGTCC AGAGCGCCAT CGACTCGAGC 
GCCTTCAAGC ACAATGGCAC GGTGGAGTGC AGGGCGTCCA ACGACGCGGG CCAGAGCTCC 
GCCTACTTCA ACTTTGCCCT TAAAGGTAAC AGCAAAGAGC AGATCCAGGC GCACACCCTG 
TTCACACCTC TGTTGGTGGG GTTCGTGGTG ACGGCCGGCG TCATGTGCAT CATCGTCATG 
GTCCTCACCT ACAAGTACCT GCAGAAGCCC ATGTACGAGG TGCAGTGGAA GGTGGTGGAG 
GAGATCAATG GCAACAGCTA CGTGTACATC GACCCGACGC AGCTTCCCTA CGACCACAAG 
TGGGAGTTTC CCAGGAGTCG GCTGAGCTTT GGGAAGACCC TGGGCGCCGG CGCCTTCGGG 
AAGGTGGTGG AGGCCACGGC CTACGGGCTG ATCAAGTCGG ATGCGGCCAT GACCGTCGCT 
GTGAAGATGC TGAAACCGAG CGCCCACCTG ACGGAGCGGG AAGCCCTCAT GTCCGAGCTC 
AAGGTGCTGA GCTACCTGGG GAATCACATG AACATCGTGA ACCTCCTGGG AGCCTGCACC 
GTCGGAGGGC CCACCCTGGT CATTACAGAG TACTGCTGCT ACGGGGACCT CCTGAACTTC 
CTGCGGAGAA AGCGGGATTC GTTCATCTGC TCCAAGCAGG AGGACCACGG GGAAGCCGCC 
CTCTACAAGA ACCTCCTGCA GTCCAAGGAG CCGTCCTGCG ACAGCACCAA CGAGTACATG 
GACATGAAGC CCGGTGTCTC CTACGTCGTC CCCACCAAGG CAGACAGAAG ACGATCCGCC 
AGAGTCGGCT CCTACATAGA GCGAGACGTG ACGCCCGCCG TCCTGGAGGA CGACGAGCTG 
GCCCTGGACC TGGAGGACTT GTTGAGCTTT TCCTACCAGG TGGCCAAGGG CATGGCTTTC 
CTCGCCTCCA AGAACTGTAT CCACAGAGAC CTGGCCGCCA GGAACATCCT CCTTACCCAC 
GGGCGCATCA CCAAGATCTG CGACTTCGGC CTCGCCCGAG ACATCAAGAG CGACTCCAAC 
TATGTGGTTA AAGGAAACGC CCGGCTCCCC GTGAAGTGGA TGGCCCCGGA GAGCATCTTC 
AACTGCGTGT ACACGTTTGA GAGCGACGTC TGGTCCTACG GCATCTTCCT GTGGGAGCTG 
TTCTCCTTAG GCAGCAGCCC CTACCCCGGG ATGCCGGTGG ACTCCAAGTT CTACAAGATG 
ATCAAGGAGG GCTTCCGGAT GCTGAGCCCC GAGCATGCAC CTGCTGAGAT GTATGACATC 
ATGAAGACTT GCTGGGACGC TGACCCGCTG AAAAGGCCCA CCTTCAAGCA GATCGTGCAG 
CTCATCGAGA CGCAGATCTC AGAGAGCACC CACCACGTTT ACTCCAACGT GTGCAACAGC 
AGCCCCAACC CCGCGCACCC CGCCGTGGAC CACTCGGTGC GCGTCAATTC CGTGGGCAGC 
AGTGCCTCCT CCACGCAGCC CCTGCTCGTG CATGAAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017203034.1
CDS	267..3188
Translation	MLFFRQRSSSHSTRSWFFLAGSSQPSASPGQPAPPVIQPAQPELTVDAGSRFSLECIDPGSVRWTFESLGQLSDSEHSQWTREEAEAANTGRYTCTNAGGLSSSIYVFVRDPRQLFLVGLPLYGREDSDTLVRCPLTDPDVTNYTLKECEGKPLPKDLTFVPDPKAGITIKNVRRAYHRLCLRCAADREGTTVQSEKFILKVRAAIKAVPVVSVSRTSCLLREGEEFSVTCTVRDVSTAVLSAWLKEDGQRTNGQVRRHSWHQGDFHYERQETLTIGAARVADSGVFMCYANNTFGSANVTTTLKVVDKGFIRVFSVTNTTMFVNDGENVDLVVQYEAYPRPTQQQWLYMNRTSASKWEDHPESENESNIRYVSKLHLTRLKGSEAGTYTFLVSNADVNASVAFNVYVNTKPKILTSKRLVNGVLQCEAAGFPEPTIDWYFCLGTEQRCSAPVWPVAVQVQNASEQPFGKPVVQSAIDSSAFKHNGTVECRASNDAGQSSAYFNFALKGNSKEQIQAHTLFTPLLVGFVVTAGVMCIIVMVLTYKYLQKPMYEVQWKVVEEINGNSYVYIDPTQLPYDHKWEFPRSRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFICSKQEDHGEAALYKNLLQSKEPSCDSTNEYMDMKPGVSYVVPTKADRRRSARVGSYIERDVTPAVLEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKSDSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLIETQISESTHHVYSNVCNSSPNPAHPAVDHSVRVNSVGSSASSTQPLLVHED

Target ORF information:

RefSeq Version	XM_017347545.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus KIT proto-oncogene receptor tyrosine kinase (KIT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017347545.1

ORF Insert Sequence:

ATGCTTTTTT TCCGCCAAAG AAGCAGCTCG CACTCAACGA GATCTTGGTT TTTCTTGGCA 
GGCTCTTCGC AGCCATCTGC GAGCCCAGGG CAGCCGGCTC CCCCCGTCAT CCAGCCAGCA 
CAGCCGGAGC TGACCGTGGA TGCCGGCAGC AGGTTCTCGC TGGAGTGCAT CGACCCGGGC 
TCCGTCAGGT GGACTTTCGA GAGCCTGGGT CAGCTGAGTG ACAGCGAGCA CAGCCAATGG 
ACGCGCGAGG AGGCGGAGGC CGCCAACACG GGCAGGTACA CCTGCACCAA CGCGGGCGGC 
CTGAGCAGCT CCATCTACGT GTTCGTGAGA GATCCGCGGC AGCTGTTCCT CGTGGGCCTG 
CCCCTGTACG GCCGAGAAGA CAGTGACACG CTGGTCCGCT GTCCGCTGAC CGACCCGGAC 
GTGACCAACT ACACCCTCAA GGAGTGCGAG GGGAAGCCTC TCCCCAAGGA CTTGACCTTC 
GTCCCGGACC CCAAGGCCGG CATCACCATC AAGAACGTGA GGCGCGCCTA CCACCGGCTG 
TGCCTGCGCT GCGCCGCCGA CCGCGAGGGC ACAACCGTGC AGTCGGAAAA ATTCATCCTG 
AAAGTGAGAG CAGCCATCAA GGCCGTCCCG GTGGTGTCCG TGTCTAGGAC GAGCTGCCTG 
CTTAGAGAGG GGGAAGAGTT CTCAGTGACC TGCACGGTCA GAGACGTGTC CACCGCCGTG 
CTGTCCGCGT GGCTGAAGGA GGACGGCCAG CGGACAAACG GCCAGGTGAG GCGCCACAGC 
TGGCATCAGG GCGACTTCCA CTACGAGCGC CAGGAGACGC TGACGATCGG CGCGGCGAGA 
GTGGCGGACT CCGGGGTGTT CATGTGCTAC GCCAACAACA CCTTCGGGTC GGCGAATGTC 
ACCACGACCT TGAAAGTCGT AGATAAAGGC TTCATCCGGG TCTTCTCGGT GACCAACACC 
ACCATGTTTG TCAACGACGG AGAAAACGTG GACTTGGTGG TGCAGTACGA GGCGTACCCG 
CGGCCCACGC AGCAGCAGTG GCTGTACATG AACAGGACCT CCGCCAGCAA GTGGGAGGAC 
CACCCAGAGT CAGAGAATGA GAGCAACATC AGATACGTGA GCAAGCTGCA CCTGACCAGA 
CTGAAGGGGA GCGAGGCCGG CACCTACACG TTTCTCGTGT CCAATGCTGA TGTCAACGCT 
TCCGTGGCCT TTAACGTTTA CGTGAACACG AAACCAAAGA TCCTGACTTC CAAAAGACTG 
GTGAACGGCG TGCTGCAGTG TGAGGCAGCA GGATTCCCGG AGCCCACCAT AGACTGGTAT 
TTCTGTCTGG GAACCGAGCA GAGGTGCTCG GCGCCGGTGT GGCCGGTGGC CGTGCAGGTC 
CAGAATGCGT CGGAGCAGCC CTTCGGGAAG CCGGTGGTCC AGAGCGCCAT CGACTCGAGC 
GCCTTCAAGC ACAATGGCAC GGTGGAGTGC AGGGCGTCCA ACGACGCGGG CCAGAGCTCC 
GCCTACTTCA ACTTTGCCCT TAAAGGTAAC AGCAAAGAGC AGATCCAGGC GCACACCCTG 
TTCACACCTC TGTTGGTGGG GTTCGTGGTG ACGGCCGGCG TCATGTGCAT CATCGTCATG 
GTCCTCACCT ACAAGTACCT GCAGAAGCCC ATGTACGAGG TGCAGTGGAA GGTGGTGGAG 
GAGATCAATG GCAACAGCTA CGTGTACATC GACCCGACGC AGCTTCCCTA CGACCACAAG 
TGGGAGTTTC CCAGGAGTCG GCTGAGCTTT GGGAAGACCC TGGGCGCCGG CGCCTTCGGG 
AAGGTGGTGG AGGCCACGGC CTACGGGCTG ATCAAGTCGG ATGCGGCCAT GACCGTCGCT 
GTGAAGATGC TGAAACCGAG CGCCCACCTG ACGGAGCGGG AAGCCCTCAT GTCCGAGCTC 
AAGGTGCTGA GCTACCTGGG GAATCACATG AACATCGTGA ACCTCCTGGG AGCCTGCACC 
GTCGGAGGGC CCACCCTGGT CATTACAGAG TACTGCTGCT ACGGGGACCT CCTGAACTTC 
CTGCGGAGAA AGCGGGATTC GTTCATCTGC TCCAAGCAGG AGGACCACGG GGAAGCCGCC 
CTCTACAAGA ACCTCCTGCA GTCCAAGGAG CCGTCCTGCG ACAGCACCAA CGAGTACATG 
GACATGAAGC CCGGTGTCTC CTACGTCGTC CCCACCAAGG CAGACAGAAG ACGATCCGCC 
AGAGTCGGCT CCTACATAGA GCGAGACGTG ACGCCCGCCG TCCTGGAGGA CGACGAGCTG 
GCCCTGGACC TGGAGGACTT GTTGAGCTTT TCCTACCAGG TGGCCAAGGG CATGGCTTTC 
CTCGCCTCCA AGAACTGTAT CCACAGAGAC CTGGCCGCCA GGAACATCCT CCTTACCCAC 
GGGCGCATCA CCAAGATCTG CGACTTCGGC CTCGCCCGAG ACATCAAGAG CGACTCCAAC 
TATGTGGTTA AAGGAAACGC CCGGCTCCCC GTGAAGTGGA TGGCCCCGGA GAGCATCTTC 
AACTGCGTGT ACACGTTTGA GAGCGACGTC TGGTCCTACG GCATCTTCCT GTGGGAGCTG 
TTCTCCTTAG GCAGCAGCCC CTACCCCGGG ATGCCGGTGG ACTCCAAGTT CTACAAGATG 
ATCAAGGAGG GCTTCCGGAT GCTGAGCCCC GAGCATGCAC CTGCTGAGAT GTATGACATC 
ATGAAGACTT GCTGGGACGC TGACCCGCTG AAAAGGCCCA CCTTCAAGCA GATCGTGCAG 
CTCATCGAGA CGCAGATCTC AGAGAGCACC CACCACGTTT ACTCCAACGT GTGCAACAGC 
AGCCCCAACC CCGCGCACCC CGCCGTGGAC CACTCGGTGC GCGTCAATTC CGTGGGCAGC 
AGTGCCTCCT CCACGCAGCC CCTGCTCGTG CATGAAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The KIT gene is associated with the english spotting coat color locus and congenital megacolon in Checkered Giant rabbits (Oryctolagus cuniculus).
PloS one9(4)e93750(2014)
Fontanesi L,Vargiolu M,Scotti E,Latorre R,Faussone Pellegrini MS,Mazzoni M,Asti M,Chiocchetti R,Romeo G,Clavenzani P,De Giorgio R

KIT/KIT ligand in mammalian oogenesis and folliculogenesis: roles in rabbit and murine ovarian follicle activation and oocyte growth.
Biology of reproduction75(3)421-33(2006 Sep)
Hutt KJ,McLaughlin EA,Holland MK

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KIT cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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