ESPL1 cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	ESPL1
Entrez Gene ID	101105898
Full Name	extra spindle pole bodies like 1, separase
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012149250.2	XP_012004640.1	separin isoform X2
XM_012149252.2	XP_012004642.1	separin isoform X3
XM_012174193.2	XP_012029583.1	separin isoform X1
XM_027967256.1	XP_027823057.1	separin

Pathways from BioSystems
Gene Ontology

The following ESPL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ESPL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv25542	XM_012149250.2 Latest version!	Ovis aries musimon extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOv25543	XM_012149252.2 Latest version!	Ovis aries musimon extra spindle pole bodies like 1, separase (ESPL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm26796	XM_012174193.2 Latest version!	Ovis aries extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm77755	XM_027967256.1 Latest version!	Ovis aries extra spindle pole bodies like 1, separase (ESPL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ESPL1 ( XM_012149250.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012004640.1 Ovis aries musimon extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.

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CloneID	OOv25542
Clone ID Related Accession (Same CDS sequence)	XM_012149250.2
Accession Version	XM_012149250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	separin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942608.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012149250.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCT CTGTTGTGCC CTCCGGCACC ATGAGGAGCT TCAGAGGAGT CAACTTTGGG 
ACCCTGCTAA GCAGTCCAAA GGAGGCTGAA GAGCTGCTGC CTGACTTGAA GGAGTTCCTG 
ACCAAGCTTC CCAGCTGCCG GTCTGATTCC GAGAGGAGAC AAACTTGTGA TGCCATTCTG 
AGGGCTTGCA ACCAGCAGCT GACTGTCAAG CTGGCTTGCC CTAGCCACCT GGGAAGCCTT 
CTGGAGCTGG CGGAGCTGGC TTGCGATGGC TACTTGATGT CTACACCACA GCGCCCACCC 
CTCTACCTGG AACGAATTCT TTTCATCTTT CTACGGAACG TTGCTGCACA GGGAGACCCA 
GAAGCCACTC TCCGACTTGC TCAGCCCCTC CATGCCTGCT TGGTGCAATG TCCTCGCCAA 
GCTGCTCCTC AGGACTATGA GGCTGTGGCT CGTGGCAGCT TTTCTCTGCT TTGGAAGGGG 
GCAGAAGGCT TGATGGAGCG GCGAGCTGCA TTCTCAGCTC GGTTAAAGGC CTTGAGCTTT 
CTAGTACTCT TGGAGGATGA AAGTATCCCT TGTGAGATTC CCCACTTTGC TTCTCCAACA 
GCCTGTCGAG TGGTAGCTGC CCATCAGCTG TTTGAAGCCA GTGGGCATGG TCTAAATGAA 
GCAGATGCTG ATTTCCTAGA TGACCTGCTC TCCAGGCATG TGATCACAGC TTTGGTGGGT 
GAAGGAGCCT CTCCTGGTCC TCTTTCCCCC CAGAGGGCCC TCTGTCTCTT GGAGCTCACC 
CTGGAACACT GTCGTCGCCT CTGCTGGAAC CACCACCACA CCAGGGCCAC AAGTACAGTG 
GAGAAGGCCC ATGATTACCT AAGGAACACC AGTCTGGCCC CCAGCCTCCA GCTATGCCAG 
CTGGCAGTTG AACAGTTGCT GGCTGGGGAA GGAGGACCCC AGGCAGTGGC CAAGCTTCTG 
ATCAAGGCAT CAGCTATCCT GAAGAACAGC TTGGAGGCAC CATCACCCCC ACTGCGAGCA 
TTGTGTGACA GCTGCCAGTT CTTCCTCTCA GTCCTGGAGC GAGGCACCAA GAGGTGCTAT 
GGACTTGATG CCATTCTGAG CCTCTTTGCT TTTCTTGGCA GCTACTGTTC CCTCATCCAG 
AAGCTGCAGG ATGGTGTCTG TGGGGACTCC TCCAAGCAGC TGCAGGCTTT GGTTCAGATA 
CACTTTCAGG GACTTCACCT CTACACTGCG GTGGTTTATG ACTTTGCCCA AAGCTGTCAG 
GAAGCTAATT TGGCTGACCT GGCCCAGCTA GCGAACAGTT GCAGATCTAC TGGTATCTGG 
ATGCTGGAGG CCTTACAGGG CCTACCTGGC CAAGAGCTGA CTGACTACCT GGGGATGACT 
GTCTCGTACA CCAGTAACCT GGCCTACAGC TTCTACAGTC ACAAGCTCTA TGCCGAAGCC 
TGTGCCATCT CCGAGCCCCT CTGTCAGCAC CTGGGCTCAG TGAAGCCAGG CACCTATCCT 
GAGGTGCCAC CTGAGAAGTT GCACAGGTGC TTCCGGCTAC ACATAGAGAG TTTAAAGAAA 
CTGGGTAAAC AGGCCCAGGG CTGCAAGATG GTGACTTTGT GGCTGGAGGC CCTGCAGCCC 
TGTGGCCCCG AACACATGAC TGAGCCAGTC ACCTTCTGGG TTCGGGTCAA GATGGATGCT 
GCCAGAGCTG GAGACAAGGA GCTACAGCTG AAGACGCTGC GAGATAGCCT GAGTGGCTGG 
GACCCGGAGA CCCTGGCCCG CTTGCTCAGG GAGGAGCTGC AGGCCTACAA GGCAGTGCGG 
GCCAACACAG GGCAGGAACG GTTCAATGTC ATCTGTGACC TGCTGGAGCT GAGCCCCGAG 
GAGACACCAG CCGGGGCCTG GGCCCGAGCC ACCCACCTGG TGGAACTGGC TCAGGTGCTC 
TGCTATCACA ACTTTGCCCA GCAGACTGAC TGCACTGCCC TCGATGCCAT CCGGGAAGCT 
CTGCAGCTTC TGGAATCTGT GAAGCCTGAG GCCCAGGACA AGGATCGGCT TTTGGATGAT 
AAAGCCCAGG CCCTGCTGTG GCTCTACATA TGCACCATGG AGGCCAAAAT GCAGGAGGGT 
ATCGAGCGGG ATCGGAGAGC CCAGGCCCCT TGTAACCTGG AGGAGTTTGA AGCCAATGAC 
CTGAATTATG AAGATAAACT CCAGGAAGAT CGCTTCCTAT ACAGTAACAT TGCCTTCAAC 
CTGGCTGCAG ATGCTGCACA ATCGAAATGC CTGGACCAAG CCCTGGCCCT GTGGAAGGAG 
GTGCTCACTA AGGGGCAGGT GCCCGGTATA CGGTGTCTCC AGCAAACAGC AGCCTCCCTG 
CAGATCCTAG CGGCCCTCTA TCAGCTGCTG GCAAAGCCCC TGCAGGCTCT GGAGGTCCTC 
CTGCTCGTCC AGATCGTCTC ACAGAGACTG AAGGACCATG CGAAGGTAGC CGGCTCTTCC 
TGCCAAGTCA CCCAGCTCCT CCTGACCCTC GGCTGTCCCA GCTACGCCCA GGTGCACCTG 
GAAGAAGCAG AGTCGCGTCT GAAGCTTCTG GATCACACCA CAGACACATA TCTGCTCCTC 
TCCCTGACCT GTGACCTCCT GCGAAGTCGA CTCTGCTGCG CTCGCCAGAA GGTGGCTGAG 
GGTGCTGCTC TGCTGCTGTC TGTGCTTCAG GACCCTGCCC TCCAGAGGGC ATCCAAGGCC 
TGGTACTTGC TGCGCGTCCA GGCCCTGCAG CTGGTGGCGG CCTACCTGAG CCTCTCGCCA 
GACAGCCTGC TGGCCCCGCT GTGGGAGCAA CTGTGTGCCC AAGGCTGGCA GACACCTGAG 
ATAGCACTCA TCGACTCCCA TAAGCTTCTC CGAAGTATCA TCCTCCTGCT CATGGGCAGC 
GATGTGCTCT CAATTCAGAA AACAGCTGTG GAGACGCCAT TTCTGGACTA TGGTGAAAAT 
CTGGTACAAA AATGGCAGGT TCTTTCTGAG GTGCTGAGCT GCTCGGAGAA GCTGGTCTCC 
CGCCTGGGCC GCCTTGGTAA TGTGAGCGAA GCGAAAGCCT TTTGCTTGGA GGCCCTGAAA 
CTTACAACAA AGCTGCAGAT ACCACGCCAG TGTGCCCTCT TCCTGGTGCT GAAGGGGGAG 
CTGGAGCTGG CACGCAATGA CATGGACCTC TGTCAGTCGG ACCTGCATCA GGTTCTGTTC 
TTGCTCGAGT CTTGCACAGA GTTCCTCGGA CTAGCGCAGC ACCCGGACTC TGTGAAGAAG 
GTCCACCTGC AGAAGGGGAA GCAGCGGGCT CGGGTGCCCC ATCTTCCAGA TCTCCCGGAG 
GAAGAGCTCA TTCTAAAAGG CCCTGCCCTG GAGCTGGTGG CCACCGTGGC CAAGGAACCT 
GGCCCTGTAG CACCCTCTGC TAACTCCTCC CCGATCCTGA AAACCAAGCC CCAGCCCAGC 
CCCAGCTTCC TGACCCACTT GCCCACCTGT GACTGCTCGC TCTGTGCCAG CCCTGTCCTC 
TCGGTGGTCT GTCTGCGCTG GGCATTGGTC ACAGCGGGGC TGAGACTGGC CATGGGCCAT 
GAGGCCCAAG GGCTGGATCT GCTGCAGGTG GTGCTGAAGG GTTGCCCTGC AGCCGGTAGT 
CGCCTTGCCC AAGCGCTTCA GGCTTCCCTG AATCACACAG CACCCCCCTC CCCTGTCCCA 
GGCCTCTTTG ATGAGATCTT GGCTCAGGCA TATACACAGC TGGCCCTGGA GGGGCTGAGC 
CAGCCATCAA ACAAGAGCCT GGGAAAGGTC TTGGAGTCAG GGTTAACGTT TGTGGTGGCG 
CGGATACCCC ACCTGGAGCC CTGGCGAGCC AACCTGCTCT TGGTCCAGGC CCTTGCAAAG 
CTGACCAGCC TCAGCTGCTG CACTGCCCAA CTTTTTGCAA GCTCCTGGGG CTGGCAGCCG 
CCATCAATAA ATAACCCCAC AGGCTCAGAA CCCTCTAAGC CTCGGAACCA AAAACAGTCA 
GGACAGTCCA GACGAGGATG CCAGCAAGTG GGCTCTGCTA CCCTGCCCCT CTCACATACC 
TCTCTGAAAG GTCTGGAAGG TGGAGGACCA CCCTGTACAC CTAAGCCCCC AGGCCGGGTC 
AGGCAGGCTG GCCCTGGGGT ACCCTTCACC GTATTTGAGG AAGTCTTCCT TACAAAGACC 
AAGCCTGAGG TTCCCAAGGC CCCCAGGGTA CAACGGAGGG TCCAGACACG CCTCAAGGTG 
AACTTCAGTG ACGACAGTGA CTTGGAAGAC CCTGTCTCAG TTGAAGCACA GCTTGCAGAG 
GGATCCAAGA GACAGGGCAC TGCCTCCCGG GGCCGGGGCC GGGCCAGAAA GGGCCCGAGT 
GTGAAGACCA ACTCAGTGGC CACGTCAGGC AGTGCCCCCG GCCTCAGTGG CAGAAGCCGG 
AGGGTCAAGA AGGTGGCATC AGGACGTTGT GAGGAGCTGG GCCCTGAGAT GATGAGGACC 
ATCCCTGAGG AGGAACTGAC TGACAACCAG ATGGAAATGA GCTTTGAGAT CCTCAGGGGT 
TCTGATGGGG AAGATTTAGC CTCAGGTGGG AAGACACCAG CTCCAGCGCC TGATACAGCC 
GTAGGAGAAT GTGAGGTGTT GAGACGGGAT GCCAGCAAAG AGGAGCTGCC CGGCCCAGAC 
AAGGAGAGAG ACATGGATCT GGGTCCCCGG CGCAGACTCC CTTCGGCCCC CATAGCCATT 
GATCTGTCTA CCCTGGATTC CATCTGTGAC TCACTGAGTG GTGCCTTCCG TGGCATCAGT 
CACTGCCCTC CTAGTGGGCT CTATGCCCAC CTCTGCCGCC TCCTGGCCCT GTGCCTGGGC 
CACCGGGATC CCTATGCCAC CGCCTGCCTT ATCACCGAGT CTGTCTCCAT CACCTGTCGC 
CACCAGCTGC TCACCCACCT CCACAGGCAG CTCAACAAGG CCCAGAAGCG CCGAGGGCCG 
CCGGACATAG CAGACCAGTT GCAGGGGCTG CACCTCCAGG AGAAGCCTGG AGACATCCCT 
CTGGCACGAA TCCAGCGCCT CTTTTCCTTC AGGCCTCTGG GATCTGGCCA CTTCCCCCAG 
CCTGAGAAGG ACAGCTTCCG GGAGCGTCTT GCTCTGATTC CCAGTGGGGT GACCGTGTGT 
GTTTTGGCCC TGGCCACCCT CCAGCCTGGA ACTGTGGGCA ACACCCTCCT GCTGACTCGG 
CTGGAGAAAG ACAAACCCCC GGTCACTGTA CAGATCCCCA CTTCCCAGAG CAAGCTTTCT 
GTGAGTTCGG CCTTGAAAGA GTTTGACGCC ATCCAGAAGG AGCAGAAAGA GAACAGCAGC 
TGTACTGACA AGCGAGAATG GTGGACGGGC CGGCTAGAGC TGGATCGCAG GATGGAGGTT 
CTCACCACTT CCCTAGAGAA GTACGTGCTG GGCTGCTGGC GGGGGCTGTT GCTGCCATCC 
AGTGAGGACC CTGGCCTTGT CCAGGAGGCC TCCCGTCTAC AAGAGTCACT ACAGCAATGT 
GGCTGGAAAT ATCCCGACCC CACTCTGCTG AGAATCATGC TCAGCGGTGC CAGTACCCTC 
ACCCCGCAGG ACGTGCAGGC CCTGGCTTAT GGGCTGTGCC CAGCCCGGCC AGCCCGAGCC 
CAAGAGCTCC TGGGTGAGGC AGTAGGGCGT CTCCAAGGTC AGATGGCAAC AAGCAGTAGG 
CATCTCGTGT TAGTGCTGGA CAAGGACCTA CAGAAGCTGC CGTGGGAGAG CATGCCCAGC 
CTCCGGGCCC TGCCTGTCAC TCGGCTGCCC TCCTTCCGAT TCCTACTCAG CTACTCCGTC 
ATCAAAGAGT CCGGGGCCTC ATCAGTGCTG AGCCAAGGAG TGGATCCTCG AAGTACCTTC 
TACGTCCTGA ACCCTCACAA TAACCTGTCA AGCACAGAGG AGCAATTCCG AGCCCATTTT 
AGCAGTGAAG CTGGCTGGAA AGGGGTGGTT GGGGAGGTGC CAAGCCCGGA ACAGGTGCAA 
ATGGCCCTGA CGGAGCGTGA CTTGTACATC TATGCAGGGC ATGGCGCCGG TGCCCGCTTC 
CTGGATGGGC AGGCTGTCCT GCGGCTGAGC TGCCGGGCAG TGACCCTGCT GTTCGGCTGC 
AGCAGCGCAG CCCTGGCTGT GCATGGAAAC CTGGAGGGGG CTGGCATCGT GCTCAAGTAT 
ATCATGGCTG GCTGCCCCCT GTTTCTAGGT AACCTCTGGG ACGTGACTGA CCGTGACATT 
GACCGCTACA CAGAGGCTCT GTTGCAGGGC TGGCTTAGAG CAGGTTCAGG AGCCCCCCTT 
CTGTACTATG TCAGCCAGGC CCGCCAGGCT CCCCGACTCA AGTACCTTAT TGGGGCTGCA 
CCTGTAGCCT ATGGCTTGCC TGTCTCCCTG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012004640.1
CDS	225..6560
Translation	MEASVVPSGTMRSFRGVNFGTLLSSPKEAEELLPDLKEFLTKLPSCRSDSERRQTCDAILRACNQQLTVKLACPSHLGSLLELAELACDGYLMSTPQRPPLYLERILFIFLRNVAAQGDPEATLRLAQPLHACLVQCPRQAAPQDYEAVARGSFSLLWKGAEGLMERRAAFSARLKALSFLVLLEDESIPCEIPHFASPTACRVVAAHQLFEASGHGLNEADADFLDDLLSRHVITALVGEGASPGPLSPQRALCLLELTLEHCRRLCWNHHHTRATSTVEKAHDYLRNTSLAPSLQLCQLAVEQLLAGEGGPQAVAKLLIKASAILKNSLEAPSPPLRALCDSCQFFLSVLERGTKRCYGLDAILSLFAFLGSYCSLIQKLQDGVCGDSSKQLQALVQIHFQGLHLYTAVVYDFAQSCQEANLADLAQLANSCRSTGIWMLEALQGLPGQELTDYLGMTVSYTSNLAYSFYSHKLYAEACAISEPLCQHLGSVKPGTYPEVPPEKLHRCFRLHIESLKKLGKQAQGCKMVTLWLEALQPCGPEHMTEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLARLLREELQAYKAVRANTGQERFNVICDLLELSPEETPAGAWARATHLVELAQVLCYHNFAQQTDCTALDAIREALQLLESVKPEAQDKDRLLDDKAQALLWLYICTMEAKMQEGIERDRRAQAPCNLEEFEANDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKEVLTKGQVPGIRCLQQTAASLQILAALYQLLAKPLQALEVLLLVQIVSQRLKDHAKVAGSSCQVTQLLLTLGCPSYAQVHLEEAESRLKLLDHTTDTYLLLSLTCDLLRSRLCCARQKVAEGAALLLSVLQDPALQRASKAWYLLRVQALQLVAAYLSLSPDSLLAPLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDVLSIQKTAVETPFLDYGENLVQKWQVLSEVLSCSEKLVSRLGRLGNVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDMDLCQSDLHQVLFLLESCTEFLGLAQHPDSVKKVHLQKGKQRARVPHLPDLPEEELILKGPALELVATVAKEPGPVAPSANSSPILKTKPQPSPSFLTHLPTCDCSLCASPVLSVVCLRWALVTAGLRLAMGHEAQGLDLLQVVLKGCPAAGSRLAQALQASLNHTAPPSPVPGLFDEILAQAYTQLALEGLSQPSNKSLGKVLESGLTFVVARIPHLEPWRANLLLVQALAKLTSLSCCTAQLFASSWGWQPPSINNPTGSEPSKPRNQKQSGQSRRGCQQVGSATLPLSHTSLKGLEGGGPPCTPKPPGRVRQAGPGVPFTVFEEVFLTKTKPEVPKAPRVQRRVQTRLKVNFSDDSDLEDPVSVEAQLAEGSKRQGTASRGRGRARKGPSVKTNSVATSGSAPGLSGRSRRVKKVASGRCEELGPEMMRTIPEEELTDNQMEMSFEILRGSDGEDLASGGKTPAPAPDTAVGECEVLRRDASKEELPGPDKERDMDLGPRRRLPSAPIAIDLSTLDSICDSLSGAFRGISHCPPSGLYAHLCRLLALCLGHRDPYATACLITESVSITCRHQLLTHLHRQLNKAQKRRGPPDIADQLQGLHLQEKPGDIPLARIQRLFSFRPLGSGHFPQPEKDSFRERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDKPPVTVQIPTSQSKLSVSSALKEFDAIQKEQKENSSCTDKREWWTGRLELDRRMEVLTTSLEKYVLGCWRGLLLPSSEDPGLVQEASRLQESLQQCGWKYPDPTLLRIMLSGASTLTPQDVQALAYGLCPARPARAQELLGEAVGRLQGQMATSSRHLVLVLDKDLQKLPWESMPSLRALPVTRLPSFRFLLSYSVIKESGASSVLSQGVDPRSTFYVLNPHNNLSSTEEQFRAHFSSEAGWKGVVGEVPSPEQVQMALTERDLYIYAGHGAGARFLDGQAVLRLSCRAVTLLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLRAGSGAPLLYYVSQARQAPRLKYLIGAAPVAYGLPVSLQ

Target ORF information:

RefSeq Version	XM_012149250.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012149250.2

ORF Insert Sequence:

ATGGAGGCCT CTGTTGTGCC CTCCGGCACC ATGAGGAGCT TCAGAGGAGT CAACTTTGGG 
ACCCTGCTAA GCAGTCCAAA GGAGGCTGAA GAGCTGCTGC CTGACTTGAA GGAGTTCCTG 
ACCAAGCTTC CCAGCTGCCG GTCTGATTCC GAGAGGAGAC AAACTTGTGA TGCCATTCTG 
AGGGCTTGCA ACCAGCAGCT GACTGTCAAG CTGGCTTGCC CTAGCCACCT GGGAAGCCTT 
CTGGAGCTGG CGGAGCTGGC TTGCGATGGC TACTTGATGT CTACACCACA GCGCCCACCC 
CTCTACCTGG AACGAATTCT TTTCATCTTT CTACGGAACG TTGCTGCACA GGGAGACCCA 
GAAGCCACTC TCCGACTTGC TCAGCCCCTC CATGCCTGCT TGGTGCAATG TCCTCGCCAA 
GCTGCTCCTC AGGACTATGA GGCTGTGGCT CGTGGCAGCT TTTCTCTGCT TTGGAAGGGG 
GCAGAAGGCT TGATGGAGCG GCGAGCTGCA TTCTCAGCTC GGTTAAAGGC CTTGAGCTTT 
CTAGTACTCT TGGAGGATGA AAGTATCCCT TGTGAGATTC CCCACTTTGC TTCTCCAACA 
GCCTGTCGAG TGGTAGCTGC CCATCAGCTG TTTGAAGCCA GTGGGCATGG TCTAAATGAA 
GCAGATGCTG ATTTCCTAGA TGACCTGCTC TCCAGGCATG TGATCACAGC TTTGGTGGGT 
GAAGGAGCCT CTCCTGGTCC TCTTTCCCCC CAGAGGGCCC TCTGTCTCTT GGAGCTCACC 
CTGGAACACT GTCGTCGCCT CTGCTGGAAC CACCACCACA CCAGGGCCAC AAGTACAGTG 
GAGAAGGCCC ATGATTACCT AAGGAACACC AGTCTGGCCC CCAGCCTCCA GCTATGCCAG 
CTGGCAGTTG AACAGTTGCT GGCTGGGGAA GGAGGACCCC AGGCAGTGGC CAAGCTTCTG 
ATCAAGGCAT CAGCTATCCT GAAGAACAGC TTGGAGGCAC CATCACCCCC ACTGCGAGCA 
TTGTGTGACA GCTGCCAGTT CTTCCTCTCA GTCCTGGAGC GAGGCACCAA GAGGTGCTAT 
GGACTTGATG CCATTCTGAG CCTCTTTGCT TTTCTTGGCA GCTACTGTTC CCTCATCCAG 
AAGCTGCAGG ATGGTGTCTG TGGGGACTCC TCCAAGCAGC TGCAGGCTTT GGTTCAGATA 
CACTTTCAGG GACTTCACCT CTACACTGCG GTGGTTTATG ACTTTGCCCA AAGCTGTCAG 
GAAGCTAATT TGGCTGACCT GGCCCAGCTA GCGAACAGTT GCAGATCTAC TGGTATCTGG 
ATGCTGGAGG CCTTACAGGG CCTACCTGGC CAAGAGCTGA CTGACTACCT GGGGATGACT 
GTCTCGTACA CCAGTAACCT GGCCTACAGC TTCTACAGTC ACAAGCTCTA TGCCGAAGCC 
TGTGCCATCT CCGAGCCCCT CTGTCAGCAC CTGGGCTCAG TGAAGCCAGG CACCTATCCT 
GAGGTGCCAC CTGAGAAGTT GCACAGGTGC TTCCGGCTAC ACATAGAGAG TTTAAAGAAA 
CTGGGTAAAC AGGCCCAGGG CTGCAAGATG GTGACTTTGT GGCTGGAGGC CCTGCAGCCC 
TGTGGCCCCG AACACATGAC TGAGCCAGTC ACCTTCTGGG TTCGGGTCAA GATGGATGCT 
GCCAGAGCTG GAGACAAGGA GCTACAGCTG AAGACGCTGC GAGATAGCCT GAGTGGCTGG 
GACCCGGAGA CCCTGGCCCG CTTGCTCAGG GAGGAGCTGC AGGCCTACAA GGCAGTGCGG 
GCCAACACAG GGCAGGAACG GTTCAATGTC ATCTGTGACC TGCTGGAGCT GAGCCCCGAG 
GAGACACCAG CCGGGGCCTG GGCCCGAGCC ACCCACCTGG TGGAACTGGC TCAGGTGCTC 
TGCTATCACA ACTTTGCCCA GCAGACTGAC TGCACTGCCC TCGATGCCAT CCGGGAAGCT 
CTGCAGCTTC TGGAATCTGT GAAGCCTGAG GCCCAGGACA AGGATCGGCT TTTGGATGAT 
AAAGCCCAGG CCCTGCTGTG GCTCTACATA TGCACCATGG AGGCCAAAAT GCAGGAGGGT 
ATCGAGCGGG ATCGGAGAGC CCAGGCCCCT TGTAACCTGG AGGAGTTTGA AGCCAATGAC 
CTGAATTATG AAGATAAACT CCAGGAAGAT CGCTTCCTAT ACAGTAACAT TGCCTTCAAC 
CTGGCTGCAG ATGCTGCACA ATCGAAATGC CTGGACCAAG CCCTGGCCCT GTGGAAGGAG 
GTGCTCACTA AGGGGCAGGT GCCCGGTATA CGGTGTCTCC AGCAAACAGC AGCCTCCCTG 
CAGATCCTAG CGGCCCTCTA TCAGCTGCTG GCAAAGCCCC TGCAGGCTCT GGAGGTCCTC 
CTGCTCGTCC AGATCGTCTC ACAGAGACTG AAGGACCATG CGAAGGTAGC CGGCTCTTCC 
TGCCAAGTCA CCCAGCTCCT CCTGACCCTC GGCTGTCCCA GCTACGCCCA GGTGCACCTG 
GAAGAAGCAG AGTCGCGTCT GAAGCTTCTG GATCACACCA CAGACACATA TCTGCTCCTC 
TCCCTGACCT GTGACCTCCT GCGAAGTCGA CTCTGCTGCG CTCGCCAGAA GGTGGCTGAG 
GGTGCTGCTC TGCTGCTGTC TGTGCTTCAG GACCCTGCCC TCCAGAGGGC ATCCAAGGCC 
TGGTACTTGC TGCGCGTCCA GGCCCTGCAG CTGGTGGCGG CCTACCTGAG CCTCTCGCCA 
GACAGCCTGC TGGCCCCGCT GTGGGAGCAA CTGTGTGCCC AAGGCTGGCA GACACCTGAG 
ATAGCACTCA TCGACTCCCA TAAGCTTCTC CGAAGTATCA TCCTCCTGCT CATGGGCAGC 
GATGTGCTCT CAATTCAGAA AACAGCTGTG GAGACGCCAT TTCTGGACTA TGGTGAAAAT 
CTGGTACAAA AATGGCAGGT TCTTTCTGAG GTGCTGAGCT GCTCGGAGAA GCTGGTCTCC 
CGCCTGGGCC GCCTTGGTAA TGTGAGCGAA GCGAAAGCCT TTTGCTTGGA GGCCCTGAAA 
CTTACAACAA AGCTGCAGAT ACCACGCCAG TGTGCCCTCT TCCTGGTGCT GAAGGGGGAG 
CTGGAGCTGG CACGCAATGA CATGGACCTC TGTCAGTCGG ACCTGCATCA GGTTCTGTTC 
TTGCTCGAGT CTTGCACAGA GTTCCTCGGA CTAGCGCAGC ACCCGGACTC TGTGAAGAAG 
GTCCACCTGC AGAAGGGGAA GCAGCGGGCT CGGGTGCCCC ATCTTCCAGA TCTCCCGGAG 
GAAGAGCTCA TTCTAAAAGG CCCTGCCCTG GAGCTGGTGG CCACCGTGGC CAAGGAACCT 
GGCCCTGTAG CACCCTCTGC TAACTCCTCC CCGATCCTGA AAACCAAGCC CCAGCCCAGC 
CCCAGCTTCC TGACCCACTT GCCCACCTGT GACTGCTCGC TCTGTGCCAG CCCTGTCCTC 
TCGGTGGTCT GTCTGCGCTG GGCATTGGTC ACAGCGGGGC TGAGACTGGC CATGGGCCAT 
GAGGCCCAAG GGCTGGATCT GCTGCAGGTG GTGCTGAAGG GTTGCCCTGC AGCCGGTAGT 
CGCCTTGCCC AAGCGCTTCA GGCTTCCCTG AATCACACAG CACCCCCCTC CCCTGTCCCA 
GGCCTCTTTG ATGAGATCTT GGCTCAGGCA TATACACAGC TGGCCCTGGA GGGGCTGAGC 
CAGCCATCAA ACAAGAGCCT GGGAAAGGTC TTGGAGTCAG GGTTAACGTT TGTGGTGGCG 
CGGATACCCC ACCTGGAGCC CTGGCGAGCC AACCTGCTCT TGGTCCAGGC CCTTGCAAAG 
CTGACCAGCC TCAGCTGCTG CACTGCCCAA CTTTTTGCAA GCTCCTGGGG CTGGCAGCCG 
CCATCAATAA ATAACCCCAC AGGCTCAGAA CCCTCTAAGC CTCGGAACCA AAAACAGTCA 
GGACAGTCCA GACGAGGATG CCAGCAAGTG GGCTCTGCTA CCCTGCCCCT CTCACATACC 
TCTCTGAAAG GTCTGGAAGG TGGAGGACCA CCCTGTACAC CTAAGCCCCC AGGCCGGGTC 
AGGCAGGCTG GCCCTGGGGT ACCCTTCACC GTATTTGAGG AAGTCTTCCT TACAAAGACC 
AAGCCTGAGG TTCCCAAGGC CCCCAGGGTA CAACGGAGGG TCCAGACACG CCTCAAGGTG 
AACTTCAGTG ACGACAGTGA CTTGGAAGAC CCTGTCTCAG TTGAAGCACA GCTTGCAGAG 
GGATCCAAGA GACAGGGCAC TGCCTCCCGG GGCCGGGGCC GGGCCAGAAA GGGCCCGAGT 
GTGAAGACCA ACTCAGTGGC CACGTCAGGC AGTGCCCCCG GCCTCAGTGG CAGAAGCCGG 
AGGGTCAAGA AGGTGGCATC AGGACGTTGT GAGGAGCTGG GCCCTGAGAT GATGAGGACC 
ATCCCTGAGG AGGAACTGAC TGACAACCAG ATGGAAATGA GCTTTGAGAT CCTCAGGGGT 
TCTGATGGGG AAGATTTAGC CTCAGGTGGG AAGACACCAG CTCCAGCGCC TGATACAGCC 
GTAGGAGAAT GTGAGGTGTT GAGACGGGAT GCCAGCAAAG AGGAGCTGCC CGGCCCAGAC 
AAGGAGAGAG ACATGGATCT GGGTCCCCGG CGCAGACTCC CTTCGGCCCC CATAGCCATT 
GATCTGTCTA CCCTGGATTC CATCTGTGAC TCACTGAGTG GTGCCTTCCG TGGCATCAGT 
CACTGCCCTC CTAGTGGGCT CTATGCCCAC CTCTGCCGCC TCCTGGCCCT GTGCCTGGGC 
CACCGGGATC CCTATGCCAC CGCCTGCCTT ATCACCGAGT CTGTCTCCAT CACCTGTCGC 
CACCAGCTGC TCACCCACCT CCACAGGCAG CTCAACAAGG CCCAGAAGCG CCGAGGGCCG 
CCGGACATAG CAGACCAGTT GCAGGGGCTG CACCTCCAGG AGAAGCCTGG AGACATCCCT 
CTGGCACGAA TCCAGCGCCT CTTTTCCTTC AGGCCTCTGG GATCTGGCCA CTTCCCCCAG 
CCTGAGAAGG ACAGCTTCCG GGAGCGTCTT GCTCTGATTC CCAGTGGGGT GACCGTGTGT 
GTTTTGGCCC TGGCCACCCT CCAGCCTGGA ACTGTGGGCA ACACCCTCCT GCTGACTCGG 
CTGGAGAAAG ACAAACCCCC GGTCACTGTA CAGATCCCCA CTTCCCAGAG CAAGCTTTCT 
GTGAGTTCGG CCTTGAAAGA GTTTGACGCC ATCCAGAAGG AGCAGAAAGA GAACAGCAGC 
TGTACTGACA AGCGAGAATG GTGGACGGGC CGGCTAGAGC TGGATCGCAG GATGGAGGTT 
CTCACCACTT CCCTAGAGAA GTACGTGCTG GGCTGCTGGC GGGGGCTGTT GCTGCCATCC 
AGTGAGGACC CTGGCCTTGT CCAGGAGGCC TCCCGTCTAC AAGAGTCACT ACAGCAATGT 
GGCTGGAAAT ATCCCGACCC CACTCTGCTG AGAATCATGC TCAGCGGTGC CAGTACCCTC 
ACCCCGCAGG ACGTGCAGGC CCTGGCTTAT GGGCTGTGCC CAGCCCGGCC AGCCCGAGCC 
CAAGAGCTCC TGGGTGAGGC AGTAGGGCGT CTCCAAGGTC AGATGGCAAC AAGCAGTAGG 
CATCTCGTGT TAGTGCTGGA CAAGGACCTA CAGAAGCTGC CGTGGGAGAG CATGCCCAGC 
CTCCGGGCCC TGCCTGTCAC TCGGCTGCCC TCCTTCCGAT TCCTACTCAG CTACTCCGTC 
ATCAAAGAGT CCGGGGCCTC ATCAGTGCTG AGCCAAGGAG TGGATCCTCG AAGTACCTTC 
TACGTCCTGA ACCCTCACAA TAACCTGTCA AGCACAGAGG AGCAATTCCG AGCCCATTTT 
AGCAGTGAAG CTGGCTGGAA AGGGGTGGTT GGGGAGGTGC CAAGCCCGGA ACAGGTGCAA 
ATGGCCCTGA CGGAGCGTGA CTTGTACATC TATGCAGGGC ATGGCGCCGG TGCCCGCTTC 
CTGGATGGGC AGGCTGTCCT GCGGCTGAGC TGCCGGGCAG TGACCCTGCT GTTCGGCTGC 
AGCAGCGCAG CCCTGGCTGT GCATGGAAAC CTGGAGGGGG CTGGCATCGT GCTCAAGTAT 
ATCATGGCTG GCTGCCCCCT GTTTCTAGGT AACCTCTGGG ACGTGACTGA CCGTGACATT 
GACCGCTACA CAGAGGCTCT GTTGCAGGGC TGGCTTAGAG CAGGTTCAGG AGCCCCCCTT 
CTGTACTATG TCAGCCAGGC CCGCCAGGCT CCCCGACTCA AGTACCTTAT TGGGGCTGCA 
CCTGTAGCCT ATGGCTTGCC TGTCTCCCTG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_012149252.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012004642.1 Ovis aries musimon extra spindle pole bodies like 1, separase (ESPL1), transcript variant X3, mRNA.

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CloneID	OOv25543
Clone ID Related Accession (Same CDS sequence)	XM_012149252.2
Accession Version	XM_012149252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	separin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942608.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012149252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAGAGGAGT CAACTTTGGG ACCCTGCTAA GCAGTCCAAA GGAGGCTGAA 
GAGCTGCTGC CTGACTTGAA GGAGTTCCTG ACCAAGCTTC CCAGCTGCCG GTCTGATTCC 
GAGAGGAGAC AAACTTGTGA TGCCATTCTG AGGGCTTGCA ACCAGCAGCT GACTGTCAAG 
CTGGCTTGCC CTAGCCACCT GGGAAGCCTT CTGGAGCTGG CGGAGCTGGC TTGCGATGGC 
TACTTGATGT CTACACCACA GCGCCCACCC CTCTACCTGG AACGAATTCT TTTCATCTTT 
CTACGGAACG TTGCTGCACA GGGAGACCCA GAAGCCACTC TCCGACTTGC TCAGCCCCTC 
CATGCCTGCT TGGTGCAATG TCCTCGCCAA GCTGCTCCTC AGGACTATGA GGCTGTGGCT 
CGTGGCAGCT TTTCTCTGCT TTGGAAGGGG GCAGAAGGCT TGATGGAGCG GCGAGCTGCA 
TTCTCAGCTC GGTTAAAGGC CTTGAGCTTT CTAGTACTCT TGGAGGATGA AAGTATCCCT 
TGTGAGATTC CCCACTTTGC TTCTCCAACA GCCTGTCGAG TGGTAGCTGC CCATCAGCTG 
TTTGAAGCCA GTGGGCATGG TCTAAATGAA GCAGATGCTG ATTTCCTAGA TGACCTGCTC 
TCCAGGCATG TGATCACAGC TTTGGTGGGT GAAGGAGCCT CTCCTGGTCC TCTTTCCCCC 
CAGAGGGCCC TCTGTCTCTT GGAGCTCACC CTGGAACACT GTCGTCGCCT CTGCTGGAAC 
CACCACCACA CCAGGGCCAC AAGTACAGTG GAGAAGGCCC ATGATTACCT AAGGAACACC 
AGTCTGGCCC CCAGCCTCCA GCTATGCCAG CTGGCAGTTG AACAGTTGCT GGCTGGGGAA 
GGAGGACCCC AGGCAGTGGC CAAGCTTCTG ATCAAGGCAT CAGCTATCCT GAAGAACAGC 
TTGGAGGCAC CATCACCCCC ACTGCGAGCA TTGTGTGACA GCTGCCAGTT CTTCCTCTCA 
GTCCTGGAGC GAGGCACCAA GAGGTGCTAT GGACTTGATG CCATTCTGAG CCTCTTTGCT 
TTTCTTGGCA GCTACTGTTC CCTCATCCAG AAGCTGCAGG ATGGTGTCTG TGGGGACTCC 
TCCAAGCAGC TGCAGGCTTT GGTTCAGATA CACTTTCAGG GACTTCACCT CTACACTGCG 
GTGGTTTATG ACTTTGCCCA AAGCTGTCAG GAAGCTAATT TGGCTGACCT GGCCCAGCTA 
GCGAACAGTT GCAGATCTAC TGGTATCTGG ATGCTGGAGG CCTTACAGGG CCTACCTGGC 
CAAGAGCTGA CTGACTACCT GGGGATGACT GTCTCGTACA CCAGTAACCT GGCCTACAGC 
TTCTACAGTC ACAAGCTCTA TGCCGAAGCC TGTGCCATCT CCGAGCCCCT CTGTCAGCAC 
CTGGGCTCAG TGAAGCCAGG CACCTATCCT GAGGTGCCAC CTGAGAAGTT GCACAGGTGC 
TTCCGGCTAC ACATAGAGAG TTTAAAGAAA CTGGGTAAAC AGGCCCAGGG CTGCAAGATG 
GTGACTTTGT GGCTGGAGGC CCTGCAGCCC TGTGGCCCCG AACACATGAC TGAGCCAGTC 
ACCTTCTGGG TTCGGGTCAA GATGGATGCT GCCAGAGCTG GAGACAAGGA GCTACAGCTG 
AAGACGCTGC GAGATAGCCT GAGTGGCTGG GACCCGGAGA CCCTGGCCCG CTTGCTCAGG 
GAGGAGCTGC AGGCCTACAA GGCAGTGCGG GCCAACACAG GGCAGGAACG GTTCAATGTC 
ATCTGTGACC TGCTGGAGCT GAGCCCCGAG GAGACACCAG CCGGGGCCTG GGCCCGAGCC 
ACCCACCTGG TGGAACTGGC TCAGGTGCTC TGCTATCACA ACTTTGCCCA GCAGACTGAC 
TGCACTGCCC TCGATGCCAT CCGGGAAGCT CTGCAGCTTC TGGAATCTGT GAAGCCTGAG 
GCCCAGGACA AGGATCGGCT TTTGGATGAT AAAGCCCAGG CCCTGCTGTG GCTCTACATA 
TGCACCATGG AGGCCAAAAT GCAGGAGGGT ATCGAGCGGG ATCGGAGAGC CCAGGCCCCT 
TGTAACCTGG AGGAGTTTGA AGCCAATGAC CTGAATTATG AAGATAAACT CCAGGAAGAT 
CGCTTCCTAT ACAGTAACAT TGCCTTCAAC CTGGCTGCAG ATGCTGCACA ATCGAAATGC 
CTGGACCAAG CCCTGGCCCT GTGGAAGGAG GTGCTCACTA AGGGGCAGGT GCCCGGTATA 
CGGTGTCTCC AGCAAACAGC AGCCTCCCTG CAGATCCTAG CGGCCCTCTA TCAGCTGCTG 
GCAAAGCCCC TGCAGGCTCT GGAGGTCCTC CTGCTCGTCC AGATCGTCTC ACAGAGACTG 
AAGGACCATG CGAAGGTAGC CGGCTCTTCC TGCCAAGTCA CCCAGCTCCT CCTGACCCTC 
GGCTGTCCCA GCTACGCCCA GGTGCACCTG GAAGAAGCAG AGTCGCGTCT GAAGCTTCTG 
GATCACACCA CAGACACATA TCTGCTCCTC TCCCTGACCT GTGACCTCCT GCGAAGTCGA 
CTCTGCTGCG CTCGCCAGAA GGTGGCTGAG GGTGCTGCTC TGCTGCTGTC TGTGCTTCAG 
GACCCTGCCC TCCAGAGGGC ATCCAAGGCC TGGTACTTGC TGCGCGTCCA GGCCCTGCAG 
CTGGTGGCGG CCTACCTGAG CCTCTCGCCA GACAGCCTGC TGGCCCCGCT GTGGGAGCAA 
CTGTGTGCCC AAGGCTGGCA GACACCTGAG ATAGCACTCA TCGACTCCCA TAAGCTTCTC 
CGAAGTATCA TCCTCCTGCT CATGGGCAGC GATGTGCTCT CAATTCAGAA AACAGCTGTG 
GAGACGCCAT TTCTGGACTA TGGTGAAAAT CTGGTACAAA AATGGCAGGT TCTTTCTGAG 
GTGCTGAGCT GCTCGGAGAA GCTGGTCTCC CGCCTGGGCC GCCTTGGTAA TGTGAGCGAA 
GCGAAAGCCT TTTGCTTGGA GGCCCTGAAA CTTACAACAA AGCTGCAGAT ACCACGCCAG 
TGTGCCCTCT TCCTGGTGCT GAAGGGGGAG CTGGAGCTGG CACGCAATGA CATGGACCTC 
TGTCAGTCGG ACCTGCATCA GGTTCTGTTC TTGCTCGAGT CTTGCACAGA GTTCCTCGGA 
CTAGCGCAGC ACCCGGACTC TGTGAAGAAG GTCCACCTGC AGAAGGGGAA GCAGCGGGCT 
CGGGTGCCCC ATCTTCCAGA TCTCCCGGAG GAAGAGCTCA TTCTAAAAGG CCCTGCCCTG 
GAGCTGGTGG CCACCGTGGC CAAGGAACCT GGCCCTGTAG CACCCTCTGC TAACTCCTCC 
CCGATCCTGA AAACCAAGCC CCAGCCCAGC CCCAGCTTCC TGACCCACTT GCCCACCTGT 
GACTGCTCGC TCTGTGCCAG CCCTGTCCTC TCGGTGGTCT GTCTGCGCTG GGCATTGGTC 
ACAGCGGGGC TGAGACTGGC CATGGGCCAT GAGGCCCAAG GGCTGGATCT GCTGCAGGTG 
GTGCTGAAGG GTTGCCCTGC AGCCGGTAGT CGCCTTGCCC AAGCGCTTCA GGCTTCCCTG 
AATCACACAG CACCCCCCTC CCCTGTCCCA GGCCTCTTTG ATGAGATCTT GGCTCAGGCA 
TATACACAGC TGGCCCTGGA GGGGCTGAGC CAGCCATCAA ACAAGAGCCT GGGAAAGGTC 
TTGGAGTCAG GGTTAACGTT TGTGGTGGCG CGGATACCCC ACCTGGAGCC CTGGCGAGCC 
AACCTGCTCT TGGTCCAGGC CCTTGCAAAG CTGACCAGCC TCAGCTGCTG CACTGCCCAA 
CTTTTTGCAA GCTCCTGGGG CTGGCAGCCG CCATCAATAA ATAACCCCAC AGGCTCAGAA 
CCCTCTAAGC CTCGGAACCA AAAACAGTCA GGACAGTCCA GACGAGGATG CCAGCAAGTG 
GGCTCTGCTA CCCTGCCCCT CTCACATACC TCTCTGAAAG GTCTGGAAGG TGGAGGACCA 
CCCTGTACAC CTAAGCCCCC AGGCCGGGTC AGGCAGGCTG GCCCTGGGGT ACCCTTCACC 
GTATTTGAGG AAGTCTTCCT TACAAAGACC AAGCCTGAGG TTCCCAAGGC CCCCAGGGTA 
CAACGGAGGG TCCAGACACG CCTCAAGGTG AACTTCAGTG ACGACAGTGA CTTGGAAGAC 
CCTGTCTCAG TTGAAGCACA GCTTGCAGAG GGATCCAAGA GACAGGGCAC TGCCTCCCGG 
GGCCGGGGCC GGGCCAGAAA GGGCCCGAGT GTGAAGACCA ACTCAGTGGC CACGTCAGGC 
AGTGCCCCCG GCCTCAGTGG CAGAAGCCGG AGGGTCAAGA AGGTGGCATC AGGACGTTGT 
GAGGAGCTGG GCCCTGAGAT GATGAGGACC ATCCCTGAGG AGGAACTGAC TGACAACCAG 
ATGGAAATGA GCTTTGAGAT CCTCAGGGGT TCTGATGGGG AAGATTTAGC CTCAGGTGGG 
AAGACACCAG CTCCAGCGCC TGATACAGCC GTAGGAGAAT GTGAGGTGTT GAGACGGGAT 
GCCAGCAAAG AGGAGCTGCC CGGCCCAGAC AAGGAGAGAG ACATGGATCT GGGTCCCCGG 
CGCAGACTCC CTTCGGCCCC CATAGCCATT GATCTGTCTA CCCTGGATTC CATCTGTGAC 
TCACTGAGTG GTGCCTTCCG TGGCATCAGT CACTGCCCTC CTAGTGGGCT CTATGCCCAC 
CTCTGCCGCC TCCTGGCCCT GTGCCTGGGC CACCGGGATC CCTATGCCAC CGCCTGCCTT 
ATCACCGAGT CTGTCTCCAT CACCTGTCGC CACCAGCTGC TCACCCACCT CCACAGGCAG 
CTCAACAAGG CCCAGAAGCG CCGAGGGCCG CCGGACATAG CAGACCAGTT GCAGGGGCTG 
CACCTCCAGG AGAAGCCTGG AGACATCCCT CTGGCACGAA TCCAGCGCCT CTTTTCCTTC 
AGGCCTCTGG GATCTGGCCA CTTCCCCCAG CCTGAGAAGG ACAGCTTCCG GGAGCGTCTT 
GCTCTGATTC CCAGTGGGGT GACCGTGTGT GTTTTGGCCC TGGCCACCCT CCAGCCTGGA 
ACTGTGGGCA ACACCCTCCT GCTGACTCGG CTGGAGAAAG ACAAACCCCC GGTCACTGTA 
CAGATCCCCA CTTCCCAGAG CAAGCTTTCT GTGAGTTCGG CCTTGAAAGA GTTTGACGCC 
ATCCAGAAGG AGCAGAAAGA GAACAGCAGC TGTACTGACA AGCGAGAATG GTGGACGGGC 
CGGCTAGAGC TGGATCGCAG GATGGAGGTT CTCACCACTT CCCTAGAGAA GTACGTGCTG 
GGCTGCTGGC GGGGGCTGTT GCTGCCATCC AGTGAGGACC CTGGCCTTGT CCAGGAGGCC 
TCCCGTCTAC AAGAGTCACT ACAGCAATGT GGCTGGAAAT ATCCCGACCC CACTCTGCTG 
AGAATCATGC TCAGCGGTGC CAGTACCCTC ACCCCGCAGG ACGTGCAGGC CCTGGCTTAT 
GGGCTGTGCC CAGCCCGGCC AGCCCGAGCC CAAGAGCTCC TGGGTGAGGC AGTAGGGCGT 
CTCCAAGGTC AGATGGCAAC AAGCAGTAGG CATCTCGTGT TAGTGCTGGA CAAGGACCTA 
CAGAAGCTGC CGTGGGAGAG CATGCCCAGC CTCCGGGCCC TGCCTGTCAC TCGGCTGCCC 
TCCTTCCGAT TCCTACTCAG CTACTCCGTC ATCAAAGAGT CCGGGGCCTC ATCAGTGCTG 
AGCCAAGGAG TGGATCCTCG AAGTACCTTC TACGTCCTGA ACCCTCACAA TAACCTGTCA 
AGCACAGAGG AGCAATTCCG AGCCCATTTT AGCAGTGAAG CTGGCTGGAA AGGGGTGGTT 
GGGGAGGTGC CAAGCCCGGA ACAGGTGCAA ATGGCCCTGA CGGAGCGTGA CTTGTACATC 
TATGCAGGGC ATGGCGCCGG TGCCCGCTTC CTGGATGGGC AGGCTGTCCT GCGGCTGAGC 
TGCCGGGCAG TGACCCTGCT GTTCGGCTGC AGCAGCGCAG CCCTGGCTGT GCATGGAAAC 
CTGGAGGGGG CTGGCATCGT GCTCAAGTAT ATCATGGCTG GCTGCCCCCT GTTTCTAGGT 
AACCTCTGGG ACGTGACTGA CCGTGACATT GACCGCTACA CAGAGGCTCT GTTGCAGGGC 
TGGCTTAGAG CAGGTTCAGG AGCCCCCCTT CTGTACTATG TCAGCCAGGC CCGCCAGGCT 
CCCCGACTCA AGTACCTTAT TGGGGCTGCA CCTGTAGCCT ATGGCTTGCC TGTCTCCCTG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012004642.1
CDS	106..6411
Translation	MRSFRGVNFGTLLSSPKEAEELLPDLKEFLTKLPSCRSDSERRQTCDAILRACNQQLTVKLACPSHLGSLLELAELACDGYLMSTPQRPPLYLERILFIFLRNVAAQGDPEATLRLAQPLHACLVQCPRQAAPQDYEAVARGSFSLLWKGAEGLMERRAAFSARLKALSFLVLLEDESIPCEIPHFASPTACRVVAAHQLFEASGHGLNEADADFLDDLLSRHVITALVGEGASPGPLSPQRALCLLELTLEHCRRLCWNHHHTRATSTVEKAHDYLRNTSLAPSLQLCQLAVEQLLAGEGGPQAVAKLLIKASAILKNSLEAPSPPLRALCDSCQFFLSVLERGTKRCYGLDAILSLFAFLGSYCSLIQKLQDGVCGDSSKQLQALVQIHFQGLHLYTAVVYDFAQSCQEANLADLAQLANSCRSTGIWMLEALQGLPGQELTDYLGMTVSYTSNLAYSFYSHKLYAEACAISEPLCQHLGSVKPGTYPEVPPEKLHRCFRLHIESLKKLGKQAQGCKMVTLWLEALQPCGPEHMTEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLARLLREELQAYKAVRANTGQERFNVICDLLELSPEETPAGAWARATHLVELAQVLCYHNFAQQTDCTALDAIREALQLLESVKPEAQDKDRLLDDKAQALLWLYICTMEAKMQEGIERDRRAQAPCNLEEFEANDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKEVLTKGQVPGIRCLQQTAASLQILAALYQLLAKPLQALEVLLLVQIVSQRLKDHAKVAGSSCQVTQLLLTLGCPSYAQVHLEEAESRLKLLDHTTDTYLLLSLTCDLLRSRLCCARQKVAEGAALLLSVLQDPALQRASKAWYLLRVQALQLVAAYLSLSPDSLLAPLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDVLSIQKTAVETPFLDYGENLVQKWQVLSEVLSCSEKLVSRLGRLGNVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDMDLCQSDLHQVLFLLESCTEFLGLAQHPDSVKKVHLQKGKQRARVPHLPDLPEEELILKGPALELVATVAKEPGPVAPSANSSPILKTKPQPSPSFLTHLPTCDCSLCASPVLSVVCLRWALVTAGLRLAMGHEAQGLDLLQVVLKGCPAAGSRLAQALQASLNHTAPPSPVPGLFDEILAQAYTQLALEGLSQPSNKSLGKVLESGLTFVVARIPHLEPWRANLLLVQALAKLTSLSCCTAQLFASSWGWQPPSINNPTGSEPSKPRNQKQSGQSRRGCQQVGSATLPLSHTSLKGLEGGGPPCTPKPPGRVRQAGPGVPFTVFEEVFLTKTKPEVPKAPRVQRRVQTRLKVNFSDDSDLEDPVSVEAQLAEGSKRQGTASRGRGRARKGPSVKTNSVATSGSAPGLSGRSRRVKKVASGRCEELGPEMMRTIPEEELTDNQMEMSFEILRGSDGEDLASGGKTPAPAPDTAVGECEVLRRDASKEELPGPDKERDMDLGPRRRLPSAPIAIDLSTLDSICDSLSGAFRGISHCPPSGLYAHLCRLLALCLGHRDPYATACLITESVSITCRHQLLTHLHRQLNKAQKRRGPPDIADQLQGLHLQEKPGDIPLARIQRLFSFRPLGSGHFPQPEKDSFRERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDKPPVTVQIPTSQSKLSVSSALKEFDAIQKEQKENSSCTDKREWWTGRLELDRRMEVLTTSLEKYVLGCWRGLLLPSSEDPGLVQEASRLQESLQQCGWKYPDPTLLRIMLSGASTLTPQDVQALAYGLCPARPARAQELLGEAVGRLQGQMATSSRHLVLVLDKDLQKLPWESMPSLRALPVTRLPSFRFLLSYSVIKESGASSVLSQGVDPRSTFYVLNPHNNLSSTEEQFRAHFSSEAGWKGVVGEVPSPEQVQMALTERDLYIYAGHGAGARFLDGQAVLRLSCRAVTLLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLRAGSGAPLLYYVSQARQAPRLKYLIGAAPVAYGLPVSLQ

Target ORF information:

RefSeq Version	XM_012149252.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon extra spindle pole bodies like 1, separase (ESPL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012149252.2

ORF Insert Sequence:

ATGAGGAGCT TCAGAGGAGT CAACTTTGGG ACCCTGCTAA GCAGTCCAAA GGAGGCTGAA 
GAGCTGCTGC CTGACTTGAA GGAGTTCCTG ACCAAGCTTC CCAGCTGCCG GTCTGATTCC 
GAGAGGAGAC AAACTTGTGA TGCCATTCTG AGGGCTTGCA ACCAGCAGCT GACTGTCAAG 
CTGGCTTGCC CTAGCCACCT GGGAAGCCTT CTGGAGCTGG CGGAGCTGGC TTGCGATGGC 
TACTTGATGT CTACACCACA GCGCCCACCC CTCTACCTGG AACGAATTCT TTTCATCTTT 
CTACGGAACG TTGCTGCACA GGGAGACCCA GAAGCCACTC TCCGACTTGC TCAGCCCCTC 
CATGCCTGCT TGGTGCAATG TCCTCGCCAA GCTGCTCCTC AGGACTATGA GGCTGTGGCT 
CGTGGCAGCT TTTCTCTGCT TTGGAAGGGG GCAGAAGGCT TGATGGAGCG GCGAGCTGCA 
TTCTCAGCTC GGTTAAAGGC CTTGAGCTTT CTAGTACTCT TGGAGGATGA AAGTATCCCT 
TGTGAGATTC CCCACTTTGC TTCTCCAACA GCCTGTCGAG TGGTAGCTGC CCATCAGCTG 
TTTGAAGCCA GTGGGCATGG TCTAAATGAA GCAGATGCTG ATTTCCTAGA TGACCTGCTC 
TCCAGGCATG TGATCACAGC TTTGGTGGGT GAAGGAGCCT CTCCTGGTCC TCTTTCCCCC 
CAGAGGGCCC TCTGTCTCTT GGAGCTCACC CTGGAACACT GTCGTCGCCT CTGCTGGAAC 
CACCACCACA CCAGGGCCAC AAGTACAGTG GAGAAGGCCC ATGATTACCT AAGGAACACC 
AGTCTGGCCC CCAGCCTCCA GCTATGCCAG CTGGCAGTTG AACAGTTGCT GGCTGGGGAA 
GGAGGACCCC AGGCAGTGGC CAAGCTTCTG ATCAAGGCAT CAGCTATCCT GAAGAACAGC 
TTGGAGGCAC CATCACCCCC ACTGCGAGCA TTGTGTGACA GCTGCCAGTT CTTCCTCTCA 
GTCCTGGAGC GAGGCACCAA GAGGTGCTAT GGACTTGATG CCATTCTGAG CCTCTTTGCT 
TTTCTTGGCA GCTACTGTTC CCTCATCCAG AAGCTGCAGG ATGGTGTCTG TGGGGACTCC 
TCCAAGCAGC TGCAGGCTTT GGTTCAGATA CACTTTCAGG GACTTCACCT CTACACTGCG 
GTGGTTTATG ACTTTGCCCA AAGCTGTCAG GAAGCTAATT TGGCTGACCT GGCCCAGCTA 
GCGAACAGTT GCAGATCTAC TGGTATCTGG ATGCTGGAGG CCTTACAGGG CCTACCTGGC 
CAAGAGCTGA CTGACTACCT GGGGATGACT GTCTCGTACA CCAGTAACCT GGCCTACAGC 
TTCTACAGTC ACAAGCTCTA TGCCGAAGCC TGTGCCATCT CCGAGCCCCT CTGTCAGCAC 
CTGGGCTCAG TGAAGCCAGG CACCTATCCT GAGGTGCCAC CTGAGAAGTT GCACAGGTGC 
TTCCGGCTAC ACATAGAGAG TTTAAAGAAA CTGGGTAAAC AGGCCCAGGG CTGCAAGATG 
GTGACTTTGT GGCTGGAGGC CCTGCAGCCC TGTGGCCCCG AACACATGAC TGAGCCAGTC 
ACCTTCTGGG TTCGGGTCAA GATGGATGCT GCCAGAGCTG GAGACAAGGA GCTACAGCTG 
AAGACGCTGC GAGATAGCCT GAGTGGCTGG GACCCGGAGA CCCTGGCCCG CTTGCTCAGG 
GAGGAGCTGC AGGCCTACAA GGCAGTGCGG GCCAACACAG GGCAGGAACG GTTCAATGTC 
ATCTGTGACC TGCTGGAGCT GAGCCCCGAG GAGACACCAG CCGGGGCCTG GGCCCGAGCC 
ACCCACCTGG TGGAACTGGC TCAGGTGCTC TGCTATCACA ACTTTGCCCA GCAGACTGAC 
TGCACTGCCC TCGATGCCAT CCGGGAAGCT CTGCAGCTTC TGGAATCTGT GAAGCCTGAG 
GCCCAGGACA AGGATCGGCT TTTGGATGAT AAAGCCCAGG CCCTGCTGTG GCTCTACATA 
TGCACCATGG AGGCCAAAAT GCAGGAGGGT ATCGAGCGGG ATCGGAGAGC CCAGGCCCCT 
TGTAACCTGG AGGAGTTTGA AGCCAATGAC CTGAATTATG AAGATAAACT CCAGGAAGAT 
CGCTTCCTAT ACAGTAACAT TGCCTTCAAC CTGGCTGCAG ATGCTGCACA ATCGAAATGC 
CTGGACCAAG CCCTGGCCCT GTGGAAGGAG GTGCTCACTA AGGGGCAGGT GCCCGGTATA 
CGGTGTCTCC AGCAAACAGC AGCCTCCCTG CAGATCCTAG CGGCCCTCTA TCAGCTGCTG 
GCAAAGCCCC TGCAGGCTCT GGAGGTCCTC CTGCTCGTCC AGATCGTCTC ACAGAGACTG 
AAGGACCATG CGAAGGTAGC CGGCTCTTCC TGCCAAGTCA CCCAGCTCCT CCTGACCCTC 
GGCTGTCCCA GCTACGCCCA GGTGCACCTG GAAGAAGCAG AGTCGCGTCT GAAGCTTCTG 
GATCACACCA CAGACACATA TCTGCTCCTC TCCCTGACCT GTGACCTCCT GCGAAGTCGA 
CTCTGCTGCG CTCGCCAGAA GGTGGCTGAG GGTGCTGCTC TGCTGCTGTC TGTGCTTCAG 
GACCCTGCCC TCCAGAGGGC ATCCAAGGCC TGGTACTTGC TGCGCGTCCA GGCCCTGCAG 
CTGGTGGCGG CCTACCTGAG CCTCTCGCCA GACAGCCTGC TGGCCCCGCT GTGGGAGCAA 
CTGTGTGCCC AAGGCTGGCA GACACCTGAG ATAGCACTCA TCGACTCCCA TAAGCTTCTC 
CGAAGTATCA TCCTCCTGCT CATGGGCAGC GATGTGCTCT CAATTCAGAA AACAGCTGTG 
GAGACGCCAT TTCTGGACTA TGGTGAAAAT CTGGTACAAA AATGGCAGGT TCTTTCTGAG 
GTGCTGAGCT GCTCGGAGAA GCTGGTCTCC CGCCTGGGCC GCCTTGGTAA TGTGAGCGAA 
GCGAAAGCCT TTTGCTTGGA GGCCCTGAAA CTTACAACAA AGCTGCAGAT ACCACGCCAG 
TGTGCCCTCT TCCTGGTGCT GAAGGGGGAG CTGGAGCTGG CACGCAATGA CATGGACCTC 
TGTCAGTCGG ACCTGCATCA GGTTCTGTTC TTGCTCGAGT CTTGCACAGA GTTCCTCGGA 
CTAGCGCAGC ACCCGGACTC TGTGAAGAAG GTCCACCTGC AGAAGGGGAA GCAGCGGGCT 
CGGGTGCCCC ATCTTCCAGA TCTCCCGGAG GAAGAGCTCA TTCTAAAAGG CCCTGCCCTG 
GAGCTGGTGG CCACCGTGGC CAAGGAACCT GGCCCTGTAG CACCCTCTGC TAACTCCTCC 
CCGATCCTGA AAACCAAGCC CCAGCCCAGC CCCAGCTTCC TGACCCACTT GCCCACCTGT 
GACTGCTCGC TCTGTGCCAG CCCTGTCCTC TCGGTGGTCT GTCTGCGCTG GGCATTGGTC 
ACAGCGGGGC TGAGACTGGC CATGGGCCAT GAGGCCCAAG GGCTGGATCT GCTGCAGGTG 
GTGCTGAAGG GTTGCCCTGC AGCCGGTAGT CGCCTTGCCC AAGCGCTTCA GGCTTCCCTG 
AATCACACAG CACCCCCCTC CCCTGTCCCA GGCCTCTTTG ATGAGATCTT GGCTCAGGCA 
TATACACAGC TGGCCCTGGA GGGGCTGAGC CAGCCATCAA ACAAGAGCCT GGGAAAGGTC 
TTGGAGTCAG GGTTAACGTT TGTGGTGGCG CGGATACCCC ACCTGGAGCC CTGGCGAGCC 
AACCTGCTCT TGGTCCAGGC CCTTGCAAAG CTGACCAGCC TCAGCTGCTG CACTGCCCAA 
CTTTTTGCAA GCTCCTGGGG CTGGCAGCCG CCATCAATAA ATAACCCCAC AGGCTCAGAA 
CCCTCTAAGC CTCGGAACCA AAAACAGTCA GGACAGTCCA GACGAGGATG CCAGCAAGTG 
GGCTCTGCTA CCCTGCCCCT CTCACATACC TCTCTGAAAG GTCTGGAAGG TGGAGGACCA 
CCCTGTACAC CTAAGCCCCC AGGCCGGGTC AGGCAGGCTG GCCCTGGGGT ACCCTTCACC 
GTATTTGAGG AAGTCTTCCT TACAAAGACC AAGCCTGAGG TTCCCAAGGC CCCCAGGGTA 
CAACGGAGGG TCCAGACACG CCTCAAGGTG AACTTCAGTG ACGACAGTGA CTTGGAAGAC 
CCTGTCTCAG TTGAAGCACA GCTTGCAGAG GGATCCAAGA GACAGGGCAC TGCCTCCCGG 
GGCCGGGGCC GGGCCAGAAA GGGCCCGAGT GTGAAGACCA ACTCAGTGGC CACGTCAGGC 
AGTGCCCCCG GCCTCAGTGG CAGAAGCCGG AGGGTCAAGA AGGTGGCATC AGGACGTTGT 
GAGGAGCTGG GCCCTGAGAT GATGAGGACC ATCCCTGAGG AGGAACTGAC TGACAACCAG 
ATGGAAATGA GCTTTGAGAT CCTCAGGGGT TCTGATGGGG AAGATTTAGC CTCAGGTGGG 
AAGACACCAG CTCCAGCGCC TGATACAGCC GTAGGAGAAT GTGAGGTGTT GAGACGGGAT 
GCCAGCAAAG AGGAGCTGCC CGGCCCAGAC AAGGAGAGAG ACATGGATCT GGGTCCCCGG 
CGCAGACTCC CTTCGGCCCC CATAGCCATT GATCTGTCTA CCCTGGATTC CATCTGTGAC 
TCACTGAGTG GTGCCTTCCG TGGCATCAGT CACTGCCCTC CTAGTGGGCT CTATGCCCAC 
CTCTGCCGCC TCCTGGCCCT GTGCCTGGGC CACCGGGATC CCTATGCCAC CGCCTGCCTT 
ATCACCGAGT CTGTCTCCAT CACCTGTCGC CACCAGCTGC TCACCCACCT CCACAGGCAG 
CTCAACAAGG CCCAGAAGCG CCGAGGGCCG CCGGACATAG CAGACCAGTT GCAGGGGCTG 
CACCTCCAGG AGAAGCCTGG AGACATCCCT CTGGCACGAA TCCAGCGCCT CTTTTCCTTC 
AGGCCTCTGG GATCTGGCCA CTTCCCCCAG CCTGAGAAGG ACAGCTTCCG GGAGCGTCTT 
GCTCTGATTC CCAGTGGGGT GACCGTGTGT GTTTTGGCCC TGGCCACCCT CCAGCCTGGA 
ACTGTGGGCA ACACCCTCCT GCTGACTCGG CTGGAGAAAG ACAAACCCCC GGTCACTGTA 
CAGATCCCCA CTTCCCAGAG CAAGCTTTCT GTGAGTTCGG CCTTGAAAGA GTTTGACGCC 
ATCCAGAAGG AGCAGAAAGA GAACAGCAGC TGTACTGACA AGCGAGAATG GTGGACGGGC 
CGGCTAGAGC TGGATCGCAG GATGGAGGTT CTCACCACTT CCCTAGAGAA GTACGTGCTG 
GGCTGCTGGC GGGGGCTGTT GCTGCCATCC AGTGAGGACC CTGGCCTTGT CCAGGAGGCC 
TCCCGTCTAC AAGAGTCACT ACAGCAATGT GGCTGGAAAT ATCCCGACCC CACTCTGCTG 
AGAATCATGC TCAGCGGTGC CAGTACCCTC ACCCCGCAGG ACGTGCAGGC CCTGGCTTAT 
GGGCTGTGCC CAGCCCGGCC AGCCCGAGCC CAAGAGCTCC TGGGTGAGGC AGTAGGGCGT 
CTCCAAGGTC AGATGGCAAC AAGCAGTAGG CATCTCGTGT TAGTGCTGGA CAAGGACCTA 
CAGAAGCTGC CGTGGGAGAG CATGCCCAGC CTCCGGGCCC TGCCTGTCAC TCGGCTGCCC 
TCCTTCCGAT TCCTACTCAG CTACTCCGTC ATCAAAGAGT CCGGGGCCTC ATCAGTGCTG 
AGCCAAGGAG TGGATCCTCG AAGTACCTTC TACGTCCTGA ACCCTCACAA TAACCTGTCA 
AGCACAGAGG AGCAATTCCG AGCCCATTTT AGCAGTGAAG CTGGCTGGAA AGGGGTGGTT 
GGGGAGGTGC CAAGCCCGGA ACAGGTGCAA ATGGCCCTGA CGGAGCGTGA CTTGTACATC 
TATGCAGGGC ATGGCGCCGG TGCCCGCTTC CTGGATGGGC AGGCTGTCCT GCGGCTGAGC 
TGCCGGGCAG TGACCCTGCT GTTCGGCTGC AGCAGCGCAG CCCTGGCTGT GCATGGAAAC 
CTGGAGGGGG CTGGCATCGT GCTCAAGTAT ATCATGGCTG GCTGCCCCCT GTTTCTAGGT 
AACCTCTGGG ACGTGACTGA CCGTGACATT GACCGCTACA CAGAGGCTCT GTTGCAGGGC 
TGGCTTAGAG CAGGTTCAGG AGCCCCCCTT CTGTACTATG TCAGCCAGGC CCGCCAGGCT 
CCCCGACTCA AGTACCTTAT TGGGGCTGCA CCTGTAGCCT ATGGCTTGCC TGTCTCCCTG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_012174193.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012029583.1 Ovis aries extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.

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CloneID	OSm26796
Clone ID Related Accession (Same CDS sequence)	XM_012174193.2
Accession Version	XM_012174193.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	separin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639012.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012174193.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCT CTGTTGTGCC CTCCGGCACC ATGAGGAGCT TCAGAGGAGT CAACTTTGGG 
ACCCTGCTAA GCAGTCCAAA GGAGGCTGAA GAGCTGCTGC CTGACTTGAA GGAGTTCCTG 
ACCAAGCTTC CCAGCTGCCG GTCTGATTCC GAGAGGAGAC AAACTTGTGA TGCCATTCTG 
AGGGCTTGCA ACCAGCAGCT GACTGTCAAG CTGGCTTGCC CTAGCCACCT GGGAAGCCTT 
CTGGAGCTGG CGGAGCTGGC TTGCGATGGC TACTTGATGT CTACACCACA GCGCCCACCC 
CTCTACCTGG AACGAATTCT TTTCATCTTT CTACGGAACG TTGCTGCACA GGGAGACCCA 
GAAGCCACTC TCCGACTTGC TCAGCCCCTC CATGCCTGCT TGGTGCAATG TCCTCGCCAA 
GCTGCTCCTC AGGATTATGA GGCTGTGGCT CGTGGCAGCT TTTCTCTGCT TTGGAAGGGG 
GCAGAAGGCT TGATGGAGCG GCGAGCTGCA TTCTCAGCTC GGTTAAAGGC CTTGAGCTTT 
CTAGTACTCT TGGAGGATGA AAGTATCCCT TGTGAGATTC CCCACTTTGC TTCTCCAACA 
GCCTGTCGAG TGGTAGCTGC CCATCAGCTG TTTGAAGCCA GTGGGCATGG TCTAAATGAA 
GCAGATGCTG ATTTCCTAGA TGACCTGCTC TCCAGGCATG TGATCACAGC TTTGGTGGGT 
GAAGGAGCCT CTCCTGGTCC TCTTTCCCCC CAGAGGGCCC TCTGTCTCTT GGAGCTCACC 
CTGGAACACT GTCGTCGCCT CTGCTGGAAC CACCACCACA CCAGGGCCAC AAGTACAGTG 
GAGAAGGCCC ATGATTACCT AAGGAACACC AGTCTGGCCC CCAGCCTCCA GCTATGCCAG 
CTGGCAGTTG AACAGTTGCT GGCTGGGGAA GGAGGACCCC AGGCAGTGGC CAAGCTTCTG 
ATCAAGGCAT CAGCTATCCT GAAGAACAGC TTGGAGGCAC CATCACCCCC ACTGCGAGCA 
TTGTGTGACA GCTGCCAGTT CTTCCTCTCA GTCCTGGAGC GAGGCACCAA GAGGTGCTAT 
GGACTTGATG CCATTCTGAG CCTCTTTGCT TTTCTTGGCA GCTACTGTTC CCTCATCCAG 
AAGCTGCAGG ATGGTGTCTG TGGGGACTCC TCCAAGCAGC TGCAGGCTTT GGTTCAGATA 
CACTTTCAGG GACTTCACCT CTACACTGCG GTGGTTTATG ACTTTGCCCA AAGCTGTCAG 
GAAGCTAATT TGGCTGACCT GGCCCAGCTA GCGGACAGTT GCAGATCTAC TGGTGTCTGG 
ATGCTGGAGG CCTTACAGGG CCTACCTGGC CAAGAGCTGA CTGACTACCT GGGGATGACT 
GTCTCGTACA CCAGTAACCT GGCCTACAGC TTCTACAGTC ACAAGCTCTA TGCCGAAGCC 
TGTGCCATCT CCGAGCCCCT CTGTCAGCAC CTGGGCTCAG TGAAGCCAGG CACCTATCCT 
GAGGTGCCAC CTGAGAAGTT GCACAGGTGC TTCCGGCTAC ACATAGAGAG TTTAAAGAAA 
CTGGGTAAAC AGGCCCAGGG CTGCAAGATG GTGACTTTGT GGCTGGAGGC CCTGCAGCCC 
TGTGGCCCCG AACACATGAC TGAGCCAGTC ACCTTCTGGG TTCGGGTCAA GATGGATGCT 
GCCAGAGCTG GAGACAAGGA GCTACAGCTG AAGACGCTGC GAGATAGCCT GAGTGGCTGG 
GACCCGGAGA CCCTGGCCCG CTTGCTCAGG GAGGAGCTGC AGGCCTACAA GGCAGTGCGG 
GCCAACACAG GGCAGGAACG GTTCAATGTC ATCTGTGACC TGCTGGAGCT GAGCCCCGAG 
GAGACAGCAG CCGGGGCCTG GGCCCGAGCC ACCCACCTGG TGGAACTGGC TCAGGTGCTC 
TGCTATCACA ACTTTGCCCA GCAGACTGAC TGCACTGCCC TCGATGCCAT CCGGGAAGCT 
CTGCAGCTTC TGGAATCTGT GAAGCCTGAG GCCCAGGACA AGGATCGGCT TTTGGATGAT 
AAAGCCCAGG CCCTGCTGTG GCTCTACATA TGCACCATGG AGGCCAAAAT GCAGGAGGGT 
ATCGAGCGGG ATCGGAGAGC CCAGGCCCCT TGTAACCTGG AGGAGTTTGA AGCCAATGAC 
CTGAATTATG AAGATAAACT CCAGGAAGAT CGCTTCCTAT ACAGTAACAT TGCCTTCAAC 
CTGGCTGCAG ATGCTGCACA ATCCAAATGC CTGGACCAAG CCCTGGCCCT GTGGAAGGAG 
GTGCTCACTA AGGGGCAGGT GCCCGGTATA CGGTGTCTCC AGCAAACAGC AGCCTCCCTG 
CAGATCCTAG CGGCCCTCTA TCAGCTGCTG GCAAAGCCCC TGCAGGCTCT GGAGGTCCTC 
CTGCTCGTCC AGATCGTCTC ACAGAGACTG AAGGACCATG CGAAGGTAGC CGGCTCTTCC 
TGCCAAGTCA CCCAGCTCCT CCTGACCCTC GGCTGTCCCA GCTACGCCCA GGTGCACCTG 
GAAGAAGCAG AGTCGCGTCT GAAGCTTCTG GATCACACCA CAGACACATA TCTGCTCCTC 
TCCCTGACCT GTGACCTCCT GCGAAGTCGA CTCTGCTGCG CTCGCCAGAA GGTGGCTGAG 
GGTGCTGCTC TGCTGCTGTC TGTGCTTCAG GACCCTGCCC TCCAGAGGGC ATCCAAGGCC 
TGGTACTTGC TGCGCGTCCA GGCCCTGCAG CTGGTGGCGG CCTACCTGAG CCTCTCGCCA 
GACAGCCTGC TGGCCCCGCT GTGGGAGCAA CTGTGTGCCC AAGGCTGGCA GACACCTGAG 
ATAGCACTCA TCGACTCCCA TAAGCTTCTC CGAAGTATCA TCCTCCTGCT CATGGGCAGC 
GATGTGCTCT CAATTCAGAA AACAGCTGTG GAGACGCCAT TTCTGGACTA TGGTGAAAAT 
CTGGTACAAA AATGGCAGGT TCTTTCTGAG GTGCTGAGCT GCTCGGAGAA GCTGGTCTCC 
CGCCTGGGCC GCCTTGGTAA TGTGAGCGAA GCGAAAGCCT TTTGCTTGGA GGCCCTGAAA 
CTTACAACAA AGCTGCAGAT ACCACGCCAG TGTGCCCTCT TCCTGGTGCT GAAGGGGGAG 
CTGGAGCTGG CACGCAATGA CATGGACCTC TGTCAGTCGG ACCTGCATCA GGTTCTGTTC 
TTGCTCGAGT CTTGCACAGA GTTCCTCGGA CTAGCGCAGC ACCCGGACTC TGTGAAGAAG 
GTCCACCTGC AGAAGGGGAA GCAGCGGGCT CGGGTGCCCC ATCTTCCAGA TCTCCCGGAG 
GAAGAGCTCA TTCTAAAAGG CCCTGCCCTG GAGCTGGTGG CCACCGTGGC CAAGGAACCT 
GGCCCTGTAG CACCCTCTGC TAATTCCTCC CCGATCCTGA AAACCAAGCC CCAGCCCAGC 
CCCAGCTTCC TGACCCACTT GCCCACCTGT GACTGCTCGC TCTGTGCCAG CCCTGTCCTC 
TCGGTGGTCT GTCTGCGCTG GGCATTGGTC ACAGCGGGGC TGAGACTGGC CATGGGCCAT 
GAGGCCCAAG GGCTGGATCT GCTGCAGGTG GTGCTGAAGG GTTGCCCTGC AGCCGGTAGT 
CGCCTTGCCC AAGCGCTTCA GGCTTCCCTG AATCACACAG CACCCCCCTC CCCTGTCCCA 
GGCCTCTTTG ATGAGATCTT GGCTCAGGCA TATACACAGC TGGCCCTGGA GGGGCTGAGC 
CAGCCATCAA ACAAGAGCCT GGGAAAGGTC TTGGAGTCAG GGTTAACGTT TGTGGTGGCG 
CGGATACCCC ACCTGGAGCC CTGGCGAGCC AACCTGCTCT TGGTCCAGGC CCTTGCAAAG 
CTGACCAGCC TCAGCTGCTG CACTGCCCAA CTTTTTGCAA GCTCCTGGGG CTGGCAGCCG 
CCATCAATAA ATAACCCCAC AGGCTCAGAA CCCTCTAAGC CTCGGAACCA AAAACAGTCA 
GGACAGTCCA GACGAGGATG CCAGCAAGTG GGCTCTGCTA CCCTGCCCCT CTCACATACC 
TCTCTGAAAG GTCTGGAAGG TGGAGGACCA CCCTGTACAC CTAAGCCCCC AGGCCGGGTC 
AGGCAGGCTG GCCCTGGGGT ACCCTTCACT GTATTTGAGG AAGTCTTCCT TACAAAGACC 
AAGCCTGAGG TTCCCAAGGC CCCCAGGGTA CAACGGAGGG TCCAGACACG CCTCAAGGTG 
AACTTCAGTG ACGACAGTGA CTTGGAAGAC CCTGTCTCAG TTGAAGCACA GCTTGCAGAG 
GGATCCAAGA GACAGGGCAC TGCCTCCCGG GGCCGGGGCC GGGCCAGAAA GGGCCCGAGT 
GTGAAGACCA ACTCAGTGGC CACGTCAGGC AGTGCCCCCG GCCTCAGTGG CAGAAGCCGG 
AGGGTCAAGA AGGTGGCATC AGGACGTTGT GAGGAGCTGG GCCCTGAGAT GATGAGGACC 
ATCCCTGAGG AGGAACTGAC TGACAACCAG ATGGAAATGA GCTTTGAGAT CCTCAGGGGT 
TCTGATGGGG AAGACTTAGC CTCAGGTGGG AAGACACCAG CTCCAGCGCC TGATACAGCC 
GTAGGAGAAT GTGAGGTGTT GAGACGGGAT GCCAGCAAAG AGGAGCTGCC CGGCCCAGAC 
AAGGAGAGAG ACATGGATCT GGGTCCCCGG CGCAGACTCC CTTCGGCCCC CATAGCCATT 
GATCTGTCTA CCCTGGATTC CATCTGTGAC TCACTGAGTG GTGCCTTCCG TGGCATCAGT 
CACTGCCCTC CTAGTGGGCT CTATGCCCAC CTCTGCCGCC TCCTGGCCCT GTGCCTGGGC 
CACCGGGATC CCTATGCCAC CGCCTGCCTT ATCACCGAGT CTGTCTCCAT CACCTGTCGC 
CACCAGCTGC TCACCCACCT CCACAGGCAG CTCAACAAGG CCCAGAAGCG CCGAGGGCCG 
CCGGACATAG CAGACCAGTT GCAGGGGCTG CACCTCCAGG AGAAGCCTGG AGACATCCCT 
CTGGCACGAA TCCAGCACCT CTTTTCCTTC AGGCCTCTGG GATCTGGCCA CTTCCCCCAG 
CCTGAGAAGG ACAGCTTCCG GGAGCGTCTT GCTCTGATTC CCAGTGGGGT GACCGTGTGT 
GTTTTGGCCC TGGCCACCCT CCAGCCTGGA ACTGTGGGCA ACACCCTCCT GCTGACTCGG 
CTGGAGAAAG ACAAACCCCC GGTCACTGTA CAGATCCCCA CTTCCCAGAG CAAGCTTTCT 
GTGAGTTCGG CCTTGAAAGA GTTTGACGCC ATCCAGAAGG AGCAGAAAGA GAACAGCAGC 
TGTACTGACA AGCGAGAATG GTGGACGGGC CGGCTAGAGC TGGATCGCAG GATGGAGGTT 
CTCACCACTT CCCTAGAGAA GTACGTGCTG GGCTGCTGGC GGGGGCTGTT GCTGCCATCC 
AGTGAGGACC CTGGCCTTGT CCAGGAGGCC TCCCGTCTAC AAGAGTCACT ACAGCAATGT 
GGCTGGAAAT ATCCCGACCC CACTCTGCTG AGAATCATGC TCAGCGGTGC CAGTACCCTC 
ACCCCGCAGG ACGTGCAGGC CCTGGCTTAT GGGCTGTGCC CAGCCCGGCC AGCCCGAGCC 
CAAGAGCTCC TGGGTGAGGC AGTAGGGCGT CTCCAAGGTC AGATGGCAAC AAGCAGTAGG 
CATCTCGTGT TAGTGCTGGA CAAGGACCTA CAGAAGCTGC CGTGGGAGAG CATGCCCAGC 
CTCCGGGCCC TGCCTGTCAC TCGGCTGCCC TCCTTCCGAT TCCTACTCAG CTACTCCGTC 
ATCAAAGAGT CCGGGGCCTC ATCAGTGCTG AGCCAAGGAG TGGATCCTCG AAGTACCTTC 
TACGTCCTGA ACCCTCACAA TAACCTGTCA AGCACAGAGG AGCAATTCCG AGCCCATTTT 
AGCAGTGAAG CTGGCTGGAA AGGGGTGGTT GGGGAGGTGC CAAGCCCGGA ACAGGTGCAA 
ACGGCCCTGA CGGAGCGTGA CTTGTACATC TATGCAGGGC ATGGCGCCGG TGCCCGCTTC 
CTGGATGGGC AGGCTGTCCT GCGGCTGAGC TGCCGGGCAG TGACCCTGCT GTTCGGCTGC 
AGCAGCGCAG CCCTGGCTGT GCATGGAAAC CTGGAGGGGG CTGGCATTGT GCTCAAGTAT 
ATCATGGCTG GCTGCCCCCT GTTTCTAGGT AACCTCTGGG ACGTGACTGA CCGTGACATT 
GACCGCTACA CAGAGGCTCT GTTGCAGGGC TGGCTTAGAG CAGGTTCAGG AGCCCCCCTT 
CTGTACTATG TCAGCCAGGC CCGCCAGGCT CCGCGACTCA AGTACCTTAT TGGGGCTGCA 
CCTGTAGCCT ATGGCTTGCC TGTCTCCCTG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012029583.1
CDS	229..6564
Translation	MEASVVPSGTMRSFRGVNFGTLLSSPKEAEELLPDLKEFLTKLPSCRSDSERRQTCDAILRACNQQLTVKLACPSHLGSLLELAELACDGYLMSTPQRPPLYLERILFIFLRNVAAQGDPEATLRLAQPLHACLVQCPRQAAPQDYEAVARGSFSLLWKGAEGLMERRAAFSARLKALSFLVLLEDESIPCEIPHFASPTACRVVAAHQLFEASGHGLNEADADFLDDLLSRHVITALVGEGASPGPLSPQRALCLLELTLEHCRRLCWNHHHTRATSTVEKAHDYLRNTSLAPSLQLCQLAVEQLLAGEGGPQAVAKLLIKASAILKNSLEAPSPPLRALCDSCQFFLSVLERGTKRCYGLDAILSLFAFLGSYCSLIQKLQDGVCGDSSKQLQALVQIHFQGLHLYTAVVYDFAQSCQEANLADLAQLADSCRSTGVWMLEALQGLPGQELTDYLGMTVSYTSNLAYSFYSHKLYAEACAISEPLCQHLGSVKPGTYPEVPPEKLHRCFRLHIESLKKLGKQAQGCKMVTLWLEALQPCGPEHMTEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLARLLREELQAYKAVRANTGQERFNVICDLLELSPEETAAGAWARATHLVELAQVLCYHNFAQQTDCTALDAIREALQLLESVKPEAQDKDRLLDDKAQALLWLYICTMEAKMQEGIERDRRAQAPCNLEEFEANDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKEVLTKGQVPGIRCLQQTAASLQILAALYQLLAKPLQALEVLLLVQIVSQRLKDHAKVAGSSCQVTQLLLTLGCPSYAQVHLEEAESRLKLLDHTTDTYLLLSLTCDLLRSRLCCARQKVAEGAALLLSVLQDPALQRASKAWYLLRVQALQLVAAYLSLSPDSLLAPLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDVLSIQKTAVETPFLDYGENLVQKWQVLSEVLSCSEKLVSRLGRLGNVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDMDLCQSDLHQVLFLLESCTEFLGLAQHPDSVKKVHLQKGKQRARVPHLPDLPEEELILKGPALELVATVAKEPGPVAPSANSSPILKTKPQPSPSFLTHLPTCDCSLCASPVLSVVCLRWALVTAGLRLAMGHEAQGLDLLQVVLKGCPAAGSRLAQALQASLNHTAPPSPVPGLFDEILAQAYTQLALEGLSQPSNKSLGKVLESGLTFVVARIPHLEPWRANLLLVQALAKLTSLSCCTAQLFASSWGWQPPSINNPTGSEPSKPRNQKQSGQSRRGCQQVGSATLPLSHTSLKGLEGGGPPCTPKPPGRVRQAGPGVPFTVFEEVFLTKTKPEVPKAPRVQRRVQTRLKVNFSDDSDLEDPVSVEAQLAEGSKRQGTASRGRGRARKGPSVKTNSVATSGSAPGLSGRSRRVKKVASGRCEELGPEMMRTIPEEELTDNQMEMSFEILRGSDGEDLASGGKTPAPAPDTAVGECEVLRRDASKEELPGPDKERDMDLGPRRRLPSAPIAIDLSTLDSICDSLSGAFRGISHCPPSGLYAHLCRLLALCLGHRDPYATACLITESVSITCRHQLLTHLHRQLNKAQKRRGPPDIADQLQGLHLQEKPGDIPLARIQHLFSFRPLGSGHFPQPEKDSFRERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDKPPVTVQIPTSQSKLSVSSALKEFDAIQKEQKENSSCTDKREWWTGRLELDRRMEVLTTSLEKYVLGCWRGLLLPSSEDPGLVQEASRLQESLQQCGWKYPDPTLLRIMLSGASTLTPQDVQALAYGLCPARPARAQELLGEAVGRLQGQMATSSRHLVLVLDKDLQKLPWESMPSLRALPVTRLPSFRFLLSYSVIKESGASSVLSQGVDPRSTFYVLNPHNNLSSTEEQFRAHFSSEAGWKGVVGEVPSPEQVQTALTERDLYIYAGHGAGARFLDGQAVLRLSCRAVTLLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLRAGSGAPLLYYVSQARQAPRLKYLIGAAPVAYGLPVSLQ

Target ORF information:

RefSeq Version	XM_012174193.2
Organism	Ovis aries(sheep)
Definition	Ovis aries extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012174193.2

ORF Insert Sequence:

ATGGAGGCCT CTGTTGTGCC CTCCGGCACC ATGAGGAGCT TCAGAGGAGT CAACTTTGGG 
ACCCTGCTAA GCAGTCCAAA GGAGGCTGAA GAGCTGCTGC CTGACTTGAA GGAGTTCCTG 
ACCAAGCTTC CCAGCTGCCG GTCTGATTCC GAGAGGAGAC AAACTTGTGA TGCCATTCTG 
AGGGCTTGCA ACCAGCAGCT GACTGTCAAG CTGGCTTGCC CTAGCCACCT GGGAAGCCTT 
CTGGAGCTGG CGGAGCTGGC TTGCGATGGC TACTTGATGT CTACACCACA GCGCCCACCC 
CTCTACCTGG AACGAATTCT TTTCATCTTT CTACGGAACG TTGCTGCACA GGGAGACCCA 
GAAGCCACTC TCCGACTTGC TCAGCCCCTC CATGCCTGCT TGGTGCAATG TCCTCGCCAA 
GCTGCTCCTC AGGATTATGA GGCTGTGGCT CGTGGCAGCT TTTCTCTGCT TTGGAAGGGG 
GCAGAAGGCT TGATGGAGCG GCGAGCTGCA TTCTCAGCTC GGTTAAAGGC CTTGAGCTTT 
CTAGTACTCT TGGAGGATGA AAGTATCCCT TGTGAGATTC CCCACTTTGC TTCTCCAACA 
GCCTGTCGAG TGGTAGCTGC CCATCAGCTG TTTGAAGCCA GTGGGCATGG TCTAAATGAA 
GCAGATGCTG ATTTCCTAGA TGACCTGCTC TCCAGGCATG TGATCACAGC TTTGGTGGGT 
GAAGGAGCCT CTCCTGGTCC TCTTTCCCCC CAGAGGGCCC TCTGTCTCTT GGAGCTCACC 
CTGGAACACT GTCGTCGCCT CTGCTGGAAC CACCACCACA CCAGGGCCAC AAGTACAGTG 
GAGAAGGCCC ATGATTACCT AAGGAACACC AGTCTGGCCC CCAGCCTCCA GCTATGCCAG 
CTGGCAGTTG AACAGTTGCT GGCTGGGGAA GGAGGACCCC AGGCAGTGGC CAAGCTTCTG 
ATCAAGGCAT CAGCTATCCT GAAGAACAGC TTGGAGGCAC CATCACCCCC ACTGCGAGCA 
TTGTGTGACA GCTGCCAGTT CTTCCTCTCA GTCCTGGAGC GAGGCACCAA GAGGTGCTAT 
GGACTTGATG CCATTCTGAG CCTCTTTGCT TTTCTTGGCA GCTACTGTTC CCTCATCCAG 
AAGCTGCAGG ATGGTGTCTG TGGGGACTCC TCCAAGCAGC TGCAGGCTTT GGTTCAGATA 
CACTTTCAGG GACTTCACCT CTACACTGCG GTGGTTTATG ACTTTGCCCA AAGCTGTCAG 
GAAGCTAATT TGGCTGACCT GGCCCAGCTA GCGGACAGTT GCAGATCTAC TGGTGTCTGG 
ATGCTGGAGG CCTTACAGGG CCTACCTGGC CAAGAGCTGA CTGACTACCT GGGGATGACT 
GTCTCGTACA CCAGTAACCT GGCCTACAGC TTCTACAGTC ACAAGCTCTA TGCCGAAGCC 
TGTGCCATCT CCGAGCCCCT CTGTCAGCAC CTGGGCTCAG TGAAGCCAGG CACCTATCCT 
GAGGTGCCAC CTGAGAAGTT GCACAGGTGC TTCCGGCTAC ACATAGAGAG TTTAAAGAAA 
CTGGGTAAAC AGGCCCAGGG CTGCAAGATG GTGACTTTGT GGCTGGAGGC CCTGCAGCCC 
TGTGGCCCCG AACACATGAC TGAGCCAGTC ACCTTCTGGG TTCGGGTCAA GATGGATGCT 
GCCAGAGCTG GAGACAAGGA GCTACAGCTG AAGACGCTGC GAGATAGCCT GAGTGGCTGG 
GACCCGGAGA CCCTGGCCCG CTTGCTCAGG GAGGAGCTGC AGGCCTACAA GGCAGTGCGG 
GCCAACACAG GGCAGGAACG GTTCAATGTC ATCTGTGACC TGCTGGAGCT GAGCCCCGAG 
GAGACAGCAG CCGGGGCCTG GGCCCGAGCC ACCCACCTGG TGGAACTGGC TCAGGTGCTC 
TGCTATCACA ACTTTGCCCA GCAGACTGAC TGCACTGCCC TCGATGCCAT CCGGGAAGCT 
CTGCAGCTTC TGGAATCTGT GAAGCCTGAG GCCCAGGACA AGGATCGGCT TTTGGATGAT 
AAAGCCCAGG CCCTGCTGTG GCTCTACATA TGCACCATGG AGGCCAAAAT GCAGGAGGGT 
ATCGAGCGGG ATCGGAGAGC CCAGGCCCCT TGTAACCTGG AGGAGTTTGA AGCCAATGAC 
CTGAATTATG AAGATAAACT CCAGGAAGAT CGCTTCCTAT ACAGTAACAT TGCCTTCAAC 
CTGGCTGCAG ATGCTGCACA ATCCAAATGC CTGGACCAAG CCCTGGCCCT GTGGAAGGAG 
GTGCTCACTA AGGGGCAGGT GCCCGGTATA CGGTGTCTCC AGCAAACAGC AGCCTCCCTG 
CAGATCCTAG CGGCCCTCTA TCAGCTGCTG GCAAAGCCCC TGCAGGCTCT GGAGGTCCTC 
CTGCTCGTCC AGATCGTCTC ACAGAGACTG AAGGACCATG CGAAGGTAGC CGGCTCTTCC 
TGCCAAGTCA CCCAGCTCCT CCTGACCCTC GGCTGTCCCA GCTACGCCCA GGTGCACCTG 
GAAGAAGCAG AGTCGCGTCT GAAGCTTCTG GATCACACCA CAGACACATA TCTGCTCCTC 
TCCCTGACCT GTGACCTCCT GCGAAGTCGA CTCTGCTGCG CTCGCCAGAA GGTGGCTGAG 
GGTGCTGCTC TGCTGCTGTC TGTGCTTCAG GACCCTGCCC TCCAGAGGGC ATCCAAGGCC 
TGGTACTTGC TGCGCGTCCA GGCCCTGCAG CTGGTGGCGG CCTACCTGAG CCTCTCGCCA 
GACAGCCTGC TGGCCCCGCT GTGGGAGCAA CTGTGTGCCC AAGGCTGGCA GACACCTGAG 
ATAGCACTCA TCGACTCCCA TAAGCTTCTC CGAAGTATCA TCCTCCTGCT CATGGGCAGC 
GATGTGCTCT CAATTCAGAA AACAGCTGTG GAGACGCCAT TTCTGGACTA TGGTGAAAAT 
CTGGTACAAA AATGGCAGGT TCTTTCTGAG GTGCTGAGCT GCTCGGAGAA GCTGGTCTCC 
CGCCTGGGCC GCCTTGGTAA TGTGAGCGAA GCGAAAGCCT TTTGCTTGGA GGCCCTGAAA 
CTTACAACAA AGCTGCAGAT ACCACGCCAG TGTGCCCTCT TCCTGGTGCT GAAGGGGGAG 
CTGGAGCTGG CACGCAATGA CATGGACCTC TGTCAGTCGG ACCTGCATCA GGTTCTGTTC 
TTGCTCGAGT CTTGCACAGA GTTCCTCGGA CTAGCGCAGC ACCCGGACTC TGTGAAGAAG 
GTCCACCTGC AGAAGGGGAA GCAGCGGGCT CGGGTGCCCC ATCTTCCAGA TCTCCCGGAG 
GAAGAGCTCA TTCTAAAAGG CCCTGCCCTG GAGCTGGTGG CCACCGTGGC CAAGGAACCT 
GGCCCTGTAG CACCCTCTGC TAATTCCTCC CCGATCCTGA AAACCAAGCC CCAGCCCAGC 
CCCAGCTTCC TGACCCACTT GCCCACCTGT GACTGCTCGC TCTGTGCCAG CCCTGTCCTC 
TCGGTGGTCT GTCTGCGCTG GGCATTGGTC ACAGCGGGGC TGAGACTGGC CATGGGCCAT 
GAGGCCCAAG GGCTGGATCT GCTGCAGGTG GTGCTGAAGG GTTGCCCTGC AGCCGGTAGT 
CGCCTTGCCC AAGCGCTTCA GGCTTCCCTG AATCACACAG CACCCCCCTC CCCTGTCCCA 
GGCCTCTTTG ATGAGATCTT GGCTCAGGCA TATACACAGC TGGCCCTGGA GGGGCTGAGC 
CAGCCATCAA ACAAGAGCCT GGGAAAGGTC TTGGAGTCAG GGTTAACGTT TGTGGTGGCG 
CGGATACCCC ACCTGGAGCC CTGGCGAGCC AACCTGCTCT TGGTCCAGGC CCTTGCAAAG 
CTGACCAGCC TCAGCTGCTG CACTGCCCAA CTTTTTGCAA GCTCCTGGGG CTGGCAGCCG 
CCATCAATAA ATAACCCCAC AGGCTCAGAA CCCTCTAAGC CTCGGAACCA AAAACAGTCA 
GGACAGTCCA GACGAGGATG CCAGCAAGTG GGCTCTGCTA CCCTGCCCCT CTCACATACC 
TCTCTGAAAG GTCTGGAAGG TGGAGGACCA CCCTGTACAC CTAAGCCCCC AGGCCGGGTC 
AGGCAGGCTG GCCCTGGGGT ACCCTTCACT GTATTTGAGG AAGTCTTCCT TACAAAGACC 
AAGCCTGAGG TTCCCAAGGC CCCCAGGGTA CAACGGAGGG TCCAGACACG CCTCAAGGTG 
AACTTCAGTG ACGACAGTGA CTTGGAAGAC CCTGTCTCAG TTGAAGCACA GCTTGCAGAG 
GGATCCAAGA GACAGGGCAC TGCCTCCCGG GGCCGGGGCC GGGCCAGAAA GGGCCCGAGT 
GTGAAGACCA ACTCAGTGGC CACGTCAGGC AGTGCCCCCG GCCTCAGTGG CAGAAGCCGG 
AGGGTCAAGA AGGTGGCATC AGGACGTTGT GAGGAGCTGG GCCCTGAGAT GATGAGGACC 
ATCCCTGAGG AGGAACTGAC TGACAACCAG ATGGAAATGA GCTTTGAGAT CCTCAGGGGT 
TCTGATGGGG AAGACTTAGC CTCAGGTGGG AAGACACCAG CTCCAGCGCC TGATACAGCC 
GTAGGAGAAT GTGAGGTGTT GAGACGGGAT GCCAGCAAAG AGGAGCTGCC CGGCCCAGAC 
AAGGAGAGAG ACATGGATCT GGGTCCCCGG CGCAGACTCC CTTCGGCCCC CATAGCCATT 
GATCTGTCTA CCCTGGATTC CATCTGTGAC TCACTGAGTG GTGCCTTCCG TGGCATCAGT 
CACTGCCCTC CTAGTGGGCT CTATGCCCAC CTCTGCCGCC TCCTGGCCCT GTGCCTGGGC 
CACCGGGATC CCTATGCCAC CGCCTGCCTT ATCACCGAGT CTGTCTCCAT CACCTGTCGC 
CACCAGCTGC TCACCCACCT CCACAGGCAG CTCAACAAGG CCCAGAAGCG CCGAGGGCCG 
CCGGACATAG CAGACCAGTT GCAGGGGCTG CACCTCCAGG AGAAGCCTGG AGACATCCCT 
CTGGCACGAA TCCAGCACCT CTTTTCCTTC AGGCCTCTGG GATCTGGCCA CTTCCCCCAG 
CCTGAGAAGG ACAGCTTCCG GGAGCGTCTT GCTCTGATTC CCAGTGGGGT GACCGTGTGT 
GTTTTGGCCC TGGCCACCCT CCAGCCTGGA ACTGTGGGCA ACACCCTCCT GCTGACTCGG 
CTGGAGAAAG ACAAACCCCC GGTCACTGTA CAGATCCCCA CTTCCCAGAG CAAGCTTTCT 
GTGAGTTCGG CCTTGAAAGA GTTTGACGCC ATCCAGAAGG AGCAGAAAGA GAACAGCAGC 
TGTACTGACA AGCGAGAATG GTGGACGGGC CGGCTAGAGC TGGATCGCAG GATGGAGGTT 
CTCACCACTT CCCTAGAGAA GTACGTGCTG GGCTGCTGGC GGGGGCTGTT GCTGCCATCC 
AGTGAGGACC CTGGCCTTGT CCAGGAGGCC TCCCGTCTAC AAGAGTCACT ACAGCAATGT 
GGCTGGAAAT ATCCCGACCC CACTCTGCTG AGAATCATGC TCAGCGGTGC CAGTACCCTC 
ACCCCGCAGG ACGTGCAGGC CCTGGCTTAT GGGCTGTGCC CAGCCCGGCC AGCCCGAGCC 
CAAGAGCTCC TGGGTGAGGC AGTAGGGCGT CTCCAAGGTC AGATGGCAAC AAGCAGTAGG 
CATCTCGTGT TAGTGCTGGA CAAGGACCTA CAGAAGCTGC CGTGGGAGAG CATGCCCAGC 
CTCCGGGCCC TGCCTGTCAC TCGGCTGCCC TCCTTCCGAT TCCTACTCAG CTACTCCGTC 
ATCAAAGAGT CCGGGGCCTC ATCAGTGCTG AGCCAAGGAG TGGATCCTCG AAGTACCTTC 
TACGTCCTGA ACCCTCACAA TAACCTGTCA AGCACAGAGG AGCAATTCCG AGCCCATTTT 
AGCAGTGAAG CTGGCTGGAA AGGGGTGGTT GGGGAGGTGC CAAGCCCGGA ACAGGTGCAA 
ACGGCCCTGA CGGAGCGTGA CTTGTACATC TATGCAGGGC ATGGCGCCGG TGCCCGCTTC 
CTGGATGGGC AGGCTGTCCT GCGGCTGAGC TGCCGGGCAG TGACCCTGCT GTTCGGCTGC 
AGCAGCGCAG CCCTGGCTGT GCATGGAAAC CTGGAGGGGG CTGGCATTGT GCTCAAGTAT 
ATCATGGCTG GCTGCCCCCT GTTTCTAGGT AACCTCTGGG ACGTGACTGA CCGTGACATT 
GACCGCTACA CAGAGGCTCT GTTGCAGGGC TGGCTTAGAG CAGGTTCAGG AGCCCCCCTT 
CTGTACTATG TCAGCCAGGC CCGCCAGGCT CCGCGACTCA AGTACCTTAT TGGGGCTGCA 
CCTGTAGCCT ATGGCTTGCC TGTCTCCCTG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_027967256.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027823057.1 Ovis aries extra spindle pole bodies like 1, separase (ESPL1), mRNA.

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CloneID	OSm77755
Clone ID Related Accession (Same CDS sequence)	XM_027967256.1
Accession Version	XM_027967256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	separin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAGAGGAGT CAACTTTGGG ACCCTGCTAA GCAGTCCAAA GGAGGCTGAA 
GAGCTGCTGC CTGACTTGAA GGAGTTCCTG ACCAAGCTTC CCAGCTGCCG GTCTGATTCC 
GAGAGGAGAC AAACTTGTGA TGCCATTCTG AGGGCTTGCA ACCAGCAGCT GACTGTCAAG 
CTGGCTTGCC CTAGGCACCT GGGAAGCCTT CTGGAGCTGG CGGAGCTGGC TTGCGATGGC 
TACTTGATGT CTACACCACA GCGCCCACCC CTCTACCTGG AACGAATTCT TTTCATCTTT 
CTACGGAACG TTGCTGCACA GGGAGACCCA GAAGCCACTC TCCGACTTGC TCAGCCCCTC 
CATGCCTGCT TGGTGCAATG TCCTCGCCAA GCTGCTCCTC AGGACTATGA GGCTGTGGCT 
CGTGGCAGCT TTTCTCTGCT TTGGAAGGGG GCAGAAGGCT TGATGGAGCG GCGAGCTGCA 
TTCTCAGCTC GGTTAAAGGC CTTGAGCTTT CTAGTACTCT TGGAGGATGA AAGTATCCCT 
TGTGAGATTC CCCACTTTGC TTCTCCAACA GCCTGTCGAG TGGTAGCTGC CCATCAGCTG 
TTTGAAGCCA GTGGGCATGG TCTAAATGAA GCAGATGCTG ATTTCCTAGA TGACCTGCTC 
TCCAGGCATG TGATCACAGC TTTGGTGGGT GAAGGAGCCT CTCCTGGTCC TCTTTCCCCC 
CAGAGGGCCC TCTGTCTCTT GGAGCTCACC CTGGAACACT GTCGTCGCCT CTGCTGGAAC 
CACCACCACA CCAGGGCCAC AAGTACAGTG GAGAAGGCCC ATGATTACCT AAGGAACACC 
AGTCTGGCCC CCAGCCTCCA GCTATGCCAG CTGGCAGTTG AACAGTTGCT GGCTGGGGAA 
GGAGGACCCC AGGCAGTGGC CAAGCTTCTG ATCAAGGCAT CAGCTATCCT GAAGAACAGC 
TTGGAGGCAC CATCACCCCC ACTGCGAGCA TTGTGTGACA GCTGCCAGTT CTTCCTCTCA 
GTCCTGGAGC GAGGCACCAA GAGGTGCTAT GGACTTGATG CCATTCTGAG CCTCTTTGCT 
TTTCTTGGCA GCTACTGTTC CCTCATCCAG AAGCTGCAGG ATGGTGTCTG TGGGGACTCC 
TCCAAGCAGC TGCAGGCTTT GGTTCAGATA CACTTTCAGG GACTTCACCT CTACACTGCG 
GTGGTTTATG ACTTTGCCCA AAGCTGTCAG GAAGCTAATT TGGCTGACCT GGCCCAGCTA 
GCGGACAGTT GCAGATCTAC TGGTATCTGG ATGCTGGAGG CCTTACAGGG CCTACCTGGC 
CAAGAGCTGA CTGACTACCT GGGGATGACT GTCTCGTACA CCAGTAACCT GGCCTACAGC 
TTCTACAGTC ACAAGCTCTA TGCCGAAGCC TGTGCCATCT CCGAGCCCCT CTGTCAGCAC 
CTGGGCTCAG TGAAGCCAGG CACCTATCCT GAGGTGCCAC CTGAGAAGTT GCACAGGTGC 
TTCCGGCTAC ACATAGAGAG TTTAAAGAAA CTGGGTAAAC AGGCCCAGGG CTGCAAGATG 
GTGACTTTGT GGCTGGAGGC CCTGCAGCCC TGTGGCCCCG AACACATGAC TGAGCCAGTC 
ACCTTCTGGG TTCGGGTCAA GATGGATGCT GCCAGAGCTG GAGACAAGGA GCTACAGCTG 
AAGACGCTGC GAGATAGCCT GAGTGGCTGG GACCCGGAGA CCCTGGCCCG CTTGCTCAGG 
GAGGAGTTGC AGGCCTACAA GGCAGTGCGG GCCAACACAG GGCAGGAACG GTTCAATGTC 
ATCTGTGACC TGCTGGAGCT GAGCCCCGAG GAGACAGCAG CCGGGGCCTG GGCCCGAGCC 
ACCCACCTGG TGGAACTGGC TCAGGTGCTC TGCTATCACA ACTTTGCCCA GCAGACTGAC 
TGCACTGCCC TCGATGCCAT CCGGGAAGCT CTGCAGCTTC TGGAATCTGT GAAGCCGGAG 
GCCCAGGACA AGGATCGGCT TTTGGATGAT AAAGCCCAGG CCCTGCTGTG GCTCTACATA 
TGCACCATGG AGGCCAAAAT GCAGGAGGGT ATCGAGCGGG ATCGGAGAGC CCAGGCCCCT 
TGTAACCTGG AGGAGTTTGA AGCCAATGAC CTGAATTATG AAGATAAACT CCAGGAAGAT 
CGCTTCCTAT ACAGTAACAT TGCCTTCAAC CTGGCTGCGG ATGCTGCACA ATCCAAATGC 
CTGGACCAAG CCCTGGCCCT GTGGAAGGAG GTGCTTACTA AGGGGCAGGT GCCCGGTATA 
CGGTGTCTCC AGCAAACAGC AGCCTCCCTG CAGATCCTAG CGGCCCTCTA TCAGCTGCTG 
GCAAAGCCCC TGCAGGCTCT GGAGGTCCTC CTGCTCGTCC AGATCGTCTC ACAGAGACTG 
AAGGACCATG CGAAGGTAGC CAGCTCTTCC TGCCAAGTCA CCCAGCTCCT CCTGACCCTC 
GGCTGTCCCA GCTACGCCCA GGTGCACCTG GAAGAAGCAG AGTCGCGTCT GAAGCTTCTG 
GATCACACCA CAGACACATA TCTGCTCCTC TCCCTGACCT GTGACCTCCT GCGAAGTCGA 
CTCTGCTGCG CTCGCCAGAA GGTGGCTGAG GGTGCTGCTC TGCTGCTGTC TGTGCTTCAG 
GACCCTGCCC TCCAGAGGGC ATCCAAGGCC TGGTACTTGC TGCGCGTCCA GGCCCTGCAG 
CTGGTGGCGG CCTACCTGAG CCTCTCGCCA GACAGCCTGC TGGCCCCGCT GTGGGAGCAA 
CTGTGTGCCC AAGGCTGGCA GACACCTGAG ATAGCACTCA TCGACTCCCA TAAGCTTCTC 
CGAAGTATCA TCCTCCTGCT CATGGGCAGC GATGTGCTCT CAATTCAGAA AACAGCTGTG 
GAGACGCCAT TTCTGGACTA TGGTGAAAAT CTGGTACAAA AATGGCAGGT TCTTTCTGAG 
GTGCTGAGCT GCTCGGAGAA GCTGGTCTCC CGCCTGGGCC GCCTTGGTAA TGTGAGCGAA 
GCGAAAGCCT TTTGCTTGGA GGCCCTGAAA CTTACAACAA AGCTGCAGAT ACCACGCCAG 
TGTGCCCTCT TCCTGGTGCT GAAGGGGGAG CTGGAGCTGG CACGCAATGA CATGGACCTC 
TGTCAGTCGG ACCTGCATCA GGTTCTGTTC TTGCTCGAGT CTTGCACAGA GTTCCTCGGA 
CTAGCGCAGC ACCCGGACTC TGTGAAGAAG GTCCACCTGC AGAAGGGGAA GCAGCGGGCT 
CGGGTGCCCC ATCTTCCAGA TCTCCCGGAG GAAGAGCTCA TTCTAAAAGG CCCTGCCCTG 
GAGCTGGTGG CCACCGTGGC CAAGGAACCT GGCCCTGTAG CACCCTCTGC TAATTCCTCC 
CCGATCCTGA AAACCAAGCC CCAGCCCAGC CCCAGCTTCC TGACCCACTT GCCCACCTGT 
GACTGCTCGC TCTGTGCCAG CCCTGTCCTC TCGGTGGTCT GTCTGCGCTG GGCATTGGTC 
ACAGCGGGGC TGAGACTGGC CATGGGCCAT GAGGCCCAAG GGCTGGATCT GCTGCAGGTG 
GTGCTGAAGG GTTGCCCTGC AGCCGGTAGT CGCCTTGCCC AAGCGCTGCA GGCTTCCCTG 
AATCACACAG CACCCCCCTC CCCTGTCCCA GGCCTCTTTG ATGAGATCTT GGCTCAGGCA 
TATACACAGC TGGCCCTGGA GGGGCTGAGC CAGCCATCAA ACAAGAGCCT GGGAAAGGTC 
TTGGAGTCAG GGTTAACGTT TGTGGTGGCG CGGATACCCC ACCTGGAGCC CTGGCGAGCC 
AACCTGCTCT TGGTCCAGGC CCTTGCAAAG CTGACCAGCC TCAGCTGCTG CACTGCCCAA 
CTTTTTGCAA GCTCCTGGGG CTGGCAGCCG CCATCAATAA ATAACCCCAC AGGCTCAGAA 
CCCTCTAAGC CTCGGAACCA AAAACAGTCA GGACAGTCCA GACGAGGATG CCAGCAAGTG 
GGCTCTGCTA CCCTGCCCCT CTCACATACC TCTCTGAAAG GTCTGGAAGG TGGAGGACCA 
CCCTGTACAC CTAAGCCCCC AGGCCGGGTC AGGCAGGCTG GCCCTGGGGT ACCCTTCACC 
GTATTTGAGG AAGTCTTCCT TACAAAGACC AAGCCTGAGG TTCCCAAGGC CCCCAGGGTA 
CAACGGAGGG TCCAGACACG CCTCAAGGTG AACTTCAGTG ACGACAGTGA CTTGGAAGAC 
CCTGTCTCAG TTGAAGCACA GCTTGCAGAG GGATCCAAGA GACAGGGCAG TGCCTCCCGG 
GGCCGGGGCC GGGCCAGAAA GGGCCCGAGT GTGAAGACCA ACTCAGTGGC CACGTCAGGC 
AGTGCCCCCG GCCTCAGTGG CAGAAGCCGG AGGGTCAAGA AGGTGGCATC AGGACGTTGT 
GAGGAGCTGG GCCCTGAGAT GATGAGGACC ATCCCTGAGG AGGAACTGAC TGACAACCAG 
ATGGAAATGA GCTTTGAGAT CCTCAGGGGT TCTGATGGGG AAGACTTAGC CTCAGGTGGG 
AAGACACCAG CTCCAGCGCC TGATACAGCC GTAGGAGAAT GTGAGGTGTT GAGACGGGAT 
GCCAGCAAAG AGGAGCTGCC CGGCCCAGAC AAGGAGAGAG ACATGGATCT GGGTCCCCGG 
CGCAGACTCC CTTCGGCCCC CATAGCCATT GATCTGTCTA CCCTGGATTC CATCTGTGAC 
TCACTGAGTG GTGCCTTCCG TGGCATCAGT CACTGCCCTC CTAGTGGGCT CTATGCCCAC 
CTCTGCCGCC TCCTGGCCCT GTGCCTGGGC CACCGGGATC CCTATGCCAC CGCCTGCCTT 
ATCACCGAGT CTGTCTCCAT CACCTGTCGC CACCAGCTGC TCACCCACCT CCACAGGCAG 
CTCAACAAGG CCCAGAAGCG CCGAGGGCCG CCGGACATAG CAGACCAGTT GCAGGGGCTG 
CACCTCCAGG AGAAGCCTGG AGACATCCCT CTGGCACGAA TCCAGCGCCT CTTTTCCTTC 
AGGCCTCTGG GATCTGGCCA CTTCCCCCAG CCTGAGAAGG ACAGCTTCCG GGAGCGTCTT 
GCTCTGATTC CCAGTGGGGT GACCGTGTGT GTTTTGGCCC TGGCCACCCT CCAGCCTGGA 
ACTGTGGGCA ACACCCTCCT GCTGACTCGG CTGGAGAAAG ACAAACCCCC GGTCACTGTA 
CAGATCCCCA CTTCCCAGAG CAAGCTTTCT GTGAGTTCGG CCTTGAAAGA GTTTGACGCC 
ATCCAGAAGG AGCAGAAAGA GAACAGCAGC TGTACTGACA AGCGAGAATG GTGGACGGGC 
CGGCTAGAGC TGGATCGCAG GATGGAGGTT CTCACCACTT CCCTAGAGAA GTACGTGCTG 
GGCTGCTGGC GGGGGCTGTT GCTGCCATCC AGTGAGGACC CTGGCCTTGT CCAGGAGGCC 
TCCCGTCTAC AAGAGTCACT ACAGCAATGT GGCTGGAAAT ATCCCGACCC CACTCTGCTG 
AGAATCATGC TCAGCGGTGC CAGTACCCTC ACCCCGCAGG ACGTGCAGGC CCTGGCTTAT 
GGGCTGTGCC CAGCCCGGCC AGCCCGAGCC CAAGAGCTCC TGGGTGAGGC AGTAGGGCGT 
CTCCAAGGTC AGATGGCAAC AAGCAGTAGG CATCTCGTGT TAGTGCTGGA CAAGGACCTA 
CAGAAGCTGC CGTGGGAGAG CATGCCCAGC CTCCGGGCCC TGCCTGTCAC TCGGCTGCCC 
TCCTTCCGAT TCCTACTCAG CTACTCCGTC ATCAAAGAGT CCGGGGCCTC ATCAGTGCTG 
AGCCAAGGAG TGGATCCTCG AAGTACCTTC TACGTCCTGA ACCCTCACAA TAACCTGTCA 
AGCACAGAGG AGCAATTCCG AGCCCATTTT AGCAGTGAAG CTGGCTGGAA AGGGGTGGTT 
GGGGAGGTGC CAAGCCCGGA ACAGGTGCAA ACGGCCCTGA CGGAGCGTGA CTTGTACATC 
TATGCAGGGC ATGGCGCCGG TGCCCGCTTC CTGGATGGGC AGGCTGTCCT GCGGCTGAGC 
TGCCGGGCAG TGACCCTGCT GTTCGGCTGC AGCAGCGCAG CCCTGGCTGT GCATGGAAAC 
CTGGAGGGGG CTGGCATCGT GCTCAAGTAT ATCATGGCTG GCTGCCCCCT GTTTCTAGGT 
AACCTCTGGG ACGTGACTGA CCGTGACATT GACCGCTACA CAGAGGCTCT GTTGCAGGGA 
TGGCTTAGAG CAGGTTCAGG AGCCCCCCTT CTGTACTATG TCAGCCAGGC CCGCCAGGCT 
CCCCGACTCA AGTACCTTAT TGGGGCTGCA CCTGTAGCCT ATGGCTTGCC TGTCTCCCTG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027823057.1
CDS	58..6363
Translation	MRSFRGVNFGTLLSSPKEAEELLPDLKEFLTKLPSCRSDSERRQTCDAILRACNQQLTVKLACPRHLGSLLELAELACDGYLMSTPQRPPLYLERILFIFLRNVAAQGDPEATLRLAQPLHACLVQCPRQAAPQDYEAVARGSFSLLWKGAEGLMERRAAFSARLKALSFLVLLEDESIPCEIPHFASPTACRVVAAHQLFEASGHGLNEADADFLDDLLSRHVITALVGEGASPGPLSPQRALCLLELTLEHCRRLCWNHHHTRATSTVEKAHDYLRNTSLAPSLQLCQLAVEQLLAGEGGPQAVAKLLIKASAILKNSLEAPSPPLRALCDSCQFFLSVLERGTKRCYGLDAILSLFAFLGSYCSLIQKLQDGVCGDSSKQLQALVQIHFQGLHLYTAVVYDFAQSCQEANLADLAQLADSCRSTGIWMLEALQGLPGQELTDYLGMTVSYTSNLAYSFYSHKLYAEACAISEPLCQHLGSVKPGTYPEVPPEKLHRCFRLHIESLKKLGKQAQGCKMVTLWLEALQPCGPEHMTEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLARLLREELQAYKAVRANTGQERFNVICDLLELSPEETAAGAWARATHLVELAQVLCYHNFAQQTDCTALDAIREALQLLESVKPEAQDKDRLLDDKAQALLWLYICTMEAKMQEGIERDRRAQAPCNLEEFEANDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKEVLTKGQVPGIRCLQQTAASLQILAALYQLLAKPLQALEVLLLVQIVSQRLKDHAKVASSSCQVTQLLLTLGCPSYAQVHLEEAESRLKLLDHTTDTYLLLSLTCDLLRSRLCCARQKVAEGAALLLSVLQDPALQRASKAWYLLRVQALQLVAAYLSLSPDSLLAPLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDVLSIQKTAVETPFLDYGENLVQKWQVLSEVLSCSEKLVSRLGRLGNVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDMDLCQSDLHQVLFLLESCTEFLGLAQHPDSVKKVHLQKGKQRARVPHLPDLPEEELILKGPALELVATVAKEPGPVAPSANSSPILKTKPQPSPSFLTHLPTCDCSLCASPVLSVVCLRWALVTAGLRLAMGHEAQGLDLLQVVLKGCPAAGSRLAQALQASLNHTAPPSPVPGLFDEILAQAYTQLALEGLSQPSNKSLGKVLESGLTFVVARIPHLEPWRANLLLVQALAKLTSLSCCTAQLFASSWGWQPPSINNPTGSEPSKPRNQKQSGQSRRGCQQVGSATLPLSHTSLKGLEGGGPPCTPKPPGRVRQAGPGVPFTVFEEVFLTKTKPEVPKAPRVQRRVQTRLKVNFSDDSDLEDPVSVEAQLAEGSKRQGSASRGRGRARKGPSVKTNSVATSGSAPGLSGRSRRVKKVASGRCEELGPEMMRTIPEEELTDNQMEMSFEILRGSDGEDLASGGKTPAPAPDTAVGECEVLRRDASKEELPGPDKERDMDLGPRRRLPSAPIAIDLSTLDSICDSLSGAFRGISHCPPSGLYAHLCRLLALCLGHRDPYATACLITESVSITCRHQLLTHLHRQLNKAQKRRGPPDIADQLQGLHLQEKPGDIPLARIQRLFSFRPLGSGHFPQPEKDSFRERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDKPPVTVQIPTSQSKLSVSSALKEFDAIQKEQKENSSCTDKREWWTGRLELDRRMEVLTTSLEKYVLGCWRGLLLPSSEDPGLVQEASRLQESLQQCGWKYPDPTLLRIMLSGASTLTPQDVQALAYGLCPARPARAQELLGEAVGRLQGQMATSSRHLVLVLDKDLQKLPWESMPSLRALPVTRLPSFRFLLSYSVIKESGASSVLSQGVDPRSTFYVLNPHNNLSSTEEQFRAHFSSEAGWKGVVGEVPSPEQVQTALTERDLYIYAGHGAGARFLDGQAVLRLSCRAVTLLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLRAGSGAPLLYYVSQARQAPRLKYLIGAAPVAYGLPVSLQ

Target ORF information:

RefSeq Version	XM_027967256.1
Organism	Ovis aries(sheep)
Definition	Ovis aries extra spindle pole bodies like 1, separase (ESPL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027967256.1

ORF Insert Sequence:

ATGAGGAGCT TCAGAGGAGT CAACTTTGGG ACCCTGCTAA GCAGTCCAAA GGAGGCTGAA 
GAGCTGCTGC CTGACTTGAA GGAGTTCCTG ACCAAGCTTC CCAGCTGCCG GTCTGATTCC 
GAGAGGAGAC AAACTTGTGA TGCCATTCTG AGGGCTTGCA ACCAGCAGCT GACTGTCAAG 
CTGGCTTGCC CTAGGCACCT GGGAAGCCTT CTGGAGCTGG CGGAGCTGGC TTGCGATGGC 
TACTTGATGT CTACACCACA GCGCCCACCC CTCTACCTGG AACGAATTCT TTTCATCTTT 
CTACGGAACG TTGCTGCACA GGGAGACCCA GAAGCCACTC TCCGACTTGC TCAGCCCCTC 
CATGCCTGCT TGGTGCAATG TCCTCGCCAA GCTGCTCCTC AGGACTATGA GGCTGTGGCT 
CGTGGCAGCT TTTCTCTGCT TTGGAAGGGG GCAGAAGGCT TGATGGAGCG GCGAGCTGCA 
TTCTCAGCTC GGTTAAAGGC CTTGAGCTTT CTAGTACTCT TGGAGGATGA AAGTATCCCT 
TGTGAGATTC CCCACTTTGC TTCTCCAACA GCCTGTCGAG TGGTAGCTGC CCATCAGCTG 
TTTGAAGCCA GTGGGCATGG TCTAAATGAA GCAGATGCTG ATTTCCTAGA TGACCTGCTC 
TCCAGGCATG TGATCACAGC TTTGGTGGGT GAAGGAGCCT CTCCTGGTCC TCTTTCCCCC 
CAGAGGGCCC TCTGTCTCTT GGAGCTCACC CTGGAACACT GTCGTCGCCT CTGCTGGAAC 
CACCACCACA CCAGGGCCAC AAGTACAGTG GAGAAGGCCC ATGATTACCT AAGGAACACC 
AGTCTGGCCC CCAGCCTCCA GCTATGCCAG CTGGCAGTTG AACAGTTGCT GGCTGGGGAA 
GGAGGACCCC AGGCAGTGGC CAAGCTTCTG ATCAAGGCAT CAGCTATCCT GAAGAACAGC 
TTGGAGGCAC CATCACCCCC ACTGCGAGCA TTGTGTGACA GCTGCCAGTT CTTCCTCTCA 
GTCCTGGAGC GAGGCACCAA GAGGTGCTAT GGACTTGATG CCATTCTGAG CCTCTTTGCT 
TTTCTTGGCA GCTACTGTTC CCTCATCCAG AAGCTGCAGG ATGGTGTCTG TGGGGACTCC 
TCCAAGCAGC TGCAGGCTTT GGTTCAGATA CACTTTCAGG GACTTCACCT CTACACTGCG 
GTGGTTTATG ACTTTGCCCA AAGCTGTCAG GAAGCTAATT TGGCTGACCT GGCCCAGCTA 
GCGGACAGTT GCAGATCTAC TGGTATCTGG ATGCTGGAGG CCTTACAGGG CCTACCTGGC 
CAAGAGCTGA CTGACTACCT GGGGATGACT GTCTCGTACA CCAGTAACCT GGCCTACAGC 
TTCTACAGTC ACAAGCTCTA TGCCGAAGCC TGTGCCATCT CCGAGCCCCT CTGTCAGCAC 
CTGGGCTCAG TGAAGCCAGG CACCTATCCT GAGGTGCCAC CTGAGAAGTT GCACAGGTGC 
TTCCGGCTAC ACATAGAGAG TTTAAAGAAA CTGGGTAAAC AGGCCCAGGG CTGCAAGATG 
GTGACTTTGT GGCTGGAGGC CCTGCAGCCC TGTGGCCCCG AACACATGAC TGAGCCAGTC 
ACCTTCTGGG TTCGGGTCAA GATGGATGCT GCCAGAGCTG GAGACAAGGA GCTACAGCTG 
AAGACGCTGC GAGATAGCCT GAGTGGCTGG GACCCGGAGA CCCTGGCCCG CTTGCTCAGG 
GAGGAGTTGC AGGCCTACAA GGCAGTGCGG GCCAACACAG GGCAGGAACG GTTCAATGTC 
ATCTGTGACC TGCTGGAGCT GAGCCCCGAG GAGACAGCAG CCGGGGCCTG GGCCCGAGCC 
ACCCACCTGG TGGAACTGGC TCAGGTGCTC TGCTATCACA ACTTTGCCCA GCAGACTGAC 
TGCACTGCCC TCGATGCCAT CCGGGAAGCT CTGCAGCTTC TGGAATCTGT GAAGCCGGAG 
GCCCAGGACA AGGATCGGCT TTTGGATGAT AAAGCCCAGG CCCTGCTGTG GCTCTACATA 
TGCACCATGG AGGCCAAAAT GCAGGAGGGT ATCGAGCGGG ATCGGAGAGC CCAGGCCCCT 
TGTAACCTGG AGGAGTTTGA AGCCAATGAC CTGAATTATG AAGATAAACT CCAGGAAGAT 
CGCTTCCTAT ACAGTAACAT TGCCTTCAAC CTGGCTGCGG ATGCTGCACA ATCCAAATGC 
CTGGACCAAG CCCTGGCCCT GTGGAAGGAG GTGCTTACTA AGGGGCAGGT GCCCGGTATA 
CGGTGTCTCC AGCAAACAGC AGCCTCCCTG CAGATCCTAG CGGCCCTCTA TCAGCTGCTG 
GCAAAGCCCC TGCAGGCTCT GGAGGTCCTC CTGCTCGTCC AGATCGTCTC ACAGAGACTG 
AAGGACCATG CGAAGGTAGC CAGCTCTTCC TGCCAAGTCA CCCAGCTCCT CCTGACCCTC 
GGCTGTCCCA GCTACGCCCA GGTGCACCTG GAAGAAGCAG AGTCGCGTCT GAAGCTTCTG 
GATCACACCA CAGACACATA TCTGCTCCTC TCCCTGACCT GTGACCTCCT GCGAAGTCGA 
CTCTGCTGCG CTCGCCAGAA GGTGGCTGAG GGTGCTGCTC TGCTGCTGTC TGTGCTTCAG 
GACCCTGCCC TCCAGAGGGC ATCCAAGGCC TGGTACTTGC TGCGCGTCCA GGCCCTGCAG 
CTGGTGGCGG CCTACCTGAG CCTCTCGCCA GACAGCCTGC TGGCCCCGCT GTGGGAGCAA 
CTGTGTGCCC AAGGCTGGCA GACACCTGAG ATAGCACTCA TCGACTCCCA TAAGCTTCTC 
CGAAGTATCA TCCTCCTGCT CATGGGCAGC GATGTGCTCT CAATTCAGAA AACAGCTGTG 
GAGACGCCAT TTCTGGACTA TGGTGAAAAT CTGGTACAAA AATGGCAGGT TCTTTCTGAG 
GTGCTGAGCT GCTCGGAGAA GCTGGTCTCC CGCCTGGGCC GCCTTGGTAA TGTGAGCGAA 
GCGAAAGCCT TTTGCTTGGA GGCCCTGAAA CTTACAACAA AGCTGCAGAT ACCACGCCAG 
TGTGCCCTCT TCCTGGTGCT GAAGGGGGAG CTGGAGCTGG CACGCAATGA CATGGACCTC 
TGTCAGTCGG ACCTGCATCA GGTTCTGTTC TTGCTCGAGT CTTGCACAGA GTTCCTCGGA 
CTAGCGCAGC ACCCGGACTC TGTGAAGAAG GTCCACCTGC AGAAGGGGAA GCAGCGGGCT 
CGGGTGCCCC ATCTTCCAGA TCTCCCGGAG GAAGAGCTCA TTCTAAAAGG CCCTGCCCTG 
GAGCTGGTGG CCACCGTGGC CAAGGAACCT GGCCCTGTAG CACCCTCTGC TAATTCCTCC 
CCGATCCTGA AAACCAAGCC CCAGCCCAGC CCCAGCTTCC TGACCCACTT GCCCACCTGT 
GACTGCTCGC TCTGTGCCAG CCCTGTCCTC TCGGTGGTCT GTCTGCGCTG GGCATTGGTC 
ACAGCGGGGC TGAGACTGGC CATGGGCCAT GAGGCCCAAG GGCTGGATCT GCTGCAGGTG 
GTGCTGAAGG GTTGCCCTGC AGCCGGTAGT CGCCTTGCCC AAGCGCTGCA GGCTTCCCTG 
AATCACACAG CACCCCCCTC CCCTGTCCCA GGCCTCTTTG ATGAGATCTT GGCTCAGGCA 
TATACACAGC TGGCCCTGGA GGGGCTGAGC CAGCCATCAA ACAAGAGCCT GGGAAAGGTC 
TTGGAGTCAG GGTTAACGTT TGTGGTGGCG CGGATACCCC ACCTGGAGCC CTGGCGAGCC 
AACCTGCTCT TGGTCCAGGC CCTTGCAAAG CTGACCAGCC TCAGCTGCTG CACTGCCCAA 
CTTTTTGCAA GCTCCTGGGG CTGGCAGCCG CCATCAATAA ATAACCCCAC AGGCTCAGAA 
CCCTCTAAGC CTCGGAACCA AAAACAGTCA GGACAGTCCA GACGAGGATG CCAGCAAGTG 
GGCTCTGCTA CCCTGCCCCT CTCACATACC TCTCTGAAAG GTCTGGAAGG TGGAGGACCA 
CCCTGTACAC CTAAGCCCCC AGGCCGGGTC AGGCAGGCTG GCCCTGGGGT ACCCTTCACC 
GTATTTGAGG AAGTCTTCCT TACAAAGACC AAGCCTGAGG TTCCCAAGGC CCCCAGGGTA 
CAACGGAGGG TCCAGACACG CCTCAAGGTG AACTTCAGTG ACGACAGTGA CTTGGAAGAC 
CCTGTCTCAG TTGAAGCACA GCTTGCAGAG GGATCCAAGA GACAGGGCAG TGCCTCCCGG 
GGCCGGGGCC GGGCCAGAAA GGGCCCGAGT GTGAAGACCA ACTCAGTGGC CACGTCAGGC 
AGTGCCCCCG GCCTCAGTGG CAGAAGCCGG AGGGTCAAGA AGGTGGCATC AGGACGTTGT 
GAGGAGCTGG GCCCTGAGAT GATGAGGACC ATCCCTGAGG AGGAACTGAC TGACAACCAG 
ATGGAAATGA GCTTTGAGAT CCTCAGGGGT TCTGATGGGG AAGACTTAGC CTCAGGTGGG 
AAGACACCAG CTCCAGCGCC TGATACAGCC GTAGGAGAAT GTGAGGTGTT GAGACGGGAT 
GCCAGCAAAG AGGAGCTGCC CGGCCCAGAC AAGGAGAGAG ACATGGATCT GGGTCCCCGG 
CGCAGACTCC CTTCGGCCCC CATAGCCATT GATCTGTCTA CCCTGGATTC CATCTGTGAC 
TCACTGAGTG GTGCCTTCCG TGGCATCAGT CACTGCCCTC CTAGTGGGCT CTATGCCCAC 
CTCTGCCGCC TCCTGGCCCT GTGCCTGGGC CACCGGGATC CCTATGCCAC CGCCTGCCTT 
ATCACCGAGT CTGTCTCCAT CACCTGTCGC CACCAGCTGC TCACCCACCT CCACAGGCAG 
CTCAACAAGG CCCAGAAGCG CCGAGGGCCG CCGGACATAG CAGACCAGTT GCAGGGGCTG 
CACCTCCAGG AGAAGCCTGG AGACATCCCT CTGGCACGAA TCCAGCGCCT CTTTTCCTTC 
AGGCCTCTGG GATCTGGCCA CTTCCCCCAG CCTGAGAAGG ACAGCTTCCG GGAGCGTCTT 
GCTCTGATTC CCAGTGGGGT GACCGTGTGT GTTTTGGCCC TGGCCACCCT CCAGCCTGGA 
ACTGTGGGCA ACACCCTCCT GCTGACTCGG CTGGAGAAAG ACAAACCCCC GGTCACTGTA 
CAGATCCCCA CTTCCCAGAG CAAGCTTTCT GTGAGTTCGG CCTTGAAAGA GTTTGACGCC 
ATCCAGAAGG AGCAGAAAGA GAACAGCAGC TGTACTGACA AGCGAGAATG GTGGACGGGC 
CGGCTAGAGC TGGATCGCAG GATGGAGGTT CTCACCACTT CCCTAGAGAA GTACGTGCTG 
GGCTGCTGGC GGGGGCTGTT GCTGCCATCC AGTGAGGACC CTGGCCTTGT CCAGGAGGCC 
TCCCGTCTAC AAGAGTCACT ACAGCAATGT GGCTGGAAAT ATCCCGACCC CACTCTGCTG 
AGAATCATGC TCAGCGGTGC CAGTACCCTC ACCCCGCAGG ACGTGCAGGC CCTGGCTTAT 
GGGCTGTGCC CAGCCCGGCC AGCCCGAGCC CAAGAGCTCC TGGGTGAGGC AGTAGGGCGT 
CTCCAAGGTC AGATGGCAAC AAGCAGTAGG CATCTCGTGT TAGTGCTGGA CAAGGACCTA 
CAGAAGCTGC CGTGGGAGAG CATGCCCAGC CTCCGGGCCC TGCCTGTCAC TCGGCTGCCC 
TCCTTCCGAT TCCTACTCAG CTACTCCGTC ATCAAAGAGT CCGGGGCCTC ATCAGTGCTG 
AGCCAAGGAG TGGATCCTCG AAGTACCTTC TACGTCCTGA ACCCTCACAA TAACCTGTCA 
AGCACAGAGG AGCAATTCCG AGCCCATTTT AGCAGTGAAG CTGGCTGGAA AGGGGTGGTT 
GGGGAGGTGC CAAGCCCGGA ACAGGTGCAA ACGGCCCTGA CGGAGCGTGA CTTGTACATC 
TATGCAGGGC ATGGCGCCGG TGCCCGCTTC CTGGATGGGC AGGCTGTCCT GCGGCTGAGC 
TGCCGGGCAG TGACCCTGCT GTTCGGCTGC AGCAGCGCAG CCCTGGCTGT GCATGGAAAC 
CTGGAGGGGG CTGGCATCGT GCTCAAGTAT ATCATGGCTG GCTGCCCCCT GTTTCTAGGT 
AACCTCTGGG ACGTGACTGA CCGTGACATT GACCGCTACA CAGAGGCTCT GTTGCAGGGA 
TGGCTTAGAG CAGGTTCAGG AGCCCCCCTT CTGTACTATG TCAGCCAGGC CCGCCAGGCT 
CCCCGACTCA AGTACCTTAT TGGGGCTGCA CCTGTAGCCT ATGGCTTGCC TGTCTCCCTG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ESPL1 cDNA ORF clone, Ovis aries(sheep)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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