TCOF1 cDNA ORF clone, Physeter catodon(sperm whale)

Gene
Clones
gRNAs

Summary

Gene Symbol	TCOF1
Entrez Gene ID	102994443
Full Name	treacle ribosome biogenesis factor 1
Gene Type	protein-coding
Organism	Physeter catodon(sperm whale)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007109872.1	XP_007109934.1	treacle protein isoform X2
XM_007109871.1	XP_007109933.1	treacle protein isoform X1
XM_024124753.1	XP_023980521.1	treacle protein
XM_028493453.1	XP_028349254.1	treacle protein

Pathways from BioSystems
Gene Ontology

The following TCOF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TCOF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPh331333	XM_028493453.1 Latest version!	Physeter catodon treacle ribosome biogenesis factor 1 (TCOF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OPh118878	XM_007109871.1 Latest version!	Physeter catodon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPh118879	XM_007109872.1 Latest version!	Physeter catodon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPh284670	XM_024124753.1 Latest version!	Physeter catodon treacle ribosome biogenesis factor 1 (TCOF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TCOF1 ( XM_028493453.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_028349254.1 Physeter catodon treacle ribosome biogenesis factor 1 (TCOF1), mRNA.

Price & Availability↑

CloneID	OPh331333
Clone ID Related Accession (Same CDS sequence)	XM_028493453.1
Accession Version	XM_028493453.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	treacle protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041221.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGGTCTCTA TCAGGCCTGA CGGCAACATC TTAGATCATG ACATTGTGAG AGTGGGGCCC 
AAGGAGGACC CTTACTGTGC TTGGGGGATT GATTGCTGCT TTCTCTTTAC TTCTCTGCAG 
AAAAGTTTCC TGACTCAGCC TGTAACCCTT CTGGACATCT ATACACACTG GCAACAAACC 
TCGGAGATTG GCCAGAAGCG GAAGGCAGAG GAAGATGTAG CCCTGCAGGC CAAGAAGACC 
CGCGTGTCAG ACCCCATTAG CAGCTCAGAG AGCTCAGAAG AGGAGGAGGA GGAGGAGCAG 
GGGGCAGAAG CGGAAACCAC CAAAGCCATC CCGGCACTAG CATCTACCAA CTCCTCAGCC 
ACGGGGACGG TTTTGCCTTC AAGTGTGAAA GAAAAAGCCA AGGCAAAGAC CAAGAAAGCC 
AGCAAGACGG TGAACTCCAC GGCACACCCT GCCTCCGGGA AGGCAGTGGC CCACCTCCTC 
ACCAAGAAGT CACCCCAGAA GGCGGCAGGG CCCTCGGCAA ATACCATCCT GGTCTCAGAA 
ACTGAGGAGG AGGGCGGTGT CTCGGCCCTC AGAACCACTG CCAAGCCTGG AATGGCGGCC 
GCCAGCCAGG CCGCCAGCTC CAGCGAGGAC ACCTCCAGCT CGAGCGATGA GACGGACGTG 
GAGGTGAAAC CTGCAGTAAA ACCACCACAG GTCAAAGCCT CAGCAGCTGC GGCCAAGGAG 
TCTCCAAGGA GAAGGGCAGC CCCGACCCCA GGGAAGGCGG GACATGTGAC ACCCCAAGTC 
AGAGGGGGTG CCATGACCCC AGCCAGCAGG GCCAAGAAGC CAGAAGAGAT CCCAGAGAGC 
AGCGAGGAGT CCGAGAGTGA GGAGGGGGCC CCTGCGGGGA CACCCAGCCA GGTAAAGGCC 
TCTGAGAAAA CTGTCCAGGT CAGAGCTGCC TCGGGTCCTG CCAAGGGGAC CCCTGGGAAA 
GGGGCCACCC CAGCACCCCC TGGGAAGGCA GGGAGGCCAG AGGAGGACTC GGAGAGCAGC 
AGCGAGGAGT CGGACAGCGA GGAGGAGACG CCAGCTGCCC AGACCCTGCT GCAGGCAAAG 
GCCTCAGGGA AGCTCCCCCA GGTCAGAGCC GTCGCAGCAG CACCTGCCCA GGTGCTTTCC 
CCTGGGAAAG GGGCCCCTCC AGCACCCCCT GGCAAGGCAG GGCCTGCAGC CATCCAGGCC 
CAGACCAAGG GGCAGGAGGA GGACTCAGAG AGCAGCAGCG AGGAGTCAGA CAGCGAGGGG 
GAGGCGTCCA CAGCTGTGCC TCACACCACA AGCCTGGCTC AGGCAAAGCC CTTGGGGAAA 
AACTCCCAGG TCAGAGCTGC CTCAGCCGCA AGCACGGGGC CCTCAGCGAA GAGCGCTGTC 
CCAGCGCTCC CTCGGAAAGC AAGGGCTGTA GCCGCCCAGG TGGCAAAGTG GGATGAGGAC 
TCGGAGAGCA GCAGCGAGGA GGAGTCGGAC AGCGAGGGGG AGGCGCTGAG GGCAGCGGCC 
TTGGCCCAGG CAAAGTCCTC GGGGAAAGTC CTGCAGGTCA GACCTGCCTC AGGTCCCACC 
CAGGGCCCCC CTCAGAAGGC CGGGCCTGCA GCCGCCCAGG TCAAGACCAA AAGGTCCAAG 
GAAGACTCGG AGAGCAGTGA GGAGGAGTCA GACAGTGAGG ACGAGGCCCC CACAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTCAGACCA AGATCTCCCC AAGGAAGGGC 
ACCCCCATTA CACCCGCATC TGCCAAGGTC CCACCAGTGC AAGTAAGCGC ACCAGCCCCC 
TGGAAAGCAG GAACGGTGAC TTCGCCAACC TGCACATCAT CCCCAGCCGT GGTCAGGGGC 
ACCCAGAGGC CAGAGGAAGA CTCTTCAAGC AGCGAGGAGC CGGAGAGTGA GGAGGAGACA 
GCTCCTGCCG CATCCGTGGG ACAGGTGAAG TCTGTAGGGA AAAGCCTCCC TGTGAAAGCT 
GCCTCGGCAC CCACCAAGGG GCCCTCAGGG CAACGGACCA CCTCAGGGCT CCCTGGGAAG 
GCAGGGCCTG CAGTGGCCCA GGTCAAAGCT GAGGTGCAGG AAGACTCAGA GAGCAGTGAG 
GAGGAGTCGG ACAATGAGGA GGCGGCCTCA GCTGCAGCAC AGGTGAAGAC CTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CCCAGCTGCC ACCAGAGTGG CTTCAGCCAA AGGGGCACCA 
TCAGCTCCTG GAAAAGGGGT TTCTGCAGCT GCTCAAGCCA AACTGGGGTC CCAAGCCAAG 
GTAAAGCCAC CAGCAAGAGC CCCCCAGAGC AGTGCCGTCT CGGGCAGGGG CCAAGTGTCT 
GTGCCGGCTA TGGGGAAGGC ACTGGCTGCA GCAGCCCAGG CCCAGCTGGG GCCGGTCAGG 
GGCCCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG GGAGGCGCCC 
ACTCAGGCGA AGCCCTCAGG GAAGACCCCC CAGGTCAGAA CTGCCTCAGC CTCCACCAAG 
GGGTCCCCCA GGAAAGGGGC TGGCCCTGCA CCCCCTGGGA AGGCAGGAGC CCCAGCCGCC 
CAGGCCGGGA AGCGGAAAGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC AGACAATGAC 
GGGGAGACGC CGGCCGCTGT GCCCCCAGCC CAGGTGAGGC CTCATGAGGA AGCAGCTACA 
GTGCCAGGCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028349254.1
CDS	1..2715
Translation	MVSIRPDGNILDHDIVRVGPKEDPYCAWGIDCCFLFTSLQKSFLTQPVTLLDIYTHWQQTSEIGQKRKAEEDVALQAKKTRVSDPISSSESSEEEEEEEQGAEAETTKAIPALASTNSSATGTVLPSSVKEKAKAKTKKASKTVNSTAHPASGKAVAHLLTKKSPQKAAGPSANTILVSETEEEGGVSALRTTAKPGMAAASQAASSSEDTSSSSDETDVEVKPAVKPPQVKASAAAAKESPRRRAAPTPGKAGHVTPQVRGGAMTPASRAKKPEEIPESSEESESEEGAPAGTPSQVKASEKTVQVRAASGPAKGTPGKGATPAPPGKAGRPEEDSESSSEESDSEEETPAAQTLLQAKASGKLPQVRAVAAAPAQVLSPGKGAPPAPPGKAGPAAIQAQTKGQEEDSESSSEESDSEGEASTAVPHTTSLAQAKPLGKNSQVRAASAASTGPSAKSAVPALPRKARAVAAQVAKWDEDSESSSEEESDSEGEALRAAALAQAKSSGKVLQVRPASGPTQGPPQKAGPAAAQVKTKRSKEDSESSEEESDSEDEAPTAKTPAQAKSAVKTQTKISPRKGTPITPASAKVPPVQVSAPAPWKAGTVTSPTCTSSPAVVRGTQRPEEDSSSSEEPESEEETAPAASVGQVKSVGKSLPVKAASAPTKGPSGQRTTSGLPGKAGPAVAQVKAEVQEDSESSEEESDNEEAASAAAQVKTSVKTPQTKANPAATRVASAKGAPSAPGKGVSAAAQAKLGSQAKVKPPARAPQSSAVSGRGQVSVPAMGKALAAAAQAQLGPVRGPQEDSESSEEESDSEGEAPTQAKPSGKTPQVRTASASTKGSPRKGAGPAPPGKAGAPAAQAGKRKEDSESSSEEESDNDGETPAAVPPAQVRPHEEAATVPGL

Target ORF information:

RefSeq Version	XM_028493453.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon treacle ribosome biogenesis factor 1 (TCOF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028493453.1

ORF Insert Sequence:

ATGGTCTCTA TCAGGCCTGA CGGCAACATC TTAGATCATG ACATTGTGAG AGTGGGGCCC 
AAGGAGGACC CTTACTGTGC TTGGGGGATT GATTGCTGCT TTCTCTTTAC TTCTCTGCAG 
AAAAGTTTCC TGACTCAGCC TGTAACCCTT CTGGACATCT ATACACACTG GCAACAAACC 
TCGGAGATTG GCCAGAAGCG GAAGGCAGAG GAAGATGTAG CCCTGCAGGC CAAGAAGACC 
CGCGTGTCAG ACCCCATTAG CAGCTCAGAG AGCTCAGAAG AGGAGGAGGA GGAGGAGCAG 
GGGGCAGAAG CGGAAACCAC CAAAGCCATC CCGGCACTAG CATCTACCAA CTCCTCAGCC 
ACGGGGACGG TTTTGCCTTC AAGTGTGAAA GAAAAAGCCA AGGCAAAGAC CAAGAAAGCC 
AGCAAGACGG TGAACTCCAC GGCACACCCT GCCTCCGGGA AGGCAGTGGC CCACCTCCTC 
ACCAAGAAGT CACCCCAGAA GGCGGCAGGG CCCTCGGCAA ATACCATCCT GGTCTCAGAA 
ACTGAGGAGG AGGGCGGTGT CTCGGCCCTC AGAACCACTG CCAAGCCTGG AATGGCGGCC 
GCCAGCCAGG CCGCCAGCTC CAGCGAGGAC ACCTCCAGCT CGAGCGATGA GACGGACGTG 
GAGGTGAAAC CTGCAGTAAA ACCACCACAG GTCAAAGCCT CAGCAGCTGC GGCCAAGGAG 
TCTCCAAGGA GAAGGGCAGC CCCGACCCCA GGGAAGGCGG GACATGTGAC ACCCCAAGTC 
AGAGGGGGTG CCATGACCCC AGCCAGCAGG GCCAAGAAGC CAGAAGAGAT CCCAGAGAGC 
AGCGAGGAGT CCGAGAGTGA GGAGGGGGCC CCTGCGGGGA CACCCAGCCA GGTAAAGGCC 
TCTGAGAAAA CTGTCCAGGT CAGAGCTGCC TCGGGTCCTG CCAAGGGGAC CCCTGGGAAA 
GGGGCCACCC CAGCACCCCC TGGGAAGGCA GGGAGGCCAG AGGAGGACTC GGAGAGCAGC 
AGCGAGGAGT CGGACAGCGA GGAGGAGACG CCAGCTGCCC AGACCCTGCT GCAGGCAAAG 
GCCTCAGGGA AGCTCCCCCA GGTCAGAGCC GTCGCAGCAG CACCTGCCCA GGTGCTTTCC 
CCTGGGAAAG GGGCCCCTCC AGCACCCCCT GGCAAGGCAG GGCCTGCAGC CATCCAGGCC 
CAGACCAAGG GGCAGGAGGA GGACTCAGAG AGCAGCAGCG AGGAGTCAGA CAGCGAGGGG 
GAGGCGTCCA CAGCTGTGCC TCACACCACA AGCCTGGCTC AGGCAAAGCC CTTGGGGAAA 
AACTCCCAGG TCAGAGCTGC CTCAGCCGCA AGCACGGGGC CCTCAGCGAA GAGCGCTGTC 
CCAGCGCTCC CTCGGAAAGC AAGGGCTGTA GCCGCCCAGG TGGCAAAGTG GGATGAGGAC 
TCGGAGAGCA GCAGCGAGGA GGAGTCGGAC AGCGAGGGGG AGGCGCTGAG GGCAGCGGCC 
TTGGCCCAGG CAAAGTCCTC GGGGAAAGTC CTGCAGGTCA GACCTGCCTC AGGTCCCACC 
CAGGGCCCCC CTCAGAAGGC CGGGCCTGCA GCCGCCCAGG TCAAGACCAA AAGGTCCAAG 
GAAGACTCGG AGAGCAGTGA GGAGGAGTCA GACAGTGAGG ACGAGGCCCC CACAGCCAAG 
ACTCCAGCCC AGGCAAAATC AGCTGTGAAA ACTCAGACCA AGATCTCCCC AAGGAAGGGC 
ACCCCCATTA CACCCGCATC TGCCAAGGTC CCACCAGTGC AAGTAAGCGC ACCAGCCCCC 
TGGAAAGCAG GAACGGTGAC TTCGCCAACC TGCACATCAT CCCCAGCCGT GGTCAGGGGC 
ACCCAGAGGC CAGAGGAAGA CTCTTCAAGC AGCGAGGAGC CGGAGAGTGA GGAGGAGACA 
GCTCCTGCCG CATCCGTGGG ACAGGTGAAG TCTGTAGGGA AAAGCCTCCC TGTGAAAGCT 
GCCTCGGCAC CCACCAAGGG GCCCTCAGGG CAACGGACCA CCTCAGGGCT CCCTGGGAAG 
GCAGGGCCTG CAGTGGCCCA GGTCAAAGCT GAGGTGCAGG AAGACTCAGA GAGCAGTGAG 
GAGGAGTCGG ACAATGAGGA GGCGGCCTCA GCTGCAGCAC AGGTGAAGAC CTCAGTGAAA 
ACCCCTCAGA CCAAAGCCAA CCCAGCTGCC ACCAGAGTGG CTTCAGCCAA AGGGGCACCA 
TCAGCTCCTG GAAAAGGGGT TTCTGCAGCT GCTCAAGCCA AACTGGGGTC CCAAGCCAAG 
GTAAAGCCAC CAGCAAGAGC CCCCCAGAGC AGTGCCGTCT CGGGCAGGGG CCAAGTGTCT 
GTGCCGGCTA TGGGGAAGGC ACTGGCTGCA GCAGCCCAGG CCCAGCTGGG GCCGGTCAGG 
GGCCCACAGG AGGACTCGGA GAGCAGTGAG GAGGAGTCGG ACAGTGAGGG GGAGGCGCCC 
ACTCAGGCGA AGCCCTCAGG GAAGACCCCC CAGGTCAGAA CTGCCTCAGC CTCCACCAAG 
GGGTCCCCCA GGAAAGGGGC TGGCCCTGCA CCCCCTGGGA AGGCAGGAGC CCCAGCCGCC 
CAGGCCGGGA AGCGGAAAGA GGACTCGGAG AGCAGCAGCG AGGAGGAGTC AGACAATGAC 
GGGGAGACGC CGGCCGCTGT GCCCCCAGCC CAGGTGAGGC CTCATGAGGA AGCAGCTACA 
GTGCCAGGCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_007109871.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007109933.1 Physeter catodon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPh118878
Clone ID Related Accession (Same CDS sequence)	XM_007109871.1
Accession Version	XM_007109871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	treacle protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006713815.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGCGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AAGTTTCCTG 
ACTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC GGAGATTGGC 
CAGAAGCGGA AGGCAGAGGA AGATGTAGCC CTGCAGGCCA AGAAGACCCG CGTGTCAGAC 
CCCATTAGCA GCTCAGAGAG CTCAGAAGAG GAGGAGGAGG AGGAGCAGGG GGCAGAAGCG 
GAAACCACCA AAGCCATCCC GGCACTAGCA TCTACCAACT CCTCAGCCAC GGGGACGGTT 
TTGCCTTCAA GTGTGAAAGA AAAAGCCAAG GCAAAGACCA AGAAAGCCAG CAAGACGGTG 
AACTCCACGG CACACCCTGC CTCCGGGAAG GCAGTGGCCC ACCTCCTCAC CAAGAAGTCA 
CCCCAGAAGG CGGCAGGGCC CTCGGCAAAT ACCATCCTGG TCTCAGAAAC TGAGGAGGAG 
GGCGGTGTCT CGGCCCTCAG AACCACTGCC AAGCCTGGAA TGGCGGCCGC CAGCCAGGCC 
GCCAGCTCCA GCGAGGACAC CTCCAGCTCG AGCGATGAGA CGGACGTGGA GGTGAAACCT 
GCAGTAAAAC CACCACAGGT CAAAGCCTCA GCAGCTGCGG CCAAGGAGTC TCCAAGGAGA 
AGGGCAGCCC CGACCCCAGG GAAGGCGGGA CATGTGACAC CCCAAGTCAG AGGGGGTGCC 
ATGACCCCAG CCAGCAGGGC CAAGAAGCCA GAAGAGATCC CAGAGAGCAG CGAGGAGTCC 
GAGAGTGAGG AGGGGGCCCC TGCGGGGACA CCCAGCCAGG TAAAGGCCTC TGAGAAAACT 
GTCCAGGTCA GAGCTGCCTC GGGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GAGGCCAGAG GAGGACTCGG AGAGCAGCAG CGAGGAGTCG 
GACAGCGAGG AGGAGACGCC AGCTGCCCAG ACCCTGCTGC AGGCAAAGGC CTCAGGGAAG 
CTCCCCCAGG TCAGAGCCGT CGCAGCAGCA CCTGCCCAGG TGCTTTCCCC TGGGAAAGGG 
GCCCCTCCAG CACCCCCTGG CAAGGCAGGG CCTGCAGCCA TCCAGGCCCA GACCAAGGGG 
CAGGAGGAGG ACTCAGAGAG CAGCAGCGAG GAGTCAGACA GCGAGGGGGA GGCGTCCACA 
GCTGTGCCTC ACACCACAAG CCTGGCTCAG GCAAAGCCCT TGGGGAAAAA CTCCCAGGTC 
AGAGCTGCCT CAGCCGCAAG CACGGGGCCC TCAGCGAAGA GCGCTGTCCC AGCGCTCCCT 
CGGAAAGCAA GGGCTGTAGC CGCCCAGGTG GCAAAGTGGG ATGAGGACTC GGAGAGCAGC 
AGCGAGGAGG AGTCGGACAG CGAGGGGGAG GCGCTGAGGG CAGCGGCCTT GGCCCAGGCA 
AAGTCCTCGG GGAAAGTCCT GCAGGTCAGA CCTGCCTCAG GTCCCACCCA GGGCCCCCCT 
CAGAAGGCCG GGCCTGCAGC CGCCCAGGTC AAGACCAAAA GGTCCAAGGA AGACTCGGAG 
AGCAGTGAGG AGGAGTCAGA CAGTGAGGAC GAGGCCCCCA CAGCCAAGAC TCCAGCCCAG 
GCAAAATCAG CTGTGAAAAC TCAGACCAAG ATCTCCCCAA GGAAGGGCAC CCCCATTACA 
CCCGCATCTG CCAAGGTCCC ACCAGTGCAA GTAAGCGCAC CAGCCCCCTG GAAAGCAGGA 
ACGGTGACTT CGCCAACCTG CACATCATCC CCAGCCGTGG TCAGGGGCAC CCAGAGGCCA 
GAGGAAGACT CTTCAAGCAG CGAGGAGCCG GAGAGTGAGG AGGAGACAGC TCCTGCCGCA 
TCCGTGGGAC AGGTGAAGTC TGTAGGGAAA AGCCTCCCTG TGAAAGCTGC CTCGGCACCC 
ACCAAGGGGC CCTCAGGGCA ACGGACCACC TCAGGGCTCC CTGGGAAGGC AGGGCCTGCA 
GTGGCCCAGG TCAAAGCTGA GGTGCAGGAA GACTCAGAGA GCAGTGAGGA GGAGTCGGAC 
AATGAGGAGG CGGCCTCAGC TGCAGCACAG GTGAAGACCT CAGTGAAAAC CCCTCAGACC 
AAAGCCAACC CAGCTGCCAC CAGAGTGGCT TCAGCCAAAG GGGCACCATC AGCTCCTGGA 
AAAGGGGTTT CTGCAGCTGC TCAAGCCAAA CTGGGGTCCC AAGCCAAGGT AAAGCCACCA 
GCAAGAGCCC CCCAGAGCAG TGCCGTCTCG GGCAGGGGCC AAGTGTCTGT GCCGGCTATG 
GGGAAGGCAC TGGCTGCAGC AGCCCAGGCC CAGCTGGGGC CGGTCAGGGG CCCACAGGAG 
GACTCGGAGA GCAGTGAGGA GGAGTCGGAC AGTGAGGGGG AGGCGCCCAC TCAGGCGAAG 
CCCTCAGGGA AGACCCCCCA GGTCAGAACT GCCTCAGCCT CCACCAAGGG GTCCCCCAGG 
AAAGGGGCTG GCCCTGCACC CCCTGGGAAG GCAGGAGCCC CAGCCGCCCA GGCCGGGAAG 
CGGAAAGAGG ACTCGGAGAG CAGCAGCGAG GAGGAGTCAG ACAATGACGG GGAGACGCCG 
GCCGCTGTGC CCCCAGCCCA GAAGGAGGGT AACTCCAAAA CTGCCAGAAG CAAGACCCTG 
GCCCCAGCGC CCACGGAGAA GAAGGCAGAG GGGTCCTCAA CCAGCAGTGA TGAGCTGCCA 
GCGAGACAGG TGATTAAAAC CCCTCTGATT TTTGTCGACC CTAATCGTAG TCCAGCTGGC 
CCAGCTGCTA CCCCCGCCCA AGCCCAGGCT ACAAGCACCC CGAGGAAGGC CCAGGCCTCA 
GAGAGCACAC CCAGGAGCTC CTCTGAGAGT GAGGATGAGG ACGTGATCCC CGCTACGCAG 
TGCTCGACTC CTGCCATTAG GACCAGCGTG GCGACTGTGC CCACGGCCCA CCCCAGGGCA 
GCCCTCAGAG CCAGCATGGT GGGGGCCGGC AGCAGTGAGG ACACCAGTCG GGTGTCTGAA 
GGCAAGAAAC AGGAGACGCC AGCCACTCAG GTGACAAAGA GGAACCCAGC TCACCTCCCG 
CTGACCCAGG CTGCCCTGAA GGTCCTTGCC CAGAAAGCCA GTGAGGCCCA GCCTCCTGCT 
GCCAGGACCC TGTCTTCAAG TGGGACCGAC AGTGCTCTGG GAACACTGCC CATGACGAGT 
CCCCAGAGCA CCCCCGCACA GGCCAGAGGG GCCAACAAGC TCAGAAAGCC TGAGGTTCCT 
GCAACCCAGC GGGCCATAGC CACTCCCTCG GGACGCCCCA AGGCCAAGGC CTCTGGGACC 
TCAGATGACA GCAAGGACAG CAGTGGCAGC TCTTCAGGGA GTGAAGAAGA TGCTGAGGGG 
CCCCAGGTGG CCCCACTGAC CCACAGGCCA GGCCCAGCCC CCTCCAGGAG GGAGACTTTG 
GTGGAAGAGA CCACAGCAGA CTCCAGCGAT GATGAGATGG TGGAACCTTC CCAGTCTCTC 
CTCTCAGGTT ATGTGACCCC TGGGCTGACT CCGGCCAGTT CCCAGGCTTC AAAGGCCACT 
CCCAAGCCAG ACCCCAACCC CTCGGTTTCC TCTACTCCGG CCACCAGAGA TGCCCCGGAC 
GGCAAGCAGG AAGTGGAGCC CCAACAAGCA GCAGGCACCA TGTCCCCTAA AACAGGCAGA 
AGAGAGGCTA GCGCCACACC TCAGAAGCCC CAGAAGACCG GGAAGGGGCC CAGGAGCCCC 
CAGGCCTCCG TGCTGGCGCT GCAGAGCGAC ATCGCCCGGC GCCTGCTGAG CGAGCCCTGG 
CCCCTGAGTG AGGCGCAGGT GCAGGCCTCG GTGGTGAAGG TGTTGGCGGA GCTGCTGGAG 
CAGGAGAAGA AGAAGGCCGT GGATGCCACC AAGGAGGGCA GCCGGAAGGG CCGCCTGGGC 
AACAAGCGGA AGCTCTCCGA AGACCAGACG GCCGCCGGTG CCCCCAGGAG CAAGAAGAAG 
AAGCGGCTGG CAGCCGGGGA TGGCGGGGAG CGTGCAGTCT CCCCAGAAAA GGCCCCCAGG 
ACTTCCAAGG GGAAATCCAA GAGGGACAAA GCGAGTGGTG ACATCAAGGA GAAGAAGGAG 
AAGGAGTCTC CCGGCTCCCA AGGGGCCAAG GAGAAGCCAG AAGGGGAGCC GGGGAAGGTG 
AAGGGTGAGG GGGGAGACCA AGGCAACCCA AAGAGCAAGA AGGAGAAGAA GAAATCCGAC 
AAGAAAAAAA AAGACAAGGA AAAAAAAGAA AAGAAGAAGA AAGCAAAAAA GGCCTCAACC 
AAAGACCCTG ACTCACCATT GCAGAAGAAA AAGAAGAAAA AGAAGAAGAC GGCAGAGCAA 
ACTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007109933.1
CDS	1..4389
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKSFLTQPVTLLDIYTHWQQTSEIGQKRKAEEDVALQAKKTRVSDPISSSESSEEEEEEEQGAEAETTKAIPALASTNSSATGTVLPSSVKEKAKAKTKKASKTVNSTAHPASGKAVAHLLTKKSPQKAAGPSANTILVSETEEEGGVSALRTTAKPGMAAASQAASSSEDTSSSSDETDVEVKPAVKPPQVKASAAAAKESPRRRAAPTPGKAGHVTPQVRGGAMTPASRAKKPEEIPESSEESESEEGAPAGTPSQVKASEKTVQVRAASGPAKGTPGKGATPAPPGKAGRPEEDSESSSEESDSEEETPAAQTLLQAKASGKLPQVRAVAAAPAQVLSPGKGAPPAPPGKAGPAAIQAQTKGQEEDSESSSEESDSEGEASTAVPHTTSLAQAKPLGKNSQVRAASAASTGPSAKSAVPALPRKARAVAAQVAKWDEDSESSSEEESDSEGEALRAAALAQAKSSGKVLQVRPASGPTQGPPQKAGPAAAQVKTKRSKEDSESSEEESDSEDEAPTAKTPAQAKSAVKTQTKISPRKGTPITPASAKVPPVQVSAPAPWKAGTVTSPTCTSSPAVVRGTQRPEEDSSSSEEPESEEETAPAASVGQVKSVGKSLPVKAASAPTKGPSGQRTTSGLPGKAGPAVAQVKAEVQEDSESSEEESDNEEAASAAAQVKTSVKTPQTKANPAATRVASAKGAPSAPGKGVSAAAQAKLGSQAKVKPPARAPQSSAVSGRGQVSVPAMGKALAAAAQAQLGPVRGPQEDSESSEEESDSEGEAPTQAKPSGKTPQVRTASASTKGSPRKGAGPAPPGKAGAPAAQAGKRKEDSESSSEEESDNDGETPAAVPPAQKEGNSKTARSKTLAPAPTEKKAEGSSTSSDELPARQVIKTPLIFVDPNRSPAGPAATPAQAQATSTPRKAQASESTPRSSSESEDEDVIPATQCSTPAIRTSVATVPTAHPRAALRASMVGAGSSEDTSRVSEGKKQETPATQVTKRNPAHLPLTQAALKVLAQKASEAQPPAARTLSSSGTDSALGTLPMTSPQSTPAQARGANKLRKPEVPATQRAIATPSGRPKAKASGTSDDSKDSSGSSSGSEEDAEGPQVAPLTHRPGPAPSRRETLVEETTADSSDDEMVEPSQSLLSGYVTPGLTPASSQASKATPKPDPNPSVSSTPATRDAPDGKQEVEPQQAAGTMSPKTGRREASATPQKPQKTGKGPRSPQASVLALQSDIARRLLSEPWPLSEAQVQASVVKVLAELLEQEKKKAVDATKEGSRKGRLGNKRKLSEDQTAAGAPRSKKKKRLAAGDGGERAVSPEKAPRTSKGKSKRDKASGDIKEKKEKESPGSQGAKEKPEGEPGKVKGEGGDQGNPKSKKEKKKSDKKKKDKEKKEKKKKAKKASTKDPDSPLQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_007109871.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007109871.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGCGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AAGTTTCCTG 
ACTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC GGAGATTGGC 
CAGAAGCGGA AGGCAGAGGA AGATGTAGCC CTGCAGGCCA AGAAGACCCG CGTGTCAGAC 
CCCATTAGCA GCTCAGAGAG CTCAGAAGAG GAGGAGGAGG AGGAGCAGGG GGCAGAAGCG 
GAAACCACCA AAGCCATCCC GGCACTAGCA TCTACCAACT CCTCAGCCAC GGGGACGGTT 
TTGCCTTCAA GTGTGAAAGA AAAAGCCAAG GCAAAGACCA AGAAAGCCAG CAAGACGGTG 
AACTCCACGG CACACCCTGC CTCCGGGAAG GCAGTGGCCC ACCTCCTCAC CAAGAAGTCA 
CCCCAGAAGG CGGCAGGGCC CTCGGCAAAT ACCATCCTGG TCTCAGAAAC TGAGGAGGAG 
GGCGGTGTCT CGGCCCTCAG AACCACTGCC AAGCCTGGAA TGGCGGCCGC CAGCCAGGCC 
GCCAGCTCCA GCGAGGACAC CTCCAGCTCG AGCGATGAGA CGGACGTGGA GGTGAAACCT 
GCAGTAAAAC CACCACAGGT CAAAGCCTCA GCAGCTGCGG CCAAGGAGTC TCCAAGGAGA 
AGGGCAGCCC CGACCCCAGG GAAGGCGGGA CATGTGACAC CCCAAGTCAG AGGGGGTGCC 
ATGACCCCAG CCAGCAGGGC CAAGAAGCCA GAAGAGATCC CAGAGAGCAG CGAGGAGTCC 
GAGAGTGAGG AGGGGGCCCC TGCGGGGACA CCCAGCCAGG TAAAGGCCTC TGAGAAAACT 
GTCCAGGTCA GAGCTGCCTC GGGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GAGGCCAGAG GAGGACTCGG AGAGCAGCAG CGAGGAGTCG 
GACAGCGAGG AGGAGACGCC AGCTGCCCAG ACCCTGCTGC AGGCAAAGGC CTCAGGGAAG 
CTCCCCCAGG TCAGAGCCGT CGCAGCAGCA CCTGCCCAGG TGCTTTCCCC TGGGAAAGGG 
GCCCCTCCAG CACCCCCTGG CAAGGCAGGG CCTGCAGCCA TCCAGGCCCA GACCAAGGGG 
CAGGAGGAGG ACTCAGAGAG CAGCAGCGAG GAGTCAGACA GCGAGGGGGA GGCGTCCACA 
GCTGTGCCTC ACACCACAAG CCTGGCTCAG GCAAAGCCCT TGGGGAAAAA CTCCCAGGTC 
AGAGCTGCCT CAGCCGCAAG CACGGGGCCC TCAGCGAAGA GCGCTGTCCC AGCGCTCCCT 
CGGAAAGCAA GGGCTGTAGC CGCCCAGGTG GCAAAGTGGG ATGAGGACTC GGAGAGCAGC 
AGCGAGGAGG AGTCGGACAG CGAGGGGGAG GCGCTGAGGG CAGCGGCCTT GGCCCAGGCA 
AAGTCCTCGG GGAAAGTCCT GCAGGTCAGA CCTGCCTCAG GTCCCACCCA GGGCCCCCCT 
CAGAAGGCCG GGCCTGCAGC CGCCCAGGTC AAGACCAAAA GGTCCAAGGA AGACTCGGAG 
AGCAGTGAGG AGGAGTCAGA CAGTGAGGAC GAGGCCCCCA CAGCCAAGAC TCCAGCCCAG 
GCAAAATCAG CTGTGAAAAC TCAGACCAAG ATCTCCCCAA GGAAGGGCAC CCCCATTACA 
CCCGCATCTG CCAAGGTCCC ACCAGTGCAA GTAAGCGCAC CAGCCCCCTG GAAAGCAGGA 
ACGGTGACTT CGCCAACCTG CACATCATCC CCAGCCGTGG TCAGGGGCAC CCAGAGGCCA 
GAGGAAGACT CTTCAAGCAG CGAGGAGCCG GAGAGTGAGG AGGAGACAGC TCCTGCCGCA 
TCCGTGGGAC AGGTGAAGTC TGTAGGGAAA AGCCTCCCTG TGAAAGCTGC CTCGGCACCC 
ACCAAGGGGC CCTCAGGGCA ACGGACCACC TCAGGGCTCC CTGGGAAGGC AGGGCCTGCA 
GTGGCCCAGG TCAAAGCTGA GGTGCAGGAA GACTCAGAGA GCAGTGAGGA GGAGTCGGAC 
AATGAGGAGG CGGCCTCAGC TGCAGCACAG GTGAAGACCT CAGTGAAAAC CCCTCAGACC 
AAAGCCAACC CAGCTGCCAC CAGAGTGGCT TCAGCCAAAG GGGCACCATC AGCTCCTGGA 
AAAGGGGTTT CTGCAGCTGC TCAAGCCAAA CTGGGGTCCC AAGCCAAGGT AAAGCCACCA 
GCAAGAGCCC CCCAGAGCAG TGCCGTCTCG GGCAGGGGCC AAGTGTCTGT GCCGGCTATG 
GGGAAGGCAC TGGCTGCAGC AGCCCAGGCC CAGCTGGGGC CGGTCAGGGG CCCACAGGAG 
GACTCGGAGA GCAGTGAGGA GGAGTCGGAC AGTGAGGGGG AGGCGCCCAC TCAGGCGAAG 
CCCTCAGGGA AGACCCCCCA GGTCAGAACT GCCTCAGCCT CCACCAAGGG GTCCCCCAGG 
AAAGGGGCTG GCCCTGCACC CCCTGGGAAG GCAGGAGCCC CAGCCGCCCA GGCCGGGAAG 
CGGAAAGAGG ACTCGGAGAG CAGCAGCGAG GAGGAGTCAG ACAATGACGG GGAGACGCCG 
GCCGCTGTGC CCCCAGCCCA GAAGGAGGGT AACTCCAAAA CTGCCAGAAG CAAGACCCTG 
GCCCCAGCGC CCACGGAGAA GAAGGCAGAG GGGTCCTCAA CCAGCAGTGA TGAGCTGCCA 
GCGAGACAGG TGATTAAAAC CCCTCTGATT TTTGTCGACC CTAATCGTAG TCCAGCTGGC 
CCAGCTGCTA CCCCCGCCCA AGCCCAGGCT ACAAGCACCC CGAGGAAGGC CCAGGCCTCA 
GAGAGCACAC CCAGGAGCTC CTCTGAGAGT GAGGATGAGG ACGTGATCCC CGCTACGCAG 
TGCTCGACTC CTGCCATTAG GACCAGCGTG GCGACTGTGC CCACGGCCCA CCCCAGGGCA 
GCCCTCAGAG CCAGCATGGT GGGGGCCGGC AGCAGTGAGG ACACCAGTCG GGTGTCTGAA 
GGCAAGAAAC AGGAGACGCC AGCCACTCAG GTGACAAAGA GGAACCCAGC TCACCTCCCG 
CTGACCCAGG CTGCCCTGAA GGTCCTTGCC CAGAAAGCCA GTGAGGCCCA GCCTCCTGCT 
GCCAGGACCC TGTCTTCAAG TGGGACCGAC AGTGCTCTGG GAACACTGCC CATGACGAGT 
CCCCAGAGCA CCCCCGCACA GGCCAGAGGG GCCAACAAGC TCAGAAAGCC TGAGGTTCCT 
GCAACCCAGC GGGCCATAGC CACTCCCTCG GGACGCCCCA AGGCCAAGGC CTCTGGGACC 
TCAGATGACA GCAAGGACAG CAGTGGCAGC TCTTCAGGGA GTGAAGAAGA TGCTGAGGGG 
CCCCAGGTGG CCCCACTGAC CCACAGGCCA GGCCCAGCCC CCTCCAGGAG GGAGACTTTG 
GTGGAAGAGA CCACAGCAGA CTCCAGCGAT GATGAGATGG TGGAACCTTC CCAGTCTCTC 
CTCTCAGGTT ATGTGACCCC TGGGCTGACT CCGGCCAGTT CCCAGGCTTC AAAGGCCACT 
CCCAAGCCAG ACCCCAACCC CTCGGTTTCC TCTACTCCGG CCACCAGAGA TGCCCCGGAC 
GGCAAGCAGG AAGTGGAGCC CCAACAAGCA GCAGGCACCA TGTCCCCTAA AACAGGCAGA 
AGAGAGGCTA GCGCCACACC TCAGAAGCCC CAGAAGACCG GGAAGGGGCC CAGGAGCCCC 
CAGGCCTCCG TGCTGGCGCT GCAGAGCGAC ATCGCCCGGC GCCTGCTGAG CGAGCCCTGG 
CCCCTGAGTG AGGCGCAGGT GCAGGCCTCG GTGGTGAAGG TGTTGGCGGA GCTGCTGGAG 
CAGGAGAAGA AGAAGGCCGT GGATGCCACC AAGGAGGGCA GCCGGAAGGG CCGCCTGGGC 
AACAAGCGGA AGCTCTCCGA AGACCAGACG GCCGCCGGTG CCCCCAGGAG CAAGAAGAAG 
AAGCGGCTGG CAGCCGGGGA TGGCGGGGAG CGTGCAGTCT CCCCAGAAAA GGCCCCCAGG 
ACTTCCAAGG GGAAATCCAA GAGGGACAAA GCGAGTGGTG ACATCAAGGA GAAGAAGGAG 
AAGGAGTCTC CCGGCTCCCA AGGGGCCAAG GAGAAGCCAG AAGGGGAGCC GGGGAAGGTG 
AAGGGTGAGG GGGGAGACCA AGGCAACCCA AAGAGCAAGA AGGAGAAGAA GAAATCCGAC 
AAGAAAAAAA AAGACAAGGA AAAAAAAGAA AAGAAGAAGA AAGCAAAAAA GGCCTCAACC 
AAAGACCCTG ACTCACCATT GCAGAAGAAA AAGAAGAAAA AGAAGAAGAC GGCAGAGCAA 
ACTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_007109872.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007109934.1 Physeter catodon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X2, mRNA.

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CloneID	OPh118879
Clone ID Related Accession (Same CDS sequence)	XM_007109872.1
Accession Version	XM_007109872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	treacle protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006713815.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGCGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AAGTTTCCTG 
ACTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC GGAGATTGGC 
CAGAAGCGGA AGGCAGAGGA AGATGTAGCC CTGCAGGCCA AGAAGACCCG CGTGTCAGAC 
CCCATTAGCA GCTCAGAGAG CTCAGAAGAG GAGGAGGAGG AGGAGCAGGG GGCAGAAGCG 
GAAACCACCA AAGCCATCCC GGCACTAGCA TCTACCAACT CCTCAGCCAC GGGGACGGTT 
TTGCCTTCAA GTGTGAAAGA AAAAGCCAAG GCAAAGACCA AGAAAGCCAG CAAGACGGTG 
AACTCCACGG CACACCCTGC CTCCGGGAAG GCAGTGGCCC ACCTCCTCAC CAAGAAGTCA 
CCCCAGAAGG CGGCAGGGCC CTCGGCAAAT ACCATCCTGG TCTCAGAAAC TGAGGAGGAG 
GGCGGTGTCT CGGCCCTCAG AACCACTGCC AAGCCTGGAA TGGCGGCCGC CAGCCAGGCC 
GCCAGCTCCA GCGAGGACAC CTCCAGCTCG AGCGATGAGA CGGACGTGGA GGTGAAACCT 
GCAGTAAAAC CACCACAGGT CAAAGCCTCA GCAGCTGCGG CCAAGGAGTC TCCAAGGAGA 
AGGGCAGCCC CGACCCCAGG GAAGGCGGGA CATGTGACAC CCCAAGTCAG AGGGGGTGCC 
ATGACCCCAG CCAGCAGGGC CAAGAAGCCA GAAGAGATCC CAGAGAGCAG CGAGGAGTCC 
GAGAGTGAGG AGGGGGCCCC TGCGGGGACA CCCAGCCAGG TAAAGGCCTC TGAGAAAACT 
GTCCAGGTCA GAGCTGCCTC GGGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GAGGCCAGAG GAGGACTCGG AGAGCAGCAG CGAGGAGTCG 
GACAGCGAGG AGGAGACGCC AGCTGCCCAG ACCCTGCTGC AGGCAAAGGC CTCAGGGAAG 
CTCCCCCAGG TCAGAGCCGT CGCAGCAGCA CCTGCCCAGG TGCTTTCCCC TGGGAAAGGG 
GCCCCTCCAG CACCCCCTGG CAAGGCAGGG CCTGCAGCCA TCCAGGCCCA GACCAAGGGG 
CAGGAGGAGG ACTCAGAGAG CAGCAGCGAG GAGTCAGACA GCGAGGGGGA GGCGTCCACA 
GCTGTGCCTC ACACCACAAG CCTGGCTCAG GCAAAGCCCT TGGGGAAAAA CTCCCAGGTC 
AGAGCTGCCT CAGCCGCAAG CACGGGGCCC TCAGCGAAGA GCGCTGTCCC AGCGCTCCCT 
CGGAAAGCAA GGGCTGTAGC CGCCCAGGTG GCAAAGTGGG ATGAGGACTC GGAGAGCAGC 
AGCGAGGAGG AGTCGGACAG CGAGGGGGAG GCGCTGAGGG CAGCGGCCTT GGCCCAGGCA 
AAGTCCTCGG GGAAAGTCCT GCAGGTCAGA CCTGCCTCAG GTCCCACCCA GGGCCCCCCT 
CAGAAGGCCG GGCCTGCAGC CGCCCAGGTC AAGACCAAAA GGTCCAAGGA AGACTCGGAG 
AGCAGTGAGG AGGAGTCAGA CAGTGAGGAC GAGGCCCCCA CAGCCAAGAC TCCAGCCCAG 
GCAAAATCAG CTGTGAAAAC TCAGACCAAG ATCTCCCCAA GGAAGGGCAC CCCCATTACA 
CCCGCATCTG CCAAGGTCCC ACCAGTGCAA GTAAGCGCAC CAGCCCCCTG GAAAGCAGGA 
ACGGTGACTT CGCCAACCTG CACATCATCC CCAGCCGTGG TCAGGGGCAC CCAGAGGCCA 
GAGGAAGACT CTTCAAGCAG CGAGGAGCCG GAGAGTGAGG AGGAGACAGC TCCTGCCGCA 
TCCGTGGGAC AGGTGAAGTC TGTAGGGAAA AGCCTCCCTG TGAAAGCTGC CTCGGCACCC 
ACCAAGGGGC CCTCAGGGCA ACGGACCACC TCAGGGCTCC CTGGGAAGGC AGGGCCTGCA 
GTGGCCCAGG TCAAAGCTGA GGTGCAGGAA GACTCAGAGA GCAGTGAGGA GGAGTCGGAC 
AATGAGGAGG CGGCCTCAGC TGCAGCACAG GTGAAGACCT CAGTGAAAAC CCCTCAGACC 
AAAGCCAACC CAGCTGCCAC CAGAGTGGCT TCAGCCAAAG GGGCACCATC AGCTCCTGGA 
AAAGGGGTTT CTGCAGCTGC TCAAGCCAAA CTGGGGTCCC AAGCCAAGGT AAAGCCACCA 
GCAAGAGCCC CCCAGAGCAG TGCCGTCTCG GGCAGGGGCC AAGTGTCTGT GCCGGCTATG 
GGGAAGGCAC TGGCTGCAGC AGCCCAGGCC CAGCTGGGGC CGGTCAGGGG CCCACAGGAG 
GACTCGGAGA GCAGTGAGGA GGAGTCGGAC AGTGAGGGGG AGGCGCCCAC TCAGGCGAAG 
CCCTCAGGGA AGACCCCCCA GGTCAGAACT GCCTCAGCCT CCACCAAGGG GTCCCCCAGG 
AAAGGGGCTG GCCCTGCACC CCCTGGGAAG GCAGGAGCCC CAGCCGCCCA GGCCGGGAAG 
CGGAAAGAGG ACTCGGAGAG CAGCAGCGAG GAGGAGTCAG ACAATGACGG GGAGACGCCG 
GCCGCTGTGC CCCCAGCCCA GAAGGAGGGT AACTCCAAAA CTGCCAGAAG CAAGACCCTG 
GCCCCAGCGC CCACGGAGAA GAAGGCAGAG GGGTCCTCAA CCAGCAGTGA TGAGCTGCCA 
GCGAGACAGG TGATTAAAAC CCCTCTGATT TTTGTCGACC CTAATCGTAG TCCAGCTGGC 
CCAGCTGCTA CCCCCGCCCA AGCCCAGGCT ACAAGCACCC CGAGGAAGGC CCAGGCCTCA 
GAGAGCACAC CCAGGAGCTC CTCTGAGAGT GAGGATGAGG ACGTGATCCC CGCTACGCAG 
TGCTCGACTC CTGCCATTAG GACCAGCGTG GCGACTGTGC CCACGGCCCA CCCCAGGGCA 
GCCCTCAGAG CCAGCATGGT GGGGGCCGGC AGCAGTGAGG ACACCAGTCG GGTGTCTGAA 
GGCAAGAAAC AGGAGACGCC AGCCACTCAG GTGACAAAGA GGAACCCAGC TCACCTCCCG 
CTGACCCAGG CTGCCCTGAA GGTCCTTGCC CAGAAAGCCA GTGAGGCCCA GCCTCCTGCT 
GCCAGGACCC TGTCTTCAAG TGGGACCGAC AGTGCTCTGG GAACACTGCC CATGACGAGT 
CCCCAGAGCA CCCCCGCACA GGCCAGAGGG GCCAACAAGC TCAGAAAGCC TGAGGTTCCT 
GCAACCCAGC GGGCCATAGC CACTCCCTCG GGACGCCCCA AGGCCAAGGC CTCTGGGACC 
TCAGATGACA GCAAGGACAG CAGTGGCAGC TCTTCAGGGA GTGAAGAAGA TGCTGAGGGG 
CCCCAGGTGG CCCCACTGAC CCACAGGCCA GTAGGCCCAG CCCCCTCCAG GAGGGAGACT 
TTGGTGGAAG AGACCACAGC AGACTCCAGC GATGATGAGA TGGTGGAACC TTCCCAGTCT 
CTCCTCTCAG GTTATGTGAC CCCTGGGCTG ACTCCGGCCA GTTCCCAGGC TTCAAAGGCC 
ACTCCCAAGC CAGACCCCAA CCCCTCGGTT TCCTCTACTC CGGCCACCAG AGATGCCCCG 
GACGGCAAGC AGGAAGTGGA GCCCCAACAA GCAGCAGGCA CCATGTCCCC TAAAACAGGC 
AGAAGAGAGG CTAGCGCCAC ACCTCAGAAG CCCCAGAAGA CCGGGAAGGG GCCCAGGAGC 
CCCCAGGCCT CCGTGCTGGC GCTGCAGAGC GACATCGCCC GGCGCCTGCT GAGCGAGCCC 
TGGCCCCTGA GTGAGGCGCA GGTGCAGGCC TCGGTGGTGA AGGTGTTGGC GGAGCTGCTG 
GAGCAGGAGA AGAAGAAGGC CGTGGATGCC ACCAAGGAGG GCAGCCGGAA GGGCCGCCTG 
GGCAACAAGC GGAAGCTCTC CGAAGACCAG ACGGCCGCCG GTGCCCCCAG GAGCAAGAAG 
AAGAAGCGGC TGGCAGCCGG GGATGGCGGG GAGCGTGCAG TCTCCCCAGA AAAGGCCCCC 
AGGACTTCCA AGGGGAAATC CAAGAGGGAC AAAGCGAGTG GTGACATCAA GGAGAAGAAG 
GAGAAGGAGT CTCCCGGCTC CCAAGGGGCC AAGGAGAAGC CAGAAGGGGA GCCGGGGAAG 
GTGAAGGGTG AGGGGGGAGA CCAAGGCAAC CCAAAGAGCA AGAAGGAGAA GAAGAAATCC 
GACAAGAAAA AAAAAGACAA GGAAAAAAAA GAAAAGAAGA AGAAAGCAAA AAAGGCCTCA 
ACCAAAGACC CTGACTCACC ATTGCAGAAG AAAAAGAAGA AAAAGAAGAA GACGGCAGAG 
CAAACTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007109934.1
CDS	1..4392
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKSFLTQPVTLLDIYTHWQQTSEIGQKRKAEEDVALQAKKTRVSDPISSSESSEEEEEEEQGAEAETTKAIPALASTNSSATGTVLPSSVKEKAKAKTKKASKTVNSTAHPASGKAVAHLLTKKSPQKAAGPSANTILVSETEEEGGVSALRTTAKPGMAAASQAASSSEDTSSSSDETDVEVKPAVKPPQVKASAAAAKESPRRRAAPTPGKAGHVTPQVRGGAMTPASRAKKPEEIPESSEESESEEGAPAGTPSQVKASEKTVQVRAASGPAKGTPGKGATPAPPGKAGRPEEDSESSSEESDSEEETPAAQTLLQAKASGKLPQVRAVAAAPAQVLSPGKGAPPAPPGKAGPAAIQAQTKGQEEDSESSSEESDSEGEASTAVPHTTSLAQAKPLGKNSQVRAASAASTGPSAKSAVPALPRKARAVAAQVAKWDEDSESSSEEESDSEGEALRAAALAQAKSSGKVLQVRPASGPTQGPPQKAGPAAAQVKTKRSKEDSESSEEESDSEDEAPTAKTPAQAKSAVKTQTKISPRKGTPITPASAKVPPVQVSAPAPWKAGTVTSPTCTSSPAVVRGTQRPEEDSSSSEEPESEEETAPAASVGQVKSVGKSLPVKAASAPTKGPSGQRTTSGLPGKAGPAVAQVKAEVQEDSESSEEESDNEEAASAAAQVKTSVKTPQTKANPAATRVASAKGAPSAPGKGVSAAAQAKLGSQAKVKPPARAPQSSAVSGRGQVSVPAMGKALAAAAQAQLGPVRGPQEDSESSEEESDSEGEAPTQAKPSGKTPQVRTASASTKGSPRKGAGPAPPGKAGAPAAQAGKRKEDSESSSEEESDNDGETPAAVPPAQKEGNSKTARSKTLAPAPTEKKAEGSSTSSDELPARQVIKTPLIFVDPNRSPAGPAATPAQAQATSTPRKAQASESTPRSSSESEDEDVIPATQCSTPAIRTSVATVPTAHPRAALRASMVGAGSSEDTSRVSEGKKQETPATQVTKRNPAHLPLTQAALKVLAQKASEAQPPAARTLSSSGTDSALGTLPMTSPQSTPAQARGANKLRKPEVPATQRAIATPSGRPKAKASGTSDDSKDSSGSSSGSEEDAEGPQVAPLTHRPVGPAPSRRETLVEETTADSSDDEMVEPSQSLLSGYVTPGLTPASSQASKATPKPDPNPSVSSTPATRDAPDGKQEVEPQQAAGTMSPKTGRREASATPQKPQKTGKGPRSPQASVLALQSDIARRLLSEPWPLSEAQVQASVVKVLAELLEQEKKKAVDATKEGSRKGRLGNKRKLSEDQTAAGAPRSKKKKRLAAGDGGERAVSPEKAPRTSKGKSKRDKASGDIKEKKEKESPGSQGAKEKPEGEPGKVKGEGGDQGNPKSKKEKKKSDKKKKDKEKKEKKKKAKKASTKDPDSPLQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_007109872.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007109872.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGCGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AAGTTTCCTG 
ACTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC GGAGATTGGC 
CAGAAGCGGA AGGCAGAGGA AGATGTAGCC CTGCAGGCCA AGAAGACCCG CGTGTCAGAC 
CCCATTAGCA GCTCAGAGAG CTCAGAAGAG GAGGAGGAGG AGGAGCAGGG GGCAGAAGCG 
GAAACCACCA AAGCCATCCC GGCACTAGCA TCTACCAACT CCTCAGCCAC GGGGACGGTT 
TTGCCTTCAA GTGTGAAAGA AAAAGCCAAG GCAAAGACCA AGAAAGCCAG CAAGACGGTG 
AACTCCACGG CACACCCTGC CTCCGGGAAG GCAGTGGCCC ACCTCCTCAC CAAGAAGTCA 
CCCCAGAAGG CGGCAGGGCC CTCGGCAAAT ACCATCCTGG TCTCAGAAAC TGAGGAGGAG 
GGCGGTGTCT CGGCCCTCAG AACCACTGCC AAGCCTGGAA TGGCGGCCGC CAGCCAGGCC 
GCCAGCTCCA GCGAGGACAC CTCCAGCTCG AGCGATGAGA CGGACGTGGA GGTGAAACCT 
GCAGTAAAAC CACCACAGGT CAAAGCCTCA GCAGCTGCGG CCAAGGAGTC TCCAAGGAGA 
AGGGCAGCCC CGACCCCAGG GAAGGCGGGA CATGTGACAC CCCAAGTCAG AGGGGGTGCC 
ATGACCCCAG CCAGCAGGGC CAAGAAGCCA GAAGAGATCC CAGAGAGCAG CGAGGAGTCC 
GAGAGTGAGG AGGGGGCCCC TGCGGGGACA CCCAGCCAGG TAAAGGCCTC TGAGAAAACT 
GTCCAGGTCA GAGCTGCCTC GGGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GAGGCCAGAG GAGGACTCGG AGAGCAGCAG CGAGGAGTCG 
GACAGCGAGG AGGAGACGCC AGCTGCCCAG ACCCTGCTGC AGGCAAAGGC CTCAGGGAAG 
CTCCCCCAGG TCAGAGCCGT CGCAGCAGCA CCTGCCCAGG TGCTTTCCCC TGGGAAAGGG 
GCCCCTCCAG CACCCCCTGG CAAGGCAGGG CCTGCAGCCA TCCAGGCCCA GACCAAGGGG 
CAGGAGGAGG ACTCAGAGAG CAGCAGCGAG GAGTCAGACA GCGAGGGGGA GGCGTCCACA 
GCTGTGCCTC ACACCACAAG CCTGGCTCAG GCAAAGCCCT TGGGGAAAAA CTCCCAGGTC 
AGAGCTGCCT CAGCCGCAAG CACGGGGCCC TCAGCGAAGA GCGCTGTCCC AGCGCTCCCT 
CGGAAAGCAA GGGCTGTAGC CGCCCAGGTG GCAAAGTGGG ATGAGGACTC GGAGAGCAGC 
AGCGAGGAGG AGTCGGACAG CGAGGGGGAG GCGCTGAGGG CAGCGGCCTT GGCCCAGGCA 
AAGTCCTCGG GGAAAGTCCT GCAGGTCAGA CCTGCCTCAG GTCCCACCCA GGGCCCCCCT 
CAGAAGGCCG GGCCTGCAGC CGCCCAGGTC AAGACCAAAA GGTCCAAGGA AGACTCGGAG 
AGCAGTGAGG AGGAGTCAGA CAGTGAGGAC GAGGCCCCCA CAGCCAAGAC TCCAGCCCAG 
GCAAAATCAG CTGTGAAAAC TCAGACCAAG ATCTCCCCAA GGAAGGGCAC CCCCATTACA 
CCCGCATCTG CCAAGGTCCC ACCAGTGCAA GTAAGCGCAC CAGCCCCCTG GAAAGCAGGA 
ACGGTGACTT CGCCAACCTG CACATCATCC CCAGCCGTGG TCAGGGGCAC CCAGAGGCCA 
GAGGAAGACT CTTCAAGCAG CGAGGAGCCG GAGAGTGAGG AGGAGACAGC TCCTGCCGCA 
TCCGTGGGAC AGGTGAAGTC TGTAGGGAAA AGCCTCCCTG TGAAAGCTGC CTCGGCACCC 
ACCAAGGGGC CCTCAGGGCA ACGGACCACC TCAGGGCTCC CTGGGAAGGC AGGGCCTGCA 
GTGGCCCAGG TCAAAGCTGA GGTGCAGGAA GACTCAGAGA GCAGTGAGGA GGAGTCGGAC 
AATGAGGAGG CGGCCTCAGC TGCAGCACAG GTGAAGACCT CAGTGAAAAC CCCTCAGACC 
AAAGCCAACC CAGCTGCCAC CAGAGTGGCT TCAGCCAAAG GGGCACCATC AGCTCCTGGA 
AAAGGGGTTT CTGCAGCTGC TCAAGCCAAA CTGGGGTCCC AAGCCAAGGT AAAGCCACCA 
GCAAGAGCCC CCCAGAGCAG TGCCGTCTCG GGCAGGGGCC AAGTGTCTGT GCCGGCTATG 
GGGAAGGCAC TGGCTGCAGC AGCCCAGGCC CAGCTGGGGC CGGTCAGGGG CCCACAGGAG 
GACTCGGAGA GCAGTGAGGA GGAGTCGGAC AGTGAGGGGG AGGCGCCCAC TCAGGCGAAG 
CCCTCAGGGA AGACCCCCCA GGTCAGAACT GCCTCAGCCT CCACCAAGGG GTCCCCCAGG 
AAAGGGGCTG GCCCTGCACC CCCTGGGAAG GCAGGAGCCC CAGCCGCCCA GGCCGGGAAG 
CGGAAAGAGG ACTCGGAGAG CAGCAGCGAG GAGGAGTCAG ACAATGACGG GGAGACGCCG 
GCCGCTGTGC CCCCAGCCCA GAAGGAGGGT AACTCCAAAA CTGCCAGAAG CAAGACCCTG 
GCCCCAGCGC CCACGGAGAA GAAGGCAGAG GGGTCCTCAA CCAGCAGTGA TGAGCTGCCA 
GCGAGACAGG TGATTAAAAC CCCTCTGATT TTTGTCGACC CTAATCGTAG TCCAGCTGGC 
CCAGCTGCTA CCCCCGCCCA AGCCCAGGCT ACAAGCACCC CGAGGAAGGC CCAGGCCTCA 
GAGAGCACAC CCAGGAGCTC CTCTGAGAGT GAGGATGAGG ACGTGATCCC CGCTACGCAG 
TGCTCGACTC CTGCCATTAG GACCAGCGTG GCGACTGTGC CCACGGCCCA CCCCAGGGCA 
GCCCTCAGAG CCAGCATGGT GGGGGCCGGC AGCAGTGAGG ACACCAGTCG GGTGTCTGAA 
GGCAAGAAAC AGGAGACGCC AGCCACTCAG GTGACAAAGA GGAACCCAGC TCACCTCCCG 
CTGACCCAGG CTGCCCTGAA GGTCCTTGCC CAGAAAGCCA GTGAGGCCCA GCCTCCTGCT 
GCCAGGACCC TGTCTTCAAG TGGGACCGAC AGTGCTCTGG GAACACTGCC CATGACGAGT 
CCCCAGAGCA CCCCCGCACA GGCCAGAGGG GCCAACAAGC TCAGAAAGCC TGAGGTTCCT 
GCAACCCAGC GGGCCATAGC CACTCCCTCG GGACGCCCCA AGGCCAAGGC CTCTGGGACC 
TCAGATGACA GCAAGGACAG CAGTGGCAGC TCTTCAGGGA GTGAAGAAGA TGCTGAGGGG 
CCCCAGGTGG CCCCACTGAC CCACAGGCCA GTAGGCCCAG CCCCCTCCAG GAGGGAGACT 
TTGGTGGAAG AGACCACAGC AGACTCCAGC GATGATGAGA TGGTGGAACC TTCCCAGTCT 
CTCCTCTCAG GTTATGTGAC CCCTGGGCTG ACTCCGGCCA GTTCCCAGGC TTCAAAGGCC 
ACTCCCAAGC CAGACCCCAA CCCCTCGGTT TCCTCTACTC CGGCCACCAG AGATGCCCCG 
GACGGCAAGC AGGAAGTGGA GCCCCAACAA GCAGCAGGCA CCATGTCCCC TAAAACAGGC 
AGAAGAGAGG CTAGCGCCAC ACCTCAGAAG CCCCAGAAGA CCGGGAAGGG GCCCAGGAGC 
CCCCAGGCCT CCGTGCTGGC GCTGCAGAGC GACATCGCCC GGCGCCTGCT GAGCGAGCCC 
TGGCCCCTGA GTGAGGCGCA GGTGCAGGCC TCGGTGGTGA AGGTGTTGGC GGAGCTGCTG 
GAGCAGGAGA AGAAGAAGGC CGTGGATGCC ACCAAGGAGG GCAGCCGGAA GGGCCGCCTG 
GGCAACAAGC GGAAGCTCTC CGAAGACCAG ACGGCCGCCG GTGCCCCCAG GAGCAAGAAG 
AAGAAGCGGC TGGCAGCCGG GGATGGCGGG GAGCGTGCAG TCTCCCCAGA AAAGGCCCCC 
AGGACTTCCA AGGGGAAATC CAAGAGGGAC AAAGCGAGTG GTGACATCAA GGAGAAGAAG 
GAGAAGGAGT CTCCCGGCTC CCAAGGGGCC AAGGAGAAGC CAGAAGGGGA GCCGGGGAAG 
GTGAAGGGTG AGGGGGGAGA CCAAGGCAAC CCAAAGAGCA AGAAGGAGAA GAAGAAATCC 
GACAAGAAAA AAAAAGACAA GGAAAAAAAA GAAAAGAAGA AGAAAGCAAA AAAGGCCTCA 
ACCAAAGACC CTGACTCACC ATTGCAGAAG AAAAAGAAGA AAAAGAAGAA GACGGCAGAG 
CAAACTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_024124753.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_023980521.1 Physeter catodon treacle ribosome biogenesis factor 1 (TCOF1), mRNA.

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CloneID	OPh284670
Clone ID Related Accession (Same CDS sequence)	XM_024124753.1
Accession Version	XM_024124753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	treacle protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGCGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AAGTTTCCTG 
ACTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC GGAGATTGGC 
CAGAAGCGGA AGGCAGAGGA AGATGTAGCC CTGCAGGCCA AGAAGACCCG CGTGTCAGAC 
CCCATTAGCA GCTCAGAGAG CTCAGAAGAG GAGGAGGAGG AGGAGCAGGG GGCAGAAGCG 
GAAACCACCA AAGCCATCCC GGCACTAGCA TCTACCAACT CCTCAGCCAC GGGGACGGTT 
TTGCCTTCAA GTGTGAAAGA AAAAGCCAAG GCAAAGACCA AGAAAGCCAG CAAGACGGTG 
AACTCCACGG CACACCCTGC CTCCGGGAAG GCAGTGGCCC ACCTCCTCAC CAAGAAGTCA 
CCCCAGAAGG CGGCAGGGCC CTCGGCAAAT ACCATCCTGG TCTCAGAAAC TGAGGAGGAG 
GGCGGTGTCT CGGCCCTCAG AACCACTGCC AAGCCTGGAA TGGCGGCCGC CAGCCAGGCC 
GCCAGCTCCA GCGAGGACAC CTCCAGCTCG AGCGATGAGA CGGACGTGGA GGTGAAACCT 
GCAGTAAAAC CACCACAGGT CAAAGCCTCA GCAGCTGCGG CCAAGGAGTC TCCAAGGAGA 
AGGGCAGCCC CGACCCCAGG GAAGGCGGGA CATGTGACAC CCCAAGTCAG AGGGGGTGCC 
ATGACCCCAG CCAGCAGGGC CAAGAAGCCA GAAGAGATCC CAGAGAGCAG CGAGGAGTCC 
GAGAGTGAGG AGGGGGCCCC TGCGGGGACA CCCAGCCAGG TAAAGGCCTC TGAGAAAACT 
GTCCAGGTCA GAGCTGCCTC GGGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GAGGCCAGAG GAGGACTCGG AGAGCAGCAG CGAGGAGTCG 
GACAGCGAGG AGGAGACGCC AGCTGCCCAG ACCCTGCTGC AGGCAAAGGC CTCAGGGAAG 
CTCCCCCAGG TCAGAGCCGT CGCAGCAGCA CCTGCCCAGG TGCTTTCCCC TGGGAAAGGG 
GCCCCTCCAG CACCCCCTGG CAAGGCAGGG CCTGCAGCCA TCCAGGCCCA GACCAAGGGG 
CAGGAGGAGG ACTCAGAGAG CAGCAGCGAG GAGTCAGACA GCGAGGGGGA GGCGTCCACA 
GCTGTGCCTC ACACCACAAG CCTGGCTCAG GCAAAGCCCT TGGGGAAAAA CTCCCAGGTC 
AGAGCTGCCT CAGCCGCAAG CACGGGGCCC TCAGCGAAGA GCGCTGTCCC AGCGCTCCCT 
CGGAAAGCAA GGGCTGTAGC CGCCCAGGTG GCAAAGTGGG ATGAGGACTC GGAGAGCAGC 
AGCGAGGAGG AGTCGGACAG CGAGGGGGAG GCGCTGAGGG CAGCGGCCTT GGCCCAGGCA 
AAGTCCTCGG GGAAAGTCCT GCAGGTCAGA CCTGCCTCAG GTCCCACCCA GGGCCCCCCT 
CAGAAGGCCG GGCCTGCAGC CGCCCAGGTC AAGACCAAAA GGTCCAAGGA AGACTCGGAG 
AGCAGTGAGG AGGAGTCAGA CAGTGAGGAC GAGGCCCCCA CAGCCAAGAC TCCAGCCCAG 
GCAAAATCAG CTGTGAAAAC TCAGACCAAG ATCTCCCCAA GGAAGGGCAC CCCCATTACA 
CCCGCATCTG CCAAGGTCCC ACCAGTGCAA GTAAGCGCAC CAGCCCCCTG GAAAGCAGGA 
ACGGTGACTT CGCCAACCTG CACATCATCC CCAGCCGTGG TCAGGGGCAC CCAGAGGCCA 
GAGGAAGACT CTTCAAGCAG CGAGGAGCCG GAGAGTGAGG AGGAGACAGC TCCTGCCGCA 
TCCGTGGGAC AGGTGAAGTC TGTAGGGAAA AGCCTCCCTG TGAAAGCTGC CTCGGCACCC 
ACCAAGGGGC CCTCAGGGCA ACGGACCACC TCAGGGCTCC CTGGGAAGGC AGGGCCTGCA 
GTGGCCCAGG TCAAAGCTGA GGTGCAGGAA GACTCAGAGA GCAGTGAGGA GGAGTCGGAC 
AATGAGGAGG CGGCCTCAGC TGCAGCACAG GTGAAGACCT CAGTGAAAAC CCCTCAGACC 
AAAGCCAACC CAGCTGCCAC CAGAGTGGCT TCAGCCAAAG GGGCACCATC AGCTCCTGGA 
AAAGGGGTTT CTGCAGCTGC TCAAGCCAAA CTGGGGTCCC AAGCCAAGGT AAAGCCACCA 
GCAAGAGCCC CCCAGAGCAG TGCCGTCTCG GGCAGGGGCC AAGTGTCTGT GCCGGCTATG 
GGGAAGGCAC TGGCTGCAGC AGCCCAGGCC CAGCTGGGGC CGGTCAGGGG CCCACAGGAG 
GACTCGGAGA GCAGTGAGGA GGAGTCGGAC AGTGAGGGGG AGGCGCCCAC TCAGGCGAAG 
CCCTCAGGGA AGACCCCCCA GGTCAGAACT GCCTCAGCCT CCACCAAGGG GTCCCCCAGG 
AAAGGGGCTG GCCCTGCACC CCCTGGGAAG GCAGGAGCCC CAGCCGCCCA GGCCGGGAAG 
CGGAAAGAGG ACTCGGAGAG CAGCAGCGAG GAGGAGTCAG ACAATGACGG GGAGACGCCG 
GCCGCTGTGC CCCCAGCCCA GAAGGAGGGT AACTCCAAAA CTGCCAGAAG CAAGACCCTG 
GCCCCAGCGC CCACGGAGAA GAAGGCAGAG GGGTCCTCAA CCAGCAGTGA TGAGCTGCCA 
GCGAGACAGG TGATTAAAAC CCCTCTGATT TTTGTCGACC CTAATCGTAG TCCAGCTGGC 
CCAGCTGCTA CCCCCGCCCA AGCCCAGGCT ACAAGCACCC CGAGGAAGGC CCAGGCCTCA 
GAGAGCACAC CCAGGAGCTC CTCTGAGAGT GAGGATGAGG ACGTGACCCC CGCTACGCAG 
TGCTCGACTC CTGCCATTAG GACCAGCGTG GCGACTGTGC CCACGGCCCA CCCCAGGGCA 
GCCCTCAGAG CCAGCATGGT GGGGGCCGGC AGCAGTGAGG ACACCAGTCG GGTGTCTGAA 
GGCAAGAAAC AGGAGACGCC AGCCACTCAG GTGACAAAGA GGAACCCAGC TCACCTCCCG 
CTGACCCAGG CTGCCCTGAA GGTCCTTGCC CAGAAAGCCA GTGAGGCCCA GCCTCCTGCT 
GCCAGGACCC TGTCTTCAAG TGGGACCGAC AGTGCTCTGG GAACACTGCC CATGACGAGT 
CCCCAGAGCA CCCCCGCACA GGCCAGAGGG GCCAACAAGC TCAGAAAGCC TGAGGTTCCT 
GCAACCCAGC GGGCCATAGC CACTCCCTCG GGATGCCCCA AGGCCAAGGC CTCTGGGACC 
TCAGATGACA GCAAGGACAG CAGTGGCAGC TCTTCAGGGA GTGAAGAAGA TGCTGAGGGG 
CCCCAGGTGG CCCCACTGAC CCACAGGCCA GTAGGCCCAG CCCCCTCCAG GAGGGAGACT 
TTGGTGGAAG AGACCACAGC AGACTCCAGC GATGATGAGA TGGTGGAACC TTCCCAGTCT 
CTCCTCTCAG GTTATGTGAC CCCTGGGCTG ACTCCGGCCA GTTCCCAGGC TTCAAAGGCC 
ACTCCCAAGC CAGACCCCAA CCCCTCGGTT TCCTCTACTC CGGCCACCAG AGATGCCCCG 
GACGGCAAGC AGAAAGTGGA GCCCCAACAA GCAGCAGGCA CCATGTCCCC TAAAACAGGC 
AGAAGAGAGG CTAGCGCCAC ACCTCAGAAG CCCCAGAAGA CCGGGAAGGG GCCCAGGAGC 
CCCCAGGCCT CCGTGCTGGC GCTGCAGAGC GACATCGCCC GGCGCCTGCT GAGCGAGCCC 
TGGCCCCTGA GTGAGGCGCA GGTGCAGGCC TCGGTGGTGA AGGTGTTGGC GGAGCTGCTG 
GAGCAGGAGA AGAAGAAGGC CGTGGATGCC ACCAAGGAGG GCAGCCGGAA GGGCCGCCTG 
GGCAACAAGC GGAAGCTCTC CGAAGACCAG ACGGCCGCCG GTGCCCCCAG GAGCAAGAAG 
AAGAAGCGGC TGGCAGCCGG GGATGGCGGG GAGCGTGCAG TCTCCCCAGA AAAGGCCCCC 
AGGACTTCCA AGGGGAAATC CAAGAGGGAC AAAGCGAGTG GTGACATCAA GGAGAAGAAG 
GAGAAGGAGT CTCCCGGCTC CCAAGGGGCC AAGGAGAAGC CAGAAGGGGA GCCGGGGAAG 
GTGAAGGGTG AGGGGGGAGA CCAAGGCAAC CCAAAGAGCA AGAAGGAGAA GAAGAAATCC 
GACAAGAGTA AGTGCCCGGT GCAGCCCCGG AGCGCGTTCC CCAGCCCCGC CGAGCGCTGT 
CGGTCACCTC CCGGGCAGGC AGCCCTCAGC CAGCACAGAG ATGGGGCTGA GAGTGGCTTG 
TCCATCATGG GGGGGCAGGA TGGGCAAAGC CAGAACCAGG TGCCGGAGCA CAGGGGCCAC 
CACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023980521.1
CDS	1..4449
Translation	MAEARKRRELLPLIYQHLLQAGYVRAAREVKEQSGQKSFLTQPVTLLDIYTHWQQTSEIGQKRKAEEDVALQAKKTRVSDPISSSESSEEEEEEEQGAEAETTKAIPALASTNSSATGTVLPSSVKEKAKAKTKKASKTVNSTAHPASGKAVAHLLTKKSPQKAAGPSANTILVSETEEEGGVSALRTTAKPGMAAASQAASSSEDTSSSSDETDVEVKPAVKPPQVKASAAAAKESPRRRAAPTPGKAGHVTPQVRGGAMTPASRAKKPEEIPESSEESESEEGAPAGTPSQVKASEKTVQVRAASGPAKGTPGKGATPAPPGKAGRPEEDSESSSEESDSEEETPAAQTLLQAKASGKLPQVRAVAAAPAQVLSPGKGAPPAPPGKAGPAAIQAQTKGQEEDSESSSEESDSEGEASTAVPHTTSLAQAKPLGKNSQVRAASAASTGPSAKSAVPALPRKARAVAAQVAKWDEDSESSSEEESDSEGEALRAAALAQAKSSGKVLQVRPASGPTQGPPQKAGPAAAQVKTKRSKEDSESSEEESDSEDEAPTAKTPAQAKSAVKTQTKISPRKGTPITPASAKVPPVQVSAPAPWKAGTVTSPTCTSSPAVVRGTQRPEEDSSSSEEPESEEETAPAASVGQVKSVGKSLPVKAASAPTKGPSGQRTTSGLPGKAGPAVAQVKAEVQEDSESSEEESDNEEAASAAAQVKTSVKTPQTKANPAATRVASAKGAPSAPGKGVSAAAQAKLGSQAKVKPPARAPQSSAVSGRGQVSVPAMGKALAAAAQAQLGPVRGPQEDSESSEEESDSEGEAPTQAKPSGKTPQVRTASASTKGSPRKGAGPAPPGKAGAPAAQAGKRKEDSESSSEEESDNDGETPAAVPPAQKEGNSKTARSKTLAPAPTEKKAEGSSTSSDELPARQVIKTPLIFVDPNRSPAGPAATPAQAQATSTPRKAQASESTPRSSSESEDEDVTPATQCSTPAIRTSVATVPTAHPRAALRASMVGAGSSEDTSRVSEGKKQETPATQVTKRNPAHLPLTQAALKVLAQKASEAQPPAARTLSSSGTDSALGTLPMTSPQSTPAQARGANKLRKPEVPATQRAIATPSGCPKAKASGTSDDSKDSSGSSSGSEEDAEGPQVAPLTHRPVGPAPSRRETLVEETTADSSDDEMVEPSQSLLSGYVTPGLTPASSQASKATPKPDPNPSVSSTPATRDAPDGKQKVEPQQAAGTMSPKTGRREASATPQKPQKTGKGPRSPQASVLALQSDIARRLLSEPWPLSEAQVQASVVKVLAELLEQEKKKAVDATKEGSRKGRLGNKRKLSEDQTAAGAPRSKKKKRLAAGDGGERAVSPEKAPRTSKGKSKRDKASGDIKEKKEKESPGSQGAKEKPEGEPGKVKGEGGDQGNPKSKKEKKKSDKSKCPVQPRSAFPSPAERCRSPPGQAALSQHRDGAESGLSIMGGQDGQSQNQVPEHRGHHC

Target ORF information:

RefSeq Version	XM_024124753.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon treacle ribosome biogenesis factor 1 (TCOF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024124753.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTG CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
GCGGGCTATG TGCGCGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AAGTTTCCTG 
ACTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC GGAGATTGGC 
CAGAAGCGGA AGGCAGAGGA AGATGTAGCC CTGCAGGCCA AGAAGACCCG CGTGTCAGAC 
CCCATTAGCA GCTCAGAGAG CTCAGAAGAG GAGGAGGAGG AGGAGCAGGG GGCAGAAGCG 
GAAACCACCA AAGCCATCCC GGCACTAGCA TCTACCAACT CCTCAGCCAC GGGGACGGTT 
TTGCCTTCAA GTGTGAAAGA AAAAGCCAAG GCAAAGACCA AGAAAGCCAG CAAGACGGTG 
AACTCCACGG CACACCCTGC CTCCGGGAAG GCAGTGGCCC ACCTCCTCAC CAAGAAGTCA 
CCCCAGAAGG CGGCAGGGCC CTCGGCAAAT ACCATCCTGG TCTCAGAAAC TGAGGAGGAG 
GGCGGTGTCT CGGCCCTCAG AACCACTGCC AAGCCTGGAA TGGCGGCCGC CAGCCAGGCC 
GCCAGCTCCA GCGAGGACAC CTCCAGCTCG AGCGATGAGA CGGACGTGGA GGTGAAACCT 
GCAGTAAAAC CACCACAGGT CAAAGCCTCA GCAGCTGCGG CCAAGGAGTC TCCAAGGAGA 
AGGGCAGCCC CGACCCCAGG GAAGGCGGGA CATGTGACAC CCCAAGTCAG AGGGGGTGCC 
ATGACCCCAG CCAGCAGGGC CAAGAAGCCA GAAGAGATCC CAGAGAGCAG CGAGGAGTCC 
GAGAGTGAGG AGGGGGCCCC TGCGGGGACA CCCAGCCAGG TAAAGGCCTC TGAGAAAACT 
GTCCAGGTCA GAGCTGCCTC GGGTCCTGCC AAGGGGACCC CTGGGAAAGG GGCCACCCCA 
GCACCCCCTG GGAAGGCAGG GAGGCCAGAG GAGGACTCGG AGAGCAGCAG CGAGGAGTCG 
GACAGCGAGG AGGAGACGCC AGCTGCCCAG ACCCTGCTGC AGGCAAAGGC CTCAGGGAAG 
CTCCCCCAGG TCAGAGCCGT CGCAGCAGCA CCTGCCCAGG TGCTTTCCCC TGGGAAAGGG 
GCCCCTCCAG CACCCCCTGG CAAGGCAGGG CCTGCAGCCA TCCAGGCCCA GACCAAGGGG 
CAGGAGGAGG ACTCAGAGAG CAGCAGCGAG GAGTCAGACA GCGAGGGGGA GGCGTCCACA 
GCTGTGCCTC ACACCACAAG CCTGGCTCAG GCAAAGCCCT TGGGGAAAAA CTCCCAGGTC 
AGAGCTGCCT CAGCCGCAAG CACGGGGCCC TCAGCGAAGA GCGCTGTCCC AGCGCTCCCT 
CGGAAAGCAA GGGCTGTAGC CGCCCAGGTG GCAAAGTGGG ATGAGGACTC GGAGAGCAGC 
AGCGAGGAGG AGTCGGACAG CGAGGGGGAG GCGCTGAGGG CAGCGGCCTT GGCCCAGGCA 
AAGTCCTCGG GGAAAGTCCT GCAGGTCAGA CCTGCCTCAG GTCCCACCCA GGGCCCCCCT 
CAGAAGGCCG GGCCTGCAGC CGCCCAGGTC AAGACCAAAA GGTCCAAGGA AGACTCGGAG 
AGCAGTGAGG AGGAGTCAGA CAGTGAGGAC GAGGCCCCCA CAGCCAAGAC TCCAGCCCAG 
GCAAAATCAG CTGTGAAAAC TCAGACCAAG ATCTCCCCAA GGAAGGGCAC CCCCATTACA 
CCCGCATCTG CCAAGGTCCC ACCAGTGCAA GTAAGCGCAC CAGCCCCCTG GAAAGCAGGA 
ACGGTGACTT CGCCAACCTG CACATCATCC CCAGCCGTGG TCAGGGGCAC CCAGAGGCCA 
GAGGAAGACT CTTCAAGCAG CGAGGAGCCG GAGAGTGAGG AGGAGACAGC TCCTGCCGCA 
TCCGTGGGAC AGGTGAAGTC TGTAGGGAAA AGCCTCCCTG TGAAAGCTGC CTCGGCACCC 
ACCAAGGGGC CCTCAGGGCA ACGGACCACC TCAGGGCTCC CTGGGAAGGC AGGGCCTGCA 
GTGGCCCAGG TCAAAGCTGA GGTGCAGGAA GACTCAGAGA GCAGTGAGGA GGAGTCGGAC 
AATGAGGAGG CGGCCTCAGC TGCAGCACAG GTGAAGACCT CAGTGAAAAC CCCTCAGACC 
AAAGCCAACC CAGCTGCCAC CAGAGTGGCT TCAGCCAAAG GGGCACCATC AGCTCCTGGA 
AAAGGGGTTT CTGCAGCTGC TCAAGCCAAA CTGGGGTCCC AAGCCAAGGT AAAGCCACCA 
GCAAGAGCCC CCCAGAGCAG TGCCGTCTCG GGCAGGGGCC AAGTGTCTGT GCCGGCTATG 
GGGAAGGCAC TGGCTGCAGC AGCCCAGGCC CAGCTGGGGC CGGTCAGGGG CCCACAGGAG 
GACTCGGAGA GCAGTGAGGA GGAGTCGGAC AGTGAGGGGG AGGCGCCCAC TCAGGCGAAG 
CCCTCAGGGA AGACCCCCCA GGTCAGAACT GCCTCAGCCT CCACCAAGGG GTCCCCCAGG 
AAAGGGGCTG GCCCTGCACC CCCTGGGAAG GCAGGAGCCC CAGCCGCCCA GGCCGGGAAG 
CGGAAAGAGG ACTCGGAGAG CAGCAGCGAG GAGGAGTCAG ACAATGACGG GGAGACGCCG 
GCCGCTGTGC CCCCAGCCCA GAAGGAGGGT AACTCCAAAA CTGCCAGAAG CAAGACCCTG 
GCCCCAGCGC CCACGGAGAA GAAGGCAGAG GGGTCCTCAA CCAGCAGTGA TGAGCTGCCA 
GCGAGACAGG TGATTAAAAC CCCTCTGATT TTTGTCGACC CTAATCGTAG TCCAGCTGGC 
CCAGCTGCTA CCCCCGCCCA AGCCCAGGCT ACAAGCACCC CGAGGAAGGC CCAGGCCTCA 
GAGAGCACAC CCAGGAGCTC CTCTGAGAGT GAGGATGAGG ACGTGACCCC CGCTACGCAG 
TGCTCGACTC CTGCCATTAG GACCAGCGTG GCGACTGTGC CCACGGCCCA CCCCAGGGCA 
GCCCTCAGAG CCAGCATGGT GGGGGCCGGC AGCAGTGAGG ACACCAGTCG GGTGTCTGAA 
GGCAAGAAAC AGGAGACGCC AGCCACTCAG GTGACAAAGA GGAACCCAGC TCACCTCCCG 
CTGACCCAGG CTGCCCTGAA GGTCCTTGCC CAGAAAGCCA GTGAGGCCCA GCCTCCTGCT 
GCCAGGACCC TGTCTTCAAG TGGGACCGAC AGTGCTCTGG GAACACTGCC CATGACGAGT 
CCCCAGAGCA CCCCCGCACA GGCCAGAGGG GCCAACAAGC TCAGAAAGCC TGAGGTTCCT 
GCAACCCAGC GGGCCATAGC CACTCCCTCG GGATGCCCCA AGGCCAAGGC CTCTGGGACC 
TCAGATGACA GCAAGGACAG CAGTGGCAGC TCTTCAGGGA GTGAAGAAGA TGCTGAGGGG 
CCCCAGGTGG CCCCACTGAC CCACAGGCCA GTAGGCCCAG CCCCCTCCAG GAGGGAGACT 
TTGGTGGAAG AGACCACAGC AGACTCCAGC GATGATGAGA TGGTGGAACC TTCCCAGTCT 
CTCCTCTCAG GTTATGTGAC CCCTGGGCTG ACTCCGGCCA GTTCCCAGGC TTCAAAGGCC 
ACTCCCAAGC CAGACCCCAA CCCCTCGGTT TCCTCTACTC CGGCCACCAG AGATGCCCCG 
GACGGCAAGC AGAAAGTGGA GCCCCAACAA GCAGCAGGCA CCATGTCCCC TAAAACAGGC 
AGAAGAGAGG CTAGCGCCAC ACCTCAGAAG CCCCAGAAGA CCGGGAAGGG GCCCAGGAGC 
CCCCAGGCCT CCGTGCTGGC GCTGCAGAGC GACATCGCCC GGCGCCTGCT GAGCGAGCCC 
TGGCCCCTGA GTGAGGCGCA GGTGCAGGCC TCGGTGGTGA AGGTGTTGGC GGAGCTGCTG 
GAGCAGGAGA AGAAGAAGGC CGTGGATGCC ACCAAGGAGG GCAGCCGGAA GGGCCGCCTG 
GGCAACAAGC GGAAGCTCTC CGAAGACCAG ACGGCCGCCG GTGCCCCCAG GAGCAAGAAG 
AAGAAGCGGC TGGCAGCCGG GGATGGCGGG GAGCGTGCAG TCTCCCCAGA AAAGGCCCCC 
AGGACTTCCA AGGGGAAATC CAAGAGGGAC AAAGCGAGTG GTGACATCAA GGAGAAGAAG 
GAGAAGGAGT CTCCCGGCTC CCAAGGGGCC AAGGAGAAGC CAGAAGGGGA GCCGGGGAAG 
GTGAAGGGTG AGGGGGGAGA CCAAGGCAAC CCAAAGAGCA AGAAGGAGAA GAAGAAATCC 
GACAAGAGTA AGTGCCCGGT GCAGCCCCGG AGCGCGTTCC CCAGCCCCGC CGAGCGCTGT 
CGGTCACCTC CCGGGCAGGC AGCCCTCAGC CAGCACAGAG ATGGGGCTGA GAGTGGCTTG 
TCCATCATGG GGGGGCAGGA TGGGCAAAGC CAGAACCAGG TGCCGGAGCA CAGGGGCCAC 
CACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TCOF1 cDNA ORF clone, Physeter catodon(sperm whale)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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