NOS1 cDNA ORF clone, Physeter catodon(sperm whale)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOS1
Entrez Gene ID	102991967
Full Name	nitric oxide synthase 1
Gene Type	protein-coding
Organism	Physeter catodon(sperm whale)

mRNA and Protein(s)

mRNA	Protein	Name
XM_028480071.1	XP_028335872.1	nitric oxide synthase, brain
XM_024120874.1	XP_023976642.1	nitric oxide synthase, brain
XM_007117287.1	XP_007117349.1	nitric oxide synthase, brain isoform X1

Pathways from BioSystems
Gene Ontology

The following NOS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPh130365	XM_007117287.1 Latest version!	Physeter catodon nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPh289487	XM_024120874.1 Latest version!	Physeter catodon nitric oxide synthase 1 (NOS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPh324864	XM_028480071.1 Latest version!	Physeter catodon nitric oxide synthase 1 (NOS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOS1 ( XM_007117287.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007117349.1 Physeter catodon nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPh130365
Clone ID Related Accession (Same CDS sequence)	XM_007117287.1
Accession Version	XM_007117287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006715462.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCA CCGAGCACAT CTGTATGGGG TCCATCATGC TTCCTTCTCA GCAAATACGA 
AGGCCTGAAG ACGTCCGCAC AAAAGAACAG CTCTTCCATC TTGCCAAAGA GTTTATTGAT 
CAGTACTATT CATCAATTAA AAGATTCGGC TCCAAAGCCC ATCTGGAGAG GCTGGAAGAG 
GTGAACAAAG AGATTGAAAC CACCAGCACC TACCAGCTCA AGGACACAGA GCTCATCTAC 
GGGGCCAAGC ACGCCTGGAG AAATGCCTCC CGCTGCGTCG GCCGGATCCA GTGGTCCAAG 
CTGCAGGTGT TCGATGCCCG CGACTGTACC ACGGCTCACG GGATGTTCAA CTACATCTGT 
AACCATATCA AGTATGCCAC CAACAAAGGG AACCTCAGAT CTGCCATCAC CATATTTCCC 
CAGAGGACCG ACGGCAAGCA TGACTTTCGA GTCTGGAACT CCCAGCTCAT CCGCTATGCT 
GGCTACAAGC AGCCTGACGG CTCCATCCTG GGGGATCCAG CCAGCGTGGA GTTCACACAG 
ATCTGCCTCC AGCAGGGCTG GAAACCCCCA AGAAGCCGCT TCGACATCCT GCCACTCCTG 
CTCCAGGCCA ACGGCAATGA CCCTGAGCTC TTCCAGATTC CTCCAGAGCT GGTGCTGGAA 
GTGCCCATCA GGCACCCCAA GTTTGAATGG TTCAAGGACC TTGGGCTGAA GTGGTACGGC 
CTCCCCGCTG TGTCAAACAT GCTCCTGGAG ATCGGTGGCC TGGAGTTCAG CGCCTGTCCC 
TTCAGCGGCT GGTACATGGG CACAGAGATT GGCGTCCGCG ACTACTGTGA CAGCTCCCGA 
TACAACATCC TGGAGGAAGT GGCCAAGAAG ATGAACTTGG ACATGAGGAA GACATCCTCC 
CTGTGGAAGG ACCAGGCACT GGTGGAGATC AACATTGCGG TGCTCTACAG CTTCCAGAGT 
GACAAAGTGA CCATCGTTGA CCACCACTCT GCCACCGAGT CCTTCATTAA GCACATGGAG 
AATGAATACC GCTGCCGGGG AGGCTGCCCC GCTGACTGGG TGTGGATTGT GCCCCCCATG 
TCTGGCAGCA TCACCCCCGT CTTCCACCAA GAGATGCTCA ACTACCGACT CACCCCCTCC 
TTCGAATACC AGCCTGACCC TTGGAACAGC CACGTCTGGA AAGGTACCAA CGGGACCCCC 
ACAAAGCGGC GAGCCATTGG CTTCAAGAAG CTGGCAGAAG CCGTCAAGTT CTCAGCCAAG 
CTGATGGGAC AGGCCATGGC CAAGAGGGTC AAGGCTACCA TCCTGTACGC CACAGAGACG 
GGCAAATCGC AGGCTTATGC CAAAACCCTG TGTGAGATCT TCAAACATGC CTTTGACGCC 
AAGGTGATGT CCATGGAAGA ATATGACATT GTACACCTGG AACATGAAAC TCTGGTCCTG 
GTGGTTACCA GCACCTTCGG AAACGGAGAC CCCCCTGAGA ATGGGGAGAA ATTTGGCTGC 
GCTTTGATGG AAATGAGGCA CCCCAACTCT GTGCACGAGG AAAGGAAGAG CTACAAGGTT 
CGTTTCAACA GTGTTTCCTC CTATTCCGAC TCCCGCAAAT CATCGGGCGA TGGGCCGGAC 
CTCAGAGACA ACTTTGAGAG CACGGGACCC CTGGCGAATG TGAGGTTCTC CGTGTTCGGC 
CTTGGCTCGC GGGCGTACCC TCACTTCTGC GCCTTTGGAC ACGCCGTGGA CACCCTCCTG 
GAAGAGCTGG GTGGGGAGAG GATCCTGAAG ATGAAGGAGG GGGATGAGCT CTGTGGGCAG 
GAAGAGGCTT TCAGGACCTG GGCCAAGAAG GTCTTCAAGG CAGCCTGTGA TGTGTTCTGC 
GTGGGAGATG ACGTCAACAT TGAGAAGGCA AACAACTCCC TCATCAGCAA TGACCGCAGT 
TGGAAGAGGA ACAAGTTCCG CCTCACCTAC ATGGCCGAAG CTCCAGAACT CACACAAGGT 
CTATCCAATG TCCACAAAAA GCGGGTCTCA GCCGCTCGAC TCCTCAGCCG TCAGAACCTT 
CAAAGTCCTA AATCCAGCCG ATCAACCATC TTCGTGCGTC TCCACACCAA CGGGAATCAG 
GAGCTGCAGT ACCAGCCCGG GGACCATCTG GGCGTCTTCC CCGGCAACCA CGAGGACCTC 
GTGAATGCCC TGATCGAGCG GCTGGAGGAT GCACCCCCGG TCAACCAGCT GGTGAAGGTG 
GAGCTGCTAG AGGAGCGAAA CACGGCTTTG GGGGTTATCA GTAACTGGAC CGATGAGCAT 
CGCCTCCCAC CCTGCACCAT CTTCCAGGCC TTCAAGTACT ACCTGGATAT CACCACGCCT 
CCAACGCCAC TGCAGCTGCA GCAGTTTGCC TCTCTGGCCA CCAGTGAGAA AGAGAAGCAG 
CGTCTGCTGG TCCTCAGCAA GGGTTTGCAG GAGTACGAGG AATGGAAATG GGGCAGGAAC 
CCGAACATCG TGGAGGTGCT GGAGGAGTTC CCATCCATCC AGATGCCCGC CACCCTGCTC 
CTGACCCAGC TGTCGCTGCT GCAACCTCGC TACTATTCCA TCAGCTCCTC CCCGGACATG 
TACCCCCACG AGGTGCACCT CACCGTGGCC GTCGTCTCCT ACCGCACCCG AGATGGAGAA 
GGACCGATTC ACCACGGTGT GTGCTCCTCC TGGCTCAACC GGATCCAGGC TGACGAACTG 
GTGCCCTGTT TCGTGAGAGG AGCGCCCAGC TTCCACCTGC CCCGAAATCC CCAAGTGCCC 
TGTATCCTGG TTGGCCCGGG CACGGGCATT GCCCCTTTCC GAAGCTTCTG GCAGCAACGG 
CAATTTGATA TCCAACACAA AGGAATGAGT CCCTGCCCCA TGGTCCTGGT CTTCGGGTGC 
CGGCAGTCCA AGATCGACCA CATCTACAGG GAGGAGACCC TACAGGCCAA GAGCAAGGGG 
GTCTTCAGAG AACTGTACAC AGCCTACTCC CGGGAGCCAG ACAAACCAAA GAAGTACGTG 
CAGGACATCC TACAGGAGCA GCTGGCAGAA CCCGTGTACC GAGCCCTGAA GGAGCAAGGG 
GGCCACATTT ACGTCTGCGG GGACGTCACC ATGGCAGCCG ATGTCCTCAA AGCCATCCAG 
CGCATCATGA CCCAGCAGGG GAAGCTCTCG GTGGAGGATG CCGGCGTGTT CATCAGCAGG 
CTGAGGGATG ACAACCGGTA CCATGAGGAT ATTTTCGGGG TCACCCTGCG CACATATGAA 
GTGACCAACC GCCTTAGATC TGAATCCATT GCCTTCATTG AGGAGAGCAA AAAGGACACA 
GATGAGGTTT TCAGCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007117349.1
CDS	58..3378
Translation	MGCTEHICMGSIMLPSQQIRRPEDVRTKEQLFHLAKEFIDQYYSSIKRFGSKAHLERLEEVNKEIETTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPASVEFTQICLQQGWKPPRSRFDILPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDSSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNSHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVHEERKSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMKEGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYMAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQLVKVELLEERNTALGVISNWTDEHRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGRNPNIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPHEVHLTVAVVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMSPCPMVLVFGCRQSKIDHIYREETLQAKSKGVFRELYTAYSREPDKPKKYVQDILQEQLAEPVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSVEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_007117287.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007117287.1

ORF Insert Sequence:

ATGGGATGCA CCGAGCACAT CTGTATGGGG TCCATCATGC TTCCTTCTCA GCAAATACGA 
AGGCCTGAAG ACGTCCGCAC AAAAGAACAG CTCTTCCATC TTGCCAAAGA GTTTATTGAT 
CAGTACTATT CATCAATTAA AAGATTCGGC TCCAAAGCCC ATCTGGAGAG GCTGGAAGAG 
GTGAACAAAG AGATTGAAAC CACCAGCACC TACCAGCTCA AGGACACAGA GCTCATCTAC 
GGGGCCAAGC ACGCCTGGAG AAATGCCTCC CGCTGCGTCG GCCGGATCCA GTGGTCCAAG 
CTGCAGGTGT TCGATGCCCG CGACTGTACC ACGGCTCACG GGATGTTCAA CTACATCTGT 
AACCATATCA AGTATGCCAC CAACAAAGGG AACCTCAGAT CTGCCATCAC CATATTTCCC 
CAGAGGACCG ACGGCAAGCA TGACTTTCGA GTCTGGAACT CCCAGCTCAT CCGCTATGCT 
GGCTACAAGC AGCCTGACGG CTCCATCCTG GGGGATCCAG CCAGCGTGGA GTTCACACAG 
ATCTGCCTCC AGCAGGGCTG GAAACCCCCA AGAAGCCGCT TCGACATCCT GCCACTCCTG 
CTCCAGGCCA ACGGCAATGA CCCTGAGCTC TTCCAGATTC CTCCAGAGCT GGTGCTGGAA 
GTGCCCATCA GGCACCCCAA GTTTGAATGG TTCAAGGACC TTGGGCTGAA GTGGTACGGC 
CTCCCCGCTG TGTCAAACAT GCTCCTGGAG ATCGGTGGCC TGGAGTTCAG CGCCTGTCCC 
TTCAGCGGCT GGTACATGGG CACAGAGATT GGCGTCCGCG ACTACTGTGA CAGCTCCCGA 
TACAACATCC TGGAGGAAGT GGCCAAGAAG ATGAACTTGG ACATGAGGAA GACATCCTCC 
CTGTGGAAGG ACCAGGCACT GGTGGAGATC AACATTGCGG TGCTCTACAG CTTCCAGAGT 
GACAAAGTGA CCATCGTTGA CCACCACTCT GCCACCGAGT CCTTCATTAA GCACATGGAG 
AATGAATACC GCTGCCGGGG AGGCTGCCCC GCTGACTGGG TGTGGATTGT GCCCCCCATG 
TCTGGCAGCA TCACCCCCGT CTTCCACCAA GAGATGCTCA ACTACCGACT CACCCCCTCC 
TTCGAATACC AGCCTGACCC TTGGAACAGC CACGTCTGGA AAGGTACCAA CGGGACCCCC 
ACAAAGCGGC GAGCCATTGG CTTCAAGAAG CTGGCAGAAG CCGTCAAGTT CTCAGCCAAG 
CTGATGGGAC AGGCCATGGC CAAGAGGGTC AAGGCTACCA TCCTGTACGC CACAGAGACG 
GGCAAATCGC AGGCTTATGC CAAAACCCTG TGTGAGATCT TCAAACATGC CTTTGACGCC 
AAGGTGATGT CCATGGAAGA ATATGACATT GTACACCTGG AACATGAAAC TCTGGTCCTG 
GTGGTTACCA GCACCTTCGG AAACGGAGAC CCCCCTGAGA ATGGGGAGAA ATTTGGCTGC 
GCTTTGATGG AAATGAGGCA CCCCAACTCT GTGCACGAGG AAAGGAAGAG CTACAAGGTT 
CGTTTCAACA GTGTTTCCTC CTATTCCGAC TCCCGCAAAT CATCGGGCGA TGGGCCGGAC 
CTCAGAGACA ACTTTGAGAG CACGGGACCC CTGGCGAATG TGAGGTTCTC CGTGTTCGGC 
CTTGGCTCGC GGGCGTACCC TCACTTCTGC GCCTTTGGAC ACGCCGTGGA CACCCTCCTG 
GAAGAGCTGG GTGGGGAGAG GATCCTGAAG ATGAAGGAGG GGGATGAGCT CTGTGGGCAG 
GAAGAGGCTT TCAGGACCTG GGCCAAGAAG GTCTTCAAGG CAGCCTGTGA TGTGTTCTGC 
GTGGGAGATG ACGTCAACAT TGAGAAGGCA AACAACTCCC TCATCAGCAA TGACCGCAGT 
TGGAAGAGGA ACAAGTTCCG CCTCACCTAC ATGGCCGAAG CTCCAGAACT CACACAAGGT 
CTATCCAATG TCCACAAAAA GCGGGTCTCA GCCGCTCGAC TCCTCAGCCG TCAGAACCTT 
CAAAGTCCTA AATCCAGCCG ATCAACCATC TTCGTGCGTC TCCACACCAA CGGGAATCAG 
GAGCTGCAGT ACCAGCCCGG GGACCATCTG GGCGTCTTCC CCGGCAACCA CGAGGACCTC 
GTGAATGCCC TGATCGAGCG GCTGGAGGAT GCACCCCCGG TCAACCAGCT GGTGAAGGTG 
GAGCTGCTAG AGGAGCGAAA CACGGCTTTG GGGGTTATCA GTAACTGGAC CGATGAGCAT 
CGCCTCCCAC CCTGCACCAT CTTCCAGGCC TTCAAGTACT ACCTGGATAT CACCACGCCT 
CCAACGCCAC TGCAGCTGCA GCAGTTTGCC TCTCTGGCCA CCAGTGAGAA AGAGAAGCAG 
CGTCTGCTGG TCCTCAGCAA GGGTTTGCAG GAGTACGAGG AATGGAAATG GGGCAGGAAC 
CCGAACATCG TGGAGGTGCT GGAGGAGTTC CCATCCATCC AGATGCCCGC CACCCTGCTC 
CTGACCCAGC TGTCGCTGCT GCAACCTCGC TACTATTCCA TCAGCTCCTC CCCGGACATG 
TACCCCCACG AGGTGCACCT CACCGTGGCC GTCGTCTCCT ACCGCACCCG AGATGGAGAA 
GGACCGATTC ACCACGGTGT GTGCTCCTCC TGGCTCAACC GGATCCAGGC TGACGAACTG 
GTGCCCTGTT TCGTGAGAGG AGCGCCCAGC TTCCACCTGC CCCGAAATCC CCAAGTGCCC 
TGTATCCTGG TTGGCCCGGG CACGGGCATT GCCCCTTTCC GAAGCTTCTG GCAGCAACGG 
CAATTTGATA TCCAACACAA AGGAATGAGT CCCTGCCCCA TGGTCCTGGT CTTCGGGTGC 
CGGCAGTCCA AGATCGACCA CATCTACAGG GAGGAGACCC TACAGGCCAA GAGCAAGGGG 
GTCTTCAGAG AACTGTACAC AGCCTACTCC CGGGAGCCAG ACAAACCAAA GAAGTACGTG 
CAGGACATCC TACAGGAGCA GCTGGCAGAA CCCGTGTACC GAGCCCTGAA GGAGCAAGGG 
GGCCACATTT ACGTCTGCGG GGACGTCACC ATGGCAGCCG ATGTCCTCAA AGCCATCCAG 
CGCATCATGA CCCAGCAGGG GAAGCTCTCG GTGGAGGATG CCGGCGTGTT CATCAGCAGG 
CTGAGGGATG ACAACCGGTA CCATGAGGAT ATTTTCGGGG TCACCCTGCG CACATATGAA 
GTGACCAACC GCCTTAGATC TGAATCCATT GCCTTCATTG AGGAGAGCAA AAAGGACACA 
GATGAGGTTT TCAGCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_024120874.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_023976642.1 Physeter catodon nitric oxide synthase 1 (NOS1), mRNA.

Price & Availability↑

CloneID	OPh289487
Clone ID Related Accession (Same CDS sequence)	XM_024120874.1
Accession Version	XM_024120874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	nitric oxide synthase, brain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATC ACACGTTCAG CGTCAAGCAG ATCCAACCCA ATGTCATTTC TGTCCGCCTC 
TTCAAGCGCA AAGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGAGGGTGAG CAAGCCACCT 
GTGATCATCT CCGACCTGAT TCGAGGGGGC GCCGCGGAGC AGAGCGGCCT CATCCAGGCT 
GGGGACATCA TTCTCGCCGT CAACGGCCAG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCC 
GAGGGCTTCA CCACGCACCT GGAGACCACT TTCACTGGGG ACGGGACCCC CAAGACCATC 
CGGGTGACAC GGCCCCTGGG TCCGCCAACC TCAGCCGTCG ATCTGTCCCA CCAGCCATCA 
GCCAGCAAAG AGCAGACCCT GGCAGTGGAC GGGGCCACGG GTCCTGGCAA TGGGCCGCAG 
CACGTGCCAG ACCAGGGGCA GGAGGCTGGC TTGCTCTCCC ACACCAATGG CCTGGCTCCC 
AGGCCCCCGA GCCAGGACCC TGCCAAGAAG AGTTCTGGGG AGAACAGTCA ACTGCTCAGA 
GAGATAGAGC CTGTGCTGAA TCTTCTCACC AGCGGGAGCA AAGGGATCAG CGGAGGGGGA 
CCTGCCAGAG CGGAGACAAA GGACGCAGAA ATCCAGGTGG ACAGAGATCT GGACGGCAAA 
TCACACAGAC CGCTGCCCCT CGGTGTGGAG AACGACCGCG TCTTCAATGA CCTATGGGGG 
AAGGGCAACA TGCCAGTCGT CCTCAACAAC CCGTATTCAG AGAAGGAGCA GCCCCCTGCC 
TCGGGAAAAC AGTCTCCCAT AAAGAATGGC AGCCCCTCCA AGTGCCCTCG CTTCCTCAAG 
GTCAAGAACT GGGAGACCGA TGTGGTTCTC TCTGACACCC TCCACCTTAA GAGCACCTTG 
GGAATGGGAT GCACCGAGCA CATCTGTATG GGGTCCATCA TGCTTCCTTC TCAGCAAATA 
CGAAGGCCTG AAGACGTCCG CACAAAAGAA CAGCTCTTCC ATCTTGCCAA AGAGTTTATT 
GATCAGTACT ATTCATCAAT TAAAAGATTC GGCTCCAAAG CCCATCTGGA GAGGCTGGAA 
GAGGTGAACA AAGAGATTGA AACCACCAGC ACCTACCAGC TCAAGGACAC AGAGCTCATC 
TACGGGGCCA AGCACGCCTG GAGAAATGCC TCCCGCTGCG TCGGCCGGAT CCAGTGGTCC 
AAGCTGCAGG TGTTCGATGC CCGCGACTGT ACCACGGCTC ACGGGATGTT CAACTACATC 
TGTAACCATA TCAAGTATGC CACCAACAAA GGGAACCTCA GATCTGCCAT CACCATATTT 
CCCCAGAGGA CCGACGGCAA GCATGACTTT CGAGTCTGGA ACTCCCAGCT CATCCGCTAT 
GCTGGCTACA AGCAGCCTGA CGGCTCCATC CTGGGGGATC CAGCCAGCGT GGAGTTCACA 
CAGATCTGCC TCCAGCAGGG CTGGAAACCC CCAAGAAGCC GCTTCGACAT CCTGCCACTC 
CTGCTCCAGG CCAACGGCAA TGACCCTGAG CTCTTCCAGA TTCCTCCAGA GCTGGTGCTG 
GAAGTGCCCA TCAGGCACCC CAAGTTTGAA TGGTTCAAGG ACCTTGGGCT GAAGTGGTAC 
GGCCTCCCCG CTGTGTCAAA CATGCTCCTG GAGATCGGTG GCCTGGAGTT CAGCGCCTGT 
CCCTTCAGCG GCTGGTACAT GGGCACAGAG ATTGGCGTCC GCGACTACTG TGACAGCTCC 
CGATACAACA TCCTGGAGGA AGTGGCCAAG AAGATGAACT TGGACATGAG GAAGACATCC 
TCCCTGTGGA AGGACCAGGC ACTGGTGGAG ATCAACATTG CGGTGCTCTA CAGCTTCCAG 
AGTGACAAAG TGACCATCGT TGACCACCAC TCTGCCACCG AGTCCTTCAT TAAGCACATG 
GAGAATGAAT ACCGCTGCCG GGGAGGCTGC CCCGCTGACT GGGTGTGGAT TGTGCCCCCC 
ATGTCTGGCA GCATCACCCC CGTCTTCCAC CAAGAGATGC TCAACTACCG ACTCACCCCC 
TCCTTCGAAT ACCAGCCTGA CCCTTGGAAC AGCCACGTCT GGAAAGGTAC CAACGGGACC 
CCCACAAAGC GGCGAGCCAT TGGCTTCAAG AAGCTGGCAG AAGCCGTCAA GTTCTCAGCC 
AAGCTAATGG GACAGGCCAT GGCCAAGAGG GTCAAGGCTA CCATCCTGTA CGCCACAGAG 
ACGGGCAAAT CGCAGGCTTA TGCCAAAACC CTGTGTGAGA TCTTCAAACA TGCCTTTGAC 
GCCAAGGTGA TGTCCATGGA AGAATATGAC ATTGTACACC TGGAACATGA AACTCTGGTC 
CTGGTGGTTA CCAGCACCTT CGGAAACGGA GACCCCCCTG AGAATGGGGA GAAATTTGGC 
TGCGCTTTGA TGGAAATGAG GCACCCCAAC TCTGTGCACG AGGAAAGGAA GAGCTACAAG 
GTTCGTTTCA ACAGTGTTTC CTCCTATTCC GACTCCCGCA AATCATCGGG CAATGGGCCG 
GACCTCAGAG ACAACTTTGA GAGCACGGGA CCCCTGGCGA ATGTGAGGTT CTCCGTGTTC 
GGCCTTGGCT CGCGGGCGTA CCCTCACTTC TGCGCCTTTG GACACGCCGT GGACACCCTC 
CTGGAAGAGC TGGGTGGGGA GAGGATCCTG AAGATGAAGG AGGGGGATGA GCTCTGTGGG 
CAGGAAGAGG CTTTCAGGAC CTGGGCCAAG AAGGTCTTCA AGGCAGCCTG TGATGTGTTC 
TGCGTGGGAG ATGACGTCAA CATTGAGAAG GCAAACAACT CCCTCATCAG CAATGACCGC 
AGTTGGAAGA GGAACAAGTT CCGCCTCACC TACATGGCCG AAGCTCCAGA ACTCACACAA 
GGTCTATCCA ATGTCCACAA AAAGCGGGTC TCAGCCGCTC GACTCCTCAG CCGTCAGAAC 
CTTCAAAGTC CTAAATCCAG CCGATCAACC ATCTTCGTGC GTCTCCACAC CAACGGGAAT 
CAGGAGCTGC AGTACCAGCC CGGGGACCAT CTGGGCGTCT TCCCCGGCAA CCACGAGGAC 
CTCGTGAATG CCCTGATCGA GCGGCTGGAG GATGCACCCC CGGTCAACCA GCTGGTGAAG 
GTGGAGCTGC TAGAGGAGCG AAACACGGCT TTGGGGGTTA TCAGTAACTG GACCGATGAG 
CATCGCCTCC CACCCTGCAC CATCTTCCAG GCCTTCAAGT ACTACCTGGA TATCACCACG 
CCTCCAACGC CACTGCAGCT GCAGCAGTTT GCCTCTCTGG CCACCAGTGA GAAAGAGAAG 
CAGCGTCTGC TGGTCCTCAG CAAGGGTTTG CAGGAGTACG AGGAATGGAA ATGGGGCAGG 
AACCCGAACA TCGTGGAGGT GCTGGAGGAG TTCCCATCCA TCCAGATGCC CGCCACCCTG 
CTCCTGACCC AGCTGTCGCT GCTGCAACCT CGCTACTATT CCATCAGCTC CTCCCCGGAC 
ATGTACCCCC ACGAGGTGCA CCTCACCGTG GCCGTCGTCT CCTACCGCAC CCGAGATGGA 
GAAGGACCGA TTCACCACGG TGTGTGCTCC TCCTGGCTCA ACCGGATCCA GGCTGACGAA 
CTGGTGCCCT GTTTCGTGAG AGGAGCGCCC AGCTTCCACC TGCCCCGAAA TCCCCAAGTG 
CCCTGTATCC TGGTTGGCCC GGGCACGGGC ATTGCCCCTT TCCGAAGCTT CTGGCAGCAA 
CGGCAATTTG ATATCCAACA CAAAGGAATG AGTCCCTGCC CCATGGTCCT GGTCTTCGGG 
TGCCGGCAGT CCAAGATCGA CCACATCTAC AGGGAGGAGA CCCTACAGGC CAAGAGCAAG 
GGGGTCTTCA GAGAACTGTA CACAGCCTAC TCCCGGGAGC CAGACAAACC AAAGAAGTAC 
GTGCAGGACA TCCTACAGGA GCAGCTGGCA GAACCCGTGT ACCGAGCCCT GAAGGAGCAA 
GGGGGCCACA TTTACGTCTG CGGGGACGTC ACCATGGCAG CCGATGTCCT CAAAGCCATC 
CAGCGCATCA TGACCCAGCA GGGGAAGCTC TCGGTGGAGG ATGCCGGCGT GTTCATCAGC 
AGGCTGAGGG ATGACAACCG GTACCATGAG GATATTTTCG GGGTCACCCT GCGCACATAT 
GAAGTGACCA ACCGCCTTAG ATCTGAATCC ATTGCCTTCA TTGAGGAGAG CAAAAAGGAC 
ACAGATGAGG TTTTCAGCTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023976642.1
CDS	1..4284
Translation	MENHTFSVKQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGQPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTRPLGPPTSAVDLSHQPSASKEQTLAVDGATGPGNGPQHVPDQGQEAGLLSHTNGLAPRPPSQDPAKKSSGENSQLLREIEPVLNLLTSGSKGISGGGPARAETKDAEIQVDRDLDGKSHRPLPLGVENDRVFNDLWGKGNMPVVLNNPYSEKEQPPASGKQSPIKNGSPSKCPRFLKVKNWETDVVLSDTLHLKSTLGMGCTEHICMGSIMLPSQQIRRPEDVRTKEQLFHLAKEFIDQYYSSIKRFGSKAHLERLEEVNKEIETTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPASVEFTQICLQQGWKPPRSRFDILPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDSSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNSHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVHEERKSYKVRFNSVSSYSDSRKSSGNGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMKEGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYMAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQLVKVELLEERNTALGVISNWTDEHRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGRNPNIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPHEVHLTVAVVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMSPCPMVLVFGCRQSKIDHIYREETLQAKSKGVFRELYTAYSREPDKPKKYVQDILQEQLAEPVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSVEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_024120874.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon nitric oxide synthase 1 (NOS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024120874.1

ORF Insert Sequence:

ATGGAGAATC ACACGTTCAG CGTCAAGCAG ATCCAACCCA ATGTCATTTC TGTCCGCCTC 
TTCAAGCGCA AAGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGAGGGTGAG CAAGCCACCT 
GTGATCATCT CCGACCTGAT TCGAGGGGGC GCCGCGGAGC AGAGCGGCCT CATCCAGGCT 
GGGGACATCA TTCTCGCCGT CAACGGCCAG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCC 
GAGGGCTTCA CCACGCACCT GGAGACCACT TTCACTGGGG ACGGGACCCC CAAGACCATC 
CGGGTGACAC GGCCCCTGGG TCCGCCAACC TCAGCCGTCG ATCTGTCCCA CCAGCCATCA 
GCCAGCAAAG AGCAGACCCT GGCAGTGGAC GGGGCCACGG GTCCTGGCAA TGGGCCGCAG 
CACGTGCCAG ACCAGGGGCA GGAGGCTGGC TTGCTCTCCC ACACCAATGG CCTGGCTCCC 
AGGCCCCCGA GCCAGGACCC TGCCAAGAAG AGTTCTGGGG AGAACAGTCA ACTGCTCAGA 
GAGATAGAGC CTGTGCTGAA TCTTCTCACC AGCGGGAGCA AAGGGATCAG CGGAGGGGGA 
CCTGCCAGAG CGGAGACAAA GGACGCAGAA ATCCAGGTGG ACAGAGATCT GGACGGCAAA 
TCACACAGAC CGCTGCCCCT CGGTGTGGAG AACGACCGCG TCTTCAATGA CCTATGGGGG 
AAGGGCAACA TGCCAGTCGT CCTCAACAAC CCGTATTCAG AGAAGGAGCA GCCCCCTGCC 
TCGGGAAAAC AGTCTCCCAT AAAGAATGGC AGCCCCTCCA AGTGCCCTCG CTTCCTCAAG 
GTCAAGAACT GGGAGACCGA TGTGGTTCTC TCTGACACCC TCCACCTTAA GAGCACCTTG 
GGAATGGGAT GCACCGAGCA CATCTGTATG GGGTCCATCA TGCTTCCTTC TCAGCAAATA 
CGAAGGCCTG AAGACGTCCG CACAAAAGAA CAGCTCTTCC ATCTTGCCAA AGAGTTTATT 
GATCAGTACT ATTCATCAAT TAAAAGATTC GGCTCCAAAG CCCATCTGGA GAGGCTGGAA 
GAGGTGAACA AAGAGATTGA AACCACCAGC ACCTACCAGC TCAAGGACAC AGAGCTCATC 
TACGGGGCCA AGCACGCCTG GAGAAATGCC TCCCGCTGCG TCGGCCGGAT CCAGTGGTCC 
AAGCTGCAGG TGTTCGATGC CCGCGACTGT ACCACGGCTC ACGGGATGTT CAACTACATC 
TGTAACCATA TCAAGTATGC CACCAACAAA GGGAACCTCA GATCTGCCAT CACCATATTT 
CCCCAGAGGA CCGACGGCAA GCATGACTTT CGAGTCTGGA ACTCCCAGCT CATCCGCTAT 
GCTGGCTACA AGCAGCCTGA CGGCTCCATC CTGGGGGATC CAGCCAGCGT GGAGTTCACA 
CAGATCTGCC TCCAGCAGGG CTGGAAACCC CCAAGAAGCC GCTTCGACAT CCTGCCACTC 
CTGCTCCAGG CCAACGGCAA TGACCCTGAG CTCTTCCAGA TTCCTCCAGA GCTGGTGCTG 
GAAGTGCCCA TCAGGCACCC CAAGTTTGAA TGGTTCAAGG ACCTTGGGCT GAAGTGGTAC 
GGCCTCCCCG CTGTGTCAAA CATGCTCCTG GAGATCGGTG GCCTGGAGTT CAGCGCCTGT 
CCCTTCAGCG GCTGGTACAT GGGCACAGAG ATTGGCGTCC GCGACTACTG TGACAGCTCC 
CGATACAACA TCCTGGAGGA AGTGGCCAAG AAGATGAACT TGGACATGAG GAAGACATCC 
TCCCTGTGGA AGGACCAGGC ACTGGTGGAG ATCAACATTG CGGTGCTCTA CAGCTTCCAG 
AGTGACAAAG TGACCATCGT TGACCACCAC TCTGCCACCG AGTCCTTCAT TAAGCACATG 
GAGAATGAAT ACCGCTGCCG GGGAGGCTGC CCCGCTGACT GGGTGTGGAT TGTGCCCCCC 
ATGTCTGGCA GCATCACCCC CGTCTTCCAC CAAGAGATGC TCAACTACCG ACTCACCCCC 
TCCTTCGAAT ACCAGCCTGA CCCTTGGAAC AGCCACGTCT GGAAAGGTAC CAACGGGACC 
CCCACAAAGC GGCGAGCCAT TGGCTTCAAG AAGCTGGCAG AAGCCGTCAA GTTCTCAGCC 
AAGCTAATGG GACAGGCCAT GGCCAAGAGG GTCAAGGCTA CCATCCTGTA CGCCACAGAG 
ACGGGCAAAT CGCAGGCTTA TGCCAAAACC CTGTGTGAGA TCTTCAAACA TGCCTTTGAC 
GCCAAGGTGA TGTCCATGGA AGAATATGAC ATTGTACACC TGGAACATGA AACTCTGGTC 
CTGGTGGTTA CCAGCACCTT CGGAAACGGA GACCCCCCTG AGAATGGGGA GAAATTTGGC 
TGCGCTTTGA TGGAAATGAG GCACCCCAAC TCTGTGCACG AGGAAAGGAA GAGCTACAAG 
GTTCGTTTCA ACAGTGTTTC CTCCTATTCC GACTCCCGCA AATCATCGGG CAATGGGCCG 
GACCTCAGAG ACAACTTTGA GAGCACGGGA CCCCTGGCGA ATGTGAGGTT CTCCGTGTTC 
GGCCTTGGCT CGCGGGCGTA CCCTCACTTC TGCGCCTTTG GACACGCCGT GGACACCCTC 
CTGGAAGAGC TGGGTGGGGA GAGGATCCTG AAGATGAAGG AGGGGGATGA GCTCTGTGGG 
CAGGAAGAGG CTTTCAGGAC CTGGGCCAAG AAGGTCTTCA AGGCAGCCTG TGATGTGTTC 
TGCGTGGGAG ATGACGTCAA CATTGAGAAG GCAAACAACT CCCTCATCAG CAATGACCGC 
AGTTGGAAGA GGAACAAGTT CCGCCTCACC TACATGGCCG AAGCTCCAGA ACTCACACAA 
GGTCTATCCA ATGTCCACAA AAAGCGGGTC TCAGCCGCTC GACTCCTCAG CCGTCAGAAC 
CTTCAAAGTC CTAAATCCAG CCGATCAACC ATCTTCGTGC GTCTCCACAC CAACGGGAAT 
CAGGAGCTGC AGTACCAGCC CGGGGACCAT CTGGGCGTCT TCCCCGGCAA CCACGAGGAC 
CTCGTGAATG CCCTGATCGA GCGGCTGGAG GATGCACCCC CGGTCAACCA GCTGGTGAAG 
GTGGAGCTGC TAGAGGAGCG AAACACGGCT TTGGGGGTTA TCAGTAACTG GACCGATGAG 
CATCGCCTCC CACCCTGCAC CATCTTCCAG GCCTTCAAGT ACTACCTGGA TATCACCACG 
CCTCCAACGC CACTGCAGCT GCAGCAGTTT GCCTCTCTGG CCACCAGTGA GAAAGAGAAG 
CAGCGTCTGC TGGTCCTCAG CAAGGGTTTG CAGGAGTACG AGGAATGGAA ATGGGGCAGG 
AACCCGAACA TCGTGGAGGT GCTGGAGGAG TTCCCATCCA TCCAGATGCC CGCCACCCTG 
CTCCTGACCC AGCTGTCGCT GCTGCAACCT CGCTACTATT CCATCAGCTC CTCCCCGGAC 
ATGTACCCCC ACGAGGTGCA CCTCACCGTG GCCGTCGTCT CCTACCGCAC CCGAGATGGA 
GAAGGACCGA TTCACCACGG TGTGTGCTCC TCCTGGCTCA ACCGGATCCA GGCTGACGAA 
CTGGTGCCCT GTTTCGTGAG AGGAGCGCCC AGCTTCCACC TGCCCCGAAA TCCCCAAGTG 
CCCTGTATCC TGGTTGGCCC GGGCACGGGC ATTGCCCCTT TCCGAAGCTT CTGGCAGCAA 
CGGCAATTTG ATATCCAACA CAAAGGAATG AGTCCCTGCC CCATGGTCCT GGTCTTCGGG 
TGCCGGCAGT CCAAGATCGA CCACATCTAC AGGGAGGAGA CCCTACAGGC CAAGAGCAAG 
GGGGTCTTCA GAGAACTGTA CACAGCCTAC TCCCGGGAGC CAGACAAACC AAAGAAGTAC 
GTGCAGGACA TCCTACAGGA GCAGCTGGCA GAACCCGTGT ACCGAGCCCT GAAGGAGCAA 
GGGGGCCACA TTTACGTCTG CGGGGACGTC ACCATGGCAG CCGATGTCCT CAAAGCCATC 
CAGCGCATCA TGACCCAGCA GGGGAAGCTC TCGGTGGAGG ATGCCGGCGT GTTCATCAGC 
AGGCTGAGGG ATGACAACCG GTACCATGAG GATATTTTCG GGGTCACCCT GCGCACATAT 
GAAGTGACCA ACCGCCTTAG ATCTGAATCC ATTGCCTTCA TTGAGGAGAG CAAAAAGGAC 
ACAGATGAGG TTTTCAGCTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_028480071.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_028335872.1 Physeter catodon nitric oxide synthase 1 (NOS1), mRNA.

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CloneID	OPh324864
Clone ID Related Accession (Same CDS sequence)	XM_028480071.1
Accession Version	XM_028480071.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	nitric oxide synthase, brain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041232.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATC ACACGTTCAG CGTCAAGCAG ATCCAACCCA ATGTCATTTC TGTCCGCCTC 
TTCAAGCGCA AAGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGAGGGTGAG CAAGCCACCT 
GTGATCATCT CCGACCTGAT TCGAGGGGGC GCCGCGGAGC AGAGCGGCCT CATCCAGGCT 
GGGGACATCA TTCTCGCCGT CAACGGCCAG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCC 
GAGGGCTTCA CCACGCACCT GGAGACCACT TTCACTGGGG ACGGGACCCC CAAGACCATC 
CGGGTGACAC GGCCCCTGGG TCCGCCAACC TCAGCCGTCG ATCTGTCCCA CCAGCCATCA 
GCCAGCAAAG AGCAGACCCT GGCAGTGGAC GGGGCCACGG GTCCTGGCAA TGGGCCGCAG 
CACGTGCCAG ACCAGGGGCA GGAGGCTGGC TTGCTCTCCC ACACCAATGG CCTGGCTCCC 
AGGCCCCCGA GCCAGGACCC TGCCAAGAAG AGTTCTGGGG AGAACAGTCA ACTGCTCAGA 
GAGATAGAGC CTGTGCTGAA TCTTCTCACC AGCGGGAGCA AAGGGATCAG CGGAGGGGGA 
CCTGCCAGAG CGGAGACAAA GGACGCAGAA ATCCAGGTGG ACAGAGATCT GGACGGCAAA 
TCACACAGAC CGCTGCCCCT CGGTGTGGAG AACGACCGCG TCTTCAATGA CCTATGGGGG 
AAGGGCAACA TGCCAGTCGT CCTCAACAAC CCGTATTCAG AGAAGGAGCA GCCCCCTGCC 
TCGGGAAAAC AGTCTCCCAT AAAGAATGGC AGCCCCTCCA AGTGCCCTCG CTTCCTCAAG 
GTCAAGAACT GGGAGACCGA TGTGGTTCTC TCTGACACCC TCCACCTTAA GAGCACCTTG 
GGAATGGGAT GCACCGAGCA CATCTGTATG GGGTCCATCA TGCTTCCTTC TCAGCAAATA 
CGAAGGCCTG AAGACGTCCG CACAAAAGAA CAGCTCTTCC ATCTTGCCAA AGAGTTTATT 
GATCAGTACT ATTCATCAAT TAAAAGATTC GGCTCCAAAG CCCATCTGGA GAGGCTGGAA 
GAGGTGAACA AAGAGATTGA AACCACCAGC ACCTACCAGC TCAAGGACAC AGAGCTCATC 
TACGGGGCCA AGCACGCCTG GAGAAATGCC TCCCGCTGCG TCGGCCGGAT CCAGTGGTCC 
AAGCTGCAGG TGTTCGATGC CCGCGACTGT ACCACGGCTC ACGGGATGTT CAACTACATC 
TGTAACCATA TCAAGTATGC CACCAACAAA GGGAACCTCA GATCTGCCAT CACCATATTT 
CCCCAGAGGA CCGACGGCAA GCATGACTTT CGAGTCTGGA ACTCCCAGCT CATCCGCTAT 
GCTGGCTACA AGCAGCCTGA CGGCTCCATC CTGGGGGATC CAGCCAGCGT GGAGTTCACA 
CAGATCTGCC TCCAGCAGGG CTGGAAACCC CCAAGAAGCC GCTTCGACAT CCTGCCACTC 
CTGCTCCAGG CCAACGGCAA TGACCCTGAG CTCTTCCAGA TTCCTCCAGA GCTGGTGCTG 
GAAGTGCCCA TCAGGCACCC CAAGTTTGAA TGGTTCAAGG ACCTTGGGCT GAAGTGGTAC 
GGCCTCCCCG CTGTGTCAAA CATGCTCCTG GAGATCGGTG GCCTGGAGTT CAGCGCCTGT 
CCCTTCAGCG GCTGGTACAT GGGCACAGAG ATTGGCGTCC GCGACTACTG TGACAGCTCC 
CGATACAACA TCCTGGAGGA AGTGGCCAAG AAGATGAACT TGGACATGAG GAAGACATCC 
TCCCTGTGGA AGGACCAGGC ACTGGTGGAG ATCAACATTG CGGTGCTCTA CAGCTTCCAG 
AGTGACAAAG TGACCATCGT TGACCACCAC TCTGCCACCG AGTCCTTCAT TAAGCACATG 
GAGAATGAAT ACCGCTGCCG GGGAGGCTGC CCCGCTGACT GGGTGTGGAT TGTGCCCCCC 
ATGTCTGGCA GCATCACCCC CGTCTTCCAC CAAGAGATGC TCAACTACCG ACTCACCCCC 
TCCTTCGAAT ACCAGCCTGA CCCTTGGAAC AGCCACGTCT GGAAAGGTAC CAACGGGACC 
CCCACAAAGC GGCGAGCCAT TGGCTTCAAG AAGCTGGCAG AAGCCGTCAA GTTCTCAGCC 
AAGCTAATGG GACAGGCCAT GGCCAAGAGG GTCAAGGCTA CCATCCTGTA CGCCACAGAG 
ACGGGCAAAT CGCAGGCTTA TGCCAAAACC CTGTGTGAGA TCTTCAAACA TGCCTTTGAC 
GCCAAGGTGA TGTCCATGGA AGAATATGAC ATTGTACACC TGGAACATGA AACTCTGGTC 
CTGGTGGTTA CCAGCACCTT CGGAAACGGA GACCCCCCTG AGAATGGGGA GAAATTTGGC 
TGCGCTTTGA TGGAAATGAG GCACCCCAAC TCTGTGCACG AGGAAAGGAA GTACCGGGAA 
CCCTTGCGTT TATTTCCCCG TAAAGGGCCT CCCCTCCCCC GAGATGACAC AGGAGTCCAC 
CGTCTGGCTG CAGCCCGTGA CAGCCAGCGC AGGAGCTACA AGGTTCGTTT CAACAGTGTT 
TCCTCCTATT CCGACTCCCG CAAATCATCG GGCAATGGGC CGGACCTCAG AGACAACTTT 
GAGAGCACGG GACCCCTGGC GAATGTGAGG TTCTCCGTGT TCGGCCTTGG CTCGCGGGCG 
TACCCTCACT TCTGCGCCTT TGGACACGCC GTGGACACCC TCCTGGAAGA GCTGGGTGGG 
GAGAGGATCC TGAAGATGAA GGAGGGGGAT GAGCTCTGTG GGCAGGAAGA GGCTTTCAGG 
ACCTGGGCCA AGAAGGTCTT CAAGGCAGCC TGTGATGTGT TCTGCGTGGG AGATGACGTC 
AACATTGAGA AGGCAAACAA CTCCCTCATC AGCAATGACC GCAGTTGGAA GAGGAACAAG 
TTCCGCCTCA CCTACATGGC CGAAGCTCCA GAACTCACAC AAGGTCTATC CAATGTCCAC 
AAAAAGCGGG TCTCAGCCGC TCGACTCCTC AGCCGTCAGA ACCTTCAAAG TCCTAAATCC 
AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028335872.1
CDS	700..3825
Translation	MENHTFSVKQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGQPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTRPLGPPTSAVDLSHQPSASKEQTLAVDGATGPGNGPQHVPDQGQEAGLLSHTNGLAPRPPSQDPAKKSSGENSQLLREIEPVLNLLTSGSKGISGGGPARAETKDAEIQVDRDLDGKSHRPLPLGVENDRVFNDLWGKGNMPVVLNNPYSEKEQPPASGKQSPIKNGSPSKCPRFLKVKNWETDVVLSDTLHLKSTLGMGCTEHICMGSIMLPSQQIRRPEDVRTKEQLFHLAKEFIDQYYSSIKRFGSKAHLERLEEVNKEIETTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPASVEFTQICLQQGWKPPRSRFDILPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDSSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNSHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVHEERKYREPLRLFPRKGPPLPRDDTGVHRLAAARDSQRRSYKVRFNSVSSYSDSRKSSGNGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMKEGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYMAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSR

Target ORF information:

RefSeq Version	XM_028480071.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon nitric oxide synthase 1 (NOS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028480071.1

ORF Insert Sequence:

ATGGAGAATC ACACGTTCAG CGTCAAGCAG ATCCAACCCA ATGTCATTTC TGTCCGCCTC 
TTCAAGCGCA AAGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGAGGGTGAG CAAGCCACCT 
GTGATCATCT CCGACCTGAT TCGAGGGGGC GCCGCGGAGC AGAGCGGCCT CATCCAGGCT 
GGGGACATCA TTCTCGCCGT CAACGGCCAG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGAGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCC 
GAGGGCTTCA CCACGCACCT GGAGACCACT TTCACTGGGG ACGGGACCCC CAAGACCATC 
CGGGTGACAC GGCCCCTGGG TCCGCCAACC TCAGCCGTCG ATCTGTCCCA CCAGCCATCA 
GCCAGCAAAG AGCAGACCCT GGCAGTGGAC GGGGCCACGG GTCCTGGCAA TGGGCCGCAG 
CACGTGCCAG ACCAGGGGCA GGAGGCTGGC TTGCTCTCCC ACACCAATGG CCTGGCTCCC 
AGGCCCCCGA GCCAGGACCC TGCCAAGAAG AGTTCTGGGG AGAACAGTCA ACTGCTCAGA 
GAGATAGAGC CTGTGCTGAA TCTTCTCACC AGCGGGAGCA AAGGGATCAG CGGAGGGGGA 
CCTGCCAGAG CGGAGACAAA GGACGCAGAA ATCCAGGTGG ACAGAGATCT GGACGGCAAA 
TCACACAGAC CGCTGCCCCT CGGTGTGGAG AACGACCGCG TCTTCAATGA CCTATGGGGG 
AAGGGCAACA TGCCAGTCGT CCTCAACAAC CCGTATTCAG AGAAGGAGCA GCCCCCTGCC 
TCGGGAAAAC AGTCTCCCAT AAAGAATGGC AGCCCCTCCA AGTGCCCTCG CTTCCTCAAG 
GTCAAGAACT GGGAGACCGA TGTGGTTCTC TCTGACACCC TCCACCTTAA GAGCACCTTG 
GGAATGGGAT GCACCGAGCA CATCTGTATG GGGTCCATCA TGCTTCCTTC TCAGCAAATA 
CGAAGGCCTG AAGACGTCCG CACAAAAGAA CAGCTCTTCC ATCTTGCCAA AGAGTTTATT 
GATCAGTACT ATTCATCAAT TAAAAGATTC GGCTCCAAAG CCCATCTGGA GAGGCTGGAA 
GAGGTGAACA AAGAGATTGA AACCACCAGC ACCTACCAGC TCAAGGACAC AGAGCTCATC 
TACGGGGCCA AGCACGCCTG GAGAAATGCC TCCCGCTGCG TCGGCCGGAT CCAGTGGTCC 
AAGCTGCAGG TGTTCGATGC CCGCGACTGT ACCACGGCTC ACGGGATGTT CAACTACATC 
TGTAACCATA TCAAGTATGC CACCAACAAA GGGAACCTCA GATCTGCCAT CACCATATTT 
CCCCAGAGGA CCGACGGCAA GCATGACTTT CGAGTCTGGA ACTCCCAGCT CATCCGCTAT 
GCTGGCTACA AGCAGCCTGA CGGCTCCATC CTGGGGGATC CAGCCAGCGT GGAGTTCACA 
CAGATCTGCC TCCAGCAGGG CTGGAAACCC CCAAGAAGCC GCTTCGACAT CCTGCCACTC 
CTGCTCCAGG CCAACGGCAA TGACCCTGAG CTCTTCCAGA TTCCTCCAGA GCTGGTGCTG 
GAAGTGCCCA TCAGGCACCC CAAGTTTGAA TGGTTCAAGG ACCTTGGGCT GAAGTGGTAC 
GGCCTCCCCG CTGTGTCAAA CATGCTCCTG GAGATCGGTG GCCTGGAGTT CAGCGCCTGT 
CCCTTCAGCG GCTGGTACAT GGGCACAGAG ATTGGCGTCC GCGACTACTG TGACAGCTCC 
CGATACAACA TCCTGGAGGA AGTGGCCAAG AAGATGAACT TGGACATGAG GAAGACATCC 
TCCCTGTGGA AGGACCAGGC ACTGGTGGAG ATCAACATTG CGGTGCTCTA CAGCTTCCAG 
AGTGACAAAG TGACCATCGT TGACCACCAC TCTGCCACCG AGTCCTTCAT TAAGCACATG 
GAGAATGAAT ACCGCTGCCG GGGAGGCTGC CCCGCTGACT GGGTGTGGAT TGTGCCCCCC 
ATGTCTGGCA GCATCACCCC CGTCTTCCAC CAAGAGATGC TCAACTACCG ACTCACCCCC 
TCCTTCGAAT ACCAGCCTGA CCCTTGGAAC AGCCACGTCT GGAAAGGTAC CAACGGGACC 
CCCACAAAGC GGCGAGCCAT TGGCTTCAAG AAGCTGGCAG AAGCCGTCAA GTTCTCAGCC 
AAGCTAATGG GACAGGCCAT GGCCAAGAGG GTCAAGGCTA CCATCCTGTA CGCCACAGAG 
ACGGGCAAAT CGCAGGCTTA TGCCAAAACC CTGTGTGAGA TCTTCAAACA TGCCTTTGAC 
GCCAAGGTGA TGTCCATGGA AGAATATGAC ATTGTACACC TGGAACATGA AACTCTGGTC 
CTGGTGGTTA CCAGCACCTT CGGAAACGGA GACCCCCCTG AGAATGGGGA GAAATTTGGC 
TGCGCTTTGA TGGAAATGAG GCACCCCAAC TCTGTGCACG AGGAAAGGAA GTACCGGGAA 
CCCTTGCGTT TATTTCCCCG TAAAGGGCCT CCCCTCCCCC GAGATGACAC AGGAGTCCAC 
CGTCTGGCTG CAGCCCGTGA CAGCCAGCGC AGGAGCTACA AGGTTCGTTT CAACAGTGTT 
TCCTCCTATT CCGACTCCCG CAAATCATCG GGCAATGGGC CGGACCTCAG AGACAACTTT 
GAGAGCACGG GACCCCTGGC GAATGTGAGG TTCTCCGTGT TCGGCCTTGG CTCGCGGGCG 
TACCCTCACT TCTGCGCCTT TGGACACGCC GTGGACACCC TCCTGGAAGA GCTGGGTGGG 
GAGAGGATCC TGAAGATGAA GGAGGGGGAT GAGCTCTGTG GGCAGGAAGA GGCTTTCAGG 
ACCTGGGCCA AGAAGGTCTT CAAGGCAGCC TGTGATGTGT TCTGCGTGGG AGATGACGTC 
AACATTGAGA AGGCAAACAA CTCCCTCATC AGCAATGACC GCAGTTGGAA GAGGAACAAG 
TTCCGCCTCA CCTACATGGC CGAAGCTCCA GAACTCACAC AAGGTCTATC CAATGTCCAC 
AAAAAGCGGG TCTCAGCCGC TCGACTCCTC AGCCGTCAGA ACCTTCAAAG TCCTAAATCC 
AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NOS1 cDNA ORF clone, Physeter catodon(sperm whale)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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