HECTD4 cDNA ORF clone, Physeter catodon(sperm whale)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECTD4
Entrez Gene ID	102991601
Full Name	HECT domain E3 ubiquitin protein ligase 4
Gene Type	protein-coding
Organism	Physeter catodon(sperm whale)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007114335.1	XP_007114397.1	probable E3 ubiquitin-protein ligase HECTD4 isoform X1
XM_024120790.1	XP_023976558.1	probable E3 ubiquitin-protein ligase HECTD4
XM_028480292.1	XP_028336093.1	probable E3 ubiquitin-protein ligase HECTD4
XM_007114336.1	XP_007114398.1	probable E3 ubiquitin-protein ligase HECTD4 isoform X2

Pathways from BioSystems
Gene Ontology

The following HECTD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECTD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPh122637	XM_007114335.1 Latest version!	Physeter catodon HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OPh122638	XM_007114336.1 Latest version!	Physeter catodon HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OPh289407	XM_024120790.1 Latest version!	Physeter catodon HECT domain E3 ubiquitin protein ligase 4 (HECTD4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OPh325006	XM_028480292.1 Latest version!	Physeter catodon HECT domain E3 ubiquitin protein ligase 4 (HECTD4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECTD4 ( XM_007114335.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007114397.1 Physeter catodon HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPh122637
Clone ID Related Accession (Same CDS sequence)	XM_007114335.1
Accession Version	XM_007114335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	probable E3 ubiquitin-protein ligase HECTD4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006714665.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTATGGA CATGCTCGAA TGACTATATC GATCAATGGT GTAATCCTGG GAATCAGGCC 
TTCCATTACG TCTGTCAGAG ACTTGGAGTC AGCCATATCA TCACTGAGCC TAAAGAAGAG 
GCTATAACCA CGAATGAGGT TATAAACCAA TTATTGCACC ACGTTGGTGC GATGTGCATA 
CACCAACTCA ATCTTCTTGC TACCAACCCC AATCTTCCAA TCACAAGTGT CTTGGGCAAG 
CAGCATCCAA TTGAAGCACA CCATCTTAGC AGTATTTGTG ACATTATGGA GAAGGCCATG 
GTTAATGGAG ACACCTGTAT CATACGCTGC ATTCTCGTTG TCTTTCAGGT GGTGTTTAAA 
TTTTTCTTCA GCCCACAAAC TGAAAGGAAT CGAGACATCA TTCGACGGTC GGGGTTGCTT 
CTTTGGCAGT TGTTGATGGC ACCAAAAGAT CAAATTTGTC CTGAAATTCA GAAGGAAGTC 
TGCCTTGCCA TCAGCTCTGG TTTAAATATC TTGTACCCAG GTGAAACTGA AATCAATAAC 
TTACTTAAGC TGGTCTTAAC AGAAGGAGAG AGAAACAGTG GCCTCTCCCA GCTGCGGGAT 
GTGATCCTAA CCAATTTAGC TGAACAGCTT CAAAACAACC GATTTGGCAG TGAGGAGGAT 
GATCATTACA GACTAAATGA CGAACTTTTA CACTACATTT TGAAGATTGT TGTACGAGAA 
TCCTGTATCT TAATCACCAA GTGCCAAACT GTCTCTAAAG ATGATTTTCA AAAACTCCTT 
TCAACTGTGC CTGCTGCATC CTCCTGCCTG CGCTATCTGA TGGCAGTTCA GAACCACCTT 
CTCAGTAACA CTGTTTTGAT TAAACCTGAT GAGAATGATG ACAGTGACAA CTCCTTGCAG 
GGAGAGACAT TGAAGGTACA GGAGCTAAAG GTCAGTATTT TGGCTCTTGC CACCCAAATC 
CTGACTGGAT GTGATGAAGT GTTGGAAATG CTACAGCAGG TCACAACTGC CCTCATAAAT 
AGTGACATAG CTGACCGGGA GCAGAGGTTA AAAGGCTTGG AACAAGTTAC TAAGGCTACT 
ATGCTCGGTC ACCTTCTTCC TGTGTTACTG ACCTCCCTGA TGCATCCAAA TTTACAGACT 
CTGACCATGG CCGATGCCCT GATGCCTCAG CTAGTGCAGC TGGTACTTTA TACCAGTCAG 
ACGGCTTTGC TGCTCAAAAC CCAGTGTCCG GTTTTTGCTG AGGCGGGCTG TTCCCCATGT 
GGTGCATCAG ACCAGAAGGG CAGGCTGTTC CCTGATGAGA GAATGCTGGA AGAGAAGGAA 
GAGCCAGGGT TTCTCACTGG TTTAAAGATT CCTGCTCCAT GGGCTGCTGG GAAGACTGTG 
GAAACAGTCC ATCCTGTCAG AGACAACTAT AAATTTAAAG AAACTGTCCA TATCCCGGGA 
GCTCGCTGCC TATACCTTAG ATTTGATAGC AGATGCTCTT CACAATATGA CTATGACAAA 
TCTAACAGTA TATTTTCTGT TTTGAAGTTG GTGATATACG CGGGGCCTAA CACAAACAGT 
AGGAAGGTTG CTGAGTATGG AGGCAACACT CTGGGATATG GCAGCCGTAG TGTCTTAGGA 
ACTGGTTGGC CGAAAGACTT GGTGAAGGTG GAAGGAGATA CAGTCACCTT CTCCTTTGAA 
ATGAGAAGTG GCCGTGAACA CAACACTCCC GATAAAGCTA TGTGGGGCTT TGCTTGCACA 
GTTCGCGCTC AGGAGTCTTC AGAGGATGTG TCGGGAGGCT TACCTTTTCT GGTAGACCTG 
GCTTTAGGTC TGTCTGTGTT AGCTTGTTCT ATGTTAAGAA TCCTATACAA CGGACCAGAA 
ATTACCAAAG AAGAAGAAGC CTGTCAGGAG CTGCTGCGGT CCAAACTTTT ACAAAGGTGC 
CAGTGGCAGG TGGAGGCCAA TGGTGTGATC TCCCCTGCCC TTACTCCAAG TCCGTCTCCA 
CTGCCTCTGA CCATCGAGGA AGACAGAGAA TTCACTTACC CCTCTGACGT CCTCGTGCCT 
CCTGTTGGAA ACTACTTTGA CCTGCCCCGG ATCAGGCTGC CTCCAGGAAT CATGATAAAG 
CTCAGGGAAA TTTCTGGGCG TGCCAGACCT CAATTTAGAC CAAGTATAAA AGAAGTAATC 
CAGCCAGACG TGATGGAGGA AATGGTGGTA TCGTGTGTCA TTAAGCACTT GAACTTGGTT 
GATGCACTGC AGTCCCTAAT AAATTTCCAG TATCAAGAAG AACATGCTGA GGAATATGAT 
TTATTGTGTA AAATCATGGG AGAGACATTT AAGAAACTCA ATGCCATGGA GAGACAGCTG 
CAGAGTGTTG CAGAATTGGA ACAGAAATGG CAAAGTGAGG TTGATGATGC CACGCAGGGG 
AAACTGGAGA ACAACATGCC TTTCTTCTAT GATTACCATT TTAATGAGAA CAAAATGAAA 
GAACTAGAAC TTTTGTGTTC AATGAAGGAA GTCTCCTTTG ATGGAAATGA TCTTGAAAAC 
ATGGTCCTAT CTCTGAGGGA GAAGTTCCTA CAAGAAGTGA ATTCTCTCAT TCAGAAACCC 
TCGCACCCAC TGGCTAAGAC GAAGACGTTA GTGAAGAGTC TAATGAACCG AGCTGAGCTG 
TTGCTGCACG TCACCATTGC GGCCCAGTCC GGCCTCACAA GAAGCATCTC AGGGACCCCC 
GCAGAAACAC CGGCTTGTAA ATCAGCCTCT GAAACAAAGG TGATATCTCA TGCTGTGAGG 
CAGCCTGTTT TTCTTCGCAG CATGTCGGCT CCTTCTGACC TGGAAATGAT TGGTAACGAT 
GATTTGGAAT TTACTAGAGC AAATCAGAGG CGGCGCCACG TGACCAGCCA CCGCAGCAGC 
TCCTTCACGC TCCTGCAGTC ATTGGCCATC GAGGACAGCA GGGACAAGCC CACCTACAGC 
GTCCTGCTGG GGCAGCTGTT TGCTTTCATC GGCACCAACC CTGACCAAGC TGTGTCCAGC 
AGCAGCTTTC TTTTGGCTGC GCAGACAAGG TGGCGACGGG GAAACACTCG CAAGCAGGCA 
CTGGTGCACA TGCGGGAATT GCTGACGGCT GCCGTGCGAG TCGGGGGAGT GACACATCTT 
GTGGGTCCGG TGACTATGGT CCTTCAGGGA GGACCCAGAA TTGAAGAACT AACCTGTGGT 
GGGATGGTCG AGCAGGTCCA GGAAGCCTTC GGTGAGACCA TGACCTCTGT TGTGTCTTTG 
TGCGCCCGTT ACCCTATTGC TTGTGCGAAT AGCATCGGAC TCTTATGTAC TATACCTTAC 
ACCAGGAGTG AAGAGAAGTG CCTGGTGCGC AGTGGCCTTG TTCAGCTCAT GGATCGACTG 
TGTAGCTTGA GCAGTCAGAC TGAGTCCAGC TCCAGTGAGA AACAAACCAA GAAGCAGAAG 
GTGGCTACCA TGGCCTGGGC AGCCTTCCAG GTGCTTGCCA ACCGCTGCGT TGAATGGGAA 
AAAGAAGAAG GCGGCTCCAC AGAGGCTGTG CACTCAGGTC TGGCCCGTCA GGTGTCCAGC 
CTTCTCACTA ACCACCTCGC CCGAGCGACG GAGAGCTGCG GCAACCAGGC TGCCGGGAAC 
GATGCCCTCC AGGACGTCCT GAGTCTTCTC AATGACCTCT CGAGGAGTCA CATAGGTAAA 
GCCATCCTGA GCCAGCCAGC TTGTGTGTCC AAACTCCTCT CCCTGCTGCT TGACCAACGC 
CCGTCCCCAA AGCTGGTCCT TATCATTCTT CAGCTGTGCC GGGCAGCACT CCCGCTGATG 
AGCGTAGAAG ACTGTGGGAA TGTGGAGCTC CCACCCTGGA GCTACTCTGT CCCCTCCCTA 
AACAGTGAGC AAGAGGATCC CAGCGACCCA GCGTCCAAGA TCGCCTCTTT GCTCTTAGCA 
AAGCTAGCAG ATTATGTGGT CCCAGGATGT CAGACAGTCC TTTCTCCAAC CGCTTCTGAA 
CCTGACACCA TGTTGACAAA AACCAGCCCC AAGAGTTCCT TAAAAGGAGA TAAAGACCCT 
GGAGAAGAGA GTGAAGCAGT GGATGGCAAG CTCTCTATCT TTATCCACAA GCGGGAAGAC 
CAGTCATCCC ATGAAGTTCT CCAACCATTA CTAAGTAGCT CAGAAGGACG GCCCTTCCGA 
CTTGGTACTG GTGCCAACAT GGAGAAAGTC GTGAAAATGG ACCGAGACAT GACAAAGGGT 
GGCTGCTGTG AGGTGATTAC AGAAGAGGCG GCAGCGGCTC TGCGAAAAGC AACCAAGTGG 
GCACAGTCTG GCCTCATCGT CAGCGTCGGG CCACCCGTGG AATCGCTCAA CCCAGAGACT 
GTGAGTGGAC TGTCCACAGG GGACAAGAAG AAAACCGCCC AAACTTCCAT TTGCCGAGAG 
AGGAACTCTG AACTTGCCAG GACCGACCCT GTCCGACCTT TCATCAGTGG GCATGTGGCA 
AACAGCATGG CTGCAGAGGT GATCGCCTTG CTTCACAGCT TGCTGATGGC GCCTGAGTCA 
AATGCTGCTC AGATATGGAC CACGACAGCA GAGAAGGTTC TGTCTCGTGC GCTGATGTAC 
ATTCCACAGT TGGGGAAATA TGCAGAAAGC ATTCTGGAAA ATGGCAGCAG TAGTGGCAGA 
AAACTCGCCA AACTTCAGAG AATCGCCCGC CAGGCTGTCG CTGCACTGTG TGCACTTGGA 
GGCTTCAAAG AGACAATCAA GATAGGGTCT GAGGTTCAGG TTTTAGGTAG AGGAATATCA 
GGAAGTGTCG GAGTAGTGGC TTCTATCAAT GAACAGGAAG GTATAGCTAC AGTCAAATTC 
CCACCCATAG ACTGTAGAAA GACGTCGCAA GCATCAGACA CACTGACTAT TCCATTGTCT 
AGACTTTGTG TTCCAAGATC AGAGGCATTG CCTCTTCATA AACTGTCCAT TACTGAGAAG 
GTAGTACAGG CAGTCCAGTC CATGTTGCTT CCTCAGGAGG GAAGTCTCTC TATTCATACC 
TCACTTCCTG CAACAGGAGA TGGGTCAGCT CCTGTGATGG CAGTCGTTCG GCTCCTGGCT 
GAAATAAGAA CAAGAGCATG CCTGGTCATG GCCCAGCTTT TAGAAGACAG CTTATTTTGT 
GAAGAATTCA TACAGCAGTG TCCAGCAGCT GTTGAGGTTC TCAACCTAGT AGCCCAGGAA 
TGCAGTGCTG GAGAGCGACT GGCAGTTGTG GAAGTGCAGT GTGAACGGCT GAGGATGCTC 
TACCGGGACT GTGCTCGGCC CCCGCCACCT CCGCTGCAGG CTGACCGGAG ACAGCCCAAA 
GAGATTACGT GGAGCCCCTC ACGAGTGTTT CCACCTGTCC GAGCCTGCAT GTTCTCATCG 
CACCTCACTT CCGTCACCTT CCTGGCTGAC CCCAGCGCTG GGGGCGGTCT GCCCCGGGGC 
ACATTTATCT ATGCCACCTC ACCACTGCCC ATTCAGGCCC CATCTTTCTA CTGGGAAATT 
GAAATTGTTT CCTATGGAGA TAGTGATGAT GACACTGGAC CAATAGTTTC CTTTGGCTTT 
GCTACAGAAG CAGAGAAAAG AGACGGGGCT TGGACTAATC CAGTGGGCAC TTGTCTTTTC 
CATAACAATG GCCGAGCCGT GCACTACAAC GGCTCCAGTT TATTACAGTG GAAGAGCGTT 
CGCTTAGACG TGACTCTCTC TCCTGGTGAC GTGGCAGGAA TTGGCTGGGA GAGAACCGAG 
GGAACGCCTC CTCCTCCAGG GCAGCCAGCC AAGGGCCGAG TGTACTTCAC ATACTGCGGG 
CAGCGCCTCT CACCGTATCT TGAAGATGTT TCGGGTGGGA TGTGGCCTGT GGTTCACATT 
CAGAAAAAGA ACACCAAGAC GCGAGCTAAC TTTGGCTCCC GCCCATTTGC CTATGCTGAA 
GGGCAGGCCC ACCGCAACGC AGCTGACCTG TGCACCGACC TGGCAGAGGA GATCAGCGCT 
AACTTCGAGG CCCTGCCCTT TGCCATGGCA TCTGACAGTG ACAACGATGC TGGCACCAGT 
ATTGCGTCAG ACCCGGGCAC TCACGGGCCA CCGTGCCGGA TTGCTGCCGT GGCCACGGCT 
CAGCAACAAT ATGATAGTGA CACTTCCTGT CATTATAAAG TAGAGCTCAG CTATGAGAAT 
TTCATCACCT CCGGCCCAGA CCCCCATCCG CCCCCCATTG CAGATGACGA AAGTGATGAT 
GATGATGACG ATGATATCCC GCAGGAGGAC CACTATGCTC TACTGGTGAA AGCGTGGGAG 
ACCAAGGTTT TTCCTACTAT CCGAAGACGC TTCCGCAACG AAGCAGAGCG GAAATCGGGC 
CTGGACCAGA TAAAGGGTGC TTTACAACTA GGCATGGTGG ATATTGCCCG GCAGACAGTT 
GAATTCCTCT ACGAGGAGAA CGGTGGCATC CCCAGAGACC TTTATCTTCC CACCATTGAA 
GACATTAAGG ATGAAGCAAA CAAGTTCACA ATTGATAAAG TTCGAAAAGG TCTCACGGTA 
GTAACCCGCT CTCCAGACAG CAATAACGTA GCCAGCAGTA CTGTTGGAAC TGCCCTGCCA 
AAATTTGCCA TCCGAGGGAT GCTGAAAACC TTTGGGCTTC ATGGAGTTGT CTTAGATGTT 
GATTCAGTAA ATGAATTGGT GCAGGTAGAA ACGTACCTCC GAAGCGAAGG TGTGCTGGTC 
CGATACTGGT ATCCTATTGA TATGTTGGAA AGGCCCCCAG CAGGCTACCG AAGGACTGCC 
ACCAATGGGC TGGTCACCCT GGACAATACC AACCTTCAAA TTCACAGGGA GCTGCTGCGC 
TGCGAGGCCG CACTGGCCAG GCTGTACTGC CGCATGGCCC TGCTCAACAT CTTCGCCCCC 
AGGCTGCCTC ACCTGTTTAC CCGCCTCTTC CACATCCCTG CCATCCGGGA CATTACCCTG 
GAGCACCTGC AGCTGCTGTC CAATCAGCTC CTCGCTCCTC CCCTCCCAGA TGGCACCATC 
AGCTCCAGCT CCATCCTCCT GGCACAGTCG CTGCAGCACT GCATCCATTC CCAGAACTGC 
TCGGCCACGG ACCTCTTCTA CCAGGGCAGC TCCCAGACAG TGAGAGAGTG GCTCAACGTG 
GCCATCACTC GGACCCTGCA CCAGGGTGAG GAGAGCCTGT TGGAGCTGAC GAAACAGATC 
TGCTCTTTCC TGCAGACGGC ACCAGAGCAG TTCCCCTCCG AAGAGTTTCC AATTTCCGAG 
TCCAAGGTCA ACATGGACGT CAATTTCCCC GGGGCGGCTT TCGTCGTGGT ATCTTGCAAA 
GAAAGTCAGT CTGGATTCCG CAAAGACTCC TCTCTGTACA AGGCGCCGTG GGCGCGCGTG 
CTCGTGTACG GACTGGGCCA CAAAGTGAAG CGAAATGGCC AGCTAAACCT CATCGAGGCC 
ATCTGTTACC CGCGGGATGC GTCTCCGGCC AACACGGGGC TCACGCCGCC TCCCACCACC 
AACCAGTACC CTTCTGTGAT CCTCTCCACA GACAAAGTCC ACATCAAACT GGGGGTGTCT 
CCCCCTCCTG GAGCCGTGCT GGTGTTACAC TCTCTGCCCC TTGAGTTCCC ACTGGCGATG 
GCCTTCGCAG AGCAGCTGCT GTCCTGGAAG TCGGAGGACG GTGAAGGGAG ATCTGAAGAC 
GAGCCTGACA CCATCCCGAC GTCCGTCCTC CTGCAAGTGG TGGAACTGCT CGGAAACTTC 
CTGTGGACCA CGGACATGGC GGCCTGTGTG AAAGAGCTGG TTTTCCACCT CCTGGCAGAG 
CTCGTGCGCA CAGTGCACAG CCTGGAGCAG AGGAAGCACC CTGCCGGCCT GTCCTCGTCC 
ATCGCCCTCC AGCTGAACCC CTGCCTGGCC ATGCTGATGG CCCTGCAGTC AGAGCTCCAC 
AAGCTGTACG ACGAGGAGAC GCAGAGCTGG GTCTCGGGCA GCACGTGCGG AGGCTCGGGG 
ACCGTGGCGG CCACCGACCA GGGCAGGTTC TCTACGTATT TCCACGCGCT CATGGAAGGG 
TGCCTGGCGG TGGCCGAGGT GACCCTGCCT ACCAACATGA GCGTCACTGC CAGCGGGGTG 
ACGTCGGCGA CCGCCCCAAA CCTCAGTGAC TCGTCGTCCT CCTCGTCATC CTCCCCAGGA 
CAGACGCCGC AGAGCCCCAG CCTCCTGTCC AAGAGGAAGA AAGTCAAGAT GAAGCGGGAA 
AAGGCCTCCT CCTCTGGGAA GCGCCAGTCC TCGCACTCAG TGGACTCGGA CCCCGCCGTG 
CTGAGCATCG GGGGTGGCAA GCCCGAAGAC ATGCTGTGGT TCCATCGCGC CCTGACCTTG 
CTCATCATCC TCCGGCACCT CACCAGGAAG GACCCGCAGG GGCTGGGTGT GACGAGCGAC 
GCCATCGCCG ACGCCTGCCA GGCCCTGGTG GGTCCCACCG CCCACAGCCG CTTGCTGGTG 
ATCTCTGGGA TCCCCACCCA CCTGGACGAG GGCATCGTCA GAGGCGCCAT CCGCAAGGCC 
TGCAACGCCC ACGGCGGGGT CTTCAAAGAC GAGATCTACA TCCCCCTGCA GGACGAAGAC 
CCCAAGAAGC CAAAAGACAA GGCCGAGGGC GGTGATGGGA AAGCCGAGCC AGAGAAGACC 
CTTGGCTTCC CCAGCACAGA CAGCTTGGAG GTCAGCACGT CCCGCAGCCC GACCCCCGCC 
ATGAGCATCA GCGCGTCTGC GTCCACCAGC CAAGCCTCCA TCTGCAGCTC CCAGGGCATC 
TTGCAGACAG TCAGCGATGT CTCTGTGGAA CCGCTGCCCT CGGGCCTCGA GCTGCCCATC 
CCTGCGGGCT TATTGGAGCC CCACGTAGTG TCCAGCCAGG AGAGCCTGGA CGTTTCCCTG 
TGCAGCACCA GCAGCCTGGG CAGCCTCGGG AGCCTGGGGG AGCCACTGGA CAACGCAGAA 
ACGGCCTCAG TGTCAGACAT GGGCTCCATG TACACAGTCA CGTCCCTGGA CAACCAGCCC 
CTCGCAGCAC GCCCCATCAA AGGCTTCGCG GTCGTGGAGA TTAGATCCCG AGCCAAAATC 
GAGAAAATCC GAGCGAGTTT ATTTAACAAT AATGACTTGA TTGGTTTGTC AAGTTTGGAT 
GGTGAAGATG AATTGATGGA AATGTCAACG GAAGAGATCC TCACTGTGTC TGTAGTAAAT 
CAGAGTTTGT TTGACACGCA GGGGAGCCCA GGGTTAGAAG ATTATTTCAA TGATAAGTCA 
ATTAAAGGTG AGAAGCTGGT GCCCGGGGCC AGAGAGGTGC TGACGGAGAT ATTTAAGAGC 
TGCGCCCACT CAGAGCAGAT GCTGAGTCTG ACACCAGCAA AGCCCATCAA AGTGTCTGAC 
ATTTATCTCA GCAAAGAGCA GATCAACTCC CAGACGCCAG GCAACCTCCT CCACCTCTTC 
TTCACCAATG TTCGGCCCCC CAAAAAGGTG CTGGAGGACC AGCTCACCCA GATCCTGAGG 
AAGTACGGCG TGCCAAAACC CAAGTTCGAC AAGAGCAAGT ACAGCAAGGC GGGGAAGGAA 
CAGCACCCCG GGAAGGTGGT GAGCACCAAG CGGCCCATCA CCAAGCCACC TGCCAAGGAC 
AAGGCCGTGC TCAACAGCGT CAGCAGGACT GCCTTGAGCG AGAAGAAGCC CACTGTGAAG 
CCGAAGTCAC CGGAAAAGAG CAAGCCGGAC GAAAAGGACC CGGAAAAGTC ACCCACCAAA 
AAACAAGAAG TCCCTGAGGA AAAGTACCTG ACATTGGAAG GATTTCACAA GTTTGTTATT 
GACCGAGCCA AGCAAGACAT CCGTAGTGTC TGGAGGGCGA TTTTATCCTG TGGTTACGAT 
CTTCATTTTG AGAGGTGTGC CTGCATCGAC GTCCGGCACG CACACAAGGC CTCCAGGAAG 
TGGACCCTGG AGATGGACGT GGCGCTCGTG CAGTACATCA ACCGGCTGTG CCGCCACCTG 
GCCATCACGC CCGCGCGGCT GCATCCCCAC GAGGTGTATC TGGAGACCAC AGACGCCGCC 
GACCCCAGAG TGGCCTGTCT CTTGAATGTG CCCATTGAGA GCCTGCGCCT GCGCTTCGCC 
CTGCTGCAGT CCCTGAACAC CACGCTCGAG ACCTTCTTCC TGCCACTCGT GGAGCTGCGC 
CAGACGCCCA TGTACACGCA CAGCATCGCG GCCCTGCTGA AGGAGGCCAA AGGGCTGATC 
TTTTATGACA CGAAGGTGAC CGTCATGAAT CGGGTGCTGA ACGCCACAGT GCAGAGGACG 
GCTGACCACG CGGCACCGGA GATTACTCTG GACCCACTGG AGATCGTGGG AGGGGAGATC 
AGAGCCTCTG AAAACTCCTA CTTCTGCCAG GCGGCCAGGC AGCTGGCCTC GGTGCCATCC 
TCCCAGCTTT GCGTCAGACT GGCGAGTGGC GGTGACCCCA CCTATGCCTT CAACATCCGC 
TTCACTGGGG AGGAGGTCCA CGGCACCAGC GGCTCCTTCC GCCACTTCCT GTGGCAGGTG 
TGTAAAGAGC TGCAGAGCTC CGCGCTCTCC CTTCTCCTGC TGTGCCCCAG CTCAGCCGTC 
AACAAGAACA AGGGCCAGTA CATCCTGAGC CCAAGCCCCA TCAGCTACGG GGAGGAGCAG 
CTGCTGCACT TCCTGGGCCA GTTGCTGGGA ATTGCGATTC GAGCAGATGT CCCTCTTCCC 
CTGGACCTCC TACCCTCCTT CTGGAAGACG CTGGTGGGCG AGCCCCTGGA CCCTGACCAA 
GACCTGCAGG AAGCAGACAT ACTCACCTAC AATTATGTCA AGAAATTCGA GAGTATCAAC 
GACGAGAGCG AGCTGGAGGC CCTGTGCGCC GAGATTGCCT CCCAGCACCT GGCCATCGAG 
AGCCCCGACG GGCCCAGTAA GCCCTGCTGC CGGTTCACCT ACCTGACCAT GGCGGGCGAG 
GAGGTGGAGC TGTGCAGCCG CGGCCGGCAC ATCCCTGTGG CGTGGGAGAA CAAGGACATC 
TACGCAGCAG CCATCCGGAG CCTGCGGCTG CGGGAGCTGC AGAACGTCGA GTGTGTGACA 
GCCGTGCGCG CGGGCCTCGG CGCCATCATC CCCCTGCAGC TGCTCACCAC GCTCAGCCCG 
CTGGAGATGG AGCTGCGGAC GTGCGGCCTC CCTTACATCA ACCTCGAGTT CCTGAAGGCC 
CACACCATGT ACCAGGTGGG CCTGATGGAG ACGGACCAGC ACATCGAGTT CTTCTGGGGG 
GCACTGGAGA TGTTTGCCCA GGAGGAGCTG TGCAAGTTCA TCAAGTTTGC CTGCAACCAA 
GAGCGCATCC CGTTCACCTG CCCCTGCAAA GACGGGGGCC CCGACACAGC CCACGTGCCC 
CCATACCCCA TGAAGATCGC CCCCCCGGAT GGCACAGCAG GTTCCCCAGA CTCTCGCTAC 
ATCCGAGTGG AGACCTGCAT GTTCATGATC AAGCTTCCCC AGTACTCCTC TCTGGAAATC 
ATGCTGGAGA AACTTCGTTG TGCCATTCAC TATCGCGAAG ACCCCCTGAG TGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007114397.1
CDS	331..11787
Translation	MLWTCSNDYIDQWCNPGNQAFHYVCQRLGVSHIITEPKEEAITTNEVINQLLHHVGAMCIHQLNLLATNPNLPITSVLGKQHPIEAHHLSSICDIMEKAMVNGDTCIIRCILVVFQVVFKFFFSPQTERNRDIIRRSGLLLWQLLMAPKDQICPEIQKEVCLAISSGLNILYPGETEINNLLKLVLTEGERNSGLSQLRDVILTNLAEQLQNNRFGSEEDDHYRLNDELLHYILKIVVRESCILITKCQTVSKDDFQKLLSTVPAASSCLRYLMAVQNHLLSNTVLIKPDENDDSDNSLQGETLKVQELKVSILALATQILTGCDEVLEMLQQVTTALINSDIADREQRLKGLEQVTKATMLGHLLPVLLTSLMHPNLQTLTMADALMPQLVQLVLYTSQTALLLKTQCPVFAEAGCSPCGASDQKGRLFPDERMLEEKEEPGFLTGLKIPAPWAAGKTVETVHPVRDNYKFKETVHIPGARCLYLRFDSRCSSQYDYDKSNSIFSVLKLVIYAGPNTNSRKVAEYGGNTLGYGSRSVLGTGWPKDLVKVEGDTVTFSFEMRSGREHNTPDKAMWGFACTVRAQESSEDVSGGLPFLVDLALGLSVLACSMLRILYNGPEITKEEEACQELLRSKLLQRCQWQVEANGVISPALTPSPSPLPLTIEEDREFTYPSDVLVPPVGNYFDLPRIRLPPGIMIKLREISGRARPQFRPSIKEVIQPDVMEEMVVSCVIKHLNLVDALQSLINFQYQEEHAEEYDLLCKIMGETFKKLNAMERQLQSVAELEQKWQSEVDDATQGKLENNMPFFYDYHFNENKMKELELLCSMKEVSFDGNDLENMVLSLREKFLQEVNSLIQKPSHPLAKTKTLVKSLMNRAELLLHVTIAAQSGLTRSISGTPAETPACKSASETKVISHAVRQPVFLRSMSAPSDLEMIGNDDLEFTRANQRRRHVTSHRSSSFTLLQSLAIEDSRDKPTYSVLLGQLFAFIGTNPDQAVSSSSFLLAAQTRWRRGNTRKQALVHMRELLTAAVRVGGVTHLVGPVTMVLQGGPRIEELTCGGMVEQVQEAFGETMTSVVSLCARYPIACANSIGLLCTIPYTRSEEKCLVRSGLVQLMDRLCSLSSQTESSSSEKQTKKQKVATMAWAAFQVLANRCVEWEKEEGGSTEAVHSGLARQVSSLLTNHLARATESCGNQAAGNDALQDVLSLLNDLSRSHIGKAILSQPACVSKLLSLLLDQRPSPKLVLIILQLCRAALPLMSVEDCGNVELPPWSYSVPSLNSEQEDPSDPASKIASLLLAKLADYVVPGCQTVLSPTASEPDTMLTKTSPKSSLKGDKDPGEESEAVDGKLSIFIHKREDQSSHEVLQPLLSSSEGRPFRLGTGANMEKVVKMDRDMTKGGCCEVITEEAAAALRKATKWAQSGLIVSVGPPVESLNPETVSGLSTGDKKKTAQTSICRERNSELARTDPVRPFISGHVANSMAAEVIALLHSLLMAPESNAAQIWTTTAEKVLSRALMYIPQLGKYAESILENGSSSGRKLAKLQRIARQAVAALCALGGFKETIKIGSEVQVLGRGISGSVGVVASINEQEGIATVKFPPIDCRKTSQASDTLTIPLSRLCVPRSEALPLHKLSITEKVVQAVQSMLLPQEGSLSIHTSLPATGDGSAPVMAVVRLLAEIRTRACLVMAQLLEDSLFCEEFIQQCPAAVEVLNLVAQECSAGERLAVVEVQCERLRMLYRDCARPPPPPLQADRRQPKEITWSPSRVFPPVRACMFSSHLTSVTFLADPSAGGGLPRGTFIYATSPLPIQAPSFYWEIEIVSYGDSDDDTGPIVSFGFATEAEKRDGAWTNPVGTCLFHNNGRAVHYNGSSLLQWKSVRLDVTLSPGDVAGIGWERTEGTPPPPGQPAKGRVYFTYCGQRLSPYLEDVSGGMWPVVHIQKKNTKTRANFGSRPFAYAEGQAHRNAADLCTDLAEEISANFEALPFAMASDSDNDAGTSIASDPGTHGPPCRIAAVATAQQQYDSDTSCHYKVELSYENFITSGPDPHPPPIADDESDDDDDDDIPQEDHYALLVKAWETKVFPTIRRRFRNEAERKSGLDQIKGALQLGMVDIARQTVEFLYEENGGIPRDLYLPTIEDIKDEANKFTIDKVRKGLTVVTRSPDSNNVASSTVGTALPKFAIRGMLKTFGLHGVVLDVDSVNELVQVETYLRSEGVLVRYWYPIDMLERPPAGYRRTATNGLVTLDNTNLQIHRELLRCEAALARLYCRMALLNIFAPRLPHLFTRLFHIPAIRDITLEHLQLLSNQLLAPPLPDGTISSSSILLAQSLQHCIHSQNCSATDLFYQGSSQTVREWLNVAITRTLHQGEESLLELTKQICSFLQTAPEQFPSEEFPISESKVNMDVNFPGAAFVVVSCKESQSGFRKDSSLYKAPWARVLVYGLGHKVKRNGQLNLIEAICYPRDASPANTGLTPPPTTNQYPSVILSTDKVHIKLGVSPPPGAVLVLHSLPLEFPLAMAFAEQLLSWKSEDGEGRSEDEPDTIPTSVLLQVVELLGNFLWTTDMAACVKELVFHLLAELVRTVHSLEQRKHPAGLSSSIALQLNPCLAMLMALQSELHKLYDEETQSWVSGSTCGGSGTVAATDQGRFSTYFHALMEGCLAVAEVTLPTNMSVTASGVTSATAPNLSDSSSSSSSSPGQTPQSPSLLSKRKKVKMKREKASSSGKRQSSHSVDSDPAVLSIGGGKPEDMLWFHRALTLLIILRHLTRKDPQGLGVTSDAIADACQALVGPTAHSRLLVISGIPTHLDEGIVRGAIRKACNAHGGVFKDEIYIPLQDEDPKKPKDKAEGGDGKAEPEKTLGFPSTDSLEVSTSRSPTPAMSISASASTSQASICSSQGILQTVSDVSVEPLPSGLELPIPAGLLEPHVVSSQESLDVSLCSTSSLGSLGSLGEPLDNAETASVSDMGSMYTVTSLDNQPLAARPIKGFAVVEIRSRAKIEKIRASLFNNNDLIGLSSLDGEDELMEMSTEEILTVSVVNQSLFDTQGSPGLEDYFNDKSIKGEKLVPGAREVLTEIFKSCAHSEQMLSLTPAKPIKVSDIYLSKEQINSQTPGNLLHLFFTNVRPPKKVLEDQLTQILRKYGVPKPKFDKSKYSKAGKEQHPGKVVSTKRPITKPPAKDKAVLNSVSRTALSEKKPTVKPKSPEKSKPDEKDPEKSPTKKQEVPEEKYLTLEGFHKFVIDRAKQDIRSVWRAILSCGYDLHFERCACIDVRHAHKASRKWTLEMDVALVQYINRLCRHLAITPARLHPHEVYLETTDAADPRVACLLNVPIESLRLRFALLQSLNTTLETFFLPLVELRQTPMYTHSIAALLKEAKGLIFYDTKVTVMNRVLNATVQRTADHAAPEITLDPLEIVGGEIRASENSYFCQAARQLASVPSSQLCVRLASGGDPTYAFNIRFTGEEVHGTSGSFRHFLWQVCKELQSSALSLLLLCPSSAVNKNKGQYILSPSPISYGEEQLLHFLGQLLGIAIRADVPLPLDLLPSFWKTLVGEPLDPDQDLQEADILTYNYVKKFESINDESELEALCAEIASQHLAIESPDGPSKPCCRFTYLTMAGEEVELCSRGRHIPVAWENKDIYAAAIRSLRLRELQNVECVTAVRAGLGAIIPLQLLTTLSPLEMELRTCGLPYINLEFLKAHTMYQVGLMETDQHIEFFWGALEMFAQEELCKFIKFACNQERIPFTCPCKDGGPDTAHVPPYPMKIAPPDGTAGSPDSRYIRVETCMFMIKLPQYSSLEIMLEKLRCAIHYREDPLSG

Target ORF information:

RefSeq Version	XM_007114335.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007114335.1

ORF Insert Sequence:

ATGCTATGGA CATGCTCGAA TGACTATATC GATCAATGGT GTAATCCTGG GAATCAGGCC 
TTCCATTACG TCTGTCAGAG ACTTGGAGTC AGCCATATCA TCACTGAGCC TAAAGAAGAG 
GCTATAACCA CGAATGAGGT TATAAACCAA TTATTGCACC ACGTTGGTGC GATGTGCATA 
CACCAACTCA ATCTTCTTGC TACCAACCCC AATCTTCCAA TCACAAGTGT CTTGGGCAAG 
CAGCATCCAA TTGAAGCACA CCATCTTAGC AGTATTTGTG ACATTATGGA GAAGGCCATG 
GTTAATGGAG ACACCTGTAT CATACGCTGC ATTCTCGTTG TCTTTCAGGT GGTGTTTAAA 
TTTTTCTTCA GCCCACAAAC TGAAAGGAAT CGAGACATCA TTCGACGGTC GGGGTTGCTT 
CTTTGGCAGT TGTTGATGGC ACCAAAAGAT CAAATTTGTC CTGAAATTCA GAAGGAAGTC 
TGCCTTGCCA TCAGCTCTGG TTTAAATATC TTGTACCCAG GTGAAACTGA AATCAATAAC 
TTACTTAAGC TGGTCTTAAC AGAAGGAGAG AGAAACAGTG GCCTCTCCCA GCTGCGGGAT 
GTGATCCTAA CCAATTTAGC TGAACAGCTT CAAAACAACC GATTTGGCAG TGAGGAGGAT 
GATCATTACA GACTAAATGA CGAACTTTTA CACTACATTT TGAAGATTGT TGTACGAGAA 
TCCTGTATCT TAATCACCAA GTGCCAAACT GTCTCTAAAG ATGATTTTCA AAAACTCCTT 
TCAACTGTGC CTGCTGCATC CTCCTGCCTG CGCTATCTGA TGGCAGTTCA GAACCACCTT 
CTCAGTAACA CTGTTTTGAT TAAACCTGAT GAGAATGATG ACAGTGACAA CTCCTTGCAG 
GGAGAGACAT TGAAGGTACA GGAGCTAAAG GTCAGTATTT TGGCTCTTGC CACCCAAATC 
CTGACTGGAT GTGATGAAGT GTTGGAAATG CTACAGCAGG TCACAACTGC CCTCATAAAT 
AGTGACATAG CTGACCGGGA GCAGAGGTTA AAAGGCTTGG AACAAGTTAC TAAGGCTACT 
ATGCTCGGTC ACCTTCTTCC TGTGTTACTG ACCTCCCTGA TGCATCCAAA TTTACAGACT 
CTGACCATGG CCGATGCCCT GATGCCTCAG CTAGTGCAGC TGGTACTTTA TACCAGTCAG 
ACGGCTTTGC TGCTCAAAAC CCAGTGTCCG GTTTTTGCTG AGGCGGGCTG TTCCCCATGT 
GGTGCATCAG ACCAGAAGGG CAGGCTGTTC CCTGATGAGA GAATGCTGGA AGAGAAGGAA 
GAGCCAGGGT TTCTCACTGG TTTAAAGATT CCTGCTCCAT GGGCTGCTGG GAAGACTGTG 
GAAACAGTCC ATCCTGTCAG AGACAACTAT AAATTTAAAG AAACTGTCCA TATCCCGGGA 
GCTCGCTGCC TATACCTTAG ATTTGATAGC AGATGCTCTT CACAATATGA CTATGACAAA 
TCTAACAGTA TATTTTCTGT TTTGAAGTTG GTGATATACG CGGGGCCTAA CACAAACAGT 
AGGAAGGTTG CTGAGTATGG AGGCAACACT CTGGGATATG GCAGCCGTAG TGTCTTAGGA 
ACTGGTTGGC CGAAAGACTT GGTGAAGGTG GAAGGAGATA CAGTCACCTT CTCCTTTGAA 
ATGAGAAGTG GCCGTGAACA CAACACTCCC GATAAAGCTA TGTGGGGCTT TGCTTGCACA 
GTTCGCGCTC AGGAGTCTTC AGAGGATGTG TCGGGAGGCT TACCTTTTCT GGTAGACCTG 
GCTTTAGGTC TGTCTGTGTT AGCTTGTTCT ATGTTAAGAA TCCTATACAA CGGACCAGAA 
ATTACCAAAG AAGAAGAAGC CTGTCAGGAG CTGCTGCGGT CCAAACTTTT ACAAAGGTGC 
CAGTGGCAGG TGGAGGCCAA TGGTGTGATC TCCCCTGCCC TTACTCCAAG TCCGTCTCCA 
CTGCCTCTGA CCATCGAGGA AGACAGAGAA TTCACTTACC CCTCTGACGT CCTCGTGCCT 
CCTGTTGGAA ACTACTTTGA CCTGCCCCGG ATCAGGCTGC CTCCAGGAAT CATGATAAAG 
CTCAGGGAAA TTTCTGGGCG TGCCAGACCT CAATTTAGAC CAAGTATAAA AGAAGTAATC 
CAGCCAGACG TGATGGAGGA AATGGTGGTA TCGTGTGTCA TTAAGCACTT GAACTTGGTT 
GATGCACTGC AGTCCCTAAT AAATTTCCAG TATCAAGAAG AACATGCTGA GGAATATGAT 
TTATTGTGTA AAATCATGGG AGAGACATTT AAGAAACTCA ATGCCATGGA GAGACAGCTG 
CAGAGTGTTG CAGAATTGGA ACAGAAATGG CAAAGTGAGG TTGATGATGC CACGCAGGGG 
AAACTGGAGA ACAACATGCC TTTCTTCTAT GATTACCATT TTAATGAGAA CAAAATGAAA 
GAACTAGAAC TTTTGTGTTC AATGAAGGAA GTCTCCTTTG ATGGAAATGA TCTTGAAAAC 
ATGGTCCTAT CTCTGAGGGA GAAGTTCCTA CAAGAAGTGA ATTCTCTCAT TCAGAAACCC 
TCGCACCCAC TGGCTAAGAC GAAGACGTTA GTGAAGAGTC TAATGAACCG AGCTGAGCTG 
TTGCTGCACG TCACCATTGC GGCCCAGTCC GGCCTCACAA GAAGCATCTC AGGGACCCCC 
GCAGAAACAC CGGCTTGTAA ATCAGCCTCT GAAACAAAGG TGATATCTCA TGCTGTGAGG 
CAGCCTGTTT TTCTTCGCAG CATGTCGGCT CCTTCTGACC TGGAAATGAT TGGTAACGAT 
GATTTGGAAT TTACTAGAGC AAATCAGAGG CGGCGCCACG TGACCAGCCA CCGCAGCAGC 
TCCTTCACGC TCCTGCAGTC ATTGGCCATC GAGGACAGCA GGGACAAGCC CACCTACAGC 
GTCCTGCTGG GGCAGCTGTT TGCTTTCATC GGCACCAACC CTGACCAAGC TGTGTCCAGC 
AGCAGCTTTC TTTTGGCTGC GCAGACAAGG TGGCGACGGG GAAACACTCG CAAGCAGGCA 
CTGGTGCACA TGCGGGAATT GCTGACGGCT GCCGTGCGAG TCGGGGGAGT GACACATCTT 
GTGGGTCCGG TGACTATGGT CCTTCAGGGA GGACCCAGAA TTGAAGAACT AACCTGTGGT 
GGGATGGTCG AGCAGGTCCA GGAAGCCTTC GGTGAGACCA TGACCTCTGT TGTGTCTTTG 
TGCGCCCGTT ACCCTATTGC TTGTGCGAAT AGCATCGGAC TCTTATGTAC TATACCTTAC 
ACCAGGAGTG AAGAGAAGTG CCTGGTGCGC AGTGGCCTTG TTCAGCTCAT GGATCGACTG 
TGTAGCTTGA GCAGTCAGAC TGAGTCCAGC TCCAGTGAGA AACAAACCAA GAAGCAGAAG 
GTGGCTACCA TGGCCTGGGC AGCCTTCCAG GTGCTTGCCA ACCGCTGCGT TGAATGGGAA 
AAAGAAGAAG GCGGCTCCAC AGAGGCTGTG CACTCAGGTC TGGCCCGTCA GGTGTCCAGC 
CTTCTCACTA ACCACCTCGC CCGAGCGACG GAGAGCTGCG GCAACCAGGC TGCCGGGAAC 
GATGCCCTCC AGGACGTCCT GAGTCTTCTC AATGACCTCT CGAGGAGTCA CATAGGTAAA 
GCCATCCTGA GCCAGCCAGC TTGTGTGTCC AAACTCCTCT CCCTGCTGCT TGACCAACGC 
CCGTCCCCAA AGCTGGTCCT TATCATTCTT CAGCTGTGCC GGGCAGCACT CCCGCTGATG 
AGCGTAGAAG ACTGTGGGAA TGTGGAGCTC CCACCCTGGA GCTACTCTGT CCCCTCCCTA 
AACAGTGAGC AAGAGGATCC CAGCGACCCA GCGTCCAAGA TCGCCTCTTT GCTCTTAGCA 
AAGCTAGCAG ATTATGTGGT CCCAGGATGT CAGACAGTCC TTTCTCCAAC CGCTTCTGAA 
CCTGACACCA TGTTGACAAA AACCAGCCCC AAGAGTTCCT TAAAAGGAGA TAAAGACCCT 
GGAGAAGAGA GTGAAGCAGT GGATGGCAAG CTCTCTATCT TTATCCACAA GCGGGAAGAC 
CAGTCATCCC ATGAAGTTCT CCAACCATTA CTAAGTAGCT CAGAAGGACG GCCCTTCCGA 
CTTGGTACTG GTGCCAACAT GGAGAAAGTC GTGAAAATGG ACCGAGACAT GACAAAGGGT 
GGCTGCTGTG AGGTGATTAC AGAAGAGGCG GCAGCGGCTC TGCGAAAAGC AACCAAGTGG 
GCACAGTCTG GCCTCATCGT CAGCGTCGGG CCACCCGTGG AATCGCTCAA CCCAGAGACT 
GTGAGTGGAC TGTCCACAGG GGACAAGAAG AAAACCGCCC AAACTTCCAT TTGCCGAGAG 
AGGAACTCTG AACTTGCCAG GACCGACCCT GTCCGACCTT TCATCAGTGG GCATGTGGCA 
AACAGCATGG CTGCAGAGGT GATCGCCTTG CTTCACAGCT TGCTGATGGC GCCTGAGTCA 
AATGCTGCTC AGATATGGAC CACGACAGCA GAGAAGGTTC TGTCTCGTGC GCTGATGTAC 
ATTCCACAGT TGGGGAAATA TGCAGAAAGC ATTCTGGAAA ATGGCAGCAG TAGTGGCAGA 
AAACTCGCCA AACTTCAGAG AATCGCCCGC CAGGCTGTCG CTGCACTGTG TGCACTTGGA 
GGCTTCAAAG AGACAATCAA GATAGGGTCT GAGGTTCAGG TTTTAGGTAG AGGAATATCA 
GGAAGTGTCG GAGTAGTGGC TTCTATCAAT GAACAGGAAG GTATAGCTAC AGTCAAATTC 
CCACCCATAG ACTGTAGAAA GACGTCGCAA GCATCAGACA CACTGACTAT TCCATTGTCT 
AGACTTTGTG TTCCAAGATC AGAGGCATTG CCTCTTCATA AACTGTCCAT TACTGAGAAG 
GTAGTACAGG CAGTCCAGTC CATGTTGCTT CCTCAGGAGG GAAGTCTCTC TATTCATACC 
TCACTTCCTG CAACAGGAGA TGGGTCAGCT CCTGTGATGG CAGTCGTTCG GCTCCTGGCT 
GAAATAAGAA CAAGAGCATG CCTGGTCATG GCCCAGCTTT TAGAAGACAG CTTATTTTGT 
GAAGAATTCA TACAGCAGTG TCCAGCAGCT GTTGAGGTTC TCAACCTAGT AGCCCAGGAA 
TGCAGTGCTG GAGAGCGACT GGCAGTTGTG GAAGTGCAGT GTGAACGGCT GAGGATGCTC 
TACCGGGACT GTGCTCGGCC CCCGCCACCT CCGCTGCAGG CTGACCGGAG ACAGCCCAAA 
GAGATTACGT GGAGCCCCTC ACGAGTGTTT CCACCTGTCC GAGCCTGCAT GTTCTCATCG 
CACCTCACTT CCGTCACCTT CCTGGCTGAC CCCAGCGCTG GGGGCGGTCT GCCCCGGGGC 
ACATTTATCT ATGCCACCTC ACCACTGCCC ATTCAGGCCC CATCTTTCTA CTGGGAAATT 
GAAATTGTTT CCTATGGAGA TAGTGATGAT GACACTGGAC CAATAGTTTC CTTTGGCTTT 
GCTACAGAAG CAGAGAAAAG AGACGGGGCT TGGACTAATC CAGTGGGCAC TTGTCTTTTC 
CATAACAATG GCCGAGCCGT GCACTACAAC GGCTCCAGTT TATTACAGTG GAAGAGCGTT 
CGCTTAGACG TGACTCTCTC TCCTGGTGAC GTGGCAGGAA TTGGCTGGGA GAGAACCGAG 
GGAACGCCTC CTCCTCCAGG GCAGCCAGCC AAGGGCCGAG TGTACTTCAC ATACTGCGGG 
CAGCGCCTCT CACCGTATCT TGAAGATGTT TCGGGTGGGA TGTGGCCTGT GGTTCACATT 
CAGAAAAAGA ACACCAAGAC GCGAGCTAAC TTTGGCTCCC GCCCATTTGC CTATGCTGAA 
GGGCAGGCCC ACCGCAACGC AGCTGACCTG TGCACCGACC TGGCAGAGGA GATCAGCGCT 
AACTTCGAGG CCCTGCCCTT TGCCATGGCA TCTGACAGTG ACAACGATGC TGGCACCAGT 
ATTGCGTCAG ACCCGGGCAC TCACGGGCCA CCGTGCCGGA TTGCTGCCGT GGCCACGGCT 
CAGCAACAAT ATGATAGTGA CACTTCCTGT CATTATAAAG TAGAGCTCAG CTATGAGAAT 
TTCATCACCT CCGGCCCAGA CCCCCATCCG CCCCCCATTG CAGATGACGA AAGTGATGAT 
GATGATGACG ATGATATCCC GCAGGAGGAC CACTATGCTC TACTGGTGAA AGCGTGGGAG 
ACCAAGGTTT TTCCTACTAT CCGAAGACGC TTCCGCAACG AAGCAGAGCG GAAATCGGGC 
CTGGACCAGA TAAAGGGTGC TTTACAACTA GGCATGGTGG ATATTGCCCG GCAGACAGTT 
GAATTCCTCT ACGAGGAGAA CGGTGGCATC CCCAGAGACC TTTATCTTCC CACCATTGAA 
GACATTAAGG ATGAAGCAAA CAAGTTCACA ATTGATAAAG TTCGAAAAGG TCTCACGGTA 
GTAACCCGCT CTCCAGACAG CAATAACGTA GCCAGCAGTA CTGTTGGAAC TGCCCTGCCA 
AAATTTGCCA TCCGAGGGAT GCTGAAAACC TTTGGGCTTC ATGGAGTTGT CTTAGATGTT 
GATTCAGTAA ATGAATTGGT GCAGGTAGAA ACGTACCTCC GAAGCGAAGG TGTGCTGGTC 
CGATACTGGT ATCCTATTGA TATGTTGGAA AGGCCCCCAG CAGGCTACCG AAGGACTGCC 
ACCAATGGGC TGGTCACCCT GGACAATACC AACCTTCAAA TTCACAGGGA GCTGCTGCGC 
TGCGAGGCCG CACTGGCCAG GCTGTACTGC CGCATGGCCC TGCTCAACAT CTTCGCCCCC 
AGGCTGCCTC ACCTGTTTAC CCGCCTCTTC CACATCCCTG CCATCCGGGA CATTACCCTG 
GAGCACCTGC AGCTGCTGTC CAATCAGCTC CTCGCTCCTC CCCTCCCAGA TGGCACCATC 
AGCTCCAGCT CCATCCTCCT GGCACAGTCG CTGCAGCACT GCATCCATTC CCAGAACTGC 
TCGGCCACGG ACCTCTTCTA CCAGGGCAGC TCCCAGACAG TGAGAGAGTG GCTCAACGTG 
GCCATCACTC GGACCCTGCA CCAGGGTGAG GAGAGCCTGT TGGAGCTGAC GAAACAGATC 
TGCTCTTTCC TGCAGACGGC ACCAGAGCAG TTCCCCTCCG AAGAGTTTCC AATTTCCGAG 
TCCAAGGTCA ACATGGACGT CAATTTCCCC GGGGCGGCTT TCGTCGTGGT ATCTTGCAAA 
GAAAGTCAGT CTGGATTCCG CAAAGACTCC TCTCTGTACA AGGCGCCGTG GGCGCGCGTG 
CTCGTGTACG GACTGGGCCA CAAAGTGAAG CGAAATGGCC AGCTAAACCT CATCGAGGCC 
ATCTGTTACC CGCGGGATGC GTCTCCGGCC AACACGGGGC TCACGCCGCC TCCCACCACC 
AACCAGTACC CTTCTGTGAT CCTCTCCACA GACAAAGTCC ACATCAAACT GGGGGTGTCT 
CCCCCTCCTG GAGCCGTGCT GGTGTTACAC TCTCTGCCCC TTGAGTTCCC ACTGGCGATG 
GCCTTCGCAG AGCAGCTGCT GTCCTGGAAG TCGGAGGACG GTGAAGGGAG ATCTGAAGAC 
GAGCCTGACA CCATCCCGAC GTCCGTCCTC CTGCAAGTGG TGGAACTGCT CGGAAACTTC 
CTGTGGACCA CGGACATGGC GGCCTGTGTG AAAGAGCTGG TTTTCCACCT CCTGGCAGAG 
CTCGTGCGCA CAGTGCACAG CCTGGAGCAG AGGAAGCACC CTGCCGGCCT GTCCTCGTCC 
ATCGCCCTCC AGCTGAACCC CTGCCTGGCC ATGCTGATGG CCCTGCAGTC AGAGCTCCAC 
AAGCTGTACG ACGAGGAGAC GCAGAGCTGG GTCTCGGGCA GCACGTGCGG AGGCTCGGGG 
ACCGTGGCGG CCACCGACCA GGGCAGGTTC TCTACGTATT TCCACGCGCT CATGGAAGGG 
TGCCTGGCGG TGGCCGAGGT GACCCTGCCT ACCAACATGA GCGTCACTGC CAGCGGGGTG 
ACGTCGGCGA CCGCCCCAAA CCTCAGTGAC TCGTCGTCCT CCTCGTCATC CTCCCCAGGA 
CAGACGCCGC AGAGCCCCAG CCTCCTGTCC AAGAGGAAGA AAGTCAAGAT GAAGCGGGAA 
AAGGCCTCCT CCTCTGGGAA GCGCCAGTCC TCGCACTCAG TGGACTCGGA CCCCGCCGTG 
CTGAGCATCG GGGGTGGCAA GCCCGAAGAC ATGCTGTGGT TCCATCGCGC CCTGACCTTG 
CTCATCATCC TCCGGCACCT CACCAGGAAG GACCCGCAGG GGCTGGGTGT GACGAGCGAC 
GCCATCGCCG ACGCCTGCCA GGCCCTGGTG GGTCCCACCG CCCACAGCCG CTTGCTGGTG 
ATCTCTGGGA TCCCCACCCA CCTGGACGAG GGCATCGTCA GAGGCGCCAT CCGCAAGGCC 
TGCAACGCCC ACGGCGGGGT CTTCAAAGAC GAGATCTACA TCCCCCTGCA GGACGAAGAC 
CCCAAGAAGC CAAAAGACAA GGCCGAGGGC GGTGATGGGA AAGCCGAGCC AGAGAAGACC 
CTTGGCTTCC CCAGCACAGA CAGCTTGGAG GTCAGCACGT CCCGCAGCCC GACCCCCGCC 
ATGAGCATCA GCGCGTCTGC GTCCACCAGC CAAGCCTCCA TCTGCAGCTC CCAGGGCATC 
TTGCAGACAG TCAGCGATGT CTCTGTGGAA CCGCTGCCCT CGGGCCTCGA GCTGCCCATC 
CCTGCGGGCT TATTGGAGCC CCACGTAGTG TCCAGCCAGG AGAGCCTGGA CGTTTCCCTG 
TGCAGCACCA GCAGCCTGGG CAGCCTCGGG AGCCTGGGGG AGCCACTGGA CAACGCAGAA 
ACGGCCTCAG TGTCAGACAT GGGCTCCATG TACACAGTCA CGTCCCTGGA CAACCAGCCC 
CTCGCAGCAC GCCCCATCAA AGGCTTCGCG GTCGTGGAGA TTAGATCCCG AGCCAAAATC 
GAGAAAATCC GAGCGAGTTT ATTTAACAAT AATGACTTGA TTGGTTTGTC AAGTTTGGAT 
GGTGAAGATG AATTGATGGA AATGTCAACG GAAGAGATCC TCACTGTGTC TGTAGTAAAT 
CAGAGTTTGT TTGACACGCA GGGGAGCCCA GGGTTAGAAG ATTATTTCAA TGATAAGTCA 
ATTAAAGGTG AGAAGCTGGT GCCCGGGGCC AGAGAGGTGC TGACGGAGAT ATTTAAGAGC 
TGCGCCCACT CAGAGCAGAT GCTGAGTCTG ACACCAGCAA AGCCCATCAA AGTGTCTGAC 
ATTTATCTCA GCAAAGAGCA GATCAACTCC CAGACGCCAG GCAACCTCCT CCACCTCTTC 
TTCACCAATG TTCGGCCCCC CAAAAAGGTG CTGGAGGACC AGCTCACCCA GATCCTGAGG 
AAGTACGGCG TGCCAAAACC CAAGTTCGAC AAGAGCAAGT ACAGCAAGGC GGGGAAGGAA 
CAGCACCCCG GGAAGGTGGT GAGCACCAAG CGGCCCATCA CCAAGCCACC TGCCAAGGAC 
AAGGCCGTGC TCAACAGCGT CAGCAGGACT GCCTTGAGCG AGAAGAAGCC CACTGTGAAG 
CCGAAGTCAC CGGAAAAGAG CAAGCCGGAC GAAAAGGACC CGGAAAAGTC ACCCACCAAA 
AAACAAGAAG TCCCTGAGGA AAAGTACCTG ACATTGGAAG GATTTCACAA GTTTGTTATT 
GACCGAGCCA AGCAAGACAT CCGTAGTGTC TGGAGGGCGA TTTTATCCTG TGGTTACGAT 
CTTCATTTTG AGAGGTGTGC CTGCATCGAC GTCCGGCACG CACACAAGGC CTCCAGGAAG 
TGGACCCTGG AGATGGACGT GGCGCTCGTG CAGTACATCA ACCGGCTGTG CCGCCACCTG 
GCCATCACGC CCGCGCGGCT GCATCCCCAC GAGGTGTATC TGGAGACCAC AGACGCCGCC 
GACCCCAGAG TGGCCTGTCT CTTGAATGTG CCCATTGAGA GCCTGCGCCT GCGCTTCGCC 
CTGCTGCAGT CCCTGAACAC CACGCTCGAG ACCTTCTTCC TGCCACTCGT GGAGCTGCGC 
CAGACGCCCA TGTACACGCA CAGCATCGCG GCCCTGCTGA AGGAGGCCAA AGGGCTGATC 
TTTTATGACA CGAAGGTGAC CGTCATGAAT CGGGTGCTGA ACGCCACAGT GCAGAGGACG 
GCTGACCACG CGGCACCGGA GATTACTCTG GACCCACTGG AGATCGTGGG AGGGGAGATC 
AGAGCCTCTG AAAACTCCTA CTTCTGCCAG GCGGCCAGGC AGCTGGCCTC GGTGCCATCC 
TCCCAGCTTT GCGTCAGACT GGCGAGTGGC GGTGACCCCA CCTATGCCTT CAACATCCGC 
TTCACTGGGG AGGAGGTCCA CGGCACCAGC GGCTCCTTCC GCCACTTCCT GTGGCAGGTG 
TGTAAAGAGC TGCAGAGCTC CGCGCTCTCC CTTCTCCTGC TGTGCCCCAG CTCAGCCGTC 
AACAAGAACA AGGGCCAGTA CATCCTGAGC CCAAGCCCCA TCAGCTACGG GGAGGAGCAG 
CTGCTGCACT TCCTGGGCCA GTTGCTGGGA ATTGCGATTC GAGCAGATGT CCCTCTTCCC 
CTGGACCTCC TACCCTCCTT CTGGAAGACG CTGGTGGGCG AGCCCCTGGA CCCTGACCAA 
GACCTGCAGG AAGCAGACAT ACTCACCTAC AATTATGTCA AGAAATTCGA GAGTATCAAC 
GACGAGAGCG AGCTGGAGGC CCTGTGCGCC GAGATTGCCT CCCAGCACCT GGCCATCGAG 
AGCCCCGACG GGCCCAGTAA GCCCTGCTGC CGGTTCACCT ACCTGACCAT GGCGGGCGAG 
GAGGTGGAGC TGTGCAGCCG CGGCCGGCAC ATCCCTGTGG CGTGGGAGAA CAAGGACATC 
TACGCAGCAG CCATCCGGAG CCTGCGGCTG CGGGAGCTGC AGAACGTCGA GTGTGTGACA 
GCCGTGCGCG CGGGCCTCGG CGCCATCATC CCCCTGCAGC TGCTCACCAC GCTCAGCCCG 
CTGGAGATGG AGCTGCGGAC GTGCGGCCTC CCTTACATCA ACCTCGAGTT CCTGAAGGCC 
CACACCATGT ACCAGGTGGG CCTGATGGAG ACGGACCAGC ACATCGAGTT CTTCTGGGGG 
GCACTGGAGA TGTTTGCCCA GGAGGAGCTG TGCAAGTTCA TCAAGTTTGC CTGCAACCAA 
GAGCGCATCC CGTTCACCTG CCCCTGCAAA GACGGGGGCC CCGACACAGC CCACGTGCCC 
CCATACCCCA TGAAGATCGC CCCCCCGGAT GGCACAGCAG GTTCCCCAGA CTCTCGCTAC 
ATCCGAGTGG AGACCTGCAT GTTCATGATC AAGCTTCCCC AGTACTCCTC TCTGGAAATC 
ATGCTGGAGA AACTTCGTTG TGCCATTCAC TATCGCGAAG ACCCCCTGAG TGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD4 ( XM_007114336.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007114398.1 Physeter catodon HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X2, mRNA.

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CloneID	OPh122638
Clone ID Related Accession (Same CDS sequence)	XM_007114336.1
Accession Version	XM_007114336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1394380800000
Organism	Physeter catodon(sperm whale)
Product	probable E3 ubiquitin-protein ligase HECTD4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006714665.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Physeter catodon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAAG AGGGCGAGTC TGCAAAAAGC ATTAATGAGA TGCTTCTGAG CAGACTTTCA 
CGCTACCGAG CCTCGCCATC CGCAACGCTG GCTGCCCTTA CCGGCTCCAC CATCTCCAAC 
ACCCTGAAAG AGGACCAGGC AGCAAATACT AGCTGTGGGC TTCCTCTAAA AATGCTTCGG 
AAGACGCCCA TTTACACCTG TGGTACATAC TTGGTGATGC TGGTCCCACC TCCAGGGGGA 
TCAGGCAGCT CTGCTACCCG GTCTCTTTTT GGTGGAACAA GTGGTTTGTC GTCTTTAAAA 
ATCCTAGCTT CTAGCTTGGT GTATAATATT TCGGATGGTC AATTCGCAAG TCGTGCTGAT 
CTCATAGATG CTGCTGGAAG CTCATTGGGG CGTGGTGCTC TTGTACCAGG ATTAGGTGCC 
TGTTACGATA CAGTGAACAA CATGCTATGG ACATGCTCGA ATGACTATAT CGATCAATGG 
TGTAATCCTG GGAATCAGGC CTTCCATTAC GTCTGTCAGA GACTTGGAGT CAGCCATATC 
ATCACTGAGC CTAAAGAAGA GGCTATAACC ACGAATGAGG TTATAAACCA ATTATTGCAC 
CACGTTGGTG CGATGTGCAT ACACCAACTC AATCTTCTTG CTACCAACCC CAATCTTCCA 
ATCACAAGTG TCTTGGGCAA GCAGCATCCA ATTGAAGCAC ACCATCTTAG CAGTATTTGT 
GACATTATGG AGAAGGCCAT GGTTAATGGA GACACCTGTA TCATACGCTG CATTCTCGTT 
GTCTTTCAGG TGGTGTTTAA ATTTTTCTTC AGCCCACAAA CTGAAAGGAA TCGAGACATC 
ATTCGACGGT CGGGGTTGCT TCTTTGGCAG TTGTTGATGG CACCAAAAGA TCAAATTTGT 
CCTGAAATTC AGAAGGAAGT CTGCCTTGCC ATCAGCTCTG GTTTAAATAT CTTGTACCCA 
GGTGAAACTG AAATCAATAA CTTACTTAAG CTGGTCTTAA CAGAAGGAGA GAGAAACAGT 
GGCCTCTCCC AGCTGCGGGA TGTGATCCTA ACCAATTTAG CTGAACAGCT TCAAAACAAC 
CGATTTGGCA GTGAGGAGGA TGATCATTAC AGACTAAATG ACGAACTTTT ACACTACATT 
TTGAAGATTG TTGTACGAGA ATCCTGTATC TTAATCACCA AGTGCCAAAC TGTCTCTAAA 
GATGATTTTC AAAAACTCCT TTCAACTGTG CCTGCTGCAT CCTCCTGCCT GCGCTATCTG 
ATGGCAGTTC AGAACCACCT TCTCAGTAAC ACTGTTTTGA TTAAACCTGA TGAGAATGAT 
GACAGTGACA ACTCCTTGCA GGGAGAGACA TTGAAGGAGC TAAAGGTCAG TATTTTGGCT 
CTTGCCACCC AAATCCTGAC TGGATGTGAT GAAGTGTTGG AAATGCTACA GCAGGTCACA 
ACTGCCCTCA TAAATAGTGA CATAGCTGAC CGGGAGCAGA GGTTAAAAGG CTTGGAACAA 
GTTACTAAGG CTACTATGCT CGGTCACCTT CTTCCTGTGT TACTGACCTC CCTGATGCAT 
CCAAATTTAC AGACTCTGAC CATGGCCGAT GCCCTGATGC CTCAGCTAGT GCAGCTGGTA 
CTTTATACCA GTCAGACGGC TTTGCTGCTC AAAACCCAGT GTCCGGTTTT TGCTGAGGCG 
GGCTGTTCCC CATGTGGTGC ATCAGACCAG AAGGGCAGGC TGTTCCCTGA TGAGAGAATG 
CTGGAAGAGA AGGAAGAGCC AGGGTTTCTC ACTGGTTTAA AGATTCCTGC TCCATGGGCT 
GCTGGGAAGA CTGTGGAAAC AGTCCATCCT GTCAGAGACA ACTATAAATT TAAAGAAACT 
GTCCATATCC CGGGAGCTCG CTGCCTATAC CTTAGATTTG ATAGCAGATG CTCTTCACAA 
TATGACTATG ACAAATTGGT GATATACGCG GGGCCTAACA CAAACAGTAG GAAGGTTGCT 
GAGTATGGAG GCAACACTCT GGGATATGGC AGCCGTAGTG TCTTAGGAAC TGGTTGGCCG 
AAAGACTTGG TGAAGGTGGA AGGAGATACA GTCACCTTCT CCTTTGAAAT GAGAAGTGGC 
CGTGAACACA ACACTCCCGA TAAAGCTATG TGGGGCTTTG CTTGCACAGT TCGCGCTCAG 
GAGTCTTCAG AGGATGTGTC GGGAGGCTTA CCTTTTCTGG TAGACCTGGC TTTAGGTCTG 
TCTGTGTTAG CTTGTTCTAT GTTAAGAATC CTATACAACG GACCAGAAAT TACCAAAGAA 
GAAGAAGCCT GTCAGGAGCT GCTGCGGTCC AAACTTTTAC AAAGGTGCCA GTGGCAGGTG 
GAGGCCAATG GTGTGATCTC CCCTGCCCTT ACTCCAAGTC CGTCTCCACT GCCTCTGACC 
ATCGAGGAAG ACAGAGAATT CACTTACCCC TCTGACGTCC TCGTGCCTCC TGTTGGAAAC 
TACTTTGACC TGCCCCGGAT CAGGCTGCCT CCAGGAATCA TGATAAAGCT CAGGGAAATT 
TCTGGGCGTG CCAGACCTCA ATTTAGACCA AGTATAAAAG AAGTAATCCA GCCAGACGTG 
ATGGAGGAAA TGGTGGTATC GTGTGTCATT AAGCACTTGA ACTTGGTTGA TGCACTGCAG 
TCCCTAATAA ATTTCCAGTA TCAAGAAGAA CATGCTGAGG AATATGATTT ATTGTGTAAA 
ATCATGGGAG AGACATTTAA GAAACTCAAT GCCATGGAGA GACAGCTGCA GAGTGTTGCA 
GAATTGGAAC AGAAATGGCA AAGTGAGGTT GATGATGCCA CGCAGGGGAA ACTGGAGAAC 
AACATGCCTT TCTTCTATGA TTACCATTTT AATGAGAACA AAATGAAAGA ACTAGAACTT 
TTGTGTTCAA TGAAGGAAGT CTCCTTTGAT GGAAATGATC TTGAAAACAT GGTCCTATCT 
CTGAGGGAGA AGTTCCTACA AGAAGTGAAT TCTCTCATTC AGAAACCCTC GCACCCACTG 
GCTAAGACGA AGACGTTAGT GAAGAGTCTA ATGAACCGAG CTGAGCTGTT GCTGCACGTC 
ACCATTGCGG CCCAGTCCGG CCTCACAAGA AGCATCTCAG GGACCCCCGC AGAAACACCG 
GCTTGTAAAT CAGCCTCTGA AACAAAGGTG ATATCTCATG CTGTGAGGCA GCCTGTTTTT 
CTTCGCAGCA TGTCGGCTCC TTCTGACCTG GAAATGATTG GTAACGATGA TTTGGAATTT 
ACTAGAGCAA ATCAGAGGCG GCGCCACGTG ACCAGCCACC GCAGCAGCTC CTTCACGCTC 
CTGCAGTCAT TGGCCATCGA GGACAGCAGG GACAAGCCCA CCTACAGCGT CCTGCTGGGG 
CAGCTGTTTG CTTTCATCGG CACCAACCCT GACCAAGCTG TGTCCAGCAG CAGCTTTCTT 
TTGGCTGCGC AGACAAGGTG GCGACGGGGA AACACTCGCA AGCAGGCACT GGTGCACATG 
CGGGAATTGC TGACGGCTGC CGTGCGAGTC GGGGGAGTGA CACATCTTGT GGGTCCGGTG 
ACTATGGTCC TTCAGGGAGG ACCCAGAATT GAAGAACTAA CCTGTGGTGG GATGGTCGAG 
CAGGTCCAGG AAGCCTTCGG TGAGACCATG ACCTCTGTTG TGTCTTTGTG CGCCCGTTAC 
CCTATTGCTT GTGCGAATAG CATCGGACTC TTATGTACTA TACCTTACAC CAGGAGTGAA 
GAGAAGTGCC TGGTGCGCAG TGGCCTTGTT CAGCTCATGG ATCGACTGTG TAGCTTGAGC 
AGTCAGACTG AGTCCAGCTC CAGTGAGAAA CAAACCAAGA AGCAGAAGGT GGCTACCATG 
GCCTGGGCAG CCTTCCAGGT GCTTGCCAAC CGCTGCGTTG AATGGGAAAA AGAAGAAGGC 
GGCTCCACAG AGGCTGTGCA CTCAGGTCTG GCCCGTCAGG TGTCCAGCCT TCTCACTAAC 
CACCTCGCCC GAGCGACGGA GAGCTGCGGC AACCAGGCTG CCGGGAACGA TGCCCTCCAG 
GACGTCCTGA GTCTTCTCAA TGACCTCTCG AGGAGTCACA TAGGTAAAGC CATCCTGAGC 
CAGCCAGCTT GTGTGTCCAA ACTCCTCTCC CTGCTGCTTG ACCAACGCCC GTCCCCAAAG 
CTGGTCCTTA TCATTCTTCA GCTGTGCCGG GCAGCACTCC CGCTGATGAG CGTAGAAGAC 
TGTGGGAATG TGGAGCTCCC ACCCTGGAGC TACTCTGTCC CCTCCCTAAA CAGTGAGCAA 
GAGGATCCCA GCGACCCAGC GTCCAAGATC GCCTCTTTGC TCTTAGCAAA GCTAGCAGAT 
TATGTGGTCC CAGGATGTCA GACAGTCCTT TCTCCAACCG CTTCTGAACC TGACACCATG 
TTGACAAAAA CCAGCCCCAA GAGTTCCTTA AAAGGAGATA AAGACCCTGG AGAAGAGAGT 
GAAGCAGTGG ATGGCAAGCT CTCTATCTTT ATCCACAAGC GGGAAGACCA GTCATCCCAT 
GAAGTTCTCC AACCATTACT AAGTAGCTCA GAAGGACGGC CCTTCCGACT TGGTACTGGT 
GCCAACATGG AGAAAGTCGT GAAAATGGAC CGAGACATGA CAAAGGGTGG CTGCTGTGAG 
GTGATTACAG AAGAGGCGGC AGCGGCTCTG CGAAAAGCAA CCAAGTGGGC ACAGTCTGGC 
CTCATCGTCA GCGTCGGGCC ACCCGTGGAA TCGCTCAACC CAGAGACTGT GAGTGGACTG 
TCCACAGGGG ACAAGAAGAA AACCGCCCAA ACTTCCATTT GCCGAGAGAG GAACTCTGAA 
CTTGCCAGGA CCGACCCTGT CCGACCTTTC ATCAGTGGGC ATGTGGCAAA CAGCATGGCT 
GCAGAGGTGA TCGCCTTGCT TCACAGCTTG CTGATGGCGC CTGAGTCAAA TGCTGCTCAG 
ATATGGACCA CGACAGCAGA GAAGGTTCTG TCTCGTGCGC TGATGTACAT TCCACAGTTG 
GGGAAATATG CAGAAAGCAT TCTGGAAAAT GGCAGCAGTA GTGGCAGAAA ACTCGCCAAA 
CTTCAGAGAA TCGCCCGCCA GGCTGTCGCT GCACTGTGTG CACTTGGAGG CTTCAAAGAG 
ACAATCAAGA TAGGGTCTGA GGTTCAGGTT TTAGGTAGAG GAATATCAGG AAGTGTCGGA 
GTAGTGGCTT CTATCAATGA ACAGGAAGGT ATAGCTACAG TCAAATTCCC ACCCATAGAC 
TGTAGAAAGA CGTCGCAAGC ATCAGACACA CTGACTATTC CATTGTCTAG ACTTTGTGTT 
CCAAGATCAG AGGCATTGCC TCTTCATAAA CTGTCCATTA CTGAGAAGGT AGTACAGGCA 
GTCCAGTCCA TGTTGCTTCC TCAGGAGGGA AGTCTCTCTA TTCATACCTC ACTTCCTGCA 
ACAGGAGATG GGTCAGCTCC TGTGATGGCA GTCGTTCGGC TCCTGGCTGA AATAAGAACA 
AGAGCATGCC TGGTCATGGC CCAGCTTTTA GAAGACAGCT TATTTTGTGA AGAATTCATA 
CAGCAGTGTC CAGCAGCTGT TGAGGTTCTC AACCTAGTAG CCCAGGAATG CAGTGCTGGA 
GAGCGACTGG CAGTTGTGGA AGTGCAGTGT GAACGGCTGA GGATGCTCTA CCGGGACTGT 
GCTCGGCCCC CGCCACCTCC GCTGCAGGCT GACCGGAGAC AGCCCAAAGA GATTACGTGG 
AGCCCCTCAC GAGTGTTTCC ACCTGTCCGA GCCTGCATGT TCTCATCGCA CCTCACTTCC 
GTCACCTTCC TGGCTGACCC CAGCGCTGGG GGCGGTCTGC CCCGGGGCAC ATTTATCTAT 
GCCACCTCAC CACTGCCCAT TCAGGCCCCA TCTTTCTACT GGGAAATTGA AATTGTTTCC 
TATGGAGATA GTGATGATGA CACTGGACCA ATAGTTTCCT TTGGCTTTGC TACAGAAGCA 
GAGAAAAGAG ACGGGGCTTG GACTAATCCA GTGGGCACTT GTCTTTTCCA TAACAATGGC 
CGAGCCGTGC ACTACAACGG CTCCAGTTTA TTACAGTGGA AGAGCGTTCG CTTAGACGTG 
ACTCTCTCTC CTGGTGACGT GGCAGGAATT GGCTGGGAGA GAACCGAGGG AACGCCTCCT 
CCTCCAGGGC AGCCAGCCAA GGGCCGAGTG TACTTCACAT ACTGCGGGCA GCGCCTCTCA 
CCGTATCTTG AAGATGTTTC GGGTGGGATG TGGCCTGTGG TTCACATTCA GAAAAAGAAC 
ACCAAGACGC GAGCTAACTT TGGCTCCCGC CCATTTGCCT ATGCTGAAGG GCAGGCCCAC 
CGCAACGCAG CTGACCTGTG CACCGACCTG GCAGAGGAGA TCAGCGCTAA CTTCGAGGCC 
CTGCCCTTTG CCATGGCATC TGACAGTGAC AACGATGCTG GCACCAGTAT TGCGTCAGAC 
CCGGGCACTC ACGGGCCACC GTGCCGGATT GCTGCCGTGG CCACGGCTCA GCAACAATAT 
GATAGTGACA CTTCCTGTCA TTATAAAGTA GAGCTCAGCT ATGAGAATTT CATCACCTCC 
GGCCCAGACC CCCATCCGCC CCCCATTGCA GATGACGAAA GTGATGATGA TGATGACGAT 
GATATCCCGC AGGAGGACCA CTATGCTCTA CTGGTGAAAG CGTGGGAGAC CAAGGTTTTT 
CCTACTATCC GAAGACGCTT CCGCAACGAA GCAGAGCGGA AATCGGGCCT GGACCAGATA 
AAGGGTGCTT TACAACTAGG CATGGTGGAT ATTGCCCGGC AGACAGTTGA ATTCCTCTAC 
GAGGAGAACG GTGGCATCCC CAGAGACCTT TATCTTCCCA CCATTGAAGA CATTAAGGAT 
GAAGCAAACA AGTTCACAAT TGATAAAGTT CGAAAAGGTC TCACGGTAGT AACCCGCTCT 
CCAGACAGCA ATAACGTAGC CAGCAGTACT GTTGGAACTG CCCTGCCAAA ATTTGCCATC 
CGAGGGATGC TGAAAACCTT TGGGCTTCAT GGAGTTGTCT TAGATGTTGA TTCAGTAAAT 
GAATTGGTGC AGGTAGAAAC GTACCTCCGA AGCGAAGGTG TGCTGGTCCG ATACTGGTAT 
CCTATTGATA TGTTGGAAAG GCCCCCAGCA GGCTACCGAA GGACTGCCAC CAATGGGCTG 
GTCACCCTGG ACAATACCAA CCTTCAAATT CACAGGGAGC TGCTGCGCTG CGAGGCCGCA 
CTGGCCAGGC TGTACTGCCG CATGGCCCTG CTCAACATCT TCGCCCCCAG GCTGCCTCAC 
CTGTTTACCC GCCTCTTCCA CATCCCTGCC ATCCGGGACA TTACCCTGGA GCACCTGCAG 
CTGCTGTCCA ATCAGCTCCT CGCTCCTCCC CTCCCAGATG GCACCATCAG CTCCAGCTCC 
ATCCTCCTGG CACAGTCGCT GCAGCACTGC ATCCATTCCC AGAACTGCTC GGCCACGGAC 
CTCTTCTACC AGGGCAGCTC CCAGACAGTG AGAGAGTGGC TCAACGTGGC CATCACTCGG 
ACCCTGCACC AGGGTGAGGA GAGCCTGTTG GAGCTGACGA AACAGATCTG CTCTTTCCTG 
CAGACGGCAC CAGAGCAGTT CCCCTCCGAA GAGTTTCCAA TTTCCGAGTC CAAGGTCAAC 
ATGGACGTCA ATTTCCCCGG GGCGGCTTTC GTCGTGGTAT CTTGCAAAGA AAGTCAGTCT 
GGATTCCGCA AAGACTCCTC TCTGTACAAG GCGCCGTGGG CGCGCGTGCT CGTGTACGGA 
CTGGGCCACA AAGTGAAGCG AAATGGCCAG CTAAACCTCA TCGAGGCCAT CTGTTACCCG 
CGGGATGCGT CTCCGGCCAA CACGGGGCTC ACGCCGCCTC CCACCACCAA CCAGTACCCT 
TCTGTGATCC TCTCCACAGA CAAAGTCCAC ATCAAACTGG GGGTGTCTCC CCCTCCTGGA 
GCCGTGCTGG TGTTACACTC TCTGCCCCTT GAGTTCCCAC TGGCGATGGC CTTCGCAGAG 
CAGCTGCTGT CCTGGAAGTC GGAGGACGGT GAAGGGAGAT CTGAAGACGA GCCTGACACC 
ATCCCGACGT CCGTCCTCCT GCAAGTGGTG GAACTGCTCG GAAACTTCCT GTGGACCACG 
GACATGGCGG CCTGTGTGAA AGAGCTGGTT TTCCACCTCC TGGCAGAGCT CGTGCGCACA 
GTGCACAGCC TGGAGCAGAG GAAGCACCCT GCCGGCCTGT CCTCGTCCAT CGCCCTCCAG 
CTGAACCCCT GCCTGGCCAT GCTGATGGCC CTGCAGTCAG AGCTCCACAA GCTGTACGAC 
GAGGAGACGC AGAGCTGGGT CTCGGGCAGC ACGTGCGGAG GCTCGGGGAC CGTGGCGGCC 
ACCGACCAGG GCAGGTTCTC TACGTATTTC CACGCGCTCA TGGAAGGGTG CCTGGCGGTG 
GCCGAGGTGA CCCTGCCTAC CAACATGAGC GTCACTGCCA GCGGGGTGAC GTCGGCGACC 
GCCCCAAACC TCAGTGACTC GTCGTCCTCC TCGTCATCCT CCCCAGGACA GACGCCGCAG 
AGCCCCAGCC TCCTGTCCAA GAGGAAGAAA GTCAAGATGA AGCGGGAAAA GGCCTCCTCC 
TCTGGGAAGC GCCAGTCCTC GCACTCAGTG GACTCGGACC CCGCCGTGCT GAGCATCGGG 
GGTGGCAAGC CCGAAGACAT GCTGTGGTTC CATCGCGCCC TGACCTTGCT CATCATCCTC 
CGGCACCTCA CCAGGAAGGA CCCGCAGGGG CTGGGTGTGA CGAGCGACGC CATCGCCGAC 
GCCTGCCAGG CCCTGGTGGG TCCCACCGCC CACAGCCGCT TGCTGGTGAT CTCTGGGATC 
CCCACCCACC TGGACGAGGG CATCGTCAGA GGCGCCATCC GCAAGGCCTG CAACGCCCAC 
GGCGGGGTCT TCAAAGACGA GATCTACATC CCCCTGCAGG ACGAAGACCC CAAGAAGCCA 
AAAGACAAGG CCGAGGGCGG TGATGGGAAA GCCGAGCCAG AGAAGACCCT TGGCTTCCCC 
AGCACAGACA GCTTGGAGGT CAGCACGTCC CGCAGCCCGA CCCCCGCCAT GAGCATCAGC 
GCGTCTGCGT CCACCAGCCA AGCCTCCATC TGCAGCTCCC AGGGCATCTT GCAGACAGTC 
AGCGATGTCT CTGTGGAACC GCTGCCCTCG GGCCTCGAGC TGCCCATCCC TGCGGGCTTA 
TTGGAGCCCC ACGTAGTGTC CAGCCAGGAG AGCCTGGACG TTTCCCTGTG CAGCACCAGC 
AGCCTGGGCA GCCTCGGGAG CCTGGGGGAG CCACTGGACA ACGCAGAAAC GGCCTCAGTG 
TCAGACATGG GCTCCATGTA CACAGTCACG TCCCTGGACA ACCAGCCCCT CGCAGCACGC 
CCCATCAAAG GCTTCGCGGT CGTGGAGATT AGATCCCGAG CCAAAATCGA GAAAATCCGA 
GCGAGTTTAT TTAACAATAA TGACTTGATT GGTTTGTCAA GTTTGGATGG TGAAGATGAA 
TTGATGGAAA TGTCAACGGA AGAGATCCTC ACTGTGTCTG TAGTAAATCA GAGTTTGTTT 
GACACGCAGG GGAGCCCAGG GTTAGAAGAT TATTTCAATG ATAAGTCAAT TAAAGGTGAG 
AAGCTGGTGC CCGGGGCCAG AGAGGTGCTG ACGGAGATAT TTAAGAGCTG CGCCCACTCA 
GAGCAGATGC TGAGTCTGAC ACCAGCAAAG CCCATCAAAG TGTCTGACAT TTATCTCAGC 
AAAGAGCAGA TCAACTCCCA GACGCCAGGC AACCTCCTCC ACCTCTTCTT CACCAATGTT 
CGGCCCCCCA AAAAGGTGCT GGAGGACCAG CTCACCCAGA TCCTGAGGAA GTACGGCGTG 
CCAAAACCCA AGTTCGACAA GAGCAAGTAC AGCAAGGCGG GGAAGGAACA GCACCCCGGG 
AAGGTGGTGA GCACCAAGCG GCCCATCACC AAGCCACCTG CCAAGGACAA GGCCGTGCTC 
AACAGCGTCA GCAGGACTGC CTTGAGCGAG AAGAAGCCCA CTGTGAAGCC GAAGTCACCG 
GAAAAGAGCA AGCCGGACGA AAAGGACCCG GAAAAGTCAC CCACCAAAAA ACAAGAAGTC 
CCTGAGGAAA AGTACCTGAC ATTGGAAGGA TTTCACAAGT TTGTTATTGA CCGAGCCAAG 
CAAGACATCC GTAGTGTCTG GAGGGCGATT TTATCCTGTG GTTACGATCT TCATTTTGAG 
AGGTGTGCCT GCATCGACGT CCGGCACGCA CACAAGGCCT CCAGGAAGTG GACCCTGGAG 
ATGGACGTGG CGCTCGTGCA GTACATCAAC CGGCTGTGCC GCCACCTGGC CATCACGCCC 
GCGCGGCTGC ATCCCCACGA GGTGTATCTG GAGACCACAG ACGCCGCCGA CCCCAGAGTG 
GCCTGTCTCT TGAATGTGCC CATTGAGAGC CTGCGCCTGC GCTTCGCCCT GCTGCAGTCC 
CTGAACACCA CGCTCGAGAC CTTCTTCCTG CCACTCGTGG AGCTGCGCCA GACGCCCATG 
TACACGCACA GCATCGCGGC CCTGCTGAAG GAGGCCAAAG GGCTGATCTT TTATGACACG 
AAGGTGACCG TCATGAATCG GGTGCTGAAC GCCACAGTGC AGAGGACGGC TGACCACGCG 
GCACCGGAGA TTACTCTGGA CCCACTGGAG ATCGTGGGAG GGGAGATCAG AGCCTCTGAA 
AACTCCTACT TCTGCCAGGC GGCCAGGCAG CTGGCCTCGG TGCCATCCTC CCAGCTTTGC 
GTCAGACTGG CGAGTGGCGG TGACCCCACC TATGCCTTCA ACATCCGCTT CACTGGGGAG 
GAGGTCCACG GCACCAGCGG CTCCTTCCGC CACTTCCTGT GGCAGGTGTG TAAAGAGCTG 
CAGAGCTCCG CGCTCTCCCT TCTCCTGCTG TGCCCCAGCT CAGCCGTCAA CAAGAACAAG 
GGCCAGTACA TCCTGAGCCC AAGCCCCATC AGCTACGGGG AGGAGCAGCT GCTGCACTTC 
CTGGGCCAGT TGCTGGGAAT TGCGATTCGA GCAGATGTCC CTCTTCCCCT GGACCTCCTA 
CCCTCCTTCT GGAAGACGCT GGTGGGCGAG CCCCTGGACC CTGACCAAGA CCTGCAGGAA 
GCAGACATAC TCACCTACAA TTATGTCAAG AAATTCGAGA GTATCAACGA CGAGAGCGAG 
CTGGAGGCCC TGTGCGCCGA GATTGCCTCC CAGCACCTGG CCATCGAGAG CCCCGACGGG 
CCCAGTAAGC CCTGCTGCCG GTTCACCTAC CTGACCATGG CGGGCGAGGA GGTGGAGCTG 
TGCAGCCGCG GCCGGCACAT CCCTGTGGCG TGGGAGAACA AGGACATCTA CGCAGCAGCC 
ATCCGGAGCC TGCGGCTGCG GGAGCTGCAG AACGTCGAGT GTGTGACAGC CGTGCGCGCG 
GGCCTCGGCG CCATCATCCC CCTGCAGCTG CTCACCACGC TCAGCCCGCT GGAGATGGAG 
CTGCGGACGT GCGGCCTCCC TTACATCAAC CTCGAGTTCC TGAAGGCCCA CACCATGTAC 
CAGGTGGGCC TGATGGAGAC GGACCAGCAC ATCGAGTTCT TCTGGGGGGC ACTGGAGATG 
TTTGCCCAGG AGGAGCTGTG CAAGTTCATC AAGTTTGCCT GCAACCAAGA GCGCATCCCG 
TTCACCTGCC CCTGCAAAGA CGGGGGCCCC GACACAGCCC ACGTGCCCCC ATACCCCATG 
AAGATCGCCC CCCCGGATGG CACAGCAGGT TCCCCAGACT CTCGCTACAT CCGAGTGGAG 
ACCTGCATGT TCATGATCAA GCTTCCCCAG TACTCCTCTC TGGAAATCAT GCTGGAGAAA 
CTTCGTTGTG CCATTCACTA TCGCGAAGAC CCCCTGAGTG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007114398.1
CDS	47..11911
Translation	MRKEGESAKSINEMLLSRLSRYRASPSATLAALTGSTISNTLKEDQAANTSCGLPLKMLRKTPIYTCGTYLVMLVPPPGGSGSSATRSLFGGTSGLSSLKILASSLVYNISDGQFASRADLIDAAGSSLGRGALVPGLGACYDTVNNMLWTCSNDYIDQWCNPGNQAFHYVCQRLGVSHIITEPKEEAITTNEVINQLLHHVGAMCIHQLNLLATNPNLPITSVLGKQHPIEAHHLSSICDIMEKAMVNGDTCIIRCILVVFQVVFKFFFSPQTERNRDIIRRSGLLLWQLLMAPKDQICPEIQKEVCLAISSGLNILYPGETEINNLLKLVLTEGERNSGLSQLRDVILTNLAEQLQNNRFGSEEDDHYRLNDELLHYILKIVVRESCILITKCQTVSKDDFQKLLSTVPAASSCLRYLMAVQNHLLSNTVLIKPDENDDSDNSLQGETLKELKVSILALATQILTGCDEVLEMLQQVTTALINSDIADREQRLKGLEQVTKATMLGHLLPVLLTSLMHPNLQTLTMADALMPQLVQLVLYTSQTALLLKTQCPVFAEAGCSPCGASDQKGRLFPDERMLEEKEEPGFLTGLKIPAPWAAGKTVETVHPVRDNYKFKETVHIPGARCLYLRFDSRCSSQYDYDKLVIYAGPNTNSRKVAEYGGNTLGYGSRSVLGTGWPKDLVKVEGDTVTFSFEMRSGREHNTPDKAMWGFACTVRAQESSEDVSGGLPFLVDLALGLSVLACSMLRILYNGPEITKEEEACQELLRSKLLQRCQWQVEANGVISPALTPSPSPLPLTIEEDREFTYPSDVLVPPVGNYFDLPRIRLPPGIMIKLREISGRARPQFRPSIKEVIQPDVMEEMVVSCVIKHLNLVDALQSLINFQYQEEHAEEYDLLCKIMGETFKKLNAMERQLQSVAELEQKWQSEVDDATQGKLENNMPFFYDYHFNENKMKELELLCSMKEVSFDGNDLENMVLSLREKFLQEVNSLIQKPSHPLAKTKTLVKSLMNRAELLLHVTIAAQSGLTRSISGTPAETPACKSASETKVISHAVRQPVFLRSMSAPSDLEMIGNDDLEFTRANQRRRHVTSHRSSSFTLLQSLAIEDSRDKPTYSVLLGQLFAFIGTNPDQAVSSSSFLLAAQTRWRRGNTRKQALVHMRELLTAAVRVGGVTHLVGPVTMVLQGGPRIEELTCGGMVEQVQEAFGETMTSVVSLCARYPIACANSIGLLCTIPYTRSEEKCLVRSGLVQLMDRLCSLSSQTESSSSEKQTKKQKVATMAWAAFQVLANRCVEWEKEEGGSTEAVHSGLARQVSSLLTNHLARATESCGNQAAGNDALQDVLSLLNDLSRSHIGKAILSQPACVSKLLSLLLDQRPSPKLVLIILQLCRAALPLMSVEDCGNVELPPWSYSVPSLNSEQEDPSDPASKIASLLLAKLADYVVPGCQTVLSPTASEPDTMLTKTSPKSSLKGDKDPGEESEAVDGKLSIFIHKREDQSSHEVLQPLLSSSEGRPFRLGTGANMEKVVKMDRDMTKGGCCEVITEEAAAALRKATKWAQSGLIVSVGPPVESLNPETVSGLSTGDKKKTAQTSICRERNSELARTDPVRPFISGHVANSMAAEVIALLHSLLMAPESNAAQIWTTTAEKVLSRALMYIPQLGKYAESILENGSSSGRKLAKLQRIARQAVAALCALGGFKETIKIGSEVQVLGRGISGSVGVVASINEQEGIATVKFPPIDCRKTSQASDTLTIPLSRLCVPRSEALPLHKLSITEKVVQAVQSMLLPQEGSLSIHTSLPATGDGSAPVMAVVRLLAEIRTRACLVMAQLLEDSLFCEEFIQQCPAAVEVLNLVAQECSAGERLAVVEVQCERLRMLYRDCARPPPPPLQADRRQPKEITWSPSRVFPPVRACMFSSHLTSVTFLADPSAGGGLPRGTFIYATSPLPIQAPSFYWEIEIVSYGDSDDDTGPIVSFGFATEAEKRDGAWTNPVGTCLFHNNGRAVHYNGSSLLQWKSVRLDVTLSPGDVAGIGWERTEGTPPPPGQPAKGRVYFTYCGQRLSPYLEDVSGGMWPVVHIQKKNTKTRANFGSRPFAYAEGQAHRNAADLCTDLAEEISANFEALPFAMASDSDNDAGTSIASDPGTHGPPCRIAAVATAQQQYDSDTSCHYKVELSYENFITSGPDPHPPPIADDESDDDDDDDIPQEDHYALLVKAWETKVFPTIRRRFRNEAERKSGLDQIKGALQLGMVDIARQTVEFLYEENGGIPRDLYLPTIEDIKDEANKFTIDKVRKGLTVVTRSPDSNNVASSTVGTALPKFAIRGMLKTFGLHGVVLDVDSVNELVQVETYLRSEGVLVRYWYPIDMLERPPAGYRRTATNGLVTLDNTNLQIHRELLRCEAALARLYCRMALLNIFAPRLPHLFTRLFHIPAIRDITLEHLQLLSNQLLAPPLPDGTISSSSILLAQSLQHCIHSQNCSATDLFYQGSSQTVREWLNVAITRTLHQGEESLLELTKQICSFLQTAPEQFPSEEFPISESKVNMDVNFPGAAFVVVSCKESQSGFRKDSSLYKAPWARVLVYGLGHKVKRNGQLNLIEAICYPRDASPANTGLTPPPTTNQYPSVILSTDKVHIKLGVSPPPGAVLVLHSLPLEFPLAMAFAEQLLSWKSEDGEGRSEDEPDTIPTSVLLQVVELLGNFLWTTDMAACVKELVFHLLAELVRTVHSLEQRKHPAGLSSSIALQLNPCLAMLMALQSELHKLYDEETQSWVSGSTCGGSGTVAATDQGRFSTYFHALMEGCLAVAEVTLPTNMSVTASGVTSATAPNLSDSSSSSSSSPGQTPQSPSLLSKRKKVKMKREKASSSGKRQSSHSVDSDPAVLSIGGGKPEDMLWFHRALTLLIILRHLTRKDPQGLGVTSDAIADACQALVGPTAHSRLLVISGIPTHLDEGIVRGAIRKACNAHGGVFKDEIYIPLQDEDPKKPKDKAEGGDGKAEPEKTLGFPSTDSLEVSTSRSPTPAMSISASASTSQASICSSQGILQTVSDVSVEPLPSGLELPIPAGLLEPHVVSSQESLDVSLCSTSSLGSLGSLGEPLDNAETASVSDMGSMYTVTSLDNQPLAARPIKGFAVVEIRSRAKIEKIRASLFNNNDLIGLSSLDGEDELMEMSTEEILTVSVVNQSLFDTQGSPGLEDYFNDKSIKGEKLVPGAREVLTEIFKSCAHSEQMLSLTPAKPIKVSDIYLSKEQINSQTPGNLLHLFFTNVRPPKKVLEDQLTQILRKYGVPKPKFDKSKYSKAGKEQHPGKVVSTKRPITKPPAKDKAVLNSVSRTALSEKKPTVKPKSPEKSKPDEKDPEKSPTKKQEVPEEKYLTLEGFHKFVIDRAKQDIRSVWRAILSCGYDLHFERCACIDVRHAHKASRKWTLEMDVALVQYINRLCRHLAITPARLHPHEVYLETTDAADPRVACLLNVPIESLRLRFALLQSLNTTLETFFLPLVELRQTPMYTHSIAALLKEAKGLIFYDTKVTVMNRVLNATVQRTADHAAPEITLDPLEIVGGEIRASENSYFCQAARQLASVPSSQLCVRLASGGDPTYAFNIRFTGEEVHGTSGSFRHFLWQVCKELQSSALSLLLLCPSSAVNKNKGQYILSPSPISYGEEQLLHFLGQLLGIAIRADVPLPLDLLPSFWKTLVGEPLDPDQDLQEADILTYNYVKKFESINDESELEALCAEIASQHLAIESPDGPSKPCCRFTYLTMAGEEVELCSRGRHIPVAWENKDIYAAAIRSLRLRELQNVECVTAVRAGLGAIIPLQLLTTLSPLEMELRTCGLPYINLEFLKAHTMYQVGLMETDQHIEFFWGALEMFAQEELCKFIKFACNQERIPFTCPCKDGGPDTAHVPPYPMKIAPPDGTAGSPDSRYIRVETCMFMIKLPQYSSLEIMLEKLRCAIHYREDPLSG

Target ORF information:

RefSeq Version	XM_007114336.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007114336.1

ORF Insert Sequence:

ATGAGAAAAG AGGGCGAGTC TGCAAAAAGC ATTAATGAGA TGCTTCTGAG CAGACTTTCA 
CGCTACCGAG CCTCGCCATC CGCAACGCTG GCTGCCCTTA CCGGCTCCAC CATCTCCAAC 
ACCCTGAAAG AGGACCAGGC AGCAAATACT AGCTGTGGGC TTCCTCTAAA AATGCTTCGG 
AAGACGCCCA TTTACACCTG TGGTACATAC TTGGTGATGC TGGTCCCACC TCCAGGGGGA 
TCAGGCAGCT CTGCTACCCG GTCTCTTTTT GGTGGAACAA GTGGTTTGTC GTCTTTAAAA 
ATCCTAGCTT CTAGCTTGGT GTATAATATT TCGGATGGTC AATTCGCAAG TCGTGCTGAT 
CTCATAGATG CTGCTGGAAG CTCATTGGGG CGTGGTGCTC TTGTACCAGG ATTAGGTGCC 
TGTTACGATA CAGTGAACAA CATGCTATGG ACATGCTCGA ATGACTATAT CGATCAATGG 
TGTAATCCTG GGAATCAGGC CTTCCATTAC GTCTGTCAGA GACTTGGAGT CAGCCATATC 
ATCACTGAGC CTAAAGAAGA GGCTATAACC ACGAATGAGG TTATAAACCA ATTATTGCAC 
CACGTTGGTG CGATGTGCAT ACACCAACTC AATCTTCTTG CTACCAACCC CAATCTTCCA 
ATCACAAGTG TCTTGGGCAA GCAGCATCCA ATTGAAGCAC ACCATCTTAG CAGTATTTGT 
GACATTATGG AGAAGGCCAT GGTTAATGGA GACACCTGTA TCATACGCTG CATTCTCGTT 
GTCTTTCAGG TGGTGTTTAA ATTTTTCTTC AGCCCACAAA CTGAAAGGAA TCGAGACATC 
ATTCGACGGT CGGGGTTGCT TCTTTGGCAG TTGTTGATGG CACCAAAAGA TCAAATTTGT 
CCTGAAATTC AGAAGGAAGT CTGCCTTGCC ATCAGCTCTG GTTTAAATAT CTTGTACCCA 
GGTGAAACTG AAATCAATAA CTTACTTAAG CTGGTCTTAA CAGAAGGAGA GAGAAACAGT 
GGCCTCTCCC AGCTGCGGGA TGTGATCCTA ACCAATTTAG CTGAACAGCT TCAAAACAAC 
CGATTTGGCA GTGAGGAGGA TGATCATTAC AGACTAAATG ACGAACTTTT ACACTACATT 
TTGAAGATTG TTGTACGAGA ATCCTGTATC TTAATCACCA AGTGCCAAAC TGTCTCTAAA 
GATGATTTTC AAAAACTCCT TTCAACTGTG CCTGCTGCAT CCTCCTGCCT GCGCTATCTG 
ATGGCAGTTC AGAACCACCT TCTCAGTAAC ACTGTTTTGA TTAAACCTGA TGAGAATGAT 
GACAGTGACA ACTCCTTGCA GGGAGAGACA TTGAAGGAGC TAAAGGTCAG TATTTTGGCT 
CTTGCCACCC AAATCCTGAC TGGATGTGAT GAAGTGTTGG AAATGCTACA GCAGGTCACA 
ACTGCCCTCA TAAATAGTGA CATAGCTGAC CGGGAGCAGA GGTTAAAAGG CTTGGAACAA 
GTTACTAAGG CTACTATGCT CGGTCACCTT CTTCCTGTGT TACTGACCTC CCTGATGCAT 
CCAAATTTAC AGACTCTGAC CATGGCCGAT GCCCTGATGC CTCAGCTAGT GCAGCTGGTA 
CTTTATACCA GTCAGACGGC TTTGCTGCTC AAAACCCAGT GTCCGGTTTT TGCTGAGGCG 
GGCTGTTCCC CATGTGGTGC ATCAGACCAG AAGGGCAGGC TGTTCCCTGA TGAGAGAATG 
CTGGAAGAGA AGGAAGAGCC AGGGTTTCTC ACTGGTTTAA AGATTCCTGC TCCATGGGCT 
GCTGGGAAGA CTGTGGAAAC AGTCCATCCT GTCAGAGACA ACTATAAATT TAAAGAAACT 
GTCCATATCC CGGGAGCTCG CTGCCTATAC CTTAGATTTG ATAGCAGATG CTCTTCACAA 
TATGACTATG ACAAATTGGT GATATACGCG GGGCCTAACA CAAACAGTAG GAAGGTTGCT 
GAGTATGGAG GCAACACTCT GGGATATGGC AGCCGTAGTG TCTTAGGAAC TGGTTGGCCG 
AAAGACTTGG TGAAGGTGGA AGGAGATACA GTCACCTTCT CCTTTGAAAT GAGAAGTGGC 
CGTGAACACA ACACTCCCGA TAAAGCTATG TGGGGCTTTG CTTGCACAGT TCGCGCTCAG 
GAGTCTTCAG AGGATGTGTC GGGAGGCTTA CCTTTTCTGG TAGACCTGGC TTTAGGTCTG 
TCTGTGTTAG CTTGTTCTAT GTTAAGAATC CTATACAACG GACCAGAAAT TACCAAAGAA 
GAAGAAGCCT GTCAGGAGCT GCTGCGGTCC AAACTTTTAC AAAGGTGCCA GTGGCAGGTG 
GAGGCCAATG GTGTGATCTC CCCTGCCCTT ACTCCAAGTC CGTCTCCACT GCCTCTGACC 
ATCGAGGAAG ACAGAGAATT CACTTACCCC TCTGACGTCC TCGTGCCTCC TGTTGGAAAC 
TACTTTGACC TGCCCCGGAT CAGGCTGCCT CCAGGAATCA TGATAAAGCT CAGGGAAATT 
TCTGGGCGTG CCAGACCTCA ATTTAGACCA AGTATAAAAG AAGTAATCCA GCCAGACGTG 
ATGGAGGAAA TGGTGGTATC GTGTGTCATT AAGCACTTGA ACTTGGTTGA TGCACTGCAG 
TCCCTAATAA ATTTCCAGTA TCAAGAAGAA CATGCTGAGG AATATGATTT ATTGTGTAAA 
ATCATGGGAG AGACATTTAA GAAACTCAAT GCCATGGAGA GACAGCTGCA GAGTGTTGCA 
GAATTGGAAC AGAAATGGCA AAGTGAGGTT GATGATGCCA CGCAGGGGAA ACTGGAGAAC 
AACATGCCTT TCTTCTATGA TTACCATTTT AATGAGAACA AAATGAAAGA ACTAGAACTT 
TTGTGTTCAA TGAAGGAAGT CTCCTTTGAT GGAAATGATC TTGAAAACAT GGTCCTATCT 
CTGAGGGAGA AGTTCCTACA AGAAGTGAAT TCTCTCATTC AGAAACCCTC GCACCCACTG 
GCTAAGACGA AGACGTTAGT GAAGAGTCTA ATGAACCGAG CTGAGCTGTT GCTGCACGTC 
ACCATTGCGG CCCAGTCCGG CCTCACAAGA AGCATCTCAG GGACCCCCGC AGAAACACCG 
GCTTGTAAAT CAGCCTCTGA AACAAAGGTG ATATCTCATG CTGTGAGGCA GCCTGTTTTT 
CTTCGCAGCA TGTCGGCTCC TTCTGACCTG GAAATGATTG GTAACGATGA TTTGGAATTT 
ACTAGAGCAA ATCAGAGGCG GCGCCACGTG ACCAGCCACC GCAGCAGCTC CTTCACGCTC 
CTGCAGTCAT TGGCCATCGA GGACAGCAGG GACAAGCCCA CCTACAGCGT CCTGCTGGGG 
CAGCTGTTTG CTTTCATCGG CACCAACCCT GACCAAGCTG TGTCCAGCAG CAGCTTTCTT 
TTGGCTGCGC AGACAAGGTG GCGACGGGGA AACACTCGCA AGCAGGCACT GGTGCACATG 
CGGGAATTGC TGACGGCTGC CGTGCGAGTC GGGGGAGTGA CACATCTTGT GGGTCCGGTG 
ACTATGGTCC TTCAGGGAGG ACCCAGAATT GAAGAACTAA CCTGTGGTGG GATGGTCGAG 
CAGGTCCAGG AAGCCTTCGG TGAGACCATG ACCTCTGTTG TGTCTTTGTG CGCCCGTTAC 
CCTATTGCTT GTGCGAATAG CATCGGACTC TTATGTACTA TACCTTACAC CAGGAGTGAA 
GAGAAGTGCC TGGTGCGCAG TGGCCTTGTT CAGCTCATGG ATCGACTGTG TAGCTTGAGC 
AGTCAGACTG AGTCCAGCTC CAGTGAGAAA CAAACCAAGA AGCAGAAGGT GGCTACCATG 
GCCTGGGCAG CCTTCCAGGT GCTTGCCAAC CGCTGCGTTG AATGGGAAAA AGAAGAAGGC 
GGCTCCACAG AGGCTGTGCA CTCAGGTCTG GCCCGTCAGG TGTCCAGCCT TCTCACTAAC 
CACCTCGCCC GAGCGACGGA GAGCTGCGGC AACCAGGCTG CCGGGAACGA TGCCCTCCAG 
GACGTCCTGA GTCTTCTCAA TGACCTCTCG AGGAGTCACA TAGGTAAAGC CATCCTGAGC 
CAGCCAGCTT GTGTGTCCAA ACTCCTCTCC CTGCTGCTTG ACCAACGCCC GTCCCCAAAG 
CTGGTCCTTA TCATTCTTCA GCTGTGCCGG GCAGCACTCC CGCTGATGAG CGTAGAAGAC 
TGTGGGAATG TGGAGCTCCC ACCCTGGAGC TACTCTGTCC CCTCCCTAAA CAGTGAGCAA 
GAGGATCCCA GCGACCCAGC GTCCAAGATC GCCTCTTTGC TCTTAGCAAA GCTAGCAGAT 
TATGTGGTCC CAGGATGTCA GACAGTCCTT TCTCCAACCG CTTCTGAACC TGACACCATG 
TTGACAAAAA CCAGCCCCAA GAGTTCCTTA AAAGGAGATA AAGACCCTGG AGAAGAGAGT 
GAAGCAGTGG ATGGCAAGCT CTCTATCTTT ATCCACAAGC GGGAAGACCA GTCATCCCAT 
GAAGTTCTCC AACCATTACT AAGTAGCTCA GAAGGACGGC CCTTCCGACT TGGTACTGGT 
GCCAACATGG AGAAAGTCGT GAAAATGGAC CGAGACATGA CAAAGGGTGG CTGCTGTGAG 
GTGATTACAG AAGAGGCGGC AGCGGCTCTG CGAAAAGCAA CCAAGTGGGC ACAGTCTGGC 
CTCATCGTCA GCGTCGGGCC ACCCGTGGAA TCGCTCAACC CAGAGACTGT GAGTGGACTG 
TCCACAGGGG ACAAGAAGAA AACCGCCCAA ACTTCCATTT GCCGAGAGAG GAACTCTGAA 
CTTGCCAGGA CCGACCCTGT CCGACCTTTC ATCAGTGGGC ATGTGGCAAA CAGCATGGCT 
GCAGAGGTGA TCGCCTTGCT TCACAGCTTG CTGATGGCGC CTGAGTCAAA TGCTGCTCAG 
ATATGGACCA CGACAGCAGA GAAGGTTCTG TCTCGTGCGC TGATGTACAT TCCACAGTTG 
GGGAAATATG CAGAAAGCAT TCTGGAAAAT GGCAGCAGTA GTGGCAGAAA ACTCGCCAAA 
CTTCAGAGAA TCGCCCGCCA GGCTGTCGCT GCACTGTGTG CACTTGGAGG CTTCAAAGAG 
ACAATCAAGA TAGGGTCTGA GGTTCAGGTT TTAGGTAGAG GAATATCAGG AAGTGTCGGA 
GTAGTGGCTT CTATCAATGA ACAGGAAGGT ATAGCTACAG TCAAATTCCC ACCCATAGAC 
TGTAGAAAGA CGTCGCAAGC ATCAGACACA CTGACTATTC CATTGTCTAG ACTTTGTGTT 
CCAAGATCAG AGGCATTGCC TCTTCATAAA CTGTCCATTA CTGAGAAGGT AGTACAGGCA 
GTCCAGTCCA TGTTGCTTCC TCAGGAGGGA AGTCTCTCTA TTCATACCTC ACTTCCTGCA 
ACAGGAGATG GGTCAGCTCC TGTGATGGCA GTCGTTCGGC TCCTGGCTGA AATAAGAACA 
AGAGCATGCC TGGTCATGGC CCAGCTTTTA GAAGACAGCT TATTTTGTGA AGAATTCATA 
CAGCAGTGTC CAGCAGCTGT TGAGGTTCTC AACCTAGTAG CCCAGGAATG CAGTGCTGGA 
GAGCGACTGG CAGTTGTGGA AGTGCAGTGT GAACGGCTGA GGATGCTCTA CCGGGACTGT 
GCTCGGCCCC CGCCACCTCC GCTGCAGGCT GACCGGAGAC AGCCCAAAGA GATTACGTGG 
AGCCCCTCAC GAGTGTTTCC ACCTGTCCGA GCCTGCATGT TCTCATCGCA CCTCACTTCC 
GTCACCTTCC TGGCTGACCC CAGCGCTGGG GGCGGTCTGC CCCGGGGCAC ATTTATCTAT 
GCCACCTCAC CACTGCCCAT TCAGGCCCCA TCTTTCTACT GGGAAATTGA AATTGTTTCC 
TATGGAGATA GTGATGATGA CACTGGACCA ATAGTTTCCT TTGGCTTTGC TACAGAAGCA 
GAGAAAAGAG ACGGGGCTTG GACTAATCCA GTGGGCACTT GTCTTTTCCA TAACAATGGC 
CGAGCCGTGC ACTACAACGG CTCCAGTTTA TTACAGTGGA AGAGCGTTCG CTTAGACGTG 
ACTCTCTCTC CTGGTGACGT GGCAGGAATT GGCTGGGAGA GAACCGAGGG AACGCCTCCT 
CCTCCAGGGC AGCCAGCCAA GGGCCGAGTG TACTTCACAT ACTGCGGGCA GCGCCTCTCA 
CCGTATCTTG AAGATGTTTC GGGTGGGATG TGGCCTGTGG TTCACATTCA GAAAAAGAAC 
ACCAAGACGC GAGCTAACTT TGGCTCCCGC CCATTTGCCT ATGCTGAAGG GCAGGCCCAC 
CGCAACGCAG CTGACCTGTG CACCGACCTG GCAGAGGAGA TCAGCGCTAA CTTCGAGGCC 
CTGCCCTTTG CCATGGCATC TGACAGTGAC AACGATGCTG GCACCAGTAT TGCGTCAGAC 
CCGGGCACTC ACGGGCCACC GTGCCGGATT GCTGCCGTGG CCACGGCTCA GCAACAATAT 
GATAGTGACA CTTCCTGTCA TTATAAAGTA GAGCTCAGCT ATGAGAATTT CATCACCTCC 
GGCCCAGACC CCCATCCGCC CCCCATTGCA GATGACGAAA GTGATGATGA TGATGACGAT 
GATATCCCGC AGGAGGACCA CTATGCTCTA CTGGTGAAAG CGTGGGAGAC CAAGGTTTTT 
CCTACTATCC GAAGACGCTT CCGCAACGAA GCAGAGCGGA AATCGGGCCT GGACCAGATA 
AAGGGTGCTT TACAACTAGG CATGGTGGAT ATTGCCCGGC AGACAGTTGA ATTCCTCTAC 
GAGGAGAACG GTGGCATCCC CAGAGACCTT TATCTTCCCA CCATTGAAGA CATTAAGGAT 
GAAGCAAACA AGTTCACAAT TGATAAAGTT CGAAAAGGTC TCACGGTAGT AACCCGCTCT 
CCAGACAGCA ATAACGTAGC CAGCAGTACT GTTGGAACTG CCCTGCCAAA ATTTGCCATC 
CGAGGGATGC TGAAAACCTT TGGGCTTCAT GGAGTTGTCT TAGATGTTGA TTCAGTAAAT 
GAATTGGTGC AGGTAGAAAC GTACCTCCGA AGCGAAGGTG TGCTGGTCCG ATACTGGTAT 
CCTATTGATA TGTTGGAAAG GCCCCCAGCA GGCTACCGAA GGACTGCCAC CAATGGGCTG 
GTCACCCTGG ACAATACCAA CCTTCAAATT CACAGGGAGC TGCTGCGCTG CGAGGCCGCA 
CTGGCCAGGC TGTACTGCCG CATGGCCCTG CTCAACATCT TCGCCCCCAG GCTGCCTCAC 
CTGTTTACCC GCCTCTTCCA CATCCCTGCC ATCCGGGACA TTACCCTGGA GCACCTGCAG 
CTGCTGTCCA ATCAGCTCCT CGCTCCTCCC CTCCCAGATG GCACCATCAG CTCCAGCTCC 
ATCCTCCTGG CACAGTCGCT GCAGCACTGC ATCCATTCCC AGAACTGCTC GGCCACGGAC 
CTCTTCTACC AGGGCAGCTC CCAGACAGTG AGAGAGTGGC TCAACGTGGC CATCACTCGG 
ACCCTGCACC AGGGTGAGGA GAGCCTGTTG GAGCTGACGA AACAGATCTG CTCTTTCCTG 
CAGACGGCAC CAGAGCAGTT CCCCTCCGAA GAGTTTCCAA TTTCCGAGTC CAAGGTCAAC 
ATGGACGTCA ATTTCCCCGG GGCGGCTTTC GTCGTGGTAT CTTGCAAAGA AAGTCAGTCT 
GGATTCCGCA AAGACTCCTC TCTGTACAAG GCGCCGTGGG CGCGCGTGCT CGTGTACGGA 
CTGGGCCACA AAGTGAAGCG AAATGGCCAG CTAAACCTCA TCGAGGCCAT CTGTTACCCG 
CGGGATGCGT CTCCGGCCAA CACGGGGCTC ACGCCGCCTC CCACCACCAA CCAGTACCCT 
TCTGTGATCC TCTCCACAGA CAAAGTCCAC ATCAAACTGG GGGTGTCTCC CCCTCCTGGA 
GCCGTGCTGG TGTTACACTC TCTGCCCCTT GAGTTCCCAC TGGCGATGGC CTTCGCAGAG 
CAGCTGCTGT CCTGGAAGTC GGAGGACGGT GAAGGGAGAT CTGAAGACGA GCCTGACACC 
ATCCCGACGT CCGTCCTCCT GCAAGTGGTG GAACTGCTCG GAAACTTCCT GTGGACCACG 
GACATGGCGG CCTGTGTGAA AGAGCTGGTT TTCCACCTCC TGGCAGAGCT CGTGCGCACA 
GTGCACAGCC TGGAGCAGAG GAAGCACCCT GCCGGCCTGT CCTCGTCCAT CGCCCTCCAG 
CTGAACCCCT GCCTGGCCAT GCTGATGGCC CTGCAGTCAG AGCTCCACAA GCTGTACGAC 
GAGGAGACGC AGAGCTGGGT CTCGGGCAGC ACGTGCGGAG GCTCGGGGAC CGTGGCGGCC 
ACCGACCAGG GCAGGTTCTC TACGTATTTC CACGCGCTCA TGGAAGGGTG CCTGGCGGTG 
GCCGAGGTGA CCCTGCCTAC CAACATGAGC GTCACTGCCA GCGGGGTGAC GTCGGCGACC 
GCCCCAAACC TCAGTGACTC GTCGTCCTCC TCGTCATCCT CCCCAGGACA GACGCCGCAG 
AGCCCCAGCC TCCTGTCCAA GAGGAAGAAA GTCAAGATGA AGCGGGAAAA GGCCTCCTCC 
TCTGGGAAGC GCCAGTCCTC GCACTCAGTG GACTCGGACC CCGCCGTGCT GAGCATCGGG 
GGTGGCAAGC CCGAAGACAT GCTGTGGTTC CATCGCGCCC TGACCTTGCT CATCATCCTC 
CGGCACCTCA CCAGGAAGGA CCCGCAGGGG CTGGGTGTGA CGAGCGACGC CATCGCCGAC 
GCCTGCCAGG CCCTGGTGGG TCCCACCGCC CACAGCCGCT TGCTGGTGAT CTCTGGGATC 
CCCACCCACC TGGACGAGGG CATCGTCAGA GGCGCCATCC GCAAGGCCTG CAACGCCCAC 
GGCGGGGTCT TCAAAGACGA GATCTACATC CCCCTGCAGG ACGAAGACCC CAAGAAGCCA 
AAAGACAAGG CCGAGGGCGG TGATGGGAAA GCCGAGCCAG AGAAGACCCT TGGCTTCCCC 
AGCACAGACA GCTTGGAGGT CAGCACGTCC CGCAGCCCGA CCCCCGCCAT GAGCATCAGC 
GCGTCTGCGT CCACCAGCCA AGCCTCCATC TGCAGCTCCC AGGGCATCTT GCAGACAGTC 
AGCGATGTCT CTGTGGAACC GCTGCCCTCG GGCCTCGAGC TGCCCATCCC TGCGGGCTTA 
TTGGAGCCCC ACGTAGTGTC CAGCCAGGAG AGCCTGGACG TTTCCCTGTG CAGCACCAGC 
AGCCTGGGCA GCCTCGGGAG CCTGGGGGAG CCACTGGACA ACGCAGAAAC GGCCTCAGTG 
TCAGACATGG GCTCCATGTA CACAGTCACG TCCCTGGACA ACCAGCCCCT CGCAGCACGC 
CCCATCAAAG GCTTCGCGGT CGTGGAGATT AGATCCCGAG CCAAAATCGA GAAAATCCGA 
GCGAGTTTAT TTAACAATAA TGACTTGATT GGTTTGTCAA GTTTGGATGG TGAAGATGAA 
TTGATGGAAA TGTCAACGGA AGAGATCCTC ACTGTGTCTG TAGTAAATCA GAGTTTGTTT 
GACACGCAGG GGAGCCCAGG GTTAGAAGAT TATTTCAATG ATAAGTCAAT TAAAGGTGAG 
AAGCTGGTGC CCGGGGCCAG AGAGGTGCTG ACGGAGATAT TTAAGAGCTG CGCCCACTCA 
GAGCAGATGC TGAGTCTGAC ACCAGCAAAG CCCATCAAAG TGTCTGACAT TTATCTCAGC 
AAAGAGCAGA TCAACTCCCA GACGCCAGGC AACCTCCTCC ACCTCTTCTT CACCAATGTT 
CGGCCCCCCA AAAAGGTGCT GGAGGACCAG CTCACCCAGA TCCTGAGGAA GTACGGCGTG 
CCAAAACCCA AGTTCGACAA GAGCAAGTAC AGCAAGGCGG GGAAGGAACA GCACCCCGGG 
AAGGTGGTGA GCACCAAGCG GCCCATCACC AAGCCACCTG CCAAGGACAA GGCCGTGCTC 
AACAGCGTCA GCAGGACTGC CTTGAGCGAG AAGAAGCCCA CTGTGAAGCC GAAGTCACCG 
GAAAAGAGCA AGCCGGACGA AAAGGACCCG GAAAAGTCAC CCACCAAAAA ACAAGAAGTC 
CCTGAGGAAA AGTACCTGAC ATTGGAAGGA TTTCACAAGT TTGTTATTGA CCGAGCCAAG 
CAAGACATCC GTAGTGTCTG GAGGGCGATT TTATCCTGTG GTTACGATCT TCATTTTGAG 
AGGTGTGCCT GCATCGACGT CCGGCACGCA CACAAGGCCT CCAGGAAGTG GACCCTGGAG 
ATGGACGTGG CGCTCGTGCA GTACATCAAC CGGCTGTGCC GCCACCTGGC CATCACGCCC 
GCGCGGCTGC ATCCCCACGA GGTGTATCTG GAGACCACAG ACGCCGCCGA CCCCAGAGTG 
GCCTGTCTCT TGAATGTGCC CATTGAGAGC CTGCGCCTGC GCTTCGCCCT GCTGCAGTCC 
CTGAACACCA CGCTCGAGAC CTTCTTCCTG CCACTCGTGG AGCTGCGCCA GACGCCCATG 
TACACGCACA GCATCGCGGC CCTGCTGAAG GAGGCCAAAG GGCTGATCTT TTATGACACG 
AAGGTGACCG TCATGAATCG GGTGCTGAAC GCCACAGTGC AGAGGACGGC TGACCACGCG 
GCACCGGAGA TTACTCTGGA CCCACTGGAG ATCGTGGGAG GGGAGATCAG AGCCTCTGAA 
AACTCCTACT TCTGCCAGGC GGCCAGGCAG CTGGCCTCGG TGCCATCCTC CCAGCTTTGC 
GTCAGACTGG CGAGTGGCGG TGACCCCACC TATGCCTTCA ACATCCGCTT CACTGGGGAG 
GAGGTCCACG GCACCAGCGG CTCCTTCCGC CACTTCCTGT GGCAGGTGTG TAAAGAGCTG 
CAGAGCTCCG CGCTCTCCCT TCTCCTGCTG TGCCCCAGCT CAGCCGTCAA CAAGAACAAG 
GGCCAGTACA TCCTGAGCCC AAGCCCCATC AGCTACGGGG AGGAGCAGCT GCTGCACTTC 
CTGGGCCAGT TGCTGGGAAT TGCGATTCGA GCAGATGTCC CTCTTCCCCT GGACCTCCTA 
CCCTCCTTCT GGAAGACGCT GGTGGGCGAG CCCCTGGACC CTGACCAAGA CCTGCAGGAA 
GCAGACATAC TCACCTACAA TTATGTCAAG AAATTCGAGA GTATCAACGA CGAGAGCGAG 
CTGGAGGCCC TGTGCGCCGA GATTGCCTCC CAGCACCTGG CCATCGAGAG CCCCGACGGG 
CCCAGTAAGC CCTGCTGCCG GTTCACCTAC CTGACCATGG CGGGCGAGGA GGTGGAGCTG 
TGCAGCCGCG GCCGGCACAT CCCTGTGGCG TGGGAGAACA AGGACATCTA CGCAGCAGCC 
ATCCGGAGCC TGCGGCTGCG GGAGCTGCAG AACGTCGAGT GTGTGACAGC CGTGCGCGCG 
GGCCTCGGCG CCATCATCCC CCTGCAGCTG CTCACCACGC TCAGCCCGCT GGAGATGGAG 
CTGCGGACGT GCGGCCTCCC TTACATCAAC CTCGAGTTCC TGAAGGCCCA CACCATGTAC 
CAGGTGGGCC TGATGGAGAC GGACCAGCAC ATCGAGTTCT TCTGGGGGGC ACTGGAGATG 
TTTGCCCAGG AGGAGCTGTG CAAGTTCATC AAGTTTGCCT GCAACCAAGA GCGCATCCCG 
TTCACCTGCC CCTGCAAAGA CGGGGGCCCC GACACAGCCC ACGTGCCCCC ATACCCCATG 
AAGATCGCCC CCCCGGATGG CACAGCAGGT TCCCCAGACT CTCGCTACAT CCGAGTGGAG 
ACCTGCATGT TCATGATCAA GCTTCCCCAG TACTCCTCTC TGGAAATCAT GCTGGAGAAA 
CTTCGTTGTG CCATTCACTA TCGCGAAGAC CCCCTGAGTG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD4 ( XM_024120790.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_023976558.1 Physeter catodon HECT domain E3 ubiquitin protein ligase 4 (HECTD4), mRNA.

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CloneID	OPh289407
Clone ID Related Accession (Same CDS sequence)	XM_024120790.1
Accession Version	XM_024120790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	probable E3 ubiquitin-protein ligase HECTD4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCGT CGGCAGCCGC GGCGGCTGCG GCGGCGGCGG CCGCGGACTC GGCGCAGTGG 
CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG 
GCCGAGCTGC CCAGCGAGAT TCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTGGAGATT 
TTAACATTTG AGACCAAGAA TCCGACGGAA TTAGCAGAAC GTTTGCGCTC TGTTTGTGGG 
AATCAGAGCA ATGCGTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG 
TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAAAG TTCGGGTGAC 
GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC 
TTCACGTCGA GGATGGGGCT TCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA 
GACCCCTCTC TCTGTAACAT AACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG 
CCTTTGAGCT TGACCAAGGA GCCTGCTGAC TGTCTGAATG GAATCGAAAC TTTGCTGTGC 
TCTTGGCTAG AGGAGACTTC TGACACAGGC CGACACATCC CACATAAGCA AAAAGAAAAT 
GCTGCTGCTG CCCTGGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT 
ACAGTCCATC TATTACAGAA ACAGACAGAT CTAGGGTCCC TGCCTGTAGC CGATGTGCTG 
TATAGGCTGT TGCTTTTGGA AGGGGGACCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA 
CACATAGTAT CATGGGGGTA TGAAGACATG TTGCCTGCAC CAGATAGCAA CACTGGCTCC 
AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTGTCA CGGCAGATGG TCTTTACCTG 
TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT 
CTCAGAGGTT TTGTGTACTG CCGGAATGAG GAGCTGGAAC CAGGATGGAT TGCTTTTGGC 
AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC TCTTTTCCAG 
GTCATTGACC AGAATACCCT TCAGGTGTGC CAGATGGTGC CAATGCCAGC CAATCACCTC 
CCTGTGGGCA GCACTATGAG CACCGTGCAC CTTTCTTCAG ATGGTACTTA CTTCTATTGG 
ATCTGGTCTC CTGCCAGTCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC 
ATTTTTGAAC TTGTGGTTGA AAATGGTGTA TTTGTAGCCA ACCCGCTTCA GGAACGCACA 
ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT TCTGAGCAGA 
CTTTCACGCT ACCGAGCCTC GCCATCCGCA ACGCTGGCTG CCCTTACCGG CTCCACCATC 
TCCAACACCC TGAAAGAGGA CCAGGCAGCA AATACTAGCT GTGGGCTTCC TCTAAAAATG 
CTTCGGAAGA CGCCCATTTA CACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA 
GGGGGATCAG GCAGCTCTGC TACCCGGTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT 
TTAAAAATCC TAGCTTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CGCAAGTCGT 
GCTGATCTCA TAGATGCTGC TGGAAGCTCA TTGGGGCGTG GTGCTCTTGT ACCAGGATTA 
GGTGCCTGTT ACGATACAGT GAACAACATG CTATGGACAT GCTCGAATGA CTATATCGAT 
CAATGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GTCAGAGACT TGGAGTCAGC 
CATATCATCA CTGAGCCTAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA 
TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT 
CTTCCAATCA CAAGTGTCTT GGGCAAGCAG CATCCAATTG AAGCACACCA TCTTAGCAGT 
ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATCAT ACGCTGCATT 
CTCGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA 
GACATCATTC GACGGTCGGG GTTGCTTCTT TGGCAGTTGT TGATGGCACC AAAAGATCAA 
ATTTGTCCTG AAATTCAGAA GGAAGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG 
TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA 
AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTAACCA ATTTAGCTGA ACAGCTTCAA 
AACAACCGAT TTGGCAGTGA GGAGGATGAT CATTACAGAC TAAATGACGA ACTTTTACAC 
TACATTTTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC 
TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CTGCATCCTC CTGCCTGCGC 
TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTG TTTTGATTAA ACCTGATGAG 
AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGTACAGGA GCTAAAGGTC 
AGTATTTTGG CTCTTGCCAC CCAAATCCTG ACTGGATGTG ATGAAGTGTT GGAAATGCTA 
CAGCAGGTCA CAACTGCCCT CATAAATAGT GACATAGCTG ACCGGGAGCA GAGGTTAAAA 
GGCTTGGAAC AAGTTACTAA GGCTACTATG CTCGGTCACC TTCTTCCTGT GTTACTGACC 
TCCCTGATGC ATCCAAATTT ACAGACTCTG ACCATGGCCG ATGCCCTGAT GCCTCAGCTA 
GTGCAGCTGG TACTTTATAC CAGTCAGACG GCTTTGCTGC TCAAAACCCA GTGTCCGGTT 
TTTGCTGAGG CGGGCTGTTC CCCATGTGGT GCATCAGACC AGAAGGGCAG GCTGTTCCCT 
GATGAGAGAA TGCTGGAAGA GAAGGAAGAG CCAGGGTTTC TCACTGGTTT AAAGATTCCT 
GCTCCATGGG CTGCTGGGAA GACTGTGGAA ACAGTCCATC CTGTCAGAGA CAACTATAAA 
TTTAAAGAAA CTGTCCATAT CCCGGGAGCT CGCTGCCTAT ACCTTAGATT TGATAGCAGA 
TGCTCTTCAC AATATGACTA TGACAAATTG GTGATATACG CGGGGCCTAA CACAAACAGT 
AGGAAGGTTG CTGAGTATGG AGGCAACACT CTGGGATATG GCAGCCGTAG TGTCTTAGGA 
ACTGGTTGGC CGAAAGACTT GGTGAAGGTG GAAGGAGATA CAGTCACCTT CTCCTTTGAA 
ATGAGAAGTG GCCGTGAACA CAACACTCCC GATAAAGCTA TGTGGGGCTT TGCTTGCACA 
GTTCGCGCTC AGGAGTCTTC AGAGGATGTG TCGGGAGGCT TACCTTTTCT GGTAGACCTG 
GCTTTAGGTC TGTCTGTGTT AGCTTGTTCT ATGTTAAGAA TCCTATACAA CGGACCAGAA 
ATTACCAAAG AAGAAGAAGC CTGTCAGGAG CTGCTGCGGT CCAAACTTTT ACAAAGGTGC 
CAGTGGCAGG TGGAGGCCAA TGGTGTGATC TCCCCTGCCC TTACTCCAAG TCCGTCTCCA 
CTGCCTCTGA CCATCGAGGA AGACAGAGAA TTCACTTACC CCTCTGACGT CCTCGTGCCT 
CCTGTTGGAA ACTACTTTGA CCTGCCCCGG ATCAGGCTGC CTCCAGGAAT CATGATAAAG 
CTCAGGGAAA TTTCTGGGCG TGCCAGACCT CAATTTAGAC CAAGTATAAA AGAAGTAATC 
CAGCCAGACG TGATGGAGGA AATGGTGGTA TCGTGTGTCA TTAAGCACTT GAACTTGGTT 
GATGCACTGC AGTCCCTAAT AAATTTCCAG TATCAAGAAG AACATGCTGA GGAATATGAT 
TTATTGTGTA AAATCATGGG AGAGACATTT AAGAAACTCA ATGCCATGGA GAGACAGCTG 
CAGAGTGTTG CAGAATTGGA ACAGAAATGG CAAAGTGAGG TTGATGATGC CACGCAGGGG 
AAACTGGAGA ACAACATGCC TTTCTTCTAT GATTACCATT TTAATGAGAA CAAAATGAAA 
GAACTAGAAC TTTTGTGTTC AATGAAGGAA GTCTCCTTTG ATGGAAATGA TCTTGAAAAC 
ATGGTCCTAT CTCTGAGGGA GAAGTTCCTA CAAGAAGTGA ATTCTCTCAT TCAGAAACCC 
TCGCACCCAC TGGCTAAGAC GAAGACGTTA GTGAAGAGTC TAATGAACCG AGCTGAGCTG 
TTGCTGCACG TCACCATTGC GGCCCAGTCC GGCCTCACAA GAAGCATCTC AGGGACCCCC 
GCAGAAACAC CGGCTTGTAA ATCAGCCTCT GAAACAAAGG TGATATCTCA TGCTGTGAGG 
CAGCCTGTTT TTCTTCGCAG CATGTCGGCT CCTTCTGACC TGGAAATGAT TGGTAACGAT 
GATTTGGAAT TTACTAGAGC AAATCAGAGG CGGCGCCACG TGACCAGCCA CCGCAGCAGC 
TCCTTCACGC TCCTGCAGTC ATTGGCCATC GAGGACAGCA GGGACAAGCC CACCTACAGC 
GTCCTGCTGG GGCAGCTGTT TGCTTTCATC GGCACCAACC CTGACCAAGC TGTGTCCAGC 
AGCAGCTTTC TTTTGGCTGC GCAGACAAGG TGGCGACGGG GAAACACTCG CAAGCAGGCA 
CTGGTGCACA TGCGGGAATT GCTGACGGCT GCCGTGCGAG TCGGGGGAGT GACACATCTT 
GTGGGTCCGG TGACTATGGT CCTTCAGGGA GGACCCAGAA TTGAAGAACT AACCTGTGGT 
GGGATGGTCG AGCAGGTCCA GGAAGCCTTC GGTGAGACCA TGACCTCTGT TGTGTCTTTG 
TGCGCCCGTT ACCCTATTGC TTGTGCGAAT AGCATCGGAC TCTTATGTAC TATACCTTAC 
ACCAGGAGTG AAGAGAAGTG CCTGGTGCGC AGTGGCCTTG TTCAGCTCAT GGATCGACTG 
TGTAGCTTGA GCAGTCAGAC TGAGTCCAGC TCCAGTGAGA AACAAACCAA GAAGCAGAAG 
GTGGCTACCA TGGCCTGGGC AGCCTTCCAG GTGCTTGCCA ACCGCTGCGT TGAATGGGAA 
AAAGAAGAAG GCGGCTCCAC AGAGGCTGTG CACTCAGGTC TGGCCCGTCA GGTGTCCAGC 
CTTCTCACTA ACCACCTCGC CCGAGCGACG GAGAGCTGCG GCAACCAGGC TGCCGGGAAC 
GATGCCCTCC AGGACGTCCT GAGTCTTCTC AATGACCTCT CGAGGAGTCA CATAGGTAAA 
GCCATCCTGA GCCAGCCAGC TTGTGTGTCC AAACTCCTCT CCCTGCTGCT TGACCAACGC 
CCGTCCCCAA AGCTGGTCCT TATCATTCTT CAGCTGTGCC GGGCAGCACT CCCGCTGATG 
AGCGTAGAAG ACTGTGGGAA TGTGGAGCTC CCACCCTGGA GCTACTCTGT CCCCTCCCTA 
AACAGTGAGC AAGAGGATCC CAGCGACCCA GCGTCCAAGA TCGCCTCTTT GCTCTTAGCA 
AAGCTAGCAG ATTATGTGGT CCCAGGATGT CAGACAGTCC TTTCTCCAAC CGCTTCTGAA 
CCTGACACCA TGTTGACAAA AACCAGCCCC AAGAGTTCCT TAAAAGGAGA TAAAGACCCT 
GGAGAAGAGA GTGAAGCAGT GGATGGCAAG CTCTCTATCT TTATCCACAA GCGGGAAGAC 
CAGTCATCCC ATGAAGTTCT CCAACCATTA CTAAGTAGCT CAGAAGGACG GCCCTTCCGA 
CTTGGTACTG GTGCCAACAT GGAGAAAGTC GTGAAAATGG ACCGAGACAT GACAAAGGGT 
GGCTGCTGTG AGGTGATTAC AGAAGAGGCG GCAGCGGCTC TGCGAAAAGC AACCAAGTGG 
GCACAGTCTG GCCTCATCGT CAGCGTCGGG CCACCCGTGG AATCGCTCAA CCCAGAGACT 
GTGAGTGGAC TGTCCACAGG GGACAAGAAG AAAACCGCCC AAACTTCCAT TTGCCGAGAG 
AGGAACTCTG AACTTGCCAG GACCGACCCT GTCCGACCTT TCATCAGTGG GCATGTGGCA 
AACAGCATGG CTGCAGAGGT GATCGCCTTG CTTCACAGCT TGCTGATGGC GCCTGAGTCA 
AATGCTGCTC AGATATGGAC CACGACAGCA GAGAAGGTTC TGTCTCGTGC GCTGATGTAC 
ATTCCACAGT TGGGGAAATA TGCAGAAAGC ATTCTGGAAA ATGGCAGCAG TAGTGGCAGA 
AAACTCGCCA AACTTCAGAG AATCGCCCGC CAGGCTGTCG CTGCACTGTG TGCACTTGGA 
GGCTTCAAAG AGACAATCAA GATAGGGTCT GAGGTTCAGG TTTTAGGTAG AGGAATATCA 
GGAAGTGTCG GAGTAGTGGC TTCTATCAAT GAACAGGAAG GTATAGCTAC AGTCAAATTC 
CCACCCATAG ACTGTAGAAA GACGTCGCAA GCATCAGACA CACTGACTAT TCCATTGTCT 
AGACTTTGTG TTCCAAGATC AGAGGCATTG CCTCTTCATA AACTGTCCAT TACTGAGAAG 
GTAGTACAGG CAGTCCAGTC CATGTTGCTT CCTCAGGAGG GAAGTCTCTC TATTCATACC 
TCACTTCCTG CAACAGGAGA TGGGTCAGCT CCTGTGATGG CAGTCGTTCG GCTCCTGGCT 
GAAATAAGAA CAAGAGCATG CCTGGTCATG GCCCAGCTTT TAGAAGACAG CTTATTTTGT 
GAAGAATTCA TACAGCAGTG TCCAGCAGCT GTTGAGGTTC TCAACCTAGT AGCCCAGGAA 
TGCAGTGCTG GAGAGCGACT GGCAGTTGTG GAAGTGCAGT GTGAACGGCT GAGGATGCTC 
TACCGGGACT GTGCTCGGCC CCCGCCACCT CCGCTGCAGG CTGACCGGAG ACAGCCCAAA 
GAGATTACGT GGAGCCCCTC ACGAGTGTTT CCACCTGTCC GAGCCTGCAT GTTCTCATCG 
CACCTCACTT CCGTCACCTT CCTGGCTGAC CCCAGCGCTG GGGGCGGTCT GCCCCGGGGC 
ACATTTATCT ATGCCACCTC ACCACTGCCC ATTCAGGCCC CATCTTTCTA CTGGGAAATT 
GAAATTGTTT CCTATGGAGA TAGTGATGAT GACACTGGAC CAATAGTTTC CTTTGGCTTT 
GCTACAGAAG CAGAGAAAAG AGACGGGGCT TGGACTAATC CAGTGGGCAC TTGTCTTTTC 
CATAACAATG GCCGAGCCGT GCACTACAAC GGCTCCAGTT TATTACAGTG GAAGAGCGTT 
CGCTTAGACG TGACTCTCTC TCCTGGTGAC GTGGCAGGAA TTGGCTGGGA GAGAACCGAG 
GGAACGCCTC CTCCTCCAGG GCAGCCAGCC AAGGGCCGAG TGTACTTCAC ATACTGCGGG 
CAGCGCCTCT CACCGTATCT TGAAGATGTT TCGGGTGGGA TGTGGCCTGT GGTTCACATT 
CAGAAAAAGA ACACCAAGAC GCGAGCTAAC TTTGGCTCCC GCCCATTTGC CTATGCTGAA 
GGGCAGGCCC ACCGCAACGC AGCTGACCTG TGCACCGACC TGGCAGAGGA GATCAGCGCT 
AACTTCGAGG CCCTGCCCTT TGCCATGGCA TCTGACAGTG ACAACGATGC TGGCACCAGT 
ATTGCGTCAG ACCCGGGCAC TCACGGGCCA CCGTGCCGGA TTGCTGCCGT GGCCACGGCT 
CAGCAACAAT ATGATAGTGA CACTTCCTGT CATTATAAAG TAGAGCTCAG CTATGAGAAT 
TTCATCACCT CCGGCCCAGA CCCCCATCCG CCCCCCATTG CAGATGACGA AAGTGATGAT 
GATGATGACG ATGATATCCC GCAGGAGGAC CACTATGCTC TACTGGTGAA AGCGTGGGAG 
ACCAAGGTTT TTCCTACTAT CCGAAGACGC TTCCGCAACG AAGCAGAGCG GAAATCGGGC 
CTGGACCAGA TAAAGGGTGC TTTACAACTA GGCATGGTGG ATATTGCCCG GCAGACAGTT 
GAATTCCTCT ACGAGGAGAA CGGTGGCATC CCAAGAGACC TTTATCTTCC CACCATTGAA 
GACATTAAGG ATGAAGCAAA CAAGTTCACA ATTGATAAAG TTCGAAAAGG TCTCACGGTA 
GTAACCCGCT CTCCAGACAG CAATAACGTA GCCAGCAGTA CTGTTGGAAC TGCCCTGCCA 
AAATTTGCCA TCCGAGGGAT GCTGAAAACC TTTGGGCTTC ATGGAGTTGT CTTAGATGTT 
GATTCAGTAA ATGAATTGGT GCAGGTAGAA ACGTACCTCC GAAGCGAAGG TGTGCTGGTC 
CGATACTGGT ATCCTATTGA TATGTTGGAA AGGCCCCCAG CAGGCTACCG AAGGACTGCC 
ACCAATGGGC TGGTCACCCT GGACAATACC AACCTTCAAA TTCACAGGGA GCTGCTGCGC 
TGCGAGGCCG CACTGGCCAG GCTGTACTGC CGCATGGCCC TGCTCAACAT CTTCGCCCCC 
AGGCTGCCTC ACCTGTTTAC CCGCCTCTTC CACATCCCTG CCATCCGGGA CATTACCCTG 
GAGCACCTGC AGCTGCTGTC CAATCAGCTC CTCGCTCCTC CCCTCCCAGA TGGCACCATC 
AGCTCCAGCT CCATCCTCCT GGCACAGTCG CTGCAGCACT GCATCCATTC CCAGAACTGC 
TCGGCCACGG ACCTCTTCTA CCAGGGCAGC TCCCAGACAG TGAGAGAGTG GCTCAACGTG 
GCCATCACTC GGACCCTGCA CCAGGGTGAG GAGAGCCTGT TGGAGCTGAC GAAACAGATC 
TGCTCTTTCC TGCAGACGGC ACCAGAGCAG TTCCCCTCCG AAGAGTTTCC AATTTCCGAG 
TCCAAGGTCA ACATGGACGT CAATTTCCCC GGGGCGGCTT TCGTCGTGGT ATCTTGCAAA 
GAAAGTCAGT CTGGATTCCG CAAAGACTCC TCTCTGTACA AGGCGCCGTG GGCGCGCGTG 
CTCGTGTACG GACTGGGCCA CAAAGTGAAG CGAAATGGCC AGCTAAACCT CATCGAGGCC 
ATCTGTTACC CGCGGGATGC GTCTCCGGCC AACACGGGGC TCACGCCGCC TCCCACCACC 
AACCAGTACC CTTCTGTGAT CCTCTCCACA GACAAAGTCC ACATCAAACT GGGGGTGTCT 
CCCCCTCCTG GAGCCGTGCT GGTGTTACAC TCTCTGCCCC TTGAGTTCCC ACTGGCGATG 
GCCTTCGCAG AGCAGCTGCT GTCCTGGAAG TCGGAGGACG GTGAAGGGAG ATCTGAAGAC 
GAGCCTGACA CCATCCCGAC GTCCGTCCTC CTGCAAGTGG TGGAACTGCT CGGAAACTTC 
CTGTGGACCA CGGACATGGC GGCCTGTGTG AAAGAGCTGG TTTTCCACCT CCTGGCAGAG 
CTCGTGCGCA CAGTGCACAG CCTGGAGCAG AGGAAGCACC CTGCCGGCCT GTCCTCGTCC 
ATCGCCCTCC AGCTGAACCC CTGCCTGGCC ATGCTGATGG CCCTGCAGTC AGAGCTCCAC 
AAGCTGTACG ACGAGGAGAC GCAGAGCTGG GTCTCGGGCA GCACGTGCGG AGGCTCGGGG 
ACCGTGGCGG CCACCGACCA GGGCAGGTTC TCTACGTATT TCCACGCGCT CATGGAAGGG 
TGCCTGGCGG TGGCCGAGGT GACCCTGCCT ACCAACATGA GCGTCACTGC CAGCGGGGTG 
ACGTCGGCGA CCGCCCCAAA CCTCAGTGAC TCGTCGTCCT CCTCGTCATC CTCCCCAGGA 
CAGACGCCGC AGAGCCCCAG CCTCCTGTCC AAGAGGAAGA AAGTCAAGAT GAAGCGGGAA 
AAGGCCTCCT CCTCTGGGAA GCGCCAGTCC TCGCACTCAG TGGACTCGGA CCCCGCCGTG 
CTGAGCATCG GGGGTGGCAA GCCCGAAGAC ATGCTGTGGT TCCATCGCGC CCTGACCTTG 
CTCATCATCC TCCGGCACCT CACCAGGAAG GACCCGCAGG GGCTGGGTGT GACGAGCGAC 
GCCATCGCCG ACGCCTGCCA GGCCCTGGTG GGTCCCACCG CCCACAGCCG CTTGCTGGTG 
ATCTCTGGGA TCCCCACCCA CCTGGACGAG GGCATCGTCA GAGGCGCCAT CCGCAAGGCC 
TGCAACGCCC ACGGCGGGGT CTTCAAAGAC GAGATCTACA TCCCCCTGCA GGACGAAGAC 
CCCAAGAAGC CAAAAGACAA GGCCGAGGGC GGTGATGGGA AAGCCGAGCC AGAGAAGACC 
CTTGGCTTCC CCAGCACAGA CAGCTTGGAG GTCAGCACGT CCCGCAGCCC GACCCCCGCC 
ATGAGCATCA GCGCGTCTGC GTCCACCAGC CAAGCCTCCA TCTGCAGCTC CCAGGGCATC 
TTGCAGACAG TCAGCGATGT CTCTGTGGAA CCGCTGCCCT CGGGCCTCGA GCTGCCCATC 
CCTGCGGGCT TATTGGAGCC CCACGTAGTG TCCAGCCAGG AGAGCCTGGA CGTTTCCCTG 
TGCAGCACCA GCAGCCTGGG CAGCCTCGGG AGCCTGGGGG AGCCACTGGA CAACGCAGAA 
ACGGCCTCAG TGTCAGACAT GGGCTCCATG TACACAGTCA CGTCCCTGGA CAACCAGCCC 
CTCGCAGCAC GCCCCATCAA AGGCTTCGCG GTCGTGGAGA TTAGATCCCG AGCCAAAATC 
GAGAAAATCC GAGCGAGTTT ATTTAACAAT AATGACTTGA TTGGTTTGTC AAGTTTGGAT 
GGTGAAGATG AATTGATGGA AATGTCAACG GAAGAGATCC TCACTGTGTC TGTAGTAAAT 
CAGAGTTTGT TTGACACGCA GGGGAGCCCA GGGTTAGAAG ATTATTTCAA TGATAAGTCA 
ATTAAAGGTG AGAAGCTGGT GCCCGGGGCC AGAGAGGTGC TGACGGAGAT ATTTAAGAGC 
TGCGCCCACT CAGAGCAGAT GCTGAGTCTG ACACCAGCAA AGCCCATCAA AGTGTCTGAC 
ATTTATCTCA GCAAAGAGCA GATCAACTCC CAGACGCCAG GCAACCTCCT CCACCTCTTC 
TTCACCAATG TTCGGCCCCC CAAAAAGGTG CTGGAGGACC AGCTCACCCA GATCCTGAGG 
AAGTACGGCG TGCCAAAACC CAAGTTCGAC AAGAGCAAGT ACAGCAAGGC GGGGAAGGAA 
CAGCACCCCG GGAAGGTGGT GAGCACCAAG CGGCCCATCA CCAAGCCACC TGCCAAGGAC 
AAGGCCGTGC TCAACAGCGT CAGCAGGACT GCCTTGAGCG AGAAGAAGCC CACTGTGAAG 
CCGAAGTCAC CGGAAAAGAG CAAGCCGGAC GAAAAGGACC CGGAAAAGTC ACCCACCAAA 
AAACAAGAAG TCCCTGAGGA AAAGTACCTG ACATTGGAAG GATTTCACAA GTTTGTTATT 
GACCGAGCCA AGCAAGACAT CCGTAGTGTC TGGAGGGCGA TTTTATCCTG TGGTTACGAT 
CTTCATTTTG AGAGGTGTGC CTGCATCGAC GTCCGGCACG CACACAAGGC CTCCAGGAAG 
TGGACCCTGG AGATGGACGT GGCGCTCGTG CAGTACATCA ACCGGCTGTG CCGCCACCTG 
GCCATCACGC CCGCGCGGCT GCATCCCCAC GAGGTGTATC TGGAGACCAC AGACGCCGCC 
GACCCCAGAG TGGCCTGTCT CTTGAATGTG CCCATTGAGA GCCTGCGCCT GCGCTTCGCC 
CTGCTGCAGT CCCTGAACAC CACGCTCGAG ACCTTCTTCC TGCCACTCGT GGAGCTGCGC 
CAGACGCCCA TGTACACGCA CAGCATCGCG GCCCTGCTGA AGGAGGCCAA AGGGCTGATC 
TTTTATGACA CGAAGGTGAC CGTCATGAAT CGGGTGCTGA ACGCCACAGT GCAGAGGACG 
GCTGACCACG CGGCACCGGA GATTACTCTG GACCCACTGG AGATCGTGGG AGGGGAGATC 
AGAGCCTCTG AAAACTCCTA CTTCTGCCAG GCGGCCAGGC AGCTGGCCTC GGTGCCATCC 
TCCCAGCTTT GCGTCAGACT GGCGAGTGGC GGTGACCCCA CCTATGCCTT CAACATCCGC 
TTCACTGGGG AGGAGGTCCA CGGCACCAGC GGCTCCTTCC GCCACTTCCT GTGGCAGGTG 
TGTAAAGAGC TGCAGAGCTC CGCGCTCTCC CTTCTCCTGC TGTGCCCCAG CTCAGCCGTC 
AACAAGAACA AGGGCCAGTA CATCCTGAGC CCAAGCCCCA TCAGCTACGG GGAGGAGCAG 
CTGCTGCACT TCCTGGGCCA GTTGCTGGGA ATTGCGATTC GAGCAGATGT CCCTCTTCCC 
CTGGACCTCC TACCCTCCTT CTGGAAGACG CTGGTGGGCG AGCCCCTGGA CCCTGACCAA 
GACCTGCAGG AAGCAGACAT ACTCACCTAC AATTATGTCA AGAAATTCGA GAGTATCAAC 
GACGAGAGCG AGCTGGAGGC CCTGTGCGCC GAGATTGCCT CCCAGCACCT GGCCATCGAG 
AGCCCCGACG GGCCCAGTAA GCCCTGCTGC CGGTTCACCT ACCTGACCAT GGCGGGCGAG 
GAGGTGGAGC TGTGCAGCCG CGGCCGGCAC ATCCCTGTGG CGTGGGAGAA CAAGGACATC 
TACGCAGCAG CCATCCGGAG CCTGCGGCTG CGGGAGCTGC AGAACGTCGA GTGTGTGACA 
GCCGTGCGCG CGGGCCTCGG CGCCATCATC CCCCTGCAGC TGCTCACCAC GCTCAGCCCG 
CTGGAGATGG AGCTGCGGAC GTGCGGCCTC CCTTACATCA ACCTCGAGTT CCTGAAGGCC 
CACACCATGT ACCAGGTGGG CCTGATGGAG ACGGACCAGC ACATCGAGTT CTTCTGGGGG 
GCACTGGAGA TGTTTGCCCA GGAGGAGCTG TGCAAGTTCA TCAAGTTTGC CTGCAACCAA 
GAGCGCATCC CGTTCACCTG CCCCTGCAAA GACGGGGGCC CCGACACAGC CCACGTGCCC 
CCATACCCCA TGAAGATCGC CCCCCCGGAT GGCACAGCAG GTTCCCCAGA CTCTCGCTAC 
ATCCGAGTGG AGACCTGCAT GTTCATGATC AAGCTTCCCC AGTACTCCTC TCTGGAAATC 
ATGCTGGAGA AACTTCGTTG TGCCATTCAC TATCGCGAAG ACCCCCTGAG TGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023976558.1
CDS	55..13311
Translation	MGSSAAAAAAAAAAADSAQWLSVKEETIFLHDGLIRVTDLAELPSEILGAPEAADTDLEILTFETKNPTELAERLRSVCGNQSNAYARLLEYRLNALRGLWNAQRQLALEEQHERESSGDEETLALLKRQGLLQQPEQAPFTSRMGLLLVFPLIQSQSRTDPSLCNITAEVLLNCLRDCQPLSLTKEPADCLNGIETLLCSWLEETSDTGRHIPHKQKENAAAALVALACARGSLKTFVHTVHLLQKQTDLGSLPVADVLYRLLLLEGGPGSPSCLLGGKHIVSWGYEDMLPAPDSNTGSSSENKDADLGRCVTADGLYLYTTNSVGRGVSKLGSGLHGTLRGFVYCRNEELEPGWIAFGSGSLLHRPVSFDNKPHSLFQVIDQNTLQVCQMVPMPANHLPVGSTMSTVHLSSDGTYFYWIWSPASLNEKTPKGHSVFMDIFELVVENGVFVANPLQERTILMRKEGESAKSINEMLLSRLSRYRASPSATLAALTGSTISNTLKEDQAANTSCGLPLKMLRKTPIYTCGTYLVMLVPPPGGSGSSATRSLFGGTSGLSSLKILASSLVYNISDGQFASRADLIDAAGSSLGRGALVPGLGACYDTVNNMLWTCSNDYIDQWCNPGNQAFHYVCQRLGVSHIITEPKEEAITTNEVINQLLHHVGAMCIHQLNLLATNPNLPITSVLGKQHPIEAHHLSSICDIMEKAMVNGDTCIIRCILVVFQVVFKFFFSPQTERNRDIIRRSGLLLWQLLMAPKDQICPEIQKEVCLAISSGLNILYPGETEINNLLKLVLTEGERNSGLSQLRDVILTNLAEQLQNNRFGSEEDDHYRLNDELLHYILKIVVRESCILITKCQTVSKDDFQKLLSTVPAASSCLRYLMAVQNHLLSNTVLIKPDENDDSDNSLQGETLKVQELKVSILALATQILTGCDEVLEMLQQVTTALINSDIADREQRLKGLEQVTKATMLGHLLPVLLTSLMHPNLQTLTMADALMPQLVQLVLYTSQTALLLKTQCPVFAEAGCSPCGASDQKGRLFPDERMLEEKEEPGFLTGLKIPAPWAAGKTVETVHPVRDNYKFKETVHIPGARCLYLRFDSRCSSQYDYDKLVIYAGPNTNSRKVAEYGGNTLGYGSRSVLGTGWPKDLVKVEGDTVTFSFEMRSGREHNTPDKAMWGFACTVRAQESSEDVSGGLPFLVDLALGLSVLACSMLRILYNGPEITKEEEACQELLRSKLLQRCQWQVEANGVISPALTPSPSPLPLTIEEDREFTYPSDVLVPPVGNYFDLPRIRLPPGIMIKLREISGRARPQFRPSIKEVIQPDVMEEMVVSCVIKHLNLVDALQSLINFQYQEEHAEEYDLLCKIMGETFKKLNAMERQLQSVAELEQKWQSEVDDATQGKLENNMPFFYDYHFNENKMKELELLCSMKEVSFDGNDLENMVLSLREKFLQEVNSLIQKPSHPLAKTKTLVKSLMNRAELLLHVTIAAQSGLTRSISGTPAETPACKSASETKVISHAVRQPVFLRSMSAPSDLEMIGNDDLEFTRANQRRRHVTSHRSSSFTLLQSLAIEDSRDKPTYSVLLGQLFAFIGTNPDQAVSSSSFLLAAQTRWRRGNTRKQALVHMRELLTAAVRVGGVTHLVGPVTMVLQGGPRIEELTCGGMVEQVQEAFGETMTSVVSLCARYPIACANSIGLLCTIPYTRSEEKCLVRSGLVQLMDRLCSLSSQTESSSSEKQTKKQKVATMAWAAFQVLANRCVEWEKEEGGSTEAVHSGLARQVSSLLTNHLARATESCGNQAAGNDALQDVLSLLNDLSRSHIGKAILSQPACVSKLLSLLLDQRPSPKLVLIILQLCRAALPLMSVEDCGNVELPPWSYSVPSLNSEQEDPSDPASKIASLLLAKLADYVVPGCQTVLSPTASEPDTMLTKTSPKSSLKGDKDPGEESEAVDGKLSIFIHKREDQSSHEVLQPLLSSSEGRPFRLGTGANMEKVVKMDRDMTKGGCCEVITEEAAAALRKATKWAQSGLIVSVGPPVESLNPETVSGLSTGDKKKTAQTSICRERNSELARTDPVRPFISGHVANSMAAEVIALLHSLLMAPESNAAQIWTTTAEKVLSRALMYIPQLGKYAESILENGSSSGRKLAKLQRIARQAVAALCALGGFKETIKIGSEVQVLGRGISGSVGVVASINEQEGIATVKFPPIDCRKTSQASDTLTIPLSRLCVPRSEALPLHKLSITEKVVQAVQSMLLPQEGSLSIHTSLPATGDGSAPVMAVVRLLAEIRTRACLVMAQLLEDSLFCEEFIQQCPAAVEVLNLVAQECSAGERLAVVEVQCERLRMLYRDCARPPPPPLQADRRQPKEITWSPSRVFPPVRACMFSSHLTSVTFLADPSAGGGLPRGTFIYATSPLPIQAPSFYWEIEIVSYGDSDDDTGPIVSFGFATEAEKRDGAWTNPVGTCLFHNNGRAVHYNGSSLLQWKSVRLDVTLSPGDVAGIGWERTEGTPPPPGQPAKGRVYFTYCGQRLSPYLEDVSGGMWPVVHIQKKNTKTRANFGSRPFAYAEGQAHRNAADLCTDLAEEISANFEALPFAMASDSDNDAGTSIASDPGTHGPPCRIAAVATAQQQYDSDTSCHYKVELSYENFITSGPDPHPPPIADDESDDDDDDDIPQEDHYALLVKAWETKVFPTIRRRFRNEAERKSGLDQIKGALQLGMVDIARQTVEFLYEENGGIPRDLYLPTIEDIKDEANKFTIDKVRKGLTVVTRSPDSNNVASSTVGTALPKFAIRGMLKTFGLHGVVLDVDSVNELVQVETYLRSEGVLVRYWYPIDMLERPPAGYRRTATNGLVTLDNTNLQIHRELLRCEAALARLYCRMALLNIFAPRLPHLFTRLFHIPAIRDITLEHLQLLSNQLLAPPLPDGTISSSSILLAQSLQHCIHSQNCSATDLFYQGSSQTVREWLNVAITRTLHQGEESLLELTKQICSFLQTAPEQFPSEEFPISESKVNMDVNFPGAAFVVVSCKESQSGFRKDSSLYKAPWARVLVYGLGHKVKRNGQLNLIEAICYPRDASPANTGLTPPPTTNQYPSVILSTDKVHIKLGVSPPPGAVLVLHSLPLEFPLAMAFAEQLLSWKSEDGEGRSEDEPDTIPTSVLLQVVELLGNFLWTTDMAACVKELVFHLLAELVRTVHSLEQRKHPAGLSSSIALQLNPCLAMLMALQSELHKLYDEETQSWVSGSTCGGSGTVAATDQGRFSTYFHALMEGCLAVAEVTLPTNMSVTASGVTSATAPNLSDSSSSSSSSPGQTPQSPSLLSKRKKVKMKREKASSSGKRQSSHSVDSDPAVLSIGGGKPEDMLWFHRALTLLIILRHLTRKDPQGLGVTSDAIADACQALVGPTAHSRLLVISGIPTHLDEGIVRGAIRKACNAHGGVFKDEIYIPLQDEDPKKPKDKAEGGDGKAEPEKTLGFPSTDSLEVSTSRSPTPAMSISASASTSQASICSSQGILQTVSDVSVEPLPSGLELPIPAGLLEPHVVSSQESLDVSLCSTSSLGSLGSLGEPLDNAETASVSDMGSMYTVTSLDNQPLAARPIKGFAVVEIRSRAKIEKIRASLFNNNDLIGLSSLDGEDELMEMSTEEILTVSVVNQSLFDTQGSPGLEDYFNDKSIKGEKLVPGAREVLTEIFKSCAHSEQMLSLTPAKPIKVSDIYLSKEQINSQTPGNLLHLFFTNVRPPKKVLEDQLTQILRKYGVPKPKFDKSKYSKAGKEQHPGKVVSTKRPITKPPAKDKAVLNSVSRTALSEKKPTVKPKSPEKSKPDEKDPEKSPTKKQEVPEEKYLTLEGFHKFVIDRAKQDIRSVWRAILSCGYDLHFERCACIDVRHAHKASRKWTLEMDVALVQYINRLCRHLAITPARLHPHEVYLETTDAADPRVACLLNVPIESLRLRFALLQSLNTTLETFFLPLVELRQTPMYTHSIAALLKEAKGLIFYDTKVTVMNRVLNATVQRTADHAAPEITLDPLEIVGGEIRASENSYFCQAARQLASVPSSQLCVRLASGGDPTYAFNIRFTGEEVHGTSGSFRHFLWQVCKELQSSALSLLLLCPSSAVNKNKGQYILSPSPISYGEEQLLHFLGQLLGIAIRADVPLPLDLLPSFWKTLVGEPLDPDQDLQEADILTYNYVKKFESINDESELEALCAEIASQHLAIESPDGPSKPCCRFTYLTMAGEEVELCSRGRHIPVAWENKDIYAAAIRSLRLRELQNVECVTAVRAGLGAIIPLQLLTTLSPLEMELRTCGLPYINLEFLKAHTMYQVGLMETDQHIEFFWGALEMFAQEELCKFIKFACNQERIPFTCPCKDGGPDTAHVPPYPMKIAPPDGTAGSPDSRYIRVETCMFMIKLPQYSSLEIMLEKLRCAIHYREDPLSG

Target ORF information:

RefSeq Version	XM_024120790.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon HECT domain E3 ubiquitin protein ligase 4 (HECTD4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024120790.1

ORF Insert Sequence:

ATGGGCTCGT CGGCAGCCGC GGCGGCTGCG GCGGCGGCGG CCGCGGACTC GGCGCAGTGG 
CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG 
GCCGAGCTGC CCAGCGAGAT TCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTGGAGATT 
TTAACATTTG AGACCAAGAA TCCGACGGAA TTAGCAGAAC GTTTGCGCTC TGTTTGTGGG 
AATCAGAGCA ATGCGTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG 
TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAAAG TTCGGGTGAC 
GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC 
TTCACGTCGA GGATGGGGCT TCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA 
GACCCCTCTC TCTGTAACAT AACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG 
CCTTTGAGCT TGACCAAGGA GCCTGCTGAC TGTCTGAATG GAATCGAAAC TTTGCTGTGC 
TCTTGGCTAG AGGAGACTTC TGACACAGGC CGACACATCC CACATAAGCA AAAAGAAAAT 
GCTGCTGCTG CCCTGGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT 
ACAGTCCATC TATTACAGAA ACAGACAGAT CTAGGGTCCC TGCCTGTAGC CGATGTGCTG 
TATAGGCTGT TGCTTTTGGA AGGGGGACCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA 
CACATAGTAT CATGGGGGTA TGAAGACATG TTGCCTGCAC CAGATAGCAA CACTGGCTCC 
AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTGTCA CGGCAGATGG TCTTTACCTG 
TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT 
CTCAGAGGTT TTGTGTACTG CCGGAATGAG GAGCTGGAAC CAGGATGGAT TGCTTTTGGC 
AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC TCTTTTCCAG 
GTCATTGACC AGAATACCCT TCAGGTGTGC CAGATGGTGC CAATGCCAGC CAATCACCTC 
CCTGTGGGCA GCACTATGAG CACCGTGCAC CTTTCTTCAG ATGGTACTTA CTTCTATTGG 
ATCTGGTCTC CTGCCAGTCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC 
ATTTTTGAAC TTGTGGTTGA AAATGGTGTA TTTGTAGCCA ACCCGCTTCA GGAACGCACA 
ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT TCTGAGCAGA 
CTTTCACGCT ACCGAGCCTC GCCATCCGCA ACGCTGGCTG CCCTTACCGG CTCCACCATC 
TCCAACACCC TGAAAGAGGA CCAGGCAGCA AATACTAGCT GTGGGCTTCC TCTAAAAATG 
CTTCGGAAGA CGCCCATTTA CACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA 
GGGGGATCAG GCAGCTCTGC TACCCGGTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT 
TTAAAAATCC TAGCTTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CGCAAGTCGT 
GCTGATCTCA TAGATGCTGC TGGAAGCTCA TTGGGGCGTG GTGCTCTTGT ACCAGGATTA 
GGTGCCTGTT ACGATACAGT GAACAACATG CTATGGACAT GCTCGAATGA CTATATCGAT 
CAATGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GTCAGAGACT TGGAGTCAGC 
CATATCATCA CTGAGCCTAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA 
TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT 
CTTCCAATCA CAAGTGTCTT GGGCAAGCAG CATCCAATTG AAGCACACCA TCTTAGCAGT 
ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATCAT ACGCTGCATT 
CTCGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA 
GACATCATTC GACGGTCGGG GTTGCTTCTT TGGCAGTTGT TGATGGCACC AAAAGATCAA 
ATTTGTCCTG AAATTCAGAA GGAAGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG 
TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA 
AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTAACCA ATTTAGCTGA ACAGCTTCAA 
AACAACCGAT TTGGCAGTGA GGAGGATGAT CATTACAGAC TAAATGACGA ACTTTTACAC 
TACATTTTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC 
TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CTGCATCCTC CTGCCTGCGC 
TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTG TTTTGATTAA ACCTGATGAG 
AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGTACAGGA GCTAAAGGTC 
AGTATTTTGG CTCTTGCCAC CCAAATCCTG ACTGGATGTG ATGAAGTGTT GGAAATGCTA 
CAGCAGGTCA CAACTGCCCT CATAAATAGT GACATAGCTG ACCGGGAGCA GAGGTTAAAA 
GGCTTGGAAC AAGTTACTAA GGCTACTATG CTCGGTCACC TTCTTCCTGT GTTACTGACC 
TCCCTGATGC ATCCAAATTT ACAGACTCTG ACCATGGCCG ATGCCCTGAT GCCTCAGCTA 
GTGCAGCTGG TACTTTATAC CAGTCAGACG GCTTTGCTGC TCAAAACCCA GTGTCCGGTT 
TTTGCTGAGG CGGGCTGTTC CCCATGTGGT GCATCAGACC AGAAGGGCAG GCTGTTCCCT 
GATGAGAGAA TGCTGGAAGA GAAGGAAGAG CCAGGGTTTC TCACTGGTTT AAAGATTCCT 
GCTCCATGGG CTGCTGGGAA GACTGTGGAA ACAGTCCATC CTGTCAGAGA CAACTATAAA 
TTTAAAGAAA CTGTCCATAT CCCGGGAGCT CGCTGCCTAT ACCTTAGATT TGATAGCAGA 
TGCTCTTCAC AATATGACTA TGACAAATTG GTGATATACG CGGGGCCTAA CACAAACAGT 
AGGAAGGTTG CTGAGTATGG AGGCAACACT CTGGGATATG GCAGCCGTAG TGTCTTAGGA 
ACTGGTTGGC CGAAAGACTT GGTGAAGGTG GAAGGAGATA CAGTCACCTT CTCCTTTGAA 
ATGAGAAGTG GCCGTGAACA CAACACTCCC GATAAAGCTA TGTGGGGCTT TGCTTGCACA 
GTTCGCGCTC AGGAGTCTTC AGAGGATGTG TCGGGAGGCT TACCTTTTCT GGTAGACCTG 
GCTTTAGGTC TGTCTGTGTT AGCTTGTTCT ATGTTAAGAA TCCTATACAA CGGACCAGAA 
ATTACCAAAG AAGAAGAAGC CTGTCAGGAG CTGCTGCGGT CCAAACTTTT ACAAAGGTGC 
CAGTGGCAGG TGGAGGCCAA TGGTGTGATC TCCCCTGCCC TTACTCCAAG TCCGTCTCCA 
CTGCCTCTGA CCATCGAGGA AGACAGAGAA TTCACTTACC CCTCTGACGT CCTCGTGCCT 
CCTGTTGGAA ACTACTTTGA CCTGCCCCGG ATCAGGCTGC CTCCAGGAAT CATGATAAAG 
CTCAGGGAAA TTTCTGGGCG TGCCAGACCT CAATTTAGAC CAAGTATAAA AGAAGTAATC 
CAGCCAGACG TGATGGAGGA AATGGTGGTA TCGTGTGTCA TTAAGCACTT GAACTTGGTT 
GATGCACTGC AGTCCCTAAT AAATTTCCAG TATCAAGAAG AACATGCTGA GGAATATGAT 
TTATTGTGTA AAATCATGGG AGAGACATTT AAGAAACTCA ATGCCATGGA GAGACAGCTG 
CAGAGTGTTG CAGAATTGGA ACAGAAATGG CAAAGTGAGG TTGATGATGC CACGCAGGGG 
AAACTGGAGA ACAACATGCC TTTCTTCTAT GATTACCATT TTAATGAGAA CAAAATGAAA 
GAACTAGAAC TTTTGTGTTC AATGAAGGAA GTCTCCTTTG ATGGAAATGA TCTTGAAAAC 
ATGGTCCTAT CTCTGAGGGA GAAGTTCCTA CAAGAAGTGA ATTCTCTCAT TCAGAAACCC 
TCGCACCCAC TGGCTAAGAC GAAGACGTTA GTGAAGAGTC TAATGAACCG AGCTGAGCTG 
TTGCTGCACG TCACCATTGC GGCCCAGTCC GGCCTCACAA GAAGCATCTC AGGGACCCCC 
GCAGAAACAC CGGCTTGTAA ATCAGCCTCT GAAACAAAGG TGATATCTCA TGCTGTGAGG 
CAGCCTGTTT TTCTTCGCAG CATGTCGGCT CCTTCTGACC TGGAAATGAT TGGTAACGAT 
GATTTGGAAT TTACTAGAGC AAATCAGAGG CGGCGCCACG TGACCAGCCA CCGCAGCAGC 
TCCTTCACGC TCCTGCAGTC ATTGGCCATC GAGGACAGCA GGGACAAGCC CACCTACAGC 
GTCCTGCTGG GGCAGCTGTT TGCTTTCATC GGCACCAACC CTGACCAAGC TGTGTCCAGC 
AGCAGCTTTC TTTTGGCTGC GCAGACAAGG TGGCGACGGG GAAACACTCG CAAGCAGGCA 
CTGGTGCACA TGCGGGAATT GCTGACGGCT GCCGTGCGAG TCGGGGGAGT GACACATCTT 
GTGGGTCCGG TGACTATGGT CCTTCAGGGA GGACCCAGAA TTGAAGAACT AACCTGTGGT 
GGGATGGTCG AGCAGGTCCA GGAAGCCTTC GGTGAGACCA TGACCTCTGT TGTGTCTTTG 
TGCGCCCGTT ACCCTATTGC TTGTGCGAAT AGCATCGGAC TCTTATGTAC TATACCTTAC 
ACCAGGAGTG AAGAGAAGTG CCTGGTGCGC AGTGGCCTTG TTCAGCTCAT GGATCGACTG 
TGTAGCTTGA GCAGTCAGAC TGAGTCCAGC TCCAGTGAGA AACAAACCAA GAAGCAGAAG 
GTGGCTACCA TGGCCTGGGC AGCCTTCCAG GTGCTTGCCA ACCGCTGCGT TGAATGGGAA 
AAAGAAGAAG GCGGCTCCAC AGAGGCTGTG CACTCAGGTC TGGCCCGTCA GGTGTCCAGC 
CTTCTCACTA ACCACCTCGC CCGAGCGACG GAGAGCTGCG GCAACCAGGC TGCCGGGAAC 
GATGCCCTCC AGGACGTCCT GAGTCTTCTC AATGACCTCT CGAGGAGTCA CATAGGTAAA 
GCCATCCTGA GCCAGCCAGC TTGTGTGTCC AAACTCCTCT CCCTGCTGCT TGACCAACGC 
CCGTCCCCAA AGCTGGTCCT TATCATTCTT CAGCTGTGCC GGGCAGCACT CCCGCTGATG 
AGCGTAGAAG ACTGTGGGAA TGTGGAGCTC CCACCCTGGA GCTACTCTGT CCCCTCCCTA 
AACAGTGAGC AAGAGGATCC CAGCGACCCA GCGTCCAAGA TCGCCTCTTT GCTCTTAGCA 
AAGCTAGCAG ATTATGTGGT CCCAGGATGT CAGACAGTCC TTTCTCCAAC CGCTTCTGAA 
CCTGACACCA TGTTGACAAA AACCAGCCCC AAGAGTTCCT TAAAAGGAGA TAAAGACCCT 
GGAGAAGAGA GTGAAGCAGT GGATGGCAAG CTCTCTATCT TTATCCACAA GCGGGAAGAC 
CAGTCATCCC ATGAAGTTCT CCAACCATTA CTAAGTAGCT CAGAAGGACG GCCCTTCCGA 
CTTGGTACTG GTGCCAACAT GGAGAAAGTC GTGAAAATGG ACCGAGACAT GACAAAGGGT 
GGCTGCTGTG AGGTGATTAC AGAAGAGGCG GCAGCGGCTC TGCGAAAAGC AACCAAGTGG 
GCACAGTCTG GCCTCATCGT CAGCGTCGGG CCACCCGTGG AATCGCTCAA CCCAGAGACT 
GTGAGTGGAC TGTCCACAGG GGACAAGAAG AAAACCGCCC AAACTTCCAT TTGCCGAGAG 
AGGAACTCTG AACTTGCCAG GACCGACCCT GTCCGACCTT TCATCAGTGG GCATGTGGCA 
AACAGCATGG CTGCAGAGGT GATCGCCTTG CTTCACAGCT TGCTGATGGC GCCTGAGTCA 
AATGCTGCTC AGATATGGAC CACGACAGCA GAGAAGGTTC TGTCTCGTGC GCTGATGTAC 
ATTCCACAGT TGGGGAAATA TGCAGAAAGC ATTCTGGAAA ATGGCAGCAG TAGTGGCAGA 
AAACTCGCCA AACTTCAGAG AATCGCCCGC CAGGCTGTCG CTGCACTGTG TGCACTTGGA 
GGCTTCAAAG AGACAATCAA GATAGGGTCT GAGGTTCAGG TTTTAGGTAG AGGAATATCA 
GGAAGTGTCG GAGTAGTGGC TTCTATCAAT GAACAGGAAG GTATAGCTAC AGTCAAATTC 
CCACCCATAG ACTGTAGAAA GACGTCGCAA GCATCAGACA CACTGACTAT TCCATTGTCT 
AGACTTTGTG TTCCAAGATC AGAGGCATTG CCTCTTCATA AACTGTCCAT TACTGAGAAG 
GTAGTACAGG CAGTCCAGTC CATGTTGCTT CCTCAGGAGG GAAGTCTCTC TATTCATACC 
TCACTTCCTG CAACAGGAGA TGGGTCAGCT CCTGTGATGG CAGTCGTTCG GCTCCTGGCT 
GAAATAAGAA CAAGAGCATG CCTGGTCATG GCCCAGCTTT TAGAAGACAG CTTATTTTGT 
GAAGAATTCA TACAGCAGTG TCCAGCAGCT GTTGAGGTTC TCAACCTAGT AGCCCAGGAA 
TGCAGTGCTG GAGAGCGACT GGCAGTTGTG GAAGTGCAGT GTGAACGGCT GAGGATGCTC 
TACCGGGACT GTGCTCGGCC CCCGCCACCT CCGCTGCAGG CTGACCGGAG ACAGCCCAAA 
GAGATTACGT GGAGCCCCTC ACGAGTGTTT CCACCTGTCC GAGCCTGCAT GTTCTCATCG 
CACCTCACTT CCGTCACCTT CCTGGCTGAC CCCAGCGCTG GGGGCGGTCT GCCCCGGGGC 
ACATTTATCT ATGCCACCTC ACCACTGCCC ATTCAGGCCC CATCTTTCTA CTGGGAAATT 
GAAATTGTTT CCTATGGAGA TAGTGATGAT GACACTGGAC CAATAGTTTC CTTTGGCTTT 
GCTACAGAAG CAGAGAAAAG AGACGGGGCT TGGACTAATC CAGTGGGCAC TTGTCTTTTC 
CATAACAATG GCCGAGCCGT GCACTACAAC GGCTCCAGTT TATTACAGTG GAAGAGCGTT 
CGCTTAGACG TGACTCTCTC TCCTGGTGAC GTGGCAGGAA TTGGCTGGGA GAGAACCGAG 
GGAACGCCTC CTCCTCCAGG GCAGCCAGCC AAGGGCCGAG TGTACTTCAC ATACTGCGGG 
CAGCGCCTCT CACCGTATCT TGAAGATGTT TCGGGTGGGA TGTGGCCTGT GGTTCACATT 
CAGAAAAAGA ACACCAAGAC GCGAGCTAAC TTTGGCTCCC GCCCATTTGC CTATGCTGAA 
GGGCAGGCCC ACCGCAACGC AGCTGACCTG TGCACCGACC TGGCAGAGGA GATCAGCGCT 
AACTTCGAGG CCCTGCCCTT TGCCATGGCA TCTGACAGTG ACAACGATGC TGGCACCAGT 
ATTGCGTCAG ACCCGGGCAC TCACGGGCCA CCGTGCCGGA TTGCTGCCGT GGCCACGGCT 
CAGCAACAAT ATGATAGTGA CACTTCCTGT CATTATAAAG TAGAGCTCAG CTATGAGAAT 
TTCATCACCT CCGGCCCAGA CCCCCATCCG CCCCCCATTG CAGATGACGA AAGTGATGAT 
GATGATGACG ATGATATCCC GCAGGAGGAC CACTATGCTC TACTGGTGAA AGCGTGGGAG 
ACCAAGGTTT TTCCTACTAT CCGAAGACGC TTCCGCAACG AAGCAGAGCG GAAATCGGGC 
CTGGACCAGA TAAAGGGTGC TTTACAACTA GGCATGGTGG ATATTGCCCG GCAGACAGTT 
GAATTCCTCT ACGAGGAGAA CGGTGGCATC CCAAGAGACC TTTATCTTCC CACCATTGAA 
GACATTAAGG ATGAAGCAAA CAAGTTCACA ATTGATAAAG TTCGAAAAGG TCTCACGGTA 
GTAACCCGCT CTCCAGACAG CAATAACGTA GCCAGCAGTA CTGTTGGAAC TGCCCTGCCA 
AAATTTGCCA TCCGAGGGAT GCTGAAAACC TTTGGGCTTC ATGGAGTTGT CTTAGATGTT 
GATTCAGTAA ATGAATTGGT GCAGGTAGAA ACGTACCTCC GAAGCGAAGG TGTGCTGGTC 
CGATACTGGT ATCCTATTGA TATGTTGGAA AGGCCCCCAG CAGGCTACCG AAGGACTGCC 
ACCAATGGGC TGGTCACCCT GGACAATACC AACCTTCAAA TTCACAGGGA GCTGCTGCGC 
TGCGAGGCCG CACTGGCCAG GCTGTACTGC CGCATGGCCC TGCTCAACAT CTTCGCCCCC 
AGGCTGCCTC ACCTGTTTAC CCGCCTCTTC CACATCCCTG CCATCCGGGA CATTACCCTG 
GAGCACCTGC AGCTGCTGTC CAATCAGCTC CTCGCTCCTC CCCTCCCAGA TGGCACCATC 
AGCTCCAGCT CCATCCTCCT GGCACAGTCG CTGCAGCACT GCATCCATTC CCAGAACTGC 
TCGGCCACGG ACCTCTTCTA CCAGGGCAGC TCCCAGACAG TGAGAGAGTG GCTCAACGTG 
GCCATCACTC GGACCCTGCA CCAGGGTGAG GAGAGCCTGT TGGAGCTGAC GAAACAGATC 
TGCTCTTTCC TGCAGACGGC ACCAGAGCAG TTCCCCTCCG AAGAGTTTCC AATTTCCGAG 
TCCAAGGTCA ACATGGACGT CAATTTCCCC GGGGCGGCTT TCGTCGTGGT ATCTTGCAAA 
GAAAGTCAGT CTGGATTCCG CAAAGACTCC TCTCTGTACA AGGCGCCGTG GGCGCGCGTG 
CTCGTGTACG GACTGGGCCA CAAAGTGAAG CGAAATGGCC AGCTAAACCT CATCGAGGCC 
ATCTGTTACC CGCGGGATGC GTCTCCGGCC AACACGGGGC TCACGCCGCC TCCCACCACC 
AACCAGTACC CTTCTGTGAT CCTCTCCACA GACAAAGTCC ACATCAAACT GGGGGTGTCT 
CCCCCTCCTG GAGCCGTGCT GGTGTTACAC TCTCTGCCCC TTGAGTTCCC ACTGGCGATG 
GCCTTCGCAG AGCAGCTGCT GTCCTGGAAG TCGGAGGACG GTGAAGGGAG ATCTGAAGAC 
GAGCCTGACA CCATCCCGAC GTCCGTCCTC CTGCAAGTGG TGGAACTGCT CGGAAACTTC 
CTGTGGACCA CGGACATGGC GGCCTGTGTG AAAGAGCTGG TTTTCCACCT CCTGGCAGAG 
CTCGTGCGCA CAGTGCACAG CCTGGAGCAG AGGAAGCACC CTGCCGGCCT GTCCTCGTCC 
ATCGCCCTCC AGCTGAACCC CTGCCTGGCC ATGCTGATGG CCCTGCAGTC AGAGCTCCAC 
AAGCTGTACG ACGAGGAGAC GCAGAGCTGG GTCTCGGGCA GCACGTGCGG AGGCTCGGGG 
ACCGTGGCGG CCACCGACCA GGGCAGGTTC TCTACGTATT TCCACGCGCT CATGGAAGGG 
TGCCTGGCGG TGGCCGAGGT GACCCTGCCT ACCAACATGA GCGTCACTGC CAGCGGGGTG 
ACGTCGGCGA CCGCCCCAAA CCTCAGTGAC TCGTCGTCCT CCTCGTCATC CTCCCCAGGA 
CAGACGCCGC AGAGCCCCAG CCTCCTGTCC AAGAGGAAGA AAGTCAAGAT GAAGCGGGAA 
AAGGCCTCCT CCTCTGGGAA GCGCCAGTCC TCGCACTCAG TGGACTCGGA CCCCGCCGTG 
CTGAGCATCG GGGGTGGCAA GCCCGAAGAC ATGCTGTGGT TCCATCGCGC CCTGACCTTG 
CTCATCATCC TCCGGCACCT CACCAGGAAG GACCCGCAGG GGCTGGGTGT GACGAGCGAC 
GCCATCGCCG ACGCCTGCCA GGCCCTGGTG GGTCCCACCG CCCACAGCCG CTTGCTGGTG 
ATCTCTGGGA TCCCCACCCA CCTGGACGAG GGCATCGTCA GAGGCGCCAT CCGCAAGGCC 
TGCAACGCCC ACGGCGGGGT CTTCAAAGAC GAGATCTACA TCCCCCTGCA GGACGAAGAC 
CCCAAGAAGC CAAAAGACAA GGCCGAGGGC GGTGATGGGA AAGCCGAGCC AGAGAAGACC 
CTTGGCTTCC CCAGCACAGA CAGCTTGGAG GTCAGCACGT CCCGCAGCCC GACCCCCGCC 
ATGAGCATCA GCGCGTCTGC GTCCACCAGC CAAGCCTCCA TCTGCAGCTC CCAGGGCATC 
TTGCAGACAG TCAGCGATGT CTCTGTGGAA CCGCTGCCCT CGGGCCTCGA GCTGCCCATC 
CCTGCGGGCT TATTGGAGCC CCACGTAGTG TCCAGCCAGG AGAGCCTGGA CGTTTCCCTG 
TGCAGCACCA GCAGCCTGGG CAGCCTCGGG AGCCTGGGGG AGCCACTGGA CAACGCAGAA 
ACGGCCTCAG TGTCAGACAT GGGCTCCATG TACACAGTCA CGTCCCTGGA CAACCAGCCC 
CTCGCAGCAC GCCCCATCAA AGGCTTCGCG GTCGTGGAGA TTAGATCCCG AGCCAAAATC 
GAGAAAATCC GAGCGAGTTT ATTTAACAAT AATGACTTGA TTGGTTTGTC AAGTTTGGAT 
GGTGAAGATG AATTGATGGA AATGTCAACG GAAGAGATCC TCACTGTGTC TGTAGTAAAT 
CAGAGTTTGT TTGACACGCA GGGGAGCCCA GGGTTAGAAG ATTATTTCAA TGATAAGTCA 
ATTAAAGGTG AGAAGCTGGT GCCCGGGGCC AGAGAGGTGC TGACGGAGAT ATTTAAGAGC 
TGCGCCCACT CAGAGCAGAT GCTGAGTCTG ACACCAGCAA AGCCCATCAA AGTGTCTGAC 
ATTTATCTCA GCAAAGAGCA GATCAACTCC CAGACGCCAG GCAACCTCCT CCACCTCTTC 
TTCACCAATG TTCGGCCCCC CAAAAAGGTG CTGGAGGACC AGCTCACCCA GATCCTGAGG 
AAGTACGGCG TGCCAAAACC CAAGTTCGAC AAGAGCAAGT ACAGCAAGGC GGGGAAGGAA 
CAGCACCCCG GGAAGGTGGT GAGCACCAAG CGGCCCATCA CCAAGCCACC TGCCAAGGAC 
AAGGCCGTGC TCAACAGCGT CAGCAGGACT GCCTTGAGCG AGAAGAAGCC CACTGTGAAG 
CCGAAGTCAC CGGAAAAGAG CAAGCCGGAC GAAAAGGACC CGGAAAAGTC ACCCACCAAA 
AAACAAGAAG TCCCTGAGGA AAAGTACCTG ACATTGGAAG GATTTCACAA GTTTGTTATT 
GACCGAGCCA AGCAAGACAT CCGTAGTGTC TGGAGGGCGA TTTTATCCTG TGGTTACGAT 
CTTCATTTTG AGAGGTGTGC CTGCATCGAC GTCCGGCACG CACACAAGGC CTCCAGGAAG 
TGGACCCTGG AGATGGACGT GGCGCTCGTG CAGTACATCA ACCGGCTGTG CCGCCACCTG 
GCCATCACGC CCGCGCGGCT GCATCCCCAC GAGGTGTATC TGGAGACCAC AGACGCCGCC 
GACCCCAGAG TGGCCTGTCT CTTGAATGTG CCCATTGAGA GCCTGCGCCT GCGCTTCGCC 
CTGCTGCAGT CCCTGAACAC CACGCTCGAG ACCTTCTTCC TGCCACTCGT GGAGCTGCGC 
CAGACGCCCA TGTACACGCA CAGCATCGCG GCCCTGCTGA AGGAGGCCAA AGGGCTGATC 
TTTTATGACA CGAAGGTGAC CGTCATGAAT CGGGTGCTGA ACGCCACAGT GCAGAGGACG 
GCTGACCACG CGGCACCGGA GATTACTCTG GACCCACTGG AGATCGTGGG AGGGGAGATC 
AGAGCCTCTG AAAACTCCTA CTTCTGCCAG GCGGCCAGGC AGCTGGCCTC GGTGCCATCC 
TCCCAGCTTT GCGTCAGACT GGCGAGTGGC GGTGACCCCA CCTATGCCTT CAACATCCGC 
TTCACTGGGG AGGAGGTCCA CGGCACCAGC GGCTCCTTCC GCCACTTCCT GTGGCAGGTG 
TGTAAAGAGC TGCAGAGCTC CGCGCTCTCC CTTCTCCTGC TGTGCCCCAG CTCAGCCGTC 
AACAAGAACA AGGGCCAGTA CATCCTGAGC CCAAGCCCCA TCAGCTACGG GGAGGAGCAG 
CTGCTGCACT TCCTGGGCCA GTTGCTGGGA ATTGCGATTC GAGCAGATGT CCCTCTTCCC 
CTGGACCTCC TACCCTCCTT CTGGAAGACG CTGGTGGGCG AGCCCCTGGA CCCTGACCAA 
GACCTGCAGG AAGCAGACAT ACTCACCTAC AATTATGTCA AGAAATTCGA GAGTATCAAC 
GACGAGAGCG AGCTGGAGGC CCTGTGCGCC GAGATTGCCT CCCAGCACCT GGCCATCGAG 
AGCCCCGACG GGCCCAGTAA GCCCTGCTGC CGGTTCACCT ACCTGACCAT GGCGGGCGAG 
GAGGTGGAGC TGTGCAGCCG CGGCCGGCAC ATCCCTGTGG CGTGGGAGAA CAAGGACATC 
TACGCAGCAG CCATCCGGAG CCTGCGGCTG CGGGAGCTGC AGAACGTCGA GTGTGTGACA 
GCCGTGCGCG CGGGCCTCGG CGCCATCATC CCCCTGCAGC TGCTCACCAC GCTCAGCCCG 
CTGGAGATGG AGCTGCGGAC GTGCGGCCTC CCTTACATCA ACCTCGAGTT CCTGAAGGCC 
CACACCATGT ACCAGGTGGG CCTGATGGAG ACGGACCAGC ACATCGAGTT CTTCTGGGGG 
GCACTGGAGA TGTTTGCCCA GGAGGAGCTG TGCAAGTTCA TCAAGTTTGC CTGCAACCAA 
GAGCGCATCC CGTTCACCTG CCCCTGCAAA GACGGGGGCC CCGACACAGC CCACGTGCCC 
CCATACCCCA TGAAGATCGC CCCCCCGGAT GGCACAGCAG GTTCCCCAGA CTCTCGCTAC 
ATCCGAGTGG AGACCTGCAT GTTCATGATC AAGCTTCCCC AGTACTCCTC TCTGGAAATC 
ATGCTGGAGA AACTTCGTTG TGCCATTCAC TATCGCGAAG ACCCCCTGAG TGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD4 ( XM_028480292.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_028336093.1 Physeter catodon HECT domain E3 ubiquitin protein ligase 4 (HECTD4), mRNA.

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CloneID	OPh325006
Clone ID Related Accession (Same CDS sequence)	XM_028480292.1
Accession Version	XM_028480292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	probable E3 ubiquitin-protein ligase HECTD4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041232.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCGT CGGCAGCCGC GGCGGCTGCG GCGGCGGCGG CCGCGGACTC GGCGCAGTGG 
CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG 
GCCGAGCTGC CCAGCGAGAT TCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTGGAGATT 
TTAACATTTG AGACCAAGAA TCCGACGGAA TTAGCAGAAC GTTTGCGCTC TGTTTGTGGG 
AATCAGAGCA ATGCGTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG 
TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAAAG TTCGGGTGAC 
GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC 
TTCACGTCGA GGATGGGGCT TCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA 
GACCCCTCTC TCTGTAACAT AACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG 
CCTTTGAGCT TGACCAAGGA GCCTGCTGAC TGTCTGAATG GAATCGAAAC TTTGCTGTGC 
TCTTGGCTAG AGGAGACTTC TGACACAGGC CGACACATCC CACATAAGCA AAAAGAAAAT 
GCTGCTGCTG CCCTGGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT 
ACAGTCCATC TATTACAGAA ACAGACAGAT CTAGGGTCCC TGCCTGTAGC CGATGTGCTG 
TATAGGCTGT TGCTTTTGGA AGGGGGACCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA 
CACATAGTAT CATGGGGGTA TGAAGACATG TTGCCTGCAC CAGATAGCAA CACTGGCTCC 
AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTGTCA CGGCAGATGG TCTTTACCTG 
TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT 
CTCAGAGGTT TTGTGTACTG CCGGAATGAG GAGCTGGAAC CAGGATGGAT TGCTTTTGGC 
AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC TCTTTTCCAG 
GTCATTGACC AGAATACCCT TCAGGTGTGC CAGATGGTGC CAATGCCAGC CAATCACCTC 
CCTGTGGGCA GCACTATGAG CACCGTGCAC CTTTCTTCAG ATGGTACTTA CTTCTATTGG 
ATCTGGTCTC CTGCCAGTCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC 
ATTTTTGAAC TTGTGGTTGA AAATGGTGTA TTTGTAGCCA ACCCGCTTCA GGAACGCACA 
ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT TCTGAGCAGA 
CTTTCACGCT ACCGAGCCTC GCCATCCGCA ACGCTGGCTG CCCTTACCGG CTCCACCATC 
TCCAACACCC TGAAAGAGGA CCAGGCAGCA AATACTAGCT GTGGGCTTCC TCTAAAAATG 
CTTCGGAAGA CGCCCATTTA CACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA 
GGGGGATCAG GCAGCTCTGC TACCCGGTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT 
TTAAAAATCC TAGCTTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CGCAAGTCGT 
GCTGATCTCA TAGATGCTGC TGGAAGCTCA TTGGGGCGTG GTGCTCTTGT ACCAGGATTA 
GGTGCCTGTT ACGATACAGT GAACAACATG CTATGGACAT GCTCGAATGA CTATATCGAT 
CAATGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GTCAGAGACT TGGAGTCAGC 
CATATCATCA CTGAGCCTAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA 
TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT 
CTTCCAATCA CAAGTGTCTT GGGCAAGCAG CATCCAATTG AAGCACACCA TCTTAGCAGT 
ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATCAT ACGCTGCATT 
CTCGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA 
GACATCATTC GACGGTCGGG GTTGCTTCTT TGGCAGTTGT TGATGGCACC AAAAGATCAA 
ATTTGTCCTG AAATTCAGAA GGAAGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG 
TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA 
AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTAACCA ATTTAGCTGA ACAGCTTCAA 
AACAACCGAT TTGGCAGTGA GGAGGATGAT CATTACAGAC TAAATGACGA ACTTTTACAC 
TACATTTTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC 
TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CTGCATCCTC CTGCCTGCGC 
TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTG TTTTGATTAA ACCTGATGAG 
AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGTACAGGA GCTAAAGGTC 
AGTATTTTGG CTCTTGCCAC CCAAATCCTG ACTGGATGTG ATGAAGTGTT GGAAATGCTA 
CAGCAGGTCA CAACTGCCCT CATAAATAGT GACATAGCTG ACCGGGAGCA GAGGTTAAAA 
GGCTTGGAAC AAGTTACTAA GGCTACTATG CTCGGTCACC TTCTTCCTGT GTTACTGACC 
TCCCTGATGC ATCCAAATTT ACAGACTCTG ACCATGGCCG ATGCCCTGAT GCCTCAGCTA 
GTGCAGCTGG TACTTTATAC CAGTCAGACG GCTTTGCTGC TCAAAACCCA GTGTCCGGTT 
TTTGCTGAGG CGGGCTGTTC CCCATGTGGT GCATCAGACC AGAAGGGCAG GCTGTTCCCT 
GATGAGAGAA TGCTGGAAGA GAAGGAAGAG CCAGGGTTTC TCACTGGTTT AAAGATTCCT 
GCTCCATGGG CTGCTGGGAA GACTGTGGAA ACAGTCCATC CTGTCAGAGA CAACTATAAA 
TTTAAAGAAA CTGTCCATAT CCCGGGAGCT CGCTGCCTAT ACCTTAGATT TGATAGCAGA 
TGCTCTTCAC AATATGACTA TGACAAATCT AACAGTATAT TTTCTGTTTT GAAGTTGGTG 
ATATACGCGG GGCCTAACAC AAACAGTAGG AAGGTTGCTG AGTATGGAGG CAACACTCTG 
GGATATGGCA GCCGTAGTGT CTTAGGAACT GGTTGGCCGA AAGACTTGGT GAAGGTGGAA 
GGAGATACAG TCACCTTCTC CTTTGAAATG AGAAGTGGCC GTGAACACAA CACTCCCGAT 
AAAGCTATGT GGGGCTTTGC TTGCACAGTT CGCGCTCAGG AGTCTTCAGA GGATGTGTCG 
GGAGGCTTAC CTTTTCTGGT AGACCTGGCT TTAGGTCTGT CTGTGTTAGC TTGTTCTATG 
TTAAGAATCC TATACAACGG ACCAGAAATT ACCAAAGAAG AAGAAGCCTG TCAGGAGCTG 
CTGCGGTCCA AACTTTTACA AAGGTGCCAG TGGCAGGTGG AGGCCAATGG TGTGATCTCC 
CCTGCCCTTA CTCCAAGTCC GTCTCCACTG CCTCTGACCA TCGAGGAAGA CAGAGAATTC 
ACTTACCCCT CTGACGTCCT CGTGCCTCCT GTTGGAAACT ACTTTGACCT GCCCCGGATC 
AGGCTGCCTC CAGGAATCAT GATAAAGCTC AGGGAAATTT CTGGGCGTGC CAGACCTCAA 
TTTAGACCAA GTATAAAAGA AGTAATCCAG CCAGACGTGA TGGAGGAAAT GGTGGTATCG 
TGTGTCATTA AGCACTTGAA CTTGGTTGAT GCACTGCAGT CCCTAATAAA TTTCCAGTAT 
CAAGAAGAAC ATGCTGAGGA ATATGATTTA TTGTGTAAAA TCATGGGAGA GACATTTAAG 
AAACTCAATG CCATGGAGAG ACAGCTGCAG AGTGTTGCAG AATTGGAACA GAAATGGCAA 
AGTGAGGTTG ATGATGCCAC GCAGGGGAAA CTGGAGAACA ACATGCCTTT CTTCTATGAT 
TACCATTTTA ATGAGAACAA AATGAAAGAA CTAGAACTTT TGTGTTCAAT GAAGGAAGTC 
TCCTTTGATG GAAATGATCT TGAAAACATG GTCCTATCTC TGAGGGAGAA GTTCCTACAA 
GAAGTGAATT CTCTCATTCA GAAACCCTCG CACCCACTGG CTAAGACGAA GACGTTAGTG 
AAGAGTCTAA TGAACCGAGC TGAGCTGTTG CTGCACGTCA CCATTGCGGC CCAGTCCGGC 
CTCACAAGAA GCATCTCAGG GACCCCCGCA GAAACACCGG CTTGTAAATC AGCCTCTGAA 
ACAAAGGTGA TATCTCATGC TGTGAGGCAG CCTGTTTTTC TTCGCAGCAT GTCGGCTCCT 
TCTGACCTGG AAATGATTGG TAACGATGAT TTGGAATTTA CTAGAGCAAA TCAGAGGCGG 
CGCCACGTGA CCAGCCACCG CAGCAGCTCC TTCACGCTCC TGCAGTCATT GGCCATCGAG 
GACAGCAGGG ACAAGCCCAC CTACAGCGTC CTGCTGGGGC AGCTGTTTGC TTTCATCGGC 
ACCAACCCTG ACCAAGCTGT GTCCAGCAGC AGCTTTCTTT TGGCTGCGCA GACAAGGTGG 
CGACGGGGAA ACACTCGCAA GCAGGCACTG GTGCACATGC GGGAATTGCT GACGGCTGCC 
GTGCGAGTCG GGGGAGTGAC ACATCTTGTG GGTCCGGTGA CTATGGTCCT TCAGGGAGGA 
CCCAGAATTG AAGAACTAAC CTGTGGTGGG ATGGTCGAGC AGGTCCAGGA AGCCTTCGGT 
GAGACCATGA CCTCTGTTGT GTCTTTGTGC GCCCGTTACC CTATTGCTTG TGCGAATAGC 
ATCGGACTCT TATGTACTAT ACCTTACACC AGGAGTGAAG AGAAGTGCCT GGTGCGCAGT 
GGCCTTGTTC AGCTCATGGA TCGACTGTGT AGCTTGAGCA GTCAGACTGA GTCCAGCTCC 
AGTGAGAAAC AAACCAAGAA GCAGAAGGTG GCTACCATGG CCTGGGCAGC CTTCCAGGTG 
CTTGCCAACC GCTGCGTTGA ATGGGAAAAA GAAGAAGGCG GCTCCACAGA GGCTGTGCAC 
TCAGGTCTGG CCCGTCAGGT GTCCAGCCTT CTCACTAACC ACCTCGCCCG AGCGACGGAG 
AGCTGCGGCA ACCAGGCTGC CGGGAACGAT GCCCTCCAGG ACGTCCTGAG TCTTCTCAAT 
GACCTCTCGA GGAGTCACAT AGGTAAAGCC ATCCTGAGCC AGCCAGCTTG TGTGTCCAAA 
CTCCTCTCCC TGCTGCTTGA CCAACGCCCG TCCCCAAAGC TGGTCCTTAT CATTCTTCAG 
CTGTGCCGGG CAGCACTCCC GCTGATGAGC GTAGAAGACT GTGGGAATGT GGAGCTCCCA 
CCCTGGAGCT ACTCTGTCCC CTCCCTAAAC AGTGAGCAAG AGGATCCCAG CGACCCAGCG 
TCCAAGATCG CCTCTTTGCT CTTAGCAAAG CTAGCAGATT ATGTGGTCCC AGGATGTCAG 
ACAGTCCTTT CTCCAACCGC TTCTGAACCT GACACCATGT TGACAAAAAC CAGCCCCAAG 
AGTTCCTTAA AAGGAGATAA AGACCCTGGA GAAGAGAGTG AAGCAGTGGA TGGCAAGCTC 
TCTATCTTTA TCCACAAGCG GGAAGACCAG TCATCCCATG AAGTTCTCCA ACCATTACTA 
AGTAGCTCAG AAGGACGGCC CTTCCGACTT GGTACTGGTG CCAACATGGA GAAAGTCGTG 
AAAATGGACC GAGACATGAC AAAGGGTGGC TGCTGTGAGG TGATTACAGA AGAGGCGGCA 
GCGGCTCTGC GAAAAGCAAC CAAGTGGGCA CAGTCTGGCC TCATCGTCAG CGTCGGGCCA 
CCCGTGGAAT CGCTCAACCC AGAGACTGTG AGTGGACTGT CCACAGGGGA CAAGAAGAAA 
ACCGCCCAAA CTTCCATTTG CCGAGAGAGG AACTCTGAAC TTGCCAGGAC CGACCCTGTC 
CGACCTTTCA TCAGTGGGCA TGTGGCAAAC AGCATGGCTG CAGAGGTGAT CGCCTTGCTT 
CACAGCTTGC TGATGGCGCC TGAGTCAAAT GCTGCTCAGA TATGGACCAC GACAGCAGAG 
AAGGTTCTGT CTCGTGCGCT GATGTACATT CCACAGTTGG GGAAATATGC AGAAAGCATT 
CTGGAAAATG GCAGCAGTAG TGGCAGAAAA CTCGCCAAAC TTCAGAGAAT CGCCCGCCAG 
GCTGTCGCTG CACTGTGTGC ACTTGGAGGC TTCAAAGAGA CAATCAAGAT AGGGTCTGAG 
GTTCAGGTTT TAGGTAGAGG AATATCAGGA AGTGTCGGAG TAGTGGCTTC TATCAATGAA 
CAGGAAGGTA TAGCTACAGT CAAATTCCCA CCCATAGACT GTAGAAAGAC GTCGCAAGCA 
TCAGACACAC TGACTATTCC ATTGTCTAGA CTTTGTGTTC CAAGATCAGA GGCATTGCCT 
CTTCATAAAC TGTCCATTAC TGAGAAGGTA GTACAGGCAG TCCAGTCCAT GTTGCTTCCT 
CAGGAGGGAA GTCTCTCTAT TCATACCTCA CTTCCTGCAA CAGGAGATGG GTCAGCTCCT 
GTGATGGCAG TCGTTCGGCT CCTGGCTGAA ATAAGAACAA GAGCATGCCT GGTCATGGCC 
CAGCTTTTAG AAGACAGCTT ATTTTGTGAA GAATTCATAC AGCAGTGTCC AGCAGCTGTT 
GAGGTTCTCA ACCTAGTAGC CCAGGAATGC AGTGCTGGAG AGCGACTGGC AGTTGTGGAA 
GTGCAGTGTG AACGGCTGAG GATGCTCTAC CGGGACTGTG CTCGGCCCCC GCCACCTCCG 
CTGCAGGCTG ACCGGAGACA GCCCAAAGAG ATTACGTGGA GCCCCTCACG AGTGTTTCCA 
CCTGTCCGAG CCTGCATGTT CTCATCGCAC CTCACTTCCG TCACCTTCCT GGCTGACCCC 
AGCGCTGGGG GCGGTCTGCC CCGGGGCACA TTTATCTATG CCACCTCACC ACTGCCCATT 
CAGGCCCCAT CTTTCTACTG GGAAATTGAA ATTGTTTCCT ATGGAGATAG TGATGATGAC 
ACTGGACCAA TAGTTTCCTT TGGCTTTGCT ACAGAAGCAG AGAAAAGAGA CGGGGCTTGG 
ACTAATCCAG TGGGCACTTG TCTTTTCCAT AACAATGGCC GAGCCGTGCA CTACAACGGC 
TCCAGTTTAT TACAGTGGAA GAGCGTTCGC TTAGACGTGA CTCTCTCTCC TGGTGACGTG 
GCAGGAATTG GCTGGGAGAG AACCGAGGGA ACGCCTCCTC CTCCAGGGCA GCCAGCCAAG 
GGCCGAGTGT ACTTCACATA CTGCGGGCAG CGCCTCTCAC CGTATCTTGA AGATGTTTCG 
GGTGGGATGT GGCCTGTGGT TCACATTCAG AAAAAGAACA CCAAGACGCG AGCTAACTTT 
GGCTCCCGCC CATTTGCCTA TGCTGAAGGG CAGGCCCACC GCAACGCAGC TGACCTGTGC 
ACCGACCTGG CAGAGGAGAT CAGCGCTAAC TTCGAGGCCC TGCCCTTTGC CATGGCATCT 
GACAGTGACA ACGATGCTGG CACCAGTATT GCGTCAGACC CGGGCACTCA CGGGCCACCG 
TGCCGGATTG CTGCCGTGGC CACGGCTCAG CAACAATATG ATAGTGACAC TTCCTGTCAT 
TATAAAGTAG AGCTCAGCTA TGAGAATTTC ATCACCTCCG GCCCAGACCC CCATCCGCCC 
CCCATTGCAG ATGACGAAAG TGATGATGAT GATGACGATG ATATCCCGCA GGAGGACCAC 
TATGCTCTAC TGGTGAAAGC GTGGGAGACC AAGGTTTTTC CTACTATCCG AAGACGCTTC 
CGCAACGAAG CAGAGCGGAA ATCGGGCCTG GACCAGATAA AGGGTGCTTT ACAACTAGGC 
ATGGTGGATA TTGCCCGGCA GACAGTTGAA TTCCTCTACG AGGAGAACGG TGGCATCCCA 
AGAGACCTTT ATCTTCCCAC CATTGAAGAC ATTAAGGATG AAGCAAACAA GTTCACAATT 
GATAAAGTTC GAAAAGGTCT CACGGTAGTA ACCCGCTCTC CAGACAGCAA TAACGTAGCC 
AGCAGTACTG TTGGAACTGC CCTGCCAAAA TTTGCCATCC GAGGGATGCT GAAAACCTTT 
GGGCTTCATG GAGTTGTCTT AGATGTTGAT TCAGTAAATG AATTGGTGCA GGTAGAAACG 
TACCTCCGAA GCGAAGGTGT GCTGGTCCGA TACTGGTATC CTATTGATAT GTTGGAAAGG 
CCCCCAGCAG GCTACCGAAG GACTGCCACC AATGGGCTGG TCACCCTGGA CAATACCAAC 
CTTCAAATTC ACAGGGAGCT GCTGCGCTGC GAGGCCGCAC TGGCCAGGCT GTACTGCCGC 
ATGGCCCTGC TCAACATCTT CGCCCCCAGG CTGCCTCACC TGTTTACCCG CCTCTTCCAC 
ATCCCTGCCA TCCGGGACAT TACCCTGGAG CACCTGCAGC TGCTGTCCAA TCAGCTCCTC 
GCTCCTCCCC TCCCAGATGG CACCATCAGC TCCAGCTCCA TCCTCCTGGC ACAGTCGCTG 
CAGCACTGCA TCCATTCCCA GAACTGCTCG GCCACGGACC TCTTCTACCA GGGCAGCTCC 
CAGACAGTGA GAGAGTGGCT CAACGTGGCC ATCACTCGGA CCCTGCACCA GGGTGAGGAG 
AGCCTGTTGG AGCTGACGAA ACAGATCTGC TCTTTCCTGC AGACGGCACC AGAGCAGTTC 
CCCTCCGAAG AGTTTCCAAT TTCCGAGTCC AAGGTCAACA TGGACGTCAA TTTCCCCGGG 
GCGGCTTTCG TCGTGGTATC TTGCAAAGAA AGTCAGTCTG GATTCCGCAA AGACTCCTCT 
CTGTACAAGG CGCCGTGGGC GCGCGTGCTC GTGTACGGAC TGGGCCACAA AGTGAAGCGA 
AATGGCCAGC TAAACCTCAT CGAGGCCATC TGTTACCCGC GGGATGCGTC TCCGGCCAAC 
ACGGGGCTCA CGCCGCCTCC CACCACCAAC CAGTACCCTT CTGTGATCCT CTCCACAGAC 
AAAGTCCACA TCAAACTGGG GGTGTCTCCC CCTCCTGGAG CCGTGCTGGT GTTACACTCT 
CTGCCCCTTG AGTTCCCACT GGCGATGGCC TTCGCAGAGC AGCTGCTGTC CTGGAAGTCG 
GAGGACGGTG AAGGGAGATC TGAAGACGAG CCTGACACCA TCCCGACGTC CGTCCTCCTG 
CAAGTGGTGG AACTGCTCGG AAACTTCCTG TGGACCACGG ACATGGCGGC CTGTGTGAAA 
GAGCTGGTTT TCCACCTCCT GGCAGAGCTC GTGCGCACAG TGCACAGCCT GGAGCAGAGG 
AAGCACCCTG CCGGCCTGTC CTCGTCCATC GCCCTCCAGC TGAACCCCTG CCTGGCCATG 
CTGATGGCCC TGCAGTCAGA GCTCCACAAG CTGTACGACG AGGAGACGCA GAGCTGGGTC 
TCGGGCAGCA CGTGCGGAGG CTCGGGGACC GTGGCGGCCA CCGACCAGGG CAGGTTCTCT 
ACGTATTTCC ACGCGCTCAT GGAAGGGTGC CTGGCGGTGG CCGAGGTGAC CCTGCCTACC 
AACATGAGCG TCACTGCCAG CGGGGTGACG TCGGCGACCG CCCCAAACCT CAGTGACTCG 
TCGTCCTCCT CGTCATCCTC CCCAGGACAG ACGCCGCAGA GCCCCAGCCT CCTGTCCAAG 
AGGAAGAAAG TCAAGATGAA GCGGGAAAAG GCCTCCTCCT CTGGGAAGCG CCAGTCCTCG 
CACTCAGTGG ACTCGGACCC CGCCGTGCTG AGCATCGGGG GTGGCAAGCC CGAAGACATG 
CTGTGGTTCC ATCGCGCCCT GACCTTGCTC ATCATCCTCC GGCACCTCAC CAGGAAGGAC 
CCGCAGGGGC TGGGTGTGAC GAGCGACGCC ATCGCCGACG CCTGCCAGGC CCTGGTGGGT 
CCCACCGCCC ACAGCCGCTT GCTGGTGATC TCTGGGATCC CCACCCACCT GGACGAGGGC 
ATCGTCAGAG GCGCCATCCG CAAGGCCTGC AACGCCCACG GCGGGGTCTT CAAAGACGAG 
ATCTACATCC CCCTGCAGGA CGAAGACCCC AAGAAGCCAA AAGACAAGGC CGAGGGCGGT 
GATGGGAAAG CCGAGCCAGA GAAGACCCTT GGCTTCCCCA GCACAGACAG CTTGGAGGTC 
AGCACGTCCC GCAGCCCGAC CCCCGCCATG AGCATCAGCG CGTCTGCGTC CACCAGCCAA 
GCCTCCATCT GCAGCTCCCA GGGCATCTTG CAGACAGTCA GCGATGTCTC TGTGGAACCG 
CTGCCCTCGG GCCTCGAGCT GCCCATCCCT GCGGGCTTAT TGGAGCCCCA CGTAGTGTCC 
AGCCAGGAGA GCCTGGACGT TTCCCTGTGC AGCACCAGCA GCCTGGGCAG CCTCGGGAGC 
CTGGGGGAGC CACTGGACAA CGCAGAAACG GCCTCAGTGT CAGACATGGG CTCCATGTAC 
ACAGTCACGT CCCTGGACAA CCAGCCCCTC GCAGCACGCC CCATCAAAGG CTTCGCGGTC 
GTGGAGATTA GATCCCGAGC CAAAATCGAG AAAATCCGAG CGAGTTTATT TAACAATAAT 
GACTTGATTG GTTTGTCAAG TTTGGATGGT GAAGATGAAT TGATGGAAAT GTCAACGGAA 
GAGATCCTCA CTGTGTCTGT AGTAAATCAG AGTTTGTTTG ACACGCAGGG GAGCCCAGGG 
TTAGAAGATT ATTTCAATGA TAAGTCAATT AAAGGTGAGA AGCTGGTGCC CGGGGCCAGA 
GAGGTGCTGA CGGAGATATT TAAGAGCTGC GCCCACTCAG AGCAGATGCT GAGTCTGACA 
CCAGCAAAGC CCATCAAAGT GTCTGACATT TATCTCAGCA AAGAGCAGAT CAACTCCCAG 
ACGCCAGGCA ACCTCCTCCA CCTCTTCTTC ACCAATGTTC GGCCCCCCAA AAAGGTGCTG 
GAGGACCAGC TCACCCAGAT CCTGAGGAAG TACGGCGTGC CAAAACCCAA GTTCGACAAG 
AGCAAGTACA GCAAGGCGGG GAAGGAACAG CACCCCGGGA AGGTGGTGAG CACCAAGCGG 
CCCATCACCA AGCCACCTGC CAAGGACAAG GCCGTGCTCA ACAGCGTCAG CAGGACTGCC 
TTGAGCGAGA AGAAGCCCAC TGTGAAGCCG AAGTCACCGG AAAAGAGCAA GCCGGACGAA 
AAGGACCCGG AAAAGTCACC CACCAAAAAA CAAGAAGTCC CTGAGGAAAA GTACCTGACA 
TTGGAAGGAT TTCACAAGTT TGTTATTGAC CGAGCCAAGC AAGACATCCG TAGTGTCTGG 
AGGGCGATTT TATCCTGTGG TTACGATCTT CATTTTGAGA GGTGTGCCTG CATCGACGTC 
CGGCACGCAC ACAAGGCCTC CAGGAAGTGG ACCCTGGAGA TGGACGTGGC GCTCGTGCAG 
TACATCAACC GGCTGTGCCG CCACCTGGCC ATCACGCCCG CGCGGCTGCA TCCCCACGAG 
GTGTATCTGG AGACCACAGA CGCCGCCGAC CCCAGAGTGG CCTGTCTCTT GAATGTGCCC 
ATTGAGAGCC TGCGCCTGCG CTTCGCCCTG CTGCAGTCCC TGAACACCAC GCTCGAGACC 
TTCTTCCTGC CACTCGTGGA GCTGCGCCAG ACGCCCATGT ACACGCACAG CATCGCGGCC 
CTGCTGAAGG AGGCCAAAGG GCTGATCTTT TATGACACGA AGGTGACCGT CATGAATCGG 
GTGCTGAACG CCACAGTGCA GAGGACGGCT GACCACGCGG CACCGGAGAT TACTCTGGAC 
CCACTGGAGA TCGTGGGAGG GGAGATCAGA GCCTCTGAAA ACTCCTACTT CTGCCAGGCG 
GCCAGGCAGC TGGCCTCGGT GCCATCCTCC CAGCTTTGCG TCAGACTGGC GAGTGGCGGT 
GACCCCACCT ATGCCTTCAA CATCCGCTTC ACTGGGGAGG AGGTCCACGG CACCAGCGGC 
TCCTTCCGCC ACTTCCTGTG GCAGGTGTGT AAAGAGCTGC AGAGCTCCGC GCTCTCCCTT 
CTCCTGCTGT GCCCCAGCTC AGCCGTCAAC AAGAACAAGG GCCAGTACAT CCTGAGCCCA 
AGCCCCATCA GCTACGGGGA GGAGCAGCTG CTGCACTTCC TGGGCCAGTT GCTGGGAATT 
GCGATTCGAG CAGATGTCCC TCTTCCCCTG GACCTCCTAC CCTCCTTCTG GAAGACGCTG 
GTGGGCGAGC CCCTGGACCC TGACCAAGAC CTGCAGGAAG CAGACATACT CACCTACAAT 
TATGTCAAGA AATTCGAGAG TATCAACGAC GAGAGCGAGC TGGAGGCCCT GTGCGCCGAG 
ATTGCCTCCC AGCACCTGGC CATCGAGAGC CCCGACGGGC CCAGTAAGCC CTGCTGCCGG 
TTCACCTACC TGACCATGGC GGGCGAGGAG GTGGAGCTGT GCAGCCGCGG CCGGCACATC 
CCTGTGGCGT GGGAGAACAA GGACATCTAC GCAGCAGCCA TCCGGAGCCT GCGGCTGCGG 
GAGCTGCAGA ACGTCGAGTG TGTGACAGCC GTGCGCGCGG GCCTCGGCGC CATCATCCCC 
CTGCAGCTGC TCACCACGCT CAGCCCGCTG GAGATGGAGC TGCGGACGTG CGGCCTCCCT 
TACATCAACC TCGAGTTCCT GAAGGCCCAC ACCATGTACC AGGTGGGCCT GATGGAGACG 
GACCAGCACA TCGAGTTCTT CTGGGGGGCA CTGGAGATGT TTGCCCAGGA GGAGCTGTGC 
AAGTTCATCA AGTTTGCCTG CAACCAAGAG CGCATCCCGT TCACCTGCCC CTGCAAAGAC 
GGGGGCCCCG ACACAGCCCA CGTGCCCCCA TACCCCATGA AGATCGCCCC CCCGGATGGC 
ACAGCAGGTT CCCCAGACTC TCGCTACATC CGAGTGGAGA CCTGCATGTT CATGATCAAG 
CTTCCCCAGT ACTCCTCTCT GGAAATCATG CTGGAGAAAC TTCGTTGTGC CATTCACTAT 
CGCGAAGACC CCCTGAGTGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028336093.1
CDS	156..13439
Translation	MGSSAAAAAAAAAAADSAQWLSVKEETIFLHDGLIRVTDLAELPSEILGAPEAADTDLEILTFETKNPTELAERLRSVCGNQSNAYARLLEYRLNALRGLWNAQRQLALEEQHERESSGDEETLALLKRQGLLQQPEQAPFTSRMGLLLVFPLIQSQSRTDPSLCNITAEVLLNCLRDCQPLSLTKEPADCLNGIETLLCSWLEETSDTGRHIPHKQKENAAAALVALACARGSLKTFVHTVHLLQKQTDLGSLPVADVLYRLLLLEGGPGSPSCLLGGKHIVSWGYEDMLPAPDSNTGSSSENKDADLGRCVTADGLYLYTTNSVGRGVSKLGSGLHGTLRGFVYCRNEELEPGWIAFGSGSLLHRPVSFDNKPHSLFQVIDQNTLQVCQMVPMPANHLPVGSTMSTVHLSSDGTYFYWIWSPASLNEKTPKGHSVFMDIFELVVENGVFVANPLQERTILMRKEGESAKSINEMLLSRLSRYRASPSATLAALTGSTISNTLKEDQAANTSCGLPLKMLRKTPIYTCGTYLVMLVPPPGGSGSSATRSLFGGTSGLSSLKILASSLVYNISDGQFASRADLIDAAGSSLGRGALVPGLGACYDTVNNMLWTCSNDYIDQWCNPGNQAFHYVCQRLGVSHIITEPKEEAITTNEVINQLLHHVGAMCIHQLNLLATNPNLPITSVLGKQHPIEAHHLSSICDIMEKAMVNGDTCIIRCILVVFQVVFKFFFSPQTERNRDIIRRSGLLLWQLLMAPKDQICPEIQKEVCLAISSGLNILYPGETEINNLLKLVLTEGERNSGLSQLRDVILTNLAEQLQNNRFGSEEDDHYRLNDELLHYILKIVVRESCILITKCQTVSKDDFQKLLSTVPAASSCLRYLMAVQNHLLSNTVLIKPDENDDSDNSLQGETLKVQELKVSILALATQILTGCDEVLEMLQQVTTALINSDIADREQRLKGLEQVTKATMLGHLLPVLLTSLMHPNLQTLTMADALMPQLVQLVLYTSQTALLLKTQCPVFAEAGCSPCGASDQKGRLFPDERMLEEKEEPGFLTGLKIPAPWAAGKTVETVHPVRDNYKFKETVHIPGARCLYLRFDSRCSSQYDYDKSNSIFSVLKLVIYAGPNTNSRKVAEYGGNTLGYGSRSVLGTGWPKDLVKVEGDTVTFSFEMRSGREHNTPDKAMWGFACTVRAQESSEDVSGGLPFLVDLALGLSVLACSMLRILYNGPEITKEEEACQELLRSKLLQRCQWQVEANGVISPALTPSPSPLPLTIEEDREFTYPSDVLVPPVGNYFDLPRIRLPPGIMIKLREISGRARPQFRPSIKEVIQPDVMEEMVVSCVIKHLNLVDALQSLINFQYQEEHAEEYDLLCKIMGETFKKLNAMERQLQSVAELEQKWQSEVDDATQGKLENNMPFFYDYHFNENKMKELELLCSMKEVSFDGNDLENMVLSLREKFLQEVNSLIQKPSHPLAKTKTLVKSLMNRAELLLHVTIAAQSGLTRSISGTPAETPACKSASETKVISHAVRQPVFLRSMSAPSDLEMIGNDDLEFTRANQRRRHVTSHRSSSFTLLQSLAIEDSRDKPTYSVLLGQLFAFIGTNPDQAVSSSSFLLAAQTRWRRGNTRKQALVHMRELLTAAVRVGGVTHLVGPVTMVLQGGPRIEELTCGGMVEQVQEAFGETMTSVVSLCARYPIACANSIGLLCTIPYTRSEEKCLVRSGLVQLMDRLCSLSSQTESSSSEKQTKKQKVATMAWAAFQVLANRCVEWEKEEGGSTEAVHSGLARQVSSLLTNHLARATESCGNQAAGNDALQDVLSLLNDLSRSHIGKAILSQPACVSKLLSLLLDQRPSPKLVLIILQLCRAALPLMSVEDCGNVELPPWSYSVPSLNSEQEDPSDPASKIASLLLAKLADYVVPGCQTVLSPTASEPDTMLTKTSPKSSLKGDKDPGEESEAVDGKLSIFIHKREDQSSHEVLQPLLSSSEGRPFRLGTGANMEKVVKMDRDMTKGGCCEVITEEAAAALRKATKWAQSGLIVSVGPPVESLNPETVSGLSTGDKKKTAQTSICRERNSELARTDPVRPFISGHVANSMAAEVIALLHSLLMAPESNAAQIWTTTAEKVLSRALMYIPQLGKYAESILENGSSSGRKLAKLQRIARQAVAALCALGGFKETIKIGSEVQVLGRGISGSVGVVASINEQEGIATVKFPPIDCRKTSQASDTLTIPLSRLCVPRSEALPLHKLSITEKVVQAVQSMLLPQEGSLSIHTSLPATGDGSAPVMAVVRLLAEIRTRACLVMAQLLEDSLFCEEFIQQCPAAVEVLNLVAQECSAGERLAVVEVQCERLRMLYRDCARPPPPPLQADRRQPKEITWSPSRVFPPVRACMFSSHLTSVTFLADPSAGGGLPRGTFIYATSPLPIQAPSFYWEIEIVSYGDSDDDTGPIVSFGFATEAEKRDGAWTNPVGTCLFHNNGRAVHYNGSSLLQWKSVRLDVTLSPGDVAGIGWERTEGTPPPPGQPAKGRVYFTYCGQRLSPYLEDVSGGMWPVVHIQKKNTKTRANFGSRPFAYAEGQAHRNAADLCTDLAEEISANFEALPFAMASDSDNDAGTSIASDPGTHGPPCRIAAVATAQQQYDSDTSCHYKVELSYENFITSGPDPHPPPIADDESDDDDDDDIPQEDHYALLVKAWETKVFPTIRRRFRNEAERKSGLDQIKGALQLGMVDIARQTVEFLYEENGGIPRDLYLPTIEDIKDEANKFTIDKVRKGLTVVTRSPDSNNVASSTVGTALPKFAIRGMLKTFGLHGVVLDVDSVNELVQVETYLRSEGVLVRYWYPIDMLERPPAGYRRTATNGLVTLDNTNLQIHRELLRCEAALARLYCRMALLNIFAPRLPHLFTRLFHIPAIRDITLEHLQLLSNQLLAPPLPDGTISSSSILLAQSLQHCIHSQNCSATDLFYQGSSQTVREWLNVAITRTLHQGEESLLELTKQICSFLQTAPEQFPSEEFPISESKVNMDVNFPGAAFVVVSCKESQSGFRKDSSLYKAPWARVLVYGLGHKVKRNGQLNLIEAICYPRDASPANTGLTPPPTTNQYPSVILSTDKVHIKLGVSPPPGAVLVLHSLPLEFPLAMAFAEQLLSWKSEDGEGRSEDEPDTIPTSVLLQVVELLGNFLWTTDMAACVKELVFHLLAELVRTVHSLEQRKHPAGLSSSIALQLNPCLAMLMALQSELHKLYDEETQSWVSGSTCGGSGTVAATDQGRFSTYFHALMEGCLAVAEVTLPTNMSVTASGVTSATAPNLSDSSSSSSSSPGQTPQSPSLLSKRKKVKMKREKASSSGKRQSSHSVDSDPAVLSIGGGKPEDMLWFHRALTLLIILRHLTRKDPQGLGVTSDAIADACQALVGPTAHSRLLVISGIPTHLDEGIVRGAIRKACNAHGGVFKDEIYIPLQDEDPKKPKDKAEGGDGKAEPEKTLGFPSTDSLEVSTSRSPTPAMSISASASTSQASICSSQGILQTVSDVSVEPLPSGLELPIPAGLLEPHVVSSQESLDVSLCSTSSLGSLGSLGEPLDNAETASVSDMGSMYTVTSLDNQPLAARPIKGFAVVEIRSRAKIEKIRASLFNNNDLIGLSSLDGEDELMEMSTEEILTVSVVNQSLFDTQGSPGLEDYFNDKSIKGEKLVPGAREVLTEIFKSCAHSEQMLSLTPAKPIKVSDIYLSKEQINSQTPGNLLHLFFTNVRPPKKVLEDQLTQILRKYGVPKPKFDKSKYSKAGKEQHPGKVVSTKRPITKPPAKDKAVLNSVSRTALSEKKPTVKPKSPEKSKPDEKDPEKSPTKKQEVPEEKYLTLEGFHKFVIDRAKQDIRSVWRAILSCGYDLHFERCACIDVRHAHKASRKWTLEMDVALVQYINRLCRHLAITPARLHPHEVYLETTDAADPRVACLLNVPIESLRLRFALLQSLNTTLETFFLPLVELRQTPMYTHSIAALLKEAKGLIFYDTKVTVMNRVLNATVQRTADHAAPEITLDPLEIVGGEIRASENSYFCQAARQLASVPSSQLCVRLASGGDPTYAFNIRFTGEEVHGTSGSFRHFLWQVCKELQSSALSLLLLCPSSAVNKNKGQYILSPSPISYGEEQLLHFLGQLLGIAIRADVPLPLDLLPSFWKTLVGEPLDPDQDLQEADILTYNYVKKFESINDESELEALCAEIASQHLAIESPDGPSKPCCRFTYLTMAGEEVELCSRGRHIPVAWENKDIYAAAIRSLRLRELQNVECVTAVRAGLGAIIPLQLLTTLSPLEMELRTCGLPYINLEFLKAHTMYQVGLMETDQHIEFFWGALEMFAQEELCKFIKFACNQERIPFTCPCKDGGPDTAHVPPYPMKIAPPDGTAGSPDSRYIRVETCMFMIKLPQYSSLEIMLEKLRCAIHYREDPLSG

Target ORF information:

RefSeq Version	XM_028480292.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon HECT domain E3 ubiquitin protein ligase 4 (HECTD4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028480292.1

ORF Insert Sequence:

ATGGGCTCGT CGGCAGCCGC GGCGGCTGCG GCGGCGGCGG CCGCGGACTC GGCGCAGTGG 
CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG 
GCCGAGCTGC CCAGCGAGAT TCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTGGAGATT 
TTAACATTTG AGACCAAGAA TCCGACGGAA TTAGCAGAAC GTTTGCGCTC TGTTTGTGGG 
AATCAGAGCA ATGCGTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG 
TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAAAG TTCGGGTGAC 
GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC 
TTCACGTCGA GGATGGGGCT TCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA 
GACCCCTCTC TCTGTAACAT AACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG 
CCTTTGAGCT TGACCAAGGA GCCTGCTGAC TGTCTGAATG GAATCGAAAC TTTGCTGTGC 
TCTTGGCTAG AGGAGACTTC TGACACAGGC CGACACATCC CACATAAGCA AAAAGAAAAT 
GCTGCTGCTG CCCTGGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT 
ACAGTCCATC TATTACAGAA ACAGACAGAT CTAGGGTCCC TGCCTGTAGC CGATGTGCTG 
TATAGGCTGT TGCTTTTGGA AGGGGGACCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA 
CACATAGTAT CATGGGGGTA TGAAGACATG TTGCCTGCAC CAGATAGCAA CACTGGCTCC 
AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTGTCA CGGCAGATGG TCTTTACCTG 
TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT 
CTCAGAGGTT TTGTGTACTG CCGGAATGAG GAGCTGGAAC CAGGATGGAT TGCTTTTGGC 
AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC TCTTTTCCAG 
GTCATTGACC AGAATACCCT TCAGGTGTGC CAGATGGTGC CAATGCCAGC CAATCACCTC 
CCTGTGGGCA GCACTATGAG CACCGTGCAC CTTTCTTCAG ATGGTACTTA CTTCTATTGG 
ATCTGGTCTC CTGCCAGTCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC 
ATTTTTGAAC TTGTGGTTGA AAATGGTGTA TTTGTAGCCA ACCCGCTTCA GGAACGCACA 
ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT TCTGAGCAGA 
CTTTCACGCT ACCGAGCCTC GCCATCCGCA ACGCTGGCTG CCCTTACCGG CTCCACCATC 
TCCAACACCC TGAAAGAGGA CCAGGCAGCA AATACTAGCT GTGGGCTTCC TCTAAAAATG 
CTTCGGAAGA CGCCCATTTA CACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA 
GGGGGATCAG GCAGCTCTGC TACCCGGTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT 
TTAAAAATCC TAGCTTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CGCAAGTCGT 
GCTGATCTCA TAGATGCTGC TGGAAGCTCA TTGGGGCGTG GTGCTCTTGT ACCAGGATTA 
GGTGCCTGTT ACGATACAGT GAACAACATG CTATGGACAT GCTCGAATGA CTATATCGAT 
CAATGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GTCAGAGACT TGGAGTCAGC 
CATATCATCA CTGAGCCTAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA 
TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT 
CTTCCAATCA CAAGTGTCTT GGGCAAGCAG CATCCAATTG AAGCACACCA TCTTAGCAGT 
ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATCAT ACGCTGCATT 
CTCGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA 
GACATCATTC GACGGTCGGG GTTGCTTCTT TGGCAGTTGT TGATGGCACC AAAAGATCAA 
ATTTGTCCTG AAATTCAGAA GGAAGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG 
TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA 
AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTAACCA ATTTAGCTGA ACAGCTTCAA 
AACAACCGAT TTGGCAGTGA GGAGGATGAT CATTACAGAC TAAATGACGA ACTTTTACAC 
TACATTTTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC 
TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CTGCATCCTC CTGCCTGCGC 
TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTG TTTTGATTAA ACCTGATGAG 
AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGTACAGGA GCTAAAGGTC 
AGTATTTTGG CTCTTGCCAC CCAAATCCTG ACTGGATGTG ATGAAGTGTT GGAAATGCTA 
CAGCAGGTCA CAACTGCCCT CATAAATAGT GACATAGCTG ACCGGGAGCA GAGGTTAAAA 
GGCTTGGAAC AAGTTACTAA GGCTACTATG CTCGGTCACC TTCTTCCTGT GTTACTGACC 
TCCCTGATGC ATCCAAATTT ACAGACTCTG ACCATGGCCG ATGCCCTGAT GCCTCAGCTA 
GTGCAGCTGG TACTTTATAC CAGTCAGACG GCTTTGCTGC TCAAAACCCA GTGTCCGGTT 
TTTGCTGAGG CGGGCTGTTC CCCATGTGGT GCATCAGACC AGAAGGGCAG GCTGTTCCCT 
GATGAGAGAA TGCTGGAAGA GAAGGAAGAG CCAGGGTTTC TCACTGGTTT AAAGATTCCT 
GCTCCATGGG CTGCTGGGAA GACTGTGGAA ACAGTCCATC CTGTCAGAGA CAACTATAAA 
TTTAAAGAAA CTGTCCATAT CCCGGGAGCT CGCTGCCTAT ACCTTAGATT TGATAGCAGA 
TGCTCTTCAC AATATGACTA TGACAAATCT AACAGTATAT TTTCTGTTTT GAAGTTGGTG 
ATATACGCGG GGCCTAACAC AAACAGTAGG AAGGTTGCTG AGTATGGAGG CAACACTCTG 
GGATATGGCA GCCGTAGTGT CTTAGGAACT GGTTGGCCGA AAGACTTGGT GAAGGTGGAA 
GGAGATACAG TCACCTTCTC CTTTGAAATG AGAAGTGGCC GTGAACACAA CACTCCCGAT 
AAAGCTATGT GGGGCTTTGC TTGCACAGTT CGCGCTCAGG AGTCTTCAGA GGATGTGTCG 
GGAGGCTTAC CTTTTCTGGT AGACCTGGCT TTAGGTCTGT CTGTGTTAGC TTGTTCTATG 
TTAAGAATCC TATACAACGG ACCAGAAATT ACCAAAGAAG AAGAAGCCTG TCAGGAGCTG 
CTGCGGTCCA AACTTTTACA AAGGTGCCAG TGGCAGGTGG AGGCCAATGG TGTGATCTCC 
CCTGCCCTTA CTCCAAGTCC GTCTCCACTG CCTCTGACCA TCGAGGAAGA CAGAGAATTC 
ACTTACCCCT CTGACGTCCT CGTGCCTCCT GTTGGAAACT ACTTTGACCT GCCCCGGATC 
AGGCTGCCTC CAGGAATCAT GATAAAGCTC AGGGAAATTT CTGGGCGTGC CAGACCTCAA 
TTTAGACCAA GTATAAAAGA AGTAATCCAG CCAGACGTGA TGGAGGAAAT GGTGGTATCG 
TGTGTCATTA AGCACTTGAA CTTGGTTGAT GCACTGCAGT CCCTAATAAA TTTCCAGTAT 
CAAGAAGAAC ATGCTGAGGA ATATGATTTA TTGTGTAAAA TCATGGGAGA GACATTTAAG 
AAACTCAATG CCATGGAGAG ACAGCTGCAG AGTGTTGCAG AATTGGAACA GAAATGGCAA 
AGTGAGGTTG ATGATGCCAC GCAGGGGAAA CTGGAGAACA ACATGCCTTT CTTCTATGAT 
TACCATTTTA ATGAGAACAA AATGAAAGAA CTAGAACTTT TGTGTTCAAT GAAGGAAGTC 
TCCTTTGATG GAAATGATCT TGAAAACATG GTCCTATCTC TGAGGGAGAA GTTCCTACAA 
GAAGTGAATT CTCTCATTCA GAAACCCTCG CACCCACTGG CTAAGACGAA GACGTTAGTG 
AAGAGTCTAA TGAACCGAGC TGAGCTGTTG CTGCACGTCA CCATTGCGGC CCAGTCCGGC 
CTCACAAGAA GCATCTCAGG GACCCCCGCA GAAACACCGG CTTGTAAATC AGCCTCTGAA 
ACAAAGGTGA TATCTCATGC TGTGAGGCAG CCTGTTTTTC TTCGCAGCAT GTCGGCTCCT 
TCTGACCTGG AAATGATTGG TAACGATGAT TTGGAATTTA CTAGAGCAAA TCAGAGGCGG 
CGCCACGTGA CCAGCCACCG CAGCAGCTCC TTCACGCTCC TGCAGTCATT GGCCATCGAG 
GACAGCAGGG ACAAGCCCAC CTACAGCGTC CTGCTGGGGC AGCTGTTTGC TTTCATCGGC 
ACCAACCCTG ACCAAGCTGT GTCCAGCAGC AGCTTTCTTT TGGCTGCGCA GACAAGGTGG 
CGACGGGGAA ACACTCGCAA GCAGGCACTG GTGCACATGC GGGAATTGCT GACGGCTGCC 
GTGCGAGTCG GGGGAGTGAC ACATCTTGTG GGTCCGGTGA CTATGGTCCT TCAGGGAGGA 
CCCAGAATTG AAGAACTAAC CTGTGGTGGG ATGGTCGAGC AGGTCCAGGA AGCCTTCGGT 
GAGACCATGA CCTCTGTTGT GTCTTTGTGC GCCCGTTACC CTATTGCTTG TGCGAATAGC 
ATCGGACTCT TATGTACTAT ACCTTACACC AGGAGTGAAG AGAAGTGCCT GGTGCGCAGT 
GGCCTTGTTC AGCTCATGGA TCGACTGTGT AGCTTGAGCA GTCAGACTGA GTCCAGCTCC 
AGTGAGAAAC AAACCAAGAA GCAGAAGGTG GCTACCATGG CCTGGGCAGC CTTCCAGGTG 
CTTGCCAACC GCTGCGTTGA ATGGGAAAAA GAAGAAGGCG GCTCCACAGA GGCTGTGCAC 
TCAGGTCTGG CCCGTCAGGT GTCCAGCCTT CTCACTAACC ACCTCGCCCG AGCGACGGAG 
AGCTGCGGCA ACCAGGCTGC CGGGAACGAT GCCCTCCAGG ACGTCCTGAG TCTTCTCAAT 
GACCTCTCGA GGAGTCACAT AGGTAAAGCC ATCCTGAGCC AGCCAGCTTG TGTGTCCAAA 
CTCCTCTCCC TGCTGCTTGA CCAACGCCCG TCCCCAAAGC TGGTCCTTAT CATTCTTCAG 
CTGTGCCGGG CAGCACTCCC GCTGATGAGC GTAGAAGACT GTGGGAATGT GGAGCTCCCA 
CCCTGGAGCT ACTCTGTCCC CTCCCTAAAC AGTGAGCAAG AGGATCCCAG CGACCCAGCG 
TCCAAGATCG CCTCTTTGCT CTTAGCAAAG CTAGCAGATT ATGTGGTCCC AGGATGTCAG 
ACAGTCCTTT CTCCAACCGC TTCTGAACCT GACACCATGT TGACAAAAAC CAGCCCCAAG 
AGTTCCTTAA AAGGAGATAA AGACCCTGGA GAAGAGAGTG AAGCAGTGGA TGGCAAGCTC 
TCTATCTTTA TCCACAAGCG GGAAGACCAG TCATCCCATG AAGTTCTCCA ACCATTACTA 
AGTAGCTCAG AAGGACGGCC CTTCCGACTT GGTACTGGTG CCAACATGGA GAAAGTCGTG 
AAAATGGACC GAGACATGAC AAAGGGTGGC TGCTGTGAGG TGATTACAGA AGAGGCGGCA 
GCGGCTCTGC GAAAAGCAAC CAAGTGGGCA CAGTCTGGCC TCATCGTCAG CGTCGGGCCA 
CCCGTGGAAT CGCTCAACCC AGAGACTGTG AGTGGACTGT CCACAGGGGA CAAGAAGAAA 
ACCGCCCAAA CTTCCATTTG CCGAGAGAGG AACTCTGAAC TTGCCAGGAC CGACCCTGTC 
CGACCTTTCA TCAGTGGGCA TGTGGCAAAC AGCATGGCTG CAGAGGTGAT CGCCTTGCTT 
CACAGCTTGC TGATGGCGCC TGAGTCAAAT GCTGCTCAGA TATGGACCAC GACAGCAGAG 
AAGGTTCTGT CTCGTGCGCT GATGTACATT CCACAGTTGG GGAAATATGC AGAAAGCATT 
CTGGAAAATG GCAGCAGTAG TGGCAGAAAA CTCGCCAAAC TTCAGAGAAT CGCCCGCCAG 
GCTGTCGCTG CACTGTGTGC ACTTGGAGGC TTCAAAGAGA CAATCAAGAT AGGGTCTGAG 
GTTCAGGTTT TAGGTAGAGG AATATCAGGA AGTGTCGGAG TAGTGGCTTC TATCAATGAA 
CAGGAAGGTA TAGCTACAGT CAAATTCCCA CCCATAGACT GTAGAAAGAC GTCGCAAGCA 
TCAGACACAC TGACTATTCC ATTGTCTAGA CTTTGTGTTC CAAGATCAGA GGCATTGCCT 
CTTCATAAAC TGTCCATTAC TGAGAAGGTA GTACAGGCAG TCCAGTCCAT GTTGCTTCCT 
CAGGAGGGAA GTCTCTCTAT TCATACCTCA CTTCCTGCAA CAGGAGATGG GTCAGCTCCT 
GTGATGGCAG TCGTTCGGCT CCTGGCTGAA ATAAGAACAA GAGCATGCCT GGTCATGGCC 
CAGCTTTTAG AAGACAGCTT ATTTTGTGAA GAATTCATAC AGCAGTGTCC AGCAGCTGTT 
GAGGTTCTCA ACCTAGTAGC CCAGGAATGC AGTGCTGGAG AGCGACTGGC AGTTGTGGAA 
GTGCAGTGTG AACGGCTGAG GATGCTCTAC CGGGACTGTG CTCGGCCCCC GCCACCTCCG 
CTGCAGGCTG ACCGGAGACA GCCCAAAGAG ATTACGTGGA GCCCCTCACG AGTGTTTCCA 
CCTGTCCGAG CCTGCATGTT CTCATCGCAC CTCACTTCCG TCACCTTCCT GGCTGACCCC 
AGCGCTGGGG GCGGTCTGCC CCGGGGCACA TTTATCTATG CCACCTCACC ACTGCCCATT 
CAGGCCCCAT CTTTCTACTG GGAAATTGAA ATTGTTTCCT ATGGAGATAG TGATGATGAC 
ACTGGACCAA TAGTTTCCTT TGGCTTTGCT ACAGAAGCAG AGAAAAGAGA CGGGGCTTGG 
ACTAATCCAG TGGGCACTTG TCTTTTCCAT AACAATGGCC GAGCCGTGCA CTACAACGGC 
TCCAGTTTAT TACAGTGGAA GAGCGTTCGC TTAGACGTGA CTCTCTCTCC TGGTGACGTG 
GCAGGAATTG GCTGGGAGAG AACCGAGGGA ACGCCTCCTC CTCCAGGGCA GCCAGCCAAG 
GGCCGAGTGT ACTTCACATA CTGCGGGCAG CGCCTCTCAC CGTATCTTGA AGATGTTTCG 
GGTGGGATGT GGCCTGTGGT TCACATTCAG AAAAAGAACA CCAAGACGCG AGCTAACTTT 
GGCTCCCGCC CATTTGCCTA TGCTGAAGGG CAGGCCCACC GCAACGCAGC TGACCTGTGC 
ACCGACCTGG CAGAGGAGAT CAGCGCTAAC TTCGAGGCCC TGCCCTTTGC CATGGCATCT 
GACAGTGACA ACGATGCTGG CACCAGTATT GCGTCAGACC CGGGCACTCA CGGGCCACCG 
TGCCGGATTG CTGCCGTGGC CACGGCTCAG CAACAATATG ATAGTGACAC TTCCTGTCAT 
TATAAAGTAG AGCTCAGCTA TGAGAATTTC ATCACCTCCG GCCCAGACCC CCATCCGCCC 
CCCATTGCAG ATGACGAAAG TGATGATGAT GATGACGATG ATATCCCGCA GGAGGACCAC 
TATGCTCTAC TGGTGAAAGC GTGGGAGACC AAGGTTTTTC CTACTATCCG AAGACGCTTC 
CGCAACGAAG CAGAGCGGAA ATCGGGCCTG GACCAGATAA AGGGTGCTTT ACAACTAGGC 
ATGGTGGATA TTGCCCGGCA GACAGTTGAA TTCCTCTACG AGGAGAACGG TGGCATCCCA 
AGAGACCTTT ATCTTCCCAC CATTGAAGAC ATTAAGGATG AAGCAAACAA GTTCACAATT 
GATAAAGTTC GAAAAGGTCT CACGGTAGTA ACCCGCTCTC CAGACAGCAA TAACGTAGCC 
AGCAGTACTG TTGGAACTGC CCTGCCAAAA TTTGCCATCC GAGGGATGCT GAAAACCTTT 
GGGCTTCATG GAGTTGTCTT AGATGTTGAT TCAGTAAATG AATTGGTGCA GGTAGAAACG 
TACCTCCGAA GCGAAGGTGT GCTGGTCCGA TACTGGTATC CTATTGATAT GTTGGAAAGG 
CCCCCAGCAG GCTACCGAAG GACTGCCACC AATGGGCTGG TCACCCTGGA CAATACCAAC 
CTTCAAATTC ACAGGGAGCT GCTGCGCTGC GAGGCCGCAC TGGCCAGGCT GTACTGCCGC 
ATGGCCCTGC TCAACATCTT CGCCCCCAGG CTGCCTCACC TGTTTACCCG CCTCTTCCAC 
ATCCCTGCCA TCCGGGACAT TACCCTGGAG CACCTGCAGC TGCTGTCCAA TCAGCTCCTC 
GCTCCTCCCC TCCCAGATGG CACCATCAGC TCCAGCTCCA TCCTCCTGGC ACAGTCGCTG 
CAGCACTGCA TCCATTCCCA GAACTGCTCG GCCACGGACC TCTTCTACCA GGGCAGCTCC 
CAGACAGTGA GAGAGTGGCT CAACGTGGCC ATCACTCGGA CCCTGCACCA GGGTGAGGAG 
AGCCTGTTGG AGCTGACGAA ACAGATCTGC TCTTTCCTGC AGACGGCACC AGAGCAGTTC 
CCCTCCGAAG AGTTTCCAAT TTCCGAGTCC AAGGTCAACA TGGACGTCAA TTTCCCCGGG 
GCGGCTTTCG TCGTGGTATC TTGCAAAGAA AGTCAGTCTG GATTCCGCAA AGACTCCTCT 
CTGTACAAGG CGCCGTGGGC GCGCGTGCTC GTGTACGGAC TGGGCCACAA AGTGAAGCGA 
AATGGCCAGC TAAACCTCAT CGAGGCCATC TGTTACCCGC GGGATGCGTC TCCGGCCAAC 
ACGGGGCTCA CGCCGCCTCC CACCACCAAC CAGTACCCTT CTGTGATCCT CTCCACAGAC 
AAAGTCCACA TCAAACTGGG GGTGTCTCCC CCTCCTGGAG CCGTGCTGGT GTTACACTCT 
CTGCCCCTTG AGTTCCCACT GGCGATGGCC TTCGCAGAGC AGCTGCTGTC CTGGAAGTCG 
GAGGACGGTG AAGGGAGATC TGAAGACGAG CCTGACACCA TCCCGACGTC CGTCCTCCTG 
CAAGTGGTGG AACTGCTCGG AAACTTCCTG TGGACCACGG ACATGGCGGC CTGTGTGAAA 
GAGCTGGTTT TCCACCTCCT GGCAGAGCTC GTGCGCACAG TGCACAGCCT GGAGCAGAGG 
AAGCACCCTG CCGGCCTGTC CTCGTCCATC GCCCTCCAGC TGAACCCCTG CCTGGCCATG 
CTGATGGCCC TGCAGTCAGA GCTCCACAAG CTGTACGACG AGGAGACGCA GAGCTGGGTC 
TCGGGCAGCA CGTGCGGAGG CTCGGGGACC GTGGCGGCCA CCGACCAGGG CAGGTTCTCT 
ACGTATTTCC ACGCGCTCAT GGAAGGGTGC CTGGCGGTGG CCGAGGTGAC CCTGCCTACC 
AACATGAGCG TCACTGCCAG CGGGGTGACG TCGGCGACCG CCCCAAACCT CAGTGACTCG 
TCGTCCTCCT CGTCATCCTC CCCAGGACAG ACGCCGCAGA GCCCCAGCCT CCTGTCCAAG 
AGGAAGAAAG TCAAGATGAA GCGGGAAAAG GCCTCCTCCT CTGGGAAGCG CCAGTCCTCG 
CACTCAGTGG ACTCGGACCC CGCCGTGCTG AGCATCGGGG GTGGCAAGCC CGAAGACATG 
CTGTGGTTCC ATCGCGCCCT GACCTTGCTC ATCATCCTCC GGCACCTCAC CAGGAAGGAC 
CCGCAGGGGC TGGGTGTGAC GAGCGACGCC ATCGCCGACG CCTGCCAGGC CCTGGTGGGT 
CCCACCGCCC ACAGCCGCTT GCTGGTGATC TCTGGGATCC CCACCCACCT GGACGAGGGC 
ATCGTCAGAG GCGCCATCCG CAAGGCCTGC AACGCCCACG GCGGGGTCTT CAAAGACGAG 
ATCTACATCC CCCTGCAGGA CGAAGACCCC AAGAAGCCAA AAGACAAGGC CGAGGGCGGT 
GATGGGAAAG CCGAGCCAGA GAAGACCCTT GGCTTCCCCA GCACAGACAG CTTGGAGGTC 
AGCACGTCCC GCAGCCCGAC CCCCGCCATG AGCATCAGCG CGTCTGCGTC CACCAGCCAA 
GCCTCCATCT GCAGCTCCCA GGGCATCTTG CAGACAGTCA GCGATGTCTC TGTGGAACCG 
CTGCCCTCGG GCCTCGAGCT GCCCATCCCT GCGGGCTTAT TGGAGCCCCA CGTAGTGTCC 
AGCCAGGAGA GCCTGGACGT TTCCCTGTGC AGCACCAGCA GCCTGGGCAG CCTCGGGAGC 
CTGGGGGAGC CACTGGACAA CGCAGAAACG GCCTCAGTGT CAGACATGGG CTCCATGTAC 
ACAGTCACGT CCCTGGACAA CCAGCCCCTC GCAGCACGCC CCATCAAAGG CTTCGCGGTC 
GTGGAGATTA GATCCCGAGC CAAAATCGAG AAAATCCGAG CGAGTTTATT TAACAATAAT 
GACTTGATTG GTTTGTCAAG TTTGGATGGT GAAGATGAAT TGATGGAAAT GTCAACGGAA 
GAGATCCTCA CTGTGTCTGT AGTAAATCAG AGTTTGTTTG ACACGCAGGG GAGCCCAGGG 
TTAGAAGATT ATTTCAATGA TAAGTCAATT AAAGGTGAGA AGCTGGTGCC CGGGGCCAGA 
GAGGTGCTGA CGGAGATATT TAAGAGCTGC GCCCACTCAG AGCAGATGCT GAGTCTGACA 
CCAGCAAAGC CCATCAAAGT GTCTGACATT TATCTCAGCA AAGAGCAGAT CAACTCCCAG 
ACGCCAGGCA ACCTCCTCCA CCTCTTCTTC ACCAATGTTC GGCCCCCCAA AAAGGTGCTG 
GAGGACCAGC TCACCCAGAT CCTGAGGAAG TACGGCGTGC CAAAACCCAA GTTCGACAAG 
AGCAAGTACA GCAAGGCGGG GAAGGAACAG CACCCCGGGA AGGTGGTGAG CACCAAGCGG 
CCCATCACCA AGCCACCTGC CAAGGACAAG GCCGTGCTCA ACAGCGTCAG CAGGACTGCC 
TTGAGCGAGA AGAAGCCCAC TGTGAAGCCG AAGTCACCGG AAAAGAGCAA GCCGGACGAA 
AAGGACCCGG AAAAGTCACC CACCAAAAAA CAAGAAGTCC CTGAGGAAAA GTACCTGACA 
TTGGAAGGAT TTCACAAGTT TGTTATTGAC CGAGCCAAGC AAGACATCCG TAGTGTCTGG 
AGGGCGATTT TATCCTGTGG TTACGATCTT CATTTTGAGA GGTGTGCCTG CATCGACGTC 
CGGCACGCAC ACAAGGCCTC CAGGAAGTGG ACCCTGGAGA TGGACGTGGC GCTCGTGCAG 
TACATCAACC GGCTGTGCCG CCACCTGGCC ATCACGCCCG CGCGGCTGCA TCCCCACGAG 
GTGTATCTGG AGACCACAGA CGCCGCCGAC CCCAGAGTGG CCTGTCTCTT GAATGTGCCC 
ATTGAGAGCC TGCGCCTGCG CTTCGCCCTG CTGCAGTCCC TGAACACCAC GCTCGAGACC 
TTCTTCCTGC CACTCGTGGA GCTGCGCCAG ACGCCCATGT ACACGCACAG CATCGCGGCC 
CTGCTGAAGG AGGCCAAAGG GCTGATCTTT TATGACACGA AGGTGACCGT CATGAATCGG 
GTGCTGAACG CCACAGTGCA GAGGACGGCT GACCACGCGG CACCGGAGAT TACTCTGGAC 
CCACTGGAGA TCGTGGGAGG GGAGATCAGA GCCTCTGAAA ACTCCTACTT CTGCCAGGCG 
GCCAGGCAGC TGGCCTCGGT GCCATCCTCC CAGCTTTGCG TCAGACTGGC GAGTGGCGGT 
GACCCCACCT ATGCCTTCAA CATCCGCTTC ACTGGGGAGG AGGTCCACGG CACCAGCGGC 
TCCTTCCGCC ACTTCCTGTG GCAGGTGTGT AAAGAGCTGC AGAGCTCCGC GCTCTCCCTT 
CTCCTGCTGT GCCCCAGCTC AGCCGTCAAC AAGAACAAGG GCCAGTACAT CCTGAGCCCA 
AGCCCCATCA GCTACGGGGA GGAGCAGCTG CTGCACTTCC TGGGCCAGTT GCTGGGAATT 
GCGATTCGAG CAGATGTCCC TCTTCCCCTG GACCTCCTAC CCTCCTTCTG GAAGACGCTG 
GTGGGCGAGC CCCTGGACCC TGACCAAGAC CTGCAGGAAG CAGACATACT CACCTACAAT 
TATGTCAAGA AATTCGAGAG TATCAACGAC GAGAGCGAGC TGGAGGCCCT GTGCGCCGAG 
ATTGCCTCCC AGCACCTGGC CATCGAGAGC CCCGACGGGC CCAGTAAGCC CTGCTGCCGG 
TTCACCTACC TGACCATGGC GGGCGAGGAG GTGGAGCTGT GCAGCCGCGG CCGGCACATC 
CCTGTGGCGT GGGAGAACAA GGACATCTAC GCAGCAGCCA TCCGGAGCCT GCGGCTGCGG 
GAGCTGCAGA ACGTCGAGTG TGTGACAGCC GTGCGCGCGG GCCTCGGCGC CATCATCCCC 
CTGCAGCTGC TCACCACGCT CAGCCCGCTG GAGATGGAGC TGCGGACGTG CGGCCTCCCT 
TACATCAACC TCGAGTTCCT GAAGGCCCAC ACCATGTACC AGGTGGGCCT GATGGAGACG 
GACCAGCACA TCGAGTTCTT CTGGGGGGCA CTGGAGATGT TTGCCCAGGA GGAGCTGTGC 
AAGTTCATCA AGTTTGCCTG CAACCAAGAG CGCATCCCGT TCACCTGCCC CTGCAAAGAC 
GGGGGCCCCG ACACAGCCCA CGTGCCCCCA TACCCCATGA AGATCGCCCC CCCGGATGGC 
ACAGCAGGTT CCCCAGACTC TCGCTACATC CGAGTGGAGA CCTGCATGTT CATGATCAAG 
CTTCCCCAGT ACTCCTCTCT GGAAATCATG CTGGAGAAAC TTCGTTGTGC CATTCACTAT 
CGCGAAGACC CCCTGAGTGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HECTD4 cDNA ORF clone, Physeter catodon(sperm whale)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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