TET1 cDNA ORF clone, Physeter catodon(sperm whale)

Gene
Clones
gRNAs

Summary

Gene Symbol	TET1
Entrez Gene ID	102988205
Full Name	tet methylcytosine dioxygenase 1
Gene Type	protein-coding
Organism	Physeter catodon(sperm whale)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007121056.1	XP_007121118.1	methylcytosine dioxygenase TET1 isoform X3
XM_024132182.1	XP_023987950.1	methylcytosine dioxygenase TET1 isoform X1
XM_024132182.2	XP_023987950.1	methylcytosine dioxygenase TET1 isoform X1
XM_007121055.1	XP_007121117.1	methylcytosine dioxygenase TET1 isoform X2
XM_007121055.2	XP_007121117.1	methylcytosine dioxygenase TET1 isoform X2

Pathways from BioSystems
Gene Ontology

The following TET1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TET1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPh133400	XM_007121055.2 Latest version!	Physeter catodon tet methylcytosine dioxygenase 1 (TET1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPh133401	XM_007121056.1 Latest version!	Physeter catodon tet methylcytosine dioxygenase 1 (TET1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPh301139	XM_024132182.2 Latest version!	Physeter catodon tet methylcytosine dioxygenase 1 (TET1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OPh301139	XM_024132182.1	Physeter catodon tet methylcytosine dioxygenase 1 (TET1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPh133400	XM_007121055.1	Physeter catodon tet methylcytosine dioxygenase 1 (TET1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TET1 ( XM_007121055.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007121117.1 Physeter catodon tet methylcytosine dioxygenase 1 (TET1), transcript variant X2, mRNA.

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CloneID	OPh133400
Clone ID Related Accession (Same CDS sequence)	XM_007121055.1 , XM_007121055.2
Accession Version	XM_007121055.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	methylcytosine dioxygenase TET1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873592.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGAT CTCGCCATGC AAGGCCTTCC AAATTAGTCA GGAGGGAAGA TCTAAACAAA 
AAGAAAAACA GCCAACTGAA GAAGGGATCT AAGCCAGCCA ACAAAAATGT GGCGACAGTC 
AAGACTGTGA GCCCTGGAAA ATTAAAGCAA TTAATTCAAG AAAGAGGTGT TAAGAAAAAA 
ACAGAACCCA AACTGCCTAT GCCAGTCAGA AGCCTTCTGA CAAGAGCTGG AGCAGCACGA 
ATGAATTTGG ATAGGACTGA GGTTCTTTTT CAGAACCCAG AGTCCTTAAC TTGCAATGGG 
TTTACAATGG CTCTCCGAAG CACCTCTCTT AGCAGACGAC TCTCCCAACC CCCAGCGGTC 
ATAGCCAAAC CCAAGAAAGT TCCACCTCCT AAGAATCTAG GAAAGCAACA TGAGTGTGAT 
TATAAGGTAC TTACTGACGT GGGAGTAAAG CACTCAGAAA GTGATTCAGT CCCAGTGCAA 
GACCCCTCAA TCCCTCCTGA TATAGAGAAT CTAATTGGCA TACAAAATGC ATCATTAATA 
AAAGATGAGA GCCAAGAGAC AACCCAGTCT TGGTCCCAAA GTGTGGAGAA TTCCAAAATA 
AATCGCTCTA TTCATAGTGG CCCTGCAGCA GAGGTTCTTT CTGGGTCATT GGAAGGGACA 
CGTGGTGGTA AAGGACTATT CTCTAGAGAG ACATTGAATG ATATCAGTGA TTCCCCTAGA 
ATGTTTGCTC AGGACACTCT CTGTGTTCCT TTGCTCCAAA AAGCAGCCCT CAGGGTAACC 
TCTGAAGGAA ACCCCAGCAT TCAGTTAGAA GATTTGGGTT CACGAGTAGA ATCTCTTAAG 
CTGTCTGATT CTTACCTGGA TCCCATCAAA AGTGAACATG ATTCCTGCCC CACCTCCAGC 
TTTAATAAGG TTGTACCTGA ATTGGACCTT AGAAACTGTT TGTCTATTGG TGGGTCAATA 
TATCCTACCT CATTAATAAA ACTCCTCTTG GCAGGCTCAG AACAAGTAAC CCTTGGTGCT 
AAACCAGATC ATCAAGAGGT ACTCAAATCT ACCCCAGATG AACAGGAAGT TCCTGATACC 
ACCCCTGTCC TAGGACAGGC CTTTAGTGCT GTCCCACATC AATGGGAAGT TCCTGGTGCT 
AACCCAGTTC ATGGAGAGCC TCTGGGTGAG ACCCCAGGCC CACCAGAGAT TCCTGGTGCT 
ATTCCAGTCC AAGGAGAGGT CTTTGGTGCT CTCTTAAATC AACAAGTCCT CGGTATGGTT 
GGTGGTACTG CCTCAGACCC CGATATCTTT CCTCCTGCAC CTCTTAACCC AATTGCTACC 
TGTAATGCTT TCCCAGAATG GCCTGAGCCC CAGAGCACTG TTTCATCTGG ACTTGAGGTC 
CAGGGCACTA TGCAGATTTT GCCTTCGGGC TTAGGTCACA CTCCTCAACC ATCATCAAAC 
TCAGAGATAA GTTCGGTACC TCCTGCAATG GCTATAAACA ATATAGAAAA TAAGAAGAAG 
GTTCATATAA GCTTTCTGCC AGCTAACACT CAGGGGTTCA CAATAGCCCC TGAGAGAGGA 
CTCCACCATG CTTCACAGGG CATTGTCCAC CTCTCCCAGG CCAGTCCCAG CAAATTGGAA 
GGAGGGAGCT CCCAGATCAG TGCCACCAGC ATGGTTGACA TCAACACCAC AGTGGTGTCT 
AGGCCAGTGT CACTTGTCAG TACCTCCTCT TCTCCTTCCT ACACAACGTT GCTACCTACT 
TTGGAAAAGA AGAAACGAAA GCGATGTGGG GTCTGTGAGC CCTGCCAGCA GAAGACCAAT 
TGTGGTGAAT GTACCTACTG CAAGAATAGA AAGAACAGCC ATCAGATCTG TAAGAAGAGG 
AAATGTGAGG AACTGAAAAA GAAGCCATCC ATCATTGTGC CTTTGGAGGC AGATTTTGAC 
AACAAACCAG TAAATGGCCC CAAGTCAGAA TCCATGGAAT ACAGTAGTTG TGGTCATGAG 
GAAGAAAAAA GATTGGAATT AAATTCATGT CCCTTAGAAA ATGTAACTAA AAATGAAGAA 
AGTATGACAG GTATTGAAGT GGAAAAGTGG ACACAAAACA AGAAATCACA CTTAACTGAT 
CATGTTAAAG GAGAATTTAA TACAATTAAA ACAGAAGCTG AAAATTTGAA AAGCTCTGAA 
GATGACAGGA AAAAAATCCT ACTTACTGAC CTCCTTGAAC CTCAAAAATT GTTTGCACAA 
ACTGTAAGAA ACGGCATTAA AAATGTACAC TATTTGCCAA CTGAAACAAA TTTGTCTTTT 
AAAAAAATCA ACATTGAAGA ACTTGGCAAG GCATTTGAAA ACAATCCTTA TAAATTCCTG 
AAAGACACTG CAAACCATAA CAATGCCATG AGCGCCATTG CTACTAGTGC GAGCTGTGAT 
CATCTCAAGG GGAGAAGTAA TGTTTTGGTC ATTCAGAAGC CTGGCTTTAA TTGTAAGTCT 
TTTCCAGATC CTGCAAACTG CAACTTAAAT AGTCATCCTA GTATACACAA TGAAAGTGAT 
CAACCAAAAT CCCTAGAAAT TATAACAAAT AGAGAACTAA AAGAAGGATC AACACTTCAA 
CCAAGTCTTC TATCCCTAAT GAAAGATAGG AGATTAACGC TAGAACAAGT AGTGGCCATA 
GAAGCTCTAA CACAACTTTC AGAAGCCCCA TCAGAAAATT CCTCACCGTC GAAGTCAGAG 
AAGGATGAAG AAACACAACA GAGAACAGCT AGTTTGCTTA ACAGCTGTAA AGCTATCCTG 
TATTCTGTAA GAAAAGACCT CCAAGACCCA AATTTACAAG GGGGGCCACA AAGCCTTCAT 
CACTGTCCAT CTCTGGAAAA GCAAAGTTCA TGCAACACGT TGATGTTCAA TGGCCAAAAT 
ATTTCTAAGT CACACAACAG CTCATCTAGT GACCAAGCAT CCACAAAGGC ACAGGAATAT 
TCAATAGTCA CAAATTCAGT ATCTGTTTTT ATACCAAATT CAAATTCATC TAAAACCGAT 
ACCAATAAAA ATGTTGCTCA AGGTAGAACT GCTCTTGACA GTTCCAAAAA TTTGCATCAG 
TTGACAACAA CAACTAGTAA GTCAGAGTAT TGTAACCAGT CACTGGACAG CAGCATCAAG 
TTGGACTCAA AGGATGATCC CTCATGTCAA GATACAGTTC ATAGTAAAAT AGAAGAAGAT 
GTTGCTGCAC AGTTAACACA ACTTGCATCA ATAATTAAAT TCAATTGTAT AAAACCGGAG 
GACAAAAAAG TTGAAAGTAC ACCAACAAGC CTTGTTGCAT ATAATGTACA GCAAAACTAT 
AGCCAGGAGA AGGGCACAAT AGAGCATAAA CCACCTTCCA GTGTACAAAA TAATCAAGGA 
CCTTCAGTAA CAAAGCAAAA GAACACAATC CAGAAAAAGA CAAAATCTAC CCCATCCAGA 
GATCGACGGA AAAAGAAGCC TGTGATCATA AGTTGTCAAG AAAATGACCA GAAAAAACAG 
GAACAGTTGG CATATGAATA TAGTAAGTTA CATGACATTT GGATAGCATC TAAATTTCAA 
AGATTTGGTC AGTTTGGTCC ACATGACTTT CCTATTCTAT TGGGAAAAAT TCCTCCCCTA 
ACCAAAGTAT GGAAACCACT GACTCAAAGG AGCTCAGCAT TAGAACACAA AAAATTATTT 
CCTCCACTCA CTCAAATAAA ATTTGAGCGG TATTATCCTG AATTAGCCCA GGAGAAGATG 
AAGGTTGAAC CCTTGGATTC TCTCCCCATA ATCCAGTTAA AAGCAGAATC CAACGGACAG 
GCGTTTACAG AAAATGGATA TGCTCAGGTA CAGCCAACAG TGAATGTCAA TCAGAAAGCT 
CATCCTTTAC TCCGGCCAAC CTCTCCAACT AACCAGTGTG CTAATGGGAT GGCTGGCAAT 
GACCAAACAC AGTTCCAACA GGATGTTAAA GACCAAGTCA CACATCAGAG GCTGCCGACA 
TTGCCTGGTA TCTCTCATGA GACCCCTTTA CCGGACCCAG CACAAATTCT CAGGAACCTA 
AATGTAGTAT GTTCAGGTGG AATTACAGTG GTTTCTACCA AAAGTGAAGA GGACGTCTGT 
TCATCTAGTG TTGGAGCATC AGAATTTTCC CCAATAGACA GTGCGCAGAA AAGTTTTAAT 
GATTATGCGA TGAACTTCTT TACTAATCCT ACGAAAACCC TGGTGTCCAC AACAAAAGAT 
TCTGAATTGC CTACCTGCAA CTGTCTTGAT CGAGTTATAC AAAAAGACAA AGGCCCATAT 
TATACACACC TTGGGGCAGG ACCAAGTGTT GCTGCTGTCA GGGAAATCAT GGAGAATAGG 
TATGGTCAAA AAGGAAACGC AATAAGGATA GAAATAGTAG TGTACACGGG GAAAGAAGGA 
AAAAGCTCTA ATGGGTGTCC AGTTGCTAAG TGGGTTTTAA GAAGAAGCAG CGATGAAGAG 
AAAGTTCTTT GTCTCGTTCG ACAGCGTGCA GGCCATCACT GTCCAACAGC TGTGATGGTG 
GTGCTCATCA TGGTATGGGA CGGCATCCCT CTTCCAATGG CTGACAAATT GTACTCGCAG 
CTTACGGAAA GTCTAAAGTC ATACAATGGT CATCCCACAG ATCGAAGATG CACTCTCAAT 
GAAAATCGTA CCTGTACATG TCAAGGAATT GATCCAGAGA CTTGCGGAGC TTCCTTCTCT 
TTTGGCTGTT CTTGGAGTAT GTATTTCAAT GGCTGCAAAT TTGGTAGAAG CCCAAGTCCT 
AGAAGATTTA GAATTGATCC AAGTTCTCCC TTACACCACT ACTATGAAAG AATTACTAAA 
GGACGTAATC CAGAAAGAAG ATATATGAAA CCGGAACCAA TCTGTCCGGA ACACGAGGCC 
ATGGAAAAAA ACCTTGAAGA TAATTTACAG AGTTTGGCTA CAGAATTAGC TCCAATTTAT 
AAGCAGTATG CTCCAGTTGC TTACCAAAAC CAGGTGGCGC TTGAACACGT TGCTCAAGAA 
TGTCGGCTTG GCAAGAAAGA AGGTCGTCCT TTCTCTGGGG TCACTGCTTG CCTAGACTTC 
TGTGCCCACC CCCACAGGGA CATTCACAAC ATGAATAATG GGAGCACGGT GGTCTGCACT 
TTAACACGAG AAGATAACCG CTCATTTGGT GTTATTCCTC AAGATGAGCA GCTGCATGTA 
CTTCCTCTTT ATAAACTTTC AGACACAGAT GAGTTTGGCT CAAGGGACGG AATGGAAGCC 
AAGATCAAAT CTGGGGCCAT CATGGTCCTC GCACCCCACC GCAGAAAAAG AACACGATTT 
ACTCAGCCAG TTCCTCGTTC TGGGAAGAAG AGAGCAGCAA TGATGACAGA GGTTCTCGCA 
CATAAGATAA GGGCTGTGGA AAAGAAATTT ATTCCTCGAA TCAAGCGGAA GAATAATTCA 
ATGACAAACA ACAGTAAGGC TTCATCGCTG CCACTTTTAG GGAATAAAAC AGAGACTGTG 
CAACCTGAAA TAAAAAGTGA AACTGAACCC CACCTTATCT TCAAAGGTTC GGACAACACT 
AAAACCTACT CACCGACCCC ATCCATTCCT CACCCAGTGA AAGAGGCACA CTTAGCTCCA 
GGCTTCTCCT GGTCCCCTAA GACTGCTTCG GTCCCAACAG TTCCTTTTAA GAATGATGCA 
TCAGTTTCGT ACGGGTTTTC AGAAAGAGGT AGCAATCCCC ACTGCACAAT GCCTTCTGCA 
AGACACAGTG GTGCTAATGT AGCTGCTGGG GAGTGTGCTG AAACTGCACA GCCTGGTGAA 
ATGGTTCCTC TTTCCACCTT GTCTACTCCC ATGACAGATT CCCTGGCTTA TTCTGAGCCT 
CCCACTGGTC CATCTGAGCA GCTAACTTCT GATCAGCCAA ACCAGCAGCC CCCATTCATC 
CCTTCTCCTC ATGAACTTGC TTCTTCTCTG GCGGAAGAAG ACGAGCAGCA TTCTGAAGCA 
GATGAGCCTC CATCAGATGA CCCCCTATCA GACGACCCCC TGTCACCTGC TGAGGAGAAA 
CTGCCCCACA TTGTTGAGTA TTGGTCAGAC AGTGAGCACA TCTTCCTGGA TGCCAATGTT 
GGTGGGGTGG CCATAGCGCC CGCCCACGGC TCTGTTCTGA TCGAGTGCGC CAGGCGAGAG 
CTTCACGCCA CAACTCCTGT CGAACACCCC AACCGGAATC ACCCTACACG CCTGTCTCTT 
GTCTTCTACC AGCACAAAAA CCTGAATAAG CCCCAACACG GTTTCGAACT AAACAAGATT 
AAGTTCGAGG CGAAAGAAGC AAAGAATAAG AAAACTAAAG CCTCCGAGCA GAAAGACCAG 
GCAGCTAACG AGGGTCCTGA ACTGTCCACT GAAGTTAATG AATTTAACCA GATTCCTTCC 
CACAAAGCGT TAACATTAAC CCATGACAAT GTTGTCACCG TGTCCCCTTA TGCTCTCACA 
CATGTTGCGG GGCCCTATAA CCATTGGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007121117.1
CDS	1..6453
Translation	MSRSRHARPSKLVRREDLNKKKNSQLKKGSKPANKNVATVKTVSPGKLKQLIQERGVKKKTEPKLPMPVRSLLTRAGAARMNLDRTEVLFQNPESLTCNGFTMALRSTSLSRRLSQPPAVIAKPKKVPPPKNLGKQHECDYKVLTDVGVKHSESDSVPVQDPSIPPDIENLIGIQNASLIKDESQETTQSWSQSVENSKINRSIHSGPAAEVLSGSLEGTRGGKGLFSRETLNDISDSPRMFAQDTLCVPLLQKAALRVTSEGNPSIQLEDLGSRVESLKLSDSYLDPIKSEHDSCPTSSFNKVVPELDLRNCLSIGGSIYPTSLIKLLLAGSEQVTLGAKPDHQEVLKSTPDEQEVPDTTPVLGQAFSAVPHQWEVPGANPVHGEPLGETPGPPEIPGAIPVQGEVFGALLNQQVLGMVGGTASDPDIFPPAPLNPIATCNAFPEWPEPQSTVSSGLEVQGTMQILPSGLGHTPQPSSNSEISSVPPAMAINNIENKKKVHISFLPANTQGFTIAPERGLHHASQGIVHLSQASPSKLEGGSSQISATSMVDINTTVVSRPVSLVSTSSSPSYTTLLPTLEKKKRKRCGVCEPCQQKTNCGECTYCKNRKNSHQICKKRKCEELKKKPSIIVPLEADFDNKPVNGPKSESMEYSSCGHEEEKRLELNSCPLENVTKNEESMTGIEVEKWTQNKKSHLTDHVKGEFNTIKTEAENLKSSEDDRKKILLTDLLEPQKLFAQTVRNGIKNVHYLPTETNLSFKKINIEELGKAFENNPYKFLKDTANHNNAMSAIATSASCDHLKGRSNVLVIQKPGFNCKSFPDPANCNLNSHPSIHNESDQPKSLEIITNRELKEGSTLQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSENSSPSKSEKDEETQQRTASLLNSCKAILYSVRKDLQDPNLQGGPQSLHHCPSLEKQSSCNTLMFNGQNISKSHNSSSSDQASTKAQEYSIVTNSVSVFIPNSNSSKTDTNKNVAQGRTALDSSKNLHQLTTTTSKSEYCNQSLDSSIKLDSKDDPSCQDTVHSKIEEDVAAQLTQLASIIKFNCIKPEDKKVESTPTSLVAYNVQQNYSQEKGTIEHKPPSSVQNNQGPSVTKQKNTIQKKTKSTPSRDRRKKKPVIISCQENDQKKQEQLAYEYSKLHDIWIASKFQRFGQFGPHDFPILLGKIPPLTKVWKPLTQRSSALEHKKLFPPLTQIKFERYYPELAQEKMKVEPLDSLPIIQLKAESNGQAFTENGYAQVQPTVNVNQKAHPLLRPTSPTNQCANGMAGNDQTQFQQDVKDQVTHQRLPTLPGISHETPLPDPAQILRNLNVVCSGGITVVSTKSEEDVCSSSVGASEFSPIDSAQKSFNDYAMNFFTNPTKTLVSTTKDSELPTCNCLDRVIQKDKGPYYTHLGAGPSVAAVREIMENRYGQKGNAIRIEIVVYTGKEGKSSNGCPVAKWVLRRSSDEEKVLCLVRQRAGHHCPTAVMVVLIMVWDGIPLPMADKLYSQLTESLKSYNGHPTDRRCTLNENRTCTCQGIDPETCGASFSFGCSWSMYFNGCKFGRSPSPRRFRIDPSSPLHHYYERITKGRNPERRYMKPEPICPEHEAMEKNLEDNLQSLATELAPIYKQYAPVAYQNQVALEHVAQECRLGKKEGRPFSGVTACLDFCAHPHRDIHNMNNGSTVVCTLTREDNRSFGVIPQDEQLHVLPLYKLSDTDEFGSRDGMEAKIKSGAIMVLAPHRRKRTRFTQPVPRSGKKRAAMMTEVLAHKIRAVEKKFIPRIKRKNNSMTNNSKASSLPLLGNKTETVQPEIKSETEPHLIFKGSDNTKTYSPTPSIPHPVKEAHLAPGFSWSPKTASVPTVPFKNDASVSYGFSERGSNPHCTMPSARHSGANVAAGECAETAQPGEMVPLSTLSTPMTDSLAYSEPPTGPSEQLTSDQPNQQPPFIPSPHELASSLAEEDEQHSEADEPPSDDPLSDDPLSPAEEKLPHIVEYWSDSEHIFLDANVGGVAIAPAHGSVLIECARRELHATTPVEHPNRNHPTRLSLVFYQHKNLNKPQHGFELNKIKFEAKEAKNKKTKASEQKDQAANEGPELSTEVNEFNQIPSHKALTLTHDNVVTVSPYALTHVAGPYNHWV

Target ORF information:

RefSeq Version	XM_007121055.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon tet methylcytosine dioxygenase 1 (TET1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007121055.1

ORF Insert Sequence:

ATGTCTCGAT CTCGCCATGC AAGGCCTTCC AAATTAGTCA GGAGGGAAGA TCTAAACAAA 
AAGAAAAACA GCCAACTGAA GAAGGGATCT AAGCCAGCCA ACAAAAATGT GGCGACAGTC 
AAGACTGTGA GCCCTGGAAA ATTAAAGCAA TTAATTCAAG AAAGAGGTGT TAAGAAAAAA 
ACAGAACCCA AACTGCCTAT GCCAGTCAGA AGCCTTCTGA CAAGAGCTGG AGCAGCACGA 
ATGAATTTGG ATAGGACTGA GGTTCTTTTT CAGAACCCAG AGTCCTTAAC TTGCAATGGG 
TTTACAATGG CTCTCCGAAG CACCTCTCTT AGCAGACGAC TCTCCCAACC CCCAGCGGTC 
ATAGCCAAAC CCAAGAAAGT TCCACCTCCT AAGAATCTAG GAAAGCAACA TGAGTGTGAT 
TATAAGGTAC TTACTGACGT GGGAGTAAAG CACTCAGAAA GTGATTCAGT CCCAGTGCAA 
GACCCCTCAA TCCCTCCTGA TATAGAGAAT CTAATTGGCA TACAAAATGC ATCATTAATA 
AAAGATGAGA GCCAAGAGAC AACCCAGTCT TGGTCCCAAA GTGTGGAGAA TTCCAAAATA 
AATCGCTCTA TTCATAGTGG CCCTGCAGCA GAGGTTCTTT CTGGGTCATT GGAAGGGACA 
CGTGGTGGTA AAGGACTATT CTCTAGAGAG ACATTGAATG ATATCAGTGA TTCCCCTAGA 
ATGTTTGCTC AGGACACTCT CTGTGTTCCT TTGCTCCAAA AAGCAGCCCT CAGGGTAACC 
TCTGAAGGAA ACCCCAGCAT TCAGTTAGAA GATTTGGGTT CACGAGTAGA ATCTCTTAAG 
CTGTCTGATT CTTACCTGGA TCCCATCAAA AGTGAACATG ATTCCTGCCC CACCTCCAGC 
TTTAATAAGG TTGTACCTGA ATTGGACCTT AGAAACTGTT TGTCTATTGG TGGGTCAATA 
TATCCTACCT CATTAATAAA ACTCCTCTTG GCAGGCTCAG AACAAGTAAC CCTTGGTGCT 
AAACCAGATC ATCAAGAGGT ACTCAAATCT ACCCCAGATG AACAGGAAGT TCCTGATACC 
ACCCCTGTCC TAGGACAGGC CTTTAGTGCT GTCCCACATC AATGGGAAGT TCCTGGTGCT 
AACCCAGTTC ATGGAGAGCC TCTGGGTGAG ACCCCAGGCC CACCAGAGAT TCCTGGTGCT 
ATTCCAGTCC AAGGAGAGGT CTTTGGTGCT CTCTTAAATC AACAAGTCCT CGGTATGGTT 
GGTGGTACTG CCTCAGACCC CGATATCTTT CCTCCTGCAC CTCTTAACCC AATTGCTACC 
TGTAATGCTT TCCCAGAATG GCCTGAGCCC CAGAGCACTG TTTCATCTGG ACTTGAGGTC 
CAGGGCACTA TGCAGATTTT GCCTTCGGGC TTAGGTCACA CTCCTCAACC ATCATCAAAC 
TCAGAGATAA GTTCGGTACC TCCTGCAATG GCTATAAACA ATATAGAAAA TAAGAAGAAG 
GTTCATATAA GCTTTCTGCC AGCTAACACT CAGGGGTTCA CAATAGCCCC TGAGAGAGGA 
CTCCACCATG CTTCACAGGG CATTGTCCAC CTCTCCCAGG CCAGTCCCAG CAAATTGGAA 
GGAGGGAGCT CCCAGATCAG TGCCACCAGC ATGGTTGACA TCAACACCAC AGTGGTGTCT 
AGGCCAGTGT CACTTGTCAG TACCTCCTCT TCTCCTTCCT ACACAACGTT GCTACCTACT 
TTGGAAAAGA AGAAACGAAA GCGATGTGGG GTCTGTGAGC CCTGCCAGCA GAAGACCAAT 
TGTGGTGAAT GTACCTACTG CAAGAATAGA AAGAACAGCC ATCAGATCTG TAAGAAGAGG 
AAATGTGAGG AACTGAAAAA GAAGCCATCC ATCATTGTGC CTTTGGAGGC AGATTTTGAC 
AACAAACCAG TAAATGGCCC CAAGTCAGAA TCCATGGAAT ACAGTAGTTG TGGTCATGAG 
GAAGAAAAAA GATTGGAATT AAATTCATGT CCCTTAGAAA ATGTAACTAA AAATGAAGAA 
AGTATGACAG GTATTGAAGT GGAAAAGTGG ACACAAAACA AGAAATCACA CTTAACTGAT 
CATGTTAAAG GAGAATTTAA TACAATTAAA ACAGAAGCTG AAAATTTGAA AAGCTCTGAA 
GATGACAGGA AAAAAATCCT ACTTACTGAC CTCCTTGAAC CTCAAAAATT GTTTGCACAA 
ACTGTAAGAA ACGGCATTAA AAATGTACAC TATTTGCCAA CTGAAACAAA TTTGTCTTTT 
AAAAAAATCA ACATTGAAGA ACTTGGCAAG GCATTTGAAA ACAATCCTTA TAAATTCCTG 
AAAGACACTG CAAACCATAA CAATGCCATG AGCGCCATTG CTACTAGTGC GAGCTGTGAT 
CATCTCAAGG GGAGAAGTAA TGTTTTGGTC ATTCAGAAGC CTGGCTTTAA TTGTAAGTCT 
TTTCCAGATC CTGCAAACTG CAACTTAAAT AGTCATCCTA GTATACACAA TGAAAGTGAT 
CAACCAAAAT CCCTAGAAAT TATAACAAAT AGAGAACTAA AAGAAGGATC AACACTTCAA 
CCAAGTCTTC TATCCCTAAT GAAAGATAGG AGATTAACGC TAGAACAAGT AGTGGCCATA 
GAAGCTCTAA CACAACTTTC AGAAGCCCCA TCAGAAAATT CCTCACCGTC GAAGTCAGAG 
AAGGATGAAG AAACACAACA GAGAACAGCT AGTTTGCTTA ACAGCTGTAA AGCTATCCTG 
TATTCTGTAA GAAAAGACCT CCAAGACCCA AATTTACAAG GGGGGCCACA AAGCCTTCAT 
CACTGTCCAT CTCTGGAAAA GCAAAGTTCA TGCAACACGT TGATGTTCAA TGGCCAAAAT 
ATTTCTAAGT CACACAACAG CTCATCTAGT GACCAAGCAT CCACAAAGGC ACAGGAATAT 
TCAATAGTCA CAAATTCAGT ATCTGTTTTT ATACCAAATT CAAATTCATC TAAAACCGAT 
ACCAATAAAA ATGTTGCTCA AGGTAGAACT GCTCTTGACA GTTCCAAAAA TTTGCATCAG 
TTGACAACAA CAACTAGTAA GTCAGAGTAT TGTAACCAGT CACTGGACAG CAGCATCAAG 
TTGGACTCAA AGGATGATCC CTCATGTCAA GATACAGTTC ATAGTAAAAT AGAAGAAGAT 
GTTGCTGCAC AGTTAACACA ACTTGCATCA ATAATTAAAT TCAATTGTAT AAAACCGGAG 
GACAAAAAAG TTGAAAGTAC ACCAACAAGC CTTGTTGCAT ATAATGTACA GCAAAACTAT 
AGCCAGGAGA AGGGCACAAT AGAGCATAAA CCACCTTCCA GTGTACAAAA TAATCAAGGA 
CCTTCAGTAA CAAAGCAAAA GAACACAATC CAGAAAAAGA CAAAATCTAC CCCATCCAGA 
GATCGACGGA AAAAGAAGCC TGTGATCATA AGTTGTCAAG AAAATGACCA GAAAAAACAG 
GAACAGTTGG CATATGAATA TAGTAAGTTA CATGACATTT GGATAGCATC TAAATTTCAA 
AGATTTGGTC AGTTTGGTCC ACATGACTTT CCTATTCTAT TGGGAAAAAT TCCTCCCCTA 
ACCAAAGTAT GGAAACCACT GACTCAAAGG AGCTCAGCAT TAGAACACAA AAAATTATTT 
CCTCCACTCA CTCAAATAAA ATTTGAGCGG TATTATCCTG AATTAGCCCA GGAGAAGATG 
AAGGTTGAAC CCTTGGATTC TCTCCCCATA ATCCAGTTAA AAGCAGAATC CAACGGACAG 
GCGTTTACAG AAAATGGATA TGCTCAGGTA CAGCCAACAG TGAATGTCAA TCAGAAAGCT 
CATCCTTTAC TCCGGCCAAC CTCTCCAACT AACCAGTGTG CTAATGGGAT GGCTGGCAAT 
GACCAAACAC AGTTCCAACA GGATGTTAAA GACCAAGTCA CACATCAGAG GCTGCCGACA 
TTGCCTGGTA TCTCTCATGA GACCCCTTTA CCGGACCCAG CACAAATTCT CAGGAACCTA 
AATGTAGTAT GTTCAGGTGG AATTACAGTG GTTTCTACCA AAAGTGAAGA GGACGTCTGT 
TCATCTAGTG TTGGAGCATC AGAATTTTCC CCAATAGACA GTGCGCAGAA AAGTTTTAAT 
GATTATGCGA TGAACTTCTT TACTAATCCT ACGAAAACCC TGGTGTCCAC AACAAAAGAT 
TCTGAATTGC CTACCTGCAA CTGTCTTGAT CGAGTTATAC AAAAAGACAA AGGCCCATAT 
TATACACACC TTGGGGCAGG ACCAAGTGTT GCTGCTGTCA GGGAAATCAT GGAGAATAGG 
TATGGTCAAA AAGGAAACGC AATAAGGATA GAAATAGTAG TGTACACGGG GAAAGAAGGA 
AAAAGCTCTA ATGGGTGTCC AGTTGCTAAG TGGGTTTTAA GAAGAAGCAG CGATGAAGAG 
AAAGTTCTTT GTCTCGTTCG ACAGCGTGCA GGCCATCACT GTCCAACAGC TGTGATGGTG 
GTGCTCATCA TGGTATGGGA CGGCATCCCT CTTCCAATGG CTGACAAATT GTACTCGCAG 
CTTACGGAAA GTCTAAAGTC ATACAATGGT CATCCCACAG ATCGAAGATG CACTCTCAAT 
GAAAATCGTA CCTGTACATG TCAAGGAATT GATCCAGAGA CTTGCGGAGC TTCCTTCTCT 
TTTGGCTGTT CTTGGAGTAT GTATTTCAAT GGCTGCAAAT TTGGTAGAAG CCCAAGTCCT 
AGAAGATTTA GAATTGATCC AAGTTCTCCC TTACACCACT ACTATGAAAG AATTACTAAA 
GGACGTAATC CAGAAAGAAG ATATATGAAA CCGGAACCAA TCTGTCCGGA ACACGAGGCC 
ATGGAAAAAA ACCTTGAAGA TAATTTACAG AGTTTGGCTA CAGAATTAGC TCCAATTTAT 
AAGCAGTATG CTCCAGTTGC TTACCAAAAC CAGGTGGCGC TTGAACACGT TGCTCAAGAA 
TGTCGGCTTG GCAAGAAAGA AGGTCGTCCT TTCTCTGGGG TCACTGCTTG CCTAGACTTC 
TGTGCCCACC CCCACAGGGA CATTCACAAC ATGAATAATG GGAGCACGGT GGTCTGCACT 
TTAACACGAG AAGATAACCG CTCATTTGGT GTTATTCCTC AAGATGAGCA GCTGCATGTA 
CTTCCTCTTT ATAAACTTTC AGACACAGAT GAGTTTGGCT CAAGGGACGG AATGGAAGCC 
AAGATCAAAT CTGGGGCCAT CATGGTCCTC GCACCCCACC GCAGAAAAAG AACACGATTT 
ACTCAGCCAG TTCCTCGTTC TGGGAAGAAG AGAGCAGCAA TGATGACAGA GGTTCTCGCA 
CATAAGATAA GGGCTGTGGA AAAGAAATTT ATTCCTCGAA TCAAGCGGAA GAATAATTCA 
ATGACAAACA ACAGTAAGGC TTCATCGCTG CCACTTTTAG GGAATAAAAC AGAGACTGTG 
CAACCTGAAA TAAAAAGTGA AACTGAACCC CACCTTATCT TCAAAGGTTC GGACAACACT 
AAAACCTACT CACCGACCCC ATCCATTCCT CACCCAGTGA AAGAGGCACA CTTAGCTCCA 
GGCTTCTCCT GGTCCCCTAA GACTGCTTCG GTCCCAACAG TTCCTTTTAA GAATGATGCA 
TCAGTTTCGT ACGGGTTTTC AGAAAGAGGT AGCAATCCCC ACTGCACAAT GCCTTCTGCA 
AGACACAGTG GTGCTAATGT AGCTGCTGGG GAGTGTGCTG AAACTGCACA GCCTGGTGAA 
ATGGTTCCTC TTTCCACCTT GTCTACTCCC ATGACAGATT CCCTGGCTTA TTCTGAGCCT 
CCCACTGGTC CATCTGAGCA GCTAACTTCT GATCAGCCAA ACCAGCAGCC CCCATTCATC 
CCTTCTCCTC ATGAACTTGC TTCTTCTCTG GCGGAAGAAG ACGAGCAGCA TTCTGAAGCA 
GATGAGCCTC CATCAGATGA CCCCCTATCA GACGACCCCC TGTCACCTGC TGAGGAGAAA 
CTGCCCCACA TTGTTGAGTA TTGGTCAGAC AGTGAGCACA TCTTCCTGGA TGCCAATGTT 
GGTGGGGTGG CCATAGCGCC CGCCCACGGC TCTGTTCTGA TCGAGTGCGC CAGGCGAGAG 
CTTCACGCCA CAACTCCTGT CGAACACCCC AACCGGAATC ACCCTACACG CCTGTCTCTT 
GTCTTCTACC AGCACAAAAA CCTGAATAAG CCCCAACACG GTTTCGAACT AAACAAGATT 
AAGTTCGAGG CGAAAGAAGC AAAGAATAAG AAAACTAAAG CCTCCGAGCA GAAAGACCAG 
GCAGCTAACG AGGGTCCTGA ACTGTCCACT GAAGTTAATG AATTTAACCA GATTCCTTCC 
CACAAAGCGT TAACATTAAC CCATGACAAT GTTGTCACCG TGTCCCCTTA TGCTCTCACA 
CATGTTGCGG GGCCCTATAA CCATTGGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TET1 ( XM_007121055.2 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007121117.1 Physeter catodon tet methylcytosine dioxygenase 1 (TET1), transcript variant X2, mRNA.

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CloneID	OPh133400
Clone ID Related Accession (Same CDS sequence)	XM_007121055.1 , XM_007121055.2
Accession Version	XM_007121055.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	methylcytosine dioxygenase TET1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041233.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2019 this sequence version replaced XM_007121055.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGAT CTCGCCATGC AAGGCCTTCC AAATTAGTCA GGAGGGAAGA TCTAAACAAA 
AAGAAAAACA GCCAACTGAA GAAGGGATCT AAGCCAGCCA ACAAAAATGT GGCGACAGTC 
AAGACTGTGA GCCCTGGAAA ATTAAAGCAA TTAATTCAAG AAAGAGGTGT TAAGAAAAAA 
ACAGAACCCA AACTGCCTAT GCCAGTCAGA AGCCTTCTGA CAAGAGCTGG AGCAGCACGA 
ATGAATTTGG ATAGGACTGA GGTTCTTTTT CAGAACCCAG AGTCCTTAAC TTGCAATGGG 
TTTACAATGG CTCTCCGAAG CACCTCTCTT AGCAGACGAC TCTCCCAACC CCCAGCGGTC 
ATAGCCAAAC CCAAGAAAGT TCCACCTCCT AAGAATCTAG GAAAGCAACA TGAGTGTGAT 
TATAAGGTAC TTACTGACGT GGGAGTAAAG CACTCAGAAA GTGATTCAGT CCCAGTGCAA 
GACCCCTCAA TCCCTCCTGA TATAGAGAAT CTAATTGGCA TACAAAATGC ATCATTAATA 
AAAGATGAGA GCCAAGAGAC AACCCAGTCT TGGTCCCAAA GTGTGGAGAA TTCCAAAATA 
AATCGCTCTA TTCATAGTGG CCCTGCAGCA GAGGTTCTTT CTGGGTCATT GGAAGGGACA 
CGTGGTGGTA AAGGACTATT CTCTAGAGAG ACATTGAATG ATATCAGTGA TTCCCCTAGA 
ATGTTTGCTC AGGACACTCT CTGTGTTCCT TTGCTCCAAA AAGCAGCCCT CAGGGTAACC 
TCTGAAGGAA ACCCCAGCAT TCAGTTAGAA GATTTGGGTT CACGAGTAGA ATCTCTTAAG 
CTGTCTGATT CTTACCTGGA TCCCATCAAA AGTGAACATG ATTCCTGCCC CACCTCCAGC 
TTTAATAAGG TTGTACCTGA ATTGGACCTT AGAAACTGTT TGTCTATTGG TGGGTCAATA 
TATCCTACCT CATTAATAAA ACTCCTCTTG GCAGGCTCAG AACAAGTAAC CCTTGGTGCT 
AAACCAGATC ATCAAGAGGT ACTCAAATCT ACCCCAGATG AACAGGAAGT TCCTGATACC 
ACCCCTGTCC TAGGACAGGC CTTTAGTGCT GTCCCACATC AATGGGAAGT TCCTGGTGCT 
AACCCAGTTC ATGGAGAGCC TCTGGGTGAG ACCCCAGGCC CACCAGAGAT TCCTGGTGCT 
ATTCCAGTCC AAGGAGAGGT CTTTGGTGCT CTCTTAAATC AACAAGTCCT CGGTATGGTT 
GGTGGTACTG CCTCAGACCC CGATATCTTT CCTCCTGCAC CTCTTAACCC AATTGCTACC 
TGTAATGCTT TCCCAGAATG GCCTGAGCCC CAGAGCACTG TTTCATCTGG ACTTGAGGTC 
CAGGGCACTA TGCAGATTTT GCCTTCGGGC TTAGGTCACA CTCCTCAACC ATCATCAAAC 
TCAGAGATAA GTTCGGTACC TCCTGCAATG GCTATAAACA ATATAGAAAA TAAGAAGAAG 
GTTCATATAA GCTTTCTGCC AGCTAACACT CAGGGGTTCA CAATAGCCCC TGAGAGAGGA 
CTCCACCATG CTTCACAGGG CATTGTCCAC CTCTCCCAGG CCAGTCCCAG CAAATTGGAA 
GGAGGGAGCT CCCAGATCAG TGCCACCAGC ATGGTTGACA TCAACACCAC AGTGGTGTCT 
AGGCCAGTGT CACTTGTCAG TACCTCCTCT TCTCCTTCCT ACACAACGTT GCTACCTACT 
TTGGAAAAGA AGAAACGAAA GCGATGTGGG GTCTGTGAGC CCTGCCAGCA GAAGACCAAT 
TGTGGTGAAT GTACCTACTG CAAGAATAGA AAGAACAGCC ATCAGATCTG TAAGAAGAGG 
AAATGTGAGG AACTGAAAAA GAAGCCATCC ATCATTGTGC CTTTGGAGGC AGATTTTGAC 
AACAAACCAG TAAATGGCCC CAAGTCAGAA TCCATGGAAT ACAGTAGTTG TGGTCATGAG 
GAAGAAAAAA GATTGGAATT AAATTCATGT CCCTTAGAAA ATGTAACTAA AAATGAAGAA 
AGTATGACAG GTATTGAAGT GGAAAAGTGG ACACAAAACA AGAAATCACA CTTAACTGAT 
CATGTTAAAG GAGAATTTAA TACAATTAAA ACAGAAGCTG AAAATTTGAA AAGCTCTGAA 
GATGACAGGA AAAAAATCCT ACTTACTGAC CTCCTTGAAC CTCAAAAATT GTTTGCACAA 
ACTGTAAGAA ACGGCATTAA AAATGTACAC TATTTGCCAA CTGAAACAAA TTTGTCTTTT 
AAAAAAATCA ACATTGAAGA ACTTGGCAAG GCATTTGAAA ACAATCCTTA TAAATTCCTG 
AAAGACACTG CAAACCATAA CAATGCCATG AGCGCCATTG CTACTAGTGC GAGCTGTGAT 
CATCTCAAGG GGAGAAGTAA TGTTTTGGTC ATTCAGAAGC CTGGCTTTAA TTGTAAGTCT 
TTTCCAGATC CTGCAAACTG CAACTTAAAT AGTCATCCTA GTATACACAA TGAAAGTGAT 
CAACCAAAAT CCCTAGAAAT TATAACAAAT AGAGAACTAA AAGAAGGATC AACACTTCAA 
CCAAGTCTTC TATCCCTAAT GAAAGATAGG AGATTAACGC TAGAACAAGT AGTGGCCATA 
GAAGCTCTAA CACAACTTTC AGAAGCCCCA TCAGAAAATT CCTCACCGTC GAAGTCAGAG 
AAGGATGAAG AAACACAACA GAGAACAGCT AGTTTGCTTA ACAGCTGTAA AGCTATCCTG 
TATTCTGTAA GAAAAGACCT CCAAGACCCA AATTTACAAG GGGGGCCACA AAGCCTTCAT 
CACTGTCCAT CTCTGGAAAA GCAAAGTTCA TGCAACACGT TGATGTTCAA TGGCCAAAAT 
ATTTCTAAGT CACACAACAG CTCATCTAGT GACCAAGCAT CCACAAAGGC ACAGGAATAT 
TCAATAGTCA CAAATTCAGT ATCTGTTTTT ATACCAAATT CAAATTCATC TAAAACCGAT 
ACCAATAAAA ATGTTGCTCA AGGTAGAACT GCTCTTGACA GTTCCAAAAA TTTGCATCAG 
TTGACAACAA CAACTAGTAA GTCAGAGTAT TGTAACCAGT CACTGGACAG CAGCATCAAG 
TTGGACTCAA AGGATGATCC CTCATGTCAA GATACAGTTC ATAGTAAAAT AGAAGAAGAT 
GTTGCTGCAC AGTTAACACA ACTTGCATCA ATAATTAAAT TCAATTGTAT AAAACCGGAG 
GACAAAAAAG TTGAAAGTAC ACCAACAAGC CTTGTTGCAT ATAATGTACA GCAAAACTAT 
AGCCAGGAGA AGGGCACAAT AGAGCATAAA CCACCTTCCA GTGTACAAAA TAATCAAGGA 
CCTTCAGTAA CAAAGCAAAA GAACACAATC CAGAAAAAGA CAAAATCTAC CCCATCCAGA 
GATCGACGGA AAAAGAAGCC TGTGATCATA AGTTGTCAAG AAAATGACCA GAAAAAACAG 
GAACAGTTGG CATATGAATA TAGTAAGTTA CATGACATTT GGATAGCATC TAAATTTCAA 
AGATTTGGTC AGTTTGGTCC ACATGACTTT CCTATTCTAT TGGGAAAAAT TCCTCCCCTA 
ACCAAAGTAT GGAAACCACT GACTCAAAGG AGCTCAGCAT TAGAACACAA AAAATTATTT 
CCTCCACTCA CTCAAATAAA ATTTGAGCGG TATTATCCTG AATTAGCCCA GGAGAAGATG 
AAGGTTGAAC CCTTGGATTC TCTCCCCATA ATCCAGTTAA AAGCAGAATC CAACGGACAG 
GCGTTTACAG AAAATGGATA TGCTCAGGTA CAGCCAACAG TGAATGTCAA TCAGAAAGCT 
CATCCTTTAC TCCGGCCAAC CTCTCCAACT AACCAGTGTG CTAATGGGAT GGCTGGCAAT 
GACCAAACAC AGTTCCAACA GGATGTTAAA GACCAAGTCA CACATCAGAG GCTGCCGACA 
TTGCCTGGTA TCTCTCATGA GACCCCTTTA CCGGACCCAG CACAAATTCT CAGGAACCTA 
AATGTAGTAT GTTCAGGTGG AATTACAGTG GTTTCTACCA AAAGTGAAGA GGACGTCTGT 
TCATCTAGTG TTGGAGCATC AGAATTTTCC CCAATAGACA GTGCGCAGAA AAGTTTTAAT 
GATTATGCGA TGAACTTCTT TACTAATCCT ACGAAAACCC TGGTGTCCAC AACAAAAGAT 
TCTGAATTGC CTACCTGCAA CTGTCTTGAT CGAGTTATAC AAAAAGACAA AGGCCCATAT 
TATACACACC TTGGGGCAGG ACCAAGTGTT GCTGCTGTCA GGGAAATCAT GGAGAATAGG 
TATGGTCAAA AAGGAAACGC AATAAGGATA GAAATAGTAG TGTACACGGG GAAAGAAGGA 
AAAAGCTCTA ATGGGTGTCC AGTTGCTAAG TGGGTTTTAA GAAGAAGCAG CGATGAAGAG 
AAAGTTCTTT GTCTCGTTCG ACAGCGTGCA GGCCATCACT GTCCAACAGC TGTGATGGTG 
GTGCTCATCA TGGTATGGGA CGGCATCCCT CTTCCAATGG CTGACAAATT GTACTCGCAG 
CTTACGGAAA GTCTAAAGTC ATACAATGGT CATCCCACAG ATCGAAGATG CACTCTCAAT 
GAAAATCGTA CCTGTACATG TCAAGGAATT GATCCAGAGA CTTGCGGAGC TTCCTTCTCT 
TTTGGCTGTT CTTGGAGTAT GTATTTCAAT GGCTGCAAAT TTGGTAGAAG CCCAAGTCCT 
AGAAGATTTA GAATTGATCC AAGTTCTCCC TTACACCACT ACTATGAAAG AATTACTAAA 
GGACGTAATC CAGAAAGAAG ATATATGAAA CCGGAACCAA TCTGTCCGGA ACACGAGGCC 
ATGGAAAAAA ACCTTGAAGA TAATTTACAG AGTTTGGCTA CAGAATTAGC TCCAATTTAT 
AAGCAGTATG CTCCAGTTGC TTACCAAAAC CAGGTGGCGC TTGAACACGT TGCTCAAGAA 
TGTCGGCTTG GCAAGAAAGA AGGTCGTCCT TTCTCTGGGG TCACTGCTTG CCTAGACTTC 
TGTGCCCACC CCCACAGGGA CATTCACAAC ATGAATAATG GGAGCACGGT GGTCTGCACT 
TTAACACGAG AAGATAACCG CTCATTTGGT GTTATTCCTC AAGATGAGCA GCTGCATGTA 
CTTCCTCTTT ATAAACTTTC AGACACAGAT GAGTTTGGCT CAAGGGACGG AATGGAAGCC 
AAGATCAAAT CTGGGGCCAT CATGGTCCTC GCACCCCACC GCAGAAAAAG AACACGATTT 
ACTCAGCCAG TTCCTCGTTC TGGGAAGAAG AGAGCAGCAA TGATGACAGA GGTTCTCGCA 
CATAAGATAA GGGCTGTGGA AAAGAAATTT ATTCCTCGAA TCAAGCGGAA GAATAATTCA 
ATGACAAACA ACAGTAAGGC TTCATCGCTG CCACTTTTAG GGAATAAAAC AGAGACTGTG 
CAACCTGAAA TAAAAAGTGA AACTGAACCC CACCTTATCT TCAAAGGTTC GGACAACACT 
AAAACCTACT CACCGACCCC ATCCATTCCT CACCCAGTGA AAGAGGCACA CTTAGCTCCA 
GGCTTCTCCT GGTCCCCTAA GACTGCTTCG GTCCCAACAG TTCCTTTTAA GAATGATGCA 
TCAGTTTCGT ACGGGTTTTC AGAAAGAGGT AGCAATCCCC ACTGCACAAT GCCTTCTGCA 
AGACACAGTG GTGCTAATGT AGCTGCTGGG GAGTGTGCTG AAACTGCACA GCCTGGTGAA 
ATGGTTCCTC TTTCCACCTT GTCTACTCCC ATGACAGATT CCCTGGCTTA TTCTGAGCCT 
CCCACTGGTC CATCTGAGCA GCTAACTTCT GATCAGCCAA ACCAGCAGCC CCCATTCATC 
CCTTCTCCTC ATGAACTTGC TTCTTCTCTG GCGGAAGAAG ACGAGCAGCA TTCTGAAGCA 
GATGAGCCTC CATCAGATGA CCCCCTATCA GACGACCCCC TGTCACCTGC TGAGGAGAAA 
CTGCCCCACA TTGTTGAGTA TTGGTCAGAC AGTGAGCACA TCTTCCTGGA TGCCAATGTT 
GGTGGGGTGG CCATAGCGCC CGCCCACGGC TCTGTTCTGA TCGAGTGCGC CAGGCGAGAG 
CTTCACGCCA CAACTCCTGT CGAACACCCC AACCGGAATC ACCCTACACG CCTGTCTCTT 
GTCTTCTACC AGCACAAAAA CCTGAATAAG CCCCAACACG GTTTCGAACT AAACAAGATT 
AAGTTCGAGG CGAAAGAAGC AAAGAATAAG AAAACTAAAG CCTCCGAGCA GAAAGACCAG 
GCAGCTAACG AGGGTCCTGA ACTGTCCACT GAAGTTAATG AATTTAACCA GATTCCTTCC 
CACAAAGCGT TAACATTAAC CCATGACAAT GTTGTCACCG TGTCCCCTTA TGCTCTCACA 
CATGTTGCGG GGCCCTATAA CCATTGGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007121117.1
CDS	1..6453
Translation	MSRSRHARPSKLVRREDLNKKKNSQLKKGSKPANKNVATVKTVSPGKLKQLIQERGVKKKTEPKLPMPVRSLLTRAGAARMNLDRTEVLFQNPESLTCNGFTMALRSTSLSRRLSQPPAVIAKPKKVPPPKNLGKQHECDYKVLTDVGVKHSESDSVPVQDPSIPPDIENLIGIQNASLIKDESQETTQSWSQSVENSKINRSIHSGPAAEVLSGSLEGTRGGKGLFSRETLNDISDSPRMFAQDTLCVPLLQKAALRVTSEGNPSIQLEDLGSRVESLKLSDSYLDPIKSEHDSCPTSSFNKVVPELDLRNCLSIGGSIYPTSLIKLLLAGSEQVTLGAKPDHQEVLKSTPDEQEVPDTTPVLGQAFSAVPHQWEVPGANPVHGEPLGETPGPPEIPGAIPVQGEVFGALLNQQVLGMVGGTASDPDIFPPAPLNPIATCNAFPEWPEPQSTVSSGLEVQGTMQILPSGLGHTPQPSSNSEISSVPPAMAINNIENKKKVHISFLPANTQGFTIAPERGLHHASQGIVHLSQASPSKLEGGSSQISATSMVDINTTVVSRPVSLVSTSSSPSYTTLLPTLEKKKRKRCGVCEPCQQKTNCGECTYCKNRKNSHQICKKRKCEELKKKPSIIVPLEADFDNKPVNGPKSESMEYSSCGHEEEKRLELNSCPLENVTKNEESMTGIEVEKWTQNKKSHLTDHVKGEFNTIKTEAENLKSSEDDRKKILLTDLLEPQKLFAQTVRNGIKNVHYLPTETNLSFKKINIEELGKAFENNPYKFLKDTANHNNAMSAIATSASCDHLKGRSNVLVIQKPGFNCKSFPDPANCNLNSHPSIHNESDQPKSLEIITNRELKEGSTLQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSENSSPSKSEKDEETQQRTASLLNSCKAILYSVRKDLQDPNLQGGPQSLHHCPSLEKQSSCNTLMFNGQNISKSHNSSSSDQASTKAQEYSIVTNSVSVFIPNSNSSKTDTNKNVAQGRTALDSSKNLHQLTTTTSKSEYCNQSLDSSIKLDSKDDPSCQDTVHSKIEEDVAAQLTQLASIIKFNCIKPEDKKVESTPTSLVAYNVQQNYSQEKGTIEHKPPSSVQNNQGPSVTKQKNTIQKKTKSTPSRDRRKKKPVIISCQENDQKKQEQLAYEYSKLHDIWIASKFQRFGQFGPHDFPILLGKIPPLTKVWKPLTQRSSALEHKKLFPPLTQIKFERYYPELAQEKMKVEPLDSLPIIQLKAESNGQAFTENGYAQVQPTVNVNQKAHPLLRPTSPTNQCANGMAGNDQTQFQQDVKDQVTHQRLPTLPGISHETPLPDPAQILRNLNVVCSGGITVVSTKSEEDVCSSSVGASEFSPIDSAQKSFNDYAMNFFTNPTKTLVSTTKDSELPTCNCLDRVIQKDKGPYYTHLGAGPSVAAVREIMENRYGQKGNAIRIEIVVYTGKEGKSSNGCPVAKWVLRRSSDEEKVLCLVRQRAGHHCPTAVMVVLIMVWDGIPLPMADKLYSQLTESLKSYNGHPTDRRCTLNENRTCTCQGIDPETCGASFSFGCSWSMYFNGCKFGRSPSPRRFRIDPSSPLHHYYERITKGRNPERRYMKPEPICPEHEAMEKNLEDNLQSLATELAPIYKQYAPVAYQNQVALEHVAQECRLGKKEGRPFSGVTACLDFCAHPHRDIHNMNNGSTVVCTLTREDNRSFGVIPQDEQLHVLPLYKLSDTDEFGSRDGMEAKIKSGAIMVLAPHRRKRTRFTQPVPRSGKKRAAMMTEVLAHKIRAVEKKFIPRIKRKNNSMTNNSKASSLPLLGNKTETVQPEIKSETEPHLIFKGSDNTKTYSPTPSIPHPVKEAHLAPGFSWSPKTASVPTVPFKNDASVSYGFSERGSNPHCTMPSARHSGANVAAGECAETAQPGEMVPLSTLSTPMTDSLAYSEPPTGPSEQLTSDQPNQQPPFIPSPHELASSLAEEDEQHSEADEPPSDDPLSDDPLSPAEEKLPHIVEYWSDSEHIFLDANVGGVAIAPAHGSVLIECARRELHATTPVEHPNRNHPTRLSLVFYQHKNLNKPQHGFELNKIKFEAKEAKNKKTKASEQKDQAANEGPELSTEVNEFNQIPSHKALTLTHDNVVTVSPYALTHVAGPYNHWV

Target ORF information:

RefSeq Version	XM_007121055.2
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon tet methylcytosine dioxygenase 1 (TET1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007121055.2

ORF Insert Sequence:

ATGTCTCGAT CTCGCCATGC AAGGCCTTCC AAATTAGTCA GGAGGGAAGA TCTAAACAAA 
AAGAAAAACA GCCAACTGAA GAAGGGATCT AAGCCAGCCA ACAAAAATGT GGCGACAGTC 
AAGACTGTGA GCCCTGGAAA ATTAAAGCAA TTAATTCAAG AAAGAGGTGT TAAGAAAAAA 
ACAGAACCCA AACTGCCTAT GCCAGTCAGA AGCCTTCTGA CAAGAGCTGG AGCAGCACGA 
ATGAATTTGG ATAGGACTGA GGTTCTTTTT CAGAACCCAG AGTCCTTAAC TTGCAATGGG 
TTTACAATGG CTCTCCGAAG CACCTCTCTT AGCAGACGAC TCTCCCAACC CCCAGCGGTC 
ATAGCCAAAC CCAAGAAAGT TCCACCTCCT AAGAATCTAG GAAAGCAACA TGAGTGTGAT 
TATAAGGTAC TTACTGACGT GGGAGTAAAG CACTCAGAAA GTGATTCAGT CCCAGTGCAA 
GACCCCTCAA TCCCTCCTGA TATAGAGAAT CTAATTGGCA TACAAAATGC ATCATTAATA 
AAAGATGAGA GCCAAGAGAC AACCCAGTCT TGGTCCCAAA GTGTGGAGAA TTCCAAAATA 
AATCGCTCTA TTCATAGTGG CCCTGCAGCA GAGGTTCTTT CTGGGTCATT GGAAGGGACA 
CGTGGTGGTA AAGGACTATT CTCTAGAGAG ACATTGAATG ATATCAGTGA TTCCCCTAGA 
ATGTTTGCTC AGGACACTCT CTGTGTTCCT TTGCTCCAAA AAGCAGCCCT CAGGGTAACC 
TCTGAAGGAA ACCCCAGCAT TCAGTTAGAA GATTTGGGTT CACGAGTAGA ATCTCTTAAG 
CTGTCTGATT CTTACCTGGA TCCCATCAAA AGTGAACATG ATTCCTGCCC CACCTCCAGC 
TTTAATAAGG TTGTACCTGA ATTGGACCTT AGAAACTGTT TGTCTATTGG TGGGTCAATA 
TATCCTACCT CATTAATAAA ACTCCTCTTG GCAGGCTCAG AACAAGTAAC CCTTGGTGCT 
AAACCAGATC ATCAAGAGGT ACTCAAATCT ACCCCAGATG AACAGGAAGT TCCTGATACC 
ACCCCTGTCC TAGGACAGGC CTTTAGTGCT GTCCCACATC AATGGGAAGT TCCTGGTGCT 
AACCCAGTTC ATGGAGAGCC TCTGGGTGAG ACCCCAGGCC CACCAGAGAT TCCTGGTGCT 
ATTCCAGTCC AAGGAGAGGT CTTTGGTGCT CTCTTAAATC AACAAGTCCT CGGTATGGTT 
GGTGGTACTG CCTCAGACCC CGATATCTTT CCTCCTGCAC CTCTTAACCC AATTGCTACC 
TGTAATGCTT TCCCAGAATG GCCTGAGCCC CAGAGCACTG TTTCATCTGG ACTTGAGGTC 
CAGGGCACTA TGCAGATTTT GCCTTCGGGC TTAGGTCACA CTCCTCAACC ATCATCAAAC 
TCAGAGATAA GTTCGGTACC TCCTGCAATG GCTATAAACA ATATAGAAAA TAAGAAGAAG 
GTTCATATAA GCTTTCTGCC AGCTAACACT CAGGGGTTCA CAATAGCCCC TGAGAGAGGA 
CTCCACCATG CTTCACAGGG CATTGTCCAC CTCTCCCAGG CCAGTCCCAG CAAATTGGAA 
GGAGGGAGCT CCCAGATCAG TGCCACCAGC ATGGTTGACA TCAACACCAC AGTGGTGTCT 
AGGCCAGTGT CACTTGTCAG TACCTCCTCT TCTCCTTCCT ACACAACGTT GCTACCTACT 
TTGGAAAAGA AGAAACGAAA GCGATGTGGG GTCTGTGAGC CCTGCCAGCA GAAGACCAAT 
TGTGGTGAAT GTACCTACTG CAAGAATAGA AAGAACAGCC ATCAGATCTG TAAGAAGAGG 
AAATGTGAGG AACTGAAAAA GAAGCCATCC ATCATTGTGC CTTTGGAGGC AGATTTTGAC 
AACAAACCAG TAAATGGCCC CAAGTCAGAA TCCATGGAAT ACAGTAGTTG TGGTCATGAG 
GAAGAAAAAA GATTGGAATT AAATTCATGT CCCTTAGAAA ATGTAACTAA AAATGAAGAA 
AGTATGACAG GTATTGAAGT GGAAAAGTGG ACACAAAACA AGAAATCACA CTTAACTGAT 
CATGTTAAAG GAGAATTTAA TACAATTAAA ACAGAAGCTG AAAATTTGAA AAGCTCTGAA 
GATGACAGGA AAAAAATCCT ACTTACTGAC CTCCTTGAAC CTCAAAAATT GTTTGCACAA 
ACTGTAAGAA ACGGCATTAA AAATGTACAC TATTTGCCAA CTGAAACAAA TTTGTCTTTT 
AAAAAAATCA ACATTGAAGA ACTTGGCAAG GCATTTGAAA ACAATCCTTA TAAATTCCTG 
AAAGACACTG CAAACCATAA CAATGCCATG AGCGCCATTG CTACTAGTGC GAGCTGTGAT 
CATCTCAAGG GGAGAAGTAA TGTTTTGGTC ATTCAGAAGC CTGGCTTTAA TTGTAAGTCT 
TTTCCAGATC CTGCAAACTG CAACTTAAAT AGTCATCCTA GTATACACAA TGAAAGTGAT 
CAACCAAAAT CCCTAGAAAT TATAACAAAT AGAGAACTAA AAGAAGGATC AACACTTCAA 
CCAAGTCTTC TATCCCTAAT GAAAGATAGG AGATTAACGC TAGAACAAGT AGTGGCCATA 
GAAGCTCTAA CACAACTTTC AGAAGCCCCA TCAGAAAATT CCTCACCGTC GAAGTCAGAG 
AAGGATGAAG AAACACAACA GAGAACAGCT AGTTTGCTTA ACAGCTGTAA AGCTATCCTG 
TATTCTGTAA GAAAAGACCT CCAAGACCCA AATTTACAAG GGGGGCCACA AAGCCTTCAT 
CACTGTCCAT CTCTGGAAAA GCAAAGTTCA TGCAACACGT TGATGTTCAA TGGCCAAAAT 
ATTTCTAAGT CACACAACAG CTCATCTAGT GACCAAGCAT CCACAAAGGC ACAGGAATAT 
TCAATAGTCA CAAATTCAGT ATCTGTTTTT ATACCAAATT CAAATTCATC TAAAACCGAT 
ACCAATAAAA ATGTTGCTCA AGGTAGAACT GCTCTTGACA GTTCCAAAAA TTTGCATCAG 
TTGACAACAA CAACTAGTAA GTCAGAGTAT TGTAACCAGT CACTGGACAG CAGCATCAAG 
TTGGACTCAA AGGATGATCC CTCATGTCAA GATACAGTTC ATAGTAAAAT AGAAGAAGAT 
GTTGCTGCAC AGTTAACACA ACTTGCATCA ATAATTAAAT TCAATTGTAT AAAACCGGAG 
GACAAAAAAG TTGAAAGTAC ACCAACAAGC CTTGTTGCAT ATAATGTACA GCAAAACTAT 
AGCCAGGAGA AGGGCACAAT AGAGCATAAA CCACCTTCCA GTGTACAAAA TAATCAAGGA 
CCTTCAGTAA CAAAGCAAAA GAACACAATC CAGAAAAAGA CAAAATCTAC CCCATCCAGA 
GATCGACGGA AAAAGAAGCC TGTGATCATA AGTTGTCAAG AAAATGACCA GAAAAAACAG 
GAACAGTTGG CATATGAATA TAGTAAGTTA CATGACATTT GGATAGCATC TAAATTTCAA 
AGATTTGGTC AGTTTGGTCC ACATGACTTT CCTATTCTAT TGGGAAAAAT TCCTCCCCTA 
ACCAAAGTAT GGAAACCACT GACTCAAAGG AGCTCAGCAT TAGAACACAA AAAATTATTT 
CCTCCACTCA CTCAAATAAA ATTTGAGCGG TATTATCCTG AATTAGCCCA GGAGAAGATG 
AAGGTTGAAC CCTTGGATTC TCTCCCCATA ATCCAGTTAA AAGCAGAATC CAACGGACAG 
GCGTTTACAG AAAATGGATA TGCTCAGGTA CAGCCAACAG TGAATGTCAA TCAGAAAGCT 
CATCCTTTAC TCCGGCCAAC CTCTCCAACT AACCAGTGTG CTAATGGGAT GGCTGGCAAT 
GACCAAACAC AGTTCCAACA GGATGTTAAA GACCAAGTCA CACATCAGAG GCTGCCGACA 
TTGCCTGGTA TCTCTCATGA GACCCCTTTA CCGGACCCAG CACAAATTCT CAGGAACCTA 
AATGTAGTAT GTTCAGGTGG AATTACAGTG GTTTCTACCA AAAGTGAAGA GGACGTCTGT 
TCATCTAGTG TTGGAGCATC AGAATTTTCC CCAATAGACA GTGCGCAGAA AAGTTTTAAT 
GATTATGCGA TGAACTTCTT TACTAATCCT ACGAAAACCC TGGTGTCCAC AACAAAAGAT 
TCTGAATTGC CTACCTGCAA CTGTCTTGAT CGAGTTATAC AAAAAGACAA AGGCCCATAT 
TATACACACC TTGGGGCAGG ACCAAGTGTT GCTGCTGTCA GGGAAATCAT GGAGAATAGG 
TATGGTCAAA AAGGAAACGC AATAAGGATA GAAATAGTAG TGTACACGGG GAAAGAAGGA 
AAAAGCTCTA ATGGGTGTCC AGTTGCTAAG TGGGTTTTAA GAAGAAGCAG CGATGAAGAG 
AAAGTTCTTT GTCTCGTTCG ACAGCGTGCA GGCCATCACT GTCCAACAGC TGTGATGGTG 
GTGCTCATCA TGGTATGGGA CGGCATCCCT CTTCCAATGG CTGACAAATT GTACTCGCAG 
CTTACGGAAA GTCTAAAGTC ATACAATGGT CATCCCACAG ATCGAAGATG CACTCTCAAT 
GAAAATCGTA CCTGTACATG TCAAGGAATT GATCCAGAGA CTTGCGGAGC TTCCTTCTCT 
TTTGGCTGTT CTTGGAGTAT GTATTTCAAT GGCTGCAAAT TTGGTAGAAG CCCAAGTCCT 
AGAAGATTTA GAATTGATCC AAGTTCTCCC TTACACCACT ACTATGAAAG AATTACTAAA 
GGACGTAATC CAGAAAGAAG ATATATGAAA CCGGAACCAA TCTGTCCGGA ACACGAGGCC 
ATGGAAAAAA ACCTTGAAGA TAATTTACAG AGTTTGGCTA CAGAATTAGC TCCAATTTAT 
AAGCAGTATG CTCCAGTTGC TTACCAAAAC CAGGTGGCGC TTGAACACGT TGCTCAAGAA 
TGTCGGCTTG GCAAGAAAGA AGGTCGTCCT TTCTCTGGGG TCACTGCTTG CCTAGACTTC 
TGTGCCCACC CCCACAGGGA CATTCACAAC ATGAATAATG GGAGCACGGT GGTCTGCACT 
TTAACACGAG AAGATAACCG CTCATTTGGT GTTATTCCTC AAGATGAGCA GCTGCATGTA 
CTTCCTCTTT ATAAACTTTC AGACACAGAT GAGTTTGGCT CAAGGGACGG AATGGAAGCC 
AAGATCAAAT CTGGGGCCAT CATGGTCCTC GCACCCCACC GCAGAAAAAG AACACGATTT 
ACTCAGCCAG TTCCTCGTTC TGGGAAGAAG AGAGCAGCAA TGATGACAGA GGTTCTCGCA 
CATAAGATAA GGGCTGTGGA AAAGAAATTT ATTCCTCGAA TCAAGCGGAA GAATAATTCA 
ATGACAAACA ACAGTAAGGC TTCATCGCTG CCACTTTTAG GGAATAAAAC AGAGACTGTG 
CAACCTGAAA TAAAAAGTGA AACTGAACCC CACCTTATCT TCAAAGGTTC GGACAACACT 
AAAACCTACT CACCGACCCC ATCCATTCCT CACCCAGTGA AAGAGGCACA CTTAGCTCCA 
GGCTTCTCCT GGTCCCCTAA GACTGCTTCG GTCCCAACAG TTCCTTTTAA GAATGATGCA 
TCAGTTTCGT ACGGGTTTTC AGAAAGAGGT AGCAATCCCC ACTGCACAAT GCCTTCTGCA 
AGACACAGTG GTGCTAATGT AGCTGCTGGG GAGTGTGCTG AAACTGCACA GCCTGGTGAA 
ATGGTTCCTC TTTCCACCTT GTCTACTCCC ATGACAGATT CCCTGGCTTA TTCTGAGCCT 
CCCACTGGTC CATCTGAGCA GCTAACTTCT GATCAGCCAA ACCAGCAGCC CCCATTCATC 
CCTTCTCCTC ATGAACTTGC TTCTTCTCTG GCGGAAGAAG ACGAGCAGCA TTCTGAAGCA 
GATGAGCCTC CATCAGATGA CCCCCTATCA GACGACCCCC TGTCACCTGC TGAGGAGAAA 
CTGCCCCACA TTGTTGAGTA TTGGTCAGAC AGTGAGCACA TCTTCCTGGA TGCCAATGTT 
GGTGGGGTGG CCATAGCGCC CGCCCACGGC TCTGTTCTGA TCGAGTGCGC CAGGCGAGAG 
CTTCACGCCA CAACTCCTGT CGAACACCCC AACCGGAATC ACCCTACACG CCTGTCTCTT 
GTCTTCTACC AGCACAAAAA CCTGAATAAG CCCCAACACG GTTTCGAACT AAACAAGATT 
AAGTTCGAGG CGAAAGAAGC AAAGAATAAG AAAACTAAAG CCTCCGAGCA GAAAGACCAG 
GCAGCTAACG AGGGTCCTGA ACTGTCCACT GAAGTTAATG AATTTAACCA GATTCCTTCC 
CACAAAGCGT TAACATTAAC CCATGACAAT GTTGTCACCG TGTCCCCTTA TGCTCTCACA 
CATGTTGCGG GGCCCTATAA CCATTGGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TET1 ( XM_007121056.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_007121118.1 Physeter catodon tet methylcytosine dioxygenase 1 (TET1), transcript variant X3, mRNA.

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CloneID	OPh133401
Clone ID Related Accession (Same CDS sequence)	XM_007121056.1
Accession Version	XM_007121056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	methylcytosine dioxygenase TET1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041233.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGAT CTCGCCATGC AAGGCCTTCC AAATTAGTCA GGAGGGAAGA TCTAAACAAA 
AAGAAAAACA GCCAACTGAA GAAGGGATCT AAGCCAGCCA ACAAAAATGT GGCGACAGTC 
AAGACTGTGA GCCCTGGAAA ATTAAAGCAA TTAATTCAAG AAAGAGGTGT TAAGAAAAAA 
ACAGAACCCA AACTGCCTAT GCCAGTCAGA AGCCTTCTGA CAAGAGCTGG AGCAGCACGA 
ATGAATTTGG ATAGGACTGA GGTTCTTTTT CAGAACCCAG AGTCCTTAAC TTGCAATGGG 
TTTACAATGG CTCTCCGAAG CACCTCTCTT AGCAGACGAC TCTCCCAACC CCCAGCGGTC 
ATAGCCAAAC CCAAGAAAGT TCCACCTCCT AAGAATCTAG GAAAGCAACA TGAGTGTGAT 
TATAAGGTAC TTACTGACGT GGGAGTAAAG CACTCAGAAA GTGATTCAGT CCCAGTGCAA 
GACCCCTCAA TCCCTCCTGA TATAGAGAAT CTAATTGGCA TACAAAATGC ATCATTAATA 
AAAGATGAGA GCCAAGAGAC AACCCAGTCT TGGTCCCAAA GTGTGGAGAA TTCCAAAATA 
AATCGCTCTA TTCATAGTGG CCCTGCAGCA GAGGTTCTTT CTGGGTCATT GGAAGGGACA 
CGTGGTGGTA AAGGACTATT CTCTAGAGAG ACATTGAATG ATATCAGTGA TTCCCCTAGA 
ATGTTTGCTC AGGACACTCT CTGTGTTCCT TTGCTCCAAA AAGCAGCCCT CAGGGTAACC 
TCTGAAGGAA ACCCCAGCAT TCAGTTAGAA GATTTGGGTT CACGAGTAGA ATCTCTTAAG 
CTGTCTGATT CTTACCTGGA TCCCATCAAA AGTGAACATG ATTCCTGCCC CACCTCCAGC 
TTTAATAAGG TTGTACCTGA ATTGGACCTT AGAAACTGTT TGTCTATTGG TGGGTCAATA 
TATCCTACCT CATTAATAAA ACTCCTCTTG GCAGGCTCAG AACAAGTAAC CCTTGGTGCT 
AAACCAGATC ATCAAGAGGT ACTCAAATCT ACCCCAGATG AACAGGAAGT TCCTGATACC 
ACCCCTGTCC TAGGACAGGC CTTTAGTGCT GTCCCACATC AATGGGAAGT TCCTGGTGCT 
AACCCAGTTC ATGGAGAGCC TCTGGGTGAG ACCCCAGGCC CACCAGAGAT TCCTGGTGCT 
ATTCCAGTCC AAGGAGAGGT CTTTGGTGCT CTCTTAAATC AACAAGTCCT CGGTATGGTT 
GGTGGTACTG CCTCAGACCC CGATATCTTT CCTCCTGCAC CTCTTAACCC AATTGCTACC 
TGTAATGCTT TCCCAGAATG GCCTGAGCCC CAGAGCACTG TTTCATCTGG ACTTGAGGTC 
CAGGGCACTA TGCAGATTTT GCCTTCGGGC TTAGGTCACA CTCCTCAACC ATCATCAAAC 
TCAGAGATAA GTTCGGTACC TCCTGCAATG GCTATAAACA ATATAGAAAA TAAGAAGAAG 
GTTCATATAA GCTTTCTGCC AGCTAACACT CAGGGGTTCA CAATAGCCCC TGAGAGAGGA 
CTCCACCATG CTTCACAGGG CATTGTCCAC CTCTCCCAGG CCAGTCCCAG CAAATTGGAA 
GGAGGGAGCT CCCAGATCAG TGCCACCAGC ATGGTTGACA TCAACACCAC AGTGGTGTCT 
AGGCCAGTGT CACTTGTCAG TACCTCCTCT TCTCCTTCCT ACACAACGTT GCTACCTACT 
TTGGAAAAGA AGAAACGAAA GCGATGTGGG GTCTGTGAGC CCTGCCAGCA GAAGACCAAT 
TGTGGTGAAT GTACCTACTG CAAGAATAGA AAGAACAGCC ATCAGATCTG TAAGAAGAGG 
AAATGTGAGG AACTGAAAAA GAAGCCATCC ATCATTGTGC CTTTGGAGGT TATAAAGGAA 
AACAAGAGGC CCCAGAGGGA AAAGAAGCCC AAAGTTTTAA AGGCAGATTT TGACAACAAA 
CCAGTAAATG GCCCCAAGTC AGAATCCATG GAATACAGTA GTTGTGGTCA TGAGGAAGAA 
AAAAGATTGG AATTAAATTC ATGTCCCTTA GAAAATGTAA CTAAAAATGA AGAAAGTATG 
ACAGGTATTG AAGTGGAAAA GTGGACACAA AACAAGAAAT CACACTTAAC TGATCATGTT 
AAAGGAGAAT TTAATACAAT TAAAACAGAA GCTGAAAATT TGAAAAGCTC TGAAGATGAC 
AGGAAAAAAA TCCTACTTAC TGACCTCCTT GAACCTCAAA AATTGTTTGC ACAAACTGTA 
AGAAACGGCA TTAAAAATGT ACACTATTTG CCAACTGAAA CAAATTTGTC TTTTAAAAAA 
ATCAACATTG AAGAACTTGG CAAGGCATTT GAAAACAATC CTTATAAATT CCTGAAAGAC 
ACTGCAAACC ATAACAATGC CATGAGCGCC ATTGCTACTA GTGCGAGCTG TGATCATCTC 
AAGGGGAGAA GTAATGTTTT GGTCATTCAG AAGCCTGGCT TTAATTGTAA GTCTTTTCCA 
GATCCTGCAA ACTGCAACTT AAATAGTCAT CCTAGTATAC ACAATGAAAG TGATCAACCA 
AAATCCCTAG AAATTATAAC AAATAGAGAA CTAAAAGAAG GATCAACACT TCAACCAAGT 
CTTCTATCCC TAATGAAAGA TAGGAGATTA ACGCTAGAAC AAGTAGTGGC CATAGAAGCT 
CTAACACAAC TTTCAGAAGC CCCATCAGAA AATTCCTCAC CGTCGAAGTC AGAGAAGGAT 
GAAGAAACAC AACAGAGAAC AGCTAGTTTG CTTAACAGCT GTAAAGCTAT CCTGTATTCT 
GTAAGAAAAG ACCTCCAAGA CCCAAATTTA CAAGGGGGGC CACAAAGCCT TCATCACTGT 
CCATCTCTGG AAAAGCAAAG TTCATGCAAC ACGTTGATGT TCAATGGCCA AAATATTTCT 
AAGTCACACA ACAGCTCATC TAGTGACCAA GCATCCACAA AGGCACAGGA ATATTCAATA 
GTCACAAATT CAGTATCTGT TTTTATACCA AATTCAAATT CATCTAAAAC CGATACCAAT 
AAAAATGTTG CTCAAGGTAG AACTGCTCTT GACAGTTCCA AAAATTTGCA TCAGTTGACA 
ACAACAACTA GTAAGTCAGA GTATTGTAAC CAGTCACTGG ACAGCAGCAT CAAGTTGGAC 
TCAAAGGATG ATCCCTCATG TCAAGATACA GTTCATAGTA AAATAGAAGA AGATGTTGCT 
GCACAGTTAA CACAACTTGC ATCAATAATT AAATTCAATT GTATAAAACC GGAGGACAAA 
AAAGTTGAAA GTACACCAAC AAGCCTTGTT GCATATAATG TACAGCAAAA CTATAGCCAG 
GAGAAGGGCA CAATAGAGCA TAAACCACCT TCCAGTGTAC AAAATAATCA AGGACCTTCA 
GTAACAAAGC AAAAGAACAC AATCCAGAAA AAGACAAAAT CTACCCCATC CAGAGATCGA 
CGGAAAAAGA AGCCTGTGAT CATAAGTTGT CAAGAAAATG ACCAGAAAAA ACAGGAACAG 
TTGGCATATG AATATAGTAA GTTACATGAC ATTTGGATAG CATCTAAATT TCAAAGATTT 
GGTCAGTTTG GTCCACATGA CTTTCCTATT CTATTGGGAA AAATTCCTCC CCTAACCAAA 
GTATGGAAAC CACTGACTCA AAGGAGCTCA GCATTAGAAC ACAAAAAATT ATTTCCTCCA 
CTCACTCAAA TAAAATTTGA GCGGTATTAT CCTGAATTAG CCCAGGAGAA GATGAAGGTT 
GAACCCTTGG ATTCTCTCCC CATAATCCAG TTAAAAGCAG AATCCAACGG ACAGGCGTTT 
ACAGAAAATG GATATGCTCA GGTACAGCCA ACAGTGAATG TCAATCAGAA AGCTCATCCT 
TTACTCCGGC CAACCTCTCC AACTAACCAG TGTGCTAATG GGATGGCTGG CAATGACCAA 
ACACAGTTCC AACAGGATGT TAAAGACCAA GTCACACATC AGAGGCTGCC GACATTGCCT 
GGTATCTCTC ATGAGACCCC TTTACCGGAC CCAGCACAAA TTCTCAGGAA CCTAAATGTA 
GTATGTTCAG GTGGAATTAC AGTGGTTTCT ACCAAAAGTG AAGAGGACGT CTGTTCATCT 
AGTGTTGGAG CATCAGAATT TTCCCCAATA GACAGTGCGC AGAAAAGTTT TAATGATTAT 
GCGATGAACT TCTTTACTAA TCCTACGAAA ACCCTGGTGT CCACAACAAA AGATTCTGAA 
TTGCCTACCT GCAACTGTCT TGATCGAGTT ATACAAAAAG ACAAAGGCCC ATATTATACA 
CACCTTGGGG CAGGACCAAG TGTTGCTGCT GTCAGGGAAA TCATGGAGAA TAGGTATGGT 
CAAAAAGGAA ACGCAATAAG GATAGAAATA GTAGTGTACA CGGGGAAAGA AGGAAAAAGC 
TCTAATGGGT GTCCAGTTGC TAAGTGGGTT TTAAGAAGAA GCAGCGATGA AGAGAAAGTT 
CTTTGTCTCG TTCGACAGCG TGCAGGCCAT CACTGTCCAA CAGCTGTGAT GGTGGTGCTC 
ATCATGGTAT GGGACGGCAT CCCTCTTCCA ATGGCTGACA AATTGTACTC GCAGCTTACG 
GAAAGTCTAA AGTCATACAA TGGTCATCCC ACAGATCGAA GATGCACTCT CAATGAAAAT 
CGTACCTGTA CATGTCAAGG AATTGATCCA GAGACTTGCG GAGCTTCCTT CTCTTTTGGC 
TGTTCTTGGA GTATGTATTT CAATGGCTGC AAATTTGGTA GAAGCCCAAG TCCTAGAAGA 
TTTAGAATTG ATCCAAGTTC TCCCTTACAC GAAAAAAACC TTGAAGATAA TTTACAGAGT 
TTGGCTACAG AATTAGCTCC AATTTATAAG CAGTATGCTC CAGTTGCTTA CCAAAACCAG 
GTGGCGCTTG AACACGTTGC TCAAGAATGT CGGCTTGGCA AGAAAGAAGG TCGTCCTTTC 
TCTGGGGTCA CTGCTTGCCT AGACTTCTGT GCCCACCCCC ACAGGGACAT TCACAACATG 
AATAATGGGA GCACGGTGGT CTGCACTTTA ACACGAGAAG ATAACCGCTC ATTTGGTGTT 
ATTCCTCAAG ATGAGCAGCT GCATGTACTT CCTCTTTATA AACTTTCAGA CACAGATGAG 
TTTGGCTCAA GGGACGGAAT GGAAGCCAAG ATCAAATCTG GGGCCATCAT GGTCCTCGCA 
CCCCACCGCA GAAAAAGAAC ACGATTTACT CAGCCAGTTC CTCGTTCTGG GAAGAAGAGA 
GCAGCAATGA TGACAGAGGT TCTCGCACAT AAGATAAGGG CTGTGGAAAA GAAATTTATT 
CCTCGAATCA AGCGGAAGAA TAATTCAATG ACAAACAACA GTAAGGCTTC ATCGCTGCCA 
CTTTTAGGGA ATAAAACAGA GACTGTGCAA CCTGAAATAA AAAGTGAAAC TGAACCCCAC 
CTTATCTTCA AAGGTTCGGA CAACACTAAA ACCTACTCAC CGACCCCATC CATTCCTCAC 
CCAGTGAAAG AGGCACACTT AGCTCCAGGC TTCTCCTGGT CCCCTAAGAC TGCTTCGGTC 
CCAACAGTTC CTTTTAAGAA TGATGCATCA GTTTCGTACG GGTTTTCAGA AAGAGGTAGC 
AATCCCCACT GCACAATGCC TTCTGCAAGA CACAGTGGTG CTAATGTAGC TGCTGGGGAG 
TGTGCTGAAA CTGCACAGCC TGGTGAAATG GTTCCTCTTT CCACCTTGTC TACTCCCATG 
ACAGATTCCC TGGCTTATTC TGAGCCTCCC ACTGGTCCAT CTGAGCAGCT AACTTCTGAT 
CAGCCAAACC AGCAGCCCCC ATTCATCCCT TCTCCTCATG AACTTGCTTC TTCTCTGGCG 
GAAGAAGACG AGCAGCATTC TGAAGCAGAT GAGCCTCCAT CAGATGACCC CCTATCAGAC 
GACCCCCTGT CACCTGCTGA GGAGAAACTG CCCCACATTG TTGAGTATTG GTCAGACAGT 
GAGCACATCT TCCTGGATGC CAATGTTGGT GGGGTGGCCA TAGCGCCCGC CCACGGCTCT 
GTTCTGATCG AGTGCGCCAG GCGAGAGCTT CACGCCACAA CTCCTGTCGA ACACCCCAAC 
CGGAATCACC CTACACGCCT GTCTCTTGTC TTCTACCAGC ACAAAAACCT GAATAAGCCC 
CAACACGGTT TCGAACTAAA CAAGATTAAG TTCGAGGCGA AAGAAGCAAA GAATAAGAAA 
ACTAAAGCCT CCGAGCAGAA AGACCAGGCA GCTAACGAGG GTCCTGAACT GTCCACTGAA 
GTTAATGAAT TTAACCAGAT TCCTTCCCAC AAAGCGTTAA CATTAACCCA TGACAATGTT 
GTCACCGTGT CCCCTTATGC TCTCACACAT GTTGCGGGGC CCTATAACCA TTGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007121118.1
CDS	1..6420
Translation	MSRSRHARPSKLVRREDLNKKKNSQLKKGSKPANKNVATVKTVSPGKLKQLIQERGVKKKTEPKLPMPVRSLLTRAGAARMNLDRTEVLFQNPESLTCNGFTMALRSTSLSRRLSQPPAVIAKPKKVPPPKNLGKQHECDYKVLTDVGVKHSESDSVPVQDPSIPPDIENLIGIQNASLIKDESQETTQSWSQSVENSKINRSIHSGPAAEVLSGSLEGTRGGKGLFSRETLNDISDSPRMFAQDTLCVPLLQKAALRVTSEGNPSIQLEDLGSRVESLKLSDSYLDPIKSEHDSCPTSSFNKVVPELDLRNCLSIGGSIYPTSLIKLLLAGSEQVTLGAKPDHQEVLKSTPDEQEVPDTTPVLGQAFSAVPHQWEVPGANPVHGEPLGETPGPPEIPGAIPVQGEVFGALLNQQVLGMVGGTASDPDIFPPAPLNPIATCNAFPEWPEPQSTVSSGLEVQGTMQILPSGLGHTPQPSSNSEISSVPPAMAINNIENKKKVHISFLPANTQGFTIAPERGLHHASQGIVHLSQASPSKLEGGSSQISATSMVDINTTVVSRPVSLVSTSSSPSYTTLLPTLEKKKRKRCGVCEPCQQKTNCGECTYCKNRKNSHQICKKRKCEELKKKPSIIVPLEVIKENKRPQREKKPKVLKADFDNKPVNGPKSESMEYSSCGHEEEKRLELNSCPLENVTKNEESMTGIEVEKWTQNKKSHLTDHVKGEFNTIKTEAENLKSSEDDRKKILLTDLLEPQKLFAQTVRNGIKNVHYLPTETNLSFKKINIEELGKAFENNPYKFLKDTANHNNAMSAIATSASCDHLKGRSNVLVIQKPGFNCKSFPDPANCNLNSHPSIHNESDQPKSLEIITNRELKEGSTLQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSENSSPSKSEKDEETQQRTASLLNSCKAILYSVRKDLQDPNLQGGPQSLHHCPSLEKQSSCNTLMFNGQNISKSHNSSSSDQASTKAQEYSIVTNSVSVFIPNSNSSKTDTNKNVAQGRTALDSSKNLHQLTTTTSKSEYCNQSLDSSIKLDSKDDPSCQDTVHSKIEEDVAAQLTQLASIIKFNCIKPEDKKVESTPTSLVAYNVQQNYSQEKGTIEHKPPSSVQNNQGPSVTKQKNTIQKKTKSTPSRDRRKKKPVIISCQENDQKKQEQLAYEYSKLHDIWIASKFQRFGQFGPHDFPILLGKIPPLTKVWKPLTQRSSALEHKKLFPPLTQIKFERYYPELAQEKMKVEPLDSLPIIQLKAESNGQAFTENGYAQVQPTVNVNQKAHPLLRPTSPTNQCANGMAGNDQTQFQQDVKDQVTHQRLPTLPGISHETPLPDPAQILRNLNVVCSGGITVVSTKSEEDVCSSSVGASEFSPIDSAQKSFNDYAMNFFTNPTKTLVSTTKDSELPTCNCLDRVIQKDKGPYYTHLGAGPSVAAVREIMENRYGQKGNAIRIEIVVYTGKEGKSSNGCPVAKWVLRRSSDEEKVLCLVRQRAGHHCPTAVMVVLIMVWDGIPLPMADKLYSQLTESLKSYNGHPTDRRCTLNENRTCTCQGIDPETCGASFSFGCSWSMYFNGCKFGRSPSPRRFRIDPSSPLHEKNLEDNLQSLATELAPIYKQYAPVAYQNQVALEHVAQECRLGKKEGRPFSGVTACLDFCAHPHRDIHNMNNGSTVVCTLTREDNRSFGVIPQDEQLHVLPLYKLSDTDEFGSRDGMEAKIKSGAIMVLAPHRRKRTRFTQPVPRSGKKRAAMMTEVLAHKIRAVEKKFIPRIKRKNNSMTNNSKASSLPLLGNKTETVQPEIKSETEPHLIFKGSDNTKTYSPTPSIPHPVKEAHLAPGFSWSPKTASVPTVPFKNDASVSYGFSERGSNPHCTMPSARHSGANVAAGECAETAQPGEMVPLSTLSTPMTDSLAYSEPPTGPSEQLTSDQPNQQPPFIPSPHELASSLAEEDEQHSEADEPPSDDPLSDDPLSPAEEKLPHIVEYWSDSEHIFLDANVGGVAIAPAHGSVLIECARRELHATTPVEHPNRNHPTRLSLVFYQHKNLNKPQHGFELNKIKFEAKEAKNKKTKASEQKDQAANEGPELSTEVNEFNQIPSHKALTLTHDNVVTVSPYALTHVAGPYNHWV

Target ORF information:

RefSeq Version	XM_007121056.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon tet methylcytosine dioxygenase 1 (TET1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007121056.1

ORF Insert Sequence:

ATGTCTCGAT CTCGCCATGC AAGGCCTTCC AAATTAGTCA GGAGGGAAGA TCTAAACAAA 
AAGAAAAACA GCCAACTGAA GAAGGGATCT AAGCCAGCCA ACAAAAATGT GGCGACAGTC 
AAGACTGTGA GCCCTGGAAA ATTAAAGCAA TTAATTCAAG AAAGAGGTGT TAAGAAAAAA 
ACAGAACCCA AACTGCCTAT GCCAGTCAGA AGCCTTCTGA CAAGAGCTGG AGCAGCACGA 
ATGAATTTGG ATAGGACTGA GGTTCTTTTT CAGAACCCAG AGTCCTTAAC TTGCAATGGG 
TTTACAATGG CTCTCCGAAG CACCTCTCTT AGCAGACGAC TCTCCCAACC CCCAGCGGTC 
ATAGCCAAAC CCAAGAAAGT TCCACCTCCT AAGAATCTAG GAAAGCAACA TGAGTGTGAT 
TATAAGGTAC TTACTGACGT GGGAGTAAAG CACTCAGAAA GTGATTCAGT CCCAGTGCAA 
GACCCCTCAA TCCCTCCTGA TATAGAGAAT CTAATTGGCA TACAAAATGC ATCATTAATA 
AAAGATGAGA GCCAAGAGAC AACCCAGTCT TGGTCCCAAA GTGTGGAGAA TTCCAAAATA 
AATCGCTCTA TTCATAGTGG CCCTGCAGCA GAGGTTCTTT CTGGGTCATT GGAAGGGACA 
CGTGGTGGTA AAGGACTATT CTCTAGAGAG ACATTGAATG ATATCAGTGA TTCCCCTAGA 
ATGTTTGCTC AGGACACTCT CTGTGTTCCT TTGCTCCAAA AAGCAGCCCT CAGGGTAACC 
TCTGAAGGAA ACCCCAGCAT TCAGTTAGAA GATTTGGGTT CACGAGTAGA ATCTCTTAAG 
CTGTCTGATT CTTACCTGGA TCCCATCAAA AGTGAACATG ATTCCTGCCC CACCTCCAGC 
TTTAATAAGG TTGTACCTGA ATTGGACCTT AGAAACTGTT TGTCTATTGG TGGGTCAATA 
TATCCTACCT CATTAATAAA ACTCCTCTTG GCAGGCTCAG AACAAGTAAC CCTTGGTGCT 
AAACCAGATC ATCAAGAGGT ACTCAAATCT ACCCCAGATG AACAGGAAGT TCCTGATACC 
ACCCCTGTCC TAGGACAGGC CTTTAGTGCT GTCCCACATC AATGGGAAGT TCCTGGTGCT 
AACCCAGTTC ATGGAGAGCC TCTGGGTGAG ACCCCAGGCC CACCAGAGAT TCCTGGTGCT 
ATTCCAGTCC AAGGAGAGGT CTTTGGTGCT CTCTTAAATC AACAAGTCCT CGGTATGGTT 
GGTGGTACTG CCTCAGACCC CGATATCTTT CCTCCTGCAC CTCTTAACCC AATTGCTACC 
TGTAATGCTT TCCCAGAATG GCCTGAGCCC CAGAGCACTG TTTCATCTGG ACTTGAGGTC 
CAGGGCACTA TGCAGATTTT GCCTTCGGGC TTAGGTCACA CTCCTCAACC ATCATCAAAC 
TCAGAGATAA GTTCGGTACC TCCTGCAATG GCTATAAACA ATATAGAAAA TAAGAAGAAG 
GTTCATATAA GCTTTCTGCC AGCTAACACT CAGGGGTTCA CAATAGCCCC TGAGAGAGGA 
CTCCACCATG CTTCACAGGG CATTGTCCAC CTCTCCCAGG CCAGTCCCAG CAAATTGGAA 
GGAGGGAGCT CCCAGATCAG TGCCACCAGC ATGGTTGACA TCAACACCAC AGTGGTGTCT 
AGGCCAGTGT CACTTGTCAG TACCTCCTCT TCTCCTTCCT ACACAACGTT GCTACCTACT 
TTGGAAAAGA AGAAACGAAA GCGATGTGGG GTCTGTGAGC CCTGCCAGCA GAAGACCAAT 
TGTGGTGAAT GTACCTACTG CAAGAATAGA AAGAACAGCC ATCAGATCTG TAAGAAGAGG 
AAATGTGAGG AACTGAAAAA GAAGCCATCC ATCATTGTGC CTTTGGAGGT TATAAAGGAA 
AACAAGAGGC CCCAGAGGGA AAAGAAGCCC AAAGTTTTAA AGGCAGATTT TGACAACAAA 
CCAGTAAATG GCCCCAAGTC AGAATCCATG GAATACAGTA GTTGTGGTCA TGAGGAAGAA 
AAAAGATTGG AATTAAATTC ATGTCCCTTA GAAAATGTAA CTAAAAATGA AGAAAGTATG 
ACAGGTATTG AAGTGGAAAA GTGGACACAA AACAAGAAAT CACACTTAAC TGATCATGTT 
AAAGGAGAAT TTAATACAAT TAAAACAGAA GCTGAAAATT TGAAAAGCTC TGAAGATGAC 
AGGAAAAAAA TCCTACTTAC TGACCTCCTT GAACCTCAAA AATTGTTTGC ACAAACTGTA 
AGAAACGGCA TTAAAAATGT ACACTATTTG CCAACTGAAA CAAATTTGTC TTTTAAAAAA 
ATCAACATTG AAGAACTTGG CAAGGCATTT GAAAACAATC CTTATAAATT CCTGAAAGAC 
ACTGCAAACC ATAACAATGC CATGAGCGCC ATTGCTACTA GTGCGAGCTG TGATCATCTC 
AAGGGGAGAA GTAATGTTTT GGTCATTCAG AAGCCTGGCT TTAATTGTAA GTCTTTTCCA 
GATCCTGCAA ACTGCAACTT AAATAGTCAT CCTAGTATAC ACAATGAAAG TGATCAACCA 
AAATCCCTAG AAATTATAAC AAATAGAGAA CTAAAAGAAG GATCAACACT TCAACCAAGT 
CTTCTATCCC TAATGAAAGA TAGGAGATTA ACGCTAGAAC AAGTAGTGGC CATAGAAGCT 
CTAACACAAC TTTCAGAAGC CCCATCAGAA AATTCCTCAC CGTCGAAGTC AGAGAAGGAT 
GAAGAAACAC AACAGAGAAC AGCTAGTTTG CTTAACAGCT GTAAAGCTAT CCTGTATTCT 
GTAAGAAAAG ACCTCCAAGA CCCAAATTTA CAAGGGGGGC CACAAAGCCT TCATCACTGT 
CCATCTCTGG AAAAGCAAAG TTCATGCAAC ACGTTGATGT TCAATGGCCA AAATATTTCT 
AAGTCACACA ACAGCTCATC TAGTGACCAA GCATCCACAA AGGCACAGGA ATATTCAATA 
GTCACAAATT CAGTATCTGT TTTTATACCA AATTCAAATT CATCTAAAAC CGATACCAAT 
AAAAATGTTG CTCAAGGTAG AACTGCTCTT GACAGTTCCA AAAATTTGCA TCAGTTGACA 
ACAACAACTA GTAAGTCAGA GTATTGTAAC CAGTCACTGG ACAGCAGCAT CAAGTTGGAC 
TCAAAGGATG ATCCCTCATG TCAAGATACA GTTCATAGTA AAATAGAAGA AGATGTTGCT 
GCACAGTTAA CACAACTTGC ATCAATAATT AAATTCAATT GTATAAAACC GGAGGACAAA 
AAAGTTGAAA GTACACCAAC AAGCCTTGTT GCATATAATG TACAGCAAAA CTATAGCCAG 
GAGAAGGGCA CAATAGAGCA TAAACCACCT TCCAGTGTAC AAAATAATCA AGGACCTTCA 
GTAACAAAGC AAAAGAACAC AATCCAGAAA AAGACAAAAT CTACCCCATC CAGAGATCGA 
CGGAAAAAGA AGCCTGTGAT CATAAGTTGT CAAGAAAATG ACCAGAAAAA ACAGGAACAG 
TTGGCATATG AATATAGTAA GTTACATGAC ATTTGGATAG CATCTAAATT TCAAAGATTT 
GGTCAGTTTG GTCCACATGA CTTTCCTATT CTATTGGGAA AAATTCCTCC CCTAACCAAA 
GTATGGAAAC CACTGACTCA AAGGAGCTCA GCATTAGAAC ACAAAAAATT ATTTCCTCCA 
CTCACTCAAA TAAAATTTGA GCGGTATTAT CCTGAATTAG CCCAGGAGAA GATGAAGGTT 
GAACCCTTGG ATTCTCTCCC CATAATCCAG TTAAAAGCAG AATCCAACGG ACAGGCGTTT 
ACAGAAAATG GATATGCTCA GGTACAGCCA ACAGTGAATG TCAATCAGAA AGCTCATCCT 
TTACTCCGGC CAACCTCTCC AACTAACCAG TGTGCTAATG GGATGGCTGG CAATGACCAA 
ACACAGTTCC AACAGGATGT TAAAGACCAA GTCACACATC AGAGGCTGCC GACATTGCCT 
GGTATCTCTC ATGAGACCCC TTTACCGGAC CCAGCACAAA TTCTCAGGAA CCTAAATGTA 
GTATGTTCAG GTGGAATTAC AGTGGTTTCT ACCAAAAGTG AAGAGGACGT CTGTTCATCT 
AGTGTTGGAG CATCAGAATT TTCCCCAATA GACAGTGCGC AGAAAAGTTT TAATGATTAT 
GCGATGAACT TCTTTACTAA TCCTACGAAA ACCCTGGTGT CCACAACAAA AGATTCTGAA 
TTGCCTACCT GCAACTGTCT TGATCGAGTT ATACAAAAAG ACAAAGGCCC ATATTATACA 
CACCTTGGGG CAGGACCAAG TGTTGCTGCT GTCAGGGAAA TCATGGAGAA TAGGTATGGT 
CAAAAAGGAA ACGCAATAAG GATAGAAATA GTAGTGTACA CGGGGAAAGA AGGAAAAAGC 
TCTAATGGGT GTCCAGTTGC TAAGTGGGTT TTAAGAAGAA GCAGCGATGA AGAGAAAGTT 
CTTTGTCTCG TTCGACAGCG TGCAGGCCAT CACTGTCCAA CAGCTGTGAT GGTGGTGCTC 
ATCATGGTAT GGGACGGCAT CCCTCTTCCA ATGGCTGACA AATTGTACTC GCAGCTTACG 
GAAAGTCTAA AGTCATACAA TGGTCATCCC ACAGATCGAA GATGCACTCT CAATGAAAAT 
CGTACCTGTA CATGTCAAGG AATTGATCCA GAGACTTGCG GAGCTTCCTT CTCTTTTGGC 
TGTTCTTGGA GTATGTATTT CAATGGCTGC AAATTTGGTA GAAGCCCAAG TCCTAGAAGA 
TTTAGAATTG ATCCAAGTTC TCCCTTACAC GAAAAAAACC TTGAAGATAA TTTACAGAGT 
TTGGCTACAG AATTAGCTCC AATTTATAAG CAGTATGCTC CAGTTGCTTA CCAAAACCAG 
GTGGCGCTTG AACACGTTGC TCAAGAATGT CGGCTTGGCA AGAAAGAAGG TCGTCCTTTC 
TCTGGGGTCA CTGCTTGCCT AGACTTCTGT GCCCACCCCC ACAGGGACAT TCACAACATG 
AATAATGGGA GCACGGTGGT CTGCACTTTA ACACGAGAAG ATAACCGCTC ATTTGGTGTT 
ATTCCTCAAG ATGAGCAGCT GCATGTACTT CCTCTTTATA AACTTTCAGA CACAGATGAG 
TTTGGCTCAA GGGACGGAAT GGAAGCCAAG ATCAAATCTG GGGCCATCAT GGTCCTCGCA 
CCCCACCGCA GAAAAAGAAC ACGATTTACT CAGCCAGTTC CTCGTTCTGG GAAGAAGAGA 
GCAGCAATGA TGACAGAGGT TCTCGCACAT AAGATAAGGG CTGTGGAAAA GAAATTTATT 
CCTCGAATCA AGCGGAAGAA TAATTCAATG ACAAACAACA GTAAGGCTTC ATCGCTGCCA 
CTTTTAGGGA ATAAAACAGA GACTGTGCAA CCTGAAATAA AAAGTGAAAC TGAACCCCAC 
CTTATCTTCA AAGGTTCGGA CAACACTAAA ACCTACTCAC CGACCCCATC CATTCCTCAC 
CCAGTGAAAG AGGCACACTT AGCTCCAGGC TTCTCCTGGT CCCCTAAGAC TGCTTCGGTC 
CCAACAGTTC CTTTTAAGAA TGATGCATCA GTTTCGTACG GGTTTTCAGA AAGAGGTAGC 
AATCCCCACT GCACAATGCC TTCTGCAAGA CACAGTGGTG CTAATGTAGC TGCTGGGGAG 
TGTGCTGAAA CTGCACAGCC TGGTGAAATG GTTCCTCTTT CCACCTTGTC TACTCCCATG 
ACAGATTCCC TGGCTTATTC TGAGCCTCCC ACTGGTCCAT CTGAGCAGCT AACTTCTGAT 
CAGCCAAACC AGCAGCCCCC ATTCATCCCT TCTCCTCATG AACTTGCTTC TTCTCTGGCG 
GAAGAAGACG AGCAGCATTC TGAAGCAGAT GAGCCTCCAT CAGATGACCC CCTATCAGAC 
GACCCCCTGT CACCTGCTGA GGAGAAACTG CCCCACATTG TTGAGTATTG GTCAGACAGT 
GAGCACATCT TCCTGGATGC CAATGTTGGT GGGGTGGCCA TAGCGCCCGC CCACGGCTCT 
GTTCTGATCG AGTGCGCCAG GCGAGAGCTT CACGCCACAA CTCCTGTCGA ACACCCCAAC 
CGGAATCACC CTACACGCCT GTCTCTTGTC TTCTACCAGC ACAAAAACCT GAATAAGCCC 
CAACACGGTT TCGAACTAAA CAAGATTAAG TTCGAGGCGA AAGAAGCAAA GAATAAGAAA 
ACTAAAGCCT CCGAGCAGAA AGACCAGGCA GCTAACGAGG GTCCTGAACT GTCCACTGAA 
GTTAATGAAT TTAACCAGAT TCCTTCCCAC AAAGCGTTAA CATTAACCCA TGACAATGTT 
GTCACCGTGT CCCCTTATGC TCTCACACAT GTTGCGGGGC CCTATAACCA TTGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TET1 ( XM_024132182.1 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_023987950.1 Physeter catodon tet methylcytosine dioxygenase 1 (TET1), transcript variant X1, mRNA.

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CloneID	OPh301139
Clone ID Related Accession (Same CDS sequence)	XM_024132182.1 , XM_024132182.2
Accession Version	XM_024132182.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1519228800000
Organism	Physeter catodon(sperm whale)
Product	methylcytosine dioxygenase TET1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019873592.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGAT CTCGCCATGC AAGGCCTTCC AAATTAGTCA GGAGGGAAGA TCTAAACAAA 
AAGAAAAACA GCCAACTGAA GAAGGGATCT AAGCCAGCCA ACAAAAATGT GGCGACAGTC 
AAGACTGTGA GCCCTGGAAA ATTAAAGCAA TTAATTCAAG AAAGAGGTGT TAAGAAAAAA 
ACAGAACCCA AACTGCCTAT GCCAGTCAGA AGCCTTCTGA CAAGAGCTGG AGCAGCACGA 
ATGAATTTGG ATAGGACTGA GGTTCTTTTT CAGAACCCAG AGTCCTTAAC TTGCAATGGG 
TTTACAATGG CTCTCCGAAG CACCTCTCTT AGCAGACGAC TCTCCCAACC CCCAGCGGTC 
ATAGCCAAAC CCAAGAAAGT TCCACCTCCT AAGAATCTAG GAAAGCAACA TGAGTGTGAT 
TATAAGGTAC TTACTGACGT GGGAGTAAAG CACTCAGAAA GTGATTCAGT CCCAGTGCAA 
GACCCCTCAA TCCCTCCTGA TATAGAGAAT CTAATTGGCA TACAAAATGC ATCATTAATA 
AAAGATGAGA GCCAAGAGAC AACCCAGTCT TGGTCCCAAA GTGTGGAGAA TTCCAAAATA 
AATCGCTCTA TTCATAGTGG CCCTGCAGCA GAGGTTCTTT CTGGGTCATT GGAAGGGACA 
CGTGGTGGTA AAGGACTATT CTCTAGAGAG ACATTGAATG ATATCAGTGA TTCCCCTAGA 
ATGTTTGCTC AGGACACTCT CTGTGTTCCT TTGCTCCAAA AAGCAGCCCT CAGGGTAACC 
TCTGAAGGAA ACCCCAGCAT TCAGTTAGAA GATTTGGGTT CACGAGTAGA ATCTCTTAAG 
CTGTCTGATT CTTACCTGGA TCCCATCAAA AGTGAACATG ATTCCTGCCC CACCTCCAGC 
TTTAATAAGG TTGTACCTGA ATTGGACCTT AGAAACTGTT TGTCTATTGG TGGGTCAATA 
TATCCTACCT CATTAATAAA ACTCCTCTTG GCAGGCTCAG AACAAGTAAC CCTTGGTGCT 
AAACCAGATC ATCAAGAGGT ACTCAAATCT ACCCCAGATG AACAGGAAGT TCCTGATACC 
ACCCCTGTCC TAGGACAGGC CTTTAGTGCT GTCCCACATC AATGGGAAGT TCCTGGTGCT 
AACCCAGTTC ATGGAGAGCC TCTGGGTGAG ACCCCAGGCC CACCAGAGAT TCCTGGTGCT 
ATTCCAGTCC AAGGAGAGGT CTTTGGTGCT CTCTTAAATC AACAAGTCCT CGGTATGGTT 
GGTGGTACTG CCTCAGACCC CGATATCTTT CCTCCTGCAC CTCTTAACCC AATTGCTACC 
TGTAATGCTT TCCCAGAATG GCCTGAGCCC CAGAGCACTG TTTCATCTGG ACTTGAGGTC 
CAGGGCACTA TGCAGATTTT GCCTTCGGGC TTAGGTCACA CTCCTCAACC ATCATCAAAC 
TCAGAGATAA GTTCGGTACC TCCTGCAATG GCTATAAACA ATATAGAAAA TAAGAAGAAG 
GTTCATATAA GCTTTCTGCC AGCTAACACT CAGGGGTTCA CAATAGCCCC TGAGAGAGGA 
CTCCACCATG CTTCACAGGG CATTGTCCAC CTCTCCCAGG CCAGTCCCAG CAAATTGGAA 
GGAGGGAGCT CCCAGATCAG TGCCACCAGC ATGGTTGACA TCAACACCAC AGTGGTGTCT 
AGGCCAGTGT CACTTGTCAG TACCTCCTCT TCTCCTTCCT ACACAACGTT GCTACCTACT 
TTGGAAAAGA AGAAACGAAA GCGATGTGGG GTCTGTGAGC CCTGCCAGCA GAAGACCAAT 
TGTGGTGAAT GTACCTACTG CAAGAATAGA AAGAACAGCC ATCAGATCTG TAAGAAGAGG 
AAATGTGAGG AACTGAAAAA GAAGCCATCC ATCATTGTGC CTTTGGAGGT TATAAAGGAA 
AACAAGAGGC CCCAGAGGGA AAAGAAGCCC AAAGTTTTAA AGGCAGATTT TGACAACAAA 
CCAGTAAATG GCCCCAAGTC AGAATCCATG GAATACAGTA GTTGTGGTCA TGAGGAAGAA 
AAAAGATTGG AATTAAATTC ATGTCCCTTA GAAAATGTAA CTAAAAATGA AGAAAGTATG 
ACAGGTATTG AAGTGGAAAA GTGGACACAA AACAAGAAAT CACACTTAAC TGATCATGTT 
AAAGGAGAAT TTAATACAAT TAAAACAGAA GCTGAAAATT TGAAAAGCTC TGAAGATGAC 
AGGAAAAAAA TCCTACTTAC TGACCTCCTT GAACCTCAAA AATTGTTTGC ACAAACTGTA 
AGAAACGGCA TTAAAAATGT ACACTATTTG CCAACTGAAA CAAATTTGTC TTTTAAAAAA 
ATCAACATTG AAGAACTTGG CAAGGCATTT GAAAACAATC CTTATAAATT CCTGAAAGAC 
ACTGCAAACC ATAACAATGC CATGAGCGCC ATTGCTACTA GTGCGAGCTG TGATCATCTC 
AAGGGGAGAA GTAATGTTTT GGTCATTCAG AAGCCTGGCT TTAATTGTAA GTCTTTTCCA 
GATCCTGCAA ACTGCAACTT AAATAGTCAT CCTAGTATAC ACAATGAAAG TGATCAACCA 
AAATCCCTAG AAATTATAAC AAATAGAGAA CTAAAAGAAG GATCAACACT TCAACCAAGT 
CTTCTATCCC TAATGAAAGA TAGGAGATTA ACGCTAGAAC AAGTAGTGGC CATAGAAGCT 
CTAACACAAC TTTCAGAAGC CCCATCAGAA AATTCCTCAC CGTCGAAGTC AGAGAAGGAT 
GAAGAAACAC AACAGAGAAC AGCTAGTTTG CTTAACAGCT GTAAAGCTAT CCTGTATTCT 
GTAAGAAAAG ACCTCCAAGA CCCAAATTTA CAAGGGGGGC CACAAAGCCT TCATCACTGT 
CCATCTCTGG AAAAGCAAAG TTCATGCAAC ACGTTGATGT TCAATGGCCA AAATATTTCT 
AAGTCACACA ACAGCTCATC TAGTGACCAA GCATCCACAA AGGCACAGGA ATATTCAATA 
GTCACAAATT CAGTATCTGT TTTTATACCA AATTCAAATT CATCTAAAAC CGATACCAAT 
AAAAATGTTG CTCAAGGTAG AACTGCTCTT GACAGTTCCA AAAATTTGCA TCAGTTGACA 
ACAACAACTA GTAAGTCAGA GTATTGTAAC CAGTCACTGG ACAGCAGCAT CAAGTTGGAC 
TCAAAGGATG ATCCCTCATG TCAAGATACA GTTCATAGTA AAATAGAAGA AGATGTTGCT 
GCACAGTTAA CACAACTTGC ATCAATAATT AAATTCAATT GTATAAAACC GGAGGACAAA 
AAAGTTGAAA GTACACCAAC AAGCCTTGTT GCATATAATG TACAGCAAAA CTATAGCCAG 
GAGAAGGGCA CAATAGAGCA TAAACCACCT TCCAGTGTAC AAAATAATCA AGGACCTTCA 
GTAACAAAGC AAAAGAACAC AATCCAGAAA AAGACAAAAT CTACCCCATC CAGAGATCGA 
CGGAAAAAGA AGCCTGTGAT CATAAGTTGT CAAGAAAATG ACCAGAAAAA ACAGGAACAG 
TTGGCATATG AATATAGTAA GTTACATGAC ATTTGGATAG CATCTAAATT TCAAAGATTT 
GGTCAGTTTG GTCCACATGA CTTTCCTATT CTATTGGGAA AAATTCCTCC CCTAACCAAA 
GTATGGAAAC CACTGACTCA AAGGAGCTCA GCATTAGAAC ACAAAAAATT ATTTCCTCCA 
CTCACTCAAA TAAAATTTGA GCGGTATTAT CCTGAATTAG CCCAGGAGAA GATGAAGGTT 
GAACCCTTGG ATTCTCTCCC CATAATCCAG TTAAAAGCAG AATCCAACGG ACAGGCGTTT 
ACAGAAAATG GATATGCTCA GGTACAGCCA ACAGTGAATG TCAATCAGAA AGCTCATCCT 
TTACTCCGGC CAACCTCTCC AACTAACCAG TGTGCTAATG GGATGGCTGG CAATGACCAA 
ACACAGTTCC AACAGGATGT TAAAGACCAA GTCACACATC AGAGGCTGCC GACATTGCCT 
GGTATCTCTC ATGAGACCCC TTTACCGGAC CCAGCACAAA TTCTCAGGAA CCTAAATGTA 
GTATGTTCAG GTGGAATTAC AGTGGTTTCT ACCAAAAGTG AAGAGGACGT CTGTTCATCT 
AGTGTTGGAG CATCAGAATT TTCCCCAATA GACAGTGCGC AGAAAAGTTT TAATGATTAT 
GCGATGAACT TCTTTACTAA TCCTACGAAA ACCCTGGTGT CCACAACAAA AGATTCTGAA 
TTGCCTACCT GCAACTGTCT TGATCGAGTT ATACAAAAAG ACAAAGGCCC ATATTATACA 
CACCTTGGGG CAGGACCAAG TGTTGCTGCT GTCAGGGAAA TCATGGAGAA TAGGTATGGT 
CAAAAAGGAA ACGCAATAAG GATAGAAATA GTAGTGTACA CGGGGAAAGA AGGAAAAAGC 
TCTAATGGGT GTCCAGTTGC TAAGTGGGTT TTAAGAAGAA GCAGCGATGA AGAGAAAGTT 
CTTTGTCTCG TTCGACAGCG TGCAGGCCAT CACTGTCCAA CAGCTGTGAT GGTGGTGCTC 
ATCATGGTAT GGGACGGCAT CCCTCTTCCA ATGGCTGACA AATTGTACTC GCAGCTTACG 
GAAAGTCTAA AGTCATACAA TGGTCATCCC ACAGATCGAA GATGCACTCT CAATGAAAAT 
CGTACCTGTA CATGTCAAGG AATTGATCCA GAGACTTGCG GAGCTTCCTT CTCTTTTGGC 
TGTTCTTGGA GTATGTATTT CAATGGCTGC AAATTTGGTA GAAGCCCAAG TCCTAGAAGA 
TTTAGAATTG ATCCAAGTTC TCCCTTACAC CACTACTATG AAAGAATTAC TAAAGGACGT 
AATCCAGAAA GAAGATATAT GAAACCGGAA CCAATCTGTC CGGAACACGA GGCCATGGAA 
AAAAACCTTG AAGATAATTT ACAGAGTTTG GCTACAGAAT TAGCTCCAAT TTATAAGCAG 
TATGCTCCAG TTGCTTACCA AAACCAGGTG GCGCTTGAAC ACGTTGCTCA AGAATGTCGG 
CTTGGCAAGA AAGAAGGTCG TCCTTTCTCT GGGGTCACTG CTTGCCTAGA CTTCTGTGCC 
CACCCCCACA GGGACATTCA CAACATGAAT AATGGGAGCA CGGTGGTCTG CACTTTAACA 
CGAGAAGATA ACCGCTCATT TGGTGTTATT CCTCAAGATG AGCAGCTGCA TGTACTTCCT 
CTTTATAAAC TTTCAGACAC AGATGAGTTT GGCTCAAGGG ACGGAATGGA AGCCAAGATC 
AAATCTGGGG CCATCATGGT CCTCGCACCC CACCGCAGAA AAAGAACACG ATTTACTCAG 
CCAGTTCCTC GTTCTGGGAA GAAGAGAGCA GCAATGATGA CAGAGGTTCT CGCACATAAG 
ATAAGGGCTG TGGAAAAGAA ATTTATTCCT CGAATCAAGC GGAAGAATAA TTCAATGACA 
AACAACAGTA AGGCTTCATC GCTGCCACTT TTAGGGAATA AAACAGAGAC TGTGCAACCT 
GAAATAAAAA GTGAAACTGA ACCCCACCTT ATCTTCAAAG GTTCGGACAA CACTAAAACC 
TACTCACCGA CCCCATCCAT TCCTCACCCA GTGAAAGAGG CACACTTAGC TCCAGGCTTC 
TCCTGGTCCC CTAAGACTGC TTCGGTCCCA ACAGTTCCTT TTAAGAATGA TGCATCAGTT 
TCGTACGGGT TTTCAGAAAG AGGTAGCAAT CCCCACTGCA CAATGCCTTC TGCAAGACAC 
AGTGGTGCTA ATGTAGCTGC TGGGGAGTGT GCTGAAACTG CACAGCCTGG TGAAATGGTT 
CCTCTTTCCA CCTTGTCTAC TCCCATGACA GATTCCCTGG CTTATTCTGA GCCTCCCACT 
GGTCCATCTG AGCAGCTAAC TTCTGATCAG CCAAACCAGC AGCCCCCATT CATCCCTTCT 
CCTCATGAAC TTGCTTCTTC TCTGGCGGAA GAAGACGAGC AGCATTCTGA AGCAGATGAG 
CCTCCATCAG ATGACCCCCT ATCAGACGAC CCCCTGTCAC CTGCTGAGGA GAAACTGCCC 
CACATTGTTG AGTATTGGTC AGACAGTGAG CACATCTTCC TGGATGCCAA TGTTGGTGGG 
GTGGCCATAG CGCCCGCCCA CGGCTCTGTT CTGATCGAGT GCGCCAGGCG AGAGCTTCAC 
GCCACAACTC CTGTCGAACA CCCCAACCGG AATCACCCTA CACGCCTGTC TCTTGTCTTC 
TACCAGCACA AAAACCTGAA TAAGCCCCAA CACGGTTTCG AACTAAACAA GATTAAGTTC 
GAGGCGAAAG AAGCAAAGAA TAAGAAAACT AAAGCCTCCG AGCAGAAAGA CCAGGCAGCT 
AACGAGGGTC CTGAACTGTC CACTGAAGTT AATGAATTTA ACCAGATTCC TTCCCACAAA 
GCGTTAACAT TAACCCATGA CAATGTTGTC ACCGTGTCCC CTTATGCTCT CACACATGTT 
GCGGGGCCCT ATAACCATTG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023987950.1
CDS	111..6617
Translation	MSRSRHARPSKLVRREDLNKKKNSQLKKGSKPANKNVATVKTVSPGKLKQLIQERGVKKKTEPKLPMPVRSLLTRAGAARMNLDRTEVLFQNPESLTCNGFTMALRSTSLSRRLSQPPAVIAKPKKVPPPKNLGKQHECDYKVLTDVGVKHSESDSVPVQDPSIPPDIENLIGIQNASLIKDESQETTQSWSQSVENSKINRSIHSGPAAEVLSGSLEGTRGGKGLFSRETLNDISDSPRMFAQDTLCVPLLQKAALRVTSEGNPSIQLEDLGSRVESLKLSDSYLDPIKSEHDSCPTSSFNKVVPELDLRNCLSIGGSIYPTSLIKLLLAGSEQVTLGAKPDHQEVLKSTPDEQEVPDTTPVLGQAFSAVPHQWEVPGANPVHGEPLGETPGPPEIPGAIPVQGEVFGALLNQQVLGMVGGTASDPDIFPPAPLNPIATCNAFPEWPEPQSTVSSGLEVQGTMQILPSGLGHTPQPSSNSEISSVPPAMAINNIENKKKVHISFLPANTQGFTIAPERGLHHASQGIVHLSQASPSKLEGGSSQISATSMVDINTTVVSRPVSLVSTSSSPSYTTLLPTLEKKKRKRCGVCEPCQQKTNCGECTYCKNRKNSHQICKKRKCEELKKKPSIIVPLEVIKENKRPQREKKPKVLKADFDNKPVNGPKSESMEYSSCGHEEEKRLELNSCPLENVTKNEESMTGIEVEKWTQNKKSHLTDHVKGEFNTIKTEAENLKSSEDDRKKILLTDLLEPQKLFAQTVRNGIKNVHYLPTETNLSFKKINIEELGKAFENNPYKFLKDTANHNNAMSAIATSASCDHLKGRSNVLVIQKPGFNCKSFPDPANCNLNSHPSIHNESDQPKSLEIITNRELKEGSTLQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSENSSPSKSEKDEETQQRTASLLNSCKAILYSVRKDLQDPNLQGGPQSLHHCPSLEKQSSCNTLMFNGQNISKSHNSSSSDQASTKAQEYSIVTNSVSVFIPNSNSSKTDTNKNVAQGRTALDSSKNLHQLTTTTSKSEYCNQSLDSSIKLDSKDDPSCQDTVHSKIEEDVAAQLTQLASIIKFNCIKPEDKKVESTPTSLVAYNVQQNYSQEKGTIEHKPPSSVQNNQGPSVTKQKNTIQKKTKSTPSRDRRKKKPVIISCQENDQKKQEQLAYEYSKLHDIWIASKFQRFGQFGPHDFPILLGKIPPLTKVWKPLTQRSSALEHKKLFPPLTQIKFERYYPELAQEKMKVEPLDSLPIIQLKAESNGQAFTENGYAQVQPTVNVNQKAHPLLRPTSPTNQCANGMAGNDQTQFQQDVKDQVTHQRLPTLPGISHETPLPDPAQILRNLNVVCSGGITVVSTKSEEDVCSSSVGASEFSPIDSAQKSFNDYAMNFFTNPTKTLVSTTKDSELPTCNCLDRVIQKDKGPYYTHLGAGPSVAAVREIMENRYGQKGNAIRIEIVVYTGKEGKSSNGCPVAKWVLRRSSDEEKVLCLVRQRAGHHCPTAVMVVLIMVWDGIPLPMADKLYSQLTESLKSYNGHPTDRRCTLNENRTCTCQGIDPETCGASFSFGCSWSMYFNGCKFGRSPSPRRFRIDPSSPLHHYYERITKGRNPERRYMKPEPICPEHEAMEKNLEDNLQSLATELAPIYKQYAPVAYQNQVALEHVAQECRLGKKEGRPFSGVTACLDFCAHPHRDIHNMNNGSTVVCTLTREDNRSFGVIPQDEQLHVLPLYKLSDTDEFGSRDGMEAKIKSGAIMVLAPHRRKRTRFTQPVPRSGKKRAAMMTEVLAHKIRAVEKKFIPRIKRKNNSMTNNSKASSLPLLGNKTETVQPEIKSETEPHLIFKGSDNTKTYSPTPSIPHPVKEAHLAPGFSWSPKTASVPTVPFKNDASVSYGFSERGSNPHCTMPSARHSGANVAAGECAETAQPGEMVPLSTLSTPMTDSLAYSEPPTGPSEQLTSDQPNQQPPFIPSPHELASSLAEEDEQHSEADEPPSDDPLSDDPLSPAEEKLPHIVEYWSDSEHIFLDANVGGVAIAPAHGSVLIECARRELHATTPVEHPNRNHPTRLSLVFYQHKNLNKPQHGFELNKIKFEAKEAKNKKTKASEQKDQAANEGPELSTEVNEFNQIPSHKALTLTHDNVVTVSPYALTHVAGPYNHWV

Target ORF information:

RefSeq Version	XM_024132182.1
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon tet methylcytosine dioxygenase 1 (TET1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024132182.1

ORF Insert Sequence:

ATGTCTCGAT CTCGCCATGC AAGGCCTTCC AAATTAGTCA GGAGGGAAGA TCTAAACAAA 
AAGAAAAACA GCCAACTGAA GAAGGGATCT AAGCCAGCCA ACAAAAATGT GGCGACAGTC 
AAGACTGTGA GCCCTGGAAA ATTAAAGCAA TTAATTCAAG AAAGAGGTGT TAAGAAAAAA 
ACAGAACCCA AACTGCCTAT GCCAGTCAGA AGCCTTCTGA CAAGAGCTGG AGCAGCACGA 
ATGAATTTGG ATAGGACTGA GGTTCTTTTT CAGAACCCAG AGTCCTTAAC TTGCAATGGG 
TTTACAATGG CTCTCCGAAG CACCTCTCTT AGCAGACGAC TCTCCCAACC CCCAGCGGTC 
ATAGCCAAAC CCAAGAAAGT TCCACCTCCT AAGAATCTAG GAAAGCAACA TGAGTGTGAT 
TATAAGGTAC TTACTGACGT GGGAGTAAAG CACTCAGAAA GTGATTCAGT CCCAGTGCAA 
GACCCCTCAA TCCCTCCTGA TATAGAGAAT CTAATTGGCA TACAAAATGC ATCATTAATA 
AAAGATGAGA GCCAAGAGAC AACCCAGTCT TGGTCCCAAA GTGTGGAGAA TTCCAAAATA 
AATCGCTCTA TTCATAGTGG CCCTGCAGCA GAGGTTCTTT CTGGGTCATT GGAAGGGACA 
CGTGGTGGTA AAGGACTATT CTCTAGAGAG ACATTGAATG ATATCAGTGA TTCCCCTAGA 
ATGTTTGCTC AGGACACTCT CTGTGTTCCT TTGCTCCAAA AAGCAGCCCT CAGGGTAACC 
TCTGAAGGAA ACCCCAGCAT TCAGTTAGAA GATTTGGGTT CACGAGTAGA ATCTCTTAAG 
CTGTCTGATT CTTACCTGGA TCCCATCAAA AGTGAACATG ATTCCTGCCC CACCTCCAGC 
TTTAATAAGG TTGTACCTGA ATTGGACCTT AGAAACTGTT TGTCTATTGG TGGGTCAATA 
TATCCTACCT CATTAATAAA ACTCCTCTTG GCAGGCTCAG AACAAGTAAC CCTTGGTGCT 
AAACCAGATC ATCAAGAGGT ACTCAAATCT ACCCCAGATG AACAGGAAGT TCCTGATACC 
ACCCCTGTCC TAGGACAGGC CTTTAGTGCT GTCCCACATC AATGGGAAGT TCCTGGTGCT 
AACCCAGTTC ATGGAGAGCC TCTGGGTGAG ACCCCAGGCC CACCAGAGAT TCCTGGTGCT 
ATTCCAGTCC AAGGAGAGGT CTTTGGTGCT CTCTTAAATC AACAAGTCCT CGGTATGGTT 
GGTGGTACTG CCTCAGACCC CGATATCTTT CCTCCTGCAC CTCTTAACCC AATTGCTACC 
TGTAATGCTT TCCCAGAATG GCCTGAGCCC CAGAGCACTG TTTCATCTGG ACTTGAGGTC 
CAGGGCACTA TGCAGATTTT GCCTTCGGGC TTAGGTCACA CTCCTCAACC ATCATCAAAC 
TCAGAGATAA GTTCGGTACC TCCTGCAATG GCTATAAACA ATATAGAAAA TAAGAAGAAG 
GTTCATATAA GCTTTCTGCC AGCTAACACT CAGGGGTTCA CAATAGCCCC TGAGAGAGGA 
CTCCACCATG CTTCACAGGG CATTGTCCAC CTCTCCCAGG CCAGTCCCAG CAAATTGGAA 
GGAGGGAGCT CCCAGATCAG TGCCACCAGC ATGGTTGACA TCAACACCAC AGTGGTGTCT 
AGGCCAGTGT CACTTGTCAG TACCTCCTCT TCTCCTTCCT ACACAACGTT GCTACCTACT 
TTGGAAAAGA AGAAACGAAA GCGATGTGGG GTCTGTGAGC CCTGCCAGCA GAAGACCAAT 
TGTGGTGAAT GTACCTACTG CAAGAATAGA AAGAACAGCC ATCAGATCTG TAAGAAGAGG 
AAATGTGAGG AACTGAAAAA GAAGCCATCC ATCATTGTGC CTTTGGAGGT TATAAAGGAA 
AACAAGAGGC CCCAGAGGGA AAAGAAGCCC AAAGTTTTAA AGGCAGATTT TGACAACAAA 
CCAGTAAATG GCCCCAAGTC AGAATCCATG GAATACAGTA GTTGTGGTCA TGAGGAAGAA 
AAAAGATTGG AATTAAATTC ATGTCCCTTA GAAAATGTAA CTAAAAATGA AGAAAGTATG 
ACAGGTATTG AAGTGGAAAA GTGGACACAA AACAAGAAAT CACACTTAAC TGATCATGTT 
AAAGGAGAAT TTAATACAAT TAAAACAGAA GCTGAAAATT TGAAAAGCTC TGAAGATGAC 
AGGAAAAAAA TCCTACTTAC TGACCTCCTT GAACCTCAAA AATTGTTTGC ACAAACTGTA 
AGAAACGGCA TTAAAAATGT ACACTATTTG CCAACTGAAA CAAATTTGTC TTTTAAAAAA 
ATCAACATTG AAGAACTTGG CAAGGCATTT GAAAACAATC CTTATAAATT CCTGAAAGAC 
ACTGCAAACC ATAACAATGC CATGAGCGCC ATTGCTACTA GTGCGAGCTG TGATCATCTC 
AAGGGGAGAA GTAATGTTTT GGTCATTCAG AAGCCTGGCT TTAATTGTAA GTCTTTTCCA 
GATCCTGCAA ACTGCAACTT AAATAGTCAT CCTAGTATAC ACAATGAAAG TGATCAACCA 
AAATCCCTAG AAATTATAAC AAATAGAGAA CTAAAAGAAG GATCAACACT TCAACCAAGT 
CTTCTATCCC TAATGAAAGA TAGGAGATTA ACGCTAGAAC AAGTAGTGGC CATAGAAGCT 
CTAACACAAC TTTCAGAAGC CCCATCAGAA AATTCCTCAC CGTCGAAGTC AGAGAAGGAT 
GAAGAAACAC AACAGAGAAC AGCTAGTTTG CTTAACAGCT GTAAAGCTAT CCTGTATTCT 
GTAAGAAAAG ACCTCCAAGA CCCAAATTTA CAAGGGGGGC CACAAAGCCT TCATCACTGT 
CCATCTCTGG AAAAGCAAAG TTCATGCAAC ACGTTGATGT TCAATGGCCA AAATATTTCT 
AAGTCACACA ACAGCTCATC TAGTGACCAA GCATCCACAA AGGCACAGGA ATATTCAATA 
GTCACAAATT CAGTATCTGT TTTTATACCA AATTCAAATT CATCTAAAAC CGATACCAAT 
AAAAATGTTG CTCAAGGTAG AACTGCTCTT GACAGTTCCA AAAATTTGCA TCAGTTGACA 
ACAACAACTA GTAAGTCAGA GTATTGTAAC CAGTCACTGG ACAGCAGCAT CAAGTTGGAC 
TCAAAGGATG ATCCCTCATG TCAAGATACA GTTCATAGTA AAATAGAAGA AGATGTTGCT 
GCACAGTTAA CACAACTTGC ATCAATAATT AAATTCAATT GTATAAAACC GGAGGACAAA 
AAAGTTGAAA GTACACCAAC AAGCCTTGTT GCATATAATG TACAGCAAAA CTATAGCCAG 
GAGAAGGGCA CAATAGAGCA TAAACCACCT TCCAGTGTAC AAAATAATCA AGGACCTTCA 
GTAACAAAGC AAAAGAACAC AATCCAGAAA AAGACAAAAT CTACCCCATC CAGAGATCGA 
CGGAAAAAGA AGCCTGTGAT CATAAGTTGT CAAGAAAATG ACCAGAAAAA ACAGGAACAG 
TTGGCATATG AATATAGTAA GTTACATGAC ATTTGGATAG CATCTAAATT TCAAAGATTT 
GGTCAGTTTG GTCCACATGA CTTTCCTATT CTATTGGGAA AAATTCCTCC CCTAACCAAA 
GTATGGAAAC CACTGACTCA AAGGAGCTCA GCATTAGAAC ACAAAAAATT ATTTCCTCCA 
CTCACTCAAA TAAAATTTGA GCGGTATTAT CCTGAATTAG CCCAGGAGAA GATGAAGGTT 
GAACCCTTGG ATTCTCTCCC CATAATCCAG TTAAAAGCAG AATCCAACGG ACAGGCGTTT 
ACAGAAAATG GATATGCTCA GGTACAGCCA ACAGTGAATG TCAATCAGAA AGCTCATCCT 
TTACTCCGGC CAACCTCTCC AACTAACCAG TGTGCTAATG GGATGGCTGG CAATGACCAA 
ACACAGTTCC AACAGGATGT TAAAGACCAA GTCACACATC AGAGGCTGCC GACATTGCCT 
GGTATCTCTC ATGAGACCCC TTTACCGGAC CCAGCACAAA TTCTCAGGAA CCTAAATGTA 
GTATGTTCAG GTGGAATTAC AGTGGTTTCT ACCAAAAGTG AAGAGGACGT CTGTTCATCT 
AGTGTTGGAG CATCAGAATT TTCCCCAATA GACAGTGCGC AGAAAAGTTT TAATGATTAT 
GCGATGAACT TCTTTACTAA TCCTACGAAA ACCCTGGTGT CCACAACAAA AGATTCTGAA 
TTGCCTACCT GCAACTGTCT TGATCGAGTT ATACAAAAAG ACAAAGGCCC ATATTATACA 
CACCTTGGGG CAGGACCAAG TGTTGCTGCT GTCAGGGAAA TCATGGAGAA TAGGTATGGT 
CAAAAAGGAA ACGCAATAAG GATAGAAATA GTAGTGTACA CGGGGAAAGA AGGAAAAAGC 
TCTAATGGGT GTCCAGTTGC TAAGTGGGTT TTAAGAAGAA GCAGCGATGA AGAGAAAGTT 
CTTTGTCTCG TTCGACAGCG TGCAGGCCAT CACTGTCCAA CAGCTGTGAT GGTGGTGCTC 
ATCATGGTAT GGGACGGCAT CCCTCTTCCA ATGGCTGACA AATTGTACTC GCAGCTTACG 
GAAAGTCTAA AGTCATACAA TGGTCATCCC ACAGATCGAA GATGCACTCT CAATGAAAAT 
CGTACCTGTA CATGTCAAGG AATTGATCCA GAGACTTGCG GAGCTTCCTT CTCTTTTGGC 
TGTTCTTGGA GTATGTATTT CAATGGCTGC AAATTTGGTA GAAGCCCAAG TCCTAGAAGA 
TTTAGAATTG ATCCAAGTTC TCCCTTACAC CACTACTATG AAAGAATTAC TAAAGGACGT 
AATCCAGAAA GAAGATATAT GAAACCGGAA CCAATCTGTC CGGAACACGA GGCCATGGAA 
AAAAACCTTG AAGATAATTT ACAGAGTTTG GCTACAGAAT TAGCTCCAAT TTATAAGCAG 
TATGCTCCAG TTGCTTACCA AAACCAGGTG GCGCTTGAAC ACGTTGCTCA AGAATGTCGG 
CTTGGCAAGA AAGAAGGTCG TCCTTTCTCT GGGGTCACTG CTTGCCTAGA CTTCTGTGCC 
CACCCCCACA GGGACATTCA CAACATGAAT AATGGGAGCA CGGTGGTCTG CACTTTAACA 
CGAGAAGATA ACCGCTCATT TGGTGTTATT CCTCAAGATG AGCAGCTGCA TGTACTTCCT 
CTTTATAAAC TTTCAGACAC AGATGAGTTT GGCTCAAGGG ACGGAATGGA AGCCAAGATC 
AAATCTGGGG CCATCATGGT CCTCGCACCC CACCGCAGAA AAAGAACACG ATTTACTCAG 
CCAGTTCCTC GTTCTGGGAA GAAGAGAGCA GCAATGATGA CAGAGGTTCT CGCACATAAG 
ATAAGGGCTG TGGAAAAGAA ATTTATTCCT CGAATCAAGC GGAAGAATAA TTCAATGACA 
AACAACAGTA AGGCTTCATC GCTGCCACTT TTAGGGAATA AAACAGAGAC TGTGCAACCT 
GAAATAAAAA GTGAAACTGA ACCCCACCTT ATCTTCAAAG GTTCGGACAA CACTAAAACC 
TACTCACCGA CCCCATCCAT TCCTCACCCA GTGAAAGAGG CACACTTAGC TCCAGGCTTC 
TCCTGGTCCC CTAAGACTGC TTCGGTCCCA ACAGTTCCTT TTAAGAATGA TGCATCAGTT 
TCGTACGGGT TTTCAGAAAG AGGTAGCAAT CCCCACTGCA CAATGCCTTC TGCAAGACAC 
AGTGGTGCTA ATGTAGCTGC TGGGGAGTGT GCTGAAACTG CACAGCCTGG TGAAATGGTT 
CCTCTTTCCA CCTTGTCTAC TCCCATGACA GATTCCCTGG CTTATTCTGA GCCTCCCACT 
GGTCCATCTG AGCAGCTAAC TTCTGATCAG CCAAACCAGC AGCCCCCATT CATCCCTTCT 
CCTCATGAAC TTGCTTCTTC TCTGGCGGAA GAAGACGAGC AGCATTCTGA AGCAGATGAG 
CCTCCATCAG ATGACCCCCT ATCAGACGAC CCCCTGTCAC CTGCTGAGGA GAAACTGCCC 
CACATTGTTG AGTATTGGTC AGACAGTGAG CACATCTTCC TGGATGCCAA TGTTGGTGGG 
GTGGCCATAG CGCCCGCCCA CGGCTCTGTT CTGATCGAGT GCGCCAGGCG AGAGCTTCAC 
GCCACAACTC CTGTCGAACA CCCCAACCGG AATCACCCTA CACGCCTGTC TCTTGTCTTC 
TACCAGCACA AAAACCTGAA TAAGCCCCAA CACGGTTTCG AACTAAACAA GATTAAGTTC 
GAGGCGAAAG AAGCAAAGAA TAAGAAAACT AAAGCCTCCG AGCAGAAAGA CCAGGCAGCT 
AACGAGGGTC CTGAACTGTC CACTGAAGTT AATGAATTTA ACCAGATTCC TTCCCACAAA 
GCGTTAACAT TAACCCATGA CAATGTTGTC ACCGTGTCCC CTTATGCTCT CACACATGTT 
GCGGGGCCCT ATAACCATTG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TET1 ( XM_024132182.2 ) cDNA ORF clone, Physeter catodon(sperm whale) -> XP_023987950.1 Physeter catodon tet methylcytosine dioxygenase 1 (TET1), transcript variant X1, mRNA.

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CloneID	OPh301139
Clone ID Related Accession (Same CDS sequence)	XM_024132182.1 , XM_024132182.2
Accession Version	XM_024132182.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Physeter catodon(sperm whale)
Product	methylcytosine dioxygenase TET1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041233.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2019 this sequence version replaced XM_024132182.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physeter catodon Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGAT CTCGCCATGC AAGGCCTTCC AAATTAGTCA GGAGGGAAGA TCTAAACAAA 
AAGAAAAACA GCCAACTGAA GAAGGGATCT AAGCCAGCCA ACAAAAATGT GGCGACAGTC 
AAGACTGTGA GCCCTGGAAA ATTAAAGCAA TTAATTCAAG AAAGAGGTGT TAAGAAAAAA 
ACAGAACCCA AACTGCCTAT GCCAGTCAGA AGCCTTCTGA CAAGAGCTGG AGCAGCACGA 
ATGAATTTGG ATAGGACTGA GGTTCTTTTT CAGAACCCAG AGTCCTTAAC TTGCAATGGG 
TTTACAATGG CTCTCCGAAG CACCTCTCTT AGCAGACGAC TCTCCCAACC CCCAGCGGTC 
ATAGCCAAAC CCAAGAAAGT TCCACCTCCT AAGAATCTAG GAAAGCAACA TGAGTGTGAT 
TATAAGGTAC TTACTGACGT GGGAGTAAAG CACTCAGAAA GTGATTCAGT CCCAGTGCAA 
GACCCCTCAA TCCCTCCTGA TATAGAGAAT CTAATTGGCA TACAAAATGC ATCATTAATA 
AAAGATGAGA GCCAAGAGAC AACCCAGTCT TGGTCCCAAA GTGTGGAGAA TTCCAAAATA 
AATCGCTCTA TTCATAGTGG CCCTGCAGCA GAGGTTCTTT CTGGGTCATT GGAAGGGACA 
CGTGGTGGTA AAGGACTATT CTCTAGAGAG ACATTGAATG ATATCAGTGA TTCCCCTAGA 
ATGTTTGCTC AGGACACTCT CTGTGTTCCT TTGCTCCAAA AAGCAGCCCT CAGGGTAACC 
TCTGAAGGAA ACCCCAGCAT TCAGTTAGAA GATTTGGGTT CACGAGTAGA ATCTCTTAAG 
CTGTCTGATT CTTACCTGGA TCCCATCAAA AGTGAACATG ATTCCTGCCC CACCTCCAGC 
TTTAATAAGG TTGTACCTGA ATTGGACCTT AGAAACTGTT TGTCTATTGG TGGGTCAATA 
TATCCTACCT CATTAATAAA ACTCCTCTTG GCAGGCTCAG AACAAGTAAC CCTTGGTGCT 
AAACCAGATC ATCAAGAGGT ACTCAAATCT ACCCCAGATG AACAGGAAGT TCCTGATACC 
ACCCCTGTCC TAGGACAGGC CTTTAGTGCT GTCCCACATC AATGGGAAGT TCCTGGTGCT 
AACCCAGTTC ATGGAGAGCC TCTGGGTGAG ACCCCAGGCC CACCAGAGAT TCCTGGTGCT 
ATTCCAGTCC AAGGAGAGGT CTTTGGTGCT CTCTTAAATC AACAAGTCCT CGGTATGGTT 
GGTGGTACTG CCTCAGACCC CGATATCTTT CCTCCTGCAC CTCTTAACCC AATTGCTACC 
TGTAATGCTT TCCCAGAATG GCCTGAGCCC CAGAGCACTG TTTCATCTGG ACTTGAGGTC 
CAGGGCACTA TGCAGATTTT GCCTTCGGGC TTAGGTCACA CTCCTCAACC ATCATCAAAC 
TCAGAGATAA GTTCGGTACC TCCTGCAATG GCTATAAACA ATATAGAAAA TAAGAAGAAG 
GTTCATATAA GCTTTCTGCC AGCTAACACT CAGGGGTTCA CAATAGCCCC TGAGAGAGGA 
CTCCACCATG CTTCACAGGG CATTGTCCAC CTCTCCCAGG CCAGTCCCAG CAAATTGGAA 
GGAGGGAGCT CCCAGATCAG TGCCACCAGC ATGGTTGACA TCAACACCAC AGTGGTGTCT 
AGGCCAGTGT CACTTGTCAG TACCTCCTCT TCTCCTTCCT ACACAACGTT GCTACCTACT 
TTGGAAAAGA AGAAACGAAA GCGATGTGGG GTCTGTGAGC CCTGCCAGCA GAAGACCAAT 
TGTGGTGAAT GTACCTACTG CAAGAATAGA AAGAACAGCC ATCAGATCTG TAAGAAGAGG 
AAATGTGAGG AACTGAAAAA GAAGCCATCC ATCATTGTGC CTTTGGAGGT TATAAAGGAA 
AACAAGAGGC CCCAGAGGGA AAAGAAGCCC AAAGTTTTAA AGGCAGATTT TGACAACAAA 
CCAGTAAATG GCCCCAAGTC AGAATCCATG GAATACAGTA GTTGTGGTCA TGAGGAAGAA 
AAAAGATTGG AATTAAATTC ATGTCCCTTA GAAAATGTAA CTAAAAATGA AGAAAGTATG 
ACAGGTATTG AAGTGGAAAA GTGGACACAA AACAAGAAAT CACACTTAAC TGATCATGTT 
AAAGGAGAAT TTAATACAAT TAAAACAGAA GCTGAAAATT TGAAAAGCTC TGAAGATGAC 
AGGAAAAAAA TCCTACTTAC TGACCTCCTT GAACCTCAAA AATTGTTTGC ACAAACTGTA 
AGAAACGGCA TTAAAAATGT ACACTATTTG CCAACTGAAA CAAATTTGTC TTTTAAAAAA 
ATCAACATTG AAGAACTTGG CAAGGCATTT GAAAACAATC CTTATAAATT CCTGAAAGAC 
ACTGCAAACC ATAACAATGC CATGAGCGCC ATTGCTACTA GTGCGAGCTG TGATCATCTC 
AAGGGGAGAA GTAATGTTTT GGTCATTCAG AAGCCTGGCT TTAATTGTAA GTCTTTTCCA 
GATCCTGCAA ACTGCAACTT AAATAGTCAT CCTAGTATAC ACAATGAAAG TGATCAACCA 
AAATCCCTAG AAATTATAAC AAATAGAGAA CTAAAAGAAG GATCAACACT TCAACCAAGT 
CTTCTATCCC TAATGAAAGA TAGGAGATTA ACGCTAGAAC AAGTAGTGGC CATAGAAGCT 
CTAACACAAC TTTCAGAAGC CCCATCAGAA AATTCCTCAC CGTCGAAGTC AGAGAAGGAT 
GAAGAAACAC AACAGAGAAC AGCTAGTTTG CTTAACAGCT GTAAAGCTAT CCTGTATTCT 
GTAAGAAAAG ACCTCCAAGA CCCAAATTTA CAAGGGGGGC CACAAAGCCT TCATCACTGT 
CCATCTCTGG AAAAGCAAAG TTCATGCAAC ACGTTGATGT TCAATGGCCA AAATATTTCT 
AAGTCACACA ACAGCTCATC TAGTGACCAA GCATCCACAA AGGCACAGGA ATATTCAATA 
GTCACAAATT CAGTATCTGT TTTTATACCA AATTCAAATT CATCTAAAAC CGATACCAAT 
AAAAATGTTG CTCAAGGTAG AACTGCTCTT GACAGTTCCA AAAATTTGCA TCAGTTGACA 
ACAACAACTA GTAAGTCAGA GTATTGTAAC CAGTCACTGG ACAGCAGCAT CAAGTTGGAC 
TCAAAGGATG ATCCCTCATG TCAAGATACA GTTCATAGTA AAATAGAAGA AGATGTTGCT 
GCACAGTTAA CACAACTTGC ATCAATAATT AAATTCAATT GTATAAAACC GGAGGACAAA 
AAAGTTGAAA GTACACCAAC AAGCCTTGTT GCATATAATG TACAGCAAAA CTATAGCCAG 
GAGAAGGGCA CAATAGAGCA TAAACCACCT TCCAGTGTAC AAAATAATCA AGGACCTTCA 
GTAACAAAGC AAAAGAACAC AATCCAGAAA AAGACAAAAT CTACCCCATC CAGAGATCGA 
CGGAAAAAGA AGCCTGTGAT CATAAGTTGT CAAGAAAATG ACCAGAAAAA ACAGGAACAG 
TTGGCATATG AATATAGTAA GTTACATGAC ATTTGGATAG CATCTAAATT TCAAAGATTT 
GGTCAGTTTG GTCCACATGA CTTTCCTATT CTATTGGGAA AAATTCCTCC CCTAACCAAA 
GTATGGAAAC CACTGACTCA AAGGAGCTCA GCATTAGAAC ACAAAAAATT ATTTCCTCCA 
CTCACTCAAA TAAAATTTGA GCGGTATTAT CCTGAATTAG CCCAGGAGAA GATGAAGGTT 
GAACCCTTGG ATTCTCTCCC CATAATCCAG TTAAAAGCAG AATCCAACGG ACAGGCGTTT 
ACAGAAAATG GATATGCTCA GGTACAGCCA ACAGTGAATG TCAATCAGAA AGCTCATCCT 
TTACTCCGGC CAACCTCTCC AACTAACCAG TGTGCTAATG GGATGGCTGG CAATGACCAA 
ACACAGTTCC AACAGGATGT TAAAGACCAA GTCACACATC AGAGGCTGCC GACATTGCCT 
GGTATCTCTC ATGAGACCCC TTTACCGGAC CCAGCACAAA TTCTCAGGAA CCTAAATGTA 
GTATGTTCAG GTGGAATTAC AGTGGTTTCT ACCAAAAGTG AAGAGGACGT CTGTTCATCT 
AGTGTTGGAG CATCAGAATT TTCCCCAATA GACAGTGCGC AGAAAAGTTT TAATGATTAT 
GCGATGAACT TCTTTACTAA TCCTACGAAA ACCCTGGTGT CCACAACAAA AGATTCTGAA 
TTGCCTACCT GCAACTGTCT TGATCGAGTT ATACAAAAAG ACAAAGGCCC ATATTATACA 
CACCTTGGGG CAGGACCAAG TGTTGCTGCT GTCAGGGAAA TCATGGAGAA TAGGTATGGT 
CAAAAAGGAA ACGCAATAAG GATAGAAATA GTAGTGTACA CGGGGAAAGA AGGAAAAAGC 
TCTAATGGGT GTCCAGTTGC TAAGTGGGTT TTAAGAAGAA GCAGCGATGA AGAGAAAGTT 
CTTTGTCTCG TTCGACAGCG TGCAGGCCAT CACTGTCCAA CAGCTGTGAT GGTGGTGCTC 
ATCATGGTAT GGGACGGCAT CCCTCTTCCA ATGGCTGACA AATTGTACTC GCAGCTTACG 
GAAAGTCTAA AGTCATACAA TGGTCATCCC ACAGATCGAA GATGCACTCT CAATGAAAAT 
CGTACCTGTA CATGTCAAGG AATTGATCCA GAGACTTGCG GAGCTTCCTT CTCTTTTGGC 
TGTTCTTGGA GTATGTATTT CAATGGCTGC AAATTTGGTA GAAGCCCAAG TCCTAGAAGA 
TTTAGAATTG ATCCAAGTTC TCCCTTACAC CACTACTATG AAAGAATTAC TAAAGGACGT 
AATCCAGAAA GAAGATATAT GAAACCGGAA CCAATCTGTC CGGAACACGA GGCCATGGAA 
AAAAACCTTG AAGATAATTT ACAGAGTTTG GCTACAGAAT TAGCTCCAAT TTATAAGCAG 
TATGCTCCAG TTGCTTACCA AAACCAGGTG GCGCTTGAAC ACGTTGCTCA AGAATGTCGG 
CTTGGCAAGA AAGAAGGTCG TCCTTTCTCT GGGGTCACTG CTTGCCTAGA CTTCTGTGCC 
CACCCCCACA GGGACATTCA CAACATGAAT AATGGGAGCA CGGTGGTCTG CACTTTAACA 
CGAGAAGATA ACCGCTCATT TGGTGTTATT CCTCAAGATG AGCAGCTGCA TGTACTTCCT 
CTTTATAAAC TTTCAGACAC AGATGAGTTT GGCTCAAGGG ACGGAATGGA AGCCAAGATC 
AAATCTGGGG CCATCATGGT CCTCGCACCC CACCGCAGAA AAAGAACACG ATTTACTCAG 
CCAGTTCCTC GTTCTGGGAA GAAGAGAGCA GCAATGATGA CAGAGGTTCT CGCACATAAG 
ATAAGGGCTG TGGAAAAGAA ATTTATTCCT CGAATCAAGC GGAAGAATAA TTCAATGACA 
AACAACAGTA AGGCTTCATC GCTGCCACTT TTAGGGAATA AAACAGAGAC TGTGCAACCT 
GAAATAAAAA GTGAAACTGA ACCCCACCTT ATCTTCAAAG GTTCGGACAA CACTAAAACC 
TACTCACCGA CCCCATCCAT TCCTCACCCA GTGAAAGAGG CACACTTAGC TCCAGGCTTC 
TCCTGGTCCC CTAAGACTGC TTCGGTCCCA ACAGTTCCTT TTAAGAATGA TGCATCAGTT 
TCGTACGGGT TTTCAGAAAG AGGTAGCAAT CCCCACTGCA CAATGCCTTC TGCAAGACAC 
AGTGGTGCTA ATGTAGCTGC TGGGGAGTGT GCTGAAACTG CACAGCCTGG TGAAATGGTT 
CCTCTTTCCA CCTTGTCTAC TCCCATGACA GATTCCCTGG CTTATTCTGA GCCTCCCACT 
GGTCCATCTG AGCAGCTAAC TTCTGATCAG CCAAACCAGC AGCCCCCATT CATCCCTTCT 
CCTCATGAAC TTGCTTCTTC TCTGGCGGAA GAAGACGAGC AGCATTCTGA AGCAGATGAG 
CCTCCATCAG ATGACCCCCT ATCAGACGAC CCCCTGTCAC CTGCTGAGGA GAAACTGCCC 
CACATTGTTG AGTATTGGTC AGACAGTGAG CACATCTTCC TGGATGCCAA TGTTGGTGGG 
GTGGCCATAG CGCCCGCCCA CGGCTCTGTT CTGATCGAGT GCGCCAGGCG AGAGCTTCAC 
GCCACAACTC CTGTCGAACA CCCCAACCGG AATCACCCTA CACGCCTGTC TCTTGTCTTC 
TACCAGCACA AAAACCTGAA TAAGCCCCAA CACGGTTTCG AACTAAACAA GATTAAGTTC 
GAGGCGAAAG AAGCAAAGAA TAAGAAAACT AAAGCCTCCG AGCAGAAAGA CCAGGCAGCT 
AACGAGGGTC CTGAACTGTC CACTGAAGTT AATGAATTTA ACCAGATTCC TTCCCACAAA 
GCGTTAACAT TAACCCATGA CAATGTTGTC ACCGTGTCCC CTTATGCTCT CACACATGTT 
GCGGGGCCCT ATAACCATTG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023987950.1
CDS	111..6617
Translation	MSRSRHARPSKLVRREDLNKKKNSQLKKGSKPANKNVATVKTVSPGKLKQLIQERGVKKKTEPKLPMPVRSLLTRAGAARMNLDRTEVLFQNPESLTCNGFTMALRSTSLSRRLSQPPAVIAKPKKVPPPKNLGKQHECDYKVLTDVGVKHSESDSVPVQDPSIPPDIENLIGIQNASLIKDESQETTQSWSQSVENSKINRSIHSGPAAEVLSGSLEGTRGGKGLFSRETLNDISDSPRMFAQDTLCVPLLQKAALRVTSEGNPSIQLEDLGSRVESLKLSDSYLDPIKSEHDSCPTSSFNKVVPELDLRNCLSIGGSIYPTSLIKLLLAGSEQVTLGAKPDHQEVLKSTPDEQEVPDTTPVLGQAFSAVPHQWEVPGANPVHGEPLGETPGPPEIPGAIPVQGEVFGALLNQQVLGMVGGTASDPDIFPPAPLNPIATCNAFPEWPEPQSTVSSGLEVQGTMQILPSGLGHTPQPSSNSEISSVPPAMAINNIENKKKVHISFLPANTQGFTIAPERGLHHASQGIVHLSQASPSKLEGGSSQISATSMVDINTTVVSRPVSLVSTSSSPSYTTLLPTLEKKKRKRCGVCEPCQQKTNCGECTYCKNRKNSHQICKKRKCEELKKKPSIIVPLEVIKENKRPQREKKPKVLKADFDNKPVNGPKSESMEYSSCGHEEEKRLELNSCPLENVTKNEESMTGIEVEKWTQNKKSHLTDHVKGEFNTIKTEAENLKSSEDDRKKILLTDLLEPQKLFAQTVRNGIKNVHYLPTETNLSFKKINIEELGKAFENNPYKFLKDTANHNNAMSAIATSASCDHLKGRSNVLVIQKPGFNCKSFPDPANCNLNSHPSIHNESDQPKSLEIITNRELKEGSTLQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSENSSPSKSEKDEETQQRTASLLNSCKAILYSVRKDLQDPNLQGGPQSLHHCPSLEKQSSCNTLMFNGQNISKSHNSSSSDQASTKAQEYSIVTNSVSVFIPNSNSSKTDTNKNVAQGRTALDSSKNLHQLTTTTSKSEYCNQSLDSSIKLDSKDDPSCQDTVHSKIEEDVAAQLTQLASIIKFNCIKPEDKKVESTPTSLVAYNVQQNYSQEKGTIEHKPPSSVQNNQGPSVTKQKNTIQKKTKSTPSRDRRKKKPVIISCQENDQKKQEQLAYEYSKLHDIWIASKFQRFGQFGPHDFPILLGKIPPLTKVWKPLTQRSSALEHKKLFPPLTQIKFERYYPELAQEKMKVEPLDSLPIIQLKAESNGQAFTENGYAQVQPTVNVNQKAHPLLRPTSPTNQCANGMAGNDQTQFQQDVKDQVTHQRLPTLPGISHETPLPDPAQILRNLNVVCSGGITVVSTKSEEDVCSSSVGASEFSPIDSAQKSFNDYAMNFFTNPTKTLVSTTKDSELPTCNCLDRVIQKDKGPYYTHLGAGPSVAAVREIMENRYGQKGNAIRIEIVVYTGKEGKSSNGCPVAKWVLRRSSDEEKVLCLVRQRAGHHCPTAVMVVLIMVWDGIPLPMADKLYSQLTESLKSYNGHPTDRRCTLNENRTCTCQGIDPETCGASFSFGCSWSMYFNGCKFGRSPSPRRFRIDPSSPLHHYYERITKGRNPERRYMKPEPICPEHEAMEKNLEDNLQSLATELAPIYKQYAPVAYQNQVALEHVAQECRLGKKEGRPFSGVTACLDFCAHPHRDIHNMNNGSTVVCTLTREDNRSFGVIPQDEQLHVLPLYKLSDTDEFGSRDGMEAKIKSGAIMVLAPHRRKRTRFTQPVPRSGKKRAAMMTEVLAHKIRAVEKKFIPRIKRKNNSMTNNSKASSLPLLGNKTETVQPEIKSETEPHLIFKGSDNTKTYSPTPSIPHPVKEAHLAPGFSWSPKTASVPTVPFKNDASVSYGFSERGSNPHCTMPSARHSGANVAAGECAETAQPGEMVPLSTLSTPMTDSLAYSEPPTGPSEQLTSDQPNQQPPFIPSPHELASSLAEEDEQHSEADEPPSDDPLSDDPLSPAEEKLPHIVEYWSDSEHIFLDANVGGVAIAPAHGSVLIECARRELHATTPVEHPNRNHPTRLSLVFYQHKNLNKPQHGFELNKIKFEAKEAKNKKTKASEQKDQAANEGPELSTEVNEFNQIPSHKALTLTHDNVVTVSPYALTHVAGPYNHWV

Target ORF information:

RefSeq Version	XM_024132182.2
Organism	Physeter catodon(sperm whale)
Definition	Physeter catodon tet methylcytosine dioxygenase 1 (TET1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024132182.2

ORF Insert Sequence:

ATGTCTCGAT CTCGCCATGC AAGGCCTTCC AAATTAGTCA GGAGGGAAGA TCTAAACAAA 
AAGAAAAACA GCCAACTGAA GAAGGGATCT AAGCCAGCCA ACAAAAATGT GGCGACAGTC 
AAGACTGTGA GCCCTGGAAA ATTAAAGCAA TTAATTCAAG AAAGAGGTGT TAAGAAAAAA 
ACAGAACCCA AACTGCCTAT GCCAGTCAGA AGCCTTCTGA CAAGAGCTGG AGCAGCACGA 
ATGAATTTGG ATAGGACTGA GGTTCTTTTT CAGAACCCAG AGTCCTTAAC TTGCAATGGG 
TTTACAATGG CTCTCCGAAG CACCTCTCTT AGCAGACGAC TCTCCCAACC CCCAGCGGTC 
ATAGCCAAAC CCAAGAAAGT TCCACCTCCT AAGAATCTAG GAAAGCAACA TGAGTGTGAT 
TATAAGGTAC TTACTGACGT GGGAGTAAAG CACTCAGAAA GTGATTCAGT CCCAGTGCAA 
GACCCCTCAA TCCCTCCTGA TATAGAGAAT CTAATTGGCA TACAAAATGC ATCATTAATA 
AAAGATGAGA GCCAAGAGAC AACCCAGTCT TGGTCCCAAA GTGTGGAGAA TTCCAAAATA 
AATCGCTCTA TTCATAGTGG CCCTGCAGCA GAGGTTCTTT CTGGGTCATT GGAAGGGACA 
CGTGGTGGTA AAGGACTATT CTCTAGAGAG ACATTGAATG ATATCAGTGA TTCCCCTAGA 
ATGTTTGCTC AGGACACTCT CTGTGTTCCT TTGCTCCAAA AAGCAGCCCT CAGGGTAACC 
TCTGAAGGAA ACCCCAGCAT TCAGTTAGAA GATTTGGGTT CACGAGTAGA ATCTCTTAAG 
CTGTCTGATT CTTACCTGGA TCCCATCAAA AGTGAACATG ATTCCTGCCC CACCTCCAGC 
TTTAATAAGG TTGTACCTGA ATTGGACCTT AGAAACTGTT TGTCTATTGG TGGGTCAATA 
TATCCTACCT CATTAATAAA ACTCCTCTTG GCAGGCTCAG AACAAGTAAC CCTTGGTGCT 
AAACCAGATC ATCAAGAGGT ACTCAAATCT ACCCCAGATG AACAGGAAGT TCCTGATACC 
ACCCCTGTCC TAGGACAGGC CTTTAGTGCT GTCCCACATC AATGGGAAGT TCCTGGTGCT 
AACCCAGTTC ATGGAGAGCC TCTGGGTGAG ACCCCAGGCC CACCAGAGAT TCCTGGTGCT 
ATTCCAGTCC AAGGAGAGGT CTTTGGTGCT CTCTTAAATC AACAAGTCCT CGGTATGGTT 
GGTGGTACTG CCTCAGACCC CGATATCTTT CCTCCTGCAC CTCTTAACCC AATTGCTACC 
TGTAATGCTT TCCCAGAATG GCCTGAGCCC CAGAGCACTG TTTCATCTGG ACTTGAGGTC 
CAGGGCACTA TGCAGATTTT GCCTTCGGGC TTAGGTCACA CTCCTCAACC ATCATCAAAC 
TCAGAGATAA GTTCGGTACC TCCTGCAATG GCTATAAACA ATATAGAAAA TAAGAAGAAG 
GTTCATATAA GCTTTCTGCC AGCTAACACT CAGGGGTTCA CAATAGCCCC TGAGAGAGGA 
CTCCACCATG CTTCACAGGG CATTGTCCAC CTCTCCCAGG CCAGTCCCAG CAAATTGGAA 
GGAGGGAGCT CCCAGATCAG TGCCACCAGC ATGGTTGACA TCAACACCAC AGTGGTGTCT 
AGGCCAGTGT CACTTGTCAG TACCTCCTCT TCTCCTTCCT ACACAACGTT GCTACCTACT 
TTGGAAAAGA AGAAACGAAA GCGATGTGGG GTCTGTGAGC CCTGCCAGCA GAAGACCAAT 
TGTGGTGAAT GTACCTACTG CAAGAATAGA AAGAACAGCC ATCAGATCTG TAAGAAGAGG 
AAATGTGAGG AACTGAAAAA GAAGCCATCC ATCATTGTGC CTTTGGAGGT TATAAAGGAA 
AACAAGAGGC CCCAGAGGGA AAAGAAGCCC AAAGTTTTAA AGGCAGATTT TGACAACAAA 
CCAGTAAATG GCCCCAAGTC AGAATCCATG GAATACAGTA GTTGTGGTCA TGAGGAAGAA 
AAAAGATTGG AATTAAATTC ATGTCCCTTA GAAAATGTAA CTAAAAATGA AGAAAGTATG 
ACAGGTATTG AAGTGGAAAA GTGGACACAA AACAAGAAAT CACACTTAAC TGATCATGTT 
AAAGGAGAAT TTAATACAAT TAAAACAGAA GCTGAAAATT TGAAAAGCTC TGAAGATGAC 
AGGAAAAAAA TCCTACTTAC TGACCTCCTT GAACCTCAAA AATTGTTTGC ACAAACTGTA 
AGAAACGGCA TTAAAAATGT ACACTATTTG CCAACTGAAA CAAATTTGTC TTTTAAAAAA 
ATCAACATTG AAGAACTTGG CAAGGCATTT GAAAACAATC CTTATAAATT CCTGAAAGAC 
ACTGCAAACC ATAACAATGC CATGAGCGCC ATTGCTACTA GTGCGAGCTG TGATCATCTC 
AAGGGGAGAA GTAATGTTTT GGTCATTCAG AAGCCTGGCT TTAATTGTAA GTCTTTTCCA 
GATCCTGCAA ACTGCAACTT AAATAGTCAT CCTAGTATAC ACAATGAAAG TGATCAACCA 
AAATCCCTAG AAATTATAAC AAATAGAGAA CTAAAAGAAG GATCAACACT TCAACCAAGT 
CTTCTATCCC TAATGAAAGA TAGGAGATTA ACGCTAGAAC AAGTAGTGGC CATAGAAGCT 
CTAACACAAC TTTCAGAAGC CCCATCAGAA AATTCCTCAC CGTCGAAGTC AGAGAAGGAT 
GAAGAAACAC AACAGAGAAC AGCTAGTTTG CTTAACAGCT GTAAAGCTAT CCTGTATTCT 
GTAAGAAAAG ACCTCCAAGA CCCAAATTTA CAAGGGGGGC CACAAAGCCT TCATCACTGT 
CCATCTCTGG AAAAGCAAAG TTCATGCAAC ACGTTGATGT TCAATGGCCA AAATATTTCT 
AAGTCACACA ACAGCTCATC TAGTGACCAA GCATCCACAA AGGCACAGGA ATATTCAATA 
GTCACAAATT CAGTATCTGT TTTTATACCA AATTCAAATT CATCTAAAAC CGATACCAAT 
AAAAATGTTG CTCAAGGTAG AACTGCTCTT GACAGTTCCA AAAATTTGCA TCAGTTGACA 
ACAACAACTA GTAAGTCAGA GTATTGTAAC CAGTCACTGG ACAGCAGCAT CAAGTTGGAC 
TCAAAGGATG ATCCCTCATG TCAAGATACA GTTCATAGTA AAATAGAAGA AGATGTTGCT 
GCACAGTTAA CACAACTTGC ATCAATAATT AAATTCAATT GTATAAAACC GGAGGACAAA 
AAAGTTGAAA GTACACCAAC AAGCCTTGTT GCATATAATG TACAGCAAAA CTATAGCCAG 
GAGAAGGGCA CAATAGAGCA TAAACCACCT TCCAGTGTAC AAAATAATCA AGGACCTTCA 
GTAACAAAGC AAAAGAACAC AATCCAGAAA AAGACAAAAT CTACCCCATC CAGAGATCGA 
CGGAAAAAGA AGCCTGTGAT CATAAGTTGT CAAGAAAATG ACCAGAAAAA ACAGGAACAG 
TTGGCATATG AATATAGTAA GTTACATGAC ATTTGGATAG CATCTAAATT TCAAAGATTT 
GGTCAGTTTG GTCCACATGA CTTTCCTATT CTATTGGGAA AAATTCCTCC CCTAACCAAA 
GTATGGAAAC CACTGACTCA AAGGAGCTCA GCATTAGAAC ACAAAAAATT ATTTCCTCCA 
CTCACTCAAA TAAAATTTGA GCGGTATTAT CCTGAATTAG CCCAGGAGAA GATGAAGGTT 
GAACCCTTGG ATTCTCTCCC CATAATCCAG TTAAAAGCAG AATCCAACGG ACAGGCGTTT 
ACAGAAAATG GATATGCTCA GGTACAGCCA ACAGTGAATG TCAATCAGAA AGCTCATCCT 
TTACTCCGGC CAACCTCTCC AACTAACCAG TGTGCTAATG GGATGGCTGG CAATGACCAA 
ACACAGTTCC AACAGGATGT TAAAGACCAA GTCACACATC AGAGGCTGCC GACATTGCCT 
GGTATCTCTC ATGAGACCCC TTTACCGGAC CCAGCACAAA TTCTCAGGAA CCTAAATGTA 
GTATGTTCAG GTGGAATTAC AGTGGTTTCT ACCAAAAGTG AAGAGGACGT CTGTTCATCT 
AGTGTTGGAG CATCAGAATT TTCCCCAATA GACAGTGCGC AGAAAAGTTT TAATGATTAT 
GCGATGAACT TCTTTACTAA TCCTACGAAA ACCCTGGTGT CCACAACAAA AGATTCTGAA 
TTGCCTACCT GCAACTGTCT TGATCGAGTT ATACAAAAAG ACAAAGGCCC ATATTATACA 
CACCTTGGGG CAGGACCAAG TGTTGCTGCT GTCAGGGAAA TCATGGAGAA TAGGTATGGT 
CAAAAAGGAA ACGCAATAAG GATAGAAATA GTAGTGTACA CGGGGAAAGA AGGAAAAAGC 
TCTAATGGGT GTCCAGTTGC TAAGTGGGTT TTAAGAAGAA GCAGCGATGA AGAGAAAGTT 
CTTTGTCTCG TTCGACAGCG TGCAGGCCAT CACTGTCCAA CAGCTGTGAT GGTGGTGCTC 
ATCATGGTAT GGGACGGCAT CCCTCTTCCA ATGGCTGACA AATTGTACTC GCAGCTTACG 
GAAAGTCTAA AGTCATACAA TGGTCATCCC ACAGATCGAA GATGCACTCT CAATGAAAAT 
CGTACCTGTA CATGTCAAGG AATTGATCCA GAGACTTGCG GAGCTTCCTT CTCTTTTGGC 
TGTTCTTGGA GTATGTATTT CAATGGCTGC AAATTTGGTA GAAGCCCAAG TCCTAGAAGA 
TTTAGAATTG ATCCAAGTTC TCCCTTACAC CACTACTATG AAAGAATTAC TAAAGGACGT 
AATCCAGAAA GAAGATATAT GAAACCGGAA CCAATCTGTC CGGAACACGA GGCCATGGAA 
AAAAACCTTG AAGATAATTT ACAGAGTTTG GCTACAGAAT TAGCTCCAAT TTATAAGCAG 
TATGCTCCAG TTGCTTACCA AAACCAGGTG GCGCTTGAAC ACGTTGCTCA AGAATGTCGG 
CTTGGCAAGA AAGAAGGTCG TCCTTTCTCT GGGGTCACTG CTTGCCTAGA CTTCTGTGCC 
CACCCCCACA GGGACATTCA CAACATGAAT AATGGGAGCA CGGTGGTCTG CACTTTAACA 
CGAGAAGATA ACCGCTCATT TGGTGTTATT CCTCAAGATG AGCAGCTGCA TGTACTTCCT 
CTTTATAAAC TTTCAGACAC AGATGAGTTT GGCTCAAGGG ACGGAATGGA AGCCAAGATC 
AAATCTGGGG CCATCATGGT CCTCGCACCC CACCGCAGAA AAAGAACACG ATTTACTCAG 
CCAGTTCCTC GTTCTGGGAA GAAGAGAGCA GCAATGATGA CAGAGGTTCT CGCACATAAG 
ATAAGGGCTG TGGAAAAGAA ATTTATTCCT CGAATCAAGC GGAAGAATAA TTCAATGACA 
AACAACAGTA AGGCTTCATC GCTGCCACTT TTAGGGAATA AAACAGAGAC TGTGCAACCT 
GAAATAAAAA GTGAAACTGA ACCCCACCTT ATCTTCAAAG GTTCGGACAA CACTAAAACC 
TACTCACCGA CCCCATCCAT TCCTCACCCA GTGAAAGAGG CACACTTAGC TCCAGGCTTC 
TCCTGGTCCC CTAAGACTGC TTCGGTCCCA ACAGTTCCTT TTAAGAATGA TGCATCAGTT 
TCGTACGGGT TTTCAGAAAG AGGTAGCAAT CCCCACTGCA CAATGCCTTC TGCAAGACAC 
AGTGGTGCTA ATGTAGCTGC TGGGGAGTGT GCTGAAACTG CACAGCCTGG TGAAATGGTT 
CCTCTTTCCA CCTTGTCTAC TCCCATGACA GATTCCCTGG CTTATTCTGA GCCTCCCACT 
GGTCCATCTG AGCAGCTAAC TTCTGATCAG CCAAACCAGC AGCCCCCATT CATCCCTTCT 
CCTCATGAAC TTGCTTCTTC TCTGGCGGAA GAAGACGAGC AGCATTCTGA AGCAGATGAG 
CCTCCATCAG ATGACCCCCT ATCAGACGAC CCCCTGTCAC CTGCTGAGGA GAAACTGCCC 
CACATTGTTG AGTATTGGTC AGACAGTGAG CACATCTTCC TGGATGCCAA TGTTGGTGGG 
GTGGCCATAG CGCCCGCCCA CGGCTCTGTT CTGATCGAGT GCGCCAGGCG AGAGCTTCAC 
GCCACAACTC CTGTCGAACA CCCCAACCGG AATCACCCTA CACGCCTGTC TCTTGTCTTC 
TACCAGCACA AAAACCTGAA TAAGCCCCAA CACGGTTTCG AACTAAACAA GATTAAGTTC 
GAGGCGAAAG AAGCAAAGAA TAAGAAAACT AAAGCCTCCG AGCAGAAAGA CCAGGCAGCT 
AACGAGGGTC CTGAACTGTC CACTGAAGTT AATGAATTTA ACCAGATTCC TTCCCACAAA 
GCGTTAACAT TAACCCATGA CAATGTTGTC ACCGTGTCCC CTTATGCTCT CACACATGTT 
GCGGGGCCCT ATAACCATTG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TET1 cDNA ORF clone, Physeter catodon(sperm whale)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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