GPLD1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	GPLD1
Entrez Gene ID	100931811
Full Name	glycosylphosphatidylinositol specific phospholipase D1
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003760134.2	XP_003760182.1	phosphatidylinositol-glycan-specific phospholipase D
XM_003760134.3	XP_003760182.1	phosphatidylinositol-glycan-specific phospholipase D isoform X1
XM_023496317.1	XP_023352085.1	phosphatidylinositol-glycan-specific phospholipase D isoform X2
XM_031946779.1	XP_031802639.1	phosphatidylinositol-glycan-specific phospholipase D isoform X1
XM_003760134.4	XP_003760182.1	phosphatidylinositol-glycan-specific phospholipase D isoform X2

Pathways from BioSystems
Gene Ontology

The following GPLD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPLD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa121791	XM_003760134.3 Latest version!	Sarcophilus harrisii glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSa158692	XM_023496317.1 Latest version!	Sarcophilus harrisii glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSa221519	XM_031946779.1 Latest version!	Sarcophilus harrisii glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSa221520	XM_003760134.4 Latest version!	Sarcophilus harrisii glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OSa121791	XM_003760134.2	Sarcophilus harrisii glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPLD1 ( XM_003760134.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003760182.1 Sarcophilus harrisii glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), mRNA.

Price & Availability↑

CloneID	OSa121791
Clone ID Related Accession (Same CDS sequence)	XM_003760134.2 , XM_003760134.3
Accession Version	XM_003760134.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	phosphatidylinositol-glycan-specific phospholipase D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 15, 2015 this sequence version replaced XM_003760134.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCTGCTT TCAGGAGCTG GTTTCTTGTG CTGATCATCC TGTTCCATTT GTCTCAAAGA 
AGTACATCCT GTGGTATTTC AACCCACATA GAAATAGCAC ACAGAGCACT GGAATTTTTC 
AGCCAACAAG ATGGCGCTAT TAACTATAAA AAGCTGTTAT TAGAACATCA GGATGCTTAT 
CAGGCTGGGA GTGTGTTTCC TGATGCTTTT TATCCTGACA TCTGCAAAGG AGGAATTTTT 
CACGATGTAT CTGAAGATAC TCACTGGACT CCATTTCTCA ATGCAAGCAT CCATTACATC 
CGACAAAATT ATCCTCTTCC TTGGGAGAAG GATACTGAGA AATTGGTGGC TTTCCTGTTT 
GGCATTATGT CACACATGGT GGCAGATGTG AGCTGGCATA GCCTGGGCAT TGAACAAGGA 
TTCTTGCAGA CAATGGGAGA AATTGATTTT CATGGCTCTT ACCAAGATGC TCATTCTGTT 
GGTGATTTTG GAGGAGATGT GTTGAGCCAT TTTGAACTTA ATTTAAATTA CCTCGGGCGG 
AAATGGTATA TACCTGTCAA AGACCTAATG GGAATCTACA AGCAGCTCTA TGGCAAGGAA 
GTCATAACGA AAAAAGCAAT AATTGACTGT TCATATCTTC AGTTCTTAGA ATTGTATGGT 
GAGATATTGG TTCTTTCCAA GCTTTACCCA AATTTTGCAA AAAAGTCCCC ATTTTTGGTG 
GAACAATTAC AAGATTATTT CCTTGGTGGA CTAGATGACA TGGCATTCTG GTCCACTAAT 
ATGTTTCATC TAACAAGCTA CATGTTACAG AATGGGACCA GTGACTGTTC CCTACCTGAG 
AATCCTCTGT TCATGGCATG TGGTCCCAAA CATAATGGCA GCCTTGGAAT AAAACTGCAG 
AAAAATAAAT GTCATGCAAA TGTAAATGTA TCCTTAGCTG AAAATAGTAA AGGAAATATA 
AATTACACAG AAAGAGGAGT CTTCTACAGT GTGGATTCGT GGGCCCAAGA TTCGGTCTCC 
TTTTTGTATT TGACTTTCAC CAAGAAGTTA AGGAAAATGT TTGCAAGTGA ACAGCCTCAC 
CAGCATAATT ATGTGTCCAG TCCCATAGCA TCTTACTTTC TGTCACTTCC CTATGTGAAG 
CTTGGATGGT CAATGACTTC AGCAGACCTC AACCAGGATG GCCATGAGGA TCTGGTGGTA 
GGAGCACCAG GTTATAGCAG ATCAGGCCAT ATTCAGGTGG GACGAGTATA TCTCATCTAC 
AGCAATGCTA TAGGTTTGCC TCCCGTAGAC TTGGATTTGG ACAAAGAAGC ACATGTAATT 
CTAGAGGGCT TTCAGCCCTC AGGTCGTTTT GGGTCTTCCG TGGCAGTATT GGATTTCAAC 
AAGGATGGGC TGCCTGACCT AGCAGTGGGA GCCCCCTCGG TGGGAGCTGA GAAACTCACT 
TACAATGGAG CAGTGTATGT CTTTTTTGGT ACCAGAAAAG GAAAAATATC TTCTAAACCA 
AATGTCACCA TTTTGTGCAA GGACACATAT TGTAACCTTG GCTGGACGCT TTTGGCAGCA 
GATATTAATG GAGATGGTGC TCCTGACCTG GTGGTTGGGT CACCCTATGC ACCCAGTGGA 
GGGAAGCAAA GGGGGATTGT GGCTGCATTC TATTCGGATT CCAAACAAAA TGTCAAAGAT 
CAACTGAATG TTGAGGAAGC TAATTGGATG ATAAAGGGGA AAGATGACTA TGGCTGGTTT 
GGATATTCAA TTCATAGTCT CAGCTTAAAG AACAGAACCT TGTTATTAGT TGGGAGTCCA 
ACCTGGAAGA ATGTGAGTGG GTTAGACAGC GTGTTCTACA TCCGTGATGA GAAACAAAGC 
ATTGGAAAGG TCTATGGCTA TTACCCACCG AGTAGACAAA GTTGGTTTAC AATTTCTGGA 
GATGAGGCAA TGGGAAAACT GGGCACCTCA CTCTCAAGTG GCGTTGTGAA AGTCAATGGA 
ACCCTAAGAC AAGTGCTGCT GGTTGGTGCC CCAACTCGTG ACGACGTGTC CAAAATGGCA 
TTCTTATCCA TGACCCTGCA CCAGGGAGGC AGCACAAGGG TATATGACTT GACAAATGAG 
ACAGGGCCCT CTCTGCTTAG CACCTTCAGT GGGGACAGGC ATTTCTCTCG CTTTGGAGGA 
CTGGTGCACT TAAGTGACCT GGACAGTGAT GGATTAGATG AGATCATCAT ATCAGCCCCT 
CTGAGAACAG GGGACTTAAC AGCTGGGCTT CTCGGTGGGG AGGATGGCCG AGTTTACATC 
TACAATGGCA AGGAAGCTCC CCTTGGAGAT GTCACAAGTA AATGCAAATC CTGGATCTCT 
CCTTGTCCTG AAGAAAAGGC CCAGCATGTA CTGATTTCTC TAGAAGAAAA TTCAAGATTC 
GGGAGCTCTC TGGTCACAGT GAAGTCAAAG ACAAAGAATC AAGTTGTTGT TGCTGCTGGG 
AGGAGCTCTA TGGGAGCCAG ACTGGCTGGG ACGCTTCACA TCTACAATCT GGGAGTTGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003760182.1
CDS	1..2523
Translation	MSAFRSWFLVLIILFHLSQRSTSCGISTHIEIAHRALEFFSQQDGAINYKKLLLEHQDAYQAGSVFPDAFYPDICKGGIFHDVSEDTHWTPFLNASIHYIRQNYPLPWEKDTEKLVAFLFGIMSHMVADVSWHSLGIEQGFLQTMGEIDFHGSYQDAHSVGDFGGDVLSHFELNLNYLGRKWYIPVKDLMGIYKQLYGKEVITKKAIIDCSYLQFLELYGEILVLSKLYPNFAKKSPFLVEQLQDYFLGGLDDMAFWSTNMFHLTSYMLQNGTSDCSLPENPLFMACGPKHNGSLGIKLQKNKCHANVNVSLAENSKGNINYTERGVFYSVDSWAQDSVSFLYLTFTKKLRKMFASEQPHQHNYVSSPIASYFLSLPYVKLGWSMTSADLNQDGHEDLVVGAPGYSRSGHIQVGRVYLIYSNAIGLPPVDLDLDKEAHVILEGFQPSGRFGSSVAVLDFNKDGLPDLAVGAPSVGAEKLTYNGAVYVFFGTRKGKISSKPNVTILCKDTYCNLGWTLLAADINGDGAPDLVVGSPYAPSGGKQRGIVAAFYSDSKQNVKDQLNVEEANWMIKGKDDYGWFGYSIHSLSLKNRTLLLVGSPTWKNVSGLDSVFYIRDEKQSIGKVYGYYPPSRQSWFTISGDEAMGKLGTSLSSGVVKVNGTLRQVLLVGAPTRDDVSKMAFLSMTLHQGGSTRVYDLTNETGPSLLSTFSGDRHFSRFGGLVHLSDLDSDGLDEIIISAPLRTGDLTAGLLGGEDGRVYIYNGKEAPLGDVTSKCKSWISPCPEEKAQHVLISLEENSRFGSSLVTVKSKTKNQVVVAAGRSSMGARLAGTLHIYNLGVD

Target ORF information:

RefSeq Version	XM_003760134.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003760134.2

ORF Insert Sequence:

ATGTCTGCTT TCAGGAGCTG GTTTCTTGTG CTGATCATCC TGTTCCATTT GTCTCAAAGA 
AGTACATCCT GTGGTATTTC AACCCACATA GAAATAGCAC ACAGAGCACT GGAATTTTTC 
AGCCAACAAG ATGGCGCTAT TAACTATAAA AAGCTGTTAT TAGAACATCA GGATGCTTAT 
CAGGCTGGGA GTGTGTTTCC TGATGCTTTT TATCCTGACA TCTGCAAAGG AGGAATTTTT 
CACGATGTAT CTGAAGATAC TCACTGGACT CCATTTCTCA ATGCAAGCAT CCATTACATC 
CGACAAAATT ATCCTCTTCC TTGGGAGAAG GATACTGAGA AATTGGTGGC TTTCCTGTTT 
GGCATTATGT CACACATGGT GGCAGATGTG AGCTGGCATA GCCTGGGCAT TGAACAAGGA 
TTCTTGCAGA CAATGGGAGA AATTGATTTT CATGGCTCTT ACCAAGATGC TCATTCTGTT 
GGTGATTTTG GAGGAGATGT GTTGAGCCAT TTTGAACTTA ATTTAAATTA CCTCGGGCGG 
AAATGGTATA TACCTGTCAA AGACCTAATG GGAATCTACA AGCAGCTCTA TGGCAAGGAA 
GTCATAACGA AAAAAGCAAT AATTGACTGT TCATATCTTC AGTTCTTAGA ATTGTATGGT 
GAGATATTGG TTCTTTCCAA GCTTTACCCA AATTTTGCAA AAAAGTCCCC ATTTTTGGTG 
GAACAATTAC AAGATTATTT CCTTGGTGGA CTAGATGACA TGGCATTCTG GTCCACTAAT 
ATGTTTCATC TAACAAGCTA CATGTTACAG AATGGGACCA GTGACTGTTC CCTACCTGAG 
AATCCTCTGT TCATGGCATG TGGTCCCAAA CATAATGGCA GCCTTGGAAT AAAACTGCAG 
AAAAATAAAT GTCATGCAAA TGTAAATGTA TCCTTAGCTG AAAATAGTAA AGGAAATATA 
AATTACACAG AAAGAGGAGT CTTCTACAGT GTGGATTCGT GGGCCCAAGA TTCGGTCTCC 
TTTTTGTATT TGACTTTCAC CAAGAAGTTA AGGAAAATGT TTGCAAGTGA ACAGCCTCAC 
CAGCATAATT ATGTGTCCAG TCCCATAGCA TCTTACTTTC TGTCACTTCC CTATGTGAAG 
CTTGGATGGT CAATGACTTC AGCAGACCTC AACCAGGATG GCCATGAGGA TCTGGTGGTA 
GGAGCACCAG GTTATAGCAG ATCAGGCCAT ATTCAGGTGG GACGAGTATA TCTCATCTAC 
AGCAATGCTA TAGGTTTGCC TCCCGTAGAC TTGGATTTGG ACAAAGAAGC ACATGTAATT 
CTAGAGGGCT TTCAGCCCTC AGGTCGTTTT GGGTCTTCCG TGGCAGTATT GGATTTCAAC 
AAGGATGGGC TGCCTGACCT AGCAGTGGGA GCCCCCTCGG TGGGAGCTGA GAAACTCACT 
TACAATGGAG CAGTGTATGT CTTTTTTGGT ACCAGAAAAG GAAAAATATC TTCTAAACCA 
AATGTCACCA TTTTGTGCAA GGACACATAT TGTAACCTTG GCTGGACGCT TTTGGCAGCA 
GATATTAATG GAGATGGTGC TCCTGACCTG GTGGTTGGGT CACCCTATGC ACCCAGTGGA 
GGGAAGCAAA GGGGGATTGT GGCTGCATTC TATTCGGATT CCAAACAAAA TGTCAAAGAT 
CAACTGAATG TTGAGGAAGC TAATTGGATG ATAAAGGGGA AAGATGACTA TGGCTGGTTT 
GGATATTCAA TTCATAGTCT CAGCTTAAAG AACAGAACCT TGTTATTAGT TGGGAGTCCA 
ACCTGGAAGA ATGTGAGTGG GTTAGACAGC GTGTTCTACA TCCGTGATGA GAAACAAAGC 
ATTGGAAAGG TCTATGGCTA TTACCCACCG AGTAGACAAA GTTGGTTTAC AATTTCTGGA 
GATGAGGCAA TGGGAAAACT GGGCACCTCA CTCTCAAGTG GCGTTGTGAA AGTCAATGGA 
ACCCTAAGAC AAGTGCTGCT GGTTGGTGCC CCAACTCGTG ACGACGTGTC CAAAATGGCA 
TTCTTATCCA TGACCCTGCA CCAGGGAGGC AGCACAAGGG TATATGACTT GACAAATGAG 
ACAGGGCCCT CTCTGCTTAG CACCTTCAGT GGGGACAGGC ATTTCTCTCG CTTTGGAGGA 
CTGGTGCACT TAAGTGACCT GGACAGTGAT GGATTAGATG AGATCATCAT ATCAGCCCCT 
CTGAGAACAG GGGACTTAAC AGCTGGGCTT CTCGGTGGGG AGGATGGCCG AGTTTACATC 
TACAATGGCA AGGAAGCTCC CCTTGGAGAT GTCACAAGTA AATGCAAATC CTGGATCTCT 
CCTTGTCCTG AAGAAAAGGC CCAGCATGTA CTGATTTCTC TAGAAGAAAA TTCAAGATTC 
GGGAGCTCTC TGGTCACAGT GAAGTCAAAG ACAAAGAATC AAGTTGTTGT TGCTGCTGGG 
AGGAGCTCTA TGGGAGCCAG ACTGGCTGGG ACGCTTCACA TCTACAATCT GGGAGTTGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPLD1 ( XM_003760134.3 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003760182.1 Sarcophilus harrisii glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa121791
Clone ID Related Accession (Same CDS sequence)	XM_003760134.2 , XM_003760134.3
Accession Version	XM_003760134.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	phosphatidylinositol-glycan-specific phospholipase D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_003760134.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT TCAGGAGCTG GTTTCTTGTG CTGATCATCC TGTTCCATTT GTCTCAAAGA 
AGTACATCCT GTGGTATTTC AACCCACATA GAAATAGCAC ACAGAGCACT GGAATTTTTC 
AGCCAACAAG ATGGCGCTAT TAACTATAAA AAGCTGTTAT TAGAACATCA GGATGCTTAT 
CAGGCTGGGA GTGTGTTTCC TGATGCTTTT TATCCTGACA TCTGCAAAGG AGGAATTTTT 
CACGATGTAT CTGAAGATAC TCACTGGACT CCATTTCTCA ATGCAAGCAT CCATTACATC 
CGACAAAATT ATCCTCTTCC TTGGGAGAAG GATACTGAGA AATTGGTGGC TTTCCTGTTT 
GGCATTATGT CACACATGGT GGCAGATGTG AGCTGGCATA GCCTGGGCAT TGAACAAGGA 
TTCTTGCAGA CAATGGGAGA AATTGATTTT CATGGCTCTT ACCAAGATGC TCATTCTGTT 
GGTGATTTTG GAGGAGATGT GTTGAGCCAT TTTGAACTTA ATTTAAATTA CCTCGGGCGG 
AAATGGTATA TACCTGTCAA AGACCTAATG GGAATCTACA AGCAGCTCTA TGGCAAGGAA 
GTCATAACGA AAAAAGCAAT AATTGACTGT TCATATCTTC AGTTCTTAGA ATTGTATGGT 
GAGATATTGG TTCTTTCCAA GCTTTACCCA AATTTTGCAA AAAAGTCCCC ATTTTTGGTG 
GAACAATTAC AAGATTATTT CCTTGGTGGA CTAGATGACA TGGCATTCTG GTCCACTAAT 
ATGTTTCATC TAACAAGCTA CATGTTACAG AATGGGACCA GTGACTGTTC CCTACCTGAG 
AATCCTCTGT TCATGGCATG TGGTCCCAAA CATAATGGCA GCCTTGGAAT AAAACTGCAG 
AAAAATAAAT GTCATGCAAA TGTAAATGTA TCCTTAGCTG AAAATAGTAA AGGAAATATA 
AATTACACAG AAAGAGGAGT CTTCTACAGT GTGGATTCGT GGGCCCAAGA TTCGGTCTCC 
TTTTTGTATT TGACTTTCAC CAAGAAGTTA AGGAAAATGT TTGCAAGTGA ACAGCCTCAC 
CAGCATAATT ATGTGTCCAG TCCCATAGCA TCTTACTTTC TGTCACTTCC CTATGTGAAG 
CTTGGATGGT CAATGACTTC AGCAGACCTC AACCAGGATG GCCATGAGGA TCTGGTGGTA 
GGAGCACCAG GTTATAGCAG ATCAGGCCAT ATTCAGGTGG GACGAGTATA TCTCATCTAC 
AGCAATGCTA TAGGTTTGCC TCCCGTAGAC TTGGATTTGG ACAAAGAAGC ACATGTAATT 
CTAGAGGGCT TTCAGCCCTC AGGTCGTTTT GGGTCTTCCG TGGCAGTATT GGATTTCAAC 
AAGGATGGGC TGCCTGACCT AGCAGTGGGA GCCCCCTCGG TGGGAGCTGA GAAACTCACT 
TACAATGGAG CAGTGTATGT CTTTTTTGGT ACCAGAAAAG GAAAAATATC TTCTAAACCA 
AATGTCACCA TTTTGTGCAA GGACACATAT TGTAACCTTG GCTGGACGCT TTTGGCAGCA 
GATATTAATG GAGATGGTGC TCCTGACCTG GTGGTTGGGT CACCCTATGC ACCCAGTGGA 
GGGAAGCAAA GGGGGATTGT GGCTGCATTC TATTCGGATT CCAAACAAAA TGTCAAAGAT 
CAACTGAATG TTGAGGAAGC TAATTGGATG ATAAAGGGGA AAGATGACTA TGGCTGGTTT 
GGATATTCAA TTCATAGTCT CAGCTTAAAG AACAGAACCT TGTTATTAGT TGGGAGTCCA 
ACCTGGAAGA ATGTGAGTGG GTTAGACAGC GTGTTCTACA TCCGTGATGA GAAACAAAGC 
ATTGGAAAGG TCTATGGCTA TTACCCACCG AGTAGACAAA GTTGGTTTAC AATTTCTGGA 
GATGAGGCAA TGGGAAAACT GGGCACCTCA CTCTCAAGTG GCGTTGTGAA AGTCAATGGA 
ACCCTAAGAC AAGTGCTGCT GGTTGGTGCC CCAACTCGTG ACGACGTGTC CAAAATGGCA 
TTCTTATCCA TGACCCTGCA CCAGGGAGGC AGCACAAGGG TATATGACTT GACAAATGAG 
ACAGGGCCCT CTCTGCTTAG CACCTTCAGT GGGGACAGGC ATTTCTCTCG CTTTGGAGGA 
CTGGTGCACT TAAGTGACCT GGACAGTGAT GGATTAGATG AGATCATCAT ATCAGCCCCT 
CTGAGAACAG GGGACTTAAC AGCTGGGCTT CTCGGTGGGG AGGATGGCCG AGTTTACATC 
TACAATGGCA AGGAAGCTCC CCTTGGAGAT GTCACAAGTA AATGCAAATC CTGGATCTCT 
CCTTGTCCTG AAGAAAAGGC CCAGCATGTA CTGATTTCTC TAGAAGAAAA TTCAAGATTC 
GGGAGCTCTC TGGTCACAGT GAAGTCAAAG ACAAAGAATC AAGTTGTTGT TGCTGCTGGG 
AGGAGCTCTA TGGGAGCCAG ACTGGCTGGG ACGCTTCACA TCTACAATCT GGGAGTTGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003760182.1
CDS	67..2589
Translation	MSAFRSWFLVLIILFHLSQRSTSCGISTHIEIAHRALEFFSQQDGAINYKKLLLEHQDAYQAGSVFPDAFYPDICKGGIFHDVSEDTHWTPFLNASIHYIRQNYPLPWEKDTEKLVAFLFGIMSHMVADVSWHSLGIEQGFLQTMGEIDFHGSYQDAHSVGDFGGDVLSHFELNLNYLGRKWYIPVKDLMGIYKQLYGKEVITKKAIIDCSYLQFLELYGEILVLSKLYPNFAKKSPFLVEQLQDYFLGGLDDMAFWSTNMFHLTSYMLQNGTSDCSLPENPLFMACGPKHNGSLGIKLQKNKCHANVNVSLAENSKGNINYTERGVFYSVDSWAQDSVSFLYLTFTKKLRKMFASEQPHQHNYVSSPIASYFLSLPYVKLGWSMTSADLNQDGHEDLVVGAPGYSRSGHIQVGRVYLIYSNAIGLPPVDLDLDKEAHVILEGFQPSGRFGSSVAVLDFNKDGLPDLAVGAPSVGAEKLTYNGAVYVFFGTRKGKISSKPNVTILCKDTYCNLGWTLLAADINGDGAPDLVVGSPYAPSGGKQRGIVAAFYSDSKQNVKDQLNVEEANWMIKGKDDYGWFGYSIHSLSLKNRTLLLVGSPTWKNVSGLDSVFYIRDEKQSIGKVYGYYPPSRQSWFTISGDEAMGKLGTSLSSGVVKVNGTLRQVLLVGAPTRDDVSKMAFLSMTLHQGGSTRVYDLTNETGPSLLSTFSGDRHFSRFGGLVHLSDLDSDGLDEIIISAPLRTGDLTAGLLGGEDGRVYIYNGKEAPLGDVTSKCKSWISPCPEEKAQHVLISLEENSRFGSSLVTVKSKTKNQVVVAAGRSSMGARLAGTLHIYNLGVD

Target ORF information:

RefSeq Version	XM_003760134.3
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003760134.3

ORF Insert Sequence:

ATGTCTGCTT TCAGGAGCTG GTTTCTTGTG CTGATCATCC TGTTCCATTT GTCTCAAAGA 
AGTACATCCT GTGGTATTTC AACCCACATA GAAATAGCAC ACAGAGCACT GGAATTTTTC 
AGCCAACAAG ATGGCGCTAT TAACTATAAA AAGCTGTTAT TAGAACATCA GGATGCTTAT 
CAGGCTGGGA GTGTGTTTCC TGATGCTTTT TATCCTGACA TCTGCAAAGG AGGAATTTTT 
CACGATGTAT CTGAAGATAC TCACTGGACT CCATTTCTCA ATGCAAGCAT CCATTACATC 
CGACAAAATT ATCCTCTTCC TTGGGAGAAG GATACTGAGA AATTGGTGGC TTTCCTGTTT 
GGCATTATGT CACACATGGT GGCAGATGTG AGCTGGCATA GCCTGGGCAT TGAACAAGGA 
TTCTTGCAGA CAATGGGAGA AATTGATTTT CATGGCTCTT ACCAAGATGC TCATTCTGTT 
GGTGATTTTG GAGGAGATGT GTTGAGCCAT TTTGAACTTA ATTTAAATTA CCTCGGGCGG 
AAATGGTATA TACCTGTCAA AGACCTAATG GGAATCTACA AGCAGCTCTA TGGCAAGGAA 
GTCATAACGA AAAAAGCAAT AATTGACTGT TCATATCTTC AGTTCTTAGA ATTGTATGGT 
GAGATATTGG TTCTTTCCAA GCTTTACCCA AATTTTGCAA AAAAGTCCCC ATTTTTGGTG 
GAACAATTAC AAGATTATTT CCTTGGTGGA CTAGATGACA TGGCATTCTG GTCCACTAAT 
ATGTTTCATC TAACAAGCTA CATGTTACAG AATGGGACCA GTGACTGTTC CCTACCTGAG 
AATCCTCTGT TCATGGCATG TGGTCCCAAA CATAATGGCA GCCTTGGAAT AAAACTGCAG 
AAAAATAAAT GTCATGCAAA TGTAAATGTA TCCTTAGCTG AAAATAGTAA AGGAAATATA 
AATTACACAG AAAGAGGAGT CTTCTACAGT GTGGATTCGT GGGCCCAAGA TTCGGTCTCC 
TTTTTGTATT TGACTTTCAC CAAGAAGTTA AGGAAAATGT TTGCAAGTGA ACAGCCTCAC 
CAGCATAATT ATGTGTCCAG TCCCATAGCA TCTTACTTTC TGTCACTTCC CTATGTGAAG 
CTTGGATGGT CAATGACTTC AGCAGACCTC AACCAGGATG GCCATGAGGA TCTGGTGGTA 
GGAGCACCAG GTTATAGCAG ATCAGGCCAT ATTCAGGTGG GACGAGTATA TCTCATCTAC 
AGCAATGCTA TAGGTTTGCC TCCCGTAGAC TTGGATTTGG ACAAAGAAGC ACATGTAATT 
CTAGAGGGCT TTCAGCCCTC AGGTCGTTTT GGGTCTTCCG TGGCAGTATT GGATTTCAAC 
AAGGATGGGC TGCCTGACCT AGCAGTGGGA GCCCCCTCGG TGGGAGCTGA GAAACTCACT 
TACAATGGAG CAGTGTATGT CTTTTTTGGT ACCAGAAAAG GAAAAATATC TTCTAAACCA 
AATGTCACCA TTTTGTGCAA GGACACATAT TGTAACCTTG GCTGGACGCT TTTGGCAGCA 
GATATTAATG GAGATGGTGC TCCTGACCTG GTGGTTGGGT CACCCTATGC ACCCAGTGGA 
GGGAAGCAAA GGGGGATTGT GGCTGCATTC TATTCGGATT CCAAACAAAA TGTCAAAGAT 
CAACTGAATG TTGAGGAAGC TAATTGGATG ATAAAGGGGA AAGATGACTA TGGCTGGTTT 
GGATATTCAA TTCATAGTCT CAGCTTAAAG AACAGAACCT TGTTATTAGT TGGGAGTCCA 
ACCTGGAAGA ATGTGAGTGG GTTAGACAGC GTGTTCTACA TCCGTGATGA GAAACAAAGC 
ATTGGAAAGG TCTATGGCTA TTACCCACCG AGTAGACAAA GTTGGTTTAC AATTTCTGGA 
GATGAGGCAA TGGGAAAACT GGGCACCTCA CTCTCAAGTG GCGTTGTGAA AGTCAATGGA 
ACCCTAAGAC AAGTGCTGCT GGTTGGTGCC CCAACTCGTG ACGACGTGTC CAAAATGGCA 
TTCTTATCCA TGACCCTGCA CCAGGGAGGC AGCACAAGGG TATATGACTT GACAAATGAG 
ACAGGGCCCT CTCTGCTTAG CACCTTCAGT GGGGACAGGC ATTTCTCTCG CTTTGGAGGA 
CTGGTGCACT TAAGTGACCT GGACAGTGAT GGATTAGATG AGATCATCAT ATCAGCCCCT 
CTGAGAACAG GGGACTTAAC AGCTGGGCTT CTCGGTGGGG AGGATGGCCG AGTTTACATC 
TACAATGGCA AGGAAGCTCC CCTTGGAGAT GTCACAAGTA AATGCAAATC CTGGATCTCT 
CCTTGTCCTG AAGAAAAGGC CCAGCATGTA CTGATTTCTC TAGAAGAAAA TTCAAGATTC 
GGGAGCTCTC TGGTCACAGT GAAGTCAAAG ACAAAGAATC AAGTTGTTGT TGCTGCTGGG 
AGGAGCTCTA TGGGAGCCAG ACTGGCTGGG ACGCTTCACA TCTACAATCT GGGAGTTGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPLD1 ( XM_023496317.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023352085.1 Sarcophilus harrisii glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa158692
Clone ID Related Accession (Same CDS sequence)	XM_023496317.1
Accession Version	XM_023496317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	phosphatidylinositol-glycan-specific phospholipase D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823352.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT TCAGGAGCTG GTTTCTTGTG CTGATCATCC TGTTCCATTT GTCTCAAAGA 
AGTACATCCT GTGGTATTTC AACCCACATA GAAATAGCAC ACAGAGCACT GGAATTTTTC 
AGCCAACAAG ATGGCGCTAT TAACTATAAA AAGCTGTTAT TAGAACATCA GGATGCTTAT 
CAGGCTGGGA GTGTGTTTCC TGATGCTTTT TATCCTGACA TCTGCAAAGG AGGAATTTTT 
CACGATGTAT CTGAAGATAC TCACTGGACT CCATTTCTCA ATGCAAGCAT CCATTACATC 
CGACAAAATT ATCCTCTTCC TTGGGAGAAG GATACTGAGA AATTGGTGGC TTTCCTGTTT 
GGCATTATGT CACACATGGT GGCAGATGTG AGCTGGCATA GCCTGGGCAT TGAACAAGGA 
TTCTTGCAGA CAATGGGAGA AATTGATTTT CATGGCTCTT ACCAAGATGC TCATTCTGTT 
GGTGATTTTG GAGGAGATGT GTTGAGCCAT TTTGAACTTA ATTTAAATTA CCTCGGGCGG 
AAATGGTATA TACCTGTCAA AGACCTAATG GGAATCTACA AGCAGCTCTA TGGCAAGGAA 
GTCATAACGA AAAAAGCAAT AATTGACTGT TCATATCTTC AGTTCTTAGA ATTGTATGGT 
GAGATATTGG TTCTTTCCAA GCTTTACCCA AATTTTGCAA AAAAGTCCCC ATTTTTGGTG 
GAACAATTAC AAGATTATTT CCTTGGTGGA CTAGATGACA TGGCATTCTG GTCCACTAAT 
ATGTTTCATC TAACAAGCTA CATGTTACAG AATGGGACCA GTGACTGTTC CCTACCTGAG 
AATCCTCTGT TCATGGCATG TGGTCCCAAA CATAATGGCA GCCTTGGAAT AAAACTGCAG 
AAAAATAAAT GTCATGCAAA TGTAAATGTA TCCTTAGCTG AAAATAGTAA AGGAAATATA 
AATTACACAG AAAGAGGAGT CTTCTACAGT GTGGATTCGT GGGCCCAAGA TTCGGTCTCC 
TTTTTGTATT TGACTTTCAC CAAGAAGTTA AGGAAAATGT TTGCAAGTGA ACAGCCTCAC 
CAGCATAATT ATGTGTCCAG TCCCATAGCA TCTTACTTTC TGTCACTTCC CTATGTGAAG 
CTTGGATGGT CAATGACTTC AGCAGACCTC AACCAGGATG GCCATGAGGA TCTGGTGGTA 
GGAGCACCAG GTTATAGCAG ATCAGGCCAT ATTCAGCCCT CAGGTCGTTT TGGGTCTTCC 
GTGGCAGTAT TGGATTTCAA CAAGGATGGG CTGCCTGACC TAGCAGTGGG AGCCCCCTCG 
GTGGGAGCTG AGAAACTCAC TTACAATGGA GCAGTGTATG TCTTTTTTGG TACCAGAAAA 
GGAAAAATAT CTTCTAAACC AAATGTCACC ATTTTGTGCA AGGACACATA TTGTAACCTT 
GGCTGGACGC TTTTGGCAGC AGATATTAAT GGAGATGGTG CTCCTGACCT GGTGGTTGGG 
TCACCCTATG CACCCAGTGG AGGGAAGCAA AGGGGGATTG TGGCTGCATT CTATTCGGAT 
TCCAAACAAA ATGTCAAAGA TCAACTGAAT GTTGAGGAAG CTAATTGGAT GATAAAGGGG 
AAAGATGACT ATGGCTGGTT TGGATATTCA ATTCATAGTC TCAGCTTAAA GAACAGAACC 
TTGTTATTAG TTGGGAGTCC AACCTGGAAG AATGTGAGTG GGTTAGACAG CGTGTTCTAC 
ATCCGTGATG AGAAACAAAG CATTGGAAAG GTCTATGGCT ATTACCCACC GAGTAGACAA 
AGTTGGTTTA CAATTTCTGG AGATGAGGCA ATGGGAAAAC TGGGCACCTC ACTCTCAAGT 
GGCGTTGTGA AAGTCAATGG AACCCTAAGA CAAGTGCTGC TGGTTGGTGC CCCAACTCGT 
GACGACGTGT CCAAAATGGC ATTCTTATCC ATGACCCTGC ACCAGGGAGG CAGCACAAGG 
GTATATGACT TGACAAATGA GACAGGGCCC TCTCTGCTTA GCACCTTCAG TGGGGACAGG 
CATTTCTCTC GCTTTGGAGG ACTGGTGCAC TTAAGTGACC TGGACAGTGA TGGATTAGAT 
GAGATCATCA TATCAGCCCC TCTGAGAACA GGGGACTTAA CAGCTGGGCT TCTCGGTGGG 
GAGGATGGCC GAGTTTACAT CTACAATGGC AAGGAAGCTC CCCTTGGAGA TGTCACAAGT 
AAATGCAAAT CCTGGATCTC TCCTTGTCCT GAAGAAAAGG CCCAGCATGT ACTGATTTCT 
CTAGAAGAAA ATTCAAGATT CGGGAGCTCT CTGGTCACAG TGAAGTCAAA GACAAAGAAT 
CAAGTTGTTG TTGCTGCTGG GAGGAGCTCT ATGGGAGCCA GACTGGCTGG GACGCTTCAC 
ATCTACAATC TGGGAGTTGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023352085.1
CDS	68..2491
Translation	MSAFRSWFLVLIILFHLSQRSTSCGISTHIEIAHRALEFFSQQDGAINYKKLLLEHQDAYQAGSVFPDAFYPDICKGGIFHDVSEDTHWTPFLNASIHYIRQNYPLPWEKDTEKLVAFLFGIMSHMVADVSWHSLGIEQGFLQTMGEIDFHGSYQDAHSVGDFGGDVLSHFELNLNYLGRKWYIPVKDLMGIYKQLYGKEVITKKAIIDCSYLQFLELYGEILVLSKLYPNFAKKSPFLVEQLQDYFLGGLDDMAFWSTNMFHLTSYMLQNGTSDCSLPENPLFMACGPKHNGSLGIKLQKNKCHANVNVSLAENSKGNINYTERGVFYSVDSWAQDSVSFLYLTFTKKLRKMFASEQPHQHNYVSSPIASYFLSLPYVKLGWSMTSADLNQDGHEDLVVGAPGYSRSGHIQPSGRFGSSVAVLDFNKDGLPDLAVGAPSVGAEKLTYNGAVYVFFGTRKGKISSKPNVTILCKDTYCNLGWTLLAADINGDGAPDLVVGSPYAPSGGKQRGIVAAFYSDSKQNVKDQLNVEEANWMIKGKDDYGWFGYSIHSLSLKNRTLLLVGSPTWKNVSGLDSVFYIRDEKQSIGKVYGYYPPSRQSWFTISGDEAMGKLGTSLSSGVVKVNGTLRQVLLVGAPTRDDVSKMAFLSMTLHQGGSTRVYDLTNETGPSLLSTFSGDRHFSRFGGLVHLSDLDSDGLDEIIISAPLRTGDLTAGLLGGEDGRVYIYNGKEAPLGDVTSKCKSWISPCPEEKAQHVLISLEENSRFGSSLVTVKSKTKNQVVVAAGRSSMGARLAGTLHIYNLGVD

Target ORF information:

RefSeq Version	XM_023496317.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023496317.1

ORF Insert Sequence:

ATGTCTGCTT TCAGGAGCTG GTTTCTTGTG CTGATCATCC TGTTCCATTT GTCTCAAAGA 
AGTACATCCT GTGGTATTTC AACCCACATA GAAATAGCAC ACAGAGCACT GGAATTTTTC 
AGCCAACAAG ATGGCGCTAT TAACTATAAA AAGCTGTTAT TAGAACATCA GGATGCTTAT 
CAGGCTGGGA GTGTGTTTCC TGATGCTTTT TATCCTGACA TCTGCAAAGG AGGAATTTTT 
CACGATGTAT CTGAAGATAC TCACTGGACT CCATTTCTCA ATGCAAGCAT CCATTACATC 
CGACAAAATT ATCCTCTTCC TTGGGAGAAG GATACTGAGA AATTGGTGGC TTTCCTGTTT 
GGCATTATGT CACACATGGT GGCAGATGTG AGCTGGCATA GCCTGGGCAT TGAACAAGGA 
TTCTTGCAGA CAATGGGAGA AATTGATTTT CATGGCTCTT ACCAAGATGC TCATTCTGTT 
GGTGATTTTG GAGGAGATGT GTTGAGCCAT TTTGAACTTA ATTTAAATTA CCTCGGGCGG 
AAATGGTATA TACCTGTCAA AGACCTAATG GGAATCTACA AGCAGCTCTA TGGCAAGGAA 
GTCATAACGA AAAAAGCAAT AATTGACTGT TCATATCTTC AGTTCTTAGA ATTGTATGGT 
GAGATATTGG TTCTTTCCAA GCTTTACCCA AATTTTGCAA AAAAGTCCCC ATTTTTGGTG 
GAACAATTAC AAGATTATTT CCTTGGTGGA CTAGATGACA TGGCATTCTG GTCCACTAAT 
ATGTTTCATC TAACAAGCTA CATGTTACAG AATGGGACCA GTGACTGTTC CCTACCTGAG 
AATCCTCTGT TCATGGCATG TGGTCCCAAA CATAATGGCA GCCTTGGAAT AAAACTGCAG 
AAAAATAAAT GTCATGCAAA TGTAAATGTA TCCTTAGCTG AAAATAGTAA AGGAAATATA 
AATTACACAG AAAGAGGAGT CTTCTACAGT GTGGATTCGT GGGCCCAAGA TTCGGTCTCC 
TTTTTGTATT TGACTTTCAC CAAGAAGTTA AGGAAAATGT TTGCAAGTGA ACAGCCTCAC 
CAGCATAATT ATGTGTCCAG TCCCATAGCA TCTTACTTTC TGTCACTTCC CTATGTGAAG 
CTTGGATGGT CAATGACTTC AGCAGACCTC AACCAGGATG GCCATGAGGA TCTGGTGGTA 
GGAGCACCAG GTTATAGCAG ATCAGGCCAT ATTCAGCCCT CAGGTCGTTT TGGGTCTTCC 
GTGGCAGTAT TGGATTTCAA CAAGGATGGG CTGCCTGACC TAGCAGTGGG AGCCCCCTCG 
GTGGGAGCTG AGAAACTCAC TTACAATGGA GCAGTGTATG TCTTTTTTGG TACCAGAAAA 
GGAAAAATAT CTTCTAAACC AAATGTCACC ATTTTGTGCA AGGACACATA TTGTAACCTT 
GGCTGGACGC TTTTGGCAGC AGATATTAAT GGAGATGGTG CTCCTGACCT GGTGGTTGGG 
TCACCCTATG CACCCAGTGG AGGGAAGCAA AGGGGGATTG TGGCTGCATT CTATTCGGAT 
TCCAAACAAA ATGTCAAAGA TCAACTGAAT GTTGAGGAAG CTAATTGGAT GATAAAGGGG 
AAAGATGACT ATGGCTGGTT TGGATATTCA ATTCATAGTC TCAGCTTAAA GAACAGAACC 
TTGTTATTAG TTGGGAGTCC AACCTGGAAG AATGTGAGTG GGTTAGACAG CGTGTTCTAC 
ATCCGTGATG AGAAACAAAG CATTGGAAAG GTCTATGGCT ATTACCCACC GAGTAGACAA 
AGTTGGTTTA CAATTTCTGG AGATGAGGCA ATGGGAAAAC TGGGCACCTC ACTCTCAAGT 
GGCGTTGTGA AAGTCAATGG AACCCTAAGA CAAGTGCTGC TGGTTGGTGC CCCAACTCGT 
GACGACGTGT CCAAAATGGC ATTCTTATCC ATGACCCTGC ACCAGGGAGG CAGCACAAGG 
GTATATGACT TGACAAATGA GACAGGGCCC TCTCTGCTTA GCACCTTCAG TGGGGACAGG 
CATTTCTCTC GCTTTGGAGG ACTGGTGCAC TTAAGTGACC TGGACAGTGA TGGATTAGAT 
GAGATCATCA TATCAGCCCC TCTGAGAACA GGGGACTTAA CAGCTGGGCT TCTCGGTGGG 
GAGGATGGCC GAGTTTACAT CTACAATGGC AAGGAAGCTC CCCTTGGAGA TGTCACAAGT 
AAATGCAAAT CCTGGATCTC TCCTTGTCCT GAAGAAAAGG CCCAGCATGT ACTGATTTCT 
CTAGAAGAAA ATTCAAGATT CGGGAGCTCT CTGGTCACAG TGAAGTCAAA GACAAAGAAT 
CAAGTTGTTG TTGCTGCTGG GAGGAGCTCT ATGGGAGCCA GACTGGCTGG GACGCTTCAC 
ATCTACAATC TGGGAGTTGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPLD1 ( XM_031946779.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031802639.1 Sarcophilus harrisii glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa221519
Clone ID Related Accession (Same CDS sequence)	XM_031946779.1
Accession Version	XM_031946779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	phosphatidylinositol-glycan-specific phospholipase D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGGC AGAGGCAGGC GGAGCAGCAG AGCTCGGGCC GCGCGGCGCA ACCCCAGAGC 
TGGTTTCTTG TGCTGATCAT CCTGTTCCAT TTGTCTCAAA GAAGTACATC CTGTGGTATT 
TCAACCCACA TAGAAATAGC ACACAGAGCA CTGGAATTTT TCAGCCAACA AGATGGCGCT 
ATTAACTATA AAAAGCTGTT ATTAGAACAT CAGGATGCTT ATCAGGCTGG GAGTGTGTTT 
CCTGATGCTT TTTATCCTGA CATCTGCAAA GGAGGAATTT TTCATGATGT ATCTGAAGAT 
ACTCACTGGA CTCCATTTCT CAATGCAAGC ATCCATTACA TCCGACAAAA TTATCCTCTT 
CCTTGGGAGA AGGATACTGA GAAATTGGTG GCTTTCCTGT TTGGCATTAT GTCACACATG 
GTGGCAGATG TGAGCTGGCA TAGCCTGGGC ATTGAACAAG GATTCTTGCA GACAATGGGA 
GAAATTGATT TTCATGGCTC TTACCAAGAT GCTCATTCTG TTGGTGATTT TGGAGGAGAT 
GTGTTGAGCC ATTTTGAACT TAATTTAAAT TACCTCGGGC GGAAATGGTA TATACCTGTC 
AAAGACCTAA TGGGAATCTA CAAGCAGCTC TATGGCAAGG AAGTCATAAC GAAAAAAGCA 
ATAATTGACT GTTCATATCT TCAGTTCTTA GAATTGTATG GTGAGATATT GGTTCTTTCC 
AAGCTTTACC CAAATTTTGC AAAAAAGTCC CCATTTTTGG TGGAACAATT ACAAGATTAT 
TTCCTTGGTG GACTAGATGA CATGGCATTC TGGTCCACTA ATATGTTTCA TCTAACAAGC 
TACATGTTAC AGAATGGGAC CAGTGACTGT TCCCTACCTG AGAATCCTCT GTTCATGGCA 
TGTGGTCCCA AACATAATGG CAGCCTTGGA ATAAAACTGC AGAAAAATAA ATGTCATGCA 
AATGTAAATG TATCCTTAGC TGAAAATAGT AAAGGAAATA TAAATTACAC AGAAAGAGGA 
GTCTTCTACA GTGTGGATTC GTGGGCCCAA GATTCGGTCT CCTTTTTGTA TTTGACTTTC 
ACCAAGAAGT TAAGGAAAAT GTTTGCAAGT GAACAGCCTC ACCAGCATAA TTATGTGTCC 
AGTCCCATAG CATCTTACTT TCTGTCACTT CCCTATGTGA AGCTTGGATG GTCAATGACT 
TCAGCAGACC TCAACCAGGA TGGCCATGAG GATCTGGTGG TAGGAGCACC AGGTTATAGC 
AGATCAGGCC ATATTCAGGT GGGACGAGTA TATCTCATCT ACAGCAATGC TATAGGTTTG 
CCTCCCGTAG ACTTGGATTT GGACAAAGAA GCACATGTAA TTCTAGAGGG CTTTCAGCCC 
TCAGGTCGTT TTGGGTCTTC CGTGGCAGTA TTGGATTTCA ACAAGGATGG GCTGCCTGAC 
CTAGCAGTGG GAGCCCCCTC GGTGGGAGCT GAGAAACTCA CTTACAATGG AGCAGTGTAT 
GTCTTTTTTG GTACCAGAAA AGGAAAAATA TCTTCTAAAC CAAATGTCAC CATTTTGTGC 
AAGGACACAT ATTGTAACCT TGGCTGGACG CTTTTGGCAG CAGATATTAA TGGAGATGGT 
GCTCCTGACC TGGTGGTTGG GTCACCCTAT GCACCCAGTG GAGGGAAGCA AAGGGGGATT 
GTGGCTGCAT TCTATTCGGA TTCCAAACAA AATGTCAAAG ATCAACTGAA TGTTGAGGAA 
GCTAATTGGA TGATAAAGGG GAAAGATGAC TATGGCTGGT TTGGATATTC AATTCATAGT 
CTCAGCTTAA AGAACAGAAC CTTGTTATTA GTTGGGAGTC CAACCTGGAA GAATGTGAGT 
GGGTTAGACA GCGTGTTCTA CATCCGTGAT GAGAAACAAA GCATTGGAAA GGTCTATGGC 
TATTACCCAC CGAGTAGACA AAGTTGGTTT ACAATTTCTG GAGATGAGGC AATGGGAAAA 
CTGGGCACCT CACTCTCAAG TGGCGTTGTG AAAGTCAATG GAACCCTAAG ACAAGTGCTG 
CTGGTTGGTG CCCCAACTCG TGACGACGTG TCCAAAATGG CATTCTTATC CATGACCCTG 
CACCAGGGAG GCAGCACAAG GGTATATGAC TTGACAAATG AGACAGGGCC CTCTCTGCTT 
AGCACCTTCA GTGGGGACAG GCATTTCTCT CGCTTTGGAG GACTGGTGCA CTTAAGTGAC 
CTGGACAGTG ATGGATTAGA TGAGATCATC ATATCAGCCC CTCTGAGAAC AGGGGACTTA 
ACAGCTGGGC TTCTCGGTGG GGAGGATGGC CGAGTTTACA TCTACAATGG CAAGGAAGCT 
CCCCTTGGAG ATGTCACAAG TAAATGCAAA TCCTGGATCT CTCCTTGTCC TGAAGAAAAG 
GCCCAGCATG TACTGATTTC TCTAGAAGAA AATTCAAGAT TCGGGAGCTC TCTGGTCACA 
GTGAAGTCAA AGACAAAGAA TCAAGTTGTT GTTGCTGCTG GGAGGAGCTC TATGGGAGCC 
AGACTGGCTG GGACGCTTCA CATCTACAAT CTGGGAGTTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031802639.1
CDS	2..2566
Translation	MRRQRQAEQQSSGRAAQPQSWFLVLIILFHLSQRSTSCGISTHIEIAHRALEFFSQQDGAINYKKLLLEHQDAYQAGSVFPDAFYPDICKGGIFHDVSEDTHWTPFLNASIHYIRQNYPLPWEKDTEKLVAFLFGIMSHMVADVSWHSLGIEQGFLQTMGEIDFHGSYQDAHSVGDFGGDVLSHFELNLNYLGRKWYIPVKDLMGIYKQLYGKEVITKKAIIDCSYLQFLELYGEILVLSKLYPNFAKKSPFLVEQLQDYFLGGLDDMAFWSTNMFHLTSYMLQNGTSDCSLPENPLFMACGPKHNGSLGIKLQKNKCHANVNVSLAENSKGNINYTERGVFYSVDSWAQDSVSFLYLTFTKKLRKMFASEQPHQHNYVSSPIASYFLSLPYVKLGWSMTSADLNQDGHEDLVVGAPGYSRSGHIQVGRVYLIYSNAIGLPPVDLDLDKEAHVILEGFQPSGRFGSSVAVLDFNKDGLPDLAVGAPSVGAEKLTYNGAVYVFFGTRKGKISSKPNVTILCKDTYCNLGWTLLAADINGDGAPDLVVGSPYAPSGGKQRGIVAAFYSDSKQNVKDQLNVEEANWMIKGKDDYGWFGYSIHSLSLKNRTLLLVGSPTWKNVSGLDSVFYIRDEKQSIGKVYGYYPPSRQSWFTISGDEAMGKLGTSLSSGVVKVNGTLRQVLLVGAPTRDDVSKMAFLSMTLHQGGSTRVYDLTNETGPSLLSTFSGDRHFSRFGGLVHLSDLDSDGLDEIIISAPLRTGDLTAGLLGGEDGRVYIYNGKEAPLGDVTSKCKSWISPCPEEKAQHVLISLEENSRFGSSLVTVKSKTKNQVVVAAGRSSMGARLAGTLHIYNLGVD

Target ORF information:

RefSeq Version	XM_031946779.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031946779.1

ORF Insert Sequence:

ATGCGCAGGC AGAGGCAGGC GGAGCAGCAG AGCTCGGGCC GCGCGGCGCA ACCCCAGAGC 
TGGTTTCTTG TGCTGATCAT CCTGTTCCAT TTGTCTCAAA GAAGTACATC CTGTGGTATT 
TCAACCCACA TAGAAATAGC ACACAGAGCA CTGGAATTTT TCAGCCAACA AGATGGCGCT 
ATTAACTATA AAAAGCTGTT ATTAGAACAT CAGGATGCTT ATCAGGCTGG GAGTGTGTTT 
CCTGATGCTT TTTATCCTGA CATCTGCAAA GGAGGAATTT TTCATGATGT ATCTGAAGAT 
ACTCACTGGA CTCCATTTCT CAATGCAAGC ATCCATTACA TCCGACAAAA TTATCCTCTT 
CCTTGGGAGA AGGATACTGA GAAATTGGTG GCTTTCCTGT TTGGCATTAT GTCACACATG 
GTGGCAGATG TGAGCTGGCA TAGCCTGGGC ATTGAACAAG GATTCTTGCA GACAATGGGA 
GAAATTGATT TTCATGGCTC TTACCAAGAT GCTCATTCTG TTGGTGATTT TGGAGGAGAT 
GTGTTGAGCC ATTTTGAACT TAATTTAAAT TACCTCGGGC GGAAATGGTA TATACCTGTC 
AAAGACCTAA TGGGAATCTA CAAGCAGCTC TATGGCAAGG AAGTCATAAC GAAAAAAGCA 
ATAATTGACT GTTCATATCT TCAGTTCTTA GAATTGTATG GTGAGATATT GGTTCTTTCC 
AAGCTTTACC CAAATTTTGC AAAAAAGTCC CCATTTTTGG TGGAACAATT ACAAGATTAT 
TTCCTTGGTG GACTAGATGA CATGGCATTC TGGTCCACTA ATATGTTTCA TCTAACAAGC 
TACATGTTAC AGAATGGGAC CAGTGACTGT TCCCTACCTG AGAATCCTCT GTTCATGGCA 
TGTGGTCCCA AACATAATGG CAGCCTTGGA ATAAAACTGC AGAAAAATAA ATGTCATGCA 
AATGTAAATG TATCCTTAGC TGAAAATAGT AAAGGAAATA TAAATTACAC AGAAAGAGGA 
GTCTTCTACA GTGTGGATTC GTGGGCCCAA GATTCGGTCT CCTTTTTGTA TTTGACTTTC 
ACCAAGAAGT TAAGGAAAAT GTTTGCAAGT GAACAGCCTC ACCAGCATAA TTATGTGTCC 
AGTCCCATAG CATCTTACTT TCTGTCACTT CCCTATGTGA AGCTTGGATG GTCAATGACT 
TCAGCAGACC TCAACCAGGA TGGCCATGAG GATCTGGTGG TAGGAGCACC AGGTTATAGC 
AGATCAGGCC ATATTCAGGT GGGACGAGTA TATCTCATCT ACAGCAATGC TATAGGTTTG 
CCTCCCGTAG ACTTGGATTT GGACAAAGAA GCACATGTAA TTCTAGAGGG CTTTCAGCCC 
TCAGGTCGTT TTGGGTCTTC CGTGGCAGTA TTGGATTTCA ACAAGGATGG GCTGCCTGAC 
CTAGCAGTGG GAGCCCCCTC GGTGGGAGCT GAGAAACTCA CTTACAATGG AGCAGTGTAT 
GTCTTTTTTG GTACCAGAAA AGGAAAAATA TCTTCTAAAC CAAATGTCAC CATTTTGTGC 
AAGGACACAT ATTGTAACCT TGGCTGGACG CTTTTGGCAG CAGATATTAA TGGAGATGGT 
GCTCCTGACC TGGTGGTTGG GTCACCCTAT GCACCCAGTG GAGGGAAGCA AAGGGGGATT 
GTGGCTGCAT TCTATTCGGA TTCCAAACAA AATGTCAAAG ATCAACTGAA TGTTGAGGAA 
GCTAATTGGA TGATAAAGGG GAAAGATGAC TATGGCTGGT TTGGATATTC AATTCATAGT 
CTCAGCTTAA AGAACAGAAC CTTGTTATTA GTTGGGAGTC CAACCTGGAA GAATGTGAGT 
GGGTTAGACA GCGTGTTCTA CATCCGTGAT GAGAAACAAA GCATTGGAAA GGTCTATGGC 
TATTACCCAC CGAGTAGACA AAGTTGGTTT ACAATTTCTG GAGATGAGGC AATGGGAAAA 
CTGGGCACCT CACTCTCAAG TGGCGTTGTG AAAGTCAATG GAACCCTAAG ACAAGTGCTG 
CTGGTTGGTG CCCCAACTCG TGACGACGTG TCCAAAATGG CATTCTTATC CATGACCCTG 
CACCAGGGAG GCAGCACAAG GGTATATGAC TTGACAAATG AGACAGGGCC CTCTCTGCTT 
AGCACCTTCA GTGGGGACAG GCATTTCTCT CGCTTTGGAG GACTGGTGCA CTTAAGTGAC 
CTGGACAGTG ATGGATTAGA TGAGATCATC ATATCAGCCC CTCTGAGAAC AGGGGACTTA 
ACAGCTGGGC TTCTCGGTGG GGAGGATGGC CGAGTTTACA TCTACAATGG CAAGGAAGCT 
CCCCTTGGAG ATGTCACAAG TAAATGCAAA TCCTGGATCT CTCCTTGTCC TGAAGAAAAG 
GCCCAGCATG TACTGATTTC TCTAGAAGAA AATTCAAGAT TCGGGAGCTC TCTGGTCACA 
GTGAAGTCAA AGACAAAGAA TCAAGTTGTT GTTGCTGCTG GGAGGAGCTC TATGGGAGCC 
AGACTGGCTG GGACGCTTCA CATCTACAAT CTGGGAGTTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPLD1 ( XM_003760134.4 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003760182.1 Sarcophilus harrisii glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X2, mRNA.

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CloneID	OSa221520
Clone ID Related Accession (Same CDS sequence)	XM_003760134.4
Accession Version	XM_003760134.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	phosphatidylinositol-glycan-specific phospholipase D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_003760134.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTT TCAGGAGCTG GTTTCTTGTG CTGATCATCC TGTTCCATTT GTCTCAAAGA 
AGTACATCCT GTGGTATTTC AACCCACATA GAAATAGCAC ACAGAGCACT GGAATTTTTC 
AGCCAACAAG ATGGCGCTAT TAACTATAAA AAGCTGTTAT TAGAACATCA GGATGCTTAT 
CAGGCTGGGA GTGTGTTTCC TGATGCTTTT TATCCTGACA TCTGCAAAGG AGGAATTTTT 
CATGATGTAT CTGAAGATAC TCACTGGACT CCATTTCTCA ATGCAAGCAT CCATTACATC 
CGACAAAATT ATCCTCTTCC TTGGGAGAAG GATACTGAGA AATTGGTGGC TTTCCTGTTT 
GGCATTATGT CACACATGGT GGCAGATGTG AGCTGGCATA GCCTGGGCAT TGAACAAGGA 
TTCTTGCAGA CAATGGGAGA AATTGATTTT CATGGCTCTT ACCAAGATGC TCATTCTGTT 
GGTGATTTTG GAGGAGATGT GTTGAGCCAT TTTGAACTTA ATTTAAATTA CCTCGGGCGG 
AAATGGTATA TACCTGTCAA AGACCTAATG GGAATCTACA AGCAGCTCTA TGGCAAGGAA 
GTCATAACGA AAAAAGCAAT AATTGACTGT TCATATCTTC AGTTCTTAGA ATTGTATGGT 
GAGATATTGG TTCTTTCCAA GCTTTACCCA AATTTTGCAA AAAAGTCCCC ATTTTTGGTG 
GAACAATTAC AAGATTATTT CCTTGGTGGA CTAGATGACA TGGCATTCTG GTCCACTAAT 
ATGTTTCATC TAACAAGCTA CATGTTACAG AATGGGACCA GTGACTGTTC CCTACCTGAG 
AATCCTCTGT TCATGGCATG TGGTCCCAAA CATAATGGCA GCCTTGGAAT AAAACTGCAG 
AAAAATAAAT GTCATGCAAA TGTAAATGTA TCCTTAGCTG AAAATAGTAA AGGAAATATA 
AATTACACAG AAAGAGGAGT CTTCTACAGT GTGGATTCGT GGGCCCAAGA TTCGGTCTCC 
TTTTTGTATT TGACTTTCAC CAAGAAGTTA AGGAAAATGT TTGCAAGTGA ACAGCCTCAC 
CAGCATAATT ATGTGTCCAG TCCCATAGCA TCTTACTTTC TGTCACTTCC CTATGTGAAG 
CTTGGATGGT CAATGACTTC AGCAGACCTC AACCAGGATG GCCATGAGGA TCTGGTGGTA 
GGAGCACCAG GTTATAGCAG ATCAGGCCAT ATTCAGGTGG GACGAGTATA TCTCATCTAC 
AGCAATGCTA TAGGTTTGCC TCCCGTAGAC TTGGATTTGG ACAAAGAAGC ACATGTAATT 
CTAGAGGGCT TTCAGCCCTC AGGTCGTTTT GGGTCTTCCG TGGCAGTATT GGATTTCAAC 
AAGGATGGGC TGCCTGACCT AGCAGTGGGA GCCCCCTCGG TGGGAGCTGA GAAACTCACT 
TACAATGGAG CAGTGTATGT CTTTTTTGGT ACCAGAAAAG GAAAAATATC TTCTAAACCA 
AATGTCACCA TTTTGTGCAA GGACACATAT TGTAACCTTG GCTGGACGCT TTTGGCAGCA 
GATATTAATG GAGATGGTGC TCCTGACCTG GTGGTTGGGT CACCCTATGC ACCCAGTGGA 
GGGAAGCAAA GGGGGATTGT GGCTGCATTC TATTCGGATT CCAAACAAAA TGTCAAAGAT 
CAACTGAATG TTGAGGAAGC TAATTGGATG ATAAAGGGGA AAGATGACTA TGGCTGGTTT 
GGATATTCAA TTCATAGTCT CAGCTTAAAG AACAGAACCT TGTTATTAGT TGGGAGTCCA 
ACCTGGAAGA ATGTGAGTGG GTTAGACAGC GTGTTCTACA TCCGTGATGA GAAACAAAGC 
ATTGGAAAGG TCTATGGCTA TTACCCACCG AGTAGACAAA GTTGGTTTAC AATTTCTGGA 
GATGAGGCAA TGGGAAAACT GGGCACCTCA CTCTCAAGTG GCGTTGTGAA AGTCAATGGA 
ACCCTAAGAC AAGTGCTGCT GGTTGGTGCC CCAACTCGTG ACGACGTGTC CAAAATGGCA 
TTCTTATCCA TGACCCTGCA CCAGGGAGGC AGCACAAGGG TATATGACTT GACAAATGAG 
ACAGGGCCCT CTCTGCTTAG CACCTTCAGT GGGGACAGGC ATTTCTCTCG CTTTGGAGGA 
CTGGTGCACT TAAGTGACCT GGACAGTGAT GGATTAGATG AGATCATCAT ATCAGCCCCT 
CTGAGAACAG GGGACTTAAC AGCTGGGCTT CTCGGTGGGG AGGATGGCCG AGTTTACATC 
TACAATGGCA AGGAAGCTCC CCTTGGAGAT GTCACAAGTA AATGCAAATC CTGGATCTCT 
CCTTGTCCTG AAGAAAAGGC CCAGCATGTA CTGATTTCTC TAGAAGAAAA TTCAAGATTC 
GGGAGCTCTC TGGTCACAGT GAAGTCAAAG ACAAAGAATC AAGTTGTTGT TGCTGCTGGG 
AGGAGCTCTA TGGGAGCCAG ACTGGCTGGG ACGCTTCACA TCTACAATCT GGGAGTTGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003760182.1
CDS	58..2580
Translation	MSAFRSWFLVLIILFHLSQRSTSCGISTHIEIAHRALEFFSQQDGAINYKKLLLEHQDAYQAGSVFPDAFYPDICKGGIFHDVSEDTHWTPFLNASIHYIRQNYPLPWEKDTEKLVAFLFGIMSHMVADVSWHSLGIEQGFLQTMGEIDFHGSYQDAHSVGDFGGDVLSHFELNLNYLGRKWYIPVKDLMGIYKQLYGKEVITKKAIIDCSYLQFLELYGEILVLSKLYPNFAKKSPFLVEQLQDYFLGGLDDMAFWSTNMFHLTSYMLQNGTSDCSLPENPLFMACGPKHNGSLGIKLQKNKCHANVNVSLAENSKGNINYTERGVFYSVDSWAQDSVSFLYLTFTKKLRKMFASEQPHQHNYVSSPIASYFLSLPYVKLGWSMTSADLNQDGHEDLVVGAPGYSRSGHIQVGRVYLIYSNAIGLPPVDLDLDKEAHVILEGFQPSGRFGSSVAVLDFNKDGLPDLAVGAPSVGAEKLTYNGAVYVFFGTRKGKISSKPNVTILCKDTYCNLGWTLLAADINGDGAPDLVVGSPYAPSGGKQRGIVAAFYSDSKQNVKDQLNVEEANWMIKGKDDYGWFGYSIHSLSLKNRTLLLVGSPTWKNVSGLDSVFYIRDEKQSIGKVYGYYPPSRQSWFTISGDEAMGKLGTSLSSGVVKVNGTLRQVLLVGAPTRDDVSKMAFLSMTLHQGGSTRVYDLTNETGPSLLSTFSGDRHFSRFGGLVHLSDLDSDGLDEIIISAPLRTGDLTAGLLGGEDGRVYIYNGKEAPLGDVTSKCKSWISPCPEEKAQHVLISLEENSRFGSSLVTVKSKTKNQVVVAAGRSSMGARLAGTLHIYNLGVD

Target ORF information:

RefSeq Version	XM_003760134.4
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003760134.4

ORF Insert Sequence:

ATGTCTGCTT TCAGGAGCTG GTTTCTTGTG CTGATCATCC TGTTCCATTT GTCTCAAAGA 
AGTACATCCT GTGGTATTTC AACCCACATA GAAATAGCAC ACAGAGCACT GGAATTTTTC 
AGCCAACAAG ATGGCGCTAT TAACTATAAA AAGCTGTTAT TAGAACATCA GGATGCTTAT 
CAGGCTGGGA GTGTGTTTCC TGATGCTTTT TATCCTGACA TCTGCAAAGG AGGAATTTTT 
CATGATGTAT CTGAAGATAC TCACTGGACT CCATTTCTCA ATGCAAGCAT CCATTACATC 
CGACAAAATT ATCCTCTTCC TTGGGAGAAG GATACTGAGA AATTGGTGGC TTTCCTGTTT 
GGCATTATGT CACACATGGT GGCAGATGTG AGCTGGCATA GCCTGGGCAT TGAACAAGGA 
TTCTTGCAGA CAATGGGAGA AATTGATTTT CATGGCTCTT ACCAAGATGC TCATTCTGTT 
GGTGATTTTG GAGGAGATGT GTTGAGCCAT TTTGAACTTA ATTTAAATTA CCTCGGGCGG 
AAATGGTATA TACCTGTCAA AGACCTAATG GGAATCTACA AGCAGCTCTA TGGCAAGGAA 
GTCATAACGA AAAAAGCAAT AATTGACTGT TCATATCTTC AGTTCTTAGA ATTGTATGGT 
GAGATATTGG TTCTTTCCAA GCTTTACCCA AATTTTGCAA AAAAGTCCCC ATTTTTGGTG 
GAACAATTAC AAGATTATTT CCTTGGTGGA CTAGATGACA TGGCATTCTG GTCCACTAAT 
ATGTTTCATC TAACAAGCTA CATGTTACAG AATGGGACCA GTGACTGTTC CCTACCTGAG 
AATCCTCTGT TCATGGCATG TGGTCCCAAA CATAATGGCA GCCTTGGAAT AAAACTGCAG 
AAAAATAAAT GTCATGCAAA TGTAAATGTA TCCTTAGCTG AAAATAGTAA AGGAAATATA 
AATTACACAG AAAGAGGAGT CTTCTACAGT GTGGATTCGT GGGCCCAAGA TTCGGTCTCC 
TTTTTGTATT TGACTTTCAC CAAGAAGTTA AGGAAAATGT TTGCAAGTGA ACAGCCTCAC 
CAGCATAATT ATGTGTCCAG TCCCATAGCA TCTTACTTTC TGTCACTTCC CTATGTGAAG 
CTTGGATGGT CAATGACTTC AGCAGACCTC AACCAGGATG GCCATGAGGA TCTGGTGGTA 
GGAGCACCAG GTTATAGCAG ATCAGGCCAT ATTCAGGTGG GACGAGTATA TCTCATCTAC 
AGCAATGCTA TAGGTTTGCC TCCCGTAGAC TTGGATTTGG ACAAAGAAGC ACATGTAATT 
CTAGAGGGCT TTCAGCCCTC AGGTCGTTTT GGGTCTTCCG TGGCAGTATT GGATTTCAAC 
AAGGATGGGC TGCCTGACCT AGCAGTGGGA GCCCCCTCGG TGGGAGCTGA GAAACTCACT 
TACAATGGAG CAGTGTATGT CTTTTTTGGT ACCAGAAAAG GAAAAATATC TTCTAAACCA 
AATGTCACCA TTTTGTGCAA GGACACATAT TGTAACCTTG GCTGGACGCT TTTGGCAGCA 
GATATTAATG GAGATGGTGC TCCTGACCTG GTGGTTGGGT CACCCTATGC ACCCAGTGGA 
GGGAAGCAAA GGGGGATTGT GGCTGCATTC TATTCGGATT CCAAACAAAA TGTCAAAGAT 
CAACTGAATG TTGAGGAAGC TAATTGGATG ATAAAGGGGA AAGATGACTA TGGCTGGTTT 
GGATATTCAA TTCATAGTCT CAGCTTAAAG AACAGAACCT TGTTATTAGT TGGGAGTCCA 
ACCTGGAAGA ATGTGAGTGG GTTAGACAGC GTGTTCTACA TCCGTGATGA GAAACAAAGC 
ATTGGAAAGG TCTATGGCTA TTACCCACCG AGTAGACAAA GTTGGTTTAC AATTTCTGGA 
GATGAGGCAA TGGGAAAACT GGGCACCTCA CTCTCAAGTG GCGTTGTGAA AGTCAATGGA 
ACCCTAAGAC AAGTGCTGCT GGTTGGTGCC CCAACTCGTG ACGACGTGTC CAAAATGGCA 
TTCTTATCCA TGACCCTGCA CCAGGGAGGC AGCACAAGGG TATATGACTT GACAAATGAG 
ACAGGGCCCT CTCTGCTTAG CACCTTCAGT GGGGACAGGC ATTTCTCTCG CTTTGGAGGA 
CTGGTGCACT TAAGTGACCT GGACAGTGAT GGATTAGATG AGATCATCAT ATCAGCCCCT 
CTGAGAACAG GGGACTTAAC AGCTGGGCTT CTCGGTGGGG AGGATGGCCG AGTTTACATC 
TACAATGGCA AGGAAGCTCC CCTTGGAGAT GTCACAAGTA AATGCAAATC CTGGATCTCT 
CCTTGTCCTG AAGAAAAGGC CCAGCATGTA CTGATTTCTC TAGAAGAAAA TTCAAGATTC 
GGGAGCTCTC TGGTCACAGT GAAGTCAAAG ACAAAGAATC AAGTTGTTGT TGCTGCTGGG 
AGGAGCTCTA TGGGAGCCAG ACTGGCTGGG ACGCTTCACA TCTACAATCT GGGAGTTGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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GPLD1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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