ALS2 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene
Clones
gRNAs

Summary

Gene Symbol	ALS2
Entrez Gene ID	100088146
Full Name	alsin
Synonyms	ALS2
Gene Type	protein-coding
Organism	Ornithorhynchus anatinus(platypus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029068795.1	XP_028924628.1	alsin isoform X1
XM_029068796.1	XP_028924629.1	alsin isoform X1
XM_016226454.1	XP_016081940.1	alsin
XM_029068797.1	XP_028924630.1	alsin isoform X1
XM_029068798.1	XP_028924631.1	alsin isoform X2

Pathways from BioSystems
Gene Ontology

The following ALS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOc37047	XM_016226454.1 Latest version!	Ornithorhynchus anatinus alsin (LOC100088146), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OOc131912	XM_029068796.1 Latest version!	Ornithorhynchus anatinus alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOc131912	XM_029068795.1 Latest version!	Ornithorhynchus anatinus alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOc131912	XM_029068797.1 Latest version!	Ornithorhynchus anatinus alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOc131913	XM_029068798.1 Latest version!	Ornithorhynchus anatinus alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALS2 ( XM_016226454.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_016081940.1 Ornithorhynchus anatinus alsin (LOC100088146), mRNA.

Price & Availability↑

CloneID	OOc37047
Clone ID Related Accession (Same CDS sequence)	XM_016226454.1
Accession Version	XM_016226454.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1460563200000
Organism	Ornithorhynchus anatinus(platypus)
Product	alsin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001741216.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 19% of CDS bases ##RefSeq-Attributes-END##

ATGCCGCCGT TTTTAATTCT GCTCTACGCT CACTCTTCCA GGCGCCCCAA GAATGGCCCC 
CAGAACCGGG GACCAGGCCC TCATCCTGGG TGCTTCCCAC AGGCGTCTAC GGTCCACGGG 
GACGATGGAG ATGAAAGGTT GATGCTTTTG GGGCCGCCAT CTCTGATTTC ACGGCCCTTG 
AGTGGGAATC TCCCCGAAGG TGACAGAGCA CGGAATGACT ACTCCCCAGC TGAGAACCTG 
GGGAACCCAT CCGCTATTAA GCCTTTACAT GGTTCCTTCA TTACAGCAGC CGGGCGAGAG 
CTGGGAGCGC GGCAGTTTGC CTGGAAGGCC GATGCCGCGA ACAACGTCCA GACACGCGTG 
GTGCGCCGGA GTCTCATGGC AACGTTCTCT CCAGAATGTA TCGCAAGGGC ATGTGTTGAG 
AGAACGCCAA TTTCATATGA CATAACCATA GATCGGCTGT ATCAAGCGCT GGAAAATATT 
CACTCACATG GCCGGTTTGG CGAGGAGCTG GAATCCTCCC ATGACGATAA AATTGGTCAG 
GGCCGTGCAG TACCTTTAGC GGCCGATGCC GCGAACAACG TCCAGACATG CGTGGTGCGC 
CGGAGTGGCA ACGTTCTCTC CAGAATGGAC TTCTTAAATA AAAACCAGGA GCTGTTACAG 
GAGCTGTCGG AGACGAAAGA CGGGGACGTC CAGCTGTTGG AGATGCTGAA TGCCTTATTC 
TTCCTCCCAA TCCGGCGGCT TCATGATTAC GCCCGAGCTC TGCTAAAGCT GGCTACATGT 
TTTGAGGTGG CCTCTCCGGA GTACCAGACG CTGCACGATT CCAGTTCCTG CTACGAGACC 
CTCGCTCTCC ACCTTGGCAG GAAAAGGAAG GAAGCAGAAG CCACGCTGAG CTTCTGGAAG 
ACGTTCCCCG GAAAGATGAC GGACTCCTTG AGGAAGCCAG AGCGCAGGCT AATCTGCGAG 
AGCAGCAATC GAGCGCTGGC CCTGCAGCAT GCTGGGAGAT TCTCCGTGAA TTGGTTCATT 
CTCTTTAACG ATGCCTTGGT CCATGCCCAG TTCTCGACCC ATCACATCTT CCCCCTGTCC 
ACTCTGTGGG TCGAGCCTAT CTCAGAAGAA GGCAACAGCG CGAACGGCCT GAAGTTGACG 
ACGCCGGAGG AACAGTTCAC CCTCATTTCC TCGACCCCGC AGGAGAAGAC CAAGTGGCTG 
CGGGCCCTGA GCCAGGCGGT GGACCAGGCC CTGAGGGGCG CGGCCGACTT CCCCCCCTAC 
GGCAGCGGCG CCCCGCGGCC GGACCCCCCC GCCTCCCGCA GCGCCAGCTA CACCTTCTAC 
AAGGCCTCCC GCCTCAAGGA AGCCACCTAC GAGGGCCGCT GGCTCTCCGG GAAGCCCCAC 
GGCAGAGGGG TCTTGAAGTG GACCGACGGC AAGACGTACT CCGGTATGTT TCGGAATGGC 
CTGGAAGATG GATACGGGGA ATATAAAGTC CCAAACAAAG CACTTAACAA AGAAGACCAC 
TACGTCGGGT ACTGGAAAGA AGGAAAAATG TGCGGACAAG GAGTTTACAG CTACGCCACG 
GGCGAAGTGT ACGAAGGCTA CTTCCAAGAG AACGTCCGCC ACGGCCACGG GCTTCTCCGC 
AGCGGCAAGC TGACCTCCAC CTCCCCCAGC ATGTTCATCG GCCAGTGGAC CATGGACAAG 
AAGGCCGGAT ACGGCGTCTT CGACGACATC ACCAGGGGAG AAAAGTACAT GGGCATGTGG 
CAAGATGACA TGTGTGAGGG CAACGGCGTG GTGGTCACTC AGTTCGGACT CTATTACGAA 
GGCGCCTTTA GCCTTAACAA AATGATGGGA ACTGGAATTT TGCTCTCTGA AGATGATACA 
GTCTACGAGG GAGAATTTTC AGATGACTGG ACCCTCAATG GAAAGGGGAC GCTGACCCTG 
CTGAATGGAG ATTACATCGA AGGCCATTTT AGCGGAGAAT GGGGGTCTGG GATCAAAATC 
ACCGGAACCT ACTTCAAACC CAGCCTGTAC GAGAGCGATA AGGAGCGGCC CAAGGCCGTG 
AAGCTCGGAA ACCTGGCCGT GGCGGCGGAC GAGAAGTGGA CGGCGGTGTT CGAGGAGTGC 
TGGCGACAGC TGGGCTGCGA GTCTCCCGGG CAGGGGGAGA GCTGGAAGGC CTGGGATAAC 
ATCGCCGTGG CCCTGACCGC CAACAGACGC CAGCACCGAG ACAGCCCCGA GATCCTAAGT 
CGTTCGCACA CTCAGACGCT GGAGAGCCTG GAATTCATCC CGCAGCACGG CGGGGACTTC 
ACGGTGGAGA AATACGAAGA CATCAAGAGG TATCTGATGA AGGCCTGCGA CACCCCTCTG 
CACCCTCTGG GGCAGCTGGT AGAGACCCTG GTGAGAGTGT ACAGGATGAC TTACGTGGGC 
GTGGGAGCCA ACCGACGGTT ACTACAGGAG GCCGTCAGAG AGATCCAGTC CTACCTGAAG 
CGCATCTTCC GGCTCGTCAG GTTCCTTTTT CCCGACCTTC CCGAAGAAGG CAGCACGATT 
CCACTCTCCT CCAGTGTCCC ACCGGAGAGG AAATCCTTCT GCACCGGAAA AGCCGATTCC 
AGATCTGAAT CTCCAGAGCC GGGCTACGTG GTGACGAGCT CCGGGCTGCT GCTTCCCGTG 
CTGCTGCCTC GGCTCTACCC GCCGCTGTTC ACGCTGTACG CCCTGGACAA CGAGCGGGAG 
GAAGACGCCT ACTGGGAGTG CGTCCTGCGC CTCAACAAGC AGCCCGACAC GGCGCTCCTC 
AGCTTCCTGG GAGTGCAGCG GAAGTTTTGG CCTGACAACG TGTCGCTCCT CGGAGACAGC 
AGAAAGGTGC TGCCGACCAC GAAGGAGGCC TGTTTCGCCT CCGCGGTGGA GTGTCTGCAG 
CAGATCAGCA CGACCTTCAC TCCGTCGGAC AAGTTGAAGG TGATCCAGCA GACCTTCGAG 
GAGATCTCCC AGAGCGTCCT GGCGTGGCTC CGAGAAGACT TCCTGTGGTC CATGGACGAC 
CTGTTTCCCG TGTTCCTCTA CGTGGTTCTC CGGGCCAGAA TCCGCAATTT GGGCTCGGAA 
GTCCACCTGA TCGAGGACCT GATGGACCCC TACCTGCAGC ACGGCGAGCA GGGCATCATG 
TTCACCACCC TGAAGGCCTG TTACTACCAG ATCCAGCGAG AGAAACTGAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016081940.1
CDS	1..3174
Translation	MPPFLILLYAHSSRRPKNGPQNRGPGPHPGCFPQASTVHGDDGDERLMLLGPPSLISRPLSGNLPEGDRARNDYSPAENLGNPSAIKPLHGSFITAAGRELGARQFAWKADAANNVQTRVVRRSLMATFSPECIARACVERTPISYDITIDRLYQALENIHSHGRFGEELESSHDDKIGQGRAVPLAADAANNVQTCVVRRSGNVLSRMDFLNKNQELLQELSETKDGDVQLLEMLNALFFLPIRRLHDYARALLKLATCFEVASPEYQTLHDSSSCYETLALHLGRKRKEAEATLSFWKTFPGKMTDSLRKPERRLICESSNRALALQHAGRFSVNWFILFNDALVHAQFSTHHIFPLSTLWVEPISEEGNSANGLKLTTPEEQFTLISSTPQEKTKWLRALSQAVDQALRGAADFPPYGSGAPRPDPPASRSASYTFYKASRLKEATYEGRWLSGKPHGRGVLKWTDGKTYSGMFRNGLEDGYGEYKVPNKALNKEDHYVGYWKEGKMCGQGVYSYATGEVYEGYFQENVRHGHGLLRSGKLTSTSPSMFIGQWTMDKKAGYGVFDDITRGEKYMGMWQDDMCEGNGVVVTQFGLYYEGAFSLNKMMGTGILLSEDDTVYEGEFSDDWTLNGKGTLTLLNGDYIEGHFSGEWGSGIKITGTYFKPSLYESDKERPKAVKLGNLAVAADEKWTAVFEECWRQLGCESPGQGESWKAWDNIAVALTANRRQHRDSPEILSRSHTQTLESLEFIPQHGGDFTVEKYEDIKRYLMKACDTPLHPLGQLVETLVRVYRMTYVGVGANRRLLQEAVREIQSYLKRIFRLVRFLFPDLPEEGSTIPLSSSVPPERKSFCTGKADSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFTLYALDNEREEDAYWECVLRLNKQPDTALLSFLGVQRKFWPDNVSLLGDSRKVLPTTKEACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLAWLREDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_016226454.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus alsin (LOC100088146), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016226454.1

ORF Insert Sequence:

ATGCCGCCGT TTTTAATTCT GCTCTACGCT CACTCTTCCA GGCGCCCCAA GAATGGCCCC 
CAGAACCGGG GACCAGGCCC TCATCCTGGG TGCTTCCCAC AGGCGTCTAC GGTCCACGGG 
GACGATGGAG ATGAAAGGTT GATGCTTTTG GGGCCGCCAT CTCTGATTTC ACGGCCCTTG 
AGTGGGAATC TCCCCGAAGG TGACAGAGCA CGGAATGACT ACTCCCCAGC TGAGAACCTG 
GGGAACCCAT CCGCTATTAA GCCTTTACAT GGTTCCTTCA TTACAGCAGC CGGGCGAGAG 
CTGGGAGCGC GGCAGTTTGC CTGGAAGGCC GATGCCGCGA ACAACGTCCA GACACGCGTG 
GTGCGCCGGA GTCTCATGGC AACGTTCTCT CCAGAATGTA TCGCAAGGGC ATGTGTTGAG 
AGAACGCCAA TTTCATATGA CATAACCATA GATCGGCTGT ATCAAGCGCT GGAAAATATT 
CACTCACATG GCCGGTTTGG CGAGGAGCTG GAATCCTCCC ATGACGATAA AATTGGTCAG 
GGCCGTGCAG TACCTTTAGC GGCCGATGCC GCGAACAACG TCCAGACATG CGTGGTGCGC 
CGGAGTGGCA ACGTTCTCTC CAGAATGGAC TTCTTAAATA AAAACCAGGA GCTGTTACAG 
GAGCTGTCGG AGACGAAAGA CGGGGACGTC CAGCTGTTGG AGATGCTGAA TGCCTTATTC 
TTCCTCCCAA TCCGGCGGCT TCATGATTAC GCCCGAGCTC TGCTAAAGCT GGCTACATGT 
TTTGAGGTGG CCTCTCCGGA GTACCAGACG CTGCACGATT CCAGTTCCTG CTACGAGACC 
CTCGCTCTCC ACCTTGGCAG GAAAAGGAAG GAAGCAGAAG CCACGCTGAG CTTCTGGAAG 
ACGTTCCCCG GAAAGATGAC GGACTCCTTG AGGAAGCCAG AGCGCAGGCT AATCTGCGAG 
AGCAGCAATC GAGCGCTGGC CCTGCAGCAT GCTGGGAGAT TCTCCGTGAA TTGGTTCATT 
CTCTTTAACG ATGCCTTGGT CCATGCCCAG TTCTCGACCC ATCACATCTT CCCCCTGTCC 
ACTCTGTGGG TCGAGCCTAT CTCAGAAGAA GGCAACAGCG CGAACGGCCT GAAGTTGACG 
ACGCCGGAGG AACAGTTCAC CCTCATTTCC TCGACCCCGC AGGAGAAGAC CAAGTGGCTG 
CGGGCCCTGA GCCAGGCGGT GGACCAGGCC CTGAGGGGCG CGGCCGACTT CCCCCCCTAC 
GGCAGCGGCG CCCCGCGGCC GGACCCCCCC GCCTCCCGCA GCGCCAGCTA CACCTTCTAC 
AAGGCCTCCC GCCTCAAGGA AGCCACCTAC GAGGGCCGCT GGCTCTCCGG GAAGCCCCAC 
GGCAGAGGGG TCTTGAAGTG GACCGACGGC AAGACGTACT CCGGTATGTT TCGGAATGGC 
CTGGAAGATG GATACGGGGA ATATAAAGTC CCAAACAAAG CACTTAACAA AGAAGACCAC 
TACGTCGGGT ACTGGAAAGA AGGAAAAATG TGCGGACAAG GAGTTTACAG CTACGCCACG 
GGCGAAGTGT ACGAAGGCTA CTTCCAAGAG AACGTCCGCC ACGGCCACGG GCTTCTCCGC 
AGCGGCAAGC TGACCTCCAC CTCCCCCAGC ATGTTCATCG GCCAGTGGAC CATGGACAAG 
AAGGCCGGAT ACGGCGTCTT CGACGACATC ACCAGGGGAG AAAAGTACAT GGGCATGTGG 
CAAGATGACA TGTGTGAGGG CAACGGCGTG GTGGTCACTC AGTTCGGACT CTATTACGAA 
GGCGCCTTTA GCCTTAACAA AATGATGGGA ACTGGAATTT TGCTCTCTGA AGATGATACA 
GTCTACGAGG GAGAATTTTC AGATGACTGG ACCCTCAATG GAAAGGGGAC GCTGACCCTG 
CTGAATGGAG ATTACATCGA AGGCCATTTT AGCGGAGAAT GGGGGTCTGG GATCAAAATC 
ACCGGAACCT ACTTCAAACC CAGCCTGTAC GAGAGCGATA AGGAGCGGCC CAAGGCCGTG 
AAGCTCGGAA ACCTGGCCGT GGCGGCGGAC GAGAAGTGGA CGGCGGTGTT CGAGGAGTGC 
TGGCGACAGC TGGGCTGCGA GTCTCCCGGG CAGGGGGAGA GCTGGAAGGC CTGGGATAAC 
ATCGCCGTGG CCCTGACCGC CAACAGACGC CAGCACCGAG ACAGCCCCGA GATCCTAAGT 
CGTTCGCACA CTCAGACGCT GGAGAGCCTG GAATTCATCC CGCAGCACGG CGGGGACTTC 
ACGGTGGAGA AATACGAAGA CATCAAGAGG TATCTGATGA AGGCCTGCGA CACCCCTCTG 
CACCCTCTGG GGCAGCTGGT AGAGACCCTG GTGAGAGTGT ACAGGATGAC TTACGTGGGC 
GTGGGAGCCA ACCGACGGTT ACTACAGGAG GCCGTCAGAG AGATCCAGTC CTACCTGAAG 
CGCATCTTCC GGCTCGTCAG GTTCCTTTTT CCCGACCTTC CCGAAGAAGG CAGCACGATT 
CCACTCTCCT CCAGTGTCCC ACCGGAGAGG AAATCCTTCT GCACCGGAAA AGCCGATTCC 
AGATCTGAAT CTCCAGAGCC GGGCTACGTG GTGACGAGCT CCGGGCTGCT GCTTCCCGTG 
CTGCTGCCTC GGCTCTACCC GCCGCTGTTC ACGCTGTACG CCCTGGACAA CGAGCGGGAG 
GAAGACGCCT ACTGGGAGTG CGTCCTGCGC CTCAACAAGC AGCCCGACAC GGCGCTCCTC 
AGCTTCCTGG GAGTGCAGCG GAAGTTTTGG CCTGACAACG TGTCGCTCCT CGGAGACAGC 
AGAAAGGTGC TGCCGACCAC GAAGGAGGCC TGTTTCGCCT CCGCGGTGGA GTGTCTGCAG 
CAGATCAGCA CGACCTTCAC TCCGTCGGAC AAGTTGAAGG TGATCCAGCA GACCTTCGAG 
GAGATCTCCC AGAGCGTCCT GGCGTGGCTC CGAGAAGACT TCCTGTGGTC CATGGACGAC 
CTGTTTCCCG TGTTCCTCTA CGTGGTTCTC CGGGCCAGAA TCCGCAATTT GGGCTCGGAA 
GTCCACCTGA TCGAGGACCT GATGGACCCC TACCTGCAGC ACGGCGAGCA GGGCATCATG 
TTCACCACCC TGAAGGCCTG TTACTACCAG ATCCAGCGAG AGAAACTGAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_029068795.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028924628.1 Ornithorhynchus anatinus alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOc131912
Clone ID Related Accession (Same CDS sequence)	XM_029068795.1 , XM_029068796.1 , XM_029068797.1
Accession Version	XM_029068795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556380800000
Organism	Ornithorhynchus anatinus(platypus)
Product	alsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCGC AGGAGAGCAG CTCAGCCGAG GCAGAAGGAG CCAGAGAGAG GGGCCTGGTC 
CGCGTTTGGT CGGCGAGGTC GTGCCGTGAG ACCCCAGAGA GGCTTCGGGG CTGGGGAGGG 
AAGACCGTCC TCCAAGCGGC CCTGGGCGTC AGTCACGGCG TCCTCCTGAC CGAAGGCGGC 
GAAGTGTACA GCTTCGGACA GCTGCCCTGG AAGCTCGAGG CGGAGGCGGA GGGACCGCGG 
CCGAGCGGCC CCGTCCTAGA ACATGCCCTG GTCGGGCAGC GCGTGGTGGC CGTGGCCACG 
GGCAGCTTCC ACAGCGGGGC CGTCACGGAG GCCGGCGCCG TGTACATGTG GGGGGAGAAC 
GGCGCGGGCC AGTGCGCCGT GGCCGAGCAG GCCCGCGTGC CCGAGCCCAG TCTCGTGGCC 
CTGGAGGACC CCGAGCCCGG CCCGCCGCTG CCCGTGCGCG TCCTGCAGCT GGCCTGCGGC 
GAGGAGCACA CCCTGGCCCT GTCCATCAGC CGGGAGATCT GGGCCTGGGG CACGGGCTGC 
CAGCTGGGCC TCCTGACGGC CTCCTTCCCG GTGACCAGGC CGCAGAAGGT GGAGCACCTG 
GCCGGGCGGG TGGTGCTGCA GGTGGCCTGC GGGCTCTCCC ACAGCCTGGC CCTGGTCCAG 
TGCCCGCCCG CCCTCGACCG GAAGCCGCTC CCCGAGAGAT GCCGCCACTG CAGCCAGCTC 
CTGATCACCA TGACGGACAA AGAAGACCAC GTCATCATAT CGGACAGTCA CTGCTGCCCT 
CTGGGAGTGG TCCTCCCCGA CGTCCAGGAG GACGGCGGCA CCGGGCCCAA GCCCCCGCTG 
GGGCCCCCGG ACGGGGGAGG CCCGGCCGAG GCCCCGGGCC AGGGCCGGGG AAGGGGCCCA 
GTGGGGGCCC ACGCGCCTGT GGCTCCCGAA GGGCCCGTCG GCCCGGCCCA GGGTGTGGGA 
GGGGAGGCCG AACCCTGCCC GCCGGAGGCG AGCGGGGCCC AGGGGCCGGA CGGGGCCGGG 
GGTGGGCCCA CAGACACCGA CGAGGGGCTG CGGAAGGCGT CGGATCCCTC AGGAAGAGAG 
GATGCCGGGA GAAGAGCCAA CGTGGAGCCG GCTCAGTTTC AACCTCTTAT GGAAGAAGCG 
AGCCCCTGCC TCCCCAGCCC CCCGACGCTG GGCTCGTCCG CCCTAAACAG CTTGGTGGTC 
TCCTGCGCCT CGGCCGTGGG GGTCCACATC GCCGCCACCT ACGAAGCCGG CGCGCTGTCG 
CTCAGGAAGG TGATGAACTT TTCCGGCGAG GCGGGCTCGC CGTCGGGCCC CGAGGGGCCG 
AGAGACTGCC GGGAGGAGCA GGTCAAACTG GAATCGCTGC AGGGCAAGAA GAGCTCCAGC 
CTCGTGGACA TCAGAGAGGA GGAAGCCGAG GGCAGCAGTC GGAGGCTTTC CCTGCCCGGG 
TTGCTCTCCC AAGTCTCCCC CAGGCTCTTG AGGAAGGCGG CGCGGGCCAA GCCGCGGACG 
GTGGTGCTGA CCCCGACGTA CAGCGGCGAA GCGGACGCGC TGCTCCCGTC GCTGAGGACG 
GAGGTGTGGA GCTGGGGGAA GGGGAAGGAA GGCCAGCTGG GGCACGGGGA TGTCCTGCCC 
AGGCTTCAGC CCCTGTGTGT AAAATGCCTC GATGGTAAAG AAGTCATCCA CCTGGCCGCC 
GGGGACCACC ATTCCCTGGC CCTCACAGCC AAATCCCAGG TCTTCTCCTG GGGAAGCAAT 
ACCTTTGGGC AGCTGGGACA CTCGGATTCT CCAACCACAG TTCCTCGTCT TGCAAAGGTG 
TGCGGTGAAA ACCGCATCTG GAGTTTCGCC GCCGGCCAGG ATTTCTCCCT CTTCCTAGTG 
GACACGGAAG ACTTCCAGCC TGGATTGTAT TACAGCGGCC GGCAGGACTC TCCAGAAGGT 
GACGGTGGCC CAGAGAACGC CGTCGCCACA AAGTCTCCCA GGCTGCTGAC GTCTTGTAGC 
AAGCTGGGCT ACATCAGCAG TGTGACAGCC GGCAGGGACA GCTGTCTCGC CCTGGTGGAC 
AAAAACATCA TGGGCTACAT CGCCAGCCTG CATGAGCTGG CTGCTACGGA AAGACGATTC 
TATTCCATGC TGAGCACCGT CAAATCCCAG GTTCTCCGAC CTCTTCTCAG TTTAGATAAC 
TTGGGCGCCC CAGCTTCGGT CCAGCTCCTT CAAGAGGTGG CCAGCAGGTT CAGCAAGCTG 
TGTTACCTGA TTGGGCAGCA CGCGGCCTCC TTGAGCATCT TCCTCCGCGG GATAAAAGAA 
GTGCGGAGCC TGACGGTCCT GAAGCATGCA AGTCTCTTCT TGGATAGTTA CACAGAGTAC 
TGCACGGCCC TGACCAATTT TATCGTCATG GGAGGATTCC AGCTCCTCGC CAAACCGGCC 
ATGGACTTCT TAAATAAAAA CCAGGAGCTG TTACAGGAGC TGTCGGAGAC GAAAGACGGG 
GACGTCCAGC TGTTGGAGAT GCTGAATGCC TTATTCTTCC TCCCAATCCG GCGGCTTCAT 
GATTACGCCC GAGCTCTGCT AAAGCTGGCT ACATGTTTTG AGGTGGCCTC TCCGGAGTAC 
CAGACGCTGC ACGATTCCAG TTCCTGCTAC GAGACCCTCG CTCTCCACCT TGGCAGGAAA 
AGGAAGGAAG CAGAAGCCAC GCTGAGCTTC TGGAAGACGT TCCCCGGAAA GATGACGGAC 
TCCTTGAGGA AGCCAGAGCG CAGGCTAATC TGCGAGAGCA GCAATCGAGC GCTGGCCCTG 
CAGCATGCTG GGAGATTCTC CGTGAATTGG TTCATTCTCT TTAACGATGC CTTGGTCCAT 
GCCCAGTTCT CGACCCATCA CATCTTCCCC CTGTCCACTC TGTGGGTCGA GCCTATCTCA 
GAAGAAGGCA ACAGCGCGAA CGGCCTGAAG TTGACGACGC CGGAGGAACA GTTCACCCTC 
ATTTCCTCGA CCCCGCAGGA GAAGACCAAG TGGCTGCGGG CCCTGAGCCA GGCGGTGGAC 
CAGGCCCTGA GGGGCGCGGC CGACTTCCCC CCCTACGGCA GCGGCGCCCC GCGGCCGGAC 
CCCCCCGCCT CCCGCAGCGC CAGCTACACC TTCTACAAGG CCTCCCGCCT CAAGGAAGCC 
ACCTACGAGG GCCGCTGGCT CTCCGGGAAG CCCCACGGCA GAGGGGTCTT GAAGTGGACC 
GACGGCAAGA CGTACTCCGG TATGTTTCGG AATGGCCTGG AAGATGGATA CGGGGAATAT 
AAAGTCCCAA ACAAAGCACT TAACAAAGAA GACCACTACG TCGGGTACTG GAAAGAAGGA 
AAAATGTGCG GACAAGGAGT TTACAGCTAC GCCACGGGCG AAGTGTACGA AGGCTACTTC 
CAAGAGAACG TCCGCCACGG CCACGGGCTT CTCCGCAGCG GCAAGCTGAC CTCCACCTCC 
CCCAGCATGT TCATCGGCCA GTGGACCATG GACAAGAAGG CCGGATACGG CGTCTTCGAC 
GACATCACCA GGGGAGAAAA GTACATGGGC ATGTGGCAAG ATGACATGTG TGAGGGCAAC 
GGCGTGGTGG TCACTCAGTT CGGACTCTAT TACGAAGGCG CCTTTAGCCT TAACAAAATG 
ATGGGAACTG GAATTTTGCT CTCTGAAGAT GATACAGTCT ACGAGGGAGA ATTTTCAGAT 
GACTGGACCC TCAATGGAAA GGGGACGCTG ACCCTGCTGA ATGGAGATTA CATCGAAGGC 
CATTTTAGCG GAGAATGGGG GTCTGGGATC AAAATCACCG GAACCTACTT CAAACCCAGC 
CTGTACGAGA GCGATAAGGA GCGGCCCAAG GCCGTGAAGC TCGGAAACCT GGCCGTGGCG 
GCGGACGAGA AGTGGACGGC GGTGTTCGAG GAGTGCTGGC GACAGCTGGG CTGCGAGTCT 
CCCGGGCAGG GGGAGAGCTG GAAGGCCTGG GATAACATCG CCGTGGCCCT GACCGCCAAC 
AGACGCCAGC ACCGAGACAG CCCCGAGATC CTAAGTCGTT CGCACACTCA GACGCTGGAG 
AGCCTGGAAT TCATCCCGCA GCACGGCGGG GACTTCACGG TGGAGAAATA CGAAGACATC 
AAGAGGTATC TGATGAAGGC CTGCGACACC CCTCTGCACC CTCTGGGGCA GCTGGTAGAG 
ACCCTGGTGG CAGTGTACAG GATGACTTAC GTGGGCGTGG GAGCCAACCG GCGGTTACTA 
CAGGAGGCCG TCAGAGAGAT CCAGTCCTAC CTGAAGCGCA TCTTCCAGCT TGTCAGGTTC 
CTTTTTCCCG ACCTTCCCGA AGAAGGCAGC ACGATTCCAC TCTCCTCCAG TGTCCCACCG 
GAGAGGAAAT CCTTCTGCAC CGGAAAAGCC GATTCCAGAT CTGAATCTCC AGAGCCGGGC 
TACGTGGTGA CGAGCTCCGG GCTGCTGCTT CCCGTGCTGC TGCCTCGGCT CTACCCGCCG 
CTGTTCACGC TGTACGCCCT GGACAACGAG CGGGAGGAAG ACGCCTACTG GGAGTGCGTC 
CTGCGCCTCA ACAAGCAGCC CGACACGGCG CTCCTCAGCT TCCTGGGAGT GCAGCGGAAG 
TTTTGGCCTG ACAACGTGTC GCTCCTCGGA GACAGCAGAA AGGTGCTGCC GACCACGAAG 
GAGGCCTGTT TCGCCTCCGC GGTGGAGTGT CTGCAGCAGA TCAGCACGAC CTTCACTCCG 
TCGGACAAGT TGAAGGTGAT CCAGCAGACC TTCGAGGAGA TCTCCCAGAG CGTCCTGGCG 
TGGCTCCGAG AAGACTTCCT GTGGTCCATG GACGACCTGT TTCCCGTGTT CCTCTACGTG 
GTTCTCCGGG CCAGAATCCG CAATTTGGGC TCGGAAGTCC ACCTGATCGA GGACCTGATG 
GACCCCTACC TGCAGCACGG CGAGCAGGGC ATCATGTTCA CCACCCTGAA GGCCTGTTAC 
TACCAGATCC AGCGAGAGAA ACTGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028924628.1
CDS	324..5273
Translation	MDPQESSSAEAEGARERGLVRVWSARSCRETPERLRGWGGKTVLQAALGVSHGVLLTEGGEVYSFGQLPWKLEAEAEGPRPSGPVLEHALVGQRVVAVATGSFHSGAVTEAGAVYMWGENGAGQCAVAEQARVPEPSLVALEDPEPGPPLPVRVLQLACGEEHTLALSISREIWAWGTGCQLGLLTASFPVTRPQKVEHLAGRVVLQVACGLSHSLALVQCPPALDRKPLPERCRHCSQLLITMTDKEDHVIISDSHCCPLGVVLPDVQEDGGTGPKPPLGPPDGGGPAEAPGQGRGRGPVGAHAPVAPEGPVGPAQGVGGEAEPCPPEASGAQGPDGAGGGPTDTDEGLRKASDPSGREDAGRRANVEPAQFQPLMEEASPCLPSPPTLGSSALNSLVVSCASAVGVHIAATYEAGALSLRKVMNFSGEAGSPSGPEGPRDCREEQVKLESLQGKKSSSLVDIREEEAEGSSRRLSLPGLLSQVSPRLLRKAARAKPRTVVLTPTYSGEADALLPSLRTEVWSWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLAAGDHHSLALTAKSQVFSWGSNTFGQLGHSDSPTTVPRLAKVCGENRIWSFAAGQDFSLFLVDTEDFQPGLYYSGRQDSPEGDGGPENAVATKSPRLLTSCSKLGYISSVTAGRDSCLALVDKNIMGYIASLHELAATERRFYSMLSTVKSQVLRPLLSLDNLGAPASVQLLQEVASRFSKLCYLIGQHAASLSIFLRGIKEVRSLTVLKHASLFLDSYTEYCTALTNFIVMGGFQLLAKPAMDFLNKNQELLQELSETKDGDVQLLEMLNALFFLPIRRLHDYARALLKLATCFEVASPEYQTLHDSSSCYETLALHLGRKRKEAEATLSFWKTFPGKMTDSLRKPERRLICESSNRALALQHAGRFSVNWFILFNDALVHAQFSTHHIFPLSTLWVEPISEEGNSANGLKLTTPEEQFTLISSTPQEKTKWLRALSQAVDQALRGAADFPPYGSGAPRPDPPASRSASYTFYKASRLKEATYEGRWLSGKPHGRGVLKWTDGKTYSGMFRNGLEDGYGEYKVPNKALNKEDHYVGYWKEGKMCGQGVYSYATGEVYEGYFQENVRHGHGLLRSGKLTSTSPSMFIGQWTMDKKAGYGVFDDITRGEKYMGMWQDDMCEGNGVVVTQFGLYYEGAFSLNKMMGTGILLSEDDTVYEGEFSDDWTLNGKGTLTLLNGDYIEGHFSGEWGSGIKITGTYFKPSLYESDKERPKAVKLGNLAVAADEKWTAVFEECWRQLGCESPGQGESWKAWDNIAVALTANRRQHRDSPEILSRSHTQTLESLEFIPQHGGDFTVEKYEDIKRYLMKACDTPLHPLGQLVETLVAVYRMTYVGVGANRRLLQEAVREIQSYLKRIFQLVRFLFPDLPEEGSTIPLSSSVPPERKSFCTGKADSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFTLYALDNEREEDAYWECVLRLNKQPDTALLSFLGVQRKFWPDNVSLLGDSRKVLPTTKEACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLAWLREDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_029068795.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029068795.1

ORF Insert Sequence:

ATGGACCCGC AGGAGAGCAG CTCAGCCGAG GCAGAAGGAG CCAGAGAGAG GGGCCTGGTC 
CGCGTTTGGT CGGCGAGGTC GTGCCGTGAG ACCCCAGAGA GGCTTCGGGG CTGGGGAGGG 
AAGACCGTCC TCCAAGCGGC CCTGGGCGTC AGTCACGGCG TCCTCCTGAC CGAAGGCGGC 
GAAGTGTACA GCTTCGGACA GCTGCCCTGG AAGCTCGAGG CGGAGGCGGA GGGACCGCGG 
CCGAGCGGCC CCGTCCTAGA ACATGCCCTG GTCGGGCAGC GCGTGGTGGC CGTGGCCACG 
GGCAGCTTCC ACAGCGGGGC CGTCACGGAG GCCGGCGCCG TGTACATGTG GGGGGAGAAC 
GGCGCGGGCC AGTGCGCCGT GGCCGAGCAG GCCCGCGTGC CCGAGCCCAG TCTCGTGGCC 
CTGGAGGACC CCGAGCCCGG CCCGCCGCTG CCCGTGCGCG TCCTGCAGCT GGCCTGCGGC 
GAGGAGCACA CCCTGGCCCT GTCCATCAGC CGGGAGATCT GGGCCTGGGG CACGGGCTGC 
CAGCTGGGCC TCCTGACGGC CTCCTTCCCG GTGACCAGGC CGCAGAAGGT GGAGCACCTG 
GCCGGGCGGG TGGTGCTGCA GGTGGCCTGC GGGCTCTCCC ACAGCCTGGC CCTGGTCCAG 
TGCCCGCCCG CCCTCGACCG GAAGCCGCTC CCCGAGAGAT GCCGCCACTG CAGCCAGCTC 
CTGATCACCA TGACGGACAA AGAAGACCAC GTCATCATAT CGGACAGTCA CTGCTGCCCT 
CTGGGAGTGG TCCTCCCCGA CGTCCAGGAG GACGGCGGCA CCGGGCCCAA GCCCCCGCTG 
GGGCCCCCGG ACGGGGGAGG CCCGGCCGAG GCCCCGGGCC AGGGCCGGGG AAGGGGCCCA 
GTGGGGGCCC ACGCGCCTGT GGCTCCCGAA GGGCCCGTCG GCCCGGCCCA GGGTGTGGGA 
GGGGAGGCCG AACCCTGCCC GCCGGAGGCG AGCGGGGCCC AGGGGCCGGA CGGGGCCGGG 
GGTGGGCCCA CAGACACCGA CGAGGGGCTG CGGAAGGCGT CGGATCCCTC AGGAAGAGAG 
GATGCCGGGA GAAGAGCCAA CGTGGAGCCG GCTCAGTTTC AACCTCTTAT GGAAGAAGCG 
AGCCCCTGCC TCCCCAGCCC CCCGACGCTG GGCTCGTCCG CCCTAAACAG CTTGGTGGTC 
TCCTGCGCCT CGGCCGTGGG GGTCCACATC GCCGCCACCT ACGAAGCCGG CGCGCTGTCG 
CTCAGGAAGG TGATGAACTT TTCCGGCGAG GCGGGCTCGC CGTCGGGCCC CGAGGGGCCG 
AGAGACTGCC GGGAGGAGCA GGTCAAACTG GAATCGCTGC AGGGCAAGAA GAGCTCCAGC 
CTCGTGGACA TCAGAGAGGA GGAAGCCGAG GGCAGCAGTC GGAGGCTTTC CCTGCCCGGG 
TTGCTCTCCC AAGTCTCCCC CAGGCTCTTG AGGAAGGCGG CGCGGGCCAA GCCGCGGACG 
GTGGTGCTGA CCCCGACGTA CAGCGGCGAA GCGGACGCGC TGCTCCCGTC GCTGAGGACG 
GAGGTGTGGA GCTGGGGGAA GGGGAAGGAA GGCCAGCTGG GGCACGGGGA TGTCCTGCCC 
AGGCTTCAGC CCCTGTGTGT AAAATGCCTC GATGGTAAAG AAGTCATCCA CCTGGCCGCC 
GGGGACCACC ATTCCCTGGC CCTCACAGCC AAATCCCAGG TCTTCTCCTG GGGAAGCAAT 
ACCTTTGGGC AGCTGGGACA CTCGGATTCT CCAACCACAG TTCCTCGTCT TGCAAAGGTG 
TGCGGTGAAA ACCGCATCTG GAGTTTCGCC GCCGGCCAGG ATTTCTCCCT CTTCCTAGTG 
GACACGGAAG ACTTCCAGCC TGGATTGTAT TACAGCGGCC GGCAGGACTC TCCAGAAGGT 
GACGGTGGCC CAGAGAACGC CGTCGCCACA AAGTCTCCCA GGCTGCTGAC GTCTTGTAGC 
AAGCTGGGCT ACATCAGCAG TGTGACAGCC GGCAGGGACA GCTGTCTCGC CCTGGTGGAC 
AAAAACATCA TGGGCTACAT CGCCAGCCTG CATGAGCTGG CTGCTACGGA AAGACGATTC 
TATTCCATGC TGAGCACCGT CAAATCCCAG GTTCTCCGAC CTCTTCTCAG TTTAGATAAC 
TTGGGCGCCC CAGCTTCGGT CCAGCTCCTT CAAGAGGTGG CCAGCAGGTT CAGCAAGCTG 
TGTTACCTGA TTGGGCAGCA CGCGGCCTCC TTGAGCATCT TCCTCCGCGG GATAAAAGAA 
GTGCGGAGCC TGACGGTCCT GAAGCATGCA AGTCTCTTCT TGGATAGTTA CACAGAGTAC 
TGCACGGCCC TGACCAATTT TATCGTCATG GGAGGATTCC AGCTCCTCGC CAAACCGGCC 
ATGGACTTCT TAAATAAAAA CCAGGAGCTG TTACAGGAGC TGTCGGAGAC GAAAGACGGG 
GACGTCCAGC TGTTGGAGAT GCTGAATGCC TTATTCTTCC TCCCAATCCG GCGGCTTCAT 
GATTACGCCC GAGCTCTGCT AAAGCTGGCT ACATGTTTTG AGGTGGCCTC TCCGGAGTAC 
CAGACGCTGC ACGATTCCAG TTCCTGCTAC GAGACCCTCG CTCTCCACCT TGGCAGGAAA 
AGGAAGGAAG CAGAAGCCAC GCTGAGCTTC TGGAAGACGT TCCCCGGAAA GATGACGGAC 
TCCTTGAGGA AGCCAGAGCG CAGGCTAATC TGCGAGAGCA GCAATCGAGC GCTGGCCCTG 
CAGCATGCTG GGAGATTCTC CGTGAATTGG TTCATTCTCT TTAACGATGC CTTGGTCCAT 
GCCCAGTTCT CGACCCATCA CATCTTCCCC CTGTCCACTC TGTGGGTCGA GCCTATCTCA 
GAAGAAGGCA ACAGCGCGAA CGGCCTGAAG TTGACGACGC CGGAGGAACA GTTCACCCTC 
ATTTCCTCGA CCCCGCAGGA GAAGACCAAG TGGCTGCGGG CCCTGAGCCA GGCGGTGGAC 
CAGGCCCTGA GGGGCGCGGC CGACTTCCCC CCCTACGGCA GCGGCGCCCC GCGGCCGGAC 
CCCCCCGCCT CCCGCAGCGC CAGCTACACC TTCTACAAGG CCTCCCGCCT CAAGGAAGCC 
ACCTACGAGG GCCGCTGGCT CTCCGGGAAG CCCCACGGCA GAGGGGTCTT GAAGTGGACC 
GACGGCAAGA CGTACTCCGG TATGTTTCGG AATGGCCTGG AAGATGGATA CGGGGAATAT 
AAAGTCCCAA ACAAAGCACT TAACAAAGAA GACCACTACG TCGGGTACTG GAAAGAAGGA 
AAAATGTGCG GACAAGGAGT TTACAGCTAC GCCACGGGCG AAGTGTACGA AGGCTACTTC 
CAAGAGAACG TCCGCCACGG CCACGGGCTT CTCCGCAGCG GCAAGCTGAC CTCCACCTCC 
CCCAGCATGT TCATCGGCCA GTGGACCATG GACAAGAAGG CCGGATACGG CGTCTTCGAC 
GACATCACCA GGGGAGAAAA GTACATGGGC ATGTGGCAAG ATGACATGTG TGAGGGCAAC 
GGCGTGGTGG TCACTCAGTT CGGACTCTAT TACGAAGGCG CCTTTAGCCT TAACAAAATG 
ATGGGAACTG GAATTTTGCT CTCTGAAGAT GATACAGTCT ACGAGGGAGA ATTTTCAGAT 
GACTGGACCC TCAATGGAAA GGGGACGCTG ACCCTGCTGA ATGGAGATTA CATCGAAGGC 
CATTTTAGCG GAGAATGGGG GTCTGGGATC AAAATCACCG GAACCTACTT CAAACCCAGC 
CTGTACGAGA GCGATAAGGA GCGGCCCAAG GCCGTGAAGC TCGGAAACCT GGCCGTGGCG 
GCGGACGAGA AGTGGACGGC GGTGTTCGAG GAGTGCTGGC GACAGCTGGG CTGCGAGTCT 
CCCGGGCAGG GGGAGAGCTG GAAGGCCTGG GATAACATCG CCGTGGCCCT GACCGCCAAC 
AGACGCCAGC ACCGAGACAG CCCCGAGATC CTAAGTCGTT CGCACACTCA GACGCTGGAG 
AGCCTGGAAT TCATCCCGCA GCACGGCGGG GACTTCACGG TGGAGAAATA CGAAGACATC 
AAGAGGTATC TGATGAAGGC CTGCGACACC CCTCTGCACC CTCTGGGGCA GCTGGTAGAG 
ACCCTGGTGG CAGTGTACAG GATGACTTAC GTGGGCGTGG GAGCCAACCG GCGGTTACTA 
CAGGAGGCCG TCAGAGAGAT CCAGTCCTAC CTGAAGCGCA TCTTCCAGCT TGTCAGGTTC 
CTTTTTCCCG ACCTTCCCGA AGAAGGCAGC ACGATTCCAC TCTCCTCCAG TGTCCCACCG 
GAGAGGAAAT CCTTCTGCAC CGGAAAAGCC GATTCCAGAT CTGAATCTCC AGAGCCGGGC 
TACGTGGTGA CGAGCTCCGG GCTGCTGCTT CCCGTGCTGC TGCCTCGGCT CTACCCGCCG 
CTGTTCACGC TGTACGCCCT GGACAACGAG CGGGAGGAAG ACGCCTACTG GGAGTGCGTC 
CTGCGCCTCA ACAAGCAGCC CGACACGGCG CTCCTCAGCT TCCTGGGAGT GCAGCGGAAG 
TTTTGGCCTG ACAACGTGTC GCTCCTCGGA GACAGCAGAA AGGTGCTGCC GACCACGAAG 
GAGGCCTGTT TCGCCTCCGC GGTGGAGTGT CTGCAGCAGA TCAGCACGAC CTTCACTCCG 
TCGGACAAGT TGAAGGTGAT CCAGCAGACC TTCGAGGAGA TCTCCCAGAG CGTCCTGGCG 
TGGCTCCGAG AAGACTTCCT GTGGTCCATG GACGACCTGT TTCCCGTGTT CCTCTACGTG 
GTTCTCCGGG CCAGAATCCG CAATTTGGGC TCGGAAGTCC ACCTGATCGA GGACCTGATG 
GACCCCTACC TGCAGCACGG CGAGCAGGGC ATCATGTTCA CCACCCTGAA GGCCTGTTAC 
TACCAGATCC AGCGAGAGAA ACTGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_029068796.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028924629.1 Ornithorhynchus anatinus alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X2, mRNA.

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CloneID	OOc131912
Clone ID Related Accession (Same CDS sequence)	XM_029068795.1 , XM_029068796.1 , XM_029068797.1
Accession Version	XM_029068796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556380800000
Organism	Ornithorhynchus anatinus(platypus)
Product	alsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCGC AGGAGAGCAG CTCAGCCGAG GCAGAAGGAG CCAGAGAGAG GGGCCTGGTC 
CGCGTTTGGT CGGCGAGGTC GTGCCGTGAG ACCCCAGAGA GGCTTCGGGG CTGGGGAGGG 
AAGACCGTCC TCCAAGCGGC CCTGGGCGTC AGTCACGGCG TCCTCCTGAC CGAAGGCGGC 
GAAGTGTACA GCTTCGGACA GCTGCCCTGG AAGCTCGAGG CGGAGGCGGA GGGACCGCGG 
CCGAGCGGCC CCGTCCTAGA ACATGCCCTG GTCGGGCAGC GCGTGGTGGC CGTGGCCACG 
GGCAGCTTCC ACAGCGGGGC CGTCACGGAG GCCGGCGCCG TGTACATGTG GGGGGAGAAC 
GGCGCGGGCC AGTGCGCCGT GGCCGAGCAG GCCCGCGTGC CCGAGCCCAG TCTCGTGGCC 
CTGGAGGACC CCGAGCCCGG CCCGCCGCTG CCCGTGCGCG TCCTGCAGCT GGCCTGCGGC 
GAGGAGCACA CCCTGGCCCT GTCCATCAGC CGGGAGATCT GGGCCTGGGG CACGGGCTGC 
CAGCTGGGCC TCCTGACGGC CTCCTTCCCG GTGACCAGGC CGCAGAAGGT GGAGCACCTG 
GCCGGGCGGG TGGTGCTGCA GGTGGCCTGC GGGCTCTCCC ACAGCCTGGC CCTGGTCCAG 
TGCCCGCCCG CCCTCGACCG GAAGCCGCTC CCCGAGAGAT GCCGCCACTG CAGCCAGCTC 
CTGATCACCA TGACGGACAA AGAAGACCAC GTCATCATAT CGGACAGTCA CTGCTGCCCT 
CTGGGAGTGG TCCTCCCCGA CGTCCAGGAG GACGGCGGCA CCGGGCCCAA GCCCCCGCTG 
GGGCCCCCGG ACGGGGGAGG CCCGGCCGAG GCCCCGGGCC AGGGCCGGGG AAGGGGCCCA 
GTGGGGGCCC ACGCGCCTGT GGCTCCCGAA GGGCCCGTCG GCCCGGCCCA GGGTGTGGGA 
GGGGAGGCCG AACCCTGCCC GCCGGAGGCG AGCGGGGCCC AGGGGCCGGA CGGGGCCGGG 
GGTGGGCCCA CAGACACCGA CGAGGGGCTG CGGAAGGCGT CGGATCCCTC AGGAAGAGAG 
GATGCCGGGA GAAGAGCCAA CGTGGAGCCG GCTCAGTTTC AACCTCTTAT GGAAGAAGCG 
AGCCCCTGCC TCCCCAGCCC CCCGACGCTG GGCTCGTCCG CCCTAAACAG CTTGGTGGTC 
TCCTGCGCCT CGGCCGTGGG GGTCCACATC GCCGCCACCT ACGAAGCCGG CGCGCTGTCG 
CTCAGGAAGG TGATGAACTT TTCCGGCGAG GCGGGCTCGC CGTCGGGCCC CGAGGGGCCG 
AGAGACTGCC GGGAGGAGCA GGTCAAACTG GAATCGCTGC AGGGCAAGAA GAGCTCCAGC 
CTCGTGGACA TCAGAGAGGA GGAAGCCGAG GGCAGCAGTC GGAGGCTTTC CCTGCCCGGG 
TTGCTCTCCC AAGTCTCCCC CAGGCTCTTG AGGAAGGCGG CGCGGGCCAA GCCGCGGACG 
GTGGTGCTGA CCCCGACGTA CAGCGGCGAA GCGGACGCGC TGCTCCCGTC GCTGAGGACG 
GAGGTGTGGA GCTGGGGGAA GGGGAAGGAA GGCCAGCTGG GGCACGGGGA TGTCCTGCCC 
AGGCTTCAGC CCCTGTGTGT AAAATGCCTC GATGGTAAAG AAGTCATCCA CCTGGCCGCC 
GGGGACCACC ATTCCCTGGC CCTCACAGCC AAATCCCAGG TCTTCTCCTG GGGAAGCAAT 
ACCTTTGGGC AGCTGGGACA CTCGGATTCT CCAACCACAG TTCCTCGTCT TGCAAAGGTG 
TGCGGTGAAA ACCGCATCTG GAGTTTCGCC GCCGGCCAGG ATTTCTCCCT CTTCCTAGTG 
GACACGGAAG ACTTCCAGCC TGGATTGTAT TACAGCGGCC GGCAGGACTC TCCAGAAGGT 
GACGGTGGCC CAGAGAACGC CGTCGCCACA AAGTCTCCCA GGCTGCTGAC GTCTTGTAGC 
AAGCTGGGCT ACATCAGCAG TGTGACAGCC GGCAGGGACA GCTGTCTCGC CCTGGTGGAC 
AAAAACATCA TGGGCTACAT CGCCAGCCTG CATGAGCTGG CTGCTACGGA AAGACGATTC 
TATTCCATGC TGAGCACCGT CAAATCCCAG GTTCTCCGAC CTCTTCTCAG TTTAGATAAC 
TTGGGCGCCC CAGCTTCGGT CCAGCTCCTT CAAGAGGTGG CCAGCAGGTT CAGCAAGCTG 
TGTTACCTGA TTGGGCAGCA CGCGGCCTCC TTGAGCATCT TCCTCCGCGG GATAAAAGAA 
GTGCGGAGCC TGACGGTCCT GAAGCATGCA AGTCTCTTCT TGGATAGTTA CACAGAGTAC 
TGCACGGCCC TGACCAATTT TATCGTCATG GGAGGATTCC AGCTCCTCGC CAAACCGGCC 
ATGGACTTCT TAAATAAAAA CCAGGAGCTG TTACAGGAGC TGTCGGAGAC GAAAGACGGG 
GACGTCCAGC TGTTGGAGAT GCTGAATGCC TTATTCTTCC TCCCAATCCG GCGGCTTCAT 
GATTACGCCC GAGCTCTGCT AAAGCTGGCT ACATGTTTTG AGGTGGCCTC TCCGGAGTAC 
CAGACGCTGC ACGATTCCAG TTCCTGCTAC GAGACCCTCG CTCTCCACCT TGGCAGGAAA 
AGGAAGGAAG CAGAAGCCAC GCTGAGCTTC TGGAAGACGT TCCCCGGAAA GATGACGGAC 
TCCTTGAGGA AGCCAGAGCG CAGGCTAATC TGCGAGAGCA GCAATCGAGC GCTGGCCCTG 
CAGCATGCTG GGAGATTCTC CGTGAATTGG TTCATTCTCT TTAACGATGC CTTGGTCCAT 
GCCCAGTTCT CGACCCATCA CATCTTCCCC CTGTCCACTC TGTGGGTCGA GCCTATCTCA 
GAAGAAGGCA ACAGCGCGAA CGGCCTGAAG TTGACGACGC CGGAGGAACA GTTCACCCTC 
ATTTCCTCGA CCCCGCAGGA GAAGACCAAG TGGCTGCGGG CCCTGAGCCA GGCGGTGGAC 
CAGGCCCTGA GGGGCGCGGC CGACTTCCCC CCCTACGGCA GCGGCGCCCC GCGGCCGGAC 
CCCCCCGCCT CCCGCAGCGC CAGCTACACC TTCTACAAGG CCTCCCGCCT CAAGGAAGCC 
ACCTACGAGG GCCGCTGGCT CTCCGGGAAG CCCCACGGCA GAGGGGTCTT GAAGTGGACC 
GACGGCAAGA CGTACTCCGG TATGTTTCGG AATGGCCTGG AAGATGGATA CGGGGAATAT 
AAAGTCCCAA ACAAAGCACT TAACAAAGAA GACCACTACG TCGGGTACTG GAAAGAAGGA 
AAAATGTGCG GACAAGGAGT TTACAGCTAC GCCACGGGCG AAGTGTACGA AGGCTACTTC 
CAAGAGAACG TCCGCCACGG CCACGGGCTT CTCCGCAGCG GCAAGCTGAC CTCCACCTCC 
CCCAGCATGT TCATCGGCCA GTGGACCATG GACAAGAAGG CCGGATACGG CGTCTTCGAC 
GACATCACCA GGGGAGAAAA GTACATGGGC ATGTGGCAAG ATGACATGTG TGAGGGCAAC 
GGCGTGGTGG TCACTCAGTT CGGACTCTAT TACGAAGGCG CCTTTAGCCT TAACAAAATG 
ATGGGAACTG GAATTTTGCT CTCTGAAGAT GATACAGTCT ACGAGGGAGA ATTTTCAGAT 
GACTGGACCC TCAATGGAAA GGGGACGCTG ACCCTGCTGA ATGGAGATTA CATCGAAGGC 
CATTTTAGCG GAGAATGGGG GTCTGGGATC AAAATCACCG GAACCTACTT CAAACCCAGC 
CTGTACGAGA GCGATAAGGA GCGGCCCAAG GCCGTGAAGC TCGGAAACCT GGCCGTGGCG 
GCGGACGAGA AGTGGACGGC GGTGTTCGAG GAGTGCTGGC GACAGCTGGG CTGCGAGTCT 
CCCGGGCAGG GGGAGAGCTG GAAGGCCTGG GATAACATCG CCGTGGCCCT GACCGCCAAC 
AGACGCCAGC ACCGAGACAG CCCCGAGATC CTAAGTCGTT CGCACACTCA GACGCTGGAG 
AGCCTGGAAT TCATCCCGCA GCACGGCGGG GACTTCACGG TGGAGAAATA CGAAGACATC 
AAGAGGTATC TGATGAAGGC CTGCGACACC CCTCTGCACC CTCTGGGGCA GCTGGTAGAG 
ACCCTGGTGG CAGTGTACAG GATGACTTAC GTGGGCGTGG GAGCCAACCG GCGGTTACTA 
CAGGAGGCCG TCAGAGAGAT CCAGTCCTAC CTGAAGCGCA TCTTCCAGCT TGTCAGGTTC 
CTTTTTCCCG ACCTTCCCGA AGAAGGCAGC ACGATTCCAC TCTCCTCCAG TGTCCCACCG 
GAGAGGAAAT CCTTCTGCAC CGGAAAAGCC GATTCCAGAT CTGAATCTCC AGAGCCGGGC 
TACGTGGTGA CGAGCTCCGG GCTGCTGCTT CCCGTGCTGC TGCCTCGGCT CTACCCGCCG 
CTGTTCACGC TGTACGCCCT GGACAACGAG CGGGAGGAAG ACGCCTACTG GGAGTGCGTC 
CTGCGCCTCA ACAAGCAGCC CGACACGGCG CTCCTCAGCT TCCTGGGAGT GCAGCGGAAG 
TTTTGGCCTG ACAACGTGTC GCTCCTCGGA GACAGCAGAA AGGTGCTGCC GACCACGAAG 
GAGGCCTGTT TCGCCTCCGC GGTGGAGTGT CTGCAGCAGA TCAGCACGAC CTTCACTCCG 
TCGGACAAGT TGAAGGTGAT CCAGCAGACC TTCGAGGAGA TCTCCCAGAG CGTCCTGGCG 
TGGCTCCGAG AAGACTTCCT GTGGTCCATG GACGACCTGT TTCCCGTGTT CCTCTACGTG 
GTTCTCCGGG CCAGAATCCG CAATTTGGGC TCGGAAGTCC ACCTGATCGA GGACCTGATG 
GACCCCTACC TGCAGCACGG CGAGCAGGGC ATCATGTTCA CCACCCTGAA GGCCTGTTAC 
TACCAGATCC AGCGAGAGAA ACTGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028924629.1
CDS	123..5072
Translation	MDPQESSSAEAEGARERGLVRVWSARSCRETPERLRGWGGKTVLQAALGVSHGVLLTEGGEVYSFGQLPWKLEAEAEGPRPSGPVLEHALVGQRVVAVATGSFHSGAVTEAGAVYMWGENGAGQCAVAEQARVPEPSLVALEDPEPGPPLPVRVLQLACGEEHTLALSISREIWAWGTGCQLGLLTASFPVTRPQKVEHLAGRVVLQVACGLSHSLALVQCPPALDRKPLPERCRHCSQLLITMTDKEDHVIISDSHCCPLGVVLPDVQEDGGTGPKPPLGPPDGGGPAEAPGQGRGRGPVGAHAPVAPEGPVGPAQGVGGEAEPCPPEASGAQGPDGAGGGPTDTDEGLRKASDPSGREDAGRRANVEPAQFQPLMEEASPCLPSPPTLGSSALNSLVVSCASAVGVHIAATYEAGALSLRKVMNFSGEAGSPSGPEGPRDCREEQVKLESLQGKKSSSLVDIREEEAEGSSRRLSLPGLLSQVSPRLLRKAARAKPRTVVLTPTYSGEADALLPSLRTEVWSWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLAAGDHHSLALTAKSQVFSWGSNTFGQLGHSDSPTTVPRLAKVCGENRIWSFAAGQDFSLFLVDTEDFQPGLYYSGRQDSPEGDGGPENAVATKSPRLLTSCSKLGYISSVTAGRDSCLALVDKNIMGYIASLHELAATERRFYSMLSTVKSQVLRPLLSLDNLGAPASVQLLQEVASRFSKLCYLIGQHAASLSIFLRGIKEVRSLTVLKHASLFLDSYTEYCTALTNFIVMGGFQLLAKPAMDFLNKNQELLQELSETKDGDVQLLEMLNALFFLPIRRLHDYARALLKLATCFEVASPEYQTLHDSSSCYETLALHLGRKRKEAEATLSFWKTFPGKMTDSLRKPERRLICESSNRALALQHAGRFSVNWFILFNDALVHAQFSTHHIFPLSTLWVEPISEEGNSANGLKLTTPEEQFTLISSTPQEKTKWLRALSQAVDQALRGAADFPPYGSGAPRPDPPASRSASYTFYKASRLKEATYEGRWLSGKPHGRGVLKWTDGKTYSGMFRNGLEDGYGEYKVPNKALNKEDHYVGYWKEGKMCGQGVYSYATGEVYEGYFQENVRHGHGLLRSGKLTSTSPSMFIGQWTMDKKAGYGVFDDITRGEKYMGMWQDDMCEGNGVVVTQFGLYYEGAFSLNKMMGTGILLSEDDTVYEGEFSDDWTLNGKGTLTLLNGDYIEGHFSGEWGSGIKITGTYFKPSLYESDKERPKAVKLGNLAVAADEKWTAVFEECWRQLGCESPGQGESWKAWDNIAVALTANRRQHRDSPEILSRSHTQTLESLEFIPQHGGDFTVEKYEDIKRYLMKACDTPLHPLGQLVETLVAVYRMTYVGVGANRRLLQEAVREIQSYLKRIFQLVRFLFPDLPEEGSTIPLSSSVPPERKSFCTGKADSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFTLYALDNEREEDAYWECVLRLNKQPDTALLSFLGVQRKFWPDNVSLLGDSRKVLPTTKEACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLAWLREDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_029068796.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029068796.1

ORF Insert Sequence:

ATGGACCCGC AGGAGAGCAG CTCAGCCGAG GCAGAAGGAG CCAGAGAGAG GGGCCTGGTC 
CGCGTTTGGT CGGCGAGGTC GTGCCGTGAG ACCCCAGAGA GGCTTCGGGG CTGGGGAGGG 
AAGACCGTCC TCCAAGCGGC CCTGGGCGTC AGTCACGGCG TCCTCCTGAC CGAAGGCGGC 
GAAGTGTACA GCTTCGGACA GCTGCCCTGG AAGCTCGAGG CGGAGGCGGA GGGACCGCGG 
CCGAGCGGCC CCGTCCTAGA ACATGCCCTG GTCGGGCAGC GCGTGGTGGC CGTGGCCACG 
GGCAGCTTCC ACAGCGGGGC CGTCACGGAG GCCGGCGCCG TGTACATGTG GGGGGAGAAC 
GGCGCGGGCC AGTGCGCCGT GGCCGAGCAG GCCCGCGTGC CCGAGCCCAG TCTCGTGGCC 
CTGGAGGACC CCGAGCCCGG CCCGCCGCTG CCCGTGCGCG TCCTGCAGCT GGCCTGCGGC 
GAGGAGCACA CCCTGGCCCT GTCCATCAGC CGGGAGATCT GGGCCTGGGG CACGGGCTGC 
CAGCTGGGCC TCCTGACGGC CTCCTTCCCG GTGACCAGGC CGCAGAAGGT GGAGCACCTG 
GCCGGGCGGG TGGTGCTGCA GGTGGCCTGC GGGCTCTCCC ACAGCCTGGC CCTGGTCCAG 
TGCCCGCCCG CCCTCGACCG GAAGCCGCTC CCCGAGAGAT GCCGCCACTG CAGCCAGCTC 
CTGATCACCA TGACGGACAA AGAAGACCAC GTCATCATAT CGGACAGTCA CTGCTGCCCT 
CTGGGAGTGG TCCTCCCCGA CGTCCAGGAG GACGGCGGCA CCGGGCCCAA GCCCCCGCTG 
GGGCCCCCGG ACGGGGGAGG CCCGGCCGAG GCCCCGGGCC AGGGCCGGGG AAGGGGCCCA 
GTGGGGGCCC ACGCGCCTGT GGCTCCCGAA GGGCCCGTCG GCCCGGCCCA GGGTGTGGGA 
GGGGAGGCCG AACCCTGCCC GCCGGAGGCG AGCGGGGCCC AGGGGCCGGA CGGGGCCGGG 
GGTGGGCCCA CAGACACCGA CGAGGGGCTG CGGAAGGCGT CGGATCCCTC AGGAAGAGAG 
GATGCCGGGA GAAGAGCCAA CGTGGAGCCG GCTCAGTTTC AACCTCTTAT GGAAGAAGCG 
AGCCCCTGCC TCCCCAGCCC CCCGACGCTG GGCTCGTCCG CCCTAAACAG CTTGGTGGTC 
TCCTGCGCCT CGGCCGTGGG GGTCCACATC GCCGCCACCT ACGAAGCCGG CGCGCTGTCG 
CTCAGGAAGG TGATGAACTT TTCCGGCGAG GCGGGCTCGC CGTCGGGCCC CGAGGGGCCG 
AGAGACTGCC GGGAGGAGCA GGTCAAACTG GAATCGCTGC AGGGCAAGAA GAGCTCCAGC 
CTCGTGGACA TCAGAGAGGA GGAAGCCGAG GGCAGCAGTC GGAGGCTTTC CCTGCCCGGG 
TTGCTCTCCC AAGTCTCCCC CAGGCTCTTG AGGAAGGCGG CGCGGGCCAA GCCGCGGACG 
GTGGTGCTGA CCCCGACGTA CAGCGGCGAA GCGGACGCGC TGCTCCCGTC GCTGAGGACG 
GAGGTGTGGA GCTGGGGGAA GGGGAAGGAA GGCCAGCTGG GGCACGGGGA TGTCCTGCCC 
AGGCTTCAGC CCCTGTGTGT AAAATGCCTC GATGGTAAAG AAGTCATCCA CCTGGCCGCC 
GGGGACCACC ATTCCCTGGC CCTCACAGCC AAATCCCAGG TCTTCTCCTG GGGAAGCAAT 
ACCTTTGGGC AGCTGGGACA CTCGGATTCT CCAACCACAG TTCCTCGTCT TGCAAAGGTG 
TGCGGTGAAA ACCGCATCTG GAGTTTCGCC GCCGGCCAGG ATTTCTCCCT CTTCCTAGTG 
GACACGGAAG ACTTCCAGCC TGGATTGTAT TACAGCGGCC GGCAGGACTC TCCAGAAGGT 
GACGGTGGCC CAGAGAACGC CGTCGCCACA AAGTCTCCCA GGCTGCTGAC GTCTTGTAGC 
AAGCTGGGCT ACATCAGCAG TGTGACAGCC GGCAGGGACA GCTGTCTCGC CCTGGTGGAC 
AAAAACATCA TGGGCTACAT CGCCAGCCTG CATGAGCTGG CTGCTACGGA AAGACGATTC 
TATTCCATGC TGAGCACCGT CAAATCCCAG GTTCTCCGAC CTCTTCTCAG TTTAGATAAC 
TTGGGCGCCC CAGCTTCGGT CCAGCTCCTT CAAGAGGTGG CCAGCAGGTT CAGCAAGCTG 
TGTTACCTGA TTGGGCAGCA CGCGGCCTCC TTGAGCATCT TCCTCCGCGG GATAAAAGAA 
GTGCGGAGCC TGACGGTCCT GAAGCATGCA AGTCTCTTCT TGGATAGTTA CACAGAGTAC 
TGCACGGCCC TGACCAATTT TATCGTCATG GGAGGATTCC AGCTCCTCGC CAAACCGGCC 
ATGGACTTCT TAAATAAAAA CCAGGAGCTG TTACAGGAGC TGTCGGAGAC GAAAGACGGG 
GACGTCCAGC TGTTGGAGAT GCTGAATGCC TTATTCTTCC TCCCAATCCG GCGGCTTCAT 
GATTACGCCC GAGCTCTGCT AAAGCTGGCT ACATGTTTTG AGGTGGCCTC TCCGGAGTAC 
CAGACGCTGC ACGATTCCAG TTCCTGCTAC GAGACCCTCG CTCTCCACCT TGGCAGGAAA 
AGGAAGGAAG CAGAAGCCAC GCTGAGCTTC TGGAAGACGT TCCCCGGAAA GATGACGGAC 
TCCTTGAGGA AGCCAGAGCG CAGGCTAATC TGCGAGAGCA GCAATCGAGC GCTGGCCCTG 
CAGCATGCTG GGAGATTCTC CGTGAATTGG TTCATTCTCT TTAACGATGC CTTGGTCCAT 
GCCCAGTTCT CGACCCATCA CATCTTCCCC CTGTCCACTC TGTGGGTCGA GCCTATCTCA 
GAAGAAGGCA ACAGCGCGAA CGGCCTGAAG TTGACGACGC CGGAGGAACA GTTCACCCTC 
ATTTCCTCGA CCCCGCAGGA GAAGACCAAG TGGCTGCGGG CCCTGAGCCA GGCGGTGGAC 
CAGGCCCTGA GGGGCGCGGC CGACTTCCCC CCCTACGGCA GCGGCGCCCC GCGGCCGGAC 
CCCCCCGCCT CCCGCAGCGC CAGCTACACC TTCTACAAGG CCTCCCGCCT CAAGGAAGCC 
ACCTACGAGG GCCGCTGGCT CTCCGGGAAG CCCCACGGCA GAGGGGTCTT GAAGTGGACC 
GACGGCAAGA CGTACTCCGG TATGTTTCGG AATGGCCTGG AAGATGGATA CGGGGAATAT 
AAAGTCCCAA ACAAAGCACT TAACAAAGAA GACCACTACG TCGGGTACTG GAAAGAAGGA 
AAAATGTGCG GACAAGGAGT TTACAGCTAC GCCACGGGCG AAGTGTACGA AGGCTACTTC 
CAAGAGAACG TCCGCCACGG CCACGGGCTT CTCCGCAGCG GCAAGCTGAC CTCCACCTCC 
CCCAGCATGT TCATCGGCCA GTGGACCATG GACAAGAAGG CCGGATACGG CGTCTTCGAC 
GACATCACCA GGGGAGAAAA GTACATGGGC ATGTGGCAAG ATGACATGTG TGAGGGCAAC 
GGCGTGGTGG TCACTCAGTT CGGACTCTAT TACGAAGGCG CCTTTAGCCT TAACAAAATG 
ATGGGAACTG GAATTTTGCT CTCTGAAGAT GATACAGTCT ACGAGGGAGA ATTTTCAGAT 
GACTGGACCC TCAATGGAAA GGGGACGCTG ACCCTGCTGA ATGGAGATTA CATCGAAGGC 
CATTTTAGCG GAGAATGGGG GTCTGGGATC AAAATCACCG GAACCTACTT CAAACCCAGC 
CTGTACGAGA GCGATAAGGA GCGGCCCAAG GCCGTGAAGC TCGGAAACCT GGCCGTGGCG 
GCGGACGAGA AGTGGACGGC GGTGTTCGAG GAGTGCTGGC GACAGCTGGG CTGCGAGTCT 
CCCGGGCAGG GGGAGAGCTG GAAGGCCTGG GATAACATCG CCGTGGCCCT GACCGCCAAC 
AGACGCCAGC ACCGAGACAG CCCCGAGATC CTAAGTCGTT CGCACACTCA GACGCTGGAG 
AGCCTGGAAT TCATCCCGCA GCACGGCGGG GACTTCACGG TGGAGAAATA CGAAGACATC 
AAGAGGTATC TGATGAAGGC CTGCGACACC CCTCTGCACC CTCTGGGGCA GCTGGTAGAG 
ACCCTGGTGG CAGTGTACAG GATGACTTAC GTGGGCGTGG GAGCCAACCG GCGGTTACTA 
CAGGAGGCCG TCAGAGAGAT CCAGTCCTAC CTGAAGCGCA TCTTCCAGCT TGTCAGGTTC 
CTTTTTCCCG ACCTTCCCGA AGAAGGCAGC ACGATTCCAC TCTCCTCCAG TGTCCCACCG 
GAGAGGAAAT CCTTCTGCAC CGGAAAAGCC GATTCCAGAT CTGAATCTCC AGAGCCGGGC 
TACGTGGTGA CGAGCTCCGG GCTGCTGCTT CCCGTGCTGC TGCCTCGGCT CTACCCGCCG 
CTGTTCACGC TGTACGCCCT GGACAACGAG CGGGAGGAAG ACGCCTACTG GGAGTGCGTC 
CTGCGCCTCA ACAAGCAGCC CGACACGGCG CTCCTCAGCT TCCTGGGAGT GCAGCGGAAG 
TTTTGGCCTG ACAACGTGTC GCTCCTCGGA GACAGCAGAA AGGTGCTGCC GACCACGAAG 
GAGGCCTGTT TCGCCTCCGC GGTGGAGTGT CTGCAGCAGA TCAGCACGAC CTTCACTCCG 
TCGGACAAGT TGAAGGTGAT CCAGCAGACC TTCGAGGAGA TCTCCCAGAG CGTCCTGGCG 
TGGCTCCGAG AAGACTTCCT GTGGTCCATG GACGACCTGT TTCCCGTGTT CCTCTACGTG 
GTTCTCCGGG CCAGAATCCG CAATTTGGGC TCGGAAGTCC ACCTGATCGA GGACCTGATG 
GACCCCTACC TGCAGCACGG CGAGCAGGGC ATCATGTTCA CCACCCTGAA GGCCTGTTAC 
TACCAGATCC AGCGAGAGAA ACTGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_029068797.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028924630.1 Ornithorhynchus anatinus alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X3, mRNA.

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CloneID	OOc131912
Clone ID Related Accession (Same CDS sequence)	XM_029068795.1 , XM_029068796.1 , XM_029068797.1
Accession Version	XM_029068797.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556380800000
Organism	Ornithorhynchus anatinus(platypus)
Product	alsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCGC AGGAGAGCAG CTCAGCCGAG GCAGAAGGAG CCAGAGAGAG GGGCCTGGTC 
CGCGTTTGGT CGGCGAGGTC GTGCCGTGAG ACCCCAGAGA GGCTTCGGGG CTGGGGAGGG 
AAGACCGTCC TCCAAGCGGC CCTGGGCGTC AGTCACGGCG TCCTCCTGAC CGAAGGCGGC 
GAAGTGTACA GCTTCGGACA GCTGCCCTGG AAGCTCGAGG CGGAGGCGGA GGGACCGCGG 
CCGAGCGGCC CCGTCCTAGA ACATGCCCTG GTCGGGCAGC GCGTGGTGGC CGTGGCCACG 
GGCAGCTTCC ACAGCGGGGC CGTCACGGAG GCCGGCGCCG TGTACATGTG GGGGGAGAAC 
GGCGCGGGCC AGTGCGCCGT GGCCGAGCAG GCCCGCGTGC CCGAGCCCAG TCTCGTGGCC 
CTGGAGGACC CCGAGCCCGG CCCGCCGCTG CCCGTGCGCG TCCTGCAGCT GGCCTGCGGC 
GAGGAGCACA CCCTGGCCCT GTCCATCAGC CGGGAGATCT GGGCCTGGGG CACGGGCTGC 
CAGCTGGGCC TCCTGACGGC CTCCTTCCCG GTGACCAGGC CGCAGAAGGT GGAGCACCTG 
GCCGGGCGGG TGGTGCTGCA GGTGGCCTGC GGGCTCTCCC ACAGCCTGGC CCTGGTCCAG 
TGCCCGCCCG CCCTCGACCG GAAGCCGCTC CCCGAGAGAT GCCGCCACTG CAGCCAGCTC 
CTGATCACCA TGACGGACAA AGAAGACCAC GTCATCATAT CGGACAGTCA CTGCTGCCCT 
CTGGGAGTGG TCCTCCCCGA CGTCCAGGAG GACGGCGGCA CCGGGCCCAA GCCCCCGCTG 
GGGCCCCCGG ACGGGGGAGG CCCGGCCGAG GCCCCGGGCC AGGGCCGGGG AAGGGGCCCA 
GTGGGGGCCC ACGCGCCTGT GGCTCCCGAA GGGCCCGTCG GCCCGGCCCA GGGTGTGGGA 
GGGGAGGCCG AACCCTGCCC GCCGGAGGCG AGCGGGGCCC AGGGGCCGGA CGGGGCCGGG 
GGTGGGCCCA CAGACACCGA CGAGGGGCTG CGGAAGGCGT CGGATCCCTC AGGAAGAGAG 
GATGCCGGGA GAAGAGCCAA CGTGGAGCCG GCTCAGTTTC AACCTCTTAT GGAAGAAGCG 
AGCCCCTGCC TCCCCAGCCC CCCGACGCTG GGCTCGTCCG CCCTAAACAG CTTGGTGGTC 
TCCTGCGCCT CGGCCGTGGG GGTCCACATC GCCGCCACCT ACGAAGCCGG CGCGCTGTCG 
CTCAGGAAGG TGATGAACTT TTCCGGCGAG GCGGGCTCGC CGTCGGGCCC CGAGGGGCCG 
AGAGACTGCC GGGAGGAGCA GGTCAAACTG GAATCGCTGC AGGGCAAGAA GAGCTCCAGC 
CTCGTGGACA TCAGAGAGGA GGAAGCCGAG GGCAGCAGTC GGAGGCTTTC CCTGCCCGGG 
TTGCTCTCCC AAGTCTCCCC CAGGCTCTTG AGGAAGGCGG CGCGGGCCAA GCCGCGGACG 
GTGGTGCTGA CCCCGACGTA CAGCGGCGAA GCGGACGCGC TGCTCCCGTC GCTGAGGACG 
GAGGTGTGGA GCTGGGGGAA GGGGAAGGAA GGCCAGCTGG GGCACGGGGA TGTCCTGCCC 
AGGCTTCAGC CCCTGTGTGT AAAATGCCTC GATGGTAAAG AAGTCATCCA CCTGGCCGCC 
GGGGACCACC ATTCCCTGGC CCTCACAGCC AAATCCCAGG TCTTCTCCTG GGGAAGCAAT 
ACCTTTGGGC AGCTGGGACA CTCGGATTCT CCAACCACAG TTCCTCGTCT TGCAAAGGTG 
TGCGGTGAAA ACCGCATCTG GAGTTTCGCC GCCGGCCAGG ATTTCTCCCT CTTCCTAGTG 
GACACGGAAG ACTTCCAGCC TGGATTGTAT TACAGCGGCC GGCAGGACTC TCCAGAAGGT 
GACGGTGGCC CAGAGAACGC CGTCGCCACA AAGTCTCCCA GGCTGCTGAC GTCTTGTAGC 
AAGCTGGGCT ACATCAGCAG TGTGACAGCC GGCAGGGACA GCTGTCTCGC CCTGGTGGAC 
AAAAACATCA TGGGCTACAT CGCCAGCCTG CATGAGCTGG CTGCTACGGA AAGACGATTC 
TATTCCATGC TGAGCACCGT CAAATCCCAG GTTCTCCGAC CTCTTCTCAG TTTAGATAAC 
TTGGGCGCCC CAGCTTCGGT CCAGCTCCTT CAAGAGGTGG CCAGCAGGTT CAGCAAGCTG 
TGTTACCTGA TTGGGCAGCA CGCGGCCTCC TTGAGCATCT TCCTCCGCGG GATAAAAGAA 
GTGCGGAGCC TGACGGTCCT GAAGCATGCA AGTCTCTTCT TGGATAGTTA CACAGAGTAC 
TGCACGGCCC TGACCAATTT TATCGTCATG GGAGGATTCC AGCTCCTCGC CAAACCGGCC 
ATGGACTTCT TAAATAAAAA CCAGGAGCTG TTACAGGAGC TGTCGGAGAC GAAAGACGGG 
GACGTCCAGC TGTTGGAGAT GCTGAATGCC TTATTCTTCC TCCCAATCCG GCGGCTTCAT 
GATTACGCCC GAGCTCTGCT AAAGCTGGCT ACATGTTTTG AGGTGGCCTC TCCGGAGTAC 
CAGACGCTGC ACGATTCCAG TTCCTGCTAC GAGACCCTCG CTCTCCACCT TGGCAGGAAA 
AGGAAGGAAG CAGAAGCCAC GCTGAGCTTC TGGAAGACGT TCCCCGGAAA GATGACGGAC 
TCCTTGAGGA AGCCAGAGCG CAGGCTAATC TGCGAGAGCA GCAATCGAGC GCTGGCCCTG 
CAGCATGCTG GGAGATTCTC CGTGAATTGG TTCATTCTCT TTAACGATGC CTTGGTCCAT 
GCCCAGTTCT CGACCCATCA CATCTTCCCC CTGTCCACTC TGTGGGTCGA GCCTATCTCA 
GAAGAAGGCA ACAGCGCGAA CGGCCTGAAG TTGACGACGC CGGAGGAACA GTTCACCCTC 
ATTTCCTCGA CCCCGCAGGA GAAGACCAAG TGGCTGCGGG CCCTGAGCCA GGCGGTGGAC 
CAGGCCCTGA GGGGCGCGGC CGACTTCCCC CCCTACGGCA GCGGCGCCCC GCGGCCGGAC 
CCCCCCGCCT CCCGCAGCGC CAGCTACACC TTCTACAAGG CCTCCCGCCT CAAGGAAGCC 
ACCTACGAGG GCCGCTGGCT CTCCGGGAAG CCCCACGGCA GAGGGGTCTT GAAGTGGACC 
GACGGCAAGA CGTACTCCGG TATGTTTCGG AATGGCCTGG AAGATGGATA CGGGGAATAT 
AAAGTCCCAA ACAAAGCACT TAACAAAGAA GACCACTACG TCGGGTACTG GAAAGAAGGA 
AAAATGTGCG GACAAGGAGT TTACAGCTAC GCCACGGGCG AAGTGTACGA AGGCTACTTC 
CAAGAGAACG TCCGCCACGG CCACGGGCTT CTCCGCAGCG GCAAGCTGAC CTCCACCTCC 
CCCAGCATGT TCATCGGCCA GTGGACCATG GACAAGAAGG CCGGATACGG CGTCTTCGAC 
GACATCACCA GGGGAGAAAA GTACATGGGC ATGTGGCAAG ATGACATGTG TGAGGGCAAC 
GGCGTGGTGG TCACTCAGTT CGGACTCTAT TACGAAGGCG CCTTTAGCCT TAACAAAATG 
ATGGGAACTG GAATTTTGCT CTCTGAAGAT GATACAGTCT ACGAGGGAGA ATTTTCAGAT 
GACTGGACCC TCAATGGAAA GGGGACGCTG ACCCTGCTGA ATGGAGATTA CATCGAAGGC 
CATTTTAGCG GAGAATGGGG GTCTGGGATC AAAATCACCG GAACCTACTT CAAACCCAGC 
CTGTACGAGA GCGATAAGGA GCGGCCCAAG GCCGTGAAGC TCGGAAACCT GGCCGTGGCG 
GCGGACGAGA AGTGGACGGC GGTGTTCGAG GAGTGCTGGC GACAGCTGGG CTGCGAGTCT 
CCCGGGCAGG GGGAGAGCTG GAAGGCCTGG GATAACATCG CCGTGGCCCT GACCGCCAAC 
AGACGCCAGC ACCGAGACAG CCCCGAGATC CTAAGTCGTT CGCACACTCA GACGCTGGAG 
AGCCTGGAAT TCATCCCGCA GCACGGCGGG GACTTCACGG TGGAGAAATA CGAAGACATC 
AAGAGGTATC TGATGAAGGC CTGCGACACC CCTCTGCACC CTCTGGGGCA GCTGGTAGAG 
ACCCTGGTGG CAGTGTACAG GATGACTTAC GTGGGCGTGG GAGCCAACCG GCGGTTACTA 
CAGGAGGCCG TCAGAGAGAT CCAGTCCTAC CTGAAGCGCA TCTTCCAGCT TGTCAGGTTC 
CTTTTTCCCG ACCTTCCCGA AGAAGGCAGC ACGATTCCAC TCTCCTCCAG TGTCCCACCG 
GAGAGGAAAT CCTTCTGCAC CGGAAAAGCC GATTCCAGAT CTGAATCTCC AGAGCCGGGC 
TACGTGGTGA CGAGCTCCGG GCTGCTGCTT CCCGTGCTGC TGCCTCGGCT CTACCCGCCG 
CTGTTCACGC TGTACGCCCT GGACAACGAG CGGGAGGAAG ACGCCTACTG GGAGTGCGTC 
CTGCGCCTCA ACAAGCAGCC CGACACGGCG CTCCTCAGCT TCCTGGGAGT GCAGCGGAAG 
TTTTGGCCTG ACAACGTGTC GCTCCTCGGA GACAGCAGAA AGGTGCTGCC GACCACGAAG 
GAGGCCTGTT TCGCCTCCGC GGTGGAGTGT CTGCAGCAGA TCAGCACGAC CTTCACTCCG 
TCGGACAAGT TGAAGGTGAT CCAGCAGACC TTCGAGGAGA TCTCCCAGAG CGTCCTGGCG 
TGGCTCCGAG AAGACTTCCT GTGGTCCATG GACGACCTGT TTCCCGTGTT CCTCTACGTG 
GTTCTCCGGG CCAGAATCCG CAATTTGGGC TCGGAAGTCC ACCTGATCGA GGACCTGATG 
GACCCCTACC TGCAGCACGG CGAGCAGGGC ATCATGTTCA CCACCCTGAA GGCCTGTTAC 
TACCAGATCC AGCGAGAGAA ACTGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028924630.1
CDS	181..5130
Translation	MDPQESSSAEAEGARERGLVRVWSARSCRETPERLRGWGGKTVLQAALGVSHGVLLTEGGEVYSFGQLPWKLEAEAEGPRPSGPVLEHALVGQRVVAVATGSFHSGAVTEAGAVYMWGENGAGQCAVAEQARVPEPSLVALEDPEPGPPLPVRVLQLACGEEHTLALSISREIWAWGTGCQLGLLTASFPVTRPQKVEHLAGRVVLQVACGLSHSLALVQCPPALDRKPLPERCRHCSQLLITMTDKEDHVIISDSHCCPLGVVLPDVQEDGGTGPKPPLGPPDGGGPAEAPGQGRGRGPVGAHAPVAPEGPVGPAQGVGGEAEPCPPEASGAQGPDGAGGGPTDTDEGLRKASDPSGREDAGRRANVEPAQFQPLMEEASPCLPSPPTLGSSALNSLVVSCASAVGVHIAATYEAGALSLRKVMNFSGEAGSPSGPEGPRDCREEQVKLESLQGKKSSSLVDIREEEAEGSSRRLSLPGLLSQVSPRLLRKAARAKPRTVVLTPTYSGEADALLPSLRTEVWSWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLAAGDHHSLALTAKSQVFSWGSNTFGQLGHSDSPTTVPRLAKVCGENRIWSFAAGQDFSLFLVDTEDFQPGLYYSGRQDSPEGDGGPENAVATKSPRLLTSCSKLGYISSVTAGRDSCLALVDKNIMGYIASLHELAATERRFYSMLSTVKSQVLRPLLSLDNLGAPASVQLLQEVASRFSKLCYLIGQHAASLSIFLRGIKEVRSLTVLKHASLFLDSYTEYCTALTNFIVMGGFQLLAKPAMDFLNKNQELLQELSETKDGDVQLLEMLNALFFLPIRRLHDYARALLKLATCFEVASPEYQTLHDSSSCYETLALHLGRKRKEAEATLSFWKTFPGKMTDSLRKPERRLICESSNRALALQHAGRFSVNWFILFNDALVHAQFSTHHIFPLSTLWVEPISEEGNSANGLKLTTPEEQFTLISSTPQEKTKWLRALSQAVDQALRGAADFPPYGSGAPRPDPPASRSASYTFYKASRLKEATYEGRWLSGKPHGRGVLKWTDGKTYSGMFRNGLEDGYGEYKVPNKALNKEDHYVGYWKEGKMCGQGVYSYATGEVYEGYFQENVRHGHGLLRSGKLTSTSPSMFIGQWTMDKKAGYGVFDDITRGEKYMGMWQDDMCEGNGVVVTQFGLYYEGAFSLNKMMGTGILLSEDDTVYEGEFSDDWTLNGKGTLTLLNGDYIEGHFSGEWGSGIKITGTYFKPSLYESDKERPKAVKLGNLAVAADEKWTAVFEECWRQLGCESPGQGESWKAWDNIAVALTANRRQHRDSPEILSRSHTQTLESLEFIPQHGGDFTVEKYEDIKRYLMKACDTPLHPLGQLVETLVAVYRMTYVGVGANRRLLQEAVREIQSYLKRIFQLVRFLFPDLPEEGSTIPLSSSVPPERKSFCTGKADSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFTLYALDNEREEDAYWECVLRLNKQPDTALLSFLGVQRKFWPDNVSLLGDSRKVLPTTKEACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLAWLREDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_029068797.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029068797.1

ORF Insert Sequence:

ATGGACCCGC AGGAGAGCAG CTCAGCCGAG GCAGAAGGAG CCAGAGAGAG GGGCCTGGTC 
CGCGTTTGGT CGGCGAGGTC GTGCCGTGAG ACCCCAGAGA GGCTTCGGGG CTGGGGAGGG 
AAGACCGTCC TCCAAGCGGC CCTGGGCGTC AGTCACGGCG TCCTCCTGAC CGAAGGCGGC 
GAAGTGTACA GCTTCGGACA GCTGCCCTGG AAGCTCGAGG CGGAGGCGGA GGGACCGCGG 
CCGAGCGGCC CCGTCCTAGA ACATGCCCTG GTCGGGCAGC GCGTGGTGGC CGTGGCCACG 
GGCAGCTTCC ACAGCGGGGC CGTCACGGAG GCCGGCGCCG TGTACATGTG GGGGGAGAAC 
GGCGCGGGCC AGTGCGCCGT GGCCGAGCAG GCCCGCGTGC CCGAGCCCAG TCTCGTGGCC 
CTGGAGGACC CCGAGCCCGG CCCGCCGCTG CCCGTGCGCG TCCTGCAGCT GGCCTGCGGC 
GAGGAGCACA CCCTGGCCCT GTCCATCAGC CGGGAGATCT GGGCCTGGGG CACGGGCTGC 
CAGCTGGGCC TCCTGACGGC CTCCTTCCCG GTGACCAGGC CGCAGAAGGT GGAGCACCTG 
GCCGGGCGGG TGGTGCTGCA GGTGGCCTGC GGGCTCTCCC ACAGCCTGGC CCTGGTCCAG 
TGCCCGCCCG CCCTCGACCG GAAGCCGCTC CCCGAGAGAT GCCGCCACTG CAGCCAGCTC 
CTGATCACCA TGACGGACAA AGAAGACCAC GTCATCATAT CGGACAGTCA CTGCTGCCCT 
CTGGGAGTGG TCCTCCCCGA CGTCCAGGAG GACGGCGGCA CCGGGCCCAA GCCCCCGCTG 
GGGCCCCCGG ACGGGGGAGG CCCGGCCGAG GCCCCGGGCC AGGGCCGGGG AAGGGGCCCA 
GTGGGGGCCC ACGCGCCTGT GGCTCCCGAA GGGCCCGTCG GCCCGGCCCA GGGTGTGGGA 
GGGGAGGCCG AACCCTGCCC GCCGGAGGCG AGCGGGGCCC AGGGGCCGGA CGGGGCCGGG 
GGTGGGCCCA CAGACACCGA CGAGGGGCTG CGGAAGGCGT CGGATCCCTC AGGAAGAGAG 
GATGCCGGGA GAAGAGCCAA CGTGGAGCCG GCTCAGTTTC AACCTCTTAT GGAAGAAGCG 
AGCCCCTGCC TCCCCAGCCC CCCGACGCTG GGCTCGTCCG CCCTAAACAG CTTGGTGGTC 
TCCTGCGCCT CGGCCGTGGG GGTCCACATC GCCGCCACCT ACGAAGCCGG CGCGCTGTCG 
CTCAGGAAGG TGATGAACTT TTCCGGCGAG GCGGGCTCGC CGTCGGGCCC CGAGGGGCCG 
AGAGACTGCC GGGAGGAGCA GGTCAAACTG GAATCGCTGC AGGGCAAGAA GAGCTCCAGC 
CTCGTGGACA TCAGAGAGGA GGAAGCCGAG GGCAGCAGTC GGAGGCTTTC CCTGCCCGGG 
TTGCTCTCCC AAGTCTCCCC CAGGCTCTTG AGGAAGGCGG CGCGGGCCAA GCCGCGGACG 
GTGGTGCTGA CCCCGACGTA CAGCGGCGAA GCGGACGCGC TGCTCCCGTC GCTGAGGACG 
GAGGTGTGGA GCTGGGGGAA GGGGAAGGAA GGCCAGCTGG GGCACGGGGA TGTCCTGCCC 
AGGCTTCAGC CCCTGTGTGT AAAATGCCTC GATGGTAAAG AAGTCATCCA CCTGGCCGCC 
GGGGACCACC ATTCCCTGGC CCTCACAGCC AAATCCCAGG TCTTCTCCTG GGGAAGCAAT 
ACCTTTGGGC AGCTGGGACA CTCGGATTCT CCAACCACAG TTCCTCGTCT TGCAAAGGTG 
TGCGGTGAAA ACCGCATCTG GAGTTTCGCC GCCGGCCAGG ATTTCTCCCT CTTCCTAGTG 
GACACGGAAG ACTTCCAGCC TGGATTGTAT TACAGCGGCC GGCAGGACTC TCCAGAAGGT 
GACGGTGGCC CAGAGAACGC CGTCGCCACA AAGTCTCCCA GGCTGCTGAC GTCTTGTAGC 
AAGCTGGGCT ACATCAGCAG TGTGACAGCC GGCAGGGACA GCTGTCTCGC CCTGGTGGAC 
AAAAACATCA TGGGCTACAT CGCCAGCCTG CATGAGCTGG CTGCTACGGA AAGACGATTC 
TATTCCATGC TGAGCACCGT CAAATCCCAG GTTCTCCGAC CTCTTCTCAG TTTAGATAAC 
TTGGGCGCCC CAGCTTCGGT CCAGCTCCTT CAAGAGGTGG CCAGCAGGTT CAGCAAGCTG 
TGTTACCTGA TTGGGCAGCA CGCGGCCTCC TTGAGCATCT TCCTCCGCGG GATAAAAGAA 
GTGCGGAGCC TGACGGTCCT GAAGCATGCA AGTCTCTTCT TGGATAGTTA CACAGAGTAC 
TGCACGGCCC TGACCAATTT TATCGTCATG GGAGGATTCC AGCTCCTCGC CAAACCGGCC 
ATGGACTTCT TAAATAAAAA CCAGGAGCTG TTACAGGAGC TGTCGGAGAC GAAAGACGGG 
GACGTCCAGC TGTTGGAGAT GCTGAATGCC TTATTCTTCC TCCCAATCCG GCGGCTTCAT 
GATTACGCCC GAGCTCTGCT AAAGCTGGCT ACATGTTTTG AGGTGGCCTC TCCGGAGTAC 
CAGACGCTGC ACGATTCCAG TTCCTGCTAC GAGACCCTCG CTCTCCACCT TGGCAGGAAA 
AGGAAGGAAG CAGAAGCCAC GCTGAGCTTC TGGAAGACGT TCCCCGGAAA GATGACGGAC 
TCCTTGAGGA AGCCAGAGCG CAGGCTAATC TGCGAGAGCA GCAATCGAGC GCTGGCCCTG 
CAGCATGCTG GGAGATTCTC CGTGAATTGG TTCATTCTCT TTAACGATGC CTTGGTCCAT 
GCCCAGTTCT CGACCCATCA CATCTTCCCC CTGTCCACTC TGTGGGTCGA GCCTATCTCA 
GAAGAAGGCA ACAGCGCGAA CGGCCTGAAG TTGACGACGC CGGAGGAACA GTTCACCCTC 
ATTTCCTCGA CCCCGCAGGA GAAGACCAAG TGGCTGCGGG CCCTGAGCCA GGCGGTGGAC 
CAGGCCCTGA GGGGCGCGGC CGACTTCCCC CCCTACGGCA GCGGCGCCCC GCGGCCGGAC 
CCCCCCGCCT CCCGCAGCGC CAGCTACACC TTCTACAAGG CCTCCCGCCT CAAGGAAGCC 
ACCTACGAGG GCCGCTGGCT CTCCGGGAAG CCCCACGGCA GAGGGGTCTT GAAGTGGACC 
GACGGCAAGA CGTACTCCGG TATGTTTCGG AATGGCCTGG AAGATGGATA CGGGGAATAT 
AAAGTCCCAA ACAAAGCACT TAACAAAGAA GACCACTACG TCGGGTACTG GAAAGAAGGA 
AAAATGTGCG GACAAGGAGT TTACAGCTAC GCCACGGGCG AAGTGTACGA AGGCTACTTC 
CAAGAGAACG TCCGCCACGG CCACGGGCTT CTCCGCAGCG GCAAGCTGAC CTCCACCTCC 
CCCAGCATGT TCATCGGCCA GTGGACCATG GACAAGAAGG CCGGATACGG CGTCTTCGAC 
GACATCACCA GGGGAGAAAA GTACATGGGC ATGTGGCAAG ATGACATGTG TGAGGGCAAC 
GGCGTGGTGG TCACTCAGTT CGGACTCTAT TACGAAGGCG CCTTTAGCCT TAACAAAATG 
ATGGGAACTG GAATTTTGCT CTCTGAAGAT GATACAGTCT ACGAGGGAGA ATTTTCAGAT 
GACTGGACCC TCAATGGAAA GGGGACGCTG ACCCTGCTGA ATGGAGATTA CATCGAAGGC 
CATTTTAGCG GAGAATGGGG GTCTGGGATC AAAATCACCG GAACCTACTT CAAACCCAGC 
CTGTACGAGA GCGATAAGGA GCGGCCCAAG GCCGTGAAGC TCGGAAACCT GGCCGTGGCG 
GCGGACGAGA AGTGGACGGC GGTGTTCGAG GAGTGCTGGC GACAGCTGGG CTGCGAGTCT 
CCCGGGCAGG GGGAGAGCTG GAAGGCCTGG GATAACATCG CCGTGGCCCT GACCGCCAAC 
AGACGCCAGC ACCGAGACAG CCCCGAGATC CTAAGTCGTT CGCACACTCA GACGCTGGAG 
AGCCTGGAAT TCATCCCGCA GCACGGCGGG GACTTCACGG TGGAGAAATA CGAAGACATC 
AAGAGGTATC TGATGAAGGC CTGCGACACC CCTCTGCACC CTCTGGGGCA GCTGGTAGAG 
ACCCTGGTGG CAGTGTACAG GATGACTTAC GTGGGCGTGG GAGCCAACCG GCGGTTACTA 
CAGGAGGCCG TCAGAGAGAT CCAGTCCTAC CTGAAGCGCA TCTTCCAGCT TGTCAGGTTC 
CTTTTTCCCG ACCTTCCCGA AGAAGGCAGC ACGATTCCAC TCTCCTCCAG TGTCCCACCG 
GAGAGGAAAT CCTTCTGCAC CGGAAAAGCC GATTCCAGAT CTGAATCTCC AGAGCCGGGC 
TACGTGGTGA CGAGCTCCGG GCTGCTGCTT CCCGTGCTGC TGCCTCGGCT CTACCCGCCG 
CTGTTCACGC TGTACGCCCT GGACAACGAG CGGGAGGAAG ACGCCTACTG GGAGTGCGTC 
CTGCGCCTCA ACAAGCAGCC CGACACGGCG CTCCTCAGCT TCCTGGGAGT GCAGCGGAAG 
TTTTGGCCTG ACAACGTGTC GCTCCTCGGA GACAGCAGAA AGGTGCTGCC GACCACGAAG 
GAGGCCTGTT TCGCCTCCGC GGTGGAGTGT CTGCAGCAGA TCAGCACGAC CTTCACTCCG 
TCGGACAAGT TGAAGGTGAT CCAGCAGACC TTCGAGGAGA TCTCCCAGAG CGTCCTGGCG 
TGGCTCCGAG AAGACTTCCT GTGGTCCATG GACGACCTGT TTCCCGTGTT CCTCTACGTG 
GTTCTCCGGG CCAGAATCCG CAATTTGGGC TCGGAAGTCC ACCTGATCGA GGACCTGATG 
GACCCCTACC TGCAGCACGG CGAGCAGGGC ATCATGTTCA CCACCCTGAA GGCCTGTTAC 
TACCAGATCC AGCGAGAGAA ACTGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALS2 ( XM_029068798.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028924631.1 Ornithorhynchus anatinus alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X4, mRNA.

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CloneID	OOc131913
Clone ID Related Accession (Same CDS sequence)	XM_029068798.1
Accession Version	XM_029068798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556380800000
Organism	Ornithorhynchus anatinus(platypus)
Product	alsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041734.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCGC AGGAGAGCAG CTCAGCCGAG GCAGAAGGAG CCAGAGAGAG GGGCCTGGTC 
CGCGTTTGGT CGGCGAGGTC GTGCCGTGAG ACCCCAGAGA GGCTTCGGGG CTGGGGAGGG 
AAGACCGTCC TCCAAGCGGC CCTGGGCGTC AGTCACGGCG TCCTCCTGAC CGAAGGCGGC 
GAAGTGTACA GCTTCGGACA GCTGCCCTGG AAGCTCGAGG CGGAGGCGGA GGGACCGCGG 
CCGAGCGGCC CCGTCCTAGA ACATGCCCTG GTCGGGCAGC GCGTGGTGGC CGTGGCCACG 
GGCAGCTTCC ACAGCGGGGC CGTCACGGAG GCCGGCGCCG TGTACATGTG GGGGGAGAAC 
GGCGCGGGCC AGTGCGCCGT GGCCGAGCAG GCCCGCGTGC CCGAGCCCAG TCTCGTGGCC 
CTGGAGGACC CCGAGCCCGG CCCGCCGCTG CCCGTGCGCG TCCTGCAGCT GGCCTGCGGC 
GAGGAGCACA CCCTGGCCCT GTCCATCAGC CGGGAGATCT GGGCCTGGGG CACGGGCTGC 
CAGCTGGGCC TCCTGACGGC CTCCTTCCCG GTGACCAGGC CGCAGAAGGT GGAGCACCTG 
GCCGGGCGGG TGGTGCTGCA GGTGGCCTGC GGGCTCTCCC ACAGCCTGGC CCTGGTCCAG 
TGCCCGCCCG CCCTCGACCG GAAGCCGCTC CCCGAGAGAT GCCGCCACTG CAGCCAGCTC 
CTGATCACCA TGACGGACAA AGAAGACCAC GTCATCATAT CGGACAGTCA CTGCTGCCCT 
CTGGGAGTGG TCCTCCCCGA CGTCCAGGAG GACGGCGGCA CCGGGCCCAA GCCCCCGCTG 
GGGCCCCCGG ACGGGGGAGG CCCGGCCGAG GCCCCGGGCC AGGGCCGGGG AAGGGGCCCA 
GTGGGGGCCC ACGCGCCTGT GGCTCCCGAA GGGCCCGTCG GCCCGGCCCA GGGTGTGGGA 
GGGGAGGCCG AACCCTGCCC GCCGGAGGCG AGCGGGGCCC AGGGGCCGGA CGGGGCCGGG 
GGTGGGCCCA CAGACACCGA CGAGGGGCTG CGGAAGGCGT CGGATCCCTC AGGAAGAGAG 
GATGCCGGGA GAAGAGCCAA CGTGGAGCCG GCTCAGTTTC AACCTCTTAT GGAAGAAGCG 
AGCCCCTGCC TCCCCAGCCC CCCGACGCTG GGCTCGTCCG CCCTAAACAG CTTGGTGGTC 
TCCTGCGCCT CGGCCGTGGG GGTCCACATC GCCGCCACCT ACGAAGCCGG CGCGCTGTCG 
CTCAGGAAGG TGATGAACTT TTCCGGCGAG GCGGGCTCGC CGTCGGGCCC CGAGGGGCCG 
AGAGACTGCC GGGAGGAGCA GGTCAAACTG GAATCGCTGC AGGGCAAGAA GAGCTCCAGC 
CTCGTGGACA TCAGAGAGGA GGAAGCCGAG GGCAGCAGTC GGAGGCTTTC CCTGCCCGGG 
TTGCTCTCCC AAGTCTCCCC CAGGCTCTTG AGGAAGGCGG CGCGGGCCAA GCCGCGGACG 
GTGGTGCTGA CCCCGACGTA CAGCGGCGAA GCGGACGCGC TGCTCCCGTC GCTGAGGACG 
GAGGTGTGGA GCTGGGGGAA GGGGAAGGAA GGCCAGCTGG GGCACGGGGA TGTCCTGCCC 
AGGCTTCAGC CCCTGTGTGT AAAATGCCTC GATGGTAAAG AAGTCATCCA CCTGGCCGCC 
GGGGACCACC ATTCCCTGGC CCTCACAGCC AAATCCCAGG TCTTCTCCTG GGGAAGCAAT 
ACCTTTGGGC AGCTGGGACA CTCGGATTCT CCAACCACAG TTCCTCGTCT TGCAAAGGTG 
TGCGGTGAAA ACCGCATCTG GAGTTTCGCC GCCGGCCAGG ATTTCTCCCT CTTCCTAGTG 
GACACGGAAG ACTTCCAGCC TGGATTGTAT TACAGCGGCC GGCAGGACTC TCCAGAAGGT 
GACGGTGGCC CAGAGAACGC CGTCGCCACA AAGTCTCCCA GGCTGCTGAC GTCTTGTAGC 
AAGCTGGGCT ACATCAGCAG TGTGACAGCC GGCAGGGACA GCTGTCTCGC CCTGGTGGAC 
AAAAACATCA TGGGCTACAT CGCCAGCCTG CATGAGCTGG CTGCTACGGA AAGACGATTC 
TATTCCATGC TGAGCACCGT CAAATCCCAG GTTCTCCGAC CTCTTCTCAG TTTAGATAAC 
TTGGGCGCCC CAGCTTCGGT CCAGCTCCTT CAAGAGGTGG CCAGCAGGTT CAGCAAGCTG 
TGTTACCTGA TTGGGCAGCA CGCGGCCTCC TTGAGCATCT TCCTCCGCGG GATAAAAGAA 
GTGCGGAGCC TGACGGTCCT GAAGCATGCA AGTCTCTTCT TGGATAGTTA CACAGAGTAC 
TGCACGGCCC TGACCAATTT TATCGTCATG GGAGGATTCC AGCTCCTCGC CAAACCGGCC 
ATGGACTTCT TAAATAAAAA CCAGGAGCTG TTACAGGAGC TGTCGGAGAC GAAAGACGGG 
GACGTCCAGC TGTTGGAGAT GCTGAATGCC TTATTCTTCC TCCCAATCCG GCGGCTTCAT 
GATTACGCCC GAGCTCTGCT AAAGCTGGCT ACATGTTTTG AGGCCTCTCC GGAGTACCAG 
ACGCTGCACG ATTCCAGTTC CTGCTACGAG ACCCTCGCTC TCCACCTTGG CAGGAAAAGG 
AAGGAAGCAG AAGCCACGCT GAGCTTCTGG AAGACGTTCC CCGGAAAGAT GACGGACTCC 
TTGAGGAAGC CAGAGCGCAG GCTAATCTGC GAGAGCAGCA ATCGAGCGCT GGCCCTGCAG 
CATGCTGGGA GATTCTCCGT GAATTGGTTC ATTCTCTTTA ACGATGCCTT GGTCCATGCC 
CAGTTCTCGA CCCATCACAT CTTCCCCCTG TCCACTCTGT GGGTCGAGCC TATCTCAGAA 
GAAGGCAACA GCGCGAACGG CCTGAAGTTG ACGACGCCGG AGGAACAGTT CACCCTCATT 
TCCTCGACCC CGCAGGAGAA GACCAAGTGG CTGCGGGCCC TGAGCCAGGC GGTGGACCAG 
GCCCTGAGGG GCGCGGCCGA CTTCCCCCCC TACGGCAGCG GCGCCCCGCG GCCGGACCCC 
CCCGCCTCCC GCAGCGCCAG CTACACCTTC TACAAGGCCT CCCGCCTCAA GGAAGCCACC 
TACGAGGGCC GCTGGCTCTC CGGGAAGCCC CACGGCAGAG GGGTCTTGAA GTGGACCGAC 
GGCAAGACGT ACTCCGGTAT GTTTCGGAAT GGCCTGGAAG ATGGATACGG GGAATATAAA 
GTCCCAAACA AAGCACTTAA CAAAGAAGAC CACTACGTCG GGTACTGGAA AGAAGGAAAA 
ATGTGCGGAC AAGGAGTTTA CAGCTACGCC ACGGGCGAAG TGTACGAAGG CTACTTCCAA 
GAGAACGTCC GCCACGGCCA CGGGCTTCTC CGCAGCGGCA AGCTGACCTC CACCTCCCCC 
AGCATGTTCA TCGGCCAGTG GACCATGGAC AAGAAGGCCG GATACGGCGT CTTCGACGAC 
ATCACCAGGG GAGAAAAGTA CATGGGCATG TGGCAAGATG ACATGTGTGA GGGCAACGGC 
GTGGTGGTCA CTCAGTTCGG ACTCTATTAC GAAGGCGCCT TTAGCCTTAA CAAAATGATG 
GGAACTGGAA TTTTGCTCTC TGAAGATGAT ACAGTCTACG AGGGAGAATT TTCAGATGAC 
TGGACCCTCA ATGGAAAGGG GACGCTGACC CTGCTGAATG GAGATTACAT CGAAGGCCAT 
TTTAGCGGAG AATGGGGGTC TGGGATCAAA ATCACCGGAA CCTACTTCAA ACCCAGCCTG 
TACGAGAGCG ATAAGGAGCG GCCCAAGGCC GTGAAGCTCG GAAACCTGGC CGTGGCGGCG 
GACGAGAAGT GGACGGCGGT GTTCGAGGAG TGCTGGCGAC AGCTGGGCTG CGAGTCTCCC 
GGGCAGGGGG AGAGCTGGAA GGCCTGGGAT AACATCGCCG TGGCCCTGAC CGCCAACAGA 
CGCCAGCACC GAGACAGCCC CGAGATCCTA AGTCGTTCGC ACACTCAGAC GCTGGAGAGC 
CTGGAATTCA TCCCGCAGCA CGGCGGGGAC TTCACGGTGG AGAAATACGA AGACATCAAG 
AGGTATCTGA TGAAGGCCTG CGACACCCCT CTGCACCCTC TGGGGCAGCT GGTAGAGACC 
CTGGTGGCAG TGTACAGGAT GACTTACGTG GGCGTGGGAG CCAACCGGCG GTTACTACAG 
GAGGCCGTCA GAGAGATCCA GTCCTACCTG AAGCGCATCT TCCAGCTTGT CAGGTTCCTT 
TTTCCCGACC TTCCCGAAGA AGGCAGCACG ATTCCACTCT CCTCCAGTGT CCCACCGGAG 
AGGAAATCCT TCTGCACCGG AAAAGCCGAT TCCAGATCTG AATCTCCAGA GCCGGGCTAC 
GTGGTGACGA GCTCCGGGCT GCTGCTTCCC GTGCTGCTGC CTCGGCTCTA CCCGCCGCTG 
TTCACGCTGT ACGCCCTGGA CAACGAGCGG GAGGAAGACG CCTACTGGGA GTGCGTCCTG 
CGCCTCAACA AGCAGCCCGA CACGGCGCTC CTCAGCTTCC TGGGAGTGCA GCGGAAGTTT 
TGGCCTGACA ACGTGTCGCT CCTCGGAGAC AGCAGAAAGG TGCTGCCGAC CACGAAGGAG 
GCCTGTTTCG CCTCCGCGGT GGAGTGTCTG CAGCAGATCA GCACGACCTT CACTCCGTCG 
GACAAGTTGA AGGTGATCCA GCAGACCTTC GAGGAGATCT CCCAGAGCGT CCTGGCGTGG 
CTCCGAGAAG ACTTCCTGTG GTCCATGGAC GACCTGTTTC CCGTGTTCCT CTACGTGGTT 
CTCCGGGCCA GAATCCGCAA TTTGGGCTCG GAAGTCCACC TGATCGAGGA CCTGATGGAC 
CCCTACCTGC AGCACGGCGA GCAGGGCATC ATGTTCACCA CCCTGAAGGC CTGTTACTAC 
CAGATCCAGC GAGAGAAACT GAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028924631.1
CDS	324..5270
Translation	MDPQESSSAEAEGARERGLVRVWSARSCRETPERLRGWGGKTVLQAALGVSHGVLLTEGGEVYSFGQLPWKLEAEAEGPRPSGPVLEHALVGQRVVAVATGSFHSGAVTEAGAVYMWGENGAGQCAVAEQARVPEPSLVALEDPEPGPPLPVRVLQLACGEEHTLALSISREIWAWGTGCQLGLLTASFPVTRPQKVEHLAGRVVLQVACGLSHSLALVQCPPALDRKPLPERCRHCSQLLITMTDKEDHVIISDSHCCPLGVVLPDVQEDGGTGPKPPLGPPDGGGPAEAPGQGRGRGPVGAHAPVAPEGPVGPAQGVGGEAEPCPPEASGAQGPDGAGGGPTDTDEGLRKASDPSGREDAGRRANVEPAQFQPLMEEASPCLPSPPTLGSSALNSLVVSCASAVGVHIAATYEAGALSLRKVMNFSGEAGSPSGPEGPRDCREEQVKLESLQGKKSSSLVDIREEEAEGSSRRLSLPGLLSQVSPRLLRKAARAKPRTVVLTPTYSGEADALLPSLRTEVWSWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLAAGDHHSLALTAKSQVFSWGSNTFGQLGHSDSPTTVPRLAKVCGENRIWSFAAGQDFSLFLVDTEDFQPGLYYSGRQDSPEGDGGPENAVATKSPRLLTSCSKLGYISSVTAGRDSCLALVDKNIMGYIASLHELAATERRFYSMLSTVKSQVLRPLLSLDNLGAPASVQLLQEVASRFSKLCYLIGQHAASLSIFLRGIKEVRSLTVLKHASLFLDSYTEYCTALTNFIVMGGFQLLAKPAMDFLNKNQELLQELSETKDGDVQLLEMLNALFFLPIRRLHDYARALLKLATCFEASPEYQTLHDSSSCYETLALHLGRKRKEAEATLSFWKTFPGKMTDSLRKPERRLICESSNRALALQHAGRFSVNWFILFNDALVHAQFSTHHIFPLSTLWVEPISEEGNSANGLKLTTPEEQFTLISSTPQEKTKWLRALSQAVDQALRGAADFPPYGSGAPRPDPPASRSASYTFYKASRLKEATYEGRWLSGKPHGRGVLKWTDGKTYSGMFRNGLEDGYGEYKVPNKALNKEDHYVGYWKEGKMCGQGVYSYATGEVYEGYFQENVRHGHGLLRSGKLTSTSPSMFIGQWTMDKKAGYGVFDDITRGEKYMGMWQDDMCEGNGVVVTQFGLYYEGAFSLNKMMGTGILLSEDDTVYEGEFSDDWTLNGKGTLTLLNGDYIEGHFSGEWGSGIKITGTYFKPSLYESDKERPKAVKLGNLAVAADEKWTAVFEECWRQLGCESPGQGESWKAWDNIAVALTANRRQHRDSPEILSRSHTQTLESLEFIPQHGGDFTVEKYEDIKRYLMKACDTPLHPLGQLVETLVAVYRMTYVGVGANRRLLQEAVREIQSYLKRIFQLVRFLFPDLPEEGSTIPLSSSVPPERKSFCTGKADSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFTLYALDNEREEDAYWECVLRLNKQPDTALLSFLGVQRKFWPDNVSLLGDSRKVLPTTKEACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLAWLREDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN

Target ORF information:

RefSeq Version	XM_029068798.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029068798.1

ORF Insert Sequence:

ATGGACCCGC AGGAGAGCAG CTCAGCCGAG GCAGAAGGAG CCAGAGAGAG GGGCCTGGTC 
CGCGTTTGGT CGGCGAGGTC GTGCCGTGAG ACCCCAGAGA GGCTTCGGGG CTGGGGAGGG 
AAGACCGTCC TCCAAGCGGC CCTGGGCGTC AGTCACGGCG TCCTCCTGAC CGAAGGCGGC 
GAAGTGTACA GCTTCGGACA GCTGCCCTGG AAGCTCGAGG CGGAGGCGGA GGGACCGCGG 
CCGAGCGGCC CCGTCCTAGA ACATGCCCTG GTCGGGCAGC GCGTGGTGGC CGTGGCCACG 
GGCAGCTTCC ACAGCGGGGC CGTCACGGAG GCCGGCGCCG TGTACATGTG GGGGGAGAAC 
GGCGCGGGCC AGTGCGCCGT GGCCGAGCAG GCCCGCGTGC CCGAGCCCAG TCTCGTGGCC 
CTGGAGGACC CCGAGCCCGG CCCGCCGCTG CCCGTGCGCG TCCTGCAGCT GGCCTGCGGC 
GAGGAGCACA CCCTGGCCCT GTCCATCAGC CGGGAGATCT GGGCCTGGGG CACGGGCTGC 
CAGCTGGGCC TCCTGACGGC CTCCTTCCCG GTGACCAGGC CGCAGAAGGT GGAGCACCTG 
GCCGGGCGGG TGGTGCTGCA GGTGGCCTGC GGGCTCTCCC ACAGCCTGGC CCTGGTCCAG 
TGCCCGCCCG CCCTCGACCG GAAGCCGCTC CCCGAGAGAT GCCGCCACTG CAGCCAGCTC 
CTGATCACCA TGACGGACAA AGAAGACCAC GTCATCATAT CGGACAGTCA CTGCTGCCCT 
CTGGGAGTGG TCCTCCCCGA CGTCCAGGAG GACGGCGGCA CCGGGCCCAA GCCCCCGCTG 
GGGCCCCCGG ACGGGGGAGG CCCGGCCGAG GCCCCGGGCC AGGGCCGGGG AAGGGGCCCA 
GTGGGGGCCC ACGCGCCTGT GGCTCCCGAA GGGCCCGTCG GCCCGGCCCA GGGTGTGGGA 
GGGGAGGCCG AACCCTGCCC GCCGGAGGCG AGCGGGGCCC AGGGGCCGGA CGGGGCCGGG 
GGTGGGCCCA CAGACACCGA CGAGGGGCTG CGGAAGGCGT CGGATCCCTC AGGAAGAGAG 
GATGCCGGGA GAAGAGCCAA CGTGGAGCCG GCTCAGTTTC AACCTCTTAT GGAAGAAGCG 
AGCCCCTGCC TCCCCAGCCC CCCGACGCTG GGCTCGTCCG CCCTAAACAG CTTGGTGGTC 
TCCTGCGCCT CGGCCGTGGG GGTCCACATC GCCGCCACCT ACGAAGCCGG CGCGCTGTCG 
CTCAGGAAGG TGATGAACTT TTCCGGCGAG GCGGGCTCGC CGTCGGGCCC CGAGGGGCCG 
AGAGACTGCC GGGAGGAGCA GGTCAAACTG GAATCGCTGC AGGGCAAGAA GAGCTCCAGC 
CTCGTGGACA TCAGAGAGGA GGAAGCCGAG GGCAGCAGTC GGAGGCTTTC CCTGCCCGGG 
TTGCTCTCCC AAGTCTCCCC CAGGCTCTTG AGGAAGGCGG CGCGGGCCAA GCCGCGGACG 
GTGGTGCTGA CCCCGACGTA CAGCGGCGAA GCGGACGCGC TGCTCCCGTC GCTGAGGACG 
GAGGTGTGGA GCTGGGGGAA GGGGAAGGAA GGCCAGCTGG GGCACGGGGA TGTCCTGCCC 
AGGCTTCAGC CCCTGTGTGT AAAATGCCTC GATGGTAAAG AAGTCATCCA CCTGGCCGCC 
GGGGACCACC ATTCCCTGGC CCTCACAGCC AAATCCCAGG TCTTCTCCTG GGGAAGCAAT 
ACCTTTGGGC AGCTGGGACA CTCGGATTCT CCAACCACAG TTCCTCGTCT TGCAAAGGTG 
TGCGGTGAAA ACCGCATCTG GAGTTTCGCC GCCGGCCAGG ATTTCTCCCT CTTCCTAGTG 
GACACGGAAG ACTTCCAGCC TGGATTGTAT TACAGCGGCC GGCAGGACTC TCCAGAAGGT 
GACGGTGGCC CAGAGAACGC CGTCGCCACA AAGTCTCCCA GGCTGCTGAC GTCTTGTAGC 
AAGCTGGGCT ACATCAGCAG TGTGACAGCC GGCAGGGACA GCTGTCTCGC CCTGGTGGAC 
AAAAACATCA TGGGCTACAT CGCCAGCCTG CATGAGCTGG CTGCTACGGA AAGACGATTC 
TATTCCATGC TGAGCACCGT CAAATCCCAG GTTCTCCGAC CTCTTCTCAG TTTAGATAAC 
TTGGGCGCCC CAGCTTCGGT CCAGCTCCTT CAAGAGGTGG CCAGCAGGTT CAGCAAGCTG 
TGTTACCTGA TTGGGCAGCA CGCGGCCTCC TTGAGCATCT TCCTCCGCGG GATAAAAGAA 
GTGCGGAGCC TGACGGTCCT GAAGCATGCA AGTCTCTTCT TGGATAGTTA CACAGAGTAC 
TGCACGGCCC TGACCAATTT TATCGTCATG GGAGGATTCC AGCTCCTCGC CAAACCGGCC 
ATGGACTTCT TAAATAAAAA CCAGGAGCTG TTACAGGAGC TGTCGGAGAC GAAAGACGGG 
GACGTCCAGC TGTTGGAGAT GCTGAATGCC TTATTCTTCC TCCCAATCCG GCGGCTTCAT 
GATTACGCCC GAGCTCTGCT AAAGCTGGCT ACATGTTTTG AGGCCTCTCC GGAGTACCAG 
ACGCTGCACG ATTCCAGTTC CTGCTACGAG ACCCTCGCTC TCCACCTTGG CAGGAAAAGG 
AAGGAAGCAG AAGCCACGCT GAGCTTCTGG AAGACGTTCC CCGGAAAGAT GACGGACTCC 
TTGAGGAAGC CAGAGCGCAG GCTAATCTGC GAGAGCAGCA ATCGAGCGCT GGCCCTGCAG 
CATGCTGGGA GATTCTCCGT GAATTGGTTC ATTCTCTTTA ACGATGCCTT GGTCCATGCC 
CAGTTCTCGA CCCATCACAT CTTCCCCCTG TCCACTCTGT GGGTCGAGCC TATCTCAGAA 
GAAGGCAACA GCGCGAACGG CCTGAAGTTG ACGACGCCGG AGGAACAGTT CACCCTCATT 
TCCTCGACCC CGCAGGAGAA GACCAAGTGG CTGCGGGCCC TGAGCCAGGC GGTGGACCAG 
GCCCTGAGGG GCGCGGCCGA CTTCCCCCCC TACGGCAGCG GCGCCCCGCG GCCGGACCCC 
CCCGCCTCCC GCAGCGCCAG CTACACCTTC TACAAGGCCT CCCGCCTCAA GGAAGCCACC 
TACGAGGGCC GCTGGCTCTC CGGGAAGCCC CACGGCAGAG GGGTCTTGAA GTGGACCGAC 
GGCAAGACGT ACTCCGGTAT GTTTCGGAAT GGCCTGGAAG ATGGATACGG GGAATATAAA 
GTCCCAAACA AAGCACTTAA CAAAGAAGAC CACTACGTCG GGTACTGGAA AGAAGGAAAA 
ATGTGCGGAC AAGGAGTTTA CAGCTACGCC ACGGGCGAAG TGTACGAAGG CTACTTCCAA 
GAGAACGTCC GCCACGGCCA CGGGCTTCTC CGCAGCGGCA AGCTGACCTC CACCTCCCCC 
AGCATGTTCA TCGGCCAGTG GACCATGGAC AAGAAGGCCG GATACGGCGT CTTCGACGAC 
ATCACCAGGG GAGAAAAGTA CATGGGCATG TGGCAAGATG ACATGTGTGA GGGCAACGGC 
GTGGTGGTCA CTCAGTTCGG ACTCTATTAC GAAGGCGCCT TTAGCCTTAA CAAAATGATG 
GGAACTGGAA TTTTGCTCTC TGAAGATGAT ACAGTCTACG AGGGAGAATT TTCAGATGAC 
TGGACCCTCA ATGGAAAGGG GACGCTGACC CTGCTGAATG GAGATTACAT CGAAGGCCAT 
TTTAGCGGAG AATGGGGGTC TGGGATCAAA ATCACCGGAA CCTACTTCAA ACCCAGCCTG 
TACGAGAGCG ATAAGGAGCG GCCCAAGGCC GTGAAGCTCG GAAACCTGGC CGTGGCGGCG 
GACGAGAAGT GGACGGCGGT GTTCGAGGAG TGCTGGCGAC AGCTGGGCTG CGAGTCTCCC 
GGGCAGGGGG AGAGCTGGAA GGCCTGGGAT AACATCGCCG TGGCCCTGAC CGCCAACAGA 
CGCCAGCACC GAGACAGCCC CGAGATCCTA AGTCGTTCGC ACACTCAGAC GCTGGAGAGC 
CTGGAATTCA TCCCGCAGCA CGGCGGGGAC TTCACGGTGG AGAAATACGA AGACATCAAG 
AGGTATCTGA TGAAGGCCTG CGACACCCCT CTGCACCCTC TGGGGCAGCT GGTAGAGACC 
CTGGTGGCAG TGTACAGGAT GACTTACGTG GGCGTGGGAG CCAACCGGCG GTTACTACAG 
GAGGCCGTCA GAGAGATCCA GTCCTACCTG AAGCGCATCT TCCAGCTTGT CAGGTTCCTT 
TTTCCCGACC TTCCCGAAGA AGGCAGCACG ATTCCACTCT CCTCCAGTGT CCCACCGGAG 
AGGAAATCCT TCTGCACCGG AAAAGCCGAT TCCAGATCTG AATCTCCAGA GCCGGGCTAC 
GTGGTGACGA GCTCCGGGCT GCTGCTTCCC GTGCTGCTGC CTCGGCTCTA CCCGCCGCTG 
TTCACGCTGT ACGCCCTGGA CAACGAGCGG GAGGAAGACG CCTACTGGGA GTGCGTCCTG 
CGCCTCAACA AGCAGCCCGA CACGGCGCTC CTCAGCTTCC TGGGAGTGCA GCGGAAGTTT 
TGGCCTGACA ACGTGTCGCT CCTCGGAGAC AGCAGAAAGG TGCTGCCGAC CACGAAGGAG 
GCCTGTTTCG CCTCCGCGGT GGAGTGTCTG CAGCAGATCA GCACGACCTT CACTCCGTCG 
GACAAGTTGA AGGTGATCCA GCAGACCTTC GAGGAGATCT CCCAGAGCGT CCTGGCGTGG 
CTCCGAGAAG ACTTCCTGTG GTCCATGGAC GACCTGTTTC CCGTGTTCCT CTACGTGGTT 
CTCCGGGCCA GAATCCGCAA TTTGGGCTCG GAAGTCCACC TGATCGAGGA CCTGATGGAC 
CCCTACCTGC AGCACGGCGA GCAGGGCATC ATGTTCACCA CCCTGAAGGC CTGTTACTAC 
CAGATCCAGC GAGAGAAACT GAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ALS2 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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