LOC100077504 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100077504
Entrez Gene ID	100077504
Full Name	probable ATP-dependent RNA helicase DDX60
Synonyms	DDX60
Gene Type	protein-coding
Organism	Ornithorhynchus anatinus(platypus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029076583.1	XP_028932416.1	probable ATP-dependent RNA helicase DDX60 isoform X1
XM_007673079.2	XP_007671269.1	probable ATP-dependent RNA helicase DDX60
XM_029076584.1	XP_028932417.1	probable ATP-dependent RNA helicase DDX60 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC100077504 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100077504 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOc02531	XM_007673079.2 Latest version!	Ornithorhynchus anatinus probable ATP-dependent RNA helicase DDX60 (LOC100077504), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOc114081	XM_029076583.1 Latest version!	Ornithorhynchus anatinus probable ATP-dependent RNA helicase DDX60 (LOC100077504), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOc114082	XM_029076584.1 Latest version!	Ornithorhynchus anatinus probable ATP-dependent RNA helicase DDX60 (LOC100077504), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100077504 ( XM_007673079.2 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007671269.1 Ornithorhynchus anatinus probable ATP-dependent RNA helicase DDX60 (LOC100077504), mRNA.

Price & Availability↑

CloneID	OOc02531
Clone ID Related Accession (Same CDS sequence)	XM_007673079.2
Accession Version	XM_007673079.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1460563200000
Organism	Ornithorhynchus anatinus(platypus)
Product	probable ATP-dependent RNA helicase DDX60
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 15, 2016 this sequence version replaced XM_007673079.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTACCAGA GTCTCCACAC TCGTTTCTTA CGTACTGCAT TAATACAACA TCTAAAACAC 
AACACTGAGG TTACCATAAG TACAGATTTT TCCAATTGTT TATCACCTGA ATGGGAGATT 
TTCCTTAAAG AAAGTTTTCC ATATTTCATT ATAGTCTCTG ATGTAGGAAT GACAAAACAA 
CAAACGGACT ATTTAAATAT ATTCATCAGC CATACCCTTG GAAAGAAAAT CAATGTGGTA 
CTGACTTCAG GACAGGAATC CGATATCCTT CGAGCTTATG GGTACCACAT CCAGAGCTCA 
CCGAAGCACA GGAAATTCTT CAGTGAGCAT CAGGAAGAGC TGCAAGATGC TTCTGAGTCC 
ATAATTCAGC ATTTTTCCAA AACCCAGGAT TCGGAAATAC ATTTAAAGCA GCACTTGGAT 
GCCATTCAGA AAGAGGTGCA CCAAACACTA CCCCCACTTA ATAAACTGTG GCCAGAAGGA 
GCTGACATTC GACGAATTGT GTGCATTCTC TCATGCTCAG TGGGCTTGAA AACATATGGT 
GACATGTTAA AGGATGCAGA TGTCTTTGAA AAACTAAATG AAAGCACTGA AAACGATAAT 
CCTAAGGAAA GCAGAGAAGC ACTGTCCCTG CAGGCTGTGG CCGACCTTTA TAGGATGCAT 
TGTCTCTGTG TGGCTCTTCT TCTGCACCTG CCTCTTCCTC AGCGAGCTCG GAGCAGGATC 
ACAGGTTATG TTTGGAAGAA AAGCCTGTCC CCATTTTTGA GAATGAAATA CAGATGTGAA 
TTTGTCATTC TAAAGCAGCT AAATGCTATG AGCGACTGGA AAGTGGATTT CACCTACCTG 
CCTGACTTAA GTGATAATTT GTTGTGGCAA AACATTGCCC ACTATTATGA AGTAGACAGT 
TGCCAAGGAT TAAATCTAGA GTTAGGGGAT AAAATTTTAA AAGAATATCA AAACTTATGG 
AGCGTAGTAT TAAAACTGTC AGGAAATTGT GACTTTGGAG GACCAATTCC TGTGAGAAAC 
ACTTCAGAGG CTTTCCTTAG AGAGAAACCA GAAGCTGGAG AAACAAAAGA AGAGATTCCC 
AAGTTAGGGC TCATCCCAAT GGAATCATCC ATAGTGAACG ATTATGCAGG AGATGTTCTG 
AAAAGTCTGC CTTTTCTAAG CAGTGATGAC CCGGCTATCA ATTCACTTAT TAAGGAGAAG 
GAATTTGATG AGCTGACACA ATGGAATTTC AGCCGATCTC TCAGTGATGA TTATGAGAGA 
ACAAAACGCA GCCAGGATGT AAATACTAAA GACCCTAAAG AACGAAGAGG AATCCAGAAG 
TTACAGTCCT TTTATCAATT GCTTGGCCAG TCAATGCCAG GTAGTAGATC GACAAGAATT 
GTAAGCCAGG TGGCTGTAGC CACCAAAGCA AACCCTGCTG ACAAAAAGAA AGCAAAATTA 
CCTAGGAAAA AAGCAGACAT AATTGCCGAA GAAAATCTGA AAGTACAGAA AGCCAAAGTG 
GAAAATAAAG AGCAGGAGCA GTGGAGTACC CTGTGTGGAT CTATTGAAAA GGAAATTAAG 
ACAAATTTTG CTGGTGGATT AAAACGGTTG GAGACTTTTC TCAAGACATG CCAAAGCCAG 
TCGGTAAAAT TTCTCGCTGA GGTGGTAGGA TTGGATGCCT GCTTCAAAGC ATGGGTGGAG 
CACTGCCGAA AATCAGAAGA AAAATCAAAA GATTTGGACA TAGCAGTGCA GGTGATGCAA 
AGGATCCATA CAATCTATGG AAAATATCAA GAACACTTAC AGAAGACACA TCTACAAAAA 
CTGGCTAAAT ATCTAAAATA CCTTGGATTT GACAACCTGG CATGCTCATT ATCTTCTCAG 
ATAATGGAGA AAGGCAGCGA CAAGGAGATT TCAAAGTACT CTGTTCAGAT GGGAGCTGCT 
CGATTTCAGC TGCAACAGAT GGGGCATCAT TTGTTCAGGG ATGAGAGATC GGATCCGGAC 
CCCAGAGTAC AGTATTTTAT ACCAGACACG TGGCAGCGAG AACTTCTCGA TGTCGTGGAC 
AATAATGAGT CTGCTTTGAT CGTTGCTCCA ACTTCATCTG GTAAAACATA CGCCTCATAC 
TACTGCATGG AGAAAGTGCT GAAAGAGAGC GATGAAGGGG TCGTTGTCTA TGTTTCTCCA 
TCAAAGGCTC TTATAAATCA AGTGGTCGCA ACTATATATA ACCAGTTTAC CAAAAGCCTG 
CCTCACGGAT TAGTAATATA TGGAGTTTTC ACGAGGGATT ACCGCACAGA TGCCATGAAC 
TGCCAGATTC TCGTGACGGT ACCTGAGTGT TTAGAAATCC TGCTGCTAGC CCCTCAACAC 
CAGCAATGGG CTCAGAAGAT CAGATATGTT ATATTTGATG AGGTCCATTG TCTAGGCGGA 
GAAATCGGAG CAGACGTTTG GGAACACATC CTTGTTATGA TCCGCTGCCC CTTTTTGGCT 
CTGTCTGCAA CCATAAGTAA CCCAAAGCAC CTCACTGAGT GGTTGCAGTT GGTGAAAAGG 
TACTGGCAGA ATGTGGAAAA CACAACGGAA AGCTCTGTAT CTGCAAATGC CCTAAAAGGT 
TCAAAGAAAC AAAAGGTTCA AAAGGCAGAG AAGAAATCAC ATAGAGTTCG ACTTGTACAG 
TATGAAGAGA GGTACAATGA TCTAGAAAAA TACGTGTGCT CTCTGAAGAA TGATGACTTT 
GCCATCGATC ACTATCATCC ATTTGCCGCA TTAACTGTCA ATCATATTGA AAAATATGGA 
ATTCCCTCAG ACCTTGCATT TTCCCCACGA GAAAGCATTC AACTCTATGA CGTCATGGCA 
GATATTTGGC AGGAATGGCC CAGAGCACAG GAACTGCATC CTGAAGAATA CAGTGCTTTC 
AAGAATAAAG TAGTGATCAC TAAAGCAGAT GCAAAAAATT ATGAAAGGGA ACTGAAGAAA 
GAAATAACCA GCTGGATTGA GCTTGGCCAT AGAGAAAAGG TGAACCAACT ATTGGAGTAC 
CTTAAACCTC AAGTCGATGA TAATCCGGAA AATGAAAAAG TAAAGTTTGC ACGTTTTGTT 
GAGAAGCTAA AAGAGATTGA TAAATTGCCT GCAATTTTTT TTATGTTCCA AATCTCTTCA 
GTACGTGATT TTGCCAAGAT TGTAAGTCAG TATTTGGAAG AGAAACAGAA GAATAAACAA 
GATTCAAATG TTGAGAAACA AGAACAATAT TTAAAAGAAA AAATTAAGAA GTTGGAAAAG 
TCCATGGAAA AAACAAGATC AGCATTGGAG AAAGGAAGTC CCAGCAATAA ACTCGAGAGC 
ACCATTGTTA CTTTCGAGAA TGAAAGAAGT CAATTAGAGG AGAAACTTGC TAAACTTACT 
GAAATTCCTC CGGACTGTAC TTTCGCTAAC AGTAAAGCAG TAGATAATGA AACCTGGACC 
AAAATCTTTA GTCGACTCAA GTATTCGGCA AATGGCAAAG TACTGACTGA TCTGGCACTC 
CGTGGCATCG GCTACCATCA TGAATTCATG AGAATCCGAG AGAGACAAGT GGTGGAGATG 
CTCTTCAGGC TGGGATATAT CAAGGTTGGT ATGTCCGGGC GTGCAGGAAG ACGAGGCGAA 
GACCTGATAG GAAATGTCTT CTTCTACGAT ATTCCGTTGC CCAAAGTTGG GCAGCTGTTG 
AAGTCTAACG TCCCGCTGCT AAAAGGCCAG TTCCCTCTGA ACATTTCCCT GGTCCTGCGA 
CTCATGCTTC TGGCCGCAAA GGCCGATGAC AAGGAGGATG CCAAGGCCAA GGTGTTGTCT 
GTCCTGCAGC ATCCATTGAT GGCCTTCAGA AAAGAGAAGA TCACAGAACT GTTAAAAGTG 
TACTTTATAT TTTCCTTGCA GTTTCTTATT AAAGAGGGAT ATCTGGATCA AGAATGCAAC 
CCCAGGGGAT TTTCTGAACT GGTGGCCAGT TTGCACAAAC ACAAGCCTTC AAATTTTGTG 
TTCGTGAGCT TTCTCGAGAG GGGCTTATTT CACAAGTTGT GTAAGCCAAC CCAAGAGGGC 
TCAAAGGTTT TCTCTGAAGA TATAATGGAA ACACTAGTGC TCATCCTAGC AAATCTTTTT 
GGAAGACAAT ATTTGCCGTC CTCGACGGTT TGCCAATCAG AGGAGTTCCT AGGCGATCTC 
CCGGAAGACT TCGCCGCCGC AGTTGAGGAG TACAATAGCA AAATCCAGAA GAACTTTGGC 
TATTTCCTGC TTACAGTCTC CAAACTGGCT GACATGAAAC AAGAATGCAA GCTGCCTCTG 
TCGGGAATTG ATTTTGCAGG TACAGAATTC AAGGACTCGG AACTGGCATC TCATTTACTG 
AGCGGCAAGG AGAGCAGAAC CGCTGTCTCG CCTTTCGCTT GCCTCTCTGA ACTGGTGGAC 
CAGGATCTCT TTGATACCAG TGTGGTAAAC GATGCGATCC TGCGAACGGT GGGTGTAAGT 
TCTTCCAGCG CTCCTGTGTT CTATTTGAAG AAATTTGATG AACACGGGAG AAGATGCCCA 
CTCAGTGCTT ATGCCCTTGA CTTCTATAGA CAGGGCTCCC TGGAATTCCT GACTCAGGAG 
ATGTGGGATC TGCTCAAAAA CTTTACACAT CTTATCAATT CCATCAGGAC ATCTTTAGAA 
GAATTGTGTG AGAACGAGGA TGACAATGTT CTGCTGGCAT TTAAACAACT GAGCGACAGC 
TACAGTGAAA AGTTTAGCAA AGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007671269.1
CDS	31..4737
Translation	MYQSLHTRFLRTALIQHLKHNTEVTISTDFSNCLSPEWEIFLKESFPYFIIVSDVGMTKQQTDYLNIFISHTLGKKINVVLTSGQESDILRAYGYHIQSSPKHRKFFSEHQEELQDASESIIQHFSKTQDSEIHLKQHLDAIQKEVHQTLPPLNKLWPEGADIRRIVCILSCSVGLKTYGDMLKDADVFEKLNESTENDNPKESREALSLQAVADLYRMHCLCVALLLHLPLPQRARSRITGYVWKKSLSPFLRMKYRCEFVILKQLNAMSDWKVDFTYLPDLSDNLLWQNIAHYYEVDSCQGLNLELGDKILKEYQNLWSVVLKLSGNCDFGGPIPVRNTSEAFLREKPEAGETKEEIPKLGLIPMESSIVNDYAGDVLKSLPFLSSDDPAINSLIKEKEFDELTQWNFSRSLSDDYERTKRSQDVNTKDPKERRGIQKLQSFYQLLGQSMPGSRSTRIVSQVAVATKANPADKKKAKLPRKKADIIAEENLKVQKAKVENKEQEQWSTLCGSIEKEIKTNFAGGLKRLETFLKTCQSQSVKFLAEVVGLDACFKAWVEHCRKSEEKSKDLDIAVQVMQRIHTIYGKYQEHLQKTHLQKLAKYLKYLGFDNLACSLSSQIMEKGSDKEISKYSVQMGAARFQLQQMGHHLFRDERSDPDPRVQYFIPDTWQRELLDVVDNNESALIVAPTSSGKTYASYYCMEKVLKESDEGVVVYVSPSKALINQVVATIYNQFTKSLPHGLVIYGVFTRDYRTDAMNCQILVTVPECLEILLLAPQHQQWAQKIRYVIFDEVHCLGGEIGADVWEHILVMIRCPFLALSATISNPKHLTEWLQLVKRYWQNVENTTESSVSANALKGSKKQKVQKAEKKSHRVRLVQYEERYNDLEKYVCSLKNDDFAIDHYHPFAALTVNHIEKYGIPSDLAFSPRESIQLYDVMADIWQEWPRAQELHPEEYSAFKNKVVITKADAKNYERELKKEITSWIELGHREKVNQLLEYLKPQVDDNPENEKVKFARFVEKLKEIDKLPAIFFMFQISSVRDFAKIVSQYLEEKQKNKQDSNVEKQEQYLKEKIKKLEKSMEKTRSALEKGSPSNKLESTIVTFENERSQLEEKLAKLTEIPPDCTFANSKAVDNETWTKIFSRLKYSANGKVLTDLALRGIGYHHEFMRIRERQVVEMLFRLGYIKVGMSGRAGRRGEDLIGNVFFYDIPLPKVGQLLKSNVPLLKGQFPLNISLVLRLMLLAAKADDKEDAKAKVLSVLQHPLMAFRKEKITELLKVYFIFSLQFLIKEGYLDQECNPRGFSELVASLHKHKPSNFVFVSFLERGLFHKLCKPTQEGSKVFSEDIMETLVLILANLFGRQYLPSSTVCQSEEFLGDLPEDFAAAVEEYNSKIQKNFGYFLLTVSKLADMKQECKLPLSGIDFAGTEFKDSELASHLLSGKESRTAVSPFACLSELVDQDLFDTSVVNDAILRTVGVSSSSAPVFYLKKFDEHGRRCPLSAYALDFYRQGSLEFLTQEMWDLLKNFTHLINSIRTSLEELCENEDDNVLLAFKQLSDSYSEKFSKV

Target ORF information:

RefSeq Version	XM_007673079.2
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus probable ATP-dependent RNA helicase DDX60 (LOC100077504), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007673079.2

ORF Insert Sequence:

ATGTACCAGA GTCTCCACAC TCGTTTCTTA CGTACTGCAT TAATACAACA TCTAAAACAC 
AACACTGAGG TTACCATAAG TACAGATTTT TCCAATTGTT TATCACCTGA ATGGGAGATT 
TTCCTTAAAG AAAGTTTTCC ATATTTCATT ATAGTCTCTG ATGTAGGAAT GACAAAACAA 
CAAACGGACT ATTTAAATAT ATTCATCAGC CATACCCTTG GAAAGAAAAT CAATGTGGTA 
CTGACTTCAG GACAGGAATC CGATATCCTT CGAGCTTATG GGTACCACAT CCAGAGCTCA 
CCGAAGCACA GGAAATTCTT CAGTGAGCAT CAGGAAGAGC TGCAAGATGC TTCTGAGTCC 
ATAATTCAGC ATTTTTCCAA AACCCAGGAT TCGGAAATAC ATTTAAAGCA GCACTTGGAT 
GCCATTCAGA AAGAGGTGCA CCAAACACTA CCCCCACTTA ATAAACTGTG GCCAGAAGGA 
GCTGACATTC GACGAATTGT GTGCATTCTC TCATGCTCAG TGGGCTTGAA AACATATGGT 
GACATGTTAA AGGATGCAGA TGTCTTTGAA AAACTAAATG AAAGCACTGA AAACGATAAT 
CCTAAGGAAA GCAGAGAAGC ACTGTCCCTG CAGGCTGTGG CCGACCTTTA TAGGATGCAT 
TGTCTCTGTG TGGCTCTTCT TCTGCACCTG CCTCTTCCTC AGCGAGCTCG GAGCAGGATC 
ACAGGTTATG TTTGGAAGAA AAGCCTGTCC CCATTTTTGA GAATGAAATA CAGATGTGAA 
TTTGTCATTC TAAAGCAGCT AAATGCTATG AGCGACTGGA AAGTGGATTT CACCTACCTG 
CCTGACTTAA GTGATAATTT GTTGTGGCAA AACATTGCCC ACTATTATGA AGTAGACAGT 
TGCCAAGGAT TAAATCTAGA GTTAGGGGAT AAAATTTTAA AAGAATATCA AAACTTATGG 
AGCGTAGTAT TAAAACTGTC AGGAAATTGT GACTTTGGAG GACCAATTCC TGTGAGAAAC 
ACTTCAGAGG CTTTCCTTAG AGAGAAACCA GAAGCTGGAG AAACAAAAGA AGAGATTCCC 
AAGTTAGGGC TCATCCCAAT GGAATCATCC ATAGTGAACG ATTATGCAGG AGATGTTCTG 
AAAAGTCTGC CTTTTCTAAG CAGTGATGAC CCGGCTATCA ATTCACTTAT TAAGGAGAAG 
GAATTTGATG AGCTGACACA ATGGAATTTC AGCCGATCTC TCAGTGATGA TTATGAGAGA 
ACAAAACGCA GCCAGGATGT AAATACTAAA GACCCTAAAG AACGAAGAGG AATCCAGAAG 
TTACAGTCCT TTTATCAATT GCTTGGCCAG TCAATGCCAG GTAGTAGATC GACAAGAATT 
GTAAGCCAGG TGGCTGTAGC CACCAAAGCA AACCCTGCTG ACAAAAAGAA AGCAAAATTA 
CCTAGGAAAA AAGCAGACAT AATTGCCGAA GAAAATCTGA AAGTACAGAA AGCCAAAGTG 
GAAAATAAAG AGCAGGAGCA GTGGAGTACC CTGTGTGGAT CTATTGAAAA GGAAATTAAG 
ACAAATTTTG CTGGTGGATT AAAACGGTTG GAGACTTTTC TCAAGACATG CCAAAGCCAG 
TCGGTAAAAT TTCTCGCTGA GGTGGTAGGA TTGGATGCCT GCTTCAAAGC ATGGGTGGAG 
CACTGCCGAA AATCAGAAGA AAAATCAAAA GATTTGGACA TAGCAGTGCA GGTGATGCAA 
AGGATCCATA CAATCTATGG AAAATATCAA GAACACTTAC AGAAGACACA TCTACAAAAA 
CTGGCTAAAT ATCTAAAATA CCTTGGATTT GACAACCTGG CATGCTCATT ATCTTCTCAG 
ATAATGGAGA AAGGCAGCGA CAAGGAGATT TCAAAGTACT CTGTTCAGAT GGGAGCTGCT 
CGATTTCAGC TGCAACAGAT GGGGCATCAT TTGTTCAGGG ATGAGAGATC GGATCCGGAC 
CCCAGAGTAC AGTATTTTAT ACCAGACACG TGGCAGCGAG AACTTCTCGA TGTCGTGGAC 
AATAATGAGT CTGCTTTGAT CGTTGCTCCA ACTTCATCTG GTAAAACATA CGCCTCATAC 
TACTGCATGG AGAAAGTGCT GAAAGAGAGC GATGAAGGGG TCGTTGTCTA TGTTTCTCCA 
TCAAAGGCTC TTATAAATCA AGTGGTCGCA ACTATATATA ACCAGTTTAC CAAAAGCCTG 
CCTCACGGAT TAGTAATATA TGGAGTTTTC ACGAGGGATT ACCGCACAGA TGCCATGAAC 
TGCCAGATTC TCGTGACGGT ACCTGAGTGT TTAGAAATCC TGCTGCTAGC CCCTCAACAC 
CAGCAATGGG CTCAGAAGAT CAGATATGTT ATATTTGATG AGGTCCATTG TCTAGGCGGA 
GAAATCGGAG CAGACGTTTG GGAACACATC CTTGTTATGA TCCGCTGCCC CTTTTTGGCT 
CTGTCTGCAA CCATAAGTAA CCCAAAGCAC CTCACTGAGT GGTTGCAGTT GGTGAAAAGG 
TACTGGCAGA ATGTGGAAAA CACAACGGAA AGCTCTGTAT CTGCAAATGC CCTAAAAGGT 
TCAAAGAAAC AAAAGGTTCA AAAGGCAGAG AAGAAATCAC ATAGAGTTCG ACTTGTACAG 
TATGAAGAGA GGTACAATGA TCTAGAAAAA TACGTGTGCT CTCTGAAGAA TGATGACTTT 
GCCATCGATC ACTATCATCC ATTTGCCGCA TTAACTGTCA ATCATATTGA AAAATATGGA 
ATTCCCTCAG ACCTTGCATT TTCCCCACGA GAAAGCATTC AACTCTATGA CGTCATGGCA 
GATATTTGGC AGGAATGGCC CAGAGCACAG GAACTGCATC CTGAAGAATA CAGTGCTTTC 
AAGAATAAAG TAGTGATCAC TAAAGCAGAT GCAAAAAATT ATGAAAGGGA ACTGAAGAAA 
GAAATAACCA GCTGGATTGA GCTTGGCCAT AGAGAAAAGG TGAACCAACT ATTGGAGTAC 
CTTAAACCTC AAGTCGATGA TAATCCGGAA AATGAAAAAG TAAAGTTTGC ACGTTTTGTT 
GAGAAGCTAA AAGAGATTGA TAAATTGCCT GCAATTTTTT TTATGTTCCA AATCTCTTCA 
GTACGTGATT TTGCCAAGAT TGTAAGTCAG TATTTGGAAG AGAAACAGAA GAATAAACAA 
GATTCAAATG TTGAGAAACA AGAACAATAT TTAAAAGAAA AAATTAAGAA GTTGGAAAAG 
TCCATGGAAA AAACAAGATC AGCATTGGAG AAAGGAAGTC CCAGCAATAA ACTCGAGAGC 
ACCATTGTTA CTTTCGAGAA TGAAAGAAGT CAATTAGAGG AGAAACTTGC TAAACTTACT 
GAAATTCCTC CGGACTGTAC TTTCGCTAAC AGTAAAGCAG TAGATAATGA AACCTGGACC 
AAAATCTTTA GTCGACTCAA GTATTCGGCA AATGGCAAAG TACTGACTGA TCTGGCACTC 
CGTGGCATCG GCTACCATCA TGAATTCATG AGAATCCGAG AGAGACAAGT GGTGGAGATG 
CTCTTCAGGC TGGGATATAT CAAGGTTGGT ATGTCCGGGC GTGCAGGAAG ACGAGGCGAA 
GACCTGATAG GAAATGTCTT CTTCTACGAT ATTCCGTTGC CCAAAGTTGG GCAGCTGTTG 
AAGTCTAACG TCCCGCTGCT AAAAGGCCAG TTCCCTCTGA ACATTTCCCT GGTCCTGCGA 
CTCATGCTTC TGGCCGCAAA GGCCGATGAC AAGGAGGATG CCAAGGCCAA GGTGTTGTCT 
GTCCTGCAGC ATCCATTGAT GGCCTTCAGA AAAGAGAAGA TCACAGAACT GTTAAAAGTG 
TACTTTATAT TTTCCTTGCA GTTTCTTATT AAAGAGGGAT ATCTGGATCA AGAATGCAAC 
CCCAGGGGAT TTTCTGAACT GGTGGCCAGT TTGCACAAAC ACAAGCCTTC AAATTTTGTG 
TTCGTGAGCT TTCTCGAGAG GGGCTTATTT CACAAGTTGT GTAAGCCAAC CCAAGAGGGC 
TCAAAGGTTT TCTCTGAAGA TATAATGGAA ACACTAGTGC TCATCCTAGC AAATCTTTTT 
GGAAGACAAT ATTTGCCGTC CTCGACGGTT TGCCAATCAG AGGAGTTCCT AGGCGATCTC 
CCGGAAGACT TCGCCGCCGC AGTTGAGGAG TACAATAGCA AAATCCAGAA GAACTTTGGC 
TATTTCCTGC TTACAGTCTC CAAACTGGCT GACATGAAAC AAGAATGCAA GCTGCCTCTG 
TCGGGAATTG ATTTTGCAGG TACAGAATTC AAGGACTCGG AACTGGCATC TCATTTACTG 
AGCGGCAAGG AGAGCAGAAC CGCTGTCTCG CCTTTCGCTT GCCTCTCTGA ACTGGTGGAC 
CAGGATCTCT TTGATACCAG TGTGGTAAAC GATGCGATCC TGCGAACGGT GGGTGTAAGT 
TCTTCCAGCG CTCCTGTGTT CTATTTGAAG AAATTTGATG AACACGGGAG AAGATGCCCA 
CTCAGTGCTT ATGCCCTTGA CTTCTATAGA CAGGGCTCCC TGGAATTCCT GACTCAGGAG 
ATGTGGGATC TGCTCAAAAA CTTTACACAT CTTATCAATT CCATCAGGAC ATCTTTAGAA 
GAATTGTGTG AGAACGAGGA TGACAATGTT CTGCTGGCAT TTAAACAACT GAGCGACAGC 
TACAGTGAAA AGTTTAGCAA AGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100077504 ( XM_029076583.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028932416.1 Ornithorhynchus anatinus probable ATP-dependent RNA helicase DDX60 (LOC100077504), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOc114081
Clone ID Related Accession (Same CDS sequence)	XM_029076583.1
Accession Version	XM_029076583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556380800000
Organism	Ornithorhynchus anatinus(platypus)
Product	probable ATP-dependent RNA helicase DDX60 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTG GAGAAGCTGC GTTAGAGGAA GACGTCAATA ACGTTCAGAG TGACGCTGAT 
GAGGACAGTG AGCCCGAAGA AATTTTCTCA TCATTTGGTG ATTCTAAGGC ATCCGGTACA 
CCAGAATATG GAGGAAAATC CCAACCCGAC TCTGAAGCTG ATAGAGGAGA TTGTGAAGGC 
ACCGAGGCCA ATTCTGAGAT GCCAAATCCA GATCAATCAA AAGACATTAC CTTTCTGAAA 
AAATTGTCAT GTAACATTGA GGAGACTCTG TCACAAGCAA AGTACATCAG CTTACTTAAA 
GATTTTGTTG ACTCTGAATT TTTCTTGATT GATGGGGATT CATTATTTCT TACCGGTCTT 
TTCAACAAGA CTTTTAAATC AGGGCAACAT CTTCATTTCT TCTACTTGAT AGAACGCTTT 
CTGGATGATT TCTCTCAGAA AGGAGTGAAA TATGTCATTG TATTTTTTAA GGATGTAGAA 
AATATGTACC AGAGTCTCCA CACTCGTTTC TTACGTACTG CATTAATACA ACATCTAAAA 
CACAACACTG AGGTTACCAT AAGTACAGAT TTTTCCAATT GTTTATCACC TGAATGGGAG 
ATTTTCCTTA AAGAAAGTTT TCCATATTTC ATTATAGTCT CTGATGTAGG AATGACAAAA 
CAACAAACGG ACTATTTAAA TATATTCATC AGCCATACCC TTGGAAAGAA AATCAATGTG 
GTACTGACTT CAGGACAGGA ATCCGATATC CTTCGAGCTT ATGGGTACCA CATCCAGAGC 
TCACCGAAGC ACAGGAAATT CTTCAGTGAG CATCAGGAAG AGCTGCAAGA TGCTTGTGAG 
TCCATAATTC AGCATTTTTC CAAAACCCAG GATTCGGAAA TACATTTAAA GCAGCACTTG 
GATGCCATTC AGAAAGAGGT GCACCAAACA CTACCCCCAC TTAATAAACT GTGGCCAGAA 
GGAGCTGACA TTCGACGAAT TGTGTGCATT CTCTCATGCT CAGTGGGCTT GAAAACATAT 
GGTGACATGT TAAAGGATGC AGATGTCTTT GAAAAACTAA ATGAAAGCAC TGAAAACGAT 
AATCCTAAGG AAAGCAGAGA AGCACTGTCC CTGCAGGCTG TGGCCGACCT TTATAGGATG 
CATTGTCTCT GTGTGGCTCT TCTTCTGCAC CTGCCTCTTC CTCAGCGAGC TCGGAGCAGG 
ATCACAGGTT ATGTTTGGAA GAAAAGCCTG TCCCCATTTT TGAGAATGAA ATACAGATGT 
GAATTTGTCA TTCTAAAGCA GCTAAATGCT ATGAGCGACT GGAAAGTGGA TTTCACCTAC 
CTGCCTGACT TAAGTGATAA TTTGTTGTGG CAAAACATTG CCCACTATTA TGAAGTAGAC 
AGTTGCCAAG GATTAAATCT AGAGTTAGGG GATAAAATTT TAAAAGAATA TCAAAACTTA 
TGGAGCGTAG TATTAAAACT GTCAGGAAAT TGTGACTTTG GAGGACCAAT TCCTGTGAGA 
AACACTTCAG AGGCTTTCCT TAGAGAGAAA CCAGAAGCTG GAGAAACAAA AGAAGAGATT 
CCCAAGTTAG GGCTCATCCC AATGGAATCA TCCATAGTGA ACGATTATGC AGGAGATGTT 
CTGAAAAGTC TGCCTTTTCT AAGCAGTGAT GACCCGGCTA TCAATTCACT TATTAAGGAG 
AAGGAATTTG ATGAGCTGAC ACAATGGAAT TTCAGCCGAT CTCTCAGTGA TGATTATGAG 
AGAACAAAAC GCAGCCAGGA TGTAAATACT AAAGACCCTA AAGAACGAAG AGGAATCCAG 
AAGTTACAGT CCTTTTATCA ATTGCTTGGC CAGTCAATGC CAGGTAGTAG ATCGACAAGA 
ATTGTAAGCC AGGTGGCTGT AGCCACCAAA GCAAACCCTG CTGACAAAAA GAAAGCAAAA 
TTACCTAGGA AAAAAGCAGA CATAATTGCC GAAGAAAATC TGAAAGTACA GAAAGCCAAA 
GTGGAAAATA AAGAGCAGGA GCAGTGGAGT ACCCTGTGTG GATCTATTGA AAAGGAAATT 
AAGACAAATT TTGCTGGTGG ATTAAAACGG TTGGAGACTT TTCTCAAGAC ATGCCAAAGC 
CAGTCGGTAA AATTTCTCGC TGAGGTGGTA GGATTGGATG CCTGCTTCAA AGCATGGGTG 
GAGCACTGCC GAAAATCAGA AGAAAAATCA AAAGATTTGG ACATAGCAGT GCAGGTGATG 
CAAAGGATCC ATACAATCTA TGGAAAATAT CAAGAACACT TACAGAAGAC ACATCTACAA 
AAACTGGCTA AATATCTAAA ATACCTTGGA TTTGACAACC TGGCATGCTC ATTATCTTCT 
CAGATAATGG AGAAAGGCAG CGACAAGGAG ATTTCAAAGT ACTCTGTTCA GATGGGAGCT 
GCTCGATTTC AGCTGCAACA GATGGGGCAT CATTTGTTCA GGGATGAGAG ATCGGATCCG 
GACCCCAGAG TACAGTATTT TATACCAGAC ACGTGGCAGC GAGAACTTCT CGATGTCGTG 
GACAATAATG AGTCTGCTTT GATCGTTGCT CCAACTTCAT CTGGTAAAAC ATACGCCTCA 
TACTACTGCA TGGAGAAAGT GCTGAAAGAG AGCGATGAAG GGGTCGTTGT CTATGTTTCT 
CCATCAAAGG CTCTTATAAA TCAAGTGGTC GCAACTATAT ATAACCAGTT TACCAAAAGC 
CTGCCTCACG GATTAGTAAT ATATGGAGTT TTCACGAGGG ATTACCGCAC AGATGCCATG 
AACTGCCAGA TTCTCGTGAC GGTACCTGAG TGTTTAGAAA TCCTGCTGCT AGCCCCTCAA 
CACCAGCAAT GGGCTCAGAA GATCAGATAT GTTATATTTG ATGAGGTCCA TTGTCTAGGC 
GGAGAAATCG GAGCAGACGT TTGGGAACAC ATCCTTGTTA TGATCCGCTG CCCCTTTTTG 
GCTCTGTCTG CAACCATAAG TAACCCAAAG CACCTCACTG AGTGGTTGCA GTTGGTGAAA 
AGGTACTGGC AGAATGTGGA AAACACAACG GAAAGCTCTG TATCTGCAAA TGCCCTAAAA 
GGTTCAAAGA AACAAAAGGT TCAAAAGGCA GAGAAGAAAT CACATAGAGT TCGACTTGTA 
CAGTATGAAG AGAGGTACAA TGATCTAGAA AAATACGTGT GCTCTCTGAA GAATGATGAC 
TTTGCCATCG ATCACTATCA TCCATTTGCC GCATTAACTG TCAATCATAT TGAAAAATAT 
GGAATTCCCT CAGACCTTGC ATTTTCCCCA CGAGAAAGCA TTCAACTCTA TGACGTCATG 
GCAGATATTT GGCAGGAATG GCCCAGAGCA CAGGAACTGC ATCCTGAAGA ATACAGTGCT 
TTCAAGAATA AAGTAGTGAT CACTAAAGCA GATGCAAAAA ATTATGAAAG GGAACTGAAG 
AAAGAAATAA CCAGCTGGAT TGAGCTTGGC CATAGAGAAA AGGTGAACCA ACTATTGGAG 
TACCTTAAAC CTCAAGTCGA TGATAATCCG GAAAATGAAA AAGTAAAGTT TGCACGTTTT 
GTTGAGAAGC TAAAAGAGAT TGATAAATTG CCTGCAATTT TTTTTATGTT CCAAATCTCT 
TCAGTACGTG ATTTTGCCAA GATTGTAAGT CAGTATTTGG AAGAGAAACA GAAGAATAAA 
CAAGATTCAA ATGTTGAGAA ACAAGAACAA TATTTAAAAG AAAAAATTAA GAAGTTGGAA 
AAGTCCATGG AAAAAACAAG ATCAGCATTG GAGAAAGGAA GTCCCAGCAA TAAACTCGAG 
AGCACCATTG TTACTTTCGA GAATGAAAGA AGTCAATTAG AGGAGAAACT TGCTAAACTT 
ACTGAAATTC CTCCGGACTG TACTTTCGCT AACAGTAAAG CAGTAGATAA TGAAACCTGG 
ACCAAAATCT TTAGTCGACT CAAGTATTCG GCAAATGGCA AAGTACTGAC TGATCTGGCA 
CTCCGTGGCA TCGGCTACCA TCATGAATTC ATGAGAATCC GAGAGAGACA AGTGGTGGAG 
ATGCTCTTCA GGCTGGGATA TATCAAGGTT GTGACGGCTA CTGGAACCCT GGCTTTGGGT 
ATTAACATGC CGTGCAAATC TGTTGTCTTC CCTCAAGATT CCGTCTTTCT GGATTCCCTC 
AATTACAGAC AGATGTCCGG GCGTGCAGGA AGACGAGGCG AAGACCTGAT AGGAAATGTC 
TTCTTCTACG ATATTCCGTT GCCCAAAGTT GGGCAGCTGT TGAAGTCTAA CGTCCCGCTG 
CTAAAAGGCC AGTTCCCTCT GAACATTTCC CTGGTCCTGC GACTCATGCT TCTGGCCGCA 
AAGGCCGATG ACAAGGAGGA TGCCAAGGCC AAGGTGTTGT CTGTCCTGCA GCATCCATTG 
ATGGCCTTCA GAAAAGAGAA GATCACAGAA CTGTTAAAAG TGTACTTTAT ATTTTCCTTG 
CAGTTTCTTA TTAAAGAGGG ATATCTGGAT CAAGAATGCA ACCCCAGGGG ATTTTCTGAA 
CTGGTGGCCA GTTTGCACAA ACACAAGCCT TCAAATTTTG TGTTCGTGAG CTTTCTCGAG 
AGGGGCTTAT TTCACAAGTT GTGTAAGCCA ACCCAAGAGG GCTCAAAGGT TTTCTCTGAA 
GATATAATGG AAACACTAGT GCTCATCCTA GCAAATCTTT TTGGAAGACA ATATTTGCCG 
TCCTCGACGG TTTGCCAATC AGAGGAGTTC CTAGGCGATC TCCCGGAAGA CTTCGCCGCC 
GCAGTTGAGG AGTACAATAG CAAAATCCAG AAGAACTTTG GCTATTTCCT GCTTACAGTC 
TCCAAACTGG CTGACATGAA ACAAGAATGC AAGCTGCCTC TGTCGGGAAT TGATTTTGCA 
GGTACAGAAT TCAAGGACTC GGAACTGGCA TCTCATTTAC TGAGCGGCAA GGAGAGCAGA 
ACCGCTGTCT CGCCTTTCGC TTGCCTCTCT GAACTGGTGG ACCAGGATCT CTTTGATACC 
AGTGTGGTAA ACGATGCGAT CCTGCGAACG GTGGGTGTAA GTTCTTCCAG CGCTCCTGTG 
TTCTATTTGA AGAAATTTGA TGAACACGGG AGAAGATGCC CACTCAGTGC TTATGCCCTT 
GACTTCTATA GACAGGGCTC CCTGGAATTC CTGACTCAGG AGATGTGGGA TCTGCTCAAA 
AACTTTACAC ATCTTATCAA TTCCATCAGG ACATCTTTAG AAGAATTGTG TGAGAACGAG 
GATGACAATG TTCTGCTGGC ATTTAAACAA CTGAGCGACA GCTACAGTGA AAAGTTTAGC 
AAAGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028932416.1
CDS	114..5402
Translation	MASGEAALEEDVNNVQSDADEDSEPEEIFSSFGDSKASGTPEYGGKSQPDSEADRGDCEGTEANSEMPNPDQSKDITFLKKLSCNIEETLSQAKYISLLKDFVDSEFFLIDGDSLFLTGLFNKTFKSGQHLHFFYLIERFLDDFSQKGVKYVIVFFKDVENMYQSLHTRFLRTALIQHLKHNTEVTISTDFSNCLSPEWEIFLKESFPYFIIVSDVGMTKQQTDYLNIFISHTLGKKINVVLTSGQESDILRAYGYHIQSSPKHRKFFSEHQEELQDACESIIQHFSKTQDSEIHLKQHLDAIQKEVHQTLPPLNKLWPEGADIRRIVCILSCSVGLKTYGDMLKDADVFEKLNESTENDNPKESREALSLQAVADLYRMHCLCVALLLHLPLPQRARSRITGYVWKKSLSPFLRMKYRCEFVILKQLNAMSDWKVDFTYLPDLSDNLLWQNIAHYYEVDSCQGLNLELGDKILKEYQNLWSVVLKLSGNCDFGGPIPVRNTSEAFLREKPEAGETKEEIPKLGLIPMESSIVNDYAGDVLKSLPFLSSDDPAINSLIKEKEFDELTQWNFSRSLSDDYERTKRSQDVNTKDPKERRGIQKLQSFYQLLGQSMPGSRSTRIVSQVAVATKANPADKKKAKLPRKKADIIAEENLKVQKAKVENKEQEQWSTLCGSIEKEIKTNFAGGLKRLETFLKTCQSQSVKFLAEVVGLDACFKAWVEHCRKSEEKSKDLDIAVQVMQRIHTIYGKYQEHLQKTHLQKLAKYLKYLGFDNLACSLSSQIMEKGSDKEISKYSVQMGAARFQLQQMGHHLFRDERSDPDPRVQYFIPDTWQRELLDVVDNNESALIVAPTSSGKTYASYYCMEKVLKESDEGVVVYVSPSKALINQVVATIYNQFTKSLPHGLVIYGVFTRDYRTDAMNCQILVTVPECLEILLLAPQHQQWAQKIRYVIFDEVHCLGGEIGADVWEHILVMIRCPFLALSATISNPKHLTEWLQLVKRYWQNVENTTESSVSANALKGSKKQKVQKAEKKSHRVRLVQYEERYNDLEKYVCSLKNDDFAIDHYHPFAALTVNHIEKYGIPSDLAFSPRESIQLYDVMADIWQEWPRAQELHPEEYSAFKNKVVITKADAKNYERELKKEITSWIELGHREKVNQLLEYLKPQVDDNPENEKVKFARFVEKLKEIDKLPAIFFMFQISSVRDFAKIVSQYLEEKQKNKQDSNVEKQEQYLKEKIKKLEKSMEKTRSALEKGSPSNKLESTIVTFENERSQLEEKLAKLTEIPPDCTFANSKAVDNETWTKIFSRLKYSANGKVLTDLALRGIGYHHEFMRIRERQVVEMLFRLGYIKVVTATGTLALGINMPCKSVVFPQDSVFLDSLNYRQMSGRAGRRGEDLIGNVFFYDIPLPKVGQLLKSNVPLLKGQFPLNISLVLRLMLLAAKADDKEDAKAKVLSVLQHPLMAFRKEKITELLKVYFIFSLQFLIKEGYLDQECNPRGFSELVASLHKHKPSNFVFVSFLERGLFHKLCKPTQEGSKVFSEDIMETLVLILANLFGRQYLPSSTVCQSEEFLGDLPEDFAAAVEEYNSKIQKNFGYFLLTVSKLADMKQECKLPLSGIDFAGTEFKDSELASHLLSGKESRTAVSPFACLSELVDQDLFDTSVVNDAILRTVGVSSSSAPVFYLKKFDEHGRRCPLSAYALDFYRQGSLEFLTQEMWDLLKNFTHLINSIRTSLEELCENEDDNVLLAFKQLSDSYSEKFSKV

Target ORF information:

RefSeq Version	XM_029076583.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus probable ATP-dependent RNA helicase DDX60 (LOC100077504), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029076583.1

ORF Insert Sequence:

ATGGCTTCTG GAGAAGCTGC GTTAGAGGAA GACGTCAATA ACGTTCAGAG TGACGCTGAT 
GAGGACAGTG AGCCCGAAGA AATTTTCTCA TCATTTGGTG ATTCTAAGGC ATCCGGTACA 
CCAGAATATG GAGGAAAATC CCAACCCGAC TCTGAAGCTG ATAGAGGAGA TTGTGAAGGC 
ACCGAGGCCA ATTCTGAGAT GCCAAATCCA GATCAATCAA AAGACATTAC CTTTCTGAAA 
AAATTGTCAT GTAACATTGA GGAGACTCTG TCACAAGCAA AGTACATCAG CTTACTTAAA 
GATTTTGTTG ACTCTGAATT TTTCTTGATT GATGGGGATT CATTATTTCT TACCGGTCTT 
TTCAACAAGA CTTTTAAATC AGGGCAACAT CTTCATTTCT TCTACTTGAT AGAACGCTTT 
CTGGATGATT TCTCTCAGAA AGGAGTGAAA TATGTCATTG TATTTTTTAA GGATGTAGAA 
AATATGTACC AGAGTCTCCA CACTCGTTTC TTACGTACTG CATTAATACA ACATCTAAAA 
CACAACACTG AGGTTACCAT AAGTACAGAT TTTTCCAATT GTTTATCACC TGAATGGGAG 
ATTTTCCTTA AAGAAAGTTT TCCATATTTC ATTATAGTCT CTGATGTAGG AATGACAAAA 
CAACAAACGG ACTATTTAAA TATATTCATC AGCCATACCC TTGGAAAGAA AATCAATGTG 
GTACTGACTT CAGGACAGGA ATCCGATATC CTTCGAGCTT ATGGGTACCA CATCCAGAGC 
TCACCGAAGC ACAGGAAATT CTTCAGTGAG CATCAGGAAG AGCTGCAAGA TGCTTGTGAG 
TCCATAATTC AGCATTTTTC CAAAACCCAG GATTCGGAAA TACATTTAAA GCAGCACTTG 
GATGCCATTC AGAAAGAGGT GCACCAAACA CTACCCCCAC TTAATAAACT GTGGCCAGAA 
GGAGCTGACA TTCGACGAAT TGTGTGCATT CTCTCATGCT CAGTGGGCTT GAAAACATAT 
GGTGACATGT TAAAGGATGC AGATGTCTTT GAAAAACTAA ATGAAAGCAC TGAAAACGAT 
AATCCTAAGG AAAGCAGAGA AGCACTGTCC CTGCAGGCTG TGGCCGACCT TTATAGGATG 
CATTGTCTCT GTGTGGCTCT TCTTCTGCAC CTGCCTCTTC CTCAGCGAGC TCGGAGCAGG 
ATCACAGGTT ATGTTTGGAA GAAAAGCCTG TCCCCATTTT TGAGAATGAA ATACAGATGT 
GAATTTGTCA TTCTAAAGCA GCTAAATGCT ATGAGCGACT GGAAAGTGGA TTTCACCTAC 
CTGCCTGACT TAAGTGATAA TTTGTTGTGG CAAAACATTG CCCACTATTA TGAAGTAGAC 
AGTTGCCAAG GATTAAATCT AGAGTTAGGG GATAAAATTT TAAAAGAATA TCAAAACTTA 
TGGAGCGTAG TATTAAAACT GTCAGGAAAT TGTGACTTTG GAGGACCAAT TCCTGTGAGA 
AACACTTCAG AGGCTTTCCT TAGAGAGAAA CCAGAAGCTG GAGAAACAAA AGAAGAGATT 
CCCAAGTTAG GGCTCATCCC AATGGAATCA TCCATAGTGA ACGATTATGC AGGAGATGTT 
CTGAAAAGTC TGCCTTTTCT AAGCAGTGAT GACCCGGCTA TCAATTCACT TATTAAGGAG 
AAGGAATTTG ATGAGCTGAC ACAATGGAAT TTCAGCCGAT CTCTCAGTGA TGATTATGAG 
AGAACAAAAC GCAGCCAGGA TGTAAATACT AAAGACCCTA AAGAACGAAG AGGAATCCAG 
AAGTTACAGT CCTTTTATCA ATTGCTTGGC CAGTCAATGC CAGGTAGTAG ATCGACAAGA 
ATTGTAAGCC AGGTGGCTGT AGCCACCAAA GCAAACCCTG CTGACAAAAA GAAAGCAAAA 
TTACCTAGGA AAAAAGCAGA CATAATTGCC GAAGAAAATC TGAAAGTACA GAAAGCCAAA 
GTGGAAAATA AAGAGCAGGA GCAGTGGAGT ACCCTGTGTG GATCTATTGA AAAGGAAATT 
AAGACAAATT TTGCTGGTGG ATTAAAACGG TTGGAGACTT TTCTCAAGAC ATGCCAAAGC 
CAGTCGGTAA AATTTCTCGC TGAGGTGGTA GGATTGGATG CCTGCTTCAA AGCATGGGTG 
GAGCACTGCC GAAAATCAGA AGAAAAATCA AAAGATTTGG ACATAGCAGT GCAGGTGATG 
CAAAGGATCC ATACAATCTA TGGAAAATAT CAAGAACACT TACAGAAGAC ACATCTACAA 
AAACTGGCTA AATATCTAAA ATACCTTGGA TTTGACAACC TGGCATGCTC ATTATCTTCT 
CAGATAATGG AGAAAGGCAG CGACAAGGAG ATTTCAAAGT ACTCTGTTCA GATGGGAGCT 
GCTCGATTTC AGCTGCAACA GATGGGGCAT CATTTGTTCA GGGATGAGAG ATCGGATCCG 
GACCCCAGAG TACAGTATTT TATACCAGAC ACGTGGCAGC GAGAACTTCT CGATGTCGTG 
GACAATAATG AGTCTGCTTT GATCGTTGCT CCAACTTCAT CTGGTAAAAC ATACGCCTCA 
TACTACTGCA TGGAGAAAGT GCTGAAAGAG AGCGATGAAG GGGTCGTTGT CTATGTTTCT 
CCATCAAAGG CTCTTATAAA TCAAGTGGTC GCAACTATAT ATAACCAGTT TACCAAAAGC 
CTGCCTCACG GATTAGTAAT ATATGGAGTT TTCACGAGGG ATTACCGCAC AGATGCCATG 
AACTGCCAGA TTCTCGTGAC GGTACCTGAG TGTTTAGAAA TCCTGCTGCT AGCCCCTCAA 
CACCAGCAAT GGGCTCAGAA GATCAGATAT GTTATATTTG ATGAGGTCCA TTGTCTAGGC 
GGAGAAATCG GAGCAGACGT TTGGGAACAC ATCCTTGTTA TGATCCGCTG CCCCTTTTTG 
GCTCTGTCTG CAACCATAAG TAACCCAAAG CACCTCACTG AGTGGTTGCA GTTGGTGAAA 
AGGTACTGGC AGAATGTGGA AAACACAACG GAAAGCTCTG TATCTGCAAA TGCCCTAAAA 
GGTTCAAAGA AACAAAAGGT TCAAAAGGCA GAGAAGAAAT CACATAGAGT TCGACTTGTA 
CAGTATGAAG AGAGGTACAA TGATCTAGAA AAATACGTGT GCTCTCTGAA GAATGATGAC 
TTTGCCATCG ATCACTATCA TCCATTTGCC GCATTAACTG TCAATCATAT TGAAAAATAT 
GGAATTCCCT CAGACCTTGC ATTTTCCCCA CGAGAAAGCA TTCAACTCTA TGACGTCATG 
GCAGATATTT GGCAGGAATG GCCCAGAGCA CAGGAACTGC ATCCTGAAGA ATACAGTGCT 
TTCAAGAATA AAGTAGTGAT CACTAAAGCA GATGCAAAAA ATTATGAAAG GGAACTGAAG 
AAAGAAATAA CCAGCTGGAT TGAGCTTGGC CATAGAGAAA AGGTGAACCA ACTATTGGAG 
TACCTTAAAC CTCAAGTCGA TGATAATCCG GAAAATGAAA AAGTAAAGTT TGCACGTTTT 
GTTGAGAAGC TAAAAGAGAT TGATAAATTG CCTGCAATTT TTTTTATGTT CCAAATCTCT 
TCAGTACGTG ATTTTGCCAA GATTGTAAGT CAGTATTTGG AAGAGAAACA GAAGAATAAA 
CAAGATTCAA ATGTTGAGAA ACAAGAACAA TATTTAAAAG AAAAAATTAA GAAGTTGGAA 
AAGTCCATGG AAAAAACAAG ATCAGCATTG GAGAAAGGAA GTCCCAGCAA TAAACTCGAG 
AGCACCATTG TTACTTTCGA GAATGAAAGA AGTCAATTAG AGGAGAAACT TGCTAAACTT 
ACTGAAATTC CTCCGGACTG TACTTTCGCT AACAGTAAAG CAGTAGATAA TGAAACCTGG 
ACCAAAATCT TTAGTCGACT CAAGTATTCG GCAAATGGCA AAGTACTGAC TGATCTGGCA 
CTCCGTGGCA TCGGCTACCA TCATGAATTC ATGAGAATCC GAGAGAGACA AGTGGTGGAG 
ATGCTCTTCA GGCTGGGATA TATCAAGGTT GTGACGGCTA CTGGAACCCT GGCTTTGGGT 
ATTAACATGC CGTGCAAATC TGTTGTCTTC CCTCAAGATT CCGTCTTTCT GGATTCCCTC 
AATTACAGAC AGATGTCCGG GCGTGCAGGA AGACGAGGCG AAGACCTGAT AGGAAATGTC 
TTCTTCTACG ATATTCCGTT GCCCAAAGTT GGGCAGCTGT TGAAGTCTAA CGTCCCGCTG 
CTAAAAGGCC AGTTCCCTCT GAACATTTCC CTGGTCCTGC GACTCATGCT TCTGGCCGCA 
AAGGCCGATG ACAAGGAGGA TGCCAAGGCC AAGGTGTTGT CTGTCCTGCA GCATCCATTG 
ATGGCCTTCA GAAAAGAGAA GATCACAGAA CTGTTAAAAG TGTACTTTAT ATTTTCCTTG 
CAGTTTCTTA TTAAAGAGGG ATATCTGGAT CAAGAATGCA ACCCCAGGGG ATTTTCTGAA 
CTGGTGGCCA GTTTGCACAA ACACAAGCCT TCAAATTTTG TGTTCGTGAG CTTTCTCGAG 
AGGGGCTTAT TTCACAAGTT GTGTAAGCCA ACCCAAGAGG GCTCAAAGGT TTTCTCTGAA 
GATATAATGG AAACACTAGT GCTCATCCTA GCAAATCTTT TTGGAAGACA ATATTTGCCG 
TCCTCGACGG TTTGCCAATC AGAGGAGTTC CTAGGCGATC TCCCGGAAGA CTTCGCCGCC 
GCAGTTGAGG AGTACAATAG CAAAATCCAG AAGAACTTTG GCTATTTCCT GCTTACAGTC 
TCCAAACTGG CTGACATGAA ACAAGAATGC AAGCTGCCTC TGTCGGGAAT TGATTTTGCA 
GGTACAGAAT TCAAGGACTC GGAACTGGCA TCTCATTTAC TGAGCGGCAA GGAGAGCAGA 
ACCGCTGTCT CGCCTTTCGC TTGCCTCTCT GAACTGGTGG ACCAGGATCT CTTTGATACC 
AGTGTGGTAA ACGATGCGAT CCTGCGAACG GTGGGTGTAA GTTCTTCCAG CGCTCCTGTG 
TTCTATTTGA AGAAATTTGA TGAACACGGG AGAAGATGCC CACTCAGTGC TTATGCCCTT 
GACTTCTATA GACAGGGCTC CCTGGAATTC CTGACTCAGG AGATGTGGGA TCTGCTCAAA 
AACTTTACAC ATCTTATCAA TTCCATCAGG ACATCTTTAG AAGAATTGTG TGAGAACGAG 
GATGACAATG TTCTGCTGGC ATTTAAACAA CTGAGCGACA GCTACAGTGA AAAGTTTAGC 
AAAGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100077504 ( XM_029076584.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028932417.1 Ornithorhynchus anatinus probable ATP-dependent RNA helicase DDX60 (LOC100077504), transcript variant X2, mRNA.

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CloneID	OOc114082
Clone ID Related Accession (Same CDS sequence)	XM_029076584.1
Accession Version	XM_029076584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556380800000
Organism	Ornithorhynchus anatinus(platypus)
Product	probable ATP-dependent RNA helicase DDX60 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTG GAGAAGCTGC GTTAGAGGAA GACGTCAATA ACGTTCAGAG TGACGCTGAT 
GAGGACAGTG AGCCCGAAGA AATTTTCTCA TCATTTGGTG ATTCTAAGGC ATCCGGTACA 
CCAGAATATG GAGGAAAATC CCAACCCGAC TCTGAAGCTG ATAGAGGAGA TTGTGAAGGC 
ACCGAGGCCA ATTCTGAGAT GCCAAATCCA GATCAATCAA AAGACATTAC CTTTCTGAAA 
AAATTGTCAT GTAACATTGA GGAGACTCTG TCACAAGCAA AGTACATCAG CTTACTTAAA 
GATTTTGTTG ACTCTGAATT TTTCTTGATT GATGGGGATT CATTATTTCT TACCGGTCTT 
TTCAACAAGA CTTTTAAATC AGGGCAACAT CTTCATTTCT TCTACTTGAT AGAACGCTTT 
CTGGATGATT TCTCTCAGAA AGGAGTGAAA TATGTCATTG TATTTTTTAA GGATGTAGAA 
AATATGTACC AGAGTCTCCA CACTCGTTTC TTACGTACTG CATTAATACA ACATCTAAAA 
CACAACACTG AGGTTACCAT AAGTACAGAT TTTTCCAATT GTTTATCACC TGAATGGGAG 
ATTTTCCTTA AAGAAAGTTT TCCATATTTC ATTATAGTCT CTGATGTAGG AATGACAAAA 
CAACAAACGG ACTATTTAAA TATATTCATC AGCCATACCC TTGGAAAGAA AATCAATGTG 
GTACTGACTT CAGGACAGGA ATCCGATATC CTTCGAGCTT ATGGGTACCA CATCCAGAGC 
TCACCGAAGC ACAGGAAATT CTTCAGTGAG CATCAGGAAG AGCTGCAAGA TGCTTGTGAG 
TCCATAATTC AGCATTTTTC CAAAACCCAG GATTCGGAAA TACATTTAAA GCAGCACTTG 
GATGCCATTC AGAAAGAGGT GCACCAAACA CTACCCCCAC TTAATAAACT GTGGCCAGAA 
GGAGCTGACA TTCGACGAAT TGTGTGCATT CTCTCATGCT CAGTGGGCTT GAAAACATAT 
GGTGACATGT TAAAGGATGC AGATGTCTTT GAAAAACTAA ATGAAAGCAC TGAAAACGAT 
AATCCTAAGG AAAGCAGAGA AGCACTGTCC CTGCAGGCTG TGGCCGACCT TTATAGGATG 
CATTGTCTCT GTGTGGCTCT TCTTCTGCAC CTGCCTCTTC CTCAGCGAGC TCGGAGCAGG 
ATCACAGGTT ATGTTTGGAA GAAAAGCCTG TCCCCATTTT TGAGAATGAA ATACAGATGT 
GAATTTGTCA TTCTAAAGCA GCTAAATGCT ATGAGCGACT GGAAAGTGGA TTTCACCTAC 
CTGCCTGACT TAAGTGATAA TTTGTTGTGG CAAAACATTG CCCACTATTA TGAAGTAGAC 
AGTTGCCAAG GATTAAATCT AGAGTTAGGG GATAAAATTT TAAAAGAATA TCAAAACTTA 
TGGAGCGTAG TATTAAAACT GTCAGGAAAT TGTGACTTTG GAGGACCAAT TCCTGTGAGA 
AACACTTCAG AGGCTTTCCT TAGAGAGAAA CCAGAAGCTG GAGAAACAAA AGAAGAGATT 
CCCAAGTTAG GGCTCATCCC AATGGAATCA TCCATAGTGA ACGATTATGC AGGAGATGTT 
CTGAAAAGTC TGCCTTTTCT AAGCAGTGAT GACCCGGCTA TCAATTCACT TATTAAGGAG 
AAGGAATTTG ATGAGCTGAC ACAATGGAAT TTCAGCCGAT CTCTCAGTGA TGATTATGAG 
AGAACAAAAC GCAGCCAGGA TGTAAATACT AAAGACCCTA AAGAACGAAG AGGAATCCAG 
AAGTTACAGT CCTTTTATCA ATTGCTTGGC CAGTCAATGC CAGGTAGTAG ATCGACAAGA 
ATTGTAAGCC AGGTGGCTGT AGCCACCAAA GCAAACCCTG CTGACAAAAA GAAAGCAAAA 
TTACCTAGGA AAAAAGCAGA CATAATTGCC GAAGAAAATC TGAAAGTACA GAAAGCCAAA 
GTGGAAAATA AAGAGCAGGA GCAGTGGAGT ACCCTGTGTG GATCTATTGA AAAGGAAATT 
AAGACAAATT TTGCTGGTGG ATTAAAACGG TTGGAGACTT TTCTCAAGAC ATGCCAAAGC 
CAGTCGGTAA AATTTCTCGC TGAGGTGGTA GGATTGGATG CCTGCTTCAA AGCATGGGTG 
GAGCACTGCC GAAAATCAGA AAAATCAAAA GATTTGGACA TAGCAGTGCA GGTGATGCAA 
AGGATCCATA CAATCTATGG AAAATATCAA GAACACTTAC AGAAGACACA TCTACAAAAA 
CTGGCTAAAT ATCTAAAATA CCTTGGATTT GACAACCTGG CATGCTCATT ATCTTCTCAG 
ATAATGGAGA AAGGCAGCGA CAAGGAGATT TCAAAGTACT CTGTTCAGAT GGGAGCTGCT 
CGATTTCAGC TGCAACAGAT GGGGCATCAT TTGTTCAGGG ATGAGAGATC GGATCCGGAC 
CCCAGAGTAC AGTATTTTAT ACCAGACACG TGGCAGCGAG AACTTCTCGA TGTCGTGGAC 
AATAATGAGT CTGCTTTGAT CGTTGCTCCA ACTTCATCTG GTAAAACATA CGCCTCATAC 
TACTGCATGG AGAAAGTGCT GAAAGAGAGC GATGAAGGGG TCGTTGTCTA TGTTTCTCCA 
TCAAAGGCTC TTATAAATCA AGTGGTCGCA ACTATATATA ACCAGTTTAC CAAAAGCCTG 
CCTCACGGAT TAGTAATATA TGGAGTTTTC ACGAGGGATT ACCGCACAGA TGCCATGAAC 
TGCCAGATTC TCGTGACGGT ACCTGAGTGT TTAGAAATCC TGCTGCTAGC CCCTCAACAC 
CAGCAATGGG CTCAGAAGAT CAGATATGTT ATATTTGATG AGGTCCATTG TCTAGGCGGA 
GAAATCGGAG CAGACGTTTG GGAACACATC CTTGTTATGA TCCGCTGCCC CTTTTTGGCT 
CTGTCTGCAA CCATAAGTAA CCCAAAGCAC CTCACTGAGT GGTTGCAGTT GGTGAAAAGG 
TACTGGCAGA ATGTGGAAAA CACAACGGAA AGCTCTGTAT CTGCAAATGC CCTAAAAGGT 
TCAAAGAAAC AAAAGGTTCA AAAGGCAGAG AAGAAATCAC ATAGAGTTCG ACTTGTACAG 
TATGAAGAGA GGTACAATGA TCTAGAAAAA TACGTGTGCT CTCTGAAGAA TGATGACTTT 
GCCATCGATC ACTATCATCC ATTTGCCGCA TTAACTGTCA ATCATATTGA AAAATATGGA 
ATTCCCTCAG ACCTTGCATT TTCCCCACGA GAAAGCATTC AACTCTATGA CGTCATGGCA 
GATATTTGGC AGGAATGGCC CAGAGCACAG GAACTGCATC CTGAAGAATA CAGTGCTTTC 
AAGAATAAAG TAGTGATCAC TAAAGCAGAT GCAAAAAATT ATGAAAGGGA ACTGAAGAAA 
GAAATAACCA GCTGGATTGA GCTTGGCCAT AGAGAAAAGG TGAACCAACT ATTGGAGTAC 
CTTAAACCTC AAGTCGATGA TAATCCGGAA AATGAAAAAG TAAAGTTTGC ACGTTTTGTT 
GAGAAGCTAA AAGAGATTGA TAAATTGCCT GCAATTTTTT TTATGTTCCA AATCTCTTCA 
GTACGTGATT TTGCCAAGAT TGTAAGTCAG TATTTGGAAG AGAAACAGAA GAATAAACAA 
GATTCAAATG TTGAGAAACA AGAACAATAT TTAAAAGAAA AAATTAAGAA GTTGGAAAAG 
TCCATGGAAA AAACAAGATC AGCATTGGAG AAAGGAAGTC CCAGCAATAA ACTCGAGAGC 
ACCATTGTTA CTTTCGAGAA TGAAAGAAGT CAATTAGAGG AGAAACTTGC TAAACTTACT 
GAAATTCCTC CGGACTGTAC TTTCGCTAAC AGTAAAGCAG TAGATAATGA AACCTGGACC 
AAAATCTTTA GTCGACTCAA GTATTCGGCA AATGGCAAAG TACTGACTGA TCTGGCACTC 
CGTGGCATCG GCTACCATCA TGAATTCATG AGAATCCGAG AGAGACAAGT GGTGGAGATG 
CTCTTCAGGC TGGGATATAT CAAGGTTGTG ACGGCTACTG GAACCCTGGC TTTGGGTATT 
AACATGCCGT GCAAATCTGT TGTCTTCCCT CAAGATTCCG TCTTTCTGGA TTCCCTCAAT 
TACAGACAGA TGTCCGGGCG TGCAGGAAGA CGAGGCGAAG ACCTGATAGG AAATGTCTTC 
TTCTACGATA TTCCGTTGCC CAAAGTTGGG CAGCTGTTGA AGTCTAACGT CCCGCTGCTA 
AAAGGCCAGT TCCCTCTGAA CATTTCCCTG GTCCTGCGAC TCATGCTTCT GGCCGCAAAG 
GCCGATGACA AGGAGGATGC CAAGGCCAAG GTGTTGTCTG TCCTGCAGCA TCCATTGATG 
GCCTTCAGAA AAGAGAAGAT CACAGAACTG TTAAAAGTGT ACTTTATATT TTCCTTGCAG 
TTTCTTATTA AAGAGGGATA TCTGGATCAA GAATGCAACC CCAGGGGATT TTCTGAACTG 
GTGGCCAGTT TGCACAAACA CAAGCCTTCA AATTTTGTGT TCGTGAGCTT TCTCGAGAGG 
GGCTTATTTC ACAAGTTGTG TAAGCCAACC CAAGAGGGCT CAAAGGTTTT CTCTGAAGAT 
ATAATGGAAA CACTAGTGCT CATCCTAGCA AATCTTTTTG GAAGACAATA TTTGCCGTCC 
TCGACGGTTT GCCAATCAGA GGAGTTCCTA GGCGATCTCC CGGAAGACTT CGCCGCCGCA 
GTTGAGGAGT ACAATAGCAA AATCCAGAAG AACTTTGGCT ATTTCCTGCT TACAGTCTCC 
AAACTGGCTG ACATGAAACA AGAATGCAAG CTGCCTCTGT CGGGAATTGA TTTTGCAGGT 
ACAGAATTCA AGGACTCGGA ACTGGCATCT CATTTACTGA GCGGCAAGGA GAGCAGAACC 
GCTGTCTCGC CTTTCGCTTG CCTCTCTGAA CTGGTGGACC AGGATCTCTT TGATACCAGT 
GTGGTAAACG ATGCGATCCT GCGAACGGTG GGTGTAAGTT CTTCCAGCGC TCCTGTGTTC 
TATTTGAAGA AATTTGATGA ACACGGGAGA AGATGCCCAC TCAGTGCTTA TGCCCTTGAC 
TTCTATAGAC AGGGCTCCCT GGAATTCCTG ACTCAGGAGA TGTGGGATCT GCTCAAAAAC 
TTTACACATC TTATCAATTC CATCAGGACA TCTTTAGAAG AATTGTGTGA GAACGAGGAT 
GACAATGTTC TGCTGGCATT TAAACAACTG AGCGACAGCT ACAGTGAAAA GTTTAGCAAA 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028932417.1
CDS	114..5399
Translation	MASGEAALEEDVNNVQSDADEDSEPEEIFSSFGDSKASGTPEYGGKSQPDSEADRGDCEGTEANSEMPNPDQSKDITFLKKLSCNIEETLSQAKYISLLKDFVDSEFFLIDGDSLFLTGLFNKTFKSGQHLHFFYLIERFLDDFSQKGVKYVIVFFKDVENMYQSLHTRFLRTALIQHLKHNTEVTISTDFSNCLSPEWEIFLKESFPYFIIVSDVGMTKQQTDYLNIFISHTLGKKINVVLTSGQESDILRAYGYHIQSSPKHRKFFSEHQEELQDACESIIQHFSKTQDSEIHLKQHLDAIQKEVHQTLPPLNKLWPEGADIRRIVCILSCSVGLKTYGDMLKDADVFEKLNESTENDNPKESREALSLQAVADLYRMHCLCVALLLHLPLPQRARSRITGYVWKKSLSPFLRMKYRCEFVILKQLNAMSDWKVDFTYLPDLSDNLLWQNIAHYYEVDSCQGLNLELGDKILKEYQNLWSVVLKLSGNCDFGGPIPVRNTSEAFLREKPEAGETKEEIPKLGLIPMESSIVNDYAGDVLKSLPFLSSDDPAINSLIKEKEFDELTQWNFSRSLSDDYERTKRSQDVNTKDPKERRGIQKLQSFYQLLGQSMPGSRSTRIVSQVAVATKANPADKKKAKLPRKKADIIAEENLKVQKAKVENKEQEQWSTLCGSIEKEIKTNFAGGLKRLETFLKTCQSQSVKFLAEVVGLDACFKAWVEHCRKSEKSKDLDIAVQVMQRIHTIYGKYQEHLQKTHLQKLAKYLKYLGFDNLACSLSSQIMEKGSDKEISKYSVQMGAARFQLQQMGHHLFRDERSDPDPRVQYFIPDTWQRELLDVVDNNESALIVAPTSSGKTYASYYCMEKVLKESDEGVVVYVSPSKALINQVVATIYNQFTKSLPHGLVIYGVFTRDYRTDAMNCQILVTVPECLEILLLAPQHQQWAQKIRYVIFDEVHCLGGEIGADVWEHILVMIRCPFLALSATISNPKHLTEWLQLVKRYWQNVENTTESSVSANALKGSKKQKVQKAEKKSHRVRLVQYEERYNDLEKYVCSLKNDDFAIDHYHPFAALTVNHIEKYGIPSDLAFSPRESIQLYDVMADIWQEWPRAQELHPEEYSAFKNKVVITKADAKNYERELKKEITSWIELGHREKVNQLLEYLKPQVDDNPENEKVKFARFVEKLKEIDKLPAIFFMFQISSVRDFAKIVSQYLEEKQKNKQDSNVEKQEQYLKEKIKKLEKSMEKTRSALEKGSPSNKLESTIVTFENERSQLEEKLAKLTEIPPDCTFANSKAVDNETWTKIFSRLKYSANGKVLTDLALRGIGYHHEFMRIRERQVVEMLFRLGYIKVVTATGTLALGINMPCKSVVFPQDSVFLDSLNYRQMSGRAGRRGEDLIGNVFFYDIPLPKVGQLLKSNVPLLKGQFPLNISLVLRLMLLAAKADDKEDAKAKVLSVLQHPLMAFRKEKITELLKVYFIFSLQFLIKEGYLDQECNPRGFSELVASLHKHKPSNFVFVSFLERGLFHKLCKPTQEGSKVFSEDIMETLVLILANLFGRQYLPSSTVCQSEEFLGDLPEDFAAAVEEYNSKIQKNFGYFLLTVSKLADMKQECKLPLSGIDFAGTEFKDSELASHLLSGKESRTAVSPFACLSELVDQDLFDTSVVNDAILRTVGVSSSSAPVFYLKKFDEHGRRCPLSAYALDFYRQGSLEFLTQEMWDLLKNFTHLINSIRTSLEELCENEDDNVLLAFKQLSDSYSEKFSKV

Target ORF information:

RefSeq Version	XM_029076584.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus probable ATP-dependent RNA helicase DDX60 (LOC100077504), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029076584.1

ORF Insert Sequence:

ATGGCTTCTG GAGAAGCTGC GTTAGAGGAA GACGTCAATA ACGTTCAGAG TGACGCTGAT 
GAGGACAGTG AGCCCGAAGA AATTTTCTCA TCATTTGGTG ATTCTAAGGC ATCCGGTACA 
CCAGAATATG GAGGAAAATC CCAACCCGAC TCTGAAGCTG ATAGAGGAGA TTGTGAAGGC 
ACCGAGGCCA ATTCTGAGAT GCCAAATCCA GATCAATCAA AAGACATTAC CTTTCTGAAA 
AAATTGTCAT GTAACATTGA GGAGACTCTG TCACAAGCAA AGTACATCAG CTTACTTAAA 
GATTTTGTTG ACTCTGAATT TTTCTTGATT GATGGGGATT CATTATTTCT TACCGGTCTT 
TTCAACAAGA CTTTTAAATC AGGGCAACAT CTTCATTTCT TCTACTTGAT AGAACGCTTT 
CTGGATGATT TCTCTCAGAA AGGAGTGAAA TATGTCATTG TATTTTTTAA GGATGTAGAA 
AATATGTACC AGAGTCTCCA CACTCGTTTC TTACGTACTG CATTAATACA ACATCTAAAA 
CACAACACTG AGGTTACCAT AAGTACAGAT TTTTCCAATT GTTTATCACC TGAATGGGAG 
ATTTTCCTTA AAGAAAGTTT TCCATATTTC ATTATAGTCT CTGATGTAGG AATGACAAAA 
CAACAAACGG ACTATTTAAA TATATTCATC AGCCATACCC TTGGAAAGAA AATCAATGTG 
GTACTGACTT CAGGACAGGA ATCCGATATC CTTCGAGCTT ATGGGTACCA CATCCAGAGC 
TCACCGAAGC ACAGGAAATT CTTCAGTGAG CATCAGGAAG AGCTGCAAGA TGCTTGTGAG 
TCCATAATTC AGCATTTTTC CAAAACCCAG GATTCGGAAA TACATTTAAA GCAGCACTTG 
GATGCCATTC AGAAAGAGGT GCACCAAACA CTACCCCCAC TTAATAAACT GTGGCCAGAA 
GGAGCTGACA TTCGACGAAT TGTGTGCATT CTCTCATGCT CAGTGGGCTT GAAAACATAT 
GGTGACATGT TAAAGGATGC AGATGTCTTT GAAAAACTAA ATGAAAGCAC TGAAAACGAT 
AATCCTAAGG AAAGCAGAGA AGCACTGTCC CTGCAGGCTG TGGCCGACCT TTATAGGATG 
CATTGTCTCT GTGTGGCTCT TCTTCTGCAC CTGCCTCTTC CTCAGCGAGC TCGGAGCAGG 
ATCACAGGTT ATGTTTGGAA GAAAAGCCTG TCCCCATTTT TGAGAATGAA ATACAGATGT 
GAATTTGTCA TTCTAAAGCA GCTAAATGCT ATGAGCGACT GGAAAGTGGA TTTCACCTAC 
CTGCCTGACT TAAGTGATAA TTTGTTGTGG CAAAACATTG CCCACTATTA TGAAGTAGAC 
AGTTGCCAAG GATTAAATCT AGAGTTAGGG GATAAAATTT TAAAAGAATA TCAAAACTTA 
TGGAGCGTAG TATTAAAACT GTCAGGAAAT TGTGACTTTG GAGGACCAAT TCCTGTGAGA 
AACACTTCAG AGGCTTTCCT TAGAGAGAAA CCAGAAGCTG GAGAAACAAA AGAAGAGATT 
CCCAAGTTAG GGCTCATCCC AATGGAATCA TCCATAGTGA ACGATTATGC AGGAGATGTT 
CTGAAAAGTC TGCCTTTTCT AAGCAGTGAT GACCCGGCTA TCAATTCACT TATTAAGGAG 
AAGGAATTTG ATGAGCTGAC ACAATGGAAT TTCAGCCGAT CTCTCAGTGA TGATTATGAG 
AGAACAAAAC GCAGCCAGGA TGTAAATACT AAAGACCCTA AAGAACGAAG AGGAATCCAG 
AAGTTACAGT CCTTTTATCA ATTGCTTGGC CAGTCAATGC CAGGTAGTAG ATCGACAAGA 
ATTGTAAGCC AGGTGGCTGT AGCCACCAAA GCAAACCCTG CTGACAAAAA GAAAGCAAAA 
TTACCTAGGA AAAAAGCAGA CATAATTGCC GAAGAAAATC TGAAAGTACA GAAAGCCAAA 
GTGGAAAATA AAGAGCAGGA GCAGTGGAGT ACCCTGTGTG GATCTATTGA AAAGGAAATT 
AAGACAAATT TTGCTGGTGG ATTAAAACGG TTGGAGACTT TTCTCAAGAC ATGCCAAAGC 
CAGTCGGTAA AATTTCTCGC TGAGGTGGTA GGATTGGATG CCTGCTTCAA AGCATGGGTG 
GAGCACTGCC GAAAATCAGA AAAATCAAAA GATTTGGACA TAGCAGTGCA GGTGATGCAA 
AGGATCCATA CAATCTATGG AAAATATCAA GAACACTTAC AGAAGACACA TCTACAAAAA 
CTGGCTAAAT ATCTAAAATA CCTTGGATTT GACAACCTGG CATGCTCATT ATCTTCTCAG 
ATAATGGAGA AAGGCAGCGA CAAGGAGATT TCAAAGTACT CTGTTCAGAT GGGAGCTGCT 
CGATTTCAGC TGCAACAGAT GGGGCATCAT TTGTTCAGGG ATGAGAGATC GGATCCGGAC 
CCCAGAGTAC AGTATTTTAT ACCAGACACG TGGCAGCGAG AACTTCTCGA TGTCGTGGAC 
AATAATGAGT CTGCTTTGAT CGTTGCTCCA ACTTCATCTG GTAAAACATA CGCCTCATAC 
TACTGCATGG AGAAAGTGCT GAAAGAGAGC GATGAAGGGG TCGTTGTCTA TGTTTCTCCA 
TCAAAGGCTC TTATAAATCA AGTGGTCGCA ACTATATATA ACCAGTTTAC CAAAAGCCTG 
CCTCACGGAT TAGTAATATA TGGAGTTTTC ACGAGGGATT ACCGCACAGA TGCCATGAAC 
TGCCAGATTC TCGTGACGGT ACCTGAGTGT TTAGAAATCC TGCTGCTAGC CCCTCAACAC 
CAGCAATGGG CTCAGAAGAT CAGATATGTT ATATTTGATG AGGTCCATTG TCTAGGCGGA 
GAAATCGGAG CAGACGTTTG GGAACACATC CTTGTTATGA TCCGCTGCCC CTTTTTGGCT 
CTGTCTGCAA CCATAAGTAA CCCAAAGCAC CTCACTGAGT GGTTGCAGTT GGTGAAAAGG 
TACTGGCAGA ATGTGGAAAA CACAACGGAA AGCTCTGTAT CTGCAAATGC CCTAAAAGGT 
TCAAAGAAAC AAAAGGTTCA AAAGGCAGAG AAGAAATCAC ATAGAGTTCG ACTTGTACAG 
TATGAAGAGA GGTACAATGA TCTAGAAAAA TACGTGTGCT CTCTGAAGAA TGATGACTTT 
GCCATCGATC ACTATCATCC ATTTGCCGCA TTAACTGTCA ATCATATTGA AAAATATGGA 
ATTCCCTCAG ACCTTGCATT TTCCCCACGA GAAAGCATTC AACTCTATGA CGTCATGGCA 
GATATTTGGC AGGAATGGCC CAGAGCACAG GAACTGCATC CTGAAGAATA CAGTGCTTTC 
AAGAATAAAG TAGTGATCAC TAAAGCAGAT GCAAAAAATT ATGAAAGGGA ACTGAAGAAA 
GAAATAACCA GCTGGATTGA GCTTGGCCAT AGAGAAAAGG TGAACCAACT ATTGGAGTAC 
CTTAAACCTC AAGTCGATGA TAATCCGGAA AATGAAAAAG TAAAGTTTGC ACGTTTTGTT 
GAGAAGCTAA AAGAGATTGA TAAATTGCCT GCAATTTTTT TTATGTTCCA AATCTCTTCA 
GTACGTGATT TTGCCAAGAT TGTAAGTCAG TATTTGGAAG AGAAACAGAA GAATAAACAA 
GATTCAAATG TTGAGAAACA AGAACAATAT TTAAAAGAAA AAATTAAGAA GTTGGAAAAG 
TCCATGGAAA AAACAAGATC AGCATTGGAG AAAGGAAGTC CCAGCAATAA ACTCGAGAGC 
ACCATTGTTA CTTTCGAGAA TGAAAGAAGT CAATTAGAGG AGAAACTTGC TAAACTTACT 
GAAATTCCTC CGGACTGTAC TTTCGCTAAC AGTAAAGCAG TAGATAATGA AACCTGGACC 
AAAATCTTTA GTCGACTCAA GTATTCGGCA AATGGCAAAG TACTGACTGA TCTGGCACTC 
CGTGGCATCG GCTACCATCA TGAATTCATG AGAATCCGAG AGAGACAAGT GGTGGAGATG 
CTCTTCAGGC TGGGATATAT CAAGGTTGTG ACGGCTACTG GAACCCTGGC TTTGGGTATT 
AACATGCCGT GCAAATCTGT TGTCTTCCCT CAAGATTCCG TCTTTCTGGA TTCCCTCAAT 
TACAGACAGA TGTCCGGGCG TGCAGGAAGA CGAGGCGAAG ACCTGATAGG AAATGTCTTC 
TTCTACGATA TTCCGTTGCC CAAAGTTGGG CAGCTGTTGA AGTCTAACGT CCCGCTGCTA 
AAAGGCCAGT TCCCTCTGAA CATTTCCCTG GTCCTGCGAC TCATGCTTCT GGCCGCAAAG 
GCCGATGACA AGGAGGATGC CAAGGCCAAG GTGTTGTCTG TCCTGCAGCA TCCATTGATG 
GCCTTCAGAA AAGAGAAGAT CACAGAACTG TTAAAAGTGT ACTTTATATT TTCCTTGCAG 
TTTCTTATTA AAGAGGGATA TCTGGATCAA GAATGCAACC CCAGGGGATT TTCTGAACTG 
GTGGCCAGTT TGCACAAACA CAAGCCTTCA AATTTTGTGT TCGTGAGCTT TCTCGAGAGG 
GGCTTATTTC ACAAGTTGTG TAAGCCAACC CAAGAGGGCT CAAAGGTTTT CTCTGAAGAT 
ATAATGGAAA CACTAGTGCT CATCCTAGCA AATCTTTTTG GAAGACAATA TTTGCCGTCC 
TCGACGGTTT GCCAATCAGA GGAGTTCCTA GGCGATCTCC CGGAAGACTT CGCCGCCGCA 
GTTGAGGAGT ACAATAGCAA AATCCAGAAG AACTTTGGCT ATTTCCTGCT TACAGTCTCC 
AAACTGGCTG ACATGAAACA AGAATGCAAG CTGCCTCTGT CGGGAATTGA TTTTGCAGGT 
ACAGAATTCA AGGACTCGGA ACTGGCATCT CATTTACTGA GCGGCAAGGA GAGCAGAACC 
GCTGTCTCGC CTTTCGCTTG CCTCTCTGAA CTGGTGGACC AGGATCTCTT TGATACCAGT 
GTGGTAAACG ATGCGATCCT GCGAACGGTG GGTGTAAGTT CTTCCAGCGC TCCTGTGTTC 
TATTTGAAGA AATTTGATGA ACACGGGAGA AGATGCCCAC TCAGTGCTTA TGCCCTTGAC 
TTCTATAGAC AGGGCTCCCT GGAATTCCTG ACTCAGGAGA TGTGGGATCT GCTCAAAAAC 
TTTACACATC TTATCAATTC CATCAGGACA TCTTTAGAAG AATTGTGTGA GAACGAGGAT 
GACAATGTTC TGCTGGCATT TAAACAACTG AGCGACAGCT ACAGTGAAAA GTTTAGCAAA 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100077504 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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