LOC101174648 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101174648
Entrez Gene ID	101174648
Full Name	mitogen-activated protein kinase kinase kinase kinase 4-like
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023965947.1	XP_023821715.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
XM_023965951.1	XP_023821719.1	traf2 and NCK-interacting protein kinase isoform X6
XM_023965953.1	XP_023821721.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
XM_020709038.1	XP_020564697.1	mitogen-activated protein kinase kinase kinase kinase 4-like
XM_023965937.1	XP_023821705.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
XM_023965940.1	XP_023821708.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
XM_023965931.1	XP_023821699.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
XM_023965943.1	XP_023821711.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC101174648 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101174648 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa112413	XM_023965931.1 Latest version!	Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OJa112414	XM_023965937.1 Latest version!	Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OJa112415	XM_023965940.1 Latest version!	Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OJa112416	XM_023965943.1 Latest version!	Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OJa112417	XM_023965947.1 Latest version!	Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OJa112418	XM_023965951.1 Latest version!	Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OJa112419	XM_023965953.1 Latest version!	Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OJa74677	XM_020709038.1 Latest version!	Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4-like (LOC101174648), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101174648 ( XM_023965931.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023821699.1 Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OJa112413
Clone ID Related Accession (Same CDS sequence)	XM_023965931.1
Accession Version	XM_023965931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCAGC TAAAAGTCTG GTAGAGATAG ACTTGGCTTC ATTGCGGGAA 
CCAGCTGGGA TATTCGAGCT GGTGGAGGTG GTTGGAAATG GAACCTATGG ACAAGTCTAC 
AAGGGGCGAC ATGTCAAGAC TGGACAACTG GCTGCCATCA AAGTTATGGA TGTCACAGAA 
GATGAGGAGG AGGAAATCAA ACTGGAGATC AATATGCTGA AGAAATATTC CCACCACAGA 
AACATAGCCA CCTATTATGG AGCTTTTATC AAGAAGAGCC CCCCGGGTCA CGATGACCAG 
CTGTGGCTGG TAATGGAGTT CTGTGGTGCC GGTTCGATCA CAGATCTGGT GAAGAACACG 
AAGGGAAACC AGCTGAAGGA AGACTGGATC GCTTACATCT CCAGAGAGAT CCTCAGAGGC 
CTTGCTCACC TACACGCCCA CCACGTGATC CACCGTGACA TTAAAGGCCA GAACGTTCTG 
CTGACAGAGA ACGCTGAAGT TAAACTGGTG GACTTCGGCG TGAGCGCCCA GCTGGATCGC 
ACAGTGGGGC GGAGAAACAC CTTCATCGGG ACTCCATATT GGATGGCGCC TGAGGTCATC 
GCTTGTGATG AGAACCCGGA TGCAACCTAT GATTACAGAA GCGATCTGTG GTCTTGCGGC 
ATCACAGCCA TCGAAATGGC CGAAGGAGCG CCGCCCCTTT GTGACATGCA CCCCATGCGT 
GCGCTCTTCC TCATTCCACG AAACCCCCCA CCGAGGCTCA AATCCAAGAA ATGGTCCAAG 
AAGTTTTTCA GCTTCATCGA GAGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCCCCACA 
GACCAGCTGC TGAAGCACCC GTTCATCCGA GACCAGCCCA ACGAGAGGCA GGTGCGCATC 
CAGCTCAAGG ACCACATCGA CCGGACCAAG AAGAAGAGGG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGATGAGGAG GAGGATCCTC CAGAGCAGGA AGGAGAACCG 
AGCTCCATCA TCAACGCAAC CGAGTCCACC CTGCGACGCG ACTTCATCCG CCTGCAGCAG 
GAGAACAAGG AGCGCTCCGA GGCCATGCGT CACCAGCAGC TCCTCCAGGA GCAGCAGATG 
CGGGAGCAAG AGGAGTACAA GCGGCAGCTC CTGGCAGAGA GGCAGAAGCG CATCGAGCAG 
CAGAAGGAGC AGAGGCGGCG GCTGGAGGAG CAACAACGAT GCGAACGGGA AATGAGGAGG 
CAGCAGGAGC GCGAGCAGCG GCGGCGCGAG CAGGAGGAGA AAAGACGAAT GGAGGAGATG 
GACCGCAGGC GAAAAGAGGA AGAGGAGCGC AGACGCGCCG AAGACGAAAA GAGAAGGAAC 
GATCGGGAGC AGGAGTACAT TAGACGGCAG CTGGAGGAGG AGCAGCGACA CCTGGAGAAG 
CTGCAGGAGC AGCTGCTGCG AGAGCAGGCC ATGCTGCTGG AGTTCAAGTG GCGGGAGCTG 
GACGAGCAGC GCAAAGCCGA GAGACTGCAT AAGCGGCTGC AGCTGGAGCA GGTGTACCTG 
CTGTCCCTGC AGCATGAATC CAAGCAGCAG CACCCTGCTG ACAGGACAGC CCCCTCCTCA 
GAGCCCAGCC ACACTCCCCA GGCTTTGACT CTGCCCCCGG CCTTCTTGCC GACCCCACGG 
GCTCAGGTCA TCGACGCTGT CGTTTCTCTG GCCTCTGAGC CCCCCAGAGT CCCCCAGATT 
ACCCCCTCAG ACAGGACAGT GCCTGAGAAG ACTGACGAGA CCAGTACCAG ACAGATTTCT 
CCCTCCCAAA CCCCCCAGCC CTCTGTAAGC GAAACCCCCA CCGACTCTAA GAGTCCCAAG 
GCAGAAAGTC TGGAGCCTGA CAGGATCTCC GAGCCTGTCA GGCTTCCCAT TCAGCCTATC 
AGAGAGGCGG ATGAGCGCTA CCGTAAAAAC ATCCAGGGCT CCCCGCAGGC CGCCCCTCCT 
CCCAAGCAGC CCCCTCTGCC ACCCCGATCC TCCGAACCCT TCTCCAACGG CGGCTCCTCC 
TCTGAGGCCA TGCACCGGCC CATGGAGCCT CAGGTCCAGT GGTCACACCT GGCCGCTCTT 
AAGAGCAGCA ACAGCGCCAC CCCCTCTCCT CCTCCGCCTG TTGTCGCTCG CTCCCAGTCC 
TTCAGCGAAT CCGGTGGCGT GGCGTCTAAC TTTGCACAAC TCCACCTGCG CTCCCAGGAC 
CCCCACCATC AACACCACCA CCACCCATCG CCTGCATTCA CTGACCCCCA CCCCCAACCT 
CCCCTCCACC ACCCCCAGGC TCAGAATAGG CTGGAACACC AGTCCAGCAG TGAAGAGGTT 
CCTCCAAAGG TACCAGTGAG GACAACATCC AGATCTCCGG TTTTGTCGCG GCGAGAGTCT 
CCTCTGCCAA CGCAGCCCAA CAACCAGAGG AACGCCGTCG GGAACATGGA GCAGCGCCCT 
CTATGGGACA GGGTGGAGAA GCTGCAGTCC CGACCCGGCA GCGGCAGCTC CTCCAACTCC 
AACTCTCAGG CCAGTCCCGG CGAGCGTTTC AGACCTCGCT GTGAGTCCCC TGCTTCCTCT 
AAATCTGAGG GATCGCCTCT CCAGCGGCCC GAAAACCTCC CCAAAAAACA GGACGAAAGG 
AACGTGGCCC GGCCGACGCG ACCCGCTGAC CTGACGGCGC TGGCTAAGGA GCTGCGTGCC 
GTGGACGACG TGCGGCCCCC CCACAAGGTC ACAGACTACT CCTCATCGAG CGATGACTCG 
GGCACCACGG ATGAGGAAGA CGACGAGGAG GTGGACCAGG AGCCAGGGGA CGAGTCCACG 
TCGGGGGCCG AGGACTCCAG AGCTGGGAGG CTCAGAAACG GGGAGGCAGA CTCTGCTAAG 
ACCATGCTGG TGGAGGACTC TGAGAGTGAG CAGGGCACCA CCCCCTCCAA AGACGGGACC 
CTGGTCATCA GACAGAGCAC AGCTGACATA AAGCGGTTGG TCAATGTCTC GTCTTCATCA 
TCCTCCTCCT CGCCCGGCTC CGGTCGTGGC CCCCCAGAGA AAAACGGCCG CATGTACCAC 
CTCCCCGACA TTATTCAGCA GAGCCATCAT TCCTCCCCCC CCTCCACAGC CAGCTTCTCC 
TCCTTGTCCT CCTCCTCCTC CTCCTCCTTC CCCTCATCAT CTAGCCAGGC CAGTCTTGGC 
GTCTCCTCAC AGATCTCCTT GGAGGAGCTC GTAGCCGTAG AGTCGCAGTC AGAGAGCAAC 
TCCATGTCCA AACACAAGTC TTCCTCTTCG TTCACTCCCT TCATCGACCC ACGCCTCCTC 
CAGATCTCTC CATCAGGCGG CAGCTCCCTC AACAACATGG CGGTTTTTGG ACAAGAAGGC 
CGACCCGCCG ACCCACTGCG CTCTGACCCC TCCCGTAAAG GCTCGGTGGT CAACGTCAAC 
CCTGTCAACA CTCGTCCGCC CAGCGACACT CCCGAGATCC GCAAGTACAA GAAGAGGTTC 
AACTCCGAGA TTCTGTGCGC GGCGCTCTGG GGGGTGAACC TGCTGGTGGG GACAGAAAGC 
GGGCTGATGC TGCTGGACCG GAGCGGTCAG GGTAAGGTTT ACCCGCTGAT TAATCGACGC 
CGCATCCAGC AGATGGATGT CCTGGAGGGA CTCAACGTCC TGGTAACCAT ATCAGGTAAA 
AAGAACAAAC TGCGAGTGTA TTACCTGTCG TGGCTCCGAA ACAAGATTTT GCACAACGAC 
CCAGAAGTGG AGAAGAAGCA GGGCTGGGTC AACGTGGGCG ACCTGGAGGG CTGCGTTCAC 
TACAAAGTCG TAAAGTACGA GAGGATCAAG TTCCTGGTTC TGGCTTTGAA GAACGCTGTG 
GAGGTGTACG CCTGGGCGCC CAAACCCTAC CACAAGTTCA TGGCCTTCAA GTCGTTTGGT 
GACCTGGTGC ACAAACCTCT GCTGGTGGAC CTGACTGTGG AGGAAGGCCA AAGGTTAAAG 
GTCATCTACG GATCCTGCTC GGGCTTCCAC GCTGTGGATG TGGACTCGGG CGCCGTCTAC 
GACATCTACC TCCCCACACA TATCCAGACC AGCATCCAGT GCCACGCCAT CATCATCCTA 
CCCAACACTG ACGGCATCGA GCTGCTGGTC TGTTACGAGG ACGAGGGCGT CTACGTCAAC 
ACGTACGGGC GCATCACCAA AGACGTGGTG CTGCAGTGGG GAGAAATGCC GACTTCCGTG 
GCCTACATTA GGTCAAACCA GATCATGGGC TGGGGCGAAA AGGCCATCGA GATCCGCTCG 
GTGGAGACGG GACACCTGGA CGGCGTCTTC ATGCACAAGC GAGCCCAGAG ACTCAAATTC 
CTCTGTGAAA GAAACGACAA GGTCTTTTTC GCCTCCGTGC GAGCCGGAGG CGCCAGTCAA 
GTATACTTCA TGACGCTGGG ACGCTCTTCC CTCATGAGCT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023821699.1
CDS	221..4645
Translation	MANDSPAKSLVEIDLASLREPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNQLKEDWIAYISREILRGLAHLHAHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIESCLVKNYMQRPPTDQLLKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEDEEEDPPEQEGEPSSIINATESTLRRDFIRLQQENKERSEAMRHQQLLQEQQMREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRCEREMRRQQEREQRRREQEEKRRMEEMDRRRKEEEERRRAEDEKRRNDREQEYIRRQLEEEQRHLEKLQEQLLREQAMLLEFKWRELDEQRKAERLHKRLQLEQVYLLSLQHESKQQHPADRTAPSSEPSHTPQALTLPPAFLPTPRAQVIDAVVSLASEPPRVPQITPSDRTVPEKTDETSTRQISPSQTPQPSVSETPTDSKSPKAESLEPDRISEPVRLPIQPIREADERYRKNIQGSPQAAPPPKQPPLPPRSSEPFSNGGSSSEAMHRPMEPQVQWSHLAALKSSNSATPSPPPPVVARSQSFSESGGVASNFAQLHLRSQDPHHQHHHHPSPAFTDPHPQPPLHHPQAQNRLEHQSSSEEVPPKVPVRTTSRSPVLSRRESPLPTQPNNQRNAVGNMEQRPLWDRVEKLQSRPGSGSSSNSNSQASPGERFRPRCESPASSKSEGSPLQRPENLPKKQDERNVARPTRPADLTALAKELRAVDDVRPPHKVTDYSSSSDDSGTTDEEDDEEVDQEPGDESTSGAEDSRAGRLRNGEADSAKTMLVEDSESEQGTTPSKDGTLVIRQSTADIKRLVNVSSSSSSSSPGSGRGPPEKNGRMYHLPDIIQQSHHSSPPSTASFSSLSSSSSSSFPSSSSQASLGVSSQISLEELVAVESQSESNSMSKHKSSSSFTPFIDPRLLQISPSGGSSLNNMAVFGQEGRPADPLRSDPSRKGSVVNVNPVNTRPPSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRIQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNKILHNDPEVEKKQGWVNVGDLEGCVHYKVVKYERIKFLVLALKNAVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCSGFHAVDVDSGAVYDIYLPTHIQTSIQCHAIIILPNTDGIELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRAGGASQVYFMTLGRSSLMSW

Target ORF information:

RefSeq Version	XM_023965931.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023965931.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCAGC TAAAAGTCTG GTAGAGATAG ACTTGGCTTC ATTGCGGGAA 
CCAGCTGGGA TATTCGAGCT GGTGGAGGTG GTTGGAAATG GAACCTATGG ACAAGTCTAC 
AAGGGGCGAC ATGTCAAGAC TGGACAACTG GCTGCCATCA AAGTTATGGA TGTCACAGAA 
GATGAGGAGG AGGAAATCAA ACTGGAGATC AATATGCTGA AGAAATATTC CCACCACAGA 
AACATAGCCA CCTATTATGG AGCTTTTATC AAGAAGAGCC CCCCGGGTCA CGATGACCAG 
CTGTGGCTGG TAATGGAGTT CTGTGGTGCC GGTTCGATCA CAGATCTGGT GAAGAACACG 
AAGGGAAACC AGCTGAAGGA AGACTGGATC GCTTACATCT CCAGAGAGAT CCTCAGAGGC 
CTTGCTCACC TACACGCCCA CCACGTGATC CACCGTGACA TTAAAGGCCA GAACGTTCTG 
CTGACAGAGA ACGCTGAAGT TAAACTGGTG GACTTCGGCG TGAGCGCCCA GCTGGATCGC 
ACAGTGGGGC GGAGAAACAC CTTCATCGGG ACTCCATATT GGATGGCGCC TGAGGTCATC 
GCTTGTGATG AGAACCCGGA TGCAACCTAT GATTACAGAA GCGATCTGTG GTCTTGCGGC 
ATCACAGCCA TCGAAATGGC CGAAGGAGCG CCGCCCCTTT GTGACATGCA CCCCATGCGT 
GCGCTCTTCC TCATTCCACG AAACCCCCCA CCGAGGCTCA AATCCAAGAA ATGGTCCAAG 
AAGTTTTTCA GCTTCATCGA GAGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCCCCACA 
GACCAGCTGC TGAAGCACCC GTTCATCCGA GACCAGCCCA ACGAGAGGCA GGTGCGCATC 
CAGCTCAAGG ACCACATCGA CCGGACCAAG AAGAAGAGGG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGATGAGGAG GAGGATCCTC CAGAGCAGGA AGGAGAACCG 
AGCTCCATCA TCAACGCAAC CGAGTCCACC CTGCGACGCG ACTTCATCCG CCTGCAGCAG 
GAGAACAAGG AGCGCTCCGA GGCCATGCGT CACCAGCAGC TCCTCCAGGA GCAGCAGATG 
CGGGAGCAAG AGGAGTACAA GCGGCAGCTC CTGGCAGAGA GGCAGAAGCG CATCGAGCAG 
CAGAAGGAGC AGAGGCGGCG GCTGGAGGAG CAACAACGAT GCGAACGGGA AATGAGGAGG 
CAGCAGGAGC GCGAGCAGCG GCGGCGCGAG CAGGAGGAGA AAAGACGAAT GGAGGAGATG 
GACCGCAGGC GAAAAGAGGA AGAGGAGCGC AGACGCGCCG AAGACGAAAA GAGAAGGAAC 
GATCGGGAGC AGGAGTACAT TAGACGGCAG CTGGAGGAGG AGCAGCGACA CCTGGAGAAG 
CTGCAGGAGC AGCTGCTGCG AGAGCAGGCC ATGCTGCTGG AGTTCAAGTG GCGGGAGCTG 
GACGAGCAGC GCAAAGCCGA GAGACTGCAT AAGCGGCTGC AGCTGGAGCA GGTGTACCTG 
CTGTCCCTGC AGCATGAATC CAAGCAGCAG CACCCTGCTG ACAGGACAGC CCCCTCCTCA 
GAGCCCAGCC ACACTCCCCA GGCTTTGACT CTGCCCCCGG CCTTCTTGCC GACCCCACGG 
GCTCAGGTCA TCGACGCTGT CGTTTCTCTG GCCTCTGAGC CCCCCAGAGT CCCCCAGATT 
ACCCCCTCAG ACAGGACAGT GCCTGAGAAG ACTGACGAGA CCAGTACCAG ACAGATTTCT 
CCCTCCCAAA CCCCCCAGCC CTCTGTAAGC GAAACCCCCA CCGACTCTAA GAGTCCCAAG 
GCAGAAAGTC TGGAGCCTGA CAGGATCTCC GAGCCTGTCA GGCTTCCCAT TCAGCCTATC 
AGAGAGGCGG ATGAGCGCTA CCGTAAAAAC ATCCAGGGCT CCCCGCAGGC CGCCCCTCCT 
CCCAAGCAGC CCCCTCTGCC ACCCCGATCC TCCGAACCCT TCTCCAACGG CGGCTCCTCC 
TCTGAGGCCA TGCACCGGCC CATGGAGCCT CAGGTCCAGT GGTCACACCT GGCCGCTCTT 
AAGAGCAGCA ACAGCGCCAC CCCCTCTCCT CCTCCGCCTG TTGTCGCTCG CTCCCAGTCC 
TTCAGCGAAT CCGGTGGCGT GGCGTCTAAC TTTGCACAAC TCCACCTGCG CTCCCAGGAC 
CCCCACCATC AACACCACCA CCACCCATCG CCTGCATTCA CTGACCCCCA CCCCCAACCT 
CCCCTCCACC ACCCCCAGGC TCAGAATAGG CTGGAACACC AGTCCAGCAG TGAAGAGGTT 
CCTCCAAAGG TACCAGTGAG GACAACATCC AGATCTCCGG TTTTGTCGCG GCGAGAGTCT 
CCTCTGCCAA CGCAGCCCAA CAACCAGAGG AACGCCGTCG GGAACATGGA GCAGCGCCCT 
CTATGGGACA GGGTGGAGAA GCTGCAGTCC CGACCCGGCA GCGGCAGCTC CTCCAACTCC 
AACTCTCAGG CCAGTCCCGG CGAGCGTTTC AGACCTCGCT GTGAGTCCCC TGCTTCCTCT 
AAATCTGAGG GATCGCCTCT CCAGCGGCCC GAAAACCTCC CCAAAAAACA GGACGAAAGG 
AACGTGGCCC GGCCGACGCG ACCCGCTGAC CTGACGGCGC TGGCTAAGGA GCTGCGTGCC 
GTGGACGACG TGCGGCCCCC CCACAAGGTC ACAGACTACT CCTCATCGAG CGATGACTCG 
GGCACCACGG ATGAGGAAGA CGACGAGGAG GTGGACCAGG AGCCAGGGGA CGAGTCCACG 
TCGGGGGCCG AGGACTCCAG AGCTGGGAGG CTCAGAAACG GGGAGGCAGA CTCTGCTAAG 
ACCATGCTGG TGGAGGACTC TGAGAGTGAG CAGGGCACCA CCCCCTCCAA AGACGGGACC 
CTGGTCATCA GACAGAGCAC AGCTGACATA AAGCGGTTGG TCAATGTCTC GTCTTCATCA 
TCCTCCTCCT CGCCCGGCTC CGGTCGTGGC CCCCCAGAGA AAAACGGCCG CATGTACCAC 
CTCCCCGACA TTATTCAGCA GAGCCATCAT TCCTCCCCCC CCTCCACAGC CAGCTTCTCC 
TCCTTGTCCT CCTCCTCCTC CTCCTCCTTC CCCTCATCAT CTAGCCAGGC CAGTCTTGGC 
GTCTCCTCAC AGATCTCCTT GGAGGAGCTC GTAGCCGTAG AGTCGCAGTC AGAGAGCAAC 
TCCATGTCCA AACACAAGTC TTCCTCTTCG TTCACTCCCT TCATCGACCC ACGCCTCCTC 
CAGATCTCTC CATCAGGCGG CAGCTCCCTC AACAACATGG CGGTTTTTGG ACAAGAAGGC 
CGACCCGCCG ACCCACTGCG CTCTGACCCC TCCCGTAAAG GCTCGGTGGT CAACGTCAAC 
CCTGTCAACA CTCGTCCGCC CAGCGACACT CCCGAGATCC GCAAGTACAA GAAGAGGTTC 
AACTCCGAGA TTCTGTGCGC GGCGCTCTGG GGGGTGAACC TGCTGGTGGG GACAGAAAGC 
GGGCTGATGC TGCTGGACCG GAGCGGTCAG GGTAAGGTTT ACCCGCTGAT TAATCGACGC 
CGCATCCAGC AGATGGATGT CCTGGAGGGA CTCAACGTCC TGGTAACCAT ATCAGGTAAA 
AAGAACAAAC TGCGAGTGTA TTACCTGTCG TGGCTCCGAA ACAAGATTTT GCACAACGAC 
CCAGAAGTGG AGAAGAAGCA GGGCTGGGTC AACGTGGGCG ACCTGGAGGG CTGCGTTCAC 
TACAAAGTCG TAAAGTACGA GAGGATCAAG TTCCTGGTTC TGGCTTTGAA GAACGCTGTG 
GAGGTGTACG CCTGGGCGCC CAAACCCTAC CACAAGTTCA TGGCCTTCAA GTCGTTTGGT 
GACCTGGTGC ACAAACCTCT GCTGGTGGAC CTGACTGTGG AGGAAGGCCA AAGGTTAAAG 
GTCATCTACG GATCCTGCTC GGGCTTCCAC GCTGTGGATG TGGACTCGGG CGCCGTCTAC 
GACATCTACC TCCCCACACA TATCCAGACC AGCATCCAGT GCCACGCCAT CATCATCCTA 
CCCAACACTG ACGGCATCGA GCTGCTGGTC TGTTACGAGG ACGAGGGCGT CTACGTCAAC 
ACGTACGGGC GCATCACCAA AGACGTGGTG CTGCAGTGGG GAGAAATGCC GACTTCCGTG 
GCCTACATTA GGTCAAACCA GATCATGGGC TGGGGCGAAA AGGCCATCGA GATCCGCTCG 
GTGGAGACGG GACACCTGGA CGGCGTCTTC ATGCACAAGC GAGCCCAGAG ACTCAAATTC 
CTCTGTGAAA GAAACGACAA GGTCTTTTTC GCCTCCGTGC GAGCCGGAGG CGCCAGTCAA 
GTATACTTCA TGACGCTGGG ACGCTCTTCC CTCATGAGCT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101174648 ( XM_023965937.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023821705.1 Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OJa112414
Clone ID Related Accession (Same CDS sequence)	XM_023965937.1
Accession Version	XM_023965937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCAGC TAAAAGTCTG GTAGAGATAG ACTTGGCTTC ATTGCGGGAA 
CCAGCTGGGA TATTCGAGCT GGTGGAGGTG GTTGGAAATG GAACCTATGG ACAAGTCTAC 
AAGGGGCGAC ATGTCAAGAC TGGACAACTG GCTGCCATCA AAGTTATGGA TGTCACAGAA 
GATGAGGAGG AGGAAATCAA ACTGGAGATC AATATGCTGA AGAAATATTC CCACCACAGA 
AACATAGCCA CCTATTATGG AGCTTTTATC AAGAAGAGCC CCCCGGGTCA CGATGACCAG 
CTGTGGCTGG TAATGGAGTT CTGTGGTGCC GGTTCGATCA CAGATCTGGT GAAGAACACG 
AAGGGAAACC AGCTGAAGGA AGACTGGATC GCTTACATCT CCAGAGAGAT CCTCAGAGGC 
CTTGCTCACC TACACGCCCA CCACGTGATC CACCGTGACA TTAAAGGCCA GAACGTTCTG 
CTGACAGAGA ACGCTGAAGT TAAACTGGTG GACTTCGGCG TGAGCGCCCA GCTGGATCGC 
ACAGTGGGGC GGAGAAACAC CTTCATCGGG ACTCCATATT GGATGGCGCC TGAGGTCATC 
GCTTGTGATG AGAACCCGGA TGCAACCTAT GATTACAGAA GCGATCTGTG GTCTTGCGGC 
ATCACAGCCA TCGAAATGGC CGAAGGAGCG CCGCCCCTTT GTGACATGCA CCCCATGCGT 
GCGCTCTTCC TCATTCCACG AAACCCCCCA CCGAGGCTCA AATCCAAGAA ATGGTCCAAG 
AAGTTTTTCA GCTTCATCGA GAGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCCCCACA 
GACCAGCTGC TGAAGCACCC GTTCATCCGA GACCAGCCCA ACGAGAGGCA GGTGCGCATC 
CAGCTCAAGG ACCACATCGA CCGGACCAAG AAGAAGAGGG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGATGAGGAG GAGGATCCTC CAGAGCAGGA AGGAGAACCG 
AGCTCCATCA TCAACGCAAC CGAGTCCACC CTGCGACGCG ACTTCATCCG CCTGCAGCAG 
GAGAACAAGG AGCGCTCCGA GGCCATGCGT CACCAGCAGC TCCTCCAGGA GCAGCAGATG 
CGGGAGCAAG AGGAGTACAA GCGGCAGCTC CTGGCAGAGA GGCAGAAGCG CATCGAGCAG 
CAGAAGGAGC AGAGGCGGCG GCTGGAGGAG CAACAACGAT GCGAACGGGA AATGAGGAGG 
CAGCAGGAGC GCGAGCAGCG GCGGCGCGAG CAGGAGGAGA AAAGACGAAT GGAGGAGATG 
GACCGCAGGC GAAAAGAGGA AGAGGAGCGC AGACGCGCCG AAGACGAAAA GAGAAGGAAC 
GATCGGGAGC AGGAGTACAT TAGACGGCAG CTGGAGGAGG AGCAGCGACA CCTGGAGAAG 
CTGCAGGAGC AGCTGCTGCG AGAGCAGGCC ATGCTGCTGG AGTTCAAGTG GCGGGAGCTG 
GACGAGCAGC GCAAAGCCGA GAGACTGCAT AAGCGGCTGC AGCTGGAGCA GGTGTACCTG 
CTGTCCCTGC AGCATGAATC CAAGCAGCAG CACCCTGCTG ACAGGACAGC CCCCTCCTCA 
GAGCCCAGCC ACACTCCCCA GGCTTTGACT CTGCCCCCGG CCTTCTTGCC GACCCCACGG 
GCTCAGGTCA TCGACGCTGT CGTTTCTCTG GCCTCTGAGC CCCCCAGAGT CCCCCAGATT 
ACCCCCTCAG ACAGGACAGT GCCTGAGAAG ACTGACGAGA CCAGTACCAG ACAGATTTCT 
CCCTCCCAAA CCCCCCAGCC CTCTGTAAGC GAAACCCCCA CCGACTCTAA GAGTCCCAAG 
GCAGAAAGTC TGGAGCCTGA CAGGATCTCC GAGCCTGTCA GGCTTCCCAT TCAGCCTATC 
AGAGAGGCGG ATGAGCGCTA CCGTAAAAAC ATCCAGGGCT CCCCGCAGGC CGCCCCTCCT 
CCCAAGCAGC CCCCTCTGCC ACCCCGATCC TCCGAACCCT TCTCCAACGG CGGCTCCTCC 
TCTGAGGCCA TGCACCGGCC CATGGAGCCT CAGGTCCAGT GGTCACACCT GGCCGCTCTT 
AAGAGCAGCA ACAGCGCCAC CCCCTCTCCT CCTCCGCCTG TTGTCGCTCG CTCCCAGTCC 
TTCAGCGAAT CCGGTGGCGT GGCGTCTAAC TTTGCACAAC TCCACCTGCG CTCCCAGGAC 
CCCCACCATC AACACCACCA CCACCCATCG CCTGCATTCA CTGACCCCCA CCCCCAACCT 
CCCCTCCACC ACCCCCAGGC TCAGAATAGG CTGGAACACC AGTCCAGCAG TGAAGAGGTT 
CCTCCAAAGG TACCAGTGAG GACAACATCC AGATCTCCGG TTTTGTCGCG GCGAGAGTCT 
CCTCTGCCAA CGCAGCCCAA CAACCAGAGG AACGCCGTCG GGAACATGGA GCAGCGCCCT 
CTATGGGACA GGGTGGAGAA GCTGCAGTCC CGACCCGGCA GCGGCAGCTC CTCCAACTCC 
AACTCTCAGG CCAGTCCCGG CGAGCGTTTC AGACCTCGCT CTTCCTCTAA ATCTGAGGGA 
TCGCCTCTCC AGCGGCCCGA AAACCTCCCC AAAAAACAGG ACGAAAGGAA CGTGGCCCGG 
CCGACGCGAC CCGCTGACCT GACGGCGCTG GCTAAGGAGC TGCGTGCCGT GGACGACGTG 
CGGCCCCCCC ACAAGGTCAC AGACTACTCC TCATCGAGCG ATGACTCGGG CACCACGGAT 
GAGGAAGACG ACGAGGAGGT GGACCAGGAG CCAGGGGACG AGTCCACGTC GGGGGCCGAG 
GACTCCAGAG CTGGGAGGCT CAGAAACGGG GAGGCAGACT CTGCTAAGAC CATGCTGGTG 
GAGGACTCTG AGAGTGAGCA GGGCACCACC CCCTCCAAAG ACGGGACCCT GGTCATCAGA 
CAGAGCACAG CTGACATAAA GCGGTTGGTC AATGTCTCGT CTTCATCATC CTCCTCCTCG 
CCCGGCTCCG GTCGTGGCCC CCCAGAGAAA AACGGCCGCA TGTACCACCT CCCCGACATT 
ATTCAGCAGA GCCATCATTC CTCCCCCCCC TCCACAGCCA GCTTCTCCTC CTTGTCCTCC 
TCCTCCTCCT CCTCCTTCCC CTCATCATCT AGCCAGGCCA GTCTTGGCGT CTCCTCACAG 
ATCTCCTTGG AGGAGCTCGT AGCCGTAGAG TCGCAGTCAG AGAGCAACTC CATGTCCAAA 
CACAAGTCTT CCTCTTCGTT CACTCCCTTC ATCGACCCAC GCCTCCTCCA GATCTCTCCA 
TCAGGCGGCA GCTCCCTCAA CAACATGGCG GTTTTTGGAC AAGAAGGCCG ACCCGCCGAC 
CCACTGCGCT CTGACCCCTC CCGTAAAGGC TCGGTGGTCA ACGTCAACCC TGTCAACACT 
CGTCCGCCCA GCGACACTCC CGAGATCCGC AAGTACAAGA AGAGGTTCAA CTCCGAGATT 
CTGTGCGCGG CGCTCTGGGG GGTGAACCTG CTGGTGGGGA CAGAAAGCGG GCTGATGCTG 
CTGGACCGGA GCGGTCAGGG TAAGGTTTAC CCGCTGATTA ATCGACGCCG CATCCAGCAG 
ATGGATGTCC TGGAGGGACT CAACGTCCTG GTAACCATAT CAGGTAAAAA GAACAAACTG 
CGAGTGTATT ACCTGTCGTG GCTCCGAAAC AAGATTTTGC ACAACGACCC AGAAGTGGAG 
AAGAAGCAGG GCTGGGTCAA CGTGGGCGAC CTGGAGGGCT GCGTTCACTA CAAAGTCGTA 
AAGTACGAGA GGATCAAGTT CCTGGTTCTG GCTTTGAAGA ACGCTGTGGA GGTGTACGCC 
TGGGCGCCCA AACCCTACCA CAAGTTCATG GCCTTCAAGT CGTTTGGTGA CCTGGTGCAC 
AAACCTCTGC TGGTGGACCT GACTGTGGAG GAAGGCCAAA GGTTAAAGGT CATCTACGGA 
TCCTGCTCGG GCTTCCACGC TGTGGATGTG GACTCGGGCG CCGTCTACGA CATCTACCTC 
CCCACACATA TCCAGACCAG CATCCAGTGC CACGCCATCA TCATCCTACC CAACACTGAC 
GGCATCGAGC TGCTGGTCTG TTACGAGGAC GAGGGCGTCT ACGTCAACAC GTACGGGCGC 
ATCACCAAAG ACGTGGTGCT GCAGTGGGGA GAAATGCCGA CTTCCGTGGC CTACATTAGG 
TCAAACCAGA TCATGGGCTG GGGCGAAAAG GCCATCGAGA TCCGCTCGGT GGAGACGGGA 
CACCTGGACG GCGTCTTCAT GCACAAGCGA GCCCAGAGAC TCAAATTCCT CTGTGAAAGA 
AACGACAAGG TCTTTTTCGC CTCCGTGCGA GCCGGAGGCG CCAGTCAAGT ATACTTCATG 
ACGCTGGGAC GCTCTTCCCT CATGAGCTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023821705.1
CDS	221..4633
Translation	MANDSPAKSLVEIDLASLREPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNQLKEDWIAYISREILRGLAHLHAHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIESCLVKNYMQRPPTDQLLKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEDEEEDPPEQEGEPSSIINATESTLRRDFIRLQQENKERSEAMRHQQLLQEQQMREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRCEREMRRQQEREQRRREQEEKRRMEEMDRRRKEEEERRRAEDEKRRNDREQEYIRRQLEEEQRHLEKLQEQLLREQAMLLEFKWRELDEQRKAERLHKRLQLEQVYLLSLQHESKQQHPADRTAPSSEPSHTPQALTLPPAFLPTPRAQVIDAVVSLASEPPRVPQITPSDRTVPEKTDETSTRQISPSQTPQPSVSETPTDSKSPKAESLEPDRISEPVRLPIQPIREADERYRKNIQGSPQAAPPPKQPPLPPRSSEPFSNGGSSSEAMHRPMEPQVQWSHLAALKSSNSATPSPPPPVVARSQSFSESGGVASNFAQLHLRSQDPHHQHHHHPSPAFTDPHPQPPLHHPQAQNRLEHQSSSEEVPPKVPVRTTSRSPVLSRRESPLPTQPNNQRNAVGNMEQRPLWDRVEKLQSRPGSGSSSNSNSQASPGERFRPRSSSKSEGSPLQRPENLPKKQDERNVARPTRPADLTALAKELRAVDDVRPPHKVTDYSSSSDDSGTTDEEDDEEVDQEPGDESTSGAEDSRAGRLRNGEADSAKTMLVEDSESEQGTTPSKDGTLVIRQSTADIKRLVNVSSSSSSSSPGSGRGPPEKNGRMYHLPDIIQQSHHSSPPSTASFSSLSSSSSSSFPSSSSQASLGVSSQISLEELVAVESQSESNSMSKHKSSSSFTPFIDPRLLQISPSGGSSLNNMAVFGQEGRPADPLRSDPSRKGSVVNVNPVNTRPPSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRIQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNKILHNDPEVEKKQGWVNVGDLEGCVHYKVVKYERIKFLVLALKNAVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCSGFHAVDVDSGAVYDIYLPTHIQTSIQCHAIIILPNTDGIELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRAGGASQVYFMTLGRSSLMSW

Target ORF information:

RefSeq Version	XM_023965937.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023965937.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCAGC TAAAAGTCTG GTAGAGATAG ACTTGGCTTC ATTGCGGGAA 
CCAGCTGGGA TATTCGAGCT GGTGGAGGTG GTTGGAAATG GAACCTATGG ACAAGTCTAC 
AAGGGGCGAC ATGTCAAGAC TGGACAACTG GCTGCCATCA AAGTTATGGA TGTCACAGAA 
GATGAGGAGG AGGAAATCAA ACTGGAGATC AATATGCTGA AGAAATATTC CCACCACAGA 
AACATAGCCA CCTATTATGG AGCTTTTATC AAGAAGAGCC CCCCGGGTCA CGATGACCAG 
CTGTGGCTGG TAATGGAGTT CTGTGGTGCC GGTTCGATCA CAGATCTGGT GAAGAACACG 
AAGGGAAACC AGCTGAAGGA AGACTGGATC GCTTACATCT CCAGAGAGAT CCTCAGAGGC 
CTTGCTCACC TACACGCCCA CCACGTGATC CACCGTGACA TTAAAGGCCA GAACGTTCTG 
CTGACAGAGA ACGCTGAAGT TAAACTGGTG GACTTCGGCG TGAGCGCCCA GCTGGATCGC 
ACAGTGGGGC GGAGAAACAC CTTCATCGGG ACTCCATATT GGATGGCGCC TGAGGTCATC 
GCTTGTGATG AGAACCCGGA TGCAACCTAT GATTACAGAA GCGATCTGTG GTCTTGCGGC 
ATCACAGCCA TCGAAATGGC CGAAGGAGCG CCGCCCCTTT GTGACATGCA CCCCATGCGT 
GCGCTCTTCC TCATTCCACG AAACCCCCCA CCGAGGCTCA AATCCAAGAA ATGGTCCAAG 
AAGTTTTTCA GCTTCATCGA GAGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCCCCACA 
GACCAGCTGC TGAAGCACCC GTTCATCCGA GACCAGCCCA ACGAGAGGCA GGTGCGCATC 
CAGCTCAAGG ACCACATCGA CCGGACCAAG AAGAAGAGGG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGATGAGGAG GAGGATCCTC CAGAGCAGGA AGGAGAACCG 
AGCTCCATCA TCAACGCAAC CGAGTCCACC CTGCGACGCG ACTTCATCCG CCTGCAGCAG 
GAGAACAAGG AGCGCTCCGA GGCCATGCGT CACCAGCAGC TCCTCCAGGA GCAGCAGATG 
CGGGAGCAAG AGGAGTACAA GCGGCAGCTC CTGGCAGAGA GGCAGAAGCG CATCGAGCAG 
CAGAAGGAGC AGAGGCGGCG GCTGGAGGAG CAACAACGAT GCGAACGGGA AATGAGGAGG 
CAGCAGGAGC GCGAGCAGCG GCGGCGCGAG CAGGAGGAGA AAAGACGAAT GGAGGAGATG 
GACCGCAGGC GAAAAGAGGA AGAGGAGCGC AGACGCGCCG AAGACGAAAA GAGAAGGAAC 
GATCGGGAGC AGGAGTACAT TAGACGGCAG CTGGAGGAGG AGCAGCGACA CCTGGAGAAG 
CTGCAGGAGC AGCTGCTGCG AGAGCAGGCC ATGCTGCTGG AGTTCAAGTG GCGGGAGCTG 
GACGAGCAGC GCAAAGCCGA GAGACTGCAT AAGCGGCTGC AGCTGGAGCA GGTGTACCTG 
CTGTCCCTGC AGCATGAATC CAAGCAGCAG CACCCTGCTG ACAGGACAGC CCCCTCCTCA 
GAGCCCAGCC ACACTCCCCA GGCTTTGACT CTGCCCCCGG CCTTCTTGCC GACCCCACGG 
GCTCAGGTCA TCGACGCTGT CGTTTCTCTG GCCTCTGAGC CCCCCAGAGT CCCCCAGATT 
ACCCCCTCAG ACAGGACAGT GCCTGAGAAG ACTGACGAGA CCAGTACCAG ACAGATTTCT 
CCCTCCCAAA CCCCCCAGCC CTCTGTAAGC GAAACCCCCA CCGACTCTAA GAGTCCCAAG 
GCAGAAAGTC TGGAGCCTGA CAGGATCTCC GAGCCTGTCA GGCTTCCCAT TCAGCCTATC 
AGAGAGGCGG ATGAGCGCTA CCGTAAAAAC ATCCAGGGCT CCCCGCAGGC CGCCCCTCCT 
CCCAAGCAGC CCCCTCTGCC ACCCCGATCC TCCGAACCCT TCTCCAACGG CGGCTCCTCC 
TCTGAGGCCA TGCACCGGCC CATGGAGCCT CAGGTCCAGT GGTCACACCT GGCCGCTCTT 
AAGAGCAGCA ACAGCGCCAC CCCCTCTCCT CCTCCGCCTG TTGTCGCTCG CTCCCAGTCC 
TTCAGCGAAT CCGGTGGCGT GGCGTCTAAC TTTGCACAAC TCCACCTGCG CTCCCAGGAC 
CCCCACCATC AACACCACCA CCACCCATCG CCTGCATTCA CTGACCCCCA CCCCCAACCT 
CCCCTCCACC ACCCCCAGGC TCAGAATAGG CTGGAACACC AGTCCAGCAG TGAAGAGGTT 
CCTCCAAAGG TACCAGTGAG GACAACATCC AGATCTCCGG TTTTGTCGCG GCGAGAGTCT 
CCTCTGCCAA CGCAGCCCAA CAACCAGAGG AACGCCGTCG GGAACATGGA GCAGCGCCCT 
CTATGGGACA GGGTGGAGAA GCTGCAGTCC CGACCCGGCA GCGGCAGCTC CTCCAACTCC 
AACTCTCAGG CCAGTCCCGG CGAGCGTTTC AGACCTCGCT CTTCCTCTAA ATCTGAGGGA 
TCGCCTCTCC AGCGGCCCGA AAACCTCCCC AAAAAACAGG ACGAAAGGAA CGTGGCCCGG 
CCGACGCGAC CCGCTGACCT GACGGCGCTG GCTAAGGAGC TGCGTGCCGT GGACGACGTG 
CGGCCCCCCC ACAAGGTCAC AGACTACTCC TCATCGAGCG ATGACTCGGG CACCACGGAT 
GAGGAAGACG ACGAGGAGGT GGACCAGGAG CCAGGGGACG AGTCCACGTC GGGGGCCGAG 
GACTCCAGAG CTGGGAGGCT CAGAAACGGG GAGGCAGACT CTGCTAAGAC CATGCTGGTG 
GAGGACTCTG AGAGTGAGCA GGGCACCACC CCCTCCAAAG ACGGGACCCT GGTCATCAGA 
CAGAGCACAG CTGACATAAA GCGGTTGGTC AATGTCTCGT CTTCATCATC CTCCTCCTCG 
CCCGGCTCCG GTCGTGGCCC CCCAGAGAAA AACGGCCGCA TGTACCACCT CCCCGACATT 
ATTCAGCAGA GCCATCATTC CTCCCCCCCC TCCACAGCCA GCTTCTCCTC CTTGTCCTCC 
TCCTCCTCCT CCTCCTTCCC CTCATCATCT AGCCAGGCCA GTCTTGGCGT CTCCTCACAG 
ATCTCCTTGG AGGAGCTCGT AGCCGTAGAG TCGCAGTCAG AGAGCAACTC CATGTCCAAA 
CACAAGTCTT CCTCTTCGTT CACTCCCTTC ATCGACCCAC GCCTCCTCCA GATCTCTCCA 
TCAGGCGGCA GCTCCCTCAA CAACATGGCG GTTTTTGGAC AAGAAGGCCG ACCCGCCGAC 
CCACTGCGCT CTGACCCCTC CCGTAAAGGC TCGGTGGTCA ACGTCAACCC TGTCAACACT 
CGTCCGCCCA GCGACACTCC CGAGATCCGC AAGTACAAGA AGAGGTTCAA CTCCGAGATT 
CTGTGCGCGG CGCTCTGGGG GGTGAACCTG CTGGTGGGGA CAGAAAGCGG GCTGATGCTG 
CTGGACCGGA GCGGTCAGGG TAAGGTTTAC CCGCTGATTA ATCGACGCCG CATCCAGCAG 
ATGGATGTCC TGGAGGGACT CAACGTCCTG GTAACCATAT CAGGTAAAAA GAACAAACTG 
CGAGTGTATT ACCTGTCGTG GCTCCGAAAC AAGATTTTGC ACAACGACCC AGAAGTGGAG 
AAGAAGCAGG GCTGGGTCAA CGTGGGCGAC CTGGAGGGCT GCGTTCACTA CAAAGTCGTA 
AAGTACGAGA GGATCAAGTT CCTGGTTCTG GCTTTGAAGA ACGCTGTGGA GGTGTACGCC 
TGGGCGCCCA AACCCTACCA CAAGTTCATG GCCTTCAAGT CGTTTGGTGA CCTGGTGCAC 
AAACCTCTGC TGGTGGACCT GACTGTGGAG GAAGGCCAAA GGTTAAAGGT CATCTACGGA 
TCCTGCTCGG GCTTCCACGC TGTGGATGTG GACTCGGGCG CCGTCTACGA CATCTACCTC 
CCCACACATA TCCAGACCAG CATCCAGTGC CACGCCATCA TCATCCTACC CAACACTGAC 
GGCATCGAGC TGCTGGTCTG TTACGAGGAC GAGGGCGTCT ACGTCAACAC GTACGGGCGC 
ATCACCAAAG ACGTGGTGCT GCAGTGGGGA GAAATGCCGA CTTCCGTGGC CTACATTAGG 
TCAAACCAGA TCATGGGCTG GGGCGAAAAG GCCATCGAGA TCCGCTCGGT GGAGACGGGA 
CACCTGGACG GCGTCTTCAT GCACAAGCGA GCCCAGAGAC TCAAATTCCT CTGTGAAAGA 
AACGACAAGG TCTTTTTCGC CTCCGTGCGA GCCGGAGGCG CCAGTCAAGT ATACTTCATG 
ACGCTGGGAC GCTCTTCCCT CATGAGCTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101174648 ( XM_023965940.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023821708.1 Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X3, mRNA.

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CloneID	OJa112415
Clone ID Related Accession (Same CDS sequence)	XM_023965940.1
Accession Version	XM_023965940.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCAGC TAAAAGTCTG GTAGAGATAG ACTTGGCTTC ATTGCGGGAA 
CCAGCTGGGA TATTCGAGCT GGTGGAGGTG GTTGGAAATG GAACCTATGG ACAAGTCTAC 
AAGGGGCGAC ATGTCAAGAC TGGACAACTG GCTGCCATCA AAGTTATGGA TGTCACAGAA 
GATGAGGAGG AGGAAATCAA ACTGGAGATC AATATGCTGA AGAAATATTC CCACCACAGA 
AACATAGCCA CCTATTATGG AGCTTTTATC AAGAAGAGCC CCCCGGGTCA CGATGACCAG 
CTGTGGCTGG TAATGGAGTT CTGTGGTGCC GGTTCGATCA CAGATCTGGT GAAGAACACG 
AAGGGAAACC AGCTGAAGGA AGACTGGATC GCTTACATCT CCAGAGAGAT CCTCAGAGGC 
CTTGCTCACC TACACGCCCA CCACGTGATC CACCGTGACA TTAAAGGCCA GAACGTTCTG 
CTGACAGAGA ACGCTGAAGT TAAACTGGTG GACTTCGGCG TGAGCGCCCA GCTGGATCGC 
ACAGTGGGGC GGAGAAACAC CTTCATCGGG ACTCCATATT GGATGGCGCC TGAGGTCATC 
GCTTGTGATG AGAACCCGGA TGCAACCTAT GATTACAGAA GCGATCTGTG GTCTTGCGGC 
ATCACAGCCA TCGAAATGGC CGAAGGAGCG CCGCCCCTTT GTGACATGCA CCCCATGCGT 
GCGCTCTTCC TCATTCCACG AAACCCCCCA CCGAGGCTCA AATCCAAGAA ATGGTCCAAG 
AAGTTTTTCA GCTTCATCGA GAGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCCCCACA 
GACCAGCTGC TGAAGCACCC GTTCATCCGA GACCAGCCCA ACGAGAGGCA GGTGCGCATC 
CAGCTCAAGG ACCACATCGA CCGGACCAAG AAGAAGAGGG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGATGAGGAG GAGGATCCTC CAGAGCAGGA AGGAGAACCG 
AGCTCCATCA TCAACGCAAC CGAGTCCACC CTGCGACGCG ACTTCATCCG CCTGCAGCAG 
GAGAACAAGG AGCGCTCCGA GGCCATGCGT CACCAGCAGC TCCTCCAGGA GCAGCAGATG 
CGGGAGCAAG AGGAGTACAA GCGGCAGCTC CTGGCAGAGA GGCAGAAGCG CATCGAGCAG 
CAGAAGGAGC AGAGGCGGCG GCTGGAGGAG CAACAACGAT GCGAACGGGA AATGAGGAGG 
CAGCAGGAGC GCGAGCAGCG GCGGCGCGAG CAGGAGGAGA AAAGACGAAT GGAGGAGATG 
GACCGCAGGC GAAAAGAGGA AGAGGAGCGC AGACGCGCCG AAGACGAAAA GAGAAGGAAC 
GATCGGGAGC AGGAGTACAT TAGACGGCAG CTGGAGGAGG AGCAGCGACA CCTGGAGAAG 
CTGCAGGAGC AGCTGCTGCG AGAGCAGGCC ATGCTGCTGG AGTTCAAGTG GCGGGAGCTG 
GACGAGCAGC GCAAAGCCGA GAGACTGCAT AAGCGGCTGC AGCTGGAGCA GGTGTACCTG 
CTGTCCCTGC AGCATGAATC CAAGCAGCAG CACCCTGCTG ACAGGACAGC CCCCTCCTCA 
GAGCCCAGCC ACACTCCCCA GGCTTTGACT CTGCCCCCGG CCTTCTTGCC GACCCCACGG 
GCTCAGGTCA TCGACGCTGT CGTTTCTCTG GCCTCTGAGC CCCCCAGAGT CCCCCAGATT 
ACCCCCTCAG ACAGGACAGT GCCTGAGAAG ACTGACGAGA CCAGTACCAG ACAGATTTCT 
CCCTCCCAAA CCCCCCAGCC CTCTGTAAGC GAAACCCCCA CCGACTCTAA GAGTCCCAAG 
GCAGAAAGTC TGGAGCCTGA CAGGATCTCC GAGCCTGTCA GGCTTCCCAT TCAGCCTATC 
AGAGAGGCGG ATGAGCGCTA CCGTAAAAAC ATCCAGGGCT CCCCGCAGGC CGCCCCTCCT 
CCCAAGCAGC CCCCTCTGCC ACCCCGATCC TCCGAACCCT TCTCCAACGG CGGCTCCTCC 
TCTGAGGCCA TGCACCGGCC CATGGAGCCT CAGGTCCAGT GGTCACACCT GGCCGCTCTT 
AAGAGCAGCA ACAGCGCCAC CCCCTCTCCT CCTCCGCCTG TTGTCGCTCG CTCCCAGTCC 
TTCAGCGAAT CCGGTGGCGT GGCGTCTAAC TTTGCACAAC TCCACCTGCG CTCCCAGGAC 
CCCCACCATC AACACCACCA CCACCCATCG CCTGCATTCA CTGACCCCCA CCCCCAACCT 
CCCCTCCACC ACCCCCAGGC TCAGAATAGG CTGGAACACC AGTCCAGCAG TGAAGAGGTT 
CCTCCAAAGG TACCAGTGAG GACAACATCC AGATCTCCGG TTTTGTCGCG GCGAGAGTCT 
CCTCTGCCAA CGCAGCCCAA CAACCAGAGG AACGCCGTCG GGAACATGGA GCAGCGCCCT 
CTATGGGACA GGGTGGAGAA GCTGCAGTCC CGACCCGGCA GCGGCAGCTC CTCCAACTCC 
AACTCTCAGG CCAGTCCCGG CGAGCGTTTC AGACCTCGCT GTGAGTCCCC TGCTTCCTCT 
AAATCTGAGG GATCGCCTCT CCAGCGGCCC GAAAACCTCC CCAAAAAACA GGACGAAAGG 
AACGTGGCCC GGCCGACGCG ACCCGCTGAC CTGACGGCGC TGGCTAAGGA GCTGCGTGCC 
GTGGACGACG TGCGGCCCCC CCACAAGGTC ACAGACTACT CCTCATCGAG CGATGACTCG 
GGCACCACGG ATGAGGAAGA CGACGAGGAG GTGGACCAGG AGCCAGGGGA CGAGTCCACG 
TCGGGGGCCG AGGACTCCAG AGCTGGGAGG CTCAGAAACG GGGAGGCAGA CTCTGCTAAG 
ACCATGCTGG TGGAGGACTC TGAGAGTGAG CAGGGCACCA CCCCCTCCAA AGACGGGACC 
CTGGTCATCA GACAGTCGCA GTCAGAGAGC AACTCCATGT CCAAACACAA GTCTTCCTCT 
TCGTTCACTC CCTTCATCGA CCCACGCCTC CTCCAGATCT CTCCATCAGG CGGCAGCTCC 
CTCAACAACA TGGCGGTTTT TGGACAAGAA GGCCGACCCG CCGACCCACT GCGCTCTGAC 
CCCTCCCGTA AAGGCTCGGT GGTCAACGTC AACCCTGTCA ACACTCGTCC GCCCAGCGAC 
ACTCCCGAGA TCCGCAAGTA CAAGAAGAGG TTCAACTCCG AGATTCTGTG CGCGGCGCTC 
TGGGGGGTGA ACCTGCTGGT GGGGACAGAA AGCGGGCTGA TGCTGCTGGA CCGGAGCGGT 
CAGGGTAAGG TTTACCCGCT GATTAATCGA CGCCGCATCC AGCAGATGGA TGTCCTGGAG 
GGACTCAACG TCCTGGTAAC CATATCAGGT AAAAAGAACA AACTGCGAGT GTATTACCTG 
TCGTGGCTCC GAAACAAGAT TTTGCACAAC GACCCAGAAG TGGAGAAGAA GCAGGGCTGG 
GTCAACGTGG GCGACCTGGA GGGCTGCGTT CACTACAAAG TCGTAAAGTA CGAGAGGATC 
AAGTTCCTGG TTCTGGCTTT GAAGAACGCT GTGGAGGTGT ACGCCTGGGC GCCCAAACCC 
TACCACAAGT TCATGGCCTT CAAGTCGTTT GGTGACCTGG TGCACAAACC TCTGCTGGTG 
GACCTGACTG TGGAGGAAGG CCAAAGGTTA AAGGTCATCT ACGGATCCTG CTCGGGCTTC 
CACGCTGTGG ATGTGGACTC GGGCGCCGTC TACGACATCT ACCTCCCCAC ACATATCCAG 
ACCAGCATCC AGTGCCACGC CATCATCATC CTACCCAACA CTGACGGCAT CGAGCTGCTG 
GTCTGTTACG AGGACGAGGG CGTCTACGTC AACACGTACG GGCGCATCAC CAAAGACGTG 
GTGCTGCAGT GGGGAGAAAT GCCGACTTCC GTGGCCTACA TTAGGTCAAA CCAGATCATG 
GGCTGGGGCG AAAAGGCCAT CGAGATCCGC TCGGTGGAGA CGGGACACCT GGACGGCGTC 
TTCATGCACA AGCGAGCCCA GAGACTCAAA TTCCTCTGTG AAAGAAACGA CAAGGTCTTT 
TTCGCCTCCG TGCGAGCCGG AGGCGCCAGT CAAGTATACT TCATGACGCT GGGACGCTCT 
TCCCTCATGA GCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023821708.1
CDS	220..4377
Translation	MANDSPAKSLVEIDLASLREPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNQLKEDWIAYISREILRGLAHLHAHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIESCLVKNYMQRPPTDQLLKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEDEEEDPPEQEGEPSSIINATESTLRRDFIRLQQENKERSEAMRHQQLLQEQQMREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRCEREMRRQQEREQRRREQEEKRRMEEMDRRRKEEEERRRAEDEKRRNDREQEYIRRQLEEEQRHLEKLQEQLLREQAMLLEFKWRELDEQRKAERLHKRLQLEQVYLLSLQHESKQQHPADRTAPSSEPSHTPQALTLPPAFLPTPRAQVIDAVVSLASEPPRVPQITPSDRTVPEKTDETSTRQISPSQTPQPSVSETPTDSKSPKAESLEPDRISEPVRLPIQPIREADERYRKNIQGSPQAAPPPKQPPLPPRSSEPFSNGGSSSEAMHRPMEPQVQWSHLAALKSSNSATPSPPPPVVARSQSFSESGGVASNFAQLHLRSQDPHHQHHHHPSPAFTDPHPQPPLHHPQAQNRLEHQSSSEEVPPKVPVRTTSRSPVLSRRESPLPTQPNNQRNAVGNMEQRPLWDRVEKLQSRPGSGSSSNSNSQASPGERFRPRCESPASSKSEGSPLQRPENLPKKQDERNVARPTRPADLTALAKELRAVDDVRPPHKVTDYSSSSDDSGTTDEEDDEEVDQEPGDESTSGAEDSRAGRLRNGEADSAKTMLVEDSESEQGTTPSKDGTLVIRQSQSESNSMSKHKSSSSFTPFIDPRLLQISPSGGSSLNNMAVFGQEGRPADPLRSDPSRKGSVVNVNPVNTRPPSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRIQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNKILHNDPEVEKKQGWVNVGDLEGCVHYKVVKYERIKFLVLALKNAVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCSGFHAVDVDSGAVYDIYLPTHIQTSIQCHAIIILPNTDGIELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRAGGASQVYFMTLGRSSLMSW

Target ORF information:

RefSeq Version	XM_023965940.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023965940.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCAGC TAAAAGTCTG GTAGAGATAG ACTTGGCTTC ATTGCGGGAA 
CCAGCTGGGA TATTCGAGCT GGTGGAGGTG GTTGGAAATG GAACCTATGG ACAAGTCTAC 
AAGGGGCGAC ATGTCAAGAC TGGACAACTG GCTGCCATCA AAGTTATGGA TGTCACAGAA 
GATGAGGAGG AGGAAATCAA ACTGGAGATC AATATGCTGA AGAAATATTC CCACCACAGA 
AACATAGCCA CCTATTATGG AGCTTTTATC AAGAAGAGCC CCCCGGGTCA CGATGACCAG 
CTGTGGCTGG TAATGGAGTT CTGTGGTGCC GGTTCGATCA CAGATCTGGT GAAGAACACG 
AAGGGAAACC AGCTGAAGGA AGACTGGATC GCTTACATCT CCAGAGAGAT CCTCAGAGGC 
CTTGCTCACC TACACGCCCA CCACGTGATC CACCGTGACA TTAAAGGCCA GAACGTTCTG 
CTGACAGAGA ACGCTGAAGT TAAACTGGTG GACTTCGGCG TGAGCGCCCA GCTGGATCGC 
ACAGTGGGGC GGAGAAACAC CTTCATCGGG ACTCCATATT GGATGGCGCC TGAGGTCATC 
GCTTGTGATG AGAACCCGGA TGCAACCTAT GATTACAGAA GCGATCTGTG GTCTTGCGGC 
ATCACAGCCA TCGAAATGGC CGAAGGAGCG CCGCCCCTTT GTGACATGCA CCCCATGCGT 
GCGCTCTTCC TCATTCCACG AAACCCCCCA CCGAGGCTCA AATCCAAGAA ATGGTCCAAG 
AAGTTTTTCA GCTTCATCGA GAGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCCCCACA 
GACCAGCTGC TGAAGCACCC GTTCATCCGA GACCAGCCCA ACGAGAGGCA GGTGCGCATC 
CAGCTCAAGG ACCACATCGA CCGGACCAAG AAGAAGAGGG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGATGAGGAG GAGGATCCTC CAGAGCAGGA AGGAGAACCG 
AGCTCCATCA TCAACGCAAC CGAGTCCACC CTGCGACGCG ACTTCATCCG CCTGCAGCAG 
GAGAACAAGG AGCGCTCCGA GGCCATGCGT CACCAGCAGC TCCTCCAGGA GCAGCAGATG 
CGGGAGCAAG AGGAGTACAA GCGGCAGCTC CTGGCAGAGA GGCAGAAGCG CATCGAGCAG 
CAGAAGGAGC AGAGGCGGCG GCTGGAGGAG CAACAACGAT GCGAACGGGA AATGAGGAGG 
CAGCAGGAGC GCGAGCAGCG GCGGCGCGAG CAGGAGGAGA AAAGACGAAT GGAGGAGATG 
GACCGCAGGC GAAAAGAGGA AGAGGAGCGC AGACGCGCCG AAGACGAAAA GAGAAGGAAC 
GATCGGGAGC AGGAGTACAT TAGACGGCAG CTGGAGGAGG AGCAGCGACA CCTGGAGAAG 
CTGCAGGAGC AGCTGCTGCG AGAGCAGGCC ATGCTGCTGG AGTTCAAGTG GCGGGAGCTG 
GACGAGCAGC GCAAAGCCGA GAGACTGCAT AAGCGGCTGC AGCTGGAGCA GGTGTACCTG 
CTGTCCCTGC AGCATGAATC CAAGCAGCAG CACCCTGCTG ACAGGACAGC CCCCTCCTCA 
GAGCCCAGCC ACACTCCCCA GGCTTTGACT CTGCCCCCGG CCTTCTTGCC GACCCCACGG 
GCTCAGGTCA TCGACGCTGT CGTTTCTCTG GCCTCTGAGC CCCCCAGAGT CCCCCAGATT 
ACCCCCTCAG ACAGGACAGT GCCTGAGAAG ACTGACGAGA CCAGTACCAG ACAGATTTCT 
CCCTCCCAAA CCCCCCAGCC CTCTGTAAGC GAAACCCCCA CCGACTCTAA GAGTCCCAAG 
GCAGAAAGTC TGGAGCCTGA CAGGATCTCC GAGCCTGTCA GGCTTCCCAT TCAGCCTATC 
AGAGAGGCGG ATGAGCGCTA CCGTAAAAAC ATCCAGGGCT CCCCGCAGGC CGCCCCTCCT 
CCCAAGCAGC CCCCTCTGCC ACCCCGATCC TCCGAACCCT TCTCCAACGG CGGCTCCTCC 
TCTGAGGCCA TGCACCGGCC CATGGAGCCT CAGGTCCAGT GGTCACACCT GGCCGCTCTT 
AAGAGCAGCA ACAGCGCCAC CCCCTCTCCT CCTCCGCCTG TTGTCGCTCG CTCCCAGTCC 
TTCAGCGAAT CCGGTGGCGT GGCGTCTAAC TTTGCACAAC TCCACCTGCG CTCCCAGGAC 
CCCCACCATC AACACCACCA CCACCCATCG CCTGCATTCA CTGACCCCCA CCCCCAACCT 
CCCCTCCACC ACCCCCAGGC TCAGAATAGG CTGGAACACC AGTCCAGCAG TGAAGAGGTT 
CCTCCAAAGG TACCAGTGAG GACAACATCC AGATCTCCGG TTTTGTCGCG GCGAGAGTCT 
CCTCTGCCAA CGCAGCCCAA CAACCAGAGG AACGCCGTCG GGAACATGGA GCAGCGCCCT 
CTATGGGACA GGGTGGAGAA GCTGCAGTCC CGACCCGGCA GCGGCAGCTC CTCCAACTCC 
AACTCTCAGG CCAGTCCCGG CGAGCGTTTC AGACCTCGCT GTGAGTCCCC TGCTTCCTCT 
AAATCTGAGG GATCGCCTCT CCAGCGGCCC GAAAACCTCC CCAAAAAACA GGACGAAAGG 
AACGTGGCCC GGCCGACGCG ACCCGCTGAC CTGACGGCGC TGGCTAAGGA GCTGCGTGCC 
GTGGACGACG TGCGGCCCCC CCACAAGGTC ACAGACTACT CCTCATCGAG CGATGACTCG 
GGCACCACGG ATGAGGAAGA CGACGAGGAG GTGGACCAGG AGCCAGGGGA CGAGTCCACG 
TCGGGGGCCG AGGACTCCAG AGCTGGGAGG CTCAGAAACG GGGAGGCAGA CTCTGCTAAG 
ACCATGCTGG TGGAGGACTC TGAGAGTGAG CAGGGCACCA CCCCCTCCAA AGACGGGACC 
CTGGTCATCA GACAGTCGCA GTCAGAGAGC AACTCCATGT CCAAACACAA GTCTTCCTCT 
TCGTTCACTC CCTTCATCGA CCCACGCCTC CTCCAGATCT CTCCATCAGG CGGCAGCTCC 
CTCAACAACA TGGCGGTTTT TGGACAAGAA GGCCGACCCG CCGACCCACT GCGCTCTGAC 
CCCTCCCGTA AAGGCTCGGT GGTCAACGTC AACCCTGTCA ACACTCGTCC GCCCAGCGAC 
ACTCCCGAGA TCCGCAAGTA CAAGAAGAGG TTCAACTCCG AGATTCTGTG CGCGGCGCTC 
TGGGGGGTGA ACCTGCTGGT GGGGACAGAA AGCGGGCTGA TGCTGCTGGA CCGGAGCGGT 
CAGGGTAAGG TTTACCCGCT GATTAATCGA CGCCGCATCC AGCAGATGGA TGTCCTGGAG 
GGACTCAACG TCCTGGTAAC CATATCAGGT AAAAAGAACA AACTGCGAGT GTATTACCTG 
TCGTGGCTCC GAAACAAGAT TTTGCACAAC GACCCAGAAG TGGAGAAGAA GCAGGGCTGG 
GTCAACGTGG GCGACCTGGA GGGCTGCGTT CACTACAAAG TCGTAAAGTA CGAGAGGATC 
AAGTTCCTGG TTCTGGCTTT GAAGAACGCT GTGGAGGTGT ACGCCTGGGC GCCCAAACCC 
TACCACAAGT TCATGGCCTT CAAGTCGTTT GGTGACCTGG TGCACAAACC TCTGCTGGTG 
GACCTGACTG TGGAGGAAGG CCAAAGGTTA AAGGTCATCT ACGGATCCTG CTCGGGCTTC 
CACGCTGTGG ATGTGGACTC GGGCGCCGTC TACGACATCT ACCTCCCCAC ACATATCCAG 
ACCAGCATCC AGTGCCACGC CATCATCATC CTACCCAACA CTGACGGCAT CGAGCTGCTG 
GTCTGTTACG AGGACGAGGG CGTCTACGTC AACACGTACG GGCGCATCAC CAAAGACGTG 
GTGCTGCAGT GGGGAGAAAT GCCGACTTCC GTGGCCTACA TTAGGTCAAA CCAGATCATG 
GGCTGGGGCG AAAAGGCCAT CGAGATCCGC TCGGTGGAGA CGGGACACCT GGACGGCGTC 
TTCATGCACA AGCGAGCCCA GAGACTCAAA TTCCTCTGTG AAAGAAACGA CAAGGTCTTT 
TTCGCCTCCG TGCGAGCCGG AGGCGCCAGT CAAGTATACT TCATGACGCT GGGACGCTCT 
TCCCTCATGA GCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101174648 ( XM_023965943.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023821711.1 Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X4, mRNA.

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CloneID	OJa112416
Clone ID Related Accession (Same CDS sequence)	XM_023965943.1
Accession Version	XM_023965943.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCAGC TAAAAGTCTG GTAGAGATAG ACTTGGCTTC ATTGCGGGAA 
CCAGCTGGGA TATTCGAGCT GGTGGAGGTG GTTGGAAATG GAACCTATGG ACAAGTCTAC 
AAGGGGCGAC ATGTCAAGAC TGGACAACTG GCTGCCATCA AAGTTATGGA TGTCACAGAA 
GATGAGGAGG AGGAAATCAA ACTGGAGATC AATATGCTGA AGAAATATTC CCACCACAGA 
AACATAGCCA CCTATTATGG AGCTTTTATC AAGAAGAGCC CCCCGGGTCA CGATGACCAG 
CTGTGGCTGG TAATGGAGTT CTGTGGTGCC GGTTCGATCA CAGATCTGGT GAAGAACACG 
AAGGGAAACC AGCTGAAGGA AGACTGGATC GCTTACATCT CCAGAGAGAT CCTCAGAGGC 
CTTGCTCACC TACACGCCCA CCACGTGATC CACCGTGACA TTAAAGGCCA GAACGTTCTG 
CTGACAGAGA ACGCTGAAGT TAAACTGGTG GACTTCGGCG TGAGCGCCCA GCTGGATCGC 
ACAGTGGGGC GGAGAAACAC CTTCATCGGG ACTCCATATT GGATGGCGCC TGAGGTCATC 
GCTTGTGATG AGAACCCGGA TGCAACCTAT GATTACAGAA GCGATCTGTG GTCTTGCGGC 
ATCACAGCCA TCGAAATGGC CGAAGGAGCG CCGCCCCTTT GTGACATGCA CCCCATGCGT 
GCGCTCTTCC TCATTCCACG AAACCCCCCA CCGAGGCTCA AATCCAAGAA ATGGTCCAAG 
AAGTTTTTCA GCTTCATCGA GAGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCCCCACA 
GACCAGCTGC TGAAGCACCC GTTCATCCGA GACCAGCCCA ACGAGAGGCA GGTGCGCATC 
CAGCTCAAGG ACCACATCGA CCGGACCAAG AAGAAGAGGG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGATGAGGAG GAGGATCCTC CAGAGCAGGA AGGAGAACCG 
AGCTCCATCA TCAACGCAAC CGAGTCCACC CTGCGACGCG ACTTCATCCG CCTGCAGCAG 
GAGAACAAGG AGCGCTCCGA GGCCATGCGT CACCAGCAGC TCCTCCAGGA GCAGCAGATG 
CGGGAGCAAG AGGAGTACAA GCGGCAGCTC CTGGCAGAGA GGCAGAAGCG CATCGAGCAG 
CAGAAGGAGC AGAGGCGGCG GCTGGAGGAG CAACAACGAT GCGAACGGGA AATGAGGAGG 
CAGCAGGAGC GCGAGCAGCG GCGGCGCGAG CAGGAGGAGA AAAGACGAAT GGAGGAGATG 
GACCGCAGGC GAAAAGAGGA AGAGGAGCGC AGACGCGCCG AAGACGAAAA GAGAAGGAAC 
GATCGGGAGC AGGAGTACAT TAGACGGCAG CTGGAGGAGG AGCAGCGACA CCTGGAGAAG 
CTGCAGGAGC AGCTGCTGCG AGAGCAGGCC ATGCTGCTGG AGTTCAAGTG GCGGGAGCTG 
GACGAGCAGC GCAAAGCCGA GAGACTGCAT AAGCGGCTGC AGCTGGAGCA GGTGTACCTG 
CTGTCCCTGC AGCATGAATC CAAGCAGCAG CACCCTGCTG ACAGGACAGC CCCCTCCTCA 
GAGCCCAGCC ACACTCCCCA GGCTTTGACT CTGCCCCCGG CCTTCTTGCC GACCCCACGG 
GCTCAGGTCA TCGACGCTGT CGTTTCTCTG GCCTCTGAGC CCCCCAGAGT CCCCCAGATT 
ACCCCCTCAG ACAGGACAGT GCCTGAGAAG ACTGACGAGA CCAGTACCAG ACAGATTTCT 
CCCTCCCAAA CCCCCCAGCC CTCTGTAAGC GAAACCCCCA CCGACTCTAA GAGTCCCAAG 
GCAGAAAGTC TGGAGCCTGA CAGGATCTCC GAGCCTGTCA GGCTTCCCAT TCAGCCTATC 
AGAGAGGCGG ATGAGCGCTA CCGTAAAAAC ATCCAGGGCT CCCCGCAGGC CGCCCCTCCT 
CCCAAGCAGC CCCCTCTGCC ACCCCGATCC TCCGAACCCT TCTCCAACGG CGGCTCCTCC 
TCTGAGGCCA TGCACCGGCC CATGGAGCCT CAGGTACCAG TGAGGACAAC ATCCAGATCT 
CCGGTTTTGT CGCGGCGAGA GTCTCCTCTG CCAACGCAGC CCAACAACCA GAGGAACGCC 
GTCGGGAACA TGGAGCAGCG CCCTCTATGG GACAGGGTGG AGAAGCTGCA GTCCCGACCC 
GGCAGCGGCA GCTCCTCCAA CTCCAACTCT CAGGCCAGTC CCGGCGAGCG TTTCAGACCT 
CGCTGTGAGT CCCCTGCTTC CTCTAAATCT GAGGGATCGC CTCTCCAGCG GCCCGAAAAC 
CTCCCCAAAA AACAGGACGA AAGGAACGTG GCCCGGCCGA CGCGACCCGC TGACCTGACG 
GCGCTGGCTA AGGAGCTGCG TGCCGTGGAC GACGTGCGGC CCCCCCACAA GGTCACAGAC 
TACTCCTCAT CGAGCGATGA CTCGGGCACC ACGGATGAGG AAGACGACGA GGAGGTGGAC 
CAGGAGCCAG GGGACGAGTC CACGTCGGGG GCCGAGGACT CCAGAGCTGG GAGGCTCAGA 
AACGGGGAGG CAGACTCTGC TAAGACCATG CTGGTGGAGG ACTCTGAGAG TGAGCAGGGC 
ACCACCCCCT CCAAAGACGG GACCCTGGTC ATCAGACAGA GCACAGCTGA CATAAAGCGG 
TTGGTCAATG TCTCGTCTTC ATCATCCTCC TCCTCGCCCG GCTCCGGTCG TGGCCCCCCA 
GAGAAAAACG GCCGCATGTA CCACCTCCCC GACATTATTC AGCAGAGCCA TCATTCCTCC 
CCCCCCTCCA CAGCCAGCTT CTCCTCCTTG TCCTCCTCCT CCTCCTCCTC CTTCCCCTCA 
TCATCTAGCC AGGCCAGTCT TGGCGTCTCC TCACAGATCT CCTTGGAGGA GCTCGTAGCC 
GTAGAGTCGC AGTCAGAGAG CAACTCCATG TCCAAACACA AGTCTTCCTC TTCGTTCACT 
CCCTTCATCG ACCCACGCCT CCTCCAGATC TCTCCATCAG GCGGCAGCTC CCTCAACAAC 
ATGGCGGTTT TTGGACAAGA AGGCCGACCC GCCGACCCAC TGCGCTCTGA CCCCTCCCGT 
AAAGGCTCGG TGGTCAACGT CAACCCTGTC AACACTCGTC CGCCCAGCGA CACTCCCGAG 
ATCCGCAAGT ACAAGAAGAG GTTCAACTCC GAGATTCTGT GCGCGGCGCT CTGGGGGGTG 
AACCTGCTGG TGGGGACAGA AAGCGGGCTG ATGCTGCTGG ACCGGAGCGG TCAGGGTAAG 
GTTTACCCGC TGATTAATCG ACGCCGCATC CAGCAGATGG ATGTCCTGGA GGGACTCAAC 
GTCCTGGTAA CCATATCAGG TAAAAAGAAC AAACTGCGAG TGTATTACCT GTCGTGGCTC 
CGAAACAAGA TTTTGCACAA CGACCCAGAA GTGGAGAAGA AGCAGGGCTG GGTCAACGTG 
GGCGACCTGG AGGGCTGCGT TCACTACAAA GTCGTAAAGT ACGAGAGGAT CAAGTTCCTG 
GTTCTGGCTT TGAAGAACGC TGTGGAGGTG TACGCCTGGG CGCCCAAACC CTACCACAAG 
TTCATGGCCT TCAAGTCGTT TGGTGACCTG GTGCACAAAC CTCTGCTGGT GGACCTGACT 
GTGGAGGAAG GCCAAAGGTT AAAGGTCATC TACGGATCCT GCTCGGGCTT CCACGCTGTG 
GATGTGGACT CGGGCGCCGT CTACGACATC TACCTCCCCA CACATATCCA GACCAGCATC 
CAGTGCCACG CCATCATCAT CCTACCCAAC ACTGACGGCA TCGAGCTGCT GGTCTGTTAC 
GAGGACGAGG GCGTCTACGT CAACACGTAC GGGCGCATCA CCAAAGACGT GGTGCTGCAG 
TGGGGAGAAA TGCCGACTTC CGTGGCCTAC ATTAGGTCAA ACCAGATCAT GGGCTGGGGC 
GAAAAGGCCA TCGAGATCCG CTCGGTGGAG ACGGGACACC TGGACGGCGT CTTCATGCAC 
AAGCGAGCCC AGAGACTCAA ATTCCTCTGT GAAAGAAACG ACAAGGTCTT TTTCGCCTCC 
GTGCGAGCCG GAGGCGCCAG TCAAGTATAC TTCATGACGC TGGGACGCTC TTCCCTCATG 
AGCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023821711.1
CDS	220..4368
Translation	MANDSPAKSLVEIDLASLREPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNQLKEDWIAYISREILRGLAHLHAHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIESCLVKNYMQRPPTDQLLKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEDEEEDPPEQEGEPSSIINATESTLRRDFIRLQQENKERSEAMRHQQLLQEQQMREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRCEREMRRQQEREQRRREQEEKRRMEEMDRRRKEEEERRRAEDEKRRNDREQEYIRRQLEEEQRHLEKLQEQLLREQAMLLEFKWRELDEQRKAERLHKRLQLEQVYLLSLQHESKQQHPADRTAPSSEPSHTPQALTLPPAFLPTPRAQVIDAVVSLASEPPRVPQITPSDRTVPEKTDETSTRQISPSQTPQPSVSETPTDSKSPKAESLEPDRISEPVRLPIQPIREADERYRKNIQGSPQAAPPPKQPPLPPRSSEPFSNGGSSSEAMHRPMEPQVPVRTTSRSPVLSRRESPLPTQPNNQRNAVGNMEQRPLWDRVEKLQSRPGSGSSSNSNSQASPGERFRPRCESPASSKSEGSPLQRPENLPKKQDERNVARPTRPADLTALAKELRAVDDVRPPHKVTDYSSSSDDSGTTDEEDDEEVDQEPGDESTSGAEDSRAGRLRNGEADSAKTMLVEDSESEQGTTPSKDGTLVIRQSTADIKRLVNVSSSSSSSSPGSGRGPPEKNGRMYHLPDIIQQSHHSSPPSTASFSSLSSSSSSSFPSSSSQASLGVSSQISLEELVAVESQSESNSMSKHKSSSSFTPFIDPRLLQISPSGGSSLNNMAVFGQEGRPADPLRSDPSRKGSVVNVNPVNTRPPSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRIQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNKILHNDPEVEKKQGWVNVGDLEGCVHYKVVKYERIKFLVLALKNAVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCSGFHAVDVDSGAVYDIYLPTHIQTSIQCHAIIILPNTDGIELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRAGGASQVYFMTLGRSSLMSW

Target ORF information:

RefSeq Version	XM_023965943.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023965943.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCAGC TAAAAGTCTG GTAGAGATAG ACTTGGCTTC ATTGCGGGAA 
CCAGCTGGGA TATTCGAGCT GGTGGAGGTG GTTGGAAATG GAACCTATGG ACAAGTCTAC 
AAGGGGCGAC ATGTCAAGAC TGGACAACTG GCTGCCATCA AAGTTATGGA TGTCACAGAA 
GATGAGGAGG AGGAAATCAA ACTGGAGATC AATATGCTGA AGAAATATTC CCACCACAGA 
AACATAGCCA CCTATTATGG AGCTTTTATC AAGAAGAGCC CCCCGGGTCA CGATGACCAG 
CTGTGGCTGG TAATGGAGTT CTGTGGTGCC GGTTCGATCA CAGATCTGGT GAAGAACACG 
AAGGGAAACC AGCTGAAGGA AGACTGGATC GCTTACATCT CCAGAGAGAT CCTCAGAGGC 
CTTGCTCACC TACACGCCCA CCACGTGATC CACCGTGACA TTAAAGGCCA GAACGTTCTG 
CTGACAGAGA ACGCTGAAGT TAAACTGGTG GACTTCGGCG TGAGCGCCCA GCTGGATCGC 
ACAGTGGGGC GGAGAAACAC CTTCATCGGG ACTCCATATT GGATGGCGCC TGAGGTCATC 
GCTTGTGATG AGAACCCGGA TGCAACCTAT GATTACAGAA GCGATCTGTG GTCTTGCGGC 
ATCACAGCCA TCGAAATGGC CGAAGGAGCG CCGCCCCTTT GTGACATGCA CCCCATGCGT 
GCGCTCTTCC TCATTCCACG AAACCCCCCA CCGAGGCTCA AATCCAAGAA ATGGTCCAAG 
AAGTTTTTCA GCTTCATCGA GAGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCCCCACA 
GACCAGCTGC TGAAGCACCC GTTCATCCGA GACCAGCCCA ACGAGAGGCA GGTGCGCATC 
CAGCTCAAGG ACCACATCGA CCGGACCAAG AAGAAGAGGG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGATGAGGAG GAGGATCCTC CAGAGCAGGA AGGAGAACCG 
AGCTCCATCA TCAACGCAAC CGAGTCCACC CTGCGACGCG ACTTCATCCG CCTGCAGCAG 
GAGAACAAGG AGCGCTCCGA GGCCATGCGT CACCAGCAGC TCCTCCAGGA GCAGCAGATG 
CGGGAGCAAG AGGAGTACAA GCGGCAGCTC CTGGCAGAGA GGCAGAAGCG CATCGAGCAG 
CAGAAGGAGC AGAGGCGGCG GCTGGAGGAG CAACAACGAT GCGAACGGGA AATGAGGAGG 
CAGCAGGAGC GCGAGCAGCG GCGGCGCGAG CAGGAGGAGA AAAGACGAAT GGAGGAGATG 
GACCGCAGGC GAAAAGAGGA AGAGGAGCGC AGACGCGCCG AAGACGAAAA GAGAAGGAAC 
GATCGGGAGC AGGAGTACAT TAGACGGCAG CTGGAGGAGG AGCAGCGACA CCTGGAGAAG 
CTGCAGGAGC AGCTGCTGCG AGAGCAGGCC ATGCTGCTGG AGTTCAAGTG GCGGGAGCTG 
GACGAGCAGC GCAAAGCCGA GAGACTGCAT AAGCGGCTGC AGCTGGAGCA GGTGTACCTG 
CTGTCCCTGC AGCATGAATC CAAGCAGCAG CACCCTGCTG ACAGGACAGC CCCCTCCTCA 
GAGCCCAGCC ACACTCCCCA GGCTTTGACT CTGCCCCCGG CCTTCTTGCC GACCCCACGG 
GCTCAGGTCA TCGACGCTGT CGTTTCTCTG GCCTCTGAGC CCCCCAGAGT CCCCCAGATT 
ACCCCCTCAG ACAGGACAGT GCCTGAGAAG ACTGACGAGA CCAGTACCAG ACAGATTTCT 
CCCTCCCAAA CCCCCCAGCC CTCTGTAAGC GAAACCCCCA CCGACTCTAA GAGTCCCAAG 
GCAGAAAGTC TGGAGCCTGA CAGGATCTCC GAGCCTGTCA GGCTTCCCAT TCAGCCTATC 
AGAGAGGCGG ATGAGCGCTA CCGTAAAAAC ATCCAGGGCT CCCCGCAGGC CGCCCCTCCT 
CCCAAGCAGC CCCCTCTGCC ACCCCGATCC TCCGAACCCT TCTCCAACGG CGGCTCCTCC 
TCTGAGGCCA TGCACCGGCC CATGGAGCCT CAGGTACCAG TGAGGACAAC ATCCAGATCT 
CCGGTTTTGT CGCGGCGAGA GTCTCCTCTG CCAACGCAGC CCAACAACCA GAGGAACGCC 
GTCGGGAACA TGGAGCAGCG CCCTCTATGG GACAGGGTGG AGAAGCTGCA GTCCCGACCC 
GGCAGCGGCA GCTCCTCCAA CTCCAACTCT CAGGCCAGTC CCGGCGAGCG TTTCAGACCT 
CGCTGTGAGT CCCCTGCTTC CTCTAAATCT GAGGGATCGC CTCTCCAGCG GCCCGAAAAC 
CTCCCCAAAA AACAGGACGA AAGGAACGTG GCCCGGCCGA CGCGACCCGC TGACCTGACG 
GCGCTGGCTA AGGAGCTGCG TGCCGTGGAC GACGTGCGGC CCCCCCACAA GGTCACAGAC 
TACTCCTCAT CGAGCGATGA CTCGGGCACC ACGGATGAGG AAGACGACGA GGAGGTGGAC 
CAGGAGCCAG GGGACGAGTC CACGTCGGGG GCCGAGGACT CCAGAGCTGG GAGGCTCAGA 
AACGGGGAGG CAGACTCTGC TAAGACCATG CTGGTGGAGG ACTCTGAGAG TGAGCAGGGC 
ACCACCCCCT CCAAAGACGG GACCCTGGTC ATCAGACAGA GCACAGCTGA CATAAAGCGG 
TTGGTCAATG TCTCGTCTTC ATCATCCTCC TCCTCGCCCG GCTCCGGTCG TGGCCCCCCA 
GAGAAAAACG GCCGCATGTA CCACCTCCCC GACATTATTC AGCAGAGCCA TCATTCCTCC 
CCCCCCTCCA CAGCCAGCTT CTCCTCCTTG TCCTCCTCCT CCTCCTCCTC CTTCCCCTCA 
TCATCTAGCC AGGCCAGTCT TGGCGTCTCC TCACAGATCT CCTTGGAGGA GCTCGTAGCC 
GTAGAGTCGC AGTCAGAGAG CAACTCCATG TCCAAACACA AGTCTTCCTC TTCGTTCACT 
CCCTTCATCG ACCCACGCCT CCTCCAGATC TCTCCATCAG GCGGCAGCTC CCTCAACAAC 
ATGGCGGTTT TTGGACAAGA AGGCCGACCC GCCGACCCAC TGCGCTCTGA CCCCTCCCGT 
AAAGGCTCGG TGGTCAACGT CAACCCTGTC AACACTCGTC CGCCCAGCGA CACTCCCGAG 
ATCCGCAAGT ACAAGAAGAG GTTCAACTCC GAGATTCTGT GCGCGGCGCT CTGGGGGGTG 
AACCTGCTGG TGGGGACAGA AAGCGGGCTG ATGCTGCTGG ACCGGAGCGG TCAGGGTAAG 
GTTTACCCGC TGATTAATCG ACGCCGCATC CAGCAGATGG ATGTCCTGGA GGGACTCAAC 
GTCCTGGTAA CCATATCAGG TAAAAAGAAC AAACTGCGAG TGTATTACCT GTCGTGGCTC 
CGAAACAAGA TTTTGCACAA CGACCCAGAA GTGGAGAAGA AGCAGGGCTG GGTCAACGTG 
GGCGACCTGG AGGGCTGCGT TCACTACAAA GTCGTAAAGT ACGAGAGGAT CAAGTTCCTG 
GTTCTGGCTT TGAAGAACGC TGTGGAGGTG TACGCCTGGG CGCCCAAACC CTACCACAAG 
TTCATGGCCT TCAAGTCGTT TGGTGACCTG GTGCACAAAC CTCTGCTGGT GGACCTGACT 
GTGGAGGAAG GCCAAAGGTT AAAGGTCATC TACGGATCCT GCTCGGGCTT CCACGCTGTG 
GATGTGGACT CGGGCGCCGT CTACGACATC TACCTCCCCA CACATATCCA GACCAGCATC 
CAGTGCCACG CCATCATCAT CCTACCCAAC ACTGACGGCA TCGAGCTGCT GGTCTGTTAC 
GAGGACGAGG GCGTCTACGT CAACACGTAC GGGCGCATCA CCAAAGACGT GGTGCTGCAG 
TGGGGAGAAA TGCCGACTTC CGTGGCCTAC ATTAGGTCAA ACCAGATCAT GGGCTGGGGC 
GAAAAGGCCA TCGAGATCCG CTCGGTGGAG ACGGGACACC TGGACGGCGT CTTCATGCAC 
AAGCGAGCCC AGAGACTCAA ATTCCTCTGT GAAAGAAACG ACAAGGTCTT TTTCGCCTCC 
GTGCGAGCCG GAGGCGCCAG TCAAGTATAC TTCATGACGC TGGGACGCTC TTCCCTCATG 
AGCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101174648 ( XM_023965947.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023821715.1 Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OJa112417
Clone ID Related Accession (Same CDS sequence)	XM_023965947.1
Accession Version	XM_023965947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCAGC TAAAAGTCTG GTAGAGATAG ACTTGGCTTC ATTGCGGGAA 
CCAGCTGGGA TATTCGAGCT GGTGGAGGTG GTTGGAAATG GAACCTATGG ACAAGTCTAC 
AAGGGGCGAC ATGTCAAGAC TGGACAACTG GCTGCCATCA AAGTTATGGA TGTCACAGAA 
GATGAGGAGG AGGAAATCAA ACTGGAGATC AATATGCTGA AGAAATATTC CCACCACAGA 
AACATAGCCA CCTATTATGG AGCTTTTATC AAGAAGAGCC CCCCGGGTCA CGATGACCAG 
CTGTGGCTGG TAATGGAGTT CTGTGGTGCC GGTTCGATCA CAGATCTGGT GAAGAACACG 
AAGGGAAACC AGCTGAAGGA AGACTGGATC GCTTACATCT CCAGAGAGAT CCTCAGAGGC 
CTTGCTCACC TACACGCCCA CCACGTGATC CACCGTGACA TTAAAGGCCA GAACGTTCTG 
CTGACAGAGA ACGCTGAAGT TAAACTGGTG GACTTCGGCG TGAGCGCCCA GCTGGATCGC 
ACAGTGGGGC GGAGAAACAC CTTCATCGGG ACTCCATATT GGATGGCGCC TGAGGTCATC 
GCTTGTGATG AGAACCCGGA TGCAACCTAT GATTACAGAA GCGATCTGTG GTCTTGCGGC 
ATCACAGCCA TCGAAATGGC CGAAGGAGCG CCGCCCCTTT GTGACATGCA CCCCATGCGT 
GCGCTCTTCC TCATTCCACG AAACCCCCCA CCGAGGCTCA AATCCAAGAA ATGGTCCAAG 
AAGTTTTTCA GCTTCATCGA GAGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCCCCACA 
GACCAGCTGC TGAAGCACCC GTTCATCCGA GACCAGCCCA ACGAGAGGCA GGTGCGCATC 
CAGCTCAAGG ACCACATCGA CCGGACCAAG AAGAAGAGGG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGATGAGGAG GAGGATCCTC CAGAGCAGGA AGGAGAACCG 
AGCTCCATCA TCAACGCAAC CGAGTCCACC CTGCGACGCG ACTTCATCCG CCTGCAGCAG 
GAGAACAAGG AGCGCTCCGA GGCCATGCGT CACCAGCAGC TCCTCCAGGA GCAGCAGATG 
CGGGAGCAAG AGGAGTACAA GCGGCAGCTC CTGGCAGAGA GGCAGAAGCG CATCGAGCAG 
CAGAAGGAGC AGAGGCGGCG GCTGGAGGAG CAACAACGAT GCGAACGGGA AATGAGGAGG 
CAGCAGGAGC GCGAGCAGCG GCGGCGCGAG CAGGAGGAGA AAAGACGAAT GGAGGAGATG 
GACCGCAGGC GAAAAGAGGA AGAGGAGCGC AGACGCGCCG AAGACGAAAA GAGAAGGAAC 
GATCGGGAGC AGGAGTACAT TAGACGGCAG CTGGAGGAGG AGCAGCGACA CCTGGAGAAG 
CTGCAGGAGC AGCTGCTGCG AGAGCAGGCC ATGCTGCTGG AGTTCAAGTG GCGGGAGCTG 
GACGAGCAGC GCAAAGCCGA GAGACTGCAT AAGCGGCTGC AGCTGGAGCA GGTGTACCTG 
CTGTCCCTGC AGCATGAATC CAAGCAGCAG CACCCTGCTG ACAGGACAGC CCCCTCCTCA 
GAGCCCAGCC ACACTCCCCA GGCTTTGACT CTGCCCCCGG CCTTCTTGCC GACCCCACGG 
GCTCAGGTCA TCGACGCTGT CGTTTCTCTG GCCTCTGAGC CCCCCAGAGT CCCCCAGATT 
ACCCCCTCAG ACAGGACAGT GCCTGAGAAG ACTGACGAGA CCAGTACCAG ACAGATTTCT 
CCCTCCCAAA CCCCCCAGCC CTCTGTAAGC GAAACCCCCA CCGACTCTAA GAGTCCCAAG 
GCAGAAAGTC TGGAGCCTGA CAGGATCTCC GAGCCTGTCA GGCTTCCCAT TCAGCCTATC 
AGAGAGGCGG ATGAGCGCTA CCGTAAAAAC ATCCAGGGCT CCCCGCAGGC CGCCCCTCCT 
CCCAAGCAGC CCCCTCTGCC ACCCCGATCC TCCGAACCCT TCTCCAACGG CGGCTCCTCC 
TCTGAGGCCA TGCACCGGCC CATGGAGCCT CAGGTACCAG TGAGGACAAC ATCCAGATCT 
CCGGTTTTGT CGCGGCGAGA GTCTCCTCTG CCAACGCAGC CCAACAACCA GAGGAACGCC 
GTCGGGAACA TGGAGCAGCG CCCTCTATGG GACAGGGTGG AGAAGCTGCA GTCCCGACCC 
GGCAGCGGCA GCTCCTCCAA CTCCAACTCT CAGGCCAGTC CCGGCGAGCG TTTCAGACCT 
CGCTCTTCCT CTAAATCTGA GGGATCGCCT CTCCAGCGGC CCGAAAACCT CCCCAAAAAA 
CAGGACGAAA GGAACGTGGC CCGGCCGACG CGACCCGCTG ACCTGACGGC GCTGGCTAAG 
GAGCTGCGTG CCGTGGACGA CGTGCGGCCC CCCCACAAGG TCACAGACTA CTCCTCATCG 
AGCGATGACT CGGGCACCAC GGATGAGGAA GACGACGAGG AGGTGGACCA GGAGCCAGGG 
GACGAGTCCA CGTCGGGGGC CGAGGACTCC AGAGCTGGGA GGCTCAGAAA CGGGGAGGCA 
GACTCTGCTA AGACCATGCT GGTGGAGGAC TCTGAGAGTG AGCAGGGCAC CACCCCCTCC 
AAAGACGGGA CCCTGGTCAT CAGACAGAGC ACAGCTGACA TAAAGCGGTT GGTCAATGTC 
TCGTCTTCAT CATCCTCCTC CTCGCCCGGC TCCGGTCGTG GCCCCCCAGA GAAAAACGGC 
CGCATGTACC ACCTCCCCGA CATTATTCAG CAGAGCCATC ATTCCTCCCC CCCCTCCACA 
GCCAGCTTCT CCTCCTTGTC CTCCTCCTCC TCCTCCTCCT TCCCCTCATC ATCTAGCCAG 
GCCAGTCTTG GCGTCTCCTC ACAGATCTCC TTGGAGGAGC TCGTAGCCGT AGAGTCGCAG 
TCAGAGAGCA ACTCCATGTC CAAACACAAG TCTTCCTCTT CGTTCACTCC CTTCATCGAC 
CCACGCCTCC TCCAGATCTC TCCATCAGGC GGCAGCTCCC TCAACAACAT GGCGGTTTTT 
GGACAAGAAG GCCGACCCGC CGACCCACTG CGCTCTGACC CCTCCCGTAA AGGCTCGGTG 
GTCAACGTCA ACCCTGTCAA CACTCGTCCG CCCAGCGACA CTCCCGAGAT CCGCAAGTAC 
AAGAAGAGGT TCAACTCCGA GATTCTGTGC GCGGCGCTCT GGGGGGTGAA CCTGCTGGTG 
GGGACAGAAA GCGGGCTGAT GCTGCTGGAC CGGAGCGGTC AGGGTAAGGT TTACCCGCTG 
ATTAATCGAC GCCGCATCCA GCAGATGGAT GTCCTGGAGG GACTCAACGT CCTGGTAACC 
ATATCAGGTA AAAAGAACAA ACTGCGAGTG TATTACCTGT CGTGGCTCCG AAACAAGATT 
TTGCACAACG ACCCAGAAGT GGAGAAGAAG CAGGGCTGGG TCAACGTGGG CGACCTGGAG 
GGCTGCGTTC ACTACAAAGT CGTAAAGTAC GAGAGGATCA AGTTCCTGGT TCTGGCTTTG 
AAGAACGCTG TGGAGGTGTA CGCCTGGGCG CCCAAACCCT ACCACAAGTT CATGGCCTTC 
AAGTCGTTTG GTGACCTGGT GCACAAACCT CTGCTGGTGG ACCTGACTGT GGAGGAAGGC 
CAAAGGTTAA AGGTCATCTA CGGATCCTGC TCGGGCTTCC ACGCTGTGGA TGTGGACTCG 
GGCGCCGTCT ACGACATCTA CCTCCCCACA CATATCCAGA CCAGCATCCA GTGCCACGCC 
ATCATCATCC TACCCAACAC TGACGGCATC GAGCTGCTGG TCTGTTACGA GGACGAGGGC 
GTCTACGTCA ACACGTACGG GCGCATCACC AAAGACGTGG TGCTGCAGTG GGGAGAAATG 
CCGACTTCCG TGGCCTACAT TAGGTCAAAC CAGATCATGG GCTGGGGCGA AAAGGCCATC 
GAGATCCGCT CGGTGGAGAC GGGACACCTG GACGGCGTCT TCATGCACAA GCGAGCCCAG 
AGACTCAAAT TCCTCTGTGA AAGAAACGAC AAGGTCTTTT TCGCCTCCGT GCGAGCCGGA 
GGCGCCAGTC AAGTATACTT CATGACGCTG GGACGCTCTT CCCTCATGAG CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023821715.1
CDS	220..4356
Translation	MANDSPAKSLVEIDLASLREPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNQLKEDWIAYISREILRGLAHLHAHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIESCLVKNYMQRPPTDQLLKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEDEEEDPPEQEGEPSSIINATESTLRRDFIRLQQENKERSEAMRHQQLLQEQQMREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRCEREMRRQQEREQRRREQEEKRRMEEMDRRRKEEEERRRAEDEKRRNDREQEYIRRQLEEEQRHLEKLQEQLLREQAMLLEFKWRELDEQRKAERLHKRLQLEQVYLLSLQHESKQQHPADRTAPSSEPSHTPQALTLPPAFLPTPRAQVIDAVVSLASEPPRVPQITPSDRTVPEKTDETSTRQISPSQTPQPSVSETPTDSKSPKAESLEPDRISEPVRLPIQPIREADERYRKNIQGSPQAAPPPKQPPLPPRSSEPFSNGGSSSEAMHRPMEPQVPVRTTSRSPVLSRRESPLPTQPNNQRNAVGNMEQRPLWDRVEKLQSRPGSGSSSNSNSQASPGERFRPRSSSKSEGSPLQRPENLPKKQDERNVARPTRPADLTALAKELRAVDDVRPPHKVTDYSSSSDDSGTTDEEDDEEVDQEPGDESTSGAEDSRAGRLRNGEADSAKTMLVEDSESEQGTTPSKDGTLVIRQSTADIKRLVNVSSSSSSSSPGSGRGPPEKNGRMYHLPDIIQQSHHSSPPSTASFSSLSSSSSSSFPSSSSQASLGVSSQISLEELVAVESQSESNSMSKHKSSSSFTPFIDPRLLQISPSGGSSLNNMAVFGQEGRPADPLRSDPSRKGSVVNVNPVNTRPPSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRIQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNKILHNDPEVEKKQGWVNVGDLEGCVHYKVVKYERIKFLVLALKNAVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCSGFHAVDVDSGAVYDIYLPTHIQTSIQCHAIIILPNTDGIELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRAGGASQVYFMTLGRSSLMSW

Target ORF information:

RefSeq Version	XM_023965947.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023965947.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCAGC TAAAAGTCTG GTAGAGATAG ACTTGGCTTC ATTGCGGGAA 
CCAGCTGGGA TATTCGAGCT GGTGGAGGTG GTTGGAAATG GAACCTATGG ACAAGTCTAC 
AAGGGGCGAC ATGTCAAGAC TGGACAACTG GCTGCCATCA AAGTTATGGA TGTCACAGAA 
GATGAGGAGG AGGAAATCAA ACTGGAGATC AATATGCTGA AGAAATATTC CCACCACAGA 
AACATAGCCA CCTATTATGG AGCTTTTATC AAGAAGAGCC CCCCGGGTCA CGATGACCAG 
CTGTGGCTGG TAATGGAGTT CTGTGGTGCC GGTTCGATCA CAGATCTGGT GAAGAACACG 
AAGGGAAACC AGCTGAAGGA AGACTGGATC GCTTACATCT CCAGAGAGAT CCTCAGAGGC 
CTTGCTCACC TACACGCCCA CCACGTGATC CACCGTGACA TTAAAGGCCA GAACGTTCTG 
CTGACAGAGA ACGCTGAAGT TAAACTGGTG GACTTCGGCG TGAGCGCCCA GCTGGATCGC 
ACAGTGGGGC GGAGAAACAC CTTCATCGGG ACTCCATATT GGATGGCGCC TGAGGTCATC 
GCTTGTGATG AGAACCCGGA TGCAACCTAT GATTACAGAA GCGATCTGTG GTCTTGCGGC 
ATCACAGCCA TCGAAATGGC CGAAGGAGCG CCGCCCCTTT GTGACATGCA CCCCATGCGT 
GCGCTCTTCC TCATTCCACG AAACCCCCCA CCGAGGCTCA AATCCAAGAA ATGGTCCAAG 
AAGTTTTTCA GCTTCATCGA GAGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCCCCACA 
GACCAGCTGC TGAAGCACCC GTTCATCCGA GACCAGCCCA ACGAGAGGCA GGTGCGCATC 
CAGCTCAAGG ACCACATCGA CCGGACCAAG AAGAAGAGGG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGATGAGGAG GAGGATCCTC CAGAGCAGGA AGGAGAACCG 
AGCTCCATCA TCAACGCAAC CGAGTCCACC CTGCGACGCG ACTTCATCCG CCTGCAGCAG 
GAGAACAAGG AGCGCTCCGA GGCCATGCGT CACCAGCAGC TCCTCCAGGA GCAGCAGATG 
CGGGAGCAAG AGGAGTACAA GCGGCAGCTC CTGGCAGAGA GGCAGAAGCG CATCGAGCAG 
CAGAAGGAGC AGAGGCGGCG GCTGGAGGAG CAACAACGAT GCGAACGGGA AATGAGGAGG 
CAGCAGGAGC GCGAGCAGCG GCGGCGCGAG CAGGAGGAGA AAAGACGAAT GGAGGAGATG 
GACCGCAGGC GAAAAGAGGA AGAGGAGCGC AGACGCGCCG AAGACGAAAA GAGAAGGAAC 
GATCGGGAGC AGGAGTACAT TAGACGGCAG CTGGAGGAGG AGCAGCGACA CCTGGAGAAG 
CTGCAGGAGC AGCTGCTGCG AGAGCAGGCC ATGCTGCTGG AGTTCAAGTG GCGGGAGCTG 
GACGAGCAGC GCAAAGCCGA GAGACTGCAT AAGCGGCTGC AGCTGGAGCA GGTGTACCTG 
CTGTCCCTGC AGCATGAATC CAAGCAGCAG CACCCTGCTG ACAGGACAGC CCCCTCCTCA 
GAGCCCAGCC ACACTCCCCA GGCTTTGACT CTGCCCCCGG CCTTCTTGCC GACCCCACGG 
GCTCAGGTCA TCGACGCTGT CGTTTCTCTG GCCTCTGAGC CCCCCAGAGT CCCCCAGATT 
ACCCCCTCAG ACAGGACAGT GCCTGAGAAG ACTGACGAGA CCAGTACCAG ACAGATTTCT 
CCCTCCCAAA CCCCCCAGCC CTCTGTAAGC GAAACCCCCA CCGACTCTAA GAGTCCCAAG 
GCAGAAAGTC TGGAGCCTGA CAGGATCTCC GAGCCTGTCA GGCTTCCCAT TCAGCCTATC 
AGAGAGGCGG ATGAGCGCTA CCGTAAAAAC ATCCAGGGCT CCCCGCAGGC CGCCCCTCCT 
CCCAAGCAGC CCCCTCTGCC ACCCCGATCC TCCGAACCCT TCTCCAACGG CGGCTCCTCC 
TCTGAGGCCA TGCACCGGCC CATGGAGCCT CAGGTACCAG TGAGGACAAC ATCCAGATCT 
CCGGTTTTGT CGCGGCGAGA GTCTCCTCTG CCAACGCAGC CCAACAACCA GAGGAACGCC 
GTCGGGAACA TGGAGCAGCG CCCTCTATGG GACAGGGTGG AGAAGCTGCA GTCCCGACCC 
GGCAGCGGCA GCTCCTCCAA CTCCAACTCT CAGGCCAGTC CCGGCGAGCG TTTCAGACCT 
CGCTCTTCCT CTAAATCTGA GGGATCGCCT CTCCAGCGGC CCGAAAACCT CCCCAAAAAA 
CAGGACGAAA GGAACGTGGC CCGGCCGACG CGACCCGCTG ACCTGACGGC GCTGGCTAAG 
GAGCTGCGTG CCGTGGACGA CGTGCGGCCC CCCCACAAGG TCACAGACTA CTCCTCATCG 
AGCGATGACT CGGGCACCAC GGATGAGGAA GACGACGAGG AGGTGGACCA GGAGCCAGGG 
GACGAGTCCA CGTCGGGGGC CGAGGACTCC AGAGCTGGGA GGCTCAGAAA CGGGGAGGCA 
GACTCTGCTA AGACCATGCT GGTGGAGGAC TCTGAGAGTG AGCAGGGCAC CACCCCCTCC 
AAAGACGGGA CCCTGGTCAT CAGACAGAGC ACAGCTGACA TAAAGCGGTT GGTCAATGTC 
TCGTCTTCAT CATCCTCCTC CTCGCCCGGC TCCGGTCGTG GCCCCCCAGA GAAAAACGGC 
CGCATGTACC ACCTCCCCGA CATTATTCAG CAGAGCCATC ATTCCTCCCC CCCCTCCACA 
GCCAGCTTCT CCTCCTTGTC CTCCTCCTCC TCCTCCTCCT TCCCCTCATC ATCTAGCCAG 
GCCAGTCTTG GCGTCTCCTC ACAGATCTCC TTGGAGGAGC TCGTAGCCGT AGAGTCGCAG 
TCAGAGAGCA ACTCCATGTC CAAACACAAG TCTTCCTCTT CGTTCACTCC CTTCATCGAC 
CCACGCCTCC TCCAGATCTC TCCATCAGGC GGCAGCTCCC TCAACAACAT GGCGGTTTTT 
GGACAAGAAG GCCGACCCGC CGACCCACTG CGCTCTGACC CCTCCCGTAA AGGCTCGGTG 
GTCAACGTCA ACCCTGTCAA CACTCGTCCG CCCAGCGACA CTCCCGAGAT CCGCAAGTAC 
AAGAAGAGGT TCAACTCCGA GATTCTGTGC GCGGCGCTCT GGGGGGTGAA CCTGCTGGTG 
GGGACAGAAA GCGGGCTGAT GCTGCTGGAC CGGAGCGGTC AGGGTAAGGT TTACCCGCTG 
ATTAATCGAC GCCGCATCCA GCAGATGGAT GTCCTGGAGG GACTCAACGT CCTGGTAACC 
ATATCAGGTA AAAAGAACAA ACTGCGAGTG TATTACCTGT CGTGGCTCCG AAACAAGATT 
TTGCACAACG ACCCAGAAGT GGAGAAGAAG CAGGGCTGGG TCAACGTGGG CGACCTGGAG 
GGCTGCGTTC ACTACAAAGT CGTAAAGTAC GAGAGGATCA AGTTCCTGGT TCTGGCTTTG 
AAGAACGCTG TGGAGGTGTA CGCCTGGGCG CCCAAACCCT ACCACAAGTT CATGGCCTTC 
AAGTCGTTTG GTGACCTGGT GCACAAACCT CTGCTGGTGG ACCTGACTGT GGAGGAAGGC 
CAAAGGTTAA AGGTCATCTA CGGATCCTGC TCGGGCTTCC ACGCTGTGGA TGTGGACTCG 
GGCGCCGTCT ACGACATCTA CCTCCCCACA CATATCCAGA CCAGCATCCA GTGCCACGCC 
ATCATCATCC TACCCAACAC TGACGGCATC GAGCTGCTGG TCTGTTACGA GGACGAGGGC 
GTCTACGTCA ACACGTACGG GCGCATCACC AAAGACGTGG TGCTGCAGTG GGGAGAAATG 
CCGACTTCCG TGGCCTACAT TAGGTCAAAC CAGATCATGG GCTGGGGCGA AAAGGCCATC 
GAGATCCGCT CGGTGGAGAC GGGACACCTG GACGGCGTCT TCATGCACAA GCGAGCCCAG 
AGACTCAAAT TCCTCTGTGA AAGAAACGAC AAGGTCTTTT TCGCCTCCGT GCGAGCCGGA 
GGCGCCAGTC AAGTATACTT CATGACGCTG GGACGCTCTT CCCTCATGAG CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101174648 ( XM_023965951.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023821719.1 Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OJa112418
Clone ID Related Accession (Same CDS sequence)	XM_023965951.1
Accession Version	XM_023965951.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	traf2 and NCK-interacting protein kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCAGC TAAAAGTCTG GTAGAGATAG ACTTGGCTTC ATTGCGGGAA 
CCAGCTGGGA TATTCGAGCT GGTGGAGGTG GTTGGAAATG GAACCTATGG ACAAGTCTAC 
AAGGGGCGAC ATGTCAAGAC TGGACAACTG GCTGCCATCA AAGTTATGGA TGTCACAGAA 
GATGAGGAGG AGGAAATCAA ACTGGAGATC AATATGCTGA AGAAATATTC CCACCACAGA 
AACATAGCCA CCTATTATGG AGCTTTTATC AAGAAGAGCC CCCCGGGTCA CGATGACCAG 
CTGTGGCTGG TAATGGAGTT CTGTGGTGCC GGTTCGATCA CAGATCTGGT GAAGAACACG 
AAGGGAAACC AGCTGAAGGA AGACTGGATC GCTTACATCT CCAGAGAGAT CCTCAGAGGC 
CTTGCTCACC TACACGCCCA CCACGTGATC CACCGTGACA TTAAAGGCCA GAACGTTCTG 
CTGACAGAGA ACGCTGAAGT TAAACTGGTG GACTTCGGCG TGAGCGCCCA GCTGGATCGC 
ACAGTGGGGC GGAGAAACAC CTTCATCGGG ACTCCATATT GGATGGCGCC TGAGGTCATC 
GCTTGTGATG AGAACCCGGA TGCAACCTAT GATTACAGAA GCGATCTGTG GTCTTGCGGC 
ATCACAGCCA TCGAAATGGC CGAAGGAGCG CCGCCCCTTT GTGACATGCA CCCCATGCGT 
GCGCTCTTCC TCATTCCACG AAACCCCCCA CCGAGGCTCA AATCCAAGAA ATGGTCCAAG 
AAGTTTTTCA GCTTCATCGA GAGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCCCCACA 
GACCAGCTGC TGAAGCACCC GTTCATCCGA GACCAGCCCA ACGAGAGGCA GGTGCGCATC 
CAGCTCAAGG ACCACATCGA CCGGACCAAG AAGAAGAGGG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGATGAGGAG GAGGATCCTC CAGAGCAGGA AGGAGAACCG 
AGCTCCATCA TCAACGCAAC CGAGTCCACC CTGCGACGCG ACTTCATCCG CCTGCAGCAG 
GAGAACAAGG AGCGCTCCGA GGCCATGCGT CACCAGCAGC TCCTCCAGGA GCAGCAGATG 
CGGGAGCAAG AGGAGTACAA GCGGCAGCTC CTGGCAGAGA GGCAGAAGCG CATCGAGCAG 
CAGAAGGAGC AGAGGCGGCG GCTGGAGGAG CAACAACGAT GCGAACGGGA AATGAGGAGG 
CAGCAGGAGC GCGAGCAGCG GCGGCGCGAG CAGGAGGAGA AAAGACGAAT GGAGGAGATG 
GACCGCAGGC GAAAAGAGGA AGAGGAGCGC AGACGCGCCG AAGACGAAAA GAGAAGGAAC 
GATCGGGAGC AGGAGTACAT TAGACGGCAG CTGGAGGAGG AGCAGCGACA CCTGGAGAAG 
CTGCAGGAGC AGCTGCTGCG AGAGCAGGCC ATGCTGCTGG CGGATGAGCG CTACCGTAAA 
AACATCCAGG GCTCCCCGCA GGCCGCCCCT CCTCCCAAGC AGCCCCCTCT GCCACCCCGA 
TCCTCCGAAC CCTTCTCCAA CGGCGGCTCC TCCTCTGAGG CCATGCACCG GCCCATGGAG 
CCTCAGGTCC AGTGGTCACA CCTGGCCGCT CTTAAGAGCA GCAACAGCGC CACCCCCTCT 
CCTCCTCCGC CTGTTGTCGC TCGCTCCCAG TCCTTCAGCG AATCCGGTGG CGTGGCGTCT 
AACTTTGCAC AACTCCACCT GCGCTCCCAG GACCCCCACC ATCAACACCA CCACCACCCA 
TCGCCTGCAT TCACTGACCC CCACCCCCAA CCTCCCCTCC ACCACCCCCA GGCTCAGAAT 
AGGCTGGAAC ACCAGTCCAG CAGTGAAGAG GTTCCTCCAA AGGTACCAGT GAGGACAACA 
TCCAGATCTC CGGTTTTGTC GCGGCGAGAG TCTCCTCTGC CAACGCAGCC CAACAACCAG 
AGGAACGCCG TCGGGAACAT GGAGCAGCGC CCTCTATGGG ACAGGGTGGA GAAGCTGCAG 
TCCCGACCCG GCAGCGGCAG CTCCTCCAAC TCCAACTCTC AGGCCAGTCC CGGCGAGCGT 
TTCAGACCTC GCTGTGAGTC CCCTGCTTCC TCTAAATCTG AGGGATCGCC TCTCCAGCGG 
CCCGAAAACC TCCCCAAAAA ACAGGACGAA AGGAACGTGG CCCGGCCGAC GCGACCCGCT 
GACCTGACGG CGCTGGCTAA GGAGCTGCGT GCCGTGGACG ACGTGCGGCC CCCCCACAAG 
GTCACAGACT ACTCCTCATC GAGCGATGAC TCGGGCACCA CGGATGAGGA AGACGACGAG 
GAGGTGGACC AGGAGCCAGG GGACGAGTCC ACGTCGGGGG CCGAGGACTC CAGAGCTGGG 
AGGCTCAGAA ACGGGGAGGC AGACTCTGCT AAGACCATGC TGGTGGAGGA CTCTGAGAGT 
GAGCAGGGCA CCACCCCCTC CAAAGACGGG ACCCTGGTCA TCAGACAGAG CACAGCTGAC 
ATAAAGCGGT TGGTCAATGT CTCGTCTTCA TCATCCTCCT CCTCGCCCGG CTCCGGTCGT 
GGCCCCCCAG AGAAAAACGG CCGCATGTAC CACCTCCCCG ACATTATTCA GCAGAGCCAT 
CATTCCTCCC CCCCCTCCAC AGCCAGCTTC TCCTCCTTGT CCTCCTCCTC CTCCTCCTCC 
TTCCCCTCAT CATCTAGCCA GGCCAGTCTT GGCGTCTCCT CACAGATCTC CTTGGAGGAG 
CTCGTAGCCG TAGAGTCGCA GTCAGAGAGC AACTCCATGT CCAAACACAA GTCTTCCTCT 
TCGTTCACTC CCTTCATCGA CCCACGCCTC CTCCAGATCT CTCCATCAGG CGGCAGCTCC 
CTCAACAACA TGGCGGTTTT TGGACAAGAA GGCCGACCCG CCGACCCACT GCGCTCTGAC 
CCCTCCCGTA AAGGCTCGGT GGTCAACGTC AACCCTGTCA ACACTCGTCC GCCCAGCGAC 
ACTCCCGAGA TCCGCAAGTA CAAGAAGAGG TTCAACTCCG AGATTCTGTG CGCGGCGCTC 
TGGGGGGTGA ACCTGCTGGT GGGGACAGAA AGCGGGCTGA TGCTGCTGGA CCGGAGCGGT 
CAGGGTAAGG TTTACCCGCT GATTAATCGA CGCCGCATCC AGCAGATGGA TGTCCTGGAG 
GGACTCAACG TCCTGGTAAC CATATCAGGT AAAAAGAACA AACTGCGAGT GTATTACCTG 
TCGTGGCTCC GAAACAAGAT TTTGCACAAC GACCCAGAAG TGGAGAAGAA GCAGGGCTGG 
GTCAACGTGG GCGACCTGGA GGGCTGCGTT CACTACAAAG TCGTAAAGTA CGAGAGGATC 
AAGTTCCTGG TTCTGGCTTT GAAGAACGCT GTGGAGGTGT ACGCCTGGGC GCCCAAACCC 
TACCACAAGT TCATGGCCTT CAAGTCGTTT GGTGACCTGG TGCACAAACC TCTGCTGGTG 
GACCTGACTG TGGAGGAAGG CCAAAGGTTA AAGGTCATCT ACGGATCCTG CTCGGGCTTC 
CACGCTGTGG ATGTGGACTC GGGCGCCGTC TACGACATCT ACCTCCCCAC ACATATCCAG 
ACCAGCATCC AGTGCCACGC CATCATCATC CTACCCAACA CTGACGGCAT CGAGCTGCTG 
GTCTGTTACG AGGACGAGGG CGTCTACGTC AACACGTACG GGCGCATCAC CAAAGACGTG 
GTGCTGCAGT GGGGAGAAAT GCCGACTTCC GTGGCCTACA TTAGGTCAAA CCAGATCATG 
GGCTGGGGCG AAAAGGCCAT CGAGATCCGC TCGGTGGAGA CGGGACACCT GGACGGCGTC 
TTCATGCACA AGCGAGCCCA GAGACTCAAA TTCCTCTGTG AAAGAAACGA CAAGGTCTTT 
TTCGCCTCCG TGCGAGCCGG AGGCGCCAGT CAAGTATACT TCATGACGCT GGGACGCTCT 
TCCCTCATGA GCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023821719.1
CDS	219..4196
Translation	MANDSPAKSLVEIDLASLREPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNQLKEDWIAYISREILRGLAHLHAHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIESCLVKNYMQRPPTDQLLKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEDEEEDPPEQEGEPSSIINATESTLRRDFIRLQQENKERSEAMRHQQLLQEQQMREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRCEREMRRQQEREQRRREQEEKRRMEEMDRRRKEEEERRRAEDEKRRNDREQEYIRRQLEEEQRHLEKLQEQLLREQAMLLADERYRKNIQGSPQAAPPPKQPPLPPRSSEPFSNGGSSSEAMHRPMEPQVQWSHLAALKSSNSATPSPPPPVVARSQSFSESGGVASNFAQLHLRSQDPHHQHHHHPSPAFTDPHPQPPLHHPQAQNRLEHQSSSEEVPPKVPVRTTSRSPVLSRRESPLPTQPNNQRNAVGNMEQRPLWDRVEKLQSRPGSGSSSNSNSQASPGERFRPRCESPASSKSEGSPLQRPENLPKKQDERNVARPTRPADLTALAKELRAVDDVRPPHKVTDYSSSSDDSGTTDEEDDEEVDQEPGDESTSGAEDSRAGRLRNGEADSAKTMLVEDSESEQGTTPSKDGTLVIRQSTADIKRLVNVSSSSSSSSPGSGRGPPEKNGRMYHLPDIIQQSHHSSPPSTASFSSLSSSSSSSFPSSSSQASLGVSSQISLEELVAVESQSESNSMSKHKSSSSFTPFIDPRLLQISPSGGSSLNNMAVFGQEGRPADPLRSDPSRKGSVVNVNPVNTRPPSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRIQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNKILHNDPEVEKKQGWVNVGDLEGCVHYKVVKYERIKFLVLALKNAVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCSGFHAVDVDSGAVYDIYLPTHIQTSIQCHAIIILPNTDGIELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRAGGASQVYFMTLGRSSLMSW

Target ORF information:

RefSeq Version	XM_023965951.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023965951.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCAGC TAAAAGTCTG GTAGAGATAG ACTTGGCTTC ATTGCGGGAA 
CCAGCTGGGA TATTCGAGCT GGTGGAGGTG GTTGGAAATG GAACCTATGG ACAAGTCTAC 
AAGGGGCGAC ATGTCAAGAC TGGACAACTG GCTGCCATCA AAGTTATGGA TGTCACAGAA 
GATGAGGAGG AGGAAATCAA ACTGGAGATC AATATGCTGA AGAAATATTC CCACCACAGA 
AACATAGCCA CCTATTATGG AGCTTTTATC AAGAAGAGCC CCCCGGGTCA CGATGACCAG 
CTGTGGCTGG TAATGGAGTT CTGTGGTGCC GGTTCGATCA CAGATCTGGT GAAGAACACG 
AAGGGAAACC AGCTGAAGGA AGACTGGATC GCTTACATCT CCAGAGAGAT CCTCAGAGGC 
CTTGCTCACC TACACGCCCA CCACGTGATC CACCGTGACA TTAAAGGCCA GAACGTTCTG 
CTGACAGAGA ACGCTGAAGT TAAACTGGTG GACTTCGGCG TGAGCGCCCA GCTGGATCGC 
ACAGTGGGGC GGAGAAACAC CTTCATCGGG ACTCCATATT GGATGGCGCC TGAGGTCATC 
GCTTGTGATG AGAACCCGGA TGCAACCTAT GATTACAGAA GCGATCTGTG GTCTTGCGGC 
ATCACAGCCA TCGAAATGGC CGAAGGAGCG CCGCCCCTTT GTGACATGCA CCCCATGCGT 
GCGCTCTTCC TCATTCCACG AAACCCCCCA CCGAGGCTCA AATCCAAGAA ATGGTCCAAG 
AAGTTTTTCA GCTTCATCGA GAGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCCCCACA 
GACCAGCTGC TGAAGCACCC GTTCATCCGA GACCAGCCCA ACGAGAGGCA GGTGCGCATC 
CAGCTCAAGG ACCACATCGA CCGGACCAAG AAGAAGAGGG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGATGAGGAG GAGGATCCTC CAGAGCAGGA AGGAGAACCG 
AGCTCCATCA TCAACGCAAC CGAGTCCACC CTGCGACGCG ACTTCATCCG CCTGCAGCAG 
GAGAACAAGG AGCGCTCCGA GGCCATGCGT CACCAGCAGC TCCTCCAGGA GCAGCAGATG 
CGGGAGCAAG AGGAGTACAA GCGGCAGCTC CTGGCAGAGA GGCAGAAGCG CATCGAGCAG 
CAGAAGGAGC AGAGGCGGCG GCTGGAGGAG CAACAACGAT GCGAACGGGA AATGAGGAGG 
CAGCAGGAGC GCGAGCAGCG GCGGCGCGAG CAGGAGGAGA AAAGACGAAT GGAGGAGATG 
GACCGCAGGC GAAAAGAGGA AGAGGAGCGC AGACGCGCCG AAGACGAAAA GAGAAGGAAC 
GATCGGGAGC AGGAGTACAT TAGACGGCAG CTGGAGGAGG AGCAGCGACA CCTGGAGAAG 
CTGCAGGAGC AGCTGCTGCG AGAGCAGGCC ATGCTGCTGG CGGATGAGCG CTACCGTAAA 
AACATCCAGG GCTCCCCGCA GGCCGCCCCT CCTCCCAAGC AGCCCCCTCT GCCACCCCGA 
TCCTCCGAAC CCTTCTCCAA CGGCGGCTCC TCCTCTGAGG CCATGCACCG GCCCATGGAG 
CCTCAGGTCC AGTGGTCACA CCTGGCCGCT CTTAAGAGCA GCAACAGCGC CACCCCCTCT 
CCTCCTCCGC CTGTTGTCGC TCGCTCCCAG TCCTTCAGCG AATCCGGTGG CGTGGCGTCT 
AACTTTGCAC AACTCCACCT GCGCTCCCAG GACCCCCACC ATCAACACCA CCACCACCCA 
TCGCCTGCAT TCACTGACCC CCACCCCCAA CCTCCCCTCC ACCACCCCCA GGCTCAGAAT 
AGGCTGGAAC ACCAGTCCAG CAGTGAAGAG GTTCCTCCAA AGGTACCAGT GAGGACAACA 
TCCAGATCTC CGGTTTTGTC GCGGCGAGAG TCTCCTCTGC CAACGCAGCC CAACAACCAG 
AGGAACGCCG TCGGGAACAT GGAGCAGCGC CCTCTATGGG ACAGGGTGGA GAAGCTGCAG 
TCCCGACCCG GCAGCGGCAG CTCCTCCAAC TCCAACTCTC AGGCCAGTCC CGGCGAGCGT 
TTCAGACCTC GCTGTGAGTC CCCTGCTTCC TCTAAATCTG AGGGATCGCC TCTCCAGCGG 
CCCGAAAACC TCCCCAAAAA ACAGGACGAA AGGAACGTGG CCCGGCCGAC GCGACCCGCT 
GACCTGACGG CGCTGGCTAA GGAGCTGCGT GCCGTGGACG ACGTGCGGCC CCCCCACAAG 
GTCACAGACT ACTCCTCATC GAGCGATGAC TCGGGCACCA CGGATGAGGA AGACGACGAG 
GAGGTGGACC AGGAGCCAGG GGACGAGTCC ACGTCGGGGG CCGAGGACTC CAGAGCTGGG 
AGGCTCAGAA ACGGGGAGGC AGACTCTGCT AAGACCATGC TGGTGGAGGA CTCTGAGAGT 
GAGCAGGGCA CCACCCCCTC CAAAGACGGG ACCCTGGTCA TCAGACAGAG CACAGCTGAC 
ATAAAGCGGT TGGTCAATGT CTCGTCTTCA TCATCCTCCT CCTCGCCCGG CTCCGGTCGT 
GGCCCCCCAG AGAAAAACGG CCGCATGTAC CACCTCCCCG ACATTATTCA GCAGAGCCAT 
CATTCCTCCC CCCCCTCCAC AGCCAGCTTC TCCTCCTTGT CCTCCTCCTC CTCCTCCTCC 
TTCCCCTCAT CATCTAGCCA GGCCAGTCTT GGCGTCTCCT CACAGATCTC CTTGGAGGAG 
CTCGTAGCCG TAGAGTCGCA GTCAGAGAGC AACTCCATGT CCAAACACAA GTCTTCCTCT 
TCGTTCACTC CCTTCATCGA CCCACGCCTC CTCCAGATCT CTCCATCAGG CGGCAGCTCC 
CTCAACAACA TGGCGGTTTT TGGACAAGAA GGCCGACCCG CCGACCCACT GCGCTCTGAC 
CCCTCCCGTA AAGGCTCGGT GGTCAACGTC AACCCTGTCA ACACTCGTCC GCCCAGCGAC 
ACTCCCGAGA TCCGCAAGTA CAAGAAGAGG TTCAACTCCG AGATTCTGTG CGCGGCGCTC 
TGGGGGGTGA ACCTGCTGGT GGGGACAGAA AGCGGGCTGA TGCTGCTGGA CCGGAGCGGT 
CAGGGTAAGG TTTACCCGCT GATTAATCGA CGCCGCATCC AGCAGATGGA TGTCCTGGAG 
GGACTCAACG TCCTGGTAAC CATATCAGGT AAAAAGAACA AACTGCGAGT GTATTACCTG 
TCGTGGCTCC GAAACAAGAT TTTGCACAAC GACCCAGAAG TGGAGAAGAA GCAGGGCTGG 
GTCAACGTGG GCGACCTGGA GGGCTGCGTT CACTACAAAG TCGTAAAGTA CGAGAGGATC 
AAGTTCCTGG TTCTGGCTTT GAAGAACGCT GTGGAGGTGT ACGCCTGGGC GCCCAAACCC 
TACCACAAGT TCATGGCCTT CAAGTCGTTT GGTGACCTGG TGCACAAACC TCTGCTGGTG 
GACCTGACTG TGGAGGAAGG CCAAAGGTTA AAGGTCATCT ACGGATCCTG CTCGGGCTTC 
CACGCTGTGG ATGTGGACTC GGGCGCCGTC TACGACATCT ACCTCCCCAC ACATATCCAG 
ACCAGCATCC AGTGCCACGC CATCATCATC CTACCCAACA CTGACGGCAT CGAGCTGCTG 
GTCTGTTACG AGGACGAGGG CGTCTACGTC AACACGTACG GGCGCATCAC CAAAGACGTG 
GTGCTGCAGT GGGGAGAAAT GCCGACTTCC GTGGCCTACA TTAGGTCAAA CCAGATCATG 
GGCTGGGGCG AAAAGGCCAT CGAGATCCGC TCGGTGGAGA CGGGACACCT GGACGGCGTC 
TTCATGCACA AGCGAGCCCA GAGACTCAAA TTCCTCTGTG AAAGAAACGA CAAGGTCTTT 
TTCGCCTCCG TGCGAGCCGG AGGCGCCAGT CAAGTATACT TCATGACGCT GGGACGCTCT 
TCCCTCATGA GCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101174648 ( XM_023965953.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023821721.1 Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X7, mRNA.

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CloneID	OJa112419
Clone ID Related Accession (Same CDS sequence)	XM_023965953.1
Accession Version	XM_023965953.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCAGC TAAAAGTCTG GTAGAGATAG ACTTGGCTTC ATTGCGGGAA 
CCAGCTGGGA TATTCGAGCT GGTGGAGGTG GTTGGAAATG GAACCTATGG ACAAGTCTAC 
AAGGGGCGAC ATGTCAAGAC TGGACAACTG GCTGCCATCA AAGTTATGGA TGTCACAGAA 
GATGAGGAGG AGGAAATCAA ACTGGAGATC AATATGCTGA AGAAATATTC CCACCACAGA 
AACATAGCCA CCTATTATGG AGCTTTTATC AAGAAGAGCC CCCCGGGTCA CGATGACCAG 
CTGTGGCTGG TAATGGAGTT CTGTGGTGCC GGTTCGATCA CAGATCTGGT GAAGAACACG 
AAGGGAAACC AGCTGAAGGA AGACTGGATC GCTTACATCT CCAGAGAGAT CCTCAGAGGC 
CTTGCTCACC TACACGCCCA CCACGTGATC CACCGTGACA TTAAAGGCCA GAACGTTCTG 
CTGACAGAGA ACGCTGAAGT TAAACTGGTG GACTTCGGCG TGAGCGCCCA GCTGGATCGC 
ACAGTGGGGC GGAGAAACAC CTTCATCGGG ACTCCATATT GGATGGCGCC TGAGGTCATC 
GCTTGTGATG AGAACCCGGA TGCAACCTAT GATTACAGAA GCGATCTGTG GTCTTGCGGC 
ATCACAGCCA TCGAAATGGC CGAAGGAGCG CCGCCCCTTT GTGACATGCA CCCCATGCGT 
GCGCTCTTCC TCATTCCACG AAACCCCCCA CCGAGGCTCA AATCCAAGAA ATGGTCCAAG 
AAGTTTTTCA GCTTCATCGA GAGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCCCCACA 
GACCAGCTGC TGAAGCACCC GTTCATCCGA GACCAGCCCA ACGAGAGGCA GGTGCGCATC 
CAGCTCAAGG ACCACATCGA CCGGACCAAG AAGAAGAGGG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGATGAGGAG GAGGATCCTC CAGAGCAGGA AGGAGAACCG 
AGCTCCATCA TCAACGCAAC CGAGTCCACC CTGCGACGCG ACTTCATCCG CCTGCAGCAG 
GAGAACAAGG AGCGCTCCGA GGCCATGCGT CACCAGCAGC TCCTCCAGGA GCAGCAGATG 
CGGGAGCAAG AGGAGTACAA GCGGCAGCTC CTGGCAGAGA GGCAGAAGCG CATCGAGCAG 
CAGAAGGAGC AGAGGCGGCG GCTGGAGGAG CAACAACGAT GCGAACGGGA AATGAGGAGG 
CAGCAGGAGC GCGAGCAGCG GCGGCGCGAG CAGGAGGAGA AAAGACGAAT GGAGGAGATG 
GACCGCAGGC GAAAAGAGGA AGAGGAGCGC AGACGCGCCG AAGACGAAAA GAGAAGGAAC 
GATCGGGAGC AGGAGTACAT TAGACGGCAG CTGGAGGAGG AGCAGCGACA CCTGGAGAAG 
CTGCAGGAGC AGCTGCTGCG AGAGCAGGCC ATGCTGCTGG AGTTCAAGTG GCGGGAGCTG 
GACGAGCAGC GCAAAGCCGA GAGACTGCAT AAGCGGCTGC AGCTGGAGCA GGTGTACCTG 
CTGTCCCTGC AGCATGAATC CAAGCAGCAG CACCCTGCTG ACAGGACAGC CCCCTCCTCA 
GAGCCCAGCC ACACTCCCCA GGCTTTGACT CTGCCCCCGG CCTTCTTGCC GACCCCACGG 
GCTCAGGTCA TCGACGCTGT CGTTTCTCTG GCCTCTGAGC CCCCCAGAGT CCCCCAGATT 
ACCCCCTCAG ACAGGACAGT GCCTGAGAAG ACTGACGAGA CCAGTACCAG ACAGATTTCT 
CCCTCCCAAA CCCCCCAGCC CTCTGTAAGC GAAACCCCCA CCGACTCTAA GAGTCCCAAG 
GCAGAAAGTC TGGAGCCTGA CAGGATCTCC GAGCCTGTCA GGCTTCCCAT TCAGCCTATC 
AGAGAGGCGG ATGAGCGCTA CCGTAAAAAC ATCCAGGGCT CCCCGCAGGC CGCCCCTCCT 
CCCAAGCAGC CCCCTCTGCC ACCCCGATCC TCCGAACCCT TCTCCAACGG CGGCTCCTCC 
TCTGAGGCCA TGCACCGGCC CATGGAGCCT CAGGTACCAG TGAGGACAAC ATCCAGATCT 
CCGGTTTTGT CGCGGCGAGA GTCTCCTCTG CCAACGCAGC CCAACAACCA GAGGAACGCC 
GTCGGGAACA TGGAGCAGCG CCCTCTATGG GACAGGGTGG AGAAGCTGCA GTCCCGACCC 
GGCAGCGGCA GCTCCTCCAA CTCCAACTCT CAGGCCAGTC CCGGCGAGCG TTTCAGACCT 
CGCTCTTCCT CTAAATCTGA GGGATCGCCT CTCCAGCGGC CCGAAAACCT CCCCAAAAAA 
CAGGACGAAA GGAACGTGGC CCGGCCGACG CGACCCGCTG ACCTGACGGC GCTGGCTAAG 
GAGCTGCGTG CCGTGGACGA CGTGCGGCCC CCCCACAAGG TCACAGACTA CTCCTCATCG 
AGCGATGACT CGGGCACCAC GGATGAGGAA GACGACGAGG AGGTGGACCA GGAGCCAGGG 
GACGAGTCCA CGTCGGGGGC CGAGGACTCC AGAGCTGGGA GGCTCAGAAA CGGGGAGGCA 
GACTCTGCTA AGACCATGCT GGTGGAGGAC TCTGAGAGTG AGCAGGGCAC CACCCCCTCC 
AAAGACGGGA CCCTGGTCAT CAGACAGTCG CAGTCAGAGA GCAACTCCAT GTCCAAACAC 
AAGTCTTCCT CTTCGTTCAC TCCCTTCATC GACCCACGCC TCCTCCAGAT CTCTCCATCA 
GGCGGCAGCT CCCTCAACAA CATGGCGGTT TTTGGACAAG AAGGCCGACC CGCCGACCCA 
CTGCGCTCTG ACCCCTCCCG TAAAGGCTCG GTGGTCAACG TCAACCCTGT CAACACTCGT 
CCGCCCAGCG ACACTCCCGA GATCCGCAAG TACAAGAAGA GGTTCAACTC CGAGATTCTG 
TGCGCGGCGC TCTGGGGGGT GAACCTGCTG GTGGGGACAG AAAGCGGGCT GATGCTGCTG 
GACCGGAGCG GTCAGGGTAA GGTTTACCCG CTGATTAATC GACGCCGCAT CCAGCAGATG 
GATGTCCTGG AGGGACTCAA CGTCCTGGTA ACCATATCAG GTAAAAAGAA CAAACTGCGA 
GTGTATTACC TGTCGTGGCT CCGAAACAAG ATTTTGCACA ACGACCCAGA AGTGGAGAAG 
AAGCAGGGCT GGGTCAACGT GGGCGACCTG GAGGGCTGCG TTCACTACAA AGTCGTAAAG 
TACGAGAGGA TCAAGTTCCT GGTTCTGGCT TTGAAGAACG CTGTGGAGGT GTACGCCTGG 
GCGCCCAAAC CCTACCACAA GTTCATGGCC TTCAAGTCGT TTGGTGACCT GGTGCACAAA 
CCTCTGCTGG TGGACCTGAC TGTGGAGGAA GGCCAAAGGT TAAAGGTCAT CTACGGATCC 
TGCTCGGGCT TCCACGCTGT GGATGTGGAC TCGGGCGCCG TCTACGACAT CTACCTCCCC 
ACACATATCC AGACCAGCAT CCAGTGCCAC GCCATCATCA TCCTACCCAA CACTGACGGC 
ATCGAGCTGC TGGTCTGTTA CGAGGACGAG GGCGTCTACG TCAACACGTA CGGGCGCATC 
ACCAAAGACG TGGTGCTGCA GTGGGGAGAA ATGCCGACTT CCGTGGCCTA CATTAGGTCA 
AACCAGATCA TGGGCTGGGG CGAAAAGGCC ATCGAGATCC GCTCGGTGGA GACGGGACAC 
CTGGACGGCG TCTTCATGCA CAAGCGAGCC CAGAGACTCA AATTCCTCTG TGAAAGAAAC 
GACAAGGTCT TTTTCGCCTC CGTGCGAGCC GGAGGCGCCA GTCAAGTATA CTTCATGACG 
CTGGGACGCT CTTCCCTCAT GAGCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023821721.1
CDS	220..4089
Translation	MANDSPAKSLVEIDLASLREPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNQLKEDWIAYISREILRGLAHLHAHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIESCLVKNYMQRPPTDQLLKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEDEEEDPPEQEGEPSSIINATESTLRRDFIRLQQENKERSEAMRHQQLLQEQQMREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRCEREMRRQQEREQRRREQEEKRRMEEMDRRRKEEEERRRAEDEKRRNDREQEYIRRQLEEEQRHLEKLQEQLLREQAMLLEFKWRELDEQRKAERLHKRLQLEQVYLLSLQHESKQQHPADRTAPSSEPSHTPQALTLPPAFLPTPRAQVIDAVVSLASEPPRVPQITPSDRTVPEKTDETSTRQISPSQTPQPSVSETPTDSKSPKAESLEPDRISEPVRLPIQPIREADERYRKNIQGSPQAAPPPKQPPLPPRSSEPFSNGGSSSEAMHRPMEPQVPVRTTSRSPVLSRRESPLPTQPNNQRNAVGNMEQRPLWDRVEKLQSRPGSGSSSNSNSQASPGERFRPRSSSKSEGSPLQRPENLPKKQDERNVARPTRPADLTALAKELRAVDDVRPPHKVTDYSSSSDDSGTTDEEDDEEVDQEPGDESTSGAEDSRAGRLRNGEADSAKTMLVEDSESEQGTTPSKDGTLVIRQSQSESNSMSKHKSSSSFTPFIDPRLLQISPSGGSSLNNMAVFGQEGRPADPLRSDPSRKGSVVNVNPVNTRPPSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRIQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNKILHNDPEVEKKQGWVNVGDLEGCVHYKVVKYERIKFLVLALKNAVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCSGFHAVDVDSGAVYDIYLPTHIQTSIQCHAIIILPNTDGIELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRAGGASQVYFMTLGRSSLMSW

Target ORF information:

RefSeq Version	XM_023965953.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4 (LOC101174648), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023965953.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCAGC TAAAAGTCTG GTAGAGATAG ACTTGGCTTC ATTGCGGGAA 
CCAGCTGGGA TATTCGAGCT GGTGGAGGTG GTTGGAAATG GAACCTATGG ACAAGTCTAC 
AAGGGGCGAC ATGTCAAGAC TGGACAACTG GCTGCCATCA AAGTTATGGA TGTCACAGAA 
GATGAGGAGG AGGAAATCAA ACTGGAGATC AATATGCTGA AGAAATATTC CCACCACAGA 
AACATAGCCA CCTATTATGG AGCTTTTATC AAGAAGAGCC CCCCGGGTCA CGATGACCAG 
CTGTGGCTGG TAATGGAGTT CTGTGGTGCC GGTTCGATCA CAGATCTGGT GAAGAACACG 
AAGGGAAACC AGCTGAAGGA AGACTGGATC GCTTACATCT CCAGAGAGAT CCTCAGAGGC 
CTTGCTCACC TACACGCCCA CCACGTGATC CACCGTGACA TTAAAGGCCA GAACGTTCTG 
CTGACAGAGA ACGCTGAAGT TAAACTGGTG GACTTCGGCG TGAGCGCCCA GCTGGATCGC 
ACAGTGGGGC GGAGAAACAC CTTCATCGGG ACTCCATATT GGATGGCGCC TGAGGTCATC 
GCTTGTGATG AGAACCCGGA TGCAACCTAT GATTACAGAA GCGATCTGTG GTCTTGCGGC 
ATCACAGCCA TCGAAATGGC CGAAGGAGCG CCGCCCCTTT GTGACATGCA CCCCATGCGT 
GCGCTCTTCC TCATTCCACG AAACCCCCCA CCGAGGCTCA AATCCAAGAA ATGGTCCAAG 
AAGTTTTTCA GCTTCATCGA GAGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCCCCACA 
GACCAGCTGC TGAAGCACCC GTTCATCCGA GACCAGCCCA ACGAGAGGCA GGTGCGCATC 
CAGCTCAAGG ACCACATCGA CCGGACCAAG AAGAAGAGGG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGATGAGGAG GAGGATCCTC CAGAGCAGGA AGGAGAACCG 
AGCTCCATCA TCAACGCAAC CGAGTCCACC CTGCGACGCG ACTTCATCCG CCTGCAGCAG 
GAGAACAAGG AGCGCTCCGA GGCCATGCGT CACCAGCAGC TCCTCCAGGA GCAGCAGATG 
CGGGAGCAAG AGGAGTACAA GCGGCAGCTC CTGGCAGAGA GGCAGAAGCG CATCGAGCAG 
CAGAAGGAGC AGAGGCGGCG GCTGGAGGAG CAACAACGAT GCGAACGGGA AATGAGGAGG 
CAGCAGGAGC GCGAGCAGCG GCGGCGCGAG CAGGAGGAGA AAAGACGAAT GGAGGAGATG 
GACCGCAGGC GAAAAGAGGA AGAGGAGCGC AGACGCGCCG AAGACGAAAA GAGAAGGAAC 
GATCGGGAGC AGGAGTACAT TAGACGGCAG CTGGAGGAGG AGCAGCGACA CCTGGAGAAG 
CTGCAGGAGC AGCTGCTGCG AGAGCAGGCC ATGCTGCTGG AGTTCAAGTG GCGGGAGCTG 
GACGAGCAGC GCAAAGCCGA GAGACTGCAT AAGCGGCTGC AGCTGGAGCA GGTGTACCTG 
CTGTCCCTGC AGCATGAATC CAAGCAGCAG CACCCTGCTG ACAGGACAGC CCCCTCCTCA 
GAGCCCAGCC ACACTCCCCA GGCTTTGACT CTGCCCCCGG CCTTCTTGCC GACCCCACGG 
GCTCAGGTCA TCGACGCTGT CGTTTCTCTG GCCTCTGAGC CCCCCAGAGT CCCCCAGATT 
ACCCCCTCAG ACAGGACAGT GCCTGAGAAG ACTGACGAGA CCAGTACCAG ACAGATTTCT 
CCCTCCCAAA CCCCCCAGCC CTCTGTAAGC GAAACCCCCA CCGACTCTAA GAGTCCCAAG 
GCAGAAAGTC TGGAGCCTGA CAGGATCTCC GAGCCTGTCA GGCTTCCCAT TCAGCCTATC 
AGAGAGGCGG ATGAGCGCTA CCGTAAAAAC ATCCAGGGCT CCCCGCAGGC CGCCCCTCCT 
CCCAAGCAGC CCCCTCTGCC ACCCCGATCC TCCGAACCCT TCTCCAACGG CGGCTCCTCC 
TCTGAGGCCA TGCACCGGCC CATGGAGCCT CAGGTACCAG TGAGGACAAC ATCCAGATCT 
CCGGTTTTGT CGCGGCGAGA GTCTCCTCTG CCAACGCAGC CCAACAACCA GAGGAACGCC 
GTCGGGAACA TGGAGCAGCG CCCTCTATGG GACAGGGTGG AGAAGCTGCA GTCCCGACCC 
GGCAGCGGCA GCTCCTCCAA CTCCAACTCT CAGGCCAGTC CCGGCGAGCG TTTCAGACCT 
CGCTCTTCCT CTAAATCTGA GGGATCGCCT CTCCAGCGGC CCGAAAACCT CCCCAAAAAA 
CAGGACGAAA GGAACGTGGC CCGGCCGACG CGACCCGCTG ACCTGACGGC GCTGGCTAAG 
GAGCTGCGTG CCGTGGACGA CGTGCGGCCC CCCCACAAGG TCACAGACTA CTCCTCATCG 
AGCGATGACT CGGGCACCAC GGATGAGGAA GACGACGAGG AGGTGGACCA GGAGCCAGGG 
GACGAGTCCA CGTCGGGGGC CGAGGACTCC AGAGCTGGGA GGCTCAGAAA CGGGGAGGCA 
GACTCTGCTA AGACCATGCT GGTGGAGGAC TCTGAGAGTG AGCAGGGCAC CACCCCCTCC 
AAAGACGGGA CCCTGGTCAT CAGACAGTCG CAGTCAGAGA GCAACTCCAT GTCCAAACAC 
AAGTCTTCCT CTTCGTTCAC TCCCTTCATC GACCCACGCC TCCTCCAGAT CTCTCCATCA 
GGCGGCAGCT CCCTCAACAA CATGGCGGTT TTTGGACAAG AAGGCCGACC CGCCGACCCA 
CTGCGCTCTG ACCCCTCCCG TAAAGGCTCG GTGGTCAACG TCAACCCTGT CAACACTCGT 
CCGCCCAGCG ACACTCCCGA GATCCGCAAG TACAAGAAGA GGTTCAACTC CGAGATTCTG 
TGCGCGGCGC TCTGGGGGGT GAACCTGCTG GTGGGGACAG AAAGCGGGCT GATGCTGCTG 
GACCGGAGCG GTCAGGGTAA GGTTTACCCG CTGATTAATC GACGCCGCAT CCAGCAGATG 
GATGTCCTGG AGGGACTCAA CGTCCTGGTA ACCATATCAG GTAAAAAGAA CAAACTGCGA 
GTGTATTACC TGTCGTGGCT CCGAAACAAG ATTTTGCACA ACGACCCAGA AGTGGAGAAG 
AAGCAGGGCT GGGTCAACGT GGGCGACCTG GAGGGCTGCG TTCACTACAA AGTCGTAAAG 
TACGAGAGGA TCAAGTTCCT GGTTCTGGCT TTGAAGAACG CTGTGGAGGT GTACGCCTGG 
GCGCCCAAAC CCTACCACAA GTTCATGGCC TTCAAGTCGT TTGGTGACCT GGTGCACAAA 
CCTCTGCTGG TGGACCTGAC TGTGGAGGAA GGCCAAAGGT TAAAGGTCAT CTACGGATCC 
TGCTCGGGCT TCCACGCTGT GGATGTGGAC TCGGGCGCCG TCTACGACAT CTACCTCCCC 
ACACATATCC AGACCAGCAT CCAGTGCCAC GCCATCATCA TCCTACCCAA CACTGACGGC 
ATCGAGCTGC TGGTCTGTTA CGAGGACGAG GGCGTCTACG TCAACACGTA CGGGCGCATC 
ACCAAAGACG TGGTGCTGCA GTGGGGAGAA ATGCCGACTT CCGTGGCCTA CATTAGGTCA 
AACCAGATCA TGGGCTGGGG CGAAAAGGCC ATCGAGATCC GCTCGGTGGA GACGGGACAC 
CTGGACGGCG TCTTCATGCA CAAGCGAGCC CAGAGACTCA AATTCCTCTG TGAAAGAAAC 
GACAAGGTCT TTTTCGCCTC CGTGCGAGCC GGAGGCGCCA GTCAAGTATA CTTCATGACG 
CTGGGACGCT CTTCCCTCAT GAGCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101174648 ( XM_020709038.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020564697.1 Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4-like (LOC101174648), mRNA.

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CloneID	OJa74677
Clone ID Related Accession (Same CDS sequence)	XM_020709038.1
Accession Version	XM_020709038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	mitogen-activated protein kinase kinase kinase kinase 4-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.1) and transcript sequences (GFIO01001420.1 and GFIO01039237.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 369 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCGAACG ACTCCCCAGC TAAAAGTCTG GTAGAGATAG ACTTGGCTTC ATTGCGGGAA 
CCAGCTGGGA TATTCGAGCT GGTGGAGGTG GTTGGAAATG GAACCTATGG ACAAGTCTAC 
AAGGGGCGAC ATGTCAAGAC TGGACAACTG GCTGCCATCA AAGTTATGGA TGTCACAGAA 
GATGAGGAGG AGGAAATCAA ACTGGAGATC AATATGCTGA AGAAATATTC CCACCACAGA 
AACATAGCCA CCTATTATGG AGCTTTTATC AAGAAGAGCC CCCCGGGTCA CGATGACCAG 
CTGTGGCTGG TAATGGAGTT CTGTGGTGCC GGTTCGATCA CAGATCTGGT GAAGAACACG 
AAGGGAAACC AGCTGAAGGA AGACTGGATC GCTTACATCT CCAGAGAGAT CCTCAGAGGC 
CTTGCTCACC TACACGCCCA CCACGTGATC CACCGTGACA TTAAAGGCCA GAACGTTCTG 
CTGACAGAGA ACGCTGAAGT TAAACTGGTG GACTTCGGCG TGAGCGCCCA GCTGGATCGC 
ACAGTGGGGC GGAGAAACAC CTTCATCGGG ACTCCATATT GGATGGCGCC TGAGGTCATC 
GCTTGTGATG AGAACCCGGA TGCAACCTAT GATTACAGAA GCGATCTGTG GTCTTGCGGC 
ATCACAGCCA TCGAAATGGC CGAAGGAGCG CCGCCCCTTT GTGACATGCA CCCCATGCGT 
GCGCTCTTCC TCATTCCACG AAACCCCCCA CCGAGGCTCA AATCCAAGAA ATGGTCCAAG 
AAGTTTTTCA GCTTCATCGA GAGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCCCCACA 
GACCAGCTGC TGAAGCACCC GTTCATCCGA GACCAGCCCA ACGAGAGGCA GGTGCGCATC 
CAGCTCAAGG ACCACATCGA CCGGACCAAG AAGAAGAGGG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGATGAGGAG GAGGATCCTC CAGAGCAGGA AGGAGAACCG 
AGCTCCATCA TCAACGCAAC CGAGTCCACC CTGCGACGCG ACTTCATCCG CCTGCAGCAG 
GAGAACAAGG AGCGCTCCGA GGCCATGCGT CACCAGCAGC TCCTCCAGGA GCAGCAGATG 
CGGGAGCAAG AGGAGTACAA GCGGCAGCTC CTGGCAGAGA GGCAGAAGCG CATCGAGCAG 
CAGAAGGAGC AGAGGCGGCG GCTGGAGGAG CAACAACGAT GCGAACGGGA AATGAGGAGG 
CAGCAGGAGC GCGAGCAGCG GCGGCGCGAG CAGGAGGAGA AAAGACGAAT GGAGGAGATG 
GACCGCAGGC GAAAAGAGGA AGAGGAGCGC AGACGCGCCG AAGACGAAAA GAGAAGGAAC 
GATCGGGAGC AGGAGTACAT TAGACGGCAG CTGGAGGAGG AGCAGCGACA CCTGGAGAAG 
CTGCAGGAGC AGCTGCTGCG AGAGCAGGCC ATGCTGCTGG AGTTCAAGTG GCGGGAGCTG 
GACGAGCAGC GCAAAGCCGA GAGACTGCAT AAGCGGCTGC AGCTGGAGCA GGTGTACCTG 
CTGTCCCTGC AGCATGAATC CAAGCAGCAG CACCCTGCTG ACAGGACAGC CCCCTCCTCA 
GAGCCCAGCC ACACTCCCCA GGCTTTGACT CTGCCCCCGG CCTTCTTGCC GACCCCACGG 
GCTCAGGTCA TCGACGCTGT CGTTTCTCTG GCCTCTGAGC CCCCCAGAGT CCCCCAGATT 
ACCCCCTCAG ACAGGACAGT GCCTGAGAAG ACTGACGAGA CCAGTACCAG ACAGATTTCT 
CCCTCCCAAA CCCCCCAGCC CTCTGTAAGC GAAACCCCCA CCGACTCTAA GAGTCCCAAG 
GCAGAAAGTC TGGAGCCTGA CAGGATCTCC GAGCCTGTCA GGCTTCCCAT TCAGCCTATC 
AGAGAGGCGG ATGAGCGCTA CCGTAAAAAC ATCCAGGGCT CCCCGCAGGC CGCCCCTCCT 
CCCAAGCAGC CCCCTCTGCC ACCCCGATCC TCCGAACCCT TCTCCAACGG CGGCTCCTCC 
TCTGAGGCCA TGCACCGGCC CATGGAGCCT CAGGTCCAGT GGTCACACCT GGCCGCTCTT 
AAGAGCAGCA ACAGCGCCAC CCCCTCTCCT CCTCCGCCTG TTGTCGCTCG CTCCCAGTCC 
TTCAGCGAAT CCGGTGGCGT GGCGTCTAAC TTTGCACAAC TCCACCTGCG CTCCCAGGAC 
CCCCACCATC AACACCACCA CCACCCATCG CCTGCATTCA CTGACCCCCA CCCCCAACCT 
CCCCTCCACC ACCCCCAGGC TCAGAATAGG CTGGAACACC AGTCCAGCAG TGAAGAGGTT 
CCTCCAAAGG TACCAGTGAG GACAACATCC AGATCTCCGG TTTTGTCGCG GCGAGAGTCT 
CCTCTGCCAA CGCAGCCCAA CAACCAGAGG AACGCCGTCG GGAACATGGA GCAGCGCCCT 
CTATGGGACA GGGTGGAGAA GCTGCAGTCC CGACCCGGCA GCGGCAGCTC CTCCAACTCC 
AACTCTCAGG CCAGTCCCGG CGAGCGTTTC AGACCTCGCT GTGAGTCCCC TGCTTCCTCT 
AAATCTGAGG GATCGCCTCT CCAGCGGCCC GAAAACCTCC CCAAAAAACA GGACGAAAGG 
AACGTGGCCC GGCCGACGCG ACCCGCTGAC CTGACGGCGC TGGCTAAGGA GCTGCGTGCC 
GTGGACGACG TGCGGCCCCC CCACAAGGTC ACAGACTACT CCTCATCGAG CGATGACTCG 
GGCACCACGG ATGAGGAAGA CGACGAGGAG GTGGACCAGG AGCCAGGGGA CGAGTCCACG 
TCGGGGGCCG AGGACTCCAG AGCTGGGAGG CTCAGAAACG GGGAGGCAGA CTCTGCTAAG 
ACCATGCTGG TGGAGGACTC TGAGAGTGAG CAGGGCACCA CCCCCTCCAA AGACGGGACC 
CTGGTCATCA GACAGAGCAC AGCCGACATA AAGCGGTTGG TCAATGTCTC GTCTTCATCA 
TCCTCCTCCT CGCCCGGCTC CGGTCGTGGC CCCCCAGAGA AAAACGGCCG CATGTACCAC 
CTCCCCGACA TTATTCAGCA GAGCCATCAT TCCTCCCCCC CCTCCACAGC CAGCTTCTCC 
TCCTTGTCCT CCTCCTCCTC CTCCTCCTTC CCCTCATCAT CTAGCCAGGC CAGTCTTGGC 
GTCTCCTCAC AGATCTCCTT GGAGGAGCTC GTAGCCGTAG AGTCGCAGTC AGAGAGCAAC 
TCCATGTCCA AACACAAGTC TTCCTCTTCG TTCACTCCCT TCATCGACCC ACGCCTCCTC 
CAGATCTCTC CATCAGGCGG CAGCTCCCTC AACAACATGG CGGTTTTTGG ACAAGAAGGC 
CGACCCGCCG ACCCACTGCG CTCTGACCCC TCCCGTAAAG GCTCGGTGGT CAACGTCAAC 
CCTGTCAACA CTCGTCCGCC CAGCGACACT CCCGAGATCC GCAAGTACAA GAAGAGGTTC 
AACTCCGAGA TTCTGTGCGC GGCGCTCTGG GGGGTGAACC TGCTGGTGGG GACAGAAAGC 
GGGCTGATGC TGCTGGACCG GAGCGGTCAG GGTAAGGTTT ACCCGCTGAT TAATCGACGC 
CGCATCCAGC AGATGGATGT CCTGGAGGGA CTCAACGTCC TGGTAACCAT ATCAGGTAAA 
AAGAACAAAC TGCGAGTGTA TTACCTGTCG TGGCTCCGAA ACAAGATTTT GCACAACGAC 
CCAGAAGTGG AGAAGAAGCA GGGCTGGGTC AACGTGGGCG ACCTGGAGGG CTGCGTTCAC 
TACAAAGTCG TAAAGTACGA GAGGATCAAG TTCCTGGTTC TGGCTTTGAA GAACGCTGTG 
GAGGTGTACG CCTGGGCGCC CAAACCCTAC CACAAGTTCA TGGCCTTCAA GTCGTTTGGT 
GACCTGGTGC ACAAACCTCT GCTGGTGGAC CTGACTGTGG AGGAAGGCCA AAGGTTAAAG 
GTCATCTACG GATCCTGCTC GGGCTTCCAC GCTGTGGATG TGGACTCGGG CGCCGTCTAC 
GACATCTACC TCCCCACACA TATCCAGACC AGCATCCAGT GCCACGCCAT CATCATCCTA 
CCCAACACTG ACGGCATCGA GCTGCTGGTC TGTTACGAGG ACGAGGGCGT CTACGTCAAC 
ACGTACGGGC GCATCACCAA AGACGTGGTG CTGCAGTGGG GAGAAATGCC GACTTCCGTG 
GCCTACATTA GGTCAAACCA GATCATGGGC TGGGGCGAAA AGGCCATCGA GATCCGCTCG 
GTGGAGACGG GACACCTGGA CGGCGTCTTC ATGCACAAGC GAGCCCAGAG ACTCAAATTC 
CTCTGTGAAA GAAACGACAA GGTCTTTTTC GCCTCCGTGC GAGCCGGAGG CGCCAGTCAA 
GTATACTTCA TGACGCTGGG ACGCTCTTCC CTCATGAGCT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020564697.1
CDS	226..4650
Translation	MANDSPAKSLVEIDLASLREPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNQLKEDWIAYISREILRGLAHLHAHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIESCLVKNYMQRPPTDQLLKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEDEEEDPPEQEGEPSSIINATESTLRRDFIRLQQENKERSEAMRHQQLLQEQQMREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRCEREMRRQQEREQRRREQEEKRRMEEMDRRRKEEEERRRAEDEKRRNDREQEYIRRQLEEEQRHLEKLQEQLLREQAMLLEFKWRELDEQRKAERLHKRLQLEQVYLLSLQHESKQQHPADRTAPSSEPSHTPQALTLPPAFLPTPRAQVIDAVVSLASEPPRVPQITPSDRTVPEKTDETSTRQISPSQTPQPSVSETPTDSKSPKAESLEPDRISEPVRLPIQPIREADERYRKNIQGSPQAAPPPKQPPLPPRSSEPFSNGGSSSEAMHRPMEPQVQWSHLAALKSSNSATPSPPPPVVARSQSFSESGGVASNFAQLHLRSQDPHHQHHHHPSPAFTDPHPQPPLHHPQAQNRLEHQSSSEEVPPKVPVRTTSRSPVLSRRESPLPTQPNNQRNAVGNMEQRPLWDRVEKLQSRPGSGSSSNSNSQASPGERFRPRCESPASSKSEGSPLQRPENLPKKQDERNVARPTRPADLTALAKELRAVDDVRPPHKVTDYSSSSDDSGTTDEEDDEEVDQEPGDESTSGAEDSRAGRLRNGEADSAKTMLVEDSESEQGTTPSKDGTLVIRQSTADIKRLVNVSSSSSSSSPGSGRGPPEKNGRMYHLPDIIQQSHHSSPPSTASFSSLSSSSSSSFPSSSSQASLGVSSQISLEELVAVESQSESNSMSKHKSSSSFTPFIDPRLLQISPSGGSSLNNMAVFGQEGRPADPLRSDPSRKGSVVNVNPVNTRPPSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRIQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNKILHNDPEVEKKQGWVNVGDLEGCVHYKVVKYERIKFLVLALKNAVEVYAWAPKPYHKFMAFKSFGDLVHKPLLVDLTVEEGQRLKVIYGSCSGFHAVDVDSGAVYDIYLPTHIQTSIQCHAIIILPNTDGIELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRAGGASQVYFMTLGRSSLMSW

Target ORF information:

RefSeq Version	XM_020709038.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes mitogen-activated protein kinase kinase kinase kinase 4-like (LOC101174648), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020709038.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCAGC TAAAAGTCTG GTAGAGATAG ACTTGGCTTC ATTGCGGGAA 
CCAGCTGGGA TATTCGAGCT GGTGGAGGTG GTTGGAAATG GAACCTATGG ACAAGTCTAC 
AAGGGGCGAC ATGTCAAGAC TGGACAACTG GCTGCCATCA AAGTTATGGA TGTCACAGAA 
GATGAGGAGG AGGAAATCAA ACTGGAGATC AATATGCTGA AGAAATATTC CCACCACAGA 
AACATAGCCA CCTATTATGG AGCTTTTATC AAGAAGAGCC CCCCGGGTCA CGATGACCAG 
CTGTGGCTGG TAATGGAGTT CTGTGGTGCC GGTTCGATCA CAGATCTGGT GAAGAACACG 
AAGGGAAACC AGCTGAAGGA AGACTGGATC GCTTACATCT CCAGAGAGAT CCTCAGAGGC 
CTTGCTCACC TACACGCCCA CCACGTGATC CACCGTGACA TTAAAGGCCA GAACGTTCTG 
CTGACAGAGA ACGCTGAAGT TAAACTGGTG GACTTCGGCG TGAGCGCCCA GCTGGATCGC 
ACAGTGGGGC GGAGAAACAC CTTCATCGGG ACTCCATATT GGATGGCGCC TGAGGTCATC 
GCTTGTGATG AGAACCCGGA TGCAACCTAT GATTACAGAA GCGATCTGTG GTCTTGCGGC 
ATCACAGCCA TCGAAATGGC CGAAGGAGCG CCGCCCCTTT GTGACATGCA CCCCATGCGT 
GCGCTCTTCC TCATTCCACG AAACCCCCCA CCGAGGCTCA AATCCAAGAA ATGGTCCAAG 
AAGTTTTTCA GCTTCATCGA GAGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCCCCACA 
GACCAGCTGC TGAAGCACCC GTTCATCCGA GACCAGCCCA ACGAGAGGCA GGTGCGCATC 
CAGCTCAAGG ACCACATCGA CCGGACCAAG AAGAAGAGGG GCGAGAAAGA TGAGACGGAG 
TACGAGTACA GTGGCAGTGA GGATGAGGAG GAGGATCCTC CAGAGCAGGA AGGAGAACCG 
AGCTCCATCA TCAACGCAAC CGAGTCCACC CTGCGACGCG ACTTCATCCG CCTGCAGCAG 
GAGAACAAGG AGCGCTCCGA GGCCATGCGT CACCAGCAGC TCCTCCAGGA GCAGCAGATG 
CGGGAGCAAG AGGAGTACAA GCGGCAGCTC CTGGCAGAGA GGCAGAAGCG CATCGAGCAG 
CAGAAGGAGC AGAGGCGGCG GCTGGAGGAG CAACAACGAT GCGAACGGGA AATGAGGAGG 
CAGCAGGAGC GCGAGCAGCG GCGGCGCGAG CAGGAGGAGA AAAGACGAAT GGAGGAGATG 
GACCGCAGGC GAAAAGAGGA AGAGGAGCGC AGACGCGCCG AAGACGAAAA GAGAAGGAAC 
GATCGGGAGC AGGAGTACAT TAGACGGCAG CTGGAGGAGG AGCAGCGACA CCTGGAGAAG 
CTGCAGGAGC AGCTGCTGCG AGAGCAGGCC ATGCTGCTGG AGTTCAAGTG GCGGGAGCTG 
GACGAGCAGC GCAAAGCCGA GAGACTGCAT AAGCGGCTGC AGCTGGAGCA GGTGTACCTG 
CTGTCCCTGC AGCATGAATC CAAGCAGCAG CACCCTGCTG ACAGGACAGC CCCCTCCTCA 
GAGCCCAGCC ACACTCCCCA GGCTTTGACT CTGCCCCCGG CCTTCTTGCC GACCCCACGG 
GCTCAGGTCA TCGACGCTGT CGTTTCTCTG GCCTCTGAGC CCCCCAGAGT CCCCCAGATT 
ACCCCCTCAG ACAGGACAGT GCCTGAGAAG ACTGACGAGA CCAGTACCAG ACAGATTTCT 
CCCTCCCAAA CCCCCCAGCC CTCTGTAAGC GAAACCCCCA CCGACTCTAA GAGTCCCAAG 
GCAGAAAGTC TGGAGCCTGA CAGGATCTCC GAGCCTGTCA GGCTTCCCAT TCAGCCTATC 
AGAGAGGCGG ATGAGCGCTA CCGTAAAAAC ATCCAGGGCT CCCCGCAGGC CGCCCCTCCT 
CCCAAGCAGC CCCCTCTGCC ACCCCGATCC TCCGAACCCT TCTCCAACGG CGGCTCCTCC 
TCTGAGGCCA TGCACCGGCC CATGGAGCCT CAGGTCCAGT GGTCACACCT GGCCGCTCTT 
AAGAGCAGCA ACAGCGCCAC CCCCTCTCCT CCTCCGCCTG TTGTCGCTCG CTCCCAGTCC 
TTCAGCGAAT CCGGTGGCGT GGCGTCTAAC TTTGCACAAC TCCACCTGCG CTCCCAGGAC 
CCCCACCATC AACACCACCA CCACCCATCG CCTGCATTCA CTGACCCCCA CCCCCAACCT 
CCCCTCCACC ACCCCCAGGC TCAGAATAGG CTGGAACACC AGTCCAGCAG TGAAGAGGTT 
CCTCCAAAGG TACCAGTGAG GACAACATCC AGATCTCCGG TTTTGTCGCG GCGAGAGTCT 
CCTCTGCCAA CGCAGCCCAA CAACCAGAGG AACGCCGTCG GGAACATGGA GCAGCGCCCT 
CTATGGGACA GGGTGGAGAA GCTGCAGTCC CGACCCGGCA GCGGCAGCTC CTCCAACTCC 
AACTCTCAGG CCAGTCCCGG CGAGCGTTTC AGACCTCGCT GTGAGTCCCC TGCTTCCTCT 
AAATCTGAGG GATCGCCTCT CCAGCGGCCC GAAAACCTCC CCAAAAAACA GGACGAAAGG 
AACGTGGCCC GGCCGACGCG ACCCGCTGAC CTGACGGCGC TGGCTAAGGA GCTGCGTGCC 
GTGGACGACG TGCGGCCCCC CCACAAGGTC ACAGACTACT CCTCATCGAG CGATGACTCG 
GGCACCACGG ATGAGGAAGA CGACGAGGAG GTGGACCAGG AGCCAGGGGA CGAGTCCACG 
TCGGGGGCCG AGGACTCCAG AGCTGGGAGG CTCAGAAACG GGGAGGCAGA CTCTGCTAAG 
ACCATGCTGG TGGAGGACTC TGAGAGTGAG CAGGGCACCA CCCCCTCCAA AGACGGGACC 
CTGGTCATCA GACAGAGCAC AGCCGACATA AAGCGGTTGG TCAATGTCTC GTCTTCATCA 
TCCTCCTCCT CGCCCGGCTC CGGTCGTGGC CCCCCAGAGA AAAACGGCCG CATGTACCAC 
CTCCCCGACA TTATTCAGCA GAGCCATCAT TCCTCCCCCC CCTCCACAGC CAGCTTCTCC 
TCCTTGTCCT CCTCCTCCTC CTCCTCCTTC CCCTCATCAT CTAGCCAGGC CAGTCTTGGC 
GTCTCCTCAC AGATCTCCTT GGAGGAGCTC GTAGCCGTAG AGTCGCAGTC AGAGAGCAAC 
TCCATGTCCA AACACAAGTC TTCCTCTTCG TTCACTCCCT TCATCGACCC ACGCCTCCTC 
CAGATCTCTC CATCAGGCGG CAGCTCCCTC AACAACATGG CGGTTTTTGG ACAAGAAGGC 
CGACCCGCCG ACCCACTGCG CTCTGACCCC TCCCGTAAAG GCTCGGTGGT CAACGTCAAC 
CCTGTCAACA CTCGTCCGCC CAGCGACACT CCCGAGATCC GCAAGTACAA GAAGAGGTTC 
AACTCCGAGA TTCTGTGCGC GGCGCTCTGG GGGGTGAACC TGCTGGTGGG GACAGAAAGC 
GGGCTGATGC TGCTGGACCG GAGCGGTCAG GGTAAGGTTT ACCCGCTGAT TAATCGACGC 
CGCATCCAGC AGATGGATGT CCTGGAGGGA CTCAACGTCC TGGTAACCAT ATCAGGTAAA 
AAGAACAAAC TGCGAGTGTA TTACCTGTCG TGGCTCCGAA ACAAGATTTT GCACAACGAC 
CCAGAAGTGG AGAAGAAGCA GGGCTGGGTC AACGTGGGCG ACCTGGAGGG CTGCGTTCAC 
TACAAAGTCG TAAAGTACGA GAGGATCAAG TTCCTGGTTC TGGCTTTGAA GAACGCTGTG 
GAGGTGTACG CCTGGGCGCC CAAACCCTAC CACAAGTTCA TGGCCTTCAA GTCGTTTGGT 
GACCTGGTGC ACAAACCTCT GCTGGTGGAC CTGACTGTGG AGGAAGGCCA AAGGTTAAAG 
GTCATCTACG GATCCTGCTC GGGCTTCCAC GCTGTGGATG TGGACTCGGG CGCCGTCTAC 
GACATCTACC TCCCCACACA TATCCAGACC AGCATCCAGT GCCACGCCAT CATCATCCTA 
CCCAACACTG ACGGCATCGA GCTGCTGGTC TGTTACGAGG ACGAGGGCGT CTACGTCAAC 
ACGTACGGGC GCATCACCAA AGACGTGGTG CTGCAGTGGG GAGAAATGCC GACTTCCGTG 
GCCTACATTA GGTCAAACCA GATCATGGGC TGGGGCGAAA AGGCCATCGA GATCCGCTCG 
GTGGAGACGG GACACCTGGA CGGCGTCTTC ATGCACAAGC GAGCCCAGAG ACTCAAATTC 
CTCTGTGAAA GAAACGACAA GGTCTTTTTC GCCTCCGTGC GAGCCGGAGG CGCCAGTCAA 
GTATACTTCA TGACGCTGGG ACGCTCTTCC CTCATGAGCT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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LOC101174648 cDNA ORF clone, Oryzias latipes(Japanese medaka)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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