pgap1 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	pgap1
Entrez Gene ID	101174236
Full Name	post-GPI attachment to proteins 1
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004082046.3	XP_004082094.1	GPI inositol-deacylase isoform X1
XM_020713057.1	XP_020568716.1	GPI inositol-deacylase isoform X2
XM_004082046.4	XP_004082094.1	GPI inositol-deacylase

Pathways from BioSystems
Gene Ontology

The following pgap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pgap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa16215	XM_004082046.4 Latest version!	Oryzias latipes post-GPI attachment to proteins 1 (pgap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OJa75336	XM_020713057.1 Latest version!	Oryzias latipes post-GPI attachment to proteins 1 (pgap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OJa16215	XM_004082046.3	Oryzias latipes post-GPI attachment to proteins 1 (pgap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pgap1 ( XM_004082046.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_004082094.1 Oryzias latipes post-GPI attachment to proteins 1 (pgap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OJa16215
Clone ID Related Accession (Same CDS sequence)	XM_004082046.3 , XM_004082046.4
Accession Version	XM_004082046.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	GPI inositol-deacylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019879.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_004082046.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTGC CCGCCGTGGC CTTTTACGCG CTGGCCGCGG GGCTCCTGGC GGTGGGCCTG 
CGGGAGGTCC TGACGGGCTT CGAGGAGAAC CGCTGCAGCA TGACCTACAT GTTCGAATAC 
CCGGAGTACC GCCGGGTGGC GCTGCCTCGC CGCGTGGCCA GAATGTACCC GGCGTACGGG 
CTTTACCTGT ACGGAGAGGG CCTGTATGCG CAGGAGACCC AGGTGCTCAA ACTCACCGGC 
GCACCTGTTC TCTTCTTACC TGGAAATGCA GGAAGTTACA AACAAGCTCG CTCGCTGGGA 
TCTGTGGCCT TGAGGAAAGC GGAGAACGTG GACGGGGGGC TCCACCTGAA CATGTTCACT 
GTGGACTTCA ACGAGGAGCT GGTGGCTCTG TACGGTGGGA GTTTGTTCAG ACAAACCCAC 
TTCCTGCACG AGAGCATCAA GGCCATCCTG CGGCTCTATA AGCACCTGAA GACCCCCCCT 
CAGAGCGTGG TGCTTGTAGG CCACTCTATG GGTGGGGTGG TGGCCCGAGC CCTGTTCACA 
CTGCCCCGCT TCAACGCTCG CCTGGTCAGC CTGATCCTCA CACAGGCCTC GCCGCACCAG 
GCTCCGGTTC TGGCTCTGGA CCCGTACCTG CTGGATTTCT ATTCTACCGT GAGGCACAGG 
TGGGTGAACC AAGCAAACAA GCTGACGAAT GTTACCGTTC TCTCTGTTGG GGGCGGTTAC 
CGTGACTACC AAGTCCGCTC CGGCCTCACG TCACTTCCTT GTCCCTCAGA AGACTCAAAT 
AAGTTCTCAT TGGTGGTAAC CGCTGTCCCA AGAACATGGG TGTCCACCGA CCACCTGTCT 
ATTGTTTGGT GCAAAGAGCT TGTTCTCGCC ACCGTTAGGA CTTTTTTTGA CCTCATTGAT 
CTTGAAACCC GACAGTTCAC AGAGAACCAG GAGAGGAAAG TGGCCGTTCT GAACCATCAT 
TTCATCAGAA ATCCTGCCAG ATTTGTGAAG GAAAGTCCGG AAACATCTTC CTCCGTTCAA 
GATATTCAGG AAACAGGAAG TGAAGTGAAC ACCCTGCGCC TGACCTACAG CACACCGAAG 
GAAGGACCAG TGAAGAACTT TGTGTTTGCC CTGTCGAGTC GCAGGAAGGC CTACAGCCAC 
TTCTACTGCC GCAGCAACAA CCTGGAGACG TCAAGCTGGG TGTTTGGCTG CACGCGAAAG 
AACGGCTCTT CTTGTGTCAA CACTGAGGAT CTCTCTAAAG GAACCGAACT TCTACCCCCA 
TACAAGGTCC TCGTTCTGAG TCTCCATGAC CTTTCTGCTT TTTCTCACCT GGTTGTTTCC 
GCCTCCAACA TCAACGGGAA ACAGTTCACA GTGGAGTGTG AGTGGCAGAG ACATCAGTCG 
GACTCTCAGA CCCTAACGCT CTCCGTTCCC AGCGTCTTCT CCTTCGGTTT CACCTCCAGT 
GAAGTTACGG TAAACTCTTC CGGACTTCTG CACCACATTC GTCTGCAGCA CTTTCATCAG 
GCCTACCAGG CTTTCAGGAT TAATGTCATC AGTCAGTGCA AAGCTCTGAA AGACCGATTA 
CCCAGTGTGT ACCGGATGAG GGTCCCCTGG TTCAGAGAGG ACTCTCTGAC CACCGTCAGT 
GTCCCAGCAG TGACGGAGAT CTCAGGGATG CTGCACACCA GTCGTCCTGA CAACAGCTCA 
GGTGTGCTCC TAGAGCTCCA CACTGCCCCC AACTGCCAGT ATAAGGTTTC AGTTAGAAGC 
TCATTGCCTA AAGTGCTGGG ACAGGTCCTG AGATTCTGTG GCCCCACGCT GCCGGTCTAC 
ACTGCTGTAA CGCTCCTCCT GGCCTGCAGG GGGCAGCTGT CGTCCATCCT AAAGAGGAGC 
CGAACCGTTG ACATGAGCCA AGCGGCGGCC GCAGGGCTGC AGCCTCACAA GATCAACCTG 
CCCGTTTATG TTCTGCACGT CTTGCTGTGC TGCAGTTGGT TTCAGGAGGT CTGGTCAAGT 
CTTTGGCTGC CACCCATGGA TACAATCCCT CCATCATCCC CTGATGGGAT GTTTCGTGAG 
GGCATGGCTC CAGCTGAGGA GTGGCCTCAC CTCCTATCTC CTTTCCTTTA TGTCCTGGGG 
GCAGCTGTGG CGTTCTGGGG CAGCTCGCTG CTCCGCCTCC TCATACGCCT GCTCTCACTG 
CTGCTGGCTC CTCTGCACAG GTCGTCTGTG TCTGGAGATG GTGGCACGAT GGGACCACGG 
GTTCAGATCT TCATCAGCCT CTGTCTGACT GTTGTGGGCG GGGTTTCCTG TGGGGCGTTG 
GCGATCGCTG CCTCCTCTCT TCTCCACCTT TACAGGGTTC TGCGGCTGCA GATGACGGAG 
AGGTATTTGA GTCACATGCT GAATCTGGCA CCGCAGAAGC ACAAACTAAC GGAGAATGGC 
ACAGTCCGAG AAGAGTCCTC AAGCAAGTCT AAACCAGGCT TGCTGTCTGA GTGGGCCCTG 
CAGGAGGTAA GGGACAACCT GGAGCTGCAC CTCTGCCTGT CGGCACTCTT CACGCTTCCC 
GTCATGCTAA GTGCGCCCTC ACTCCTCCAC TGGATCGGCA ACCTGAGATA TGCCTCTCAG 
CTGGATCCTG ATCCTTGCTG GCCTCACACT GTGCCTCTGG TTATCGCGAA CCTGCTGCTC 
GTCAACTGCA CCACCCTCAA ACTCAGCAAC AGCAAACTCC TGCCTCTGGC CTCCTGCCTC 
CCGCTGCCCC TGGCCGTCAC CATGGTGACC TTCTCCTCCC TTCACCTGTA CCGAATCACC 
CACTTCCTGT CAGCAGCTCT CATCCCGCTG GCCTTGTGTT GTCTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004082094.1
CDS	135..2945
Translation	MNLPAVAFYALAAGLLAVGLREVLTGFEENRCSMTYMFEYPEYRRVALPRRVARMYPAYGLYLYGEGLYAQETQVLKLTGAPVLFLPGNAGSYKQARSLGSVALRKAENVDGGLHLNMFTVDFNEELVALYGGSLFRQTHFLHESIKAILRLYKHLKTPPQSVVLVGHSMGGVVARALFTLPRFNARLVSLILTQASPHQAPVLALDPYLLDFYSTVRHRWVNQANKLTNVTVLSVGGGYRDYQVRSGLTSLPCPSEDSNKFSLVVTAVPRTWVSTDHLSIVWCKELVLATVRTFFDLIDLETRQFTENQERKVAVLNHHFIRNPARFVKESPETSSSVQDIQETGSEVNTLRLTYSTPKEGPVKNFVFALSSRRKAYSHFYCRSNNLETSSWVFGCTRKNGSSCVNTEDLSKGTELLPPYKVLVLSLHDLSAFSHLVVSASNINGKQFTVECEWQRHQSDSQTLTLSVPSVFSFGFTSSEVTVNSSGLLHHIRLQHFHQAYQAFRINVISQCKALKDRLPSVYRMRVPWFREDSLTTVSVPAVTEISGMLHTSRPDNSSGVLLELHTAPNCQYKVSVRSSLPKVLGQVLRFCGPTLPVYTAVTLLLACRGQLSSILKRSRTVDMSQAAAAGLQPHKINLPVYVLHVLLCCSWFQEVWSSLWLPPMDTIPPSSPDGMFREGMAPAEEWPHLLSPFLYVLGAAVAFWGSSLLRLLIRLLSLLLAPLHRSSVSGDGGTMGPRVQIFISLCLTVVGGVSCGALAIAASSLLHLYRVLRLQMTERYLSHMLNLAPQKHKLTENGTVREESSSKSKPGLLSEWALQEVRDNLELHLCLSALFTLPVMLSAPSLLHWIGNLRYASQLDPDPCWPHTVPLVIANLLLVNCTTLKLSNSKLLPLASCLPLPLAVTMVTFSSLHLYRITHFLSAALIPLALCCLL

Target ORF information:

RefSeq Version	XM_004082046.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes post-GPI attachment to proteins 1 (pgap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004082046.3

ORF Insert Sequence:

ATGAATTTGC CCGCCGTGGC CTTTTACGCG CTGGCCGCGG GGCTCCTGGC GGTGGGCCTG 
CGGGAGGTCC TGACGGGCTT CGAGGAGAAC CGCTGCAGCA TGACCTACAT GTTCGAATAC 
CCGGAGTACC GCCGGGTGGC GCTGCCTCGC CGCGTGGCCA GAATGTACCC GGCGTACGGG 
CTTTACCTGT ACGGAGAGGG CCTGTATGCG CAGGAGACCC AGGTGCTCAA ACTCACCGGC 
GCACCTGTTC TCTTCTTACC TGGAAATGCA GGAAGTTACA AACAAGCTCG CTCGCTGGGA 
TCTGTGGCCT TGAGGAAAGC GGAGAACGTG GACGGGGGGC TCCACCTGAA CATGTTCACT 
GTGGACTTCA ACGAGGAGCT GGTGGCTCTG TACGGTGGGA GTTTGTTCAG ACAAACCCAC 
TTCCTGCACG AGAGCATCAA GGCCATCCTG CGGCTCTATA AGCACCTGAA GACCCCCCCT 
CAGAGCGTGG TGCTTGTAGG CCACTCTATG GGTGGGGTGG TGGCCCGAGC CCTGTTCACA 
CTGCCCCGCT TCAACGCTCG CCTGGTCAGC CTGATCCTCA CACAGGCCTC GCCGCACCAG 
GCTCCGGTTC TGGCTCTGGA CCCGTACCTG CTGGATTTCT ATTCTACCGT GAGGCACAGG 
TGGGTGAACC AAGCAAACAA GCTGACGAAT GTTACCGTTC TCTCTGTTGG GGGCGGTTAC 
CGTGACTACC AAGTCCGCTC CGGCCTCACG TCACTTCCTT GTCCCTCAGA AGACTCAAAT 
AAGTTCTCAT TGGTGGTAAC CGCTGTCCCA AGAACATGGG TGTCCACCGA CCACCTGTCT 
ATTGTTTGGT GCAAAGAGCT TGTTCTCGCC ACCGTTAGGA CTTTTTTTGA CCTCATTGAT 
CTTGAAACCC GACAGTTCAC AGAGAACCAG GAGAGGAAAG TGGCCGTTCT GAACCATCAT 
TTCATCAGAA ATCCTGCCAG ATTTGTGAAG GAAAGTCCGG AAACATCTTC CTCCGTTCAA 
GATATTCAGG AAACAGGAAG TGAAGTGAAC ACCCTGCGCC TGACCTACAG CACACCGAAG 
GAAGGACCAG TGAAGAACTT TGTGTTTGCC CTGTCGAGTC GCAGGAAGGC CTACAGCCAC 
TTCTACTGCC GCAGCAACAA CCTGGAGACG TCAAGCTGGG TGTTTGGCTG CACGCGAAAG 
AACGGCTCTT CTTGTGTCAA CACTGAGGAT CTCTCTAAAG GAACCGAACT TCTACCCCCA 
TACAAGGTCC TCGTTCTGAG TCTCCATGAC CTTTCTGCTT TTTCTCACCT GGTTGTTTCC 
GCCTCCAACA TCAACGGGAA ACAGTTCACA GTGGAGTGTG AGTGGCAGAG ACATCAGTCG 
GACTCTCAGA CCCTAACGCT CTCCGTTCCC AGCGTCTTCT CCTTCGGTTT CACCTCCAGT 
GAAGTTACGG TAAACTCTTC CGGACTTCTG CACCACATTC GTCTGCAGCA CTTTCATCAG 
GCCTACCAGG CTTTCAGGAT TAATGTCATC AGTCAGTGCA AAGCTCTGAA AGACCGATTA 
CCCAGTGTGT ACCGGATGAG GGTCCCCTGG TTCAGAGAGG ACTCTCTGAC CACCGTCAGT 
GTCCCAGCAG TGACGGAGAT CTCAGGGATG CTGCACACCA GTCGTCCTGA CAACAGCTCA 
GGTGTGCTCC TAGAGCTCCA CACTGCCCCC AACTGCCAGT ATAAGGTTTC AGTTAGAAGC 
TCATTGCCTA AAGTGCTGGG ACAGGTCCTG AGATTCTGTG GCCCCACGCT GCCGGTCTAC 
ACTGCTGTAA CGCTCCTCCT GGCCTGCAGG GGGCAGCTGT CGTCCATCCT AAAGAGGAGC 
CGAACCGTTG ACATGAGCCA AGCGGCGGCC GCAGGGCTGC AGCCTCACAA GATCAACCTG 
CCCGTTTATG TTCTGCACGT CTTGCTGTGC TGCAGTTGGT TTCAGGAGGT CTGGTCAAGT 
CTTTGGCTGC CACCCATGGA TACAATCCCT CCATCATCCC CTGATGGGAT GTTTCGTGAG 
GGCATGGCTC CAGCTGAGGA GTGGCCTCAC CTCCTATCTC CTTTCCTTTA TGTCCTGGGG 
GCAGCTGTGG CGTTCTGGGG CAGCTCGCTG CTCCGCCTCC TCATACGCCT GCTCTCACTG 
CTGCTGGCTC CTCTGCACAG GTCGTCTGTG TCTGGAGATG GTGGCACGAT GGGACCACGG 
GTTCAGATCT TCATCAGCCT CTGTCTGACT GTTGTGGGCG GGGTTTCCTG TGGGGCGTTG 
GCGATCGCTG CCTCCTCTCT TCTCCACCTT TACAGGGTTC TGCGGCTGCA GATGACGGAG 
AGGTATTTGA GTCACATGCT GAATCTGGCA CCGCAGAAGC ACAAACTAAC GGAGAATGGC 
ACAGTCCGAG AAGAGTCCTC AAGCAAGTCT AAACCAGGCT TGCTGTCTGA GTGGGCCCTG 
CAGGAGGTAA GGGACAACCT GGAGCTGCAC CTCTGCCTGT CGGCACTCTT CACGCTTCCC 
GTCATGCTAA GTGCGCCCTC ACTCCTCCAC TGGATCGGCA ACCTGAGATA TGCCTCTCAG 
CTGGATCCTG ATCCTTGCTG GCCTCACACT GTGCCTCTGG TTATCGCGAA CCTGCTGCTC 
GTCAACTGCA CCACCCTCAA ACTCAGCAAC AGCAAACTCC TGCCTCTGGC CTCCTGCCTC 
CCGCTGCCCC TGGCCGTCAC CATGGTGACC TTCTCCTCCC TTCACCTGTA CCGAATCACC 
CACTTCCTGT CAGCAGCTCT CATCCCGCTG GCCTTGTGTT GTCTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pgap1 ( XM_004082046.4 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_004082094.1 Oryzias latipes post-GPI attachment to proteins 1 (pgap1), mRNA.

Price & Availability↑

CloneID	OJa16215
Clone ID Related Accession (Same CDS sequence)	XM_004082046.3 , XM_004082046.4
Accession Version	XM_004082046.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	GPI inositol-deacylase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019879.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_004082046.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTGC CCGCCGTGGC CTTTTACGCG CTGGCCGCGG GGCTCCTGGC GGTGGGCCTG 
CGGGAGGTCC TGACGGGCTT CGAGGAGAAC CGCTGCAGCA TGACCTACAT GTTCGAATAC 
CCGGAGTACC GCCGGGTGGC GCTGCCTCGC CGCGTGGCCA GAATGTACCC GGCGTACGGG 
CTTTACCTGT ACGGAGAGGG CCTGTATGCG CAGGAGACCC AGGTGCTCAA ACTCACCGGC 
GCACCTGTTC TCTTCTTACC TGGAAATGCA GGAAGTTACA AACAAGCTCG CTCGCTGGGA 
TCTGTGGCCT TGAGGAAAGC GGAGAACGTG GACGGGGGGC TCCACCTGAA CATGTTCACT 
GTGGACTTCA ACGAGGAGCT GGTGGCTCTG TACGGTGGGA GTTTGTTCAG ACAAACCCAC 
TTCCTGCACG AGAGCATCAA GGCCATCCTG CGGCTCTATA AGCACCTGAA GACCCCCCCT 
CAGAGCGTGG TGCTTGTAGG CCACTCTATG GGTGGGGTGG TGGCCCGAGC CCTGTTCACA 
CTGCCCCGCT TCAACGCTCG CCTGGTCAGC CTGATCCTCA CACAGGCCTC GCCGCACCAG 
GCTCCGGTTC TGGCTCTGGA CCCGTACCTG CTGGATTTCT ATTCTACCGT GAGGCACAGG 
TGGGTGAACC AAGCAAACAA GCTGACGAAT GTTACCGTTC TCTCTGTTGG GGGCGGTTAC 
CGTGACTACC AAGTCCGCTC CGGCCTCACG TCACTTCCTT GTCCCTCAGA AGACTCAAAT 
AAGTTCTCAT TGGTGGTAAC CGCTGTCCCA AGAACATGGG TGTCCACCGA CCACCTGTCT 
ATTGTTTGGT GCAAAGAGCT TGTTCTCGCC ACCGTTAGGA CTTTTTTTGA CCTCATTGAT 
CTTGAAACCC GACAGTTCAC AGAGAACCAG GAGAGGAAAG TGGCCGTTCT GAACCATCAT 
TTCATCAGAA ATCCTGCCAG ATTTGTGAAG GAAAGTCCGG AAACATCTTC CTCCGTTCAA 
GATATTCAGG AAACAGGAAG TGAAGTGAAC ACCCTGCGCC TGACCTACAG CACACCGAAG 
GAAGGACCAG TGAAGAACTT TGTGTTTGCC CTGTCGAGTC GCAGGAAGGC CTACAGCCAC 
TTCTACTGCC GCAGCAACAA CCTGGAGACG TCAAGCTGGG TGTTTGGCTG CACGCGAAAG 
AACGGCTCTT CTTGTGTCAA CACTGAGGAT CTCTCTAAAG GAACCGAACT TCTACCCCCA 
TACAAGGTCC TCGTTCTGAG TCTCCATGAC CTTTCTGCTT TTTCTCACCT GGTTGTTTCC 
GCCTCCAACA TCAACGGGAA ACAGTTCACA GTGGAGTGTG AGTGGCAGAG ACATCAGTCG 
GACTCTCAGA CCCTAACGCT CTCCGTTCCC AGCGTCTTCT CCTTCGGTTT CACCTCCAGT 
GAAGTTACGG TAAACTCTTC CGGACTTCTG CACCACATTC GTCTGCAGCA CTTTCATCAG 
GCCTACCAGG CTTTCAGGAT TAATGTCATC AGTCAGTGCA AAGCTCTGAA AGACCGATTA 
CCCAGTGTGT ACCGGATGAG GGTCCCCTGG TTCAGAGAGG ACTCTCTGAC CACCGTCAGT 
GTCCCAGCAG TGACGGAGAT CTCAGGGATG CTGCACACCA GTCGTCCTGA CAACAGCTCA 
GGTGTGCTCC TAGAGCTCCA CACTGCCCCC AACTGCCAGT ATAAGGTTTC AGTTAGAAGC 
TCATTGCCTA AAGTGCTGGG ACAGGTCCTG AGATTCTGTG GCCCCACGCT GCCGGTCTAC 
ACTGCTGTAA CGCTCCTCCT GGCCTGCAGG GGGCAGCTGT CGTCCATCCT AAAGAGGAGC 
CGAACCGTTG ACATGAGCCA AGCGGCGGCC GCAGGGCTGC AGCCTCACAA GATCAACCTG 
CCCGTTTATG TTCTGCACGT CTTGCTGTGC TGCAGTTGGT TTCAGGAGGT CTGGTCAAGT 
CTTTGGCTGC CACCCATGGA TACAATCCCT CCATCATCCC CTGATGGGAT GTTTCGTGAG 
GGCATGGCTC CAGCTGAGGA GTGGCCTCAC CTCCTATCTC CTTTCCTTTA TGTCCTGGGG 
GCAGCTGTGG CGTTCTGGGG CAGCTCGCTG CTCCGCCTCC TCATACGCCT GCTCTCACTG 
CTGCTGGCTC CTCTGCACAG GTCGTCTGTG TCTGGAGATG GTGGCACGAT GGGACCACGG 
GTTCAGATCT TCATCAGCCT CTGTCTGACT GTTGTGGGCG GGGTTTCCTG TGGGGCGTTG 
GCGATCGCTG CCTCCTCTCT TCTCCACCTT TACAGGGTTC TGCGGCTGCA GATGACGGAG 
AGGTATTTGA GTCACATGCT GAATCTGGCA CCGCAGAAGC ACAAACTAAC GGAGAATGGC 
ACAGTCCGAG AAGAGTCCTC AAGCAAGTCT AAACCAGGCT TGCTGTCTGA GTGGGCCCTG 
CAGGAGGTAA GGGACAACCT GGAGCTGCAC CTCTGCCTGT CGGCACTCTT CACGCTTCCC 
GTCATGCTAA GTGCGCCCTC ACTCCTCCAC TGGATCGGCA ACCTGAGATA TGCCTCTCAG 
CTGGATCCTG ATCCTTGCTG GCCTCACACT GTGCCTCTGG TTATCGCGAA CCTGCTGCTC 
GTCAACTGCA CCACCCTCAA ACTCAGCAAC AGCAAACTCC TGCCTCTGGC CTCCTGCCTC 
CCGCTGCCCC TGGCCGTCAC CATGGTGACC TTCTCCTCCC TTCACCTGTA CCGAATCACC 
CACTTCCTGT CAGCAGCTCT CATCCCGCTG GCCTTGTGTT GTCTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004082094.1
CDS	128..2938
Translation	MNLPAVAFYALAAGLLAVGLREVLTGFEENRCSMTYMFEYPEYRRVALPRRVARMYPAYGLYLYGEGLYAQETQVLKLTGAPVLFLPGNAGSYKQARSLGSVALRKAENVDGGLHLNMFTVDFNEELVALYGGSLFRQTHFLHESIKAILRLYKHLKTPPQSVVLVGHSMGGVVARALFTLPRFNARLVSLILTQASPHQAPVLALDPYLLDFYSTVRHRWVNQANKLTNVTVLSVGGGYRDYQVRSGLTSLPCPSEDSNKFSLVVTAVPRTWVSTDHLSIVWCKELVLATVRTFFDLIDLETRQFTENQERKVAVLNHHFIRNPARFVKESPETSSSVQDIQETGSEVNTLRLTYSTPKEGPVKNFVFALSSRRKAYSHFYCRSNNLETSSWVFGCTRKNGSSCVNTEDLSKGTELLPPYKVLVLSLHDLSAFSHLVVSASNINGKQFTVECEWQRHQSDSQTLTLSVPSVFSFGFTSSEVTVNSSGLLHHIRLQHFHQAYQAFRINVISQCKALKDRLPSVYRMRVPWFREDSLTTVSVPAVTEISGMLHTSRPDNSSGVLLELHTAPNCQYKVSVRSSLPKVLGQVLRFCGPTLPVYTAVTLLLACRGQLSSILKRSRTVDMSQAAAAGLQPHKINLPVYVLHVLLCCSWFQEVWSSLWLPPMDTIPPSSPDGMFREGMAPAEEWPHLLSPFLYVLGAAVAFWGSSLLRLLIRLLSLLLAPLHRSSVSGDGGTMGPRVQIFISLCLTVVGGVSCGALAIAASSLLHLYRVLRLQMTERYLSHMLNLAPQKHKLTENGTVREESSSKSKPGLLSEWALQEVRDNLELHLCLSALFTLPVMLSAPSLLHWIGNLRYASQLDPDPCWPHTVPLVIANLLLVNCTTLKLSNSKLLPLASCLPLPLAVTMVTFSSLHLYRITHFLSAALIPLALCCLL

Target ORF information:

RefSeq Version	XM_004082046.4
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes post-GPI attachment to proteins 1 (pgap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004082046.4

ORF Insert Sequence:

ATGAATTTGC CCGCCGTGGC CTTTTACGCG CTGGCCGCGG GGCTCCTGGC GGTGGGCCTG 
CGGGAGGTCC TGACGGGCTT CGAGGAGAAC CGCTGCAGCA TGACCTACAT GTTCGAATAC 
CCGGAGTACC GCCGGGTGGC GCTGCCTCGC CGCGTGGCCA GAATGTACCC GGCGTACGGG 
CTTTACCTGT ACGGAGAGGG CCTGTATGCG CAGGAGACCC AGGTGCTCAA ACTCACCGGC 
GCACCTGTTC TCTTCTTACC TGGAAATGCA GGAAGTTACA AACAAGCTCG CTCGCTGGGA 
TCTGTGGCCT TGAGGAAAGC GGAGAACGTG GACGGGGGGC TCCACCTGAA CATGTTCACT 
GTGGACTTCA ACGAGGAGCT GGTGGCTCTG TACGGTGGGA GTTTGTTCAG ACAAACCCAC 
TTCCTGCACG AGAGCATCAA GGCCATCCTG CGGCTCTATA AGCACCTGAA GACCCCCCCT 
CAGAGCGTGG TGCTTGTAGG CCACTCTATG GGTGGGGTGG TGGCCCGAGC CCTGTTCACA 
CTGCCCCGCT TCAACGCTCG CCTGGTCAGC CTGATCCTCA CACAGGCCTC GCCGCACCAG 
GCTCCGGTTC TGGCTCTGGA CCCGTACCTG CTGGATTTCT ATTCTACCGT GAGGCACAGG 
TGGGTGAACC AAGCAAACAA GCTGACGAAT GTTACCGTTC TCTCTGTTGG GGGCGGTTAC 
CGTGACTACC AAGTCCGCTC CGGCCTCACG TCACTTCCTT GTCCCTCAGA AGACTCAAAT 
AAGTTCTCAT TGGTGGTAAC CGCTGTCCCA AGAACATGGG TGTCCACCGA CCACCTGTCT 
ATTGTTTGGT GCAAAGAGCT TGTTCTCGCC ACCGTTAGGA CTTTTTTTGA CCTCATTGAT 
CTTGAAACCC GACAGTTCAC AGAGAACCAG GAGAGGAAAG TGGCCGTTCT GAACCATCAT 
TTCATCAGAA ATCCTGCCAG ATTTGTGAAG GAAAGTCCGG AAACATCTTC CTCCGTTCAA 
GATATTCAGG AAACAGGAAG TGAAGTGAAC ACCCTGCGCC TGACCTACAG CACACCGAAG 
GAAGGACCAG TGAAGAACTT TGTGTTTGCC CTGTCGAGTC GCAGGAAGGC CTACAGCCAC 
TTCTACTGCC GCAGCAACAA CCTGGAGACG TCAAGCTGGG TGTTTGGCTG CACGCGAAAG 
AACGGCTCTT CTTGTGTCAA CACTGAGGAT CTCTCTAAAG GAACCGAACT TCTACCCCCA 
TACAAGGTCC TCGTTCTGAG TCTCCATGAC CTTTCTGCTT TTTCTCACCT GGTTGTTTCC 
GCCTCCAACA TCAACGGGAA ACAGTTCACA GTGGAGTGTG AGTGGCAGAG ACATCAGTCG 
GACTCTCAGA CCCTAACGCT CTCCGTTCCC AGCGTCTTCT CCTTCGGTTT CACCTCCAGT 
GAAGTTACGG TAAACTCTTC CGGACTTCTG CACCACATTC GTCTGCAGCA CTTTCATCAG 
GCCTACCAGG CTTTCAGGAT TAATGTCATC AGTCAGTGCA AAGCTCTGAA AGACCGATTA 
CCCAGTGTGT ACCGGATGAG GGTCCCCTGG TTCAGAGAGG ACTCTCTGAC CACCGTCAGT 
GTCCCAGCAG TGACGGAGAT CTCAGGGATG CTGCACACCA GTCGTCCTGA CAACAGCTCA 
GGTGTGCTCC TAGAGCTCCA CACTGCCCCC AACTGCCAGT ATAAGGTTTC AGTTAGAAGC 
TCATTGCCTA AAGTGCTGGG ACAGGTCCTG AGATTCTGTG GCCCCACGCT GCCGGTCTAC 
ACTGCTGTAA CGCTCCTCCT GGCCTGCAGG GGGCAGCTGT CGTCCATCCT AAAGAGGAGC 
CGAACCGTTG ACATGAGCCA AGCGGCGGCC GCAGGGCTGC AGCCTCACAA GATCAACCTG 
CCCGTTTATG TTCTGCACGT CTTGCTGTGC TGCAGTTGGT TTCAGGAGGT CTGGTCAAGT 
CTTTGGCTGC CACCCATGGA TACAATCCCT CCATCATCCC CTGATGGGAT GTTTCGTGAG 
GGCATGGCTC CAGCTGAGGA GTGGCCTCAC CTCCTATCTC CTTTCCTTTA TGTCCTGGGG 
GCAGCTGTGG CGTTCTGGGG CAGCTCGCTG CTCCGCCTCC TCATACGCCT GCTCTCACTG 
CTGCTGGCTC CTCTGCACAG GTCGTCTGTG TCTGGAGATG GTGGCACGAT GGGACCACGG 
GTTCAGATCT TCATCAGCCT CTGTCTGACT GTTGTGGGCG GGGTTTCCTG TGGGGCGTTG 
GCGATCGCTG CCTCCTCTCT TCTCCACCTT TACAGGGTTC TGCGGCTGCA GATGACGGAG 
AGGTATTTGA GTCACATGCT GAATCTGGCA CCGCAGAAGC ACAAACTAAC GGAGAATGGC 
ACAGTCCGAG AAGAGTCCTC AAGCAAGTCT AAACCAGGCT TGCTGTCTGA GTGGGCCCTG 
CAGGAGGTAA GGGACAACCT GGAGCTGCAC CTCTGCCTGT CGGCACTCTT CACGCTTCCC 
GTCATGCTAA GTGCGCCCTC ACTCCTCCAC TGGATCGGCA ACCTGAGATA TGCCTCTCAG 
CTGGATCCTG ATCCTTGCTG GCCTCACACT GTGCCTCTGG TTATCGCGAA CCTGCTGCTC 
GTCAACTGCA CCACCCTCAA ACTCAGCAAC AGCAAACTCC TGCCTCTGGC CTCCTGCCTC 
CCGCTGCCCC TGGCCGTCAC CATGGTGACC TTCTCCTCCC TTCACCTGTA CCGAATCACC 
CACTTCCTGT CAGCAGCTCT CATCCCGCTG GCCTTGTGTT GTCTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pgap1 ( XM_020713057.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020568716.1 Oryzias latipes post-GPI attachment to proteins 1 (pgap1), transcript variant X2, mRNA.

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CloneID	OJa75336
Clone ID Related Accession (Same CDS sequence)	XM_020713057.1
Accession Version	XM_020713057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	GPI inositol-deacylase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019879.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCGG CGTACGGGCT TTACCTGTAC GGAGAGGGCC TGTATGCGCA GGAGACCCAG 
GTGCTCAAAC TCACCGGCGC ACCTGTTCTC TTCTTACCTG GAAATGCAGG AAGTTACAAA 
CAAGCTCGCT CGCTGGGATC TGTGGCCTTG AGGAAAGCGG AGAACGTGGA CGGGGGGCTC 
CACCTGAACA TGTTCACTGT GGACTTCAAC GAGGAGCTGG TGGCTCTGTA CGGTGGGAGT 
TTGTTCAGAC AAACCCACTT CCTGCACGAG AGCATCAAGG CCATCCTGCG GCTCTATAAG 
CACCTGAAGA CCCCCCCTCA GAGCGTGGTG CTTGTAGGCC ACTCTATGGG TGGGGTGGTG 
GCCCGAGCCC TGTTCACACT GCCCCGCTTC AACGCTCGCC TGGTCAGCCT GATCCTCACA 
CAGGCCTCGC CGCACCAGGC TCCGGTTCTG GCTCTGGACC CGTACCTGCT GGATTTCTAT 
TCTACCGTGA GGCACAGGTG GGTGAACCAA GCAAACAAGC TGACGAATGT TACCGTTCTC 
TCTGTTGGGG GCGGTTACCG TGACTACCAA GTCCGCTCCG GCCTCACGTC ACTTCCTTGT 
CCCTCAGAAG ACTCAAATAA GTTCTCATTG GTGGTAACCG CTGTCCCAAG AACATGGGTG 
TCCACCGACC ACCTGTCTAT TGTTTGGTGC AAAGAGCTTG TTCTCGCCAC CGTTAGGACT 
TTTTTTGACC TCATTGATCT TGAAACCCGA CAGTTCACAG AGAACCAGGA GAGGAAAGTG 
GCCGTTCTGA ACCATCATTT CATCAGAAAT CCTGCCAGAT TTGTGAAGGA AAGTCCGGAA 
ACATCTTCCT CCGTTCAAGA TATTCAGGAA ACAGGAAGTG AAGTGAACAC CCTGCGCCTG 
ACCTACAGCA CACCGAAGGA AGGACCAGTG AAGAACTTTG TGTTTGCCCT GTCGAGTCGC 
AGGAAGGCCT ACAGCCACTT CTACTGCCGC AGCAACAACC TGGAGACGTC AAGCTGGGTG 
TTTGGCTGCA CGCGAAAGAA CGGCTCTTCT TGTGTCAACA CTGAGGATCT CTCTAAAGGA 
ACCGAACTTC TACCCCCATA CAAGGTCCTC GTTCTGAGTC TCCATGACCT TTCTGCTTTT 
TCTCACCTGG TTGTTTCCGC CTCCAACATC AACGGGAAAC AGTTCACAGT GGAGTGTGAG 
TGGCAGAGAC ATCAGTCGGA CTCTCAGACC CTAACGCTCT CCGTTCCCAG CGTCTTCTCC 
TTCGGTTTCA CCTCCAGTGA AGTTACGGTA AACTCTTCCG GACTTCTGCA CCACATTCGT 
CTGCAGCACT TTCATCAGGC CTACCAGGCT TTCAGGATTA ATGTCATCAG TCAGTGCAAA 
GCTCTGAAAG ACCGATTACC CAGTGTGTAC CGGATGAGGG TCCCCTGGTT CAGAGAGGAC 
TCTCTGACCA CCGTCAGTGT CCCAGCAGTG ACGGAGATCT CAGGGATGCT GCACACCAGT 
CGTCCTGACA ACAGCTCAGG TGTGCTCCTA GAGCTCCACA CTGCCCCCAA CTGCCAGTAT 
AAGGTTTCAG TTAGAAGCTC ATTGCCTAAA GTGCTGGGAC AGGTCCTGAG ATTCTGTGGC 
CCCACGCTGC CGGTCTACAC TGCTGTAACG CTCCTCCTGG CCTGCAGGGG GCAGCTGTCG 
TCCATCCTAA AGAGGAGCCG AACCGTTGAC ATGAGCCAAG CGGCGGCCGC AGGGCTGCAG 
CCTCACAAGA TCAACCTGCC CGTTTATGTT CTGCACGTCT TGCTGTGCTG CAGTTGGTTT 
CAGGAGGTCT GGTCAAGTCT TTGGCTGCCA CCCATGGATA CAATCCCTCC ATCATCCCCT 
GATGGGATGT TTCGTGAGGG CATGGCTCCA GCTGAGGAGT GGCCTCACCT CCTATCTCCT 
TTCCTTTATG TCCTGGGGGC AGCTGTGGCG TTCTGGGGCA GCTCGCTGCT CCGCCTCCTC 
ATACGCCTGC TCTCACTGCT GCTGGCTCCT CTGCACAGGT CGTCTGTGTC TGGAGATGGT 
GGCACGATGG GACCACGGGT TCAGATCTTC ATCAGCCTCT GTCTGACTGT TGTGGGCGGG 
GTTTCCTGTG GGGCGTTGGC GATCGCTGCC TCCTCTCTTC TCCACCTTTA CAGGGTTCTG 
CGGCTGCAGA TGACGGAGAG GTATTTGAGT CACATGCTGA ATCTGGCACC GCAGAAGCAC 
AAACTAACGG AGAATGGCAC AGTCCGAGAA GAGTCCTCAA GCAAGTCTAA ACCAGGCTTG 
CTGTCTGAGT GGGCCCTGCA GGAGGTAAGG GACAACCTGG AGCTGCACCT CTGCCTGTCG 
GCACTCTTCA CGCTTCCCGT CATGCTAAGT GCGCCCTCAC TCCTCCACTG GATCGGCAAC 
CTGAGATATG CCTCTCAGCT GGATCCTGAT CCTTGCTGGC CTCACACTGT GCCTCTGGTT 
ATCGCGAACC TGCTGCTCGT CAACTGCACC ACCCTCAAAC TCAGCAACAG CAAACTCCTG 
CCTCTGGCCT CCTGCCTCCC GCTGCCCCTG GCCGTCACCA TGGTGACCTT CTCCTCCCTT 
CACCTGTACC GAATCACCCA CTTCCTGTCA GCAGCTCTCA TCCCGCTGGC CTTGTGTTGT 
CTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020568716.1
CDS	416..3064
Translation	MYPAYGLYLYGEGLYAQETQVLKLTGAPVLFLPGNAGSYKQARSLGSVALRKAENVDGGLHLNMFTVDFNEELVALYGGSLFRQTHFLHESIKAILRLYKHLKTPPQSVVLVGHSMGGVVARALFTLPRFNARLVSLILTQASPHQAPVLALDPYLLDFYSTVRHRWVNQANKLTNVTVLSVGGGYRDYQVRSGLTSLPCPSEDSNKFSLVVTAVPRTWVSTDHLSIVWCKELVLATVRTFFDLIDLETRQFTENQERKVAVLNHHFIRNPARFVKESPETSSSVQDIQETGSEVNTLRLTYSTPKEGPVKNFVFALSSRRKAYSHFYCRSNNLETSSWVFGCTRKNGSSCVNTEDLSKGTELLPPYKVLVLSLHDLSAFSHLVVSASNINGKQFTVECEWQRHQSDSQTLTLSVPSVFSFGFTSSEVTVNSSGLLHHIRLQHFHQAYQAFRINVISQCKALKDRLPSVYRMRVPWFREDSLTTVSVPAVTEISGMLHTSRPDNSSGVLLELHTAPNCQYKVSVRSSLPKVLGQVLRFCGPTLPVYTAVTLLLACRGQLSSILKRSRTVDMSQAAAAGLQPHKINLPVYVLHVLLCCSWFQEVWSSLWLPPMDTIPPSSPDGMFREGMAPAEEWPHLLSPFLYVLGAAVAFWGSSLLRLLIRLLSLLLAPLHRSSVSGDGGTMGPRVQIFISLCLTVVGGVSCGALAIAASSLLHLYRVLRLQMTERYLSHMLNLAPQKHKLTENGTVREESSSKSKPGLLSEWALQEVRDNLELHLCLSALFTLPVMLSAPSLLHWIGNLRYASQLDPDPCWPHTVPLVIANLLLVNCTTLKLSNSKLLPLASCLPLPLAVTMVTFSSLHLYRITHFLSAALIPLALCCLL

Target ORF information:

RefSeq Version	XM_020713057.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes post-GPI attachment to proteins 1 (pgap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020713057.1

ORF Insert Sequence:

ATGTACCCGG CGTACGGGCT TTACCTGTAC GGAGAGGGCC TGTATGCGCA GGAGACCCAG 
GTGCTCAAAC TCACCGGCGC ACCTGTTCTC TTCTTACCTG GAAATGCAGG AAGTTACAAA 
CAAGCTCGCT CGCTGGGATC TGTGGCCTTG AGGAAAGCGG AGAACGTGGA CGGGGGGCTC 
CACCTGAACA TGTTCACTGT GGACTTCAAC GAGGAGCTGG TGGCTCTGTA CGGTGGGAGT 
TTGTTCAGAC AAACCCACTT CCTGCACGAG AGCATCAAGG CCATCCTGCG GCTCTATAAG 
CACCTGAAGA CCCCCCCTCA GAGCGTGGTG CTTGTAGGCC ACTCTATGGG TGGGGTGGTG 
GCCCGAGCCC TGTTCACACT GCCCCGCTTC AACGCTCGCC TGGTCAGCCT GATCCTCACA 
CAGGCCTCGC CGCACCAGGC TCCGGTTCTG GCTCTGGACC CGTACCTGCT GGATTTCTAT 
TCTACCGTGA GGCACAGGTG GGTGAACCAA GCAAACAAGC TGACGAATGT TACCGTTCTC 
TCTGTTGGGG GCGGTTACCG TGACTACCAA GTCCGCTCCG GCCTCACGTC ACTTCCTTGT 
CCCTCAGAAG ACTCAAATAA GTTCTCATTG GTGGTAACCG CTGTCCCAAG AACATGGGTG 
TCCACCGACC ACCTGTCTAT TGTTTGGTGC AAAGAGCTTG TTCTCGCCAC CGTTAGGACT 
TTTTTTGACC TCATTGATCT TGAAACCCGA CAGTTCACAG AGAACCAGGA GAGGAAAGTG 
GCCGTTCTGA ACCATCATTT CATCAGAAAT CCTGCCAGAT TTGTGAAGGA AAGTCCGGAA 
ACATCTTCCT CCGTTCAAGA TATTCAGGAA ACAGGAAGTG AAGTGAACAC CCTGCGCCTG 
ACCTACAGCA CACCGAAGGA AGGACCAGTG AAGAACTTTG TGTTTGCCCT GTCGAGTCGC 
AGGAAGGCCT ACAGCCACTT CTACTGCCGC AGCAACAACC TGGAGACGTC AAGCTGGGTG 
TTTGGCTGCA CGCGAAAGAA CGGCTCTTCT TGTGTCAACA CTGAGGATCT CTCTAAAGGA 
ACCGAACTTC TACCCCCATA CAAGGTCCTC GTTCTGAGTC TCCATGACCT TTCTGCTTTT 
TCTCACCTGG TTGTTTCCGC CTCCAACATC AACGGGAAAC AGTTCACAGT GGAGTGTGAG 
TGGCAGAGAC ATCAGTCGGA CTCTCAGACC CTAACGCTCT CCGTTCCCAG CGTCTTCTCC 
TTCGGTTTCA CCTCCAGTGA AGTTACGGTA AACTCTTCCG GACTTCTGCA CCACATTCGT 
CTGCAGCACT TTCATCAGGC CTACCAGGCT TTCAGGATTA ATGTCATCAG TCAGTGCAAA 
GCTCTGAAAG ACCGATTACC CAGTGTGTAC CGGATGAGGG TCCCCTGGTT CAGAGAGGAC 
TCTCTGACCA CCGTCAGTGT CCCAGCAGTG ACGGAGATCT CAGGGATGCT GCACACCAGT 
CGTCCTGACA ACAGCTCAGG TGTGCTCCTA GAGCTCCACA CTGCCCCCAA CTGCCAGTAT 
AAGGTTTCAG TTAGAAGCTC ATTGCCTAAA GTGCTGGGAC AGGTCCTGAG ATTCTGTGGC 
CCCACGCTGC CGGTCTACAC TGCTGTAACG CTCCTCCTGG CCTGCAGGGG GCAGCTGTCG 
TCCATCCTAA AGAGGAGCCG AACCGTTGAC ATGAGCCAAG CGGCGGCCGC AGGGCTGCAG 
CCTCACAAGA TCAACCTGCC CGTTTATGTT CTGCACGTCT TGCTGTGCTG CAGTTGGTTT 
CAGGAGGTCT GGTCAAGTCT TTGGCTGCCA CCCATGGATA CAATCCCTCC ATCATCCCCT 
GATGGGATGT TTCGTGAGGG CATGGCTCCA GCTGAGGAGT GGCCTCACCT CCTATCTCCT 
TTCCTTTATG TCCTGGGGGC AGCTGTGGCG TTCTGGGGCA GCTCGCTGCT CCGCCTCCTC 
ATACGCCTGC TCTCACTGCT GCTGGCTCCT CTGCACAGGT CGTCTGTGTC TGGAGATGGT 
GGCACGATGG GACCACGGGT TCAGATCTTC ATCAGCCTCT GTCTGACTGT TGTGGGCGGG 
GTTTCCTGTG GGGCGTTGGC GATCGCTGCC TCCTCTCTTC TCCACCTTTA CAGGGTTCTG 
CGGCTGCAGA TGACGGAGAG GTATTTGAGT CACATGCTGA ATCTGGCACC GCAGAAGCAC 
AAACTAACGG AGAATGGCAC AGTCCGAGAA GAGTCCTCAA GCAAGTCTAA ACCAGGCTTG 
CTGTCTGAGT GGGCCCTGCA GGAGGTAAGG GACAACCTGG AGCTGCACCT CTGCCTGTCG 
GCACTCTTCA CGCTTCCCGT CATGCTAAGT GCGCCCTCAC TCCTCCACTG GATCGGCAAC 
CTGAGATATG CCTCTCAGCT GGATCCTGAT CCTTGCTGGC CTCACACTGT GCCTCTGGTT 
ATCGCGAACC TGCTGCTCGT CAACTGCACC ACCCTCAAAC TCAGCAACAG CAAACTCCTG 
CCTCTGGCCT CCTGCCTCCC GCTGCCCCTG GCCGTCACCA TGGTGACCTT CTCCTCCCTT 
CACCTGTACC GAATCACCCA CTTCCTGTCA GCAGCTCTCA TCCCGCTGGC CTTGTGTTGT 
CTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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pgap1 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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