clmn cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	clmn
Entrez Gene ID	101171454
Full Name	calmin
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023951390.1	XP_023807158.1	calmin isoform X2
XM_011490151.2	XP_011488453.2	calmin
XM_023951389.1	XP_023807157.1	calmin isoform X1

Pathways from BioSystems
Gene Ontology

The following clmn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the clmn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa113601	XM_023951389.1 Latest version!	Oryzias latipes calmin (clmn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OJa113602	XM_023951390.1 Latest version!	Oryzias latipes calmin (clmn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OJa75646	XM_011490151.2 Latest version!	Oryzias latipes calmin (clmn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

clmn ( XM_023951389.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023807157.1 Oryzias latipes calmin (clmn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OJa113601
Clone ID Related Accession (Same CDS sequence)	XM_023951389.1
Accession Version	XM_023951389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	calmin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGAC ACGAGCGGCA GGACTGGTTC GAGCGCGAGG AATTCATCGG ACAGATCAGC 
GACATACGCG TCCAAAACCT GCAAGTGGAA AGGGAGGCGG TGCAGAAGAG GACCTTCACT 
CGATGGATGA ACCTCCACTT ACAAAAGTGC GACCCGCCCA TCCAGATCCA AGACCTGTTC 
CAGGACATCC AGGATGGGTT TATCCTCATG GTTCTGCTGG AGGAACTCTC CGGCTGCAAA 
CTGATTCAAG GCTTCAAGAA GTCCTCCCAC CGAATTTTCC GACTCAACAA CATTGCCAAA 
GTCTTGGCCT TCCTGGAGGA GCGGAACGTG AAGCTGGTCA GCATTGATGC AGCTGACGTT 
GCTGATGGGA ACTCGTCCAT CATCCTTGGA CTCCTCTGGA ACATCATCCT TTTCTTTCAG 
ATTAAAGAGC TAACTGGAAA CATTCAGAGT CAGTTTCCCT CCTGTTCCAG CCTTTCGTCC 
ATCCCTACGA GCTCTGACTC GGACACGTCC CACAGCAGCA CGCCGTCCGA GGAAAGACGG 
AGCGCCAGCA ACGCTCTCAA GGAGAACAGC AAAGCCATCA AAAAGCTGCT GCAGTGGATT 
CAGAGGAAAA CCCGCAAGTA TGGCGTGGCG GTTCAAGATT TTGGAAGAAG CTGGACAAGT 
GGATTGGCCT TCCTGGCCCT CATTAAGTCC ATTGATTCCA GCCTGGTAGA CATGAGGAAA 
GCCCTACTGA GGAGTTCAAG AGAGAATCTG GAGGATGCCT TCAGGATAGC TCACTACAGC 
CTGGGCATTC CACGCCTGCT GGAGCCCGAG GATGTGGGTT TTAATGAGCC AGACGAGCAG 
TCCATCATCA TGTATGTTTC CCAGTTCTTG GAGCATTTCC CTGGACTTGA GGAGGCGGAG 
GAGTCCACTC AGCTGATTGA GCGGAGCGTC TCCATGGGCC GACTCAACGA CGCAGATCTG 
ATGAGAAACG GCGCCAATCT CAGCAGAGTG AAAGAGCGGT CCAGATTGTT CCAGAGAGAC 
GGCTATAAGC CGCCTCCCAA AGTCCTACTT TCTTCCGTGT CGGAGGACAG AGGCATCCTT 
TCGCCTCGCC TCAGGGTGGC TGCAACTCGC TCCTGGTCCA GTGAAGACGT TCTGTCTGAC 
TCGCCTCTTG ACGTTGTCTC TAATGACGCC CAAGGAGTCG CTGAGGTCCA GCAGGATTTG 
ACCTGCACCT TACCTCAGCA GTCTTTCTCT CATTCACCGT CATTCGCACC GGATTGTGGA 
AACCCAGAGC CTGTAATCCC CGACTCTGCC ATCCACTCCC CAGAATCCTG GATGGAGAGT 
GAGCTGATGC TTGAGACTCA CAGTGACAGT TCTCTGTTTG ACAGCGGGCC GGGCTGGGAT 
GTGTACCGGG CCACACCTGT GCAGGTGACA CCAGTGGATG AAGGATTCAT GTTGTCCATG 
GACGACAGAG CTCCAGATGA ACCCTTTGTG ACGGGATCAT ACAGCGATGA AGGAGTCTCC 
TCGTTGGAGA GCACCCAGGA CAAAGCCCAC CAGGAGGAAC AGGACAAGGA GCTGAACTCA 
AAGAACCAGA ATCTGGTTCA GGAACTCATC ATTGATCCAA GTGAAGTTGA CTTTTCAAAA 
GAGACAGACC TCAGTCCCAT CAGTACAAGT CCACTCCACA AAGACAGTGA GCAGGAACGG 
TCCTGGACAT TCAATGAAGA ATTAACTCTC TCAGGTTCTG AAGATTTCCT TAAAGATGGT 
CAAACAAACC ATTTTCAACC AGCCTTCTCC ACAGATGTTC CACCTCATGA AGAACACACT 
GAAAGTTCTC AGAGAGAACA GGAAGGGAAC AAAAAGAACC TAAATGAACA AACTGATGGT 
CCATCTGGGG AACTACAGGA GAGGGGAACC TATGTGGAAG AGTATCAAAA AGCTGACTCT 
TCAGTGGAAG ATGAAGGAAT CGAGGTAAAA GGTGGAAATG ATCAAGACCA GAAAACCCAG 
GAGGAAGAAC TCTTAGCTAA CGCCTCACAT GATGAAGAAA TCAGTAGTTC TCAGATGATA 
TTAACTAATC AAGAGGGAGC ATCAAAAACT GGTCTAATTA TTCCCATCAT TTCCATCTCC 
AGTGAGCCAA AGGAGCACAA AGAAGAATCT GGCTCAGAAG ACCAGGAAAC AGAGGAGAAA 
CTCCATCAGC AGTTTGAGTC TGGATATGGG GAAGATGACA CGGAATTGAA CAGTCCTACA 
AGTCCAGTTG CTCTGGAGCA TGGTCGTCTG GAGGATGGTC TTTTGGAGTG TGTTCCTCTG 
GAGCACAGTC CTCTAGAGCA TGTCTACAGT GAGGACCTCA AAGAAGAATC TGAGGAGAGA 
CCTTCCTGTC AGATATCAGA AAATTCTGAA AGGAGGCCTT TGCAAACCAC CCACTCTGAA 
GACAAACTAC TGTCTGATGG AGATGAACCT CCCAGAAACT CTGGGTCAGA ATGTGTGCAG 
AACCAGGAAA TGGATGAAGC CAGACAAAAG CACAGCTCAG ATGATTTTGC AGCTGTAAAG 
AAGACAATGG CTCAGGCAAA TCCTGACAGT GATGCAAGTC AGGAACCTGA GGGACATGGA 
GTAAAACCAG CCGGTCCTGC CTCTGAAAGC GGCCCCCAAA TCCCCCATCC GAGCATACTC 
GACACAGACA CTTTAGACCT AAACGAAGCA AAAGTTTCTC CATTTTCTGG ACCACCAGGA 
TTAAACAGAA ACATGGACTT GTTCTATTCC AAATTTGACA AAAGCTCTCC CTCAGAGCAT 
CTAGTTGGTG ACCCAGTGGA ACCCATGGAT CTGTTCTATC CTGACAAAGA GGAGCCCATG 
CTCTCAGAAC CTGCTGACAC GGAGATGCAG AGGTGGCCTT CAGTTCTGAG CGTCTCAGCT 
CTGGAGCCAG CGCCGCCGTC ACTCGATGAC ATGGATGACG AACAGCTGAC GGGAGAGGAG 
GATGATGATC AACAGCTGAA TCCATCCAGC CAGGGGCCTG CTGATGCCTT CTCCACCCAG 
GAGCAGTGCA CGATGTGCGG GGTGCCGATT GGGGAATCCC GGGTGGATTT TCCAGCAGAC 
AGCAGGGAGT CTGAGAGTTG GAGAGATGCG GGAAGTTCCA GCAGAATAGA CGAGATCCAG 
ACCCCTGTAG GTCTCCGGCG CAGGAAGGGT TCTCACCTCG TGGAGTCCAG GGACAACCAG 
AGAGCTGCAT CAAGAACAGC CAACAGAGAT GGCACTTGCT TTTGGTGGAA CGAGAATTCG 
GACCTCTATC TGCTGCTGCT GCTCTGGGTG CTTCTGTACT GCCTCATGCT GCTGCCGCAG 
ATGGACCTAA AGGAGCTGCC GAGTCTTCTG CTGAACCTCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023807157.1
CDS	353..3697
Translation	MARHERQDWFEREEFIGQISDIRVQNLQVEREAVQKRTFTRWMNLHLQKCDPPIQIQDLFQDIQDGFILMVLLEELSGCKLIQGFKKSSHRIFRLNNIAKVLAFLEERNVKLVSIDAADVADGNSSIILGLLWNIILFFQIKELTGNIQSQFPSCSSLSSIPTSSDSDTSHSSTPSEERRSASNALKENSKAIKKLLQWIQRKTRKYGVAVQDFGRSWTSGLAFLALIKSIDSSLVDMRKALLRSSRENLEDAFRIAHYSLGIPRLLEPEDVGFNEPDEQSIIMYVSQFLEHFPGLEEAEESTQLIERSVSMGRLNDADLMRNGANLSRVKERSRLFQRDGYKPPPKVLLSSVSEDRGILSPRLRVAATRSWSSEDVLSDSPLDVVSNDAQGVAEVQQDLTCTLPQQSFSHSPSFAPDCGNPEPVIPDSAIHSPESWMESELMLETHSDSSLFDSGPGWDVYRATPVQVTPVDEGFMLSMDDRAPDEPFVTGSYSDEGVSSLESTQDKAHQEEQDKELNSKNQNLVQELIIDPSEVDFSKETDLSPISTSPLHKDSEQERSWTFNEELTLSGSEDFLKDGQTNHFQPAFSTDVPPHEEHTESSQREQEGNKKNLNEQTDGPSGELQERGTYVEEYQKADSSVEDEGIEVKGGNDQDQKTQEEELLANASHDEEISSSQMILTNQEGASKTGLIIPIISISSEPKEHKEESGSEDQETEEKLHQQFESGYGEDDTELNSPTSPVALEHGRLEDGLLECVPLEHSPLEHVYSEDLKEESEERPSCQISENSERRPLQTTHSEDKLLSDGDEPPRNSGSECVQNQEMDEARQKHSSDDFAAVKKTMAQANPDSDASQEPEGHGVKPAGPASESGPQIPHPSILDTDTLDLNEAKVSPFSGPPGLNRNMDLFYSKFDKSSPSEHLVGDPVEPMDLFYPDKEEPMLSEPADTEMQRWPSVLSVSALEPAPPSLDDMDDEQLTGEEDDDQQLNPSSQGPADAFSTQEQCTMCGVPIGESRVDFPADSRESESWRDAGSSSRIDEIQTPVGLRRRKGSHLVESRDNQRAASRTANRDGTCFWWNENSDLYLLLLLWVLLYCLMLLPQMDLKELPSLLLNLD

Target ORF information:

RefSeq Version	XM_023951389.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes calmin (clmn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023951389.1

ORF Insert Sequence:

ATGGCTAGAC ACGAGCGGCA GGACTGGTTC GAGCGCGAGG AATTCATCGG ACAGATCAGC 
GACATACGCG TCCAAAACCT GCAAGTGGAA AGGGAGGCGG TGCAGAAGAG GACCTTCACT 
CGATGGATGA ACCTCCACTT ACAAAAGTGC GACCCGCCCA TCCAGATCCA AGACCTGTTC 
CAGGACATCC AGGATGGGTT TATCCTCATG GTTCTGCTGG AGGAACTCTC CGGCTGCAAA 
CTGATTCAAG GCTTCAAGAA GTCCTCCCAC CGAATTTTCC GACTCAACAA CATTGCCAAA 
GTCTTGGCCT TCCTGGAGGA GCGGAACGTG AAGCTGGTCA GCATTGATGC AGCTGACGTT 
GCTGATGGGA ACTCGTCCAT CATCCTTGGA CTCCTCTGGA ACATCATCCT TTTCTTTCAG 
ATTAAAGAGC TAACTGGAAA CATTCAGAGT CAGTTTCCCT CCTGTTCCAG CCTTTCGTCC 
ATCCCTACGA GCTCTGACTC GGACACGTCC CACAGCAGCA CGCCGTCCGA GGAAAGACGG 
AGCGCCAGCA ACGCTCTCAA GGAGAACAGC AAAGCCATCA AAAAGCTGCT GCAGTGGATT 
CAGAGGAAAA CCCGCAAGTA TGGCGTGGCG GTTCAAGATT TTGGAAGAAG CTGGACAAGT 
GGATTGGCCT TCCTGGCCCT CATTAAGTCC ATTGATTCCA GCCTGGTAGA CATGAGGAAA 
GCCCTACTGA GGAGTTCAAG AGAGAATCTG GAGGATGCCT TCAGGATAGC TCACTACAGC 
CTGGGCATTC CACGCCTGCT GGAGCCCGAG GATGTGGGTT TTAATGAGCC AGACGAGCAG 
TCCATCATCA TGTATGTTTC CCAGTTCTTG GAGCATTTCC CTGGACTTGA GGAGGCGGAG 
GAGTCCACTC AGCTGATTGA GCGGAGCGTC TCCATGGGCC GACTCAACGA CGCAGATCTG 
ATGAGAAACG GCGCCAATCT CAGCAGAGTG AAAGAGCGGT CCAGATTGTT CCAGAGAGAC 
GGCTATAAGC CGCCTCCCAA AGTCCTACTT TCTTCCGTGT CGGAGGACAG AGGCATCCTT 
TCGCCTCGCC TCAGGGTGGC TGCAACTCGC TCCTGGTCCA GTGAAGACGT TCTGTCTGAC 
TCGCCTCTTG ACGTTGTCTC TAATGACGCC CAAGGAGTCG CTGAGGTCCA GCAGGATTTG 
ACCTGCACCT TACCTCAGCA GTCTTTCTCT CATTCACCGT CATTCGCACC GGATTGTGGA 
AACCCAGAGC CTGTAATCCC CGACTCTGCC ATCCACTCCC CAGAATCCTG GATGGAGAGT 
GAGCTGATGC TTGAGACTCA CAGTGACAGT TCTCTGTTTG ACAGCGGGCC GGGCTGGGAT 
GTGTACCGGG CCACACCTGT GCAGGTGACA CCAGTGGATG AAGGATTCAT GTTGTCCATG 
GACGACAGAG CTCCAGATGA ACCCTTTGTG ACGGGATCAT ACAGCGATGA AGGAGTCTCC 
TCGTTGGAGA GCACCCAGGA CAAAGCCCAC CAGGAGGAAC AGGACAAGGA GCTGAACTCA 
AAGAACCAGA ATCTGGTTCA GGAACTCATC ATTGATCCAA GTGAAGTTGA CTTTTCAAAA 
GAGACAGACC TCAGTCCCAT CAGTACAAGT CCACTCCACA AAGACAGTGA GCAGGAACGG 
TCCTGGACAT TCAATGAAGA ATTAACTCTC TCAGGTTCTG AAGATTTCCT TAAAGATGGT 
CAAACAAACC ATTTTCAACC AGCCTTCTCC ACAGATGTTC CACCTCATGA AGAACACACT 
GAAAGTTCTC AGAGAGAACA GGAAGGGAAC AAAAAGAACC TAAATGAACA AACTGATGGT 
CCATCTGGGG AACTACAGGA GAGGGGAACC TATGTGGAAG AGTATCAAAA AGCTGACTCT 
TCAGTGGAAG ATGAAGGAAT CGAGGTAAAA GGTGGAAATG ATCAAGACCA GAAAACCCAG 
GAGGAAGAAC TCTTAGCTAA CGCCTCACAT GATGAAGAAA TCAGTAGTTC TCAGATGATA 
TTAACTAATC AAGAGGGAGC ATCAAAAACT GGTCTAATTA TTCCCATCAT TTCCATCTCC 
AGTGAGCCAA AGGAGCACAA AGAAGAATCT GGCTCAGAAG ACCAGGAAAC AGAGGAGAAA 
CTCCATCAGC AGTTTGAGTC TGGATATGGG GAAGATGACA CGGAATTGAA CAGTCCTACA 
AGTCCAGTTG CTCTGGAGCA TGGTCGTCTG GAGGATGGTC TTTTGGAGTG TGTTCCTCTG 
GAGCACAGTC CTCTAGAGCA TGTCTACAGT GAGGACCTCA AAGAAGAATC TGAGGAGAGA 
CCTTCCTGTC AGATATCAGA AAATTCTGAA AGGAGGCCTT TGCAAACCAC CCACTCTGAA 
GACAAACTAC TGTCTGATGG AGATGAACCT CCCAGAAACT CTGGGTCAGA ATGTGTGCAG 
AACCAGGAAA TGGATGAAGC CAGACAAAAG CACAGCTCAG ATGATTTTGC AGCTGTAAAG 
AAGACAATGG CTCAGGCAAA TCCTGACAGT GATGCAAGTC AGGAACCTGA GGGACATGGA 
GTAAAACCAG CCGGTCCTGC CTCTGAAAGC GGCCCCCAAA TCCCCCATCC GAGCATACTC 
GACACAGACA CTTTAGACCT AAACGAAGCA AAAGTTTCTC CATTTTCTGG ACCACCAGGA 
TTAAACAGAA ACATGGACTT GTTCTATTCC AAATTTGACA AAAGCTCTCC CTCAGAGCAT 
CTAGTTGGTG ACCCAGTGGA ACCCATGGAT CTGTTCTATC CTGACAAAGA GGAGCCCATG 
CTCTCAGAAC CTGCTGACAC GGAGATGCAG AGGTGGCCTT CAGTTCTGAG CGTCTCAGCT 
CTGGAGCCAG CGCCGCCGTC ACTCGATGAC ATGGATGACG AACAGCTGAC GGGAGAGGAG 
GATGATGATC AACAGCTGAA TCCATCCAGC CAGGGGCCTG CTGATGCCTT CTCCACCCAG 
GAGCAGTGCA CGATGTGCGG GGTGCCGATT GGGGAATCCC GGGTGGATTT TCCAGCAGAC 
AGCAGGGAGT CTGAGAGTTG GAGAGATGCG GGAAGTTCCA GCAGAATAGA CGAGATCCAG 
ACCCCTGTAG GTCTCCGGCG CAGGAAGGGT TCTCACCTCG TGGAGTCCAG GGACAACCAG 
AGAGCTGCAT CAAGAACAGC CAACAGAGAT GGCACTTGCT TTTGGTGGAA CGAGAATTCG 
GACCTCTATC TGCTGCTGCT GCTCTGGGTG CTTCTGTACT GCCTCATGCT GCTGCCGCAG 
ATGGACCTAA AGGAGCTGCC GAGTCTTCTG CTGAACCTCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

clmn ( XM_023951390.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023807158.1 Oryzias latipes calmin (clmn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OJa113602
Clone ID Related Accession (Same CDS sequence)	XM_023951390.1
Accession Version	XM_023951390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	calmin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGAC ACGAGCGGCA GGACTGGTTC GAGCGCGAGG AATTCATCGG ACAGATCAGC 
GACATACGCG TCCAAAACCT GCAAGTGGAA AGGGAGGCGG TGCAGAAGAG GACCTTCACT 
CGATGGATGA ACCTCCACTT ACAAAAGTGC GACCCGCCCA TCCAGATCCA AGACCTGTTC 
CAGGACATCC AGGATGGGTT TATCCTCATG GTTCTGCTGG AGGAACTCTC CGGCTGCAAA 
CTGATTCAAG GCTTCAAGAA GTCCTCCCAC CGAATTTTCC GACTCAACAA CATTGCCAAA 
GTCTTGGCCT TCCTGGAGGA GCGGAACGTG AAGCTGGTCA GCATTGATGC AGCTGACGTT 
GCTGATGGGA ACTCGTCCAT CATCCTTGGA CTCCTCTGGA ACATCATCCT TTTCTTTCAG 
ATTAAAGAGC TAACTGGAAA CATTCAGAGT CAGTTTCCCT CCTGTTCCAG CCTTTCGTCC 
ATCCCTACGA GCTCTGACTC GGACACGTCC CACAGCAGCA CGCCGTCCGA GGAAAGACGG 
AGCGCCAGCA ACGCTCTCAA GGAGAACAGC AAAGCCATCA AAAAGCTGCT GCAGTGGATT 
CAGAGGAAAA CCCGCAAGTA TGGCGTGGCG GTTCAAGATT TTGGAAGAAG CTGGACAAGT 
GGATTGGCCT TCCTGGCCCT CATTAAGTCC ATTGATTCCA GCCTGGTAGA CATGAGGAAA 
GCCCTACTGA GGAGTTCAAG AGAGAATCTG GAGGATGCCT TCAGGATAGC TCACTACAGC 
CTGGGCATTC CACGCCTGCT GGAGCCCGAG GATGTGGGTT TTAATGAGCC AGACGAGCAG 
TCCATCATCA TGTATGTTTC CCAGTTCTTG GAGCATTTCC CTGGACTTGA GGAGGCGGAG 
GAGTCCACTC AGCTGATTGA GCGGAGCGTC TCCATGGGCC GACTCAACGA CGCAGATCTG 
ATGAGAAACG GCGCCAATCT CAGCAGAGTG AAAGAGCGGT CCAGATTGTT CCAGAGAGAC 
GGCTATAAGC CGCCTCCCAA AGTCCTACTT TCTTCCGTGT CGGAGGACAG AGGCATCCTT 
TCGCCTCGCC TCAGGGTGGC TGCAACTCGC TCCTGGTCCA GTGAAGACGT TCTGTCTGAC 
TCGCCTCTTG ACGTTGTCTC TAATGACGCC CAAGGAGTCG CTGAGGTCCA GCAGGATTTG 
ACCTGCACCT TACCTCAGCA GTCTTTCTCT CATTCACCGT CATTCGCACC GGATTGTGGA 
AACCCAGAGC CTGTAATCCC CGACTCTGCC ATCCACTCCC CAGAATCCTG GATGGAGAGT 
GAGCTGATGC TTGAGACTCA CAGTGACAGT TCTCTGTTTG ACAGCGGGCC GGGCTGGGAT 
GTGTACCGGG CCACACCTGT GCAGGTGACA CCAGTGGATG AAGGATTCAT GTTGTCCATG 
GACGACAGAG CTCCAGATGA ACCCTTTGTG ACGGGATCAT ACAGCGATGA AGGAGTCTCC 
TCGTTGGAGA GCACCCAGGA CAAAGCCCAC CAGGAGGAAC AGGACAAGGA GCTGAACTCA 
AAGAACCAGA ATCTGGTTCA GGAACTCATC ATTGATCCAA GTGAAGTTGA CTTTTCAAAA 
GAGACAGACC TCAGTCCCAT CAGTACAAGT CCACTCCACA AAGACAGTGA GCAGGAACGG 
TCCTGGACAT TCAATGAAGA ATTAACTCTC TCAGGTTCTG AAGATTTCCT TAAAGATGGT 
CAAACAAACC ATTTTCAACC AGCCTTCTCC ACAGATGTTC CACCTCATGA AGAACACACT 
GAAAGTTCTC AGAGAGAACA GGAAGGGAAC AAAAAGAACC TAAATGAACA AACTGATGGT 
CCATCTGGGG AACTACAGGA GAGGGGAACC TATGTGGAAG AGTATCAAAA AGCTGACTCT 
TCAGTGGAAG ATGAAGGAAT CGAGGTAAAA GGTGGAAATG ATCAAGACCA GAAAACCCAG 
GAGGAAGAAC TCTTAGCTAA CGCCTCACAT GATGAAGAAA TCAGTAGTTC TCAGATGATA 
TTAACTAATC AAGAGGGAGC ATCAAAAACT GGTCTAATTA TTCCCATCAT TTCCATCTCC 
AGTGAGCCAA AGGAGCACAA AGAAGAATCT GGCTCAGAAG ACCAGGAAAC AGAGGAGAAA 
CTCCATCAGC AGTTTGAGTC TGGATATGGG GAAGATGACA CGGAATTGAA CAGTCCTACA 
AGTCCAGTTG CTCTGGAGCA TGGTCGTCTG GAGGATGGTC TTTTGGAGTG TGTTCCTCTG 
GAGCACAGTC CTCTAGAGCA TGTCTACAGT GAGGACCTCA AAGAAGAATC TGAGGAGAGA 
CCTTCCTGTC AGATATCAGA AAATTCTGAA AGGAGGCCTT TGCAAACCAC CCACTCTGAA 
GACAAACTAC TGTCTGATGG AGATGAACCT CCCAGAAACT CTGGGTCAGA ATGTGTGCAG 
AACCAGGAAA TGGATGAAGC CAGACAAAAG CACAGCTCAG ATGATTTTGC AGCTGTAAAG 
AAGACAATGG CTCAGGCAAA TCCTGACAGT GATGCAAGTC AGGAACCTGA GGGACATGGA 
GTAAAACCAG CCGGTCCTGC CTCTGAAAGC GGCCCCCAAA TCCCCCATCC GAGCATACTC 
GACACAGACA CTTTAGACCT AAACGAAGCA AAAGTTTCTC CATTTTCTGG ACCACCAGGA 
TTAAACAGAA ACATGGACTT GTTCTATTCC AAATTTGACA AAAGCTCTCC CTCAGAGCAT 
CTAGTTGGTG ACCCAGTGGA ACCCATGGAT CTGTTCTATC CTGACAAAGA GGAGCCCATG 
CTCTCAGAAC CTGCTGACAC GGAGATGCAG AGGTGGCCTT CAGTTCTGAG CGTCTCAGCT 
CTGGAGCCAG CGCCGCCGTC ACTCGATGAC ATGGATGACG AACAGCTGAC GGGAGAGGAG 
GATGATGATC AACTGAATCC ATCCAGCCAG GGGCCTGCTG ATGCCTTCTC CACCCAGGAG 
CAGTGCACGA TGTGCGGGGT GCCGATTGGG GAATCCCGGG TGGATTTTCC AGCAGACAGC 
AGGGAGTCTG AGAGTTGGAG AGATGCGGGA AGTTCCAGCA GAATAGACGA GATCCAGACC 
CCTGTAGGTC TCCGGCGCAG GAAGGGTTCT CACCTCGTGG AGTCCAGGGA CAACCAGAGA 
GCTGCATCAA GAACAGCCAA CAGAGATGGC ACTTGCTTTT GGTGGAACGA GAATTCGGAC 
CTCTATCTGC TGCTGCTGCT CTGGGTGCTT CTGTACTGCC TCATGCTGCT GCCGCAGATG 
GACCTAAAGG AGCTGCCGAG TCTTCTGCTG AACCTCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023807158.1
CDS	360..3701
Translation	MARHERQDWFEREEFIGQISDIRVQNLQVEREAVQKRTFTRWMNLHLQKCDPPIQIQDLFQDIQDGFILMVLLEELSGCKLIQGFKKSSHRIFRLNNIAKVLAFLEERNVKLVSIDAADVADGNSSIILGLLWNIILFFQIKELTGNIQSQFPSCSSLSSIPTSSDSDTSHSSTPSEERRSASNALKENSKAIKKLLQWIQRKTRKYGVAVQDFGRSWTSGLAFLALIKSIDSSLVDMRKALLRSSRENLEDAFRIAHYSLGIPRLLEPEDVGFNEPDEQSIIMYVSQFLEHFPGLEEAEESTQLIERSVSMGRLNDADLMRNGANLSRVKERSRLFQRDGYKPPPKVLLSSVSEDRGILSPRLRVAATRSWSSEDVLSDSPLDVVSNDAQGVAEVQQDLTCTLPQQSFSHSPSFAPDCGNPEPVIPDSAIHSPESWMESELMLETHSDSSLFDSGPGWDVYRATPVQVTPVDEGFMLSMDDRAPDEPFVTGSYSDEGVSSLESTQDKAHQEEQDKELNSKNQNLVQELIIDPSEVDFSKETDLSPISTSPLHKDSEQERSWTFNEELTLSGSEDFLKDGQTNHFQPAFSTDVPPHEEHTESSQREQEGNKKNLNEQTDGPSGELQERGTYVEEYQKADSSVEDEGIEVKGGNDQDQKTQEEELLANASHDEEISSSQMILTNQEGASKTGLIIPIISISSEPKEHKEESGSEDQETEEKLHQQFESGYGEDDTELNSPTSPVALEHGRLEDGLLECVPLEHSPLEHVYSEDLKEESEERPSCQISENSERRPLQTTHSEDKLLSDGDEPPRNSGSECVQNQEMDEARQKHSSDDFAAVKKTMAQANPDSDASQEPEGHGVKPAGPASESGPQIPHPSILDTDTLDLNEAKVSPFSGPPGLNRNMDLFYSKFDKSSPSEHLVGDPVEPMDLFYPDKEEPMLSEPADTEMQRWPSVLSVSALEPAPPSLDDMDDEQLTGEEDDDQLNPSSQGPADAFSTQEQCTMCGVPIGESRVDFPADSRESESWRDAGSSSRIDEIQTPVGLRRRKGSHLVESRDNQRAASRTANRDGTCFWWNENSDLYLLLLLWVLLYCLMLLPQMDLKELPSLLLNLD

Target ORF information:

RefSeq Version	XM_023951390.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes calmin (clmn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023951390.1

ORF Insert Sequence:

ATGGCTAGAC ACGAGCGGCA GGACTGGTTC GAGCGCGAGG AATTCATCGG ACAGATCAGC 
GACATACGCG TCCAAAACCT GCAAGTGGAA AGGGAGGCGG TGCAGAAGAG GACCTTCACT 
CGATGGATGA ACCTCCACTT ACAAAAGTGC GACCCGCCCA TCCAGATCCA AGACCTGTTC 
CAGGACATCC AGGATGGGTT TATCCTCATG GTTCTGCTGG AGGAACTCTC CGGCTGCAAA 
CTGATTCAAG GCTTCAAGAA GTCCTCCCAC CGAATTTTCC GACTCAACAA CATTGCCAAA 
GTCTTGGCCT TCCTGGAGGA GCGGAACGTG AAGCTGGTCA GCATTGATGC AGCTGACGTT 
GCTGATGGGA ACTCGTCCAT CATCCTTGGA CTCCTCTGGA ACATCATCCT TTTCTTTCAG 
ATTAAAGAGC TAACTGGAAA CATTCAGAGT CAGTTTCCCT CCTGTTCCAG CCTTTCGTCC 
ATCCCTACGA GCTCTGACTC GGACACGTCC CACAGCAGCA CGCCGTCCGA GGAAAGACGG 
AGCGCCAGCA ACGCTCTCAA GGAGAACAGC AAAGCCATCA AAAAGCTGCT GCAGTGGATT 
CAGAGGAAAA CCCGCAAGTA TGGCGTGGCG GTTCAAGATT TTGGAAGAAG CTGGACAAGT 
GGATTGGCCT TCCTGGCCCT CATTAAGTCC ATTGATTCCA GCCTGGTAGA CATGAGGAAA 
GCCCTACTGA GGAGTTCAAG AGAGAATCTG GAGGATGCCT TCAGGATAGC TCACTACAGC 
CTGGGCATTC CACGCCTGCT GGAGCCCGAG GATGTGGGTT TTAATGAGCC AGACGAGCAG 
TCCATCATCA TGTATGTTTC CCAGTTCTTG GAGCATTTCC CTGGACTTGA GGAGGCGGAG 
GAGTCCACTC AGCTGATTGA GCGGAGCGTC TCCATGGGCC GACTCAACGA CGCAGATCTG 
ATGAGAAACG GCGCCAATCT CAGCAGAGTG AAAGAGCGGT CCAGATTGTT CCAGAGAGAC 
GGCTATAAGC CGCCTCCCAA AGTCCTACTT TCTTCCGTGT CGGAGGACAG AGGCATCCTT 
TCGCCTCGCC TCAGGGTGGC TGCAACTCGC TCCTGGTCCA GTGAAGACGT TCTGTCTGAC 
TCGCCTCTTG ACGTTGTCTC TAATGACGCC CAAGGAGTCG CTGAGGTCCA GCAGGATTTG 
ACCTGCACCT TACCTCAGCA GTCTTTCTCT CATTCACCGT CATTCGCACC GGATTGTGGA 
AACCCAGAGC CTGTAATCCC CGACTCTGCC ATCCACTCCC CAGAATCCTG GATGGAGAGT 
GAGCTGATGC TTGAGACTCA CAGTGACAGT TCTCTGTTTG ACAGCGGGCC GGGCTGGGAT 
GTGTACCGGG CCACACCTGT GCAGGTGACA CCAGTGGATG AAGGATTCAT GTTGTCCATG 
GACGACAGAG CTCCAGATGA ACCCTTTGTG ACGGGATCAT ACAGCGATGA AGGAGTCTCC 
TCGTTGGAGA GCACCCAGGA CAAAGCCCAC CAGGAGGAAC AGGACAAGGA GCTGAACTCA 
AAGAACCAGA ATCTGGTTCA GGAACTCATC ATTGATCCAA GTGAAGTTGA CTTTTCAAAA 
GAGACAGACC TCAGTCCCAT CAGTACAAGT CCACTCCACA AAGACAGTGA GCAGGAACGG 
TCCTGGACAT TCAATGAAGA ATTAACTCTC TCAGGTTCTG AAGATTTCCT TAAAGATGGT 
CAAACAAACC ATTTTCAACC AGCCTTCTCC ACAGATGTTC CACCTCATGA AGAACACACT 
GAAAGTTCTC AGAGAGAACA GGAAGGGAAC AAAAAGAACC TAAATGAACA AACTGATGGT 
CCATCTGGGG AACTACAGGA GAGGGGAACC TATGTGGAAG AGTATCAAAA AGCTGACTCT 
TCAGTGGAAG ATGAAGGAAT CGAGGTAAAA GGTGGAAATG ATCAAGACCA GAAAACCCAG 
GAGGAAGAAC TCTTAGCTAA CGCCTCACAT GATGAAGAAA TCAGTAGTTC TCAGATGATA 
TTAACTAATC AAGAGGGAGC ATCAAAAACT GGTCTAATTA TTCCCATCAT TTCCATCTCC 
AGTGAGCCAA AGGAGCACAA AGAAGAATCT GGCTCAGAAG ACCAGGAAAC AGAGGAGAAA 
CTCCATCAGC AGTTTGAGTC TGGATATGGG GAAGATGACA CGGAATTGAA CAGTCCTACA 
AGTCCAGTTG CTCTGGAGCA TGGTCGTCTG GAGGATGGTC TTTTGGAGTG TGTTCCTCTG 
GAGCACAGTC CTCTAGAGCA TGTCTACAGT GAGGACCTCA AAGAAGAATC TGAGGAGAGA 
CCTTCCTGTC AGATATCAGA AAATTCTGAA AGGAGGCCTT TGCAAACCAC CCACTCTGAA 
GACAAACTAC TGTCTGATGG AGATGAACCT CCCAGAAACT CTGGGTCAGA ATGTGTGCAG 
AACCAGGAAA TGGATGAAGC CAGACAAAAG CACAGCTCAG ATGATTTTGC AGCTGTAAAG 
AAGACAATGG CTCAGGCAAA TCCTGACAGT GATGCAAGTC AGGAACCTGA GGGACATGGA 
GTAAAACCAG CCGGTCCTGC CTCTGAAAGC GGCCCCCAAA TCCCCCATCC GAGCATACTC 
GACACAGACA CTTTAGACCT AAACGAAGCA AAAGTTTCTC CATTTTCTGG ACCACCAGGA 
TTAAACAGAA ACATGGACTT GTTCTATTCC AAATTTGACA AAAGCTCTCC CTCAGAGCAT 
CTAGTTGGTG ACCCAGTGGA ACCCATGGAT CTGTTCTATC CTGACAAAGA GGAGCCCATG 
CTCTCAGAAC CTGCTGACAC GGAGATGCAG AGGTGGCCTT CAGTTCTGAG CGTCTCAGCT 
CTGGAGCCAG CGCCGCCGTC ACTCGATGAC ATGGATGACG AACAGCTGAC GGGAGAGGAG 
GATGATGATC AACTGAATCC ATCCAGCCAG GGGCCTGCTG ATGCCTTCTC CACCCAGGAG 
CAGTGCACGA TGTGCGGGGT GCCGATTGGG GAATCCCGGG TGGATTTTCC AGCAGACAGC 
AGGGAGTCTG AGAGTTGGAG AGATGCGGGA AGTTCCAGCA GAATAGACGA GATCCAGACC 
CCTGTAGGTC TCCGGCGCAG GAAGGGTTCT CACCTCGTGG AGTCCAGGGA CAACCAGAGA 
GCTGCATCAA GAACAGCCAA CAGAGATGGC ACTTGCTTTT GGTGGAACGA GAATTCGGAC 
CTCTATCTGC TGCTGCTGCT CTGGGTGCTT CTGTACTGCC TCATGCTGCT GCCGCAGATG 
GACCTAAAGG AGCTGCCGAG TCTTCTGCTG AACCTCGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

clmn ( XM_011490151.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011488453.2 Oryzias latipes calmin (clmn), mRNA.

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CloneID	OJa75646
Clone ID Related Accession (Same CDS sequence)	XM_011490151.2
Accession Version	XM_011490151.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	calmin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011490151.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACATA CCTGGAGCAG CCCAGAGTGC GCAGTCCTGT CCCCCCACCC CTCTGCACAA 
ATGGAAAGGG AGGCGGTGCA GAAGAGGACC TTCACTCGAT GGATGAACCT CCACTTACAA 
AAGTGCGACC CGCCCATCCA GATCCAAGAC CTGTTCCAGG ACATCCAGGA TGGGTTTATC 
CTCATGGTTC TGCTGGAGGA ACTCTCCGGC TGCAAACTGA TTCAAGGCTT CAAGAAGTCC 
TCCCACCGAA TTTTCCGACT CAACAACATT GCCAAAGTCT TGGCCTTCCT GGAGGAGCGG 
AACGTGAAGC TGGTCAGCAT TGATGCAGCT GACGTTGCTG ATGGGAACTC GTCCATCATC 
CTTGGACTCC TCTGGAACAT CATCCTTTTC TTTCAGATTA AAGAGCTAAC TGGAAACATT 
CAGAGTCAGT TTCCCTCCTG TTCCAGCCTT TCGTCCATCC CTACGAGCTC TGACTCGGAC 
ACGTCCCACA GCAGCACGCC GTCCGAGGAA AGACGGAGCG CCAGCAACGC TCTCAAGGAG 
AACAGCAAAG CCATCAAAAA GCTGCTGCAG TGGATTCAGA GGAAAACCCG CAAGTATGGC 
GTGGCGGTTC AAGATTTTGG AAGAAGCTGG ACAAGTGGAT TGGCCTTCCT GGCCCTCATT 
AAGTCCATTG ATTCCAGCCT GGTAGACATG AGGAAAGCCC TACTGAGGAG TTCAAGAGAG 
AATCTGGAGG ATGCCTTCAG GATAGCTCAC TACAGCCTGG GCATTCCACG CCTGCTGGAG 
CCCGAGGATG TGGGTTTTAA TGAGCCAGAC GAGCAGTCCA TCATCATGTA TGTTTCCCAG 
TTCTTGGAGC ATTTCCCTGG ACTTGAGGAG GCGGAGGAGT CCACTCAGCT GATTGAGCGG 
AGCGTCTCCA TGGGCCGACT CAACGACGCA GATCTGATGA GAAACGGCGC CAATCTCAGC 
AGAGTGAAAG AGCGGTCCAG ATTGTTCCAG AGAGACGGCT ATAAGCCGCC TCCCAAAGTC 
CTACTTTCTT CCGTGTCGGA GGACAGAGGC ATCCTTTCGC CTCGCCTCAG GGTGGCTGCA 
ACTCGCTCCT GGTCCAGTGA AGACGTTCTG TCTGACTCGC CTCTTGACGT TGTCTCTAAT 
GACGCCCAAG GAGTCGCTGA GGTCCAGCAG GATTTGACCT GCACCTTACC TCAGCAGTCT 
TTCTCTCATT CACCGTCATT CGCACCGGAT TGTGGAAACC CAGAGCCTGT AATCCCCGAC 
TCTGCCATCC ACTCCCCAGA ATCCTGGATG GAGAGTGAGC TGATGCTTGA GACTCACAGT 
GACAGTTCTC TGTTTGACAG CGGGCCGGGC TGGGATGTGT ACCGGGCCAC ACCTGTGCAG 
GTGACACCAG TGGATGAAGG ATTCATGTTG TCCATGGACG ACAGAGCTCC AGATGAACCC 
TTTGTGACGG GATCATACAG CGATGAAGGA GTCTCCTCGT TGGAGAGCAC CCAGGACAAA 
GCCCACCAGG AGGAACAGGA CAAGGAGCTG AACTCAAAGA ACCAGAATCT GGTTCAGGAA 
CTCATCATTG ATCCAAGTGA AGTTGACTTT TCAAAAGAGA CAGACCTCAG TCCCATCAGT 
ACAAGTCCAC TCCACAAAGA CAGTGAGCAG GAACGGTCCT GGACATTCAA TGAAGAATTA 
ACTCTCTCAG GTTCTGAAGA TTTCCTTAAA GATGGTCAAA CAAACCATTT TCAACCAGCC 
TTCTCCACAG ATGTTCCACC TCATGAAGAA CACACTGAAA GTTCTCAGAG AGAACAGGAA 
GGGAACAAAA AGAACCTAAA TGAACAAACT GATGGTCCAT CTGGGGAACT ACAGGAGAGG 
GGAACCTATG TGGAAGAGTA TCAAAAAGCT GACTCTTCAG TGGAAGATGA AGGAATCGAG 
GTAAAAGGTG GAAATGATCA AGACCAGAAA ACCCAGGAGG AAGAACTCTT AGCTAACGCC 
TCACATGATG AAGAAATCAG TAGTTCTCAG ATGATATTAA CTAATCAAGA GGGAGCATCA 
AAAACTGGTC TAATTATTCC CATCATTTCC ATCTCCAGTG AGCCAAAGGA GCACAAAGAA 
GAATCTGGCT CAGAAGACCA GGAAACAGAG GAGAAACTCC ATCAGCAGTT TGAGTCTGGA 
TATGGGGAAG ATGACACGGA ATTGAACAGT CCTACAAGTC CAGTTGCTCT GGAGCATGGT 
CGTCTGGAGG ATGGTCTTTT GGAGTGTGTT CCTCTGGAGC ACAGTCCTCT AGAGCATGTC 
TACAGTGAGG ACCTCAAAGA AGAATCTGAG GAGAGACCTT CCTGTCAGAT ATCAGAAAAT 
TCTGAAAGGA GGCCTTTGCA AACCACCCAC TCTGAAGACA AACTACTGTC TGATGGAGAT 
GAACCTCCCA GAAACTCTGG GTCAGAATGT GTGCAGAACC AGGAAATGGA TGAAGCCAGA 
CAAAAGCACA GCTCAGATGA TTTTGCAGCT GTAAAGAAGA CAATGGCTCA GGCAAATCCT 
GACAGTGATG CAAGTCAGGA ACCTGAGGGA CATGGAGTAA AACCAGCCGG TCCTGCCTCT 
GAAAGCGGCC CCCAAATCCC CCATCCGAGC ATACTCGACA CAGACACTTT AGACCTAAAC 
GAAGCAAAAG TTTCTCCATT TTCTGGACCA CCAGGATTAA ACAGAAACAT GGACTTGTTC 
TATTCCAAAT TTGACAAAAG CTCTCCCTCA GAGCATCTAG TTGGTGACCC AGTGGAACCC 
ATGGATCTGT TCTATCCTGA CAAAGAGGAG CCCATGCTCT CAGAACCTGC TGACACGGAG 
ATGCAGAGGT GGCCTTCAGT TCTGAGCGTC TCAGCTCTGG AGCCAGCGCC GCCGTCACTC 
GATGACATGG ATGACGAACA GCTGACGGGA GAGGAGGATG ATGATCAACA GCTGAATCCA 
TCCAGCCAGG GGCCTGCTGA TGCCTTCTCC ACCCAGGAGC AGTGCACGAT GTGCGGGGTG 
CCGATTGGGG AATCCCGGGT GGATTTTCCA GCAGACAGCA GGGAGTCTGA GAGTTGGAGA 
GATGCGGGAA GTTCCAGCAG AATAGACGAG ATCCAGACCC CTGTAGGTCT CCGGCGCAGG 
AAGGGTTCTC ACCTCGTGGA GTCCAGGGAC AACCAGAGAG CTGCATCAAG AACAGCCAAC 
AGAGATGGCA CTTGCTTTTG GTGGAACGAG AATTCGGACC TCTATCTGCT GCTGCTGCTC 
TGGGTGCTTC TGTACTGCCT CATGCTGCTG CCGCAGATGG ACCTAAAGGA GCTGCCGAGT 
CTTCTGCTGA ACCTCGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011488453.2
CDS	62..3382
Translation	MEHTWSSPECAVLSPHPSAQMEREAVQKRTFTRWMNLHLQKCDPPIQIQDLFQDIQDGFILMVLLEELSGCKLIQGFKKSSHRIFRLNNIAKVLAFLEERNVKLVSIDAADVADGNSSIILGLLWNIILFFQIKELTGNIQSQFPSCSSLSSIPTSSDSDTSHSSTPSEERRSASNALKENSKAIKKLLQWIQRKTRKYGVAVQDFGRSWTSGLAFLALIKSIDSSLVDMRKALLRSSRENLEDAFRIAHYSLGIPRLLEPEDVGFNEPDEQSIIMYVSQFLEHFPGLEEAEESTQLIERSVSMGRLNDADLMRNGANLSRVKERSRLFQRDGYKPPPKVLLSSVSEDRGILSPRLRVAATRSWSSEDVLSDSPLDVVSNDAQGVAEVQQDLTCTLPQQSFSHSPSFAPDCGNPEPVIPDSAIHSPESWMESELMLETHSDSSLFDSGPGWDVYRATPVQVTPVDEGFMLSMDDRAPDEPFVTGSYSDEGVSSLESTQDKAHQEEQDKELNSKNQNLVQELIIDPSEVDFSKETDLSPISTSPLHKDSEQERSWTFNEELTLSGSEDFLKDGQTNHFQPAFSTDVPPHEEHTESSQREQEGNKKNLNEQTDGPSGELQERGTYVEEYQKADSSVEDEGIEVKGGNDQDQKTQEEELLANASHDEEISSSQMILTNQEGASKTGLIIPIISISSEPKEHKEESGSEDQETEEKLHQQFESGYGEDDTELNSPTSPVALEHGRLEDGLLECVPLEHSPLEHVYSEDLKEESEERPSCQISENSERRPLQTTHSEDKLLSDGDEPPRNSGSECVQNQEMDEARQKHSSDDFAAVKKTMAQANPDSDASQEPEGHGVKPAGPASESGPQIPHPSILDTDTLDLNEAKVSPFSGPPGLNRNMDLFYSKFDKSSPSEHLVGDPVEPMDLFYPDKEEPMLSEPADTEMQRWPSVLSVSALEPAPPSLDDMDDEQLTGEEDDDQQLNPSSQGPADAFSTQEQCTMCGVPIGESRVDFPADSRESESWRDAGSSSRIDEIQTPVGLRRRKGSHLVESRDNQRAASRTANRDGTCFWWNENSDLYLLLLLWVLLYCLMLLPQMDLKELPSLLLNLD

Target ORF information:

RefSeq Version	XM_011490151.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes calmin (clmn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011490151.2

ORF Insert Sequence:

ATGGAACATA CCTGGAGCAG CCCAGAGTGC GCAGTCCTGT CCCCCCACCC CTCTGCACAA 
ATGGAAAGGG AGGCGGTGCA GAAGAGGACC TTCACTCGAT GGATGAACCT CCACTTACAA 
AAGTGCGACC CGCCCATCCA GATCCAAGAC CTGTTCCAGG ACATCCAGGA TGGGTTTATC 
CTCATGGTTC TGCTGGAGGA ACTCTCCGGC TGCAAACTGA TTCAAGGCTT CAAGAAGTCC 
TCCCACCGAA TTTTCCGACT CAACAACATT GCCAAAGTCT TGGCCTTCCT GGAGGAGCGG 
AACGTGAAGC TGGTCAGCAT TGATGCAGCT GACGTTGCTG ATGGGAACTC GTCCATCATC 
CTTGGACTCC TCTGGAACAT CATCCTTTTC TTTCAGATTA AAGAGCTAAC TGGAAACATT 
CAGAGTCAGT TTCCCTCCTG TTCCAGCCTT TCGTCCATCC CTACGAGCTC TGACTCGGAC 
ACGTCCCACA GCAGCACGCC GTCCGAGGAA AGACGGAGCG CCAGCAACGC TCTCAAGGAG 
AACAGCAAAG CCATCAAAAA GCTGCTGCAG TGGATTCAGA GGAAAACCCG CAAGTATGGC 
GTGGCGGTTC AAGATTTTGG AAGAAGCTGG ACAAGTGGAT TGGCCTTCCT GGCCCTCATT 
AAGTCCATTG ATTCCAGCCT GGTAGACATG AGGAAAGCCC TACTGAGGAG TTCAAGAGAG 
AATCTGGAGG ATGCCTTCAG GATAGCTCAC TACAGCCTGG GCATTCCACG CCTGCTGGAG 
CCCGAGGATG TGGGTTTTAA TGAGCCAGAC GAGCAGTCCA TCATCATGTA TGTTTCCCAG 
TTCTTGGAGC ATTTCCCTGG ACTTGAGGAG GCGGAGGAGT CCACTCAGCT GATTGAGCGG 
AGCGTCTCCA TGGGCCGACT CAACGACGCA GATCTGATGA GAAACGGCGC CAATCTCAGC 
AGAGTGAAAG AGCGGTCCAG ATTGTTCCAG AGAGACGGCT ATAAGCCGCC TCCCAAAGTC 
CTACTTTCTT CCGTGTCGGA GGACAGAGGC ATCCTTTCGC CTCGCCTCAG GGTGGCTGCA 
ACTCGCTCCT GGTCCAGTGA AGACGTTCTG TCTGACTCGC CTCTTGACGT TGTCTCTAAT 
GACGCCCAAG GAGTCGCTGA GGTCCAGCAG GATTTGACCT GCACCTTACC TCAGCAGTCT 
TTCTCTCATT CACCGTCATT CGCACCGGAT TGTGGAAACC CAGAGCCTGT AATCCCCGAC 
TCTGCCATCC ACTCCCCAGA ATCCTGGATG GAGAGTGAGC TGATGCTTGA GACTCACAGT 
GACAGTTCTC TGTTTGACAG CGGGCCGGGC TGGGATGTGT ACCGGGCCAC ACCTGTGCAG 
GTGACACCAG TGGATGAAGG ATTCATGTTG TCCATGGACG ACAGAGCTCC AGATGAACCC 
TTTGTGACGG GATCATACAG CGATGAAGGA GTCTCCTCGT TGGAGAGCAC CCAGGACAAA 
GCCCACCAGG AGGAACAGGA CAAGGAGCTG AACTCAAAGA ACCAGAATCT GGTTCAGGAA 
CTCATCATTG ATCCAAGTGA AGTTGACTTT TCAAAAGAGA CAGACCTCAG TCCCATCAGT 
ACAAGTCCAC TCCACAAAGA CAGTGAGCAG GAACGGTCCT GGACATTCAA TGAAGAATTA 
ACTCTCTCAG GTTCTGAAGA TTTCCTTAAA GATGGTCAAA CAAACCATTT TCAACCAGCC 
TTCTCCACAG ATGTTCCACC TCATGAAGAA CACACTGAAA GTTCTCAGAG AGAACAGGAA 
GGGAACAAAA AGAACCTAAA TGAACAAACT GATGGTCCAT CTGGGGAACT ACAGGAGAGG 
GGAACCTATG TGGAAGAGTA TCAAAAAGCT GACTCTTCAG TGGAAGATGA AGGAATCGAG 
GTAAAAGGTG GAAATGATCA AGACCAGAAA ACCCAGGAGG AAGAACTCTT AGCTAACGCC 
TCACATGATG AAGAAATCAG TAGTTCTCAG ATGATATTAA CTAATCAAGA GGGAGCATCA 
AAAACTGGTC TAATTATTCC CATCATTTCC ATCTCCAGTG AGCCAAAGGA GCACAAAGAA 
GAATCTGGCT CAGAAGACCA GGAAACAGAG GAGAAACTCC ATCAGCAGTT TGAGTCTGGA 
TATGGGGAAG ATGACACGGA ATTGAACAGT CCTACAAGTC CAGTTGCTCT GGAGCATGGT 
CGTCTGGAGG ATGGTCTTTT GGAGTGTGTT CCTCTGGAGC ACAGTCCTCT AGAGCATGTC 
TACAGTGAGG ACCTCAAAGA AGAATCTGAG GAGAGACCTT CCTGTCAGAT ATCAGAAAAT 
TCTGAAAGGA GGCCTTTGCA AACCACCCAC TCTGAAGACA AACTACTGTC TGATGGAGAT 
GAACCTCCCA GAAACTCTGG GTCAGAATGT GTGCAGAACC AGGAAATGGA TGAAGCCAGA 
CAAAAGCACA GCTCAGATGA TTTTGCAGCT GTAAAGAAGA CAATGGCTCA GGCAAATCCT 
GACAGTGATG CAAGTCAGGA ACCTGAGGGA CATGGAGTAA AACCAGCCGG TCCTGCCTCT 
GAAAGCGGCC CCCAAATCCC CCATCCGAGC ATACTCGACA CAGACACTTT AGACCTAAAC 
GAAGCAAAAG TTTCTCCATT TTCTGGACCA CCAGGATTAA ACAGAAACAT GGACTTGTTC 
TATTCCAAAT TTGACAAAAG CTCTCCCTCA GAGCATCTAG TTGGTGACCC AGTGGAACCC 
ATGGATCTGT TCTATCCTGA CAAAGAGGAG CCCATGCTCT CAGAACCTGC TGACACGGAG 
ATGCAGAGGT GGCCTTCAGT TCTGAGCGTC TCAGCTCTGG AGCCAGCGCC GCCGTCACTC 
GATGACATGG ATGACGAACA GCTGACGGGA GAGGAGGATG ATGATCAACA GCTGAATCCA 
TCCAGCCAGG GGCCTGCTGA TGCCTTCTCC ACCCAGGAGC AGTGCACGAT GTGCGGGGTG 
CCGATTGGGG AATCCCGGGT GGATTTTCCA GCAGACAGCA GGGAGTCTGA GAGTTGGAGA 
GATGCGGGAA GTTCCAGCAG AATAGACGAG ATCCAGACCC CTGTAGGTCT CCGGCGCAGG 
AAGGGTTCTC ACCTCGTGGA GTCCAGGGAC AACCAGAGAG CTGCATCAAG AACAGCCAAC 
AGAGATGGCA CTTGCTTTTG GTGGAACGAG AATTCGGACC TCTATCTGCT GCTGCTGCTC 
TGGGTGCTTC TGTACTGCCT CATGCTGCTG CCGCAGATGG ACCTAAAGGA GCTGCCGAGT 
CTTCTGCTGA ACCTCGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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clmn cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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