hectd1 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	hectd1
Entrez Gene ID	101168256
Full Name	HECT domain E3 ubiquitin protein ligase 1
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011490136.3	XP_011488438.1	E3 ubiquitin-protein ligase HECTD1 isoform X1
XM_011490137.2	XP_011488439.1	E3 ubiquitin-protein ligase HECTD1 isoform X2
XM_011490137.3	XP_011488439.1	E3 ubiquitin-protein ligase HECTD1 isoform X2
XM_020713930.2	XP_020569589.1	E3 ubiquitin-protein ligase HECTD1 isoform X3
XM_011490136.2	XP_011488438.1	E3 ubiquitin-protein ligase HECTD1 isoform X1
XM_020713930.1	XP_020569589.1	E3 ubiquitin-protein ligase HECTD1 isoform X3

Pathways from BioSystems
Gene Ontology

The following hectd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hectd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa31517	XM_011490136.3 Latest version!	Oryzias latipes HECT domain E3 ubiquitin protein ligase 1 (hectd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OJa31518	XM_011490137.3 Latest version!	Oryzias latipes HECT domain E3 ubiquitin protein ligase 1 (hectd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OJa76016	XM_020713930.2 Latest version!	Oryzias latipes HECT domain E3 ubiquitin protein ligase 1 (hectd1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
OJa31518	XM_011490137.2	Oryzias latipes HECT domain E3 ubiquitin protein ligase 1 (hectd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OJa31517	XM_011490136.2	Oryzias latipes HECT domain E3 ubiquitin protein ligase 1 (hectd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OJa76016	XM_020713930.1	Oryzias latipes HECT domain E3 ubiquitin protein ligase 1 (hectd1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hectd1 ( XM_011490136.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011488438.1 Oryzias latipes HECT domain E3 ubiquitin protein ligase 1 (hectd1), transcript variant X1, mRNA.

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CloneID	OJa31517
Clone ID Related Accession (Same CDS sequence)	XM_011490136.3 , XM_011490136.2
Accession Version	XM_011490136.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_011490136.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG TGGACCCAGA CACGTTGCTG GAGTGGCTGC AGATGGGACA GGGCGATGAG 
CGGGACATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGCTGCTCAT GTCGGACAAC 
GTGGACCGCT GCTTTGAGAC ATGTCCTCCT CGAACGTTCC TCCCAGCGCT TTGTAAGATC 
TTCTTGGACG AGAGCGCTCC TGATAACGTG CTGGAGGTCA CGGCCAGAGC CATCACCTAC 
TACCTGGACG TGTCGGCAGA ATGCACGCGG AGGATCGTGG GTGTGGACGG GGCCATAAAG 
GCCCTGTGCA ACCGGCTGGT GGTGGTGGAA CTCAACAACA GGACCAGCAG GGATCTGGCT 
GAGCAGTGTG TTAAGGTGCT GGAGCTGATC TGTACCAGGG AATCTGGCGC CGTGTTTGAA 
GCCAGCGGTC TGAACTGCGT TTTGAGCTTC ATACGCGACA GTGGTCACCT GGTCCACAAA 
GACACTCTGC ACTCTGCAAT GGCAGTGGTG TCTCGCCTCT GTAGCAAGAT GGAGCCGCAG 
GACTCTTCTC TAGAAACCTG CGTGGAGTCT CTGTCCAGCC TCCTGAAACA TGAGGATCAC 
CAGGTGTCAG ACGGTGCTCT GCGTTGCTTC GCCTCATTGG CTGATCGGTT TACTCGTCGA 
GGCGTAGACC CCGCCCCTCT GGCCAAACAT GGACTGACGG AGGAGCTGCT TTCCCGCATG 
GCGGCTGCCG GAGGTTCCGT GTCGGGCTCT TCTTCTGCCT GTAAACCAGG TCGGCCCTCC 
ACTGGTGCCA CACCGTCTGC CTCCGACTCC AAACTGAGCA ACCAGGTGTC CACCATCGTT 
AGCCTGCTGT CCACGCTCTG CCGGGGGTCG CCAGTGGTCA CTCATGACTT GTTACGTTCG 
GCACTCCCTA ACTCCATGGA GTCTGCGCTG GGAGGAGACG AGCGCTGTGT GCTGGACACC 
ATGCGGCTGG TCGACCTGCT GCTGGTGCTT CTGTTCGAGG GGCGGAAAGC GCTGCCAAAG 
TCCACCGCCG GCTCAGCAGG TCGGATCCCG GGGCTTCGCC GGCTGGACAG CTCAGGGGAG 
CGGTCGCACC GACAGCTCAT CGACTGCATC CGCAGCAAAG ACACGGACGC CCTGATAGAT 
GCCATCGACA CTGGAGCTTT TGAAGTGAAC TTCATGGACG ACGTGGGACA GACGCTGCTG 
AACTGGGCCT CAGCTTTTGG AACACAGGAA ATGGTGGAGT TTTTGTGCGA GAGGGGTGCA 
GATGTGAACC GAGGCCAGAG GTCATCCTCG CTGCACTACG CTGCCTGTTT CGGACGGCCA 
CAAGTTGCCA AGACGCTGCT GCGGCACGGA GCCAACCCGG ACCTGAGGGA TGAGGACGGG 
AAGACTCCTT TGGACAAAGC CAGGGAGAGG GGACACAGTG AGGTGGTGGC CATCCTTCAG 
TCTCCGGGAG ACTGGATGTG TCCAGTGAAC AAAGGAGACG ACAAGAGGAA GAAAGATGCG 
AGTAAGGAAG AAGAGGAGGG CAGTGAACCC AAAGGAGACC CAGAAATGGC TCCTTTCTAC 
CTGAAAAGAC TCCTCCCTGT TTTTGCACAA ACCTTTCAGC AAACCATGCT GCCTTCCATC 
AGGAAAGCCA GTCTGGCCCT CATCAGGAAG ATGGTTCACT ACAGCAGCGA GGTTCTGCTG 
AGGGAGGTGT GCGACAGTGA GACGGGACAC AACCTGCCCA CAGTGCTGGT GGAGATCACC 
GCCACCGTCC TCGACCAGGA GGATGATGAC GACGGTCACC TGCTGGCTCT GCAGATCATC 
AGGGATCTGG TGGACAAAGG TGGAGATGTT TTCCTTGACC AGCTGGCCCG ACTCGGGGTC 
ATCAACAAGG TGTCGACCTT GGCCGGACCG ACGTCTGATG ATGAGAACGA GGATGAAGTC 
AAACCTGAAA AGGAGGAGGA GGTGCAGGAA GATGCAAAGG AAATCCAGCA GGGGAAGCCG 
TACCACTGGA GGGACTGGTC CATCATCAGA GGCAGGGACT GCCTCTACAT TTGGTCAGAC 
GCCGCCGCCC TCGAGCTCTC CAACGGCTCC AATGGCTGGT TTCGCTTCAT CTTGGATGGG 
AAGCTCGCCA CCATGTACTC GAGTGGGAGC CCAGAGGGGG GGTCAGACAG CTCAGAGTCT 
CGCAGTGAGT TTTTGGAGAA GCTGCAGCGT GCCAGAAGTC AGGTGAAACC AGCAACAGCC 
AGCCAGCCCA TCCTCTCCAT GGTCGGACCC ACCAAGCTGA CGGTGGGGAA CTGGTCTCTG 
ACCTGCCTGA AGGAAGGCGA GATTGCAATT CACAACTCAG ACGGGCAGCA GGCCACGATC 
CTGAAGGAGG ATCTGCCCGG CTTCGTGTTC GAGTCCAACA GAGGAACCAA ACACTCGTTC 
ACTGCTGAAA CGTCGCTCGG TTCTGAGTTT GTCACTGGCT GGACGGGGAA ACGTGGCAGA 
AAGCTGAAGT CCAAGCTGGA GAAGACCAAG CAGAAGGTGA AGAGCATGGC CAGGGAGCTG 
TACGACGACC ACTTCAAAGC TGTGGAGAGC ATGCCCAGAG GAGTGGTGGT CACCCTGAGG 
AACATCGCCA CGCAGCTGGA GTCCGCCTGG GAGCTGCACA TAAACCGACA GTGTCTGGAA 
GGAGAGAACA CGTGGAGGGA CCTGATGAAG ACCGCTCTGG AGAACCTGAT TGTGGTTTTG 
AAGGATGAAA ACACGATTTC TCCGTATGAA ATGTGCAGCA GCGGCCTGGT TCAGGCTCTG 
TTTACCGTCC TCAGCAACAG TGTGGAGCTG GACCTGAAAC ATGACTGTAA GCCTCTAATG 
GAAAGGATCA ACGTCTTTAA AGCTGCTTTC AGCGAGAATG AAGACAATGA AAGCCGACCA 
GCCGTTGCCT TAGTGCGGAA GCTGATAGCC GTCCTGGAAT CGATCGAGCG TCTGCCCCTG 
CACCTGTATG ACACGCCTGG ATCCTCCTAC AATCTGCAGA TTCTGACGAG GAGGCTGCGG 
TTCCGTCTGG AGCGAGCGCC TGGGGAGACC GCTCTCATTG ACCGGACGGG ACGCATGCTG 
AAGATGGAGC CGCTCGCCAC CGTAGAGTCT CTGGAGCAGT ACCTGCTGAA AATGGTCGCT 
AAACAGTGGT ACGACTTTGA ACGCTCATCC TTCGTTTTTG TGAGAAAACT GAGAGAAGGA 
CAGATCTTCA CCTTCAGGCA CCAGCACGAC TTTGACGAGA ACGGCATCAT CTACTGGGTC 
GGAACCAACG CCAAGACGGC GTACGAGTGG GTGAACCCCG CAGCGTACGG CCTGGTGGTG 
GTGACCTCCT CCGAGGGGAG GAATCTGCCT TATGGGCGTT TGGAGGACAT CCTGAGTCGG 
GATAGCTCCG CCCTCAACTG CCACACGAAC GACGACAAGA ACGCCTGGTT CGCAGTTGAC 
CTCGGCCTCT GGGTGATTCC GTCAGCGTAC ACTCTGAGGC ACGCCAGGGG GTACGGACGC 
TCTGCGCTCA GGAACTGGGT TTTCCAAGTG TCCAAGGACG GACAGAACTG GTCCACACTC 
TACAACCACA TCGACGATGG CAGTCTCAAT GAACCGGGGT CTACGGCCAC GTGGCCTCTG 
GACCCGTCCA AAGAGGAGAA GCAGGGCTGG AGACACATCC GCATCAAGCA GATGGGGAAG 
AACGCCAGCG GTCAGACACA CTACCTGTCC TTATCTGGGC TGGAGCTGTA CGGCACGGTC 
ACCGCCGTAT GCGAGGACCA GCTGGGTAAA GCTGTGAAAG AGGCGGAGGC CAACCTTCGT 
CGCCAGCGGC GCCTCTTCCG TTCTCAGGTC ATGAAGTACA TCGTCCCAGG TGCGCGGGTC 
ATCCGTGGCA TCGACTGGAA GTGGAGGGAC CAGGATGGAA ACCCGGCAGG GGAAGGCACT 
GTCACTGGGG AGGCGCACAA CGGCTGGATT GATGTAACCT GGGATGCTGG CGGCTCTAAC 
TCTTATCGTA TGGGCGCCGA AGGGAAGTTT GACCTCAAGC TTGCTCCAGG GTACGACCCT 
GAGCTGGCTG CCACAGCGCC ATCACCCAAA CCTGTCTCAT CCGCTGTTTC AGGTCCCGCC 
TCCTCCACGG TGGGACCCTC TGCGACGCCA GCAGCTGTTG GCGGAACCAC CATCACCACC 
TCATCCTCCT CCACCACCTC ATCGTCCTCG CAGCAGCCTT CCTGGAGCAG TTTGGTGAAA 
AATAACTGTC CCGACAAGGG AGGGGCCAGC TCCTCCAGCA GGAAGGGTAG CAGCAGCTCC 
GTCTGCAGCG TCGCTTCCTC CTCTGACATC AGTCTGAGCT CCTCCATTGG GCTGCCTGGC 
GCTGGGAGCC TGCAGCTGGA CAGGAAGGCC GAGGGGCTGC TGCTAGATCA GGCCGCGGGG 
GTGGAAGGGG TGACGGGGGG AGGGACCGGT TCCGATGGAC ATCAGCAAGA GCCGATAGTC 
GTTCTGTCCT CCGTGACCGA ATCCGGTTCG GCGTCCAGCT CCGGCACGCT CACCGCCGAC 
GTGTCAACGG CGGGTGACGA GCATCACTCC CCCTCTGCAA TGGCCACTGC TGTGGACCCG 
GCCACGGCCA TCTCCATGGG CTTGGTGAGC GTCAGCTCCC CGGACGTCAG CTCCGTGTCG 
GAGCCCTCCA GCAAAGACAC TCACTCCCAG AGGCCGCTGT GCTCGGCCGT GAACACCCGG 
CTGTCCGTCA GCTCCCTGCT GGCCGCCAGC GCTCCCATGA GCTCCAGCGC CAGCGTGCCC 
AATCTGTCCT CTCGGGAGGC CAGCCTCATG GAGTCCTTCG TCCGCCGCGC GCCCAACATG 
TCCCGCACCA ACGCTACCAA CAACATGAAC CTGAGCCGCA GCAGCAGCGA CAACAACACC 
AACACACTGG GCAGGAACGT GATGAGCACA GCCACATCTC CTCTTATGGG CGCTCAGAGC 
TTTCCTAACC TCACCACCAC TGGCACCACC TCCACCGTTA CCATGTCAAC CTCCATAGTA 
ACCTGCGGTA ATAATGTAGC CACAGCCACC ACGGGGCTGT CTGTGGGACA GTTGCTAAGC 
AACACACTGA CAACCAGCTT AACCTCCACA TCCAGCGAGA GCGACACGGG ACAGGAGGCC 
GAGTTCTCCC TCTATGACTT CCTGGACAGT TGCCGGGCCA ACACGCTGCT AGCAGAGCTG 
GACGATGAGG AGGACCTCCC AGAGCCCGAC GACGACGACG ATGAGAATGA AGACGACAAT 
CAGGAGGATC AGGAGTATGA GGAGGTCCTG GAGGAGGAGG AGTATGAGAC GAAGGGAGGA 
CGGAGGAGGA CATGGGACGA TGACTTTGTC CTAAAAAGAC AGTTTTCTGC TTTGGTCCCT 
GCCTTCGACC CCAGACCAGG AAGAACCAAC GTCCAGCAGA CCACCGACCT GGAGATCCCC 
CCACCAGGAA CTCCGCGGTC GGAGGTTCGG GAGGAGGTGG AGTGTGCCCC ATCCCCCCAC 
CTCGCCCTCA CACTGAAGGT GGCGGGTCTC GGCACGGCTC GGGAGGTGGA GATCCCGCTC 
TCCAACTACA AGTTGACCAT CTTCTACTAT GTGCAGCGGC TCCTGCAGCT CTCCTGCAAC 
GGCGCCGTAA AGACGGACAA GCTGCGGCGC ATCTGGGAGC CGACGTACAC GATCATGTAC 
AGAGAGCTCA AAGACTCGGA CAAAGAGAAG GAGAGCGGGA AGATGGACCT CTTTGAGCAC 
GTTGTTGGAA GTCGTTCTGG AGGTCTGAGC CCCAGCTCGG GTTCAGCCAA TCAGAGCAGC 
GAGATCCTGG GCTGGGCGAA GGAGGCGGTC CTGGCCAAGG CGGGCTGCAG CCAGAACGCC 
TGCGGGGTGG AAGACGTCCT GCAGCTGCTG CGCATTCTCT ATGTGATTGG GAGAGACTCT 
ACTGCAGGCG GACGCACGCT GCAAGAGGAT GTTGACGAGC TGCAGTTCAA CTCGCCTCCT 
GAGGAGTTCA GCAGCAAGAA GATCACCACC AAGATCCTGC AGCAGATCGA GGAGCCCTTG 
GCTCTGGCTA GCGGAGCGCT GCCGGACTGG TGTGAGCAGC TCACCTCCAA GTGTCCTTTC 
CTGATCCCCT TTGAGACCAG ACAGCTGTAC TTCACCTGCA CTGCCTTTGG GGCCTCCAGG 
GCGATCGTGT GGCTCCAGAA CCGCCGCGAG GCGACCATGG AGCGCTCACG GCCATCCACC 
ACCGTCCGGC GAGACGACCC CGGAGAGTTC AGAGTCGGTC GGCTTAAACA CGAGCGCGTT 
AAAGTTCCTC GAGGGGAGTC CATGATGGAG TGGGCGGAGT CTGTGATGCA GATCCACGCA 
AACAGGAAGT CTGTTTTAGA GGTGGAGTTT CTGGGTGAGG AGGGGACGGG TCTGGGTCCG 
ACTCTGGAGT TCTACGCTCT GGTGGCGGCG GAGTTTCAGA GAACGTCTCT GGGGATCTGG 
CTCTGTGACG ACGACTTCCC GGACGACGAG TCCCGACAGG TGGATCTGGG TGGAGGTCTG 
AAGCCTCCTG GCTTCTACGT GCAGCGGTCC TGCGGGCTCT TCCCGGCGCC GTTCCCTCAG 
GACAGCGAGG AGCTGGAGCG GATTACGCGG CTCTTCCACT TCCTGGGGAT CTTCCTGGCC 
AAGTGCATCC AGGACAACCG GCTGGTGGAC CTGCCCGTGT CCCAGCCTTT CTTCAAGCTG 
CTCTGCATGG GGGACATCAA GTCCAACATG AGCAAGCTGC TGTACCAGTC CCGCGGCTCC 
CCCCAGGGCC ACCTCTCCGA GAGGGCCCCC CTGCTGCTGC TGACGGAGGT GCAGTCGGAG 
GCGTCCACAG AGGAGAGCCA GGAGACCTAC TCTGTGGGGA GCTTCGACGA GGACTCCAAG 
TCAGACTTCA TCATGGACCC CCCCAAACCC AAGCCGCCCG CCTGGTTCCA CGGCATCATG 
TCCTGGGACG ACTTCCATCT GGTCAACCCG CACAGAGCCA GTTTCCTGAA GGAGGTGAAG 
GAGCTGGCAG TGAGGAGGAG ACAGATTCTG ACCAGCAAGA GTTTGTCAGA GGATGAGAAG 
AACACCAGGC TGCAGGACCT GATGCTGAGG AACCCGCTGG GCTCTGGGCC CCCCTTAAGC 
GTCGAAGACC TCGGGTTGAA CTTCCAGTTC TGTCCGTCCT CCAAGATTCA CGGCTTCTTC 
GCGGTGGATC TGAAACCAAA CGGGGATGAC GAGATGGTGA GTCTGGAGAA CGCCGAGGAG 
TACGTGGAGC TGATGTTCGA CTTCTGCATG CACGCCGGCA TCCAGAAGCA GATGGAGGCG 
TTCAGAGAGG GTTTCAACCG CGTCTTCCCC ATGGAGAAGC TCAGCTCGTT CAGCCAGAAG 
GAGGTGCAGA TGATCCTCTG CGGAAACCAA TCCCCCTGCT GGACCGCCGA TGACATCATC 
AGCTACACCG AGCCAAAGCT GGGATACACC AGAGACAGCC CTGGGTTCCT GCGCTTCATC 
CGAGTTTTAT GCGGAATGTC GACGGACGAG AGGAAAGCTT TCCTGCAGTT CACCACCGGC 
TGCTCCACGC TGCCCCCCGG TGGCCTGGCT AACCTGCACC CGCGCCTCAC CATCGTCAGA 
AAGGTGGACG CCACGGACTC CAGCTACCCA TCGGTGAACA CGTGCGTTCA CTACCTGAAG 
CTGCCGGAGT ACTCGTCAGA GGAGATCATG AGGGAGCGGC TGTTGGCGGC CACCATGGAG 
AAGGGCTTCC ACCTCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011488438.1
CDS	458..8158
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEASGLNCVLSFIRDSGHLVHKDTLHSAMAVVSRLCSKMEPQDSSLETCVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGSVSGSSSACKPGRPSTGATPSASDSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSALPNSMESALGGDERCVLDTMRLVDLLLVLLFEGRKALPKSTAGSAGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKRKKDASKEEEEGSEPKGDPEMAPFYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMVHYSSEVLLREVCDSETGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDVFLDQLARLGVINKVSTLAGPTSDDENEDEVKPEKEEEVQEDAKEIQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARSQVKPATASQPILSMVGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVKSMARELYDDHFKAVESMPRGVVVTLRNIATQLESAWELHINRQCLEGENTWRDLMKTALENLIVVLKDENTISPYEMCSSGLVQALFTVLSNSVELDLKHDCKPLMERINVFKAAFSENEDNESRPAVALVRKLIAVLESIERLPLHLYDTPGSSYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFERSSFVFVRKLREGQIFTFRHQHDFDENGIIYWVGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDSSALNCHTNDDKNAWFAVDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWSTLYNHIDDGSLNEPGSTATWPLDPSKEEKQGWRHIRIKQMGKNASGQTHYLSLSGLELYGTVTAVCEDQLGKAVKEAEANLRRQRRLFRSQVMKYIVPGARVIRGIDWKWRDQDGNPAGEGTVTGEAHNGWIDVTWDAGGSNSYRMGAEGKFDLKLAPGYDPELAATAPSPKPVSSAVSGPASSTVGPSATPAAVGGTTITTSSSSTTSSSSQQPSWSSLVKNNCPDKGGASSSSRKGSSSSVCSVASSSDISLSSSIGLPGAGSLQLDRKAEGLLLDQAAGVEGVTGGGTGSDGHQQEPIVVLSSVTESGSASSSGTLTADVSTAGDEHHSPSAMATAVDPATAISMGLVSVSSPDVSSVSEPSSKDTHSQRPLCSAVNTRLSVSSLLAASAPMSSSASVPNLSSREASLMESFVRRAPNMSRTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTTGTTSTVTMSTSIVTCGNNVATATTGLSVGQLLSNTLTTSLTSTSSESDTGQEAEFSLYDFLDSCRANTLLAELDDEEDLPEPDDDDDENEDDNQEDQEYEEVLEEEEYETKGGRRRTWDDDFVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPRSEVREEVECAPSPHLALTLKVAGLGTAREVEIPLSNYKLTIFYYVQRLLQLSCNGAVKTDKLRRIWEPTYTIMYRELKDSDKEKESGKMDLFEHVVGSRSGGLSPSSGSANQSSEILGWAKEAVLAKAGCSQNACGVEDVLQLLRILYVIGRDSTAGGRTLQEDVDELQFNSPPEEFSSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATMERSRPSTTVRRDDPGEFRVGRLKHERVKVPRGESMMEWAESVMQIHANRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTSLGIWLCDDDFPDDESRQVDLGGGLKPPGFYVQRSCGLFPAPFPQDSEELERITRLFHFLGIFLAKCIQDNRLVDLPVSQPFFKLLCMGDIKSNMSKLLYQSRGSPQGHLSERAPLLLLTEVQSEASTEESQETYSVGSFDEDSKSDFIMDPPKPKPPAWFHGIMSWDDFHLVNPHRASFLKEVKELAVRRRQILTSKSLSEDEKNTRLQDLMLRNPLGSGPPLSVEDLGLNFQFCPSSKIHGFFAVDLKPNGDDEMVSLENAEEYVELMFDFCMHAGIQKQMEAFREGFNRVFPMEKLSSFSQKEVQMILCGNQSPCWTADDIISYTEPKLGYTRDSPGFLRFIRVLCGMSTDERKAFLQFTTGCSTLPPGGLANLHPRLTIVRKVDATDSSYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_011490136.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes HECT domain E3 ubiquitin protein ligase 1 (hectd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011490136.3

ORF Insert Sequence:

ATGGCGGATG TGGACCCAGA CACGTTGCTG GAGTGGCTGC AGATGGGACA GGGCGATGAG 
CGGGACATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGCTGCTCAT GTCGGACAAC 
GTGGACCGCT GCTTTGAGAC ATGTCCTCCT CGAACGTTCC TCCCAGCGCT TTGTAAGATC 
TTCTTGGACG AGAGCGCTCC TGATAACGTG CTGGAGGTCA CGGCCAGAGC CATCACCTAC 
TACCTGGACG TGTCGGCAGA ATGCACGCGG AGGATCGTGG GTGTGGACGG GGCCATAAAG 
GCCCTGTGCA ACCGGCTGGT GGTGGTGGAA CTCAACAACA GGACCAGCAG GGATCTGGCT 
GAGCAGTGTG TTAAGGTGCT GGAGCTGATC TGTACCAGGG AATCTGGCGC CGTGTTTGAA 
GCCAGCGGTC TGAACTGCGT TTTGAGCTTC ATACGCGACA GTGGTCACCT GGTCCACAAA 
GACACTCTGC ACTCTGCAAT GGCAGTGGTG TCTCGCCTCT GTAGCAAGAT GGAGCCGCAG 
GACTCTTCTC TAGAAACCTG CGTGGAGTCT CTGTCCAGCC TCCTGAAACA TGAGGATCAC 
CAGGTGTCAG ACGGTGCTCT GCGTTGCTTC GCCTCATTGG CTGATCGGTT TACTCGTCGA 
GGCGTAGACC CCGCCCCTCT GGCCAAACAT GGACTGACGG AGGAGCTGCT TTCCCGCATG 
GCGGCTGCCG GAGGTTCCGT GTCGGGCTCT TCTTCTGCCT GTAAACCAGG TCGGCCCTCC 
ACTGGTGCCA CACCGTCTGC CTCCGACTCC AAACTGAGCA ACCAGGTGTC CACCATCGTT 
AGCCTGCTGT CCACGCTCTG CCGGGGGTCG CCAGTGGTCA CTCATGACTT GTTACGTTCG 
GCACTCCCTA ACTCCATGGA GTCTGCGCTG GGAGGAGACG AGCGCTGTGT GCTGGACACC 
ATGCGGCTGG TCGACCTGCT GCTGGTGCTT CTGTTCGAGG GGCGGAAAGC GCTGCCAAAG 
TCCACCGCCG GCTCAGCAGG TCGGATCCCG GGGCTTCGCC GGCTGGACAG CTCAGGGGAG 
CGGTCGCACC GACAGCTCAT CGACTGCATC CGCAGCAAAG ACACGGACGC CCTGATAGAT 
GCCATCGACA CTGGAGCTTT TGAAGTGAAC TTCATGGACG ACGTGGGACA GACGCTGCTG 
AACTGGGCCT CAGCTTTTGG AACACAGGAA ATGGTGGAGT TTTTGTGCGA GAGGGGTGCA 
GATGTGAACC GAGGCCAGAG GTCATCCTCG CTGCACTACG CTGCCTGTTT CGGACGGCCA 
CAAGTTGCCA AGACGCTGCT GCGGCACGGA GCCAACCCGG ACCTGAGGGA TGAGGACGGG 
AAGACTCCTT TGGACAAAGC CAGGGAGAGG GGACACAGTG AGGTGGTGGC CATCCTTCAG 
TCTCCGGGAG ACTGGATGTG TCCAGTGAAC AAAGGAGACG ACAAGAGGAA GAAAGATGCG 
AGTAAGGAAG AAGAGGAGGG CAGTGAACCC AAAGGAGACC CAGAAATGGC TCCTTTCTAC 
CTGAAAAGAC TCCTCCCTGT TTTTGCACAA ACCTTTCAGC AAACCATGCT GCCTTCCATC 
AGGAAAGCCA GTCTGGCCCT CATCAGGAAG ATGGTTCACT ACAGCAGCGA GGTTCTGCTG 
AGGGAGGTGT GCGACAGTGA GACGGGACAC AACCTGCCCA CAGTGCTGGT GGAGATCACC 
GCCACCGTCC TCGACCAGGA GGATGATGAC GACGGTCACC TGCTGGCTCT GCAGATCATC 
AGGGATCTGG TGGACAAAGG TGGAGATGTT TTCCTTGACC AGCTGGCCCG ACTCGGGGTC 
ATCAACAAGG TGTCGACCTT GGCCGGACCG ACGTCTGATG ATGAGAACGA GGATGAAGTC 
AAACCTGAAA AGGAGGAGGA GGTGCAGGAA GATGCAAAGG AAATCCAGCA GGGGAAGCCG 
TACCACTGGA GGGACTGGTC CATCATCAGA GGCAGGGACT GCCTCTACAT TTGGTCAGAC 
GCCGCCGCCC TCGAGCTCTC CAACGGCTCC AATGGCTGGT TTCGCTTCAT CTTGGATGGG 
AAGCTCGCCA CCATGTACTC GAGTGGGAGC CCAGAGGGGG GGTCAGACAG CTCAGAGTCT 
CGCAGTGAGT TTTTGGAGAA GCTGCAGCGT GCCAGAAGTC AGGTGAAACC AGCAACAGCC 
AGCCAGCCCA TCCTCTCCAT GGTCGGACCC ACCAAGCTGA CGGTGGGGAA CTGGTCTCTG 
ACCTGCCTGA AGGAAGGCGA GATTGCAATT CACAACTCAG ACGGGCAGCA GGCCACGATC 
CTGAAGGAGG ATCTGCCCGG CTTCGTGTTC GAGTCCAACA GAGGAACCAA ACACTCGTTC 
ACTGCTGAAA CGTCGCTCGG TTCTGAGTTT GTCACTGGCT GGACGGGGAA ACGTGGCAGA 
AAGCTGAAGT CCAAGCTGGA GAAGACCAAG CAGAAGGTGA AGAGCATGGC CAGGGAGCTG 
TACGACGACC ACTTCAAAGC TGTGGAGAGC ATGCCCAGAG GAGTGGTGGT CACCCTGAGG 
AACATCGCCA CGCAGCTGGA GTCCGCCTGG GAGCTGCACA TAAACCGACA GTGTCTGGAA 
GGAGAGAACA CGTGGAGGGA CCTGATGAAG ACCGCTCTGG AGAACCTGAT TGTGGTTTTG 
AAGGATGAAA ACACGATTTC TCCGTATGAA ATGTGCAGCA GCGGCCTGGT TCAGGCTCTG 
TTTACCGTCC TCAGCAACAG TGTGGAGCTG GACCTGAAAC ATGACTGTAA GCCTCTAATG 
GAAAGGATCA ACGTCTTTAA AGCTGCTTTC AGCGAGAATG AAGACAATGA AAGCCGACCA 
GCCGTTGCCT TAGTGCGGAA GCTGATAGCC GTCCTGGAAT CGATCGAGCG TCTGCCCCTG 
CACCTGTATG ACACGCCTGG ATCCTCCTAC AATCTGCAGA TTCTGACGAG GAGGCTGCGG 
TTCCGTCTGG AGCGAGCGCC TGGGGAGACC GCTCTCATTG ACCGGACGGG ACGCATGCTG 
AAGATGGAGC CGCTCGCCAC CGTAGAGTCT CTGGAGCAGT ACCTGCTGAA AATGGTCGCT 
AAACAGTGGT ACGACTTTGA ACGCTCATCC TTCGTTTTTG TGAGAAAACT GAGAGAAGGA 
CAGATCTTCA CCTTCAGGCA CCAGCACGAC TTTGACGAGA ACGGCATCAT CTACTGGGTC 
GGAACCAACG CCAAGACGGC GTACGAGTGG GTGAACCCCG CAGCGTACGG CCTGGTGGTG 
GTGACCTCCT CCGAGGGGAG GAATCTGCCT TATGGGCGTT TGGAGGACAT CCTGAGTCGG 
GATAGCTCCG CCCTCAACTG CCACACGAAC GACGACAAGA ACGCCTGGTT CGCAGTTGAC 
CTCGGCCTCT GGGTGATTCC GTCAGCGTAC ACTCTGAGGC ACGCCAGGGG GTACGGACGC 
TCTGCGCTCA GGAACTGGGT TTTCCAAGTG TCCAAGGACG GACAGAACTG GTCCACACTC 
TACAACCACA TCGACGATGG CAGTCTCAAT GAACCGGGGT CTACGGCCAC GTGGCCTCTG 
GACCCGTCCA AAGAGGAGAA GCAGGGCTGG AGACACATCC GCATCAAGCA GATGGGGAAG 
AACGCCAGCG GTCAGACACA CTACCTGTCC TTATCTGGGC TGGAGCTGTA CGGCACGGTC 
ACCGCCGTAT GCGAGGACCA GCTGGGTAAA GCTGTGAAAG AGGCGGAGGC CAACCTTCGT 
CGCCAGCGGC GCCTCTTCCG TTCTCAGGTC ATGAAGTACA TCGTCCCAGG TGCGCGGGTC 
ATCCGTGGCA TCGACTGGAA GTGGAGGGAC CAGGATGGAA ACCCGGCAGG GGAAGGCACT 
GTCACTGGGG AGGCGCACAA CGGCTGGATT GATGTAACCT GGGATGCTGG CGGCTCTAAC 
TCTTATCGTA TGGGCGCCGA AGGGAAGTTT GACCTCAAGC TTGCTCCAGG GTACGACCCT 
GAGCTGGCTG CCACAGCGCC ATCACCCAAA CCTGTCTCAT CCGCTGTTTC AGGTCCCGCC 
TCCTCCACGG TGGGACCCTC TGCGACGCCA GCAGCTGTTG GCGGAACCAC CATCACCACC 
TCATCCTCCT CCACCACCTC ATCGTCCTCG CAGCAGCCTT CCTGGAGCAG TTTGGTGAAA 
AATAACTGTC CCGACAAGGG AGGGGCCAGC TCCTCCAGCA GGAAGGGTAG CAGCAGCTCC 
GTCTGCAGCG TCGCTTCCTC CTCTGACATC AGTCTGAGCT CCTCCATTGG GCTGCCTGGC 
GCTGGGAGCC TGCAGCTGGA CAGGAAGGCC GAGGGGCTGC TGCTAGATCA GGCCGCGGGG 
GTGGAAGGGG TGACGGGGGG AGGGACCGGT TCCGATGGAC ATCAGCAAGA GCCGATAGTC 
GTTCTGTCCT CCGTGACCGA ATCCGGTTCG GCGTCCAGCT CCGGCACGCT CACCGCCGAC 
GTGTCAACGG CGGGTGACGA GCATCACTCC CCCTCTGCAA TGGCCACTGC TGTGGACCCG 
GCCACGGCCA TCTCCATGGG CTTGGTGAGC GTCAGCTCCC CGGACGTCAG CTCCGTGTCG 
GAGCCCTCCA GCAAAGACAC TCACTCCCAG AGGCCGCTGT GCTCGGCCGT GAACACCCGG 
CTGTCCGTCA GCTCCCTGCT GGCCGCCAGC GCTCCCATGA GCTCCAGCGC CAGCGTGCCC 
AATCTGTCCT CTCGGGAGGC CAGCCTCATG GAGTCCTTCG TCCGCCGCGC GCCCAACATG 
TCCCGCACCA ACGCTACCAA CAACATGAAC CTGAGCCGCA GCAGCAGCGA CAACAACACC 
AACACACTGG GCAGGAACGT GATGAGCACA GCCACATCTC CTCTTATGGG CGCTCAGAGC 
TTTCCTAACC TCACCACCAC TGGCACCACC TCCACCGTTA CCATGTCAAC CTCCATAGTA 
ACCTGCGGTA ATAATGTAGC CACAGCCACC ACGGGGCTGT CTGTGGGACA GTTGCTAAGC 
AACACACTGA CAACCAGCTT AACCTCCACA TCCAGCGAGA GCGACACGGG ACAGGAGGCC 
GAGTTCTCCC TCTATGACTT CCTGGACAGT TGCCGGGCCA ACACGCTGCT AGCAGAGCTG 
GACGATGAGG AGGACCTCCC AGAGCCCGAC GACGACGACG ATGAGAATGA AGACGACAAT 
CAGGAGGATC AGGAGTATGA GGAGGTCCTG GAGGAGGAGG AGTATGAGAC GAAGGGAGGA 
CGGAGGAGGA CATGGGACGA TGACTTTGTC CTAAAAAGAC AGTTTTCTGC TTTGGTCCCT 
GCCTTCGACC CCAGACCAGG AAGAACCAAC GTCCAGCAGA CCACCGACCT GGAGATCCCC 
CCACCAGGAA CTCCGCGGTC GGAGGTTCGG GAGGAGGTGG AGTGTGCCCC ATCCCCCCAC 
CTCGCCCTCA CACTGAAGGT GGCGGGTCTC GGCACGGCTC GGGAGGTGGA GATCCCGCTC 
TCCAACTACA AGTTGACCAT CTTCTACTAT GTGCAGCGGC TCCTGCAGCT CTCCTGCAAC 
GGCGCCGTAA AGACGGACAA GCTGCGGCGC ATCTGGGAGC CGACGTACAC GATCATGTAC 
AGAGAGCTCA AAGACTCGGA CAAAGAGAAG GAGAGCGGGA AGATGGACCT CTTTGAGCAC 
GTTGTTGGAA GTCGTTCTGG AGGTCTGAGC CCCAGCTCGG GTTCAGCCAA TCAGAGCAGC 
GAGATCCTGG GCTGGGCGAA GGAGGCGGTC CTGGCCAAGG CGGGCTGCAG CCAGAACGCC 
TGCGGGGTGG AAGACGTCCT GCAGCTGCTG CGCATTCTCT ATGTGATTGG GAGAGACTCT 
ACTGCAGGCG GACGCACGCT GCAAGAGGAT GTTGACGAGC TGCAGTTCAA CTCGCCTCCT 
GAGGAGTTCA GCAGCAAGAA GATCACCACC AAGATCCTGC AGCAGATCGA GGAGCCCTTG 
GCTCTGGCTA GCGGAGCGCT GCCGGACTGG TGTGAGCAGC TCACCTCCAA GTGTCCTTTC 
CTGATCCCCT TTGAGACCAG ACAGCTGTAC TTCACCTGCA CTGCCTTTGG GGCCTCCAGG 
GCGATCGTGT GGCTCCAGAA CCGCCGCGAG GCGACCATGG AGCGCTCACG GCCATCCACC 
ACCGTCCGGC GAGACGACCC CGGAGAGTTC AGAGTCGGTC GGCTTAAACA CGAGCGCGTT 
AAAGTTCCTC GAGGGGAGTC CATGATGGAG TGGGCGGAGT CTGTGATGCA GATCCACGCA 
AACAGGAAGT CTGTTTTAGA GGTGGAGTTT CTGGGTGAGG AGGGGACGGG TCTGGGTCCG 
ACTCTGGAGT TCTACGCTCT GGTGGCGGCG GAGTTTCAGA GAACGTCTCT GGGGATCTGG 
CTCTGTGACG ACGACTTCCC GGACGACGAG TCCCGACAGG TGGATCTGGG TGGAGGTCTG 
AAGCCTCCTG GCTTCTACGT GCAGCGGTCC TGCGGGCTCT TCCCGGCGCC GTTCCCTCAG 
GACAGCGAGG AGCTGGAGCG GATTACGCGG CTCTTCCACT TCCTGGGGAT CTTCCTGGCC 
AAGTGCATCC AGGACAACCG GCTGGTGGAC CTGCCCGTGT CCCAGCCTTT CTTCAAGCTG 
CTCTGCATGG GGGACATCAA GTCCAACATG AGCAAGCTGC TGTACCAGTC CCGCGGCTCC 
CCCCAGGGCC ACCTCTCCGA GAGGGCCCCC CTGCTGCTGC TGACGGAGGT GCAGTCGGAG 
GCGTCCACAG AGGAGAGCCA GGAGACCTAC TCTGTGGGGA GCTTCGACGA GGACTCCAAG 
TCAGACTTCA TCATGGACCC CCCCAAACCC AAGCCGCCCG CCTGGTTCCA CGGCATCATG 
TCCTGGGACG ACTTCCATCT GGTCAACCCG CACAGAGCCA GTTTCCTGAA GGAGGTGAAG 
GAGCTGGCAG TGAGGAGGAG ACAGATTCTG ACCAGCAAGA GTTTGTCAGA GGATGAGAAG 
AACACCAGGC TGCAGGACCT GATGCTGAGG AACCCGCTGG GCTCTGGGCC CCCCTTAAGC 
GTCGAAGACC TCGGGTTGAA CTTCCAGTTC TGTCCGTCCT CCAAGATTCA CGGCTTCTTC 
GCGGTGGATC TGAAACCAAA CGGGGATGAC GAGATGGTGA GTCTGGAGAA CGCCGAGGAG 
TACGTGGAGC TGATGTTCGA CTTCTGCATG CACGCCGGCA TCCAGAAGCA GATGGAGGCG 
TTCAGAGAGG GTTTCAACCG CGTCTTCCCC ATGGAGAAGC TCAGCTCGTT CAGCCAGAAG 
GAGGTGCAGA TGATCCTCTG CGGAAACCAA TCCCCCTGCT GGACCGCCGA TGACATCATC 
AGCTACACCG AGCCAAAGCT GGGATACACC AGAGACAGCC CTGGGTTCCT GCGCTTCATC 
CGAGTTTTAT GCGGAATGTC GACGGACGAG AGGAAAGCTT TCCTGCAGTT CACCACCGGC 
TGCTCCACGC TGCCCCCCGG TGGCCTGGCT AACCTGCACC CGCGCCTCAC CATCGTCAGA 
AAGGTGGACG CCACGGACTC CAGCTACCCA TCGGTGAACA CGTGCGTTCA CTACCTGAAG 
CTGCCGGAGT ACTCGTCAGA GGAGATCATG AGGGAGCGGC TGTTGGCGGC CACCATGGAG 
AAGGGCTTCC ACCTCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hectd1 ( XM_011490136.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011488438.1 Oryzias latipes HECT domain E3 ubiquitin protein ligase 1 (hectd1), transcript variant X1, mRNA.

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CloneID	OJa31517
Clone ID Related Accession (Same CDS sequence)	XM_011490136.3 , XM_011490136.2
Accession Version	XM_011490136.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011490136.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG TGGACCCAGA CACGTTGCTG GAGTGGCTGC AGATGGGACA GGGCGATGAG 
CGGGACATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGCTGCTCAT GTCGGACAAC 
GTGGACCGCT GCTTTGAGAC ATGTCCTCCT CGAACGTTCC TCCCAGCGCT TTGTAAGATC 
TTCTTGGACG AGAGCGCTCC TGATAACGTG CTGGAGGTCA CGGCCAGAGC CATCACCTAC 
TACCTGGACG TGTCGGCAGA ATGCACGCGG AGGATCGTGG GTGTGGACGG GGCCATAAAG 
GCCCTGTGCA ACCGGCTGGT GGTGGTGGAA CTCAACAACA GGACCAGCAG GGATCTGGCT 
GAGCAGTGTG TTAAGGTGCT GGAGCTGATC TGTACCAGGG AATCTGGCGC CGTGTTTGAA 
GCCAGCGGTC TGAACTGCGT TTTGAGCTTC ATACGCGACA GTGGTCACCT GGTCCACAAA 
GACACTCTGC ACTCTGCAAT GGCAGTGGTG TCTCGCCTCT GTAGCAAGAT GGAGCCGCAG 
GACTCTTCTC TAGAAACCTG CGTGGAGTCT CTGTCCAGCC TCCTGAAACA TGAGGATCAC 
CAGGTGTCAG ACGGTGCTCT GCGTTGCTTC GCCTCATTGG CTGATCGGTT TACTCGTCGA 
GGCGTAGACC CCGCCCCTCT GGCCAAACAT GGACTGACGG AGGAGCTGCT TTCCCGCATG 
GCGGCTGCCG GAGGTTCCGT GTCGGGCTCT TCTTCTGCCT GTAAACCAGG TCGGCCCTCC 
ACTGGTGCCA CACCGTCTGC CTCCGACTCC AAACTGAGCA ACCAGGTGTC CACCATCGTT 
AGCCTGCTGT CCACGCTCTG CCGGGGGTCG CCAGTGGTCA CTCATGACTT GTTACGTTCG 
GCACTCCCTA ACTCCATGGA GTCTGCGCTG GGAGGAGACG AGCGCTGTGT GCTGGACACC 
ATGCGGCTGG TCGACCTGCT GCTGGTGCTT CTGTTCGAGG GGCGGAAAGC GCTGCCAAAG 
TCCACCGCCG GCTCAGCAGG TCGGATCCCG GGGCTTCGCC GGCTGGACAG CTCAGGGGAG 
CGGTCGCACC GACAGCTCAT CGACTGCATC CGCAGCAAAG ACACGGACGC CCTGATAGAT 
GCCATCGACA CTGGAGCTTT TGAAGTGAAC TTCATGGACG ACGTGGGACA GACGCTGCTG 
AACTGGGCCT CAGCTTTTGG AACACAGGAA ATGGTGGAGT TTTTGTGCGA GAGGGGTGCA 
GATGTGAACC GAGGCCAGAG GTCATCCTCG CTGCACTACG CTGCCTGTTT CGGACGGCCA 
CAAGTTGCCA AGACGCTGCT GCGGCACGGA GCCAACCCGG ACCTGAGGGA TGAGGACGGG 
AAGACTCCTT TGGACAAAGC CAGGGAGAGG GGACACAGTG AGGTGGTGGC CATCCTTCAG 
TCTCCGGGAG ACTGGATGTG TCCAGTGAAC AAAGGAGACG ACAAGAGGAA GAAAGATGCG 
AGTAAGGAAG AAGAGGAGGG CAGTGAACCC AAAGGAGACC CAGAAATGGC TCCTTTCTAC 
CTGAAAAGAC TCCTCCCTGT TTTTGCACAA ACCTTTCAGC AAACCATGCT GCCTTCCATC 
AGGAAAGCCA GTCTGGCCCT CATCAGGAAG ATGGTTCACT ACAGCAGCGA GGTTCTGCTG 
AGGGAGGTGT GCGACAGTGA GACGGGACAC AACCTGCCCA CAGTGCTGGT GGAGATCACC 
GCCACCGTCC TCGACCAGGA GGATGATGAC GACGGTCACC TGCTGGCTCT GCAGATCATC 
AGGGATCTGG TGGACAAAGG TGGAGATGTT TTCCTTGACC AGCTGGCCCG ACTCGGGGTC 
ATCAACAAGG TGTCGACCTT GGCCGGACCG ACGTCTGATG ATGAGAACGA GGATGAAGTC 
AAACCTGAAA AGGAGGAGGA GGTGCAGGAA GATGCAAAGG AAATCCAGCA GGGGAAGCCG 
TACCACTGGA GGGACTGGTC CATCATCAGA GGCAGGGACT GCCTCTACAT TTGGTCAGAC 
GCCGCCGCCC TCGAGCTCTC CAACGGCTCC AATGGCTGGT TTCGCTTCAT CTTGGATGGG 
AAGCTCGCCA CCATGTACTC GAGTGGGAGC CCAGAGGGGG GGTCAGACAG CTCAGAGTCT 
CGCAGTGAGT TTTTGGAGAA GCTGCAGCGT GCCAGAAGTC AGGTGAAACC AGCAACAGCC 
AGCCAGCCCA TCCTCTCCAT GGTCGGACCC ACCAAGCTGA CGGTGGGGAA CTGGTCTCTG 
ACCTGCCTGA AGGAAGGCGA GATTGCAATT CACAACTCAG ACGGGCAGCA GGCCACGATC 
CTGAAGGAGG ATCTGCCCGG CTTCGTGTTC GAGTCCAACA GAGGAACCAA ACACTCGTTC 
ACTGCTGAAA CGTCGCTCGG TTCTGAGTTT GTCACTGGCT GGACGGGGAA ACGTGGCAGA 
AAGCTGAAGT CCAAGCTGGA GAAGACCAAG CAGAAGGTGA AGAGCATGGC CAGGGAGCTG 
TACGACGACC ACTTCAAAGC TGTGGAGAGC ATGCCCAGAG GAGTGGTGGT CACCCTGAGG 
AACATCGCCA CGCAGCTGGA GTCCGCCTGG GAGCTGCACA TAAACCGACA GTGTCTGGAA 
GGAGAGAACA CGTGGAGGGA CCTGATGAAG ACCGCTCTGG AGAACCTGAT TGTGGTTTTG 
AAGGATGAAA ACACGATTTC TCCGTATGAA ATGTGCAGCA GCGGCCTGGT TCAGGCTCTG 
TTTACCGTCC TCAGCAACAG TGTGGAGCTG GACCTGAAAC ATGACTGTAA GCCTCTAATG 
GAAAGGATCA ACGTCTTTAA AGCTGCTTTC AGCGAGAATG AAGACAATGA AAGCCGACCA 
GCCGTTGCCT TAGTGCGGAA GCTGATAGCC GTCCTGGAAT CGATCGAGCG TCTGCCCCTG 
CACCTGTATG ACACGCCTGG ATCCTCCTAC AATCTGCAGA TTCTGACGAG GAGGCTGCGG 
TTCCGTCTGG AGCGAGCGCC TGGGGAGACC GCTCTCATTG ACCGGACGGG ACGCATGCTG 
AAGATGGAGC CGCTCGCCAC CGTAGAGTCT CTGGAGCAGT ACCTGCTGAA AATGGTCGCT 
AAACAGTGGT ACGACTTTGA ACGCTCATCC TTCGTTTTTG TGAGAAAACT GAGAGAAGGA 
CAGATCTTCA CCTTCAGGCA CCAGCACGAC TTTGACGAGA ACGGCATCAT CTACTGGGTC 
GGAACCAACG CCAAGACGGC GTACGAGTGG GTGAACCCCG CAGCGTACGG CCTGGTGGTG 
GTGACCTCCT CCGAGGGGAG GAATCTGCCT TATGGGCGTT TGGAGGACAT CCTGAGTCGG 
GATAGCTCCG CCCTCAACTG CCACACGAAC GACGACAAGA ACGCCTGGTT CGCAGTTGAC 
CTCGGCCTCT GGGTGATTCC GTCAGCGTAC ACTCTGAGGC ACGCCAGGGG GTACGGACGC 
TCTGCGCTCA GGAACTGGGT TTTCCAAGTG TCCAAGGACG GACAGAACTG GTCCACACTC 
TACAACCACA TCGACGATGG CAGTCTCAAT GAACCGGGGT CTACGGCCAC GTGGCCTCTG 
GACCCGTCCA AAGAGGAGAA GCAGGGCTGG AGACACATCC GCATCAAGCA GATGGGGAAG 
AACGCCAGCG GTCAGACACA CTACCTGTCC TTATCTGGGC TGGAGCTGTA CGGCACGGTC 
ACCGCCGTAT GCGAGGACCA GCTGGGTAAA GCTGTGAAAG AGGCGGAGGC CAACCTTCGT 
CGCCAGCGGC GCCTCTTCCG TTCTCAGGTC ATGAAGTACA TCGTCCCAGG TGCGCGGGTC 
ATCCGTGGCA TCGACTGGAA GTGGAGGGAC CAGGATGGAA ACCCGGCAGG GGAAGGCACT 
GTCACTGGGG AGGCGCACAA CGGCTGGATT GATGTAACCT GGGATGCTGG CGGCTCTAAC 
TCTTATCGTA TGGGCGCCGA AGGGAAGTTT GACCTCAAGC TTGCTCCAGG GTACGACCCT 
GAGCTGGCTG CCACAGCGCC ATCACCCAAA CCTGTCTCAT CCGCTGTTTC AGGTCCCGCC 
TCCTCCACGG TGGGACCCTC TGCGACGCCA GCAGCTGTTG GCGGAACCAC CATCACCACC 
TCATCCTCCT CCACCACCTC ATCGTCCTCG CAGCAGCCTT CCTGGAGCAG TTTGGTGAAA 
AATAACTGTC CCGACAAGGG AGGGGCCAGC TCCTCCAGCA GGAAGGGTAG CAGCAGCTCC 
GTCTGCAGCG TCGCTTCCTC CTCTGACATC AGTCTGAGCT CCTCCATTGG GCTGCCTGGC 
GCTGGGAGCC TGCAGCTGGA CAGGAAGGCC GAGGGGCTGC TGCTAGATCA GGCCGCGGGG 
GTGGAAGGGG TGACGGGGGG AGGGACCGGT TCCGATGGAC ATCAGCAAGA GCCGATAGTC 
GTTCTGTCCT CCGTGACCGA ATCCGGTTCG GCGTCCAGCT CCGGCACGCT CACCGCCGAC 
GTGTCAACGG CGGGTGACGA GCATCACTCC CCCTCTGCAA TGGCCACTGC TGTGGACCCG 
GCCACGGCCA TCTCCATGGG CTTGGTGAGC GTCAGCTCCC CGGACGTCAG CTCCGTGTCG 
GAGCCCTCCA GCAAAGACAC TCACTCCCAG AGGCCGCTGT GCTCGGCCGT GAACACCCGG 
CTGTCCGTCA GCTCCCTGCT GGCCGCCAGC GCTCCCATGA GCTCCAGCGC CAGCGTGCCC 
AATCTGTCCT CTCGGGAGGC CAGCCTCATG GAGTCCTTCG TCCGCCGCGC GCCCAACATG 
TCCCGCACCA ACGCTACCAA CAACATGAAC CTGAGCCGCA GCAGCAGCGA CAACAACACC 
AACACACTGG GCAGGAACGT GATGAGCACA GCCACATCTC CTCTTATGGG CGCTCAGAGC 
TTTCCTAACC TCACCACCAC TGGCACCACC TCCACCGTTA CCATGTCAAC CTCCATAGTA 
ACCTGCGGTA ATAATGTAGC CACAGCCACC ACGGGGCTGT CTGTGGGACA GTTGCTAAGC 
AACACACTGA CAACCAGCTT AACCTCCACA TCCAGCGAGA GCGACACGGG ACAGGAGGCC 
GAGTTCTCCC TCTATGACTT CCTGGACAGT TGCCGGGCCA ACACGCTGCT AGCAGAGCTG 
GACGATGAGG AGGACCTCCC AGAGCCCGAC GACGACGACG ATGAGAATGA AGACGACAAT 
CAGGAGGATC AGGAGTATGA GGAGGTCCTG GAGGAGGAGG AGTATGAGAC GAAGGGAGGA 
CGGAGGAGGA CATGGGACGA TGACTTTGTC CTAAAAAGAC AGTTTTCTGC TTTGGTCCCT 
GCCTTCGACC CCAGACCAGG AAGAACCAAC GTCCAGCAGA CCACCGACCT GGAGATCCCC 
CCACCAGGAA CTCCGCGGTC GGAGGTTCGG GAGGAGGTGG AGTGTGCCCC ATCCCCCCAC 
CTCGCCCTCA CACTGAAGGT GGCGGGTCTC GGCACGGCTC GGGAGGTGGA GATCCCGCTC 
TCCAACTACA AGTTGACCAT CTTCTACTAT GTGCAGCGGC TCCTGCAGCT CTCCTGCAAC 
GGCGCCGTAA AGACGGACAA GCTGCGGCGC ATCTGGGAGC CGACGTACAC GATCATGTAC 
AGAGAGCTCA AAGACTCGGA CAAAGAGAAG GAGAGCGGGA AGATGGACCT CTTTGAGCAC 
GTTGTTGGAA GTCGTTCTGG AGGTCTGAGC CCCAGCTCGG GTTCAGCCAA TCAGAGCAGC 
GAGATCCTGG GCTGGGCGAA GGAGGCGGTC CTGGCCAAGG CGGGCTGCAG CCAGAACGCC 
TGCGGGGTGG AAGACGTCCT GCAGCTGCTG CGCATTCTCT ATGTGATTGG GAGAGACTCT 
ACTGCAGGCG GACGCACGCT GCAAGAGGAT GTTGACGAGC TGCAGTTCAA CTCGCCTCCT 
GAGGAGTTCA GCAGCAAGAA GATCACCACC AAGATCCTGC AGCAGATCGA GGAGCCCTTG 
GCTCTGGCTA GCGGAGCGCT GCCGGACTGG TGTGAGCAGC TCACCTCCAA GTGTCCTTTC 
CTGATCCCCT TTGAGACCAG ACAGCTGTAC TTCACCTGCA CTGCCTTTGG GGCCTCCAGG 
GCGATCGTGT GGCTCCAGAA CCGCCGCGAG GCGACCATGG AGCGCTCACG GCCATCCACC 
ACCGTCCGGC GAGACGACCC CGGAGAGTTC AGAGTCGGTC GGCTTAAACA CGAGCGCGTT 
AAAGTTCCTC GAGGGGAGTC CATGATGGAG TGGGCGGAGT CTGTGATGCA GATCCACGCA 
AACAGGAAGT CTGTTTTAGA GGTGGAGTTT CTGGGTGAGG AGGGGACGGG TCTGGGTCCG 
ACTCTGGAGT TCTACGCTCT GGTGGCGGCG GAGTTTCAGA GAACGTCTCT GGGGATCTGG 
CTCTGTGACG ACGACTTCCC GGACGACGAG TCCCGACAGG TGGATCTGGG TGGAGGTCTG 
AAGCCTCCTG GCTTCTACGT GCAGCGGTCC TGCGGGCTCT TCCCGGCGCC GTTCCCTCAG 
GACAGCGAGG AGCTGGAGCG GATTACGCGG CTCTTCCACT TCCTGGGGAT CTTCCTGGCC 
AAGTGCATCC AGGACAACCG GCTGGTGGAC CTGCCCGTGT CCCAGCCTTT CTTCAAGCTG 
CTCTGCATGG GGGACATCAA GTCCAACATG AGCAAGCTGC TGTACCAGTC CCGCGGCTCC 
CCCCAGGGCC ACCTCTCCGA GAGGGCCCCC CTGCTGCTGC TGACGGAGGT GCAGTCGGAG 
GCGTCCACAG AGGAGAGCCA GGAGACCTAC TCTGTGGGGA GCTTCGACGA GGACTCCAAG 
TCAGACTTCA TCATGGACCC CCCCAAACCC AAGCCGCCCG CCTGGTTCCA CGGCATCATG 
TCCTGGGACG ACTTCCATCT GGTCAACCCG CACAGAGCCA GTTTCCTGAA GGAGGTGAAG 
GAGCTGGCAG TGAGGAGGAG ACAGATTCTG ACCAGCAAGA GTTTGTCAGA GGATGAGAAG 
AACACCAGGC TGCAGGACCT GATGCTGAGG AACCCGCTGG GCTCTGGGCC CCCCTTAAGC 
GTCGAAGACC TCGGGTTGAA CTTCCAGTTC TGTCCGTCCT CCAAGATTCA CGGCTTCTTC 
GCGGTGGATC TGAAACCAAA CGGGGATGAC GAGATGGTGA GTCTGGAGAA CGCCGAGGAG 
TACGTGGAGC TGATGTTCGA CTTCTGCATG CACGCCGGCA TCCAGAAGCA GATGGAGGCG 
TTCAGAGAGG GTTTCAACCG CGTCTTCCCC ATGGAGAAGC TCAGCTCGTT CAGCCAGAAG 
GAGGTGCAGA TGATCCTCTG CGGAAACCAA TCCCCCTGCT GGACCGCCGA TGACATCATC 
AGCTACACCG AGCCAAAGCT GGGATACACC AGAGACAGCC CTGGGTTCCT GCGCTTCATC 
CGAGTTTTAT GCGGAATGTC GACGGACGAG AGGAAAGCTT TCCTGCAGTT CACCACCGGC 
TGCTCCACGC TGCCCCCCGG TGGCCTGGCT AACCTGCACC CGCGCCTCAC CATCGTCAGA 
AAGGTGGACG CCACGGACTC CAGCTACCCA TCGGTGAACA CGTGCGTTCA CTACCTGAAG 
CTGCCGGAGT ACTCGTCAGA GGAGATCATG AGGGAGCGGC TGTTGGCGGC CACCATGGAG 
AAGGGCTTCC ACCTCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011488438.1
CDS	360..8060
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEASGLNCVLSFIRDSGHLVHKDTLHSAMAVVSRLCSKMEPQDSSLETCVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGSVSGSSSACKPGRPSTGATPSASDSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSALPNSMESALGGDERCVLDTMRLVDLLLVLLFEGRKALPKSTAGSAGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKRKKDASKEEEEGSEPKGDPEMAPFYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMVHYSSEVLLREVCDSETGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDVFLDQLARLGVINKVSTLAGPTSDDENEDEVKPEKEEEVQEDAKEIQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARSQVKPATASQPILSMVGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVKSMARELYDDHFKAVESMPRGVVVTLRNIATQLESAWELHINRQCLEGENTWRDLMKTALENLIVVLKDENTISPYEMCSSGLVQALFTVLSNSVELDLKHDCKPLMERINVFKAAFSENEDNESRPAVALVRKLIAVLESIERLPLHLYDTPGSSYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFERSSFVFVRKLREGQIFTFRHQHDFDENGIIYWVGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDSSALNCHTNDDKNAWFAVDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWSTLYNHIDDGSLNEPGSTATWPLDPSKEEKQGWRHIRIKQMGKNASGQTHYLSLSGLELYGTVTAVCEDQLGKAVKEAEANLRRQRRLFRSQVMKYIVPGARVIRGIDWKWRDQDGNPAGEGTVTGEAHNGWIDVTWDAGGSNSYRMGAEGKFDLKLAPGYDPELAATAPSPKPVSSAVSGPASSTVGPSATPAAVGGTTITTSSSSTTSSSSQQPSWSSLVKNNCPDKGGASSSSRKGSSSSVCSVASSSDISLSSSIGLPGAGSLQLDRKAEGLLLDQAAGVEGVTGGGTGSDGHQQEPIVVLSSVTESGSASSSGTLTADVSTAGDEHHSPSAMATAVDPATAISMGLVSVSSPDVSSVSEPSSKDTHSQRPLCSAVNTRLSVSSLLAASAPMSSSASVPNLSSREASLMESFVRRAPNMSRTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTTGTTSTVTMSTSIVTCGNNVATATTGLSVGQLLSNTLTTSLTSTSSESDTGQEAEFSLYDFLDSCRANTLLAELDDEEDLPEPDDDDDENEDDNQEDQEYEEVLEEEEYETKGGRRRTWDDDFVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPRSEVREEVECAPSPHLALTLKVAGLGTAREVEIPLSNYKLTIFYYVQRLLQLSCNGAVKTDKLRRIWEPTYTIMYRELKDSDKEKESGKMDLFEHVVGSRSGGLSPSSGSANQSSEILGWAKEAVLAKAGCSQNACGVEDVLQLLRILYVIGRDSTAGGRTLQEDVDELQFNSPPEEFSSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATMERSRPSTTVRRDDPGEFRVGRLKHERVKVPRGESMMEWAESVMQIHANRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTSLGIWLCDDDFPDDESRQVDLGGGLKPPGFYVQRSCGLFPAPFPQDSEELERITRLFHFLGIFLAKCIQDNRLVDLPVSQPFFKLLCMGDIKSNMSKLLYQSRGSPQGHLSERAPLLLLTEVQSEASTEESQETYSVGSFDEDSKSDFIMDPPKPKPPAWFHGIMSWDDFHLVNPHRASFLKEVKELAVRRRQILTSKSLSEDEKNTRLQDLMLRNPLGSGPPLSVEDLGLNFQFCPSSKIHGFFAVDLKPNGDDEMVSLENAEEYVELMFDFCMHAGIQKQMEAFREGFNRVFPMEKLSSFSQKEVQMILCGNQSPCWTADDIISYTEPKLGYTRDSPGFLRFIRVLCGMSTDERKAFLQFTTGCSTLPPGGLANLHPRLTIVRKVDATDSSYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_011490136.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes HECT domain E3 ubiquitin protein ligase 1 (hectd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011490136.2

ORF Insert Sequence:

ATGGCGGATG TGGACCCAGA CACGTTGCTG GAGTGGCTGC AGATGGGACA GGGCGATGAG 
CGGGACATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGCTGCTCAT GTCGGACAAC 
GTGGACCGCT GCTTTGAGAC ATGTCCTCCT CGAACGTTCC TCCCAGCGCT TTGTAAGATC 
TTCTTGGACG AGAGCGCTCC TGATAACGTG CTGGAGGTCA CGGCCAGAGC CATCACCTAC 
TACCTGGACG TGTCGGCAGA ATGCACGCGG AGGATCGTGG GTGTGGACGG GGCCATAAAG 
GCCCTGTGCA ACCGGCTGGT GGTGGTGGAA CTCAACAACA GGACCAGCAG GGATCTGGCT 
GAGCAGTGTG TTAAGGTGCT GGAGCTGATC TGTACCAGGG AATCTGGCGC CGTGTTTGAA 
GCCAGCGGTC TGAACTGCGT TTTGAGCTTC ATACGCGACA GTGGTCACCT GGTCCACAAA 
GACACTCTGC ACTCTGCAAT GGCAGTGGTG TCTCGCCTCT GTAGCAAGAT GGAGCCGCAG 
GACTCTTCTC TAGAAACCTG CGTGGAGTCT CTGTCCAGCC TCCTGAAACA TGAGGATCAC 
CAGGTGTCAG ACGGTGCTCT GCGTTGCTTC GCCTCATTGG CTGATCGGTT TACTCGTCGA 
GGCGTAGACC CCGCCCCTCT GGCCAAACAT GGACTGACGG AGGAGCTGCT TTCCCGCATG 
GCGGCTGCCG GAGGTTCCGT GTCGGGCTCT TCTTCTGCCT GTAAACCAGG TCGGCCCTCC 
ACTGGTGCCA CACCGTCTGC CTCCGACTCC AAACTGAGCA ACCAGGTGTC CACCATCGTT 
AGCCTGCTGT CCACGCTCTG CCGGGGGTCG CCAGTGGTCA CTCATGACTT GTTACGTTCG 
GCACTCCCTA ACTCCATGGA GTCTGCGCTG GGAGGAGACG AGCGCTGTGT GCTGGACACC 
ATGCGGCTGG TCGACCTGCT GCTGGTGCTT CTGTTCGAGG GGCGGAAAGC GCTGCCAAAG 
TCCACCGCCG GCTCAGCAGG TCGGATCCCG GGGCTTCGCC GGCTGGACAG CTCAGGGGAG 
CGGTCGCACC GACAGCTCAT CGACTGCATC CGCAGCAAAG ACACGGACGC CCTGATAGAT 
GCCATCGACA CTGGAGCTTT TGAAGTGAAC TTCATGGACG ACGTGGGACA GACGCTGCTG 
AACTGGGCCT CAGCTTTTGG AACACAGGAA ATGGTGGAGT TTTTGTGCGA GAGGGGTGCA 
GATGTGAACC GAGGCCAGAG GTCATCCTCG CTGCACTACG CTGCCTGTTT CGGACGGCCA 
CAAGTTGCCA AGACGCTGCT GCGGCACGGA GCCAACCCGG ACCTGAGGGA TGAGGACGGG 
AAGACTCCTT TGGACAAAGC CAGGGAGAGG GGACACAGTG AGGTGGTGGC CATCCTTCAG 
TCTCCGGGAG ACTGGATGTG TCCAGTGAAC AAAGGAGACG ACAAGAGGAA GAAAGATGCG 
AGTAAGGAAG AAGAGGAGGG CAGTGAACCC AAAGGAGACC CAGAAATGGC TCCTTTCTAC 
CTGAAAAGAC TCCTCCCTGT TTTTGCACAA ACCTTTCAGC AAACCATGCT GCCTTCCATC 
AGGAAAGCCA GTCTGGCCCT CATCAGGAAG ATGGTTCACT ACAGCAGCGA GGTTCTGCTG 
AGGGAGGTGT GCGACAGTGA GACGGGACAC AACCTGCCCA CAGTGCTGGT GGAGATCACC 
GCCACCGTCC TCGACCAGGA GGATGATGAC GACGGTCACC TGCTGGCTCT GCAGATCATC 
AGGGATCTGG TGGACAAAGG TGGAGATGTT TTCCTTGACC AGCTGGCCCG ACTCGGGGTC 
ATCAACAAGG TGTCGACCTT GGCCGGACCG ACGTCTGATG ATGAGAACGA GGATGAAGTC 
AAACCTGAAA AGGAGGAGGA GGTGCAGGAA GATGCAAAGG AAATCCAGCA GGGGAAGCCG 
TACCACTGGA GGGACTGGTC CATCATCAGA GGCAGGGACT GCCTCTACAT TTGGTCAGAC 
GCCGCCGCCC TCGAGCTCTC CAACGGCTCC AATGGCTGGT TTCGCTTCAT CTTGGATGGG 
AAGCTCGCCA CCATGTACTC GAGTGGGAGC CCAGAGGGGG GGTCAGACAG CTCAGAGTCT 
CGCAGTGAGT TTTTGGAGAA GCTGCAGCGT GCCAGAAGTC AGGTGAAACC AGCAACAGCC 
AGCCAGCCCA TCCTCTCCAT GGTCGGACCC ACCAAGCTGA CGGTGGGGAA CTGGTCTCTG 
ACCTGCCTGA AGGAAGGCGA GATTGCAATT CACAACTCAG ACGGGCAGCA GGCCACGATC 
CTGAAGGAGG ATCTGCCCGG CTTCGTGTTC GAGTCCAACA GAGGAACCAA ACACTCGTTC 
ACTGCTGAAA CGTCGCTCGG TTCTGAGTTT GTCACTGGCT GGACGGGGAA ACGTGGCAGA 
AAGCTGAAGT CCAAGCTGGA GAAGACCAAG CAGAAGGTGA AGAGCATGGC CAGGGAGCTG 
TACGACGACC ACTTCAAAGC TGTGGAGAGC ATGCCCAGAG GAGTGGTGGT CACCCTGAGG 
AACATCGCCA CGCAGCTGGA GTCCGCCTGG GAGCTGCACA TAAACCGACA GTGTCTGGAA 
GGAGAGAACA CGTGGAGGGA CCTGATGAAG ACCGCTCTGG AGAACCTGAT TGTGGTTTTG 
AAGGATGAAA ACACGATTTC TCCGTATGAA ATGTGCAGCA GCGGCCTGGT TCAGGCTCTG 
TTTACCGTCC TCAGCAACAG TGTGGAGCTG GACCTGAAAC ATGACTGTAA GCCTCTAATG 
GAAAGGATCA ACGTCTTTAA AGCTGCTTTC AGCGAGAATG AAGACAATGA AAGCCGACCA 
GCCGTTGCCT TAGTGCGGAA GCTGATAGCC GTCCTGGAAT CGATCGAGCG TCTGCCCCTG 
CACCTGTATG ACACGCCTGG ATCCTCCTAC AATCTGCAGA TTCTGACGAG GAGGCTGCGG 
TTCCGTCTGG AGCGAGCGCC TGGGGAGACC GCTCTCATTG ACCGGACGGG ACGCATGCTG 
AAGATGGAGC CGCTCGCCAC CGTAGAGTCT CTGGAGCAGT ACCTGCTGAA AATGGTCGCT 
AAACAGTGGT ACGACTTTGA ACGCTCATCC TTCGTTTTTG TGAGAAAACT GAGAGAAGGA 
CAGATCTTCA CCTTCAGGCA CCAGCACGAC TTTGACGAGA ACGGCATCAT CTACTGGGTC 
GGAACCAACG CCAAGACGGC GTACGAGTGG GTGAACCCCG CAGCGTACGG CCTGGTGGTG 
GTGACCTCCT CCGAGGGGAG GAATCTGCCT TATGGGCGTT TGGAGGACAT CCTGAGTCGG 
GATAGCTCCG CCCTCAACTG CCACACGAAC GACGACAAGA ACGCCTGGTT CGCAGTTGAC 
CTCGGCCTCT GGGTGATTCC GTCAGCGTAC ACTCTGAGGC ACGCCAGGGG GTACGGACGC 
TCTGCGCTCA GGAACTGGGT TTTCCAAGTG TCCAAGGACG GACAGAACTG GTCCACACTC 
TACAACCACA TCGACGATGG CAGTCTCAAT GAACCGGGGT CTACGGCCAC GTGGCCTCTG 
GACCCGTCCA AAGAGGAGAA GCAGGGCTGG AGACACATCC GCATCAAGCA GATGGGGAAG 
AACGCCAGCG GTCAGACACA CTACCTGTCC TTATCTGGGC TGGAGCTGTA CGGCACGGTC 
ACCGCCGTAT GCGAGGACCA GCTGGGTAAA GCTGTGAAAG AGGCGGAGGC CAACCTTCGT 
CGCCAGCGGC GCCTCTTCCG TTCTCAGGTC ATGAAGTACA TCGTCCCAGG TGCGCGGGTC 
ATCCGTGGCA TCGACTGGAA GTGGAGGGAC CAGGATGGAA ACCCGGCAGG GGAAGGCACT 
GTCACTGGGG AGGCGCACAA CGGCTGGATT GATGTAACCT GGGATGCTGG CGGCTCTAAC 
TCTTATCGTA TGGGCGCCGA AGGGAAGTTT GACCTCAAGC TTGCTCCAGG GTACGACCCT 
GAGCTGGCTG CCACAGCGCC ATCACCCAAA CCTGTCTCAT CCGCTGTTTC AGGTCCCGCC 
TCCTCCACGG TGGGACCCTC TGCGACGCCA GCAGCTGTTG GCGGAACCAC CATCACCACC 
TCATCCTCCT CCACCACCTC ATCGTCCTCG CAGCAGCCTT CCTGGAGCAG TTTGGTGAAA 
AATAACTGTC CCGACAAGGG AGGGGCCAGC TCCTCCAGCA GGAAGGGTAG CAGCAGCTCC 
GTCTGCAGCG TCGCTTCCTC CTCTGACATC AGTCTGAGCT CCTCCATTGG GCTGCCTGGC 
GCTGGGAGCC TGCAGCTGGA CAGGAAGGCC GAGGGGCTGC TGCTAGATCA GGCCGCGGGG 
GTGGAAGGGG TGACGGGGGG AGGGACCGGT TCCGATGGAC ATCAGCAAGA GCCGATAGTC 
GTTCTGTCCT CCGTGACCGA ATCCGGTTCG GCGTCCAGCT CCGGCACGCT CACCGCCGAC 
GTGTCAACGG CGGGTGACGA GCATCACTCC CCCTCTGCAA TGGCCACTGC TGTGGACCCG 
GCCACGGCCA TCTCCATGGG CTTGGTGAGC GTCAGCTCCC CGGACGTCAG CTCCGTGTCG 
GAGCCCTCCA GCAAAGACAC TCACTCCCAG AGGCCGCTGT GCTCGGCCGT GAACACCCGG 
CTGTCCGTCA GCTCCCTGCT GGCCGCCAGC GCTCCCATGA GCTCCAGCGC CAGCGTGCCC 
AATCTGTCCT CTCGGGAGGC CAGCCTCATG GAGTCCTTCG TCCGCCGCGC GCCCAACATG 
TCCCGCACCA ACGCTACCAA CAACATGAAC CTGAGCCGCA GCAGCAGCGA CAACAACACC 
AACACACTGG GCAGGAACGT GATGAGCACA GCCACATCTC CTCTTATGGG CGCTCAGAGC 
TTTCCTAACC TCACCACCAC TGGCACCACC TCCACCGTTA CCATGTCAAC CTCCATAGTA 
ACCTGCGGTA ATAATGTAGC CACAGCCACC ACGGGGCTGT CTGTGGGACA GTTGCTAAGC 
AACACACTGA CAACCAGCTT AACCTCCACA TCCAGCGAGA GCGACACGGG ACAGGAGGCC 
GAGTTCTCCC TCTATGACTT CCTGGACAGT TGCCGGGCCA ACACGCTGCT AGCAGAGCTG 
GACGATGAGG AGGACCTCCC AGAGCCCGAC GACGACGACG ATGAGAATGA AGACGACAAT 
CAGGAGGATC AGGAGTATGA GGAGGTCCTG GAGGAGGAGG AGTATGAGAC GAAGGGAGGA 
CGGAGGAGGA CATGGGACGA TGACTTTGTC CTAAAAAGAC AGTTTTCTGC TTTGGTCCCT 
GCCTTCGACC CCAGACCAGG AAGAACCAAC GTCCAGCAGA CCACCGACCT GGAGATCCCC 
CCACCAGGAA CTCCGCGGTC GGAGGTTCGG GAGGAGGTGG AGTGTGCCCC ATCCCCCCAC 
CTCGCCCTCA CACTGAAGGT GGCGGGTCTC GGCACGGCTC GGGAGGTGGA GATCCCGCTC 
TCCAACTACA AGTTGACCAT CTTCTACTAT GTGCAGCGGC TCCTGCAGCT CTCCTGCAAC 
GGCGCCGTAA AGACGGACAA GCTGCGGCGC ATCTGGGAGC CGACGTACAC GATCATGTAC 
AGAGAGCTCA AAGACTCGGA CAAAGAGAAG GAGAGCGGGA AGATGGACCT CTTTGAGCAC 
GTTGTTGGAA GTCGTTCTGG AGGTCTGAGC CCCAGCTCGG GTTCAGCCAA TCAGAGCAGC 
GAGATCCTGG GCTGGGCGAA GGAGGCGGTC CTGGCCAAGG CGGGCTGCAG CCAGAACGCC 
TGCGGGGTGG AAGACGTCCT GCAGCTGCTG CGCATTCTCT ATGTGATTGG GAGAGACTCT 
ACTGCAGGCG GACGCACGCT GCAAGAGGAT GTTGACGAGC TGCAGTTCAA CTCGCCTCCT 
GAGGAGTTCA GCAGCAAGAA GATCACCACC AAGATCCTGC AGCAGATCGA GGAGCCCTTG 
GCTCTGGCTA GCGGAGCGCT GCCGGACTGG TGTGAGCAGC TCACCTCCAA GTGTCCTTTC 
CTGATCCCCT TTGAGACCAG ACAGCTGTAC TTCACCTGCA CTGCCTTTGG GGCCTCCAGG 
GCGATCGTGT GGCTCCAGAA CCGCCGCGAG GCGACCATGG AGCGCTCACG GCCATCCACC 
ACCGTCCGGC GAGACGACCC CGGAGAGTTC AGAGTCGGTC GGCTTAAACA CGAGCGCGTT 
AAAGTTCCTC GAGGGGAGTC CATGATGGAG TGGGCGGAGT CTGTGATGCA GATCCACGCA 
AACAGGAAGT CTGTTTTAGA GGTGGAGTTT CTGGGTGAGG AGGGGACGGG TCTGGGTCCG 
ACTCTGGAGT TCTACGCTCT GGTGGCGGCG GAGTTTCAGA GAACGTCTCT GGGGATCTGG 
CTCTGTGACG ACGACTTCCC GGACGACGAG TCCCGACAGG TGGATCTGGG TGGAGGTCTG 
AAGCCTCCTG GCTTCTACGT GCAGCGGTCC TGCGGGCTCT TCCCGGCGCC GTTCCCTCAG 
GACAGCGAGG AGCTGGAGCG GATTACGCGG CTCTTCCACT TCCTGGGGAT CTTCCTGGCC 
AAGTGCATCC AGGACAACCG GCTGGTGGAC CTGCCCGTGT CCCAGCCTTT CTTCAAGCTG 
CTCTGCATGG GGGACATCAA GTCCAACATG AGCAAGCTGC TGTACCAGTC CCGCGGCTCC 
CCCCAGGGCC ACCTCTCCGA GAGGGCCCCC CTGCTGCTGC TGACGGAGGT GCAGTCGGAG 
GCGTCCACAG AGGAGAGCCA GGAGACCTAC TCTGTGGGGA GCTTCGACGA GGACTCCAAG 
TCAGACTTCA TCATGGACCC CCCCAAACCC AAGCCGCCCG CCTGGTTCCA CGGCATCATG 
TCCTGGGACG ACTTCCATCT GGTCAACCCG CACAGAGCCA GTTTCCTGAA GGAGGTGAAG 
GAGCTGGCAG TGAGGAGGAG ACAGATTCTG ACCAGCAAGA GTTTGTCAGA GGATGAGAAG 
AACACCAGGC TGCAGGACCT GATGCTGAGG AACCCGCTGG GCTCTGGGCC CCCCTTAAGC 
GTCGAAGACC TCGGGTTGAA CTTCCAGTTC TGTCCGTCCT CCAAGATTCA CGGCTTCTTC 
GCGGTGGATC TGAAACCAAA CGGGGATGAC GAGATGGTGA GTCTGGAGAA CGCCGAGGAG 
TACGTGGAGC TGATGTTCGA CTTCTGCATG CACGCCGGCA TCCAGAAGCA GATGGAGGCG 
TTCAGAGAGG GTTTCAACCG CGTCTTCCCC ATGGAGAAGC TCAGCTCGTT CAGCCAGAAG 
GAGGTGCAGA TGATCCTCTG CGGAAACCAA TCCCCCTGCT GGACCGCCGA TGACATCATC 
AGCTACACCG AGCCAAAGCT GGGATACACC AGAGACAGCC CTGGGTTCCT GCGCTTCATC 
CGAGTTTTAT GCGGAATGTC GACGGACGAG AGGAAAGCTT TCCTGCAGTT CACCACCGGC 
TGCTCCACGC TGCCCCCCGG TGGCCTGGCT AACCTGCACC CGCGCCTCAC CATCGTCAGA 
AAGGTGGACG CCACGGACTC CAGCTACCCA TCGGTGAACA CGTGCGTTCA CTACCTGAAG 
CTGCCGGAGT ACTCGTCAGA GGAGATCATG AGGGAGCGGC TGTTGGCGGC CACCATGGAG 
AAGGGCTTCC ACCTCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hectd1 ( XM_011490137.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011488439.1 Oryzias latipes HECT domain E3 ubiquitin protein ligase 1 (hectd1), transcript variant X2, mRNA.

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CloneID	OJa31518
Clone ID Related Accession (Same CDS sequence)	XM_011490137.2 , XM_011490137.3
Accession Version	XM_011490137.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011490137.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG TGGACCCAGA CACGTTGCTG GAGTGGCTGC AGATGGGACA GGGCGATGAG 
CGGGACATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGCTGCTCAT GTCGGACAAC 
GTGGACCGCT GCTTTGAGAC ATGTCCTCCT CGAACGTTCC TCCCAGCGCT TTGTAAGATC 
TTCTTGGACG AGAGCGCTCC TGATAACGTG CTGGAGGTCA CGGCCAGAGC CATCACCTAC 
TACCTGGACG TGTCGGCAGA ATGCACGCGG AGGATCGTGG GTGTGGACGG GGCCATAAAG 
GCCCTGTGCA ACCGGCTGGT GGTGGTGGAA CTCAACAACA GGACCAGCAG GGATCTGGCT 
GAGCAGTGTG TTAAGGTGCT GGAGCTGATC TGTACCAGGG AATCTGGCGC CGTGTTTGAA 
GCCAGCGGTC TGAACTGCGT TTTGAGCTTC ATACGCGACA GTGGTCACCT GGTCCACAAA 
GACACTCTGC ACTCTGCAAT GGCAGTGGTG TCTCGCCTCT GTAGCAAGAT GGAGCCGCAG 
GACTCTTCTC TAGAAACCTG CGTGGAGTCT CTGTCCAGCC TCCTGAAACA TGAGGATCAC 
CAGGTGTCAG ACGGTGCTCT GCGTTGCTTC GCCTCATTGG CTGATCGGTT TACTCGTCGA 
GGCGTAGACC CCGCCCCTCT GGCCAAACAT GGACTGACGG AGGAGCTGCT TTCCCGCATG 
GCGGCTGCCG GAGGTTCCGT GTCGGGCTCT TCTTCTGCCT GTAAACCAGG TCGGCCCTCC 
ACTGGTGCCA CACCGTCTGC CTCCGACTCC AAACTGAGCA ACCAGGTGTC CACCATCGTT 
AGCCTGCTGT CCACGCTCTG CCGGGGGTCG CCAGTGGTCA CTCATGACTT GTTACGTTCG 
GCACTCCCTA ACTCCATGGA GTCTGCGCTG GGAGGAGACG AGCGCTGTGT GCTGGACACC 
ATGCGGCTGG TCGACCTGCT GCTGGTGCTT CTGTTCGAGG GGCGGAAAGC GCTGCCAAAG 
TCCACCGCCG GCTCAGCAGG TCGGATCCCG GGGCTTCGCC GGCTGGACAG CTCAGGGGAG 
CGGTCGCACC GACAGCTCAT CGACTGCATC CGCAGCAAAG ACACGGACGC CCTGATAGAT 
GCCATCGACA CTGGAGCTTT TGAAGTGAAC TTCATGGACG ACGTGGGACA GACGCTGCTG 
AACTGGGCCT CAGCTTTTGG AACACAGGAA ATGGTGGAGT TTTTGTGCGA GAGGGGTGCA 
GATGTGAACC GAGGCCAGAG GTCATCCTCG CTGCACTACG CTGCCTGTTT CGGACGGCCA 
CAAGTTGCCA AGACGCTGCT GCGGCACGGA GCCAACCCGG ACCTGAGGGA TGAGGACGGG 
AAGACTCCTT TGGACAAAGC CAGGGAGAGG GGACACAGTG AGGTGGTGGC CATCCTTCAG 
TCTCCGGGAG ACTGGATGTG TCCAGTGAAC AAAGGAGACG ACAAGAGGAA GAAAGATGCG 
AGTAAGGAAG AAGAGGAGGG CAGTGAACCC AAAGGAGACC CAGAAATGGC TCCTTTCTAC 
CTGAAAAGAC TCCTCCCTGT TTTTGCACAA ACCTTTCAGC AAACCATGCT GCCTTCCATC 
AGGAAAGCCA GTCTGGCCCT CATCAGGAAG ATGGTTCACT ACAGCAGCGA GGTTCTGCTG 
AGGGAGGTGT GCGACAGTGA GACGGGACAC AACCTGCCCA CAGTGCTGGT GGAGATCACC 
GCCACCGTCC TCGACCAGGA GGATGATGAC GACGGTCACC TGCTGGCTCT GCAGATCATC 
AGGGATCTGG TGGACAAAGG TGGAGATGTT TTCCTTGACC AGCTGGCCCG ACTCGGGGTC 
ATCAACAAGG TGTCGACCTT GGCCGGACCG ACGTCTGATG ATGAGAACGA GGATGAAGTC 
AAACCTGAAA AGGAGGAGGA GGTGCAGGAA GATGCAAAGG AAATCCAGCA GGGGAAGCCG 
TACCACTGGA GGGACTGGTC CATCATCAGA GGCAGGGACT GCCTCTACAT TTGGTCAGAC 
GCCGCCGCCC TCGAGCTCTC CAACGGCTCC AATGGCTGGT TTCGCTTCAT CTTGGATGGG 
AAGCTCGCCA CCATGTACTC GAGTGGGAGC CCAGAGGGGG GGTCAGACAG CTCAGAGTCT 
CGCAGTGAGT TTTTGGAGAA GCTGCAGCGT GCCAGAAGTC AGGTGAAACC AGCAACAGCC 
AGCCAGCCCA TCCTCTCCAT GGTCGGACCC ACCAAGCTGA CGGTGGGGAA CTGGTCTCTG 
ACCTGCCTGA AGGAAGGCGA GATTGCAATT CACAACTCAG ACGGGCAGCA GGCCACGATC 
CTGAAGGAGG ATCTGCCCGG CTTCGTGTTC GAGTCCAACA GAGGAACCAA ACACTCGTTC 
ACTGCTGAAA CGTCGCTCGG TTCTGAGTTT GTCACTGGCT GGACGGGGAA ACGTGGCAGA 
AAGCTGAAGT CCAAGCTGGA GAAGACCAAG CAGAAGGTGA AGAGCATGGC CAGGGAGCTG 
TACGACGACC ACTTCAAAGC TGTGGAGAGC ATGCCCAGAG GAGTGGTGGT CACCCTGAGG 
AACATCGCCA CGCAGCTGGA GTCCGCCTGG GAGCTGCACA TAAACCGACA GTGTCTGGAA 
GGAGAGAACA CGTGGAGGGA CCTGATGAAG ACCGCTCTGG AGAACCTGAT TGTGGTTTTG 
AAGGATGAAA ACACGATTTC TCCGTATGAA ATGTGCAGCA GCGGCCTGGT TCAGGCTCTG 
TTTACCGTCC TCAGCAACAG TGTGGAGCTG GACCTGAAAC ATGACTGTAA GCCTCTAATG 
GAAAGGATCA ACGTCTTTAA AGCTGCTTTC AGCGAGAATG AAGACAATGA AAGCCGACCA 
GCCGTTGCCT TAGTGCGGAA GCTGATAGCC GTCCTGGAAT CGATCGAGCG TCTGCCCCTG 
CACCTGTATG ACACGCCTGG ATCCTCCTAC AATCTGCAGA TTCTGACGAG GAGGCTGCGG 
TTCCGTCTGG AGCGAGCGCC TGGGGAGACC GCTCTCATTG ACCGGACGGG ACGCATGCTG 
AAGATGGAGC CGCTCGCCAC CGTAGAGTCT CTGGAGCAGT ACCTGCTGAA AATGGTCGCT 
AAACAGTGGT ACGACTTTGA ACGCTCATCC TTCGTTTTTG TGAGAAAACT GAGAGAAGGA 
CAGATCTTCA CCTTCAGGCA CCAGCACGAC TTTGACGAGA ACGGCATCAT CTACTGGGTC 
GGAACCAACG CCAAGACGGC GTACGAGTGG GTGAACCCCG CAGCGTACGG CCTGGTGGTG 
GTGACCTCCT CCGAGGGGAG GAATCTGCCT TATGGGCGTT TGGAGGACAT CCTGAGTCGG 
GATAGCTCCG CCCTCAACTG CCACACGAAC GACGACAAGA ACGCCTGGTT CGCAGTTGAC 
CTCGGCCTCT GGGTGATTCC GTCAGCGTAC ACTCTGAGGC ACGCCAGGGG GTACGGACGC 
TCTGCGCTCA GGAACTGGGT TTTCCAAGTG TCCAAGGACG GACAGAACTG GTCCACACTC 
TACAACCACA TCGACGATGG CAGTCTCAAT GAACCGGGGT CTACGGCCAC GTGGCCTCTG 
GACCCGTCCA AAGAGGAGAA GCAGGGCTGG AGACACATCC GCATCAAGCA GATGGGGAAG 
AACGCCAGCG GTCAGACACA CTACCTGTCC TTATCTGGGC TGGAGCTGTA CGGCACGGTC 
ACCGCCGTAT GCGAGGACCA GCTGGGTAAA GCTGTGAAAG AGGCGGAGGC CAACCTTCGT 
CGCCAGCGGC GCCTCTTCCG TTCTCAGGTC ATGAAGTACA TCGTCCCAGG TGCGCGGGTC 
ATCCGTGGCA TCGACTGGAA GTGGAGGGAC CAGGATGGAA ACCCGGCAGG GGAAGGCACT 
GTCACTGGGG AGGCGCACAA CGGCTGGATT GATGTAACCT GGGATGCTGG CGGCTCTAAC 
TCTTATCGTA TGGGCGCCGA AGGGAAGTTT GACCTCAAGC TTGCTCCAGG GTACGACCCT 
GAGCTGGCTG CCACAGCGCC ATCACCCAAA CCTGTCTCAT CCGCTGTTTC AGGTCCCGCC 
TCCTCCACGG TGGGACCCTC TGCGACGCCA GCAGCTGTTG GCGGAACCAC CATCACCACC 
TCATCCTCCT CCACCACCTC ATCGTCCTCG CAGCAGCCTT CCTGGAGCAG TTTGGTGAAA 
AATAACTGTC CCGACAAGGG AGGGGCCAGC TCCTCCAGCA GGAAGGGTAG CAGCAGCTCC 
GTCTGCAGCG TCGCTTCCTC CTCTGACATC AGTCTGAGCT CCTCCATTGG GCTGCCTGGC 
GCTGGGAGCC TGCAGCTGGA CAGGAAGGCC GAGGGGCTGC TGCTAGATCA GGCCGCGGGG 
GTGGAAGGGG TGACGGGGGG AGGGACCGGT TCCGATGGAC ATCAGCAAGA GCCGATAGTC 
GTTCTGTCCT CCGTGACCGA ATCCGGTTCG GCGTCCAGCT CCGGCACGCT CACCGCCGAC 
GTGTCAACGG CGGGTGACGA GCATCACTCC CCCTCTGCAA TGGCCACTGC TGTGGACCCG 
GCCACGGCCA TCTCCATGGG CTTGGTGAGC GTCAGCTCCC CGGACGTCAG CTCCGTGTCG 
GAGCCCTCCA GCAAAGACAC TCACTCCCAG AGGCCGCTGT GCTCGGCCGT GAACACCCGG 
CTGTCCGTCA GCTCCCTGCT GGCCGCCAGC GCTCCCATGA GCTCCAGCGC CAGCGTGCCC 
AATCTGTCCT CTCGGGAGGC CAGCCTCATG GAGTCCTTCG TCCGCCGCGC GCCCAACATG 
TCCCGCACCA ACGCTACCAA CAACATGAAC CTGAGCCGCA GCAGCAGCGA CAACAACACC 
AACACACTGG GCAGGAACGT GATGAGCACA GCCACATCTC CTCTTATGGG CGCTCAGAGC 
TTTCCTAACC TCACCACCAC TGGCACCACC TCCACCGTTA CCATGTCAAC CTCCATAGTA 
ACCTGCGGTA ATAATGTAGC CACAGCCACC ACGGGGCTGT CTGTGGGACA GTTGCTAAGC 
AACACACTGA CAACCAGCTT AACCTCCACA TCCAGCGAGA GCGACACGGG ACAGGAGGCC 
GAGTTCTCCC TCTATGACTT CCTGGACAGT TGCCGGGCCA ACACGCTGCT AGCAGAGCTG 
GACGATGAGG AGGACCTCCC AGAGCCCGAC GACGACGACG ATGAGAATGA AGACGACAAT 
CAGGAGGATC AGGAGTATGA GGAGGAGGAG GAGGAGTATG AGACGAAGGG AGGACGGAGG 
AGGACATGGG ACGATGACTT TGTCCTAAAA AGACAGTTTT CTGCTTTGGT CCCTGCCTTC 
GACCCCAGAC CAGGAAGAAC CAACGTCCAG CAGACCACCG ACCTGGAGAT CCCCCCACCA 
GGAACTCCGC GGTCGGAGGT TCGGGAGGAG GTGGAGTGTG CCCCATCCCC CCACCTCGCC 
CTCACACTGA AGGTGGCGGG TCTCGGCACG GCTCGGGAGG TGGAGATCCC GCTCTCCAAC 
TACAAGTTGA CCATCTTCTA CTATGTGCAG CGGCTCCTGC AGCTCTCCTG CAACGGCGCC 
GTAAAGACGG ACAAGCTGCG GCGCATCTGG GAGCCGACGT ACACGATCAT GTACAGAGAG 
CTCAAAGACT CGGACAAAGA GAAGGAGAGC GGGAAGATGG ACCTCTTTGA GCACGTTGTT 
GGAAGTCGTT CTGGAGGTCT GAGCCCCAGC TCGGGTTCAG CCAATCAGAG CAGCGAGATC 
CTGGGCTGGG CGAAGGAGGC GGTCCTGGCC AAGGCGGGCT GCAGCCAGAA CGCCTGCGGG 
GTGGAAGACG TCCTGCAGCT GCTGCGCATT CTCTATGTGA TTGGGAGAGA CTCTACTGCA 
GGCGGACGCA CGCTGCAAGA GGATGTTGAC GAGCTGCAGT TCAACTCGCC TCCTGAGGAG 
TTCAGCAGCA AGAAGATCAC CACCAAGATC CTGCAGCAGA TCGAGGAGCC CTTGGCTCTG 
GCTAGCGGAG CGCTGCCGGA CTGGTGTGAG CAGCTCACCT CCAAGTGTCC TTTCCTGATC 
CCCTTTGAGA CCAGACAGCT GTACTTCACC TGCACTGCCT TTGGGGCCTC CAGGGCGATC 
GTGTGGCTCC AGAACCGCCG CGAGGCGACC ATGGAGCGCT CACGGCCATC CACCACCGTC 
CGGCGAGACG ACCCCGGAGA GTTCAGAGTC GGTCGGCTTA AACACGAGCG CGTTAAAGTT 
CCTCGAGGGG AGTCCATGAT GGAGTGGGCG GAGTCTGTGA TGCAGATCCA CGCAAACAGG 
AAGTCTGTTT TAGAGGTGGA GTTTCTGGGT GAGGAGGGGA CGGGTCTGGG TCCGACTCTG 
GAGTTCTACG CTCTGGTGGC GGCGGAGTTT CAGAGAACGT CTCTGGGGAT CTGGCTCTGT 
GACGACGACT TCCCGGACGA CGAGTCCCGA CAGGTGGATC TGGGTGGAGG TCTGAAGCCT 
CCTGGCTTCT ACGTGCAGCG GTCCTGCGGG CTCTTCCCGG CGCCGTTCCC TCAGGACAGC 
GAGGAGCTGG AGCGGATTAC GCGGCTCTTC CACTTCCTGG GGATCTTCCT GGCCAAGTGC 
ATCCAGGACA ACCGGCTGGT GGACCTGCCC GTGTCCCAGC CTTTCTTCAA GCTGCTCTGC 
ATGGGGGACA TCAAGTCCAA CATGAGCAAG CTGCTGTACC AGTCCCGCGG CTCCCCCCAG 
GGCCACCTCT CCGAGAGGGC CCCCCTGCTG CTGCTGACGG AGGTGCAGTC GGAGGCGTCC 
ACAGAGGAGA GCCAGGAGAC CTACTCTGTG GGGAGCTTCG ACGAGGACTC CAAGTCAGAC 
TTCATCATGG ACCCCCCCAA ACCCAAGCCG CCCGCCTGGT TCCACGGCAT CATGTCCTGG 
GACGACTTCC ATCTGGTCAA CCCGCACAGA GCCAGTTTCC TGAAGGAGGT GAAGGAGCTG 
GCAGTGAGGA GGAGACAGAT TCTGACCAGC AAGAGTTTGT CAGAGGATGA GAAGAACACC 
AGGCTGCAGG ACCTGATGCT GAGGAACCCG CTGGGCTCTG GGCCCCCCTT AAGCGTCGAA 
GACCTCGGGT TGAACTTCCA GTTCTGTCCG TCCTCCAAGA TTCACGGCTT CTTCGCGGTG 
GATCTGAAAC CAAACGGGGA TGACGAGATG GTGAGTCTGG AGAACGCCGA GGAGTACGTG 
GAGCTGATGT TCGACTTCTG CATGCACGCC GGCATCCAGA AGCAGATGGA GGCGTTCAGA 
GAGGGTTTCA ACCGCGTCTT CCCCATGGAG AAGCTCAGCT CGTTCAGCCA GAAGGAGGTG 
CAGATGATCC TCTGCGGAAA CCAATCCCCC TGCTGGACCG CCGATGACAT CATCAGCTAC 
ACCGAGCCAA AGCTGGGATA CACCAGAGAC AGCCCTGGGT TCCTGCGCTT CATCCGAGTT 
TTATGCGGAA TGTCGACGGA CGAGAGGAAA GCTTTCCTGC AGTTCACCAC CGGCTGCTCC 
ACGCTGCCCC CCGGTGGCCT GGCTAACCTG CACCCGCGCC TCACCATCGT CAGAAAGGTG 
GACGCCACGG ACTCCAGCTA CCCATCGGTG AACACGTGCG TTCACTACCT GAAGCTGCCG 
GAGTACTCGT CAGAGGAGAT CATGAGGGAG CGGCTGTTGG CGGCCACCAT GGAGAAGGGC 
TTCCACCTCA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011488439.1
CDS	360..8054
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEASGLNCVLSFIRDSGHLVHKDTLHSAMAVVSRLCSKMEPQDSSLETCVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGSVSGSSSACKPGRPSTGATPSASDSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSALPNSMESALGGDERCVLDTMRLVDLLLVLLFEGRKALPKSTAGSAGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKRKKDASKEEEEGSEPKGDPEMAPFYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMVHYSSEVLLREVCDSETGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDVFLDQLARLGVINKVSTLAGPTSDDENEDEVKPEKEEEVQEDAKEIQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARSQVKPATASQPILSMVGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVKSMARELYDDHFKAVESMPRGVVVTLRNIATQLESAWELHINRQCLEGENTWRDLMKTALENLIVVLKDENTISPYEMCSSGLVQALFTVLSNSVELDLKHDCKPLMERINVFKAAFSENEDNESRPAVALVRKLIAVLESIERLPLHLYDTPGSSYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFERSSFVFVRKLREGQIFTFRHQHDFDENGIIYWVGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDSSALNCHTNDDKNAWFAVDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWSTLYNHIDDGSLNEPGSTATWPLDPSKEEKQGWRHIRIKQMGKNASGQTHYLSLSGLELYGTVTAVCEDQLGKAVKEAEANLRRQRRLFRSQVMKYIVPGARVIRGIDWKWRDQDGNPAGEGTVTGEAHNGWIDVTWDAGGSNSYRMGAEGKFDLKLAPGYDPELAATAPSPKPVSSAVSGPASSTVGPSATPAAVGGTTITTSSSSTTSSSSQQPSWSSLVKNNCPDKGGASSSSRKGSSSSVCSVASSSDISLSSSIGLPGAGSLQLDRKAEGLLLDQAAGVEGVTGGGTGSDGHQQEPIVVLSSVTESGSASSSGTLTADVSTAGDEHHSPSAMATAVDPATAISMGLVSVSSPDVSSVSEPSSKDTHSQRPLCSAVNTRLSVSSLLAASAPMSSSASVPNLSSREASLMESFVRRAPNMSRTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTTGTTSTVTMSTSIVTCGNNVATATTGLSVGQLLSNTLTTSLTSTSSESDTGQEAEFSLYDFLDSCRANTLLAELDDEEDLPEPDDDDDENEDDNQEDQEYEEEEEEYETKGGRRRTWDDDFVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPRSEVREEVECAPSPHLALTLKVAGLGTAREVEIPLSNYKLTIFYYVQRLLQLSCNGAVKTDKLRRIWEPTYTIMYRELKDSDKEKESGKMDLFEHVVGSRSGGLSPSSGSANQSSEILGWAKEAVLAKAGCSQNACGVEDVLQLLRILYVIGRDSTAGGRTLQEDVDELQFNSPPEEFSSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATMERSRPSTTVRRDDPGEFRVGRLKHERVKVPRGESMMEWAESVMQIHANRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTSLGIWLCDDDFPDDESRQVDLGGGLKPPGFYVQRSCGLFPAPFPQDSEELERITRLFHFLGIFLAKCIQDNRLVDLPVSQPFFKLLCMGDIKSNMSKLLYQSRGSPQGHLSERAPLLLLTEVQSEASTEESQETYSVGSFDEDSKSDFIMDPPKPKPPAWFHGIMSWDDFHLVNPHRASFLKEVKELAVRRRQILTSKSLSEDEKNTRLQDLMLRNPLGSGPPLSVEDLGLNFQFCPSSKIHGFFAVDLKPNGDDEMVSLENAEEYVELMFDFCMHAGIQKQMEAFREGFNRVFPMEKLSSFSQKEVQMILCGNQSPCWTADDIISYTEPKLGYTRDSPGFLRFIRVLCGMSTDERKAFLQFTTGCSTLPPGGLANLHPRLTIVRKVDATDSSYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_011490137.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes HECT domain E3 ubiquitin protein ligase 1 (hectd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011490137.2

ORF Insert Sequence:

ATGGCGGATG TGGACCCAGA CACGTTGCTG GAGTGGCTGC AGATGGGACA GGGCGATGAG 
CGGGACATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGCTGCTCAT GTCGGACAAC 
GTGGACCGCT GCTTTGAGAC ATGTCCTCCT CGAACGTTCC TCCCAGCGCT TTGTAAGATC 
TTCTTGGACG AGAGCGCTCC TGATAACGTG CTGGAGGTCA CGGCCAGAGC CATCACCTAC 
TACCTGGACG TGTCGGCAGA ATGCACGCGG AGGATCGTGG GTGTGGACGG GGCCATAAAG 
GCCCTGTGCA ACCGGCTGGT GGTGGTGGAA CTCAACAACA GGACCAGCAG GGATCTGGCT 
GAGCAGTGTG TTAAGGTGCT GGAGCTGATC TGTACCAGGG AATCTGGCGC CGTGTTTGAA 
GCCAGCGGTC TGAACTGCGT TTTGAGCTTC ATACGCGACA GTGGTCACCT GGTCCACAAA 
GACACTCTGC ACTCTGCAAT GGCAGTGGTG TCTCGCCTCT GTAGCAAGAT GGAGCCGCAG 
GACTCTTCTC TAGAAACCTG CGTGGAGTCT CTGTCCAGCC TCCTGAAACA TGAGGATCAC 
CAGGTGTCAG ACGGTGCTCT GCGTTGCTTC GCCTCATTGG CTGATCGGTT TACTCGTCGA 
GGCGTAGACC CCGCCCCTCT GGCCAAACAT GGACTGACGG AGGAGCTGCT TTCCCGCATG 
GCGGCTGCCG GAGGTTCCGT GTCGGGCTCT TCTTCTGCCT GTAAACCAGG TCGGCCCTCC 
ACTGGTGCCA CACCGTCTGC CTCCGACTCC AAACTGAGCA ACCAGGTGTC CACCATCGTT 
AGCCTGCTGT CCACGCTCTG CCGGGGGTCG CCAGTGGTCA CTCATGACTT GTTACGTTCG 
GCACTCCCTA ACTCCATGGA GTCTGCGCTG GGAGGAGACG AGCGCTGTGT GCTGGACACC 
ATGCGGCTGG TCGACCTGCT GCTGGTGCTT CTGTTCGAGG GGCGGAAAGC GCTGCCAAAG 
TCCACCGCCG GCTCAGCAGG TCGGATCCCG GGGCTTCGCC GGCTGGACAG CTCAGGGGAG 
CGGTCGCACC GACAGCTCAT CGACTGCATC CGCAGCAAAG ACACGGACGC CCTGATAGAT 
GCCATCGACA CTGGAGCTTT TGAAGTGAAC TTCATGGACG ACGTGGGACA GACGCTGCTG 
AACTGGGCCT CAGCTTTTGG AACACAGGAA ATGGTGGAGT TTTTGTGCGA GAGGGGTGCA 
GATGTGAACC GAGGCCAGAG GTCATCCTCG CTGCACTACG CTGCCTGTTT CGGACGGCCA 
CAAGTTGCCA AGACGCTGCT GCGGCACGGA GCCAACCCGG ACCTGAGGGA TGAGGACGGG 
AAGACTCCTT TGGACAAAGC CAGGGAGAGG GGACACAGTG AGGTGGTGGC CATCCTTCAG 
TCTCCGGGAG ACTGGATGTG TCCAGTGAAC AAAGGAGACG ACAAGAGGAA GAAAGATGCG 
AGTAAGGAAG AAGAGGAGGG CAGTGAACCC AAAGGAGACC CAGAAATGGC TCCTTTCTAC 
CTGAAAAGAC TCCTCCCTGT TTTTGCACAA ACCTTTCAGC AAACCATGCT GCCTTCCATC 
AGGAAAGCCA GTCTGGCCCT CATCAGGAAG ATGGTTCACT ACAGCAGCGA GGTTCTGCTG 
AGGGAGGTGT GCGACAGTGA GACGGGACAC AACCTGCCCA CAGTGCTGGT GGAGATCACC 
GCCACCGTCC TCGACCAGGA GGATGATGAC GACGGTCACC TGCTGGCTCT GCAGATCATC 
AGGGATCTGG TGGACAAAGG TGGAGATGTT TTCCTTGACC AGCTGGCCCG ACTCGGGGTC 
ATCAACAAGG TGTCGACCTT GGCCGGACCG ACGTCTGATG ATGAGAACGA GGATGAAGTC 
AAACCTGAAA AGGAGGAGGA GGTGCAGGAA GATGCAAAGG AAATCCAGCA GGGGAAGCCG 
TACCACTGGA GGGACTGGTC CATCATCAGA GGCAGGGACT GCCTCTACAT TTGGTCAGAC 
GCCGCCGCCC TCGAGCTCTC CAACGGCTCC AATGGCTGGT TTCGCTTCAT CTTGGATGGG 
AAGCTCGCCA CCATGTACTC GAGTGGGAGC CCAGAGGGGG GGTCAGACAG CTCAGAGTCT 
CGCAGTGAGT TTTTGGAGAA GCTGCAGCGT GCCAGAAGTC AGGTGAAACC AGCAACAGCC 
AGCCAGCCCA TCCTCTCCAT GGTCGGACCC ACCAAGCTGA CGGTGGGGAA CTGGTCTCTG 
ACCTGCCTGA AGGAAGGCGA GATTGCAATT CACAACTCAG ACGGGCAGCA GGCCACGATC 
CTGAAGGAGG ATCTGCCCGG CTTCGTGTTC GAGTCCAACA GAGGAACCAA ACACTCGTTC 
ACTGCTGAAA CGTCGCTCGG TTCTGAGTTT GTCACTGGCT GGACGGGGAA ACGTGGCAGA 
AAGCTGAAGT CCAAGCTGGA GAAGACCAAG CAGAAGGTGA AGAGCATGGC CAGGGAGCTG 
TACGACGACC ACTTCAAAGC TGTGGAGAGC ATGCCCAGAG GAGTGGTGGT CACCCTGAGG 
AACATCGCCA CGCAGCTGGA GTCCGCCTGG GAGCTGCACA TAAACCGACA GTGTCTGGAA 
GGAGAGAACA CGTGGAGGGA CCTGATGAAG ACCGCTCTGG AGAACCTGAT TGTGGTTTTG 
AAGGATGAAA ACACGATTTC TCCGTATGAA ATGTGCAGCA GCGGCCTGGT TCAGGCTCTG 
TTTACCGTCC TCAGCAACAG TGTGGAGCTG GACCTGAAAC ATGACTGTAA GCCTCTAATG 
GAAAGGATCA ACGTCTTTAA AGCTGCTTTC AGCGAGAATG AAGACAATGA AAGCCGACCA 
GCCGTTGCCT TAGTGCGGAA GCTGATAGCC GTCCTGGAAT CGATCGAGCG TCTGCCCCTG 
CACCTGTATG ACACGCCTGG ATCCTCCTAC AATCTGCAGA TTCTGACGAG GAGGCTGCGG 
TTCCGTCTGG AGCGAGCGCC TGGGGAGACC GCTCTCATTG ACCGGACGGG ACGCATGCTG 
AAGATGGAGC CGCTCGCCAC CGTAGAGTCT CTGGAGCAGT ACCTGCTGAA AATGGTCGCT 
AAACAGTGGT ACGACTTTGA ACGCTCATCC TTCGTTTTTG TGAGAAAACT GAGAGAAGGA 
CAGATCTTCA CCTTCAGGCA CCAGCACGAC TTTGACGAGA ACGGCATCAT CTACTGGGTC 
GGAACCAACG CCAAGACGGC GTACGAGTGG GTGAACCCCG CAGCGTACGG CCTGGTGGTG 
GTGACCTCCT CCGAGGGGAG GAATCTGCCT TATGGGCGTT TGGAGGACAT CCTGAGTCGG 
GATAGCTCCG CCCTCAACTG CCACACGAAC GACGACAAGA ACGCCTGGTT CGCAGTTGAC 
CTCGGCCTCT GGGTGATTCC GTCAGCGTAC ACTCTGAGGC ACGCCAGGGG GTACGGACGC 
TCTGCGCTCA GGAACTGGGT TTTCCAAGTG TCCAAGGACG GACAGAACTG GTCCACACTC 
TACAACCACA TCGACGATGG CAGTCTCAAT GAACCGGGGT CTACGGCCAC GTGGCCTCTG 
GACCCGTCCA AAGAGGAGAA GCAGGGCTGG AGACACATCC GCATCAAGCA GATGGGGAAG 
AACGCCAGCG GTCAGACACA CTACCTGTCC TTATCTGGGC TGGAGCTGTA CGGCACGGTC 
ACCGCCGTAT GCGAGGACCA GCTGGGTAAA GCTGTGAAAG AGGCGGAGGC CAACCTTCGT 
CGCCAGCGGC GCCTCTTCCG TTCTCAGGTC ATGAAGTACA TCGTCCCAGG TGCGCGGGTC 
ATCCGTGGCA TCGACTGGAA GTGGAGGGAC CAGGATGGAA ACCCGGCAGG GGAAGGCACT 
GTCACTGGGG AGGCGCACAA CGGCTGGATT GATGTAACCT GGGATGCTGG CGGCTCTAAC 
TCTTATCGTA TGGGCGCCGA AGGGAAGTTT GACCTCAAGC TTGCTCCAGG GTACGACCCT 
GAGCTGGCTG CCACAGCGCC ATCACCCAAA CCTGTCTCAT CCGCTGTTTC AGGTCCCGCC 
TCCTCCACGG TGGGACCCTC TGCGACGCCA GCAGCTGTTG GCGGAACCAC CATCACCACC 
TCATCCTCCT CCACCACCTC ATCGTCCTCG CAGCAGCCTT CCTGGAGCAG TTTGGTGAAA 
AATAACTGTC CCGACAAGGG AGGGGCCAGC TCCTCCAGCA GGAAGGGTAG CAGCAGCTCC 
GTCTGCAGCG TCGCTTCCTC CTCTGACATC AGTCTGAGCT CCTCCATTGG GCTGCCTGGC 
GCTGGGAGCC TGCAGCTGGA CAGGAAGGCC GAGGGGCTGC TGCTAGATCA GGCCGCGGGG 
GTGGAAGGGG TGACGGGGGG AGGGACCGGT TCCGATGGAC ATCAGCAAGA GCCGATAGTC 
GTTCTGTCCT CCGTGACCGA ATCCGGTTCG GCGTCCAGCT CCGGCACGCT CACCGCCGAC 
GTGTCAACGG CGGGTGACGA GCATCACTCC CCCTCTGCAA TGGCCACTGC TGTGGACCCG 
GCCACGGCCA TCTCCATGGG CTTGGTGAGC GTCAGCTCCC CGGACGTCAG CTCCGTGTCG 
GAGCCCTCCA GCAAAGACAC TCACTCCCAG AGGCCGCTGT GCTCGGCCGT GAACACCCGG 
CTGTCCGTCA GCTCCCTGCT GGCCGCCAGC GCTCCCATGA GCTCCAGCGC CAGCGTGCCC 
AATCTGTCCT CTCGGGAGGC CAGCCTCATG GAGTCCTTCG TCCGCCGCGC GCCCAACATG 
TCCCGCACCA ACGCTACCAA CAACATGAAC CTGAGCCGCA GCAGCAGCGA CAACAACACC 
AACACACTGG GCAGGAACGT GATGAGCACA GCCACATCTC CTCTTATGGG CGCTCAGAGC 
TTTCCTAACC TCACCACCAC TGGCACCACC TCCACCGTTA CCATGTCAAC CTCCATAGTA 
ACCTGCGGTA ATAATGTAGC CACAGCCACC ACGGGGCTGT CTGTGGGACA GTTGCTAAGC 
AACACACTGA CAACCAGCTT AACCTCCACA TCCAGCGAGA GCGACACGGG ACAGGAGGCC 
GAGTTCTCCC TCTATGACTT CCTGGACAGT TGCCGGGCCA ACACGCTGCT AGCAGAGCTG 
GACGATGAGG AGGACCTCCC AGAGCCCGAC GACGACGACG ATGAGAATGA AGACGACAAT 
CAGGAGGATC AGGAGTATGA GGAGGAGGAG GAGGAGTATG AGACGAAGGG AGGACGGAGG 
AGGACATGGG ACGATGACTT TGTCCTAAAA AGACAGTTTT CTGCTTTGGT CCCTGCCTTC 
GACCCCAGAC CAGGAAGAAC CAACGTCCAG CAGACCACCG ACCTGGAGAT CCCCCCACCA 
GGAACTCCGC GGTCGGAGGT TCGGGAGGAG GTGGAGTGTG CCCCATCCCC CCACCTCGCC 
CTCACACTGA AGGTGGCGGG TCTCGGCACG GCTCGGGAGG TGGAGATCCC GCTCTCCAAC 
TACAAGTTGA CCATCTTCTA CTATGTGCAG CGGCTCCTGC AGCTCTCCTG CAACGGCGCC 
GTAAAGACGG ACAAGCTGCG GCGCATCTGG GAGCCGACGT ACACGATCAT GTACAGAGAG 
CTCAAAGACT CGGACAAAGA GAAGGAGAGC GGGAAGATGG ACCTCTTTGA GCACGTTGTT 
GGAAGTCGTT CTGGAGGTCT GAGCCCCAGC TCGGGTTCAG CCAATCAGAG CAGCGAGATC 
CTGGGCTGGG CGAAGGAGGC GGTCCTGGCC AAGGCGGGCT GCAGCCAGAA CGCCTGCGGG 
GTGGAAGACG TCCTGCAGCT GCTGCGCATT CTCTATGTGA TTGGGAGAGA CTCTACTGCA 
GGCGGACGCA CGCTGCAAGA GGATGTTGAC GAGCTGCAGT TCAACTCGCC TCCTGAGGAG 
TTCAGCAGCA AGAAGATCAC CACCAAGATC CTGCAGCAGA TCGAGGAGCC CTTGGCTCTG 
GCTAGCGGAG CGCTGCCGGA CTGGTGTGAG CAGCTCACCT CCAAGTGTCC TTTCCTGATC 
CCCTTTGAGA CCAGACAGCT GTACTTCACC TGCACTGCCT TTGGGGCCTC CAGGGCGATC 
GTGTGGCTCC AGAACCGCCG CGAGGCGACC ATGGAGCGCT CACGGCCATC CACCACCGTC 
CGGCGAGACG ACCCCGGAGA GTTCAGAGTC GGTCGGCTTA AACACGAGCG CGTTAAAGTT 
CCTCGAGGGG AGTCCATGAT GGAGTGGGCG GAGTCTGTGA TGCAGATCCA CGCAAACAGG 
AAGTCTGTTT TAGAGGTGGA GTTTCTGGGT GAGGAGGGGA CGGGTCTGGG TCCGACTCTG 
GAGTTCTACG CTCTGGTGGC GGCGGAGTTT CAGAGAACGT CTCTGGGGAT CTGGCTCTGT 
GACGACGACT TCCCGGACGA CGAGTCCCGA CAGGTGGATC TGGGTGGAGG TCTGAAGCCT 
CCTGGCTTCT ACGTGCAGCG GTCCTGCGGG CTCTTCCCGG CGCCGTTCCC TCAGGACAGC 
GAGGAGCTGG AGCGGATTAC GCGGCTCTTC CACTTCCTGG GGATCTTCCT GGCCAAGTGC 
ATCCAGGACA ACCGGCTGGT GGACCTGCCC GTGTCCCAGC CTTTCTTCAA GCTGCTCTGC 
ATGGGGGACA TCAAGTCCAA CATGAGCAAG CTGCTGTACC AGTCCCGCGG CTCCCCCCAG 
GGCCACCTCT CCGAGAGGGC CCCCCTGCTG CTGCTGACGG AGGTGCAGTC GGAGGCGTCC 
ACAGAGGAGA GCCAGGAGAC CTACTCTGTG GGGAGCTTCG ACGAGGACTC CAAGTCAGAC 
TTCATCATGG ACCCCCCCAA ACCCAAGCCG CCCGCCTGGT TCCACGGCAT CATGTCCTGG 
GACGACTTCC ATCTGGTCAA CCCGCACAGA GCCAGTTTCC TGAAGGAGGT GAAGGAGCTG 
GCAGTGAGGA GGAGACAGAT TCTGACCAGC AAGAGTTTGT CAGAGGATGA GAAGAACACC 
AGGCTGCAGG ACCTGATGCT GAGGAACCCG CTGGGCTCTG GGCCCCCCTT AAGCGTCGAA 
GACCTCGGGT TGAACTTCCA GTTCTGTCCG TCCTCCAAGA TTCACGGCTT CTTCGCGGTG 
GATCTGAAAC CAAACGGGGA TGACGAGATG GTGAGTCTGG AGAACGCCGA GGAGTACGTG 
GAGCTGATGT TCGACTTCTG CATGCACGCC GGCATCCAGA AGCAGATGGA GGCGTTCAGA 
GAGGGTTTCA ACCGCGTCTT CCCCATGGAG AAGCTCAGCT CGTTCAGCCA GAAGGAGGTG 
CAGATGATCC TCTGCGGAAA CCAATCCCCC TGCTGGACCG CCGATGACAT CATCAGCTAC 
ACCGAGCCAA AGCTGGGATA CACCAGAGAC AGCCCTGGGT TCCTGCGCTT CATCCGAGTT 
TTATGCGGAA TGTCGACGGA CGAGAGGAAA GCTTTCCTGC AGTTCACCAC CGGCTGCTCC 
ACGCTGCCCC CCGGTGGCCT GGCTAACCTG CACCCGCGCC TCACCATCGT CAGAAAGGTG 
GACGCCACGG ACTCCAGCTA CCCATCGGTG AACACGTGCG TTCACTACCT GAAGCTGCCG 
GAGTACTCGT CAGAGGAGAT CATGAGGGAG CGGCTGTTGG CGGCCACCAT GGAGAAGGGC 
TTCCACCTCA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hectd1 ( XM_011490137.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011488439.1 Oryzias latipes HECT domain E3 ubiquitin protein ligase 1 (hectd1), transcript variant X2, mRNA.

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CloneID	OJa31518
Clone ID Related Accession (Same CDS sequence)	XM_011490137.2 , XM_011490137.3
Accession Version	XM_011490137.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_011490137.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG TGGACCCAGA CACGTTGCTG GAGTGGCTGC AGATGGGACA GGGCGATGAG 
CGGGACATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGCTGCTCAT GTCGGACAAC 
GTGGACCGCT GCTTTGAGAC ATGTCCTCCT CGAACGTTCC TCCCAGCGCT TTGTAAGATC 
TTCTTGGACG AGAGCGCTCC TGATAACGTG CTGGAGGTCA CGGCCAGAGC CATCACCTAC 
TACCTGGACG TGTCGGCAGA ATGCACGCGG AGGATCGTGG GTGTGGACGG GGCCATAAAG 
GCCCTGTGCA ACCGGCTGGT GGTGGTGGAA CTCAACAACA GGACCAGCAG GGATCTGGCT 
GAGCAGTGTG TTAAGGTGCT GGAGCTGATC TGTACCAGGG AATCTGGCGC CGTGTTTGAA 
GCCAGCGGTC TGAACTGCGT TTTGAGCTTC ATACGCGACA GTGGTCACCT GGTCCACAAA 
GACACTCTGC ACTCTGCAAT GGCAGTGGTG TCTCGCCTCT GTAGCAAGAT GGAGCCGCAG 
GACTCTTCTC TAGAAACCTG CGTGGAGTCT CTGTCCAGCC TCCTGAAACA TGAGGATCAC 
CAGGTGTCAG ACGGTGCTCT GCGTTGCTTC GCCTCATTGG CTGATCGGTT TACTCGTCGA 
GGCGTAGACC CCGCCCCTCT GGCCAAACAT GGACTGACGG AGGAGCTGCT TTCCCGCATG 
GCGGCTGCCG GAGGTTCCGT GTCGGGCTCT TCTTCTGCCT GTAAACCAGG TCGGCCCTCC 
ACTGGTGCCA CACCGTCTGC CTCCGACTCC AAACTGAGCA ACCAGGTGTC CACCATCGTT 
AGCCTGCTGT CCACGCTCTG CCGGGGGTCG CCAGTGGTCA CTCATGACTT GTTACGTTCG 
GCACTCCCTA ACTCCATGGA GTCTGCGCTG GGAGGAGACG AGCGCTGTGT GCTGGACACC 
ATGCGGCTGG TCGACCTGCT GCTGGTGCTT CTGTTCGAGG GGCGGAAAGC GCTGCCAAAG 
TCCACCGCCG GCTCAGCAGG TCGGATCCCG GGGCTTCGCC GGCTGGACAG CTCAGGGGAG 
CGGTCGCACC GACAGCTCAT CGACTGCATC CGCAGCAAAG ACACGGACGC CCTGATAGAT 
GCCATCGACA CTGGAGCTTT TGAAGTGAAC TTCATGGACG ACGTGGGACA GACGCTGCTG 
AACTGGGCCT CAGCTTTTGG AACACAGGAA ATGGTGGAGT TTTTGTGCGA GAGGGGTGCA 
GATGTGAACC GAGGCCAGAG GTCATCCTCG CTGCACTACG CTGCCTGTTT CGGACGGCCA 
CAAGTTGCCA AGACGCTGCT GCGGCACGGA GCCAACCCGG ACCTGAGGGA TGAGGACGGG 
AAGACTCCTT TGGACAAAGC CAGGGAGAGG GGACACAGTG AGGTGGTGGC CATCCTTCAG 
TCTCCGGGAG ACTGGATGTG TCCAGTGAAC AAAGGAGACG ACAAGAGGAA GAAAGATGCG 
AGTAAGGAAG AAGAGGAGGG CAGTGAACCC AAAGGAGACC CAGAAATGGC TCCTTTCTAC 
CTGAAAAGAC TCCTCCCTGT TTTTGCACAA ACCTTTCAGC AAACCATGCT GCCTTCCATC 
AGGAAAGCCA GTCTGGCCCT CATCAGGAAG ATGGTTCACT ACAGCAGCGA GGTTCTGCTG 
AGGGAGGTGT GCGACAGTGA GACGGGACAC AACCTGCCCA CAGTGCTGGT GGAGATCACC 
GCCACCGTCC TCGACCAGGA GGATGATGAC GACGGTCACC TGCTGGCTCT GCAGATCATC 
AGGGATCTGG TGGACAAAGG TGGAGATGTT TTCCTTGACC AGCTGGCCCG ACTCGGGGTC 
ATCAACAAGG TGTCGACCTT GGCCGGACCG ACGTCTGATG ATGAGAACGA GGATGAAGTC 
AAACCTGAAA AGGAGGAGGA GGTGCAGGAA GATGCAAAGG AAATCCAGCA GGGGAAGCCG 
TACCACTGGA GGGACTGGTC CATCATCAGA GGCAGGGACT GCCTCTACAT TTGGTCAGAC 
GCCGCCGCCC TCGAGCTCTC CAACGGCTCC AATGGCTGGT TTCGCTTCAT CTTGGATGGG 
AAGCTCGCCA CCATGTACTC GAGTGGGAGC CCAGAGGGGG GGTCAGACAG CTCAGAGTCT 
CGCAGTGAGT TTTTGGAGAA GCTGCAGCGT GCCAGAAGTC AGGTGAAACC AGCAACAGCC 
AGCCAGCCCA TCCTCTCCAT GGTCGGACCC ACCAAGCTGA CGGTGGGGAA CTGGTCTCTG 
ACCTGCCTGA AGGAAGGCGA GATTGCAATT CACAACTCAG ACGGGCAGCA GGCCACGATC 
CTGAAGGAGG ATCTGCCCGG CTTCGTGTTC GAGTCCAACA GAGGAACCAA ACACTCGTTC 
ACTGCTGAAA CGTCGCTCGG TTCTGAGTTT GTCACTGGCT GGACGGGGAA ACGTGGCAGA 
AAGCTGAAGT CCAAGCTGGA GAAGACCAAG CAGAAGGTGA AGAGCATGGC CAGGGAGCTG 
TACGACGACC ACTTCAAAGC TGTGGAGAGC ATGCCCAGAG GAGTGGTGGT CACCCTGAGG 
AACATCGCCA CGCAGCTGGA GTCCGCCTGG GAGCTGCACA TAAACCGACA GTGTCTGGAA 
GGAGAGAACA CGTGGAGGGA CCTGATGAAG ACCGCTCTGG AGAACCTGAT TGTGGTTTTG 
AAGGATGAAA ACACGATTTC TCCGTATGAA ATGTGCAGCA GCGGCCTGGT TCAGGCTCTG 
TTTACCGTCC TCAGCAACAG TGTGGAGCTG GACCTGAAAC ATGACTGTAA GCCTCTAATG 
GAAAGGATCA ACGTCTTTAA AGCTGCTTTC AGCGAGAATG AAGACAATGA AAGCCGACCA 
GCCGTTGCCT TAGTGCGGAA GCTGATAGCC GTCCTGGAAT CGATCGAGCG TCTGCCCCTG 
CACCTGTATG ACACGCCTGG ATCCTCCTAC AATCTGCAGA TTCTGACGAG GAGGCTGCGG 
TTCCGTCTGG AGCGAGCGCC TGGGGAGACC GCTCTCATTG ACCGGACGGG ACGCATGCTG 
AAGATGGAGC CGCTCGCCAC CGTAGAGTCT CTGGAGCAGT ACCTGCTGAA AATGGTCGCT 
AAACAGTGGT ACGACTTTGA ACGCTCATCC TTCGTTTTTG TGAGAAAACT GAGAGAAGGA 
CAGATCTTCA CCTTCAGGCA CCAGCACGAC TTTGACGAGA ACGGCATCAT CTACTGGGTC 
GGAACCAACG CCAAGACGGC GTACGAGTGG GTGAACCCCG CAGCGTACGG CCTGGTGGTG 
GTGACCTCCT CCGAGGGGAG GAATCTGCCT TATGGGCGTT TGGAGGACAT CCTGAGTCGG 
GATAGCTCCG CCCTCAACTG CCACACGAAC GACGACAAGA ACGCCTGGTT CGCAGTTGAC 
CTCGGCCTCT GGGTGATTCC GTCAGCGTAC ACTCTGAGGC ACGCCAGGGG GTACGGACGC 
TCTGCGCTCA GGAACTGGGT TTTCCAAGTG TCCAAGGACG GACAGAACTG GTCCACACTC 
TACAACCACA TCGACGATGG CAGTCTCAAT GAACCGGGGT CTACGGCCAC GTGGCCTCTG 
GACCCGTCCA AAGAGGAGAA GCAGGGCTGG AGACACATCC GCATCAAGCA GATGGGGAAG 
AACGCCAGCG GTCAGACACA CTACCTGTCC TTATCTGGGC TGGAGCTGTA CGGCACGGTC 
ACCGCCGTAT GCGAGGACCA GCTGGGTAAA GCTGTGAAAG AGGCGGAGGC CAACCTTCGT 
CGCCAGCGGC GCCTCTTCCG TTCTCAGGTC ATGAAGTACA TCGTCCCAGG TGCGCGGGTC 
ATCCGTGGCA TCGACTGGAA GTGGAGGGAC CAGGATGGAA ACCCGGCAGG GGAAGGCACT 
GTCACTGGGG AGGCGCACAA CGGCTGGATT GATGTAACCT GGGATGCTGG CGGCTCTAAC 
TCTTATCGTA TGGGCGCCGA AGGGAAGTTT GACCTCAAGC TTGCTCCAGG GTACGACCCT 
GAGCTGGCTG CCACAGCGCC ATCACCCAAA CCTGTCTCAT CCGCTGTTTC AGGTCCCGCC 
TCCTCCACGG TGGGACCCTC TGCGACGCCA GCAGCTGTTG GCGGAACCAC CATCACCACC 
TCATCCTCCT CCACCACCTC ATCGTCCTCG CAGCAGCCTT CCTGGAGCAG TTTGGTGAAA 
AATAACTGTC CCGACAAGGG AGGGGCCAGC TCCTCCAGCA GGAAGGGTAG CAGCAGCTCC 
GTCTGCAGCG TCGCTTCCTC CTCTGACATC AGTCTGAGCT CCTCCATTGG GCTGCCTGGC 
GCTGGGAGCC TGCAGCTGGA CAGGAAGGCC GAGGGGCTGC TGCTAGATCA GGCCGCGGGG 
GTGGAAGGGG TGACGGGGGG AGGGACCGGT TCCGATGGAC ATCAGCAAGA GCCGATAGTC 
GTTCTGTCCT CCGTGACCGA ATCCGGTTCG GCGTCCAGCT CCGGCACGCT CACCGCCGAC 
GTGTCAACGG CGGGTGACGA GCATCACTCC CCCTCTGCAA TGGCCACTGC TGTGGACCCG 
GCCACGGCCA TCTCCATGGG CTTGGTGAGC GTCAGCTCCC CGGACGTCAG CTCCGTGTCG 
GAGCCCTCCA GCAAAGACAC TCACTCCCAG AGGCCGCTGT GCTCGGCCGT GAACACCCGG 
CTGTCCGTCA GCTCCCTGCT GGCCGCCAGC GCTCCCATGA GCTCCAGCGC CAGCGTGCCC 
AATCTGTCCT CTCGGGAGGC CAGCCTCATG GAGTCCTTCG TCCGCCGCGC GCCCAACATG 
TCCCGCACCA ACGCTACCAA CAACATGAAC CTGAGCCGCA GCAGCAGCGA CAACAACACC 
AACACACTGG GCAGGAACGT GATGAGCACA GCCACATCTC CTCTTATGGG CGCTCAGAGC 
TTTCCTAACC TCACCACCAC TGGCACCACC TCCACCGTTA CCATGTCAAC CTCCATAGTA 
ACCTGCGGTA ATAATGTAGC CACAGCCACC ACGGGGCTGT CTGTGGGACA GTTGCTAAGC 
AACACACTGA CAACCAGCTT AACCTCCACA TCCAGCGAGA GCGACACGGG ACAGGAGGCC 
GAGTTCTCCC TCTATGACTT CCTGGACAGT TGCCGGGCCA ACACGCTGCT AGCAGAGCTG 
GACGATGAGG AGGACCTCCC AGAGCCCGAC GACGACGACG ATGAGAATGA AGACGACAAT 
CAGGAGGATC AGGAGTATGA GGAGGAGGAG GAGGAGTATG AGACGAAGGG AGGACGGAGG 
AGGACATGGG ACGATGACTT TGTCCTAAAA AGACAGTTTT CTGCTTTGGT CCCTGCCTTC 
GACCCCAGAC CAGGAAGAAC CAACGTCCAG CAGACCACCG ACCTGGAGAT CCCCCCACCA 
GGAACTCCGC GGTCGGAGGT TCGGGAGGAG GTGGAGTGTG CCCCATCCCC CCACCTCGCC 
CTCACACTGA AGGTGGCGGG TCTCGGCACG GCTCGGGAGG TGGAGATCCC GCTCTCCAAC 
TACAAGTTGA CCATCTTCTA CTATGTGCAG CGGCTCCTGC AGCTCTCCTG CAACGGCGCC 
GTAAAGACGG ACAAGCTGCG GCGCATCTGG GAGCCGACGT ACACGATCAT GTACAGAGAG 
CTCAAAGACT CGGACAAAGA GAAGGAGAGC GGGAAGATGG ACCTCTTTGA GCACGTTGTT 
GGAAGTCGTT CTGGAGGTCT GAGCCCCAGC TCGGGTTCAG CCAATCAGAG CAGCGAGATC 
CTGGGCTGGG CGAAGGAGGC GGTCCTGGCC AAGGCGGGCT GCAGCCAGAA CGCCTGCGGG 
GTGGAAGACG TCCTGCAGCT GCTGCGCATT CTCTATGTGA TTGGGAGAGA CTCTACTGCA 
GGCGGACGCA CGCTGCAAGA GGATGTTGAC GAGCTGCAGT TCAACTCGCC TCCTGAGGAG 
TTCAGCAGCA AGAAGATCAC CACCAAGATC CTGCAGCAGA TCGAGGAGCC CTTGGCTCTG 
GCTAGCGGAG CGCTGCCGGA CTGGTGTGAG CAGCTCACCT CCAAGTGTCC TTTCCTGATC 
CCCTTTGAGA CCAGACAGCT GTACTTCACC TGCACTGCCT TTGGGGCCTC CAGGGCGATC 
GTGTGGCTCC AGAACCGCCG CGAGGCGACC ATGGAGCGCT CACGGCCATC CACCACCGTC 
CGGCGAGACG ACCCCGGAGA GTTCAGAGTC GGTCGGCTTA AACACGAGCG CGTTAAAGTT 
CCTCGAGGGG AGTCCATGAT GGAGTGGGCG GAGTCTGTGA TGCAGATCCA CGCAAACAGG 
AAGTCTGTTT TAGAGGTGGA GTTTCTGGGT GAGGAGGGGA CGGGTCTGGG TCCGACTCTG 
GAGTTCTACG CTCTGGTGGC GGCGGAGTTT CAGAGAACGT CTCTGGGGAT CTGGCTCTGT 
GACGACGACT TCCCGGACGA CGAGTCCCGA CAGGTGGATC TGGGTGGAGG TCTGAAGCCT 
CCTGGCTTCT ACGTGCAGCG GTCCTGCGGG CTCTTCCCGG CGCCGTTCCC TCAGGACAGC 
GAGGAGCTGG AGCGGATTAC GCGGCTCTTC CACTTCCTGG GGATCTTCCT GGCCAAGTGC 
ATCCAGGACA ACCGGCTGGT GGACCTGCCC GTGTCCCAGC CTTTCTTCAA GCTGCTCTGC 
ATGGGGGACA TCAAGTCCAA CATGAGCAAG CTGCTGTACC AGTCCCGCGG CTCCCCCCAG 
GGCCACCTCT CCGAGAGGGC CCCCCTGCTG CTGCTGACGG AGGTGCAGTC GGAGGCGTCC 
ACAGAGGAGA GCCAGGAGAC CTACTCTGTG GGGAGCTTCG ACGAGGACTC CAAGTCAGAC 
TTCATCATGG ACCCCCCCAA ACCCAAGCCG CCCGCCTGGT TCCACGGCAT CATGTCCTGG 
GACGACTTCC ATCTGGTCAA CCCGCACAGA GCCAGTTTCC TGAAGGAGGT GAAGGAGCTG 
GCAGTGAGGA GGAGACAGAT TCTGACCAGC AAGAGTTTGT CAGAGGATGA GAAGAACACC 
AGGCTGCAGG ACCTGATGCT GAGGAACCCG CTGGGCTCTG GGCCCCCCTT AAGCGTCGAA 
GACCTCGGGT TGAACTTCCA GTTCTGTCCG TCCTCCAAGA TTCACGGCTT CTTCGCGGTG 
GATCTGAAAC CAAACGGGGA TGACGAGATG GTGAGTCTGG AGAACGCCGA GGAGTACGTG 
GAGCTGATGT TCGACTTCTG CATGCACGCC GGCATCCAGA AGCAGATGGA GGCGTTCAGA 
GAGGGTTTCA ACCGCGTCTT CCCCATGGAG AAGCTCAGCT CGTTCAGCCA GAAGGAGGTG 
CAGATGATCC TCTGCGGAAA CCAATCCCCC TGCTGGACCG CCGATGACAT CATCAGCTAC 
ACCGAGCCAA AGCTGGGATA CACCAGAGAC AGCCCTGGGT TCCTGCGCTT CATCCGAGTT 
TTATGCGGAA TGTCGACGGA CGAGAGGAAA GCTTTCCTGC AGTTCACCAC CGGCTGCTCC 
ACGCTGCCCC CCGGTGGCCT GGCTAACCTG CACCCGCGCC TCACCATCGT CAGAAAGGTG 
GACGCCACGG ACTCCAGCTA CCCATCGGTG AACACGTGCG TTCACTACCT GAAGCTGCCG 
GAGTACTCGT CAGAGGAGAT CATGAGGGAG CGGCTGTTGG CGGCCACCAT GGAGAAGGGC 
TTCCACCTCA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011488439.1
CDS	459..8153
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEASGLNCVLSFIRDSGHLVHKDTLHSAMAVVSRLCSKMEPQDSSLETCVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGSVSGSSSACKPGRPSTGATPSASDSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSALPNSMESALGGDERCVLDTMRLVDLLLVLLFEGRKALPKSTAGSAGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKRKKDASKEEEEGSEPKGDPEMAPFYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMVHYSSEVLLREVCDSETGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDVFLDQLARLGVINKVSTLAGPTSDDENEDEVKPEKEEEVQEDAKEIQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARSQVKPATASQPILSMVGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVKSMARELYDDHFKAVESMPRGVVVTLRNIATQLESAWELHINRQCLEGENTWRDLMKTALENLIVVLKDENTISPYEMCSSGLVQALFTVLSNSVELDLKHDCKPLMERINVFKAAFSENEDNESRPAVALVRKLIAVLESIERLPLHLYDTPGSSYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFERSSFVFVRKLREGQIFTFRHQHDFDENGIIYWVGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDSSALNCHTNDDKNAWFAVDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWSTLYNHIDDGSLNEPGSTATWPLDPSKEEKQGWRHIRIKQMGKNASGQTHYLSLSGLELYGTVTAVCEDQLGKAVKEAEANLRRQRRLFRSQVMKYIVPGARVIRGIDWKWRDQDGNPAGEGTVTGEAHNGWIDVTWDAGGSNSYRMGAEGKFDLKLAPGYDPELAATAPSPKPVSSAVSGPASSTVGPSATPAAVGGTTITTSSSSTTSSSSQQPSWSSLVKNNCPDKGGASSSSRKGSSSSVCSVASSSDISLSSSIGLPGAGSLQLDRKAEGLLLDQAAGVEGVTGGGTGSDGHQQEPIVVLSSVTESGSASSSGTLTADVSTAGDEHHSPSAMATAVDPATAISMGLVSVSSPDVSSVSEPSSKDTHSQRPLCSAVNTRLSVSSLLAASAPMSSSASVPNLSSREASLMESFVRRAPNMSRTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTTGTTSTVTMSTSIVTCGNNVATATTGLSVGQLLSNTLTTSLTSTSSESDTGQEAEFSLYDFLDSCRANTLLAELDDEEDLPEPDDDDDENEDDNQEDQEYEEEEEEYETKGGRRRTWDDDFVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPRSEVREEVECAPSPHLALTLKVAGLGTAREVEIPLSNYKLTIFYYVQRLLQLSCNGAVKTDKLRRIWEPTYTIMYRELKDSDKEKESGKMDLFEHVVGSRSGGLSPSSGSANQSSEILGWAKEAVLAKAGCSQNACGVEDVLQLLRILYVIGRDSTAGGRTLQEDVDELQFNSPPEEFSSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATMERSRPSTTVRRDDPGEFRVGRLKHERVKVPRGESMMEWAESVMQIHANRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTSLGIWLCDDDFPDDESRQVDLGGGLKPPGFYVQRSCGLFPAPFPQDSEELERITRLFHFLGIFLAKCIQDNRLVDLPVSQPFFKLLCMGDIKSNMSKLLYQSRGSPQGHLSERAPLLLLTEVQSEASTEESQETYSVGSFDEDSKSDFIMDPPKPKPPAWFHGIMSWDDFHLVNPHRASFLKEVKELAVRRRQILTSKSLSEDEKNTRLQDLMLRNPLGSGPPLSVEDLGLNFQFCPSSKIHGFFAVDLKPNGDDEMVSLENAEEYVELMFDFCMHAGIQKQMEAFREGFNRVFPMEKLSSFSQKEVQMILCGNQSPCWTADDIISYTEPKLGYTRDSPGFLRFIRVLCGMSTDERKAFLQFTTGCSTLPPGGLANLHPRLTIVRKVDATDSSYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_011490137.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes HECT domain E3 ubiquitin protein ligase 1 (hectd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011490137.3

ORF Insert Sequence:

ATGGCGGATG TGGACCCAGA CACGTTGCTG GAGTGGCTGC AGATGGGACA GGGCGATGAG 
CGGGACATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGCTGCTCAT GTCGGACAAC 
GTGGACCGCT GCTTTGAGAC ATGTCCTCCT CGAACGTTCC TCCCAGCGCT TTGTAAGATC 
TTCTTGGACG AGAGCGCTCC TGATAACGTG CTGGAGGTCA CGGCCAGAGC CATCACCTAC 
TACCTGGACG TGTCGGCAGA ATGCACGCGG AGGATCGTGG GTGTGGACGG GGCCATAAAG 
GCCCTGTGCA ACCGGCTGGT GGTGGTGGAA CTCAACAACA GGACCAGCAG GGATCTGGCT 
GAGCAGTGTG TTAAGGTGCT GGAGCTGATC TGTACCAGGG AATCTGGCGC CGTGTTTGAA 
GCCAGCGGTC TGAACTGCGT TTTGAGCTTC ATACGCGACA GTGGTCACCT GGTCCACAAA 
GACACTCTGC ACTCTGCAAT GGCAGTGGTG TCTCGCCTCT GTAGCAAGAT GGAGCCGCAG 
GACTCTTCTC TAGAAACCTG CGTGGAGTCT CTGTCCAGCC TCCTGAAACA TGAGGATCAC 
CAGGTGTCAG ACGGTGCTCT GCGTTGCTTC GCCTCATTGG CTGATCGGTT TACTCGTCGA 
GGCGTAGACC CCGCCCCTCT GGCCAAACAT GGACTGACGG AGGAGCTGCT TTCCCGCATG 
GCGGCTGCCG GAGGTTCCGT GTCGGGCTCT TCTTCTGCCT GTAAACCAGG TCGGCCCTCC 
ACTGGTGCCA CACCGTCTGC CTCCGACTCC AAACTGAGCA ACCAGGTGTC CACCATCGTT 
AGCCTGCTGT CCACGCTCTG CCGGGGGTCG CCAGTGGTCA CTCATGACTT GTTACGTTCG 
GCACTCCCTA ACTCCATGGA GTCTGCGCTG GGAGGAGACG AGCGCTGTGT GCTGGACACC 
ATGCGGCTGG TCGACCTGCT GCTGGTGCTT CTGTTCGAGG GGCGGAAAGC GCTGCCAAAG 
TCCACCGCCG GCTCAGCAGG TCGGATCCCG GGGCTTCGCC GGCTGGACAG CTCAGGGGAG 
CGGTCGCACC GACAGCTCAT CGACTGCATC CGCAGCAAAG ACACGGACGC CCTGATAGAT 
GCCATCGACA CTGGAGCTTT TGAAGTGAAC TTCATGGACG ACGTGGGACA GACGCTGCTG 
AACTGGGCCT CAGCTTTTGG AACACAGGAA ATGGTGGAGT TTTTGTGCGA GAGGGGTGCA 
GATGTGAACC GAGGCCAGAG GTCATCCTCG CTGCACTACG CTGCCTGTTT CGGACGGCCA 
CAAGTTGCCA AGACGCTGCT GCGGCACGGA GCCAACCCGG ACCTGAGGGA TGAGGACGGG 
AAGACTCCTT TGGACAAAGC CAGGGAGAGG GGACACAGTG AGGTGGTGGC CATCCTTCAG 
TCTCCGGGAG ACTGGATGTG TCCAGTGAAC AAAGGAGACG ACAAGAGGAA GAAAGATGCG 
AGTAAGGAAG AAGAGGAGGG CAGTGAACCC AAAGGAGACC CAGAAATGGC TCCTTTCTAC 
CTGAAAAGAC TCCTCCCTGT TTTTGCACAA ACCTTTCAGC AAACCATGCT GCCTTCCATC 
AGGAAAGCCA GTCTGGCCCT CATCAGGAAG ATGGTTCACT ACAGCAGCGA GGTTCTGCTG 
AGGGAGGTGT GCGACAGTGA GACGGGACAC AACCTGCCCA CAGTGCTGGT GGAGATCACC 
GCCACCGTCC TCGACCAGGA GGATGATGAC GACGGTCACC TGCTGGCTCT GCAGATCATC 
AGGGATCTGG TGGACAAAGG TGGAGATGTT TTCCTTGACC AGCTGGCCCG ACTCGGGGTC 
ATCAACAAGG TGTCGACCTT GGCCGGACCG ACGTCTGATG ATGAGAACGA GGATGAAGTC 
AAACCTGAAA AGGAGGAGGA GGTGCAGGAA GATGCAAAGG AAATCCAGCA GGGGAAGCCG 
TACCACTGGA GGGACTGGTC CATCATCAGA GGCAGGGACT GCCTCTACAT TTGGTCAGAC 
GCCGCCGCCC TCGAGCTCTC CAACGGCTCC AATGGCTGGT TTCGCTTCAT CTTGGATGGG 
AAGCTCGCCA CCATGTACTC GAGTGGGAGC CCAGAGGGGG GGTCAGACAG CTCAGAGTCT 
CGCAGTGAGT TTTTGGAGAA GCTGCAGCGT GCCAGAAGTC AGGTGAAACC AGCAACAGCC 
AGCCAGCCCA TCCTCTCCAT GGTCGGACCC ACCAAGCTGA CGGTGGGGAA CTGGTCTCTG 
ACCTGCCTGA AGGAAGGCGA GATTGCAATT CACAACTCAG ACGGGCAGCA GGCCACGATC 
CTGAAGGAGG ATCTGCCCGG CTTCGTGTTC GAGTCCAACA GAGGAACCAA ACACTCGTTC 
ACTGCTGAAA CGTCGCTCGG TTCTGAGTTT GTCACTGGCT GGACGGGGAA ACGTGGCAGA 
AAGCTGAAGT CCAAGCTGGA GAAGACCAAG CAGAAGGTGA AGAGCATGGC CAGGGAGCTG 
TACGACGACC ACTTCAAAGC TGTGGAGAGC ATGCCCAGAG GAGTGGTGGT CACCCTGAGG 
AACATCGCCA CGCAGCTGGA GTCCGCCTGG GAGCTGCACA TAAACCGACA GTGTCTGGAA 
GGAGAGAACA CGTGGAGGGA CCTGATGAAG ACCGCTCTGG AGAACCTGAT TGTGGTTTTG 
AAGGATGAAA ACACGATTTC TCCGTATGAA ATGTGCAGCA GCGGCCTGGT TCAGGCTCTG 
TTTACCGTCC TCAGCAACAG TGTGGAGCTG GACCTGAAAC ATGACTGTAA GCCTCTAATG 
GAAAGGATCA ACGTCTTTAA AGCTGCTTTC AGCGAGAATG AAGACAATGA AAGCCGACCA 
GCCGTTGCCT TAGTGCGGAA GCTGATAGCC GTCCTGGAAT CGATCGAGCG TCTGCCCCTG 
CACCTGTATG ACACGCCTGG ATCCTCCTAC AATCTGCAGA TTCTGACGAG GAGGCTGCGG 
TTCCGTCTGG AGCGAGCGCC TGGGGAGACC GCTCTCATTG ACCGGACGGG ACGCATGCTG 
AAGATGGAGC CGCTCGCCAC CGTAGAGTCT CTGGAGCAGT ACCTGCTGAA AATGGTCGCT 
AAACAGTGGT ACGACTTTGA ACGCTCATCC TTCGTTTTTG TGAGAAAACT GAGAGAAGGA 
CAGATCTTCA CCTTCAGGCA CCAGCACGAC TTTGACGAGA ACGGCATCAT CTACTGGGTC 
GGAACCAACG CCAAGACGGC GTACGAGTGG GTGAACCCCG CAGCGTACGG CCTGGTGGTG 
GTGACCTCCT CCGAGGGGAG GAATCTGCCT TATGGGCGTT TGGAGGACAT CCTGAGTCGG 
GATAGCTCCG CCCTCAACTG CCACACGAAC GACGACAAGA ACGCCTGGTT CGCAGTTGAC 
CTCGGCCTCT GGGTGATTCC GTCAGCGTAC ACTCTGAGGC ACGCCAGGGG GTACGGACGC 
TCTGCGCTCA GGAACTGGGT TTTCCAAGTG TCCAAGGACG GACAGAACTG GTCCACACTC 
TACAACCACA TCGACGATGG CAGTCTCAAT GAACCGGGGT CTACGGCCAC GTGGCCTCTG 
GACCCGTCCA AAGAGGAGAA GCAGGGCTGG AGACACATCC GCATCAAGCA GATGGGGAAG 
AACGCCAGCG GTCAGACACA CTACCTGTCC TTATCTGGGC TGGAGCTGTA CGGCACGGTC 
ACCGCCGTAT GCGAGGACCA GCTGGGTAAA GCTGTGAAAG AGGCGGAGGC CAACCTTCGT 
CGCCAGCGGC GCCTCTTCCG TTCTCAGGTC ATGAAGTACA TCGTCCCAGG TGCGCGGGTC 
ATCCGTGGCA TCGACTGGAA GTGGAGGGAC CAGGATGGAA ACCCGGCAGG GGAAGGCACT 
GTCACTGGGG AGGCGCACAA CGGCTGGATT GATGTAACCT GGGATGCTGG CGGCTCTAAC 
TCTTATCGTA TGGGCGCCGA AGGGAAGTTT GACCTCAAGC TTGCTCCAGG GTACGACCCT 
GAGCTGGCTG CCACAGCGCC ATCACCCAAA CCTGTCTCAT CCGCTGTTTC AGGTCCCGCC 
TCCTCCACGG TGGGACCCTC TGCGACGCCA GCAGCTGTTG GCGGAACCAC CATCACCACC 
TCATCCTCCT CCACCACCTC ATCGTCCTCG CAGCAGCCTT CCTGGAGCAG TTTGGTGAAA 
AATAACTGTC CCGACAAGGG AGGGGCCAGC TCCTCCAGCA GGAAGGGTAG CAGCAGCTCC 
GTCTGCAGCG TCGCTTCCTC CTCTGACATC AGTCTGAGCT CCTCCATTGG GCTGCCTGGC 
GCTGGGAGCC TGCAGCTGGA CAGGAAGGCC GAGGGGCTGC TGCTAGATCA GGCCGCGGGG 
GTGGAAGGGG TGACGGGGGG AGGGACCGGT TCCGATGGAC ATCAGCAAGA GCCGATAGTC 
GTTCTGTCCT CCGTGACCGA ATCCGGTTCG GCGTCCAGCT CCGGCACGCT CACCGCCGAC 
GTGTCAACGG CGGGTGACGA GCATCACTCC CCCTCTGCAA TGGCCACTGC TGTGGACCCG 
GCCACGGCCA TCTCCATGGG CTTGGTGAGC GTCAGCTCCC CGGACGTCAG CTCCGTGTCG 
GAGCCCTCCA GCAAAGACAC TCACTCCCAG AGGCCGCTGT GCTCGGCCGT GAACACCCGG 
CTGTCCGTCA GCTCCCTGCT GGCCGCCAGC GCTCCCATGA GCTCCAGCGC CAGCGTGCCC 
AATCTGTCCT CTCGGGAGGC CAGCCTCATG GAGTCCTTCG TCCGCCGCGC GCCCAACATG 
TCCCGCACCA ACGCTACCAA CAACATGAAC CTGAGCCGCA GCAGCAGCGA CAACAACACC 
AACACACTGG GCAGGAACGT GATGAGCACA GCCACATCTC CTCTTATGGG CGCTCAGAGC 
TTTCCTAACC TCACCACCAC TGGCACCACC TCCACCGTTA CCATGTCAAC CTCCATAGTA 
ACCTGCGGTA ATAATGTAGC CACAGCCACC ACGGGGCTGT CTGTGGGACA GTTGCTAAGC 
AACACACTGA CAACCAGCTT AACCTCCACA TCCAGCGAGA GCGACACGGG ACAGGAGGCC 
GAGTTCTCCC TCTATGACTT CCTGGACAGT TGCCGGGCCA ACACGCTGCT AGCAGAGCTG 
GACGATGAGG AGGACCTCCC AGAGCCCGAC GACGACGACG ATGAGAATGA AGACGACAAT 
CAGGAGGATC AGGAGTATGA GGAGGAGGAG GAGGAGTATG AGACGAAGGG AGGACGGAGG 
AGGACATGGG ACGATGACTT TGTCCTAAAA AGACAGTTTT CTGCTTTGGT CCCTGCCTTC 
GACCCCAGAC CAGGAAGAAC CAACGTCCAG CAGACCACCG ACCTGGAGAT CCCCCCACCA 
GGAACTCCGC GGTCGGAGGT TCGGGAGGAG GTGGAGTGTG CCCCATCCCC CCACCTCGCC 
CTCACACTGA AGGTGGCGGG TCTCGGCACG GCTCGGGAGG TGGAGATCCC GCTCTCCAAC 
TACAAGTTGA CCATCTTCTA CTATGTGCAG CGGCTCCTGC AGCTCTCCTG CAACGGCGCC 
GTAAAGACGG ACAAGCTGCG GCGCATCTGG GAGCCGACGT ACACGATCAT GTACAGAGAG 
CTCAAAGACT CGGACAAAGA GAAGGAGAGC GGGAAGATGG ACCTCTTTGA GCACGTTGTT 
GGAAGTCGTT CTGGAGGTCT GAGCCCCAGC TCGGGTTCAG CCAATCAGAG CAGCGAGATC 
CTGGGCTGGG CGAAGGAGGC GGTCCTGGCC AAGGCGGGCT GCAGCCAGAA CGCCTGCGGG 
GTGGAAGACG TCCTGCAGCT GCTGCGCATT CTCTATGTGA TTGGGAGAGA CTCTACTGCA 
GGCGGACGCA CGCTGCAAGA GGATGTTGAC GAGCTGCAGT TCAACTCGCC TCCTGAGGAG 
TTCAGCAGCA AGAAGATCAC CACCAAGATC CTGCAGCAGA TCGAGGAGCC CTTGGCTCTG 
GCTAGCGGAG CGCTGCCGGA CTGGTGTGAG CAGCTCACCT CCAAGTGTCC TTTCCTGATC 
CCCTTTGAGA CCAGACAGCT GTACTTCACC TGCACTGCCT TTGGGGCCTC CAGGGCGATC 
GTGTGGCTCC AGAACCGCCG CGAGGCGACC ATGGAGCGCT CACGGCCATC CACCACCGTC 
CGGCGAGACG ACCCCGGAGA GTTCAGAGTC GGTCGGCTTA AACACGAGCG CGTTAAAGTT 
CCTCGAGGGG AGTCCATGAT GGAGTGGGCG GAGTCTGTGA TGCAGATCCA CGCAAACAGG 
AAGTCTGTTT TAGAGGTGGA GTTTCTGGGT GAGGAGGGGA CGGGTCTGGG TCCGACTCTG 
GAGTTCTACG CTCTGGTGGC GGCGGAGTTT CAGAGAACGT CTCTGGGGAT CTGGCTCTGT 
GACGACGACT TCCCGGACGA CGAGTCCCGA CAGGTGGATC TGGGTGGAGG TCTGAAGCCT 
CCTGGCTTCT ACGTGCAGCG GTCCTGCGGG CTCTTCCCGG CGCCGTTCCC TCAGGACAGC 
GAGGAGCTGG AGCGGATTAC GCGGCTCTTC CACTTCCTGG GGATCTTCCT GGCCAAGTGC 
ATCCAGGACA ACCGGCTGGT GGACCTGCCC GTGTCCCAGC CTTTCTTCAA GCTGCTCTGC 
ATGGGGGACA TCAAGTCCAA CATGAGCAAG CTGCTGTACC AGTCCCGCGG CTCCCCCCAG 
GGCCACCTCT CCGAGAGGGC CCCCCTGCTG CTGCTGACGG AGGTGCAGTC GGAGGCGTCC 
ACAGAGGAGA GCCAGGAGAC CTACTCTGTG GGGAGCTTCG ACGAGGACTC CAAGTCAGAC 
TTCATCATGG ACCCCCCCAA ACCCAAGCCG CCCGCCTGGT TCCACGGCAT CATGTCCTGG 
GACGACTTCC ATCTGGTCAA CCCGCACAGA GCCAGTTTCC TGAAGGAGGT GAAGGAGCTG 
GCAGTGAGGA GGAGACAGAT TCTGACCAGC AAGAGTTTGT CAGAGGATGA GAAGAACACC 
AGGCTGCAGG ACCTGATGCT GAGGAACCCG CTGGGCTCTG GGCCCCCCTT AAGCGTCGAA 
GACCTCGGGT TGAACTTCCA GTTCTGTCCG TCCTCCAAGA TTCACGGCTT CTTCGCGGTG 
GATCTGAAAC CAAACGGGGA TGACGAGATG GTGAGTCTGG AGAACGCCGA GGAGTACGTG 
GAGCTGATGT TCGACTTCTG CATGCACGCC GGCATCCAGA AGCAGATGGA GGCGTTCAGA 
GAGGGTTTCA ACCGCGTCTT CCCCATGGAG AAGCTCAGCT CGTTCAGCCA GAAGGAGGTG 
CAGATGATCC TCTGCGGAAA CCAATCCCCC TGCTGGACCG CCGATGACAT CATCAGCTAC 
ACCGAGCCAA AGCTGGGATA CACCAGAGAC AGCCCTGGGT TCCTGCGCTT CATCCGAGTT 
TTATGCGGAA TGTCGACGGA CGAGAGGAAA GCTTTCCTGC AGTTCACCAC CGGCTGCTCC 
ACGCTGCCCC CCGGTGGCCT GGCTAACCTG CACCCGCGCC TCACCATCGT CAGAAAGGTG 
GACGCCACGG ACTCCAGCTA CCCATCGGTG AACACGTGCG TTCACTACCT GAAGCTGCCG 
GAGTACTCGT CAGAGGAGAT CATGAGGGAG CGGCTGTTGG CGGCCACCAT GGAGAAGGGC 
TTCCACCTCA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hectd1 ( XM_020713930.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020569589.1 Oryzias latipes HECT domain E3 ubiquitin protein ligase 1 (hectd1), transcript variant X3, mRNA.

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CloneID	OJa76016
Clone ID Related Accession (Same CDS sequence)	XM_020713930.2 , XM_020713930.1
Accession Version	XM_020713930.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_020713930.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG TGGACCCAGA CACGTTGCTG GAGTGGCTGC AGATGGGACA GGGCGATGAG 
CGGGACATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGCTGCTCAT GTCGGACAAC 
GTGGACCGCT GCTTTGAGAC ATGTCCTCCT CGAACGTTCC TCCCAGCGCT TTGTAAGATC 
TTCTTGGACG AGAGCGCTCC TGATAACGTG CTGGAGGTCA CGGCCAGAGC CATCACCTAC 
TACCTGGACG TGTCGGCAGA ATGCACGCGG AGGATCGTGG GTGTGGACGG GGCCATAAAG 
GCCCTGTGCA ACCGGCTGGT GGTGGTGGAA CTCAACAACA GGACCAGCAG GGATCTGGCT 
GAGCAGTGTG TTAAGGTGCT GGAGCTGATC TGTACCAGGG AATCTGGCGC CGTGTTTGAA 
GCCAGCGGTC TGAACTGCGT TTTGAGCTTC ATACGCGACA GTGGTCACCT GGTCCACAAA 
GACACTCTGC ACTCTGCAAT GGCAGTGGTG TCTCGCCTCT GTAGCAAGAT GGAGCCGCAG 
GACTCTTCTC TAGAAACCTG CGTGGAGTCT CTGTCCAGCC TCCTGAAACA TGAGGATCAC 
CAGGTGTCAG ACGGTGCTCT GCGTTGCTTC GCCTCATTGG CTGATCGGTT TACTCGTCGA 
GGCGTAGACC CCGCCCCTCT GGCCAAACAT GGACTGACGG AGGAGCTGCT TTCCCGCATG 
GCGGCTGCCG GAGGTTCCGT GTCGGGCTCT TCTTCTGCCT GTAAACCAGG TCGGCCCTCC 
ACTGGTGCCA CACCGTCTGC CTCCGACTCC AAACTGAGCA ACCAGGTGTC CACCATCGTT 
AGCCTGCTGT CCACGCTCTG CCGGGGGTCG CCAGTGGTCA CTCATGACTT GTTACGTTCG 
GCACTCCCTA ACTCCATGGA GTCTGCGCTG GGAGGAGACG AGCGCTGTGT GCTGGACACC 
ATGCGGCTGG TCGACCTGCT GCTGGTGCTT CTGTTCGAGG GGCGGAAAGC GCTGCCAAAG 
TCCACCGCCG GCTCAGCAGG TCGGATCCCG GGGCTTCGCC GGCTGGACAG CTCAGGGGAG 
CGGTCGCACC GACAGCTCAT CGACTGCATC CGCAGCAAAG ACACGGACGC CCTGATAGAT 
GCCATCGACA CTGGAGCTTT TGAAGTGAAC TTCATGGACG ACGTGGGACA GACGCTGCTG 
AACTGGGCCT CAGCTTTTGG AACACAGGAA ATGGTGGAGT TTTTGTGCGA GAGGGGTGCA 
GATGTGAACC GAGGCCAGAG GTCATCCTCG CTGCACTACG CTGCCTGTTT CGGACGGCCA 
CAAGTTGCCA AGACGCTGCT GCGGCACGGA GCCAACCCGG ACCTGAGGGA TGAGGACGGG 
AAGACTCCTT TGGACAAAGC CAGGGAGAGG GGACACAGTG AGGTGGTGGC CATCCTTCAG 
TCTCCGGGAG ACTGGATGTG TCCAGTGAAC AAAGGAGACG ACAAGAGGAA GAAAGATGCG 
AGTAAGGAAG AAGAGGAGGG CAGTGAACCC AAAGGAGACC CAGAAATGGC TCCTTTCTAC 
CTGAAAAGAC TCCTCCCTGT TTTTGCACAA ACCTTTCAGC AAACCATGCT GCCTTCCATC 
AGGAAAGCCA GTCTGGCCCT CATCAGGAAG ATGGTTCACT ACAGCAGCGA GGTTCTGCTG 
AGGGAGGTGT GCGACAGTGA GACGGGACAC AACCTGCCCA CAGTGCTGGT GGAGATCACC 
GCCACCGTCC TCGACCAGGA GGATGATGAC GACGGTCACC TGCTGGCTCT GCAGATCATC 
AGGGATCTGG TGGACAAAGG TGGAGATGTT TTCCTTGACC AGCTGGCCCG ACTCGGGGTC 
ATCAACAAGG TGTCGACCTT GGCCGGACCG ACGTCTGATG ATGAGAACGA GGATGAAGTC 
AAACCTGAAA AGGAGGAGGA GGTGCAGGAA GATGCAAAGG AAATCCAGCA GGGGAAGCCG 
TACCACTGGA GGGACTGGTC CATCATCAGA GGCAGGGACT GCCTCTACAT TTGGTCAGAC 
GCCGCCGCCC TCGAGCTCTC CAACGGCTCC AATGGCTGGT TTCGCTTCAT CTTGGATGGG 
AAGCTCGCCA CCATGTACTC GAGTGGGAGC CCAGAGGGGG GGTCAGACAG CTCAGAGTCT 
CGCAGTGAGT TTTTGGAGAA GCTGCAGCGT GCCAGAAGTC AGGTGAAACC AGCAACAGCC 
AGCCAGCCCA TCCTCTCCAT GGTCGGACCC ACCAAGCTGA CGGTGGGGAA CTGGTCTCTG 
ACCTGCCTGA AGGAAGGCGA GATTGCAATT CACAACTCAG ACGGGCAGCA GGCCACGATC 
CTGAAGGAGG ATCTGCCCGG CTTCGTGTTC GAGTCCAACA GAGGAACCAA ACACTCGTTC 
ACTGCTGAAA CGTCGCTCGG TTCTGAGTTT GTCACTGGCT GGACGGGGAA ACGTGGCAGA 
AAGCTGAAGT CCAAGCTGGA GAAGACCAAG CAGAAGGTGA AGAGCATGGC CAGGGAGCTG 
TACGACGACC ACTTCAAAGC TGTGGAGAGC ATGCCCAGAG GAGTGGTGGT CACCCTGAGG 
AACATCGCCA CGCAGCTGGA GTCCGCCTGG GAGCTGCACA TAAACCGACA GTGTCTGGAA 
GGAGAGAACA CGTGGAGGGA CCTGATGAAG ACCGCTCTGG AGAACCTGAT TGTGGTTTTG 
AAGGATGAAA ACACGATTTC TCCGTATGAA ATGTGCAGCA GCGGCCTGGT TCAGGCTCTG 
TTTACCGTCC TCAGCAACAG TGTGGAGCTG GACCTGAAAC ATGACTGTAA GCCTCTAATG 
GAAAGGATCA ACGTCTTTAA AGCTGCTTTC AGCGAGAATG AAGACAATGA AAGCCGACCA 
GCCGTTGCCT TAGTGCGGAA GCTGATAGCC GTCCTGGAAT CGATCGAGCG TCTGCCCCTG 
CACCTGTATG ACACGCCTGG ATCCTCCTAC AATCTGCAGA TTCTGACGAG GAGGCTGCGG 
TTCCGTCTGG AGCGAGCGCC TGGGGAGACC GCTCTCATTG ACCGGACGGG ACGCATGCTG 
AAGATGGAGC CGCTCGCCAC CGTAGAGTCT CTGGAGCAGT ACCTGCTGAA AATGGTCGCT 
AAACAGTGGT ACGACTTTGA ACGCTCATCC TTCGTTTTTG TGAGAAAACT GAGAGAAGGA 
CAGATCTTCA CCTTCAGGCA CCAGCACGAC TTTGACGAGA ACGGCATCAT CTACTGGGTC 
GGAACCAACG CCAAGACGGC GTACGAGTGG GTGAACCCCG CAGCGTACGG CCTGGTGGTG 
GTGACCTCCT CCGAGGGGAG GAATCTGCCT TATGGGCGTT TGGAGGACAT CCTGAGTCGG 
GATAGCTCCG CCCTCAACTG CCACACGAAC GACGACAAGA ACGCCTGGTT CGCAGTTGAC 
CTCGGCCTCT GGGTGATTCC GTCAGCGTAC ACTCTGAGGC ACGCCAGGGG GTACGGACGC 
TCTGCGCTCA GGAACTGGGT TTTCCAAGTG TCCAAGGACG GACAGAACTG GTCCACACTC 
TACAACCACA TCGACGATGG CAGTCTCAAT GAACCGGGGT CTACGGCCAC GTGGCCTCTG 
GACCCGTCCA AAGAGGAGAA GCAGGGCTGG AGACACATCC GCATCAAGCA GATGGGGAAG 
AACGCCAGCG GTCAGACACA CTACCTGTCC TTATCTGGGC TGGAGCTGTA CGGCACGGTC 
ACCGCCGTAT GCGAGGACCA GCTGGGTAAA GCTGTGAAAG AGGCGGAGGC CAACCTTCGT 
CGCCAGCGGC GCCTCTTCCG TTCTCAGGTC ATGAAGTACA TCGTCCCAGG TGCGCGGGTC 
ATCCGTGGCA TCGACTGGAA GTGGAGGGAC CAGGATGGAA ACCCGGCAGG GGAAGGCACT 
GTCACTGGGG AGGCGCACAA CGGCTGGATT GATGTAACCT GGGATGCTGG CGGCTCTAAC 
TCTTATCGTA TGGGCGCCGA AGGGAAGTTT GACCTCAAGC TTGCTCCAGG GTACGACCCT 
GAGCTGGCTG CCACAGCGCC ATCACCCAAA CCTGTCTCAT CCGCTGTTTC AGGTCCCGCC 
TCCTCCACGG TGGGACCCTC TGCGACGCCA GCAGCTGTTG GCGGAACCAC CATCACCACC 
TCATCCTCCT CCACCACCTC ATCGTCCTCG CAGCAGCCTT CCTGGAGCAG TTTGGTGAAA 
AATAACTGTC CCGACAAGGG AGGGGCCAGC TCCTCCAGCA GGAAGGGTAG CAGCAGCTCC 
GTCTGCAGCG TCGCTTCCTC CTCTGACATC AGTCTGAGCT CCTCCATTGG GCTGCCTGGC 
GCTGGGAGCC TGCAGCTGGA CAGGAAGGCC GAGGGGCTGC TGCTAGATCA GGCCGCGGGG 
GTGGAAGGGG TGACGGGGGG AGGGACCGGT TCCGATGGAC ATCAGCAAGA GCCGATAGTC 
GTTCTGTCCT CCGTGACCGA ATCCGGTTCG GCGTCCAGCT CCGGCACGCT CACCGCCGAC 
GTGTCAACGG CGGGTGACGA GCATCACTCC CCCTCTGCAA TGGCCACTGC TGTGGACCCG 
GCCACGGCCA TCTCCATGGG CTTGGTGAGC GTCAGCTCCC CGGACGTCAG CTCCGTGTCG 
GAGCCCTCCA GCAAAGACAC TCACTCCCAG AGGCCGCTGT GCTCGGCCGT GAACACCCGG 
CTGTCCGTCA GCTCCCTGCT GGCCGCCAGC GCTCCCATGA GCTCCAGCGC CAGCGTGCCC 
AATCTGTCCT CTCGGGAGGC CAGCCTCATG GAGTCCTTCG TCCGCCGCGC GCCCAACATG 
TCCCGCACCA ACGCTACCAA CAACATGAAC CTGAGCCGCA GCAGCAGCGA CAACAACACC 
AACACACTGG GCAGGAACGT GATGAGCACA GCCAACTTCC TGGACAGTTG CCGGGCCAAC 
ACGCTGCTAG CAGAGCTGGA CGATGAGGAG GACCTCCCAG AGCCCGACGA CGACGACGAT 
GAGAATGAAG ACGACAATCA GGAGGATCAG GAGTATGAGG AGGTCCTGGA GGAGGAGGAG 
TATGAGACGA AGGGAGGACG GAGGAGGACA TGGGACGATG ACTTTGTCCT AAAAAGACAG 
TTTTCTGCTT TGGTCCCTGC CTTCGACCCC AGACCAGGAA GAACCAACGT CCAGCAGACC 
ACCGACCTGG AGATCCCCCC ACCAGGAACT CCGCGGTCGG AGGTTCGGGA GGAGGTGGAG 
TGTGCCCCAT CCCCCCACCT CGCCCTCACA CTGAAGGTGG CGGGTCTCGG CACGGCTCGG 
GAGGTGGAGA TCCCGCTCTC CAACTACAAG TTGACCATCT TCTACTATGT GCAGCGGCTC 
CTGCAGCTCT CCTGCAACGG CGCCGTAAAG ACGGACAAGC TGCGGCGCAT CTGGGAGCCG 
ACGTACACGA TCATGTACAG AGAGCTCAAA GACTCGGACA AAGAGAAGGA GAGCGGGAAG 
ATGGACCTCT TTGAGCACGT TGTTGGAAGT CGTTCTGGAG GTCTGAGCCC CAGCTCGGGT 
TCAGCCAATC AGAGCAGCGA GATCCTGGGC TGGGCGAAGG AGGCGGTCCT GGCCAAGGCG 
GGCTGCAGCC AGAACGCCTG CGGGGTGGAA GACGTCCTGC AGCTGCTGCG CATTCTCTAT 
GTGATTGGGA GAGACTCTAC TGCAGGCGGA CGCACGCTGC AAGAGGATGT TGACGAGCTG 
CAGTTCAACT CGCCTCCTGA GGAGTTCAGC AGCAAGAAGA TCACCACCAA GATCCTGCAG 
CAGATCGAGG AGCCCTTGGC TCTGGCTAGC GGAGCGCTGC CGGACTGGTG TGAGCAGCTC 
ACCTCCAAGT GTCCTTTCCT GATCCCCTTT GAGACCAGAC AGCTGTACTT CACCTGCACT 
GCCTTTGGGG CCTCCAGGGC GATCGTGTGG CTCCAGAACC GCCGCGAGGC GACCATGGAG 
CGCTCACGGC CATCCACCAC CGTCCGGCGA GACGACCCCG GAGAGTTCAG AGTCGGTCGG 
CTTAAACACG AGCGCGTTAA AGTTCCTCGA GGGGAGTCCA TGATGGAGTG GGCGGAGTCT 
GTGATGCAGA TCCACGCAAA CAGGAAGTCT GTTTTAGAGG TGGAGTTTCT GGGTGAGGAG 
GGGACGGGTC TGGGTCCGAC TCTGGAGTTC TACGCTCTGG TGGCGGCGGA GTTTCAGAGA 
ACGTCTCTGG GGATCTGGCT CTGTGACGAC GACTTCCCGG ACGACGAGTC CCGACAGGTG 
GATCTGGGTG GAGGTCTGAA GCCTCCTGGC TTCTACGTGC AGCGGTCCTG CGGGCTCTTC 
CCGGCGCCGT TCCCTCAGGA CAGCGAGGAG CTGGAGCGGA TTACGCGGCT CTTCCACTTC 
CTGGGGATCT TCCTGGCCAA GTGCATCCAG GACAACCGGC TGGTGGACCT GCCCGTGTCC 
CAGCCTTTCT TCAAGCTGCT CTGCATGGGG GACATCAAGT CCAACATGAG CAAGCTGCTG 
TACCAGTCCC GCGGCTCCCC CCAGGGCCAC CTCTCCGAGA GGGCCCCCCT GCTGCTGCTG 
ACGGAGGTGC AGTCGGAGGC GTCCACAGAG GAGAGCCAGG AGACCTACTC TGTGGGGAGC 
TTCGACGAGG ACTCCAAGTC AGACTTCATC ATGGACCCCC CCAAACCCAA GCCGCCCGCC 
TGGTTCCACG GCATCATGTC CTGGGACGAC TTCCATCTGG TCAACCCGCA CAGAGCCAGT 
TTCCTGAAGG AGGTGAAGGA GCTGGCAGTG AGGAGGAGAC AGATTCTGAC CAGCAAGAGT 
TTGTCAGAGG ATGAGAAGAA CACCAGGCTG CAGGACCTGA TGCTGAGGAA CCCGCTGGGC 
TCTGGGCCCC CCTTAAGCGT CGAAGACCTC GGGTTGAACT TCCAGTTCTG TCCGTCCTCC 
AAGATTCACG GCTTCTTCGC GGTGGATCTG AAACCAAACG GGGATGACGA GATGGTGAGT 
CTGGAGAACG CCGAGGAGTA CGTGGAGCTG ATGTTCGACT TCTGCATGCA CGCCGGCATC 
CAGAAGCAGA TGGAGGCGTT CAGAGAGGGT TTCAACCGCG TCTTCCCCAT GGAGAAGCTC 
AGCTCGTTCA GCCAGAAGGA GGTGCAGATG ATCCTCTGCG GAAACCAATC CCCCTGCTGG 
ACCGCCGATG ACATCATCAG CTACACCGAG CCAAAGCTGG GATACACCAG AGACAGCCCT 
GGGTTCCTGC GCTTCATCCG AGTTTTATGC GGAATGTCGA CGGACGAGAG GAAAGCTTTC 
CTGCAGTTCA CCACCGGCTG CTCCACGCTG CCCCCCGGTG GCCTGGCTAA CCTGCACCCG 
CGCCTCACCA TCGTCAGAAA GGTGGACGCC ACGGACTCCA GCTACCCATC GGTGAACACG 
TGCGTTCACT ACCTGAAGCT GCCGGAGTAC TCGTCAGAGG AGATCATGAG GGAGCGGCTG 
TTGGCGGCCA CCATGGAGAA GGGCTTCCAC CTCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020569589.1
CDS	460..7938
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEASGLNCVLSFIRDSGHLVHKDTLHSAMAVVSRLCSKMEPQDSSLETCVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGSVSGSSSACKPGRPSTGATPSASDSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSALPNSMESALGGDERCVLDTMRLVDLLLVLLFEGRKALPKSTAGSAGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKRKKDASKEEEEGSEPKGDPEMAPFYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMVHYSSEVLLREVCDSETGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDVFLDQLARLGVINKVSTLAGPTSDDENEDEVKPEKEEEVQEDAKEIQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARSQVKPATASQPILSMVGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVKSMARELYDDHFKAVESMPRGVVVTLRNIATQLESAWELHINRQCLEGENTWRDLMKTALENLIVVLKDENTISPYEMCSSGLVQALFTVLSNSVELDLKHDCKPLMERINVFKAAFSENEDNESRPAVALVRKLIAVLESIERLPLHLYDTPGSSYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFERSSFVFVRKLREGQIFTFRHQHDFDENGIIYWVGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDSSALNCHTNDDKNAWFAVDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWSTLYNHIDDGSLNEPGSTATWPLDPSKEEKQGWRHIRIKQMGKNASGQTHYLSLSGLELYGTVTAVCEDQLGKAVKEAEANLRRQRRLFRSQVMKYIVPGARVIRGIDWKWRDQDGNPAGEGTVTGEAHNGWIDVTWDAGGSNSYRMGAEGKFDLKLAPGYDPELAATAPSPKPVSSAVSGPASSTVGPSATPAAVGGTTITTSSSSTTSSSSQQPSWSSLVKNNCPDKGGASSSSRKGSSSSVCSVASSSDISLSSSIGLPGAGSLQLDRKAEGLLLDQAAGVEGVTGGGTGSDGHQQEPIVVLSSVTESGSASSSGTLTADVSTAGDEHHSPSAMATAVDPATAISMGLVSVSSPDVSSVSEPSSKDTHSQRPLCSAVNTRLSVSSLLAASAPMSSSASVPNLSSREASLMESFVRRAPNMSRTNATNNMNLSRSSSDNNTNTLGRNVMSTANFLDSCRANTLLAELDDEEDLPEPDDDDDENEDDNQEDQEYEEVLEEEEYETKGGRRRTWDDDFVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPRSEVREEVECAPSPHLALTLKVAGLGTAREVEIPLSNYKLTIFYYVQRLLQLSCNGAVKTDKLRRIWEPTYTIMYRELKDSDKEKESGKMDLFEHVVGSRSGGLSPSSGSANQSSEILGWAKEAVLAKAGCSQNACGVEDVLQLLRILYVIGRDSTAGGRTLQEDVDELQFNSPPEEFSSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATMERSRPSTTVRRDDPGEFRVGRLKHERVKVPRGESMMEWAESVMQIHANRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTSLGIWLCDDDFPDDESRQVDLGGGLKPPGFYVQRSCGLFPAPFPQDSEELERITRLFHFLGIFLAKCIQDNRLVDLPVSQPFFKLLCMGDIKSNMSKLLYQSRGSPQGHLSERAPLLLLTEVQSEASTEESQETYSVGSFDEDSKSDFIMDPPKPKPPAWFHGIMSWDDFHLVNPHRASFLKEVKELAVRRRQILTSKSLSEDEKNTRLQDLMLRNPLGSGPPLSVEDLGLNFQFCPSSKIHGFFAVDLKPNGDDEMVSLENAEEYVELMFDFCMHAGIQKQMEAFREGFNRVFPMEKLSSFSQKEVQMILCGNQSPCWTADDIISYTEPKLGYTRDSPGFLRFIRVLCGMSTDERKAFLQFTTGCSTLPPGGLANLHPRLTIVRKVDATDSSYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_020713930.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes HECT domain E3 ubiquitin protein ligase 1 (hectd1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020713930.2

ORF Insert Sequence:

ATGGCGGATG TGGACCCAGA CACGTTGCTG GAGTGGCTGC AGATGGGACA GGGCGATGAG 
CGGGACATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGCTGCTCAT GTCGGACAAC 
GTGGACCGCT GCTTTGAGAC ATGTCCTCCT CGAACGTTCC TCCCAGCGCT TTGTAAGATC 
TTCTTGGACG AGAGCGCTCC TGATAACGTG CTGGAGGTCA CGGCCAGAGC CATCACCTAC 
TACCTGGACG TGTCGGCAGA ATGCACGCGG AGGATCGTGG GTGTGGACGG GGCCATAAAG 
GCCCTGTGCA ACCGGCTGGT GGTGGTGGAA CTCAACAACA GGACCAGCAG GGATCTGGCT 
GAGCAGTGTG TTAAGGTGCT GGAGCTGATC TGTACCAGGG AATCTGGCGC CGTGTTTGAA 
GCCAGCGGTC TGAACTGCGT TTTGAGCTTC ATACGCGACA GTGGTCACCT GGTCCACAAA 
GACACTCTGC ACTCTGCAAT GGCAGTGGTG TCTCGCCTCT GTAGCAAGAT GGAGCCGCAG 
GACTCTTCTC TAGAAACCTG CGTGGAGTCT CTGTCCAGCC TCCTGAAACA TGAGGATCAC 
CAGGTGTCAG ACGGTGCTCT GCGTTGCTTC GCCTCATTGG CTGATCGGTT TACTCGTCGA 
GGCGTAGACC CCGCCCCTCT GGCCAAACAT GGACTGACGG AGGAGCTGCT TTCCCGCATG 
GCGGCTGCCG GAGGTTCCGT GTCGGGCTCT TCTTCTGCCT GTAAACCAGG TCGGCCCTCC 
ACTGGTGCCA CACCGTCTGC CTCCGACTCC AAACTGAGCA ACCAGGTGTC CACCATCGTT 
AGCCTGCTGT CCACGCTCTG CCGGGGGTCG CCAGTGGTCA CTCATGACTT GTTACGTTCG 
GCACTCCCTA ACTCCATGGA GTCTGCGCTG GGAGGAGACG AGCGCTGTGT GCTGGACACC 
ATGCGGCTGG TCGACCTGCT GCTGGTGCTT CTGTTCGAGG GGCGGAAAGC GCTGCCAAAG 
TCCACCGCCG GCTCAGCAGG TCGGATCCCG GGGCTTCGCC GGCTGGACAG CTCAGGGGAG 
CGGTCGCACC GACAGCTCAT CGACTGCATC CGCAGCAAAG ACACGGACGC CCTGATAGAT 
GCCATCGACA CTGGAGCTTT TGAAGTGAAC TTCATGGACG ACGTGGGACA GACGCTGCTG 
AACTGGGCCT CAGCTTTTGG AACACAGGAA ATGGTGGAGT TTTTGTGCGA GAGGGGTGCA 
GATGTGAACC GAGGCCAGAG GTCATCCTCG CTGCACTACG CTGCCTGTTT CGGACGGCCA 
CAAGTTGCCA AGACGCTGCT GCGGCACGGA GCCAACCCGG ACCTGAGGGA TGAGGACGGG 
AAGACTCCTT TGGACAAAGC CAGGGAGAGG GGACACAGTG AGGTGGTGGC CATCCTTCAG 
TCTCCGGGAG ACTGGATGTG TCCAGTGAAC AAAGGAGACG ACAAGAGGAA GAAAGATGCG 
AGTAAGGAAG AAGAGGAGGG CAGTGAACCC AAAGGAGACC CAGAAATGGC TCCTTTCTAC 
CTGAAAAGAC TCCTCCCTGT TTTTGCACAA ACCTTTCAGC AAACCATGCT GCCTTCCATC 
AGGAAAGCCA GTCTGGCCCT CATCAGGAAG ATGGTTCACT ACAGCAGCGA GGTTCTGCTG 
AGGGAGGTGT GCGACAGTGA GACGGGACAC AACCTGCCCA CAGTGCTGGT GGAGATCACC 
GCCACCGTCC TCGACCAGGA GGATGATGAC GACGGTCACC TGCTGGCTCT GCAGATCATC 
AGGGATCTGG TGGACAAAGG TGGAGATGTT TTCCTTGACC AGCTGGCCCG ACTCGGGGTC 
ATCAACAAGG TGTCGACCTT GGCCGGACCG ACGTCTGATG ATGAGAACGA GGATGAAGTC 
AAACCTGAAA AGGAGGAGGA GGTGCAGGAA GATGCAAAGG AAATCCAGCA GGGGAAGCCG 
TACCACTGGA GGGACTGGTC CATCATCAGA GGCAGGGACT GCCTCTACAT TTGGTCAGAC 
GCCGCCGCCC TCGAGCTCTC CAACGGCTCC AATGGCTGGT TTCGCTTCAT CTTGGATGGG 
AAGCTCGCCA CCATGTACTC GAGTGGGAGC CCAGAGGGGG GGTCAGACAG CTCAGAGTCT 
CGCAGTGAGT TTTTGGAGAA GCTGCAGCGT GCCAGAAGTC AGGTGAAACC AGCAACAGCC 
AGCCAGCCCA TCCTCTCCAT GGTCGGACCC ACCAAGCTGA CGGTGGGGAA CTGGTCTCTG 
ACCTGCCTGA AGGAAGGCGA GATTGCAATT CACAACTCAG ACGGGCAGCA GGCCACGATC 
CTGAAGGAGG ATCTGCCCGG CTTCGTGTTC GAGTCCAACA GAGGAACCAA ACACTCGTTC 
ACTGCTGAAA CGTCGCTCGG TTCTGAGTTT GTCACTGGCT GGACGGGGAA ACGTGGCAGA 
AAGCTGAAGT CCAAGCTGGA GAAGACCAAG CAGAAGGTGA AGAGCATGGC CAGGGAGCTG 
TACGACGACC ACTTCAAAGC TGTGGAGAGC ATGCCCAGAG GAGTGGTGGT CACCCTGAGG 
AACATCGCCA CGCAGCTGGA GTCCGCCTGG GAGCTGCACA TAAACCGACA GTGTCTGGAA 
GGAGAGAACA CGTGGAGGGA CCTGATGAAG ACCGCTCTGG AGAACCTGAT TGTGGTTTTG 
AAGGATGAAA ACACGATTTC TCCGTATGAA ATGTGCAGCA GCGGCCTGGT TCAGGCTCTG 
TTTACCGTCC TCAGCAACAG TGTGGAGCTG GACCTGAAAC ATGACTGTAA GCCTCTAATG 
GAAAGGATCA ACGTCTTTAA AGCTGCTTTC AGCGAGAATG AAGACAATGA AAGCCGACCA 
GCCGTTGCCT TAGTGCGGAA GCTGATAGCC GTCCTGGAAT CGATCGAGCG TCTGCCCCTG 
CACCTGTATG ACACGCCTGG ATCCTCCTAC AATCTGCAGA TTCTGACGAG GAGGCTGCGG 
TTCCGTCTGG AGCGAGCGCC TGGGGAGACC GCTCTCATTG ACCGGACGGG ACGCATGCTG 
AAGATGGAGC CGCTCGCCAC CGTAGAGTCT CTGGAGCAGT ACCTGCTGAA AATGGTCGCT 
AAACAGTGGT ACGACTTTGA ACGCTCATCC TTCGTTTTTG TGAGAAAACT GAGAGAAGGA 
CAGATCTTCA CCTTCAGGCA CCAGCACGAC TTTGACGAGA ACGGCATCAT CTACTGGGTC 
GGAACCAACG CCAAGACGGC GTACGAGTGG GTGAACCCCG CAGCGTACGG CCTGGTGGTG 
GTGACCTCCT CCGAGGGGAG GAATCTGCCT TATGGGCGTT TGGAGGACAT CCTGAGTCGG 
GATAGCTCCG CCCTCAACTG CCACACGAAC GACGACAAGA ACGCCTGGTT CGCAGTTGAC 
CTCGGCCTCT GGGTGATTCC GTCAGCGTAC ACTCTGAGGC ACGCCAGGGG GTACGGACGC 
TCTGCGCTCA GGAACTGGGT TTTCCAAGTG TCCAAGGACG GACAGAACTG GTCCACACTC 
TACAACCACA TCGACGATGG CAGTCTCAAT GAACCGGGGT CTACGGCCAC GTGGCCTCTG 
GACCCGTCCA AAGAGGAGAA GCAGGGCTGG AGACACATCC GCATCAAGCA GATGGGGAAG 
AACGCCAGCG GTCAGACACA CTACCTGTCC TTATCTGGGC TGGAGCTGTA CGGCACGGTC 
ACCGCCGTAT GCGAGGACCA GCTGGGTAAA GCTGTGAAAG AGGCGGAGGC CAACCTTCGT 
CGCCAGCGGC GCCTCTTCCG TTCTCAGGTC ATGAAGTACA TCGTCCCAGG TGCGCGGGTC 
ATCCGTGGCA TCGACTGGAA GTGGAGGGAC CAGGATGGAA ACCCGGCAGG GGAAGGCACT 
GTCACTGGGG AGGCGCACAA CGGCTGGATT GATGTAACCT GGGATGCTGG CGGCTCTAAC 
TCTTATCGTA TGGGCGCCGA AGGGAAGTTT GACCTCAAGC TTGCTCCAGG GTACGACCCT 
GAGCTGGCTG CCACAGCGCC ATCACCCAAA CCTGTCTCAT CCGCTGTTTC AGGTCCCGCC 
TCCTCCACGG TGGGACCCTC TGCGACGCCA GCAGCTGTTG GCGGAACCAC CATCACCACC 
TCATCCTCCT CCACCACCTC ATCGTCCTCG CAGCAGCCTT CCTGGAGCAG TTTGGTGAAA 
AATAACTGTC CCGACAAGGG AGGGGCCAGC TCCTCCAGCA GGAAGGGTAG CAGCAGCTCC 
GTCTGCAGCG TCGCTTCCTC CTCTGACATC AGTCTGAGCT CCTCCATTGG GCTGCCTGGC 
GCTGGGAGCC TGCAGCTGGA CAGGAAGGCC GAGGGGCTGC TGCTAGATCA GGCCGCGGGG 
GTGGAAGGGG TGACGGGGGG AGGGACCGGT TCCGATGGAC ATCAGCAAGA GCCGATAGTC 
GTTCTGTCCT CCGTGACCGA ATCCGGTTCG GCGTCCAGCT CCGGCACGCT CACCGCCGAC 
GTGTCAACGG CGGGTGACGA GCATCACTCC CCCTCTGCAA TGGCCACTGC TGTGGACCCG 
GCCACGGCCA TCTCCATGGG CTTGGTGAGC GTCAGCTCCC CGGACGTCAG CTCCGTGTCG 
GAGCCCTCCA GCAAAGACAC TCACTCCCAG AGGCCGCTGT GCTCGGCCGT GAACACCCGG 
CTGTCCGTCA GCTCCCTGCT GGCCGCCAGC GCTCCCATGA GCTCCAGCGC CAGCGTGCCC 
AATCTGTCCT CTCGGGAGGC CAGCCTCATG GAGTCCTTCG TCCGCCGCGC GCCCAACATG 
TCCCGCACCA ACGCTACCAA CAACATGAAC CTGAGCCGCA GCAGCAGCGA CAACAACACC 
AACACACTGG GCAGGAACGT GATGAGCACA GCCAACTTCC TGGACAGTTG CCGGGCCAAC 
ACGCTGCTAG CAGAGCTGGA CGATGAGGAG GACCTCCCAG AGCCCGACGA CGACGACGAT 
GAGAATGAAG ACGACAATCA GGAGGATCAG GAGTATGAGG AGGTCCTGGA GGAGGAGGAG 
TATGAGACGA AGGGAGGACG GAGGAGGACA TGGGACGATG ACTTTGTCCT AAAAAGACAG 
TTTTCTGCTT TGGTCCCTGC CTTCGACCCC AGACCAGGAA GAACCAACGT CCAGCAGACC 
ACCGACCTGG AGATCCCCCC ACCAGGAACT CCGCGGTCGG AGGTTCGGGA GGAGGTGGAG 
TGTGCCCCAT CCCCCCACCT CGCCCTCACA CTGAAGGTGG CGGGTCTCGG CACGGCTCGG 
GAGGTGGAGA TCCCGCTCTC CAACTACAAG TTGACCATCT TCTACTATGT GCAGCGGCTC 
CTGCAGCTCT CCTGCAACGG CGCCGTAAAG ACGGACAAGC TGCGGCGCAT CTGGGAGCCG 
ACGTACACGA TCATGTACAG AGAGCTCAAA GACTCGGACA AAGAGAAGGA GAGCGGGAAG 
ATGGACCTCT TTGAGCACGT TGTTGGAAGT CGTTCTGGAG GTCTGAGCCC CAGCTCGGGT 
TCAGCCAATC AGAGCAGCGA GATCCTGGGC TGGGCGAAGG AGGCGGTCCT GGCCAAGGCG 
GGCTGCAGCC AGAACGCCTG CGGGGTGGAA GACGTCCTGC AGCTGCTGCG CATTCTCTAT 
GTGATTGGGA GAGACTCTAC TGCAGGCGGA CGCACGCTGC AAGAGGATGT TGACGAGCTG 
CAGTTCAACT CGCCTCCTGA GGAGTTCAGC AGCAAGAAGA TCACCACCAA GATCCTGCAG 
CAGATCGAGG AGCCCTTGGC TCTGGCTAGC GGAGCGCTGC CGGACTGGTG TGAGCAGCTC 
ACCTCCAAGT GTCCTTTCCT GATCCCCTTT GAGACCAGAC AGCTGTACTT CACCTGCACT 
GCCTTTGGGG CCTCCAGGGC GATCGTGTGG CTCCAGAACC GCCGCGAGGC GACCATGGAG 
CGCTCACGGC CATCCACCAC CGTCCGGCGA GACGACCCCG GAGAGTTCAG AGTCGGTCGG 
CTTAAACACG AGCGCGTTAA AGTTCCTCGA GGGGAGTCCA TGATGGAGTG GGCGGAGTCT 
GTGATGCAGA TCCACGCAAA CAGGAAGTCT GTTTTAGAGG TGGAGTTTCT GGGTGAGGAG 
GGGACGGGTC TGGGTCCGAC TCTGGAGTTC TACGCTCTGG TGGCGGCGGA GTTTCAGAGA 
ACGTCTCTGG GGATCTGGCT CTGTGACGAC GACTTCCCGG ACGACGAGTC CCGACAGGTG 
GATCTGGGTG GAGGTCTGAA GCCTCCTGGC TTCTACGTGC AGCGGTCCTG CGGGCTCTTC 
CCGGCGCCGT TCCCTCAGGA CAGCGAGGAG CTGGAGCGGA TTACGCGGCT CTTCCACTTC 
CTGGGGATCT TCCTGGCCAA GTGCATCCAG GACAACCGGC TGGTGGACCT GCCCGTGTCC 
CAGCCTTTCT TCAAGCTGCT CTGCATGGGG GACATCAAGT CCAACATGAG CAAGCTGCTG 
TACCAGTCCC GCGGCTCCCC CCAGGGCCAC CTCTCCGAGA GGGCCCCCCT GCTGCTGCTG 
ACGGAGGTGC AGTCGGAGGC GTCCACAGAG GAGAGCCAGG AGACCTACTC TGTGGGGAGC 
TTCGACGAGG ACTCCAAGTC AGACTTCATC ATGGACCCCC CCAAACCCAA GCCGCCCGCC 
TGGTTCCACG GCATCATGTC CTGGGACGAC TTCCATCTGG TCAACCCGCA CAGAGCCAGT 
TTCCTGAAGG AGGTGAAGGA GCTGGCAGTG AGGAGGAGAC AGATTCTGAC CAGCAAGAGT 
TTGTCAGAGG ATGAGAAGAA CACCAGGCTG CAGGACCTGA TGCTGAGGAA CCCGCTGGGC 
TCTGGGCCCC CCTTAAGCGT CGAAGACCTC GGGTTGAACT TCCAGTTCTG TCCGTCCTCC 
AAGATTCACG GCTTCTTCGC GGTGGATCTG AAACCAAACG GGGATGACGA GATGGTGAGT 
CTGGAGAACG CCGAGGAGTA CGTGGAGCTG ATGTTCGACT TCTGCATGCA CGCCGGCATC 
CAGAAGCAGA TGGAGGCGTT CAGAGAGGGT TTCAACCGCG TCTTCCCCAT GGAGAAGCTC 
AGCTCGTTCA GCCAGAAGGA GGTGCAGATG ATCCTCTGCG GAAACCAATC CCCCTGCTGG 
ACCGCCGATG ACATCATCAG CTACACCGAG CCAAAGCTGG GATACACCAG AGACAGCCCT 
GGGTTCCTGC GCTTCATCCG AGTTTTATGC GGAATGTCGA CGGACGAGAG GAAAGCTTTC 
CTGCAGTTCA CCACCGGCTG CTCCACGCTG CCCCCCGGTG GCCTGGCTAA CCTGCACCCG 
CGCCTCACCA TCGTCAGAAA GGTGGACGCC ACGGACTCCA GCTACCCATC GGTGAACACG 
TGCGTTCACT ACCTGAAGCT GCCGGAGTAC TCGTCAGAGG AGATCATGAG GGAGCGGCTG 
TTGGCGGCCA CCATGGAGAA GGGCTTCCAC CTCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hectd1 ( XM_020713930.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020569589.1 Oryzias latipes HECT domain E3 ubiquitin protein ligase 1 (hectd1), transcript variant X3, mRNA.

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CloneID	OJa76016
Clone ID Related Accession (Same CDS sequence)	XM_020713930.2 , XM_020713930.1
Accession Version	XM_020713930.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019880.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG TGGACCCAGA CACGTTGCTG GAGTGGCTGC AGATGGGACA GGGCGATGAG 
CGGGACATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGCTGCTCAT GTCGGACAAC 
GTGGACCGCT GCTTTGAGAC ATGTCCTCCT CGAACGTTCC TCCCAGCGCT TTGTAAGATC 
TTCTTGGACG AGAGCGCTCC TGATAACGTG CTGGAGGTCA CGGCCAGAGC CATCACCTAC 
TACCTGGACG TGTCGGCAGA ATGCACGCGG AGGATCGTGG GTGTGGACGG GGCCATAAAG 
GCCCTGTGCA ACCGGCTGGT GGTGGTGGAA CTCAACAACA GGACCAGCAG GGATCTGGCT 
GAGCAGTGTG TTAAGGTGCT GGAGCTGATC TGTACCAGGG AATCTGGCGC CGTGTTTGAA 
GCCAGCGGTC TGAACTGCGT TTTGAGCTTC ATACGCGACA GTGGTCACCT GGTCCACAAA 
GACACTCTGC ACTCTGCAAT GGCAGTGGTG TCTCGCCTCT GTAGCAAGAT GGAGCCGCAG 
GACTCTTCTC TAGAAACCTG CGTGGAGTCT CTGTCCAGCC TCCTGAAACA TGAGGATCAC 
CAGGTGTCAG ACGGTGCTCT GCGTTGCTTC GCCTCATTGG CTGATCGGTT TACTCGTCGA 
GGCGTAGACC CCGCCCCTCT GGCCAAACAT GGACTGACGG AGGAGCTGCT TTCCCGCATG 
GCGGCTGCCG GAGGTTCCGT GTCGGGCTCT TCTTCTGCCT GTAAACCAGG TCGGCCCTCC 
ACTGGTGCCA CACCGTCTGC CTCCGACTCC AAACTGAGCA ACCAGGTGTC CACCATCGTT 
AGCCTGCTGT CCACGCTCTG CCGGGGGTCG CCAGTGGTCA CTCATGACTT GTTACGTTCG 
GCACTCCCTA ACTCCATGGA GTCTGCGCTG GGAGGAGACG AGCGCTGTGT GCTGGACACC 
ATGCGGCTGG TCGACCTGCT GCTGGTGCTT CTGTTCGAGG GGCGGAAAGC GCTGCCAAAG 
TCCACCGCCG GCTCAGCAGG TCGGATCCCG GGGCTTCGCC GGCTGGACAG CTCAGGGGAG 
CGGTCGCACC GACAGCTCAT CGACTGCATC CGCAGCAAAG ACACGGACGC CCTGATAGAT 
GCCATCGACA CTGGAGCTTT TGAAGTGAAC TTCATGGACG ACGTGGGACA GACGCTGCTG 
AACTGGGCCT CAGCTTTTGG AACACAGGAA ATGGTGGAGT TTTTGTGCGA GAGGGGTGCA 
GATGTGAACC GAGGCCAGAG GTCATCCTCG CTGCACTACG CTGCCTGTTT CGGACGGCCA 
CAAGTTGCCA AGACGCTGCT GCGGCACGGA GCCAACCCGG ACCTGAGGGA TGAGGACGGG 
AAGACTCCTT TGGACAAAGC CAGGGAGAGG GGACACAGTG AGGTGGTGGC CATCCTTCAG 
TCTCCGGGAG ACTGGATGTG TCCAGTGAAC AAAGGAGACG ACAAGAGGAA GAAAGATGCG 
AGTAAGGAAG AAGAGGAGGG CAGTGAACCC AAAGGAGACC CAGAAATGGC TCCTTTCTAC 
CTGAAAAGAC TCCTCCCTGT TTTTGCACAA ACCTTTCAGC AAACCATGCT GCCTTCCATC 
AGGAAAGCCA GTCTGGCCCT CATCAGGAAG ATGGTTCACT ACAGCAGCGA GGTTCTGCTG 
AGGGAGGTGT GCGACAGTGA GACGGGACAC AACCTGCCCA CAGTGCTGGT GGAGATCACC 
GCCACCGTCC TCGACCAGGA GGATGATGAC GACGGTCACC TGCTGGCTCT GCAGATCATC 
AGGGATCTGG TGGACAAAGG TGGAGATGTT TTCCTTGACC AGCTGGCCCG ACTCGGGGTC 
ATCAACAAGG TGTCGACCTT GGCCGGACCG ACGTCTGATG ATGAGAACGA GGATGAAGTC 
AAACCTGAAA AGGAGGAGGA GGTGCAGGAA GATGCAAAGG AAATCCAGCA GGGGAAGCCG 
TACCACTGGA GGGACTGGTC CATCATCAGA GGCAGGGACT GCCTCTACAT TTGGTCAGAC 
GCCGCCGCCC TCGAGCTCTC CAACGGCTCC AATGGCTGGT TTCGCTTCAT CTTGGATGGG 
AAGCTCGCCA CCATGTACTC GAGTGGGAGC CCAGAGGGGG GGTCAGACAG CTCAGAGTCT 
CGCAGTGAGT TTTTGGAGAA GCTGCAGCGT GCCAGAAGTC AGGTGAAACC AGCAACAGCC 
AGCCAGCCCA TCCTCTCCAT GGTCGGACCC ACCAAGCTGA CGGTGGGGAA CTGGTCTCTG 
ACCTGCCTGA AGGAAGGCGA GATTGCAATT CACAACTCAG ACGGGCAGCA GGCCACGATC 
CTGAAGGAGG ATCTGCCCGG CTTCGTGTTC GAGTCCAACA GAGGAACCAA ACACTCGTTC 
ACTGCTGAAA CGTCGCTCGG TTCTGAGTTT GTCACTGGCT GGACGGGGAA ACGTGGCAGA 
AAGCTGAAGT CCAAGCTGGA GAAGACCAAG CAGAAGGTGA AGAGCATGGC CAGGGAGCTG 
TACGACGACC ACTTCAAAGC TGTGGAGAGC ATGCCCAGAG GAGTGGTGGT CACCCTGAGG 
AACATCGCCA CGCAGCTGGA GTCCGCCTGG GAGCTGCACA TAAACCGACA GTGTCTGGAA 
GGAGAGAACA CGTGGAGGGA CCTGATGAAG ACCGCTCTGG AGAACCTGAT TGTGGTTTTG 
AAGGATGAAA ACACGATTTC TCCGTATGAA ATGTGCAGCA GCGGCCTGGT TCAGGCTCTG 
TTTACCGTCC TCAGCAACAG TGTGGAGCTG GACCTGAAAC ATGACTGTAA GCCTCTAATG 
GAAAGGATCA ACGTCTTTAA AGCTGCTTTC AGCGAGAATG AAGACAATGA AAGCCGACCA 
GCCGTTGCCT TAGTGCGGAA GCTGATAGCC GTCCTGGAAT CGATCGAGCG TCTGCCCCTG 
CACCTGTATG ACACGCCTGG ATCCTCCTAC AATCTGCAGA TTCTGACGAG GAGGCTGCGG 
TTCCGTCTGG AGCGAGCGCC TGGGGAGACC GCTCTCATTG ACCGGACGGG ACGCATGCTG 
AAGATGGAGC CGCTCGCCAC CGTAGAGTCT CTGGAGCAGT ACCTGCTGAA AATGGTCGCT 
AAACAGTGGT ACGACTTTGA ACGCTCATCC TTCGTTTTTG TGAGAAAACT GAGAGAAGGA 
CAGATCTTCA CCTTCAGGCA CCAGCACGAC TTTGACGAGA ACGGCATCAT CTACTGGGTC 
GGAACCAACG CCAAGACGGC GTACGAGTGG GTGAACCCCG CAGCGTACGG CCTGGTGGTG 
GTGACCTCCT CCGAGGGGAG GAATCTGCCT TATGGGCGTT TGGAGGACAT CCTGAGTCGG 
GATAGCTCCG CCCTCAACTG CCACACGAAC GACGACAAGA ACGCCTGGTT CGCAGTTGAC 
CTCGGCCTCT GGGTGATTCC GTCAGCGTAC ACTCTGAGGC ACGCCAGGGG GTACGGACGC 
TCTGCGCTCA GGAACTGGGT TTTCCAAGTG TCCAAGGACG GACAGAACTG GTCCACACTC 
TACAACCACA TCGACGATGG CAGTCTCAAT GAACCGGGGT CTACGGCCAC GTGGCCTCTG 
GACCCGTCCA AAGAGGAGAA GCAGGGCTGG AGACACATCC GCATCAAGCA GATGGGGAAG 
AACGCCAGCG GTCAGACACA CTACCTGTCC TTATCTGGGC TGGAGCTGTA CGGCACGGTC 
ACCGCCGTAT GCGAGGACCA GCTGGGTAAA GCTGTGAAAG AGGCGGAGGC CAACCTTCGT 
CGCCAGCGGC GCCTCTTCCG TTCTCAGGTC ATGAAGTACA TCGTCCCAGG TGCGCGGGTC 
ATCCGTGGCA TCGACTGGAA GTGGAGGGAC CAGGATGGAA ACCCGGCAGG GGAAGGCACT 
GTCACTGGGG AGGCGCACAA CGGCTGGATT GATGTAACCT GGGATGCTGG CGGCTCTAAC 
TCTTATCGTA TGGGCGCCGA AGGGAAGTTT GACCTCAAGC TTGCTCCAGG GTACGACCCT 
GAGCTGGCTG CCACAGCGCC ATCACCCAAA CCTGTCTCAT CCGCTGTTTC AGGTCCCGCC 
TCCTCCACGG TGGGACCCTC TGCGACGCCA GCAGCTGTTG GCGGAACCAC CATCACCACC 
TCATCCTCCT CCACCACCTC ATCGTCCTCG CAGCAGCCTT CCTGGAGCAG TTTGGTGAAA 
AATAACTGTC CCGACAAGGG AGGGGCCAGC TCCTCCAGCA GGAAGGGTAG CAGCAGCTCC 
GTCTGCAGCG TCGCTTCCTC CTCTGACATC AGTCTGAGCT CCTCCATTGG GCTGCCTGGC 
GCTGGGAGCC TGCAGCTGGA CAGGAAGGCC GAGGGGCTGC TGCTAGATCA GGCCGCGGGG 
GTGGAAGGGG TGACGGGGGG AGGGACCGGT TCCGATGGAC ATCAGCAAGA GCCGATAGTC 
GTTCTGTCCT CCGTGACCGA ATCCGGTTCG GCGTCCAGCT CCGGCACGCT CACCGCCGAC 
GTGTCAACGG CGGGTGACGA GCATCACTCC CCCTCTGCAA TGGCCACTGC TGTGGACCCG 
GCCACGGCCA TCTCCATGGG CTTGGTGAGC GTCAGCTCCC CGGACGTCAG CTCCGTGTCG 
GAGCCCTCCA GCAAAGACAC TCACTCCCAG AGGCCGCTGT GCTCGGCCGT GAACACCCGG 
CTGTCCGTCA GCTCCCTGCT GGCCGCCAGC GCTCCCATGA GCTCCAGCGC CAGCGTGCCC 
AATCTGTCCT CTCGGGAGGC CAGCCTCATG GAGTCCTTCG TCCGCCGCGC GCCCAACATG 
TCCCGCACCA ACGCTACCAA CAACATGAAC CTGAGCCGCA GCAGCAGCGA CAACAACACC 
AACACACTGG GCAGGAACGT GATGAGCACA GCCAACTTCC TGGACAGTTG CCGGGCCAAC 
ACGCTGCTAG CAGAGCTGGA CGATGAGGAG GACCTCCCAG AGCCCGACGA CGACGACGAT 
GAGAATGAAG ACGACAATCA GGAGGATCAG GAGTATGAGG AGGTCCTGGA GGAGGAGGAG 
TATGAGACGA AGGGAGGACG GAGGAGGACA TGGGACGATG ACTTTGTCCT AAAAAGACAG 
TTTTCTGCTT TGGTCCCTGC CTTCGACCCC AGACCAGGAA GAACCAACGT CCAGCAGACC 
ACCGACCTGG AGATCCCCCC ACCAGGAACT CCGCGGTCGG AGGTTCGGGA GGAGGTGGAG 
TGTGCCCCAT CCCCCCACCT CGCCCTCACA CTGAAGGTGG CGGGTCTCGG CACGGCTCGG 
GAGGTGGAGA TCCCGCTCTC CAACTACAAG TTGACCATCT TCTACTATGT GCAGCGGCTC 
CTGCAGCTCT CCTGCAACGG CGCCGTAAAG ACGGACAAGC TGCGGCGCAT CTGGGAGCCG 
ACGTACACGA TCATGTACAG AGAGCTCAAA GACTCGGACA AAGAGAAGGA GAGCGGGAAG 
ATGGACCTCT TTGAGCACGT TGTTGGAAGT CGTTCTGGAG GTCTGAGCCC CAGCTCGGGT 
TCAGCCAATC AGAGCAGCGA GATCCTGGGC TGGGCGAAGG AGGCGGTCCT GGCCAAGGCG 
GGCTGCAGCC AGAACGCCTG CGGGGTGGAA GACGTCCTGC AGCTGCTGCG CATTCTCTAT 
GTGATTGGGA GAGACTCTAC TGCAGGCGGA CGCACGCTGC AAGAGGATGT TGACGAGCTG 
CAGTTCAACT CGCCTCCTGA GGAGTTCAGC AGCAAGAAGA TCACCACCAA GATCCTGCAG 
CAGATCGAGG AGCCCTTGGC TCTGGCTAGC GGAGCGCTGC CGGACTGGTG TGAGCAGCTC 
ACCTCCAAGT GTCCTTTCCT GATCCCCTTT GAGACCAGAC AGCTGTACTT CACCTGCACT 
GCCTTTGGGG CCTCCAGGGC GATCGTGTGG CTCCAGAACC GCCGCGAGGC GACCATGGAG 
CGCTCACGGC CATCCACCAC CGTCCGGCGA GACGACCCCG GAGAGTTCAG AGTCGGTCGG 
CTTAAACACG AGCGCGTTAA AGTTCCTCGA GGGGAGTCCA TGATGGAGTG GGCGGAGTCT 
GTGATGCAGA TCCACGCAAA CAGGAAGTCT GTTTTAGAGG TGGAGTTTCT GGGTGAGGAG 
GGGACGGGTC TGGGTCCGAC TCTGGAGTTC TACGCTCTGG TGGCGGCGGA GTTTCAGAGA 
ACGTCTCTGG GGATCTGGCT CTGTGACGAC GACTTCCCGG ACGACGAGTC CCGACAGGTG 
GATCTGGGTG GAGGTCTGAA GCCTCCTGGC TTCTACGTGC AGCGGTCCTG CGGGCTCTTC 
CCGGCGCCGT TCCCTCAGGA CAGCGAGGAG CTGGAGCGGA TTACGCGGCT CTTCCACTTC 
CTGGGGATCT TCCTGGCCAA GTGCATCCAG GACAACCGGC TGGTGGACCT GCCCGTGTCC 
CAGCCTTTCT TCAAGCTGCT CTGCATGGGG GACATCAAGT CCAACATGAG CAAGCTGCTG 
TACCAGTCCC GCGGCTCCCC CCAGGGCCAC CTCTCCGAGA GGGCCCCCCT GCTGCTGCTG 
ACGGAGGTGC AGTCGGAGGC GTCCACAGAG GAGAGCCAGG AGACCTACTC TGTGGGGAGC 
TTCGACGAGG ACTCCAAGTC AGACTTCATC ATGGACCCCC CCAAACCCAA GCCGCCCGCC 
TGGTTCCACG GCATCATGTC CTGGGACGAC TTCCATCTGG TCAACCCGCA CAGAGCCAGT 
TTCCTGAAGG AGGTGAAGGA GCTGGCAGTG AGGAGGAGAC AGATTCTGAC CAGCAAGAGT 
TTGTCAGAGG ATGAGAAGAA CACCAGGCTG CAGGACCTGA TGCTGAGGAA CCCGCTGGGC 
TCTGGGCCCC CCTTAAGCGT CGAAGACCTC GGGTTGAACT TCCAGTTCTG TCCGTCCTCC 
AAGATTCACG GCTTCTTCGC GGTGGATCTG AAACCAAACG GGGATGACGA GATGGTGAGT 
CTGGAGAACG CCGAGGAGTA CGTGGAGCTG ATGTTCGACT TCTGCATGCA CGCCGGCATC 
CAGAAGCAGA TGGAGGCGTT CAGAGAGGGT TTCAACCGCG TCTTCCCCAT GGAGAAGCTC 
AGCTCGTTCA GCCAGAAGGA GGTGCAGATG ATCCTCTGCG GAAACCAATC CCCCTGCTGG 
ACCGCCGATG ACATCATCAG CTACACCGAG CCAAAGCTGG GATACACCAG AGACAGCCCT 
GGGTTCCTGC GCTTCATCCG AGTTTTATGC GGAATGTCGA CGGACGAGAG GAAAGCTTTC 
CTGCAGTTCA CCACCGGCTG CTCCACGCTG CCCCCCGGTG GCCTGGCTAA CCTGCACCCG 
CGCCTCACCA TCGTCAGAAA GGTGGACGCC ACGGACTCCA GCTACCCATC GGTGAACACG 
TGCGTTCACT ACCTGAAGCT GCCGGAGTAC TCGTCAGAGG AGATCATGAG GGAGCGGCTG 
TTGGCGGCCA CCATGGAGAA GGGCTTCCAC CTCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020569589.1
CDS	361..7839
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEASGLNCVLSFIRDSGHLVHKDTLHSAMAVVSRLCSKMEPQDSSLETCVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGSVSGSSSACKPGRPSTGATPSASDSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSALPNSMESALGGDERCVLDTMRLVDLLLVLLFEGRKALPKSTAGSAGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKRKKDASKEEEEGSEPKGDPEMAPFYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMVHYSSEVLLREVCDSETGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDVFLDQLARLGVINKVSTLAGPTSDDENEDEVKPEKEEEVQEDAKEIQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARSQVKPATASQPILSMVGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVKSMARELYDDHFKAVESMPRGVVVTLRNIATQLESAWELHINRQCLEGENTWRDLMKTALENLIVVLKDENTISPYEMCSSGLVQALFTVLSNSVELDLKHDCKPLMERINVFKAAFSENEDNESRPAVALVRKLIAVLESIERLPLHLYDTPGSSYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFERSSFVFVRKLREGQIFTFRHQHDFDENGIIYWVGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDSSALNCHTNDDKNAWFAVDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWSTLYNHIDDGSLNEPGSTATWPLDPSKEEKQGWRHIRIKQMGKNASGQTHYLSLSGLELYGTVTAVCEDQLGKAVKEAEANLRRQRRLFRSQVMKYIVPGARVIRGIDWKWRDQDGNPAGEGTVTGEAHNGWIDVTWDAGGSNSYRMGAEGKFDLKLAPGYDPELAATAPSPKPVSSAVSGPASSTVGPSATPAAVGGTTITTSSSSTTSSSSQQPSWSSLVKNNCPDKGGASSSSRKGSSSSVCSVASSSDISLSSSIGLPGAGSLQLDRKAEGLLLDQAAGVEGVTGGGTGSDGHQQEPIVVLSSVTESGSASSSGTLTADVSTAGDEHHSPSAMATAVDPATAISMGLVSVSSPDVSSVSEPSSKDTHSQRPLCSAVNTRLSVSSLLAASAPMSSSASVPNLSSREASLMESFVRRAPNMSRTNATNNMNLSRSSSDNNTNTLGRNVMSTANFLDSCRANTLLAELDDEEDLPEPDDDDDENEDDNQEDQEYEEVLEEEEYETKGGRRRTWDDDFVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPRSEVREEVECAPSPHLALTLKVAGLGTAREVEIPLSNYKLTIFYYVQRLLQLSCNGAVKTDKLRRIWEPTYTIMYRELKDSDKEKESGKMDLFEHVVGSRSGGLSPSSGSANQSSEILGWAKEAVLAKAGCSQNACGVEDVLQLLRILYVIGRDSTAGGRTLQEDVDELQFNSPPEEFSSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATMERSRPSTTVRRDDPGEFRVGRLKHERVKVPRGESMMEWAESVMQIHANRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTSLGIWLCDDDFPDDESRQVDLGGGLKPPGFYVQRSCGLFPAPFPQDSEELERITRLFHFLGIFLAKCIQDNRLVDLPVSQPFFKLLCMGDIKSNMSKLLYQSRGSPQGHLSERAPLLLLTEVQSEASTEESQETYSVGSFDEDSKSDFIMDPPKPKPPAWFHGIMSWDDFHLVNPHRASFLKEVKELAVRRRQILTSKSLSEDEKNTRLQDLMLRNPLGSGPPLSVEDLGLNFQFCPSSKIHGFFAVDLKPNGDDEMVSLENAEEYVELMFDFCMHAGIQKQMEAFREGFNRVFPMEKLSSFSQKEVQMILCGNQSPCWTADDIISYTEPKLGYTRDSPGFLRFIRVLCGMSTDERKAFLQFTTGCSTLPPGGLANLHPRLTIVRKVDATDSSYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_020713930.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes HECT domain E3 ubiquitin protein ligase 1 (hectd1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020713930.1

ORF Insert Sequence:

ATGGCGGATG TGGACCCAGA CACGTTGCTG GAGTGGCTGC AGATGGGACA GGGCGATGAG 
CGGGACATGC AGCTCATCGC CTTGGAGCAG CTCTGCATGC TGCTGCTCAT GTCGGACAAC 
GTGGACCGCT GCTTTGAGAC ATGTCCTCCT CGAACGTTCC TCCCAGCGCT TTGTAAGATC 
TTCTTGGACG AGAGCGCTCC TGATAACGTG CTGGAGGTCA CGGCCAGAGC CATCACCTAC 
TACCTGGACG TGTCGGCAGA ATGCACGCGG AGGATCGTGG GTGTGGACGG GGCCATAAAG 
GCCCTGTGCA ACCGGCTGGT GGTGGTGGAA CTCAACAACA GGACCAGCAG GGATCTGGCT 
GAGCAGTGTG TTAAGGTGCT GGAGCTGATC TGTACCAGGG AATCTGGCGC CGTGTTTGAA 
GCCAGCGGTC TGAACTGCGT TTTGAGCTTC ATACGCGACA GTGGTCACCT GGTCCACAAA 
GACACTCTGC ACTCTGCAAT GGCAGTGGTG TCTCGCCTCT GTAGCAAGAT GGAGCCGCAG 
GACTCTTCTC TAGAAACCTG CGTGGAGTCT CTGTCCAGCC TCCTGAAACA TGAGGATCAC 
CAGGTGTCAG ACGGTGCTCT GCGTTGCTTC GCCTCATTGG CTGATCGGTT TACTCGTCGA 
GGCGTAGACC CCGCCCCTCT GGCCAAACAT GGACTGACGG AGGAGCTGCT TTCCCGCATG 
GCGGCTGCCG GAGGTTCCGT GTCGGGCTCT TCTTCTGCCT GTAAACCAGG TCGGCCCTCC 
ACTGGTGCCA CACCGTCTGC CTCCGACTCC AAACTGAGCA ACCAGGTGTC CACCATCGTT 
AGCCTGCTGT CCACGCTCTG CCGGGGGTCG CCAGTGGTCA CTCATGACTT GTTACGTTCG 
GCACTCCCTA ACTCCATGGA GTCTGCGCTG GGAGGAGACG AGCGCTGTGT GCTGGACACC 
ATGCGGCTGG TCGACCTGCT GCTGGTGCTT CTGTTCGAGG GGCGGAAAGC GCTGCCAAAG 
TCCACCGCCG GCTCAGCAGG TCGGATCCCG GGGCTTCGCC GGCTGGACAG CTCAGGGGAG 
CGGTCGCACC GACAGCTCAT CGACTGCATC CGCAGCAAAG ACACGGACGC CCTGATAGAT 
GCCATCGACA CTGGAGCTTT TGAAGTGAAC TTCATGGACG ACGTGGGACA GACGCTGCTG 
AACTGGGCCT CAGCTTTTGG AACACAGGAA ATGGTGGAGT TTTTGTGCGA GAGGGGTGCA 
GATGTGAACC GAGGCCAGAG GTCATCCTCG CTGCACTACG CTGCCTGTTT CGGACGGCCA 
CAAGTTGCCA AGACGCTGCT GCGGCACGGA GCCAACCCGG ACCTGAGGGA TGAGGACGGG 
AAGACTCCTT TGGACAAAGC CAGGGAGAGG GGACACAGTG AGGTGGTGGC CATCCTTCAG 
TCTCCGGGAG ACTGGATGTG TCCAGTGAAC AAAGGAGACG ACAAGAGGAA GAAAGATGCG 
AGTAAGGAAG AAGAGGAGGG CAGTGAACCC AAAGGAGACC CAGAAATGGC TCCTTTCTAC 
CTGAAAAGAC TCCTCCCTGT TTTTGCACAA ACCTTTCAGC AAACCATGCT GCCTTCCATC 
AGGAAAGCCA GTCTGGCCCT CATCAGGAAG ATGGTTCACT ACAGCAGCGA GGTTCTGCTG 
AGGGAGGTGT GCGACAGTGA GACGGGACAC AACCTGCCCA CAGTGCTGGT GGAGATCACC 
GCCACCGTCC TCGACCAGGA GGATGATGAC GACGGTCACC TGCTGGCTCT GCAGATCATC 
AGGGATCTGG TGGACAAAGG TGGAGATGTT TTCCTTGACC AGCTGGCCCG ACTCGGGGTC 
ATCAACAAGG TGTCGACCTT GGCCGGACCG ACGTCTGATG ATGAGAACGA GGATGAAGTC 
AAACCTGAAA AGGAGGAGGA GGTGCAGGAA GATGCAAAGG AAATCCAGCA GGGGAAGCCG 
TACCACTGGA GGGACTGGTC CATCATCAGA GGCAGGGACT GCCTCTACAT TTGGTCAGAC 
GCCGCCGCCC TCGAGCTCTC CAACGGCTCC AATGGCTGGT TTCGCTTCAT CTTGGATGGG 
AAGCTCGCCA CCATGTACTC GAGTGGGAGC CCAGAGGGGG GGTCAGACAG CTCAGAGTCT 
CGCAGTGAGT TTTTGGAGAA GCTGCAGCGT GCCAGAAGTC AGGTGAAACC AGCAACAGCC 
AGCCAGCCCA TCCTCTCCAT GGTCGGACCC ACCAAGCTGA CGGTGGGGAA CTGGTCTCTG 
ACCTGCCTGA AGGAAGGCGA GATTGCAATT CACAACTCAG ACGGGCAGCA GGCCACGATC 
CTGAAGGAGG ATCTGCCCGG CTTCGTGTTC GAGTCCAACA GAGGAACCAA ACACTCGTTC 
ACTGCTGAAA CGTCGCTCGG TTCTGAGTTT GTCACTGGCT GGACGGGGAA ACGTGGCAGA 
AAGCTGAAGT CCAAGCTGGA GAAGACCAAG CAGAAGGTGA AGAGCATGGC CAGGGAGCTG 
TACGACGACC ACTTCAAAGC TGTGGAGAGC ATGCCCAGAG GAGTGGTGGT CACCCTGAGG 
AACATCGCCA CGCAGCTGGA GTCCGCCTGG GAGCTGCACA TAAACCGACA GTGTCTGGAA 
GGAGAGAACA CGTGGAGGGA CCTGATGAAG ACCGCTCTGG AGAACCTGAT TGTGGTTTTG 
AAGGATGAAA ACACGATTTC TCCGTATGAA ATGTGCAGCA GCGGCCTGGT TCAGGCTCTG 
TTTACCGTCC TCAGCAACAG TGTGGAGCTG GACCTGAAAC ATGACTGTAA GCCTCTAATG 
GAAAGGATCA ACGTCTTTAA AGCTGCTTTC AGCGAGAATG AAGACAATGA AAGCCGACCA 
GCCGTTGCCT TAGTGCGGAA GCTGATAGCC GTCCTGGAAT CGATCGAGCG TCTGCCCCTG 
CACCTGTATG ACACGCCTGG ATCCTCCTAC AATCTGCAGA TTCTGACGAG GAGGCTGCGG 
TTCCGTCTGG AGCGAGCGCC TGGGGAGACC GCTCTCATTG ACCGGACGGG ACGCATGCTG 
AAGATGGAGC CGCTCGCCAC CGTAGAGTCT CTGGAGCAGT ACCTGCTGAA AATGGTCGCT 
AAACAGTGGT ACGACTTTGA ACGCTCATCC TTCGTTTTTG TGAGAAAACT GAGAGAAGGA 
CAGATCTTCA CCTTCAGGCA CCAGCACGAC TTTGACGAGA ACGGCATCAT CTACTGGGTC 
GGAACCAACG CCAAGACGGC GTACGAGTGG GTGAACCCCG CAGCGTACGG CCTGGTGGTG 
GTGACCTCCT CCGAGGGGAG GAATCTGCCT TATGGGCGTT TGGAGGACAT CCTGAGTCGG 
GATAGCTCCG CCCTCAACTG CCACACGAAC GACGACAAGA ACGCCTGGTT CGCAGTTGAC 
CTCGGCCTCT GGGTGATTCC GTCAGCGTAC ACTCTGAGGC ACGCCAGGGG GTACGGACGC 
TCTGCGCTCA GGAACTGGGT TTTCCAAGTG TCCAAGGACG GACAGAACTG GTCCACACTC 
TACAACCACA TCGACGATGG CAGTCTCAAT GAACCGGGGT CTACGGCCAC GTGGCCTCTG 
GACCCGTCCA AAGAGGAGAA GCAGGGCTGG AGACACATCC GCATCAAGCA GATGGGGAAG 
AACGCCAGCG GTCAGACACA CTACCTGTCC TTATCTGGGC TGGAGCTGTA CGGCACGGTC 
ACCGCCGTAT GCGAGGACCA GCTGGGTAAA GCTGTGAAAG AGGCGGAGGC CAACCTTCGT 
CGCCAGCGGC GCCTCTTCCG TTCTCAGGTC ATGAAGTACA TCGTCCCAGG TGCGCGGGTC 
ATCCGTGGCA TCGACTGGAA GTGGAGGGAC CAGGATGGAA ACCCGGCAGG GGAAGGCACT 
GTCACTGGGG AGGCGCACAA CGGCTGGATT GATGTAACCT GGGATGCTGG CGGCTCTAAC 
TCTTATCGTA TGGGCGCCGA AGGGAAGTTT GACCTCAAGC TTGCTCCAGG GTACGACCCT 
GAGCTGGCTG CCACAGCGCC ATCACCCAAA CCTGTCTCAT CCGCTGTTTC AGGTCCCGCC 
TCCTCCACGG TGGGACCCTC TGCGACGCCA GCAGCTGTTG GCGGAACCAC CATCACCACC 
TCATCCTCCT CCACCACCTC ATCGTCCTCG CAGCAGCCTT CCTGGAGCAG TTTGGTGAAA 
AATAACTGTC CCGACAAGGG AGGGGCCAGC TCCTCCAGCA GGAAGGGTAG CAGCAGCTCC 
GTCTGCAGCG TCGCTTCCTC CTCTGACATC AGTCTGAGCT CCTCCATTGG GCTGCCTGGC 
GCTGGGAGCC TGCAGCTGGA CAGGAAGGCC GAGGGGCTGC TGCTAGATCA GGCCGCGGGG 
GTGGAAGGGG TGACGGGGGG AGGGACCGGT TCCGATGGAC ATCAGCAAGA GCCGATAGTC 
GTTCTGTCCT CCGTGACCGA ATCCGGTTCG GCGTCCAGCT CCGGCACGCT CACCGCCGAC 
GTGTCAACGG CGGGTGACGA GCATCACTCC CCCTCTGCAA TGGCCACTGC TGTGGACCCG 
GCCACGGCCA TCTCCATGGG CTTGGTGAGC GTCAGCTCCC CGGACGTCAG CTCCGTGTCG 
GAGCCCTCCA GCAAAGACAC TCACTCCCAG AGGCCGCTGT GCTCGGCCGT GAACACCCGG 
CTGTCCGTCA GCTCCCTGCT GGCCGCCAGC GCTCCCATGA GCTCCAGCGC CAGCGTGCCC 
AATCTGTCCT CTCGGGAGGC CAGCCTCATG GAGTCCTTCG TCCGCCGCGC GCCCAACATG 
TCCCGCACCA ACGCTACCAA CAACATGAAC CTGAGCCGCA GCAGCAGCGA CAACAACACC 
AACACACTGG GCAGGAACGT GATGAGCACA GCCAACTTCC TGGACAGTTG CCGGGCCAAC 
ACGCTGCTAG CAGAGCTGGA CGATGAGGAG GACCTCCCAG AGCCCGACGA CGACGACGAT 
GAGAATGAAG ACGACAATCA GGAGGATCAG GAGTATGAGG AGGTCCTGGA GGAGGAGGAG 
TATGAGACGA AGGGAGGACG GAGGAGGACA TGGGACGATG ACTTTGTCCT AAAAAGACAG 
TTTTCTGCTT TGGTCCCTGC CTTCGACCCC AGACCAGGAA GAACCAACGT CCAGCAGACC 
ACCGACCTGG AGATCCCCCC ACCAGGAACT CCGCGGTCGG AGGTTCGGGA GGAGGTGGAG 
TGTGCCCCAT CCCCCCACCT CGCCCTCACA CTGAAGGTGG CGGGTCTCGG CACGGCTCGG 
GAGGTGGAGA TCCCGCTCTC CAACTACAAG TTGACCATCT TCTACTATGT GCAGCGGCTC 
CTGCAGCTCT CCTGCAACGG CGCCGTAAAG ACGGACAAGC TGCGGCGCAT CTGGGAGCCG 
ACGTACACGA TCATGTACAG AGAGCTCAAA GACTCGGACA AAGAGAAGGA GAGCGGGAAG 
ATGGACCTCT TTGAGCACGT TGTTGGAAGT CGTTCTGGAG GTCTGAGCCC CAGCTCGGGT 
TCAGCCAATC AGAGCAGCGA GATCCTGGGC TGGGCGAAGG AGGCGGTCCT GGCCAAGGCG 
GGCTGCAGCC AGAACGCCTG CGGGGTGGAA GACGTCCTGC AGCTGCTGCG CATTCTCTAT 
GTGATTGGGA GAGACTCTAC TGCAGGCGGA CGCACGCTGC AAGAGGATGT TGACGAGCTG 
CAGTTCAACT CGCCTCCTGA GGAGTTCAGC AGCAAGAAGA TCACCACCAA GATCCTGCAG 
CAGATCGAGG AGCCCTTGGC TCTGGCTAGC GGAGCGCTGC CGGACTGGTG TGAGCAGCTC 
ACCTCCAAGT GTCCTTTCCT GATCCCCTTT GAGACCAGAC AGCTGTACTT CACCTGCACT 
GCCTTTGGGG CCTCCAGGGC GATCGTGTGG CTCCAGAACC GCCGCGAGGC GACCATGGAG 
CGCTCACGGC CATCCACCAC CGTCCGGCGA GACGACCCCG GAGAGTTCAG AGTCGGTCGG 
CTTAAACACG AGCGCGTTAA AGTTCCTCGA GGGGAGTCCA TGATGGAGTG GGCGGAGTCT 
GTGATGCAGA TCCACGCAAA CAGGAAGTCT GTTTTAGAGG TGGAGTTTCT GGGTGAGGAG 
GGGACGGGTC TGGGTCCGAC TCTGGAGTTC TACGCTCTGG TGGCGGCGGA GTTTCAGAGA 
ACGTCTCTGG GGATCTGGCT CTGTGACGAC GACTTCCCGG ACGACGAGTC CCGACAGGTG 
GATCTGGGTG GAGGTCTGAA GCCTCCTGGC TTCTACGTGC AGCGGTCCTG CGGGCTCTTC 
CCGGCGCCGT TCCCTCAGGA CAGCGAGGAG CTGGAGCGGA TTACGCGGCT CTTCCACTTC 
CTGGGGATCT TCCTGGCCAA GTGCATCCAG GACAACCGGC TGGTGGACCT GCCCGTGTCC 
CAGCCTTTCT TCAAGCTGCT CTGCATGGGG GACATCAAGT CCAACATGAG CAAGCTGCTG 
TACCAGTCCC GCGGCTCCCC CCAGGGCCAC CTCTCCGAGA GGGCCCCCCT GCTGCTGCTG 
ACGGAGGTGC AGTCGGAGGC GTCCACAGAG GAGAGCCAGG AGACCTACTC TGTGGGGAGC 
TTCGACGAGG ACTCCAAGTC AGACTTCATC ATGGACCCCC CCAAACCCAA GCCGCCCGCC 
TGGTTCCACG GCATCATGTC CTGGGACGAC TTCCATCTGG TCAACCCGCA CAGAGCCAGT 
TTCCTGAAGG AGGTGAAGGA GCTGGCAGTG AGGAGGAGAC AGATTCTGAC CAGCAAGAGT 
TTGTCAGAGG ATGAGAAGAA CACCAGGCTG CAGGACCTGA TGCTGAGGAA CCCGCTGGGC 
TCTGGGCCCC CCTTAAGCGT CGAAGACCTC GGGTTGAACT TCCAGTTCTG TCCGTCCTCC 
AAGATTCACG GCTTCTTCGC GGTGGATCTG AAACCAAACG GGGATGACGA GATGGTGAGT 
CTGGAGAACG CCGAGGAGTA CGTGGAGCTG ATGTTCGACT TCTGCATGCA CGCCGGCATC 
CAGAAGCAGA TGGAGGCGTT CAGAGAGGGT TTCAACCGCG TCTTCCCCAT GGAGAAGCTC 
AGCTCGTTCA GCCAGAAGGA GGTGCAGATG ATCCTCTGCG GAAACCAATC CCCCTGCTGG 
ACCGCCGATG ACATCATCAG CTACACCGAG CCAAAGCTGG GATACACCAG AGACAGCCCT 
GGGTTCCTGC GCTTCATCCG AGTTTTATGC GGAATGTCGA CGGACGAGAG GAAAGCTTTC 
CTGCAGTTCA CCACCGGCTG CTCCACGCTG CCCCCCGGTG GCCTGGCTAA CCTGCACCCG 
CGCCTCACCA TCGTCAGAAA GGTGGACGCC ACGGACTCCA GCTACCCATC GGTGAACACG 
TGCGTTCACT ACCTGAAGCT GCCGGAGTAC TCGTCAGAGG AGATCATGAG GGAGCGGCTG 
TTGGCGGCCA CCATGGAGAA GGGCTTCCAC CTCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PHONE

1-877-436-7274

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hectd1 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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PHONE

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