LOC101166941 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101166941
Entrez Gene ID	101166941
Full Name	kalirin-like
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020707971.1	XP_020563630.1	kalirin-like isoform X1
XM_023964367.1	XP_023820135.1	kalirin isoform X2
XM_020707977.1	XP_020563636.1	kalirin-like isoform X3
XM_011482977.2	XP_011481279.2	kalirin-like isoform X2
XM_011482977.3	XP_011481279.2	kalirin isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC101166941 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101166941 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa112272	XM_023964367.1 Latest version!	Oryzias latipes kalirin (LOC101166941), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OJa74578	XM_020707971.1 Latest version!	Oryzias latipes kalirin-like (LOC101166941), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OJa74579	XM_011482977.3 Latest version!	Oryzias latipes kalirin (LOC101166941), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OJa74580	XM_020707977.1 Latest version!	Oryzias latipes kalirin-like (LOC101166941), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
OJa74579	XM_011482977.2	Oryzias latipes kalirin-like (LOC101166941), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101166941 ( XM_023964367.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023820135.1 Oryzias latipes kalirin (LOC101166941), transcript variant X2, mRNA.

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CloneID	OJa112272
Clone ID Related Accession (Same CDS sequence)	XM_023964367.1
Accession Version	XM_023964367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	kalirin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCGG CAGATGGAGG AGGAGAAGCG GGCCCGGACG GCCAGGCGAC GGCAGGTTCT 
GGTAAAGGGG AGTCTGTCCG GGCGGTCGAT GTCTTGACGG TGCTCAGAGA AAAAGTGGCC 
TTTGTGTCGG GTGGACGCGA CAAACGAGGT GGACCAATTC TCACCTTTCC TGCTAGGACC 
AATCACGATC GAATAAAACA GGAAGACCTC AGCCGCTTGG TTACTTATTT ATCAACTATC 
CCTAGCGAGG ATGTGCGTGC CCGTGGCTTT ACCGTTGTGG TGGATATGCG GGGCAGTAAG 
TGGGACAGCG TGAAGCCGGT TCTGCGAACA CTACAGGAGT CCTTTTCCTC AAACATCCAC 
ACAGCACTAC TCATCAAACC GGACACCTTC TGGCAGAAAC ACAAGACCAA CCTAGGCAGT 
GCCAAGCTGA GTTTCGAGAC CAGTCTGGTG TCAGTGGAGG GTTTGTCCCG ACTTATTGAT 
CCCTCTCAGC TGACCGCTGA CCTCGGAGGT TCATTGGAGT ATGACCATGT GGAGTGGACT 
GAGCTGCGTC TTGGTTTAGA GGAGTTCTCA GGGGCTGCCA CACAGCTACT AACCCAACTG 
GAGCAGCTGG AAGCCGGGCT GAGCCGCATA CCCGAGCCTC AGGACATTGA TGAGGCCCGC 
AAACTCTTGG AAGAACACAC TCGCCTCCGC AATAGCATCG CCACTGCGCC TGTTGATGCC 
CTCGACCGAG AAGGGAAGTG TTTACTTCAG CGGATGCGCC TAGGTTCATC CCACGGTGAA 
GCCTCAAATG AGAGTGGGGG TGGTGGGGGA ACTTGGTTGT CAGGAGGAGC AGATTTCCAG 
AGCGTGGCGC CGAAGGTTTC GTCTTTATTG GACCGGCTGC AGGGGGCTCG CAGTCACTTA 
CTGCAGTGCT GGGGTGATAA GAAGCTGCAC TTGGACCAGG CACTGCAACT CCACCTGTTT 
GAACAGGATG CTACCAAGAT GTCAGAGTGG ATCGCTCACA GTAAAGAGCT CTTCATGCAG 
AGTCTGGCAG AAGTCGGTCA GAACCACCAG CACGCTCTCG ATCTACAAAC GCAGCACCAG 
CATTTCACCG CCAACTGCAT GAACGGCAGT GTGAATGTGG ACAGTGTCGT TACCGTGGCA 
GCAAGGCTCG CGGAGGCCGG CCACTACGGA GCAGAGCGGA TTGCTTTGGT GTCGTCAAAG 
TTACAGGACA ACTGGAAGTC AATGACAAGT GCTCTGGAAG AGCGGAGCAA CATGCTTGCC 
ATGGCAACCG GTTTCCATCA AGGGGCAGAG CAGTTTCTAG CCCGAGTGGA GGGATGGGTT 
AAACTGTGCT CTGAAGGGCC ATTGCCATCA ACAACAGCCG AGCTGGAACC TGCAATAAAA 
AAACACCAGG AGCTCAATGA GGAGATTTCA GCCAACTACA CACAGGTCAG TGAAAGCGGT 
AAGGCATTGC TGGAGGTCCT TCAGCGTTGC CCCACAACAG ATTCTGACGA ATCAGCAACC 
AAACCAGATT TCACTGCGGC CACACACACC ATTATGGGGG TTCTACACCA GGTGATGCAG 
GGTCATCACG AAGTAGAGGG AGCATGGCAG CATCGTAAGC TCCGCCTCCA CCAGCGCCTG 
CAGTTATGTG TGTTTCAACA GGATGTTAAA CAGGTTTTGG ACTGGGTGGA GCAGCATGGA 
GAGGTTTTCC TGAACAAACA CACGGGTGTG GGGAAGTCCT TGCACCGGGC ACGGGCGCTG 
CAGAAGCGAC ACGATGACTT TCAGCAGGTT GCACAGAATA CGTACACCAA TGCAGAGAAG 
CTTCTGGAGG CAGCAGACCA GTTGGCGCAG TCTGGAGAGT GTGAGGAAGA GGAGATCTAC 
CAGGCAGCCA GAGACCTGGA GCTGCGAATG CAGGCCTTCA TTCAGCGCGT TGAACAGAGG 
AAGCTGCTGC TGGACCTCGC CGTATCCTTC TACACGCACA CAAAGGAGCT CTCTGCATGG 
ATGGAGGGCC TCCAGAAGCA GCTCTCCTCA GACCTCCTCG TGTGCCCGGA CACTGTGGAA 
GCTCTGCAGG CTTTAATCAG CCAACAGCAG CAGCAGCAGA CCAACACTCA GGAAGCTGCG 
ATGAGTGTCA TCCGGGAAGG AGAAGACCTC ATCCTGCAAC TCAGGCCTCA GGGCAGCTCG 
GTGGCCCATG TGGAATCGGT TTTGCAGCAA CTGGAAGAGT CTCATGTTCA GCTTGAGGAA 
CTTTTTCACC AGAGGAAGAT CCGAGTGGAC ATTTTCCTGC AGTTCCGCAT ACTGCACCAG 
TGCACGCTGG AGGTTACAGC TGAGATGGAT GCTTGGAAAA TGGACTTGGA AAAACAATCC 
CAGGAATTTT CCTCGGAGAA GGTCGCCTCA AATCGGCTTG CTGAAACCAA CCAGGACAAG 
CTGACCCAGG ATAAGCTGGC ACTAGGACAG TCCCGGCTTG TGGAGTCCAG CCCGGAAGCG 
TTAACACTAG CACAGCAGCG GATTCACAGG CACATGGAAC GGAGGCTGGC GATGAACAAC 
ATGATATATG AGGTCATCCA GCAAGGTCAT GACCTTCAGC AGTACATCTC GGAGGTGCAG 
GCATCAGGTA TCGAGCTGTC AGACGTGGAG GGTGGGCTGT CCTCTCAGGT GGAGGAGCTG 
CAGCGCCTCC TGCAGGACAA ACAGAAGGAG CTGCAGCAGA TTGCCGATCA CACGCATACG 
CTGCTGGAGC AGAACCTGCA GCTGAGACAC CTCCAGAGCC AGGTCAAGCA GGTGCTCGGC 
TGGATCTCAG AGGGGGAGGT GATGCTGTCC TCCTGCATGT TGAATTCCAG CTGTCTGTCA 
GAGGCGGAGC AGCTGCAGAA GGAACACGAG CGCCTCCAAC AGGCCATAGA GGCTCTGCTG 
CACGCAGACT CCCTGCAGAG GACTCATCAG GTGGCGCTGG CCCTGCAGCA GAGAGCGGAG 
CTGCTGGTCA GGTCTGGCCA CTATGATCCA GATGCAGTGA GGTCCTGCGC TCAGACTGTA 
GCGCTGCATT GGCAGACACT AATGCTCAGG ATTGAAGACA GACTCAAACT GGTCAATGCG 
TCGGCTGCTT TCTACAGGAC ATCCCAGCAG GTATGTGGGG TCCTGGAGAG TCTGGAGCAG 
GAATACCGCA GGGAGGAGGA CTGGTGTGCA GGAGGAGAGA GGCAACCAGA GCAACTGCTG 
CCGCTAATCA ATAAACACAT GGAGCAGAAG GAGGCTTTTC TGAAGGCCTG TACTCTTGCG 
AGACGGAATG CAGAAGTTTT TCTCAAGTAT ATCCATCGCA ATAGCGTCAC CATGGCAAAT 
ATCTCTGGCC ATAGCATCAC TGTCTCTGGG GTCTCTGAGG TTACTGGCCA TTCCAGAGTC 
CCTGAACAAC AGGTCAAAGG TATCCTTAGT GAGCTGCTCC AGAGGGAAAA CAGAGTTCTT 
CATTTCTGGA CTTTAAAGAA ACGCAGATTG GATCAGTGCC AGCAGTATCT GATGTTCCAA 
ACCCACGCAC AGCAGGCTCT TGATTGGTTG CAGCAGTCAG GTGACCATTA CTTGGCCACA 
CATACGTCAC CAGGTGCAAC CATGGCTGAG ACACAGGAGC TGCTGAACCA ATACAGAGAG 
TTCTGCATTT CTGCAAAGCA CACACAGGAG AAGGTTCACC TGCTGATCCA GCTGGCTGAG 
AGTATGCTGG CTAAAGGTCA CACCCACCGC ACTGAGCTCC GCCGCTGCGT TTCAACAGTG 
GATAAGCGCT ACCGTGACTT CTCTGTCAGG ATGGGACAAT ATCGCCACCT GCTAGAGACA 
GCGCTGGGAG GCTGTTCTCA GGACAATAAA GACCTGGAGC TGGAGCTGAT CCCCAATAGT 
CTCTCGGACT CTGACCCAGA GGTCAACCTA TCTGACCCAA GTCACCAAGT CAGTGATGAG 
AAAAGACGGT CCGCCCGCAA AAAGGAGTAC ATCATGGCAG AGCTCCTTCA GACAGAACGA 
GTCTATGTCA GAGACCTGCA GGAGTGCATA GAGACGTACC TGTGGGAGAT GACAAGCGGT 
TCAGAAGATG TTCCACTCGG TCTTGCCAAC AGTCACTACA CTGTGTTTGG AAACATTCAG 
GACATCTACG AGTTCCATAA CAGTATTTTC TTAAAGGAGT TAGAGAACTA CGAGCAGCTT 
CCTGAAGACG TGGGACACTG TTTCGTCACC TGGGCTGATA AGTTCCACAT GTATGTGACG 
TACTGCAGAA ACAAACCAGA CTCCAGTTTA TTGATCCAAC AGCATGGAGT GGGATTTTTT 
GAGGAGGTGC AGAGGAGACA CGGACTTGCT AACTCCATCT CCTCTGCGCT GATTAAACCG 
GTCCAGAGGA TCACCAAGTA CCAGCTGTTG CTTAAGGAGC TGCTGGCTTG CTGCGAGGAA 
GGCAAGGGGG AGATCAAGGA AGGCCTGGAC GTGATGCTGA GTGTCCCAAA GAGAGCCAAC 
GATGCAATGC ACGTCAGCAT GTTAGAAGGT CTGGAGGACG GGGTGGAGGT GCAGGGAGAG 
CTCCTCCTGC AGGACTCTTT CCTGGTCTGG GAGCCTAAGT CTCTGATAAG AAAGGGGCGG 
GACAGACACT TATTCTTGTT TGAACTGTCA CTCATCTTTA GCAAAGAGAT TAAAGACTCG 
TCCGGCCGCA CCAAGTATCT CTACAAAAGT CGCTTGAGGA CGTCAGAGCT GGGAGTGACG 
GAGCACATAG AGGGGGATCA GTGTAAGTTT GCACTGTGGG TGGGACGAAC GCCGACATCA 
GACAACAAGA CCGTCCTGAA GGCTTCATCC CTCGAGCTAA AGCAGGAGTG GGTTCGCAGT 
ATCCGACAGG TGATCCAGGA GAGGCGGGGT CACCTGCGAG GCGCACTGAG GGAGCCTATC 
CCGCTCCCAA AAACCCCCAA CCCCACGCTG GGACGACAGC GCAGCATCAC CCGCAGGGAG 
ACGAGCGAGG ACGCAGACAG CCTGGGTGAT GCCAGCAGTC AGCCTGACAC GGTTTCCATC 
GCATCACGAA CGTCGCAGAA CACTGCCGAC AGCGACAAGC TCTCTGGGGG CTGCGAGCTA 
GTGGTGGTGC TGCTGGACTT CACCTCTGGA GGACCGGGAG AGCTCAGCGT CCGCTGCGGC 
CAGACGGTGG AGCTGGTGGA GCGCTCTGCA GACCGGCCTG GCTGGTGTCT GATCAGAACC 
ACGGATCAGA CGCCCCAGCA AGAGGGTTTG CTGCCCATGA GCGCCCTCTG CCTGTCGCAC 
TCCCACAGTG CAGGTGACAT GGAGGGGCTC CTCCCAGCCT CCACCACCTC CTCTAGCACC 
TCCTCCACCT GCACCACCAC CTCCTCCTCT CTCAACTCCT CCTCCACCAC CACCTCCTCA 
TCCACCATGA GCGCAGCAAA GGATTCCAGT CTGTTCAGTT CTGAGGGTTC AGGCCTCCCG 
ACTCAGACCA GCAGTCAAAG TGCCACCTCC CCCACCGTAG GTGGACCGGG CGGAGCTCCA 
GTAGGTGCAG TAGGGTCTCC GGGGCCCAAA CGGAGCGGCT CTGGCACAGG CAACACGTTA 
AAGCGGTGGT TGACCAGCCC TGTGCGAAGG CTGAGTCAGG GAAAAGCTGA CGGACAGAAA 
AAACCTCCAA TCAGAGCCAG GAGGCGGGAC AACAAGCTCG AGCTGGCCAC GCCCCTTACC 
GTAAATACAC AGATGAAGGC AAGAAAGGAG GAGGCGGGGC AAAGTGGAGG GGAGGAGGAG 
CAAGAGGAAG AAAGCCACAC CCCATTACCT CCACCGATGC AGATTATGAA AGACCCCAAC 
AATCCAGAGG CTCTGGACTC AGATCAGGGA TCAAACGAGT CCGATACTGA ACAGAGAAAC 
AAAGCACTAA GAGGACGAAT GTTTGTTGTT AATGAGATGG TCCAATCAGA GAAGGACTAC 
GTAAAGGACC TAGGAGTGAT TGTTGAGGGC TTCATGTCCA GGCTTGAGGT CAGGGGGATC 
CCAGAAGACA TGAGGGGAAA AGACAAGATC GTGTTTGGCA ACATCCAGCA GATCTACGAC 
TGGCACCGCG ACTTCTTTCT GGTGGAGCTA GAGCGCTGCA TCCAGAACCA CGATCTGCTG 
GCCGACCTGT TCATCAGACA TGAGCGTCGT CTGCACATGT ATGTGGTTTA CTGTCAAAAC 
AAGCCCAAGT CGGAGTTCAT CGTCATTGAG TATGAGACCT TCTTTGAGGA GATCCAGCAT 
GAGATCAGCT GCAGGATGTC GATCAGTGAT TATCTGATCA AACCCATCCA GAGAATCACC 
AAGTACCAGC TACTACTGAA GGACTTCTTC AAATATACGA CCAAAGCAGG GCTGGACACT 
GAGGAGATTG AGAAAGCGGT GGAGCTGATG TCATTGGTTC CAAAGCGCTG CAACGACATG 
ATGAACCTGG GACGCCTGCA GGGATATGAG GGGAAGTTGA CGTCTCAGGG GAAGCTGCTG 
CAGCAGGAGA CCTTCTGCGT TTGGGAGCAG GACGGGGGAG TCTTGTCTCG CAGCAAAGAG 
CGCAGAGTCT TCCTCTTCGA GCAGATCATC ATCTTCAGTG AACTGCTCCG GAAAGGCTCC 
AACAATCCAG GATACCAGTT CAAGAACAGC ATCAAGGTGA GTTACCTGGC CATGCAGGAC 
AGCGTAGACG GCGATCCCTG TAAGTTTGTG CTGTGGTCCC GGGGCTCGGC AGAGCGCTTC 
ACTCTACAAG CGTCCTCCGC CAGCATCAAG ATGACCTGGG TGGAGACCAT TGCCACTCTG 
CTGGACGCCC AGAACAACTT CCTCTCTGCT CTCCAGTCAC CTATTGAGTA CCAGAGAAAA 
GAGGGCGGGA TTTCAGTGAC CCGTCCACTG TCGTCTGGTC GTCCACCGTC TGCTCCGCCC 
ACGCCTAACG GCCATGCCAC TCAACCTCCT GCTGACAGTG AGGATGACCA GGAGCTGGTT 
CAGGTGCTGC AGGACTTTGT GGCCGTGCGG GAGGACGAGA TTTCCGTGTT TCGGGGAGAG 
AAAGTCCAGA TTCTGTCGTC CAACCAGCAG GGTCAGAGTC TTGTTTACAG ACCTGCCAAC 
ACCGACTCGC CCGCCGCCGA GGGCTGGGTG CCACGAAGCG TGCTCAAAAC ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023820135.1
CDS	470..7546
Translation	MNPADGGGEAGPDGQATAGSGKGESVRAVDVLTVLREKVAFVSGGRDKRGGPILTFPARTNHDRIKQEDLSRLVTYLSTIPSEDVRARGFTVVVDMRGSKWDSVKPVLRTLQESFSSNIHTALLIKPDTFWQKHKTNLGSAKLSFETSLVSVEGLSRLIDPSQLTADLGGSLEYDHVEWTELRLGLEEFSGAATQLLTQLEQLEAGLSRIPEPQDIDEARKLLEEHTRLRNSIATAPVDALDREGKCLLQRMRLGSSHGEASNESGGGGGTWLSGGADFQSVAPKVSSLLDRLQGARSHLLQCWGDKKLHLDQALQLHLFEQDATKMSEWIAHSKELFMQSLAEVGQNHQHALDLQTQHQHFTANCMNGSVNVDSVVTVAARLAEAGHYGAERIALVSSKLQDNWKSMTSALEERSNMLAMATGFHQGAEQFLARVEGWVKLCSEGPLPSTTAELEPAIKKHQELNEEISANYTQVSESGKALLEVLQRCPTTDSDESATKPDFTAATHTIMGVLHQVMQGHHEVEGAWQHRKLRLHQRLQLCVFQQDVKQVLDWVEQHGEVFLNKHTGVGKSLHRARALQKRHDDFQQVAQNTYTNAEKLLEAADQLAQSGECEEEEIYQAARDLELRMQAFIQRVEQRKLLLDLAVSFYTHTKELSAWMEGLQKQLSSDLLVCPDTVEALQALISQQQQQQTNTQEAAMSVIREGEDLILQLRPQGSSVAHVESVLQQLEESHVQLEELFHQRKIRVDIFLQFRILHQCTLEVTAEMDAWKMDLEKQSQEFSSEKVASNRLAETNQDKLTQDKLALGQSRLVESSPEALTLAQQRIHRHMERRLAMNNMIYEVIQQGHDLQQYISEVQASGIELSDVEGGLSSQVEELQRLLQDKQKELQQIADHTHTLLEQNLQLRHLQSQVKQVLGWISEGEVMLSSCMLNSSCLSEAEQLQKEHERLQQAIEALLHADSLQRTHQVALALQQRAELLVRSGHYDPDAVRSCAQTVALHWQTLMLRIEDRLKLVNASAAFYRTSQQVCGVLESLEQEYRREEDWCAGGERQPEQLLPLINKHMEQKEAFLKACTLARRNAEVFLKYIHRNSVTMANISGHSITVSGVSEVTGHSRVPEQQVKGILSELLQRENRVLHFWTLKKRRLDQCQQYLMFQTHAQQALDWLQQSGDHYLATHTSPGATMAETQELLNQYREFCISAKHTQEKVHLLIQLAESMLAKGHTHRTELRRCVSTVDKRYRDFSVRMGQYRHLLETALGGCSQDNKDLELELIPNSLSDSDPEVNLSDPSHQVSDEKRRSARKKEYIMAELLQTERVYVRDLQECIETYLWEMTSGSEDVPLGLANSHYTVFGNIQDIYEFHNSIFLKELENYEQLPEDVGHCFVTWADKFHMYVTYCRNKPDSSLLIQQHGVGFFEEVQRRHGLANSISSALIKPVQRITKYQLLLKELLACCEEGKGEIKEGLDVMLSVPKRANDAMHVSMLEGLEDGVEVQGELLLQDSFLVWEPKSLIRKGRDRHLFLFELSLIFSKEIKDSSGRTKYLYKSRLRTSELGVTEHIEGDQCKFALWVGRTPTSDNKTVLKASSLELKQEWVRSIRQVIQERRGHLRGALREPIPLPKTPNPTLGRQRSITRRETSEDADSLGDASSQPDTVSIASRTSQNTADSDKLSGGCELVVVLLDFTSGGPGELSVRCGQTVELVERSADRPGWCLIRTTDQTPQQEGLLPMSALCLSHSHSAGDMEGLLPASTTSSSTSSTCTTTSSSLNSSSTTTSSSTMSAAKDSSLFSSEGSGLPTQTSSQSATSPTVGGPGGAPVGAVGSPGPKRSGSGTGNTLKRWLTSPVRRLSQGKADGQKKPPIRARRRDNKLELATPLTVNTQMKARKEEAGQSGGEEEQEEESHTPLPPPMQIMKDPNNPEALDSDQGSNESDTEQRNKALRGRMFVVNEMVQSEKDYVKDLGVIVEGFMSRLEVRGIPEDMRGKDKIVFGNIQQIYDWHRDFFLVELERCIQNHDLLADLFIRHERRLHMYVVYCQNKPKSEFIVIEYETFFEEIQHEISCRMSISDYLIKPIQRITKYQLLLKDFFKYTTKAGLDTEEIEKAVELMSLVPKRCNDMMNLGRLQGYEGKLTSQGKLLQQETFCVWEQDGGVLSRSKERRVFLFEQIIIFSELLRKGSNNPGYQFKNSIKVSYLAMQDSVDGDPCKFVLWSRGSAERFTLQASSASIKMTWVETIATLLDAQNNFLSALQSPIEYQRKEGGISVTRPLSSGRPPSAPPTPNGHATQPPADSEDDQELVQVLQDFVAVREDEISVFRGEKVQILSSNQQGQSLVYRPANTDSPAAEGWVPRSVLKTH

Target ORF information:

RefSeq Version	XM_023964367.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes kalirin (LOC101166941), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023964367.1

ORF Insert Sequence:

ATGAATCCGG CAGATGGAGG AGGAGAAGCG GGCCCGGACG GCCAGGCGAC GGCAGGTTCT 
GGTAAAGGGG AGTCTGTCCG GGCGGTCGAT GTCTTGACGG TGCTCAGAGA AAAAGTGGCC 
TTTGTGTCGG GTGGACGCGA CAAACGAGGT GGACCAATTC TCACCTTTCC TGCTAGGACC 
AATCACGATC GAATAAAACA GGAAGACCTC AGCCGCTTGG TTACTTATTT ATCAACTATC 
CCTAGCGAGG ATGTGCGTGC CCGTGGCTTT ACCGTTGTGG TGGATATGCG GGGCAGTAAG 
TGGGACAGCG TGAAGCCGGT TCTGCGAACA CTACAGGAGT CCTTTTCCTC AAACATCCAC 
ACAGCACTAC TCATCAAACC GGACACCTTC TGGCAGAAAC ACAAGACCAA CCTAGGCAGT 
GCCAAGCTGA GTTTCGAGAC CAGTCTGGTG TCAGTGGAGG GTTTGTCCCG ACTTATTGAT 
CCCTCTCAGC TGACCGCTGA CCTCGGAGGT TCATTGGAGT ATGACCATGT GGAGTGGACT 
GAGCTGCGTC TTGGTTTAGA GGAGTTCTCA GGGGCTGCCA CACAGCTACT AACCCAACTG 
GAGCAGCTGG AAGCCGGGCT GAGCCGCATA CCCGAGCCTC AGGACATTGA TGAGGCCCGC 
AAACTCTTGG AAGAACACAC TCGCCTCCGC AATAGCATCG CCACTGCGCC TGTTGATGCC 
CTCGACCGAG AAGGGAAGTG TTTACTTCAG CGGATGCGCC TAGGTTCATC CCACGGTGAA 
GCCTCAAATG AGAGTGGGGG TGGTGGGGGA ACTTGGTTGT CAGGAGGAGC AGATTTCCAG 
AGCGTGGCGC CGAAGGTTTC GTCTTTATTG GACCGGCTGC AGGGGGCTCG CAGTCACTTA 
CTGCAGTGCT GGGGTGATAA GAAGCTGCAC TTGGACCAGG CACTGCAACT CCACCTGTTT 
GAACAGGATG CTACCAAGAT GTCAGAGTGG ATCGCTCACA GTAAAGAGCT CTTCATGCAG 
AGTCTGGCAG AAGTCGGTCA GAACCACCAG CACGCTCTCG ATCTACAAAC GCAGCACCAG 
CATTTCACCG CCAACTGCAT GAACGGCAGT GTGAATGTGG ACAGTGTCGT TACCGTGGCA 
GCAAGGCTCG CGGAGGCCGG CCACTACGGA GCAGAGCGGA TTGCTTTGGT GTCGTCAAAG 
TTACAGGACA ACTGGAAGTC AATGACAAGT GCTCTGGAAG AGCGGAGCAA CATGCTTGCC 
ATGGCAACCG GTTTCCATCA AGGGGCAGAG CAGTTTCTAG CCCGAGTGGA GGGATGGGTT 
AAACTGTGCT CTGAAGGGCC ATTGCCATCA ACAACAGCCG AGCTGGAACC TGCAATAAAA 
AAACACCAGG AGCTCAATGA GGAGATTTCA GCCAACTACA CACAGGTCAG TGAAAGCGGT 
AAGGCATTGC TGGAGGTCCT TCAGCGTTGC CCCACAACAG ATTCTGACGA ATCAGCAACC 
AAACCAGATT TCACTGCGGC CACACACACC ATTATGGGGG TTCTACACCA GGTGATGCAG 
GGTCATCACG AAGTAGAGGG AGCATGGCAG CATCGTAAGC TCCGCCTCCA CCAGCGCCTG 
CAGTTATGTG TGTTTCAACA GGATGTTAAA CAGGTTTTGG ACTGGGTGGA GCAGCATGGA 
GAGGTTTTCC TGAACAAACA CACGGGTGTG GGGAAGTCCT TGCACCGGGC ACGGGCGCTG 
CAGAAGCGAC ACGATGACTT TCAGCAGGTT GCACAGAATA CGTACACCAA TGCAGAGAAG 
CTTCTGGAGG CAGCAGACCA GTTGGCGCAG TCTGGAGAGT GTGAGGAAGA GGAGATCTAC 
CAGGCAGCCA GAGACCTGGA GCTGCGAATG CAGGCCTTCA TTCAGCGCGT TGAACAGAGG 
AAGCTGCTGC TGGACCTCGC CGTATCCTTC TACACGCACA CAAAGGAGCT CTCTGCATGG 
ATGGAGGGCC TCCAGAAGCA GCTCTCCTCA GACCTCCTCG TGTGCCCGGA CACTGTGGAA 
GCTCTGCAGG CTTTAATCAG CCAACAGCAG CAGCAGCAGA CCAACACTCA GGAAGCTGCG 
ATGAGTGTCA TCCGGGAAGG AGAAGACCTC ATCCTGCAAC TCAGGCCTCA GGGCAGCTCG 
GTGGCCCATG TGGAATCGGT TTTGCAGCAA CTGGAAGAGT CTCATGTTCA GCTTGAGGAA 
CTTTTTCACC AGAGGAAGAT CCGAGTGGAC ATTTTCCTGC AGTTCCGCAT ACTGCACCAG 
TGCACGCTGG AGGTTACAGC TGAGATGGAT GCTTGGAAAA TGGACTTGGA AAAACAATCC 
CAGGAATTTT CCTCGGAGAA GGTCGCCTCA AATCGGCTTG CTGAAACCAA CCAGGACAAG 
CTGACCCAGG ATAAGCTGGC ACTAGGACAG TCCCGGCTTG TGGAGTCCAG CCCGGAAGCG 
TTAACACTAG CACAGCAGCG GATTCACAGG CACATGGAAC GGAGGCTGGC GATGAACAAC 
ATGATATATG AGGTCATCCA GCAAGGTCAT GACCTTCAGC AGTACATCTC GGAGGTGCAG 
GCATCAGGTA TCGAGCTGTC AGACGTGGAG GGTGGGCTGT CCTCTCAGGT GGAGGAGCTG 
CAGCGCCTCC TGCAGGACAA ACAGAAGGAG CTGCAGCAGA TTGCCGATCA CACGCATACG 
CTGCTGGAGC AGAACCTGCA GCTGAGACAC CTCCAGAGCC AGGTCAAGCA GGTGCTCGGC 
TGGATCTCAG AGGGGGAGGT GATGCTGTCC TCCTGCATGT TGAATTCCAG CTGTCTGTCA 
GAGGCGGAGC AGCTGCAGAA GGAACACGAG CGCCTCCAAC AGGCCATAGA GGCTCTGCTG 
CACGCAGACT CCCTGCAGAG GACTCATCAG GTGGCGCTGG CCCTGCAGCA GAGAGCGGAG 
CTGCTGGTCA GGTCTGGCCA CTATGATCCA GATGCAGTGA GGTCCTGCGC TCAGACTGTA 
GCGCTGCATT GGCAGACACT AATGCTCAGG ATTGAAGACA GACTCAAACT GGTCAATGCG 
TCGGCTGCTT TCTACAGGAC ATCCCAGCAG GTATGTGGGG TCCTGGAGAG TCTGGAGCAG 
GAATACCGCA GGGAGGAGGA CTGGTGTGCA GGAGGAGAGA GGCAACCAGA GCAACTGCTG 
CCGCTAATCA ATAAACACAT GGAGCAGAAG GAGGCTTTTC TGAAGGCCTG TACTCTTGCG 
AGACGGAATG CAGAAGTTTT TCTCAAGTAT ATCCATCGCA ATAGCGTCAC CATGGCAAAT 
ATCTCTGGCC ATAGCATCAC TGTCTCTGGG GTCTCTGAGG TTACTGGCCA TTCCAGAGTC 
CCTGAACAAC AGGTCAAAGG TATCCTTAGT GAGCTGCTCC AGAGGGAAAA CAGAGTTCTT 
CATTTCTGGA CTTTAAAGAA ACGCAGATTG GATCAGTGCC AGCAGTATCT GATGTTCCAA 
ACCCACGCAC AGCAGGCTCT TGATTGGTTG CAGCAGTCAG GTGACCATTA CTTGGCCACA 
CATACGTCAC CAGGTGCAAC CATGGCTGAG ACACAGGAGC TGCTGAACCA ATACAGAGAG 
TTCTGCATTT CTGCAAAGCA CACACAGGAG AAGGTTCACC TGCTGATCCA GCTGGCTGAG 
AGTATGCTGG CTAAAGGTCA CACCCACCGC ACTGAGCTCC GCCGCTGCGT TTCAACAGTG 
GATAAGCGCT ACCGTGACTT CTCTGTCAGG ATGGGACAAT ATCGCCACCT GCTAGAGACA 
GCGCTGGGAG GCTGTTCTCA GGACAATAAA GACCTGGAGC TGGAGCTGAT CCCCAATAGT 
CTCTCGGACT CTGACCCAGA GGTCAACCTA TCTGACCCAA GTCACCAAGT CAGTGATGAG 
AAAAGACGGT CCGCCCGCAA AAAGGAGTAC ATCATGGCAG AGCTCCTTCA GACAGAACGA 
GTCTATGTCA GAGACCTGCA GGAGTGCATA GAGACGTACC TGTGGGAGAT GACAAGCGGT 
TCAGAAGATG TTCCACTCGG TCTTGCCAAC AGTCACTACA CTGTGTTTGG AAACATTCAG 
GACATCTACG AGTTCCATAA CAGTATTTTC TTAAAGGAGT TAGAGAACTA CGAGCAGCTT 
CCTGAAGACG TGGGACACTG TTTCGTCACC TGGGCTGATA AGTTCCACAT GTATGTGACG 
TACTGCAGAA ACAAACCAGA CTCCAGTTTA TTGATCCAAC AGCATGGAGT GGGATTTTTT 
GAGGAGGTGC AGAGGAGACA CGGACTTGCT AACTCCATCT CCTCTGCGCT GATTAAACCG 
GTCCAGAGGA TCACCAAGTA CCAGCTGTTG CTTAAGGAGC TGCTGGCTTG CTGCGAGGAA 
GGCAAGGGGG AGATCAAGGA AGGCCTGGAC GTGATGCTGA GTGTCCCAAA GAGAGCCAAC 
GATGCAATGC ACGTCAGCAT GTTAGAAGGT CTGGAGGACG GGGTGGAGGT GCAGGGAGAG 
CTCCTCCTGC AGGACTCTTT CCTGGTCTGG GAGCCTAAGT CTCTGATAAG AAAGGGGCGG 
GACAGACACT TATTCTTGTT TGAACTGTCA CTCATCTTTA GCAAAGAGAT TAAAGACTCG 
TCCGGCCGCA CCAAGTATCT CTACAAAAGT CGCTTGAGGA CGTCAGAGCT GGGAGTGACG 
GAGCACATAG AGGGGGATCA GTGTAAGTTT GCACTGTGGG TGGGACGAAC GCCGACATCA 
GACAACAAGA CCGTCCTGAA GGCTTCATCC CTCGAGCTAA AGCAGGAGTG GGTTCGCAGT 
ATCCGACAGG TGATCCAGGA GAGGCGGGGT CACCTGCGAG GCGCACTGAG GGAGCCTATC 
CCGCTCCCAA AAACCCCCAA CCCCACGCTG GGACGACAGC GCAGCATCAC CCGCAGGGAG 
ACGAGCGAGG ACGCAGACAG CCTGGGTGAT GCCAGCAGTC AGCCTGACAC GGTTTCCATC 
GCATCACGAA CGTCGCAGAA CACTGCCGAC AGCGACAAGC TCTCTGGGGG CTGCGAGCTA 
GTGGTGGTGC TGCTGGACTT CACCTCTGGA GGACCGGGAG AGCTCAGCGT CCGCTGCGGC 
CAGACGGTGG AGCTGGTGGA GCGCTCTGCA GACCGGCCTG GCTGGTGTCT GATCAGAACC 
ACGGATCAGA CGCCCCAGCA AGAGGGTTTG CTGCCCATGA GCGCCCTCTG CCTGTCGCAC 
TCCCACAGTG CAGGTGACAT GGAGGGGCTC CTCCCAGCCT CCACCACCTC CTCTAGCACC 
TCCTCCACCT GCACCACCAC CTCCTCCTCT CTCAACTCCT CCTCCACCAC CACCTCCTCA 
TCCACCATGA GCGCAGCAAA GGATTCCAGT CTGTTCAGTT CTGAGGGTTC AGGCCTCCCG 
ACTCAGACCA GCAGTCAAAG TGCCACCTCC CCCACCGTAG GTGGACCGGG CGGAGCTCCA 
GTAGGTGCAG TAGGGTCTCC GGGGCCCAAA CGGAGCGGCT CTGGCACAGG CAACACGTTA 
AAGCGGTGGT TGACCAGCCC TGTGCGAAGG CTGAGTCAGG GAAAAGCTGA CGGACAGAAA 
AAACCTCCAA TCAGAGCCAG GAGGCGGGAC AACAAGCTCG AGCTGGCCAC GCCCCTTACC 
GTAAATACAC AGATGAAGGC AAGAAAGGAG GAGGCGGGGC AAAGTGGAGG GGAGGAGGAG 
CAAGAGGAAG AAAGCCACAC CCCATTACCT CCACCGATGC AGATTATGAA AGACCCCAAC 
AATCCAGAGG CTCTGGACTC AGATCAGGGA TCAAACGAGT CCGATACTGA ACAGAGAAAC 
AAAGCACTAA GAGGACGAAT GTTTGTTGTT AATGAGATGG TCCAATCAGA GAAGGACTAC 
GTAAAGGACC TAGGAGTGAT TGTTGAGGGC TTCATGTCCA GGCTTGAGGT CAGGGGGATC 
CCAGAAGACA TGAGGGGAAA AGACAAGATC GTGTTTGGCA ACATCCAGCA GATCTACGAC 
TGGCACCGCG ACTTCTTTCT GGTGGAGCTA GAGCGCTGCA TCCAGAACCA CGATCTGCTG 
GCCGACCTGT TCATCAGACA TGAGCGTCGT CTGCACATGT ATGTGGTTTA CTGTCAAAAC 
AAGCCCAAGT CGGAGTTCAT CGTCATTGAG TATGAGACCT TCTTTGAGGA GATCCAGCAT 
GAGATCAGCT GCAGGATGTC GATCAGTGAT TATCTGATCA AACCCATCCA GAGAATCACC 
AAGTACCAGC TACTACTGAA GGACTTCTTC AAATATACGA CCAAAGCAGG GCTGGACACT 
GAGGAGATTG AGAAAGCGGT GGAGCTGATG TCATTGGTTC CAAAGCGCTG CAACGACATG 
ATGAACCTGG GACGCCTGCA GGGATATGAG GGGAAGTTGA CGTCTCAGGG GAAGCTGCTG 
CAGCAGGAGA CCTTCTGCGT TTGGGAGCAG GACGGGGGAG TCTTGTCTCG CAGCAAAGAG 
CGCAGAGTCT TCCTCTTCGA GCAGATCATC ATCTTCAGTG AACTGCTCCG GAAAGGCTCC 
AACAATCCAG GATACCAGTT CAAGAACAGC ATCAAGGTGA GTTACCTGGC CATGCAGGAC 
AGCGTAGACG GCGATCCCTG TAAGTTTGTG CTGTGGTCCC GGGGCTCGGC AGAGCGCTTC 
ACTCTACAAG CGTCCTCCGC CAGCATCAAG ATGACCTGGG TGGAGACCAT TGCCACTCTG 
CTGGACGCCC AGAACAACTT CCTCTCTGCT CTCCAGTCAC CTATTGAGTA CCAGAGAAAA 
GAGGGCGGGA TTTCAGTGAC CCGTCCACTG TCGTCTGGTC GTCCACCGTC TGCTCCGCCC 
ACGCCTAACG GCCATGCCAC TCAACCTCCT GCTGACAGTG AGGATGACCA GGAGCTGGTT 
CAGGTGCTGC AGGACTTTGT GGCCGTGCGG GAGGACGAGA TTTCCGTGTT TCGGGGAGAG 
AAAGTCCAGA TTCTGTCGTC CAACCAGCAG GGTCAGAGTC TTGTTTACAG ACCTGCCAAC 
ACCGACTCGC CCGCCGCCGA GGGCTGGGTG CCACGAAGCG TGCTCAAAAC ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101166941 ( XM_020707971.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020563630.1 Oryzias latipes kalirin-like (LOC101166941), transcript variant X1, mRNA.

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CloneID	OJa74578
Clone ID Related Accession (Same CDS sequence)	XM_020707971.1
Accession Version	XM_020707971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	kalirin-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.1) and transcript sequence (GFIO01025530.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 506 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAATCCGG CAGATGGAGG AGGAGAAGCG GGCCCGGACG GCCAGGCGAC GGCAGGTTCT 
GGTAAAGGGG AGTCTGTCCG GGCGGTCGAT GTCTTGACGG TGCTCAGAGA AAAAGTGGCC 
TTTGTGTCGG GTGGACGCGA CAAACGAGGT GGACCAATTC TCACCTTTCC TGCTAGGACC 
AATCACGATC GAATAAAACA GGAAGACCTC AGCCGCTTGG TTACTTATTT ATCAACTATC 
CCTAGCGAGG ATGTGCGTGC CCGTGGCTTT ACCGTTGTGG TGGATATGCG GGGCAGTAAG 
TGGGACAGCG TGAAGCCGGT TCTGCGAACA CTACAGGAGT CCTTTTCCTC AAACATCCAC 
ACAGCACTAC TCATCAAACC GGACACCTTC TGGCAGAAAC ACAAGACCAA CCTAGGCAGT 
GCCAAGCTGA GTTTCGAGAC CAGTCTGGTG TCAGTGGAGG GTTTGTCCCG ACTTATTGAT 
CCCTCTCAGC TGACCGCTGA CCTCGGAGGT TCATTGGAGT ATGACCATGT GGAGTGGACT 
GAGCTGCGTC TTGGTTTAGA GGAGTTCTCA GGGGCTGCCA CACAGCTACT AACCCAACTG 
GAGCAGCTGG AAGCCGGGCT GAGCCGCATA CCCGAGCCTC AGGACATTGA TGAGGCCCGC 
AAACTCTTGG AAGAACACAC TCGCCTCCGC AATAGCATCG CCACTGCGCC TGTTGATGCC 
CTCGACCGAG AAGGGAAGTG TTTACTTCAG CGGATGCGCC TAGGTTCATC CCACGGTGAA 
GCCTCAAATG AGAGTGGGGG TGGTGGGGGA ACTTGGTTGT CAGGAGGAGC AGATTTCCAG 
AGCGTGGCGC CGAAGGTTTC GTCTTTATTG GACCGGCTGC AGGGGGCTCG CAGTCACTTA 
CTGCAGTGCT GGGGTGATAA GAAGCTGCAC TTGGACCAGG CACTGCAACT CCACCTGTTT 
GAACAGGATG CTACCAAGAT GTCAGAGTGG ATCGCTCACA GTAAAGAGCT CTTCATGCAG 
AGTCTGGCAG AAGTCGGTCA GAACCACCAG CACGCTCTCG ATCTACAAAC GCAGCACCAG 
CATTTCACCG CCAACTGCAT GAACGGCAGT GTGAATGTGG ACAGTGTCGT TACCGTGGCA 
GCAAGGCTCG CGGAGGCCGG CCACTACGGA GCAGAGCGGA TTGCTTTGGT GTCGTCAAAG 
TTACAGGACA ACTGGAAGTC AATGACAAGT GCTCTGGAAG AGCGGAGCAA CATGCTTGCC 
ATGGCAACCG GTTTCCATCA AGGGGCAGAG CAGTTTCTAG CCCGAGTGGA GGGATGGGTT 
AAACTGTGCT CTGAAGGGCC ATTGCCATCA ACAACAGCCG AGCTGGAACC TGCAATAAAA 
AAACACCAGG AGCTCAATGA GGAGATTTCA GCCAACTACA CACAGGTCAG TGAAAGCGGT 
AAGGCATTGC TGGAGGTCCT TCAGCGTTGC CCCACAACAG ATTCTGACGA ATCAGCAACC 
AAACCAGATT TCACTGCGGC CACACACACC ATTATGGGGG TTCTACACCA GGTGATGCAG 
GGTCATCACG AAGTAGAGGG AGCATGGCAG CATCGTAAGC TCCGCCTCCA CCAGCGCCTG 
CAGTTATGTG TGTTTCAACA GGATGTTAAA CAGGTTTTGG ACTGGGTGGA GCAGCATGGA 
GAGGTTTTCC TGAACAAACA CACGGGTGTG GGGAAGTCCT TGCACCGGGC ACGGGCGCTG 
CAGAAGCGAC ACGATGACTT TCAGCAGGTT GCACAGAATA CGTACACCAA TGCAGAGAAG 
CTTCTGGAGG CAGCAGACCA GTTGGCGCAG TCTGGAGAGT GTGAGGAAGA GGAGATCTAC 
CAGGCAGCCA GAGACCTGGA GCTGCGAATG CAGGCCTTCA TTCAGCGCGT TGAACAGAGG 
AAGCTGCTGC TGGACCTCGC CGTATCCTTC TACACGCACA CAAAGGAGCT CTCTGCATGG 
ATGGAGGGCC TCCAGAAGCA GCTCTCCTCA GACCTCCTCG TGTGCCCGGA CACTGTGGAA 
GCTCTGCAGG CTTTAATCAG CCAACAGCAG CAGCAGCAGA CCAACACTCA GGAAGCTGCG 
ATGAGTGTCA TCCGGGAAGG AGAAGACCTC ATCCTGCAAC TCAGGCCTCA GGGCAGCTCG 
GTGGCCCATG TGGAATCGGT TTTGCAGCAA CTGGAAGAGT CTCATGTTCA GCTTGAGGAA 
CTTTTTCACC AGAGGAAGAT CCGAGTGGAC ATTTTCCTGC AGTTCCGCAT ACTGCACCAG 
TGCACGCTGG AGGTTACAGC TGAGATGGAT GCTTGGAAAA TGGACTTGGA AAAACAATCC 
CAGGAATTTT CCTCGGAGAA GGTCGCCTCA AATCGGCTTG CTGAAACCAA CCAGGACAAG 
CTGACCCAGG ATAAGCTGGC ACTAGGACAG TCCCGGCTTG TGGAGTCCAG CCCGGAAGCG 
TTAACACTAG CACAGCAGCG GATTCACAGG CACATGGAAC GGAGGCTGGC GATGAACAAC 
ATGATATATG AGGTCATCCA GCAAGGTCAT GACCTTCAGC AGTACATCTC GGAGGTGCAG 
GCATCAGGTA TCGAGCTGTC AGACGTGGAG GGTGGGCTGT CCTCTCAGGT GGAGGAGCTG 
CAGCGCCTCC TGCAGGACAA ACAGAAGGAG CTGCAGCAGA TTGCCGATCA CACGCATACG 
CTGCTGGAGC AGAACCTGCA GCTGAGACAC CTCCAGAGCC AGGTCAAGCA GGTGCTCGGC 
TGGATCTCAG AGGGGGAGGT GATGCTGTCC TCCTGCATGT TGAATTCCAG CTGTCTGTCA 
GAGGCGGAGC AGCTGCAGAA GGAACACGAG CGCCTCCAAC AGGCCATAGA GGCTCTGCTG 
CACGCAGACT CCCTGCAGAG GACTCATCAG GTGGCGCTGG CCCTGCAGCA GAGAGCGGAG 
CTGCTGGTCA GGTCTGGCCA CTATGATCCA GATGCAGTGA GGTCCTGCGC TCAGACTGTA 
GCGCTGCATT GGCAGACACT AATGCTCAGG ATTGAAGACA GACTCAAACT GGTCAATGCG 
TCGGCTGCTT TCTACAGGAC ATCCCAGCAG GTATGTGGGG TCCTGGAGAG TCTGGAGCAG 
GAATACCGCA GGGAGGAGGA CTGGTGTGCA GGAGGAGAGA GGCAACCAGA GCAACTGCTG 
CCGCTAATCA ATAAACACAT GGAGCAGAAG GAGGCTTTTC TGAAGGCCTG TACTCTTGCG 
AGACGGAATG CAGAAGTTTT TCTCAAGTAT ATCCATCGCA ATAGCGTCAC CATGGCAAAT 
ATCTCTGGCC ATAGCATCAC TGTCTCTGGG GTCTCTGAGG TTACTGGCCA TTCCAGAGTC 
CCTGAACAAC AGGTCAAAGG TATCCTTAGT GAGCTGCTCC AGAGGGAAAA CAGAGTTCTT 
CATTTCTGGA CTTTAAAGAA ACGCAGATTG GATCAGTGCC AGCAGTATCT GATGTTCCAA 
ACCCACGCAC AGCAGGCTCT TGATTGGTTG CAGCAGTCAG GTGACCATTA CTTGGCCACA 
CATACGTCAC CAGGTGCAAC CATGGCTGAG ACACAGGAGC TGCTGAACCA ATACAGAGAG 
TTCTGCATTT CTGCAAAGGT GCACACACAG GAGAAGGTTC ACCTGCTGAT CCAGCTGGCT 
GAGAGTATGC TGGCTAAAGG TCACACCCAC CGCACTGAGC TCCGCCGCTG CGTTTCAACA 
GTGGATAAGC GCTACCGTGA CTTCTCTGTC AGGATGGGAC AATATCGCCA CCTGCTAGAG 
ACAGCGCTGG GAGGCTGTTC TCAGGACAAT AAAGACCTGG AGCTGGAGCT GATCCCCAAT 
AGTCTCTCGG ACTCTGACCC AGAGGTCAAC CTATCTGACC CAAGTCACCA AGTCAGTGAT 
GAGAAAAGAC GGTCCGCCCG CAAAAAGGAG TACATCATGG CAGAGCTCCT TCAGACAGAA 
CGAGTCTATG TCAGAGACCT GCAGGAGTGC ATAGAGACGT ACCTGTGGGA GATGACAAGC 
GGTTCAGAAG ATGTTCCACT CGGTCTTGCC AACAGTCACT ACACTGTGTT TGGAAACATT 
CAGGACATCT ACGAGTTCCA TAACAGTATT TTCTTAAAGG AGTTAGAGAA CTACGAGCAG 
CTTCCTGAAG ACGTGGGACA CTGTTTCGTC ACCTGGGCTG ATAAGTTCCA CATGTATGTG 
ACGTACTGCA GAAACAAACC AGACTCCAGT TTATTGATCC AACAGCATGG AGTGGGATTT 
TTTGAGGAGG TGCAGAGGAG ACACGGACTT GCTAACTCCA TCTCCTCTGC GCTGATTAAA 
CCGGTCCAGA GGATCACCAA GTACCAGCTG TTGCTTAAGG AGCTGCTGGC TTGCTGCGAG 
GAAGGCAAGG GGGAGATCAA GGAAGGCCTG GACGTGATGC TGAGTGTCCC AAAGAGAGCC 
AACGATGCAA TGCACGTCAG CATGTTAGAA GGTCTGGAGG ACGGGGTGGA GGTGCAGGGA 
GAGCTCCTCC TGCAGGACTC TTTCCTGGTC TGGGAGCCTA AGTCTCTGAT AAGAAAGGGG 
CGGGACAGAC ACTTATTCTT GTTTGAACTG TCACTCATCT TTAGCAAAGA GATTAAAGAC 
TCGTCCGGCC GCACCAAGTA TCTCTACAAA AGTCGCTTGA GGACGTCAGA GCTGGGAGTG 
ACGGAGCACA TAGAGGGGGA TCAGTGTAAG TTTGCACTGT GGGTGGGACG AACGCCGACA 
TCAGACAACA AGACCGTCCT GAAGGCTTCA TCCCTCGAGC TAAAGCAGGA GTGGGTTCGC 
AGTATCCGAC AGGTGATCCA GGAGAGGCGG GGTCACCTGC GAGGCGCACT GAGGGAGCCT 
ATCCCGCTCC CAAAAACCCC CAACCCCACG CTGGGACGAC AGCGCAGCAT CACCCGCAGG 
GAGACGAGCG AGGACGCAGA CAGCCTGGGT GATGCCAGCA GTCAGCCTGA CACGGTTTCC 
ATCGCATCAC GAACGTCGCA GAACACTGCC GACAGCGACA AGCTCTCTGG GGGCTGCGAG 
CTAGTGGTGG TGCTGCTGGA CTTCACCTCT GGAGGACCGG GAGAGCTCAG CGTCCGCTGC 
GGCCAGACGG TGGAGCTGGT GGAGCGCTCT GCAGACCGGC CTGGCTGGTG TCTGATCAGA 
ACCACGGATC AGACGCCCCA GCAAGAGGGT TTGCTGCCCA TGAGCGCCCT CTGCCTGTCG 
CACTCCCACA GTGCAGGTGA CATGGAGGGG CTCCTCCCAG CCTCCACCAC CTCCTCTAGC 
ACCTCCTCCA CCTGCACCAC CACCTCCTCC TCTCTCAACT CCTCCTCCAC CACCACCTCC 
TCATCCACCA TGAGCGCAGC AAAGGATTCC AGTCTGTTCA GTTCTGAGGG TTCAGGCCTC 
CCGACTCAGA CCAGCAGTCA AAGTGCCACC TCCCCCACCG TAGGTGGACC GGGCGGAGCT 
CCAGTAGGTG CAGTAGGGTC TCCGGGGCCC AAACGGAGCG GCTCTGGCAC AGGCAACACG 
TTAAAGCGGT GGTTGACCAG CCCTGTGCGA AGGCTGAGTC AGGGAAAAGC TGACGGACAG 
AAAAAACCTC CAATCAGAGC CAGGAGGCGG GACAACAAGC TCGAGCTGGC CACGCCCCTT 
ACCGTAAATA CACAGATGCA GAAGGCAAGA AAGGAGGAGG CGGGGCAAAG TGGAGGGGAG 
GAGGAGCAAG AGGAAGAAAG CCACACCCCA TTACCTCCAC CGATGCAGAT TATGAAAGAC 
CCCAACAATC CAGAGGCTCT GGACTCAGAT CAGGGATCAA ACGAGTCCGA TACTGAACAG 
AGAAACAAAG CACTAAGAGG ACGAATGTTT GTTGTTAATG AGATGGTCCA ATCAGAGAAG 
GACTACGTAA AGGACCTAGG AGTGATTGTT GAGGGCTTCA TGTCCAGGCT TGAGGTCAGG 
GGGATCCCAG AAGACATGAG GGGAAAAGAC AAGATCGTGT TTGGCAACAT CCAGCAGATC 
TACGACTGGC ACCGCGACTT CTTTCTGGTG GAGCTAGAGC GCTGCATCCA GAACCACGAT 
CTGCTGGCCG ACCTGTTCAT CAGACATGAG CGTCGTCTGC ACATGTATGT GGTTTACTGT 
CAAAACAAGC CCAAGTCGGA GTTCATCGTC ATTGAGTATG AGACCTTCTT TGAGGAGATC 
CAGCATGAGA TCAGCTGCAG GATGTCGATC AGTGATTATC TGATCAAACC CATCCAGAGA 
ATCACCAAGT ACCAGCTACT ACTGAAGGAC TTCTTCAAAT ATACGACCAA AGCAGGGCTG 
GACACTGAGG AGATTGAGAA AGCGGTGGAG CTGATGTCAT TGGTTCCAAA GCGCTGCAAC 
GACATGATGA ACCTGGGACG CCTGCAGGGA TATGAGGGGA AGTTGACGTC TCAGGGGAAG 
CTGCTGCAGC AGGAGACCTT CTGCGTTTGG GAGCAGGACG GGGGAGTCTT GTCTCGCAGC 
AAAGAGCGCA GAGTCTTCCT CTTCGAGCAG ATCATCATCT TCAGTGAACT GCTCCGGAAA 
GGCTCCAACA ATCCAGGATA CCAGTTCAAG AACAGCATCA AGGTGAGTTA CCTGGCCATG 
CAGGACAGCG TAGACGGCGA TCCCTGTAAG TTTGTGCTGT GGTCCCGGGG CTCGGCAGAG 
CGCTTCACTC TACAAGCGTC CTCCGCCAGC ATCAAGATGA CCTGGGTGGA GACCATTGCC 
ACTCTGCTGG ACGCCCAGAA CAACTTCCTC TCTGCTCTCC AGTCACCTAT TGAGTACCAG 
AGAAAAGAGG GCGGGATTTC AGTGACCCGT CCACTGTCGT CTGGTCGTCC ACCGTCTGCT 
CCGCCCACGC CTAACGGCCA TGCCACTCAA CCTCCTGCTG ACAGTGAGGA TGACCAGGAG 
CTGGTTCAGG TGCTGCAGGA CTTTGTGGCC GTGCGGGAGG ACGAGATTTC CGTGTTTCGG 
GGAGAGAAAG TCCAGATTCT GTCGTCCAAC CAGCAGGGTC AGAGTCTTGT TTACAGACCT 
GCCAACACCG ACTCGCCCGC CGCCGAGGGC TGGGTGCCAC GAAGCGTGCT CAAAACACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020563630.1
CDS	452..7534
Translation	MNPADGGGEAGPDGQATAGSGKGESVRAVDVLTVLREKVAFVSGGRDKRGGPILTFPARTNHDRIKQEDLSRLVTYLSTIPSEDVRARGFTVVVDMRGSKWDSVKPVLRTLQESFSSNIHTALLIKPDTFWQKHKTNLGSAKLSFETSLVSVEGLSRLIDPSQLTADLGGSLEYDHVEWTELRLGLEEFSGAATQLLTQLEQLEAGLSRIPEPQDIDEARKLLEEHTRLRNSIATAPVDALDREGKCLLQRMRLGSSHGEASNESGGGGGTWLSGGADFQSVAPKVSSLLDRLQGARSHLLQCWGDKKLHLDQALQLHLFEQDATKMSEWIAHSKELFMQSLAEVGQNHQHALDLQTQHQHFTANCMNGSVNVDSVVTVAARLAEAGHYGAERIALVSSKLQDNWKSMTSALEERSNMLAMATGFHQGAEQFLARVEGWVKLCSEGPLPSTTAELEPAIKKHQELNEEISANYTQVSESGKALLEVLQRCPTTDSDESATKPDFTAATHTIMGVLHQVMQGHHEVEGAWQHRKLRLHQRLQLCVFQQDVKQVLDWVEQHGEVFLNKHTGVGKSLHRARALQKRHDDFQQVAQNTYTNAEKLLEAADQLAQSGECEEEEIYQAARDLELRMQAFIQRVEQRKLLLDLAVSFYTHTKELSAWMEGLQKQLSSDLLVCPDTVEALQALISQQQQQQTNTQEAAMSVIREGEDLILQLRPQGSSVAHVESVLQQLEESHVQLEELFHQRKIRVDIFLQFRILHQCTLEVTAEMDAWKMDLEKQSQEFSSEKVASNRLAETNQDKLTQDKLALGQSRLVESSPEALTLAQQRIHRHMERRLAMNNMIYEVIQQGHDLQQYISEVQASGIELSDVEGGLSSQVEELQRLLQDKQKELQQIADHTHTLLEQNLQLRHLQSQVKQVLGWISEGEVMLSSCMLNSSCLSEAEQLQKEHERLQQAIEALLHADSLQRTHQVALALQQRAELLVRSGHYDPDAVRSCAQTVALHWQTLMLRIEDRLKLVNASAAFYRTSQQVCGVLESLEQEYRREEDWCAGGERQPEQLLPLINKHMEQKEAFLKACTLARRNAEVFLKYIHRNSVTMANISGHSITVSGVSEVTGHSRVPEQQVKGILSELLQRENRVLHFWTLKKRRLDQCQQYLMFQTHAQQALDWLQQSGDHYLATHTSPGATMAETQELLNQYREFCISAKVHTQEKVHLLIQLAESMLAKGHTHRTELRRCVSTVDKRYRDFSVRMGQYRHLLETALGGCSQDNKDLELELIPNSLSDSDPEVNLSDPSHQVSDEKRRSARKKEYIMAELLQTERVYVRDLQECIETYLWEMTSGSEDVPLGLANSHYTVFGNIQDIYEFHNSIFLKELENYEQLPEDVGHCFVTWADKFHMYVTYCRNKPDSSLLIQQHGVGFFEEVQRRHGLANSISSALIKPVQRITKYQLLLKELLACCEEGKGEIKEGLDVMLSVPKRANDAMHVSMLEGLEDGVEVQGELLLQDSFLVWEPKSLIRKGRDRHLFLFELSLIFSKEIKDSSGRTKYLYKSRLRTSELGVTEHIEGDQCKFALWVGRTPTSDNKTVLKASSLELKQEWVRSIRQVIQERRGHLRGALREPIPLPKTPNPTLGRQRSITRRETSEDADSLGDASSQPDTVSIASRTSQNTADSDKLSGGCELVVVLLDFTSGGPGELSVRCGQTVELVERSADRPGWCLIRTTDQTPQQEGLLPMSALCLSHSHSAGDMEGLLPASTTSSSTSSTCTTTSSSLNSSSTTTSSSTMSAAKDSSLFSSEGSGLPTQTSSQSATSPTVGGPGGAPVGAVGSPGPKRSGSGTGNTLKRWLTSPVRRLSQGKADGQKKPPIRARRRDNKLELATPLTVNTQMQKARKEEAGQSGGEEEQEEESHTPLPPPMQIMKDPNNPEALDSDQGSNESDTEQRNKALRGRMFVVNEMVQSEKDYVKDLGVIVEGFMSRLEVRGIPEDMRGKDKIVFGNIQQIYDWHRDFFLVELERCIQNHDLLADLFIRHERRLHMYVVYCQNKPKSEFIVIEYETFFEEIQHEISCRMSISDYLIKPIQRITKYQLLLKDFFKYTTKAGLDTEEIEKAVELMSLVPKRCNDMMNLGRLQGYEGKLTSQGKLLQQETFCVWEQDGGVLSRSKERRVFLFEQIIIFSELLRKGSNNPGYQFKNSIKVSYLAMQDSVDGDPCKFVLWSRGSAERFTLQASSASIKMTWVETIATLLDAQNNFLSALQSPIEYQRKEGGISVTRPLSSGRPPSAPPTPNGHATQPPADSEDDQELVQVLQDFVAVREDEISVFRGEKVQILSSNQQGQSLVYRPANTDSPAAEGWVPRSVLKTH

Target ORF information:

RefSeq Version	XM_020707971.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes kalirin-like (LOC101166941), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020707971.1

ORF Insert Sequence:

ATGAATCCGG CAGATGGAGG AGGAGAAGCG GGCCCGGACG GCCAGGCGAC GGCAGGTTCT 
GGTAAAGGGG AGTCTGTCCG GGCGGTCGAT GTCTTGACGG TGCTCAGAGA AAAAGTGGCC 
TTTGTGTCGG GTGGACGCGA CAAACGAGGT GGACCAATTC TCACCTTTCC TGCTAGGACC 
AATCACGATC GAATAAAACA GGAAGACCTC AGCCGCTTGG TTACTTATTT ATCAACTATC 
CCTAGCGAGG ATGTGCGTGC CCGTGGCTTT ACCGTTGTGG TGGATATGCG GGGCAGTAAG 
TGGGACAGCG TGAAGCCGGT TCTGCGAACA CTACAGGAGT CCTTTTCCTC AAACATCCAC 
ACAGCACTAC TCATCAAACC GGACACCTTC TGGCAGAAAC ACAAGACCAA CCTAGGCAGT 
GCCAAGCTGA GTTTCGAGAC CAGTCTGGTG TCAGTGGAGG GTTTGTCCCG ACTTATTGAT 
CCCTCTCAGC TGACCGCTGA CCTCGGAGGT TCATTGGAGT ATGACCATGT GGAGTGGACT 
GAGCTGCGTC TTGGTTTAGA GGAGTTCTCA GGGGCTGCCA CACAGCTACT AACCCAACTG 
GAGCAGCTGG AAGCCGGGCT GAGCCGCATA CCCGAGCCTC AGGACATTGA TGAGGCCCGC 
AAACTCTTGG AAGAACACAC TCGCCTCCGC AATAGCATCG CCACTGCGCC TGTTGATGCC 
CTCGACCGAG AAGGGAAGTG TTTACTTCAG CGGATGCGCC TAGGTTCATC CCACGGTGAA 
GCCTCAAATG AGAGTGGGGG TGGTGGGGGA ACTTGGTTGT CAGGAGGAGC AGATTTCCAG 
AGCGTGGCGC CGAAGGTTTC GTCTTTATTG GACCGGCTGC AGGGGGCTCG CAGTCACTTA 
CTGCAGTGCT GGGGTGATAA GAAGCTGCAC TTGGACCAGG CACTGCAACT CCACCTGTTT 
GAACAGGATG CTACCAAGAT GTCAGAGTGG ATCGCTCACA GTAAAGAGCT CTTCATGCAG 
AGTCTGGCAG AAGTCGGTCA GAACCACCAG CACGCTCTCG ATCTACAAAC GCAGCACCAG 
CATTTCACCG CCAACTGCAT GAACGGCAGT GTGAATGTGG ACAGTGTCGT TACCGTGGCA 
GCAAGGCTCG CGGAGGCCGG CCACTACGGA GCAGAGCGGA TTGCTTTGGT GTCGTCAAAG 
TTACAGGACA ACTGGAAGTC AATGACAAGT GCTCTGGAAG AGCGGAGCAA CATGCTTGCC 
ATGGCAACCG GTTTCCATCA AGGGGCAGAG CAGTTTCTAG CCCGAGTGGA GGGATGGGTT 
AAACTGTGCT CTGAAGGGCC ATTGCCATCA ACAACAGCCG AGCTGGAACC TGCAATAAAA 
AAACACCAGG AGCTCAATGA GGAGATTTCA GCCAACTACA CACAGGTCAG TGAAAGCGGT 
AAGGCATTGC TGGAGGTCCT TCAGCGTTGC CCCACAACAG ATTCTGACGA ATCAGCAACC 
AAACCAGATT TCACTGCGGC CACACACACC ATTATGGGGG TTCTACACCA GGTGATGCAG 
GGTCATCACG AAGTAGAGGG AGCATGGCAG CATCGTAAGC TCCGCCTCCA CCAGCGCCTG 
CAGTTATGTG TGTTTCAACA GGATGTTAAA CAGGTTTTGG ACTGGGTGGA GCAGCATGGA 
GAGGTTTTCC TGAACAAACA CACGGGTGTG GGGAAGTCCT TGCACCGGGC ACGGGCGCTG 
CAGAAGCGAC ACGATGACTT TCAGCAGGTT GCACAGAATA CGTACACCAA TGCAGAGAAG 
CTTCTGGAGG CAGCAGACCA GTTGGCGCAG TCTGGAGAGT GTGAGGAAGA GGAGATCTAC 
CAGGCAGCCA GAGACCTGGA GCTGCGAATG CAGGCCTTCA TTCAGCGCGT TGAACAGAGG 
AAGCTGCTGC TGGACCTCGC CGTATCCTTC TACACGCACA CAAAGGAGCT CTCTGCATGG 
ATGGAGGGCC TCCAGAAGCA GCTCTCCTCA GACCTCCTCG TGTGCCCGGA CACTGTGGAA 
GCTCTGCAGG CTTTAATCAG CCAACAGCAG CAGCAGCAGA CCAACACTCA GGAAGCTGCG 
ATGAGTGTCA TCCGGGAAGG AGAAGACCTC ATCCTGCAAC TCAGGCCTCA GGGCAGCTCG 
GTGGCCCATG TGGAATCGGT TTTGCAGCAA CTGGAAGAGT CTCATGTTCA GCTTGAGGAA 
CTTTTTCACC AGAGGAAGAT CCGAGTGGAC ATTTTCCTGC AGTTCCGCAT ACTGCACCAG 
TGCACGCTGG AGGTTACAGC TGAGATGGAT GCTTGGAAAA TGGACTTGGA AAAACAATCC 
CAGGAATTTT CCTCGGAGAA GGTCGCCTCA AATCGGCTTG CTGAAACCAA CCAGGACAAG 
CTGACCCAGG ATAAGCTGGC ACTAGGACAG TCCCGGCTTG TGGAGTCCAG CCCGGAAGCG 
TTAACACTAG CACAGCAGCG GATTCACAGG CACATGGAAC GGAGGCTGGC GATGAACAAC 
ATGATATATG AGGTCATCCA GCAAGGTCAT GACCTTCAGC AGTACATCTC GGAGGTGCAG 
GCATCAGGTA TCGAGCTGTC AGACGTGGAG GGTGGGCTGT CCTCTCAGGT GGAGGAGCTG 
CAGCGCCTCC TGCAGGACAA ACAGAAGGAG CTGCAGCAGA TTGCCGATCA CACGCATACG 
CTGCTGGAGC AGAACCTGCA GCTGAGACAC CTCCAGAGCC AGGTCAAGCA GGTGCTCGGC 
TGGATCTCAG AGGGGGAGGT GATGCTGTCC TCCTGCATGT TGAATTCCAG CTGTCTGTCA 
GAGGCGGAGC AGCTGCAGAA GGAACACGAG CGCCTCCAAC AGGCCATAGA GGCTCTGCTG 
CACGCAGACT CCCTGCAGAG GACTCATCAG GTGGCGCTGG CCCTGCAGCA GAGAGCGGAG 
CTGCTGGTCA GGTCTGGCCA CTATGATCCA GATGCAGTGA GGTCCTGCGC TCAGACTGTA 
GCGCTGCATT GGCAGACACT AATGCTCAGG ATTGAAGACA GACTCAAACT GGTCAATGCG 
TCGGCTGCTT TCTACAGGAC ATCCCAGCAG GTATGTGGGG TCCTGGAGAG TCTGGAGCAG 
GAATACCGCA GGGAGGAGGA CTGGTGTGCA GGAGGAGAGA GGCAACCAGA GCAACTGCTG 
CCGCTAATCA ATAAACACAT GGAGCAGAAG GAGGCTTTTC TGAAGGCCTG TACTCTTGCG 
AGACGGAATG CAGAAGTTTT TCTCAAGTAT ATCCATCGCA ATAGCGTCAC CATGGCAAAT 
ATCTCTGGCC ATAGCATCAC TGTCTCTGGG GTCTCTGAGG TTACTGGCCA TTCCAGAGTC 
CCTGAACAAC AGGTCAAAGG TATCCTTAGT GAGCTGCTCC AGAGGGAAAA CAGAGTTCTT 
CATTTCTGGA CTTTAAAGAA ACGCAGATTG GATCAGTGCC AGCAGTATCT GATGTTCCAA 
ACCCACGCAC AGCAGGCTCT TGATTGGTTG CAGCAGTCAG GTGACCATTA CTTGGCCACA 
CATACGTCAC CAGGTGCAAC CATGGCTGAG ACACAGGAGC TGCTGAACCA ATACAGAGAG 
TTCTGCATTT CTGCAAAGGT GCACACACAG GAGAAGGTTC ACCTGCTGAT CCAGCTGGCT 
GAGAGTATGC TGGCTAAAGG TCACACCCAC CGCACTGAGC TCCGCCGCTG CGTTTCAACA 
GTGGATAAGC GCTACCGTGA CTTCTCTGTC AGGATGGGAC AATATCGCCA CCTGCTAGAG 
ACAGCGCTGG GAGGCTGTTC TCAGGACAAT AAAGACCTGG AGCTGGAGCT GATCCCCAAT 
AGTCTCTCGG ACTCTGACCC AGAGGTCAAC CTATCTGACC CAAGTCACCA AGTCAGTGAT 
GAGAAAAGAC GGTCCGCCCG CAAAAAGGAG TACATCATGG CAGAGCTCCT TCAGACAGAA 
CGAGTCTATG TCAGAGACCT GCAGGAGTGC ATAGAGACGT ACCTGTGGGA GATGACAAGC 
GGTTCAGAAG ATGTTCCACT CGGTCTTGCC AACAGTCACT ACACTGTGTT TGGAAACATT 
CAGGACATCT ACGAGTTCCA TAACAGTATT TTCTTAAAGG AGTTAGAGAA CTACGAGCAG 
CTTCCTGAAG ACGTGGGACA CTGTTTCGTC ACCTGGGCTG ATAAGTTCCA CATGTATGTG 
ACGTACTGCA GAAACAAACC AGACTCCAGT TTATTGATCC AACAGCATGG AGTGGGATTT 
TTTGAGGAGG TGCAGAGGAG ACACGGACTT GCTAACTCCA TCTCCTCTGC GCTGATTAAA 
CCGGTCCAGA GGATCACCAA GTACCAGCTG TTGCTTAAGG AGCTGCTGGC TTGCTGCGAG 
GAAGGCAAGG GGGAGATCAA GGAAGGCCTG GACGTGATGC TGAGTGTCCC AAAGAGAGCC 
AACGATGCAA TGCACGTCAG CATGTTAGAA GGTCTGGAGG ACGGGGTGGA GGTGCAGGGA 
GAGCTCCTCC TGCAGGACTC TTTCCTGGTC TGGGAGCCTA AGTCTCTGAT AAGAAAGGGG 
CGGGACAGAC ACTTATTCTT GTTTGAACTG TCACTCATCT TTAGCAAAGA GATTAAAGAC 
TCGTCCGGCC GCACCAAGTA TCTCTACAAA AGTCGCTTGA GGACGTCAGA GCTGGGAGTG 
ACGGAGCACA TAGAGGGGGA TCAGTGTAAG TTTGCACTGT GGGTGGGACG AACGCCGACA 
TCAGACAACA AGACCGTCCT GAAGGCTTCA TCCCTCGAGC TAAAGCAGGA GTGGGTTCGC 
AGTATCCGAC AGGTGATCCA GGAGAGGCGG GGTCACCTGC GAGGCGCACT GAGGGAGCCT 
ATCCCGCTCC CAAAAACCCC CAACCCCACG CTGGGACGAC AGCGCAGCAT CACCCGCAGG 
GAGACGAGCG AGGACGCAGA CAGCCTGGGT GATGCCAGCA GTCAGCCTGA CACGGTTTCC 
ATCGCATCAC GAACGTCGCA GAACACTGCC GACAGCGACA AGCTCTCTGG GGGCTGCGAG 
CTAGTGGTGG TGCTGCTGGA CTTCACCTCT GGAGGACCGG GAGAGCTCAG CGTCCGCTGC 
GGCCAGACGG TGGAGCTGGT GGAGCGCTCT GCAGACCGGC CTGGCTGGTG TCTGATCAGA 
ACCACGGATC AGACGCCCCA GCAAGAGGGT TTGCTGCCCA TGAGCGCCCT CTGCCTGTCG 
CACTCCCACA GTGCAGGTGA CATGGAGGGG CTCCTCCCAG CCTCCACCAC CTCCTCTAGC 
ACCTCCTCCA CCTGCACCAC CACCTCCTCC TCTCTCAACT CCTCCTCCAC CACCACCTCC 
TCATCCACCA TGAGCGCAGC AAAGGATTCC AGTCTGTTCA GTTCTGAGGG TTCAGGCCTC 
CCGACTCAGA CCAGCAGTCA AAGTGCCACC TCCCCCACCG TAGGTGGACC GGGCGGAGCT 
CCAGTAGGTG CAGTAGGGTC TCCGGGGCCC AAACGGAGCG GCTCTGGCAC AGGCAACACG 
TTAAAGCGGT GGTTGACCAG CCCTGTGCGA AGGCTGAGTC AGGGAAAAGC TGACGGACAG 
AAAAAACCTC CAATCAGAGC CAGGAGGCGG GACAACAAGC TCGAGCTGGC CACGCCCCTT 
ACCGTAAATA CACAGATGCA GAAGGCAAGA AAGGAGGAGG CGGGGCAAAG TGGAGGGGAG 
GAGGAGCAAG AGGAAGAAAG CCACACCCCA TTACCTCCAC CGATGCAGAT TATGAAAGAC 
CCCAACAATC CAGAGGCTCT GGACTCAGAT CAGGGATCAA ACGAGTCCGA TACTGAACAG 
AGAAACAAAG CACTAAGAGG ACGAATGTTT GTTGTTAATG AGATGGTCCA ATCAGAGAAG 
GACTACGTAA AGGACCTAGG AGTGATTGTT GAGGGCTTCA TGTCCAGGCT TGAGGTCAGG 
GGGATCCCAG AAGACATGAG GGGAAAAGAC AAGATCGTGT TTGGCAACAT CCAGCAGATC 
TACGACTGGC ACCGCGACTT CTTTCTGGTG GAGCTAGAGC GCTGCATCCA GAACCACGAT 
CTGCTGGCCG ACCTGTTCAT CAGACATGAG CGTCGTCTGC ACATGTATGT GGTTTACTGT 
CAAAACAAGC CCAAGTCGGA GTTCATCGTC ATTGAGTATG AGACCTTCTT TGAGGAGATC 
CAGCATGAGA TCAGCTGCAG GATGTCGATC AGTGATTATC TGATCAAACC CATCCAGAGA 
ATCACCAAGT ACCAGCTACT ACTGAAGGAC TTCTTCAAAT ATACGACCAA AGCAGGGCTG 
GACACTGAGG AGATTGAGAA AGCGGTGGAG CTGATGTCAT TGGTTCCAAA GCGCTGCAAC 
GACATGATGA ACCTGGGACG CCTGCAGGGA TATGAGGGGA AGTTGACGTC TCAGGGGAAG 
CTGCTGCAGC AGGAGACCTT CTGCGTTTGG GAGCAGGACG GGGGAGTCTT GTCTCGCAGC 
AAAGAGCGCA GAGTCTTCCT CTTCGAGCAG ATCATCATCT TCAGTGAACT GCTCCGGAAA 
GGCTCCAACA ATCCAGGATA CCAGTTCAAG AACAGCATCA AGGTGAGTTA CCTGGCCATG 
CAGGACAGCG TAGACGGCGA TCCCTGTAAG TTTGTGCTGT GGTCCCGGGG CTCGGCAGAG 
CGCTTCACTC TACAAGCGTC CTCCGCCAGC ATCAAGATGA CCTGGGTGGA GACCATTGCC 
ACTCTGCTGG ACGCCCAGAA CAACTTCCTC TCTGCTCTCC AGTCACCTAT TGAGTACCAG 
AGAAAAGAGG GCGGGATTTC AGTGACCCGT CCACTGTCGT CTGGTCGTCC ACCGTCTGCT 
CCGCCCACGC CTAACGGCCA TGCCACTCAA CCTCCTGCTG ACAGTGAGGA TGACCAGGAG 
CTGGTTCAGG TGCTGCAGGA CTTTGTGGCC GTGCGGGAGG ACGAGATTTC CGTGTTTCGG 
GGAGAGAAAG TCCAGATTCT GTCGTCCAAC CAGCAGGGTC AGAGTCTTGT TTACAGACCT 
GCCAACACCG ACTCGCCCGC CGCCGAGGGC TGGGTGCCAC GAAGCGTGCT CAAAACACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101166941 ( XM_011482977.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011481279.2 Oryzias latipes kalirin-like (LOC101166941), transcript variant X2, mRNA.

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CloneID	OJa74579
Clone ID Related Accession (Same CDS sequence)	XM_011482977.2 , XM_011482977.3
Accession Version	XM_011482977.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	kalirin-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.1) and transcript sequence (GFIO01025530.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011482977.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 506 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAATCCGG CAGATGGAGG AGGAGAAGCG GGCCCGGACG GCCAGGCGAC GGCAGGTTCT 
GGTAAAGGGG AGTCTGTCCG GGCGGTCGAT GTCTTGACGG TGCTCAGAGA AAAAGTGGCC 
TTTGTGTCGG GTGGACGCGA CAAACGAGGT GGACCAATTC TCACCTTTCC TGCTAGGACC 
AATCACGATC GAATAAAACA GGAAGACCTC AGCCGCTTGG TTACTTATTT ATCAACTATC 
CCTAGCGAGG ATGTGCGTGC CCGTGGCTTT ACCGTTGTGG TGGATATGCG GGGCAGTAAG 
TGGGACAGCG TGAAGCCGGT TCTGCGAACA CTACAGGAGT CCTTTTCCTC AAACATCCAC 
ACAGCACTAC TCATCAAACC GGACACCTTC TGGCAGAAAC ACAAGACCAA CCTAGGCAGT 
GCCAAGCTGA GTTTCGAGAC CAGTCTGGTG TCAGTGGAGG GTTTGTCCCG ACTTATTGAT 
CCCTCTCAGC TGACCGCTGA CCTCGGAGGT TCATTGGAGT ATGACCATGT GGAGTGGACT 
GAGCTGCGTC TTGGTTTAGA GGAGTTCTCA GGGGCTGCCA CACAGCTACT AACCCAACTG 
GAGCAGCTGG AAGCCGGGCT GAGCCGCATA CCCGAGCCTC AGGACATTGA TGAGGCCCGC 
AAACTCTTGG AAGAACACAC TCGCCTCCGC AATAGCATCG CCACTGCGCC TGTTGATGCC 
CTCGACCGAG AAGGGAAGTG TTTACTTCAG CGGATGCGCC TAGGTTCATC CCACGGTGAA 
GCCTCAAATG AGAGTGGGGG TGGTGGGGGA ACTTGGTTGT CAGGAGGAGC AGATTTCCAG 
AGCGTGGCGC CGAAGGTTTC GTCTTTATTG GACCGGCTGC AGGGGGCTCG CAGTCACTTA 
CTGCAGTGCT GGGGTGATAA GAAGCTGCAC TTGGACCAGG CACTGCAACT CCACCTGTTT 
GAACAGGATG CTACCAAGAT GTCAGAGTGG ATCGCTCACA GTAAAGAGCT CTTCATGCAG 
AGTCTGGCAG AAGTCGGTCA GAACCACCAG CACGCTCTCG ATCTACAAAC GCAGCACCAG 
CATTTCACCG CCAACTGCAT GAACGGCAGT GTGAATGTGG ACAGTGTCGT TACCGTGGCA 
GCAAGGCTCG CGGAGGCCGG CCACTACGGA GCAGAGCGGA TTGCTTTGGT GTCGTCAAAG 
TTACAGGACA ACTGGAAGTC AATGACAAGT GCTCTGGAAG AGCGGAGCAA CATGCTTGCC 
ATGGCAACCG GTTTCCATCA AGGGGCAGAG CAGTTTCTAG CCCGAGTGGA GGGATGGGTT 
AAACTGTGCT CTGAAGGGCC ATTGCCATCA ACAACAGCCG AGCTGGAACC TGCAATAAAA 
AAACACCAGG AGCTCAATGA GGAGATTTCA GCCAACTACA CACAGGTCAG TGAAAGCGGT 
AAGGCATTGC TGGAGGTCCT TCAGCGTTGC CCCACAACAG ATTCTGACGA ATCAGCAACC 
AAACCAGATT TCACTGCGGC CACACACACC ATTATGGGGG TTCTACACCA GGTGATGCAG 
GGTCATCACG AAGTAGAGGG AGCATGGCAG CATCGTAAGC TCCGCCTCCA CCAGCGCCTG 
CAGTTATGTG TGTTTCAACA GGATGTTAAA CAGGTTTTGG ACTGGGTGGA GCAGCATGGA 
GAGGTTTTCC TGAACAAACA CACGGGTGTG GGGAAGTCCT TGCACCGGGC ACGGGCGCTG 
CAGAAGCGAC ACGATGACTT TCAGCAGGTT GCACAGAATA CGTACACCAA TGCAGAGAAG 
CTTCTGGAGG CAGCAGACCA GTTGGCGCAG TCTGGAGAGT GTGAGGAAGA GGAGATCTAC 
CAGGCAGCCA GAGACCTGGA GCTGCGAATG CAGGCCTTCA TTCAGCGCGT TGAACAGAGG 
AAGCTGCTGC TGGACCTCGC CGTATCCTTC TACACGCACA CAAAGGAGCT CTCTGCATGG 
ATGGAGGGCC TCCAGAAGCA GCTCTCCTCA GACCTCCTCG TGTGCCCGGA CACTGTGGAA 
GCTCTGCAGG CTTTAATCAG CCAACAGCAG CAGCAGCAGA CCAACACTCA GGAAGCTGCG 
ATGAGTGTCA TCCGGGAAGG AGAAGACCTC ATCCTGCAAC TCAGGCCTCA GGGCAGCTCG 
GTGGCCCATG TGGAATCGGT TTTGCAGCAA CTGGAAGAGT CTCATGTTCA GCTTGAGGAA 
CTTTTTCACC AGAGGAAGAT CCGAGTGGAC ATTTTCCTGC AGTTCCGCAT ACTGCACCAG 
TGCACGCTGG AGGTTACAGC TGAGATGGAT GCTTGGAAAA TGGACTTGGA AAAACAATCC 
CAGGAATTTT CCTCGGAGAA GGTCGCCTCA AATCGGCTTG CTGAAACCAA CCAGGACAAG 
CTGACCCAGG ATAAGCTGGC ACTAGGACAG TCCCGGCTTG TGGAGTCCAG CCCGGAAGCG 
TTAACACTAG CACAGCAGCG GATTCACAGG CACATGGAAC GGAGGCTGGC GATGAACAAC 
ATGATATATG AGGTCATCCA GCAAGGTCAT GACCTTCAGC AGTACATCTC GGAGGTGCAG 
GCATCAGGTA TCGAGCTGTC AGACGTGGAG GGTGGGCTGT CCTCTCAGGT GGAGGAGCTG 
CAGCGCCTCC TGCAGGACAA ACAGAAGGAG CTGCAGCAGA TTGCCGATCA CACGCATACG 
CTGCTGGAGC AGAACCTGCA GCTGAGACAC CTCCAGAGCC AGGTCAAGCA GGTGCTCGGC 
TGGATCTCAG AGGGGGAGGT GATGCTGTCC TCCTGCATGT TGAATTCCAG CTGTCTGTCA 
GAGGCGGAGC AGCTGCAGAA GGAACACGAG CGCCTCCAAC AGGCCATAGA GGCTCTGCTG 
CACGCAGACT CCCTGCAGAG GACTCATCAG GTGGCGCTGG CCCTGCAGCA GAGAGCGGAG 
CTGCTGGTCA GGTCTGGCCA CTATGATCCA GATGCAGTGA GGTCCTGCGC TCAGACTGTA 
GCGCTGCATT GGCAGACACT AATGCTCAGG ATTGAAGACA GACTCAAACT GGTCAATGCG 
TCGGCTGCTT TCTACAGGAC ATCCCAGCAG GTATGTGGGG TCCTGGAGAG TCTGGAGCAG 
GAATACCGCA GGGAGGAGGA CTGGTGTGCA GGAGGAGAGA GGCAACCAGA GCAACTGCTG 
CCGCTAATCA ATAAACACAT GGAGCAGAAG GAGGCTTTTC TGAAGGCCTG TACTCTTGCG 
AGACGGAATG CAGAAGTTTT TCTCAAGTAT ATCCATCGCA ATAGCGTCAC CATGGCAAAT 
ATCTCTGGCC ATAGCATCAC TGTCTCTGGG GTCTCTGAGG TTACTGGCCA TTCCAGAGTC 
CCTGAACAAC AGGTCAAAGG TATCCTTAGT GAGCTGCTCC AGAGGGAAAA CAGAGTTCTT 
CATTTCTGGA CTTTAAAGAA ACGCAGATTG GATCAGTGCC AGCAGTATCT GATGTTCCAA 
ACCCACGCAC AGCAGGCTCT TGATTGGTTG CAGCAGTCAG GTGACCATTA CTTGGCCACA 
CATACGTCAC CAGGTGCAAC CATGGCTGAG ACACAGGAGC TGCTGAACCA ATACAGAGAG 
TTCTGCATTT CTGCAAAGCA CACACAGGAG AAGGTTCACC TGCTGATCCA GCTGGCTGAG 
AGTATGCTGG CTAAAGGTCA CACCCACCGC ACTGAGCTCC GCCGCTGCGT TTCAACAGTG 
GATAAGCGCT ACCGTGACTT CTCTGTCAGG ATGGGACAAT ATCGCCACCT GCTAGAGACA 
GCGCTGGGAG GCTGTTCTCA GGACAATAAA GACCTGGAGC TGGAGCTGAT CCCCAATAGT 
CTCTCGGACT CTGACCCAGA GGTCAACCTA TCTGACCCAA GTCACCAAGT CAGTGATGAG 
AAAAGACGGT CCGCCCGCAA AAAGGAGTAC ATCATGGCAG AGCTCCTTCA GACAGAACGA 
GTCTATGTCA GAGACCTGCA GGAGTGCATA GAGACGTACC TGTGGGAGAT GACAAGCGGT 
TCAGAAGATG TTCCACTCGG TCTTGCCAAC AGTCACTACA CTGTGTTTGG AAACATTCAG 
GACATCTACG AGTTCCATAA CAGTATTTTC TTAAAGGAGT TAGAGAACTA CGAGCAGCTT 
CCTGAAGACG TGGGACACTG TTTCGTCACC TGGGCTGATA AGTTCCACAT GTATGTGACG 
TACTGCAGAA ACAAACCAGA CTCCAGTTTA TTGATCCAAC AGCATGGAGT GGGATTTTTT 
GAGGAGGTGC AGAGGAGACA CGGACTTGCT AACTCCATCT CCTCTGCGCT GATTAAACCG 
GTCCAGAGGA TCACCAAGTA CCAGCTGTTG CTTAAGGAGC TGCTGGCTTG CTGCGAGGAA 
GGCAAGGGGG AGATCAAGGA AGGCCTGGAC GTGATGCTGA GTGTCCCAAA GAGAGCCAAC 
GATGCAATGC ACGTCAGCAT GTTAGAAGGT CTGGAGGACG GGGTGGAGGT GCAGGGAGAG 
CTCCTCCTGC AGGACTCTTT CCTGGTCTGG GAGCCTAAGT CTCTGATAAG AAAGGGGCGG 
GACAGACACT TATTCTTGTT TGAACTGTCA CTCATCTTTA GCAAAGAGAT TAAAGACTCG 
TCCGGCCGCA CCAAGTATCT CTACAAAAGT CGCTTGAGGA CGTCAGAGCT GGGAGTGACG 
GAGCACATAG AGGGGGATCA GTGTAAGTTT GCACTGTGGG TGGGACGAAC GCCGACATCA 
GACAACAAGA CCGTCCTGAA GGCTTCATCC CTCGAGCTAA AGCAGGAGTG GGTTCGCAGT 
ATCCGACAGG TGATCCAGGA GAGGCGGGGT CACCTGCGAG GCGCACTGAG GGAGCCTATC 
CCGCTCCCAA AAACCCCCAA CCCCACGCTG GGACGACAGC GCAGCATCAC CCGCAGGGAG 
ACGAGCGAGG ACGCAGACAG CCTGGGTGAT GCCAGCAGTC AGCCTGACAC GGTTTCCATC 
GCATCACGAA CGTCGCAGAA CACTGCCGAC AGCGACAAGC TCTCTGGGGG CTGCGAGCTA 
GTGGTGGTGC TGCTGGACTT CACCTCTGGA GGACCGGGAG AGCTCAGCGT CCGCTGCGGC 
CAGACGGTGG AGCTGGTGGA GCGCTCTGCA GACCGGCCTG GCTGGTGTCT GATCAGAACC 
ACGGATCAGA CGCCCCAGCA AGAGGGTTTG CTGCCCATGA GCGCCCTCTG CCTGTCGCAC 
TCCCACAGTG CAGGTGACAT GGAGGGGCTC CTCCCAGCCT CCACCACCTC CTCTAGCACC 
TCCTCCACCT GCACCACCAC CTCCTCCTCT CTCAACTCCT CCTCCACCAC CACCTCCTCA 
TCCACCATGA GCGCAGCAAA GGATTCCAGT CTGTTCAGTT CTGAGGGTTC AGGCCTCCCG 
ACTCAGACCA GCAGTCAAAG TGCCACCTCC CCCACCGTAG GTGGACCGGG CGGAGCTCCA 
GTAGGTGCAG TAGGGTCTCC GGGGCCCAAA CGGAGCGGCT CTGGCACAGG CAACACGTTA 
AAGCGGTGGT TGACCAGCCC TGTGCGAAGG CTGAGTCAGG GAAAAGCTGA CGGACAGAAA 
AAACCTCCAA TCAGAGCCAG GAGGCGGGAC AACAAGCTCG AGCTGGCCAC GCCCCTTACC 
GTAAATACAC AGATGCAGAA GGCAAGAAAG GAGGAGGCGG GGCAAAGTGG AGGGGAGGAG 
GAGCAAGAGG AAGAAAGCCA CACCCCATTA CCTCCACCGA TGCAGATTAT GAAAGACCCC 
AACAATCCAG AGGCTCTGGA CTCAGATCAG GGATCAAACG AGTCCGATAC TGAACAGAGA 
AACAAAGCAC TAAGAGGACG AATGTTTGTT GTTAATGAGA TGGTCCAATC AGAGAAGGAC 
TACGTAAAGG ACCTAGGAGT GATTGTTGAG GGCTTCATGT CCAGGCTTGA GGTCAGGGGG 
ATCCCAGAAG ACATGAGGGG AAAAGACAAG ATCGTGTTTG GCAACATCCA GCAGATCTAC 
GACTGGCACC GCGACTTCTT TCTGGTGGAG CTAGAGCGCT GCATCCAGAA CCACGATCTG 
CTGGCCGACC TGTTCATCAG ACATGAGCGT CGTCTGCACA TGTATGTGGT TTACTGTCAA 
AACAAGCCCA AGTCGGAGTT CATCGTCATT GAGTATGAGA CCTTCTTTGA GGAGATCCAG 
CATGAGATCA GCTGCAGGAT GTCGATCAGT GATTATCTGA TCAAACCCAT CCAGAGAATC 
ACCAAGTACC AGCTACTACT GAAGGACTTC TTCAAATATA CGACCAAAGC AGGGCTGGAC 
ACTGAGGAGA TTGAGAAAGC GGTGGAGCTG ATGTCATTGG TTCCAAAGCG CTGCAACGAC 
ATGATGAACC TGGGACGCCT GCAGGGATAT GAGGGGAAGT TGACGTCTCA GGGGAAGCTG 
CTGCAGCAGG AGACCTTCTG CGTTTGGGAG CAGGACGGGG GAGTCTTGTC TCGCAGCAAA 
GAGCGCAGAG TCTTCCTCTT CGAGCAGATC ATCATCTTCA GTGAACTGCT CCGGAAAGGC 
TCCAACAATC CAGGATACCA GTTCAAGAAC AGCATCAAGG TGAGTTACCT GGCCATGCAG 
GACAGCGTAG ACGGCGATCC CTGTAAGTTT GTGCTGTGGT CCCGGGGCTC GGCAGAGCGC 
TTCACTCTAC AAGCGTCCTC CGCCAGCATC AAGATGACCT GGGTGGAGAC CATTGCCACT 
CTGCTGGACG CCCAGAACAA CTTCCTCTCT GCTCTCCAGT CACCTATTGA GTACCAGAGA 
AAAGAGGGCG GGATTTCAGT GACCCGTCCA CTGTCGTCTG GTCGTCCACC GTCTGCTCCG 
CCCACGCCTA ACGGCCATGC CACTCAACCT CCTGCTGACA GTGAGGATGA CCAGGAGCTG 
GTTCAGGTGC TGCAGGACTT TGTGGCCGTG CGGGAGGACG AGATTTCCGT GTTTCGGGGA 
GAGAAAGTCC AGATTCTGTC GTCCAACCAG CAGGGTCAGA GTCTTGTTTA CAGACCTGCC 
AACACCGACT CGCCCGCCGC CGAGGGCTGG GTGCCACGAA GCGTGCTCAA AACACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011481279.2
CDS	452..7531
Translation	MNPADGGGEAGPDGQATAGSGKGESVRAVDVLTVLREKVAFVSGGRDKRGGPILTFPARTNHDRIKQEDLSRLVTYLSTIPSEDVRARGFTVVVDMRGSKWDSVKPVLRTLQESFSSNIHTALLIKPDTFWQKHKTNLGSAKLSFETSLVSVEGLSRLIDPSQLTADLGGSLEYDHVEWTELRLGLEEFSGAATQLLTQLEQLEAGLSRIPEPQDIDEARKLLEEHTRLRNSIATAPVDALDREGKCLLQRMRLGSSHGEASNESGGGGGTWLSGGADFQSVAPKVSSLLDRLQGARSHLLQCWGDKKLHLDQALQLHLFEQDATKMSEWIAHSKELFMQSLAEVGQNHQHALDLQTQHQHFTANCMNGSVNVDSVVTVAARLAEAGHYGAERIALVSSKLQDNWKSMTSALEERSNMLAMATGFHQGAEQFLARVEGWVKLCSEGPLPSTTAELEPAIKKHQELNEEISANYTQVSESGKALLEVLQRCPTTDSDESATKPDFTAATHTIMGVLHQVMQGHHEVEGAWQHRKLRLHQRLQLCVFQQDVKQVLDWVEQHGEVFLNKHTGVGKSLHRARALQKRHDDFQQVAQNTYTNAEKLLEAADQLAQSGECEEEEIYQAARDLELRMQAFIQRVEQRKLLLDLAVSFYTHTKELSAWMEGLQKQLSSDLLVCPDTVEALQALISQQQQQQTNTQEAAMSVIREGEDLILQLRPQGSSVAHVESVLQQLEESHVQLEELFHQRKIRVDIFLQFRILHQCTLEVTAEMDAWKMDLEKQSQEFSSEKVASNRLAETNQDKLTQDKLALGQSRLVESSPEALTLAQQRIHRHMERRLAMNNMIYEVIQQGHDLQQYISEVQASGIELSDVEGGLSSQVEELQRLLQDKQKELQQIADHTHTLLEQNLQLRHLQSQVKQVLGWISEGEVMLSSCMLNSSCLSEAEQLQKEHERLQQAIEALLHADSLQRTHQVALALQQRAELLVRSGHYDPDAVRSCAQTVALHWQTLMLRIEDRLKLVNASAAFYRTSQQVCGVLESLEQEYRREEDWCAGGERQPEQLLPLINKHMEQKEAFLKACTLARRNAEVFLKYIHRNSVTMANISGHSITVSGVSEVTGHSRVPEQQVKGILSELLQRENRVLHFWTLKKRRLDQCQQYLMFQTHAQQALDWLQQSGDHYLATHTSPGATMAETQELLNQYREFCISAKHTQEKVHLLIQLAESMLAKGHTHRTELRRCVSTVDKRYRDFSVRMGQYRHLLETALGGCSQDNKDLELELIPNSLSDSDPEVNLSDPSHQVSDEKRRSARKKEYIMAELLQTERVYVRDLQECIETYLWEMTSGSEDVPLGLANSHYTVFGNIQDIYEFHNSIFLKELENYEQLPEDVGHCFVTWADKFHMYVTYCRNKPDSSLLIQQHGVGFFEEVQRRHGLANSISSALIKPVQRITKYQLLLKELLACCEEGKGEIKEGLDVMLSVPKRANDAMHVSMLEGLEDGVEVQGELLLQDSFLVWEPKSLIRKGRDRHLFLFELSLIFSKEIKDSSGRTKYLYKSRLRTSELGVTEHIEGDQCKFALWVGRTPTSDNKTVLKASSLELKQEWVRSIRQVIQERRGHLRGALREPIPLPKTPNPTLGRQRSITRRETSEDADSLGDASSQPDTVSIASRTSQNTADSDKLSGGCELVVVLLDFTSGGPGELSVRCGQTVELVERSADRPGWCLIRTTDQTPQQEGLLPMSALCLSHSHSAGDMEGLLPASTTSSSTSSTCTTTSSSLNSSSTTTSSSTMSAAKDSSLFSSEGSGLPTQTSSQSATSPTVGGPGGAPVGAVGSPGPKRSGSGTGNTLKRWLTSPVRRLSQGKADGQKKPPIRARRRDNKLELATPLTVNTQMQKARKEEAGQSGGEEEQEEESHTPLPPPMQIMKDPNNPEALDSDQGSNESDTEQRNKALRGRMFVVNEMVQSEKDYVKDLGVIVEGFMSRLEVRGIPEDMRGKDKIVFGNIQQIYDWHRDFFLVELERCIQNHDLLADLFIRHERRLHMYVVYCQNKPKSEFIVIEYETFFEEIQHEISCRMSISDYLIKPIQRITKYQLLLKDFFKYTTKAGLDTEEIEKAVELMSLVPKRCNDMMNLGRLQGYEGKLTSQGKLLQQETFCVWEQDGGVLSRSKERRVFLFEQIIIFSELLRKGSNNPGYQFKNSIKVSYLAMQDSVDGDPCKFVLWSRGSAERFTLQASSASIKMTWVETIATLLDAQNNFLSALQSPIEYQRKEGGISVTRPLSSGRPPSAPPTPNGHATQPPADSEDDQELVQVLQDFVAVREDEISVFRGEKVQILSSNQQGQSLVYRPANTDSPAAEGWVPRSVLKTH

Target ORF information:

RefSeq Version	XM_011482977.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes kalirin-like (LOC101166941), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011482977.2

ORF Insert Sequence:

ATGAATCCGG CAGATGGAGG AGGAGAAGCG GGCCCGGACG GCCAGGCGAC GGCAGGTTCT 
GGTAAAGGGG AGTCTGTCCG GGCGGTCGAT GTCTTGACGG TGCTCAGAGA AAAAGTGGCC 
TTTGTGTCGG GTGGACGCGA CAAACGAGGT GGACCAATTC TCACCTTTCC TGCTAGGACC 
AATCACGATC GAATAAAACA GGAAGACCTC AGCCGCTTGG TTACTTATTT ATCAACTATC 
CCTAGCGAGG ATGTGCGTGC CCGTGGCTTT ACCGTTGTGG TGGATATGCG GGGCAGTAAG 
TGGGACAGCG TGAAGCCGGT TCTGCGAACA CTACAGGAGT CCTTTTCCTC AAACATCCAC 
ACAGCACTAC TCATCAAACC GGACACCTTC TGGCAGAAAC ACAAGACCAA CCTAGGCAGT 
GCCAAGCTGA GTTTCGAGAC CAGTCTGGTG TCAGTGGAGG GTTTGTCCCG ACTTATTGAT 
CCCTCTCAGC TGACCGCTGA CCTCGGAGGT TCATTGGAGT ATGACCATGT GGAGTGGACT 
GAGCTGCGTC TTGGTTTAGA GGAGTTCTCA GGGGCTGCCA CACAGCTACT AACCCAACTG 
GAGCAGCTGG AAGCCGGGCT GAGCCGCATA CCCGAGCCTC AGGACATTGA TGAGGCCCGC 
AAACTCTTGG AAGAACACAC TCGCCTCCGC AATAGCATCG CCACTGCGCC TGTTGATGCC 
CTCGACCGAG AAGGGAAGTG TTTACTTCAG CGGATGCGCC TAGGTTCATC CCACGGTGAA 
GCCTCAAATG AGAGTGGGGG TGGTGGGGGA ACTTGGTTGT CAGGAGGAGC AGATTTCCAG 
AGCGTGGCGC CGAAGGTTTC GTCTTTATTG GACCGGCTGC AGGGGGCTCG CAGTCACTTA 
CTGCAGTGCT GGGGTGATAA GAAGCTGCAC TTGGACCAGG CACTGCAACT CCACCTGTTT 
GAACAGGATG CTACCAAGAT GTCAGAGTGG ATCGCTCACA GTAAAGAGCT CTTCATGCAG 
AGTCTGGCAG AAGTCGGTCA GAACCACCAG CACGCTCTCG ATCTACAAAC GCAGCACCAG 
CATTTCACCG CCAACTGCAT GAACGGCAGT GTGAATGTGG ACAGTGTCGT TACCGTGGCA 
GCAAGGCTCG CGGAGGCCGG CCACTACGGA GCAGAGCGGA TTGCTTTGGT GTCGTCAAAG 
TTACAGGACA ACTGGAAGTC AATGACAAGT GCTCTGGAAG AGCGGAGCAA CATGCTTGCC 
ATGGCAACCG GTTTCCATCA AGGGGCAGAG CAGTTTCTAG CCCGAGTGGA GGGATGGGTT 
AAACTGTGCT CTGAAGGGCC ATTGCCATCA ACAACAGCCG AGCTGGAACC TGCAATAAAA 
AAACACCAGG AGCTCAATGA GGAGATTTCA GCCAACTACA CACAGGTCAG TGAAAGCGGT 
AAGGCATTGC TGGAGGTCCT TCAGCGTTGC CCCACAACAG ATTCTGACGA ATCAGCAACC 
AAACCAGATT TCACTGCGGC CACACACACC ATTATGGGGG TTCTACACCA GGTGATGCAG 
GGTCATCACG AAGTAGAGGG AGCATGGCAG CATCGTAAGC TCCGCCTCCA CCAGCGCCTG 
CAGTTATGTG TGTTTCAACA GGATGTTAAA CAGGTTTTGG ACTGGGTGGA GCAGCATGGA 
GAGGTTTTCC TGAACAAACA CACGGGTGTG GGGAAGTCCT TGCACCGGGC ACGGGCGCTG 
CAGAAGCGAC ACGATGACTT TCAGCAGGTT GCACAGAATA CGTACACCAA TGCAGAGAAG 
CTTCTGGAGG CAGCAGACCA GTTGGCGCAG TCTGGAGAGT GTGAGGAAGA GGAGATCTAC 
CAGGCAGCCA GAGACCTGGA GCTGCGAATG CAGGCCTTCA TTCAGCGCGT TGAACAGAGG 
AAGCTGCTGC TGGACCTCGC CGTATCCTTC TACACGCACA CAAAGGAGCT CTCTGCATGG 
ATGGAGGGCC TCCAGAAGCA GCTCTCCTCA GACCTCCTCG TGTGCCCGGA CACTGTGGAA 
GCTCTGCAGG CTTTAATCAG CCAACAGCAG CAGCAGCAGA CCAACACTCA GGAAGCTGCG 
ATGAGTGTCA TCCGGGAAGG AGAAGACCTC ATCCTGCAAC TCAGGCCTCA GGGCAGCTCG 
GTGGCCCATG TGGAATCGGT TTTGCAGCAA CTGGAAGAGT CTCATGTTCA GCTTGAGGAA 
CTTTTTCACC AGAGGAAGAT CCGAGTGGAC ATTTTCCTGC AGTTCCGCAT ACTGCACCAG 
TGCACGCTGG AGGTTACAGC TGAGATGGAT GCTTGGAAAA TGGACTTGGA AAAACAATCC 
CAGGAATTTT CCTCGGAGAA GGTCGCCTCA AATCGGCTTG CTGAAACCAA CCAGGACAAG 
CTGACCCAGG ATAAGCTGGC ACTAGGACAG TCCCGGCTTG TGGAGTCCAG CCCGGAAGCG 
TTAACACTAG CACAGCAGCG GATTCACAGG CACATGGAAC GGAGGCTGGC GATGAACAAC 
ATGATATATG AGGTCATCCA GCAAGGTCAT GACCTTCAGC AGTACATCTC GGAGGTGCAG 
GCATCAGGTA TCGAGCTGTC AGACGTGGAG GGTGGGCTGT CCTCTCAGGT GGAGGAGCTG 
CAGCGCCTCC TGCAGGACAA ACAGAAGGAG CTGCAGCAGA TTGCCGATCA CACGCATACG 
CTGCTGGAGC AGAACCTGCA GCTGAGACAC CTCCAGAGCC AGGTCAAGCA GGTGCTCGGC 
TGGATCTCAG AGGGGGAGGT GATGCTGTCC TCCTGCATGT TGAATTCCAG CTGTCTGTCA 
GAGGCGGAGC AGCTGCAGAA GGAACACGAG CGCCTCCAAC AGGCCATAGA GGCTCTGCTG 
CACGCAGACT CCCTGCAGAG GACTCATCAG GTGGCGCTGG CCCTGCAGCA GAGAGCGGAG 
CTGCTGGTCA GGTCTGGCCA CTATGATCCA GATGCAGTGA GGTCCTGCGC TCAGACTGTA 
GCGCTGCATT GGCAGACACT AATGCTCAGG ATTGAAGACA GACTCAAACT GGTCAATGCG 
TCGGCTGCTT TCTACAGGAC ATCCCAGCAG GTATGTGGGG TCCTGGAGAG TCTGGAGCAG 
GAATACCGCA GGGAGGAGGA CTGGTGTGCA GGAGGAGAGA GGCAACCAGA GCAACTGCTG 
CCGCTAATCA ATAAACACAT GGAGCAGAAG GAGGCTTTTC TGAAGGCCTG TACTCTTGCG 
AGACGGAATG CAGAAGTTTT TCTCAAGTAT ATCCATCGCA ATAGCGTCAC CATGGCAAAT 
ATCTCTGGCC ATAGCATCAC TGTCTCTGGG GTCTCTGAGG TTACTGGCCA TTCCAGAGTC 
CCTGAACAAC AGGTCAAAGG TATCCTTAGT GAGCTGCTCC AGAGGGAAAA CAGAGTTCTT 
CATTTCTGGA CTTTAAAGAA ACGCAGATTG GATCAGTGCC AGCAGTATCT GATGTTCCAA 
ACCCACGCAC AGCAGGCTCT TGATTGGTTG CAGCAGTCAG GTGACCATTA CTTGGCCACA 
CATACGTCAC CAGGTGCAAC CATGGCTGAG ACACAGGAGC TGCTGAACCA ATACAGAGAG 
TTCTGCATTT CTGCAAAGCA CACACAGGAG AAGGTTCACC TGCTGATCCA GCTGGCTGAG 
AGTATGCTGG CTAAAGGTCA CACCCACCGC ACTGAGCTCC GCCGCTGCGT TTCAACAGTG 
GATAAGCGCT ACCGTGACTT CTCTGTCAGG ATGGGACAAT ATCGCCACCT GCTAGAGACA 
GCGCTGGGAG GCTGTTCTCA GGACAATAAA GACCTGGAGC TGGAGCTGAT CCCCAATAGT 
CTCTCGGACT CTGACCCAGA GGTCAACCTA TCTGACCCAA GTCACCAAGT CAGTGATGAG 
AAAAGACGGT CCGCCCGCAA AAAGGAGTAC ATCATGGCAG AGCTCCTTCA GACAGAACGA 
GTCTATGTCA GAGACCTGCA GGAGTGCATA GAGACGTACC TGTGGGAGAT GACAAGCGGT 
TCAGAAGATG TTCCACTCGG TCTTGCCAAC AGTCACTACA CTGTGTTTGG AAACATTCAG 
GACATCTACG AGTTCCATAA CAGTATTTTC TTAAAGGAGT TAGAGAACTA CGAGCAGCTT 
CCTGAAGACG TGGGACACTG TTTCGTCACC TGGGCTGATA AGTTCCACAT GTATGTGACG 
TACTGCAGAA ACAAACCAGA CTCCAGTTTA TTGATCCAAC AGCATGGAGT GGGATTTTTT 
GAGGAGGTGC AGAGGAGACA CGGACTTGCT AACTCCATCT CCTCTGCGCT GATTAAACCG 
GTCCAGAGGA TCACCAAGTA CCAGCTGTTG CTTAAGGAGC TGCTGGCTTG CTGCGAGGAA 
GGCAAGGGGG AGATCAAGGA AGGCCTGGAC GTGATGCTGA GTGTCCCAAA GAGAGCCAAC 
GATGCAATGC ACGTCAGCAT GTTAGAAGGT CTGGAGGACG GGGTGGAGGT GCAGGGAGAG 
CTCCTCCTGC AGGACTCTTT CCTGGTCTGG GAGCCTAAGT CTCTGATAAG AAAGGGGCGG 
GACAGACACT TATTCTTGTT TGAACTGTCA CTCATCTTTA GCAAAGAGAT TAAAGACTCG 
TCCGGCCGCA CCAAGTATCT CTACAAAAGT CGCTTGAGGA CGTCAGAGCT GGGAGTGACG 
GAGCACATAG AGGGGGATCA GTGTAAGTTT GCACTGTGGG TGGGACGAAC GCCGACATCA 
GACAACAAGA CCGTCCTGAA GGCTTCATCC CTCGAGCTAA AGCAGGAGTG GGTTCGCAGT 
ATCCGACAGG TGATCCAGGA GAGGCGGGGT CACCTGCGAG GCGCACTGAG GGAGCCTATC 
CCGCTCCCAA AAACCCCCAA CCCCACGCTG GGACGACAGC GCAGCATCAC CCGCAGGGAG 
ACGAGCGAGG ACGCAGACAG CCTGGGTGAT GCCAGCAGTC AGCCTGACAC GGTTTCCATC 
GCATCACGAA CGTCGCAGAA CACTGCCGAC AGCGACAAGC TCTCTGGGGG CTGCGAGCTA 
GTGGTGGTGC TGCTGGACTT CACCTCTGGA GGACCGGGAG AGCTCAGCGT CCGCTGCGGC 
CAGACGGTGG AGCTGGTGGA GCGCTCTGCA GACCGGCCTG GCTGGTGTCT GATCAGAACC 
ACGGATCAGA CGCCCCAGCA AGAGGGTTTG CTGCCCATGA GCGCCCTCTG CCTGTCGCAC 
TCCCACAGTG CAGGTGACAT GGAGGGGCTC CTCCCAGCCT CCACCACCTC CTCTAGCACC 
TCCTCCACCT GCACCACCAC CTCCTCCTCT CTCAACTCCT CCTCCACCAC CACCTCCTCA 
TCCACCATGA GCGCAGCAAA GGATTCCAGT CTGTTCAGTT CTGAGGGTTC AGGCCTCCCG 
ACTCAGACCA GCAGTCAAAG TGCCACCTCC CCCACCGTAG GTGGACCGGG CGGAGCTCCA 
GTAGGTGCAG TAGGGTCTCC GGGGCCCAAA CGGAGCGGCT CTGGCACAGG CAACACGTTA 
AAGCGGTGGT TGACCAGCCC TGTGCGAAGG CTGAGTCAGG GAAAAGCTGA CGGACAGAAA 
AAACCTCCAA TCAGAGCCAG GAGGCGGGAC AACAAGCTCG AGCTGGCCAC GCCCCTTACC 
GTAAATACAC AGATGCAGAA GGCAAGAAAG GAGGAGGCGG GGCAAAGTGG AGGGGAGGAG 
GAGCAAGAGG AAGAAAGCCA CACCCCATTA CCTCCACCGA TGCAGATTAT GAAAGACCCC 
AACAATCCAG AGGCTCTGGA CTCAGATCAG GGATCAAACG AGTCCGATAC TGAACAGAGA 
AACAAAGCAC TAAGAGGACG AATGTTTGTT GTTAATGAGA TGGTCCAATC AGAGAAGGAC 
TACGTAAAGG ACCTAGGAGT GATTGTTGAG GGCTTCATGT CCAGGCTTGA GGTCAGGGGG 
ATCCCAGAAG ACATGAGGGG AAAAGACAAG ATCGTGTTTG GCAACATCCA GCAGATCTAC 
GACTGGCACC GCGACTTCTT TCTGGTGGAG CTAGAGCGCT GCATCCAGAA CCACGATCTG 
CTGGCCGACC TGTTCATCAG ACATGAGCGT CGTCTGCACA TGTATGTGGT TTACTGTCAA 
AACAAGCCCA AGTCGGAGTT CATCGTCATT GAGTATGAGA CCTTCTTTGA GGAGATCCAG 
CATGAGATCA GCTGCAGGAT GTCGATCAGT GATTATCTGA TCAAACCCAT CCAGAGAATC 
ACCAAGTACC AGCTACTACT GAAGGACTTC TTCAAATATA CGACCAAAGC AGGGCTGGAC 
ACTGAGGAGA TTGAGAAAGC GGTGGAGCTG ATGTCATTGG TTCCAAAGCG CTGCAACGAC 
ATGATGAACC TGGGACGCCT GCAGGGATAT GAGGGGAAGT TGACGTCTCA GGGGAAGCTG 
CTGCAGCAGG AGACCTTCTG CGTTTGGGAG CAGGACGGGG GAGTCTTGTC TCGCAGCAAA 
GAGCGCAGAG TCTTCCTCTT CGAGCAGATC ATCATCTTCA GTGAACTGCT CCGGAAAGGC 
TCCAACAATC CAGGATACCA GTTCAAGAAC AGCATCAAGG TGAGTTACCT GGCCATGCAG 
GACAGCGTAG ACGGCGATCC CTGTAAGTTT GTGCTGTGGT CCCGGGGCTC GGCAGAGCGC 
TTCACTCTAC AAGCGTCCTC CGCCAGCATC AAGATGACCT GGGTGGAGAC CATTGCCACT 
CTGCTGGACG CCCAGAACAA CTTCCTCTCT GCTCTCCAGT CACCTATTGA GTACCAGAGA 
AAAGAGGGCG GGATTTCAGT GACCCGTCCA CTGTCGTCTG GTCGTCCACC GTCTGCTCCG 
CCCACGCCTA ACGGCCATGC CACTCAACCT CCTGCTGACA GTGAGGATGA CCAGGAGCTG 
GTTCAGGTGC TGCAGGACTT TGTGGCCGTG CGGGAGGACG AGATTTCCGT GTTTCGGGGA 
GAGAAAGTCC AGATTCTGTC GTCCAACCAG CAGGGTCAGA GTCTTGTTTA CAGACCTGCC 
AACACCGACT CGCCCGCCGC CGAGGGCTGG GTGCCACGAA GCGTGCTCAA AACACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101166941 ( XM_011482977.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011481279.2 Oryzias latipes kalirin (LOC101166941), transcript variant X1, mRNA.

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CloneID	OJa74579
Clone ID Related Accession (Same CDS sequence)	XM_011482977.2 , XM_011482977.3
Accession Version	XM_011482977.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	kalirin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_011482977.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCGG CAGATGGAGG AGGAGAAGCG GGCCCGGACG GCCAGGCGAC GGCAGGTTCT 
GGTAAAGGGG AGTCTGTCCG GGCGGTCGAT GTCTTGACGG TGCTCAGAGA AAAAGTGGCC 
TTTGTGTCGG GTGGACGCGA CAAACGAGGT GGACCAATTC TCACCTTTCC TGCTAGGACC 
AATCACGATC GAATAAAACA GGAAGACCTC AGCCGCTTGG TTACTTATTT ATCAACTATC 
CCTAGCGAGG ATGTGCGTGC CCGTGGCTTT ACCGTTGTGG TGGATATGCG GGGCAGTAAG 
TGGGACAGCG TGAAGCCGGT TCTGCGAACA CTACAGGAGT CCTTTTCCTC AAACATCCAC 
ACAGCACTAC TCATCAAACC GGACACCTTC TGGCAGAAAC ACAAGACCAA CCTAGGCAGT 
GCCAAGCTGA GTTTCGAGAC CAGTCTGGTG TCAGTGGAGG GTTTGTCCCG ACTTATTGAT 
CCCTCTCAGC TGACCGCTGA CCTCGGAGGT TCATTGGAGT ATGACCATGT GGAGTGGACT 
GAGCTGCGTC TTGGTTTAGA GGAGTTCTCA GGGGCTGCCA CACAGCTACT AACCCAACTG 
GAGCAGCTGG AAGCCGGGCT GAGCCGCATA CCCGAGCCTC AGGACATTGA TGAGGCCCGC 
AAACTCTTGG AAGAACACAC TCGCCTCCGC AATAGCATCG CCACTGCGCC TGTTGATGCC 
CTCGACCGAG AAGGGAAGTG TTTACTTCAG CGGATGCGCC TAGGTTCATC CCACGGTGAA 
GCCTCAAATG AGAGTGGGGG TGGTGGGGGA ACTTGGTTGT CAGGAGGAGC AGATTTCCAG 
AGCGTGGCGC CGAAGGTTTC GTCTTTATTG GACCGGCTGC AGGGGGCTCG CAGTCACTTA 
CTGCAGTGCT GGGGTGATAA GAAGCTGCAC TTGGACCAGG CACTGCAACT CCACCTGTTT 
GAACAGGATG CTACCAAGAT GTCAGAGTGG ATCGCTCACA GTAAAGAGCT CTTCATGCAG 
AGTCTGGCAG AAGTCGGTCA GAACCACCAG CACGCTCTCG ATCTACAAAC GCAGCACCAG 
CATTTCACCG CCAACTGCAT GAACGGCAGT GTGAATGTGG ACAGTGTCGT TACCGTGGCA 
GCAAGGCTCG CGGAGGCCGG CCACTACGGA GCAGAGCGGA TTGCTTTGGT GTCGTCAAAG 
TTACAGGACA ACTGGAAGTC AATGACAAGT GCTCTGGAAG AGCGGAGCAA CATGCTTGCC 
ATGGCAACCG GTTTCCATCA AGGGGCAGAG CAGTTTCTAG CCCGAGTGGA GGGATGGGTT 
AAACTGTGCT CTGAAGGGCC ATTGCCATCA ACAACAGCCG AGCTGGAACC TGCAATAAAA 
AAACACCAGG AGCTCAATGA GGAGATTTCA GCCAACTACA CACAGGTCAG TGAAAGCGGT 
AAGGCATTGC TGGAGGTCCT TCAGCGTTGC CCCACAACAG ATTCTGACGA ATCAGCAACC 
AAACCAGATT TCACTGCGGC CACACACACC ATTATGGGGG TTCTACACCA GGTGATGCAG 
GGTCATCACG AAGTAGAGGG AGCATGGCAG CATCGTAAGC TCCGCCTCCA CCAGCGCCTG 
CAGTTATGTG TGTTTCAACA GGATGTTAAA CAGGTTTTGG ACTGGGTGGA GCAGCATGGA 
GAGGTTTTCC TGAACAAACA CACGGGTGTG GGGAAGTCCT TGCACCGGGC ACGGGCGCTG 
CAGAAGCGAC ACGATGACTT TCAGCAGGTT GCACAGAATA CGTACACCAA TGCAGAGAAG 
CTTCTGGAGG CAGCAGACCA GTTGGCGCAG TCTGGAGAGT GTGAGGAAGA GGAGATCTAC 
CAGGCAGCCA GAGACCTGGA GCTGCGAATG CAGGCCTTCA TTCAGCGCGT TGAACAGAGG 
AAGCTGCTGC TGGACCTCGC CGTATCCTTC TACACGCACA CAAAGGAGCT CTCTGCATGG 
ATGGAGGGCC TCCAGAAGCA GCTCTCCTCA GACCTCCTCG TGTGCCCGGA CACTGTGGAA 
GCTCTGCAGG CTTTAATCAG CCAACAGCAG CAGCAGCAGA CCAACACTCA GGAAGCTGCG 
ATGAGTGTCA TCCGGGAAGG AGAAGACCTC ATCCTGCAAC TCAGGCCTCA GGGCAGCTCG 
GTGGCCCATG TGGAATCGGT TTTGCAGCAA CTGGAAGAGT CTCATGTTCA GCTTGAGGAA 
CTTTTTCACC AGAGGAAGAT CCGAGTGGAC ATTTTCCTGC AGTTCCGCAT ACTGCACCAG 
TGCACGCTGG AGGTTACAGC TGAGATGGAT GCTTGGAAAA TGGACTTGGA AAAACAATCC 
CAGGAATTTT CCTCGGAGAA GGTCGCCTCA AATCGGCTTG CTGAAACCAA CCAGGACAAG 
CTGACCCAGG ATAAGCTGGC ACTAGGACAG TCCCGGCTTG TGGAGTCCAG CCCGGAAGCG 
TTAACACTAG CACAGCAGCG GATTCACAGG CACATGGAAC GGAGGCTGGC GATGAACAAC 
ATGATATATG AGGTCATCCA GCAAGGTCAT GACCTTCAGC AGTACATCTC GGAGGTGCAG 
GCATCAGGTA TCGAGCTGTC AGACGTGGAG GGTGGGCTGT CCTCTCAGGT GGAGGAGCTG 
CAGCGCCTCC TGCAGGACAA ACAGAAGGAG CTGCAGCAGA TTGCCGATCA CACGCATACG 
CTGCTGGAGC AGAACCTGCA GCTGAGACAC CTCCAGAGCC AGGTCAAGCA GGTGCTCGGC 
TGGATCTCAG AGGGGGAGGT GATGCTGTCC TCCTGCATGT TGAATTCCAG CTGTCTGTCA 
GAGGCGGAGC AGCTGCAGAA GGAACACGAG CGCCTCCAAC AGGCCATAGA GGCTCTGCTG 
CACGCAGACT CCCTGCAGAG GACTCATCAG GTGGCGCTGG CCCTGCAGCA GAGAGCGGAG 
CTGCTGGTCA GGTCTGGCCA CTATGATCCA GATGCAGTGA GGTCCTGCGC TCAGACTGTA 
GCGCTGCATT GGCAGACACT AATGCTCAGG ATTGAAGACA GACTCAAACT GGTCAATGCG 
TCGGCTGCTT TCTACAGGAC ATCCCAGCAG GTATGTGGGG TCCTGGAGAG TCTGGAGCAG 
GAATACCGCA GGGAGGAGGA CTGGTGTGCA GGAGGAGAGA GGCAACCAGA GCAACTGCTG 
CCGCTAATCA ATAAACACAT GGAGCAGAAG GAGGCTTTTC TGAAGGCCTG TACTCTTGCG 
AGACGGAATG CAGAAGTTTT TCTCAAGTAT ATCCATCGCA ATAGCGTCAC CATGGCAAAT 
ATCTCTGGCC ATAGCATCAC TGTCTCTGGG GTCTCTGAGG TTACTGGCCA TTCCAGAGTC 
CCTGAACAAC AGGTCAAAGG TATCCTTAGT GAGCTGCTCC AGAGGGAAAA CAGAGTTCTT 
CATTTCTGGA CTTTAAAGAA ACGCAGATTG GATCAGTGCC AGCAGTATCT GATGTTCCAA 
ACCCACGCAC AGCAGGCTCT TGATTGGTTG CAGCAGTCAG GTGACCATTA CTTGGCCACA 
CATACGTCAC CAGGTGCAAC CATGGCTGAG ACACAGGAGC TGCTGAACCA ATACAGAGAG 
TTCTGCATTT CTGCAAAGCA CACACAGGAG AAGGTTCACC TGCTGATCCA GCTGGCTGAG 
AGTATGCTGG CTAAAGGTCA CACCCACCGC ACTGAGCTCC GCCGCTGCGT TTCAACAGTG 
GATAAGCGCT ACCGTGACTT CTCTGTCAGG ATGGGACAAT ATCGCCACCT GCTAGAGACA 
GCGCTGGGAG GCTGTTCTCA GGACAATAAA GACCTGGAGC TGGAGCTGAT CCCCAATAGT 
CTCTCGGACT CTGACCCAGA GGTCAACCTA TCTGACCCAA GTCACCAAGT CAGTGATGAG 
AAAAGACGGT CCGCCCGCAA AAAGGAGTAC ATCATGGCAG AGCTCCTTCA GACAGAACGA 
GTCTATGTCA GAGACCTGCA GGAGTGCATA GAGACGTACC TGTGGGAGAT GACAAGCGGT 
TCAGAAGATG TTCCACTCGG TCTTGCCAAC AGTCACTACA CTGTGTTTGG AAACATTCAG 
GACATCTACG AGTTCCATAA CAGTATTTTC TTAAAGGAGT TAGAGAACTA CGAGCAGCTT 
CCTGAAGACG TGGGACACTG TTTCGTCACC TGGGCTGATA AGTTCCACAT GTATGTGACG 
TACTGCAGAA ACAAACCAGA CTCCAGTTTA TTGATCCAAC AGCATGGAGT GGGATTTTTT 
GAGGAGGTGC AGAGGAGACA CGGACTTGCT AACTCCATCT CCTCTGCGCT GATTAAACCG 
GTCCAGAGGA TCACCAAGTA CCAGCTGTTG CTTAAGGAGC TGCTGGCTTG CTGCGAGGAA 
GGCAAGGGGG AGATCAAGGA AGGCCTGGAC GTGATGCTGA GTGTCCCAAA GAGAGCCAAC 
GATGCAATGC ACGTCAGCAT GTTAGAAGGT CTGGAGGACG GGGTGGAGGT GCAGGGAGAG 
CTCCTCCTGC AGGACTCTTT CCTGGTCTGG GAGCCTAAGT CTCTGATAAG AAAGGGGCGG 
GACAGACACT TATTCTTGTT TGAACTGTCA CTCATCTTTA GCAAAGAGAT TAAAGACTCG 
TCCGGCCGCA CCAAGTATCT CTACAAAAGT CGCTTGAGGA CGTCAGAGCT GGGAGTGACG 
GAGCACATAG AGGGGGATCA GTGTAAGTTT GCACTGTGGG TGGGACGAAC GCCGACATCA 
GACAACAAGA CCGTCCTGAA GGCTTCATCC CTCGAGCTAA AGCAGGAGTG GGTTCGCAGT 
ATCCGACAGG TGATCCAGGA GAGGCGGGGT CACCTGCGAG GCGCACTGAG GGAGCCTATC 
CCGCTCCCAA AAACCCCCAA CCCCACGCTG GGACGACAGC GCAGCATCAC CCGCAGGGAG 
ACGAGCGAGG ACGCAGACAG CCTGGGTGAT GCCAGCAGTC AGCCTGACAC GGTTTCCATC 
GCATCACGAA CGTCGCAGAA CACTGCCGAC AGCGACAAGC TCTCTGGGGG CTGCGAGCTA 
GTGGTGGTGC TGCTGGACTT CACCTCTGGA GGACCGGGAG AGCTCAGCGT CCGCTGCGGC 
CAGACGGTGG AGCTGGTGGA GCGCTCTGCA GACCGGCCTG GCTGGTGTCT GATCAGAACC 
ACGGATCAGA CGCCCCAGCA AGAGGGTTTG CTGCCCATGA GCGCCCTCTG CCTGTCGCAC 
TCCCACAGTG CAGGTGACAT GGAGGGGCTC CTCCCAGCCT CCACCACCTC CTCTAGCACC 
TCCTCCACCT GCACCACCAC CTCCTCCTCT CTCAACTCCT CCTCCACCAC CACCTCCTCA 
TCCACCATGA GCGCAGCAAA GGATTCCAGT CTGTTCAGTT CTGAGGGTTC AGGCCTCCCG 
ACTCAGACCA GCAGTCAAAG TGCCACCTCC CCCACCGTAG GTGGACCGGG CGGAGCTCCA 
GTAGGTGCAG TAGGGTCTCC GGGGCCCAAA CGGAGCGGCT CTGGCACAGG CAACACGTTA 
AAGCGGTGGT TGACCAGCCC TGTGCGAAGG CTGAGTCAGG GAAAAGCTGA CGGACAGAAA 
AAACCTCCAA TCAGAGCCAG GAGGCGGGAC AACAAGCTCG AGCTGGCCAC GCCCCTTACC 
GTAAATACAC AGATGCAGAA GGCAAGAAAG GAGGAGGCGG GGCAAAGTGG AGGGGAGGAG 
GAGCAAGAGG AAGAAAGCCA CACCCCATTA CCTCCACCGA TGCAGATTAT GAAAGACCCC 
AACAATCCAG AGGCTCTGGA CTCAGATCAG GGATCAAACG AGTCCGATAC TGAACAGAGA 
AACAAAGCAC TAAGAGGACG AATGTTTGTT GTTAATGAGA TGGTCCAATC AGAGAAGGAC 
TACGTAAAGG ACCTAGGAGT GATTGTTGAG GGCTTCATGT CCAGGCTTGA GGTCAGGGGG 
ATCCCAGAAG ACATGAGGGG AAAAGACAAG ATCGTGTTTG GCAACATCCA GCAGATCTAC 
GACTGGCACC GCGACTTCTT TCTGGTGGAG CTAGAGCGCT GCATCCAGAA CCACGATCTG 
CTGGCCGACC TGTTCATCAG ACATGAGCGT CGTCTGCACA TGTATGTGGT TTACTGTCAA 
AACAAGCCCA AGTCGGAGTT CATCGTCATT GAGTATGAGA CCTTCTTTGA GGAGATCCAG 
CATGAGATCA GCTGCAGGAT GTCGATCAGT GATTATCTGA TCAAACCCAT CCAGAGAATC 
ACCAAGTACC AGCTACTACT GAAGGACTTC TTCAAATATA CGACCAAAGC AGGGCTGGAC 
ACTGAGGAGA TTGAGAAAGC GGTGGAGCTG ATGTCATTGG TTCCAAAGCG CTGCAACGAC 
ATGATGAACC TGGGACGCCT GCAGGGATAT GAGGGGAAGT TGACGTCTCA GGGGAAGCTG 
CTGCAGCAGG AGACCTTCTG CGTTTGGGAG CAGGACGGGG GAGTCTTGTC TCGCAGCAAA 
GAGCGCAGAG TCTTCCTCTT CGAGCAGATC ATCATCTTCA GTGAACTGCT CCGGAAAGGC 
TCCAACAATC CAGGATACCA GTTCAAGAAC AGCATCAAGG TGAGTTACCT GGCCATGCAG 
GACAGCGTAG ACGGCGATCC CTGTAAGTTT GTGCTGTGGT CCCGGGGCTC GGCAGAGCGC 
TTCACTCTAC AAGCGTCCTC CGCCAGCATC AAGATGACCT GGGTGGAGAC CATTGCCACT 
CTGCTGGACG CCCAGAACAA CTTCCTCTCT GCTCTCCAGT CACCTATTGA GTACCAGAGA 
AAAGAGGGCG GGATTTCAGT GACCCGTCCA CTGTCGTCTG GTCGTCCACC GTCTGCTCCG 
CCCACGCCTA ACGGCCATGC CACTCAACCT CCTGCTGACA GTGAGGATGA CCAGGAGCTG 
GTTCAGGTGC TGCAGGACTT TGTGGCCGTG CGGGAGGACG AGATTTCCGT GTTTCGGGGA 
GAGAAAGTCC AGATTCTGTC GTCCAACCAG CAGGGTCAGA GTCTTGTTTA CAGACCTGCC 
AACACCGACT CGCCCGCCGC CGAGGGCTGG GTGCCACGAA GCGTGCTCAA AACACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011481279.2
CDS	469..7548
Translation	MNPADGGGEAGPDGQATAGSGKGESVRAVDVLTVLREKVAFVSGGRDKRGGPILTFPARTNHDRIKQEDLSRLVTYLSTIPSEDVRARGFTVVVDMRGSKWDSVKPVLRTLQESFSSNIHTALLIKPDTFWQKHKTNLGSAKLSFETSLVSVEGLSRLIDPSQLTADLGGSLEYDHVEWTELRLGLEEFSGAATQLLTQLEQLEAGLSRIPEPQDIDEARKLLEEHTRLRNSIATAPVDALDREGKCLLQRMRLGSSHGEASNESGGGGGTWLSGGADFQSVAPKVSSLLDRLQGARSHLLQCWGDKKLHLDQALQLHLFEQDATKMSEWIAHSKELFMQSLAEVGQNHQHALDLQTQHQHFTANCMNGSVNVDSVVTVAARLAEAGHYGAERIALVSSKLQDNWKSMTSALEERSNMLAMATGFHQGAEQFLARVEGWVKLCSEGPLPSTTAELEPAIKKHQELNEEISANYTQVSESGKALLEVLQRCPTTDSDESATKPDFTAATHTIMGVLHQVMQGHHEVEGAWQHRKLRLHQRLQLCVFQQDVKQVLDWVEQHGEVFLNKHTGVGKSLHRARALQKRHDDFQQVAQNTYTNAEKLLEAADQLAQSGECEEEEIYQAARDLELRMQAFIQRVEQRKLLLDLAVSFYTHTKELSAWMEGLQKQLSSDLLVCPDTVEALQALISQQQQQQTNTQEAAMSVIREGEDLILQLRPQGSSVAHVESVLQQLEESHVQLEELFHQRKIRVDIFLQFRILHQCTLEVTAEMDAWKMDLEKQSQEFSSEKVASNRLAETNQDKLTQDKLALGQSRLVESSPEALTLAQQRIHRHMERRLAMNNMIYEVIQQGHDLQQYISEVQASGIELSDVEGGLSSQVEELQRLLQDKQKELQQIADHTHTLLEQNLQLRHLQSQVKQVLGWISEGEVMLSSCMLNSSCLSEAEQLQKEHERLQQAIEALLHADSLQRTHQVALALQQRAELLVRSGHYDPDAVRSCAQTVALHWQTLMLRIEDRLKLVNASAAFYRTSQQVCGVLESLEQEYRREEDWCAGGERQPEQLLPLINKHMEQKEAFLKACTLARRNAEVFLKYIHRNSVTMANISGHSITVSGVSEVTGHSRVPEQQVKGILSELLQRENRVLHFWTLKKRRLDQCQQYLMFQTHAQQALDWLQQSGDHYLATHTSPGATMAETQELLNQYREFCISAKHTQEKVHLLIQLAESMLAKGHTHRTELRRCVSTVDKRYRDFSVRMGQYRHLLETALGGCSQDNKDLELELIPNSLSDSDPEVNLSDPSHQVSDEKRRSARKKEYIMAELLQTERVYVRDLQECIETYLWEMTSGSEDVPLGLANSHYTVFGNIQDIYEFHNSIFLKELENYEQLPEDVGHCFVTWADKFHMYVTYCRNKPDSSLLIQQHGVGFFEEVQRRHGLANSISSALIKPVQRITKYQLLLKELLACCEEGKGEIKEGLDVMLSVPKRANDAMHVSMLEGLEDGVEVQGELLLQDSFLVWEPKSLIRKGRDRHLFLFELSLIFSKEIKDSSGRTKYLYKSRLRTSELGVTEHIEGDQCKFALWVGRTPTSDNKTVLKASSLELKQEWVRSIRQVIQERRGHLRGALREPIPLPKTPNPTLGRQRSITRRETSEDADSLGDASSQPDTVSIASRTSQNTADSDKLSGGCELVVVLLDFTSGGPGELSVRCGQTVELVERSADRPGWCLIRTTDQTPQQEGLLPMSALCLSHSHSAGDMEGLLPASTTSSSTSSTCTTTSSSLNSSSTTTSSSTMSAAKDSSLFSSEGSGLPTQTSSQSATSPTVGGPGGAPVGAVGSPGPKRSGSGTGNTLKRWLTSPVRRLSQGKADGQKKPPIRARRRDNKLELATPLTVNTQMQKARKEEAGQSGGEEEQEEESHTPLPPPMQIMKDPNNPEALDSDQGSNESDTEQRNKALRGRMFVVNEMVQSEKDYVKDLGVIVEGFMSRLEVRGIPEDMRGKDKIVFGNIQQIYDWHRDFFLVELERCIQNHDLLADLFIRHERRLHMYVVYCQNKPKSEFIVIEYETFFEEIQHEISCRMSISDYLIKPIQRITKYQLLLKDFFKYTTKAGLDTEEIEKAVELMSLVPKRCNDMMNLGRLQGYEGKLTSQGKLLQQETFCVWEQDGGVLSRSKERRVFLFEQIIIFSELLRKGSNNPGYQFKNSIKVSYLAMQDSVDGDPCKFVLWSRGSAERFTLQASSASIKMTWVETIATLLDAQNNFLSALQSPIEYQRKEGGISVTRPLSSGRPPSAPPTPNGHATQPPADSEDDQELVQVLQDFVAVREDEISVFRGEKVQILSSNQQGQSLVYRPANTDSPAAEGWVPRSVLKTH

Target ORF information:

RefSeq Version	XM_011482977.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes kalirin (LOC101166941), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011482977.3

ORF Insert Sequence:

ATGAATCCGG CAGATGGAGG AGGAGAAGCG GGCCCGGACG GCCAGGCGAC GGCAGGTTCT 
GGTAAAGGGG AGTCTGTCCG GGCGGTCGAT GTCTTGACGG TGCTCAGAGA AAAAGTGGCC 
TTTGTGTCGG GTGGACGCGA CAAACGAGGT GGACCAATTC TCACCTTTCC TGCTAGGACC 
AATCACGATC GAATAAAACA GGAAGACCTC AGCCGCTTGG TTACTTATTT ATCAACTATC 
CCTAGCGAGG ATGTGCGTGC CCGTGGCTTT ACCGTTGTGG TGGATATGCG GGGCAGTAAG 
TGGGACAGCG TGAAGCCGGT TCTGCGAACA CTACAGGAGT CCTTTTCCTC AAACATCCAC 
ACAGCACTAC TCATCAAACC GGACACCTTC TGGCAGAAAC ACAAGACCAA CCTAGGCAGT 
GCCAAGCTGA GTTTCGAGAC CAGTCTGGTG TCAGTGGAGG GTTTGTCCCG ACTTATTGAT 
CCCTCTCAGC TGACCGCTGA CCTCGGAGGT TCATTGGAGT ATGACCATGT GGAGTGGACT 
GAGCTGCGTC TTGGTTTAGA GGAGTTCTCA GGGGCTGCCA CACAGCTACT AACCCAACTG 
GAGCAGCTGG AAGCCGGGCT GAGCCGCATA CCCGAGCCTC AGGACATTGA TGAGGCCCGC 
AAACTCTTGG AAGAACACAC TCGCCTCCGC AATAGCATCG CCACTGCGCC TGTTGATGCC 
CTCGACCGAG AAGGGAAGTG TTTACTTCAG CGGATGCGCC TAGGTTCATC CCACGGTGAA 
GCCTCAAATG AGAGTGGGGG TGGTGGGGGA ACTTGGTTGT CAGGAGGAGC AGATTTCCAG 
AGCGTGGCGC CGAAGGTTTC GTCTTTATTG GACCGGCTGC AGGGGGCTCG CAGTCACTTA 
CTGCAGTGCT GGGGTGATAA GAAGCTGCAC TTGGACCAGG CACTGCAACT CCACCTGTTT 
GAACAGGATG CTACCAAGAT GTCAGAGTGG ATCGCTCACA GTAAAGAGCT CTTCATGCAG 
AGTCTGGCAG AAGTCGGTCA GAACCACCAG CACGCTCTCG ATCTACAAAC GCAGCACCAG 
CATTTCACCG CCAACTGCAT GAACGGCAGT GTGAATGTGG ACAGTGTCGT TACCGTGGCA 
GCAAGGCTCG CGGAGGCCGG CCACTACGGA GCAGAGCGGA TTGCTTTGGT GTCGTCAAAG 
TTACAGGACA ACTGGAAGTC AATGACAAGT GCTCTGGAAG AGCGGAGCAA CATGCTTGCC 
ATGGCAACCG GTTTCCATCA AGGGGCAGAG CAGTTTCTAG CCCGAGTGGA GGGATGGGTT 
AAACTGTGCT CTGAAGGGCC ATTGCCATCA ACAACAGCCG AGCTGGAACC TGCAATAAAA 
AAACACCAGG AGCTCAATGA GGAGATTTCA GCCAACTACA CACAGGTCAG TGAAAGCGGT 
AAGGCATTGC TGGAGGTCCT TCAGCGTTGC CCCACAACAG ATTCTGACGA ATCAGCAACC 
AAACCAGATT TCACTGCGGC CACACACACC ATTATGGGGG TTCTACACCA GGTGATGCAG 
GGTCATCACG AAGTAGAGGG AGCATGGCAG CATCGTAAGC TCCGCCTCCA CCAGCGCCTG 
CAGTTATGTG TGTTTCAACA GGATGTTAAA CAGGTTTTGG ACTGGGTGGA GCAGCATGGA 
GAGGTTTTCC TGAACAAACA CACGGGTGTG GGGAAGTCCT TGCACCGGGC ACGGGCGCTG 
CAGAAGCGAC ACGATGACTT TCAGCAGGTT GCACAGAATA CGTACACCAA TGCAGAGAAG 
CTTCTGGAGG CAGCAGACCA GTTGGCGCAG TCTGGAGAGT GTGAGGAAGA GGAGATCTAC 
CAGGCAGCCA GAGACCTGGA GCTGCGAATG CAGGCCTTCA TTCAGCGCGT TGAACAGAGG 
AAGCTGCTGC TGGACCTCGC CGTATCCTTC TACACGCACA CAAAGGAGCT CTCTGCATGG 
ATGGAGGGCC TCCAGAAGCA GCTCTCCTCA GACCTCCTCG TGTGCCCGGA CACTGTGGAA 
GCTCTGCAGG CTTTAATCAG CCAACAGCAG CAGCAGCAGA CCAACACTCA GGAAGCTGCG 
ATGAGTGTCA TCCGGGAAGG AGAAGACCTC ATCCTGCAAC TCAGGCCTCA GGGCAGCTCG 
GTGGCCCATG TGGAATCGGT TTTGCAGCAA CTGGAAGAGT CTCATGTTCA GCTTGAGGAA 
CTTTTTCACC AGAGGAAGAT CCGAGTGGAC ATTTTCCTGC AGTTCCGCAT ACTGCACCAG 
TGCACGCTGG AGGTTACAGC TGAGATGGAT GCTTGGAAAA TGGACTTGGA AAAACAATCC 
CAGGAATTTT CCTCGGAGAA GGTCGCCTCA AATCGGCTTG CTGAAACCAA CCAGGACAAG 
CTGACCCAGG ATAAGCTGGC ACTAGGACAG TCCCGGCTTG TGGAGTCCAG CCCGGAAGCG 
TTAACACTAG CACAGCAGCG GATTCACAGG CACATGGAAC GGAGGCTGGC GATGAACAAC 
ATGATATATG AGGTCATCCA GCAAGGTCAT GACCTTCAGC AGTACATCTC GGAGGTGCAG 
GCATCAGGTA TCGAGCTGTC AGACGTGGAG GGTGGGCTGT CCTCTCAGGT GGAGGAGCTG 
CAGCGCCTCC TGCAGGACAA ACAGAAGGAG CTGCAGCAGA TTGCCGATCA CACGCATACG 
CTGCTGGAGC AGAACCTGCA GCTGAGACAC CTCCAGAGCC AGGTCAAGCA GGTGCTCGGC 
TGGATCTCAG AGGGGGAGGT GATGCTGTCC TCCTGCATGT TGAATTCCAG CTGTCTGTCA 
GAGGCGGAGC AGCTGCAGAA GGAACACGAG CGCCTCCAAC AGGCCATAGA GGCTCTGCTG 
CACGCAGACT CCCTGCAGAG GACTCATCAG GTGGCGCTGG CCCTGCAGCA GAGAGCGGAG 
CTGCTGGTCA GGTCTGGCCA CTATGATCCA GATGCAGTGA GGTCCTGCGC TCAGACTGTA 
GCGCTGCATT GGCAGACACT AATGCTCAGG ATTGAAGACA GACTCAAACT GGTCAATGCG 
TCGGCTGCTT TCTACAGGAC ATCCCAGCAG GTATGTGGGG TCCTGGAGAG TCTGGAGCAG 
GAATACCGCA GGGAGGAGGA CTGGTGTGCA GGAGGAGAGA GGCAACCAGA GCAACTGCTG 
CCGCTAATCA ATAAACACAT GGAGCAGAAG GAGGCTTTTC TGAAGGCCTG TACTCTTGCG 
AGACGGAATG CAGAAGTTTT TCTCAAGTAT ATCCATCGCA ATAGCGTCAC CATGGCAAAT 
ATCTCTGGCC ATAGCATCAC TGTCTCTGGG GTCTCTGAGG TTACTGGCCA TTCCAGAGTC 
CCTGAACAAC AGGTCAAAGG TATCCTTAGT GAGCTGCTCC AGAGGGAAAA CAGAGTTCTT 
CATTTCTGGA CTTTAAAGAA ACGCAGATTG GATCAGTGCC AGCAGTATCT GATGTTCCAA 
ACCCACGCAC AGCAGGCTCT TGATTGGTTG CAGCAGTCAG GTGACCATTA CTTGGCCACA 
CATACGTCAC CAGGTGCAAC CATGGCTGAG ACACAGGAGC TGCTGAACCA ATACAGAGAG 
TTCTGCATTT CTGCAAAGCA CACACAGGAG AAGGTTCACC TGCTGATCCA GCTGGCTGAG 
AGTATGCTGG CTAAAGGTCA CACCCACCGC ACTGAGCTCC GCCGCTGCGT TTCAACAGTG 
GATAAGCGCT ACCGTGACTT CTCTGTCAGG ATGGGACAAT ATCGCCACCT GCTAGAGACA 
GCGCTGGGAG GCTGTTCTCA GGACAATAAA GACCTGGAGC TGGAGCTGAT CCCCAATAGT 
CTCTCGGACT CTGACCCAGA GGTCAACCTA TCTGACCCAA GTCACCAAGT CAGTGATGAG 
AAAAGACGGT CCGCCCGCAA AAAGGAGTAC ATCATGGCAG AGCTCCTTCA GACAGAACGA 
GTCTATGTCA GAGACCTGCA GGAGTGCATA GAGACGTACC TGTGGGAGAT GACAAGCGGT 
TCAGAAGATG TTCCACTCGG TCTTGCCAAC AGTCACTACA CTGTGTTTGG AAACATTCAG 
GACATCTACG AGTTCCATAA CAGTATTTTC TTAAAGGAGT TAGAGAACTA CGAGCAGCTT 
CCTGAAGACG TGGGACACTG TTTCGTCACC TGGGCTGATA AGTTCCACAT GTATGTGACG 
TACTGCAGAA ACAAACCAGA CTCCAGTTTA TTGATCCAAC AGCATGGAGT GGGATTTTTT 
GAGGAGGTGC AGAGGAGACA CGGACTTGCT AACTCCATCT CCTCTGCGCT GATTAAACCG 
GTCCAGAGGA TCACCAAGTA CCAGCTGTTG CTTAAGGAGC TGCTGGCTTG CTGCGAGGAA 
GGCAAGGGGG AGATCAAGGA AGGCCTGGAC GTGATGCTGA GTGTCCCAAA GAGAGCCAAC 
GATGCAATGC ACGTCAGCAT GTTAGAAGGT CTGGAGGACG GGGTGGAGGT GCAGGGAGAG 
CTCCTCCTGC AGGACTCTTT CCTGGTCTGG GAGCCTAAGT CTCTGATAAG AAAGGGGCGG 
GACAGACACT TATTCTTGTT TGAACTGTCA CTCATCTTTA GCAAAGAGAT TAAAGACTCG 
TCCGGCCGCA CCAAGTATCT CTACAAAAGT CGCTTGAGGA CGTCAGAGCT GGGAGTGACG 
GAGCACATAG AGGGGGATCA GTGTAAGTTT GCACTGTGGG TGGGACGAAC GCCGACATCA 
GACAACAAGA CCGTCCTGAA GGCTTCATCC CTCGAGCTAA AGCAGGAGTG GGTTCGCAGT 
ATCCGACAGG TGATCCAGGA GAGGCGGGGT CACCTGCGAG GCGCACTGAG GGAGCCTATC 
CCGCTCCCAA AAACCCCCAA CCCCACGCTG GGACGACAGC GCAGCATCAC CCGCAGGGAG 
ACGAGCGAGG ACGCAGACAG CCTGGGTGAT GCCAGCAGTC AGCCTGACAC GGTTTCCATC 
GCATCACGAA CGTCGCAGAA CACTGCCGAC AGCGACAAGC TCTCTGGGGG CTGCGAGCTA 
GTGGTGGTGC TGCTGGACTT CACCTCTGGA GGACCGGGAG AGCTCAGCGT CCGCTGCGGC 
CAGACGGTGG AGCTGGTGGA GCGCTCTGCA GACCGGCCTG GCTGGTGTCT GATCAGAACC 
ACGGATCAGA CGCCCCAGCA AGAGGGTTTG CTGCCCATGA GCGCCCTCTG CCTGTCGCAC 
TCCCACAGTG CAGGTGACAT GGAGGGGCTC CTCCCAGCCT CCACCACCTC CTCTAGCACC 
TCCTCCACCT GCACCACCAC CTCCTCCTCT CTCAACTCCT CCTCCACCAC CACCTCCTCA 
TCCACCATGA GCGCAGCAAA GGATTCCAGT CTGTTCAGTT CTGAGGGTTC AGGCCTCCCG 
ACTCAGACCA GCAGTCAAAG TGCCACCTCC CCCACCGTAG GTGGACCGGG CGGAGCTCCA 
GTAGGTGCAG TAGGGTCTCC GGGGCCCAAA CGGAGCGGCT CTGGCACAGG CAACACGTTA 
AAGCGGTGGT TGACCAGCCC TGTGCGAAGG CTGAGTCAGG GAAAAGCTGA CGGACAGAAA 
AAACCTCCAA TCAGAGCCAG GAGGCGGGAC AACAAGCTCG AGCTGGCCAC GCCCCTTACC 
GTAAATACAC AGATGCAGAA GGCAAGAAAG GAGGAGGCGG GGCAAAGTGG AGGGGAGGAG 
GAGCAAGAGG AAGAAAGCCA CACCCCATTA CCTCCACCGA TGCAGATTAT GAAAGACCCC 
AACAATCCAG AGGCTCTGGA CTCAGATCAG GGATCAAACG AGTCCGATAC TGAACAGAGA 
AACAAAGCAC TAAGAGGACG AATGTTTGTT GTTAATGAGA TGGTCCAATC AGAGAAGGAC 
TACGTAAAGG ACCTAGGAGT GATTGTTGAG GGCTTCATGT CCAGGCTTGA GGTCAGGGGG 
ATCCCAGAAG ACATGAGGGG AAAAGACAAG ATCGTGTTTG GCAACATCCA GCAGATCTAC 
GACTGGCACC GCGACTTCTT TCTGGTGGAG CTAGAGCGCT GCATCCAGAA CCACGATCTG 
CTGGCCGACC TGTTCATCAG ACATGAGCGT CGTCTGCACA TGTATGTGGT TTACTGTCAA 
AACAAGCCCA AGTCGGAGTT CATCGTCATT GAGTATGAGA CCTTCTTTGA GGAGATCCAG 
CATGAGATCA GCTGCAGGAT GTCGATCAGT GATTATCTGA TCAAACCCAT CCAGAGAATC 
ACCAAGTACC AGCTACTACT GAAGGACTTC TTCAAATATA CGACCAAAGC AGGGCTGGAC 
ACTGAGGAGA TTGAGAAAGC GGTGGAGCTG ATGTCATTGG TTCCAAAGCG CTGCAACGAC 
ATGATGAACC TGGGACGCCT GCAGGGATAT GAGGGGAAGT TGACGTCTCA GGGGAAGCTG 
CTGCAGCAGG AGACCTTCTG CGTTTGGGAG CAGGACGGGG GAGTCTTGTC TCGCAGCAAA 
GAGCGCAGAG TCTTCCTCTT CGAGCAGATC ATCATCTTCA GTGAACTGCT CCGGAAAGGC 
TCCAACAATC CAGGATACCA GTTCAAGAAC AGCATCAAGG TGAGTTACCT GGCCATGCAG 
GACAGCGTAG ACGGCGATCC CTGTAAGTTT GTGCTGTGGT CCCGGGGCTC GGCAGAGCGC 
TTCACTCTAC AAGCGTCCTC CGCCAGCATC AAGATGACCT GGGTGGAGAC CATTGCCACT 
CTGCTGGACG CCCAGAACAA CTTCCTCTCT GCTCTCCAGT CACCTATTGA GTACCAGAGA 
AAAGAGGGCG GGATTTCAGT GACCCGTCCA CTGTCGTCTG GTCGTCCACC GTCTGCTCCG 
CCCACGCCTA ACGGCCATGC CACTCAACCT CCTGCTGACA GTGAGGATGA CCAGGAGCTG 
GTTCAGGTGC TGCAGGACTT TGTGGCCGTG CGGGAGGACG AGATTTCCGT GTTTCGGGGA 
GAGAAAGTCC AGATTCTGTC GTCCAACCAG CAGGGTCAGA GTCTTGTTTA CAGACCTGCC 
AACACCGACT CGCCCGCCGC CGAGGGCTGG GTGCCACGAA GCGTGCTCAA AACACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101166941 ( XM_020707977.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020563636.1 Oryzias latipes kalirin-like (LOC101166941), transcript variant X3, mRNA.

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CloneID	OJa74580
Clone ID Related Accession (Same CDS sequence)	XM_020707977.1
Accession Version	XM_020707977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	kalirin-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019860.1) and transcript sequence (GFIO01025530.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 506 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAATCCGG CAGATGGAGG AGGAGAAGCG GGCCCGGACG GCCAGGCGAC GGCAGGTTCT 
GGTAAAGGGG AGTCTGTCCG GGCGGTCGAT GTCTTGACGG TGCTCAGAGA AAAAGTGGCC 
TTTGTGTCGG GTGGACGCGA CAAACGAGGT GGACCAATTC TCACCTTTCC TGCTAGGACC 
AATCACGATC GAATAAAACA GGAAGACCTC AGCCGCTTGG TTACTTATTT ATCAACTATC 
CCTAGCGAGG ATGTGCGTGC CCGTGGCTTT ACCGTTGTGG TGGATATGCG GGGCAGTAAG 
TGGGACAGCG TGAAGCCGGT TCTGCGAACA CTACAGGAGT CCTTTTCCTC AAACATCCAC 
ACAGCACTAC TCATCAAACC GGACACCTTC TGGCAGAAAC ACAAGACCAA CCTAGGCAGT 
GCCAAGCTGA GTTTCGAGAC CAGTCTGGTG TCAGTGGAGG GTTTGTCCCG ACTTATTGAT 
CCCTCTCAGC TGACCGCTGA CCTCGGAGGT TCATTGGAGT ATGACCATGT GGAGTGGACT 
GAGCTGCGTC TTGGTTTAGA GGAGTTCTCA GGGGCTGCCA CACAGCTACT AACCCAACTG 
GAGCAGCTGG AAGCCGGGCT GAGCCGCATA CCCGAGCCTC AGGACATTGA TGAGGCCCGC 
AAACTCTTGG AAGAACACAC TCGCCTCCGC AATAGCATCG CCACTGCGCC TGTTGATGCC 
CTCGACCGAG AAGGGAAGTG TTTACTTCAG CGGATGCGCC TAGGTTCATC CCACGGTGAA 
GCCTCAAATG AGAGTGGGGG TGGTGGGGGA ACTTGGTTGT CAGGAGGAGC AGATTTCCAG 
AGCGTGGCGC CGAAGGTTTC GTCTTTATTG GACCGGCTGC AGGGGGCTCG CAGTCACTTA 
CTGCAGTGCT GGGGTGATAA GAAGCTGCAC TTGGACCAGG CACTGCAACT CCACCTGTTT 
GAACAGGATG CTACCAAGAT GTCAGAGTGG ATCGCTCACA GTAAAGAGCT CTTCATGCAG 
AGTCTGGCAG AAGTCGGTCA GAACCACCAG CACGCTCTCG ATCTACAAAC GCAGCACCAG 
CATTTCACCG CCAACTGCAT GAACGGCAGT GTGAATGTGG ACAGTGTCGT TACCGTGGCA 
GCAAGGCTCG CGGAGGCCGG CCACTACGGA GCAGAGCGGA TTGCTTTGGT GTCGTCAAAG 
TTACAGGACA ACTGGAAGTC AATGACAAGT GCTCTGGAAG AGCGGAGCAA CATGCTTGCC 
ATGGCAACCG GTTTCCATCA AGGGGCAGAG CAGTTTCTAG CCCGAGTGGA GGGATGGGTT 
AAACTGTGCT CTGAAGGGCC ATTGCCATCA ACAACAGCCG AGCTGGAACC TGCAATAAAA 
AAACACCAGG AGCTCAATGA GGAGATTTCA GCCAACTACA CACAGGTCAG TGAAAGCGGT 
AAGGCATTGC TGGAGGTCCT TCAGCGTTGC CCCACAACAG ATTCTGACGA ATCAGCAACC 
AAACCAGATT TCACTGCGGC CACACACACC ATTATGGGGG TTCTACACCA GGTGATGCAG 
GGTCATCACG AAGTAGAGGG AGCATGGCAG CATCGTAAGC TCCGCCTCCA CCAGCGCCTG 
CAGTTATGTG TGTTTCAACA GGATGTTAAA CAGGTTTTGG ACTGGGTGGA GCAGCATGGA 
GAGGTTTTCC TGAACAAACA CACGGGTGTG GGGAAGTCCT TGCACCGGGC ACGGGCGCTG 
CAGAAGCGAC ACGATGACTT TCAGCAGGTT GCACAGAATA CGTACACCAA TGCAGAGAAG 
CTTCTGGAGG CAGCAGACCA GTTGGCGCAG TCTGGAGAGT GTGAGGAAGA GGAGATCTAC 
CAGGCAGCCA GAGACCTGGA GCTGCGAATG CAGGCCTTCA TTCAGCGCGT TGAACAGAGG 
AAGCTGCTGC TGGACCTCGC CGTATCCTTC TACACGCACA CAAAGGAGCT CTCTGCATGG 
ATGGAGGGCC TCCAGAAGCA GCTCTCCTCA GACCTCCTCG TGTGCCCGGA CACTGTGGAA 
GCTCTGCAGG CTTTAATCAG CCAACAGCAG CAGCAGCAGA CCAACACTCA GGAAGCTGCG 
ATGAGTGTCA TCCGGGAAGG AGAAGACCTC ATCCTGCAAC TCAGGCCTCA GGGCAGCTCG 
GTGGCCCATG TGGAATCGGT TTTGCAGCAA CTGGAAGAGT CTCATGTTCA GCTTGAGGAA 
CTTTTTCACC AGAGGAAGAT CCGAGTGGAC ATTTTCCTGC AGTTCCGCAT ACTGCACCAG 
TGCACGCTGG AGGTTACAGC TGAGATGGAT GCTTGGAAAA TGGACTTGGA AAAACAATCC 
CAGGAATTTT CCTCGGAGAA GGTCGCCTCA AATCGGCTTG CTGAAACCAA CCAGGACAAG 
CTGACCCAGG ATAAGCTGGC ACTAGGACAG TCCCGGCTTG TGGAGTCCAG CCCGGAAGCG 
TTAACACTAG CACAGCAGCG GATTCACAGG CACATGGAAC GGAGGCTGGC GATGAACAAC 
ATGATATATG AGGTCATCCA GCAAGGTCAT GACCTTCAGC AGTACATCTC GGAGGTGCAG 
GCATCAGGTA TCGAGCTGTC AGACGTGGAG GGTGGGCTGT CCTCTCAGGT GGAGGAGCTG 
CAGCGCCTCC TGCAGGACAA ACAGAAGGAG CTGCAGCAGA TTGCCGATCA CACGCATACG 
CTGCTGGAGC AGAACCTGCA GCTGAGACAC CTCCAGAGCC AGGTCAAGCA GGTGCTCGGC 
TGGATCTCAG AGGGGGAGGT GATGCTGTCC TCCTGCATGT TGAATTCCAG CTGTCTGTCA 
GAGGCGGAGC AGCTGCAGAA GGAACACGAG CGCCTCCAAC AGGCCATAGA GGCTCTGCTG 
CACGCAGACT CCCTGCAGAG GACTCATCAG GTGGCGCTGG CCCTGCAGCA GAGAGCGGAG 
CTGCTGGTCA GGTCTGGCCA CTATGATCCA GATGCAGTGA GGTCCTGCGC TCAGACTGTA 
GCGCTGCATT GGCAGACACT AATGCTCAGG ATTGAAGACA GACTCAAACT GGTCAATGCG 
TCGGCTGCTT TCTACAGGAC ATCCCAGCAG GTATGTGGGG TCCTGGAGAG TCTGGAGCAG 
GAATACCGCA GGGAGGAGGA CTGGTGTGCA GGAGGAGAGA GGCAACCAGA GCAACTGCTG 
CCGCTAATCA ATAAACACAT GGAGCAGAAG GAGGCTTTTC TGAAGGCCTG TACTCTTGCG 
AGACGGAATG CAGAAGTTTT TCTCAAGTAT ATCCATCGCA ATAGCGTCAC CATGGCAAAT 
ATCTCTGGCC ATAGCATCAC TGTCTCTGGG GTCTCTGAGG TTACTGGCCA TTCCAGAGTC 
CCTGAACAAC AGGTCAAAGG TATCCTTAGT GAGCTGCTCC AGAGGGAAAA CAGAGTTCTT 
CATTTCTGGA CTTTAAAGAA ACGCAGATTG GATCAGTGCC AGCAGTATCT GATGTTCCAA 
ACCCACGCAC AGCAGGCTCT TGATTGGTTG CAGCAGTCAG GTGACCATTA CTTGGCCACA 
CATACGTCAC CAGGTGCAAC CATGGCTGAG ACACAGGAGC TGCTGAACCA ATACAGAGAG 
TTCTGCATTT CTGCAAAGGT GCACACACAG GAGAAGGTTC ACCTGCTGAT CCAGCTGGCT 
GAGAGTATGC TGGCTAAAGG TCACACCCAC CGCACTGAGC TCCGCCGCTG CGTTTCAACA 
GTGGATAAGC GCTACCGTGA CTTCTCTGTC AGGATGGGAC AATATCGCCA CCTGCTAGAG 
ACAGCGCTGG GAGGCTGTTC TCAGGACAAT AAAGACCTGG AGCTGGAGCT GATCCCCAAT 
AGTCTCTCGG ACTCTGACCC AGAGGTCAAC CTATCTGACC CAAGTCACCA AGTCAGTGAT 
GAGAAAAGAC GGTCCGCCCG CAAAAAGGAG TACATCATGG CAGAGCTCCT TCAGACAGAA 
CGAGTCTATG TCAGAGACCT GCAGGAGTGC ATAGAGACGT ACCTGTGGGA GATGACAAGC 
GGTTCAGAAG ATGTTCCACT CGGTCTTGCC AACAGTCACT ACACTGTGTT TGGAAACATT 
CAGGACATCT ACGAGTTCCA TAACAGTATT TTCTTAAAGG AGTTAGAGAA CTACGAGCAG 
CTTCCTGAAG ACGTGGGACA CTGTTTCGTC ACCTGGGCTG ATAAGTTCCA CATGTATGTG 
ACGTACTGCA GAAACAAACC AGACTCCAGT TTATTGATCC AACAGCATGG AGTGGGATTT 
TTTGAGGAGG TGCAGAGGAG ACACGGACTT GCTAACTCCA TCTCCTCTGC GCTGATTAAA 
CCGGTCCAGA GGATCACCAA GTACCAGCTG TTGCTTAAGG AGCTGCTGGC TTGCTGCGAG 
GAAGGCAAGG GGGAGATCAA GGAAGGCCTG GACGTGATGC TGAGTGTCCC AAAGAGAGCC 
AACGATGCAA TGCACGTCAG CATGTTAGAA GGTCTGGAGG ACGGGGTGGA GGTGCAGGGA 
GAGCTCCTCC TGCAGGACTC TTTCCTGGTC TGGGAGCCTA AGTCTCTGAT AAGAAAGGGG 
CGGGACAGAC ACTTATTCTT GTTTGAACTG TCACTCATCT TTAGCAAAGA GATTAAAGAC 
TCGTCCGGCC GCACCAAGTA TCTCTACAAA AGTCGCTTGA GGACGTCAGA GCTGGGAGTG 
ACGGAGCACA TAGAGGGGGA TCAGTGTAAG TTTGCACTGT GGGTGGGACG AACGCCGACA 
TCAGACAACA AGACCGTCCT GAAGGCTTCA TCCCTCGAGC TAAAGCAGGA GTGGGTTCGC 
AGTATCCGAC AGGTGATCCA GGAGAGGCGG GGTCACCTGC GAGGCGCACT GAGGGAGCCT 
ATCCCGCTCC CAAAAACCCC CAACCCCACG CTGGGACGAC AGCGCAGCAT CACCCGCAGG 
GAGACGAGCG AGGACGCAGA CAGCCTGGGT GATGCCAGCA GTCAGCCTGA CACGGTTTCC 
ATCGCATCAC GAACGTCGCA GAACACTGCC GACAGCGACA AGCTCTCTGG GGGCTGCGAG 
CTAGTGGTGG TGCTGCTGGA CTTCACCTCT GGAGGACCGG GAGAGCTCAG CGTCCGCTGC 
GGCCAGACGG TGGAGCTGGT GGAGCGCTCT GCAGACCGGC CTGGCTGGTG TCTGATCAGA 
ACCACGGATC AGACGCCCCA GCAAGAGGGT TTGCTGCCCA TGAGCGCCCT CTGCCTGTCG 
CACTCCCACA GTGCAGGTGA CATGGAGGGG CTCCTCCCAG CCTCCACCAC CTCCTCTAGC 
ACCTCCTCCA CCTGCACCAC CACCTCCTCC TCTCTCAACT CCTCCTCCAC CACCACCTCC 
TCATCCACCA TGAGCGCAGC AAAGGATTCC AGTCTGTTCA GTTCTGAGGG TTCAGGCCTC 
CCGACTCAGA CCAGCAGTCA AAGTGCCACC TCCCCCACCG TAGGTGGACC GGGCGGAGCT 
CCAGTAGGTG CAGTAGGGTC TCCGGGGCCC AAACGGAGCG GCTCTGGCAC AGGCAACACG 
TTAAAGCGGT GGTTGACCAG CCCTGTGCGA AGGCTGAGTC AGGGAAAAGC TGACGGACAG 
AAAAAACCTC CAATCAGAGC CAGGAGGCGG GACAACAAGC TCGAGCTGGC CACGCCCCTT 
ACCGTAAATA CACAGATGAA GGCAAGAAAG GAGGAGGCGG GGCAAAGTGG AGGGGAGGAG 
GAGCAAGAGG AAGAAAGCCA CACCCCATTA CCTCCACCGA TGCAGATTAT GAAAGACCCC 
AACAATCCAG AGGCTCTGGA CTCAGATCAG GGATCAAACG AGTCCGATAC TGAACAGAGA 
AACAAAGCAC TAAGAGGACG AATGTTTGTT GTTAATGAGA TGGTCCAATC AGAGAAGGAC 
TACGTAAAGG ACCTAGGAGT GATTGTTGAG GGCTTCATGT CCAGGCTTGA GGTCAGGGGG 
ATCCCAGAAG ACATGAGGGG AAAAGACAAG ATCGTGTTTG GCAACATCCA GCAGATCTAC 
GACTGGCACC GCGACTTCTT TCTGGTGGAG CTAGAGCGCT GCATCCAGAA CCACGATCTG 
CTGGCCGACC TGTTCATCAG ACATGAGCGT CGTCTGCACA TGTATGTGGT TTACTGTCAA 
AACAAGCCCA AGTCGGAGTT CATCGTCATT GAGTATGAGA CCTTCTTTGA GGAGATCCAG 
CATGAGATCA GCTGCAGGAT GTCGATCAGT GATTATCTGA TCAAACCCAT CCAGAGAATC 
ACCAAGTACC AGCTACTACT GAAGGACTTC TTCAAATATA CGACCAAAGC AGGGCTGGAC 
ACTGAGGAGA TTGAGAAAGC GGTGGAGCTG ATGTCATTGG TTCCAAAGCG CTGCAACGAC 
ATGATGAACC TGGGACGCCT GCAGGGATAT GAGGGGAAGT TGACGTCTCA GGGGAAGCTG 
CTGCAGCAGG AGACCTTCTG CGTTTGGGAG CAGGACGGGG GAGTCTTGTC TCGCAGCAAA 
GAGCGCAGAG TCTTCCTCTT CGAGCAGATC ATCATCTTCA GTGAACTGCT CCGGAAAGGC 
TCCAACAATC CAGGATACCA GTTCAAGAAC AGCATCAAGG TGAGTTACCT GGCCATGCAG 
GACAGCGTAG ACGGCGATCC CTGTAAGTTT GTGCTGTGGT CCCGGGGCTC GGCAGAGCGC 
TTCACTCTAC AAGCGTCCTC CGCCAGCATC AAGATGACCT GGGTGGAGAC CATTGCCACT 
CTGCTGGACG CCCAGAACAA CTTCCTCTCT GCTCTCCAGT CACCTATTGA GTACCAGAGA 
AAAGAGGGCG GGATTTCAGT GACCCGTCCA CTGTCGTCTG GTCGTCCACC GTCTGCTCCG 
CCCACGCCTA ACGGCCATGC CACTCAACCT CCTGCTGACA GTGAGGATGA CCAGGAGCTG 
GTTCAGGTGC TGCAGGACTT TGTGGCCGTG CGGGAGGACG AGATTTCCGT GTTTCGGGGA 
GAGAAAGTCC AGATTCTGTC GTCCAACCAG CAGGGTCAGA GTCTTGTTTA CAGACCTGCC 
AACACCGACT CGCCCGCCGC CGAGGGCTGG GTGCCACGAA GCGTGCTCAA AACACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020563636.1
CDS	452..7531
Translation	MNPADGGGEAGPDGQATAGSGKGESVRAVDVLTVLREKVAFVSGGRDKRGGPILTFPARTNHDRIKQEDLSRLVTYLSTIPSEDVRARGFTVVVDMRGSKWDSVKPVLRTLQESFSSNIHTALLIKPDTFWQKHKTNLGSAKLSFETSLVSVEGLSRLIDPSQLTADLGGSLEYDHVEWTELRLGLEEFSGAATQLLTQLEQLEAGLSRIPEPQDIDEARKLLEEHTRLRNSIATAPVDALDREGKCLLQRMRLGSSHGEASNESGGGGGTWLSGGADFQSVAPKVSSLLDRLQGARSHLLQCWGDKKLHLDQALQLHLFEQDATKMSEWIAHSKELFMQSLAEVGQNHQHALDLQTQHQHFTANCMNGSVNVDSVVTVAARLAEAGHYGAERIALVSSKLQDNWKSMTSALEERSNMLAMATGFHQGAEQFLARVEGWVKLCSEGPLPSTTAELEPAIKKHQELNEEISANYTQVSESGKALLEVLQRCPTTDSDESATKPDFTAATHTIMGVLHQVMQGHHEVEGAWQHRKLRLHQRLQLCVFQQDVKQVLDWVEQHGEVFLNKHTGVGKSLHRARALQKRHDDFQQVAQNTYTNAEKLLEAADQLAQSGECEEEEIYQAARDLELRMQAFIQRVEQRKLLLDLAVSFYTHTKELSAWMEGLQKQLSSDLLVCPDTVEALQALISQQQQQQTNTQEAAMSVIREGEDLILQLRPQGSSVAHVESVLQQLEESHVQLEELFHQRKIRVDIFLQFRILHQCTLEVTAEMDAWKMDLEKQSQEFSSEKVASNRLAETNQDKLTQDKLALGQSRLVESSPEALTLAQQRIHRHMERRLAMNNMIYEVIQQGHDLQQYISEVQASGIELSDVEGGLSSQVEELQRLLQDKQKELQQIADHTHTLLEQNLQLRHLQSQVKQVLGWISEGEVMLSSCMLNSSCLSEAEQLQKEHERLQQAIEALLHADSLQRTHQVALALQQRAELLVRSGHYDPDAVRSCAQTVALHWQTLMLRIEDRLKLVNASAAFYRTSQQVCGVLESLEQEYRREEDWCAGGERQPEQLLPLINKHMEQKEAFLKACTLARRNAEVFLKYIHRNSVTMANISGHSITVSGVSEVTGHSRVPEQQVKGILSELLQRENRVLHFWTLKKRRLDQCQQYLMFQTHAQQALDWLQQSGDHYLATHTSPGATMAETQELLNQYREFCISAKVHTQEKVHLLIQLAESMLAKGHTHRTELRRCVSTVDKRYRDFSVRMGQYRHLLETALGGCSQDNKDLELELIPNSLSDSDPEVNLSDPSHQVSDEKRRSARKKEYIMAELLQTERVYVRDLQECIETYLWEMTSGSEDVPLGLANSHYTVFGNIQDIYEFHNSIFLKELENYEQLPEDVGHCFVTWADKFHMYVTYCRNKPDSSLLIQQHGVGFFEEVQRRHGLANSISSALIKPVQRITKYQLLLKELLACCEEGKGEIKEGLDVMLSVPKRANDAMHVSMLEGLEDGVEVQGELLLQDSFLVWEPKSLIRKGRDRHLFLFELSLIFSKEIKDSSGRTKYLYKSRLRTSELGVTEHIEGDQCKFALWVGRTPTSDNKTVLKASSLELKQEWVRSIRQVIQERRGHLRGALREPIPLPKTPNPTLGRQRSITRRETSEDADSLGDASSQPDTVSIASRTSQNTADSDKLSGGCELVVVLLDFTSGGPGELSVRCGQTVELVERSADRPGWCLIRTTDQTPQQEGLLPMSALCLSHSHSAGDMEGLLPASTTSSSTSSTCTTTSSSLNSSSTTTSSSTMSAAKDSSLFSSEGSGLPTQTSSQSATSPTVGGPGGAPVGAVGSPGPKRSGSGTGNTLKRWLTSPVRRLSQGKADGQKKPPIRARRRDNKLELATPLTVNTQMKARKEEAGQSGGEEEQEEESHTPLPPPMQIMKDPNNPEALDSDQGSNESDTEQRNKALRGRMFVVNEMVQSEKDYVKDLGVIVEGFMSRLEVRGIPEDMRGKDKIVFGNIQQIYDWHRDFFLVELERCIQNHDLLADLFIRHERRLHMYVVYCQNKPKSEFIVIEYETFFEEIQHEISCRMSISDYLIKPIQRITKYQLLLKDFFKYTTKAGLDTEEIEKAVELMSLVPKRCNDMMNLGRLQGYEGKLTSQGKLLQQETFCVWEQDGGVLSRSKERRVFLFEQIIIFSELLRKGSNNPGYQFKNSIKVSYLAMQDSVDGDPCKFVLWSRGSAERFTLQASSASIKMTWVETIATLLDAQNNFLSALQSPIEYQRKEGGISVTRPLSSGRPPSAPPTPNGHATQPPADSEDDQELVQVLQDFVAVREDEISVFRGEKVQILSSNQQGQSLVYRPANTDSPAAEGWVPRSVLKTH

Target ORF information:

RefSeq Version	XM_020707977.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes kalirin-like (LOC101166941), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020707977.1

ORF Insert Sequence:

ATGAATCCGG CAGATGGAGG AGGAGAAGCG GGCCCGGACG GCCAGGCGAC GGCAGGTTCT 
GGTAAAGGGG AGTCTGTCCG GGCGGTCGAT GTCTTGACGG TGCTCAGAGA AAAAGTGGCC 
TTTGTGTCGG GTGGACGCGA CAAACGAGGT GGACCAATTC TCACCTTTCC TGCTAGGACC 
AATCACGATC GAATAAAACA GGAAGACCTC AGCCGCTTGG TTACTTATTT ATCAACTATC 
CCTAGCGAGG ATGTGCGTGC CCGTGGCTTT ACCGTTGTGG TGGATATGCG GGGCAGTAAG 
TGGGACAGCG TGAAGCCGGT TCTGCGAACA CTACAGGAGT CCTTTTCCTC AAACATCCAC 
ACAGCACTAC TCATCAAACC GGACACCTTC TGGCAGAAAC ACAAGACCAA CCTAGGCAGT 
GCCAAGCTGA GTTTCGAGAC CAGTCTGGTG TCAGTGGAGG GTTTGTCCCG ACTTATTGAT 
CCCTCTCAGC TGACCGCTGA CCTCGGAGGT TCATTGGAGT ATGACCATGT GGAGTGGACT 
GAGCTGCGTC TTGGTTTAGA GGAGTTCTCA GGGGCTGCCA CACAGCTACT AACCCAACTG 
GAGCAGCTGG AAGCCGGGCT GAGCCGCATA CCCGAGCCTC AGGACATTGA TGAGGCCCGC 
AAACTCTTGG AAGAACACAC TCGCCTCCGC AATAGCATCG CCACTGCGCC TGTTGATGCC 
CTCGACCGAG AAGGGAAGTG TTTACTTCAG CGGATGCGCC TAGGTTCATC CCACGGTGAA 
GCCTCAAATG AGAGTGGGGG TGGTGGGGGA ACTTGGTTGT CAGGAGGAGC AGATTTCCAG 
AGCGTGGCGC CGAAGGTTTC GTCTTTATTG GACCGGCTGC AGGGGGCTCG CAGTCACTTA 
CTGCAGTGCT GGGGTGATAA GAAGCTGCAC TTGGACCAGG CACTGCAACT CCACCTGTTT 
GAACAGGATG CTACCAAGAT GTCAGAGTGG ATCGCTCACA GTAAAGAGCT CTTCATGCAG 
AGTCTGGCAG AAGTCGGTCA GAACCACCAG CACGCTCTCG ATCTACAAAC GCAGCACCAG 
CATTTCACCG CCAACTGCAT GAACGGCAGT GTGAATGTGG ACAGTGTCGT TACCGTGGCA 
GCAAGGCTCG CGGAGGCCGG CCACTACGGA GCAGAGCGGA TTGCTTTGGT GTCGTCAAAG 
TTACAGGACA ACTGGAAGTC AATGACAAGT GCTCTGGAAG AGCGGAGCAA CATGCTTGCC 
ATGGCAACCG GTTTCCATCA AGGGGCAGAG CAGTTTCTAG CCCGAGTGGA GGGATGGGTT 
AAACTGTGCT CTGAAGGGCC ATTGCCATCA ACAACAGCCG AGCTGGAACC TGCAATAAAA 
AAACACCAGG AGCTCAATGA GGAGATTTCA GCCAACTACA CACAGGTCAG TGAAAGCGGT 
AAGGCATTGC TGGAGGTCCT TCAGCGTTGC CCCACAACAG ATTCTGACGA ATCAGCAACC 
AAACCAGATT TCACTGCGGC CACACACACC ATTATGGGGG TTCTACACCA GGTGATGCAG 
GGTCATCACG AAGTAGAGGG AGCATGGCAG CATCGTAAGC TCCGCCTCCA CCAGCGCCTG 
CAGTTATGTG TGTTTCAACA GGATGTTAAA CAGGTTTTGG ACTGGGTGGA GCAGCATGGA 
GAGGTTTTCC TGAACAAACA CACGGGTGTG GGGAAGTCCT TGCACCGGGC ACGGGCGCTG 
CAGAAGCGAC ACGATGACTT TCAGCAGGTT GCACAGAATA CGTACACCAA TGCAGAGAAG 
CTTCTGGAGG CAGCAGACCA GTTGGCGCAG TCTGGAGAGT GTGAGGAAGA GGAGATCTAC 
CAGGCAGCCA GAGACCTGGA GCTGCGAATG CAGGCCTTCA TTCAGCGCGT TGAACAGAGG 
AAGCTGCTGC TGGACCTCGC CGTATCCTTC TACACGCACA CAAAGGAGCT CTCTGCATGG 
ATGGAGGGCC TCCAGAAGCA GCTCTCCTCA GACCTCCTCG TGTGCCCGGA CACTGTGGAA 
GCTCTGCAGG CTTTAATCAG CCAACAGCAG CAGCAGCAGA CCAACACTCA GGAAGCTGCG 
ATGAGTGTCA TCCGGGAAGG AGAAGACCTC ATCCTGCAAC TCAGGCCTCA GGGCAGCTCG 
GTGGCCCATG TGGAATCGGT TTTGCAGCAA CTGGAAGAGT CTCATGTTCA GCTTGAGGAA 
CTTTTTCACC AGAGGAAGAT CCGAGTGGAC ATTTTCCTGC AGTTCCGCAT ACTGCACCAG 
TGCACGCTGG AGGTTACAGC TGAGATGGAT GCTTGGAAAA TGGACTTGGA AAAACAATCC 
CAGGAATTTT CCTCGGAGAA GGTCGCCTCA AATCGGCTTG CTGAAACCAA CCAGGACAAG 
CTGACCCAGG ATAAGCTGGC ACTAGGACAG TCCCGGCTTG TGGAGTCCAG CCCGGAAGCG 
TTAACACTAG CACAGCAGCG GATTCACAGG CACATGGAAC GGAGGCTGGC GATGAACAAC 
ATGATATATG AGGTCATCCA GCAAGGTCAT GACCTTCAGC AGTACATCTC GGAGGTGCAG 
GCATCAGGTA TCGAGCTGTC AGACGTGGAG GGTGGGCTGT CCTCTCAGGT GGAGGAGCTG 
CAGCGCCTCC TGCAGGACAA ACAGAAGGAG CTGCAGCAGA TTGCCGATCA CACGCATACG 
CTGCTGGAGC AGAACCTGCA GCTGAGACAC CTCCAGAGCC AGGTCAAGCA GGTGCTCGGC 
TGGATCTCAG AGGGGGAGGT GATGCTGTCC TCCTGCATGT TGAATTCCAG CTGTCTGTCA 
GAGGCGGAGC AGCTGCAGAA GGAACACGAG CGCCTCCAAC AGGCCATAGA GGCTCTGCTG 
CACGCAGACT CCCTGCAGAG GACTCATCAG GTGGCGCTGG CCCTGCAGCA GAGAGCGGAG 
CTGCTGGTCA GGTCTGGCCA CTATGATCCA GATGCAGTGA GGTCCTGCGC TCAGACTGTA 
GCGCTGCATT GGCAGACACT AATGCTCAGG ATTGAAGACA GACTCAAACT GGTCAATGCG 
TCGGCTGCTT TCTACAGGAC ATCCCAGCAG GTATGTGGGG TCCTGGAGAG TCTGGAGCAG 
GAATACCGCA GGGAGGAGGA CTGGTGTGCA GGAGGAGAGA GGCAACCAGA GCAACTGCTG 
CCGCTAATCA ATAAACACAT GGAGCAGAAG GAGGCTTTTC TGAAGGCCTG TACTCTTGCG 
AGACGGAATG CAGAAGTTTT TCTCAAGTAT ATCCATCGCA ATAGCGTCAC CATGGCAAAT 
ATCTCTGGCC ATAGCATCAC TGTCTCTGGG GTCTCTGAGG TTACTGGCCA TTCCAGAGTC 
CCTGAACAAC AGGTCAAAGG TATCCTTAGT GAGCTGCTCC AGAGGGAAAA CAGAGTTCTT 
CATTTCTGGA CTTTAAAGAA ACGCAGATTG GATCAGTGCC AGCAGTATCT GATGTTCCAA 
ACCCACGCAC AGCAGGCTCT TGATTGGTTG CAGCAGTCAG GTGACCATTA CTTGGCCACA 
CATACGTCAC CAGGTGCAAC CATGGCTGAG ACACAGGAGC TGCTGAACCA ATACAGAGAG 
TTCTGCATTT CTGCAAAGGT GCACACACAG GAGAAGGTTC ACCTGCTGAT CCAGCTGGCT 
GAGAGTATGC TGGCTAAAGG TCACACCCAC CGCACTGAGC TCCGCCGCTG CGTTTCAACA 
GTGGATAAGC GCTACCGTGA CTTCTCTGTC AGGATGGGAC AATATCGCCA CCTGCTAGAG 
ACAGCGCTGG GAGGCTGTTC TCAGGACAAT AAAGACCTGG AGCTGGAGCT GATCCCCAAT 
AGTCTCTCGG ACTCTGACCC AGAGGTCAAC CTATCTGACC CAAGTCACCA AGTCAGTGAT 
GAGAAAAGAC GGTCCGCCCG CAAAAAGGAG TACATCATGG CAGAGCTCCT TCAGACAGAA 
CGAGTCTATG TCAGAGACCT GCAGGAGTGC ATAGAGACGT ACCTGTGGGA GATGACAAGC 
GGTTCAGAAG ATGTTCCACT CGGTCTTGCC AACAGTCACT ACACTGTGTT TGGAAACATT 
CAGGACATCT ACGAGTTCCA TAACAGTATT TTCTTAAAGG AGTTAGAGAA CTACGAGCAG 
CTTCCTGAAG ACGTGGGACA CTGTTTCGTC ACCTGGGCTG ATAAGTTCCA CATGTATGTG 
ACGTACTGCA GAAACAAACC AGACTCCAGT TTATTGATCC AACAGCATGG AGTGGGATTT 
TTTGAGGAGG TGCAGAGGAG ACACGGACTT GCTAACTCCA TCTCCTCTGC GCTGATTAAA 
CCGGTCCAGA GGATCACCAA GTACCAGCTG TTGCTTAAGG AGCTGCTGGC TTGCTGCGAG 
GAAGGCAAGG GGGAGATCAA GGAAGGCCTG GACGTGATGC TGAGTGTCCC AAAGAGAGCC 
AACGATGCAA TGCACGTCAG CATGTTAGAA GGTCTGGAGG ACGGGGTGGA GGTGCAGGGA 
GAGCTCCTCC TGCAGGACTC TTTCCTGGTC TGGGAGCCTA AGTCTCTGAT AAGAAAGGGG 
CGGGACAGAC ACTTATTCTT GTTTGAACTG TCACTCATCT TTAGCAAAGA GATTAAAGAC 
TCGTCCGGCC GCACCAAGTA TCTCTACAAA AGTCGCTTGA GGACGTCAGA GCTGGGAGTG 
ACGGAGCACA TAGAGGGGGA TCAGTGTAAG TTTGCACTGT GGGTGGGACG AACGCCGACA 
TCAGACAACA AGACCGTCCT GAAGGCTTCA TCCCTCGAGC TAAAGCAGGA GTGGGTTCGC 
AGTATCCGAC AGGTGATCCA GGAGAGGCGG GGTCACCTGC GAGGCGCACT GAGGGAGCCT 
ATCCCGCTCC CAAAAACCCC CAACCCCACG CTGGGACGAC AGCGCAGCAT CACCCGCAGG 
GAGACGAGCG AGGACGCAGA CAGCCTGGGT GATGCCAGCA GTCAGCCTGA CACGGTTTCC 
ATCGCATCAC GAACGTCGCA GAACACTGCC GACAGCGACA AGCTCTCTGG GGGCTGCGAG 
CTAGTGGTGG TGCTGCTGGA CTTCACCTCT GGAGGACCGG GAGAGCTCAG CGTCCGCTGC 
GGCCAGACGG TGGAGCTGGT GGAGCGCTCT GCAGACCGGC CTGGCTGGTG TCTGATCAGA 
ACCACGGATC AGACGCCCCA GCAAGAGGGT TTGCTGCCCA TGAGCGCCCT CTGCCTGTCG 
CACTCCCACA GTGCAGGTGA CATGGAGGGG CTCCTCCCAG CCTCCACCAC CTCCTCTAGC 
ACCTCCTCCA CCTGCACCAC CACCTCCTCC TCTCTCAACT CCTCCTCCAC CACCACCTCC 
TCATCCACCA TGAGCGCAGC AAAGGATTCC AGTCTGTTCA GTTCTGAGGG TTCAGGCCTC 
CCGACTCAGA CCAGCAGTCA AAGTGCCACC TCCCCCACCG TAGGTGGACC GGGCGGAGCT 
CCAGTAGGTG CAGTAGGGTC TCCGGGGCCC AAACGGAGCG GCTCTGGCAC AGGCAACACG 
TTAAAGCGGT GGTTGACCAG CCCTGTGCGA AGGCTGAGTC AGGGAAAAGC TGACGGACAG 
AAAAAACCTC CAATCAGAGC CAGGAGGCGG GACAACAAGC TCGAGCTGGC CACGCCCCTT 
ACCGTAAATA CACAGATGAA GGCAAGAAAG GAGGAGGCGG GGCAAAGTGG AGGGGAGGAG 
GAGCAAGAGG AAGAAAGCCA CACCCCATTA CCTCCACCGA TGCAGATTAT GAAAGACCCC 
AACAATCCAG AGGCTCTGGA CTCAGATCAG GGATCAAACG AGTCCGATAC TGAACAGAGA 
AACAAAGCAC TAAGAGGACG AATGTTTGTT GTTAATGAGA TGGTCCAATC AGAGAAGGAC 
TACGTAAAGG ACCTAGGAGT GATTGTTGAG GGCTTCATGT CCAGGCTTGA GGTCAGGGGG 
ATCCCAGAAG ACATGAGGGG AAAAGACAAG ATCGTGTTTG GCAACATCCA GCAGATCTAC 
GACTGGCACC GCGACTTCTT TCTGGTGGAG CTAGAGCGCT GCATCCAGAA CCACGATCTG 
CTGGCCGACC TGTTCATCAG ACATGAGCGT CGTCTGCACA TGTATGTGGT TTACTGTCAA 
AACAAGCCCA AGTCGGAGTT CATCGTCATT GAGTATGAGA CCTTCTTTGA GGAGATCCAG 
CATGAGATCA GCTGCAGGAT GTCGATCAGT GATTATCTGA TCAAACCCAT CCAGAGAATC 
ACCAAGTACC AGCTACTACT GAAGGACTTC TTCAAATATA CGACCAAAGC AGGGCTGGAC 
ACTGAGGAGA TTGAGAAAGC GGTGGAGCTG ATGTCATTGG TTCCAAAGCG CTGCAACGAC 
ATGATGAACC TGGGACGCCT GCAGGGATAT GAGGGGAAGT TGACGTCTCA GGGGAAGCTG 
CTGCAGCAGG AGACCTTCTG CGTTTGGGAG CAGGACGGGG GAGTCTTGTC TCGCAGCAAA 
GAGCGCAGAG TCTTCCTCTT CGAGCAGATC ATCATCTTCA GTGAACTGCT CCGGAAAGGC 
TCCAACAATC CAGGATACCA GTTCAAGAAC AGCATCAAGG TGAGTTACCT GGCCATGCAG 
GACAGCGTAG ACGGCGATCC CTGTAAGTTT GTGCTGTGGT CCCGGGGCTC GGCAGAGCGC 
TTCACTCTAC AAGCGTCCTC CGCCAGCATC AAGATGACCT GGGTGGAGAC CATTGCCACT 
CTGCTGGACG CCCAGAACAA CTTCCTCTCT GCTCTCCAGT CACCTATTGA GTACCAGAGA 
AAAGAGGGCG GGATTTCAGT GACCCGTCCA CTGTCGTCTG GTCGTCCACC GTCTGCTCCG 
CCCACGCCTA ACGGCCATGC CACTCAACCT CCTGCTGACA GTGAGGATGA CCAGGAGCTG 
GTTCAGGTGC TGCAGGACTT TGTGGCCGTG CGGGAGGACG AGATTTCCGT GTTTCGGGGA 
GAGAAAGTCC AGATTCTGTC GTCCAACCAG CAGGGTCAGA GTCTTGTTTA CAGACCTGCC 
AACACCGACT CGCCCGCCGC CGAGGGCTGG GTGCCACGAA GCGTGCTCAA AACACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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LOC101166941 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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