LOC101164837 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101164837
Entrez Gene ID	101164837
Full Name	trio Rho guanine nucleotide exchange factor
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020710033.1	XP_020565692.1	triple functional domain protein
XM_023964294.1	XP_023820062.1	triple functional domain protein isoform X1
XM_023964296.1	XP_023820064.1	triple functional domain protein isoform X3
XM_023964295.1	XP_023820063.1	triple functional domain protein isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC101164837 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101164837 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa109842	XM_023964295.1 Latest version!	Oryzias latipes triple functional domain protein (LOC101164837), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OJa109843	XM_023964296.1 Latest version!	Oryzias latipes triple functional domain protein (LOC101164837), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OJa72713	XM_023964294.1 Latest version!	Oryzias latipes triple functional domain protein (LOC101164837), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OJa72713	XM_020710033.1 Latest version!	Oryzias latipes trio Rho guanine nucleotide exchange factor (trio), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101164837 ( XM_023964295.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023820063.1 Oryzias latipes triple functional domain protein (LOC101164837), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OJa109842
Clone ID Related Accession (Same CDS sequence)	XM_023964295.1
Accession Version	XM_023964295.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	triple functional domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019874.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCAG GAGAGGGTCT GGATGAGGCT GCGAAGGACG CGGCAGACAT AGCGGCGTTT 
TTCAAATCCG ATTGCTCTCT TCCAAAGTGT GAATCCAAAC TGGACGGCCC CGGTCAAACA 
TCAGGGTTCC ATCGCAACGA AGATTTGAAA GCCATTGATG TGCTTCCCAT CCTAAAAGAG 
AAGGTCGCCT TTCTTTCAGG TGGCAGAGAC AGACGTGGGG GTCCAATTCT CACTTTTCCA 
GCACGGAGCA ACCATGACAG AATACGACCC GAGGACCTGC GTAGGCTTGT AGCATACTTG 
GCTACTATTC CAAGTGAGGA GGTGGCCAGA CATGGCTTCA CCGTCATCGT GGACATGCGT 
GGTTCTAAAT GGGACAGCAT CAAACCGTTG CTGAAAATCC TACAGGAGTC TTTTCCCACC 
TGCATCCACG TTGCCCTCAT CATCAAACCG GACAACTTCT GGCAAAAACA GAGAACCAAC 
TTTGGCAGTT CTAAGTTTGA ATTTGAAACG GTGATGGTCT CATTAGAGGG CTTAACCAAA 
ATAGTGGACC CATCGCAGCT GACAGCTGAC TTTGAGGGCA GCTTAGAGTA CAACCATGAC 
GACTGGATCG AGGTGCGGCT ATCATTCGAG ACATTTGCCA GTGATGCAGC GAGGGCTTTG 
TCACGCCTCG AGGAGCTCCA GGAGGTCCTG TCCCAGCGTG ATCTCCCGCG GGACCTGGAA 
GGAGCCCGCC GCCTCATGGA AGAGCACGCT GCGCTGAAGA AAAGGGCCAC AAAGGCGTCC 
ATAGAGGAGC TTGACACGCA GGGCCGACGG CTGCTCCAAA GACTGCAGTC TCATTCTGCA 
GGGGGTGGAA ACAGCAGTGA AAGCGGATAC GGCAACCGCG GCGTGGGGGC CAGTGGAGAT 
TCCAACGACC ATCACCACGG GCTCCAGGGT TACAGCGACG CACACAGTCT AGTGGCTAAA 
GTTACGGCTT TGTTGGATAA GCTGCATGGG ACGCGGCAGA ACTTGCAGCA GCTGTGGCAC 
ATGAGGAAGC TAAAACTCGA CCAGTGTTTC CAGCTGCGGC TGTTTGAGCA AGATGCTGAG 
AAGATGTTCG ACTGGATTAT GCACAACAAG GGGCTTTTTC TTACCAGCTA CACTGAGATT 
GGGGGGAACC ACCAGCATGC TGTTGAGCTG CAGACACAGC ACAATCATTT TGCTATGAAC 
TGCATGAATG TCTATGTGAA CATCAACCGA ATCATGTCGG TGGGAAACCG ACTCCTGGAG 
TCTGGTCACT ACGCCTCCCA GCAGATCCAG CAGATCTCCG GCCAGCTGGA GCAGGAATGG 
AAAGCCTTTG CTGCAGCCCT GGATGAACGC AGCACACTGC TGGAGATGTC TGCCAGCTTC 
CATCAGAAGG CAGATCAGTA CATGAGTAAA GTGGAGCCAT GGTGCAAGGC ATGTGGAGAG 
GGAGAGCTGC CCACCGAACT CCAGGACCTG GAGGATACAA TCCACCATCA CCAGGGACTC 
TACGAGCACA TCACCACGGC ATACTCAGAG GTAAGCCAGG AAGGCAAATC TTTACTGGAC 
AAGCTGCAGC GACCGTTGAC CCCAGGAAGT GCCGATTCGT TGATGTCCTC GGCCAACTAC 
TCCAAGGCTG TGCACCACGT CCTGGATATC ATCCACGAGG TTTTGCACCA TCAGAGACAA 
CTGGAAAACA TATGGCAGCA TCGCAAAGTC CGACTTCATC AGCGGCTGCA GCTTTGTGTG 
TTCCAGCAGG ACGTGCAGCA GGTTTTGGAT TGGATCGAAA ATCATGGCGA GGCTTTCCTA 
AGTAAGCACA CCGGCGTGGG GAAATCTCTG CACAGAGCCA GGGCTCTGCA GAAACGACAC 
GAGGACTTTG AAGAAGTTGC CCAGAACACC TACACTAATG CTGACAAGTT GCTGGAGGCA 
GCAGAGCAGC TGGCCCAAAC TGGAGAGTGT GACCCAGAGG AAATATACCA GGCCGCCCAC 
CAGCTAGAAG ACCGCATCCA AGATTTTGTG CGGCGTGTGG AGCAGCGTAA AGTCTTGCTG 
GACATGTCTG TTGCATTTCA CACGCATGTC AAAGAGCTGT GGACGTGGTT GGAGGAGCTG 
CAGAAGGAAC TGCTGGACGA TGTGTATGCG GAGTCCGTGG AGGCGGTTCA GGACTTGATC 
AAGCGCTTCG GCCAGCAGCA GCAGACCACG CTGCAGGCAA CCGTCAATGT TATCAAAGAG 
GGAGAGGACC TTATACAGCA GCTCAGAGAC TCGGCTATCT CCAGCAACAA GACCCCTCAC 
AACAGCTCCA TGGCCCACAT CGAGAGCGTG CTGCAGCAGC TGGATGAAGC CCAGGGCCAG 
ATGGAAGAGC TGTTCCAGGA AAGGAAGATC AAGCTGGAGC TGTTTCTCCA ACTACGCATA 
TTTGAGAGGG ATGCCATTGA TATCATCTCC GACCTGGAGT CATGGAACGA GGAACTGTCC 
CAGCAGATGA ACGACTTCGA TACAGAGGAC CTGACTCTGG CAGAGCAGAG GCTGCAGCAT 
CACGCTGACA AAGCGCTCAC CATGAATAAC CTGACCTTTG ACGTCATTCA CCAGGGGCAG 
GAGCTGCTTC AGTACGTCAC CGAGGTCCAG GCGTCCGGTG TGGAGCTGCT ATGCGACAGA 
GACGTGGACA TGGCCACACG GGTTCAGGAC CTGCTGGAGT TTCTTCATGA GAAGCAGCAG 
GAGCTGGACT TGGCTGCAGA CCAGCACAGG AGGCACTTGG AGCAGTGTGT CCAGTTAAGG 
CACCTGCAAG CTGAAGTTAA ACAGGTGCTG GGTTGGATCC GCAATGGGGA GTCGATGTTG 
AATGCAGGTC TAATAACGGC TAGCTCCTTG CAAGAAGCTG AGCAGCTGCA GAAGGAGCAC 
GAACAGTTCC AGCATGCAAT AGAGAAAACA CACCAAAGTG CTCTGCAGGT GCAGCAGAAG 
GCAGAAGCTC TTCTCCAGGC CAACCACTAC GACATGGACA TGATCAGAGA CTGTGCTGAA 
AAGGTGGCCG ACCACTGGCA GCAGCTGATG CTGAAGATGG AGGACAGACT TAAACTGGTC 
AATGCTTCTG TGGCCTTCTA TAAAACATCA GAACAGGTGT GCAGTGTGTT GGAAAGTCTG 
GAGCAGGAGT ACAAGCGGGA GGAGGACTGG TGTGGTGGGG CTGATAAACT GGGCCCAAAC 
AGTGAGTCAG ATCACGTCAC ACCTATGATC AGCAAACATC TGGAGCAGAA AGAAGCTTTT 
CTTAAGGCCT GCACATTAGC CAGACGCAAT GCTGATGTTT TCCTGAAGTA TCTGCACAGA 
AACAGCGTCA ACATGCCGGG CATGTTATCT CATGTCAAAG CACCAGAGAC TCAGGTCAAG 
AACATTTTGA ATGAGTTGCT TCAGAGAGAA AACAGAGTCC TCCACTTCTG GACGATGAGG 
AAGCGGAGGT TGGACCAGTG CCAGCAATAT GTAGTGTTTG AAAGAAGTGC CAAACAGGCC 
TTGGAATGGA TCCACGACAC CGGCGAGTTT TACCTGTCCA CTCATACATC CACTGGCTCC 
AGCATTCATC ACACACAGGA GCTACTGAAA GAGCACGAAG ACTTCCAGAT CACAGCAAAG 
CAAACCAAAG AGCGGGTAAA GCTGTTGATC CAGCTGGCGG ATGGGTTTTG TGACAAGGGC 
CACGCCCATG CCCTGGAAAT AAAGAAATGG GTTTCCTCGG TGGACAAGCG CTACAGAGAC 
TTCTCTATCC GTATGGACAA ATACCGAACC TGCCTGGAAA CGGCGCTGGG CATCTCGTCT 
GACTCAAACA AGGCTAGTAA AGGACTGCAG TTAGACATAA TCCCAGCTAG TGCTCCAGGG 
TCAGAGGTCA AGTTGAGGGA TGCCGCTCAT GAACTGAACG AGGAGAAGCG GAAATCTGCA 
CGACGAAAAG AATTTATAAT GGCTGAGCTG ATTCAAACAG AGAAAGCATA CGTGAGAGAT 
CTGCGAGAGT GCATGGATAC GTACCTGTGG GAAATGACCA GTGGTGTGGA GGAGATTCCC 
CATGGCATCA TCAACAAGGA ACACATCATC TTTGGGAACA TGCAGGACCT CTATGAGTTT 
CATCACAACA TTTTCCTCAA AGAGCTGGAG AAGTATGAGC AGCTTCCAGA GGATGTTGGC 
CATTGCTTTG TGACCTGGGC TGATAAGTTC CAGATGTATG TGAACTACTG TAAGAACAAG 
CCAGACTCAA CTCAACTCAT TGTTGAGCAT GCGGGAGCCT ACTTCGATGA AATTCAGCAA 
AGACATCGTC TTGCCAATTC CATCTCGTCC TACCTGATCA AGCCGGTTCA GCGAATCACC 
AAATACCAAC TGCTTCTCAA GGAGTTGCTA ACATGCTGTG AGGAGGGAAA AGGTGAAATA 
AAAGATGGTC TGGAAGTGAT GCTCAGTGTT CCCAAGAGAG CCAATGATGC CATGCATCTC 
TCAATGCTGG ACGGTTTCGA TGGGAACATT GACTCCCAAG GAGAGTTGAT CCTCCAAGAG 
TCCTTCCAGG TGTGGGACCC CAAGACTCTG ATTCGGAAAG GTCGTGAAAG GCACCTCTTC 
CTCTTTGAGA TGTCTCTCGT CTTCAGCAAA GAAGTAAAAG ATTCCAACGG ACACAGCAAA 
TACCTCTACA AGAGCAAGCT CTTTACCTCT GAATTGGGTG TGACAGAGCA TGTGGAGGGA 
GATCCTTGTA AGTTTGCCCT GTGGGTGGGC AGGACCCCAA CCTCAGACAA CAAGATTGTT 
CTTAAGGCCT CAAGTCTAGA GAATAAGCAG GACTGGATCA AACACATCAG AGAGGTCATC 
CAAGAGCGAA CCGTCCACCT GCGCGGAGCT CTGAAGGAGC CCATTCACAT CCCAAAAGCC 
ACAGCTACCA AACACAAGGG CAGACGGGAT GGAGAGGATC TGGACAGCCA AGGAGATGCC 
AGCAGCCAAC CAGACACCAT TTCCATCGCC TCTCGCACAT CTCAGAACAC GCTGGACAGT 
GACAAGCTCT CGGGCGGCTG TGAGCTGACG GTGGTAATCC ACGACTTTGT GGCCAGCAAT 
GGGGGTAGCA ACGGGGAACT AACAGTCCGG CGGGGCCAAA CTGTGGAGGT TCTGGAGCGG 
CTGCACGACA AGCCGGACTG GTGCCTGGTC CGAACCACAG ATCGGTCCCC TGCCCAGGAA 
GGCATAGTGC CCTGCTCCAT GCTCTGCATC GCCCACTCCC GCTCTTCCAT GGAGATGGAG 
GGGCTGTTCA ACCACAAAGA CACCTTGTCT GTATCGAGTA ATGACGCCCT GCAGCCAGGC 
TCCAGCCAAA CTTTGGGCCC CCACAGCTCA CCCGGACCCA AACGTCCCGG GAACACCTTA 
AGGAAATGGT TGACGAGCCC CGTACGCCGA CTCAGCTCGG GCAAAGCCGA TGGTCACATC 
AAAAAGCTGG CCCACAAGCA CAAAAAGAAC CGCGAAGGCA CCAGGAAGAA CATGGAAGTG 
ATCCCGGGGT CGCAGAAGGA CTCCGACGAC AGCGCCGCCA CTCCACAGGA CGAGACTTTG 
GAGGAACGCA TGCGCAACGA AGGGCTAAGC AGCGGCACCC TCTCAAAGTC TTCCTCATCG 
GGGATGCAGA GCTGCGGCGA GGAGGAGGGA GAGGAGGGTG CGGACGCTGT TCCTCTGCCT 
CCCCCGATGG CCATCCAGCA GCACAGCCTG CTGCAGCCTG ACTCCCAGGA TGACAAGTCT 
GCGTCTCGTC TGTCCGCGCG TCCTAGTAGT TCAGAGACAC CCAGTGCTGC AGAATTGGTC 
AGTGCCATCG AGGAGTTGGT GAAGAGCAAG ATGTCGCTGG AGGACCGGCC CAGTTCTCTG 
TCCGTGGAGC AAGGCGACAG CAGCTCACCC TCTCTCAACC CCTCCGACAA CTCACTCCTC 
TCCTCGTCCT CACCCACGGA CGAGATGGAC GAGCGCAAAT CTGGCTTCCT CAAGCGAAGG 
CACTATGTGC TCCTTGAGTT AGTGGAGACT GAAAGGGACT ACGTCAGGGA CCTTGGATCT 
GTTGTGGAGG GCTACATGAG CAGAATGAAA GAGGAGGGAG TTCCAGATGA CATGAGAGGA 
AAAGACAAGA TTGTTTTTGG AAACATCTAC CAGATCTACG ACTGGCACAA AGATTTTTTC 
CTGGGAGAGC TGGAGAAGTG TTTGGAAGAC CCCGAGAGGC TGGCTACTTT ATTTGTCAAA 
CAGGAGCGGA GGCTGCACAT GTACATCGTG TACTGCCAGA ACAAGCCCAA ATCCGAGCAC 
ATTGTGTCCG AGTATATTGA CACCTATTTT GAAGACCTAA AGCAGCGACT TGGTCACAGA 
CTGCAGCTCA CCGATTTGCT AATTAAACCA GTCCAGCGCA TAATGAAGTA CCAGCTGCTG 
CTAAAGGATC TACTCAAAGT TTCTAAAAAG GCAGGAGTGG ACACAGCAGA GCTGGAGAAA 
GCTGTTGAGG TGATGTGCGT GGTCCCAAAG CGTTGCAACG ACATGATGAA TGTTGGACGT 
CTTCAAGGCT TTGAAGGGAA AATTGTTGCT CAGGGCAGGC TGCTCCTCCA AGACACCTTC 
ATGGTTTCAG ACCAAGATGG GGGTCTACTG TCAAGGATGA AAGAAAGGAG AGTGTTTTTG 
TTTGAGCAGA TAGTTATATT CAGCGAACCT CTGGATAAAA AGAAGGGCTT CTCCACTCCT 
GGCTACCTCT TTAAAAACAG CATCAAGGTG AGCTGGCTGG GTCTGGAAGA AAACACAGAC 
GACCCCTGCA AGTTTACATT AACTTCAAGG TCATCTGCTG GGAGCATGGA GAGGTACACG 
CTTCACTCCG CCAGCCCAGG AGTGTGCCAA GTCTGGGTTC ACCAGGTCAG CCAGATCTTG 
GAAAATCAGC GGAACTTCCT CAACGCTTTG ACATCCCCCA TAGAGTACCA GAGGAACCAC 
GTGGGTGGAG GAGGGTCAAG CGGACCCCCT AGTAGTAGCA GCAGTAGTGC CGGAGGCCTC 
CCAGGAGGCA GCAGCACCAA CAATACCTCC AATTCGGGTG GAGGAGGAGG AGGTGGCGGA 
GGAGGAGGCT CAGGGGGTAA CTCCAATCTG TGCGGGCCTC GCTCTCGGCC CTCACGCATC 
CCGCAGCCTT CCTCGCGCCT CCCCCAGCCT GTTCACCACC ACCACCCCCC AGGCCCCGAG 
GGGCCTGACA GATCAGCAGC GCAACAAACG GAGAGCTGCC TGCCTCCGAG GTCCCTAAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023820063.1
CDS	226..7428
Translation	MSAGEGLDEAAKDAADIAAFFKSDCSLPKCESKLDGPGQTSGFHRNEDLKAIDVLPILKEKVAFLSGGRDRRGGPILTFPARSNHDRIRPEDLRRLVAYLATIPSEEVARHGFTVIVDMRGSKWDSIKPLLKILQESFPTCIHVALIIKPDNFWQKQRTNFGSSKFEFETVMVSLEGLTKIVDPSQLTADFEGSLEYNHDDWIEVRLSFETFASDAARALSRLEELQEVLSQRDLPRDLEGARRLMEEHAALKKRATKASIEELDTQGRRLLQRLQSHSAGGGNSSESGYGNRGVGASGDSNDHHHGLQGYSDAHSLVAKVTALLDKLHGTRQNLQQLWHMRKLKLDQCFQLRLFEQDAEKMFDWIMHNKGLFLTSYTEIGGNHQHAVELQTQHNHFAMNCMNVYVNINRIMSVGNRLLESGHYASQQIQQISGQLEQEWKAFAAALDERSTLLEMSASFHQKADQYMSKVEPWCKACGEGELPTELQDLEDTIHHHQGLYEHITTAYSEVSQEGKSLLDKLQRPLTPGSADSLMSSANYSKAVHHVLDIIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKVLLDMSVAFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQATVNVIKEGEDLIQQLRDSAISSNKTPHNSSMAHIESVLQQLDEAQGQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNEELSQQMNDFDTEDLTLAEQRLQHHADKALTMNNLTFDVIHQGQELLQYVTEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAADQHRRHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQKEHEQFQHAIEKTHQSALQVQQKAEALLQANHYDMDMIRDCAEKVADHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSESDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMLSHVKAPETQVKNILNELLQRENRVLHFWTMRKRRLDQCQQYVVFERSAKQALEWIHDTGEFYLSTHTSTGSSIHHTQELLKEHEDFQITAKQTKERVKLLIQLADGFCDKGHAHALEIKKWVSSVDKRYRDFSIRMDKYRTCLETALGISSDSNKASKGLQLDIIPASAPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPHGIINKEHIIFGNMQDLYEFHHNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVNYCKNKPDSTQLIVEHAGAYFDEIQQRHRLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLDGFDGNIDSQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSNGHSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSLENKQDWIKHIREVIQERTVHLRGALKEPIHIPKATATKHKGRRDGEDLDSQGDASSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFVASNGGSNGELTVRRGQTVEVLERLHDKPDWCLVRTTDRSPAQEGIVPCSMLCIAHSRSSMEMEGLFNHKDTLSVSSNDALQPGSSQTLGPHSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHIKKLAHKHKKNREGTRKNMEVIPGSQKDSDDSAATPQDETLEERMRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKSASRLSARPSSSETPSAAELVSAIEELVKSKMSLEDRPSSLSVEQGDSSSPSLNPSDNSLLSSSSPTDEMDERKSGFLKRRHYVLLELVETERDYVRDLGSVVEGYMSRMKEEGVPDDMRGKDKIVFGNIYQIYDWHKDFFLGELEKCLEDPERLATLFVKQERRLHMYIVYCQNKPKSEHIVSEYIDTYFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDLLKVSKKAGVDTAELEKAVEVMCVVPKRCNDMMNVGRLQGFEGKIVAQGRLLLQDTFMVSDQDGGLLSRMKERRVFLFEQIVIFSEPLDKKKGFSTPGYLFKNSIKVSWLGLEENTDDPCKFTLTSRSSAGSMERYTLHSASPGVCQVWVHQVSQILENQRNFLNALTSPIEYQRNHVGGGGSSGPPSSSSSSAGGLPGGSSTNNTSNSGGGGGGGGGGGSGGNSNLCGPRSRPSRIPQPSSRLPQPVHHHHPPGPEGPDRSAAQQTESCLPPRSLR

Target ORF information:

RefSeq Version	XM_023964295.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes triple functional domain protein (LOC101164837), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023964295.1

ORF Insert Sequence:

ATGAGCGCAG GAGAGGGTCT GGATGAGGCT GCGAAGGACG CGGCAGACAT AGCGGCGTTT 
TTCAAATCCG ATTGCTCTCT TCCAAAGTGT GAATCCAAAC TGGACGGCCC CGGTCAAACA 
TCAGGGTTCC ATCGCAACGA AGATTTGAAA GCCATTGATG TGCTTCCCAT CCTAAAAGAG 
AAGGTCGCCT TTCTTTCAGG TGGCAGAGAC AGACGTGGGG GTCCAATTCT CACTTTTCCA 
GCACGGAGCA ACCATGACAG AATACGACCC GAGGACCTGC GTAGGCTTGT AGCATACTTG 
GCTACTATTC CAAGTGAGGA GGTGGCCAGA CATGGCTTCA CCGTCATCGT GGACATGCGT 
GGTTCTAAAT GGGACAGCAT CAAACCGTTG CTGAAAATCC TACAGGAGTC TTTTCCCACC 
TGCATCCACG TTGCCCTCAT CATCAAACCG GACAACTTCT GGCAAAAACA GAGAACCAAC 
TTTGGCAGTT CTAAGTTTGA ATTTGAAACG GTGATGGTCT CATTAGAGGG CTTAACCAAA 
ATAGTGGACC CATCGCAGCT GACAGCTGAC TTTGAGGGCA GCTTAGAGTA CAACCATGAC 
GACTGGATCG AGGTGCGGCT ATCATTCGAG ACATTTGCCA GTGATGCAGC GAGGGCTTTG 
TCACGCCTCG AGGAGCTCCA GGAGGTCCTG TCCCAGCGTG ATCTCCCGCG GGACCTGGAA 
GGAGCCCGCC GCCTCATGGA AGAGCACGCT GCGCTGAAGA AAAGGGCCAC AAAGGCGTCC 
ATAGAGGAGC TTGACACGCA GGGCCGACGG CTGCTCCAAA GACTGCAGTC TCATTCTGCA 
GGGGGTGGAA ACAGCAGTGA AAGCGGATAC GGCAACCGCG GCGTGGGGGC CAGTGGAGAT 
TCCAACGACC ATCACCACGG GCTCCAGGGT TACAGCGACG CACACAGTCT AGTGGCTAAA 
GTTACGGCTT TGTTGGATAA GCTGCATGGG ACGCGGCAGA ACTTGCAGCA GCTGTGGCAC 
ATGAGGAAGC TAAAACTCGA CCAGTGTTTC CAGCTGCGGC TGTTTGAGCA AGATGCTGAG 
AAGATGTTCG ACTGGATTAT GCACAACAAG GGGCTTTTTC TTACCAGCTA CACTGAGATT 
GGGGGGAACC ACCAGCATGC TGTTGAGCTG CAGACACAGC ACAATCATTT TGCTATGAAC 
TGCATGAATG TCTATGTGAA CATCAACCGA ATCATGTCGG TGGGAAACCG ACTCCTGGAG 
TCTGGTCACT ACGCCTCCCA GCAGATCCAG CAGATCTCCG GCCAGCTGGA GCAGGAATGG 
AAAGCCTTTG CTGCAGCCCT GGATGAACGC AGCACACTGC TGGAGATGTC TGCCAGCTTC 
CATCAGAAGG CAGATCAGTA CATGAGTAAA GTGGAGCCAT GGTGCAAGGC ATGTGGAGAG 
GGAGAGCTGC CCACCGAACT CCAGGACCTG GAGGATACAA TCCACCATCA CCAGGGACTC 
TACGAGCACA TCACCACGGC ATACTCAGAG GTAAGCCAGG AAGGCAAATC TTTACTGGAC 
AAGCTGCAGC GACCGTTGAC CCCAGGAAGT GCCGATTCGT TGATGTCCTC GGCCAACTAC 
TCCAAGGCTG TGCACCACGT CCTGGATATC ATCCACGAGG TTTTGCACCA TCAGAGACAA 
CTGGAAAACA TATGGCAGCA TCGCAAAGTC CGACTTCATC AGCGGCTGCA GCTTTGTGTG 
TTCCAGCAGG ACGTGCAGCA GGTTTTGGAT TGGATCGAAA ATCATGGCGA GGCTTTCCTA 
AGTAAGCACA CCGGCGTGGG GAAATCTCTG CACAGAGCCA GGGCTCTGCA GAAACGACAC 
GAGGACTTTG AAGAAGTTGC CCAGAACACC TACACTAATG CTGACAAGTT GCTGGAGGCA 
GCAGAGCAGC TGGCCCAAAC TGGAGAGTGT GACCCAGAGG AAATATACCA GGCCGCCCAC 
CAGCTAGAAG ACCGCATCCA AGATTTTGTG CGGCGTGTGG AGCAGCGTAA AGTCTTGCTG 
GACATGTCTG TTGCATTTCA CACGCATGTC AAAGAGCTGT GGACGTGGTT GGAGGAGCTG 
CAGAAGGAAC TGCTGGACGA TGTGTATGCG GAGTCCGTGG AGGCGGTTCA GGACTTGATC 
AAGCGCTTCG GCCAGCAGCA GCAGACCACG CTGCAGGCAA CCGTCAATGT TATCAAAGAG 
GGAGAGGACC TTATACAGCA GCTCAGAGAC TCGGCTATCT CCAGCAACAA GACCCCTCAC 
AACAGCTCCA TGGCCCACAT CGAGAGCGTG CTGCAGCAGC TGGATGAAGC CCAGGGCCAG 
ATGGAAGAGC TGTTCCAGGA AAGGAAGATC AAGCTGGAGC TGTTTCTCCA ACTACGCATA 
TTTGAGAGGG ATGCCATTGA TATCATCTCC GACCTGGAGT CATGGAACGA GGAACTGTCC 
CAGCAGATGA ACGACTTCGA TACAGAGGAC CTGACTCTGG CAGAGCAGAG GCTGCAGCAT 
CACGCTGACA AAGCGCTCAC CATGAATAAC CTGACCTTTG ACGTCATTCA CCAGGGGCAG 
GAGCTGCTTC AGTACGTCAC CGAGGTCCAG GCGTCCGGTG TGGAGCTGCT ATGCGACAGA 
GACGTGGACA TGGCCACACG GGTTCAGGAC CTGCTGGAGT TTCTTCATGA GAAGCAGCAG 
GAGCTGGACT TGGCTGCAGA CCAGCACAGG AGGCACTTGG AGCAGTGTGT CCAGTTAAGG 
CACCTGCAAG CTGAAGTTAA ACAGGTGCTG GGTTGGATCC GCAATGGGGA GTCGATGTTG 
AATGCAGGTC TAATAACGGC TAGCTCCTTG CAAGAAGCTG AGCAGCTGCA GAAGGAGCAC 
GAACAGTTCC AGCATGCAAT AGAGAAAACA CACCAAAGTG CTCTGCAGGT GCAGCAGAAG 
GCAGAAGCTC TTCTCCAGGC CAACCACTAC GACATGGACA TGATCAGAGA CTGTGCTGAA 
AAGGTGGCCG ACCACTGGCA GCAGCTGATG CTGAAGATGG AGGACAGACT TAAACTGGTC 
AATGCTTCTG TGGCCTTCTA TAAAACATCA GAACAGGTGT GCAGTGTGTT GGAAAGTCTG 
GAGCAGGAGT ACAAGCGGGA GGAGGACTGG TGTGGTGGGG CTGATAAACT GGGCCCAAAC 
AGTGAGTCAG ATCACGTCAC ACCTATGATC AGCAAACATC TGGAGCAGAA AGAAGCTTTT 
CTTAAGGCCT GCACATTAGC CAGACGCAAT GCTGATGTTT TCCTGAAGTA TCTGCACAGA 
AACAGCGTCA ACATGCCGGG CATGTTATCT CATGTCAAAG CACCAGAGAC TCAGGTCAAG 
AACATTTTGA ATGAGTTGCT TCAGAGAGAA AACAGAGTCC TCCACTTCTG GACGATGAGG 
AAGCGGAGGT TGGACCAGTG CCAGCAATAT GTAGTGTTTG AAAGAAGTGC CAAACAGGCC 
TTGGAATGGA TCCACGACAC CGGCGAGTTT TACCTGTCCA CTCATACATC CACTGGCTCC 
AGCATTCATC ACACACAGGA GCTACTGAAA GAGCACGAAG ACTTCCAGAT CACAGCAAAG 
CAAACCAAAG AGCGGGTAAA GCTGTTGATC CAGCTGGCGG ATGGGTTTTG TGACAAGGGC 
CACGCCCATG CCCTGGAAAT AAAGAAATGG GTTTCCTCGG TGGACAAGCG CTACAGAGAC 
TTCTCTATCC GTATGGACAA ATACCGAACC TGCCTGGAAA CGGCGCTGGG CATCTCGTCT 
GACTCAAACA AGGCTAGTAA AGGACTGCAG TTAGACATAA TCCCAGCTAG TGCTCCAGGG 
TCAGAGGTCA AGTTGAGGGA TGCCGCTCAT GAACTGAACG AGGAGAAGCG GAAATCTGCA 
CGACGAAAAG AATTTATAAT GGCTGAGCTG ATTCAAACAG AGAAAGCATA CGTGAGAGAT 
CTGCGAGAGT GCATGGATAC GTACCTGTGG GAAATGACCA GTGGTGTGGA GGAGATTCCC 
CATGGCATCA TCAACAAGGA ACACATCATC TTTGGGAACA TGCAGGACCT CTATGAGTTT 
CATCACAACA TTTTCCTCAA AGAGCTGGAG AAGTATGAGC AGCTTCCAGA GGATGTTGGC 
CATTGCTTTG TGACCTGGGC TGATAAGTTC CAGATGTATG TGAACTACTG TAAGAACAAG 
CCAGACTCAA CTCAACTCAT TGTTGAGCAT GCGGGAGCCT ACTTCGATGA AATTCAGCAA 
AGACATCGTC TTGCCAATTC CATCTCGTCC TACCTGATCA AGCCGGTTCA GCGAATCACC 
AAATACCAAC TGCTTCTCAA GGAGTTGCTA ACATGCTGTG AGGAGGGAAA AGGTGAAATA 
AAAGATGGTC TGGAAGTGAT GCTCAGTGTT CCCAAGAGAG CCAATGATGC CATGCATCTC 
TCAATGCTGG ACGGTTTCGA TGGGAACATT GACTCCCAAG GAGAGTTGAT CCTCCAAGAG 
TCCTTCCAGG TGTGGGACCC CAAGACTCTG ATTCGGAAAG GTCGTGAAAG GCACCTCTTC 
CTCTTTGAGA TGTCTCTCGT CTTCAGCAAA GAAGTAAAAG ATTCCAACGG ACACAGCAAA 
TACCTCTACA AGAGCAAGCT CTTTACCTCT GAATTGGGTG TGACAGAGCA TGTGGAGGGA 
GATCCTTGTA AGTTTGCCCT GTGGGTGGGC AGGACCCCAA CCTCAGACAA CAAGATTGTT 
CTTAAGGCCT CAAGTCTAGA GAATAAGCAG GACTGGATCA AACACATCAG AGAGGTCATC 
CAAGAGCGAA CCGTCCACCT GCGCGGAGCT CTGAAGGAGC CCATTCACAT CCCAAAAGCC 
ACAGCTACCA AACACAAGGG CAGACGGGAT GGAGAGGATC TGGACAGCCA AGGAGATGCC 
AGCAGCCAAC CAGACACCAT TTCCATCGCC TCTCGCACAT CTCAGAACAC GCTGGACAGT 
GACAAGCTCT CGGGCGGCTG TGAGCTGACG GTGGTAATCC ACGACTTTGT GGCCAGCAAT 
GGGGGTAGCA ACGGGGAACT AACAGTCCGG CGGGGCCAAA CTGTGGAGGT TCTGGAGCGG 
CTGCACGACA AGCCGGACTG GTGCCTGGTC CGAACCACAG ATCGGTCCCC TGCCCAGGAA 
GGCATAGTGC CCTGCTCCAT GCTCTGCATC GCCCACTCCC GCTCTTCCAT GGAGATGGAG 
GGGCTGTTCA ACCACAAAGA CACCTTGTCT GTATCGAGTA ATGACGCCCT GCAGCCAGGC 
TCCAGCCAAA CTTTGGGCCC CCACAGCTCA CCCGGACCCA AACGTCCCGG GAACACCTTA 
AGGAAATGGT TGACGAGCCC CGTACGCCGA CTCAGCTCGG GCAAAGCCGA TGGTCACATC 
AAAAAGCTGG CCCACAAGCA CAAAAAGAAC CGCGAAGGCA CCAGGAAGAA CATGGAAGTG 
ATCCCGGGGT CGCAGAAGGA CTCCGACGAC AGCGCCGCCA CTCCACAGGA CGAGACTTTG 
GAGGAACGCA TGCGCAACGA AGGGCTAAGC AGCGGCACCC TCTCAAAGTC TTCCTCATCG 
GGGATGCAGA GCTGCGGCGA GGAGGAGGGA GAGGAGGGTG CGGACGCTGT TCCTCTGCCT 
CCCCCGATGG CCATCCAGCA GCACAGCCTG CTGCAGCCTG ACTCCCAGGA TGACAAGTCT 
GCGTCTCGTC TGTCCGCGCG TCCTAGTAGT TCAGAGACAC CCAGTGCTGC AGAATTGGTC 
AGTGCCATCG AGGAGTTGGT GAAGAGCAAG ATGTCGCTGG AGGACCGGCC CAGTTCTCTG 
TCCGTGGAGC AAGGCGACAG CAGCTCACCC TCTCTCAACC CCTCCGACAA CTCACTCCTC 
TCCTCGTCCT CACCCACGGA CGAGATGGAC GAGCGCAAAT CTGGCTTCCT CAAGCGAAGG 
CACTATGTGC TCCTTGAGTT AGTGGAGACT GAAAGGGACT ACGTCAGGGA CCTTGGATCT 
GTTGTGGAGG GCTACATGAG CAGAATGAAA GAGGAGGGAG TTCCAGATGA CATGAGAGGA 
AAAGACAAGA TTGTTTTTGG AAACATCTAC CAGATCTACG ACTGGCACAA AGATTTTTTC 
CTGGGAGAGC TGGAGAAGTG TTTGGAAGAC CCCGAGAGGC TGGCTACTTT ATTTGTCAAA 
CAGGAGCGGA GGCTGCACAT GTACATCGTG TACTGCCAGA ACAAGCCCAA ATCCGAGCAC 
ATTGTGTCCG AGTATATTGA CACCTATTTT GAAGACCTAA AGCAGCGACT TGGTCACAGA 
CTGCAGCTCA CCGATTTGCT AATTAAACCA GTCCAGCGCA TAATGAAGTA CCAGCTGCTG 
CTAAAGGATC TACTCAAAGT TTCTAAAAAG GCAGGAGTGG ACACAGCAGA GCTGGAGAAA 
GCTGTTGAGG TGATGTGCGT GGTCCCAAAG CGTTGCAACG ACATGATGAA TGTTGGACGT 
CTTCAAGGCT TTGAAGGGAA AATTGTTGCT CAGGGCAGGC TGCTCCTCCA AGACACCTTC 
ATGGTTTCAG ACCAAGATGG GGGTCTACTG TCAAGGATGA AAGAAAGGAG AGTGTTTTTG 
TTTGAGCAGA TAGTTATATT CAGCGAACCT CTGGATAAAA AGAAGGGCTT CTCCACTCCT 
GGCTACCTCT TTAAAAACAG CATCAAGGTG AGCTGGCTGG GTCTGGAAGA AAACACAGAC 
GACCCCTGCA AGTTTACATT AACTTCAAGG TCATCTGCTG GGAGCATGGA GAGGTACACG 
CTTCACTCCG CCAGCCCAGG AGTGTGCCAA GTCTGGGTTC ACCAGGTCAG CCAGATCTTG 
GAAAATCAGC GGAACTTCCT CAACGCTTTG ACATCCCCCA TAGAGTACCA GAGGAACCAC 
GTGGGTGGAG GAGGGTCAAG CGGACCCCCT AGTAGTAGCA GCAGTAGTGC CGGAGGCCTC 
CCAGGAGGCA GCAGCACCAA CAATACCTCC AATTCGGGTG GAGGAGGAGG AGGTGGCGGA 
GGAGGAGGCT CAGGGGGTAA CTCCAATCTG TGCGGGCCTC GCTCTCGGCC CTCACGCATC 
CCGCAGCCTT CCTCGCGCCT CCCCCAGCCT GTTCACCACC ACCACCCCCC AGGCCCCGAG 
GGGCCTGACA GATCAGCAGC GCAACAAACG GAGAGCTGCC TGCCTCCGAG GTCCCTAAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101164837 ( XM_023964296.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023820064.1 Oryzias latipes triple functional domain protein (LOC101164837), transcript variant X3, mRNA.

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CloneID	OJa109843
Clone ID Related Accession (Same CDS sequence)	XM_023964296.1
Accession Version	XM_023964296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	triple functional domain protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019874.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCAG GAGAGGGTCT GGATGAGGCT GCGAAGGACG CGGCAGACAT AGCGGCGTTT 
TTCAAATCCG ATTGCTCTCT TCCAAAGTGT GAATCCAAAC TGGACGGCCC CGGTCAAACA 
TCAGGGTTCC ATCGCAACGA AGATTTGAAA GCCATTGATG TGCTTCCCAT CCTAAAAGAG 
AAGGTCGCCT TTCTTTCAGG TGGCAGAGAC AGACGTGGGG GTCCAATTCT CACTTTTCCA 
GCACGGAGCA ACCATGACAG AATACGACCC GAGGACCTGC GTAGGCTTGT AGCATACTTG 
GCTACTATTC CAAGTGAGGA GGTGGCCAGA CATGGCTTCA CCGTCATCGT GGACATGCGT 
GGTTCTAAAT GGGACAGCAT CAAACCGTTG CTGAAAATCC TACAGGAGTC TTTTCCCACC 
TGCATCCACG TTGCCCTCAT CATCAAACCG GACAACTTCT GGCAAAAACA GAGAACCAAC 
TTTGGCAGTT CTAAGTTTGA ATTTGAAACG GTGATGGTCT CATTAGAGGG CTTAACCAAA 
ATAGTGGACC CATCGCAGCT GACAGCTGAC TTTGAGGGCA GCTTAGAGTA CAACCATGAC 
GACTGGATCG AGGTGCGGCT ATCATTCGAG ACATTTGCCA GTGATGCAGC GAGGGCTTTG 
TCACGCCTCG AGGAGCTCCA GGAGGTCCTG TCCCAGCGTG ATCTCCCGCG GGACCTGGAA 
GGAGCCCGCC GCCTCATGGA AGAGCACGCT GCGCTGAAGA AAAGGGCCAC AAAGGCGTCC 
ATAGAGGAGC TTGACACGCA GGGCCGACGG CTGCTCCAAA GACTGCAGTC TCATTCTGCA 
GGGGGTGGAA ACAGCAGTGA AAGCGGATAC GGCAACCGCG GCGTGGGGGC CAGTGGAGAT 
TCCAACGACC ATCACCACGG GCTCCAGGGT TACAGCGACG CACACAGTCT AGTGGCTAAA 
GTTACGGCTT TGTTGGATAA GCTGCATGGG ACGCGGCAGA ACTTGCAGCA GCTGTGGCAC 
ATGAGGAAGC TAAAACTCGA CCAGTGTTTC CAGCTGCGGC TGTTTGAGCA AGATGCTGAG 
AAGATGTTCG ACTGGATTAT GCACAACAAG GGGCTTTTTC TTACCAGCTA CACTGAGATT 
GGGGGGAACC ACCAGCATGC TGTTGAGCTG CAGACACAGC ACAATCATTT TGCTATGAAC 
TGCATGAATG TCTATGTGAA CATCAACCGA ATCATGTCGG TGGGAAACCG ACTCCTGGAG 
TCTGGTCACT ACGCCTCCCA GCAGATCCAG CAGATCTCCG GCCAGCTGGA GCAGGAATGG 
AAAGCCTTTG CTGCAGCCCT GGATGAACGC AGCACACTGC TGGAGATGTC TGCCAGCTTC 
CATCAGAAGG CAGATCAGTA CATGAGTAAA GTGGAGCCAT GGTGCAAGGC ATGTGGAGAG 
GGAGAGCTGC CCACCGAACT CCAGGACCTG GAGGATACAA TCCACCATCA CCAGGGACTC 
TACGAGCACA TCACCACGGC ATACTCAGAG GTAAGCCAGG AAGGCAAATC TTTACTGGAC 
AAGCTGCAGC GACCGTTGAC CCCAGGAAGT GCCGATTCGT TGATGTCCTC GGCCAACTAC 
TCCAAGGCTG TGCACCACGT CCTGGATATC ATCCACGAGG TTTTGCACCA TCAGAGACAA 
CTGGAAAACA TATGGCAGCA TCGCAAAGTC CGACTTCATC AGCGGCTGCA GCTTTGTGTG 
TTCCAGCAGG ACGTGCAGCA GGTTTTGGAT TGGATCGAAA ATCATGGCGA GGCTTTCCTA 
AGTAAGCACA CCGGCGTGGG GAAATCTCTG CACAGAGCCA GGGCTCTGCA GAAACGACAC 
GAGGACTTTG AAGAAGTTGC CCAGAACACC TACACTAATG CTGACAAGTT GCTGGAGGCA 
GCAGAGCAGC TGGCCCAAAC TGGAGAGTGT GACCCAGAGG AAATATACCA GGCCGCCCAC 
CAGCTAGAAG ACCGCATCCA AGATTTTGTG CGGCGTGTGG AGCAGCGTAA AGTCTTGCTG 
GACATGTCTG TTGCATTTCA CACGCATGTC AAAGAGCTGT GGACGTGGTT GGAGGAGCTG 
CAGAAGGAAC TGCTGGACGA TGTGTATGCG GAGTCCGTGG AGGCGGTTCA GGACTTGATC 
AAGCGCTTCG GCCAGCAGCA GCAGACCACG CTGCAGGCAA CCGTCAATGT TATCAAAGAG 
GGAGAGGACC TTATACAGCA GCTCAGAGAC TCGGCTATCT CCAGCAACAA GACCCCTCAC 
AACAGCTCCA TGGCCCACAT CGAGAGCGTG CTGCAGCAGC TGGATGAAGC CCAGGGCCAG 
ATGGAAGAGC TGTTCCAGGA AAGGAAGATC AAGCTGGAGC TGTTTCTCCA ACTACGCATA 
TTTGAGAGGG ATGCCATTGA TATCATCTCC GACCTGGAGT CATGGAACGA GGAACTGTCC 
CAGCAGATGA ACGACTTCGA TACAGAGGAC CTGACTCTGG CAGAGCAGAG GCTGCAGCAT 
CACGCTGACA AAGCGCTCAC CATGAATAAC CTGACCTTTG ACGTCATTCA CCAGGGGCAG 
GAGCTGCTTC AGTACGTCAC CGAGGTCCAG GCGTCCGGTG TGGAGCTGCT ATGCGACAGA 
GACGTGGACA TGGCCACACG GGTTCAGGAC CTGCTGGAGT TTCTTCATGA GAAGCAGCAG 
GAGCTGGACT TGGCTGCAGA CCAGCACAGG AGGCACTTGG AGCAGTGTGT CCAGTTAAGG 
CACCTGCAAG CTGAAGTTAA ACAGGTGCTG GGTTGGATCC GCAATGGGGA GTCGATGTTG 
AATGCAGGTC TAATAACGGC TAGCTCCTTG CAAGAAGCTG AGCAGCTGCA GAAGGAGCAC 
GAACAGTTCC AGCATGCAAT AGAGAAAACA CACCAAAGTG CTCTGCAGGT GCAGCAGAAG 
GCAGAAGCTC TTCTCCAGGC CAACCACTAC GACATGGACA TGATCAGAGA CTGTGCTGAA 
AAGGTGGCCG ACCACTGGCA GCAGCTGATG CTGAAGATGG AGGACAGACT TAAACTGGTC 
AATGCTTCTG TGGCCTTCTA TAAAACATCA GAACAGGTGT GCAGTGTGTT GGAAAGTCTG 
GAGCAGGAGT ACAAGCGGGA GGAGGACTGG TGTGGTGGGG CTGATAAACT GGGCCCAAAC 
AGTGAGTCAG ATCACGTCAC ACCTATGATC AGCAAACATC TGGAGCAGAA AGAAGCTTTT 
CTTAAGGCCT GCACATTAGC CAGACGCAAT GCTGATGTTT TCCTGAAGTA TCTGCACAGA 
AACAGCGTCA ACATGCCGGG CATGTTATCT CATGTCAAAG CACCAGAGAC TCAGGTCAAG 
AACATTTTGA ATGAGTTGCT TCAGAGAGAA AACAGAGTCC TCCACTTCTG GACGATGAGG 
AAGCGGAGGT TGGACCAGTG CCAGCAATAT GTAGTGTTTG AAAGAAGTGC CAAACAGGCC 
TTGGAATGGA TCCACGACAC CGGCGAGTTT TACCTGTCCA CTCATACATC CACTGGCTCC 
AGCATTCATC ACACACAGGA GCTACTGAAA GAGCACGAAG ACTTCCAGAT CACAGCAAAG 
CAAACCAAAG AGCGGGTAAA GCTGTTGATC CAGCTGGCGG ATGGGTTTTG TGACAAGGGC 
CACGCCCATG CCCTGGAAAT AAAGAAATGG GTTTCCTCGG TGGACAAGCG CTACAGAGAC 
TTCTCTATCC GTATGGACAA ATACCGAACC TGCCTGGAAA CGGCGCTGGG CATCTCGTCT 
GACTCAAACA AGGCTAGTAA AGGACTGCAG TTAGACATAA TCCCAGCTAG TGCTCCAGGG 
TCAGAGGTCA AGTTGAGGGA TGCCGCTCAT GAACTGAACG AGGAGAAGCG GAAATCTGCA 
CGACGAAAAG AATTTATAAT GGCTGAGCTG ATTCAAACAG AGAAAGCATA CGTGAGAGAT 
CTGCGAGAGT GCATGGATAC GTACCTGTGG GAAATGACCA GTGGTGTGGA GGAGATTCCC 
CATGGCATCA TCAACAAGGA ACACATCATC TTTGGGAACA TGCAGGACCT CTATGAGTTT 
CATCACAACA TTTTCCTCAA AGAGCTGGAG AAGTATGAGC AGCTTCCAGA GGATGTTGGC 
CATTGCTTTG TGACCTGGGC TGATAAGTTC CAGATGTATG TGAACTACTG TAAGAACAAG 
CCAGACTCAA CTCAACTCAT TGTTGAGCAT GCGGGAGCCT ACTTCGATGA AATTCAGCAA 
AGACATCGTC TTGCCAATTC CATCTCGTCC TACCTGATCA AGCCGGTTCA GCGAATCACC 
AAATACCAAC TGCTTCTCAA GGAGTTGCTA ACATGCTGTG AGGAGGGAAA AGGTGAAATA 
AAAGATGGTC TGGAAGTGAT GCTCAGTGTT CCCAAGAGAG CCAATGATGC CATGCATCTC 
TCAATGCTGG ACGGTTTCGA TGGGAACATT GACTCCCAAG GAGAGTTGAT CCTCCAAGAG 
TCCTTCCAGG TGTGGGACCC CAAGACTCTG ATTCGGAAAG GTCGTGAAAG GCACCTCTTC 
CTCTTTGAGA TGTCTCTCGT CTTCAGCAAA GAAGTAAAAG ATTCCAACGG ACACAGCAAA 
TACCTCTACA AGAGCAAGCT CTTTACCTCT GAATTGGGTG TGACAGAGCA TGTGGAGGGA 
GATCCTTGTA AGTTTGCCCT GTGGGTGGGC AGGACCCCAA CCTCAGACAA CAAGATTGTT 
CTTAAGGCCT CAAGTCTAGA GAATAAGCAG GACTGGATCA AACACATCAG AGAGGTCATC 
CAAGAGCGAA CCGTCCACCT GCGCGGAGCT CTGAAGGAGC CCATTCACAT CCCAAAAGCC 
ACAGCTACCA AACACAAGGG CAGACGGGAT GGAGAGGATC TGGACAGCCA AGGAGATGCC 
AGCAGCCAAC CAGACACCAT TTCCATCGCC TCTCGCACAT CTCAGAACAC GCTGGACAGT 
GACAAGCTCT CGGGCGGCTG TGAGCTGACG GTGGTAATCC ACGACTTTGT GGCCAGCAAT 
GGGGGTAGCA ACGGGGAACT AACAGTCCGG CGGGGCCAAA CTGTGGAGGT TCTGGAGCGG 
CTGCACGACA AGCCGGACTG GTGCCTGGTC CGAACCACAG ATCGGTCCCC TGCCCAGGAA 
GGCATAGTGC CCTGCTCCAT GCTCTGCATC GCCCACTCCC GCTCTTCCAT GGAGATGGAG 
GGGCTGTTCA ACCACAAAGA CACCTTGTCT GTATCGAGTA ATGACGCCCT GCAGCCAGGC 
TCCAGCCAAA CTTTGGGCCC CCACAGCTCA CCCGGACCCA AACGTCCCGG GAACACCTTA 
AGGAAATGGT TGACGAGCCC CGTACGCCGA CTCAGCTCGG GCAAAGCCGA TGGTCACATC 
AAAAAGCTGG CCCACAAGCA CAAAAAGAAC CGCGAAGGCA CCAGGAAGAA CATGGAAGTG 
ATCCCGGGGT CGCAGAAGGA CTCCGACGAC AGCGCCGCCA CTCCACAGGA CGAGACTTTG 
GAGGAACGCA TGCGCAACGA AGGGCTAAGC AGCGGCACCC TCTCAAAGTC TTCCTCATCG 
GGGATGCAGA GCTGCGGCGA GGAGGAGGGA GAGGAGGGTG CGGACGCTGT TCCTCTGCCT 
CCCCCGATGG CCATCCAGCA GCACAGCCTG CTGCAGCCTG ACTCCCAGGA TGACAAGTCT 
GCGTCTCGTC TGTCCGCGCG TCCTAGTAGT TCAGAGACAC CCAGTGCTGC AGAATTGGTC 
AGTGCCATCG AGGAGTTGGT GAAGAGCAAG ATGTCGCTGG AGGACCGGCC CAGTTCTCTG 
TCCGTGGAGC AAGGCGACAG CAGCTCACCC TCTCTCAACC CCTCCGACAA CTCACTCCTC 
TCCTCGTCCT CACCCACGGA CGAGATGGAC GAGCGCAAAT CTGGCTTCCT CAAGCGAAGG 
CACTATGTGC TCCTTGAGTT AGTGGAGACT GAAAGGGACT ACGTCAGGGA CCTTGGATCT 
GTTGTGGAGG GCTACATGAG CAGAATGAAA GAGGAGGGAG TTCCAGATGA CATGAGAGGA 
AAAGACAAGA TTGTTTTTGG AAACATCTAC CAGATCTACG ACTGGCACAA AGATTTTTTC 
CTGGGAGAGC TGGAGAAGTG TTTGGAAGAC CCCGAGAGGC TGGCTACTTT ATTTGTCAAA 
CAGGAGCGGA GGCTGCACAT GTACATCGTG TACTGCCAGA ACAAGCCCAA ATCCGAGCAC 
ATTGTGTCCG AGTATATTGA CACCTATTTT GAAGACCTAA AGCAGCGACT TGGTCACAGA 
CTGCAGCTCA CCGATTTGCT AATTAAACCA GTCCAGCGCA TAATGAAGTA CCAGCTGCTG 
CTAAAGGATC TACTCAAAGT TTCTAAAAAG GCAGGAGTGG ACACAGCAGA GCTGGAGAAA 
GCTGTTGAGG TGATGTGCGT GGTCCCAAAG CGTTGCAACG ACATGATGAA TGTTGGACGT 
CTTCAAGGCT TTGAAGGGAA AATTGTTGCT CAGGGCAGGC TGCTCCTCCA AGACACCTTC 
ATGGTTTCAG ACCAAGATGG GGGTCTACTG TCAAGGATGA AAGAAAGGAG AGTGTTTTTG 
TTTGAGCAGA TAGTTATATT CAGCGAACCT CTGGATAAAA AGAAGGGCTT CTCCACTCCT 
GGCTACCTCT TTAAAAACAG CATCAAGGTG AGCTGGCTGG GTCTGGAAGA AAACACAGAC 
GACCCCTGCA AGTTTACATT AACTTCAAGG TCATCTGCTG GGAGCATGGA GAGGTACACG 
CTTCACTCCG CCAGCCCAGG AGTGTGCCAA GTCTGGGTTC ACCAGGTCAG CCAGATCTTG 
GAAAATCAGC GGAACTTCCT CAACGCTTTG ACATCCCCCA TAGAGTACCA GAGGAACCAC 
GTGGGTGGAG GAGGGTCAAG CGGACCCCCT AGTAGTAGCA GCAGTAGTGC CGGAGGCCTC 
CCAGGAGGCA GCAGCACCAA CAATACCTCC AATTCGGGTG GAGGAGGAGG AGGTGGCGGA 
GGAGGAGGCT CAGGGGGTAA CTCCAATCTG TGCGGGCCTC GCTCTCGGCC CTCACGCATC 
CCGCAGCCTT CCTCGCGCCT CCCCCAGCCT GTTCACCACC ACCACCCCCC AGGCCCCGAG 
GGGCCTGACA GATCAGCAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023820064.1
CDS	226..7389
Translation	MSAGEGLDEAAKDAADIAAFFKSDCSLPKCESKLDGPGQTSGFHRNEDLKAIDVLPILKEKVAFLSGGRDRRGGPILTFPARSNHDRIRPEDLRRLVAYLATIPSEEVARHGFTVIVDMRGSKWDSIKPLLKILQESFPTCIHVALIIKPDNFWQKQRTNFGSSKFEFETVMVSLEGLTKIVDPSQLTADFEGSLEYNHDDWIEVRLSFETFASDAARALSRLEELQEVLSQRDLPRDLEGARRLMEEHAALKKRATKASIEELDTQGRRLLQRLQSHSAGGGNSSESGYGNRGVGASGDSNDHHHGLQGYSDAHSLVAKVTALLDKLHGTRQNLQQLWHMRKLKLDQCFQLRLFEQDAEKMFDWIMHNKGLFLTSYTEIGGNHQHAVELQTQHNHFAMNCMNVYVNINRIMSVGNRLLESGHYASQQIQQISGQLEQEWKAFAAALDERSTLLEMSASFHQKADQYMSKVEPWCKACGEGELPTELQDLEDTIHHHQGLYEHITTAYSEVSQEGKSLLDKLQRPLTPGSADSLMSSANYSKAVHHVLDIIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKVLLDMSVAFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQATVNVIKEGEDLIQQLRDSAISSNKTPHNSSMAHIESVLQQLDEAQGQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNEELSQQMNDFDTEDLTLAEQRLQHHADKALTMNNLTFDVIHQGQELLQYVTEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAADQHRRHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQKEHEQFQHAIEKTHQSALQVQQKAEALLQANHYDMDMIRDCAEKVADHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSESDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMLSHVKAPETQVKNILNELLQRENRVLHFWTMRKRRLDQCQQYVVFERSAKQALEWIHDTGEFYLSTHTSTGSSIHHTQELLKEHEDFQITAKQTKERVKLLIQLADGFCDKGHAHALEIKKWVSSVDKRYRDFSIRMDKYRTCLETALGISSDSNKASKGLQLDIIPASAPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPHGIINKEHIIFGNMQDLYEFHHNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVNYCKNKPDSTQLIVEHAGAYFDEIQQRHRLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLDGFDGNIDSQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSNGHSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSLENKQDWIKHIREVIQERTVHLRGALKEPIHIPKATATKHKGRRDGEDLDSQGDASSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFVASNGGSNGELTVRRGQTVEVLERLHDKPDWCLVRTTDRSPAQEGIVPCSMLCIAHSRSSMEMEGLFNHKDTLSVSSNDALQPGSSQTLGPHSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHIKKLAHKHKKNREGTRKNMEVIPGSQKDSDDSAATPQDETLEERMRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKSASRLSARPSSSETPSAAELVSAIEELVKSKMSLEDRPSSLSVEQGDSSSPSLNPSDNSLLSSSSPTDEMDERKSGFLKRRHYVLLELVETERDYVRDLGSVVEGYMSRMKEEGVPDDMRGKDKIVFGNIYQIYDWHKDFFLGELEKCLEDPERLATLFVKQERRLHMYIVYCQNKPKSEHIVSEYIDTYFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDLLKVSKKAGVDTAELEKAVEVMCVVPKRCNDMMNVGRLQGFEGKIVAQGRLLLQDTFMVSDQDGGLLSRMKERRVFLFEQIVIFSEPLDKKKGFSTPGYLFKNSIKVSWLGLEENTDDPCKFTLTSRSSAGSMERYTLHSASPGVCQVWVHQVSQILENQRNFLNALTSPIEYQRNHVGGGGSSGPPSSSSSSAGGLPGGSSTNNTSNSGGGGGGGGGGGSGGNSNLCGPRSRPSRIPQPSSRLPQPVHHHHPPGPEGPDRSAE

Target ORF information:

RefSeq Version	XM_023964296.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes triple functional domain protein (LOC101164837), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023964296.1

ORF Insert Sequence:

ATGAGCGCAG GAGAGGGTCT GGATGAGGCT GCGAAGGACG CGGCAGACAT AGCGGCGTTT 
TTCAAATCCG ATTGCTCTCT TCCAAAGTGT GAATCCAAAC TGGACGGCCC CGGTCAAACA 
TCAGGGTTCC ATCGCAACGA AGATTTGAAA GCCATTGATG TGCTTCCCAT CCTAAAAGAG 
AAGGTCGCCT TTCTTTCAGG TGGCAGAGAC AGACGTGGGG GTCCAATTCT CACTTTTCCA 
GCACGGAGCA ACCATGACAG AATACGACCC GAGGACCTGC GTAGGCTTGT AGCATACTTG 
GCTACTATTC CAAGTGAGGA GGTGGCCAGA CATGGCTTCA CCGTCATCGT GGACATGCGT 
GGTTCTAAAT GGGACAGCAT CAAACCGTTG CTGAAAATCC TACAGGAGTC TTTTCCCACC 
TGCATCCACG TTGCCCTCAT CATCAAACCG GACAACTTCT GGCAAAAACA GAGAACCAAC 
TTTGGCAGTT CTAAGTTTGA ATTTGAAACG GTGATGGTCT CATTAGAGGG CTTAACCAAA 
ATAGTGGACC CATCGCAGCT GACAGCTGAC TTTGAGGGCA GCTTAGAGTA CAACCATGAC 
GACTGGATCG AGGTGCGGCT ATCATTCGAG ACATTTGCCA GTGATGCAGC GAGGGCTTTG 
TCACGCCTCG AGGAGCTCCA GGAGGTCCTG TCCCAGCGTG ATCTCCCGCG GGACCTGGAA 
GGAGCCCGCC GCCTCATGGA AGAGCACGCT GCGCTGAAGA AAAGGGCCAC AAAGGCGTCC 
ATAGAGGAGC TTGACACGCA GGGCCGACGG CTGCTCCAAA GACTGCAGTC TCATTCTGCA 
GGGGGTGGAA ACAGCAGTGA AAGCGGATAC GGCAACCGCG GCGTGGGGGC CAGTGGAGAT 
TCCAACGACC ATCACCACGG GCTCCAGGGT TACAGCGACG CACACAGTCT AGTGGCTAAA 
GTTACGGCTT TGTTGGATAA GCTGCATGGG ACGCGGCAGA ACTTGCAGCA GCTGTGGCAC 
ATGAGGAAGC TAAAACTCGA CCAGTGTTTC CAGCTGCGGC TGTTTGAGCA AGATGCTGAG 
AAGATGTTCG ACTGGATTAT GCACAACAAG GGGCTTTTTC TTACCAGCTA CACTGAGATT 
GGGGGGAACC ACCAGCATGC TGTTGAGCTG CAGACACAGC ACAATCATTT TGCTATGAAC 
TGCATGAATG TCTATGTGAA CATCAACCGA ATCATGTCGG TGGGAAACCG ACTCCTGGAG 
TCTGGTCACT ACGCCTCCCA GCAGATCCAG CAGATCTCCG GCCAGCTGGA GCAGGAATGG 
AAAGCCTTTG CTGCAGCCCT GGATGAACGC AGCACACTGC TGGAGATGTC TGCCAGCTTC 
CATCAGAAGG CAGATCAGTA CATGAGTAAA GTGGAGCCAT GGTGCAAGGC ATGTGGAGAG 
GGAGAGCTGC CCACCGAACT CCAGGACCTG GAGGATACAA TCCACCATCA CCAGGGACTC 
TACGAGCACA TCACCACGGC ATACTCAGAG GTAAGCCAGG AAGGCAAATC TTTACTGGAC 
AAGCTGCAGC GACCGTTGAC CCCAGGAAGT GCCGATTCGT TGATGTCCTC GGCCAACTAC 
TCCAAGGCTG TGCACCACGT CCTGGATATC ATCCACGAGG TTTTGCACCA TCAGAGACAA 
CTGGAAAACA TATGGCAGCA TCGCAAAGTC CGACTTCATC AGCGGCTGCA GCTTTGTGTG 
TTCCAGCAGG ACGTGCAGCA GGTTTTGGAT TGGATCGAAA ATCATGGCGA GGCTTTCCTA 
AGTAAGCACA CCGGCGTGGG GAAATCTCTG CACAGAGCCA GGGCTCTGCA GAAACGACAC 
GAGGACTTTG AAGAAGTTGC CCAGAACACC TACACTAATG CTGACAAGTT GCTGGAGGCA 
GCAGAGCAGC TGGCCCAAAC TGGAGAGTGT GACCCAGAGG AAATATACCA GGCCGCCCAC 
CAGCTAGAAG ACCGCATCCA AGATTTTGTG CGGCGTGTGG AGCAGCGTAA AGTCTTGCTG 
GACATGTCTG TTGCATTTCA CACGCATGTC AAAGAGCTGT GGACGTGGTT GGAGGAGCTG 
CAGAAGGAAC TGCTGGACGA TGTGTATGCG GAGTCCGTGG AGGCGGTTCA GGACTTGATC 
AAGCGCTTCG GCCAGCAGCA GCAGACCACG CTGCAGGCAA CCGTCAATGT TATCAAAGAG 
GGAGAGGACC TTATACAGCA GCTCAGAGAC TCGGCTATCT CCAGCAACAA GACCCCTCAC 
AACAGCTCCA TGGCCCACAT CGAGAGCGTG CTGCAGCAGC TGGATGAAGC CCAGGGCCAG 
ATGGAAGAGC TGTTCCAGGA AAGGAAGATC AAGCTGGAGC TGTTTCTCCA ACTACGCATA 
TTTGAGAGGG ATGCCATTGA TATCATCTCC GACCTGGAGT CATGGAACGA GGAACTGTCC 
CAGCAGATGA ACGACTTCGA TACAGAGGAC CTGACTCTGG CAGAGCAGAG GCTGCAGCAT 
CACGCTGACA AAGCGCTCAC CATGAATAAC CTGACCTTTG ACGTCATTCA CCAGGGGCAG 
GAGCTGCTTC AGTACGTCAC CGAGGTCCAG GCGTCCGGTG TGGAGCTGCT ATGCGACAGA 
GACGTGGACA TGGCCACACG GGTTCAGGAC CTGCTGGAGT TTCTTCATGA GAAGCAGCAG 
GAGCTGGACT TGGCTGCAGA CCAGCACAGG AGGCACTTGG AGCAGTGTGT CCAGTTAAGG 
CACCTGCAAG CTGAAGTTAA ACAGGTGCTG GGTTGGATCC GCAATGGGGA GTCGATGTTG 
AATGCAGGTC TAATAACGGC TAGCTCCTTG CAAGAAGCTG AGCAGCTGCA GAAGGAGCAC 
GAACAGTTCC AGCATGCAAT AGAGAAAACA CACCAAAGTG CTCTGCAGGT GCAGCAGAAG 
GCAGAAGCTC TTCTCCAGGC CAACCACTAC GACATGGACA TGATCAGAGA CTGTGCTGAA 
AAGGTGGCCG ACCACTGGCA GCAGCTGATG CTGAAGATGG AGGACAGACT TAAACTGGTC 
AATGCTTCTG TGGCCTTCTA TAAAACATCA GAACAGGTGT GCAGTGTGTT GGAAAGTCTG 
GAGCAGGAGT ACAAGCGGGA GGAGGACTGG TGTGGTGGGG CTGATAAACT GGGCCCAAAC 
AGTGAGTCAG ATCACGTCAC ACCTATGATC AGCAAACATC TGGAGCAGAA AGAAGCTTTT 
CTTAAGGCCT GCACATTAGC CAGACGCAAT GCTGATGTTT TCCTGAAGTA TCTGCACAGA 
AACAGCGTCA ACATGCCGGG CATGTTATCT CATGTCAAAG CACCAGAGAC TCAGGTCAAG 
AACATTTTGA ATGAGTTGCT TCAGAGAGAA AACAGAGTCC TCCACTTCTG GACGATGAGG 
AAGCGGAGGT TGGACCAGTG CCAGCAATAT GTAGTGTTTG AAAGAAGTGC CAAACAGGCC 
TTGGAATGGA TCCACGACAC CGGCGAGTTT TACCTGTCCA CTCATACATC CACTGGCTCC 
AGCATTCATC ACACACAGGA GCTACTGAAA GAGCACGAAG ACTTCCAGAT CACAGCAAAG 
CAAACCAAAG AGCGGGTAAA GCTGTTGATC CAGCTGGCGG ATGGGTTTTG TGACAAGGGC 
CACGCCCATG CCCTGGAAAT AAAGAAATGG GTTTCCTCGG TGGACAAGCG CTACAGAGAC 
TTCTCTATCC GTATGGACAA ATACCGAACC TGCCTGGAAA CGGCGCTGGG CATCTCGTCT 
GACTCAAACA AGGCTAGTAA AGGACTGCAG TTAGACATAA TCCCAGCTAG TGCTCCAGGG 
TCAGAGGTCA AGTTGAGGGA TGCCGCTCAT GAACTGAACG AGGAGAAGCG GAAATCTGCA 
CGACGAAAAG AATTTATAAT GGCTGAGCTG ATTCAAACAG AGAAAGCATA CGTGAGAGAT 
CTGCGAGAGT GCATGGATAC GTACCTGTGG GAAATGACCA GTGGTGTGGA GGAGATTCCC 
CATGGCATCA TCAACAAGGA ACACATCATC TTTGGGAACA TGCAGGACCT CTATGAGTTT 
CATCACAACA TTTTCCTCAA AGAGCTGGAG AAGTATGAGC AGCTTCCAGA GGATGTTGGC 
CATTGCTTTG TGACCTGGGC TGATAAGTTC CAGATGTATG TGAACTACTG TAAGAACAAG 
CCAGACTCAA CTCAACTCAT TGTTGAGCAT GCGGGAGCCT ACTTCGATGA AATTCAGCAA 
AGACATCGTC TTGCCAATTC CATCTCGTCC TACCTGATCA AGCCGGTTCA GCGAATCACC 
AAATACCAAC TGCTTCTCAA GGAGTTGCTA ACATGCTGTG AGGAGGGAAA AGGTGAAATA 
AAAGATGGTC TGGAAGTGAT GCTCAGTGTT CCCAAGAGAG CCAATGATGC CATGCATCTC 
TCAATGCTGG ACGGTTTCGA TGGGAACATT GACTCCCAAG GAGAGTTGAT CCTCCAAGAG 
TCCTTCCAGG TGTGGGACCC CAAGACTCTG ATTCGGAAAG GTCGTGAAAG GCACCTCTTC 
CTCTTTGAGA TGTCTCTCGT CTTCAGCAAA GAAGTAAAAG ATTCCAACGG ACACAGCAAA 
TACCTCTACA AGAGCAAGCT CTTTACCTCT GAATTGGGTG TGACAGAGCA TGTGGAGGGA 
GATCCTTGTA AGTTTGCCCT GTGGGTGGGC AGGACCCCAA CCTCAGACAA CAAGATTGTT 
CTTAAGGCCT CAAGTCTAGA GAATAAGCAG GACTGGATCA AACACATCAG AGAGGTCATC 
CAAGAGCGAA CCGTCCACCT GCGCGGAGCT CTGAAGGAGC CCATTCACAT CCCAAAAGCC 
ACAGCTACCA AACACAAGGG CAGACGGGAT GGAGAGGATC TGGACAGCCA AGGAGATGCC 
AGCAGCCAAC CAGACACCAT TTCCATCGCC TCTCGCACAT CTCAGAACAC GCTGGACAGT 
GACAAGCTCT CGGGCGGCTG TGAGCTGACG GTGGTAATCC ACGACTTTGT GGCCAGCAAT 
GGGGGTAGCA ACGGGGAACT AACAGTCCGG CGGGGCCAAA CTGTGGAGGT TCTGGAGCGG 
CTGCACGACA AGCCGGACTG GTGCCTGGTC CGAACCACAG ATCGGTCCCC TGCCCAGGAA 
GGCATAGTGC CCTGCTCCAT GCTCTGCATC GCCCACTCCC GCTCTTCCAT GGAGATGGAG 
GGGCTGTTCA ACCACAAAGA CACCTTGTCT GTATCGAGTA ATGACGCCCT GCAGCCAGGC 
TCCAGCCAAA CTTTGGGCCC CCACAGCTCA CCCGGACCCA AACGTCCCGG GAACACCTTA 
AGGAAATGGT TGACGAGCCC CGTACGCCGA CTCAGCTCGG GCAAAGCCGA TGGTCACATC 
AAAAAGCTGG CCCACAAGCA CAAAAAGAAC CGCGAAGGCA CCAGGAAGAA CATGGAAGTG 
ATCCCGGGGT CGCAGAAGGA CTCCGACGAC AGCGCCGCCA CTCCACAGGA CGAGACTTTG 
GAGGAACGCA TGCGCAACGA AGGGCTAAGC AGCGGCACCC TCTCAAAGTC TTCCTCATCG 
GGGATGCAGA GCTGCGGCGA GGAGGAGGGA GAGGAGGGTG CGGACGCTGT TCCTCTGCCT 
CCCCCGATGG CCATCCAGCA GCACAGCCTG CTGCAGCCTG ACTCCCAGGA TGACAAGTCT 
GCGTCTCGTC TGTCCGCGCG TCCTAGTAGT TCAGAGACAC CCAGTGCTGC AGAATTGGTC 
AGTGCCATCG AGGAGTTGGT GAAGAGCAAG ATGTCGCTGG AGGACCGGCC CAGTTCTCTG 
TCCGTGGAGC AAGGCGACAG CAGCTCACCC TCTCTCAACC CCTCCGACAA CTCACTCCTC 
TCCTCGTCCT CACCCACGGA CGAGATGGAC GAGCGCAAAT CTGGCTTCCT CAAGCGAAGG 
CACTATGTGC TCCTTGAGTT AGTGGAGACT GAAAGGGACT ACGTCAGGGA CCTTGGATCT 
GTTGTGGAGG GCTACATGAG CAGAATGAAA GAGGAGGGAG TTCCAGATGA CATGAGAGGA 
AAAGACAAGA TTGTTTTTGG AAACATCTAC CAGATCTACG ACTGGCACAA AGATTTTTTC 
CTGGGAGAGC TGGAGAAGTG TTTGGAAGAC CCCGAGAGGC TGGCTACTTT ATTTGTCAAA 
CAGGAGCGGA GGCTGCACAT GTACATCGTG TACTGCCAGA ACAAGCCCAA ATCCGAGCAC 
ATTGTGTCCG AGTATATTGA CACCTATTTT GAAGACCTAA AGCAGCGACT TGGTCACAGA 
CTGCAGCTCA CCGATTTGCT AATTAAACCA GTCCAGCGCA TAATGAAGTA CCAGCTGCTG 
CTAAAGGATC TACTCAAAGT TTCTAAAAAG GCAGGAGTGG ACACAGCAGA GCTGGAGAAA 
GCTGTTGAGG TGATGTGCGT GGTCCCAAAG CGTTGCAACG ACATGATGAA TGTTGGACGT 
CTTCAAGGCT TTGAAGGGAA AATTGTTGCT CAGGGCAGGC TGCTCCTCCA AGACACCTTC 
ATGGTTTCAG ACCAAGATGG GGGTCTACTG TCAAGGATGA AAGAAAGGAG AGTGTTTTTG 
TTTGAGCAGA TAGTTATATT CAGCGAACCT CTGGATAAAA AGAAGGGCTT CTCCACTCCT 
GGCTACCTCT TTAAAAACAG CATCAAGGTG AGCTGGCTGG GTCTGGAAGA AAACACAGAC 
GACCCCTGCA AGTTTACATT AACTTCAAGG TCATCTGCTG GGAGCATGGA GAGGTACACG 
CTTCACTCCG CCAGCCCAGG AGTGTGCCAA GTCTGGGTTC ACCAGGTCAG CCAGATCTTG 
GAAAATCAGC GGAACTTCCT CAACGCTTTG ACATCCCCCA TAGAGTACCA GAGGAACCAC 
GTGGGTGGAG GAGGGTCAAG CGGACCCCCT AGTAGTAGCA GCAGTAGTGC CGGAGGCCTC 
CCAGGAGGCA GCAGCACCAA CAATACCTCC AATTCGGGTG GAGGAGGAGG AGGTGGCGGA 
GGAGGAGGCT CAGGGGGTAA CTCCAATCTG TGCGGGCCTC GCTCTCGGCC CTCACGCATC 
CCGCAGCCTT CCTCGCGCCT CCCCCAGCCT GTTCACCACC ACCACCCCCC AGGCCCCGAG 
GGGCCTGACA GATCAGCAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101164837 ( XM_020710033.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020565692.1 Oryzias latipes trio Rho guanine nucleotide exchange factor (trio), mRNA.

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CloneID	OJa72713
Clone ID Related Accession (Same CDS sequence)	XM_020710033.1 , XM_023964294.1
Accession Version	XM_020710033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	triple functional domain protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019874.1) and transcript sequence (GFIO01006648.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 440 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAGCGCAG GAGAGGGTCT GGATGAGGCT GCGAAGGACG CGGCAGACAT AGCGGCGTTT 
TTCAAATCCG ATTGCTCTCT TCCAAAGTGT GAATCCAAAC TGGACGGCCC CGGTCAAACA 
TCAGGGTTCC ATCGCAACGA AGATTTGAAA GCCATTGATG TGCTTCCCAT CCTAAAAGAG 
AAGGTCGCCT TTCTTTCAGG TGGCAGAGAC AGACGTGGGG GTCCAATTCT CACTTTTCCA 
GCACGGAGCA ACCATGACAG AATACGACCC GAGGACCTGC GTAGGCTTGT AGCATACTTG 
GCTACTATTC CAAGTGAGGA GGTGGCCAGA CATGGCTTCA CCGTCATCGT GGACATGCGT 
GGTTCTAAAT GGGACAGCAT CAAACCGTTG CTGAAAATCC TACAGGAGTC TTTTCCCACC 
TGCATCCACG TTGCCCTCAT CATCAAACCG GACAACTTCT GGCAAAAACA GAGAACCAAC 
TTTGGCAGTT CTAAGTTTGA ATTTGAAACG GTGATGGTCT CATTAGAGGG CTTAACCAAA 
ATAGTGGACC CATCGCAGCT GACAGCTGAC TTTGAGGGCA GCTTAGAGTA CAACCATGAC 
GACTGGATCG AGGTGCGGCT ATCATTCGAG ACATTTGCCA GTGATGCAGC GAGGGCTTTG 
TCACGCCTCG AGGAGCTCCA GGAGGTCCTG TCCCAGCGTG ATCTCCCGCG GGACCTGGAA 
GGAGCCCGCC GCCTCATGGA AGAGCACGCT GCGCTGAAGA AAAGGGCCAC AAAGGCGTCC 
ATAGAGGAGC TTGACACGCA GGGCCGACGG CTGCTCCAAA GACTGCAGTC TCATTCTGCA 
GGGGGTGGAA ACAGCAGTGA AAGCGGATAC GGCAACCGCG GCGTGGGGGC CAGTGGAGAT 
TCCAACGACC ATCACCACGG GCTCCAGGGT TACAGCGACG CACACAGTCT AGTGGCTAAA 
GTTACGGCTT TGTTGGATAA GCTGCATGGG ACGCGGCAGA ACTTGCAGCA GCTGTGGCAC 
ATGAGGAAGC TAAAACTCGA CCAGTGTTTC CAGCTGCGGC TGTTTGAGCA AGATGCTGAG 
AAGATGTTCG ACTGGATTAT GCACAACAAG GGGCTTTTTC TTACCAGCTA CACTGAGATT 
GGGGGGAACC ACCAGCATGC TGTTGAGCTG CAGACACAGC ACAATCATTT TGCTATGAAC 
TGCATGAATG TCTATGTGAA CATCAACCGA ATCATGTCGG TGGGAAACCG ACTCCTGGAG 
TCTGGTCACT ACGCCTCCCA GCAGATCCAG CAGATCTCCG GCCAGCTGGA GCAGGAATGG 
AAAGCCTTTG CTGCAGCCCT GGATGAACGC AGCACACTGC TGGAGATGTC TGCCAGCTTC 
CATCAGAAGG CAGATCAGTA CATGAGTAAA GTGGAGCCAT GGTGCAAGGC ATGTGGAGAG 
GGAGAGCTGC CCACCGAACT CCAGGACCTG GAGGATACAA TCCACCATCA CCAGGGACTC 
TACGAGCACA TCACCACGGC ATACTCAGAG GTAAGCCAGG AAGGCAAATC TTTACTGGAC 
AAGCTGCAGC GACCGTTGAC CCCAGGAAGT GCCGATTCGT TGATGTCCTC GGCCAACTAC 
TCCAAGGCTG TGCACCACGT CCTGGATATC ATCCACGAGG TTTTGCACCA TCAGAGACAA 
CTGGAAAACA TATGGCAGCA TCGCAAAGTC CGACTTCATC AGCGGCTGCA GCTTTGTGTG 
TTCCAGCAGG ACGTGCAGCA GGTTTTGGAT TGGATCGAAA ATCATGGCGA GGCTTTCCTA 
AGTAAGCACA CCGGCGTGGG GAAATCTCTG CACAGAGCCA GGGCTCTGCA GAAACGACAC 
GAGGACTTTG AAGAAGTTGC CCAGAACACC TACACTAATG CTGACAAGTT GCTGGAGGCA 
GCAGAGCAGC TGGCCCAAAC TGGAGAGTGT GACCCAGAGG AAATATACCA GGCCGCCCAC 
CAGCTAGAAG ACCGCATCCA AGATTTTGTG CGGCGTGTGG AGCAGCGTAA AGTCTTGCTG 
GACATGTCTG TTGCATTTCA CACGCATGTC AAAGAGCTGT GGACGTGGTT GGAGGAGCTG 
CAGAAGGAAC TGCTGGACGA TGTGTATGCG GAGTCCGTGG AGGCGGTTCA GGACTTGATC 
AAGCGCTTCG GCCAGCAGCA GCAGACCACG CTGCAGGCAA CCGTCAATGT TATCAAAGAG 
GGAGAGGACC TTATACAGCA GCTCAGAGAC TCGGCTATCT CCAGCAACAA GACCCCTCAC 
AACAGCTCCA TGGCCCACAT CGAGAGCGTG CTGCAGCAGC TGGATGAAGC CCAGGGCCAG 
ATGGAAGAGC TGTTCCAGGA AAGGAAGATC AAGCTGGAGC TGTTTCTCCA ACTACGCATA 
TTTGAGAGGG ATGCCATTGA TATCATCTCC GACCTGGAGT CATGGAACGA GGAACTGTCC 
CAGCAGATGA ACGACTTCGA TACAGAGGAC CTGACTCTGG CAGAGCAGAG GCTGCAGCAT 
CACGCTGACA AAGCGCTCAC CATGAATAAC CTGACCTTTG ACGTCATTCA CCAGGGGCAG 
GAGCTGCTTC AGTACGTCAC CGAGGTCCAG GCGTCCGGTG TGGAGCTGCT ATGCGACAGA 
GACGTGGACA TGGCCACACG GGTTCAGGAC CTGCTGGAGT TTCTTCATGA GAAGCAGCAG 
GAGCTGGACT TGGCTGCAGA CCAGCACAGG AGGCACTTGG AGCAGTGTGT CCAGTTAAGG 
CACCTGCAAG CTGAAGTTAA ACAGGTGCTG GGTTGGATCC GCAATGGGGA GTCGATGTTG 
AATGCAGGTC TAATAACGGC TAGCTCCTTG CAAGAAGCTG AGCAGCTGCA GAAGGAGCAC 
GAACAGTTCC AGCATGCAAT AGAGAAAACA CACCAAAGTG CTCTGCAGGT GCAGCAGAAG 
GCAGAAGCTC TTCTCCAGGC CAACCACTAC GACATGGACA TGATCAGAGA CTGTGCTGAA 
AAGGTGGCCG ACCACTGGCA GCAGCTGATG CTGAAGATGG AGGACAGACT TAAACTGGTC 
AATGCTTCTG TGGCCTTCTA TAAAACATCA GAACAGGTGT GCAGTGTGTT GGAAAGTCTG 
GAGCAGGAGT ACAAGCGGGA GGAGGACTGG TGTGGTGGGG CTGATAAACT GGGCCCAAAC 
AGTGAGTCAG ATCACGTCAC ACCTATGATC AGCAAACATC TGGAGCAGAA AGAAGCTTTT 
CTTAAGGCCT GCACATTAGC CAGACGCAAT GCTGATGTTT TCCTGAAGTA TCTGCACAGA 
AACAGCGTCA ACATGCCGGG CATGTTATCT CATGTCAAAG CACCAGAGAC TCAGGTCAAG 
AACATTTTGA ATGAGTTGCT TCAGAGAGAA AACAGAGTCC TCCACTTCTG GACGATGAGG 
AAGCGGAGGT TGGACCAGTG CCAGCAATAT GTAGTGTTTG AAAGAAGTGC CAAACAGGCC 
TTGGAATGGA TCCACGACAC CGGCGAGTTT TACCTGTCCA CTCATACATC CACTGGCTCC 
AGCATTCATC ACACACAGGA GCTACTGAAA GAGCACGAAG ACTTCCAGAT CACAGCAAAG 
CAAACCAAAG AGCGGGTAAA GCTGTTGATC CAGCTGGCGG ATGGGTTTTG TGACAAGGGC 
CACGCCCATG CCCTGGAAAT AAAGAAATGG GTTTCCTCGG TGGACAAGCG CTACAGAGAC 
TTCTCTATCC GTATGGACAA ATACCGAACC TGCCTGGAAA CGGCGCTGGG CATCTCGTCT 
GACTCAAACA AGGCTAGTAA AGGACTGCAG TTAGACATAA TCCCAGCTAG TGCTCCAGGG 
TCAGAGGTCA AGTTGAGGGA TGCCGCTCAT GAACTGAACG AGGAGAAGCG GAAATCTGCA 
CGACGAAAAG AATTTATAAT GGCTGAGCTG ATTCAAACAG AGAAAGCATA CGTGAGAGAT 
CTGCGAGAGT GCATGGATAC GTACCTGTGG GAAATGACCA GTGGTGTGGA GGAGATTCCC 
CATGGCATCA TCAACAAGGA ACACATCATC TTTGGGAACA TGCAGGACCT CTATGAGTTT 
CATCACAACA TTTTCCTCAA AGAGCTGGAG AAGTATGAGC AGCTTCCAGA GGATGTTGGC 
CATTGCTTTG TGACCTGGGC TGATAAGTTC CAGATGTATG TGAACTACTG TAAGAACAAG 
CCAGACTCAA CTCAACTCAT TGTTGAGCAT GCGGGAGCCT ACTTCGATGA AATTCAGCAA 
AGACATCGTC TTGCCAATTC CATCTCGTCC TACCTGATCA AGCCGGTTCA GCGAATCACC 
AAATACCAAC TGCTTCTCAA GGAGTTGCTA ACATGCTGTG AGGAGGGAAA AGGTGAAATA 
AAAGATGGTC TGGAAGTGAT GCTCAGTGTT CCCAAGAGAG CCAATGATGC CATGCATCTC 
TCAATGCTGG ACGGTTTCGA TGGGAACATT GACTCCCAAG GAGAGTTGAT CCTCCAAGAG 
TCCTTCCAGG TGTGGGACCC CAAGACTCTG ATTCGGAAAG GTCGTGAAAG GCACCTCTTC 
CTCTTTGAGA TGTCTCTCGT CTTCAGCAAA GAAGTAAAAG ATTCCAACGG ACACAGCAAA 
TACCTCTACA AGAGCAAGCT CTTTACCTCT GAATTGGGTG TGACAGAGCA TGTGGAGGGA 
GATCCTTGTA AGTTTGCCCT GTGGGTGGGC AGGACCCCAA CCTCAGACAA CAAGATTGTT 
CTTAAGGCCT CAAGTCTAGA GAATAAGCAG GACTGGATCA AACACATCAG AGAGGTCATC 
CAAGAGCGAA CCGTCCACCT GCGCGGAGCT CTGAAGGAGC CCATTCACAT CCCAAAAGCC 
ACAGCTACCA AACACAAGGG CAGACGGGAT GGAGAGGATC TGGACAGCCA AGGAGATGCC 
AGCAGCCAAC CAGACACCAT TTCCATCGCC TCTCGCACAT CTCAGAACAC GCTGGACAGT 
GACAAGCTCT CGGGCGGCTG TGAGCTGACG GTGGTAATCC ACGACTTTGT GGCCAGCAAT 
GGGGGTAGCA ACGGGGAACT AACAGTCCGG CGGGGCCAAA CTGTGGAGGT TCTGGAGCGG 
CTGCACGACA AGCCGGACTG GTGCCTGGTC CGAACCACAG ATCGGTCCCC TGCCCAGGAA 
GGCATAGTGC CCTGCTCCAT GCTCTGCATC GCCCACTCCC GCTCTTCCAT GGAGATGGAG 
GGGCTGTTCA ACCACAAAGA CACCTTGTCT GTATCGAGTA ATGACGCCCT GCAGCCAGGC 
TCCAGCCAAA CTTTGGGCCC CCACAGCTCA CCCGGACCCA AACGTCCCGG GAACACCTTA 
AGGAAATGGT TGACGAGCCC CGTACGCCGA CTCAGCTCGG GCAAAGCCGA TGGTCACATC 
AAAAAGCTGG CCCACAAGCA CAAAAAGAAC CGCGAAGGCA CCAGGAAGAA CATGGAAGTG 
ATCCCGGGGT CGCAGAAGGA CTCCGACGAC AGCGCCGCCA CTCCACAGGA CGAGACTTTG 
GAGGAACGCA TGCGCAACGA AGGGCTAAGC AGCGGCACCC TCTCAAAGTC TTCCTCATCG 
GGGATGCAGA GCTGCGGCGA GGAGGAGGGA GAGGAGGGTG CGGACGCTGT TCCTCTGCCT 
CCCCCGATGG CCATCCAGCA GCACAGCCTG CTGCAGCCTG ACTCCCAGGA TGACAAGTCT 
GCGTCTCGTC TGTCCGCGCG TCCTAGTAGT TCAGAGACAC CCAGTGCTGC AGAATTGGTC 
AGTGCCATCG AGGAGTTGGT GAAGAGCAAG ATGTCGCTGG AGGACCGGCC CAGTTCTCTG 
TCCGTGGAGC AAGGCGACAG CAGCTCACCC TCTCTCAACC CCTCCGACAA CTCACTCCTC 
TCCTCGTCCT CACCCACGGA CGAGATGGAC GAGCGCAAAT CTGGCTTCCT CAAGCGAAGG 
CACTATGTGC TCCTTGAGTT AGTGGAGACT GAAAGGGACT ACGTCAGGGA CCTTGGATCT 
GTTGTGGAGG GCTACATGAG CAGAATGAAA GAGGAGGGAG TTCCAGATGA CATGAGAGGA 
AAAGACAAGA TTGTTTTTGG AAACATCTAC CAGATCTACG ACTGGCACAA AGATTTTTTC 
CTGGGAGAGC TGGAGAAGTG TTTGGAAGAC CCCGAGAGGC TGGCTACTTT ATTTGTCAAA 
CAGGAGCGGA GGCTGCACAT GTACATCGTG TACTGCCAGA ACAAGCCCAA ATCCGAGCAC 
ATTGTGTCCG AGTATATTGA CACCTATTTT GAAGACCTAA AGCAGCGACT TGGTCACAGA 
CTGCAGCTCA CCGATTTGCT AATTAAACCA GTCCAGCGCA TAATGAAGTA CCAGCTGCTG 
CTAAAGGATC TACTCAAAGT TTCTAAAAAG GCAGGAGTGG ACACAGCAGA GCTGGAGAAA 
GCTGTTGAGG TGATGTGCGT GGTCCCAAAG CGTTGCAACG ACATGATGAA TGTTGGACGT 
CTTCAAGGCT TTGAAGGGAA AATTGTTGCT CAGGGCAGGC TGCTCCTCCA AGACACCTTC 
ATGGTTTCAG ACCAAGATGG GGGTCTACTG TCAAGGATGA AAGAAAGGAG AGTGTTTTTG 
TTTGAGCAGA TAGTTATATT CAGCGAACCT CTGGATAAAA AGAAGGGCTT CTCCACTCCT 
GGCTACCTCT TTAAAAACAG CATCAAGGTG AGCTGGCTGG GTCTGGAAGA AAACACAGAC 
GACCCCTGCA AGTTTACATT AACTTCAAGG TCATCTGCTG GGAGCATGGA GAGGTACACG 
CTTCACTCCG CCAGCCCAGG AGTGTGCCAA GTCTGGGTTC ACCAGGTCAG CCAGATCTTG 
GAAAATCAGC GGAACTTCCT CAACGCTTTG ACATCCCCCA TAGAGTACCA GAGGAACCAC 
GTGGGTGGAG GAGGGTCAAG CGGACCCCCT AGTAGTAGCA GCAGTAGTGC CGGAGGCCTC 
CCAGGAGGCA GCAGCACCAA CAATACCTCC AATTCGGGTG GAGGAGGAGG AGGTGGCGGA 
GGAGGAGGCT CAGGGGGTAA CTCCAATCTG TGCGGGCCTC GCTCTCGGCC CTCACGCATC 
CCGCAGCCTT CCTCGCGCCT CCCCCAGCCT GTTCACCACC ACCACCCCCC AGGCCCCGAG 
GGGCCTGACA GATCAGCAGG TATGTGGTCT CCCTGCCACC CGCAGCCCCC CGATTCCTTT 
CCCTCTAACT GCTTTTCTGA CAGCGCAACA AACGGAGAGC TGCCTGCCTC CGAGGTCCCT 
AAGATGAGGA TGGACAGTCC TCACGGAAGC AGCTGTAGTA ACAGTAACAG GCCGTTCAGT 
AATGCGGACA GCAGTGTCAG AAAGGGAGGA TGTGAGGAGA CGCAGAGGCC TCAGACCGCT 
GCGAAACCAC AGATGACAGT GGTTCCTTTG AATTTTCCTC TAAAAAGCCC CCGAGTCGGC 
ACCGTTTCCC CCCTAATGTC ATCTCCAGCT GCTGGAGCAG CAAAGGAACT GATCCTCCCT 
TCAAGCCCAG TGCATAAAGG AAACCCTTTC TGGGCTGTAG TCCCCCCTCT GCCTCCTTCG 
CCCGCCAGCA GGCCCGGTTC CTGCTCCTTC CCCAGTGACA ATAGTGGTGG AGGGGACTCT 
TTGGGCAGGG GAAGTCACGG GACGCCATCC CATCACCGCC ACTCCAGCCA TAATAAGGAT 
GTAGACCGCA TGAGCACCTG CTCCTCCACC AGCGAGCAGT CTGTTCACTC CACCCAGAGC 
AACGGGAGTG AAAGCAGTAG CAGCAGCAGC GTGTCCACCA TGTTGGTGAC CCAGGACTAT 
GTGGCAGTGA AGGAGGATGA GATCAGTGTG GCGCAGGGCG AGGTGGTCCA GATGCTTGCC 
AGCAACCAGC AGAACATGTT CCTGGTATAC CGGGCTGCCA ACGAGCACAG CCCTGCGGCT 
GAGGGCTGGA TCCCTGGCTA CGTTTTAGGC CACACCTCAA CCTCCGTCAC ACCCGAGCTT 
CCTGAAGGCA CCATCAAAAA ATCATTATCA TGGCACACAG CCCTCCGCAT CCGCCGAAAG 
TCTGAGAAAA GAGACAAGGA GGGGAAAAAA GTGGAGAATG GCTACCGCAA GTCTCAGGAC 
AGCCTGGCGA ACAAAGTCTC TGTCAAGCTT CTCAATCCCA ACTTCATTTA TGAGGCTCCA 
CCAGAGTTCC TCGTCCCTGT GAGCGATGCA TTATGTGAGA GCGGCGACAA CGTCATCCTG 
AGGTGTAAAG TATGTGGCCG TCCCCGAGCC ACCGTCACCT GGCACGGGCC GGACAATGAC 
ACCCTGAGCA GCGACGGACA CCACAGCATC ACATACAGTG AGACAGGTGA GGCATCTCTG 
CGCATCCTGG GAGCGTCTCC AGAGGATAGT GGGATGTACA CCTGTGTTGC CACGAACATC 
GCAGGCACCA GCTCCTCTTC TGCCAGTCTC ACAGTTTCAG GATCCCCCGA TGATGAAAGC 
GAAATCGTCT GGAAAAGCAG CTTTGAGTCC TATTTTACAG AGATAACTGA ACTGGGAAGG 
GGCCGCTTCT CAGTGACTAA GCGTTGTGAC CAGAGAGGAA GCAAACGAAC TGTAGCAGCA 
AAACACGTCA ACAAGAAGCT GCTGCGTCGT GAGAGGGCGC TGAAGGAGGT CCAACTCCTC 
CAGACTCTGG ACCATCCTAA CCTGGTCAAA CTGCTGGACA CATATGAGAC AGCCAACAGC 
TACGTGCTCA TCCTGGAGAT GGCAGACCAG GGCCGATTCC TGGATTACAT TGTGAGCTGG 
GGGAACCTGA CTGAAGAGAA GGTGGCTTTA TACCTTAGAG ACATTCTTGA GGCTCTTCAC 
TACCTGCACA GCTGGAGGAT AGCACACCTC GATCTCAAAC CTGAGAACAT TGTGGTGGAG 
CATGCATCCT CCCAGCCTGT AATCAAGCTG ACAGACTTTG GTGATGCCGT GCAGCTGAAC 
TCTGGTTGCT CCTATGTGCA TCCTCTGCTG GGAAGCCCCG AGTTCTCTGC TCCTGAGCTG 
GTTCTGGGTC AGCCCGCTTC CCTGATGTCT GACCTCTGGA GTTTAGGTGT GGTGACCTAC 
GTTGTCCTGA GTGGCGCCTC TCCCTTCCTG GACGAAAGCC TGGAAGAGAC GTGCCTGAAC 
ATCTGCCGCC TGGACTTCAG CTTCCCCGAA GATTACTTCC AGGGTGTGAG CCCAGCTGCC 
AGGGACTTTG TGTGCCGGCT GCTCCAAAGC GAGCCGGAAC GACGCCCCTC TGCGGCGTCC 
TGCCTCCAGG ATCCATGGCT GCAGCCCCGA GACGGCGGTC GCGACTCATC AAACCACACG 
CAGCCACACG CATTGCCACC ATCGCCGACC TTTCACAGCG TGCACCTGGA CACCTCCCGG 
CTTATCTCCT TCATTGAGAG ACGGAAGCAT CAGAACGACA TTCGGCCCGT CGGCGCCATC 
AAGGTCTTCC TTCACAGCCG CCTTCTCAAC CACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020565692.1
CDS	223..9681
Translation	MSAGEGLDEAAKDAADIAAFFKSDCSLPKCESKLDGPGQTSGFHRNEDLKAIDVLPILKEKVAFLSGGRDRRGGPILTFPARSNHDRIRPEDLRRLVAYLATIPSEEVARHGFTVIVDMRGSKWDSIKPLLKILQESFPTCIHVALIIKPDNFWQKQRTNFGSSKFEFETVMVSLEGLTKIVDPSQLTADFEGSLEYNHDDWIEVRLSFETFASDAARALSRLEELQEVLSQRDLPRDLEGARRLMEEHAALKKRATKASIEELDTQGRRLLQRLQSHSAGGGNSSESGYGNRGVGASGDSNDHHHGLQGYSDAHSLVAKVTALLDKLHGTRQNLQQLWHMRKLKLDQCFQLRLFEQDAEKMFDWIMHNKGLFLTSYTEIGGNHQHAVELQTQHNHFAMNCMNVYVNINRIMSVGNRLLESGHYASQQIQQISGQLEQEWKAFAAALDERSTLLEMSASFHQKADQYMSKVEPWCKACGEGELPTELQDLEDTIHHHQGLYEHITTAYSEVSQEGKSLLDKLQRPLTPGSADSLMSSANYSKAVHHVLDIIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKVLLDMSVAFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQATVNVIKEGEDLIQQLRDSAISSNKTPHNSSMAHIESVLQQLDEAQGQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNEELSQQMNDFDTEDLTLAEQRLQHHADKALTMNNLTFDVIHQGQELLQYVTEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAADQHRRHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQKEHEQFQHAIEKTHQSALQVQQKAEALLQANHYDMDMIRDCAEKVADHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSESDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMLSHVKAPETQVKNILNELLQRENRVLHFWTMRKRRLDQCQQYVVFERSAKQALEWIHDTGEFYLSTHTSTGSSIHHTQELLKEHEDFQITAKQTKERVKLLIQLADGFCDKGHAHALEIKKWVSSVDKRYRDFSIRMDKYRTCLETALGISSDSNKASKGLQLDIIPASAPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPHGIINKEHIIFGNMQDLYEFHHNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVNYCKNKPDSTQLIVEHAGAYFDEIQQRHRLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLDGFDGNIDSQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSNGHSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSLENKQDWIKHIREVIQERTVHLRGALKEPIHIPKATATKHKGRRDGEDLDSQGDASSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFVASNGGSNGELTVRRGQTVEVLERLHDKPDWCLVRTTDRSPAQEGIVPCSMLCIAHSRSSMEMEGLFNHKDTLSVSSNDALQPGSSQTLGPHSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHIKKLAHKHKKNREGTRKNMEVIPGSQKDSDDSAATPQDETLEERMRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKSASRLSARPSSSETPSAAELVSAIEELVKSKMSLEDRPSSLSVEQGDSSSPSLNPSDNSLLSSSSPTDEMDERKSGFLKRRHYVLLELVETERDYVRDLGSVVEGYMSRMKEEGVPDDMRGKDKIVFGNIYQIYDWHKDFFLGELEKCLEDPERLATLFVKQERRLHMYIVYCQNKPKSEHIVSEYIDTYFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDLLKVSKKAGVDTAELEKAVEVMCVVPKRCNDMMNVGRLQGFEGKIVAQGRLLLQDTFMVSDQDGGLLSRMKERRVFLFEQIVIFSEPLDKKKGFSTPGYLFKNSIKVSWLGLEENTDDPCKFTLTSRSSAGSMERYTLHSASPGVCQVWVHQVSQILENQRNFLNALTSPIEYQRNHVGGGGSSGPPSSSSSSAGGLPGGSSTNNTSNSGGGGGGGGGGGSGGNSNLCGPRSRPSRIPQPSSRLPQPVHHHHPPGPEGPDRSAGMWSPCHPQPPDSFPSNCFSDSATNGELPASEVPKMRMDSPHGSSCSNSNRPFSNADSSVRKGGCEETQRPQTAAKPQMTVVPLNFPLKSPRVGTVSPLMSSPAAGAAKELILPSSPVHKGNPFWAVVPPLPPSPASRPGSCSFPSDNSGGGDSLGRGSHGTPSHHRHSSHNKDVDRMSTCSSTSEQSVHSTQSNGSESSSSSSVSTMLVTQDYVAVKEDEISVAQGEVVQMLASNQQNMFLVYRAANEHSPAAEGWIPGYVLGHTSTSVTPELPEGTIKKSLSWHTALRIRRKSEKRDKEGKKVENGYRKSQDSLANKVSVKLLNPNFIYEAPPEFLVPVSDALCESGDNVILRCKVCGRPRATVTWHGPDNDTLSSDGHHSITYSETGEASLRILGASPEDSGMYTCVATNIAGTSSSSASLTVSGSPDDESEIVWKSSFESYFTEITELGRGRFSVTKRCDQRGSKRTVAAKHVNKKLLRRERALKEVQLLQTLDHPNLVKLLDTYETANSYVLILEMADQGRFLDYIVSWGNLTEEKVALYLRDILEALHYLHSWRIAHLDLKPENIVVEHASSQPVIKLTDFGDAVQLNSGCSYVHPLLGSPEFSAPELVLGQPASLMSDLWSLGVVTYVVLSGASPFLDESLEETCLNICRLDFSFPEDYFQGVSPAARDFVCRLLQSEPERRPSAASCLQDPWLQPRDGGRDSSNHTQPHALPPSPTFHSVHLDTSRLISFIERRKHQNDIRPVGAIKVFLHSRLLNHS

Target ORF information:

RefSeq Version	XM_020710033.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes trio Rho guanine nucleotide exchange factor (trio), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020710033.1

ORF Insert Sequence:

ATGAGCGCAG GAGAGGGTCT GGATGAGGCT GCGAAGGACG CGGCAGACAT AGCGGCGTTT 
TTCAAATCCG ATTGCTCTCT TCCAAAGTGT GAATCCAAAC TGGACGGCCC CGGTCAAACA 
TCAGGGTTCC ATCGCAACGA AGATTTGAAA GCCATTGATG TGCTTCCCAT CCTAAAAGAG 
AAGGTCGCCT TTCTTTCAGG TGGCAGAGAC AGACGTGGGG GTCCAATTCT CACTTTTCCA 
GCACGGAGCA ACCATGACAG AATACGACCC GAGGACCTGC GTAGGCTTGT AGCATACTTG 
GCTACTATTC CAAGTGAGGA GGTGGCCAGA CATGGCTTCA CCGTCATCGT GGACATGCGT 
GGTTCTAAAT GGGACAGCAT CAAACCGTTG CTGAAAATCC TACAGGAGTC TTTTCCCACC 
TGCATCCACG TTGCCCTCAT CATCAAACCG GACAACTTCT GGCAAAAACA GAGAACCAAC 
TTTGGCAGTT CTAAGTTTGA ATTTGAAACG GTGATGGTCT CATTAGAGGG CTTAACCAAA 
ATAGTGGACC CATCGCAGCT GACAGCTGAC TTTGAGGGCA GCTTAGAGTA CAACCATGAC 
GACTGGATCG AGGTGCGGCT ATCATTCGAG ACATTTGCCA GTGATGCAGC GAGGGCTTTG 
TCACGCCTCG AGGAGCTCCA GGAGGTCCTG TCCCAGCGTG ATCTCCCGCG GGACCTGGAA 
GGAGCCCGCC GCCTCATGGA AGAGCACGCT GCGCTGAAGA AAAGGGCCAC AAAGGCGTCC 
ATAGAGGAGC TTGACACGCA GGGCCGACGG CTGCTCCAAA GACTGCAGTC TCATTCTGCA 
GGGGGTGGAA ACAGCAGTGA AAGCGGATAC GGCAACCGCG GCGTGGGGGC CAGTGGAGAT 
TCCAACGACC ATCACCACGG GCTCCAGGGT TACAGCGACG CACACAGTCT AGTGGCTAAA 
GTTACGGCTT TGTTGGATAA GCTGCATGGG ACGCGGCAGA ACTTGCAGCA GCTGTGGCAC 
ATGAGGAAGC TAAAACTCGA CCAGTGTTTC CAGCTGCGGC TGTTTGAGCA AGATGCTGAG 
AAGATGTTCG ACTGGATTAT GCACAACAAG GGGCTTTTTC TTACCAGCTA CACTGAGATT 
GGGGGGAACC ACCAGCATGC TGTTGAGCTG CAGACACAGC ACAATCATTT TGCTATGAAC 
TGCATGAATG TCTATGTGAA CATCAACCGA ATCATGTCGG TGGGAAACCG ACTCCTGGAG 
TCTGGTCACT ACGCCTCCCA GCAGATCCAG CAGATCTCCG GCCAGCTGGA GCAGGAATGG 
AAAGCCTTTG CTGCAGCCCT GGATGAACGC AGCACACTGC TGGAGATGTC TGCCAGCTTC 
CATCAGAAGG CAGATCAGTA CATGAGTAAA GTGGAGCCAT GGTGCAAGGC ATGTGGAGAG 
GGAGAGCTGC CCACCGAACT CCAGGACCTG GAGGATACAA TCCACCATCA CCAGGGACTC 
TACGAGCACA TCACCACGGC ATACTCAGAG GTAAGCCAGG AAGGCAAATC TTTACTGGAC 
AAGCTGCAGC GACCGTTGAC CCCAGGAAGT GCCGATTCGT TGATGTCCTC GGCCAACTAC 
TCCAAGGCTG TGCACCACGT CCTGGATATC ATCCACGAGG TTTTGCACCA TCAGAGACAA 
CTGGAAAACA TATGGCAGCA TCGCAAAGTC CGACTTCATC AGCGGCTGCA GCTTTGTGTG 
TTCCAGCAGG ACGTGCAGCA GGTTTTGGAT TGGATCGAAA ATCATGGCGA GGCTTTCCTA 
AGTAAGCACA CCGGCGTGGG GAAATCTCTG CACAGAGCCA GGGCTCTGCA GAAACGACAC 
GAGGACTTTG AAGAAGTTGC CCAGAACACC TACACTAATG CTGACAAGTT GCTGGAGGCA 
GCAGAGCAGC TGGCCCAAAC TGGAGAGTGT GACCCAGAGG AAATATACCA GGCCGCCCAC 
CAGCTAGAAG ACCGCATCCA AGATTTTGTG CGGCGTGTGG AGCAGCGTAA AGTCTTGCTG 
GACATGTCTG TTGCATTTCA CACGCATGTC AAAGAGCTGT GGACGTGGTT GGAGGAGCTG 
CAGAAGGAAC TGCTGGACGA TGTGTATGCG GAGTCCGTGG AGGCGGTTCA GGACTTGATC 
AAGCGCTTCG GCCAGCAGCA GCAGACCACG CTGCAGGCAA CCGTCAATGT TATCAAAGAG 
GGAGAGGACC TTATACAGCA GCTCAGAGAC TCGGCTATCT CCAGCAACAA GACCCCTCAC 
AACAGCTCCA TGGCCCACAT CGAGAGCGTG CTGCAGCAGC TGGATGAAGC CCAGGGCCAG 
ATGGAAGAGC TGTTCCAGGA AAGGAAGATC AAGCTGGAGC TGTTTCTCCA ACTACGCATA 
TTTGAGAGGG ATGCCATTGA TATCATCTCC GACCTGGAGT CATGGAACGA GGAACTGTCC 
CAGCAGATGA ACGACTTCGA TACAGAGGAC CTGACTCTGG CAGAGCAGAG GCTGCAGCAT 
CACGCTGACA AAGCGCTCAC CATGAATAAC CTGACCTTTG ACGTCATTCA CCAGGGGCAG 
GAGCTGCTTC AGTACGTCAC CGAGGTCCAG GCGTCCGGTG TGGAGCTGCT ATGCGACAGA 
GACGTGGACA TGGCCACACG GGTTCAGGAC CTGCTGGAGT TTCTTCATGA GAAGCAGCAG 
GAGCTGGACT TGGCTGCAGA CCAGCACAGG AGGCACTTGG AGCAGTGTGT CCAGTTAAGG 
CACCTGCAAG CTGAAGTTAA ACAGGTGCTG GGTTGGATCC GCAATGGGGA GTCGATGTTG 
AATGCAGGTC TAATAACGGC TAGCTCCTTG CAAGAAGCTG AGCAGCTGCA GAAGGAGCAC 
GAACAGTTCC AGCATGCAAT AGAGAAAACA CACCAAAGTG CTCTGCAGGT GCAGCAGAAG 
GCAGAAGCTC TTCTCCAGGC CAACCACTAC GACATGGACA TGATCAGAGA CTGTGCTGAA 
AAGGTGGCCG ACCACTGGCA GCAGCTGATG CTGAAGATGG AGGACAGACT TAAACTGGTC 
AATGCTTCTG TGGCCTTCTA TAAAACATCA GAACAGGTGT GCAGTGTGTT GGAAAGTCTG 
GAGCAGGAGT ACAAGCGGGA GGAGGACTGG TGTGGTGGGG CTGATAAACT GGGCCCAAAC 
AGTGAGTCAG ATCACGTCAC ACCTATGATC AGCAAACATC TGGAGCAGAA AGAAGCTTTT 
CTTAAGGCCT GCACATTAGC CAGACGCAAT GCTGATGTTT TCCTGAAGTA TCTGCACAGA 
AACAGCGTCA ACATGCCGGG CATGTTATCT CATGTCAAAG CACCAGAGAC TCAGGTCAAG 
AACATTTTGA ATGAGTTGCT TCAGAGAGAA AACAGAGTCC TCCACTTCTG GACGATGAGG 
AAGCGGAGGT TGGACCAGTG CCAGCAATAT GTAGTGTTTG AAAGAAGTGC CAAACAGGCC 
TTGGAATGGA TCCACGACAC CGGCGAGTTT TACCTGTCCA CTCATACATC CACTGGCTCC 
AGCATTCATC ACACACAGGA GCTACTGAAA GAGCACGAAG ACTTCCAGAT CACAGCAAAG 
CAAACCAAAG AGCGGGTAAA GCTGTTGATC CAGCTGGCGG ATGGGTTTTG TGACAAGGGC 
CACGCCCATG CCCTGGAAAT AAAGAAATGG GTTTCCTCGG TGGACAAGCG CTACAGAGAC 
TTCTCTATCC GTATGGACAA ATACCGAACC TGCCTGGAAA CGGCGCTGGG CATCTCGTCT 
GACTCAAACA AGGCTAGTAA AGGACTGCAG TTAGACATAA TCCCAGCTAG TGCTCCAGGG 
TCAGAGGTCA AGTTGAGGGA TGCCGCTCAT GAACTGAACG AGGAGAAGCG GAAATCTGCA 
CGACGAAAAG AATTTATAAT GGCTGAGCTG ATTCAAACAG AGAAAGCATA CGTGAGAGAT 
CTGCGAGAGT GCATGGATAC GTACCTGTGG GAAATGACCA GTGGTGTGGA GGAGATTCCC 
CATGGCATCA TCAACAAGGA ACACATCATC TTTGGGAACA TGCAGGACCT CTATGAGTTT 
CATCACAACA TTTTCCTCAA AGAGCTGGAG AAGTATGAGC AGCTTCCAGA GGATGTTGGC 
CATTGCTTTG TGACCTGGGC TGATAAGTTC CAGATGTATG TGAACTACTG TAAGAACAAG 
CCAGACTCAA CTCAACTCAT TGTTGAGCAT GCGGGAGCCT ACTTCGATGA AATTCAGCAA 
AGACATCGTC TTGCCAATTC CATCTCGTCC TACCTGATCA AGCCGGTTCA GCGAATCACC 
AAATACCAAC TGCTTCTCAA GGAGTTGCTA ACATGCTGTG AGGAGGGAAA AGGTGAAATA 
AAAGATGGTC TGGAAGTGAT GCTCAGTGTT CCCAAGAGAG CCAATGATGC CATGCATCTC 
TCAATGCTGG ACGGTTTCGA TGGGAACATT GACTCCCAAG GAGAGTTGAT CCTCCAAGAG 
TCCTTCCAGG TGTGGGACCC CAAGACTCTG ATTCGGAAAG GTCGTGAAAG GCACCTCTTC 
CTCTTTGAGA TGTCTCTCGT CTTCAGCAAA GAAGTAAAAG ATTCCAACGG ACACAGCAAA 
TACCTCTACA AGAGCAAGCT CTTTACCTCT GAATTGGGTG TGACAGAGCA TGTGGAGGGA 
GATCCTTGTA AGTTTGCCCT GTGGGTGGGC AGGACCCCAA CCTCAGACAA CAAGATTGTT 
CTTAAGGCCT CAAGTCTAGA GAATAAGCAG GACTGGATCA AACACATCAG AGAGGTCATC 
CAAGAGCGAA CCGTCCACCT GCGCGGAGCT CTGAAGGAGC CCATTCACAT CCCAAAAGCC 
ACAGCTACCA AACACAAGGG CAGACGGGAT GGAGAGGATC TGGACAGCCA AGGAGATGCC 
AGCAGCCAAC CAGACACCAT TTCCATCGCC TCTCGCACAT CTCAGAACAC GCTGGACAGT 
GACAAGCTCT CGGGCGGCTG TGAGCTGACG GTGGTAATCC ACGACTTTGT GGCCAGCAAT 
GGGGGTAGCA ACGGGGAACT AACAGTCCGG CGGGGCCAAA CTGTGGAGGT TCTGGAGCGG 
CTGCACGACA AGCCGGACTG GTGCCTGGTC CGAACCACAG ATCGGTCCCC TGCCCAGGAA 
GGCATAGTGC CCTGCTCCAT GCTCTGCATC GCCCACTCCC GCTCTTCCAT GGAGATGGAG 
GGGCTGTTCA ACCACAAAGA CACCTTGTCT GTATCGAGTA ATGACGCCCT GCAGCCAGGC 
TCCAGCCAAA CTTTGGGCCC CCACAGCTCA CCCGGACCCA AACGTCCCGG GAACACCTTA 
AGGAAATGGT TGACGAGCCC CGTACGCCGA CTCAGCTCGG GCAAAGCCGA TGGTCACATC 
AAAAAGCTGG CCCACAAGCA CAAAAAGAAC CGCGAAGGCA CCAGGAAGAA CATGGAAGTG 
ATCCCGGGGT CGCAGAAGGA CTCCGACGAC AGCGCCGCCA CTCCACAGGA CGAGACTTTG 
GAGGAACGCA TGCGCAACGA AGGGCTAAGC AGCGGCACCC TCTCAAAGTC TTCCTCATCG 
GGGATGCAGA GCTGCGGCGA GGAGGAGGGA GAGGAGGGTG CGGACGCTGT TCCTCTGCCT 
CCCCCGATGG CCATCCAGCA GCACAGCCTG CTGCAGCCTG ACTCCCAGGA TGACAAGTCT 
GCGTCTCGTC TGTCCGCGCG TCCTAGTAGT TCAGAGACAC CCAGTGCTGC AGAATTGGTC 
AGTGCCATCG AGGAGTTGGT GAAGAGCAAG ATGTCGCTGG AGGACCGGCC CAGTTCTCTG 
TCCGTGGAGC AAGGCGACAG CAGCTCACCC TCTCTCAACC CCTCCGACAA CTCACTCCTC 
TCCTCGTCCT CACCCACGGA CGAGATGGAC GAGCGCAAAT CTGGCTTCCT CAAGCGAAGG 
CACTATGTGC TCCTTGAGTT AGTGGAGACT GAAAGGGACT ACGTCAGGGA CCTTGGATCT 
GTTGTGGAGG GCTACATGAG CAGAATGAAA GAGGAGGGAG TTCCAGATGA CATGAGAGGA 
AAAGACAAGA TTGTTTTTGG AAACATCTAC CAGATCTACG ACTGGCACAA AGATTTTTTC 
CTGGGAGAGC TGGAGAAGTG TTTGGAAGAC CCCGAGAGGC TGGCTACTTT ATTTGTCAAA 
CAGGAGCGGA GGCTGCACAT GTACATCGTG TACTGCCAGA ACAAGCCCAA ATCCGAGCAC 
ATTGTGTCCG AGTATATTGA CACCTATTTT GAAGACCTAA AGCAGCGACT TGGTCACAGA 
CTGCAGCTCA CCGATTTGCT AATTAAACCA GTCCAGCGCA TAATGAAGTA CCAGCTGCTG 
CTAAAGGATC TACTCAAAGT TTCTAAAAAG GCAGGAGTGG ACACAGCAGA GCTGGAGAAA 
GCTGTTGAGG TGATGTGCGT GGTCCCAAAG CGTTGCAACG ACATGATGAA TGTTGGACGT 
CTTCAAGGCT TTGAAGGGAA AATTGTTGCT CAGGGCAGGC TGCTCCTCCA AGACACCTTC 
ATGGTTTCAG ACCAAGATGG GGGTCTACTG TCAAGGATGA AAGAAAGGAG AGTGTTTTTG 
TTTGAGCAGA TAGTTATATT CAGCGAACCT CTGGATAAAA AGAAGGGCTT CTCCACTCCT 
GGCTACCTCT TTAAAAACAG CATCAAGGTG AGCTGGCTGG GTCTGGAAGA AAACACAGAC 
GACCCCTGCA AGTTTACATT AACTTCAAGG TCATCTGCTG GGAGCATGGA GAGGTACACG 
CTTCACTCCG CCAGCCCAGG AGTGTGCCAA GTCTGGGTTC ACCAGGTCAG CCAGATCTTG 
GAAAATCAGC GGAACTTCCT CAACGCTTTG ACATCCCCCA TAGAGTACCA GAGGAACCAC 
GTGGGTGGAG GAGGGTCAAG CGGACCCCCT AGTAGTAGCA GCAGTAGTGC CGGAGGCCTC 
CCAGGAGGCA GCAGCACCAA CAATACCTCC AATTCGGGTG GAGGAGGAGG AGGTGGCGGA 
GGAGGAGGCT CAGGGGGTAA CTCCAATCTG TGCGGGCCTC GCTCTCGGCC CTCACGCATC 
CCGCAGCCTT CCTCGCGCCT CCCCCAGCCT GTTCACCACC ACCACCCCCC AGGCCCCGAG 
GGGCCTGACA GATCAGCAGG TATGTGGTCT CCCTGCCACC CGCAGCCCCC CGATTCCTTT 
CCCTCTAACT GCTTTTCTGA CAGCGCAACA AACGGAGAGC TGCCTGCCTC CGAGGTCCCT 
AAGATGAGGA TGGACAGTCC TCACGGAAGC AGCTGTAGTA ACAGTAACAG GCCGTTCAGT 
AATGCGGACA GCAGTGTCAG AAAGGGAGGA TGTGAGGAGA CGCAGAGGCC TCAGACCGCT 
GCGAAACCAC AGATGACAGT GGTTCCTTTG AATTTTCCTC TAAAAAGCCC CCGAGTCGGC 
ACCGTTTCCC CCCTAATGTC ATCTCCAGCT GCTGGAGCAG CAAAGGAACT GATCCTCCCT 
TCAAGCCCAG TGCATAAAGG AAACCCTTTC TGGGCTGTAG TCCCCCCTCT GCCTCCTTCG 
CCCGCCAGCA GGCCCGGTTC CTGCTCCTTC CCCAGTGACA ATAGTGGTGG AGGGGACTCT 
TTGGGCAGGG GAAGTCACGG GACGCCATCC CATCACCGCC ACTCCAGCCA TAATAAGGAT 
GTAGACCGCA TGAGCACCTG CTCCTCCACC AGCGAGCAGT CTGTTCACTC CACCCAGAGC 
AACGGGAGTG AAAGCAGTAG CAGCAGCAGC GTGTCCACCA TGTTGGTGAC CCAGGACTAT 
GTGGCAGTGA AGGAGGATGA GATCAGTGTG GCGCAGGGCG AGGTGGTCCA GATGCTTGCC 
AGCAACCAGC AGAACATGTT CCTGGTATAC CGGGCTGCCA ACGAGCACAG CCCTGCGGCT 
GAGGGCTGGA TCCCTGGCTA CGTTTTAGGC CACACCTCAA CCTCCGTCAC ACCCGAGCTT 
CCTGAAGGCA CCATCAAAAA ATCATTATCA TGGCACACAG CCCTCCGCAT CCGCCGAAAG 
TCTGAGAAAA GAGACAAGGA GGGGAAAAAA GTGGAGAATG GCTACCGCAA GTCTCAGGAC 
AGCCTGGCGA ACAAAGTCTC TGTCAAGCTT CTCAATCCCA ACTTCATTTA TGAGGCTCCA 
CCAGAGTTCC TCGTCCCTGT GAGCGATGCA TTATGTGAGA GCGGCGACAA CGTCATCCTG 
AGGTGTAAAG TATGTGGCCG TCCCCGAGCC ACCGTCACCT GGCACGGGCC GGACAATGAC 
ACCCTGAGCA GCGACGGACA CCACAGCATC ACATACAGTG AGACAGGTGA GGCATCTCTG 
CGCATCCTGG GAGCGTCTCC AGAGGATAGT GGGATGTACA CCTGTGTTGC CACGAACATC 
GCAGGCACCA GCTCCTCTTC TGCCAGTCTC ACAGTTTCAG GATCCCCCGA TGATGAAAGC 
GAAATCGTCT GGAAAAGCAG CTTTGAGTCC TATTTTACAG AGATAACTGA ACTGGGAAGG 
GGCCGCTTCT CAGTGACTAA GCGTTGTGAC CAGAGAGGAA GCAAACGAAC TGTAGCAGCA 
AAACACGTCA ACAAGAAGCT GCTGCGTCGT GAGAGGGCGC TGAAGGAGGT CCAACTCCTC 
CAGACTCTGG ACCATCCTAA CCTGGTCAAA CTGCTGGACA CATATGAGAC AGCCAACAGC 
TACGTGCTCA TCCTGGAGAT GGCAGACCAG GGCCGATTCC TGGATTACAT TGTGAGCTGG 
GGGAACCTGA CTGAAGAGAA GGTGGCTTTA TACCTTAGAG ACATTCTTGA GGCTCTTCAC 
TACCTGCACA GCTGGAGGAT AGCACACCTC GATCTCAAAC CTGAGAACAT TGTGGTGGAG 
CATGCATCCT CCCAGCCTGT AATCAAGCTG ACAGACTTTG GTGATGCCGT GCAGCTGAAC 
TCTGGTTGCT CCTATGTGCA TCCTCTGCTG GGAAGCCCCG AGTTCTCTGC TCCTGAGCTG 
GTTCTGGGTC AGCCCGCTTC CCTGATGTCT GACCTCTGGA GTTTAGGTGT GGTGACCTAC 
GTTGTCCTGA GTGGCGCCTC TCCCTTCCTG GACGAAAGCC TGGAAGAGAC GTGCCTGAAC 
ATCTGCCGCC TGGACTTCAG CTTCCCCGAA GATTACTTCC AGGGTGTGAG CCCAGCTGCC 
AGGGACTTTG TGTGCCGGCT GCTCCAAAGC GAGCCGGAAC GACGCCCCTC TGCGGCGTCC 
TGCCTCCAGG ATCCATGGCT GCAGCCCCGA GACGGCGGTC GCGACTCATC AAACCACACG 
CAGCCACACG CATTGCCACC ATCGCCGACC TTTCACAGCG TGCACCTGGA CACCTCCCGG 
CTTATCTCCT TCATTGAGAG ACGGAAGCAT CAGAACGACA TTCGGCCCGT CGGCGCCATC 
AAGGTCTTCC TTCACAGCCG CCTTCTCAAC CACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101164837 ( XM_023964294.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023820062.1 Oryzias latipes triple functional domain protein (LOC101164837), transcript variant X1, mRNA.

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CloneID	OJa72713
Clone ID Related Accession (Same CDS sequence)	XM_020710033.1 , XM_023964294.1
Accession Version	XM_023964294.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	triple functional domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019874.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCAG GAGAGGGTCT GGATGAGGCT GCGAAGGACG CGGCAGACAT AGCGGCGTTT 
TTCAAATCCG ATTGCTCTCT TCCAAAGTGT GAATCCAAAC TGGACGGCCC CGGTCAAACA 
TCAGGGTTCC ATCGCAACGA AGATTTGAAA GCCATTGATG TGCTTCCCAT CCTAAAAGAG 
AAGGTCGCCT TTCTTTCAGG TGGCAGAGAC AGACGTGGGG GTCCAATTCT CACTTTTCCA 
GCACGGAGCA ACCATGACAG AATACGACCC GAGGACCTGC GTAGGCTTGT AGCATACTTG 
GCTACTATTC CAAGTGAGGA GGTGGCCAGA CATGGCTTCA CCGTCATCGT GGACATGCGT 
GGTTCTAAAT GGGACAGCAT CAAACCGTTG CTGAAAATCC TACAGGAGTC TTTTCCCACC 
TGCATCCACG TTGCCCTCAT CATCAAACCG GACAACTTCT GGCAAAAACA GAGAACCAAC 
TTTGGCAGTT CTAAGTTTGA ATTTGAAACG GTGATGGTCT CATTAGAGGG CTTAACCAAA 
ATAGTGGACC CATCGCAGCT GACAGCTGAC TTTGAGGGCA GCTTAGAGTA CAACCATGAC 
GACTGGATCG AGGTGCGGCT ATCATTCGAG ACATTTGCCA GTGATGCAGC GAGGGCTTTG 
TCACGCCTCG AGGAGCTCCA GGAGGTCCTG TCCCAGCGTG ATCTCCCGCG GGACCTGGAA 
GGAGCCCGCC GCCTCATGGA AGAGCACGCT GCGCTGAAGA AAAGGGCCAC AAAGGCGTCC 
ATAGAGGAGC TTGACACGCA GGGCCGACGG CTGCTCCAAA GACTGCAGTC TCATTCTGCA 
GGGGGTGGAA ACAGCAGTGA AAGCGGATAC GGCAACCGCG GCGTGGGGGC CAGTGGAGAT 
TCCAACGACC ATCACCACGG GCTCCAGGGT TACAGCGACG CACACAGTCT AGTGGCTAAA 
GTTACGGCTT TGTTGGATAA GCTGCATGGG ACGCGGCAGA ACTTGCAGCA GCTGTGGCAC 
ATGAGGAAGC TAAAACTCGA CCAGTGTTTC CAGCTGCGGC TGTTTGAGCA AGATGCTGAG 
AAGATGTTCG ACTGGATTAT GCACAACAAG GGGCTTTTTC TTACCAGCTA CACTGAGATT 
GGGGGGAACC ACCAGCATGC TGTTGAGCTG CAGACACAGC ACAATCATTT TGCTATGAAC 
TGCATGAATG TCTATGTGAA CATCAACCGA ATCATGTCGG TGGGAAACCG ACTCCTGGAG 
TCTGGTCACT ACGCCTCCCA GCAGATCCAG CAGATCTCCG GCCAGCTGGA GCAGGAATGG 
AAAGCCTTTG CTGCAGCCCT GGATGAACGC AGCACACTGC TGGAGATGTC TGCCAGCTTC 
CATCAGAAGG CAGATCAGTA CATGAGTAAA GTGGAGCCAT GGTGCAAGGC ATGTGGAGAG 
GGAGAGCTGC CCACCGAACT CCAGGACCTG GAGGATACAA TCCACCATCA CCAGGGACTC 
TACGAGCACA TCACCACGGC ATACTCAGAG GTAAGCCAGG AAGGCAAATC TTTACTGGAC 
AAGCTGCAGC GACCGTTGAC CCCAGGAAGT GCCGATTCGT TGATGTCCTC GGCCAACTAC 
TCCAAGGCTG TGCACCACGT CCTGGATATC ATCCACGAGG TTTTGCACCA TCAGAGACAA 
CTGGAAAACA TATGGCAGCA TCGCAAAGTC CGACTTCATC AGCGGCTGCA GCTTTGTGTG 
TTCCAGCAGG ACGTGCAGCA GGTTTTGGAT TGGATCGAAA ATCATGGCGA GGCTTTCCTA 
AGTAAGCACA CCGGCGTGGG GAAATCTCTG CACAGAGCCA GGGCTCTGCA GAAACGACAC 
GAGGACTTTG AAGAAGTTGC CCAGAACACC TACACTAATG CTGACAAGTT GCTGGAGGCA 
GCAGAGCAGC TGGCCCAAAC TGGAGAGTGT GACCCAGAGG AAATATACCA GGCCGCCCAC 
CAGCTAGAAG ACCGCATCCA AGATTTTGTG CGGCGTGTGG AGCAGCGTAA AGTCTTGCTG 
GACATGTCTG TTGCATTTCA CACGCATGTC AAAGAGCTGT GGACGTGGTT GGAGGAGCTG 
CAGAAGGAAC TGCTGGACGA TGTGTATGCG GAGTCCGTGG AGGCGGTTCA GGACTTGATC 
AAGCGCTTCG GCCAGCAGCA GCAGACCACG CTGCAGGCAA CCGTCAATGT TATCAAAGAG 
GGAGAGGACC TTATACAGCA GCTCAGAGAC TCGGCTATCT CCAGCAACAA GACCCCTCAC 
AACAGCTCCA TGGCCCACAT CGAGAGCGTG CTGCAGCAGC TGGATGAAGC CCAGGGCCAG 
ATGGAAGAGC TGTTCCAGGA AAGGAAGATC AAGCTGGAGC TGTTTCTCCA ACTACGCATA 
TTTGAGAGGG ATGCCATTGA TATCATCTCC GACCTGGAGT CATGGAACGA GGAACTGTCC 
CAGCAGATGA ACGACTTCGA TACAGAGGAC CTGACTCTGG CAGAGCAGAG GCTGCAGCAT 
CACGCTGACA AAGCGCTCAC CATGAATAAC CTGACCTTTG ACGTCATTCA CCAGGGGCAG 
GAGCTGCTTC AGTACGTCAC CGAGGTCCAG GCGTCCGGTG TGGAGCTGCT ATGCGACAGA 
GACGTGGACA TGGCCACACG GGTTCAGGAC CTGCTGGAGT TTCTTCATGA GAAGCAGCAG 
GAGCTGGACT TGGCTGCAGA CCAGCACAGG AGGCACTTGG AGCAGTGTGT CCAGTTAAGG 
CACCTGCAAG CTGAAGTTAA ACAGGTGCTG GGTTGGATCC GCAATGGGGA GTCGATGTTG 
AATGCAGGTC TAATAACGGC TAGCTCCTTG CAAGAAGCTG AGCAGCTGCA GAAGGAGCAC 
GAACAGTTCC AGCATGCAAT AGAGAAAACA CACCAAAGTG CTCTGCAGGT GCAGCAGAAG 
GCAGAAGCTC TTCTCCAGGC CAACCACTAC GACATGGACA TGATCAGAGA CTGTGCTGAA 
AAGGTGGCCG ACCACTGGCA GCAGCTGATG CTGAAGATGG AGGACAGACT TAAACTGGTC 
AATGCTTCTG TGGCCTTCTA TAAAACATCA GAACAGGTGT GCAGTGTGTT GGAAAGTCTG 
GAGCAGGAGT ACAAGCGGGA GGAGGACTGG TGTGGTGGGG CTGATAAACT GGGCCCAAAC 
AGTGAGTCAG ATCACGTCAC ACCTATGATC AGCAAACATC TGGAGCAGAA AGAAGCTTTT 
CTTAAGGCCT GCACATTAGC CAGACGCAAT GCTGATGTTT TCCTGAAGTA TCTGCACAGA 
AACAGCGTCA ACATGCCGGG CATGTTATCT CATGTCAAAG CACCAGAGAC TCAGGTCAAG 
AACATTTTGA ATGAGTTGCT TCAGAGAGAA AACAGAGTCC TCCACTTCTG GACGATGAGG 
AAGCGGAGGT TGGACCAGTG CCAGCAATAT GTAGTGTTTG AAAGAAGTGC CAAACAGGCC 
TTGGAATGGA TCCACGACAC CGGCGAGTTT TACCTGTCCA CTCATACATC CACTGGCTCC 
AGCATTCATC ACACACAGGA GCTACTGAAA GAGCACGAAG ACTTCCAGAT CACAGCAAAG 
CAAACCAAAG AGCGGGTAAA GCTGTTGATC CAGCTGGCGG ATGGGTTTTG TGACAAGGGC 
CACGCCCATG CCCTGGAAAT AAAGAAATGG GTTTCCTCGG TGGACAAGCG CTACAGAGAC 
TTCTCTATCC GTATGGACAA ATACCGAACC TGCCTGGAAA CGGCGCTGGG CATCTCGTCT 
GACTCAAACA AGGCTAGTAA AGGACTGCAG TTAGACATAA TCCCAGCTAG TGCTCCAGGG 
TCAGAGGTCA AGTTGAGGGA TGCCGCTCAT GAACTGAACG AGGAGAAGCG GAAATCTGCA 
CGACGAAAAG AATTTATAAT GGCTGAGCTG ATTCAAACAG AGAAAGCATA CGTGAGAGAT 
CTGCGAGAGT GCATGGATAC GTACCTGTGG GAAATGACCA GTGGTGTGGA GGAGATTCCC 
CATGGCATCA TCAACAAGGA ACACATCATC TTTGGGAACA TGCAGGACCT CTATGAGTTT 
CATCACAACA TTTTCCTCAA AGAGCTGGAG AAGTATGAGC AGCTTCCAGA GGATGTTGGC 
CATTGCTTTG TGACCTGGGC TGATAAGTTC CAGATGTATG TGAACTACTG TAAGAACAAG 
CCAGACTCAA CTCAACTCAT TGTTGAGCAT GCGGGAGCCT ACTTCGATGA AATTCAGCAA 
AGACATCGTC TTGCCAATTC CATCTCGTCC TACCTGATCA AGCCGGTTCA GCGAATCACC 
AAATACCAAC TGCTTCTCAA GGAGTTGCTA ACATGCTGTG AGGAGGGAAA AGGTGAAATA 
AAAGATGGTC TGGAAGTGAT GCTCAGTGTT CCCAAGAGAG CCAATGATGC CATGCATCTC 
TCAATGCTGG ACGGTTTCGA TGGGAACATT GACTCCCAAG GAGAGTTGAT CCTCCAAGAG 
TCCTTCCAGG TGTGGGACCC CAAGACTCTG ATTCGGAAAG GTCGTGAAAG GCACCTCTTC 
CTCTTTGAGA TGTCTCTCGT CTTCAGCAAA GAAGTAAAAG ATTCCAACGG ACACAGCAAA 
TACCTCTACA AGAGCAAGCT CTTTACCTCT GAATTGGGTG TGACAGAGCA TGTGGAGGGA 
GATCCTTGTA AGTTTGCCCT GTGGGTGGGC AGGACCCCAA CCTCAGACAA CAAGATTGTT 
CTTAAGGCCT CAAGTCTAGA GAATAAGCAG GACTGGATCA AACACATCAG AGAGGTCATC 
CAAGAGCGAA CCGTCCACCT GCGCGGAGCT CTGAAGGAGC CCATTCACAT CCCAAAAGCC 
ACAGCTACCA AACACAAGGG CAGACGGGAT GGAGAGGATC TGGACAGCCA AGGAGATGCC 
AGCAGCCAAC CAGACACCAT TTCCATCGCC TCTCGCACAT CTCAGAACAC GCTGGACAGT 
GACAAGCTCT CGGGCGGCTG TGAGCTGACG GTGGTAATCC ACGACTTTGT GGCCAGCAAT 
GGGGGTAGCA ACGGGGAACT AACAGTCCGG CGGGGCCAAA CTGTGGAGGT TCTGGAGCGG 
CTGCACGACA AGCCGGACTG GTGCCTGGTC CGAACCACAG ATCGGTCCCC TGCCCAGGAA 
GGCATAGTGC CCTGCTCCAT GCTCTGCATC GCCCACTCCC GCTCTTCCAT GGAGATGGAG 
GGGCTGTTCA ACCACAAAGA CACCTTGTCT GTATCGAGTA ATGACGCCCT GCAGCCAGGC 
TCCAGCCAAA CTTTGGGCCC CCACAGCTCA CCCGGACCCA AACGTCCCGG GAACACCTTA 
AGGAAATGGT TGACGAGCCC CGTACGCCGA CTCAGCTCGG GCAAAGCCGA TGGTCACATC 
AAAAAGCTGG CCCACAAGCA CAAAAAGAAC CGCGAAGGCA CCAGGAAGAA CATGGAAGTG 
ATCCCGGGGT CGCAGAAGGA CTCCGACGAC AGCGCCGCCA CTCCACAGGA CGAGACTTTG 
GAGGAACGCA TGCGCAACGA AGGGCTAAGC AGCGGCACCC TCTCAAAGTC TTCCTCATCG 
GGGATGCAGA GCTGCGGCGA GGAGGAGGGA GAGGAGGGTG CGGACGCTGT TCCTCTGCCT 
CCCCCGATGG CCATCCAGCA GCACAGCCTG CTGCAGCCTG ACTCCCAGGA TGACAAGTCT 
GCGTCTCGTC TGTCCGCGCG TCCTAGTAGT TCAGAGACAC CCAGTGCTGC AGAATTGGTC 
AGTGCCATCG AGGAGTTGGT GAAGAGCAAG ATGTCGCTGG AGGACCGGCC CAGTTCTCTG 
TCCGTGGAGC AAGGCGACAG CAGCTCACCC TCTCTCAACC CCTCCGACAA CTCACTCCTC 
TCCTCGTCCT CACCCACGGA CGAGATGGAC GAGCGCAAAT CTGGCTTCCT CAAGCGAAGG 
CACTATGTGC TCCTTGAGTT AGTGGAGACT GAAAGGGACT ACGTCAGGGA CCTTGGATCT 
GTTGTGGAGG GCTACATGAG CAGAATGAAA GAGGAGGGAG TTCCAGATGA CATGAGAGGA 
AAAGACAAGA TTGTTTTTGG AAACATCTAC CAGATCTACG ACTGGCACAA AGATTTTTTC 
CTGGGAGAGC TGGAGAAGTG TTTGGAAGAC CCCGAGAGGC TGGCTACTTT ATTTGTCAAA 
CAGGAGCGGA GGCTGCACAT GTACATCGTG TACTGCCAGA ACAAGCCCAA ATCCGAGCAC 
ATTGTGTCCG AGTATATTGA CACCTATTTT GAAGACCTAA AGCAGCGACT TGGTCACAGA 
CTGCAGCTCA CCGATTTGCT AATTAAACCA GTCCAGCGCA TAATGAAGTA CCAGCTGCTG 
CTAAAGGATC TACTCAAAGT TTCTAAAAAG GCAGGAGTGG ACACAGCAGA GCTGGAGAAA 
GCTGTTGAGG TGATGTGCGT GGTCCCAAAG CGTTGCAACG ACATGATGAA TGTTGGACGT 
CTTCAAGGCT TTGAAGGGAA AATTGTTGCT CAGGGCAGGC TGCTCCTCCA AGACACCTTC 
ATGGTTTCAG ACCAAGATGG GGGTCTACTG TCAAGGATGA AAGAAAGGAG AGTGTTTTTG 
TTTGAGCAGA TAGTTATATT CAGCGAACCT CTGGATAAAA AGAAGGGCTT CTCCACTCCT 
GGCTACCTCT TTAAAAACAG CATCAAGGTG AGCTGGCTGG GTCTGGAAGA AAACACAGAC 
GACCCCTGCA AGTTTACATT AACTTCAAGG TCATCTGCTG GGAGCATGGA GAGGTACACG 
CTTCACTCCG CCAGCCCAGG AGTGTGCCAA GTCTGGGTTC ACCAGGTCAG CCAGATCTTG 
GAAAATCAGC GGAACTTCCT CAACGCTTTG ACATCCCCCA TAGAGTACCA GAGGAACCAC 
GTGGGTGGAG GAGGGTCAAG CGGACCCCCT AGTAGTAGCA GCAGTAGTGC CGGAGGCCTC 
CCAGGAGGCA GCAGCACCAA CAATACCTCC AATTCGGGTG GAGGAGGAGG AGGTGGCGGA 
GGAGGAGGCT CAGGGGGTAA CTCCAATCTG TGCGGGCCTC GCTCTCGGCC CTCACGCATC 
CCGCAGCCTT CCTCGCGCCT CCCCCAGCCT GTTCACCACC ACCACCCCCC AGGCCCCGAG 
GGGCCTGACA GATCAGCAGG TATGTGGTCT CCCTGCCACC CGCAGCCCCC CGATTCCTTT 
CCCTCTAACT GCTTTTCTGA CAGCGCAACA AACGGAGAGC TGCCTGCCTC CGAGGTCCCT 
AAGATGAGGA TGGACAGTCC TCACGGAAGC AGCTGTAGTA ACAGTAACAG GCCGTTCAGT 
AATGCGGACA GCAGTGTCAG AAAGGGAGGA TGTGAGGAGA CGCAGAGGCC TCAGACCGCT 
GCGAAACCAC AGATGACAGT GGTTCCTTTG AATTTTCCTC TAAAAAGCCC CCGAGTCGGC 
ACCGTTTCCC CCCTAATGTC ATCTCCAGCT GCTGGAGCAG CAAAGGAACT GATCCTCCCT 
TCAAGCCCAG TGCATAAAGG AAACCCTTTC TGGGCTGTAG TCCCCCCTCT GCCTCCTTCG 
CCCGCCAGCA GGCCCGGTTC CTGCTCCTTC CCCAGTGACA ATAGTGGTGG AGGGGACTCT 
TTGGGCAGGG GAAGTCACGG GACGCCATCC CATCACCGCC ACTCCAGCCA TAATAAGGAT 
GTAGACCGCA TGAGCACCTG CTCCTCCACC AGCGAGCAGT CTGTTCACTC CACCCAGAGC 
AACGGGAGTG AAAGCAGTAG CAGCAGCAGC GTGTCCACCA TGTTGGTGAC CCAGGACTAT 
GTGGCAGTGA AGGAGGATGA GATCAGTGTG GCGCAGGGCG AGGTGGTCCA GATGCTTGCC 
AGCAACCAGC AGAACATGTT CCTGGTATAC CGGGCTGCCA ACGAGCACAG CCCTGCGGCT 
GAGGGCTGGA TCCCTGGCTA CGTTTTAGGC CACACCTCAA CCTCCGTCAC ACCCGAGCTT 
CCTGAAGGCA CCATCAAAAA ATCATTATCA TGGCACACAG CCCTCCGCAT CCGCCGAAAG 
TCTGAGAAAA GAGACAAGGA GGGGAAAAAA GTGGAGAATG GCTACCGCAA GTCTCAGGAC 
AGCCTGGCGA ACAAAGTCTC TGTCAAGCTT CTCAATCCCA ACTTCATTTA TGAGGCTCCA 
CCAGAGTTCC TCGTCCCTGT GAGCGATGCA TTATGTGAGA GCGGCGACAA CGTCATCCTG 
AGGTGTAAAG TATGTGGCCG TCCCCGAGCC ACCGTCACCT GGCACGGGCC GGACAATGAC 
ACCCTGAGCA GCGACGGACA CCACAGCATC ACATACAGTG AGACAGGTGA GGCATCTCTG 
CGCATCCTGG GAGCGTCTCC AGAGGATAGT GGGATGTACA CCTGTGTTGC CACGAACATC 
GCAGGCACCA GCTCCTCTTC TGCCAGTCTC ACAGTTTCAG GATCCCCCGA TGATGAAAGC 
GAAATCGTCT GGAAAAGCAG CTTTGAGTCC TATTTTACAG AGATAACTGA ACTGGGAAGG 
GGCCGCTTCT CAGTGACTAA GCGTTGTGAC CAGAGAGGAA GCAAACGAAC TGTAGCAGCA 
AAACACGTCA ACAAGAAGCT GCTGCGTCGT GAGAGGGCGC TGAAGGAGGT CCAACTCCTC 
CAGACTCTGG ACCATCCTAA CCTGGTCAAA CTGCTGGACA CATATGAGAC AGCCAACAGC 
TACGTGCTCA TCCTGGAGAT GGCAGACCAG GGCCGATTCC TGGATTACAT TGTGAGCTGG 
GGGAACCTGA CTGAAGAGAA GGTGGCTTTA TACCTTAGAG ACATTCTTGA GGCTCTTCAC 
TACCTGCACA GCTGGAGGAT AGCACACCTC GATCTCAAAC CTGAGAACAT TGTGGTGGAG 
CATGCATCCT CCCAGCCTGT AATCAAGCTG ACAGACTTTG GTGATGCCGT GCAGCTGAAC 
TCTGGTTGCT CCTATGTGCA TCCTCTGCTG GGAAGCCCCG AGTTCTCTGC TCCTGAGCTG 
GTTCTGGGTC AGCCCGCTTC CCTGATGTCT GACCTCTGGA GTTTAGGTGT GGTGACCTAC 
GTTGTCCTGA GTGGCGCCTC TCCCTTCCTG GACGAAAGCC TGGAAGAGAC GTGCCTGAAC 
ATCTGCCGCC TGGACTTCAG CTTCCCCGAA GATTACTTCC AGGGTGTGAG CCCAGCTGCC 
AGGGACTTTG TGTGCCGGCT GCTCCAAAGC GAGCCGGAAC GACGCCCCTC TGCGGCGTCC 
TGCCTCCAGG ATCCATGGCT GCAGCCCCGA GACGGCGGTC GCGACTCATC AAACCACACG 
CAGCCACACG CATTGCCACC ATCGCCGACC TTTCACAGCG TGCACCTGGA CACCTCCCGG 
CTTATCTCCT TCATTGAGAG ACGGAAGCAT CAGAACGACA TTCGGCCCGT CGGCGCCATC 
AAGGTCTTCC TTCACAGCCG CCTTCTCAAC CACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023820062.1
CDS	219..9677
Translation	MSAGEGLDEAAKDAADIAAFFKSDCSLPKCESKLDGPGQTSGFHRNEDLKAIDVLPILKEKVAFLSGGRDRRGGPILTFPARSNHDRIRPEDLRRLVAYLATIPSEEVARHGFTVIVDMRGSKWDSIKPLLKILQESFPTCIHVALIIKPDNFWQKQRTNFGSSKFEFETVMVSLEGLTKIVDPSQLTADFEGSLEYNHDDWIEVRLSFETFASDAARALSRLEELQEVLSQRDLPRDLEGARRLMEEHAALKKRATKASIEELDTQGRRLLQRLQSHSAGGGNSSESGYGNRGVGASGDSNDHHHGLQGYSDAHSLVAKVTALLDKLHGTRQNLQQLWHMRKLKLDQCFQLRLFEQDAEKMFDWIMHNKGLFLTSYTEIGGNHQHAVELQTQHNHFAMNCMNVYVNINRIMSVGNRLLESGHYASQQIQQISGQLEQEWKAFAAALDERSTLLEMSASFHQKADQYMSKVEPWCKACGEGELPTELQDLEDTIHHHQGLYEHITTAYSEVSQEGKSLLDKLQRPLTPGSADSLMSSANYSKAVHHVLDIIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKVLLDMSVAFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQATVNVIKEGEDLIQQLRDSAISSNKTPHNSSMAHIESVLQQLDEAQGQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNEELSQQMNDFDTEDLTLAEQRLQHHADKALTMNNLTFDVIHQGQELLQYVTEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAADQHRRHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQKEHEQFQHAIEKTHQSALQVQQKAEALLQANHYDMDMIRDCAEKVADHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSESDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMLSHVKAPETQVKNILNELLQRENRVLHFWTMRKRRLDQCQQYVVFERSAKQALEWIHDTGEFYLSTHTSTGSSIHHTQELLKEHEDFQITAKQTKERVKLLIQLADGFCDKGHAHALEIKKWVSSVDKRYRDFSIRMDKYRTCLETALGISSDSNKASKGLQLDIIPASAPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPHGIINKEHIIFGNMQDLYEFHHNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVNYCKNKPDSTQLIVEHAGAYFDEIQQRHRLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLDGFDGNIDSQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSNGHSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSLENKQDWIKHIREVIQERTVHLRGALKEPIHIPKATATKHKGRRDGEDLDSQGDASSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFVASNGGSNGELTVRRGQTVEVLERLHDKPDWCLVRTTDRSPAQEGIVPCSMLCIAHSRSSMEMEGLFNHKDTLSVSSNDALQPGSSQTLGPHSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHIKKLAHKHKKNREGTRKNMEVIPGSQKDSDDSAATPQDETLEERMRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKSASRLSARPSSSETPSAAELVSAIEELVKSKMSLEDRPSSLSVEQGDSSSPSLNPSDNSLLSSSSPTDEMDERKSGFLKRRHYVLLELVETERDYVRDLGSVVEGYMSRMKEEGVPDDMRGKDKIVFGNIYQIYDWHKDFFLGELEKCLEDPERLATLFVKQERRLHMYIVYCQNKPKSEHIVSEYIDTYFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDLLKVSKKAGVDTAELEKAVEVMCVVPKRCNDMMNVGRLQGFEGKIVAQGRLLLQDTFMVSDQDGGLLSRMKERRVFLFEQIVIFSEPLDKKKGFSTPGYLFKNSIKVSWLGLEENTDDPCKFTLTSRSSAGSMERYTLHSASPGVCQVWVHQVSQILENQRNFLNALTSPIEYQRNHVGGGGSSGPPSSSSSSAGGLPGGSSTNNTSNSGGGGGGGGGGGSGGNSNLCGPRSRPSRIPQPSSRLPQPVHHHHPPGPEGPDRSAGMWSPCHPQPPDSFPSNCFSDSATNGELPASEVPKMRMDSPHGSSCSNSNRPFSNADSSVRKGGCEETQRPQTAAKPQMTVVPLNFPLKSPRVGTVSPLMSSPAAGAAKELILPSSPVHKGNPFWAVVPPLPPSPASRPGSCSFPSDNSGGGDSLGRGSHGTPSHHRHSSHNKDVDRMSTCSSTSEQSVHSTQSNGSESSSSSSVSTMLVTQDYVAVKEDEISVAQGEVVQMLASNQQNMFLVYRAANEHSPAAEGWIPGYVLGHTSTSVTPELPEGTIKKSLSWHTALRIRRKSEKRDKEGKKVENGYRKSQDSLANKVSVKLLNPNFIYEAPPEFLVPVSDALCESGDNVILRCKVCGRPRATVTWHGPDNDTLSSDGHHSITYSETGEASLRILGASPEDSGMYTCVATNIAGTSSSSASLTVSGSPDDESEIVWKSSFESYFTEITELGRGRFSVTKRCDQRGSKRTVAAKHVNKKLLRRERALKEVQLLQTLDHPNLVKLLDTYETANSYVLILEMADQGRFLDYIVSWGNLTEEKVALYLRDILEALHYLHSWRIAHLDLKPENIVVEHASSQPVIKLTDFGDAVQLNSGCSYVHPLLGSPEFSAPELVLGQPASLMSDLWSLGVVTYVVLSGASPFLDESLEETCLNICRLDFSFPEDYFQGVSPAARDFVCRLLQSEPERRPSAASCLQDPWLQPRDGGRDSSNHTQPHALPPSPTFHSVHLDTSRLISFIERRKHQNDIRPVGAIKVFLHSRLLNHS

Target ORF information:

RefSeq Version	XM_023964294.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes triple functional domain protein (LOC101164837), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023964294.1

ORF Insert Sequence:

ATGAGCGCAG GAGAGGGTCT GGATGAGGCT GCGAAGGACG CGGCAGACAT AGCGGCGTTT 
TTCAAATCCG ATTGCTCTCT TCCAAAGTGT GAATCCAAAC TGGACGGCCC CGGTCAAACA 
TCAGGGTTCC ATCGCAACGA AGATTTGAAA GCCATTGATG TGCTTCCCAT CCTAAAAGAG 
AAGGTCGCCT TTCTTTCAGG TGGCAGAGAC AGACGTGGGG GTCCAATTCT CACTTTTCCA 
GCACGGAGCA ACCATGACAG AATACGACCC GAGGACCTGC GTAGGCTTGT AGCATACTTG 
GCTACTATTC CAAGTGAGGA GGTGGCCAGA CATGGCTTCA CCGTCATCGT GGACATGCGT 
GGTTCTAAAT GGGACAGCAT CAAACCGTTG CTGAAAATCC TACAGGAGTC TTTTCCCACC 
TGCATCCACG TTGCCCTCAT CATCAAACCG GACAACTTCT GGCAAAAACA GAGAACCAAC 
TTTGGCAGTT CTAAGTTTGA ATTTGAAACG GTGATGGTCT CATTAGAGGG CTTAACCAAA 
ATAGTGGACC CATCGCAGCT GACAGCTGAC TTTGAGGGCA GCTTAGAGTA CAACCATGAC 
GACTGGATCG AGGTGCGGCT ATCATTCGAG ACATTTGCCA GTGATGCAGC GAGGGCTTTG 
TCACGCCTCG AGGAGCTCCA GGAGGTCCTG TCCCAGCGTG ATCTCCCGCG GGACCTGGAA 
GGAGCCCGCC GCCTCATGGA AGAGCACGCT GCGCTGAAGA AAAGGGCCAC AAAGGCGTCC 
ATAGAGGAGC TTGACACGCA GGGCCGACGG CTGCTCCAAA GACTGCAGTC TCATTCTGCA 
GGGGGTGGAA ACAGCAGTGA AAGCGGATAC GGCAACCGCG GCGTGGGGGC CAGTGGAGAT 
TCCAACGACC ATCACCACGG GCTCCAGGGT TACAGCGACG CACACAGTCT AGTGGCTAAA 
GTTACGGCTT TGTTGGATAA GCTGCATGGG ACGCGGCAGA ACTTGCAGCA GCTGTGGCAC 
ATGAGGAAGC TAAAACTCGA CCAGTGTTTC CAGCTGCGGC TGTTTGAGCA AGATGCTGAG 
AAGATGTTCG ACTGGATTAT GCACAACAAG GGGCTTTTTC TTACCAGCTA CACTGAGATT 
GGGGGGAACC ACCAGCATGC TGTTGAGCTG CAGACACAGC ACAATCATTT TGCTATGAAC 
TGCATGAATG TCTATGTGAA CATCAACCGA ATCATGTCGG TGGGAAACCG ACTCCTGGAG 
TCTGGTCACT ACGCCTCCCA GCAGATCCAG CAGATCTCCG GCCAGCTGGA GCAGGAATGG 
AAAGCCTTTG CTGCAGCCCT GGATGAACGC AGCACACTGC TGGAGATGTC TGCCAGCTTC 
CATCAGAAGG CAGATCAGTA CATGAGTAAA GTGGAGCCAT GGTGCAAGGC ATGTGGAGAG 
GGAGAGCTGC CCACCGAACT CCAGGACCTG GAGGATACAA TCCACCATCA CCAGGGACTC 
TACGAGCACA TCACCACGGC ATACTCAGAG GTAAGCCAGG AAGGCAAATC TTTACTGGAC 
AAGCTGCAGC GACCGTTGAC CCCAGGAAGT GCCGATTCGT TGATGTCCTC GGCCAACTAC 
TCCAAGGCTG TGCACCACGT CCTGGATATC ATCCACGAGG TTTTGCACCA TCAGAGACAA 
CTGGAAAACA TATGGCAGCA TCGCAAAGTC CGACTTCATC AGCGGCTGCA GCTTTGTGTG 
TTCCAGCAGG ACGTGCAGCA GGTTTTGGAT TGGATCGAAA ATCATGGCGA GGCTTTCCTA 
AGTAAGCACA CCGGCGTGGG GAAATCTCTG CACAGAGCCA GGGCTCTGCA GAAACGACAC 
GAGGACTTTG AAGAAGTTGC CCAGAACACC TACACTAATG CTGACAAGTT GCTGGAGGCA 
GCAGAGCAGC TGGCCCAAAC TGGAGAGTGT GACCCAGAGG AAATATACCA GGCCGCCCAC 
CAGCTAGAAG ACCGCATCCA AGATTTTGTG CGGCGTGTGG AGCAGCGTAA AGTCTTGCTG 
GACATGTCTG TTGCATTTCA CACGCATGTC AAAGAGCTGT GGACGTGGTT GGAGGAGCTG 
CAGAAGGAAC TGCTGGACGA TGTGTATGCG GAGTCCGTGG AGGCGGTTCA GGACTTGATC 
AAGCGCTTCG GCCAGCAGCA GCAGACCACG CTGCAGGCAA CCGTCAATGT TATCAAAGAG 
GGAGAGGACC TTATACAGCA GCTCAGAGAC TCGGCTATCT CCAGCAACAA GACCCCTCAC 
AACAGCTCCA TGGCCCACAT CGAGAGCGTG CTGCAGCAGC TGGATGAAGC CCAGGGCCAG 
ATGGAAGAGC TGTTCCAGGA AAGGAAGATC AAGCTGGAGC TGTTTCTCCA ACTACGCATA 
TTTGAGAGGG ATGCCATTGA TATCATCTCC GACCTGGAGT CATGGAACGA GGAACTGTCC 
CAGCAGATGA ACGACTTCGA TACAGAGGAC CTGACTCTGG CAGAGCAGAG GCTGCAGCAT 
CACGCTGACA AAGCGCTCAC CATGAATAAC CTGACCTTTG ACGTCATTCA CCAGGGGCAG 
GAGCTGCTTC AGTACGTCAC CGAGGTCCAG GCGTCCGGTG TGGAGCTGCT ATGCGACAGA 
GACGTGGACA TGGCCACACG GGTTCAGGAC CTGCTGGAGT TTCTTCATGA GAAGCAGCAG 
GAGCTGGACT TGGCTGCAGA CCAGCACAGG AGGCACTTGG AGCAGTGTGT CCAGTTAAGG 
CACCTGCAAG CTGAAGTTAA ACAGGTGCTG GGTTGGATCC GCAATGGGGA GTCGATGTTG 
AATGCAGGTC TAATAACGGC TAGCTCCTTG CAAGAAGCTG AGCAGCTGCA GAAGGAGCAC 
GAACAGTTCC AGCATGCAAT AGAGAAAACA CACCAAAGTG CTCTGCAGGT GCAGCAGAAG 
GCAGAAGCTC TTCTCCAGGC CAACCACTAC GACATGGACA TGATCAGAGA CTGTGCTGAA 
AAGGTGGCCG ACCACTGGCA GCAGCTGATG CTGAAGATGG AGGACAGACT TAAACTGGTC 
AATGCTTCTG TGGCCTTCTA TAAAACATCA GAACAGGTGT GCAGTGTGTT GGAAAGTCTG 
GAGCAGGAGT ACAAGCGGGA GGAGGACTGG TGTGGTGGGG CTGATAAACT GGGCCCAAAC 
AGTGAGTCAG ATCACGTCAC ACCTATGATC AGCAAACATC TGGAGCAGAA AGAAGCTTTT 
CTTAAGGCCT GCACATTAGC CAGACGCAAT GCTGATGTTT TCCTGAAGTA TCTGCACAGA 
AACAGCGTCA ACATGCCGGG CATGTTATCT CATGTCAAAG CACCAGAGAC TCAGGTCAAG 
AACATTTTGA ATGAGTTGCT TCAGAGAGAA AACAGAGTCC TCCACTTCTG GACGATGAGG 
AAGCGGAGGT TGGACCAGTG CCAGCAATAT GTAGTGTTTG AAAGAAGTGC CAAACAGGCC 
TTGGAATGGA TCCACGACAC CGGCGAGTTT TACCTGTCCA CTCATACATC CACTGGCTCC 
AGCATTCATC ACACACAGGA GCTACTGAAA GAGCACGAAG ACTTCCAGAT CACAGCAAAG 
CAAACCAAAG AGCGGGTAAA GCTGTTGATC CAGCTGGCGG ATGGGTTTTG TGACAAGGGC 
CACGCCCATG CCCTGGAAAT AAAGAAATGG GTTTCCTCGG TGGACAAGCG CTACAGAGAC 
TTCTCTATCC GTATGGACAA ATACCGAACC TGCCTGGAAA CGGCGCTGGG CATCTCGTCT 
GACTCAAACA AGGCTAGTAA AGGACTGCAG TTAGACATAA TCCCAGCTAG TGCTCCAGGG 
TCAGAGGTCA AGTTGAGGGA TGCCGCTCAT GAACTGAACG AGGAGAAGCG GAAATCTGCA 
CGACGAAAAG AATTTATAAT GGCTGAGCTG ATTCAAACAG AGAAAGCATA CGTGAGAGAT 
CTGCGAGAGT GCATGGATAC GTACCTGTGG GAAATGACCA GTGGTGTGGA GGAGATTCCC 
CATGGCATCA TCAACAAGGA ACACATCATC TTTGGGAACA TGCAGGACCT CTATGAGTTT 
CATCACAACA TTTTCCTCAA AGAGCTGGAG AAGTATGAGC AGCTTCCAGA GGATGTTGGC 
CATTGCTTTG TGACCTGGGC TGATAAGTTC CAGATGTATG TGAACTACTG TAAGAACAAG 
CCAGACTCAA CTCAACTCAT TGTTGAGCAT GCGGGAGCCT ACTTCGATGA AATTCAGCAA 
AGACATCGTC TTGCCAATTC CATCTCGTCC TACCTGATCA AGCCGGTTCA GCGAATCACC 
AAATACCAAC TGCTTCTCAA GGAGTTGCTA ACATGCTGTG AGGAGGGAAA AGGTGAAATA 
AAAGATGGTC TGGAAGTGAT GCTCAGTGTT CCCAAGAGAG CCAATGATGC CATGCATCTC 
TCAATGCTGG ACGGTTTCGA TGGGAACATT GACTCCCAAG GAGAGTTGAT CCTCCAAGAG 
TCCTTCCAGG TGTGGGACCC CAAGACTCTG ATTCGGAAAG GTCGTGAAAG GCACCTCTTC 
CTCTTTGAGA TGTCTCTCGT CTTCAGCAAA GAAGTAAAAG ATTCCAACGG ACACAGCAAA 
TACCTCTACA AGAGCAAGCT CTTTACCTCT GAATTGGGTG TGACAGAGCA TGTGGAGGGA 
GATCCTTGTA AGTTTGCCCT GTGGGTGGGC AGGACCCCAA CCTCAGACAA CAAGATTGTT 
CTTAAGGCCT CAAGTCTAGA GAATAAGCAG GACTGGATCA AACACATCAG AGAGGTCATC 
CAAGAGCGAA CCGTCCACCT GCGCGGAGCT CTGAAGGAGC CCATTCACAT CCCAAAAGCC 
ACAGCTACCA AACACAAGGG CAGACGGGAT GGAGAGGATC TGGACAGCCA AGGAGATGCC 
AGCAGCCAAC CAGACACCAT TTCCATCGCC TCTCGCACAT CTCAGAACAC GCTGGACAGT 
GACAAGCTCT CGGGCGGCTG TGAGCTGACG GTGGTAATCC ACGACTTTGT GGCCAGCAAT 
GGGGGTAGCA ACGGGGAACT AACAGTCCGG CGGGGCCAAA CTGTGGAGGT TCTGGAGCGG 
CTGCACGACA AGCCGGACTG GTGCCTGGTC CGAACCACAG ATCGGTCCCC TGCCCAGGAA 
GGCATAGTGC CCTGCTCCAT GCTCTGCATC GCCCACTCCC GCTCTTCCAT GGAGATGGAG 
GGGCTGTTCA ACCACAAAGA CACCTTGTCT GTATCGAGTA ATGACGCCCT GCAGCCAGGC 
TCCAGCCAAA CTTTGGGCCC CCACAGCTCA CCCGGACCCA AACGTCCCGG GAACACCTTA 
AGGAAATGGT TGACGAGCCC CGTACGCCGA CTCAGCTCGG GCAAAGCCGA TGGTCACATC 
AAAAAGCTGG CCCACAAGCA CAAAAAGAAC CGCGAAGGCA CCAGGAAGAA CATGGAAGTG 
ATCCCGGGGT CGCAGAAGGA CTCCGACGAC AGCGCCGCCA CTCCACAGGA CGAGACTTTG 
GAGGAACGCA TGCGCAACGA AGGGCTAAGC AGCGGCACCC TCTCAAAGTC TTCCTCATCG 
GGGATGCAGA GCTGCGGCGA GGAGGAGGGA GAGGAGGGTG CGGACGCTGT TCCTCTGCCT 
CCCCCGATGG CCATCCAGCA GCACAGCCTG CTGCAGCCTG ACTCCCAGGA TGACAAGTCT 
GCGTCTCGTC TGTCCGCGCG TCCTAGTAGT TCAGAGACAC CCAGTGCTGC AGAATTGGTC 
AGTGCCATCG AGGAGTTGGT GAAGAGCAAG ATGTCGCTGG AGGACCGGCC CAGTTCTCTG 
TCCGTGGAGC AAGGCGACAG CAGCTCACCC TCTCTCAACC CCTCCGACAA CTCACTCCTC 
TCCTCGTCCT CACCCACGGA CGAGATGGAC GAGCGCAAAT CTGGCTTCCT CAAGCGAAGG 
CACTATGTGC TCCTTGAGTT AGTGGAGACT GAAAGGGACT ACGTCAGGGA CCTTGGATCT 
GTTGTGGAGG GCTACATGAG CAGAATGAAA GAGGAGGGAG TTCCAGATGA CATGAGAGGA 
AAAGACAAGA TTGTTTTTGG AAACATCTAC CAGATCTACG ACTGGCACAA AGATTTTTTC 
CTGGGAGAGC TGGAGAAGTG TTTGGAAGAC CCCGAGAGGC TGGCTACTTT ATTTGTCAAA 
CAGGAGCGGA GGCTGCACAT GTACATCGTG TACTGCCAGA ACAAGCCCAA ATCCGAGCAC 
ATTGTGTCCG AGTATATTGA CACCTATTTT GAAGACCTAA AGCAGCGACT TGGTCACAGA 
CTGCAGCTCA CCGATTTGCT AATTAAACCA GTCCAGCGCA TAATGAAGTA CCAGCTGCTG 
CTAAAGGATC TACTCAAAGT TTCTAAAAAG GCAGGAGTGG ACACAGCAGA GCTGGAGAAA 
GCTGTTGAGG TGATGTGCGT GGTCCCAAAG CGTTGCAACG ACATGATGAA TGTTGGACGT 
CTTCAAGGCT TTGAAGGGAA AATTGTTGCT CAGGGCAGGC TGCTCCTCCA AGACACCTTC 
ATGGTTTCAG ACCAAGATGG GGGTCTACTG TCAAGGATGA AAGAAAGGAG AGTGTTTTTG 
TTTGAGCAGA TAGTTATATT CAGCGAACCT CTGGATAAAA AGAAGGGCTT CTCCACTCCT 
GGCTACCTCT TTAAAAACAG CATCAAGGTG AGCTGGCTGG GTCTGGAAGA AAACACAGAC 
GACCCCTGCA AGTTTACATT AACTTCAAGG TCATCTGCTG GGAGCATGGA GAGGTACACG 
CTTCACTCCG CCAGCCCAGG AGTGTGCCAA GTCTGGGTTC ACCAGGTCAG CCAGATCTTG 
GAAAATCAGC GGAACTTCCT CAACGCTTTG ACATCCCCCA TAGAGTACCA GAGGAACCAC 
GTGGGTGGAG GAGGGTCAAG CGGACCCCCT AGTAGTAGCA GCAGTAGTGC CGGAGGCCTC 
CCAGGAGGCA GCAGCACCAA CAATACCTCC AATTCGGGTG GAGGAGGAGG AGGTGGCGGA 
GGAGGAGGCT CAGGGGGTAA CTCCAATCTG TGCGGGCCTC GCTCTCGGCC CTCACGCATC 
CCGCAGCCTT CCTCGCGCCT CCCCCAGCCT GTTCACCACC ACCACCCCCC AGGCCCCGAG 
GGGCCTGACA GATCAGCAGG TATGTGGTCT CCCTGCCACC CGCAGCCCCC CGATTCCTTT 
CCCTCTAACT GCTTTTCTGA CAGCGCAACA AACGGAGAGC TGCCTGCCTC CGAGGTCCCT 
AAGATGAGGA TGGACAGTCC TCACGGAAGC AGCTGTAGTA ACAGTAACAG GCCGTTCAGT 
AATGCGGACA GCAGTGTCAG AAAGGGAGGA TGTGAGGAGA CGCAGAGGCC TCAGACCGCT 
GCGAAACCAC AGATGACAGT GGTTCCTTTG AATTTTCCTC TAAAAAGCCC CCGAGTCGGC 
ACCGTTTCCC CCCTAATGTC ATCTCCAGCT GCTGGAGCAG CAAAGGAACT GATCCTCCCT 
TCAAGCCCAG TGCATAAAGG AAACCCTTTC TGGGCTGTAG TCCCCCCTCT GCCTCCTTCG 
CCCGCCAGCA GGCCCGGTTC CTGCTCCTTC CCCAGTGACA ATAGTGGTGG AGGGGACTCT 
TTGGGCAGGG GAAGTCACGG GACGCCATCC CATCACCGCC ACTCCAGCCA TAATAAGGAT 
GTAGACCGCA TGAGCACCTG CTCCTCCACC AGCGAGCAGT CTGTTCACTC CACCCAGAGC 
AACGGGAGTG AAAGCAGTAG CAGCAGCAGC GTGTCCACCA TGTTGGTGAC CCAGGACTAT 
GTGGCAGTGA AGGAGGATGA GATCAGTGTG GCGCAGGGCG AGGTGGTCCA GATGCTTGCC 
AGCAACCAGC AGAACATGTT CCTGGTATAC CGGGCTGCCA ACGAGCACAG CCCTGCGGCT 
GAGGGCTGGA TCCCTGGCTA CGTTTTAGGC CACACCTCAA CCTCCGTCAC ACCCGAGCTT 
CCTGAAGGCA CCATCAAAAA ATCATTATCA TGGCACACAG CCCTCCGCAT CCGCCGAAAG 
TCTGAGAAAA GAGACAAGGA GGGGAAAAAA GTGGAGAATG GCTACCGCAA GTCTCAGGAC 
AGCCTGGCGA ACAAAGTCTC TGTCAAGCTT CTCAATCCCA ACTTCATTTA TGAGGCTCCA 
CCAGAGTTCC TCGTCCCTGT GAGCGATGCA TTATGTGAGA GCGGCGACAA CGTCATCCTG 
AGGTGTAAAG TATGTGGCCG TCCCCGAGCC ACCGTCACCT GGCACGGGCC GGACAATGAC 
ACCCTGAGCA GCGACGGACA CCACAGCATC ACATACAGTG AGACAGGTGA GGCATCTCTG 
CGCATCCTGG GAGCGTCTCC AGAGGATAGT GGGATGTACA CCTGTGTTGC CACGAACATC 
GCAGGCACCA GCTCCTCTTC TGCCAGTCTC ACAGTTTCAG GATCCCCCGA TGATGAAAGC 
GAAATCGTCT GGAAAAGCAG CTTTGAGTCC TATTTTACAG AGATAACTGA ACTGGGAAGG 
GGCCGCTTCT CAGTGACTAA GCGTTGTGAC CAGAGAGGAA GCAAACGAAC TGTAGCAGCA 
AAACACGTCA ACAAGAAGCT GCTGCGTCGT GAGAGGGCGC TGAAGGAGGT CCAACTCCTC 
CAGACTCTGG ACCATCCTAA CCTGGTCAAA CTGCTGGACA CATATGAGAC AGCCAACAGC 
TACGTGCTCA TCCTGGAGAT GGCAGACCAG GGCCGATTCC TGGATTACAT TGTGAGCTGG 
GGGAACCTGA CTGAAGAGAA GGTGGCTTTA TACCTTAGAG ACATTCTTGA GGCTCTTCAC 
TACCTGCACA GCTGGAGGAT AGCACACCTC GATCTCAAAC CTGAGAACAT TGTGGTGGAG 
CATGCATCCT CCCAGCCTGT AATCAAGCTG ACAGACTTTG GTGATGCCGT GCAGCTGAAC 
TCTGGTTGCT CCTATGTGCA TCCTCTGCTG GGAAGCCCCG AGTTCTCTGC TCCTGAGCTG 
GTTCTGGGTC AGCCCGCTTC CCTGATGTCT GACCTCTGGA GTTTAGGTGT GGTGACCTAC 
GTTGTCCTGA GTGGCGCCTC TCCCTTCCTG GACGAAAGCC TGGAAGAGAC GTGCCTGAAC 
ATCTGCCGCC TGGACTTCAG CTTCCCCGAA GATTACTTCC AGGGTGTGAG CCCAGCTGCC 
AGGGACTTTG TGTGCCGGCT GCTCCAAAGC GAGCCGGAAC GACGCCCCTC TGCGGCGTCC 
TGCCTCCAGG ATCCATGGCT GCAGCCCCGA GACGGCGGTC GCGACTCATC AAACCACACG 
CAGCCACACG CATTGCCACC ATCGCCGACC TTTCACAGCG TGCACCTGGA CACCTCCCGG 
CTTATCTCCT TCATTGAGAG ACGGAAGCAT CAGAACGACA TTCGGCCCGT CGGCGCCATC 
AAGGTCTTCC TTCACAGCCG CCTTCTCAAC CACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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LOC101164837 cDNA ORF clone, Oryzias latipes(Japanese medaka)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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