LOC101162913 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101162913
Entrez Gene ID	101162913
Full Name	uncharacterized LOC101162913
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011473328.3	XP_011471630.1	uncharacterized protein LOC101162913 isoform X1
XM_011473328.2	XP_011471630.1	uncharacterized protein LOC101162913
XM_020701850.1	XP_020557509.1	uncharacterized protein LOC101162913
XM_020701850.2	XP_020557509.1	uncharacterized protein LOC101162913 isoform X1
XM_023964092.1	XP_023819860.1	uncharacterized protein LOC101162913 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC101162913 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101162913 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa27422	XM_011473328.3 Latest version!	Oryzias latipes uncharacterized LOC101162913 (LOC101162913), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OJa27422	XM_020701850.2 Latest version!	Oryzias latipes uncharacterized LOC101162913 (LOC101162913), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OJa27423	XM_023964092.1 Latest version!	Oryzias latipes uncharacterized LOC101162913 (LOC101162913), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OJa27422	XM_011473328.2	Oryzias latipes uncharacterized LOC101162913 (LOC101162913), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OJa27422	XM_020701850.1	Oryzias latipes uncharacterized LOC101162913 (LOC101162913), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101162913 ( XM_011473328.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011471630.1 Oryzias latipes uncharacterized LOC101162913 (LOC101162913), transcript variant X1, mRNA.

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CloneID	OJa27422
Clone ID Related Accession (Same CDS sequence)	XM_011473328.3 , XM_011473328.2 , XM_020701850.1 , XM_020701850.2
Accession Version	XM_011473328.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	uncharacterized protein LOC101162913 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019874.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_011473328.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGTC CATCTTTGAC CCGGACGGAC TCCATCCCTG CCTCCTCATC GGCCTCAGAG 
CAGCCCGTGG ACATGGTGGC TGAGCCGGAG CAGCAGGGAC CAGAGGGACA GGCGGGGGAC 
CACCCGGCAG TGGCTCCTGC AGACCCCCAA CCACCAGAGA CGAGTCAACA CACCCAAGGG 
GGAGCACAGA GCCCTGCAAA TGTAGCGGCC ATGCCAACAG CAGAGGCTGA AGATGATGCC 
AAGAGAAGCA CTGAGAGCAG CAGTGGAGCG AAGACAAACG TGCAGACCGT CAGGAGATAC 
GTCCCTTCCA AGAAAGCAAT GATAGATCCT CTGAAGATGG ATATGACCAA ATCGCTGGTT 
GAGCCACTCT CATCCTCTCA GCTCTCTCTG CACTGCGTCG AGTGCCACAT CATCTTCAGT 
GACCACAAGA GCAAAGACCG CCACGTGAAG CTGAAACACC CAGTAGAATA TGAAAAGCGC 
AACCTGAAAA ACGCCCTTTT TGCATGTTAC GTGTGTGACC GCCATTTTAC CAACTCCACG 
GACCTCATGG CCCACCAGAA GGCCCACACT GAGAAGAAAC CCTTCAAGTG TCCCGTGTGC 
GATCAGGCCT TCAGGAGGTC CTCCGAGCTC ACCAGTCATA AAAAAGCTCA TTTCGGCCTG 
TTCGGCTACA CCTGCACGGA GTGCGGCAAA CCCTGCAAAA CGCTGACATT GCTGAAGTAT 
CACCAGCGTA CGCACACAGG AGAGAGGCCG TACGTCTGCA AGGAGTGTGG GAAGAGGTTC 
CCCATGCCGA AAGCTTTGCA CAAACACATG ATCTCCCACT CTCCAGAGGG AGCTGAAGGA 
GAAGAAAAAG ACGACGCTGC CAAAAAACGA GCAAAGAAAG ATGAAGGTTC TCCTCCTGTC 
AAACTCCACT GCTCCATGTG CAAGGCCACT TTCAAGAACT CCAAGACTCG CCAGCGGCAC 
ATGAAAATCA AGCACAACGT CCTCCCATCA GGGCAAAGCA AACCTGTTAT CACCCCCATT 
TCGATCTCAC AGCCCACGCG GTTGCAGCTA GAGGCCGCTG GACCTCTGCA AAAAGTTGAT 
GCCAACATCG ACACAGCGCA AATACGCAAA CTTATAGAGT CGCTGGGCAC TGTGAAGAAA 
GTGAACCAAG TGGTTCTGTT GGGTCAGGTG CCGGGTTACG CTCCACCACT GGAATTGCAG 
GCGGATTCGC AGCCAGCAGG GCCTCTGGAT CCCGATCTAA GTCCACCGCA GATCGATTTT 
GGAGGACTGA AGCCGCCCGA ATCCAAGACG ACTGGACTGG ACACTTCCAA TTATCCGTGT 
GGGTTCATGG ACCAAACCAT CATTCTGGAA CCCATCACTC CTGATGGAGA AACCCCCCCC 
CTGTCAGAGC TGGCATCCTG TGTAGCAGCA GGCGAAACTG CACAGACTGT GGGAGAGGTG 
GTTCAGAGGC CCCTGACCAG TGAAATTCTC TTGGATAATA TGACCTGCCA GAACGAGGAG 
AATGACCTTG AACAAACATT CATATTAGAA CTCACCCCAG CTTTGACACC AACTGCAGAA 
CTGGAGCACT CCCAGACTGA ACCACAGAAT ATGGCTCCGT CCTCCTCTCT GGTGCTGAAC 
ACGGACCAGG AGGCTGCAGA TCAAACTGCC AAAAAGTCTG CCCTCAATGG GCAAGACGCC 
AGCCTGACAG AACCGCCACT TGTCTCCACA GCCCAGCTGG ATGGAACATC TCCTTCCTTA 
GACCCAGAGC ACAAAGAACT TCCCTCCAAC CCTTTGGATT CACCAGATAA ACCCTCACAA 
GTTGGCCCCA ATGAAGGCGA TGATGCATGT ACGAAGATGA CGGGTCAAGA CCAGTTGAGT 
GAGGGCGAAT TAAAGACACA GGAACACAAT GGAGAGCATT TGTCTGAGAA ATCTGGAATG 
GAAGGTAAGA AGAACCATTC AGAAGTGGAG TCGTCTTCTA AAGCGGCAGT CCCGTCTGAC 
ATGCAGTCAA AACATGTTCC TCAGACCTCA GAACTGGCCC TGAATGTCAT GTCTGCTCAG 
GAGCTGGTGA AAGTGCGCAA AAGAAAGCCG GCCAGGACGT TTTTCTTTCC AGGATACATT 
CAAGATCTGG CCGGGTCCAT TTACAAAGAC GACTTTCTGA TCGACGCCAA ACCAGCCAAG 
CGGCAAAGAA CAAAAAAGTC TCATCTTGTT GTGAAATTCA GTCCTCAGAG CAAAGACAAG 
AAAAATAAGA AAAAAAAGCA GGAACCGCAG CAGCCAAGAC CAGAAGATCT GACTAAAGGT 
AAAGCTCCGG CTAAGAGAAC ACCTGAGAAA AAGATGTCCT TGTCACCAAA GAAAGGAGCA 
AAGGGAAAAA AGGATGCTGC AGGGGGGGGC GGAGCATCAG GAGTTGTCAC AAAGTCCCCT 
CCAACCCCAG TTGCACAGGA GAAACAAATC AAAGAAGATG TGGGTAAAAA CAAACTAAAA 
AAAAAAAAAG TAGGGAACGA AGATGCACAA ACGAGTGGGC AGAAGGCCGC AGCTTCACCT 
GTGTTCAAAA AGAAAAAGCA ACCAAAGGTT AGACAAGAAG TTTCACCCAA AAGTGCAAAA 
ACTGAAAGAG CTAGGAAAAA AACACCAACC AAAAAAGTCA AAAAGCCAGA AGAACCGAAT 
TTAAACGCAG TACAAGACTC TCTGCTTTTA TTAAAAGGCC ATAAGCAGCC CCAGCTCAAA 
GTTTACAAGC TGGACCCTTC CAGGGCGTCG AGTCAGCCTC AGGACGCCTC CGTCTCCAAG 
AAAAAGGGAG GACGGGCAAA GAAAGCTCTG TCCCTCATGT CTTCCTTGAA GTCTTCTCAA 
CCTCCACCCG ATGCTCCGCC CAACAAGCCA AAGGCCACCA GAAAACGCAA AGCCTCGTCG 
AAAGTGGAAA CTGAAGGAGT CATAACCTCT AAGCGAGCCT TGGAGTGCAA AGACTGTGGG 
GAAAAGTTCA GTGACGTCTC ATCGCTTCAG AAGCACAAAA CGACCGCACA CACCGTAGAG 
AGTCCAAGTC TCACCTACAC CAACGGAAAC ATATTTGAGG GCGTCTCCAG GTTGGAGTTT 
TACCCGTCCC CGAAGGTGTA TGACGGCGGA GCCGCGGGAA AGAATGCAGC AACAGGGTGG 
GACACTGAGC CAGAGATGGG AGAGATGACT GTGGAGGACA GGGAGCAGAA TGTCTCTTTC 
CCAGCTCTGA TTCCATCCCC ATCTCTGCCG GTTTCTCCTC CAAATGTTGA CATAAATGCC 
TTCCAGGGGG CAAGTAGAAG AGACGAGAAC AAAGCCTCCC CTTTAGATGT TTGCTCTCGG 
CCCCGTGACA TGACTGCTGG CAAGGATCGC ACAGATTTCC TAGAGTCCGC TCAGGCGAAG 
TCCTCTGAGA GGAGGCCGCT GTTTCCACCA GAATGTGCCT CAAAGAGCCC CAACTCTGAA 
TGTGAAGCCC AGGGAACCAC CGAGGAAGAC TTCAAGGAAG ATCTACTTCT AGAGGTTGAC 
TTGGTTACTG TTGGGGAACA GAATGAGAGA GACAGAAGTT CTCTGGATGA AGACACTTCT 
ACCCAAAAAG ATTTAAACAC CAGTGATTCT GAAAGTGATA AACACCCCGA GCAGATTTGT 
GATGGAAGGA GTGAAAATGA GGTAACTTCA AAGACTGTGT TTTGCTCAAC CAATCAGGTG 
AAAGAAGAAG AGGAAGAGAT GTTGGTGCAG AGGAGAGGAG AGGACCAGAA GAGCGTTGTG 
ATGAGTAGAC GTAGGAGAAG AGGCTCAGAG CCCAGAAGAG GAGATGCCAT AAGAAAGACG 
GGATCTGTTG GAGAGTCTCA GACGGAAACT GAAAGTGAGC AGGATGAATG TCAGGTGGTT 
TATGAGATAC ACTCCGTCTT CTCGGACCAG GAGTCTGACA CGAAGACCAG CCCTGGATTC 
AGGACCATGA AAGGCCCTGC TTCTGTTGCG TTCATCCCTT CTGGTCCTTC AGCGTTTGAC 
AAATCCTCTG AAGAGCGTGT AGTGCTGGAG CTGGAGTCTG TAACCACTAG TGTTCAGGAA 
GTGATGAACG AACACAGGCT GCATGGAGAC GGGGAGACGG ACAGGGAAGC AGACCAGTCT 
CCAGGCATCG TGCTGGAGAG GTTCCTGACC TCCAGACAAA GATCTTCTGA CAAAGAGGCC 
TCCTGCAGGG CTGCAGACCA ACGAGAGGGT TTTGATGGTG TTTCTGAGAA AGAAGTACAG 
TTTGTTGGGA GCCAGGAGAT CAAAGTTGAA GAGACCCCGC CGGACCCCAC ACTGCAGCCC 
CCTGCCTGTC AAAGCAGGCA GAGGGCCGGT CTCCATCCAC AGCATAATCG AGCCATACGG 
ACCGTTCTAG TGAAAGAGGA GAGCGGCCCC CTGCTGAACG CTGCTCTGGA CGCCCAAGGC 
AGCAGACGGA TGCAGTGGGC AGTGGAGCCA ATCAGCAGCG GGGGGGCCGA TGGGCCGTTA 
ATTGAGAATG GGCAGAGCCC CAATGAGGCA CGCAGGAGTC CAGAGTTCAA CACCAAGCAG 
TGCATCTTCT ATCCAGTGAA GGAGGAGGAG CGGGAACTTC TGCTGGCATC TGCTCAGACC 
AACGCTGACA CCTTCAGCCT GGAAGGCTTC GGTGATGGAG CCCCAGCTGA GCATCAACCT 
GCAGAGTGTG ATGCATATGG AGGAAGACTC TCTGCTGCAG GCTACCAGAA TGGAAGCCTG 
CTGTCAGAAC CAGAGACTTC AGACTTCACT GACAGTCAAG CTTTCTCTGA CTCGGAGTGG 
CAGCCGTCAG ATGTGCGGGA CTTTCTACTC AAGAGTTGTG ATGACGACGT GAGCGGCTTC 
GAACTGCCTG ATCCTCAGCG TGACACAGAA GCAGAAGTGA TGGCTTACTT CAACAGGAAG 
CAGTCCAGCA GCTCGCATGA GCCCAATCAC ACATCCGCGA GTTTTTCAAC ATCGAGTCAT 
CTGCAACCCC AGATGAATGG AACCAGGACC AGAGAGCCCA TTGATTACTT TTCCAAATAT 
TTTGGTTGGG ATACTTGGGC AGAAACTTCA ACTTGCACAA ACAAACTCTG CAAAATGTCA 
GATCCTGTCA CGTCTGTAGA CGTTGCACGG TTTGTTGGTA TCCACATCGC CATGGGAACT 
TTGAAGTTTC CCAGTCCAAG GCTTTACTGG GAGGACTTGA CGAAGGTGCC CCTGATTGCT 
GACGCCATGC CCCTGACTCG CTTTGTGGAG CTGTCCCGTA CGTTACAGCT ACCTTCTCCT 
GCTGAAGAGC AGCCACCTGG TGATGGACAG TCCAGAGATG ACGAGGGGAG GGACTTGAAC 
TGTTCACCAA CACAGATGGA TCCTCTGTGG AAGGTCCAGC CAATCCTGGG CCGCTTCAAT 
GCTGAGTGCC AGTCGTTGAG AAAGGAGGAC AACTATGCCG TTGACCAGTA TCCACTTCCT 
CTGACCTGCA AGACTCGCGA AAGTGGGCCG TCTCTGCTCT GCACTGCACT GATTGGACTT 
GGTGGTTTAC TCTTGCATGT GGATCTCAAT GTGGATCCCC CAGATAAAGA AGGTGTCGTA 
GAAAAGATGG CGCCTAAAAG TAGCATGGTG TTTCTTTGCA AACAGGAGCT TTCCACCCCT 
GCCATGATGG AACGCCTTTT AGATGCTGGG GTTCACGCGG CCGGTAAGGT GGGAGGAGCT 
CGAGGACAAA TCGGAGATGA GTTTGTGAGC TCCGACGGGA AGCTGATGCT CCGCAGATCC 
CACAGCGGCT TCATACTTTC TACAGCGGGG AACGGTCGGA GGAGCATGGC TTCCCTCACC 
AGCAGCTTCC AGAAGGCCCA GATGTCGGCT CGCCTCAACA GAGATTTGCT AAACCTGTAC 
TCCACACCTC TCACGGCCAC GCCTCCACAC AGCTGGCCCC AAGCGGTGCT CTGGTACCTC 
ATCGATTTGG CTTTGGTGAA CTCCTGGCTT TTGTACCGAG AAGATCACGG GGCAGCTGCT 
GGAGCTTTGA CTTTCATGGC TTTCAGGCTG GATGTTTCCA AGGCGCTGAC CCTCTCCGGC 
AGCTCAGACA CACAGGACTC CATTCCTCCT CAGCCTCCAT CAGAGGAAAC CCACGGGACA 
AGCCAAACGC CCAGTCCCAT GCTGGTGGAG GACAGCCCCC TGCCCGACGC CGCCACCCGC 
TACGACGGCG CAGGCCACTG GCCGGAGCAG CTGGCAGAGG GGGAAGGGGG CAGGTGTCGT 
TTCGGGGACT GTCAGAGGAC GTCGCGAGTT TTGTGTCTGA AGTGTTGCGT CTTTCTCTGC 
ATCTCACGAA ATCACAACTG CTTTTTGAAT TTTCATAACC AGGACAATAT AGGCAGAAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011471630.1
CDS	127..6429
Translation	MQSPSLTRTDSIPASSSASEQPVDMVAEPEQQGPEGQAGDHPAVAPADPQPPETSQHTQGGAQSPANVAAMPTAEAEDDAKRSTESSSGAKTNVQTVRRYVPSKKAMIDPLKMDMTKSLVEPLSSSQLSLHCVECHIIFSDHKSKDRHVKLKHPVEYEKRNLKNALFACYVCDRHFTNSTDLMAHQKAHTEKKPFKCPVCDQAFRRSSELTSHKKAHFGLFGYTCTECGKPCKTLTLLKYHQRTHTGERPYVCKECGKRFPMPKALHKHMISHSPEGAEGEEKDDAAKKRAKKDEGSPPVKLHCSMCKATFKNSKTRQRHMKIKHNVLPSGQSKPVITPISISQPTRLQLEAAGPLQKVDANIDTAQIRKLIESLGTVKKVNQVVLLGQVPGYAPPLELQADSQPAGPLDPDLSPPQIDFGGLKPPESKTTGLDTSNYPCGFMDQTIILEPITPDGETPPLSELASCVAAGETAQTVGEVVQRPLTSEILLDNMTCQNEENDLEQTFILELTPALTPTAELEHSQTEPQNMAPSSSLVLNTDQEAADQTAKKSALNGQDASLTEPPLVSTAQLDGTSPSLDPEHKELPSNPLDSPDKPSQVGPNEGDDACTKMTGQDQLSEGELKTQEHNGEHLSEKSGMEGKKNHSEVESSSKAAVPSDMQSKHVPQTSELALNVMSAQELVKVRKRKPARTFFFPGYIQDLAGSIYKDDFLIDAKPAKRQRTKKSHLVVKFSPQSKDKKNKKKKQEPQQPRPEDLTKGKAPAKRTPEKKMSLSPKKGAKGKKDAAGGGGASGVVTKSPPTPVAQEKQIKEDVGKNKLKKKKVGNEDAQTSGQKAAASPVFKKKKQPKVRQEVSPKSAKTERARKKTPTKKVKKPEEPNLNAVQDSLLLLKGHKQPQLKVYKLDPSRASSQPQDASVSKKKGGRAKKALSLMSSLKSSQPPPDAPPNKPKATRKRKASSKVETEGVITSKRALECKDCGEKFSDVSSLQKHKTTAHTVESPSLTYTNGNIFEGVSRLEFYPSPKVYDGGAAGKNAATGWDTEPEMGEMTVEDREQNVSFPALIPSPSLPVSPPNVDINAFQGASRRDENKASPLDVCSRPRDMTAGKDRTDFLESAQAKSSERRPLFPPECASKSPNSECEAQGTTEEDFKEDLLLEVDLVTVGEQNERDRSSLDEDTSTQKDLNTSDSESDKHPEQICDGRSENEVTSKTVFCSTNQVKEEEEEMLVQRRGEDQKSVVMSRRRRRGSEPRRGDAIRKTGSVGESQTETESEQDECQVVYEIHSVFSDQESDTKTSPGFRTMKGPASVAFIPSGPSAFDKSSEERVVLELESVTTSVQEVMNEHRLHGDGETDREADQSPGIVLERFLTSRQRSSDKEASCRAADQREGFDGVSEKEVQFVGSQEIKVEETPPDPTLQPPACQSRQRAGLHPQHNRAIRTVLVKEESGPLLNAALDAQGSRRMQWAVEPISSGGADGPLIENGQSPNEARRSPEFNTKQCIFYPVKEEERELLLASAQTNADTFSLEGFGDGAPAEHQPAECDAYGGRLSAAGYQNGSLLSEPETSDFTDSQAFSDSEWQPSDVRDFLLKSCDDDVSGFELPDPQRDTEAEVMAYFNRKQSSSSHEPNHTSASFSTSSHLQPQMNGTRTREPIDYFSKYFGWDTWAETSTCTNKLCKMSDPVTSVDVARFVGIHIAMGTLKFPSPRLYWEDLTKVPLIADAMPLTRFVELSRTLQLPSPAEEQPPGDGQSRDDEGRDLNCSPTQMDPLWKVQPILGRFNAECQSLRKEDNYAVDQYPLPLTCKTRESGPSLLCTALIGLGGLLLHVDLNVDPPDKEGVVEKMAPKSSMVFLCKQELSTPAMMERLLDAGVHAAGKVGGARGQIGDEFVSSDGKLMLRRSHSGFILSTAGNGRRSMASLTSSFQKAQMSARLNRDLLNLYSTPLTATPPHSWPQAVLWYLIDLALVNSWLLYREDHGAAAGALTFMAFRLDVSKALTLSGSSDTQDSIPPQPPSEETHGTSQTPSPMLVEDSPLPDAATRYDGAGHWPEQLAEGEGGRCRFGDCQRTSRVLCLKCCVFLCISRNHNCFLNFHNQDNIGRK

Target ORF information:

RefSeq Version	XM_011473328.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes uncharacterized LOC101162913 (LOC101162913), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011473328.3

ORF Insert Sequence:

ATGCAGAGTC CATCTTTGAC CCGGACGGAC TCCATCCCTG CCTCCTCATC GGCCTCAGAG 
CAGCCCGTGG ACATGGTGGC TGAGCCGGAG CAGCAGGGAC CAGAGGGACA GGCGGGGGAC 
CACCCGGCAG TGGCTCCTGC AGACCCCCAA CCACCAGAGA CGAGTCAACA CACCCAAGGG 
GGAGCACAGA GCCCTGCAAA TGTAGCGGCC ATGCCAACAG CAGAGGCTGA AGATGATGCC 
AAGAGAAGCA CTGAGAGCAG CAGTGGAGCG AAGACAAACG TGCAGACCGT CAGGAGATAC 
GTCCCTTCCA AGAAAGCAAT GATAGATCCT CTGAAGATGG ATATGACCAA ATCGCTGGTT 
GAGCCACTCT CATCCTCTCA GCTCTCTCTG CACTGCGTCG AGTGCCACAT CATCTTCAGT 
GACCACAAGA GCAAAGACCG CCACGTGAAG CTGAAACACC CAGTAGAATA TGAAAAGCGC 
AACCTGAAAA ACGCCCTTTT TGCATGTTAC GTGTGTGACC GCCATTTTAC CAACTCCACG 
GACCTCATGG CCCACCAGAA GGCCCACACT GAGAAGAAAC CCTTCAAGTG TCCCGTGTGC 
GATCAGGCCT TCAGGAGGTC CTCCGAGCTC ACCAGTCATA AAAAAGCTCA TTTCGGCCTG 
TTCGGCTACA CCTGCACGGA GTGCGGCAAA CCCTGCAAAA CGCTGACATT GCTGAAGTAT 
CACCAGCGTA CGCACACAGG AGAGAGGCCG TACGTCTGCA AGGAGTGTGG GAAGAGGTTC 
CCCATGCCGA AAGCTTTGCA CAAACACATG ATCTCCCACT CTCCAGAGGG AGCTGAAGGA 
GAAGAAAAAG ACGACGCTGC CAAAAAACGA GCAAAGAAAG ATGAAGGTTC TCCTCCTGTC 
AAACTCCACT GCTCCATGTG CAAGGCCACT TTCAAGAACT CCAAGACTCG CCAGCGGCAC 
ATGAAAATCA AGCACAACGT CCTCCCATCA GGGCAAAGCA AACCTGTTAT CACCCCCATT 
TCGATCTCAC AGCCCACGCG GTTGCAGCTA GAGGCCGCTG GACCTCTGCA AAAAGTTGAT 
GCCAACATCG ACACAGCGCA AATACGCAAA CTTATAGAGT CGCTGGGCAC TGTGAAGAAA 
GTGAACCAAG TGGTTCTGTT GGGTCAGGTG CCGGGTTACG CTCCACCACT GGAATTGCAG 
GCGGATTCGC AGCCAGCAGG GCCTCTGGAT CCCGATCTAA GTCCACCGCA GATCGATTTT 
GGAGGACTGA AGCCGCCCGA ATCCAAGACG ACTGGACTGG ACACTTCCAA TTATCCGTGT 
GGGTTCATGG ACCAAACCAT CATTCTGGAA CCCATCACTC CTGATGGAGA AACCCCCCCC 
CTGTCAGAGC TGGCATCCTG TGTAGCAGCA GGCGAAACTG CACAGACTGT GGGAGAGGTG 
GTTCAGAGGC CCCTGACCAG TGAAATTCTC TTGGATAATA TGACCTGCCA GAACGAGGAG 
AATGACCTTG AACAAACATT CATATTAGAA CTCACCCCAG CTTTGACACC AACTGCAGAA 
CTGGAGCACT CCCAGACTGA ACCACAGAAT ATGGCTCCGT CCTCCTCTCT GGTGCTGAAC 
ACGGACCAGG AGGCTGCAGA TCAAACTGCC AAAAAGTCTG CCCTCAATGG GCAAGACGCC 
AGCCTGACAG AACCGCCACT TGTCTCCACA GCCCAGCTGG ATGGAACATC TCCTTCCTTA 
GACCCAGAGC ACAAAGAACT TCCCTCCAAC CCTTTGGATT CACCAGATAA ACCCTCACAA 
GTTGGCCCCA ATGAAGGCGA TGATGCATGT ACGAAGATGA CGGGTCAAGA CCAGTTGAGT 
GAGGGCGAAT TAAAGACACA GGAACACAAT GGAGAGCATT TGTCTGAGAA ATCTGGAATG 
GAAGGTAAGA AGAACCATTC AGAAGTGGAG TCGTCTTCTA AAGCGGCAGT CCCGTCTGAC 
ATGCAGTCAA AACATGTTCC TCAGACCTCA GAACTGGCCC TGAATGTCAT GTCTGCTCAG 
GAGCTGGTGA AAGTGCGCAA AAGAAAGCCG GCCAGGACGT TTTTCTTTCC AGGATACATT 
CAAGATCTGG CCGGGTCCAT TTACAAAGAC GACTTTCTGA TCGACGCCAA ACCAGCCAAG 
CGGCAAAGAA CAAAAAAGTC TCATCTTGTT GTGAAATTCA GTCCTCAGAG CAAAGACAAG 
AAAAATAAGA AAAAAAAGCA GGAACCGCAG CAGCCAAGAC CAGAAGATCT GACTAAAGGT 
AAAGCTCCGG CTAAGAGAAC ACCTGAGAAA AAGATGTCCT TGTCACCAAA GAAAGGAGCA 
AAGGGAAAAA AGGATGCTGC AGGGGGGGGC GGAGCATCAG GAGTTGTCAC AAAGTCCCCT 
CCAACCCCAG TTGCACAGGA GAAACAAATC AAAGAAGATG TGGGTAAAAA CAAACTAAAA 
AAAAAAAAAG TAGGGAACGA AGATGCACAA ACGAGTGGGC AGAAGGCCGC AGCTTCACCT 
GTGTTCAAAA AGAAAAAGCA ACCAAAGGTT AGACAAGAAG TTTCACCCAA AAGTGCAAAA 
ACTGAAAGAG CTAGGAAAAA AACACCAACC AAAAAAGTCA AAAAGCCAGA AGAACCGAAT 
TTAAACGCAG TACAAGACTC TCTGCTTTTA TTAAAAGGCC ATAAGCAGCC CCAGCTCAAA 
GTTTACAAGC TGGACCCTTC CAGGGCGTCG AGTCAGCCTC AGGACGCCTC CGTCTCCAAG 
AAAAAGGGAG GACGGGCAAA GAAAGCTCTG TCCCTCATGT CTTCCTTGAA GTCTTCTCAA 
CCTCCACCCG ATGCTCCGCC CAACAAGCCA AAGGCCACCA GAAAACGCAA AGCCTCGTCG 
AAAGTGGAAA CTGAAGGAGT CATAACCTCT AAGCGAGCCT TGGAGTGCAA AGACTGTGGG 
GAAAAGTTCA GTGACGTCTC ATCGCTTCAG AAGCACAAAA CGACCGCACA CACCGTAGAG 
AGTCCAAGTC TCACCTACAC CAACGGAAAC ATATTTGAGG GCGTCTCCAG GTTGGAGTTT 
TACCCGTCCC CGAAGGTGTA TGACGGCGGA GCCGCGGGAA AGAATGCAGC AACAGGGTGG 
GACACTGAGC CAGAGATGGG AGAGATGACT GTGGAGGACA GGGAGCAGAA TGTCTCTTTC 
CCAGCTCTGA TTCCATCCCC ATCTCTGCCG GTTTCTCCTC CAAATGTTGA CATAAATGCC 
TTCCAGGGGG CAAGTAGAAG AGACGAGAAC AAAGCCTCCC CTTTAGATGT TTGCTCTCGG 
CCCCGTGACA TGACTGCTGG CAAGGATCGC ACAGATTTCC TAGAGTCCGC TCAGGCGAAG 
TCCTCTGAGA GGAGGCCGCT GTTTCCACCA GAATGTGCCT CAAAGAGCCC CAACTCTGAA 
TGTGAAGCCC AGGGAACCAC CGAGGAAGAC TTCAAGGAAG ATCTACTTCT AGAGGTTGAC 
TTGGTTACTG TTGGGGAACA GAATGAGAGA GACAGAAGTT CTCTGGATGA AGACACTTCT 
ACCCAAAAAG ATTTAAACAC CAGTGATTCT GAAAGTGATA AACACCCCGA GCAGATTTGT 
GATGGAAGGA GTGAAAATGA GGTAACTTCA AAGACTGTGT TTTGCTCAAC CAATCAGGTG 
AAAGAAGAAG AGGAAGAGAT GTTGGTGCAG AGGAGAGGAG AGGACCAGAA GAGCGTTGTG 
ATGAGTAGAC GTAGGAGAAG AGGCTCAGAG CCCAGAAGAG GAGATGCCAT AAGAAAGACG 
GGATCTGTTG GAGAGTCTCA GACGGAAACT GAAAGTGAGC AGGATGAATG TCAGGTGGTT 
TATGAGATAC ACTCCGTCTT CTCGGACCAG GAGTCTGACA CGAAGACCAG CCCTGGATTC 
AGGACCATGA AAGGCCCTGC TTCTGTTGCG TTCATCCCTT CTGGTCCTTC AGCGTTTGAC 
AAATCCTCTG AAGAGCGTGT AGTGCTGGAG CTGGAGTCTG TAACCACTAG TGTTCAGGAA 
GTGATGAACG AACACAGGCT GCATGGAGAC GGGGAGACGG ACAGGGAAGC AGACCAGTCT 
CCAGGCATCG TGCTGGAGAG GTTCCTGACC TCCAGACAAA GATCTTCTGA CAAAGAGGCC 
TCCTGCAGGG CTGCAGACCA ACGAGAGGGT TTTGATGGTG TTTCTGAGAA AGAAGTACAG 
TTTGTTGGGA GCCAGGAGAT CAAAGTTGAA GAGACCCCGC CGGACCCCAC ACTGCAGCCC 
CCTGCCTGTC AAAGCAGGCA GAGGGCCGGT CTCCATCCAC AGCATAATCG AGCCATACGG 
ACCGTTCTAG TGAAAGAGGA GAGCGGCCCC CTGCTGAACG CTGCTCTGGA CGCCCAAGGC 
AGCAGACGGA TGCAGTGGGC AGTGGAGCCA ATCAGCAGCG GGGGGGCCGA TGGGCCGTTA 
ATTGAGAATG GGCAGAGCCC CAATGAGGCA CGCAGGAGTC CAGAGTTCAA CACCAAGCAG 
TGCATCTTCT ATCCAGTGAA GGAGGAGGAG CGGGAACTTC TGCTGGCATC TGCTCAGACC 
AACGCTGACA CCTTCAGCCT GGAAGGCTTC GGTGATGGAG CCCCAGCTGA GCATCAACCT 
GCAGAGTGTG ATGCATATGG AGGAAGACTC TCTGCTGCAG GCTACCAGAA TGGAAGCCTG 
CTGTCAGAAC CAGAGACTTC AGACTTCACT GACAGTCAAG CTTTCTCTGA CTCGGAGTGG 
CAGCCGTCAG ATGTGCGGGA CTTTCTACTC AAGAGTTGTG ATGACGACGT GAGCGGCTTC 
GAACTGCCTG ATCCTCAGCG TGACACAGAA GCAGAAGTGA TGGCTTACTT CAACAGGAAG 
CAGTCCAGCA GCTCGCATGA GCCCAATCAC ACATCCGCGA GTTTTTCAAC ATCGAGTCAT 
CTGCAACCCC AGATGAATGG AACCAGGACC AGAGAGCCCA TTGATTACTT TTCCAAATAT 
TTTGGTTGGG ATACTTGGGC AGAAACTTCA ACTTGCACAA ACAAACTCTG CAAAATGTCA 
GATCCTGTCA CGTCTGTAGA CGTTGCACGG TTTGTTGGTA TCCACATCGC CATGGGAACT 
TTGAAGTTTC CCAGTCCAAG GCTTTACTGG GAGGACTTGA CGAAGGTGCC CCTGATTGCT 
GACGCCATGC CCCTGACTCG CTTTGTGGAG CTGTCCCGTA CGTTACAGCT ACCTTCTCCT 
GCTGAAGAGC AGCCACCTGG TGATGGACAG TCCAGAGATG ACGAGGGGAG GGACTTGAAC 
TGTTCACCAA CACAGATGGA TCCTCTGTGG AAGGTCCAGC CAATCCTGGG CCGCTTCAAT 
GCTGAGTGCC AGTCGTTGAG AAAGGAGGAC AACTATGCCG TTGACCAGTA TCCACTTCCT 
CTGACCTGCA AGACTCGCGA AAGTGGGCCG TCTCTGCTCT GCACTGCACT GATTGGACTT 
GGTGGTTTAC TCTTGCATGT GGATCTCAAT GTGGATCCCC CAGATAAAGA AGGTGTCGTA 
GAAAAGATGG CGCCTAAAAG TAGCATGGTG TTTCTTTGCA AACAGGAGCT TTCCACCCCT 
GCCATGATGG AACGCCTTTT AGATGCTGGG GTTCACGCGG CCGGTAAGGT GGGAGGAGCT 
CGAGGACAAA TCGGAGATGA GTTTGTGAGC TCCGACGGGA AGCTGATGCT CCGCAGATCC 
CACAGCGGCT TCATACTTTC TACAGCGGGG AACGGTCGGA GGAGCATGGC TTCCCTCACC 
AGCAGCTTCC AGAAGGCCCA GATGTCGGCT CGCCTCAACA GAGATTTGCT AAACCTGTAC 
TCCACACCTC TCACGGCCAC GCCTCCACAC AGCTGGCCCC AAGCGGTGCT CTGGTACCTC 
ATCGATTTGG CTTTGGTGAA CTCCTGGCTT TTGTACCGAG AAGATCACGG GGCAGCTGCT 
GGAGCTTTGA CTTTCATGGC TTTCAGGCTG GATGTTTCCA AGGCGCTGAC CCTCTCCGGC 
AGCTCAGACA CACAGGACTC CATTCCTCCT CAGCCTCCAT CAGAGGAAAC CCACGGGACA 
AGCCAAACGC CCAGTCCCAT GCTGGTGGAG GACAGCCCCC TGCCCGACGC CGCCACCCGC 
TACGACGGCG CAGGCCACTG GCCGGAGCAG CTGGCAGAGG GGGAAGGGGG CAGGTGTCGT 
TTCGGGGACT GTCAGAGGAC GTCGCGAGTT TTGTGTCTGA AGTGTTGCGT CTTTCTCTGC 
ATCTCACGAA ATCACAACTG CTTTTTGAAT TTTCATAACC AGGACAATAT AGGCAGAAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101162913 ( XM_011473328.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011471630.1 Oryzias latipes uncharacterized LOC101162913 (LOC101162913), transcript variant X1, mRNA.

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CloneID	OJa27422
Clone ID Related Accession (Same CDS sequence)	XM_011473328.3 , XM_011473328.2 , XM_020701850.1 , XM_020701850.2
Accession Version	XM_011473328.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	uncharacterized protein LOC101162913
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004095190.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011473328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGTC CATCTTTGAC CCGGACGGAC TCCATCCCTG CCTCCTCATC GGCCTCAGAG 
CAGCCCGTGG ACATGGTGGC TGAGCCGGAG CAGCAGGGAC CAGAGGGACA GGCGGGGGAC 
CACCCGGCAG TGGCTCCTGC AGACCCCCAA CCACCAGAGA CGAGTCAACA CACCCAAGGG 
GGAGCACAGA GCCCTGCAAA TGTAGCGGCC ATGCCAACAG CAGAGGCTGA AGATGATGCC 
AAGAGAAGCA CTGAGAGCAG CAGTGGAGCG AAGACAAACG TGCAGACCGT CAGGAGATAC 
GTCCCTTCCA AGAAAGCAAT GATAGATCCT CTGAAGATGG ATATGACCAA ATCGCTGGTT 
GAGCCACTCT CATCCTCTCA GCTCTCTCTG CACTGCGTCG AGTGCCACAT CATCTTCAGT 
GACCACAAGA GCAAAGACCG CCACGTGAAG CTGAAACACC CAGTAGAATA TGAAAAGCGC 
AACCTGAAAA ACGCCCTTTT TGCATGTTAC GTGTGTGACC GCCATTTTAC CAACTCCACG 
GACCTCATGG CCCACCAGAA GGCCCACACT GAGAAGAAAC CCTTCAAGTG TCCCGTGTGC 
GATCAGGCCT TCAGGAGGTC CTCCGAGCTC ACCAGTCATA AAAAAGCTCA TTTCGGCCTG 
TTCGGCTACA CCTGCACGGA GTGCGGCAAA CCCTGCAAAA CGCTGACATT GCTGAAGTAT 
CACCAGCGTA CGCACACAGG AGAGAGGCCG TACGTCTGCA AGGAGTGTGG GAAGAGGTTC 
CCCATGCCGA AAGCTTTGCA CAAACACATG ATCTCCCACT CTCCAGAGGG AGCTGAAGGA 
GAAGAAAAAG ACGACGCTGC CAAAAAACGA GCAAAGAAAG ATGAAGGTTC TCCTCCTGTC 
AAACTCCACT GCTCCATGTG CAAGGCCACT TTCAAGAACT CCAAGACTCG CCAGCGGCAC 
ATGAAAATCA AGCACAACGT CCTCCCATCA GGGCAAAGCA AACCTGTTAT CACCCCCATT 
TCGATCTCAC AGCCCACGCG GTTGCAGCTA GAGGCCGCTG GACCTCTGCA AAAAGTTGAT 
GCCAACATCG ACACAGCGCA AATACGCAAA CTTATAGAGT CGCTGGGCAC TGTGAAGAAA 
GTGAACCAAG TGGTTCTGTT GGGTCAGGTG CCGGGTTACG CTCCACCACT GGAATTGCAG 
GCGGATTCGC AGCCAGCAGG GCCTCTGGAT CCCGATCTAA GTCCACCGCA GATCGATTTT 
GGAGGACTGA AGCCGCCCGA ATCCAAGACG ACTGGACTGG ACACTTCCAA TTATCCGTGT 
GGGTTCATGG ACCAAACCAT CATTCTGGAA CCCATCACTC CTGATGGAGA AACCCCCCCC 
CTGTCAGAGC TGGCATCCTG TGTAGCAGCA GGCGAAACTG CACAGACTGT GGGAGAGGTG 
GTTCAGAGGC CCCTGACCAG TGAAATTCTC TTGGATAATA TGACCTGCCA GAACGAGGAG 
AATGACCTTG AACAAACATT CATATTAGAA CTCACCCCAG CTTTGACACC AACTGCAGAA 
CTGGAGCACT CCCAGACTGA ACCACAGAAT ATGGCTCCGT CCTCCTCTCT GGTGCTGAAC 
ACGGACCAGG AGGCTGCAGA TCAAACTGCC AAAAAGTCTG CCCTCAATGG GCAAGACGCC 
AGCCTGACAG AACCGCCACT TGTCTCCACA GCCCAGCTGG ATGGAACATC TCCTTCCTTA 
GACCCAGAGC ACAAAGAACT TCCCTCCAAC CCTTTGGATT CACCAGATAA ACCCTCACAA 
GTTGGCCCCA ATGAAGGCGA TGATGCATGT ACGAAGATGA CGGGTCAAGA CCAGTTGAGT 
GAGGGCGAAT TAAAGACACA GGAACACAAT GGAGAGCATT TGTCTGAGAA ATCTGGAATG 
GAAGGTAAGA AGAACCATTC AGAAGTGGAG TCGTCTTCTA AAGCGGCAGT CCCGTCTGAC 
ATGCAGTCAA AACATGTTCC TCAGACCTCA GAACTGGCCC TGAATGTCAT GTCTGCTCAG 
GAGCTGGTGA AAGTGCGCAA AAGAAAGCCG GCCAGGACGT TTTTCTTTCC AGGATACATT 
CAAGATCTGG CCGGGTCCAT TTACAAAGAC GACTTTCTGA TCGACGCCAA ACCAGCCAAG 
CGGCAAAGAA CAAAAAAGTC TCATCTTGTT GTGAAATTCA GTCCTCAGAG CAAAGACAAG 
AAAAATAAGA AAAAAAAGCA GGAACCGCAG CAGCCAAGAC CAGAAGATCT GACTAAAGGT 
AAAGCTCCGG CTAAGAGAAC ACCTGAGAAA AAGATGTCCT TGTCACCAAA GAAAGGAGCA 
AAGGGAAAAA AGGATGCTGC AGGGGGGGGC GGAGCATCAG GAGTTGTCAC AAAGTCCCCT 
CCAACCCCAG TTGCACAGGA GAAACAAATC AAAGAAGATG TGGGTAAAAA CAAACTAAAA 
AAAAAAAAAG TAGGGAACGA AGATGCACAA ACGAGTGGGC AGAAGGCCGC AGCTTCACCT 
GTGTTCAAAA AGAAAAAGCA ACCAAAGGTT AGACAAGAAG TTTCACCCAA AAGTGCAAAA 
ACTGAAAGAG CTAGGAAAAA AACACCAACC AAAAAAGTCA AAAAGCCAGA AGAACCGAAT 
TTAAACGCAG TACAAGACTC TCTGCTTTTA TTAAAAGGCC ATAAGCAGCC CCAGCTCAAA 
GTTTACAAGC TGGACCCTTC CAGGGCGTCG AGTCAGCCTC AGGACGCCTC CGTCTCCAAG 
AAAAAGGGAG GACGGGCAAA GAAAGCTCTG TCCCTCATGT CTTCCTTGAA GTCTTCTCAA 
CCTCCACCCG ATGCTCCGCC CAACAAGCCA AAGGCCACCA GAAAACGCAA AGCCTCGTCG 
AAAGTGGAAA CTGAAGGAGT CATAACCTCT AAGCGAGCCT TGGAGTGCAA AGACTGTGGG 
GAAAAGTTCA GTGACGTCTC ATCGCTTCAG AAGCACAAAA CGACCGCACA CACCGTAGAG 
AGTCCAAGTC TCACCTACAC CAACGGAAAC ATATTTGAGG GCGTCTCCAG GTTGGAGTTT 
TACCCGTCCC CGAAGGTGTA TGACGGCGGA GCCGCGGGAA AGAATGCAGC AACAGGGTGG 
GACACTGAGC CAGAGATGGG AGAGATGACT GTGGAGGACA GGGAGCAGAA TGTCTCTTTC 
CCAGCTCTGA TTCCATCCCC ATCTCTGCCG GTTTCTCCTC CAAATGTTGA CATAAATGCC 
TTCCAGGGGG CAAGTAGAAG AGACGAGAAC AAAGCCTCCC CTTTAGATGT TTGCTCTCGG 
CCCCGTGACA TGACTGCTGG CAAGGATCGC ACAGATTTCC TAGAGTCCGC TCAGGCGAAG 
TCCTCTGAGA GGAGGCCGCT GTTTCCACCA GAATGTGCCT CAAAGAGCCC CAACTCTGAA 
TGTGAAGCCC AGGGAACCAC CGAGGAAGAC TTCAAGGAAG ATCTACTTCT AGAGGTTGAC 
TTGGTTACTG TTGGGGAACA GAATGAGAGA GACAGAAGTT CTCTGGATGA AGACACTTCT 
ACCCAAAAAG ATTTAAACAC CAGTGATTCT GAAAGTGATA AACACCCCGA GCAGATTTGT 
GATGGAAGGA GTGAAAATGA GGTAACTTCA AAGACTGTGT TTTGCTCAAC CAATCAGGTG 
AAAGAAGAAG AGGAAGAGAT GTTGGTGCAG AGGAGAGGAG AGGACCAGAA GAGCGTTGTG 
ATGAGTAGAC GTAGGAGAAG AGGCTCAGAG CCCAGAAGAG GAGATGCCAT AAGAAAGACG 
GGATCTGTTG GAGAGTCTCA GACGGAAACT GAAAGTGAGC AGGATGAATG TCAGGTGGTT 
TATGAGATAC ACTCCGTCTT CTCGGACCAG GAGTCTGACA CGAAGACCAG CCCTGGATTC 
AGGACCATGA AAGGCCCTGC TTCTGTTGCG TTCATCCCTT CTGGTCCTTC AGCGTTTGAC 
AAATCCTCTG AAGAGCGTGT AGTGCTGGAG CTGGAGTCTG TAACCACTAG TGTTCAGGAA 
GTGATGAACG AACACAGGCT GCATGGAGAC GGGGAGACGG ACAGGGAAGC AGACCAGTCT 
CCAGGCATCG TGCTGGAGAG GTTCCTGACC TCCAGACAAA GATCTTCTGA CAAAGAGGCC 
TCCTGCAGGG CTGCAGACCA ACGAGAGGGT TTTGATGGTG TTTCTGAGAA AGAAGTACAG 
TTTGTTGGGA GCCAGGAGAT CAAAGTTGAA GAGACCCCGC CGGACCCCAC ACTGCAGCCC 
CCTGCCTGTC AAAGCAGGCA GAGGGCCGGT CTCCATCCAC AGCATAATCG AGCCATACGG 
ACCGTTCTAG TGAAAGAGGA GAGCGGCCCC CTGCTGAACG CTGCTCTGGA CGCCCAAGGC 
AGCAGACGGA TGCAGTGGGC AGTGGAGCCA ATCAGCAGCG GGGGGGCCGA TGGGCCGTTA 
ATTGAGAATG GGCAGAGCCC CAATGAGGCA CGCAGGAGTC CAGAGTTCAA CACCAAGCAG 
TGCATCTTCT ATCCAGTGAA GGAGGAGGAG CGGGAACTTC TGCTGGCATC TGCTCAGACC 
AACGCTGACA CCTTCAGCCT GGAAGGCTTC GGTGATGGAG CCCCAGCTGA GCATCAACCT 
GCAGAGTGTG ATGCATATGG AGGAAGACTC TCTGCTGCAG GCTACCAGAA TGGAAGCCTG 
CTGTCAGAAC CAGAGACTTC AGACTTCACT GACAGTCAAG CTTTCTCTGA CTCGGAGTGG 
CAGCCGTCAG ATGTGCGGGA CTTTCTACTC AAGAGTTGTG ATGACGACGT GAGCGGCTTC 
GAACTGCCTG ATCCTCAGCG TGACACAGAA GCAGAAGTGA TGGCTTACTT CAACAGGAAG 
CAGTCCAGCA GCTCGCATGA GCCCAATCAC ACATCCGCGA GTTTTTCAAC ATCGAGTCAT 
CTGCAACCCC AGATGAATGG AACCAGGACC AGAGAGCCCA TTGATTACTT TTCCAAATAT 
TTTGGTTGGG ATACTTGGGC AGAAACTTCA ACTTGCACAA ACAAACTCTG CAAAATGTCA 
GATCCTGTCA CGTCTGTAGA CGTTGCACGG TTTGTTGGTA TCCACATCGC CATGGGAACT 
TTGAAGTTTC CCAGTCCAAG GCTTTACTGG GAGGACTTGA CGAAGGTGCC CCTGATTGCT 
GACGCCATGC CCCTGACTCG CTTTGTGGAG CTGTCCCGTA CGTTACAGCT ACCTTCTCCT 
GCTGAAGAGC AGCCACCTGG TGATGGACAG TCCAGAGATG ACGAGGGGAG GGACTTGAAC 
TGTTCACCAA CACAGATGGA TCCTCTGTGG AAGGTCCAGC CAATCCTGGG CCGCTTCAAT 
GCTGAGTGCC AGTCGTTGAG AAAGGAGGAC AACTATGCCG TTGACCAGTA TCCACTTCCT 
CTGACCTGCA AGACTCGCGA AAGTGGGCCG TCTCTGCTCT GCACTGCACT GATTGGACTT 
GGTGGTTTAC TCTTGCATGT GGATCTCAAT GTGGATCCCC CAGATAAAGA AGGTGTCGTA 
GAAAAGATGG CGCCTAAAAG TAGCATGGTG TTTCTTTGCA AACAGGAGCT TTCCACCCCT 
GCCATGATGG AACGCCTTTT AGATGCTGGG GTTCACGCGG CCGGTAAGGT GGGAGGAGCT 
CGAGGACAAA TCGGAGATGA GTTTGTGAGC TCCGACGGGA AGCTGATGCT CCGCAGATCC 
CACAGCGGCT TCATACTTTC TACAGCGGGG AACGGTCGGA GGAGCATGGC TTCCCTCACC 
AGCAGCTTCC AGAAGGCCCA GATGTCGGCT CGCCTCAACA GAGATTTGCT AAACCTGTAC 
TCCACACCTC TCACGGCCAC GCCTCCACAC AGCTGGCCCC AAGCGGTGCT CTGGTACCTC 
ATCGATTTGG CTTTGGTGAA CTCCTGGCTT TTGTACCGAG AAGATCACGG GGCAGCTGCT 
GGAGCTTTGA CTTTCATGGC TTTCAGGCTG GATGTTTCCA AGGCGCTGAC CCTCTCCGGC 
AGCTCAGACA CACAGGACTC CATTCCTCCT CAGCCTCCAT CAGAGGAAAC CCACGGGACA 
AGCCAAACGC CCAGTCCCAT GCTGGTGGAG GACAGCCCCC TGCCCGACGC CGCCACCCGC 
TACGACGGCG CAGGCCACTG GCCGGAGCAG CTGGCAGAGG GGGAAGGGGG CAGGTGTCGT 
TTCGGGGACT GTCAGAGGAC GTCGCGAGTT TTGTGTCTGA AGTGTTGCGT CTTTCTCTGC 
ATCTCACGAA ATCACAACTG CTTTTTGAAT TTTCATAACC AGGACAATAT AGGCAGAAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011471630.1
CDS	128..6430
Translation	MQSPSLTRTDSIPASSSASEQPVDMVAEPEQQGPEGQAGDHPAVAPADPQPPETSQHTQGGAQSPANVAAMPTAEAEDDAKRSTESSSGAKTNVQTVRRYVPSKKAMIDPLKMDMTKSLVEPLSSSQLSLHCVECHIIFSDHKSKDRHVKLKHPVEYEKRNLKNALFACYVCDRHFTNSTDLMAHQKAHTEKKPFKCPVCDQAFRRSSELTSHKKAHFGLFGYTCTECGKPCKTLTLLKYHQRTHTGERPYVCKECGKRFPMPKALHKHMISHSPEGAEGEEKDDAAKKRAKKDEGSPPVKLHCSMCKATFKNSKTRQRHMKIKHNVLPSGQSKPVITPISISQPTRLQLEAAGPLQKVDANIDTAQIRKLIESLGTVKKVNQVVLLGQVPGYAPPLELQADSQPAGPLDPDLSPPQIDFGGLKPPESKTTGLDTSNYPCGFMDQTIILEPITPDGETPPLSELASCVAAGETAQTVGEVVQRPLTSEILLDNMTCQNEENDLEQTFILELTPALTPTAELEHSQTEPQNMAPSSSLVLNTDQEAADQTAKKSALNGQDASLTEPPLVSTAQLDGTSPSLDPEHKELPSNPLDSPDKPSQVGPNEGDDACTKMTGQDQLSEGELKTQEHNGEHLSEKSGMEGKKNHSEVESSSKAAVPSDMQSKHVPQTSELALNVMSAQELVKVRKRKPARTFFFPGYIQDLAGSIYKDDFLIDAKPAKRQRTKKSHLVVKFSPQSKDKKNKKKKQEPQQPRPEDLTKGKAPAKRTPEKKMSLSPKKGAKGKKDAAGGGGASGVVTKSPPTPVAQEKQIKEDVGKNKLKKKKVGNEDAQTSGQKAAASPVFKKKKQPKVRQEVSPKSAKTERARKKTPTKKVKKPEEPNLNAVQDSLLLLKGHKQPQLKVYKLDPSRASSQPQDASVSKKKGGRAKKALSLMSSLKSSQPPPDAPPNKPKATRKRKASSKVETEGVITSKRALECKDCGEKFSDVSSLQKHKTTAHTVESPSLTYTNGNIFEGVSRLEFYPSPKVYDGGAAGKNAATGWDTEPEMGEMTVEDREQNVSFPALIPSPSLPVSPPNVDINAFQGASRRDENKASPLDVCSRPRDMTAGKDRTDFLESAQAKSSERRPLFPPECASKSPNSECEAQGTTEEDFKEDLLLEVDLVTVGEQNERDRSSLDEDTSTQKDLNTSDSESDKHPEQICDGRSENEVTSKTVFCSTNQVKEEEEEMLVQRRGEDQKSVVMSRRRRRGSEPRRGDAIRKTGSVGESQTETESEQDECQVVYEIHSVFSDQESDTKTSPGFRTMKGPASVAFIPSGPSAFDKSSEERVVLELESVTTSVQEVMNEHRLHGDGETDREADQSPGIVLERFLTSRQRSSDKEASCRAADQREGFDGVSEKEVQFVGSQEIKVEETPPDPTLQPPACQSRQRAGLHPQHNRAIRTVLVKEESGPLLNAALDAQGSRRMQWAVEPISSGGADGPLIENGQSPNEARRSPEFNTKQCIFYPVKEEERELLLASAQTNADTFSLEGFGDGAPAEHQPAECDAYGGRLSAAGYQNGSLLSEPETSDFTDSQAFSDSEWQPSDVRDFLLKSCDDDVSGFELPDPQRDTEAEVMAYFNRKQSSSSHEPNHTSASFSTSSHLQPQMNGTRTREPIDYFSKYFGWDTWAETSTCTNKLCKMSDPVTSVDVARFVGIHIAMGTLKFPSPRLYWEDLTKVPLIADAMPLTRFVELSRTLQLPSPAEEQPPGDGQSRDDEGRDLNCSPTQMDPLWKVQPILGRFNAECQSLRKEDNYAVDQYPLPLTCKTRESGPSLLCTALIGLGGLLLHVDLNVDPPDKEGVVEKMAPKSSMVFLCKQELSTPAMMERLLDAGVHAAGKVGGARGQIGDEFVSSDGKLMLRRSHSGFILSTAGNGRRSMASLTSSFQKAQMSARLNRDLLNLYSTPLTATPPHSWPQAVLWYLIDLALVNSWLLYREDHGAAAGALTFMAFRLDVSKALTLSGSSDTQDSIPPQPPSEETHGTSQTPSPMLVEDSPLPDAATRYDGAGHWPEQLAEGEGGRCRFGDCQRTSRVLCLKCCVFLCISRNHNCFLNFHNQDNIGRK

Target ORF information:

RefSeq Version	XM_011473328.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes uncharacterized LOC101162913 (LOC101162913), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011473328.2

ORF Insert Sequence:

ATGCAGAGTC CATCTTTGAC CCGGACGGAC TCCATCCCTG CCTCCTCATC GGCCTCAGAG 
CAGCCCGTGG ACATGGTGGC TGAGCCGGAG CAGCAGGGAC CAGAGGGACA GGCGGGGGAC 
CACCCGGCAG TGGCTCCTGC AGACCCCCAA CCACCAGAGA CGAGTCAACA CACCCAAGGG 
GGAGCACAGA GCCCTGCAAA TGTAGCGGCC ATGCCAACAG CAGAGGCTGA AGATGATGCC 
AAGAGAAGCA CTGAGAGCAG CAGTGGAGCG AAGACAAACG TGCAGACCGT CAGGAGATAC 
GTCCCTTCCA AGAAAGCAAT GATAGATCCT CTGAAGATGG ATATGACCAA ATCGCTGGTT 
GAGCCACTCT CATCCTCTCA GCTCTCTCTG CACTGCGTCG AGTGCCACAT CATCTTCAGT 
GACCACAAGA GCAAAGACCG CCACGTGAAG CTGAAACACC CAGTAGAATA TGAAAAGCGC 
AACCTGAAAA ACGCCCTTTT TGCATGTTAC GTGTGTGACC GCCATTTTAC CAACTCCACG 
GACCTCATGG CCCACCAGAA GGCCCACACT GAGAAGAAAC CCTTCAAGTG TCCCGTGTGC 
GATCAGGCCT TCAGGAGGTC CTCCGAGCTC ACCAGTCATA AAAAAGCTCA TTTCGGCCTG 
TTCGGCTACA CCTGCACGGA GTGCGGCAAA CCCTGCAAAA CGCTGACATT GCTGAAGTAT 
CACCAGCGTA CGCACACAGG AGAGAGGCCG TACGTCTGCA AGGAGTGTGG GAAGAGGTTC 
CCCATGCCGA AAGCTTTGCA CAAACACATG ATCTCCCACT CTCCAGAGGG AGCTGAAGGA 
GAAGAAAAAG ACGACGCTGC CAAAAAACGA GCAAAGAAAG ATGAAGGTTC TCCTCCTGTC 
AAACTCCACT GCTCCATGTG CAAGGCCACT TTCAAGAACT CCAAGACTCG CCAGCGGCAC 
ATGAAAATCA AGCACAACGT CCTCCCATCA GGGCAAAGCA AACCTGTTAT CACCCCCATT 
TCGATCTCAC AGCCCACGCG GTTGCAGCTA GAGGCCGCTG GACCTCTGCA AAAAGTTGAT 
GCCAACATCG ACACAGCGCA AATACGCAAA CTTATAGAGT CGCTGGGCAC TGTGAAGAAA 
GTGAACCAAG TGGTTCTGTT GGGTCAGGTG CCGGGTTACG CTCCACCACT GGAATTGCAG 
GCGGATTCGC AGCCAGCAGG GCCTCTGGAT CCCGATCTAA GTCCACCGCA GATCGATTTT 
GGAGGACTGA AGCCGCCCGA ATCCAAGACG ACTGGACTGG ACACTTCCAA TTATCCGTGT 
GGGTTCATGG ACCAAACCAT CATTCTGGAA CCCATCACTC CTGATGGAGA AACCCCCCCC 
CTGTCAGAGC TGGCATCCTG TGTAGCAGCA GGCGAAACTG CACAGACTGT GGGAGAGGTG 
GTTCAGAGGC CCCTGACCAG TGAAATTCTC TTGGATAATA TGACCTGCCA GAACGAGGAG 
AATGACCTTG AACAAACATT CATATTAGAA CTCACCCCAG CTTTGACACC AACTGCAGAA 
CTGGAGCACT CCCAGACTGA ACCACAGAAT ATGGCTCCGT CCTCCTCTCT GGTGCTGAAC 
ACGGACCAGG AGGCTGCAGA TCAAACTGCC AAAAAGTCTG CCCTCAATGG GCAAGACGCC 
AGCCTGACAG AACCGCCACT TGTCTCCACA GCCCAGCTGG ATGGAACATC TCCTTCCTTA 
GACCCAGAGC ACAAAGAACT TCCCTCCAAC CCTTTGGATT CACCAGATAA ACCCTCACAA 
GTTGGCCCCA ATGAAGGCGA TGATGCATGT ACGAAGATGA CGGGTCAAGA CCAGTTGAGT 
GAGGGCGAAT TAAAGACACA GGAACACAAT GGAGAGCATT TGTCTGAGAA ATCTGGAATG 
GAAGGTAAGA AGAACCATTC AGAAGTGGAG TCGTCTTCTA AAGCGGCAGT CCCGTCTGAC 
ATGCAGTCAA AACATGTTCC TCAGACCTCA GAACTGGCCC TGAATGTCAT GTCTGCTCAG 
GAGCTGGTGA AAGTGCGCAA AAGAAAGCCG GCCAGGACGT TTTTCTTTCC AGGATACATT 
CAAGATCTGG CCGGGTCCAT TTACAAAGAC GACTTTCTGA TCGACGCCAA ACCAGCCAAG 
CGGCAAAGAA CAAAAAAGTC TCATCTTGTT GTGAAATTCA GTCCTCAGAG CAAAGACAAG 
AAAAATAAGA AAAAAAAGCA GGAACCGCAG CAGCCAAGAC CAGAAGATCT GACTAAAGGT 
AAAGCTCCGG CTAAGAGAAC ACCTGAGAAA AAGATGTCCT TGTCACCAAA GAAAGGAGCA 
AAGGGAAAAA AGGATGCTGC AGGGGGGGGC GGAGCATCAG GAGTTGTCAC AAAGTCCCCT 
CCAACCCCAG TTGCACAGGA GAAACAAATC AAAGAAGATG TGGGTAAAAA CAAACTAAAA 
AAAAAAAAAG TAGGGAACGA AGATGCACAA ACGAGTGGGC AGAAGGCCGC AGCTTCACCT 
GTGTTCAAAA AGAAAAAGCA ACCAAAGGTT AGACAAGAAG TTTCACCCAA AAGTGCAAAA 
ACTGAAAGAG CTAGGAAAAA AACACCAACC AAAAAAGTCA AAAAGCCAGA AGAACCGAAT 
TTAAACGCAG TACAAGACTC TCTGCTTTTA TTAAAAGGCC ATAAGCAGCC CCAGCTCAAA 
GTTTACAAGC TGGACCCTTC CAGGGCGTCG AGTCAGCCTC AGGACGCCTC CGTCTCCAAG 
AAAAAGGGAG GACGGGCAAA GAAAGCTCTG TCCCTCATGT CTTCCTTGAA GTCTTCTCAA 
CCTCCACCCG ATGCTCCGCC CAACAAGCCA AAGGCCACCA GAAAACGCAA AGCCTCGTCG 
AAAGTGGAAA CTGAAGGAGT CATAACCTCT AAGCGAGCCT TGGAGTGCAA AGACTGTGGG 
GAAAAGTTCA GTGACGTCTC ATCGCTTCAG AAGCACAAAA CGACCGCACA CACCGTAGAG 
AGTCCAAGTC TCACCTACAC CAACGGAAAC ATATTTGAGG GCGTCTCCAG GTTGGAGTTT 
TACCCGTCCC CGAAGGTGTA TGACGGCGGA GCCGCGGGAA AGAATGCAGC AACAGGGTGG 
GACACTGAGC CAGAGATGGG AGAGATGACT GTGGAGGACA GGGAGCAGAA TGTCTCTTTC 
CCAGCTCTGA TTCCATCCCC ATCTCTGCCG GTTTCTCCTC CAAATGTTGA CATAAATGCC 
TTCCAGGGGG CAAGTAGAAG AGACGAGAAC AAAGCCTCCC CTTTAGATGT TTGCTCTCGG 
CCCCGTGACA TGACTGCTGG CAAGGATCGC ACAGATTTCC TAGAGTCCGC TCAGGCGAAG 
TCCTCTGAGA GGAGGCCGCT GTTTCCACCA GAATGTGCCT CAAAGAGCCC CAACTCTGAA 
TGTGAAGCCC AGGGAACCAC CGAGGAAGAC TTCAAGGAAG ATCTACTTCT AGAGGTTGAC 
TTGGTTACTG TTGGGGAACA GAATGAGAGA GACAGAAGTT CTCTGGATGA AGACACTTCT 
ACCCAAAAAG ATTTAAACAC CAGTGATTCT GAAAGTGATA AACACCCCGA GCAGATTTGT 
GATGGAAGGA GTGAAAATGA GGTAACTTCA AAGACTGTGT TTTGCTCAAC CAATCAGGTG 
AAAGAAGAAG AGGAAGAGAT GTTGGTGCAG AGGAGAGGAG AGGACCAGAA GAGCGTTGTG 
ATGAGTAGAC GTAGGAGAAG AGGCTCAGAG CCCAGAAGAG GAGATGCCAT AAGAAAGACG 
GGATCTGTTG GAGAGTCTCA GACGGAAACT GAAAGTGAGC AGGATGAATG TCAGGTGGTT 
TATGAGATAC ACTCCGTCTT CTCGGACCAG GAGTCTGACA CGAAGACCAG CCCTGGATTC 
AGGACCATGA AAGGCCCTGC TTCTGTTGCG TTCATCCCTT CTGGTCCTTC AGCGTTTGAC 
AAATCCTCTG AAGAGCGTGT AGTGCTGGAG CTGGAGTCTG TAACCACTAG TGTTCAGGAA 
GTGATGAACG AACACAGGCT GCATGGAGAC GGGGAGACGG ACAGGGAAGC AGACCAGTCT 
CCAGGCATCG TGCTGGAGAG GTTCCTGACC TCCAGACAAA GATCTTCTGA CAAAGAGGCC 
TCCTGCAGGG CTGCAGACCA ACGAGAGGGT TTTGATGGTG TTTCTGAGAA AGAAGTACAG 
TTTGTTGGGA GCCAGGAGAT CAAAGTTGAA GAGACCCCGC CGGACCCCAC ACTGCAGCCC 
CCTGCCTGTC AAAGCAGGCA GAGGGCCGGT CTCCATCCAC AGCATAATCG AGCCATACGG 
ACCGTTCTAG TGAAAGAGGA GAGCGGCCCC CTGCTGAACG CTGCTCTGGA CGCCCAAGGC 
AGCAGACGGA TGCAGTGGGC AGTGGAGCCA ATCAGCAGCG GGGGGGCCGA TGGGCCGTTA 
ATTGAGAATG GGCAGAGCCC CAATGAGGCA CGCAGGAGTC CAGAGTTCAA CACCAAGCAG 
TGCATCTTCT ATCCAGTGAA GGAGGAGGAG CGGGAACTTC TGCTGGCATC TGCTCAGACC 
AACGCTGACA CCTTCAGCCT GGAAGGCTTC GGTGATGGAG CCCCAGCTGA GCATCAACCT 
GCAGAGTGTG ATGCATATGG AGGAAGACTC TCTGCTGCAG GCTACCAGAA TGGAAGCCTG 
CTGTCAGAAC CAGAGACTTC AGACTTCACT GACAGTCAAG CTTTCTCTGA CTCGGAGTGG 
CAGCCGTCAG ATGTGCGGGA CTTTCTACTC AAGAGTTGTG ATGACGACGT GAGCGGCTTC 
GAACTGCCTG ATCCTCAGCG TGACACAGAA GCAGAAGTGA TGGCTTACTT CAACAGGAAG 
CAGTCCAGCA GCTCGCATGA GCCCAATCAC ACATCCGCGA GTTTTTCAAC ATCGAGTCAT 
CTGCAACCCC AGATGAATGG AACCAGGACC AGAGAGCCCA TTGATTACTT TTCCAAATAT 
TTTGGTTGGG ATACTTGGGC AGAAACTTCA ACTTGCACAA ACAAACTCTG CAAAATGTCA 
GATCCTGTCA CGTCTGTAGA CGTTGCACGG TTTGTTGGTA TCCACATCGC CATGGGAACT 
TTGAAGTTTC CCAGTCCAAG GCTTTACTGG GAGGACTTGA CGAAGGTGCC CCTGATTGCT 
GACGCCATGC CCCTGACTCG CTTTGTGGAG CTGTCCCGTA CGTTACAGCT ACCTTCTCCT 
GCTGAAGAGC AGCCACCTGG TGATGGACAG TCCAGAGATG ACGAGGGGAG GGACTTGAAC 
TGTTCACCAA CACAGATGGA TCCTCTGTGG AAGGTCCAGC CAATCCTGGG CCGCTTCAAT 
GCTGAGTGCC AGTCGTTGAG AAAGGAGGAC AACTATGCCG TTGACCAGTA TCCACTTCCT 
CTGACCTGCA AGACTCGCGA AAGTGGGCCG TCTCTGCTCT GCACTGCACT GATTGGACTT 
GGTGGTTTAC TCTTGCATGT GGATCTCAAT GTGGATCCCC CAGATAAAGA AGGTGTCGTA 
GAAAAGATGG CGCCTAAAAG TAGCATGGTG TTTCTTTGCA AACAGGAGCT TTCCACCCCT 
GCCATGATGG AACGCCTTTT AGATGCTGGG GTTCACGCGG CCGGTAAGGT GGGAGGAGCT 
CGAGGACAAA TCGGAGATGA GTTTGTGAGC TCCGACGGGA AGCTGATGCT CCGCAGATCC 
CACAGCGGCT TCATACTTTC TACAGCGGGG AACGGTCGGA GGAGCATGGC TTCCCTCACC 
AGCAGCTTCC AGAAGGCCCA GATGTCGGCT CGCCTCAACA GAGATTTGCT AAACCTGTAC 
TCCACACCTC TCACGGCCAC GCCTCCACAC AGCTGGCCCC AAGCGGTGCT CTGGTACCTC 
ATCGATTTGG CTTTGGTGAA CTCCTGGCTT TTGTACCGAG AAGATCACGG GGCAGCTGCT 
GGAGCTTTGA CTTTCATGGC TTTCAGGCTG GATGTTTCCA AGGCGCTGAC CCTCTCCGGC 
AGCTCAGACA CACAGGACTC CATTCCTCCT CAGCCTCCAT CAGAGGAAAC CCACGGGACA 
AGCCAAACGC CCAGTCCCAT GCTGGTGGAG GACAGCCCCC TGCCCGACGC CGCCACCCGC 
TACGACGGCG CAGGCCACTG GCCGGAGCAG CTGGCAGAGG GGGAAGGGGG CAGGTGTCGT 
TTCGGGGACT GTCAGAGGAC GTCGCGAGTT TTGTGTCTGA AGTGTTGCGT CTTTCTCTGC 
ATCTCACGAA ATCACAACTG CTTTTTGAAT TTTCATAACC AGGACAATAT AGGCAGAAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101162913 ( XM_020701850.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020557509.1 Oryzias latipes uncharacterized LOC101162913 (LOC101162913), transcript variant X2, mRNA.

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CloneID	OJa27422
Clone ID Related Accession (Same CDS sequence)	XM_011473328.3 , XM_011473328.2 , XM_020701850.1 , XM_020701850.2
Accession Version	XM_020701850.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	uncharacterized protein LOC101162913
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004095190.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGTC CATCTTTGAC CCGGACGGAC TCCATCCCTG CCTCCTCATC GGCCTCAGAG 
CAGCCCGTGG ACATGGTGGC TGAGCCGGAG CAGCAGGGAC CAGAGGGACA GGCGGGGGAC 
CACCCGGCAG TGGCTCCTGC AGACCCCCAA CCACCAGAGA CGAGTCAACA CACCCAAGGG 
GGAGCACAGA GCCCTGCAAA TGTAGCGGCC ATGCCAACAG CAGAGGCTGA AGATGATGCC 
AAGAGAAGCA CTGAGAGCAG CAGTGGAGCG AAGACAAACG TGCAGACCGT CAGGAGATAC 
GTCCCTTCCA AGAAAGCAAT GATAGATCCT CTGAAGATGG ATATGACCAA ATCGCTGGTT 
GAGCCACTCT CATCCTCTCA GCTCTCTCTG CACTGCGTCG AGTGCCACAT CATCTTCAGT 
GACCACAAGA GCAAAGACCG CCACGTGAAG CTGAAACACC CAGTAGAATA TGAAAAGCGC 
AACCTGAAAA ACGCCCTTTT TGCATGTTAC GTGTGTGACC GCCATTTTAC CAACTCCACG 
GACCTCATGG CCCACCAGAA GGCCCACACT GAGAAGAAAC CCTTCAAGTG TCCCGTGTGC 
GATCAGGCCT TCAGGAGGTC CTCCGAGCTC ACCAGTCATA AAAAAGCTCA TTTCGGCCTG 
TTCGGCTACA CCTGCACGGA GTGCGGCAAA CCCTGCAAAA CGCTGACATT GCTGAAGTAT 
CACCAGCGTA CGCACACAGG AGAGAGGCCG TACGTCTGCA AGGAGTGTGG GAAGAGGTTC 
CCCATGCCGA AAGCTTTGCA CAAACACATG ATCTCCCACT CTCCAGAGGG AGCTGAAGGA 
GAAGAAAAAG ACGACGCTGC CAAAAAACGA GCAAAGAAAG ATGAAGGTTC TCCTCCTGTC 
AAACTCCACT GCTCCATGTG CAAGGCCACT TTCAAGAACT CCAAGACTCG CCAGCGGCAC 
ATGAAAATCA AGCACAACGT CCTCCCATCA GGGCAAAGCA AACCTGTTAT CACCCCCATT 
TCGATCTCAC AGCCCACGCG GTTGCAGCTA GAGGCCGCTG GACCTCTGCA AAAAGTTGAT 
GCCAACATCG ACACAGCGCA AATACGCAAA CTTATAGAGT CGCTGGGCAC TGTGAAGAAA 
GTGAACCAAG TGGTTCTGTT GGGTCAGGTG CCGGGTTACG CTCCACCACT GGAATTGCAG 
GCGGATTCGC AGCCAGCAGG GCCTCTGGAT CCCGATCTAA GTCCACCGCA GATCGATTTT 
GGAGGACTGA AGCCGCCCGA ATCCAAGACG ACTGGACTGG ACACTTCCAA TTATCCGTGT 
GGGTTCATGG ACCAAACCAT CATTCTGGAA CCCATCACTC CTGATGGAGA AACCCCCCCC 
CTGTCAGAGC TGGCATCCTG TGTAGCAGCA GGCGAAACTG CACAGACTGT GGGAGAGGTG 
GTTCAGAGGC CCCTGACCAG TGAAATTCTC TTGGATAATA TGACCTGCCA GAACGAGGAG 
AATGACCTTG AACAAACATT CATATTAGAA CTCACCCCAG CTTTGACACC AACTGCAGAA 
CTGGAGCACT CCCAGACTGA ACCACAGAAT ATGGCTCCGT CCTCCTCTCT GGTGCTGAAC 
ACGGACCAGG AGGCTGCAGA TCAAACTGCC AAAAAGTCTG CCCTCAATGG GCAAGACGCC 
AGCCTGACAG AACCGCCACT TGTCTCCACA GCCCAGCTGG ATGGAACATC TCCTTCCTTA 
GACCCAGAGC ACAAAGAACT TCCCTCCAAC CCTTTGGATT CACCAGATAA ACCCTCACAA 
GTTGGCCCCA ATGAAGGCGA TGATGCATGT ACGAAGATGA CGGGTCAAGA CCAGTTGAGT 
GAGGGCGAAT TAAAGACACA GGAACACAAT GGAGAGCATT TGTCTGAGAA ATCTGGAATG 
GAAGGTAAGA AGAACCATTC AGAAGTGGAG TCGTCTTCTA AAGCGGCAGT CCCGTCTGAC 
ATGCAGTCAA AACATGTTCC TCAGACCTCA GAACTGGCCC TGAATGTCAT GTCTGCTCAG 
GAGCTGGTGA AAGTGCGCAA AAGAAAGCCG GCCAGGACGT TTTTCTTTCC AGGATACATT 
CAAGATCTGG CCGGGTCCAT TTACAAAGAC GACTTTCTGA TCGACGCCAA ACCAGCCAAG 
CGGCAAAGAA CAAAAAAGTC TCATCTTGTT GTGAAATTCA GTCCTCAGAG CAAAGACAAG 
AAAAATAAGA AAAAAAAGCA GGAACCGCAG CAGCCAAGAC CAGAAGATCT GACTAAAGGT 
AAAGCTCCGG CTAAGAGAAC ACCTGAGAAA AAGATGTCCT TGTCACCAAA GAAAGGAGCA 
AAGGGAAAAA AGGATGCTGC AGGGGGGGGC GGAGCATCAG GAGTTGTCAC AAAGTCCCCT 
CCAACCCCAG TTGCACAGGA GAAACAAATC AAAGAAGATG TGGGTAAAAA CAAACTAAAA 
AAAAAAAAAG TAGGGAACGA AGATGCACAA ACGAGTGGGC AGAAGGCCGC AGCTTCACCT 
GTGTTCAAAA AGAAAAAGCA ACCAAAGGTT AGACAAGAAG TTTCACCCAA AAGTGCAAAA 
ACTGAAAGAG CTAGGAAAAA AACACCAACC AAAAAAGTCA AAAAGCCAGA AGAACCGAAT 
TTAAACGCAG TACAAGACTC TCTGCTTTTA TTAAAAGGCC ATAAGCAGCC CCAGCTCAAA 
GTTTACAAGC TGGACCCTTC CAGGGCGTCG AGTCAGCCTC AGGACGCCTC CGTCTCCAAG 
AAAAAGGGAG GACGGGCAAA GAAAGCTCTG TCCCTCATGT CTTCCTTGAA GTCTTCTCAA 
CCTCCACCCG ATGCTCCGCC CAACAAGCCA AAGGCCACCA GAAAACGCAA AGCCTCGTCG 
AAAGTGGAAA CTGAAGGAGT CATAACCTCT AAGCGAGCCT TGGAGTGCAA AGACTGTGGG 
GAAAAGTTCA GTGACGTCTC ATCGCTTCAG AAGCACAAAA CGACCGCACA CACCGTAGAG 
AGTCCAAGTC TCACCTACAC CAACGGAAAC ATATTTGAGG GCGTCTCCAG GTTGGAGTTT 
TACCCGTCCC CGAAGGTGTA TGACGGCGGA GCCGCGGGAA AGAATGCAGC AACAGGGTGG 
GACACTGAGC CAGAGATGGG AGAGATGACT GTGGAGGACA GGGAGCAGAA TGTCTCTTTC 
CCAGCTCTGA TTCCATCCCC ATCTCTGCCG GTTTCTCCTC CAAATGTTGA CATAAATGCC 
TTCCAGGGGG CAAGTAGAAG AGACGAGAAC AAAGCCTCCC CTTTAGATGT TTGCTCTCGG 
CCCCGTGACA TGACTGCTGG CAAGGATCGC ACAGATTTCC TAGAGTCCGC TCAGGCGAAG 
TCCTCTGAGA GGAGGCCGCT GTTTCCACCA GAATGTGCCT CAAAGAGCCC CAACTCTGAA 
TGTGAAGCCC AGGGAACCAC CGAGGAAGAC TTCAAGGAAG ATCTACTTCT AGAGGTTGAC 
TTGGTTACTG TTGGGGAACA GAATGAGAGA GACAGAAGTT CTCTGGATGA AGACACTTCT 
ACCCAAAAAG ATTTAAACAC CAGTGATTCT GAAAGTGATA AACACCCCGA GCAGATTTGT 
GATGGAAGGA GTGAAAATGA GGTAACTTCA AAGACTGTGT TTTGCTCAAC CAATCAGGTG 
AAAGAAGAAG AGGAAGAGAT GTTGGTGCAG AGGAGAGGAG AGGACCAGAA GAGCGTTGTG 
ATGAGTAGAC GTAGGAGAAG AGGCTCAGAG CCCAGAAGAG GAGATGCCAT AAGAAAGACG 
GGATCTGTTG GAGAGTCTCA GACGGAAACT GAAAGTGAGC AGGATGAATG TCAGGTGGTT 
TATGAGATAC ACTCCGTCTT CTCGGACCAG GAGTCTGACA CGAAGACCAG CCCTGGATTC 
AGGACCATGA AAGGCCCTGC TTCTGTTGCG TTCATCCCTT CTGGTCCTTC AGCGTTTGAC 
AAATCCTCTG AAGAGCGTGT AGTGCTGGAG CTGGAGTCTG TAACCACTAG TGTTCAGGAA 
GTGATGAACG AACACAGGCT GCATGGAGAC GGGGAGACGG ACAGGGAAGC AGACCAGTCT 
CCAGGCATCG TGCTGGAGAG GTTCCTGACC TCCAGACAAA GATCTTCTGA CAAAGAGGCC 
TCCTGCAGGG CTGCAGACCA ACGAGAGGGT TTTGATGGTG TTTCTGAGAA AGAAGTACAG 
TTTGTTGGGA GCCAGGAGAT CAAAGTTGAA GAGACCCCGC CGGACCCCAC ACTGCAGCCC 
CCTGCCTGTC AAAGCAGGCA GAGGGCCGGT CTCCATCCAC AGCATAATCG AGCCATACGG 
ACCGTTCTAG TGAAAGAGGA GAGCGGCCCC CTGCTGAACG CTGCTCTGGA CGCCCAAGGC 
AGCAGACGGA TGCAGTGGGC AGTGGAGCCA ATCAGCAGCG GGGGGGCCGA TGGGCCGTTA 
ATTGAGAATG GGCAGAGCCC CAATGAGGCA CGCAGGAGTC CAGAGTTCAA CACCAAGCAG 
TGCATCTTCT ATCCAGTGAA GGAGGAGGAG CGGGAACTTC TGCTGGCATC TGCTCAGACC 
AACGCTGACA CCTTCAGCCT GGAAGGCTTC GGTGATGGAG CCCCAGCTGA GCATCAACCT 
GCAGAGTGTG ATGCATATGG AGGAAGACTC TCTGCTGCAG GCTACCAGAA TGGAAGCCTG 
CTGTCAGAAC CAGAGACTTC AGACTTCACT GACAGTCAAG CTTTCTCTGA CTCGGAGTGG 
CAGCCGTCAG ATGTGCGGGA CTTTCTACTC AAGAGTTGTG ATGACGACGT GAGCGGCTTC 
GAACTGCCTG ATCCTCAGCG TGACACAGAA GCAGAAGTGA TGGCTTACTT CAACAGGAAG 
CAGTCCAGCA GCTCGCATGA GCCCAATCAC ACATCCGCGA GTTTTTCAAC ATCGAGTCAT 
CTGCAACCCC AGATGAATGG AACCAGGACC AGAGAGCCCA TTGATTACTT TTCCAAATAT 
TTTGGTTGGG ATACTTGGGC AGAAACTTCA ACTTGCACAA ACAAACTCTG CAAAATGTCA 
GATCCTGTCA CGTCTGTAGA CGTTGCACGG TTTGTTGGTA TCCACATCGC CATGGGAACT 
TTGAAGTTTC CCAGTCCAAG GCTTTACTGG GAGGACTTGA CGAAGGTGCC CCTGATTGCT 
GACGCCATGC CCCTGACTCG CTTTGTGGAG CTGTCCCGTA CGTTACAGCT ACCTTCTCCT 
GCTGAAGAGC AGCCACCTGG TGATGGACAG TCCAGAGATG ACGAGGGGAG GGACTTGAAC 
TGTTCACCAA CACAGATGGA TCCTCTGTGG AAGGTCCAGC CAATCCTGGG CCGCTTCAAT 
GCTGAGTGCC AGTCGTTGAG AAAGGAGGAC AACTATGCCG TTGACCAGTA TCCACTTCCT 
CTGACCTGCA AGACTCGCGA AAGTGGGCCG TCTCTGCTCT GCACTGCACT GATTGGACTT 
GGTGGTTTAC TCTTGCATGT GGATCTCAAT GTGGATCCCC CAGATAAAGA AGGTGTCGTA 
GAAAAGATGG CGCCTAAAAG TAGCATGGTG TTTCTTTGCA AACAGGAGCT TTCCACCCCT 
GCCATGATGG AACGCCTTTT AGATGCTGGG GTTCACGCGG CCGGTAAGGT GGGAGGAGCT 
CGAGGACAAA TCGGAGATGA GTTTGTGAGC TCCGACGGGA AGCTGATGCT CCGCAGATCC 
CACAGCGGCT TCATACTTTC TACAGCGGGG AACGGTCGGA GGAGCATGGC TTCCCTCACC 
AGCAGCTTCC AGAAGGCCCA GATGTCGGCT CGCCTCAACA GAGATTTGCT AAACCTGTAC 
TCCACACCTC TCACGGCCAC GCCTCCACAC AGCTGGCCCC AAGCGGTGCT CTGGTACCTC 
ATCGATTTGG CTTTGGTGAA CTCCTGGCTT TTGTACCGAG AAGATCACGG GGCAGCTGCT 
GGAGCTTTGA CTTTCATGGC TTTCAGGCTG GATGTTTCCA AGGCGCTGAC CCTCTCCGGC 
AGCTCAGACA CACAGGACTC CATTCCTCCT CAGCCTCCAT CAGAGGAAAC CCACGGGACA 
AGCCAAACGC CCAGTCCCAT GCTGGTGGAG GACAGCCCCC TGCCCGACGC CGCCACCCGC 
TACGACGGCG CAGGCCACTG GCCGGAGCAG CTGGCAGAGG GGGAAGGGGG CAGGTGTCGT 
TTCGGGGACT GTCAGAGGAC GTCGCGAGTT TTGTGTCTGA AGTGTTGCGT CTTTCTCTGC 
ATCTCACGAA ATCACAACTG CTTTTTGAAT TTTCATAACC AGGACAATAT AGGCAGAAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020557509.1
CDS	109..6411
Translation	MQSPSLTRTDSIPASSSASEQPVDMVAEPEQQGPEGQAGDHPAVAPADPQPPETSQHTQGGAQSPANVAAMPTAEAEDDAKRSTESSSGAKTNVQTVRRYVPSKKAMIDPLKMDMTKSLVEPLSSSQLSLHCVECHIIFSDHKSKDRHVKLKHPVEYEKRNLKNALFACYVCDRHFTNSTDLMAHQKAHTEKKPFKCPVCDQAFRRSSELTSHKKAHFGLFGYTCTECGKPCKTLTLLKYHQRTHTGERPYVCKECGKRFPMPKALHKHMISHSPEGAEGEEKDDAAKKRAKKDEGSPPVKLHCSMCKATFKNSKTRQRHMKIKHNVLPSGQSKPVITPISISQPTRLQLEAAGPLQKVDANIDTAQIRKLIESLGTVKKVNQVVLLGQVPGYAPPLELQADSQPAGPLDPDLSPPQIDFGGLKPPESKTTGLDTSNYPCGFMDQTIILEPITPDGETPPLSELASCVAAGETAQTVGEVVQRPLTSEILLDNMTCQNEENDLEQTFILELTPALTPTAELEHSQTEPQNMAPSSSLVLNTDQEAADQTAKKSALNGQDASLTEPPLVSTAQLDGTSPSLDPEHKELPSNPLDSPDKPSQVGPNEGDDACTKMTGQDQLSEGELKTQEHNGEHLSEKSGMEGKKNHSEVESSSKAAVPSDMQSKHVPQTSELALNVMSAQELVKVRKRKPARTFFFPGYIQDLAGSIYKDDFLIDAKPAKRQRTKKSHLVVKFSPQSKDKKNKKKKQEPQQPRPEDLTKGKAPAKRTPEKKMSLSPKKGAKGKKDAAGGGGASGVVTKSPPTPVAQEKQIKEDVGKNKLKKKKVGNEDAQTSGQKAAASPVFKKKKQPKVRQEVSPKSAKTERARKKTPTKKVKKPEEPNLNAVQDSLLLLKGHKQPQLKVYKLDPSRASSQPQDASVSKKKGGRAKKALSLMSSLKSSQPPPDAPPNKPKATRKRKASSKVETEGVITSKRALECKDCGEKFSDVSSLQKHKTTAHTVESPSLTYTNGNIFEGVSRLEFYPSPKVYDGGAAGKNAATGWDTEPEMGEMTVEDREQNVSFPALIPSPSLPVSPPNVDINAFQGASRRDENKASPLDVCSRPRDMTAGKDRTDFLESAQAKSSERRPLFPPECASKSPNSECEAQGTTEEDFKEDLLLEVDLVTVGEQNERDRSSLDEDTSTQKDLNTSDSESDKHPEQICDGRSENEVTSKTVFCSTNQVKEEEEEMLVQRRGEDQKSVVMSRRRRRGSEPRRGDAIRKTGSVGESQTETESEQDECQVVYEIHSVFSDQESDTKTSPGFRTMKGPASVAFIPSGPSAFDKSSEERVVLELESVTTSVQEVMNEHRLHGDGETDREADQSPGIVLERFLTSRQRSSDKEASCRAADQREGFDGVSEKEVQFVGSQEIKVEETPPDPTLQPPACQSRQRAGLHPQHNRAIRTVLVKEESGPLLNAALDAQGSRRMQWAVEPISSGGADGPLIENGQSPNEARRSPEFNTKQCIFYPVKEEERELLLASAQTNADTFSLEGFGDGAPAEHQPAECDAYGGRLSAAGYQNGSLLSEPETSDFTDSQAFSDSEWQPSDVRDFLLKSCDDDVSGFELPDPQRDTEAEVMAYFNRKQSSSSHEPNHTSASFSTSSHLQPQMNGTRTREPIDYFSKYFGWDTWAETSTCTNKLCKMSDPVTSVDVARFVGIHIAMGTLKFPSPRLYWEDLTKVPLIADAMPLTRFVELSRTLQLPSPAEEQPPGDGQSRDDEGRDLNCSPTQMDPLWKVQPILGRFNAECQSLRKEDNYAVDQYPLPLTCKTRESGPSLLCTALIGLGGLLLHVDLNVDPPDKEGVVEKMAPKSSMVFLCKQELSTPAMMERLLDAGVHAAGKVGGARGQIGDEFVSSDGKLMLRRSHSGFILSTAGNGRRSMASLTSSFQKAQMSARLNRDLLNLYSTPLTATPPHSWPQAVLWYLIDLALVNSWLLYREDHGAAAGALTFMAFRLDVSKALTLSGSSDTQDSIPPQPPSEETHGTSQTPSPMLVEDSPLPDAATRYDGAGHWPEQLAEGEGGRCRFGDCQRTSRVLCLKCCVFLCISRNHNCFLNFHNQDNIGRK

Target ORF information:

RefSeq Version	XM_020701850.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes uncharacterized LOC101162913 (LOC101162913), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020701850.1

ORF Insert Sequence:

ATGCAGAGTC CATCTTTGAC CCGGACGGAC TCCATCCCTG CCTCCTCATC GGCCTCAGAG 
CAGCCCGTGG ACATGGTGGC TGAGCCGGAG CAGCAGGGAC CAGAGGGACA GGCGGGGGAC 
CACCCGGCAG TGGCTCCTGC AGACCCCCAA CCACCAGAGA CGAGTCAACA CACCCAAGGG 
GGAGCACAGA GCCCTGCAAA TGTAGCGGCC ATGCCAACAG CAGAGGCTGA AGATGATGCC 
AAGAGAAGCA CTGAGAGCAG CAGTGGAGCG AAGACAAACG TGCAGACCGT CAGGAGATAC 
GTCCCTTCCA AGAAAGCAAT GATAGATCCT CTGAAGATGG ATATGACCAA ATCGCTGGTT 
GAGCCACTCT CATCCTCTCA GCTCTCTCTG CACTGCGTCG AGTGCCACAT CATCTTCAGT 
GACCACAAGA GCAAAGACCG CCACGTGAAG CTGAAACACC CAGTAGAATA TGAAAAGCGC 
AACCTGAAAA ACGCCCTTTT TGCATGTTAC GTGTGTGACC GCCATTTTAC CAACTCCACG 
GACCTCATGG CCCACCAGAA GGCCCACACT GAGAAGAAAC CCTTCAAGTG TCCCGTGTGC 
GATCAGGCCT TCAGGAGGTC CTCCGAGCTC ACCAGTCATA AAAAAGCTCA TTTCGGCCTG 
TTCGGCTACA CCTGCACGGA GTGCGGCAAA CCCTGCAAAA CGCTGACATT GCTGAAGTAT 
CACCAGCGTA CGCACACAGG AGAGAGGCCG TACGTCTGCA AGGAGTGTGG GAAGAGGTTC 
CCCATGCCGA AAGCTTTGCA CAAACACATG ATCTCCCACT CTCCAGAGGG AGCTGAAGGA 
GAAGAAAAAG ACGACGCTGC CAAAAAACGA GCAAAGAAAG ATGAAGGTTC TCCTCCTGTC 
AAACTCCACT GCTCCATGTG CAAGGCCACT TTCAAGAACT CCAAGACTCG CCAGCGGCAC 
ATGAAAATCA AGCACAACGT CCTCCCATCA GGGCAAAGCA AACCTGTTAT CACCCCCATT 
TCGATCTCAC AGCCCACGCG GTTGCAGCTA GAGGCCGCTG GACCTCTGCA AAAAGTTGAT 
GCCAACATCG ACACAGCGCA AATACGCAAA CTTATAGAGT CGCTGGGCAC TGTGAAGAAA 
GTGAACCAAG TGGTTCTGTT GGGTCAGGTG CCGGGTTACG CTCCACCACT GGAATTGCAG 
GCGGATTCGC AGCCAGCAGG GCCTCTGGAT CCCGATCTAA GTCCACCGCA GATCGATTTT 
GGAGGACTGA AGCCGCCCGA ATCCAAGACG ACTGGACTGG ACACTTCCAA TTATCCGTGT 
GGGTTCATGG ACCAAACCAT CATTCTGGAA CCCATCACTC CTGATGGAGA AACCCCCCCC 
CTGTCAGAGC TGGCATCCTG TGTAGCAGCA GGCGAAACTG CACAGACTGT GGGAGAGGTG 
GTTCAGAGGC CCCTGACCAG TGAAATTCTC TTGGATAATA TGACCTGCCA GAACGAGGAG 
AATGACCTTG AACAAACATT CATATTAGAA CTCACCCCAG CTTTGACACC AACTGCAGAA 
CTGGAGCACT CCCAGACTGA ACCACAGAAT ATGGCTCCGT CCTCCTCTCT GGTGCTGAAC 
ACGGACCAGG AGGCTGCAGA TCAAACTGCC AAAAAGTCTG CCCTCAATGG GCAAGACGCC 
AGCCTGACAG AACCGCCACT TGTCTCCACA GCCCAGCTGG ATGGAACATC TCCTTCCTTA 
GACCCAGAGC ACAAAGAACT TCCCTCCAAC CCTTTGGATT CACCAGATAA ACCCTCACAA 
GTTGGCCCCA ATGAAGGCGA TGATGCATGT ACGAAGATGA CGGGTCAAGA CCAGTTGAGT 
GAGGGCGAAT TAAAGACACA GGAACACAAT GGAGAGCATT TGTCTGAGAA ATCTGGAATG 
GAAGGTAAGA AGAACCATTC AGAAGTGGAG TCGTCTTCTA AAGCGGCAGT CCCGTCTGAC 
ATGCAGTCAA AACATGTTCC TCAGACCTCA GAACTGGCCC TGAATGTCAT GTCTGCTCAG 
GAGCTGGTGA AAGTGCGCAA AAGAAAGCCG GCCAGGACGT TTTTCTTTCC AGGATACATT 
CAAGATCTGG CCGGGTCCAT TTACAAAGAC GACTTTCTGA TCGACGCCAA ACCAGCCAAG 
CGGCAAAGAA CAAAAAAGTC TCATCTTGTT GTGAAATTCA GTCCTCAGAG CAAAGACAAG 
AAAAATAAGA AAAAAAAGCA GGAACCGCAG CAGCCAAGAC CAGAAGATCT GACTAAAGGT 
AAAGCTCCGG CTAAGAGAAC ACCTGAGAAA AAGATGTCCT TGTCACCAAA GAAAGGAGCA 
AAGGGAAAAA AGGATGCTGC AGGGGGGGGC GGAGCATCAG GAGTTGTCAC AAAGTCCCCT 
CCAACCCCAG TTGCACAGGA GAAACAAATC AAAGAAGATG TGGGTAAAAA CAAACTAAAA 
AAAAAAAAAG TAGGGAACGA AGATGCACAA ACGAGTGGGC AGAAGGCCGC AGCTTCACCT 
GTGTTCAAAA AGAAAAAGCA ACCAAAGGTT AGACAAGAAG TTTCACCCAA AAGTGCAAAA 
ACTGAAAGAG CTAGGAAAAA AACACCAACC AAAAAAGTCA AAAAGCCAGA AGAACCGAAT 
TTAAACGCAG TACAAGACTC TCTGCTTTTA TTAAAAGGCC ATAAGCAGCC CCAGCTCAAA 
GTTTACAAGC TGGACCCTTC CAGGGCGTCG AGTCAGCCTC AGGACGCCTC CGTCTCCAAG 
AAAAAGGGAG GACGGGCAAA GAAAGCTCTG TCCCTCATGT CTTCCTTGAA GTCTTCTCAA 
CCTCCACCCG ATGCTCCGCC CAACAAGCCA AAGGCCACCA GAAAACGCAA AGCCTCGTCG 
AAAGTGGAAA CTGAAGGAGT CATAACCTCT AAGCGAGCCT TGGAGTGCAA AGACTGTGGG 
GAAAAGTTCA GTGACGTCTC ATCGCTTCAG AAGCACAAAA CGACCGCACA CACCGTAGAG 
AGTCCAAGTC TCACCTACAC CAACGGAAAC ATATTTGAGG GCGTCTCCAG GTTGGAGTTT 
TACCCGTCCC CGAAGGTGTA TGACGGCGGA GCCGCGGGAA AGAATGCAGC AACAGGGTGG 
GACACTGAGC CAGAGATGGG AGAGATGACT GTGGAGGACA GGGAGCAGAA TGTCTCTTTC 
CCAGCTCTGA TTCCATCCCC ATCTCTGCCG GTTTCTCCTC CAAATGTTGA CATAAATGCC 
TTCCAGGGGG CAAGTAGAAG AGACGAGAAC AAAGCCTCCC CTTTAGATGT TTGCTCTCGG 
CCCCGTGACA TGACTGCTGG CAAGGATCGC ACAGATTTCC TAGAGTCCGC TCAGGCGAAG 
TCCTCTGAGA GGAGGCCGCT GTTTCCACCA GAATGTGCCT CAAAGAGCCC CAACTCTGAA 
TGTGAAGCCC AGGGAACCAC CGAGGAAGAC TTCAAGGAAG ATCTACTTCT AGAGGTTGAC 
TTGGTTACTG TTGGGGAACA GAATGAGAGA GACAGAAGTT CTCTGGATGA AGACACTTCT 
ACCCAAAAAG ATTTAAACAC CAGTGATTCT GAAAGTGATA AACACCCCGA GCAGATTTGT 
GATGGAAGGA GTGAAAATGA GGTAACTTCA AAGACTGTGT TTTGCTCAAC CAATCAGGTG 
AAAGAAGAAG AGGAAGAGAT GTTGGTGCAG AGGAGAGGAG AGGACCAGAA GAGCGTTGTG 
ATGAGTAGAC GTAGGAGAAG AGGCTCAGAG CCCAGAAGAG GAGATGCCAT AAGAAAGACG 
GGATCTGTTG GAGAGTCTCA GACGGAAACT GAAAGTGAGC AGGATGAATG TCAGGTGGTT 
TATGAGATAC ACTCCGTCTT CTCGGACCAG GAGTCTGACA CGAAGACCAG CCCTGGATTC 
AGGACCATGA AAGGCCCTGC TTCTGTTGCG TTCATCCCTT CTGGTCCTTC AGCGTTTGAC 
AAATCCTCTG AAGAGCGTGT AGTGCTGGAG CTGGAGTCTG TAACCACTAG TGTTCAGGAA 
GTGATGAACG AACACAGGCT GCATGGAGAC GGGGAGACGG ACAGGGAAGC AGACCAGTCT 
CCAGGCATCG TGCTGGAGAG GTTCCTGACC TCCAGACAAA GATCTTCTGA CAAAGAGGCC 
TCCTGCAGGG CTGCAGACCA ACGAGAGGGT TTTGATGGTG TTTCTGAGAA AGAAGTACAG 
TTTGTTGGGA GCCAGGAGAT CAAAGTTGAA GAGACCCCGC CGGACCCCAC ACTGCAGCCC 
CCTGCCTGTC AAAGCAGGCA GAGGGCCGGT CTCCATCCAC AGCATAATCG AGCCATACGG 
ACCGTTCTAG TGAAAGAGGA GAGCGGCCCC CTGCTGAACG CTGCTCTGGA CGCCCAAGGC 
AGCAGACGGA TGCAGTGGGC AGTGGAGCCA ATCAGCAGCG GGGGGGCCGA TGGGCCGTTA 
ATTGAGAATG GGCAGAGCCC CAATGAGGCA CGCAGGAGTC CAGAGTTCAA CACCAAGCAG 
TGCATCTTCT ATCCAGTGAA GGAGGAGGAG CGGGAACTTC TGCTGGCATC TGCTCAGACC 
AACGCTGACA CCTTCAGCCT GGAAGGCTTC GGTGATGGAG CCCCAGCTGA GCATCAACCT 
GCAGAGTGTG ATGCATATGG AGGAAGACTC TCTGCTGCAG GCTACCAGAA TGGAAGCCTG 
CTGTCAGAAC CAGAGACTTC AGACTTCACT GACAGTCAAG CTTTCTCTGA CTCGGAGTGG 
CAGCCGTCAG ATGTGCGGGA CTTTCTACTC AAGAGTTGTG ATGACGACGT GAGCGGCTTC 
GAACTGCCTG ATCCTCAGCG TGACACAGAA GCAGAAGTGA TGGCTTACTT CAACAGGAAG 
CAGTCCAGCA GCTCGCATGA GCCCAATCAC ACATCCGCGA GTTTTTCAAC ATCGAGTCAT 
CTGCAACCCC AGATGAATGG AACCAGGACC AGAGAGCCCA TTGATTACTT TTCCAAATAT 
TTTGGTTGGG ATACTTGGGC AGAAACTTCA ACTTGCACAA ACAAACTCTG CAAAATGTCA 
GATCCTGTCA CGTCTGTAGA CGTTGCACGG TTTGTTGGTA TCCACATCGC CATGGGAACT 
TTGAAGTTTC CCAGTCCAAG GCTTTACTGG GAGGACTTGA CGAAGGTGCC CCTGATTGCT 
GACGCCATGC CCCTGACTCG CTTTGTGGAG CTGTCCCGTA CGTTACAGCT ACCTTCTCCT 
GCTGAAGAGC AGCCACCTGG TGATGGACAG TCCAGAGATG ACGAGGGGAG GGACTTGAAC 
TGTTCACCAA CACAGATGGA TCCTCTGTGG AAGGTCCAGC CAATCCTGGG CCGCTTCAAT 
GCTGAGTGCC AGTCGTTGAG AAAGGAGGAC AACTATGCCG TTGACCAGTA TCCACTTCCT 
CTGACCTGCA AGACTCGCGA AAGTGGGCCG TCTCTGCTCT GCACTGCACT GATTGGACTT 
GGTGGTTTAC TCTTGCATGT GGATCTCAAT GTGGATCCCC CAGATAAAGA AGGTGTCGTA 
GAAAAGATGG CGCCTAAAAG TAGCATGGTG TTTCTTTGCA AACAGGAGCT TTCCACCCCT 
GCCATGATGG AACGCCTTTT AGATGCTGGG GTTCACGCGG CCGGTAAGGT GGGAGGAGCT 
CGAGGACAAA TCGGAGATGA GTTTGTGAGC TCCGACGGGA AGCTGATGCT CCGCAGATCC 
CACAGCGGCT TCATACTTTC TACAGCGGGG AACGGTCGGA GGAGCATGGC TTCCCTCACC 
AGCAGCTTCC AGAAGGCCCA GATGTCGGCT CGCCTCAACA GAGATTTGCT AAACCTGTAC 
TCCACACCTC TCACGGCCAC GCCTCCACAC AGCTGGCCCC AAGCGGTGCT CTGGTACCTC 
ATCGATTTGG CTTTGGTGAA CTCCTGGCTT TTGTACCGAG AAGATCACGG GGCAGCTGCT 
GGAGCTTTGA CTTTCATGGC TTTCAGGCTG GATGTTTCCA AGGCGCTGAC CCTCTCCGGC 
AGCTCAGACA CACAGGACTC CATTCCTCCT CAGCCTCCAT CAGAGGAAAC CCACGGGACA 
AGCCAAACGC CCAGTCCCAT GCTGGTGGAG GACAGCCCCC TGCCCGACGC CGCCACCCGC 
TACGACGGCG CAGGCCACTG GCCGGAGCAG CTGGCAGAGG GGGAAGGGGG CAGGTGTCGT 
TTCGGGGACT GTCAGAGGAC GTCGCGAGTT TTGTGTCTGA AGTGTTGCGT CTTTCTCTGC 
ATCTCACGAA ATCACAACTG CTTTTTGAAT TTTCATAACC AGGACAATAT AGGCAGAAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101162913 ( XM_020701850.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020557509.1 Oryzias latipes uncharacterized LOC101162913 (LOC101162913), transcript variant X2, mRNA.

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CloneID	OJa27422
Clone ID Related Accession (Same CDS sequence)	XM_011473328.3 , XM_011473328.2 , XM_020701850.1 , XM_020701850.2
Accession Version	XM_020701850.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	uncharacterized protein LOC101162913 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019874.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_020701850.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGTC CATCTTTGAC CCGGACGGAC TCCATCCCTG CCTCCTCATC GGCCTCAGAG 
CAGCCCGTGG ACATGGTGGC TGAGCCGGAG CAGCAGGGAC CAGAGGGACA GGCGGGGGAC 
CACCCGGCAG TGGCTCCTGC AGACCCCCAA CCACCAGAGA CGAGTCAACA CACCCAAGGG 
GGAGCACAGA GCCCTGCAAA TGTAGCGGCC ATGCCAACAG CAGAGGCTGA AGATGATGCC 
AAGAGAAGCA CTGAGAGCAG CAGTGGAGCG AAGACAAACG TGCAGACCGT CAGGAGATAC 
GTCCCTTCCA AGAAAGCAAT GATAGATCCT CTGAAGATGG ATATGACCAA ATCGCTGGTT 
GAGCCACTCT CATCCTCTCA GCTCTCTCTG CACTGCGTCG AGTGCCACAT CATCTTCAGT 
GACCACAAGA GCAAAGACCG CCACGTGAAG CTGAAACACC CAGTAGAATA TGAAAAGCGC 
AACCTGAAAA ACGCCCTTTT TGCATGTTAC GTGTGTGACC GCCATTTTAC CAACTCCACG 
GACCTCATGG CCCACCAGAA GGCCCACACT GAGAAGAAAC CCTTCAAGTG TCCCGTGTGC 
GATCAGGCCT TCAGGAGGTC CTCCGAGCTC ACCAGTCATA AAAAAGCTCA TTTCGGCCTG 
TTCGGCTACA CCTGCACGGA GTGCGGCAAA CCCTGCAAAA CGCTGACATT GCTGAAGTAT 
CACCAGCGTA CGCACACAGG AGAGAGGCCG TACGTCTGCA AGGAGTGTGG GAAGAGGTTC 
CCCATGCCGA AAGCTTTGCA CAAACACATG ATCTCCCACT CTCCAGAGGG AGCTGAAGGA 
GAAGAAAAAG ACGACGCTGC CAAAAAACGA GCAAAGAAAG ATGAAGGTTC TCCTCCTGTC 
AAACTCCACT GCTCCATGTG CAAGGCCACT TTCAAGAACT CCAAGACTCG CCAGCGGCAC 
ATGAAAATCA AGCACAACGT CCTCCCATCA GGGCAAAGCA AACCTGTTAT CACCCCCATT 
TCGATCTCAC AGCCCACGCG GTTGCAGCTA GAGGCCGCTG GACCTCTGCA AAAAGTTGAT 
GCCAACATCG ACACAGCGCA AATACGCAAA CTTATAGAGT CGCTGGGCAC TGTGAAGAAA 
GTGAACCAAG TGGTTCTGTT GGGTCAGGTG CCGGGTTACG CTCCACCACT GGAATTGCAG 
GCGGATTCGC AGCCAGCAGG GCCTCTGGAT CCCGATCTAA GTCCACCGCA GATCGATTTT 
GGAGGACTGA AGCCGCCCGA ATCCAAGACG ACTGGACTGG ACACTTCCAA TTATCCGTGT 
GGGTTCATGG ACCAAACCAT CATTCTGGAA CCCATCACTC CTGATGGAGA AACCCCCCCC 
CTGTCAGAGC TGGCATCCTG TGTAGCAGCA GGCGAAACTG CACAGACTGT GGGAGAGGTG 
GTTCAGAGGC CCCTGACCAG TGAAATTCTC TTGGATAATA TGACCTGCCA GAACGAGGAG 
AATGACCTTG AACAAACATT CATATTAGAA CTCACCCCAG CTTTGACACC AACTGCAGAA 
CTGGAGCACT CCCAGACTGA ACCACAGAAT ATGGCTCCGT CCTCCTCTCT GGTGCTGAAC 
ACGGACCAGG AGGCTGCAGA TCAAACTGCC AAAAAGTCTG CCCTCAATGG GCAAGACGCC 
AGCCTGACAG AACCGCCACT TGTCTCCACA GCCCAGCTGG ATGGAACATC TCCTTCCTTA 
GACCCAGAGC ACAAAGAACT TCCCTCCAAC CCTTTGGATT CACCAGATAA ACCCTCACAA 
GTTGGCCCCA ATGAAGGCGA TGATGCATGT ACGAAGATGA CGGGTCAAGA CCAGTTGAGT 
GAGGGCGAAT TAAAGACACA GGAACACAAT GGAGAGCATT TGTCTGAGAA ATCTGGAATG 
GAAGGTAAGA AGAACCATTC AGAAGTGGAG TCGTCTTCTA AAGCGGCAGT CCCGTCTGAC 
ATGCAGTCAA AACATGTTCC TCAGACCTCA GAACTGGCCC TGAATGTCAT GTCTGCTCAG 
GAGCTGGTGA AAGTGCGCAA AAGAAAGCCG GCCAGGACGT TTTTCTTTCC AGGATACATT 
CAAGATCTGG CCGGGTCCAT TTACAAAGAC GACTTTCTGA TCGACGCCAA ACCAGCCAAG 
CGGCAAAGAA CAAAAAAGTC TCATCTTGTT GTGAAATTCA GTCCTCAGAG CAAAGACAAG 
AAAAATAAGA AAAAAAAGCA GGAACCGCAG CAGCCAAGAC CAGAAGATCT GACTAAAGGT 
AAAGCTCCGG CTAAGAGAAC ACCTGAGAAA AAGATGTCCT TGTCACCAAA GAAAGGAGCA 
AAGGGAAAAA AGGATGCTGC AGGGGGGGGC GGAGCATCAG GAGTTGTCAC AAAGTCCCCT 
CCAACCCCAG TTGCACAGGA GAAACAAATC AAAGAAGATG TGGGTAAAAA CAAACTAAAA 
AAAAAAAAAG TAGGGAACGA AGATGCACAA ACGAGTGGGC AGAAGGCCGC AGCTTCACCT 
GTGTTCAAAA AGAAAAAGCA ACCAAAGGTT AGACAAGAAG TTTCACCCAA AAGTGCAAAA 
ACTGAAAGAG CTAGGAAAAA AACACCAACC AAAAAAGTCA AAAAGCCAGA AGAACCGAAT 
TTAAACGCAG TACAAGACTC TCTGCTTTTA TTAAAAGGCC ATAAGCAGCC CCAGCTCAAA 
GTTTACAAGC TGGACCCTTC CAGGGCGTCG AGTCAGCCTC AGGACGCCTC CGTCTCCAAG 
AAAAAGGGAG GACGGGCAAA GAAAGCTCTG TCCCTCATGT CTTCCTTGAA GTCTTCTCAA 
CCTCCACCCG ATGCTCCGCC CAACAAGCCA AAGGCCACCA GAAAACGCAA AGCCTCGTCG 
AAAGTGGAAA CTGAAGGAGT CATAACCTCT AAGCGAGCCT TGGAGTGCAA AGACTGTGGG 
GAAAAGTTCA GTGACGTCTC ATCGCTTCAG AAGCACAAAA CGACCGCACA CACCGTAGAG 
AGTCCAAGTC TCACCTACAC CAACGGAAAC ATATTTGAGG GCGTCTCCAG GTTGGAGTTT 
TACCCGTCCC CGAAGGTGTA TGACGGCGGA GCCGCGGGAA AGAATGCAGC AACAGGGTGG 
GACACTGAGC CAGAGATGGG AGAGATGACT GTGGAGGACA GGGAGCAGAA TGTCTCTTTC 
CCAGCTCTGA TTCCATCCCC ATCTCTGCCG GTTTCTCCTC CAAATGTTGA CATAAATGCC 
TTCCAGGGGG CAAGTAGAAG AGACGAGAAC AAAGCCTCCC CTTTAGATGT TTGCTCTCGG 
CCCCGTGACA TGACTGCTGG CAAGGATCGC ACAGATTTCC TAGAGTCCGC TCAGGCGAAG 
TCCTCTGAGA GGAGGCCGCT GTTTCCACCA GAATGTGCCT CAAAGAGCCC CAACTCTGAA 
TGTGAAGCCC AGGGAACCAC CGAGGAAGAC TTCAAGGAAG ATCTACTTCT AGAGGTTGAC 
TTGGTTACTG TTGGGGAACA GAATGAGAGA GACAGAAGTT CTCTGGATGA AGACACTTCT 
ACCCAAAAAG ATTTAAACAC CAGTGATTCT GAAAGTGATA AACACCCCGA GCAGATTTGT 
GATGGAAGGA GTGAAAATGA GGTAACTTCA AAGACTGTGT TTTGCTCAAC CAATCAGGTG 
AAAGAAGAAG AGGAAGAGAT GTTGGTGCAG AGGAGAGGAG AGGACCAGAA GAGCGTTGTG 
ATGAGTAGAC GTAGGAGAAG AGGCTCAGAG CCCAGAAGAG GAGATGCCAT AAGAAAGACG 
GGATCTGTTG GAGAGTCTCA GACGGAAACT GAAAGTGAGC AGGATGAATG TCAGGTGGTT 
TATGAGATAC ACTCCGTCTT CTCGGACCAG GAGTCTGACA CGAAGACCAG CCCTGGATTC 
AGGACCATGA AAGGCCCTGC TTCTGTTGCG TTCATCCCTT CTGGTCCTTC AGCGTTTGAC 
AAATCCTCTG AAGAGCGTGT AGTGCTGGAG CTGGAGTCTG TAACCACTAG TGTTCAGGAA 
GTGATGAACG AACACAGGCT GCATGGAGAC GGGGAGACGG ACAGGGAAGC AGACCAGTCT 
CCAGGCATCG TGCTGGAGAG GTTCCTGACC TCCAGACAAA GATCTTCTGA CAAAGAGGCC 
TCCTGCAGGG CTGCAGACCA ACGAGAGGGT TTTGATGGTG TTTCTGAGAA AGAAGTACAG 
TTTGTTGGGA GCCAGGAGAT CAAAGTTGAA GAGACCCCGC CGGACCCCAC ACTGCAGCCC 
CCTGCCTGTC AAAGCAGGCA GAGGGCCGGT CTCCATCCAC AGCATAATCG AGCCATACGG 
ACCGTTCTAG TGAAAGAGGA GAGCGGCCCC CTGCTGAACG CTGCTCTGGA CGCCCAAGGC 
AGCAGACGGA TGCAGTGGGC AGTGGAGCCA ATCAGCAGCG GGGGGGCCGA TGGGCCGTTA 
ATTGAGAATG GGCAGAGCCC CAATGAGGCA CGCAGGAGTC CAGAGTTCAA CACCAAGCAG 
TGCATCTTCT ATCCAGTGAA GGAGGAGGAG CGGGAACTTC TGCTGGCATC TGCTCAGACC 
AACGCTGACA CCTTCAGCCT GGAAGGCTTC GGTGATGGAG CCCCAGCTGA GCATCAACCT 
GCAGAGTGTG ATGCATATGG AGGAAGACTC TCTGCTGCAG GCTACCAGAA TGGAAGCCTG 
CTGTCAGAAC CAGAGACTTC AGACTTCACT GACAGTCAAG CTTTCTCTGA CTCGGAGTGG 
CAGCCGTCAG ATGTGCGGGA CTTTCTACTC AAGAGTTGTG ATGACGACGT GAGCGGCTTC 
GAACTGCCTG ATCCTCAGCG TGACACAGAA GCAGAAGTGA TGGCTTACTT CAACAGGAAG 
CAGTCCAGCA GCTCGCATGA GCCCAATCAC ACATCCGCGA GTTTTTCAAC ATCGAGTCAT 
CTGCAACCCC AGATGAATGG AACCAGGACC AGAGAGCCCA TTGATTACTT TTCCAAATAT 
TTTGGTTGGG ATACTTGGGC AGAAACTTCA ACTTGCACAA ACAAACTCTG CAAAATGTCA 
GATCCTGTCA CGTCTGTAGA CGTTGCACGG TTTGTTGGTA TCCACATCGC CATGGGAACT 
TTGAAGTTTC CCAGTCCAAG GCTTTACTGG GAGGACTTGA CGAAGGTGCC CCTGATTGCT 
GACGCCATGC CCCTGACTCG CTTTGTGGAG CTGTCCCGTA CGTTACAGCT ACCTTCTCCT 
GCTGAAGAGC AGCCACCTGG TGATGGACAG TCCAGAGATG ACGAGGGGAG GGACTTGAAC 
TGTTCACCAA CACAGATGGA TCCTCTGTGG AAGGTCCAGC CAATCCTGGG CCGCTTCAAT 
GCTGAGTGCC AGTCGTTGAG AAAGGAGGAC AACTATGCCG TTGACCAGTA TCCACTTCCT 
CTGACCTGCA AGACTCGCGA AAGTGGGCCG TCTCTGCTCT GCACTGCACT GATTGGACTT 
GGTGGTTTAC TCTTGCATGT GGATCTCAAT GTGGATCCCC CAGATAAAGA AGGTGTCGTA 
GAAAAGATGG CGCCTAAAAG TAGCATGGTG TTTCTTTGCA AACAGGAGCT TTCCACCCCT 
GCCATGATGG AACGCCTTTT AGATGCTGGG GTTCACGCGG CCGGTAAGGT GGGAGGAGCT 
CGAGGACAAA TCGGAGATGA GTTTGTGAGC TCCGACGGGA AGCTGATGCT CCGCAGATCC 
CACAGCGGCT TCATACTTTC TACAGCGGGG AACGGTCGGA GGAGCATGGC TTCCCTCACC 
AGCAGCTTCC AGAAGGCCCA GATGTCGGCT CGCCTCAACA GAGATTTGCT AAACCTGTAC 
TCCACACCTC TCACGGCCAC GCCTCCACAC AGCTGGCCCC AAGCGGTGCT CTGGTACCTC 
ATCGATTTGG CTTTGGTGAA CTCCTGGCTT TTGTACCGAG AAGATCACGG GGCAGCTGCT 
GGAGCTTTGA CTTTCATGGC TTTCAGGCTG GATGTTTCCA AGGCGCTGAC CCTCTCCGGC 
AGCTCAGACA CACAGGACTC CATTCCTCCT CAGCCTCCAT CAGAGGAAAC CCACGGGACA 
AGCCAAACGC CCAGTCCCAT GCTGGTGGAG GACAGCCCCC TGCCCGACGC CGCCACCCGC 
TACGACGGCG CAGGCCACTG GCCGGAGCAG CTGGCAGAGG GGGAAGGGGG CAGGTGTCGT 
TTCGGGGACT GTCAGAGGAC GTCGCGAGTT TTGTGTCTGA AGTGTTGCGT CTTTCTCTGC 
ATCTCACGAA ATCACAACTG CTTTTTGAAT TTTCATAACC AGGACAATAT AGGCAGAAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020557509.1
CDS	81..6383
Translation	MQSPSLTRTDSIPASSSASEQPVDMVAEPEQQGPEGQAGDHPAVAPADPQPPETSQHTQGGAQSPANVAAMPTAEAEDDAKRSTESSSGAKTNVQTVRRYVPSKKAMIDPLKMDMTKSLVEPLSSSQLSLHCVECHIIFSDHKSKDRHVKLKHPVEYEKRNLKNALFACYVCDRHFTNSTDLMAHQKAHTEKKPFKCPVCDQAFRRSSELTSHKKAHFGLFGYTCTECGKPCKTLTLLKYHQRTHTGERPYVCKECGKRFPMPKALHKHMISHSPEGAEGEEKDDAAKKRAKKDEGSPPVKLHCSMCKATFKNSKTRQRHMKIKHNVLPSGQSKPVITPISISQPTRLQLEAAGPLQKVDANIDTAQIRKLIESLGTVKKVNQVVLLGQVPGYAPPLELQADSQPAGPLDPDLSPPQIDFGGLKPPESKTTGLDTSNYPCGFMDQTIILEPITPDGETPPLSELASCVAAGETAQTVGEVVQRPLTSEILLDNMTCQNEENDLEQTFILELTPALTPTAELEHSQTEPQNMAPSSSLVLNTDQEAADQTAKKSALNGQDASLTEPPLVSTAQLDGTSPSLDPEHKELPSNPLDSPDKPSQVGPNEGDDACTKMTGQDQLSEGELKTQEHNGEHLSEKSGMEGKKNHSEVESSSKAAVPSDMQSKHVPQTSELALNVMSAQELVKVRKRKPARTFFFPGYIQDLAGSIYKDDFLIDAKPAKRQRTKKSHLVVKFSPQSKDKKNKKKKQEPQQPRPEDLTKGKAPAKRTPEKKMSLSPKKGAKGKKDAAGGGGASGVVTKSPPTPVAQEKQIKEDVGKNKLKKKKVGNEDAQTSGQKAAASPVFKKKKQPKVRQEVSPKSAKTERARKKTPTKKVKKPEEPNLNAVQDSLLLLKGHKQPQLKVYKLDPSRASSQPQDASVSKKKGGRAKKALSLMSSLKSSQPPPDAPPNKPKATRKRKASSKVETEGVITSKRALECKDCGEKFSDVSSLQKHKTTAHTVESPSLTYTNGNIFEGVSRLEFYPSPKVYDGGAAGKNAATGWDTEPEMGEMTVEDREQNVSFPALIPSPSLPVSPPNVDINAFQGASRRDENKASPLDVCSRPRDMTAGKDRTDFLESAQAKSSERRPLFPPECASKSPNSECEAQGTTEEDFKEDLLLEVDLVTVGEQNERDRSSLDEDTSTQKDLNTSDSESDKHPEQICDGRSENEVTSKTVFCSTNQVKEEEEEMLVQRRGEDQKSVVMSRRRRRGSEPRRGDAIRKTGSVGESQTETESEQDECQVVYEIHSVFSDQESDTKTSPGFRTMKGPASVAFIPSGPSAFDKSSEERVVLELESVTTSVQEVMNEHRLHGDGETDREADQSPGIVLERFLTSRQRSSDKEASCRAADQREGFDGVSEKEVQFVGSQEIKVEETPPDPTLQPPACQSRQRAGLHPQHNRAIRTVLVKEESGPLLNAALDAQGSRRMQWAVEPISSGGADGPLIENGQSPNEARRSPEFNTKQCIFYPVKEEERELLLASAQTNADTFSLEGFGDGAPAEHQPAECDAYGGRLSAAGYQNGSLLSEPETSDFTDSQAFSDSEWQPSDVRDFLLKSCDDDVSGFELPDPQRDTEAEVMAYFNRKQSSSSHEPNHTSASFSTSSHLQPQMNGTRTREPIDYFSKYFGWDTWAETSTCTNKLCKMSDPVTSVDVARFVGIHIAMGTLKFPSPRLYWEDLTKVPLIADAMPLTRFVELSRTLQLPSPAEEQPPGDGQSRDDEGRDLNCSPTQMDPLWKVQPILGRFNAECQSLRKEDNYAVDQYPLPLTCKTRESGPSLLCTALIGLGGLLLHVDLNVDPPDKEGVVEKMAPKSSMVFLCKQELSTPAMMERLLDAGVHAAGKVGGARGQIGDEFVSSDGKLMLRRSHSGFILSTAGNGRRSMASLTSSFQKAQMSARLNRDLLNLYSTPLTATPPHSWPQAVLWYLIDLALVNSWLLYREDHGAAAGALTFMAFRLDVSKALTLSGSSDTQDSIPPQPPSEETHGTSQTPSPMLVEDSPLPDAATRYDGAGHWPEQLAEGEGGRCRFGDCQRTSRVLCLKCCVFLCISRNHNCFLNFHNQDNIGRK

Target ORF information:

RefSeq Version	XM_020701850.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes uncharacterized LOC101162913 (LOC101162913), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020701850.2

ORF Insert Sequence:

ATGCAGAGTC CATCTTTGAC CCGGACGGAC TCCATCCCTG CCTCCTCATC GGCCTCAGAG 
CAGCCCGTGG ACATGGTGGC TGAGCCGGAG CAGCAGGGAC CAGAGGGACA GGCGGGGGAC 
CACCCGGCAG TGGCTCCTGC AGACCCCCAA CCACCAGAGA CGAGTCAACA CACCCAAGGG 
GGAGCACAGA GCCCTGCAAA TGTAGCGGCC ATGCCAACAG CAGAGGCTGA AGATGATGCC 
AAGAGAAGCA CTGAGAGCAG CAGTGGAGCG AAGACAAACG TGCAGACCGT CAGGAGATAC 
GTCCCTTCCA AGAAAGCAAT GATAGATCCT CTGAAGATGG ATATGACCAA ATCGCTGGTT 
GAGCCACTCT CATCCTCTCA GCTCTCTCTG CACTGCGTCG AGTGCCACAT CATCTTCAGT 
GACCACAAGA GCAAAGACCG CCACGTGAAG CTGAAACACC CAGTAGAATA TGAAAAGCGC 
AACCTGAAAA ACGCCCTTTT TGCATGTTAC GTGTGTGACC GCCATTTTAC CAACTCCACG 
GACCTCATGG CCCACCAGAA GGCCCACACT GAGAAGAAAC CCTTCAAGTG TCCCGTGTGC 
GATCAGGCCT TCAGGAGGTC CTCCGAGCTC ACCAGTCATA AAAAAGCTCA TTTCGGCCTG 
TTCGGCTACA CCTGCACGGA GTGCGGCAAA CCCTGCAAAA CGCTGACATT GCTGAAGTAT 
CACCAGCGTA CGCACACAGG AGAGAGGCCG TACGTCTGCA AGGAGTGTGG GAAGAGGTTC 
CCCATGCCGA AAGCTTTGCA CAAACACATG ATCTCCCACT CTCCAGAGGG AGCTGAAGGA 
GAAGAAAAAG ACGACGCTGC CAAAAAACGA GCAAAGAAAG ATGAAGGTTC TCCTCCTGTC 
AAACTCCACT GCTCCATGTG CAAGGCCACT TTCAAGAACT CCAAGACTCG CCAGCGGCAC 
ATGAAAATCA AGCACAACGT CCTCCCATCA GGGCAAAGCA AACCTGTTAT CACCCCCATT 
TCGATCTCAC AGCCCACGCG GTTGCAGCTA GAGGCCGCTG GACCTCTGCA AAAAGTTGAT 
GCCAACATCG ACACAGCGCA AATACGCAAA CTTATAGAGT CGCTGGGCAC TGTGAAGAAA 
GTGAACCAAG TGGTTCTGTT GGGTCAGGTG CCGGGTTACG CTCCACCACT GGAATTGCAG 
GCGGATTCGC AGCCAGCAGG GCCTCTGGAT CCCGATCTAA GTCCACCGCA GATCGATTTT 
GGAGGACTGA AGCCGCCCGA ATCCAAGACG ACTGGACTGG ACACTTCCAA TTATCCGTGT 
GGGTTCATGG ACCAAACCAT CATTCTGGAA CCCATCACTC CTGATGGAGA AACCCCCCCC 
CTGTCAGAGC TGGCATCCTG TGTAGCAGCA GGCGAAACTG CACAGACTGT GGGAGAGGTG 
GTTCAGAGGC CCCTGACCAG TGAAATTCTC TTGGATAATA TGACCTGCCA GAACGAGGAG 
AATGACCTTG AACAAACATT CATATTAGAA CTCACCCCAG CTTTGACACC AACTGCAGAA 
CTGGAGCACT CCCAGACTGA ACCACAGAAT ATGGCTCCGT CCTCCTCTCT GGTGCTGAAC 
ACGGACCAGG AGGCTGCAGA TCAAACTGCC AAAAAGTCTG CCCTCAATGG GCAAGACGCC 
AGCCTGACAG AACCGCCACT TGTCTCCACA GCCCAGCTGG ATGGAACATC TCCTTCCTTA 
GACCCAGAGC ACAAAGAACT TCCCTCCAAC CCTTTGGATT CACCAGATAA ACCCTCACAA 
GTTGGCCCCA ATGAAGGCGA TGATGCATGT ACGAAGATGA CGGGTCAAGA CCAGTTGAGT 
GAGGGCGAAT TAAAGACACA GGAACACAAT GGAGAGCATT TGTCTGAGAA ATCTGGAATG 
GAAGGTAAGA AGAACCATTC AGAAGTGGAG TCGTCTTCTA AAGCGGCAGT CCCGTCTGAC 
ATGCAGTCAA AACATGTTCC TCAGACCTCA GAACTGGCCC TGAATGTCAT GTCTGCTCAG 
GAGCTGGTGA AAGTGCGCAA AAGAAAGCCG GCCAGGACGT TTTTCTTTCC AGGATACATT 
CAAGATCTGG CCGGGTCCAT TTACAAAGAC GACTTTCTGA TCGACGCCAA ACCAGCCAAG 
CGGCAAAGAA CAAAAAAGTC TCATCTTGTT GTGAAATTCA GTCCTCAGAG CAAAGACAAG 
AAAAATAAGA AAAAAAAGCA GGAACCGCAG CAGCCAAGAC CAGAAGATCT GACTAAAGGT 
AAAGCTCCGG CTAAGAGAAC ACCTGAGAAA AAGATGTCCT TGTCACCAAA GAAAGGAGCA 
AAGGGAAAAA AGGATGCTGC AGGGGGGGGC GGAGCATCAG GAGTTGTCAC AAAGTCCCCT 
CCAACCCCAG TTGCACAGGA GAAACAAATC AAAGAAGATG TGGGTAAAAA CAAACTAAAA 
AAAAAAAAAG TAGGGAACGA AGATGCACAA ACGAGTGGGC AGAAGGCCGC AGCTTCACCT 
GTGTTCAAAA AGAAAAAGCA ACCAAAGGTT AGACAAGAAG TTTCACCCAA AAGTGCAAAA 
ACTGAAAGAG CTAGGAAAAA AACACCAACC AAAAAAGTCA AAAAGCCAGA AGAACCGAAT 
TTAAACGCAG TACAAGACTC TCTGCTTTTA TTAAAAGGCC ATAAGCAGCC CCAGCTCAAA 
GTTTACAAGC TGGACCCTTC CAGGGCGTCG AGTCAGCCTC AGGACGCCTC CGTCTCCAAG 
AAAAAGGGAG GACGGGCAAA GAAAGCTCTG TCCCTCATGT CTTCCTTGAA GTCTTCTCAA 
CCTCCACCCG ATGCTCCGCC CAACAAGCCA AAGGCCACCA GAAAACGCAA AGCCTCGTCG 
AAAGTGGAAA CTGAAGGAGT CATAACCTCT AAGCGAGCCT TGGAGTGCAA AGACTGTGGG 
GAAAAGTTCA GTGACGTCTC ATCGCTTCAG AAGCACAAAA CGACCGCACA CACCGTAGAG 
AGTCCAAGTC TCACCTACAC CAACGGAAAC ATATTTGAGG GCGTCTCCAG GTTGGAGTTT 
TACCCGTCCC CGAAGGTGTA TGACGGCGGA GCCGCGGGAA AGAATGCAGC AACAGGGTGG 
GACACTGAGC CAGAGATGGG AGAGATGACT GTGGAGGACA GGGAGCAGAA TGTCTCTTTC 
CCAGCTCTGA TTCCATCCCC ATCTCTGCCG GTTTCTCCTC CAAATGTTGA CATAAATGCC 
TTCCAGGGGG CAAGTAGAAG AGACGAGAAC AAAGCCTCCC CTTTAGATGT TTGCTCTCGG 
CCCCGTGACA TGACTGCTGG CAAGGATCGC ACAGATTTCC TAGAGTCCGC TCAGGCGAAG 
TCCTCTGAGA GGAGGCCGCT GTTTCCACCA GAATGTGCCT CAAAGAGCCC CAACTCTGAA 
TGTGAAGCCC AGGGAACCAC CGAGGAAGAC TTCAAGGAAG ATCTACTTCT AGAGGTTGAC 
TTGGTTACTG TTGGGGAACA GAATGAGAGA GACAGAAGTT CTCTGGATGA AGACACTTCT 
ACCCAAAAAG ATTTAAACAC CAGTGATTCT GAAAGTGATA AACACCCCGA GCAGATTTGT 
GATGGAAGGA GTGAAAATGA GGTAACTTCA AAGACTGTGT TTTGCTCAAC CAATCAGGTG 
AAAGAAGAAG AGGAAGAGAT GTTGGTGCAG AGGAGAGGAG AGGACCAGAA GAGCGTTGTG 
ATGAGTAGAC GTAGGAGAAG AGGCTCAGAG CCCAGAAGAG GAGATGCCAT AAGAAAGACG 
GGATCTGTTG GAGAGTCTCA GACGGAAACT GAAAGTGAGC AGGATGAATG TCAGGTGGTT 
TATGAGATAC ACTCCGTCTT CTCGGACCAG GAGTCTGACA CGAAGACCAG CCCTGGATTC 
AGGACCATGA AAGGCCCTGC TTCTGTTGCG TTCATCCCTT CTGGTCCTTC AGCGTTTGAC 
AAATCCTCTG AAGAGCGTGT AGTGCTGGAG CTGGAGTCTG TAACCACTAG TGTTCAGGAA 
GTGATGAACG AACACAGGCT GCATGGAGAC GGGGAGACGG ACAGGGAAGC AGACCAGTCT 
CCAGGCATCG TGCTGGAGAG GTTCCTGACC TCCAGACAAA GATCTTCTGA CAAAGAGGCC 
TCCTGCAGGG CTGCAGACCA ACGAGAGGGT TTTGATGGTG TTTCTGAGAA AGAAGTACAG 
TTTGTTGGGA GCCAGGAGAT CAAAGTTGAA GAGACCCCGC CGGACCCCAC ACTGCAGCCC 
CCTGCCTGTC AAAGCAGGCA GAGGGCCGGT CTCCATCCAC AGCATAATCG AGCCATACGG 
ACCGTTCTAG TGAAAGAGGA GAGCGGCCCC CTGCTGAACG CTGCTCTGGA CGCCCAAGGC 
AGCAGACGGA TGCAGTGGGC AGTGGAGCCA ATCAGCAGCG GGGGGGCCGA TGGGCCGTTA 
ATTGAGAATG GGCAGAGCCC CAATGAGGCA CGCAGGAGTC CAGAGTTCAA CACCAAGCAG 
TGCATCTTCT ATCCAGTGAA GGAGGAGGAG CGGGAACTTC TGCTGGCATC TGCTCAGACC 
AACGCTGACA CCTTCAGCCT GGAAGGCTTC GGTGATGGAG CCCCAGCTGA GCATCAACCT 
GCAGAGTGTG ATGCATATGG AGGAAGACTC TCTGCTGCAG GCTACCAGAA TGGAAGCCTG 
CTGTCAGAAC CAGAGACTTC AGACTTCACT GACAGTCAAG CTTTCTCTGA CTCGGAGTGG 
CAGCCGTCAG ATGTGCGGGA CTTTCTACTC AAGAGTTGTG ATGACGACGT GAGCGGCTTC 
GAACTGCCTG ATCCTCAGCG TGACACAGAA GCAGAAGTGA TGGCTTACTT CAACAGGAAG 
CAGTCCAGCA GCTCGCATGA GCCCAATCAC ACATCCGCGA GTTTTTCAAC ATCGAGTCAT 
CTGCAACCCC AGATGAATGG AACCAGGACC AGAGAGCCCA TTGATTACTT TTCCAAATAT 
TTTGGTTGGG ATACTTGGGC AGAAACTTCA ACTTGCACAA ACAAACTCTG CAAAATGTCA 
GATCCTGTCA CGTCTGTAGA CGTTGCACGG TTTGTTGGTA TCCACATCGC CATGGGAACT 
TTGAAGTTTC CCAGTCCAAG GCTTTACTGG GAGGACTTGA CGAAGGTGCC CCTGATTGCT 
GACGCCATGC CCCTGACTCG CTTTGTGGAG CTGTCCCGTA CGTTACAGCT ACCTTCTCCT 
GCTGAAGAGC AGCCACCTGG TGATGGACAG TCCAGAGATG ACGAGGGGAG GGACTTGAAC 
TGTTCACCAA CACAGATGGA TCCTCTGTGG AAGGTCCAGC CAATCCTGGG CCGCTTCAAT 
GCTGAGTGCC AGTCGTTGAG AAAGGAGGAC AACTATGCCG TTGACCAGTA TCCACTTCCT 
CTGACCTGCA AGACTCGCGA AAGTGGGCCG TCTCTGCTCT GCACTGCACT GATTGGACTT 
GGTGGTTTAC TCTTGCATGT GGATCTCAAT GTGGATCCCC CAGATAAAGA AGGTGTCGTA 
GAAAAGATGG CGCCTAAAAG TAGCATGGTG TTTCTTTGCA AACAGGAGCT TTCCACCCCT 
GCCATGATGG AACGCCTTTT AGATGCTGGG GTTCACGCGG CCGGTAAGGT GGGAGGAGCT 
CGAGGACAAA TCGGAGATGA GTTTGTGAGC TCCGACGGGA AGCTGATGCT CCGCAGATCC 
CACAGCGGCT TCATACTTTC TACAGCGGGG AACGGTCGGA GGAGCATGGC TTCCCTCACC 
AGCAGCTTCC AGAAGGCCCA GATGTCGGCT CGCCTCAACA GAGATTTGCT AAACCTGTAC 
TCCACACCTC TCACGGCCAC GCCTCCACAC AGCTGGCCCC AAGCGGTGCT CTGGTACCTC 
ATCGATTTGG CTTTGGTGAA CTCCTGGCTT TTGTACCGAG AAGATCACGG GGCAGCTGCT 
GGAGCTTTGA CTTTCATGGC TTTCAGGCTG GATGTTTCCA AGGCGCTGAC CCTCTCCGGC 
AGCTCAGACA CACAGGACTC CATTCCTCCT CAGCCTCCAT CAGAGGAAAC CCACGGGACA 
AGCCAAACGC CCAGTCCCAT GCTGGTGGAG GACAGCCCCC TGCCCGACGC CGCCACCCGC 
TACGACGGCG CAGGCCACTG GCCGGAGCAG CTGGCAGAGG GGGAAGGGGG CAGGTGTCGT 
TTCGGGGACT GTCAGAGGAC GTCGCGAGTT TTGTGTCTGA AGTGTTGCGT CTTTCTCTGC 
ATCTCACGAA ATCACAACTG CTTTTTGAAT TTTCATAACC AGGACAATAT AGGCAGAAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101162913 ( XM_023964092.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023819860.1 Oryzias latipes uncharacterized LOC101162913 (LOC101162913), transcript variant X3, mRNA.

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CloneID	OJa27423
Clone ID Related Accession (Same CDS sequence)	XM_023964092.1
Accession Version	XM_023964092.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	uncharacterized protein LOC101162913 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019874.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGTC CATCTTTGAC CCGGACGGAC TCCATCCCTG CCTCCTCATC GGCCTCAGAG 
CAGCCCGTGG ACATGGTGGC TGAGCCGGAG CAGCAGGGAC CAGAGGGACA GGCGGGGGAC 
CACCCGGCAG TGGCTCCTGC AGACCCCCAA CCACCAGAGA CGAGTCAACA CACCCAAGGG 
GGAGCACAGA GCCCTGCAAA TGTAGCGGCC ATGCCAACAG CAGAGGCTGA AGATGATGCC 
AAGAGAAGCA CTGAGAGCAG CAGTGGAGCG AAGACAAACG TGCAGACCGT CAGGAGATAC 
GTCCCTTCCA AGAAAGCAAT GATAGATCCT CTGAAGATGG ATATGACCAA ATCGCTGGTT 
GAGCCACTCT CATCCTCTCA GCTCTCTCTG CACTGCGTCG AGTGCCACAT CATCTTCAGT 
GACCACAAGA GCAAAGACCG CCACGTGAAG CTGAAACACC CAGTAGAATA TGAAAAGCGC 
AACCTGAAAA ACGCCCTTTT TGCATGTTAC GTGTGTGACC GCCATTTTAC CAACTCCACG 
GACCTCATGG CCCACCAGAA GGCCCACACT GAGAAGAAAC CCTTCAAGTG TCCCGTGTGC 
GATCAGGCCT TCAGGAGGTC CTCCGAGCTC ACCAGTCATA AAAAAGCTCA TTTCGGCCTG 
TTCGGCTACA CCTGCACGGA GTGCGGCAAA CCCTGCAAAA CGCTGACATT GCTGAAGTAT 
CACCAGCGTA CGCACACAGG AGAGAGGCCG TACGTCTGCA AGGAGTGTGG GAAGAGGTTC 
CCCATGCCGA AAGCTTTGCA CAAACACATG ATCTCCCACT CTCCAGAGGG AGCTGAAGGA 
GAAGAAAAAG ACGACGCTGC CAAAAAACGA GCAAAGAAAG ATGAAGGTTC TCCTCCTGTC 
AAACTCCACT GCTCCATGTG CAAGGCCACT TTCAAGAACT CCAAGACTCG CCAGCGGCAC 
ATGAAAATCA AGCACAACGT CCTCCCATCA GGGCAAAGCA AACCTGTTAT CACCCCCATT 
TCGATCTCAC AGCCCACGCG GTTGCAGCTA GAGGCCGCTG GACCTCTGCA AAAAGTTGAT 
GCCAACATCG ACACAGCGCA AATACGCAAA CTTATAGAGT CGCTGGGCAC TGTGAAGAAA 
GTGAACCAAG TGGTTCTGTT GGGTCAGGTG CCGGGTTACG CTCCACCACT GGAATTGCAG 
GCGGATTCGC AGCCAGCAGG GCCTCTGGAT CCCGATCTAA GTCCACCGCA GATCGATTTT 
GGAGGACTGA AGCCGCCCGA ATCCAAGACG ACTGGACTGG ACACTTCCAA TTATCCGTGT 
GGGTTCATGG ACCAAACCAT CATTCTGGAA CCCATCACTC CTGATGGAGA AACCCCCCCC 
CTGTCAGAGC TGGCATCCTG TGTAGCAGCA GGCGAAACTG CACAGACTGT GGGAGAGGTG 
GTTCAGAGGC CCCTGACCAG TGAAATTCTC TTGGATAATA TGACCTGCCA GAACGAGGAG 
AATGACCTTG AACAAACATT CATATTAGAA CTCACCCCAG CTTTGACACC AACTGCAGAA 
CTGGAGCACT CCCAGACTGA ACCACAGAAT ATGGCTCCGT CCTCCTCTCT GGTGCTGAAC 
ACGGACCAGG AGGCTGCAGA TCAAACTGCC AAAAAGTCTG CCCTCAATGG GCAAGACGCC 
AGCCTGACAG AACCGCCACT TGTCTCCACA GCCCAGCTGG ATGGAACATC TCCTTCCTTA 
GACCCAGAGC ACAAAGAACT TCCCTCCAAC CCTTTGGATT CACCAGATAA ACCCTCACAA 
GTTGGCCCCA ATGAAGGCGA TGATGCATGT ACGAAGATGA CGGGTCAAGA CCAGTTGAGT 
GAGGGCGAAT TAAAGACACA GGAACACAAT GGAGAGCATT TGTCTGAGAA ATCTGGAATG 
GAAGGTAAGA AGAACCATTC AGAAGTGGAG TCGTCTTCTA AAGCGGCAGT CCCGTCTGAC 
ATGCAGTCAA AACATGTTCC TCAGACCTCA GAACTGGCCC TGAATGTCAT GTCTGCTCAG 
GAGCTGGTGA AAGTGCGCAA AAGAAAGCCG GCCAGGACGT TTTTCTTTCC AGGATACATT 
CAAGATCTGG CCGGGTCCAT TTACAAAGAC GACTTTCTGA TCGACGCCAA ACCAGCCAAG 
CGGCAAAGAA CAAAAAAGTC TCATCTTGTT GTGAAATTCA GTCCTCAGAG CAAAGACAAG 
AAAAATAAGA AAAAAAAGCA GGAACCGCAG CAGCCAAGAC CAGAAGATCT GACTAAAGGT 
AAAGCTCCGG CTAAGAGAAC ACCTGAGAAA AAGATGTCCT TGTCACCAAA GAAAGGAGCA 
AAGGGAAAAA AGGATGCTGC AGGGGGGGGC GGAGCATCAG GAGTTGTCAC AAAGTCCCCT 
CCAACCCCAG TTGCACAGGA GAAACAAATC AAAGAAGATG TGGGTAAAAA CAAACTAAAA 
AAAAAAAAAG TAGGGAACGA AGATGCACAA ACGAGTGGGC AGAAGGCCGC AGCTTCACCT 
GTGTTCAAAA AGAAAAAGCA ACCAAAGGTT AGACAAGAAG TTTCACCCAA AAGTGCAAAA 
ACTGAAAGAG CTAGGAAAAA AACACCAACC AAAAAAGTCA AAAAGCCAGA AGAACCGAAT 
TTAAACGCAG TACAAGACTC TCTGCTTTTA TTAAAAGGCC ATAAGCAGCC CCAGCTCAAA 
GTTTACAAGC TGGACCCTTC CAGGGCGTCG AGTCAGCCTC AGGACGCCTC CGTCTCCAAG 
AAAAAGGGAG GACGGGCAAA GAAAGCTCTG TCCCTCATGT CTTCCTTGAA GTCTTCTCAA 
CCTCCACCCG ATGCTCCGCC CAACAAGCCA AAGGCCACCA GAAAACGCAA AGCCTCGTCG 
AAAGTGGAAA CTGAAGGAGT CATAACCTCT AAGCGAGCCT TGGAGTGCAA AGACTGTGGG 
GAAAAGTTCA GTGACGTCTC ATCGCTTCAG AAGCACAAAA CGACCGCACA CACCGTAGAG 
AGTCCAAGTC TCACCTACAC CAACGGAAAC ATATTTGAGG GCGTCTCCAG GTTGGAGTTT 
TACCCGTCCC CGAAGGTGTA TGACGGCGGA GCCGCGGGAA AGAATGCAGC AACAGGGTGG 
GACACTGAGC CAGAGATGGG AGAGATGACT GTGGAGGACA GGGAGCAGAA TGTCTCTTTC 
CCAGCTCTGA TTCCATCCCC ATCTCTGCCG GTTTCTCCTC CAAATGTTGA CATAAATGCC 
TTCCAGGGGG CAAGTAGAAG AGACGAGAAC AAAGCCTCCC CTTTAGATGT TTGCTCTCGG 
CCCCGTGACA TGACTGCTGG CAAGGATCGC ACAGATTTCC TAGAGTCCGC TCAGGCGAAG 
TCCTCTGAGA GGAGGCCGCT GTTTCCACCA GAATGTGCCT CAAAGAGCCC CAACTCTGAA 
TGTGAAGCCC AGGGAACCAC CGAGGAAGAC TTCAAGGAAG ATCTACTTCT AGAGGTTGAC 
TTGGTTACTG TTGGGGAACA GAATGAGAGA GACAGAAGTT CTCTGGATGA AGACACTTCT 
ACCCAAAAAG ATTTAAACAC CAGTGATTCT GAAAGTGATA AACACCCCGA GCAGATTTGT 
GATGGAAGGA GTGAAAATGA GGTAACTTCA AAGACTGTGT TTTGCTCAAC CAATCAGGTG 
AAAGAAGAAG AGGAAGAGAT GTTGGTGCAG AGGAGAGGAG AGGACCAGAA GAGCGTTGTG 
ATGAGTAGAC GTAGGAGAAG AGGCTCAGAG CCCAGAAGAG GAGATGCCAT AAGAAAGACG 
GGATCTGTTG GAGAGTCTCA GACGGAAACT GAAAGTGAGC AGGATGAATG TCAGGTGGTT 
TATGAGATAC ACTCCGTCTT CTCGGACCAG GAGTCTGACA CGAAGACCAG CCCTGGATTC 
AGGACCATGA AAGGCCCTGC TTCTGTTGCG TTCATCCCTT CTGGTCCTTC AGCGTTTGAC 
AAATCCTCTG AAGAGCGTGT AGTGCTGGAG CTGGAGTCTG TAACCACTAG TGTTCAGGAA 
GTGATGAACG AACACAGGCT GCATGGAGAC GGGGAGACGG ACAGGGAAGC AGACCAGTCT 
CCAGGCATCG TGCTGGAGAG GTTCCTGACC TCCAGACAAA GATCTTCTGA CAAAGAGGCC 
TCCTGCAGGG CTGCAGACCA ACGAGAGGGT TTTGATGGTG TTTCTGAGAA AGAAGTACAG 
TTTGTTGGGA GCCAGGAGAT CAAAGTTGAA GAGACCCCGC CGGACCCCAC ACTGCAGCCC 
CCTGCCTGTC AAAGCAGGCA GAGGGCCGGT CTCCATCCAC AGCATAATCG AGCCATACGG 
ACCGTTCTAG TGAAAGAGGA GAGCGGCCCC CTGCTGAACG CTGCTCTGGA CGCCCAAGGC 
AGCAGACGGA TGCAGTGGGC AGTGGAGCCA ATCAGCAGCG GGGGGGCCGA TGGGCCGTTA 
ATTGAGAATG GGCAGAGCCC CAATGAGGCA CGCAGGAGTC CAGAGTTCAA CACCAAGCAG 
TGCATCTTCT ATCCAGTGAA GGAGGAGGAG CGGGAACTTC TGCTGGCATC TGCTCAGACC 
AACGCTGACA CCTTCAGCCT GGAAGGCTTC GGTGATGGAG CCCCAGCTGA GCATCAACCT 
GCAGAGTGTG ATGCATCTTT CTCTGACTCG GAGTGGCAGC CGTCAGATGT GCGGGACTTT 
CTACTCAAGA GTTGTGATGA CGACGTGAGC GGCTTCGAAC TGCCTGATCC TCAGCGTGAC 
ACAGAAGCAG AAGTGATGGC TTACTTCAAC AGGAAGCAGT CCAGCAGCTC GCATGAGCCC 
AATCACACAT CCGCGAGTTT TTCAACATCG AGTCATCTGC AACCCCAGAT GAATGGAACC 
AGGACCAGAG AGCCCATTGA TTACTTTTCC AAATATTTTG GTTGGGATAC TTGGGCAGAA 
ACTTCAACTT GCACAAACAA ACTCTGCAAA ATGTCAGATC CTGTCACGTC TGTAGACGTT 
GCACGGTTTG TTGGTATCCA CATCGCCATG GGAACTTTGA AGTTTCCCAG TCCAAGGCTT 
TACTGGGAGG ACTTGACGAA GGTGCCCCTG ATTGCTGACG CCATGCCCCT GACTCGCTTT 
GTGGAGCTGT CCCGTACGTT ACAGCTACCT TCTCCTGCTG AAGAGCAGCC ACCTGGTGAT 
GGACAGTCCA GAGATGACGA GGGGAGGGAC TTGAACTGTT CACCAACACA GATGGATCCT 
CTGTGGAAGG TCCAGCCAAT CCTGGGCCGC TTCAATGCTG AGTGCCAGTC GTTGAGAAAG 
GAGGACAACT ATGCCGTTGA CCAGTATCCA CTTCCTCTGA CCTGCAAGAC TCGCGAAAGT 
GGGCCGTCTC TGCTCTGCAC TGCACTGATT GGACTTGGTG GTTTACTCTT GCATGTGGAT 
CTCAATGTGG ATCCCCCAGA TAAAGAAGGT GTCGTAGAAA AGATGGCGCC TAAAAGTAGC 
ATGGTGTTTC TTTGCAAACA GGAGCTTTCC ACCCCTGCCA TGATGGAACG CCTTTTAGAT 
GCTGGGGTTC ACGCGGCCGG TAAGGTGGGA GGAGCTCGAG GACAAATCGG AGATGAGTTT 
GTGAGCTCCG ACGGGAAGCT GATGCTCCGC AGATCCCACA GCGGCTTCAT ACTTTCTACA 
GCGGGGAACG GTCGGAGGAG CATGGCTTCC CTCACCAGCA GCTTCCAGAA GGCCCAGATG 
TCGGCTCGCC TCAACAGAGA TTTGCTAAAC CTGTACTCCA CACCTCTCAC GGCCACGCCT 
CCACACAGCT GGCCCCAAGC GGTGCTCTGG TACCTCATCG ATTTGGCTTT GGTGAACTCC 
TGGCTTTTGT ACCGAGAAGA TCACGGGGCA GCTGCTGGAG CTTTGACTTT CATGGCTTTC 
AGGCTGGATG TTTCCAAGGC GCTGACCCTC TCCGGCAGCT CAGACACACA GGACTCCATT 
CCTCCTCAGC CTCCATCAGA GGAAACCCAC GGGACAAGCC AAACGCCCAG TCCCATGCTG 
GTGGAGGACA GCCCCCTGCC CGACGCCGCC ACCCGCTACG ACGGCGCAGG CCACTGGCCG 
GAGCAGCTGG CAGAGGGGGA AGGGGGCAGG TGTCGTTTCG GGGACTGTCA GAGGACGTCG 
CGAGTTTTGT GTCTGAAGTG TTGCGTCTTT CTCTGCATCT CACGAAATCA CAACTGCTTT 
TTGAATTTTC ATAACCAGGA CAATATAGGC AGAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023819860.1
CDS	127..6345
Translation	MQSPSLTRTDSIPASSSASEQPVDMVAEPEQQGPEGQAGDHPAVAPADPQPPETSQHTQGGAQSPANVAAMPTAEAEDDAKRSTESSSGAKTNVQTVRRYVPSKKAMIDPLKMDMTKSLVEPLSSSQLSLHCVECHIIFSDHKSKDRHVKLKHPVEYEKRNLKNALFACYVCDRHFTNSTDLMAHQKAHTEKKPFKCPVCDQAFRRSSELTSHKKAHFGLFGYTCTECGKPCKTLTLLKYHQRTHTGERPYVCKECGKRFPMPKALHKHMISHSPEGAEGEEKDDAAKKRAKKDEGSPPVKLHCSMCKATFKNSKTRQRHMKIKHNVLPSGQSKPVITPISISQPTRLQLEAAGPLQKVDANIDTAQIRKLIESLGTVKKVNQVVLLGQVPGYAPPLELQADSQPAGPLDPDLSPPQIDFGGLKPPESKTTGLDTSNYPCGFMDQTIILEPITPDGETPPLSELASCVAAGETAQTVGEVVQRPLTSEILLDNMTCQNEENDLEQTFILELTPALTPTAELEHSQTEPQNMAPSSSLVLNTDQEAADQTAKKSALNGQDASLTEPPLVSTAQLDGTSPSLDPEHKELPSNPLDSPDKPSQVGPNEGDDACTKMTGQDQLSEGELKTQEHNGEHLSEKSGMEGKKNHSEVESSSKAAVPSDMQSKHVPQTSELALNVMSAQELVKVRKRKPARTFFFPGYIQDLAGSIYKDDFLIDAKPAKRQRTKKSHLVVKFSPQSKDKKNKKKKQEPQQPRPEDLTKGKAPAKRTPEKKMSLSPKKGAKGKKDAAGGGGASGVVTKSPPTPVAQEKQIKEDVGKNKLKKKKVGNEDAQTSGQKAAASPVFKKKKQPKVRQEVSPKSAKTERARKKTPTKKVKKPEEPNLNAVQDSLLLLKGHKQPQLKVYKLDPSRASSQPQDASVSKKKGGRAKKALSLMSSLKSSQPPPDAPPNKPKATRKRKASSKVETEGVITSKRALECKDCGEKFSDVSSLQKHKTTAHTVESPSLTYTNGNIFEGVSRLEFYPSPKVYDGGAAGKNAATGWDTEPEMGEMTVEDREQNVSFPALIPSPSLPVSPPNVDINAFQGASRRDENKASPLDVCSRPRDMTAGKDRTDFLESAQAKSSERRPLFPPECASKSPNSECEAQGTTEEDFKEDLLLEVDLVTVGEQNERDRSSLDEDTSTQKDLNTSDSESDKHPEQICDGRSENEVTSKTVFCSTNQVKEEEEEMLVQRRGEDQKSVVMSRRRRRGSEPRRGDAIRKTGSVGESQTETESEQDECQVVYEIHSVFSDQESDTKTSPGFRTMKGPASVAFIPSGPSAFDKSSEERVVLELESVTTSVQEVMNEHRLHGDGETDREADQSPGIVLERFLTSRQRSSDKEASCRAADQREGFDGVSEKEVQFVGSQEIKVEETPPDPTLQPPACQSRQRAGLHPQHNRAIRTVLVKEESGPLLNAALDAQGSRRMQWAVEPISSGGADGPLIENGQSPNEARRSPEFNTKQCIFYPVKEEERELLLASAQTNADTFSLEGFGDGAPAEHQPAECDASFSDSEWQPSDVRDFLLKSCDDDVSGFELPDPQRDTEAEVMAYFNRKQSSSSHEPNHTSASFSTSSHLQPQMNGTRTREPIDYFSKYFGWDTWAETSTCTNKLCKMSDPVTSVDVARFVGIHIAMGTLKFPSPRLYWEDLTKVPLIADAMPLTRFVELSRTLQLPSPAEEQPPGDGQSRDDEGRDLNCSPTQMDPLWKVQPILGRFNAECQSLRKEDNYAVDQYPLPLTCKTRESGPSLLCTALIGLGGLLLHVDLNVDPPDKEGVVEKMAPKSSMVFLCKQELSTPAMMERLLDAGVHAAGKVGGARGQIGDEFVSSDGKLMLRRSHSGFILSTAGNGRRSMASLTSSFQKAQMSARLNRDLLNLYSTPLTATPPHSWPQAVLWYLIDLALVNSWLLYREDHGAAAGALTFMAFRLDVSKALTLSGSSDTQDSIPPQPPSEETHGTSQTPSPMLVEDSPLPDAATRYDGAGHWPEQLAEGEGGRCRFGDCQRTSRVLCLKCCVFLCISRNHNCFLNFHNQDNIGRK

Target ORF information:

RefSeq Version	XM_023964092.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes uncharacterized LOC101162913 (LOC101162913), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023964092.1

ORF Insert Sequence:

ATGCAGAGTC CATCTTTGAC CCGGACGGAC TCCATCCCTG CCTCCTCATC GGCCTCAGAG 
CAGCCCGTGG ACATGGTGGC TGAGCCGGAG CAGCAGGGAC CAGAGGGACA GGCGGGGGAC 
CACCCGGCAG TGGCTCCTGC AGACCCCCAA CCACCAGAGA CGAGTCAACA CACCCAAGGG 
GGAGCACAGA GCCCTGCAAA TGTAGCGGCC ATGCCAACAG CAGAGGCTGA AGATGATGCC 
AAGAGAAGCA CTGAGAGCAG CAGTGGAGCG AAGACAAACG TGCAGACCGT CAGGAGATAC 
GTCCCTTCCA AGAAAGCAAT GATAGATCCT CTGAAGATGG ATATGACCAA ATCGCTGGTT 
GAGCCACTCT CATCCTCTCA GCTCTCTCTG CACTGCGTCG AGTGCCACAT CATCTTCAGT 
GACCACAAGA GCAAAGACCG CCACGTGAAG CTGAAACACC CAGTAGAATA TGAAAAGCGC 
AACCTGAAAA ACGCCCTTTT TGCATGTTAC GTGTGTGACC GCCATTTTAC CAACTCCACG 
GACCTCATGG CCCACCAGAA GGCCCACACT GAGAAGAAAC CCTTCAAGTG TCCCGTGTGC 
GATCAGGCCT TCAGGAGGTC CTCCGAGCTC ACCAGTCATA AAAAAGCTCA TTTCGGCCTG 
TTCGGCTACA CCTGCACGGA GTGCGGCAAA CCCTGCAAAA CGCTGACATT GCTGAAGTAT 
CACCAGCGTA CGCACACAGG AGAGAGGCCG TACGTCTGCA AGGAGTGTGG GAAGAGGTTC 
CCCATGCCGA AAGCTTTGCA CAAACACATG ATCTCCCACT CTCCAGAGGG AGCTGAAGGA 
GAAGAAAAAG ACGACGCTGC CAAAAAACGA GCAAAGAAAG ATGAAGGTTC TCCTCCTGTC 
AAACTCCACT GCTCCATGTG CAAGGCCACT TTCAAGAACT CCAAGACTCG CCAGCGGCAC 
ATGAAAATCA AGCACAACGT CCTCCCATCA GGGCAAAGCA AACCTGTTAT CACCCCCATT 
TCGATCTCAC AGCCCACGCG GTTGCAGCTA GAGGCCGCTG GACCTCTGCA AAAAGTTGAT 
GCCAACATCG ACACAGCGCA AATACGCAAA CTTATAGAGT CGCTGGGCAC TGTGAAGAAA 
GTGAACCAAG TGGTTCTGTT GGGTCAGGTG CCGGGTTACG CTCCACCACT GGAATTGCAG 
GCGGATTCGC AGCCAGCAGG GCCTCTGGAT CCCGATCTAA GTCCACCGCA GATCGATTTT 
GGAGGACTGA AGCCGCCCGA ATCCAAGACG ACTGGACTGG ACACTTCCAA TTATCCGTGT 
GGGTTCATGG ACCAAACCAT CATTCTGGAA CCCATCACTC CTGATGGAGA AACCCCCCCC 
CTGTCAGAGC TGGCATCCTG TGTAGCAGCA GGCGAAACTG CACAGACTGT GGGAGAGGTG 
GTTCAGAGGC CCCTGACCAG TGAAATTCTC TTGGATAATA TGACCTGCCA GAACGAGGAG 
AATGACCTTG AACAAACATT CATATTAGAA CTCACCCCAG CTTTGACACC AACTGCAGAA 
CTGGAGCACT CCCAGACTGA ACCACAGAAT ATGGCTCCGT CCTCCTCTCT GGTGCTGAAC 
ACGGACCAGG AGGCTGCAGA TCAAACTGCC AAAAAGTCTG CCCTCAATGG GCAAGACGCC 
AGCCTGACAG AACCGCCACT TGTCTCCACA GCCCAGCTGG ATGGAACATC TCCTTCCTTA 
GACCCAGAGC ACAAAGAACT TCCCTCCAAC CCTTTGGATT CACCAGATAA ACCCTCACAA 
GTTGGCCCCA ATGAAGGCGA TGATGCATGT ACGAAGATGA CGGGTCAAGA CCAGTTGAGT 
GAGGGCGAAT TAAAGACACA GGAACACAAT GGAGAGCATT TGTCTGAGAA ATCTGGAATG 
GAAGGTAAGA AGAACCATTC AGAAGTGGAG TCGTCTTCTA AAGCGGCAGT CCCGTCTGAC 
ATGCAGTCAA AACATGTTCC TCAGACCTCA GAACTGGCCC TGAATGTCAT GTCTGCTCAG 
GAGCTGGTGA AAGTGCGCAA AAGAAAGCCG GCCAGGACGT TTTTCTTTCC AGGATACATT 
CAAGATCTGG CCGGGTCCAT TTACAAAGAC GACTTTCTGA TCGACGCCAA ACCAGCCAAG 
CGGCAAAGAA CAAAAAAGTC TCATCTTGTT GTGAAATTCA GTCCTCAGAG CAAAGACAAG 
AAAAATAAGA AAAAAAAGCA GGAACCGCAG CAGCCAAGAC CAGAAGATCT GACTAAAGGT 
AAAGCTCCGG CTAAGAGAAC ACCTGAGAAA AAGATGTCCT TGTCACCAAA GAAAGGAGCA 
AAGGGAAAAA AGGATGCTGC AGGGGGGGGC GGAGCATCAG GAGTTGTCAC AAAGTCCCCT 
CCAACCCCAG TTGCACAGGA GAAACAAATC AAAGAAGATG TGGGTAAAAA CAAACTAAAA 
AAAAAAAAAG TAGGGAACGA AGATGCACAA ACGAGTGGGC AGAAGGCCGC AGCTTCACCT 
GTGTTCAAAA AGAAAAAGCA ACCAAAGGTT AGACAAGAAG TTTCACCCAA AAGTGCAAAA 
ACTGAAAGAG CTAGGAAAAA AACACCAACC AAAAAAGTCA AAAAGCCAGA AGAACCGAAT 
TTAAACGCAG TACAAGACTC TCTGCTTTTA TTAAAAGGCC ATAAGCAGCC CCAGCTCAAA 
GTTTACAAGC TGGACCCTTC CAGGGCGTCG AGTCAGCCTC AGGACGCCTC CGTCTCCAAG 
AAAAAGGGAG GACGGGCAAA GAAAGCTCTG TCCCTCATGT CTTCCTTGAA GTCTTCTCAA 
CCTCCACCCG ATGCTCCGCC CAACAAGCCA AAGGCCACCA GAAAACGCAA AGCCTCGTCG 
AAAGTGGAAA CTGAAGGAGT CATAACCTCT AAGCGAGCCT TGGAGTGCAA AGACTGTGGG 
GAAAAGTTCA GTGACGTCTC ATCGCTTCAG AAGCACAAAA CGACCGCACA CACCGTAGAG 
AGTCCAAGTC TCACCTACAC CAACGGAAAC ATATTTGAGG GCGTCTCCAG GTTGGAGTTT 
TACCCGTCCC CGAAGGTGTA TGACGGCGGA GCCGCGGGAA AGAATGCAGC AACAGGGTGG 
GACACTGAGC CAGAGATGGG AGAGATGACT GTGGAGGACA GGGAGCAGAA TGTCTCTTTC 
CCAGCTCTGA TTCCATCCCC ATCTCTGCCG GTTTCTCCTC CAAATGTTGA CATAAATGCC 
TTCCAGGGGG CAAGTAGAAG AGACGAGAAC AAAGCCTCCC CTTTAGATGT TTGCTCTCGG 
CCCCGTGACA TGACTGCTGG CAAGGATCGC ACAGATTTCC TAGAGTCCGC TCAGGCGAAG 
TCCTCTGAGA GGAGGCCGCT GTTTCCACCA GAATGTGCCT CAAAGAGCCC CAACTCTGAA 
TGTGAAGCCC AGGGAACCAC CGAGGAAGAC TTCAAGGAAG ATCTACTTCT AGAGGTTGAC 
TTGGTTACTG TTGGGGAACA GAATGAGAGA GACAGAAGTT CTCTGGATGA AGACACTTCT 
ACCCAAAAAG ATTTAAACAC CAGTGATTCT GAAAGTGATA AACACCCCGA GCAGATTTGT 
GATGGAAGGA GTGAAAATGA GGTAACTTCA AAGACTGTGT TTTGCTCAAC CAATCAGGTG 
AAAGAAGAAG AGGAAGAGAT GTTGGTGCAG AGGAGAGGAG AGGACCAGAA GAGCGTTGTG 
ATGAGTAGAC GTAGGAGAAG AGGCTCAGAG CCCAGAAGAG GAGATGCCAT AAGAAAGACG 
GGATCTGTTG GAGAGTCTCA GACGGAAACT GAAAGTGAGC AGGATGAATG TCAGGTGGTT 
TATGAGATAC ACTCCGTCTT CTCGGACCAG GAGTCTGACA CGAAGACCAG CCCTGGATTC 
AGGACCATGA AAGGCCCTGC TTCTGTTGCG TTCATCCCTT CTGGTCCTTC AGCGTTTGAC 
AAATCCTCTG AAGAGCGTGT AGTGCTGGAG CTGGAGTCTG TAACCACTAG TGTTCAGGAA 
GTGATGAACG AACACAGGCT GCATGGAGAC GGGGAGACGG ACAGGGAAGC AGACCAGTCT 
CCAGGCATCG TGCTGGAGAG GTTCCTGACC TCCAGACAAA GATCTTCTGA CAAAGAGGCC 
TCCTGCAGGG CTGCAGACCA ACGAGAGGGT TTTGATGGTG TTTCTGAGAA AGAAGTACAG 
TTTGTTGGGA GCCAGGAGAT CAAAGTTGAA GAGACCCCGC CGGACCCCAC ACTGCAGCCC 
CCTGCCTGTC AAAGCAGGCA GAGGGCCGGT CTCCATCCAC AGCATAATCG AGCCATACGG 
ACCGTTCTAG TGAAAGAGGA GAGCGGCCCC CTGCTGAACG CTGCTCTGGA CGCCCAAGGC 
AGCAGACGGA TGCAGTGGGC AGTGGAGCCA ATCAGCAGCG GGGGGGCCGA TGGGCCGTTA 
ATTGAGAATG GGCAGAGCCC CAATGAGGCA CGCAGGAGTC CAGAGTTCAA CACCAAGCAG 
TGCATCTTCT ATCCAGTGAA GGAGGAGGAG CGGGAACTTC TGCTGGCATC TGCTCAGACC 
AACGCTGACA CCTTCAGCCT GGAAGGCTTC GGTGATGGAG CCCCAGCTGA GCATCAACCT 
GCAGAGTGTG ATGCATCTTT CTCTGACTCG GAGTGGCAGC CGTCAGATGT GCGGGACTTT 
CTACTCAAGA GTTGTGATGA CGACGTGAGC GGCTTCGAAC TGCCTGATCC TCAGCGTGAC 
ACAGAAGCAG AAGTGATGGC TTACTTCAAC AGGAAGCAGT CCAGCAGCTC GCATGAGCCC 
AATCACACAT CCGCGAGTTT TTCAACATCG AGTCATCTGC AACCCCAGAT GAATGGAACC 
AGGACCAGAG AGCCCATTGA TTACTTTTCC AAATATTTTG GTTGGGATAC TTGGGCAGAA 
ACTTCAACTT GCACAAACAA ACTCTGCAAA ATGTCAGATC CTGTCACGTC TGTAGACGTT 
GCACGGTTTG TTGGTATCCA CATCGCCATG GGAACTTTGA AGTTTCCCAG TCCAAGGCTT 
TACTGGGAGG ACTTGACGAA GGTGCCCCTG ATTGCTGACG CCATGCCCCT GACTCGCTTT 
GTGGAGCTGT CCCGTACGTT ACAGCTACCT TCTCCTGCTG AAGAGCAGCC ACCTGGTGAT 
GGACAGTCCA GAGATGACGA GGGGAGGGAC TTGAACTGTT CACCAACACA GATGGATCCT 
CTGTGGAAGG TCCAGCCAAT CCTGGGCCGC TTCAATGCTG AGTGCCAGTC GTTGAGAAAG 
GAGGACAACT ATGCCGTTGA CCAGTATCCA CTTCCTCTGA CCTGCAAGAC TCGCGAAAGT 
GGGCCGTCTC TGCTCTGCAC TGCACTGATT GGACTTGGTG GTTTACTCTT GCATGTGGAT 
CTCAATGTGG ATCCCCCAGA TAAAGAAGGT GTCGTAGAAA AGATGGCGCC TAAAAGTAGC 
ATGGTGTTTC TTTGCAAACA GGAGCTTTCC ACCCCTGCCA TGATGGAACG CCTTTTAGAT 
GCTGGGGTTC ACGCGGCCGG TAAGGTGGGA GGAGCTCGAG GACAAATCGG AGATGAGTTT 
GTGAGCTCCG ACGGGAAGCT GATGCTCCGC AGATCCCACA GCGGCTTCAT ACTTTCTACA 
GCGGGGAACG GTCGGAGGAG CATGGCTTCC CTCACCAGCA GCTTCCAGAA GGCCCAGATG 
TCGGCTCGCC TCAACAGAGA TTTGCTAAAC CTGTACTCCA CACCTCTCAC GGCCACGCCT 
CCACACAGCT GGCCCCAAGC GGTGCTCTGG TACCTCATCG ATTTGGCTTT GGTGAACTCC 
TGGCTTTTGT ACCGAGAAGA TCACGGGGCA GCTGCTGGAG CTTTGACTTT CATGGCTTTC 
AGGCTGGATG TTTCCAAGGC GCTGACCCTC TCCGGCAGCT CAGACACACA GGACTCCATT 
CCTCCTCAGC CTCCATCAGA GGAAACCCAC GGGACAAGCC AAACGCCCAG TCCCATGCTG 
GTGGAGGACA GCCCCCTGCC CGACGCCGCC ACCCGCTACG ACGGCGCAGG CCACTGGCCG 
GAGCAGCTGG CAGAGGGGGA AGGGGGCAGG TGTCGTTTCG GGGACTGTCA GAGGACGTCG 
CGAGTTTTGT GTCTGAAGTG TTGCGTCTTT CTCTGCATCT CACGAAATCA CAACTGCTTT 
TTGAATTTTC ATAACCAGGA CAATATAGGC AGAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101162913 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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