casz1 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	casz1
Entrez Gene ID	110532323
Full Name	castor zinc finger 1
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021616109.1	XP_021471784.1	zinc finger protein castor homolog 1 isoform X5
XM_021616111.1	XP_021471786.1	zinc finger protein castor homolog 1 isoform X7
XM_021616105.1	XP_021471780.1	zinc finger protein castor homolog 1 isoform X1
XM_021616107.1	XP_021471782.1	zinc finger protein castor homolog 1 isoform X3
XM_021616110.1	XP_021471785.1	zinc finger protein castor homolog 1 isoform X6
XM_021616106.1	XP_021471781.1	zinc finger protein castor homolog 1 isoform X2
XM_021616108.1	XP_021471783.1	zinc finger protein castor homolog 1 isoform X4

Pathways from BioSystems
Gene Ontology

The following casz1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the casz1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOf07778	XM_021616109.1 Latest version!	Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOf07779	XM_021616110.1 Latest version!	Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOf07780	XM_021616111.1 Latest version!	Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOf07774	XM_021616105.1 Latest version!	Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOf07775	XM_021616106.1 Latest version!	Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOf07776	XM_021616107.1 Latest version!	Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOf07777	XM_021616108.1 Latest version!	Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

casz1 ( XM_021616109.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021471784.1 Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OOf07778
Clone ID Related Accession (Same CDS sequence)	XM_021616109.1
Accession Version	XM_021616109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	zinc finger protein castor homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTA CATGTCTGAG AGGATTTAAC TTCGACTTCC CTGAATATGC TGAAAGGACT 
TTATGCACTG AAAAGACCTC AGGAAAATCC AACATGGCCG GCAAAAGGAA AGGTGGCCTA 
AAACTCAACG CAATCTGTGC CAAGCTGAGC CGTCAGGTGG TGTACGACGG CAGCTCCCAG 
CATGCTGAGG GAGACCACAG TGTGGCGGAG AACAGCGAGC GCGGGAGCTC CCATTATGAC 
GACAGCGGCG AGCGATTCGA GACCAACTTC CCCGAGGGCC TGAGTCTCGG CCAGAGCCTC 
GAAGAGGACC AGAAACGGCG CAAGGCCATT GAGAAGTGGG TCAACGGGGA GTATGGGGAT 
GAACCACCAC CCTCTGCCCT GGACTGTGGG GAGGAACAGC AGCAACAGCA GGGACTCAAG 
CCCAACAATG GCGAGGACGG ACCCCCCGAG GGTGTGTACA TGGTACAGCC CAAGGGCTGT 
AGTGACGAGG AGGACAATGC GCTTGATGAG CCCGAACCCA TGTTCGACTC TCGGGACGGC 
AGTTACCACG ACGACAAGGA CTCGGAAGAC GGGCCTCAAA AGGACGTCTA CATGCCGTTG 
ATAAGTGAGC CCCCGAGTCG CCAAACATCT TTCTCCTCTC CAGGAGAGGC GTCCACTCTG 
CGAGACTACG CAGCCAACAC AATGAACGAG TTCCTGGGCA TGTTCGGCTA CGACGACCAG 
CAGGTGAGGG ACGAGCTGGC CAAGAAGATC AGCTTCGAGA AGCTCAAAGC CGCTACCTCA 
GGCTCCGACC CATCCTCCCT GAGCAGCGAG GAGGCCACGC GGCGAGCCCG CTTTTCCAAG 
TATGAGGAGT ACATCCGCAA GCTGAAGGCT GGCGAGACCC TGCCCTGGCC CATCCACACC 
AACCCCAAAC AAGAGGACCT CAACTCCAAG CTTGCCCAGG AGAAGAATGC CCTGCTCCAG 
GCCCAGATGG CCGCCTCTGG GGGGTGCCAT TCCGGGGCTG AGGGACAGAT TTATCACTCC 
AGCCTGGACC ACAAGCAGGG GCAAGGGCAG GGGCACAACC TTGGGCACAA TCCCCCACCA 
ACCAACCCCA CCTCCCACCT CCAGAACATG GCCTCCCGAG CCTCTAAGTA TGACTTCTTC 
ATCCAGAAGC TGAAGATGGG TGAAAGCCTG CGGAGCCAGC AGAATGGCAA TGCTTACAAG 
AAGCCATCCA AGTACGACCT GGAGAATGTT CGATACCTGC ACCTCTTCAA GCCCGGCGAC 
GGGAACACTG ACATGGGGGG CGCCATCGCC TATAAAACGG GGAAGGTGGG CCGCCCGTCC 
AAGTATGATA TCAAGAACAT CCAGAAGCTG ATGCCGGGGA GGCCCGAGTC CTCGCTGATG 
CCCAGCGTTC TGCCTGCCAC ACCTGGGAAC CCGGGGACAC CTGTGGCTGC CACCTCGGCC 
TCCTCCGCTG GGGCCACGGG CCCCCCAGGA CTCATCATGG ATCAGACAGG CCACTTGGGC 
TTCAACACCT CCGACTACAT GAAGTCCAGC TTCTCCAAGA CCGACTCCAT CACCACGGGC 
ACTGTGTCTT CTGTCAAGAA CGGCTTACCA CCTGAGAAAC CTGTCACAGA GGACGTCAAC 
CTGTACCAGA AATATATCGC CAGGTTCTCG GGGAGCCAGC ACTGCGGACA TGTGCATTGT 
GCCTACCAAT ACAGGGAGCA TTACCACTGC ATGGACACGG AGTGCAACTA CCAGGTGGGC 
TGCAACAAGG TATACACCAG CACCTCCGAT GTCATGACTC ACGAGAACTT CCATAAGAAG 
AACGCCCAGC TGATCAACGA TGGCTTTCAG AGGTTCCGCG CCACTGAGGA TTGTGGGACG 
GTGGAGTGCC AATTCTACGG GCAGAAGACC ACCCACTTCC ACTGCAGGCG CCCGGGGTGC 
ACCTTCACCT TCAAGAACAA GTGCGACATT GAGAAGCACA AGAGCTACCA CATCAAGGAT 
GACGCATATG CCAAGGATGG CTTCAAGAAG TTCTACAAGT ATGAGGAGTG CAAATACGAG 
GGCTGTGTCT ACAGCAAGGC CACCAACCAC TTCCACTGCA TCCGCTCAAG CTGCGGCTTC 
ACCTTCACCT CCACCAGCCA GATGACCTCC CACAAGCGCA AGCACGAGCG CCGCCACATC 
CGCTCCTCGG GCGTGATGGC CCTCTCCTCT GGCGGCTCCT TCCTGATGCC CAAGGAGGAA 
CCCGGAGACG AGTCGAGCAA CGACGACCTC ATGGACTTCT CGGCCATCAG CAGCAAGAAC 
TCCAGCCTGA GCGCCTCGCC CACCACCCAG CAGTCGTCCA ACGGTCCCGG CACCCACTTG 
CTTGCCACAC CCACCACGGT GATGTCGTCC TCCGGCGGTG GCCACAGCGG CCTCAAGCCC 
ACCTCCTCGC TGCCGCACTC GGCACGCATG TCAAGCCTGC TATCCCAGGC GCTGCCCAGC 
AACATGCCTG TGGCACTGGC GCTGTCCAAC AGTGCCCTCG CCAGCGTCAA CAATCCCTTC 
TTCCCGCTCA TGCCCCGGCT GCCCATGCAG CTGCCCCATT CGGCCGCCAG CCTGATCTCG 
GCCGTGTCGT CTGGCGCCCA CTCCATGCCC ACCGACTCAC TGTCCCAGGG CGGCTGCTCC 
TCGGCGGGCA GCGATGGCGC CATGGCATCC ACGCCGACCT CCTACGCCTC AGCCTCTTCC 
ATCATGGAGA AGATCTCTGC TAGCAAGGGC CTCATATCCC CGATGATGGC CAGGCTGGCA 
GCAGCTGCCC TCAAGCCCTC TGGCAACCAC CAAGACACAG GGAATGGGCT GGCTGGTTCT 
TCAGCGAGTC TGTTCAATCT GGTTCAAGTG AAGCAGGAGC CAGTCGACCA TACCTCCGGG 
GCCTCCCAGG ACTCCATCCA AGAGCACAGC CTGGACCTGA GCAAAAAAGA CCACAGTAAT 
GAATCAAACG GACACCCTGT ACCGGGAAAT ACATCTCTTT TATCCTCGCT TATGAATAAG 
ATGAATGCCG GCTTCTTCAG TGCCCTGAAC CTGAAGACGG AGATGGAGAT CGGCCAAGGG 
GCCGACACCT CAGAGGCAGC ACAGTACCTG AGCCGCATCA TGAAGAGGCC CATGCCAGAC 
AAACCAAGCG AGCTCTGGAG GACATATCTG CGCAGGTTTG ACACGGAGGA CTTCTGCGAG 
GCCCAGTGTG ACTTCCTGCA GAAGGTCCAC TTCCATTGTC TAGTGGAGGA CTGCGGGGCG 
CTCTTCAGCA CGATGGACGG AGCCATTAAA CACGCCAACT TCCACCTACG AGCCACGCTG 
AAGGTGAAAT CAGAGCCTCA CTTCAACAAA GTCAAGGACT GCGGCGACGG AGCATCCCAC 
CAGTCCACCG CCCCTGTCTC CATGGCTAAC AACCCCGCCA TGGACGTGGC CAACATGTCA 
TCCTCGTCAG GCGGCGGCTA TGGCTCCTCC CCCTCACTGC TGGCATGGAA GCAGCTGACG 
GGCAGCATCC CACAGCTCAA TGCCTCCATG CCTAACCTGC CTGCCAATTC GCCGCTGGCC 
ACCACCTCTC TGGAGAACGC CAAGCCACAG GTCAAACCGG GCTTCCTGCA GTTCCAGGAC 
AATTCATACA GTGACCCTTG CCTGGCCACT GACTGTAAGT ACTCCAACAA GGTCCACTTC 
CACTGTCTCT TCGGGAACTG CAAGTACGTC TGCAAGACCT CGGGGAAGGC CGAATCCCAC 
TGCTTGGACC ACATCAATCC TAACAACCAC CTGGTCAACG TTCGAGACCA GTTCTCCTAC 
TTCTCCCTCC AGTGTCTATG TCCCAACCAG CACTGTGAGT TCAGAATGAG GGGCCATTAC 
CACTGTCTGC GATCCGGCTG CTACTTTGTC ACCAACATCA CCACCAAGCT TCCCTGGCAT 
GTCAAGAAGC ATGAGAAGGC AGAGAGACGC GCCGCCAATG GCTTCAAATA CTTCACCAAG 
AGAGAGGAGT GTGGCAGGCT AGGCTGCAAG TACAACCAGG TGAACAGTCA CTTCCACTGC 
ATCCGCGAGA GCTGCCAGTT CTCCTTCCTG CTCAAGCACC AGATGACCTC GCACTCCCGC 
AAACACATGA GGAGAATGCT GGGAAAGAAC TTTGACAGGG TTCCATCCCA GGTGGTGTCC 
CATGGCCAGA GGGCCGATGA CATGCAGCAC TTGTCGGGCA TGGGCTCAGG TCTCATGGGC 
AGCCACCCGG GGATGACCCA TGGGTTCTCC AGCATGATGG ATGAGACGGA TGACTACATG 
GACTACATGG GCGGGGGCAG CCCCCTGTGC CTTTCCTCTG AGTCCTCCAA TCAGGACCGC 
AGTTGCAGCA GCACCCCCGT GGGCAATGAA AGCTCCCCAG CAGGACCAGG CTGCCCGGTC 
ACTACTGCTC CCTCTACCCC CGCTGACACT AGCGCTCCCC AAAACGCACC TCCTCCACCA 
CCTCCTCCTC AGCCTGGACT TCAGTCTCAG GCTCCATCTT TCCCTCCTGC CCTCCTCCGA 
CCTCCTCTTC CCTCGCTCCC ATATCTCGTC TCTCCATCCT GTCTGTCATA CTCTCTGCTC 
AGCGCCTCTC TCGGAGCCAC TCGGAGTGTT GTCATGCCAA CCAACACACC AGCTTTCAGC 
CCCATCATTG CCACTCCGTC TCCGGTTAAA AATGACGTCC CTATAGTGCA GGATGCTGCA 
GGCAACACCA TCTCCATCCC TACGGCCACC GGCGCCAAGA AACGCTTCTG GATCATCGAG 
GACATGTCCC CCTTCGGCAA GCGGCGTAAG ACGGCTTCTG CTCGCAAAAT GCTGGACGAG 
GGCATGATGC TGGAGGGCTT CCGCCGTTAT GATCTGTACG AGGACTGCAA GGACTCCGGC 
TGCCAGTTCT CTCTGAAGGT GACCCACTAC CACTGCACCC GCGAGAACTG CGGCTACAAG 
TTCTGCGGCC GCACGCACAT GTACAAGCAT GCGCAGCACC ACGACCGCGT GGACAACCTG 
GTCCTGGACG ACTTCAAGCG TTTCAAGTCA TCGCTCAGCT GCAACTTCTC CGACTGCCAG 
TTCTCCGGCA ACAGCACCCA CTTCCACTGC CTGCGCTGCA GCTTCCGTTG CACGGACAGC 
ACCAAGGTGA CTGCCCACCG CAAACACCAT GGCAAGCAAG ATGTGATCAG CGCCGCTGGC 
TTCTGCCAGT TCAGCTCCAG CGCTGACTGC GAGGTTGTCG ACTGCAAATA CAAGCTCAAG 
TGCTCACACT TTCACTGCAC CTACCCCGAG TGCAAGCACA CTGTAGTCGG TATGTCCCAG 
ATGGACTCCC ACAAGCGCAA GCATGAGAAG CAGGAGCGCG GCGAGCTGCC GTCGGTGTCT 
CCCCGTCAGG AGGGGGCGCA CCACATGACG GGAGGAGTGT CCGCAATGCC CCACGCCTCC 
ATGGGCCTTC TTCCAGCCTC GCCCGGTGGC CTCCCAGGAC TCTCCCACCA CCACCATCAC 
CACCATAACA ACAACAACCA TGCCCCCTCC ATGCTCTACC CCCCAGCCAG CGTGGCATCT 
GACTACAACC ACCCCTATCC AACCTCCTTG GTCAGTCTGG ACAGCTCCCT CAACCTGGGC 
ACCGACACCA GCAGCTCCCT GTTCTTCCTG AAGAACGCCG CTGGCCTGGG CCTCAATGAC 
TCGTTGGACC TGAGCAAAAA GATGCAGCAT CACCATGACC CGAGCCTGAC CATGGCTGCC 
GGGCCCAACC CCATGCCACC GCTGGGCTTG CCCGCTCCTC AGGATGACAC CACGGGAACA 
TCGGGCGACC CCGAAGATGA CCAGTCACCC GAGGAAGAGG CCAATGCAGA GGAAGATGAG 
GAGGATGAGG ATGAGGCGGA GGAGGATCTG AACACTGATT CGTACGAAGA TTCGATGCCA 
CAGCCCGACG GCGACAACAA GGACAATGAC GAGAGTTTTG ACGCTTCCTT AAACCACACT 
GACACTTCCC AACTGGAAAA GCAAGAGGCC GACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021471784.1
CDS	355..6333
Translation	MKVTCLRGFNFDFPEYAERTLCTEKTSGKSNMAGKRKGGLKLNAICAKLSRQVVYDGSSQHAEGDHSVAENSERGSSHYDDSGERFETNFPEGLSLGQSLEEDQKRRKAIEKWVNGEYGDEPPPSALDCGEEQQQQQGLKPNNGEDGPPEGVYMVQPKGCSDEEDNALDEPEPMFDSRDGSYHDDKDSEDGPQKDVYMPLISEPPSRQTSFSSPGEASTLRDYAANTMNEFLGMFGYDDQQVRDELAKKISFEKLKAATSGSDPSSLSSEEATRRARFSKYEEYIRKLKAGETLPWPIHTNPKQEDLNSKLAQEKNALLQAQMAASGGCHSGAEGQIYHSSLDHKQGQGQGHNLGHNPPPTNPTSHLQNMASRASKYDFFIQKLKMGESLRSQQNGNAYKKPSKYDLENVRYLHLFKPGDGNTDMGGAIAYKTGKVGRPSKYDIKNIQKLMPGRPESSLMPSVLPATPGNPGTPVAATSASSAGATGPPGLIMDQTGHLGFNTSDYMKSSFSKTDSITTGTVSSVKNGLPPEKPVTEDVNLYQKYIARFSGSQHCGHVHCAYQYREHYHCMDTECNYQVGCNKVYTSTSDVMTHENFHKKNAQLINDGFQRFRATEDCGTVECQFYGQKTTHFHCRRPGCTFTFKNKCDIEKHKSYHIKDDAYAKDGFKKFYKYEECKYEGCVYSKATNHFHCIRSSCGFTFTSTSQMTSHKRKHERRHIRSSGVMALSSGGSFLMPKEEPGDESSNDDLMDFSAISSKNSSLSASPTTQQSSNGPGTHLLATPTTVMSSSGGGHSGLKPTSSLPHSARMSSLLSQALPSNMPVALALSNSALASVNNPFFPLMPRLPMQLPHSAASLISAVSSGAHSMPTDSLSQGGCSSAGSDGAMASTPTSYASASSIMEKISASKGLISPMMARLAAAALKPSGNHQDTGNGLAGSSASLFNLVQVKQEPVDHTSGASQDSIQEHSLDLSKKDHSNESNGHPVPGNTSLLSSLMNKMNAGFFSALNLKTEMEIGQGADTSEAAQYLSRIMKRPMPDKPSELWRTYLRRFDTEDFCEAQCDFLQKVHFHCLVEDCGALFSTMDGAIKHANFHLRATLKVKSEPHFNKVKDCGDGASHQSTAPVSMANNPAMDVANMSSSSGGGYGSSPSLLAWKQLTGSIPQLNASMPNLPANSPLATTSLENAKPQVKPGFLQFQDNSYSDPCLATDCKYSNKVHFHCLFGNCKYVCKTSGKAESHCLDHINPNNHLVNVRDQFSYFSLQCLCPNQHCEFRMRGHYHCLRSGCYFVTNITTKLPWHVKKHEKAERRAANGFKYFTKREECGRLGCKYNQVNSHFHCIRESCQFSFLLKHQMTSHSRKHMRRMLGKNFDRVPSQVVSHGQRADDMQHLSGMGSGLMGSHPGMTHGFSSMMDETDDYMDYMGGGSPLCLSSESSNQDRSCSSTPVGNESSPAGPGCPVTTAPSTPADTSAPQNAPPPPPPPQPGLQSQAPSFPPALLRPPLPSLPYLVSPSCLSYSLLSASLGATRSVVMPTNTPAFSPIIATPSPVKNDVPIVQDAAGNTISIPTATGAKKRFWIIEDMSPFGKRRKTASARKMLDEGMMLEGFRRYDLYEDCKDSGCQFSLKVTHYHCTRENCGYKFCGRTHMYKHAQHHDRVDNLVLDDFKRFKSSLSCNFSDCQFSGNSTHFHCLRCSFRCTDSTKVTAHRKHHGKQDVISAAGFCQFSSSADCEVVDCKYKLKCSHFHCTYPECKHTVVGMSQMDSHKRKHEKQERGELPSVSPRQEGAHHMTGGVSAMPHASMGLLPASPGGLPGLSHHHHHHHNNNNHAPSMLYPPASVASDYNHPYPTSLVSLDSSLNLGTDTSSSLFFLKNAAGLGLNDSLDLSKKMQHHHDPSLTMAAGPNPMPPLGLPAPQDDTTGTSGDPEDDQSPEEEANAEEDEEDEDEAEEDLNTDSYEDSMPQPDGDNKDNDESFDASLNHTDTSQLEKQEADP

Target ORF information:

RefSeq Version	XM_021616109.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021616109.1

ORF Insert Sequence:

ATGAAAGTTA CATGTCTGAG AGGATTTAAC TTCGACTTCC CTGAATATGC TGAAAGGACT 
TTATGCACTG AAAAGACCTC AGGAAAATCC AACATGGCCG GCAAAAGGAA AGGTGGCCTA 
AAACTCAACG CAATCTGTGC CAAGCTGAGC CGTCAGGTGG TGTACGACGG CAGCTCCCAG 
CATGCTGAGG GAGACCACAG TGTGGCGGAG AACAGCGAGC GCGGGAGCTC CCATTATGAC 
GACAGCGGCG AGCGATTCGA GACCAACTTC CCCGAGGGCC TGAGTCTCGG CCAGAGCCTC 
GAAGAGGACC AGAAACGGCG CAAGGCCATT GAGAAGTGGG TCAACGGGGA GTATGGGGAT 
GAACCACCAC CCTCTGCCCT GGACTGTGGG GAGGAACAGC AGCAACAGCA GGGACTCAAG 
CCCAACAATG GCGAGGACGG ACCCCCCGAG GGTGTGTACA TGGTACAGCC CAAGGGCTGT 
AGTGACGAGG AGGACAATGC GCTTGATGAG CCCGAACCCA TGTTCGACTC TCGGGACGGC 
AGTTACCACG ACGACAAGGA CTCGGAAGAC GGGCCTCAAA AGGACGTCTA CATGCCGTTG 
ATAAGTGAGC CCCCGAGTCG CCAAACATCT TTCTCCTCTC CAGGAGAGGC GTCCACTCTG 
CGAGACTACG CAGCCAACAC AATGAACGAG TTCCTGGGCA TGTTCGGCTA CGACGACCAG 
CAGGTGAGGG ACGAGCTGGC CAAGAAGATC AGCTTCGAGA AGCTCAAAGC CGCTACCTCA 
GGCTCCGACC CATCCTCCCT GAGCAGCGAG GAGGCCACGC GGCGAGCCCG CTTTTCCAAG 
TATGAGGAGT ACATCCGCAA GCTGAAGGCT GGCGAGACCC TGCCCTGGCC CATCCACACC 
AACCCCAAAC AAGAGGACCT CAACTCCAAG CTTGCCCAGG AGAAGAATGC CCTGCTCCAG 
GCCCAGATGG CCGCCTCTGG GGGGTGCCAT TCCGGGGCTG AGGGACAGAT TTATCACTCC 
AGCCTGGACC ACAAGCAGGG GCAAGGGCAG GGGCACAACC TTGGGCACAA TCCCCCACCA 
ACCAACCCCA CCTCCCACCT CCAGAACATG GCCTCCCGAG CCTCTAAGTA TGACTTCTTC 
ATCCAGAAGC TGAAGATGGG TGAAAGCCTG CGGAGCCAGC AGAATGGCAA TGCTTACAAG 
AAGCCATCCA AGTACGACCT GGAGAATGTT CGATACCTGC ACCTCTTCAA GCCCGGCGAC 
GGGAACACTG ACATGGGGGG CGCCATCGCC TATAAAACGG GGAAGGTGGG CCGCCCGTCC 
AAGTATGATA TCAAGAACAT CCAGAAGCTG ATGCCGGGGA GGCCCGAGTC CTCGCTGATG 
CCCAGCGTTC TGCCTGCCAC ACCTGGGAAC CCGGGGACAC CTGTGGCTGC CACCTCGGCC 
TCCTCCGCTG GGGCCACGGG CCCCCCAGGA CTCATCATGG ATCAGACAGG CCACTTGGGC 
TTCAACACCT CCGACTACAT GAAGTCCAGC TTCTCCAAGA CCGACTCCAT CACCACGGGC 
ACTGTGTCTT CTGTCAAGAA CGGCTTACCA CCTGAGAAAC CTGTCACAGA GGACGTCAAC 
CTGTACCAGA AATATATCGC CAGGTTCTCG GGGAGCCAGC ACTGCGGACA TGTGCATTGT 
GCCTACCAAT ACAGGGAGCA TTACCACTGC ATGGACACGG AGTGCAACTA CCAGGTGGGC 
TGCAACAAGG TATACACCAG CACCTCCGAT GTCATGACTC ACGAGAACTT CCATAAGAAG 
AACGCCCAGC TGATCAACGA TGGCTTTCAG AGGTTCCGCG CCACTGAGGA TTGTGGGACG 
GTGGAGTGCC AATTCTACGG GCAGAAGACC ACCCACTTCC ACTGCAGGCG CCCGGGGTGC 
ACCTTCACCT TCAAGAACAA GTGCGACATT GAGAAGCACA AGAGCTACCA CATCAAGGAT 
GACGCATATG CCAAGGATGG CTTCAAGAAG TTCTACAAGT ATGAGGAGTG CAAATACGAG 
GGCTGTGTCT ACAGCAAGGC CACCAACCAC TTCCACTGCA TCCGCTCAAG CTGCGGCTTC 
ACCTTCACCT CCACCAGCCA GATGACCTCC CACAAGCGCA AGCACGAGCG CCGCCACATC 
CGCTCCTCGG GCGTGATGGC CCTCTCCTCT GGCGGCTCCT TCCTGATGCC CAAGGAGGAA 
CCCGGAGACG AGTCGAGCAA CGACGACCTC ATGGACTTCT CGGCCATCAG CAGCAAGAAC 
TCCAGCCTGA GCGCCTCGCC CACCACCCAG CAGTCGTCCA ACGGTCCCGG CACCCACTTG 
CTTGCCACAC CCACCACGGT GATGTCGTCC TCCGGCGGTG GCCACAGCGG CCTCAAGCCC 
ACCTCCTCGC TGCCGCACTC GGCACGCATG TCAAGCCTGC TATCCCAGGC GCTGCCCAGC 
AACATGCCTG TGGCACTGGC GCTGTCCAAC AGTGCCCTCG CCAGCGTCAA CAATCCCTTC 
TTCCCGCTCA TGCCCCGGCT GCCCATGCAG CTGCCCCATT CGGCCGCCAG CCTGATCTCG 
GCCGTGTCGT CTGGCGCCCA CTCCATGCCC ACCGACTCAC TGTCCCAGGG CGGCTGCTCC 
TCGGCGGGCA GCGATGGCGC CATGGCATCC ACGCCGACCT CCTACGCCTC AGCCTCTTCC 
ATCATGGAGA AGATCTCTGC TAGCAAGGGC CTCATATCCC CGATGATGGC CAGGCTGGCA 
GCAGCTGCCC TCAAGCCCTC TGGCAACCAC CAAGACACAG GGAATGGGCT GGCTGGTTCT 
TCAGCGAGTC TGTTCAATCT GGTTCAAGTG AAGCAGGAGC CAGTCGACCA TACCTCCGGG 
GCCTCCCAGG ACTCCATCCA AGAGCACAGC CTGGACCTGA GCAAAAAAGA CCACAGTAAT 
GAATCAAACG GACACCCTGT ACCGGGAAAT ACATCTCTTT TATCCTCGCT TATGAATAAG 
ATGAATGCCG GCTTCTTCAG TGCCCTGAAC CTGAAGACGG AGATGGAGAT CGGCCAAGGG 
GCCGACACCT CAGAGGCAGC ACAGTACCTG AGCCGCATCA TGAAGAGGCC CATGCCAGAC 
AAACCAAGCG AGCTCTGGAG GACATATCTG CGCAGGTTTG ACACGGAGGA CTTCTGCGAG 
GCCCAGTGTG ACTTCCTGCA GAAGGTCCAC TTCCATTGTC TAGTGGAGGA CTGCGGGGCG 
CTCTTCAGCA CGATGGACGG AGCCATTAAA CACGCCAACT TCCACCTACG AGCCACGCTG 
AAGGTGAAAT CAGAGCCTCA CTTCAACAAA GTCAAGGACT GCGGCGACGG AGCATCCCAC 
CAGTCCACCG CCCCTGTCTC CATGGCTAAC AACCCCGCCA TGGACGTGGC CAACATGTCA 
TCCTCGTCAG GCGGCGGCTA TGGCTCCTCC CCCTCACTGC TGGCATGGAA GCAGCTGACG 
GGCAGCATCC CACAGCTCAA TGCCTCCATG CCTAACCTGC CTGCCAATTC GCCGCTGGCC 
ACCACCTCTC TGGAGAACGC CAAGCCACAG GTCAAACCGG GCTTCCTGCA GTTCCAGGAC 
AATTCATACA GTGACCCTTG CCTGGCCACT GACTGTAAGT ACTCCAACAA GGTCCACTTC 
CACTGTCTCT TCGGGAACTG CAAGTACGTC TGCAAGACCT CGGGGAAGGC CGAATCCCAC 
TGCTTGGACC ACATCAATCC TAACAACCAC CTGGTCAACG TTCGAGACCA GTTCTCCTAC 
TTCTCCCTCC AGTGTCTATG TCCCAACCAG CACTGTGAGT TCAGAATGAG GGGCCATTAC 
CACTGTCTGC GATCCGGCTG CTACTTTGTC ACCAACATCA CCACCAAGCT TCCCTGGCAT 
GTCAAGAAGC ATGAGAAGGC AGAGAGACGC GCCGCCAATG GCTTCAAATA CTTCACCAAG 
AGAGAGGAGT GTGGCAGGCT AGGCTGCAAG TACAACCAGG TGAACAGTCA CTTCCACTGC 
ATCCGCGAGA GCTGCCAGTT CTCCTTCCTG CTCAAGCACC AGATGACCTC GCACTCCCGC 
AAACACATGA GGAGAATGCT GGGAAAGAAC TTTGACAGGG TTCCATCCCA GGTGGTGTCC 
CATGGCCAGA GGGCCGATGA CATGCAGCAC TTGTCGGGCA TGGGCTCAGG TCTCATGGGC 
AGCCACCCGG GGATGACCCA TGGGTTCTCC AGCATGATGG ATGAGACGGA TGACTACATG 
GACTACATGG GCGGGGGCAG CCCCCTGTGC CTTTCCTCTG AGTCCTCCAA TCAGGACCGC 
AGTTGCAGCA GCACCCCCGT GGGCAATGAA AGCTCCCCAG CAGGACCAGG CTGCCCGGTC 
ACTACTGCTC CCTCTACCCC CGCTGACACT AGCGCTCCCC AAAACGCACC TCCTCCACCA 
CCTCCTCCTC AGCCTGGACT TCAGTCTCAG GCTCCATCTT TCCCTCCTGC CCTCCTCCGA 
CCTCCTCTTC CCTCGCTCCC ATATCTCGTC TCTCCATCCT GTCTGTCATA CTCTCTGCTC 
AGCGCCTCTC TCGGAGCCAC TCGGAGTGTT GTCATGCCAA CCAACACACC AGCTTTCAGC 
CCCATCATTG CCACTCCGTC TCCGGTTAAA AATGACGTCC CTATAGTGCA GGATGCTGCA 
GGCAACACCA TCTCCATCCC TACGGCCACC GGCGCCAAGA AACGCTTCTG GATCATCGAG 
GACATGTCCC CCTTCGGCAA GCGGCGTAAG ACGGCTTCTG CTCGCAAAAT GCTGGACGAG 
GGCATGATGC TGGAGGGCTT CCGCCGTTAT GATCTGTACG AGGACTGCAA GGACTCCGGC 
TGCCAGTTCT CTCTGAAGGT GACCCACTAC CACTGCACCC GCGAGAACTG CGGCTACAAG 
TTCTGCGGCC GCACGCACAT GTACAAGCAT GCGCAGCACC ACGACCGCGT GGACAACCTG 
GTCCTGGACG ACTTCAAGCG TTTCAAGTCA TCGCTCAGCT GCAACTTCTC CGACTGCCAG 
TTCTCCGGCA ACAGCACCCA CTTCCACTGC CTGCGCTGCA GCTTCCGTTG CACGGACAGC 
ACCAAGGTGA CTGCCCACCG CAAACACCAT GGCAAGCAAG ATGTGATCAG CGCCGCTGGC 
TTCTGCCAGT TCAGCTCCAG CGCTGACTGC GAGGTTGTCG ACTGCAAATA CAAGCTCAAG 
TGCTCACACT TTCACTGCAC CTACCCCGAG TGCAAGCACA CTGTAGTCGG TATGTCCCAG 
ATGGACTCCC ACAAGCGCAA GCATGAGAAG CAGGAGCGCG GCGAGCTGCC GTCGGTGTCT 
CCCCGTCAGG AGGGGGCGCA CCACATGACG GGAGGAGTGT CCGCAATGCC CCACGCCTCC 
ATGGGCCTTC TTCCAGCCTC GCCCGGTGGC CTCCCAGGAC TCTCCCACCA CCACCATCAC 
CACCATAACA ACAACAACCA TGCCCCCTCC ATGCTCTACC CCCCAGCCAG CGTGGCATCT 
GACTACAACC ACCCCTATCC AACCTCCTTG GTCAGTCTGG ACAGCTCCCT CAACCTGGGC 
ACCGACACCA GCAGCTCCCT GTTCTTCCTG AAGAACGCCG CTGGCCTGGG CCTCAATGAC 
TCGTTGGACC TGAGCAAAAA GATGCAGCAT CACCATGACC CGAGCCTGAC CATGGCTGCC 
GGGCCCAACC CCATGCCACC GCTGGGCTTG CCCGCTCCTC AGGATGACAC CACGGGAACA 
TCGGGCGACC CCGAAGATGA CCAGTCACCC GAGGAAGAGG CCAATGCAGA GGAAGATGAG 
GAGGATGAGG ATGAGGCGGA GGAGGATCTG AACACTGATT CGTACGAAGA TTCGATGCCA 
CAGCCCGACG GCGACAACAA GGACAATGAC GAGAGTTTTG ACGCTTCCTT AAACCACACT 
GACACTTCCC AACTGGAAAA GCAAGAGGCC GACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

casz1 ( XM_021616110.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021471785.1 Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X6, mRNA.

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CloneID	OOf07779
Clone ID Related Accession (Same CDS sequence)	XM_021616110.1
Accession Version	XM_021616110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	zinc finger protein castor homolog 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTA CATGTCTGAG AGGATTTAAC TTCGACTTCC CTGAATATGC TGAAAGGACT 
TTATGCACTG AAAAGACCTC AGGAAAATCC AACATGGCCG GCAAAAGGAA AGGTGGCCTA 
AAACTCAACG CAATCTGTGC CAAGCTGAGC CGTCAGGTGG TGTACGACGG CAGCTCCCAG 
CATGCTGAGG GAGACCACAG TGTGGCGGAG AACAGCGAGC GCGGGAGCTC CCATTATGAC 
GACAGCGGCG AGCGATTCGA GACCAACTTC CCCGAGGGCC TGAGTCTCGG CCAGAGCCTC 
GAAGAGGACC AGAAACGGCG CAAGGCCATT GAGAAGTGGG TCAACGGGGA GTATGGGGAT 
GAACCACCAC CCTCTGCCCT GGACTGTGGG GAGGAACAGC AGCAACAGCA GGGACTCAAG 
CCCAACAATG GCGAGGACGG ACCCCCCGAG GGTGTGTACA TGGTACAGCC CAAGGGCTGT 
AGTGACGAGG AGGACAATGC GCTTGATGAG CCCGAACCCA TGTTCGACTC TCGGGACGGC 
AGTTACCACG ACGACAAGGA CTCGGAAGAC GGGCCTCAAA AGGACGTCTA CATGCCGTTG 
ATAAGTGAGC CCCCGAGTCG CCAAACATCT TTCTCCTCTC CAGGAGAGGC GTCCACTCTG 
CGAGACTACG CAGCCAACAC AATGAACGAG TTCCTGGGCA TGTTCGGCTA CGACGACCAG 
CAGGTGAGGG ACGAGCTGGC CAAGAAGATC AGCTTCGAGA AGCTCAAAGC CGCTACCTCA 
GGCTCCGACC CATCCTCCCT GAGCAGCGAG GAGGCCACGC GGCGAGCCCG CTTTTCCAAG 
TATGAGGAGT ACATCCGCAA GCTGAAGGCT GGCGAGACCC TGCCCTGGCC CATCCACACC 
AACCCCAAAC AAGAGGACCT CAACTCCAAG CTTGCCCAGG AGAAGAATGC CCTGCTCCAG 
GCCCAGATGG CCGCCTCTGG GGGGTGCCAT TCCGGGGCTG AGGGACAGAT TTATCACTCC 
AGCCTGGACC ACAAGCAGGG GCAAGGGCAG GGGCACAACC TTGGGCACAA TCCCCCACCA 
ACCAACCCCA CCTCCCACCT CCAGAACATG GCCTCCCGAG CCTCTAAGTA TGACTTCTTC 
ATCCAGAAGC TGAAGATGGG TGAAAGCCTG CGGAGCCAGC AGAATGGCAA TGCTTACAAG 
AAGCCATCCA AGTACGACCT GGAGAATGTT CGATACCTGC ACCTCTTCAA GCCCGGCGAC 
GGGAACACTG ACATGGGGGG CGCCATCGCC TATAAAACGG GGAAGGTGGG CCGCCCGTCC 
AAGTATGATA TCAAGAACAT CCAGAAGCTG ATGCCGGGGA GGCCCGAGTC CTCGCTGATG 
CCCAGCGTTC TGCCTGCCAC ACCTGGGAAC CCGGGGACAC CTGTGGCTGC CACCTCGGCC 
TCCTCCGCTG GGGCCACGGG CCCCCCAGGA CTCATCATGG ATCAGACAGG CCACTTGGGC 
TTCAACACCT CCGACTACAT GAAGTCCAGC TTCTCCAAGA CCGACTCCAT CACCACGGGC 
ACTGTGTCTT CTGTCAAGAA CGGCTTACCA CCTGAGAAAC CTGTCACAGA GGACGTCAAC 
CTGTACCAGA AATATATCGC CAGAAGCCGT CAAACCTTCC CGAAGCCCCT ACCACCACCA 
CCACCACCAC CATACCCTGA GCCCCCCACC ACACCACCAA CTTTTAAAAG GTTCTCGGGG 
AGCCAGCACT GCGGACATGT GCATTGTGCC TACCAATACA GGGAGCATTA CCACTGCATG 
GACACGGAGT GCAACTACCA GAGGTTCACC AGCAAGCAAG ACGTGATCAG GCACTACAAT 
ATGCACAAGA AGAGGGACAA CTCCCTACAG CATGGCTTCA TGCGCTTCAG CCCACTGGAC 
GACTGTAGCG TCTACTACCA TGGCTGCCAC CTCAACGGGA AGAGCACCCA CTACCACTGC 
ATGCAGGTGG GCTGCAACAA GGTATACACC AGCACCTCCG ATGTCATGAC TCACGAGAAC 
TTCCATAAGA AGAACGCCCA GCTGATCAAC GATGGCTTTC AGAGGTTCCG CGCCACTGAG 
GATTGTGGGA CGGTGGAGTG CCAATTCTAC GGGCAGAAGA CCACCCACTT CCACTGCAGG 
CGCCCGGGGT GCACCTTCAC CTTCAAGAAC AAGTGCGACA TTGAGAAGCA CAAGAGCTAC 
CACATCAAGG ATGACGCATA TGCCAAGGAT GGCTTCAAGA AGTTCTACAA GTATGAGGAG 
TGCAAATACG AGGGCTGTGT CTACAGCAAG GCCACCAACC ACTTCCACTG CATCCGCTCA 
AGCTGCGGCT TCACCTTCAC CTCCACCAGC CAGATGACCT CCCACAAGCG CAAGCACGAG 
CGCCGCCACA TCCGCTCCTC GGGCGTGATG GCCCTCTCCT CTGGCGGCTC CTTCCTGATG 
CCCAAGGAGG AACCCGGAGA CGAGTCGAGC AACGACGACC TCATGGACTT CTCGGCCATC 
AGCAGCAAGA ACTCCAGCCT GAGCGCCTCG CCCACCACCC AGCAGTCGTC CAACGGTCCC 
GGCACCCACT TGCTTGCCAC ACCCACCACG GTGATGTCGT CCTCCGGCGG TGGCCACAGC 
GGCCTCAAGC CCACCTCCTC GCTGCCGCAC TCGGCACGCA TGTCAAGCCT GCTATCCCAG 
GCGCTGCCCA GCAACATGCC TGTGGCACTG GCGCTGTCCA ACAGTGCCCT CGCCAGCGTC 
AACAATCCCT TCTTCCCGCT CATGCCCCGG CTGCCCATGC AGCTGCCCCA TTCGGCCGCC 
AGCCTGATCT CGGCCGTGTC GTCTGGCGCC CACTCCATGC CCACCGACTC ACTGTCCCAG 
GGCGGCTGCT CCTCGGCGGG CAGCGATGGC GCCATGGCAT CCACGCCGAC CTCCTACGCC 
TCAGCCTCTT CCATCATGGA GAAGATCTCT GCTAGCAAGG GCCTCATATC CCCGATGATG 
GCCAGGCTGG CAGCAGCTGC CCTCAAGCCC TCTGGCAACC ACCAAGACAC AGGGAATGGG 
CTGGCTGGTT CTTCAGCGAG TCTGTTCAAT CTGGTTCAAG TGAAGCAGGA GCCAGTCGAC 
CATACCTCCG GGGCCTCCCA GGACTCCATC CAAGAGCACA GCCTGGACCT GAGCAAAAAA 
GACCACAGTA ATGAATCAAA CGGACACCCT GTACCGGGAA ATACATCTCT TTTATCCTCG 
CTTATGAATA AGATGAATGC CGGCTTCTTC AGTGCCCTGA ACCTGAAGAC GGAGATGGAG 
ATCGGCCAAG GGGCCGACAC CTCAGAGGCA GCACAGTACC TGAGCCGCAT CATGAAGAGG 
CCCATGCCAG ACAAACCAAG CGAGCTCTGG AGGACATATC TGCGCAGGTT TGACACGGAG 
GACTTCTGCG AGGCCCAGTG TGACTTCCTG CAGAAGGTCC ACTTCCATTG TCTAGTGGAG 
GACTGCGGGG CGCTCTTCAG CACGATGGAC GGAGCCATTA AACACGCCAA CTTCCACCTA 
CGAGCCACGC TGAAGGTGAA ATCAGAGCCT CACTTCAACA AAGTCAAGGA CTGCGGCGAC 
GGAGCATCCC ACCAGTCCAC CGCCCCTGTC TCCATGGCTA ACAACCCCGC CATGGACGTG 
GCCAACATGT CATCCTCGTC AGGCGGCGGC TATGGCTCCT CCCCCTCACT GCTGGCATGG 
AAGCAGCTGA CGGGCAGCAT CCCACAGCTC AATGCCTCCA TGCCTAACCT GCCTGCCAAT 
TCGCCGCTGG CCACCACCTC TCTGGAGAAC GCCAAGCCAC AGGTCAAACC GGGCTTCCTG 
CAGTTCCAGG ACAATTCATA CAGTGACCCT TGCCTGGCCA CTGACTGTAA GTACTCCAAC 
AAGGTCCACT TCCACTGTCT CTTCGGGAAC TGCAAGTACG TCTGCAAGAC CTCGGGGAAG 
GCCGAATCCC ACTGCTTGGA CCACATCAAT CCTAACAACC ACCTGGTCAA CGTTCGAGAC 
CAGTTCTCCT ACTTCTCCCT CCAGTGTCTA TGTCCCAACC AGCACTGTGA GTTCAGAATG 
AGGGGCCATT ACCACTGTCT GCGATCCGGC TGCTACTTTG TCACCAACAT CACCACCAAG 
CTTCCCTGGC ATGTCAAGAA GCATGAGAAG GCAGAGAGAC GCGCCGCCAA TGGCTTCAAA 
TACTTCACCA AGAGAGAGGA GTGTGGCAGG CTAGGCTGCA AGTACAACCA GGTGAACAGT 
CACTTCCACT GCATCCGCGA GAGCTGCCAG TTCTCCTTCC TGCTCAAGCA CCAGATGACC 
TCGCACTCCC GCAAACACAT GAGGAGAATG CTGGGAAAGA ACTTTGACAG GGTTCCATCC 
CAGGTGGTGT CCCATGGCCA GAGGGCCGAT GACATGCAGC ACTTGTCGGG CATGGGCTCA 
GGTCTCATGG GCAGCCACCC GGGGATGACC CATGGGTTCT CCAGCATGAT GGATGAGACG 
GATGACTACA TGGACTACAT GGGCGGGGGC AGCCCCCTGT GCCTTTCCTC TGAGTCCTCC 
AATCAGGACC GCAGTTGCAG CAGCACCCCC GTGGGCAATG AAAGCTCCCC AGCAGGCAAC 
ACCATCTCCA TCCCTACGGC CACCGGCGCC AAGAAACGCT TCTGGATCAT CGAGGACATG 
TCCCCCTTCG GCAAGCGGCG TAAGACGGCT TCTGCTCGCA AAATGCTGGA CGAGGGCATG 
ATGCTGGAGG GCTTCCGCCG TTATGATCTG TACGAGGACT GCAAGGACTC CGGCTGCCAG 
TTCTCTCTGA AGGTGACCCA CTACCACTGC ACCCGCGAGA ACTGCGGCTA CAAGTTCTGC 
GGCCGCACGC ACATGTACAA GCATGCGCAG CACCACGACC GCGTGGACAA CCTGGTCCTG 
GACGACTTCA AGCGTTTCAA GTCATCGCTC AGCTGCAACT TCTCCGACTG CCAGTTCTCC 
GGCAACAGCA CCCACTTCCA CTGCCTGCGC TGCAGCTTCC GTTGCACGGA CAGCACCAAG 
GTGACTGCCC ACCGCAAACA CCATGGCAAG CAAGATGTGA TCAGCGCCGC TGGCTTCTGC 
CAGTTCAGCT CCAGCGCTGA CTGCGAGGTT GTCGACTGCA AATACAAGCT CAAGTGCTCA 
CACTTTCACT GCACCTACCC CGAGTGCAAG CACACTGTAG TCGGTATGTC CCAGATGGAC 
TCCCACAAGC GCAAGCATGA GAAGCAGGAG CGCGGCGAGC TGCCGTCGGT GTCTCCCCGT 
CAGGAGGGGG CGCACCACAT GACGGGAGGA GTGTCCGCAA TGCCCCACGC CTCCATGGGC 
CTTCTTCCAG CCTCGCCCGG TGGCCTCCCA GGACTCTCCC ACCACCACCA TCACCACCAT 
AACAACAACA ACCATGCCCC CTCCATGCTC TACCCCCCAG CCAGCGTGGC ATCTGACTAC 
AACCACCCCT ATCCAACCTC CTTGGTCAGT CTGGACAGCT CCCTCAACCT GGGCACCGAC 
ACCAGCAGCT CCCTGTTCTT CCTGAAGAAC GCCGCTGGCC TGGGCCTCAA TGACTCGTTG 
GACCTGAGCA AAAAGATGCA GCATCACCAT GACCCGAGCC TGACCATGGC TGCCGGGCCC 
AACCCCATGC CACCGCTGGG CTTGCCCGCT CCTCAGGATG ACACCACGGG AACATCGGGC 
GACCCCGAAG ATGACCAGTC ACCCGAGGAA GAGGCCAATG CAGAGGAAGA TGAGGAGGAT 
GAGGATGAGG CGGAGGAGGA TCTGAACACT GATTCGTACG AAGATTCGAT GCCACAGCCC 
GACGGCGACA ACAAGGACAA TGACGAGAGT TTTGACGCTT CCTTAAACCA CACTGACACT 
TCCCAACTGG AAAAGCAAGA GGCCGACCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021471785.1
CDS	356..6268
Translation	MKVTCLRGFNFDFPEYAERTLCTEKTSGKSNMAGKRKGGLKLNAICAKLSRQVVYDGSSQHAEGDHSVAENSERGSSHYDDSGERFETNFPEGLSLGQSLEEDQKRRKAIEKWVNGEYGDEPPPSALDCGEEQQQQQGLKPNNGEDGPPEGVYMVQPKGCSDEEDNALDEPEPMFDSRDGSYHDDKDSEDGPQKDVYMPLISEPPSRQTSFSSPGEASTLRDYAANTMNEFLGMFGYDDQQVRDELAKKISFEKLKAATSGSDPSSLSSEEATRRARFSKYEEYIRKLKAGETLPWPIHTNPKQEDLNSKLAQEKNALLQAQMAASGGCHSGAEGQIYHSSLDHKQGQGQGHNLGHNPPPTNPTSHLQNMASRASKYDFFIQKLKMGESLRSQQNGNAYKKPSKYDLENVRYLHLFKPGDGNTDMGGAIAYKTGKVGRPSKYDIKNIQKLMPGRPESSLMPSVLPATPGNPGTPVAATSASSAGATGPPGLIMDQTGHLGFNTSDYMKSSFSKTDSITTGTVSSVKNGLPPEKPVTEDVNLYQKYIARSRQTFPKPLPPPPPPPYPEPPTTPPTFKRFSGSQHCGHVHCAYQYREHYHCMDTECNYQRFTSKQDVIRHYNMHKKRDNSLQHGFMRFSPLDDCSVYYHGCHLNGKSTHYHCMQVGCNKVYTSTSDVMTHENFHKKNAQLINDGFQRFRATEDCGTVECQFYGQKTTHFHCRRPGCTFTFKNKCDIEKHKSYHIKDDAYAKDGFKKFYKYEECKYEGCVYSKATNHFHCIRSSCGFTFTSTSQMTSHKRKHERRHIRSSGVMALSSGGSFLMPKEEPGDESSNDDLMDFSAISSKNSSLSASPTTQQSSNGPGTHLLATPTTVMSSSGGGHSGLKPTSSLPHSARMSSLLSQALPSNMPVALALSNSALASVNNPFFPLMPRLPMQLPHSAASLISAVSSGAHSMPTDSLSQGGCSSAGSDGAMASTPTSYASASSIMEKISASKGLISPMMARLAAAALKPSGNHQDTGNGLAGSSASLFNLVQVKQEPVDHTSGASQDSIQEHSLDLSKKDHSNESNGHPVPGNTSLLSSLMNKMNAGFFSALNLKTEMEIGQGADTSEAAQYLSRIMKRPMPDKPSELWRTYLRRFDTEDFCEAQCDFLQKVHFHCLVEDCGALFSTMDGAIKHANFHLRATLKVKSEPHFNKVKDCGDGASHQSTAPVSMANNPAMDVANMSSSSGGGYGSSPSLLAWKQLTGSIPQLNASMPNLPANSPLATTSLENAKPQVKPGFLQFQDNSYSDPCLATDCKYSNKVHFHCLFGNCKYVCKTSGKAESHCLDHINPNNHLVNVRDQFSYFSLQCLCPNQHCEFRMRGHYHCLRSGCYFVTNITTKLPWHVKKHEKAERRAANGFKYFTKREECGRLGCKYNQVNSHFHCIRESCQFSFLLKHQMTSHSRKHMRRMLGKNFDRVPSQVVSHGQRADDMQHLSGMGSGLMGSHPGMTHGFSSMMDETDDYMDYMGGGSPLCLSSESSNQDRSCSSTPVGNESSPAGNTISIPTATGAKKRFWIIEDMSPFGKRRKTASARKMLDEGMMLEGFRRYDLYEDCKDSGCQFSLKVTHYHCTRENCGYKFCGRTHMYKHAQHHDRVDNLVLDDFKRFKSSLSCNFSDCQFSGNSTHFHCLRCSFRCTDSTKVTAHRKHHGKQDVISAAGFCQFSSSADCEVVDCKYKLKCSHFHCTYPECKHTVVGMSQMDSHKRKHEKQERGELPSVSPRQEGAHHMTGGVSAMPHASMGLLPASPGGLPGLSHHHHHHHNNNNHAPSMLYPPASVASDYNHPYPTSLVSLDSSLNLGTDTSSSLFFLKNAAGLGLNDSLDLSKKMQHHHDPSLTMAAGPNPMPPLGLPAPQDDTTGTSGDPEDDQSPEEEANAEEDEEDEDEAEEDLNTDSYEDSMPQPDGDNKDNDESFDASLNHTDTSQLEKQEADP

Target ORF information:

RefSeq Version	XM_021616110.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021616110.1

ORF Insert Sequence:

ATGAAAGTTA CATGTCTGAG AGGATTTAAC TTCGACTTCC CTGAATATGC TGAAAGGACT 
TTATGCACTG AAAAGACCTC AGGAAAATCC AACATGGCCG GCAAAAGGAA AGGTGGCCTA 
AAACTCAACG CAATCTGTGC CAAGCTGAGC CGTCAGGTGG TGTACGACGG CAGCTCCCAG 
CATGCTGAGG GAGACCACAG TGTGGCGGAG AACAGCGAGC GCGGGAGCTC CCATTATGAC 
GACAGCGGCG AGCGATTCGA GACCAACTTC CCCGAGGGCC TGAGTCTCGG CCAGAGCCTC 
GAAGAGGACC AGAAACGGCG CAAGGCCATT GAGAAGTGGG TCAACGGGGA GTATGGGGAT 
GAACCACCAC CCTCTGCCCT GGACTGTGGG GAGGAACAGC AGCAACAGCA GGGACTCAAG 
CCCAACAATG GCGAGGACGG ACCCCCCGAG GGTGTGTACA TGGTACAGCC CAAGGGCTGT 
AGTGACGAGG AGGACAATGC GCTTGATGAG CCCGAACCCA TGTTCGACTC TCGGGACGGC 
AGTTACCACG ACGACAAGGA CTCGGAAGAC GGGCCTCAAA AGGACGTCTA CATGCCGTTG 
ATAAGTGAGC CCCCGAGTCG CCAAACATCT TTCTCCTCTC CAGGAGAGGC GTCCACTCTG 
CGAGACTACG CAGCCAACAC AATGAACGAG TTCCTGGGCA TGTTCGGCTA CGACGACCAG 
CAGGTGAGGG ACGAGCTGGC CAAGAAGATC AGCTTCGAGA AGCTCAAAGC CGCTACCTCA 
GGCTCCGACC CATCCTCCCT GAGCAGCGAG GAGGCCACGC GGCGAGCCCG CTTTTCCAAG 
TATGAGGAGT ACATCCGCAA GCTGAAGGCT GGCGAGACCC TGCCCTGGCC CATCCACACC 
AACCCCAAAC AAGAGGACCT CAACTCCAAG CTTGCCCAGG AGAAGAATGC CCTGCTCCAG 
GCCCAGATGG CCGCCTCTGG GGGGTGCCAT TCCGGGGCTG AGGGACAGAT TTATCACTCC 
AGCCTGGACC ACAAGCAGGG GCAAGGGCAG GGGCACAACC TTGGGCACAA TCCCCCACCA 
ACCAACCCCA CCTCCCACCT CCAGAACATG GCCTCCCGAG CCTCTAAGTA TGACTTCTTC 
ATCCAGAAGC TGAAGATGGG TGAAAGCCTG CGGAGCCAGC AGAATGGCAA TGCTTACAAG 
AAGCCATCCA AGTACGACCT GGAGAATGTT CGATACCTGC ACCTCTTCAA GCCCGGCGAC 
GGGAACACTG ACATGGGGGG CGCCATCGCC TATAAAACGG GGAAGGTGGG CCGCCCGTCC 
AAGTATGATA TCAAGAACAT CCAGAAGCTG ATGCCGGGGA GGCCCGAGTC CTCGCTGATG 
CCCAGCGTTC TGCCTGCCAC ACCTGGGAAC CCGGGGACAC CTGTGGCTGC CACCTCGGCC 
TCCTCCGCTG GGGCCACGGG CCCCCCAGGA CTCATCATGG ATCAGACAGG CCACTTGGGC 
TTCAACACCT CCGACTACAT GAAGTCCAGC TTCTCCAAGA CCGACTCCAT CACCACGGGC 
ACTGTGTCTT CTGTCAAGAA CGGCTTACCA CCTGAGAAAC CTGTCACAGA GGACGTCAAC 
CTGTACCAGA AATATATCGC CAGAAGCCGT CAAACCTTCC CGAAGCCCCT ACCACCACCA 
CCACCACCAC CATACCCTGA GCCCCCCACC ACACCACCAA CTTTTAAAAG GTTCTCGGGG 
AGCCAGCACT GCGGACATGT GCATTGTGCC TACCAATACA GGGAGCATTA CCACTGCATG 
GACACGGAGT GCAACTACCA GAGGTTCACC AGCAAGCAAG ACGTGATCAG GCACTACAAT 
ATGCACAAGA AGAGGGACAA CTCCCTACAG CATGGCTTCA TGCGCTTCAG CCCACTGGAC 
GACTGTAGCG TCTACTACCA TGGCTGCCAC CTCAACGGGA AGAGCACCCA CTACCACTGC 
ATGCAGGTGG GCTGCAACAA GGTATACACC AGCACCTCCG ATGTCATGAC TCACGAGAAC 
TTCCATAAGA AGAACGCCCA GCTGATCAAC GATGGCTTTC AGAGGTTCCG CGCCACTGAG 
GATTGTGGGA CGGTGGAGTG CCAATTCTAC GGGCAGAAGA CCACCCACTT CCACTGCAGG 
CGCCCGGGGT GCACCTTCAC CTTCAAGAAC AAGTGCGACA TTGAGAAGCA CAAGAGCTAC 
CACATCAAGG ATGACGCATA TGCCAAGGAT GGCTTCAAGA AGTTCTACAA GTATGAGGAG 
TGCAAATACG AGGGCTGTGT CTACAGCAAG GCCACCAACC ACTTCCACTG CATCCGCTCA 
AGCTGCGGCT TCACCTTCAC CTCCACCAGC CAGATGACCT CCCACAAGCG CAAGCACGAG 
CGCCGCCACA TCCGCTCCTC GGGCGTGATG GCCCTCTCCT CTGGCGGCTC CTTCCTGATG 
CCCAAGGAGG AACCCGGAGA CGAGTCGAGC AACGACGACC TCATGGACTT CTCGGCCATC 
AGCAGCAAGA ACTCCAGCCT GAGCGCCTCG CCCACCACCC AGCAGTCGTC CAACGGTCCC 
GGCACCCACT TGCTTGCCAC ACCCACCACG GTGATGTCGT CCTCCGGCGG TGGCCACAGC 
GGCCTCAAGC CCACCTCCTC GCTGCCGCAC TCGGCACGCA TGTCAAGCCT GCTATCCCAG 
GCGCTGCCCA GCAACATGCC TGTGGCACTG GCGCTGTCCA ACAGTGCCCT CGCCAGCGTC 
AACAATCCCT TCTTCCCGCT CATGCCCCGG CTGCCCATGC AGCTGCCCCA TTCGGCCGCC 
AGCCTGATCT CGGCCGTGTC GTCTGGCGCC CACTCCATGC CCACCGACTC ACTGTCCCAG 
GGCGGCTGCT CCTCGGCGGG CAGCGATGGC GCCATGGCAT CCACGCCGAC CTCCTACGCC 
TCAGCCTCTT CCATCATGGA GAAGATCTCT GCTAGCAAGG GCCTCATATC CCCGATGATG 
GCCAGGCTGG CAGCAGCTGC CCTCAAGCCC TCTGGCAACC ACCAAGACAC AGGGAATGGG 
CTGGCTGGTT CTTCAGCGAG TCTGTTCAAT CTGGTTCAAG TGAAGCAGGA GCCAGTCGAC 
CATACCTCCG GGGCCTCCCA GGACTCCATC CAAGAGCACA GCCTGGACCT GAGCAAAAAA 
GACCACAGTA ATGAATCAAA CGGACACCCT GTACCGGGAA ATACATCTCT TTTATCCTCG 
CTTATGAATA AGATGAATGC CGGCTTCTTC AGTGCCCTGA ACCTGAAGAC GGAGATGGAG 
ATCGGCCAAG GGGCCGACAC CTCAGAGGCA GCACAGTACC TGAGCCGCAT CATGAAGAGG 
CCCATGCCAG ACAAACCAAG CGAGCTCTGG AGGACATATC TGCGCAGGTT TGACACGGAG 
GACTTCTGCG AGGCCCAGTG TGACTTCCTG CAGAAGGTCC ACTTCCATTG TCTAGTGGAG 
GACTGCGGGG CGCTCTTCAG CACGATGGAC GGAGCCATTA AACACGCCAA CTTCCACCTA 
CGAGCCACGC TGAAGGTGAA ATCAGAGCCT CACTTCAACA AAGTCAAGGA CTGCGGCGAC 
GGAGCATCCC ACCAGTCCAC CGCCCCTGTC TCCATGGCTA ACAACCCCGC CATGGACGTG 
GCCAACATGT CATCCTCGTC AGGCGGCGGC TATGGCTCCT CCCCCTCACT GCTGGCATGG 
AAGCAGCTGA CGGGCAGCAT CCCACAGCTC AATGCCTCCA TGCCTAACCT GCCTGCCAAT 
TCGCCGCTGG CCACCACCTC TCTGGAGAAC GCCAAGCCAC AGGTCAAACC GGGCTTCCTG 
CAGTTCCAGG ACAATTCATA CAGTGACCCT TGCCTGGCCA CTGACTGTAA GTACTCCAAC 
AAGGTCCACT TCCACTGTCT CTTCGGGAAC TGCAAGTACG TCTGCAAGAC CTCGGGGAAG 
GCCGAATCCC ACTGCTTGGA CCACATCAAT CCTAACAACC ACCTGGTCAA CGTTCGAGAC 
CAGTTCTCCT ACTTCTCCCT CCAGTGTCTA TGTCCCAACC AGCACTGTGA GTTCAGAATG 
AGGGGCCATT ACCACTGTCT GCGATCCGGC TGCTACTTTG TCACCAACAT CACCACCAAG 
CTTCCCTGGC ATGTCAAGAA GCATGAGAAG GCAGAGAGAC GCGCCGCCAA TGGCTTCAAA 
TACTTCACCA AGAGAGAGGA GTGTGGCAGG CTAGGCTGCA AGTACAACCA GGTGAACAGT 
CACTTCCACT GCATCCGCGA GAGCTGCCAG TTCTCCTTCC TGCTCAAGCA CCAGATGACC 
TCGCACTCCC GCAAACACAT GAGGAGAATG CTGGGAAAGA ACTTTGACAG GGTTCCATCC 
CAGGTGGTGT CCCATGGCCA GAGGGCCGAT GACATGCAGC ACTTGTCGGG CATGGGCTCA 
GGTCTCATGG GCAGCCACCC GGGGATGACC CATGGGTTCT CCAGCATGAT GGATGAGACG 
GATGACTACA TGGACTACAT GGGCGGGGGC AGCCCCCTGT GCCTTTCCTC TGAGTCCTCC 
AATCAGGACC GCAGTTGCAG CAGCACCCCC GTGGGCAATG AAAGCTCCCC AGCAGGCAAC 
ACCATCTCCA TCCCTACGGC CACCGGCGCC AAGAAACGCT TCTGGATCAT CGAGGACATG 
TCCCCCTTCG GCAAGCGGCG TAAGACGGCT TCTGCTCGCA AAATGCTGGA CGAGGGCATG 
ATGCTGGAGG GCTTCCGCCG TTATGATCTG TACGAGGACT GCAAGGACTC CGGCTGCCAG 
TTCTCTCTGA AGGTGACCCA CTACCACTGC ACCCGCGAGA ACTGCGGCTA CAAGTTCTGC 
GGCCGCACGC ACATGTACAA GCATGCGCAG CACCACGACC GCGTGGACAA CCTGGTCCTG 
GACGACTTCA AGCGTTTCAA GTCATCGCTC AGCTGCAACT TCTCCGACTG CCAGTTCTCC 
GGCAACAGCA CCCACTTCCA CTGCCTGCGC TGCAGCTTCC GTTGCACGGA CAGCACCAAG 
GTGACTGCCC ACCGCAAACA CCATGGCAAG CAAGATGTGA TCAGCGCCGC TGGCTTCTGC 
CAGTTCAGCT CCAGCGCTGA CTGCGAGGTT GTCGACTGCA AATACAAGCT CAAGTGCTCA 
CACTTTCACT GCACCTACCC CGAGTGCAAG CACACTGTAG TCGGTATGTC CCAGATGGAC 
TCCCACAAGC GCAAGCATGA GAAGCAGGAG CGCGGCGAGC TGCCGTCGGT GTCTCCCCGT 
CAGGAGGGGG CGCACCACAT GACGGGAGGA GTGTCCGCAA TGCCCCACGC CTCCATGGGC 
CTTCTTCCAG CCTCGCCCGG TGGCCTCCCA GGACTCTCCC ACCACCACCA TCACCACCAT 
AACAACAACA ACCATGCCCC CTCCATGCTC TACCCCCCAG CCAGCGTGGC ATCTGACTAC 
AACCACCCCT ATCCAACCTC CTTGGTCAGT CTGGACAGCT CCCTCAACCT GGGCACCGAC 
ACCAGCAGCT CCCTGTTCTT CCTGAAGAAC GCCGCTGGCC TGGGCCTCAA TGACTCGTTG 
GACCTGAGCA AAAAGATGCA GCATCACCAT GACCCGAGCC TGACCATGGC TGCCGGGCCC 
AACCCCATGC CACCGCTGGG CTTGCCCGCT CCTCAGGATG ACACCACGGG AACATCGGGC 
GACCCCGAAG ATGACCAGTC ACCCGAGGAA GAGGCCAATG CAGAGGAAGA TGAGGAGGAT 
GAGGATGAGG CGGAGGAGGA TCTGAACACT GATTCGTACG AAGATTCGAT GCCACAGCCC 
GACGGCGACA ACAAGGACAA TGACGAGAGT TTTGACGCTT CCTTAAACCA CACTGACACT 
TCCCAACTGG AAAAGCAAGA GGCCGACCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

casz1 ( XM_021616111.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021471786.1 Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X7, mRNA.

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CloneID	OOf07780
Clone ID Related Accession (Same CDS sequence)	XM_021616111.1
Accession Version	XM_021616111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	zinc finger protein castor homolog 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTA CATGTCTGAG AGGATTTAAC TTCGACTTCC CTGAATATGC TGAAAGGACT 
TTATGCACTG AAAAGACCTC AGGAAAATCC AACATGGCCG GCAAAAGGAA AGGTGGCCTA 
AAACTCAACG CAATCTGTGC CAAGCTGAGC CGTCAGGTGG TGTACGACGG CAGCTCCCAG 
CATGCTGAGG GAGACCACAG TGTGGCGGAG AACAGCGAGC GCGGGAGCTC CCATTATGAC 
GACAGCGGCG AGCGATTCGA GACCAACTTC CCCGAGGGCC TGAGTCTCGG CCAGAGCCTC 
GAAGAGGACC AGAAACGGCG CAAGGCCATT GAGAAGTGGG TCAACGGGGA GTATGGGGAT 
GAACCACCAC CCTCTGCCCT GGACTGTGGG GAGGAACAGC AGCAACAGCA GGGACTCAAG 
CCCAACAATG GCGAGGACGG ACCCCCCGAG GGTGTGTACA TGGTACAGCC CAAGGGCTGT 
AGTGACGAGG AGGACAATGC GCTTGATGAG CCCGAACCCA TGTTCGACTC TCGGGACGGC 
AGTTACCACG ACGACAAGGA CTCGGAAGAC GGGCCTCAAA AGGACGTCTA CATGCCGTTG 
ATAAGTGAGC CCCCGAGTCG CCAAACATCT TTCTCCTCTC CAGGAGAGGC GTCCACTCTG 
CGAGACTACG CAGCCAACAC AATGAACGAG TTCCTGGGCA TGTTCGGCTA CGACGACCAG 
CAGGTGAGGG ACGAGCTGGC CAAGAAGATC AGCTTCGAGA AGCTCAAAGC CGCTACCTCA 
GGCTCCGACC CATCCTCCCT GAGCAGCGAG GAGGCCACGC GGCGAGCCCG CTTTTCCAAG 
TATGAGGAGT ACATCCGCAA GCTGAAGGCT GGCGAGACCC TGCCCTGGCC CATCCACACC 
AACCCCAAAC AAGAGGACCT CAACTCCAAG CTTGCCCAGG AGAAGAATGC CCTGCTCCAG 
GCCCAGATGG CCGCCTCTGG GGGGTGCCAT TCCGGGGCTG AGGGACAGAT TTATCACTCC 
AGCCTGGACC ACAAGCAGGG GCAAGGGCAG GGGCACAACC TTGGGCACAA TCCCCCACCA 
ACCAACCCCA CCTCCCACCT CCAGAACATG GCCTCCCGAG CCTCTAAGTA TGACTTCTTC 
ATCCAGAAGC TGAAGATGGG TGAAAGCCTG CGGAGCCAGC AGAATGGCAA TGCTTACAAG 
AAGCCATCCA AGTACGACCT GGAGAATGTT CGATACCTGC ACCTCTTCAA GCCCGGCGAC 
GGGAACACTG ACATGGGGGG CGCCATCGCC TATAAAACGG GGAAGGTGGG CCGCCCGTCC 
AAGTATGATA TCAAGAACAT CCAGAAGCTG ATGCCGGGGA GGCCCGAGTC CTCGCTGATG 
CCCAGCGTTC TGCCTGCCAC ACCTGGGAAC CCGGGGACAC CTGTGGCTGC CACCTCGGCC 
TCCTCCGCTG GGGCCACGGG CCCCCCAGGA CTCATCATGG ATCAGACAGG CCACTTGGGC 
TTCAACACCT CCGACTACAT GAAGTCCAGC TTCTCCAAGA CCGACTCCAT CACCACGGGC 
ACTGTGTCTT CTGTCAAGAA CGGCTTACCA CCTGAGAAAC CTGTCACAGA GGACGTCAAC 
CTGTACCAGA AATATATCGC CAGGTTCTCG GGGAGCCAGC ACTGCGGACA TGTGCATTGT 
GCCTACCAAT ACAGGGAGCA TTACCACTGC ATGGACACGG AGTGCAACTA CCAGAGGTTC 
ACCAGCAAGC AAGACGTGAT CAGGCACTAC AATATGCACA AGAAGAGGGA CAACTCCCTA 
CAGCATGGCT TCATGCGCTT CAGCCCACTG GACGACTGTA GCGTCTACTA CCATGGCTGC 
CACCTCAACG GGAAGAGCAC CCACTACCAC TGCATGCAGG TGGGCTGCAA CAAGGTATAC 
ACCAGCACCT CCGATGTCAT GACTCACGAG AACTTCCATA AGAAGAACGC CCAGCTGATC 
AACGATGGCT TTCAGAGGTT CCGCGCCACT GAGGATTGTG GGACGGTGGA GTGCCAATTC 
TACGGGCAGA AGACCACCCA CTTCCACTGC AGGCGCCCGG GGTGCACCTT CACCTTCAAG 
AACAAGTGCG ACATTGAGAA GCACAAGAGC TACCACATCA AGGATGACGC ATATGCCAAG 
GATGGCTTCA AGAAGTTCTA CAAGTATGAG GAGTGCAAAT ACGAGGGCTG TGTCTACAGC 
AAGGCCACCA ACCACTTCCA CTGCATCCGC TCAAGCTGCG GCTTCACCTT CACCTCCACC 
AGCCAGATGA CCTCCCACAA GCGCAAGCAC GAGCGCCGCC ACATCCGCTC CTCGGGCGTG 
ATGGCCCTCT CCTCTGGCGG CTCCTTCCTG ATGCCCAAGG AGGAACCCGG AGACGAGTCG 
AGCAACGACG ACCTCATGGA CTTCTCGGCC ATCAGCAGCA AGAACTCCAG CCTGAGCGCC 
TCGCCCACCA CCCAGCAGTC GTCCAACGGT CCCGGCACCC ACTTGCTTGC CACACCCACC 
ACGGTGATGT CGTCCTCCGG CGGTGGCCAC AGCGGCCTCA AGCCCACCTC CTCGCTGCCG 
CACTCGGCAC GCATGTCAAG CCTGCTATCC CAGGCGCTGC CCAGCAACAT GCCTGTGGCA 
CTGGCGCTGT CCAACAGTGC CCTCGCCAGC GTCAACAATC CCTTCTTCCC GCTCATGCCC 
CGGCTGCCCA TGCAGCTGCC CCATTCGGCC GCCAGCCTGA TCTCGGCCGT GTCGTCTGGC 
GCCCACTCCA TGCCCACCGA CTCACTGTCC CAGGGCGGCT GCTCCTCGGC GGGCAGCGAT 
GGCGCCATGG CATCCACGCC GACCTCCTAC GCCTCAGCCT CTTCCATCAT GGAGAAGATC 
TCTGCTAGCA AGGGCCTCAT ATCCCCGATG ATGGCCAGGC TGGCAGCAGC TGCCCTCAAG 
CCCTCTGGCA ACCACCAAGA CACAGGGAAT GGGCTGGCTG GTTCTTCAGC GAGTCTGTTC 
AATCTGGTTC AAGTGAAGCA GGAGCCAGTC GACCATACCT CCGGGGCCTC CCAGGACTCC 
ATCCAAGAGC ACAGCCTGGA CCTGAGCAAA AAAGACCACA GTAATGAATC AAACGGACAC 
CCTGTACCGG GAAATACATC TCTTTTATCC TCGCTTATGA ATAAGATGAA TGCCGGCTTC 
TTCAGTGCCC TGAACCTGAA GACGGAGATG GAGATCGGCC AAGGGGCCGA CACCTCAGAG 
GCAGCACAGT ACCTGAGCCG CATCATGAAG AGGCCCATGC CAGACAAACC AAGCGAGCTC 
TGGAGGACAT ATCTGCGCAG GTTTGACACG GAGGACTTCT GCGAGGCCCA GTGTGACTTC 
CTGCAGAAGG TCCACTTCCA TTGTCTAGTG GAGGACTGCG GGGCGCTCTT CAGCACGATG 
GACGGAGCCA TTAAACACGC CAACTTCCAC CTACGAGCCA CGCTGAAGGT GAAATCAGAG 
CCTCACTTCA ACAAAGTCAA GGACTGCGGC GACGGAGCAT CCCACCAGTC CACCGCCCCT 
GTCTCCATGG CTAACAACCC CGCCATGGAC GTGGCCAACA TGTCATCCTC GTCAGGCGGC 
GGCTATGGCT CCTCCCCCTC ACTGCTGGCA TGGAAGCAGC TGACGGGCAG CATCCCACAG 
CTCAATGCCT CCATGCCTAA CCTGCCTGCC AATTCGCCGC TGGCCACCAC CTCTCTGGAG 
AACGCCAAGC CACAGGTCAA ACCGGGCTTC CTGCAGTTCC AGGACAATGA CCCTTGCCTG 
GCCACTGACT GTAAGTACTC CAACAAGGTC CACTTCCACT GTCTCTTCGG GAACTGCAAG 
TACGTCTGCA AGACCTCGGG GAAGGCCGAA TCCCACTGCT TGGACCACAT CAATCCTAAC 
AACCACCTGG TCAACGTTCG AGACCAGTTC TCCTACTTCT CCCTCCAGTG TCTATGTCCC 
AACCAGCACT GTGAGTTCAG AATGAGGGGC CATTACCACT GTCTGCGATC CGGCTGCTAC 
TTTGTCACCA ACATCACCAC CAAGCTTCCC TGGCATGTCA AGAAGCATGA GAAGGCAGAG 
AGACGCGCCG CCAATGGCTT CAAATACTTC ACCAAGAGAG AGGAGTGTGG CAGGCTAGGC 
TGCAAGTACA ACCAGGTGAA CAGTCACTTC CACTGCATCC GCGAGAGCTG CCAGTTCTCC 
TTCCTGCTCA AGCACCAGAT GACCTCGCAC TCCCGCAAAC ACATGAGGAG AATGCTGGGA 
AAGAACTTTG ACAGGGTTCC ATCCCAGGTG GTGTCCCATG GCCAGAGGGC CGATGACATG 
CAGCACTTGT CGGGCATGGG CTCAGGTCTC ATGGGCAGCC ACCCGGGGAT GACCCATGGG 
TTCTCCAGCA TGATGGATGA GACGGATGAC TACATGGACT ACATGGGCGG GGGCAGCCCC 
CTGTGCCTTT CCTCTGAGTC CTCCAATCAG GACCGCAGTT GCAGCAGCAC CCCCGTGGGC 
AATGAAAGCT CCCCAGCAGG CAACACCATC TCCATCCCTA CGGCCACCGG CGCCAAGAAA 
CGCTTCTGGA TCATCGAGGA CATGTCCCCC TTCGGCAAGC GGCGTAAGAC GGCTTCTGCT 
CGCAAAATGC TGGACGAGGG CATGATGCTG GAGGGCTTCC GCCGTTATGA TCTGTACGAG 
GACTGCAAGG ACTCCGGCTG CCAGTTCTCT CTGAAGGTGA CCCACTACCA CTGCACCCGC 
GAGAACTGCG GCTACAAGTT CTGCGGCCGC ACGCACATGT ACAAGCATGC GCAGCACCAC 
GACCGCGTGG ACAACCTGGT CCTGGACGAC TTCAAGCGTT TCAAGTCATC GCTCAGCTGC 
AACTTCTCCG ACTGCCAGTT CTCCGGCAAC AGCACCCACT TCCACTGCCT GCGCTGCAGC 
TTCCGTTGCA CGGACAGCAC CAAGGTGACT GCCCACCGCA AACACCATGG CAAGCAAGAT 
GTGATCAGCG CCGCTGGCTT CTGCCAGTTC AGCTCCAGCG CTGACTGCGA GGTTGTCGAC 
TGCAAATACA AGCTCAAGTG CTCACACTTT CACTGCACCT ACCCCGAGTG CAAGCACACT 
GTAGTCGGTA TGTCCCAGAT GGACTCCCAC AAGCGCAAGC ATGAGAAGCA GGAGCGCGGC 
GAGCTGCCGT CGGTGTCTCC CCGTCAGGAG GGGGCGCACC ACATGACGGG AGGAGTGTCC 
GCAATGCCCC ACGCCTCCAT GGGCCTTCTT CCAGCCTCGC CCGGTGGCCT CCCAGGACTC 
TCCCACCACC ACCATCACCA CCATAACAAC AACAACCATG CCCCCTCCAT GCTCTACCCC 
CCAGCCAGCG TGGCATCTGA CTACAACCAC CCCTATCCAA CCTCCTTGGT CAGTCTGGAC 
AGCTCCCTCA ACCTGGGCAC CGACACCAGC AGCTCCCTGT TCTTCCTGAA GAACGCCGCT 
GGCCTGGGCC TCAATGACTC GTTGGACCTG AGCAAAAAGA TGCAGCATCA CCATGACCCG 
AGCCTGACCA TGGCTGCCGG GCCCAACCCC ATGCCACCGC TGGGCTTGCC CGCTCCTCAG 
GATGACACCA CGGGAACATC GGGCGACCCC GAAGATGACC AGTCACCCGA GGAAGAGGCC 
AATGCAGAGG AAGATGAGGA GGATGAGGAT GAGGCGGAGG AGGATCTGAA CACTGATTCG 
TACGAAGATT CGATGCCACA GCCCGACGGC GACAACAAGG ACAATGACGA GAGTTTTGAC 
GCTTCCTTAA ACCACACTGA CACTTCCCAA CTGGAAAAGC AAGAGGCCGA CCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021471786.1
CDS	355..6171
Translation	MKVTCLRGFNFDFPEYAERTLCTEKTSGKSNMAGKRKGGLKLNAICAKLSRQVVYDGSSQHAEGDHSVAENSERGSSHYDDSGERFETNFPEGLSLGQSLEEDQKRRKAIEKWVNGEYGDEPPPSALDCGEEQQQQQGLKPNNGEDGPPEGVYMVQPKGCSDEEDNALDEPEPMFDSRDGSYHDDKDSEDGPQKDVYMPLISEPPSRQTSFSSPGEASTLRDYAANTMNEFLGMFGYDDQQVRDELAKKISFEKLKAATSGSDPSSLSSEEATRRARFSKYEEYIRKLKAGETLPWPIHTNPKQEDLNSKLAQEKNALLQAQMAASGGCHSGAEGQIYHSSLDHKQGQGQGHNLGHNPPPTNPTSHLQNMASRASKYDFFIQKLKMGESLRSQQNGNAYKKPSKYDLENVRYLHLFKPGDGNTDMGGAIAYKTGKVGRPSKYDIKNIQKLMPGRPESSLMPSVLPATPGNPGTPVAATSASSAGATGPPGLIMDQTGHLGFNTSDYMKSSFSKTDSITTGTVSSVKNGLPPEKPVTEDVNLYQKYIARFSGSQHCGHVHCAYQYREHYHCMDTECNYQRFTSKQDVIRHYNMHKKRDNSLQHGFMRFSPLDDCSVYYHGCHLNGKSTHYHCMQVGCNKVYTSTSDVMTHENFHKKNAQLINDGFQRFRATEDCGTVECQFYGQKTTHFHCRRPGCTFTFKNKCDIEKHKSYHIKDDAYAKDGFKKFYKYEECKYEGCVYSKATNHFHCIRSSCGFTFTSTSQMTSHKRKHERRHIRSSGVMALSSGGSFLMPKEEPGDESSNDDLMDFSAISSKNSSLSASPTTQQSSNGPGTHLLATPTTVMSSSGGGHSGLKPTSSLPHSARMSSLLSQALPSNMPVALALSNSALASVNNPFFPLMPRLPMQLPHSAASLISAVSSGAHSMPTDSLSQGGCSSAGSDGAMASTPTSYASASSIMEKISASKGLISPMMARLAAAALKPSGNHQDTGNGLAGSSASLFNLVQVKQEPVDHTSGASQDSIQEHSLDLSKKDHSNESNGHPVPGNTSLLSSLMNKMNAGFFSALNLKTEMEIGQGADTSEAAQYLSRIMKRPMPDKPSELWRTYLRRFDTEDFCEAQCDFLQKVHFHCLVEDCGALFSTMDGAIKHANFHLRATLKVKSEPHFNKVKDCGDGASHQSTAPVSMANNPAMDVANMSSSSGGGYGSSPSLLAWKQLTGSIPQLNASMPNLPANSPLATTSLENAKPQVKPGFLQFQDNDPCLATDCKYSNKVHFHCLFGNCKYVCKTSGKAESHCLDHINPNNHLVNVRDQFSYFSLQCLCPNQHCEFRMRGHYHCLRSGCYFVTNITTKLPWHVKKHEKAERRAANGFKYFTKREECGRLGCKYNQVNSHFHCIRESCQFSFLLKHQMTSHSRKHMRRMLGKNFDRVPSQVVSHGQRADDMQHLSGMGSGLMGSHPGMTHGFSSMMDETDDYMDYMGGGSPLCLSSESSNQDRSCSSTPVGNESSPAGNTISIPTATGAKKRFWIIEDMSPFGKRRKTASARKMLDEGMMLEGFRRYDLYEDCKDSGCQFSLKVTHYHCTRENCGYKFCGRTHMYKHAQHHDRVDNLVLDDFKRFKSSLSCNFSDCQFSGNSTHFHCLRCSFRCTDSTKVTAHRKHHGKQDVISAAGFCQFSSSADCEVVDCKYKLKCSHFHCTYPECKHTVVGMSQMDSHKRKHEKQERGELPSVSPRQEGAHHMTGGVSAMPHASMGLLPASPGGLPGLSHHHHHHHNNNNHAPSMLYPPASVASDYNHPYPTSLVSLDSSLNLGTDTSSSLFFLKNAAGLGLNDSLDLSKKMQHHHDPSLTMAAGPNPMPPLGLPAPQDDTTGTSGDPEDDQSPEEEANAEEDEEDEDEAEEDLNTDSYEDSMPQPDGDNKDNDESFDASLNHTDTSQLEKQEADP

Target ORF information:

RefSeq Version	XM_021616111.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021616111.1

ORF Insert Sequence:

ATGAAAGTTA CATGTCTGAG AGGATTTAAC TTCGACTTCC CTGAATATGC TGAAAGGACT 
TTATGCACTG AAAAGACCTC AGGAAAATCC AACATGGCCG GCAAAAGGAA AGGTGGCCTA 
AAACTCAACG CAATCTGTGC CAAGCTGAGC CGTCAGGTGG TGTACGACGG CAGCTCCCAG 
CATGCTGAGG GAGACCACAG TGTGGCGGAG AACAGCGAGC GCGGGAGCTC CCATTATGAC 
GACAGCGGCG AGCGATTCGA GACCAACTTC CCCGAGGGCC TGAGTCTCGG CCAGAGCCTC 
GAAGAGGACC AGAAACGGCG CAAGGCCATT GAGAAGTGGG TCAACGGGGA GTATGGGGAT 
GAACCACCAC CCTCTGCCCT GGACTGTGGG GAGGAACAGC AGCAACAGCA GGGACTCAAG 
CCCAACAATG GCGAGGACGG ACCCCCCGAG GGTGTGTACA TGGTACAGCC CAAGGGCTGT 
AGTGACGAGG AGGACAATGC GCTTGATGAG CCCGAACCCA TGTTCGACTC TCGGGACGGC 
AGTTACCACG ACGACAAGGA CTCGGAAGAC GGGCCTCAAA AGGACGTCTA CATGCCGTTG 
ATAAGTGAGC CCCCGAGTCG CCAAACATCT TTCTCCTCTC CAGGAGAGGC GTCCACTCTG 
CGAGACTACG CAGCCAACAC AATGAACGAG TTCCTGGGCA TGTTCGGCTA CGACGACCAG 
CAGGTGAGGG ACGAGCTGGC CAAGAAGATC AGCTTCGAGA AGCTCAAAGC CGCTACCTCA 
GGCTCCGACC CATCCTCCCT GAGCAGCGAG GAGGCCACGC GGCGAGCCCG CTTTTCCAAG 
TATGAGGAGT ACATCCGCAA GCTGAAGGCT GGCGAGACCC TGCCCTGGCC CATCCACACC 
AACCCCAAAC AAGAGGACCT CAACTCCAAG CTTGCCCAGG AGAAGAATGC CCTGCTCCAG 
GCCCAGATGG CCGCCTCTGG GGGGTGCCAT TCCGGGGCTG AGGGACAGAT TTATCACTCC 
AGCCTGGACC ACAAGCAGGG GCAAGGGCAG GGGCACAACC TTGGGCACAA TCCCCCACCA 
ACCAACCCCA CCTCCCACCT CCAGAACATG GCCTCCCGAG CCTCTAAGTA TGACTTCTTC 
ATCCAGAAGC TGAAGATGGG TGAAAGCCTG CGGAGCCAGC AGAATGGCAA TGCTTACAAG 
AAGCCATCCA AGTACGACCT GGAGAATGTT CGATACCTGC ACCTCTTCAA GCCCGGCGAC 
GGGAACACTG ACATGGGGGG CGCCATCGCC TATAAAACGG GGAAGGTGGG CCGCCCGTCC 
AAGTATGATA TCAAGAACAT CCAGAAGCTG ATGCCGGGGA GGCCCGAGTC CTCGCTGATG 
CCCAGCGTTC TGCCTGCCAC ACCTGGGAAC CCGGGGACAC CTGTGGCTGC CACCTCGGCC 
TCCTCCGCTG GGGCCACGGG CCCCCCAGGA CTCATCATGG ATCAGACAGG CCACTTGGGC 
TTCAACACCT CCGACTACAT GAAGTCCAGC TTCTCCAAGA CCGACTCCAT CACCACGGGC 
ACTGTGTCTT CTGTCAAGAA CGGCTTACCA CCTGAGAAAC CTGTCACAGA GGACGTCAAC 
CTGTACCAGA AATATATCGC CAGGTTCTCG GGGAGCCAGC ACTGCGGACA TGTGCATTGT 
GCCTACCAAT ACAGGGAGCA TTACCACTGC ATGGACACGG AGTGCAACTA CCAGAGGTTC 
ACCAGCAAGC AAGACGTGAT CAGGCACTAC AATATGCACA AGAAGAGGGA CAACTCCCTA 
CAGCATGGCT TCATGCGCTT CAGCCCACTG GACGACTGTA GCGTCTACTA CCATGGCTGC 
CACCTCAACG GGAAGAGCAC CCACTACCAC TGCATGCAGG TGGGCTGCAA CAAGGTATAC 
ACCAGCACCT CCGATGTCAT GACTCACGAG AACTTCCATA AGAAGAACGC CCAGCTGATC 
AACGATGGCT TTCAGAGGTT CCGCGCCACT GAGGATTGTG GGACGGTGGA GTGCCAATTC 
TACGGGCAGA AGACCACCCA CTTCCACTGC AGGCGCCCGG GGTGCACCTT CACCTTCAAG 
AACAAGTGCG ACATTGAGAA GCACAAGAGC TACCACATCA AGGATGACGC ATATGCCAAG 
GATGGCTTCA AGAAGTTCTA CAAGTATGAG GAGTGCAAAT ACGAGGGCTG TGTCTACAGC 
AAGGCCACCA ACCACTTCCA CTGCATCCGC TCAAGCTGCG GCTTCACCTT CACCTCCACC 
AGCCAGATGA CCTCCCACAA GCGCAAGCAC GAGCGCCGCC ACATCCGCTC CTCGGGCGTG 
ATGGCCCTCT CCTCTGGCGG CTCCTTCCTG ATGCCCAAGG AGGAACCCGG AGACGAGTCG 
AGCAACGACG ACCTCATGGA CTTCTCGGCC ATCAGCAGCA AGAACTCCAG CCTGAGCGCC 
TCGCCCACCA CCCAGCAGTC GTCCAACGGT CCCGGCACCC ACTTGCTTGC CACACCCACC 
ACGGTGATGT CGTCCTCCGG CGGTGGCCAC AGCGGCCTCA AGCCCACCTC CTCGCTGCCG 
CACTCGGCAC GCATGTCAAG CCTGCTATCC CAGGCGCTGC CCAGCAACAT GCCTGTGGCA 
CTGGCGCTGT CCAACAGTGC CCTCGCCAGC GTCAACAATC CCTTCTTCCC GCTCATGCCC 
CGGCTGCCCA TGCAGCTGCC CCATTCGGCC GCCAGCCTGA TCTCGGCCGT GTCGTCTGGC 
GCCCACTCCA TGCCCACCGA CTCACTGTCC CAGGGCGGCT GCTCCTCGGC GGGCAGCGAT 
GGCGCCATGG CATCCACGCC GACCTCCTAC GCCTCAGCCT CTTCCATCAT GGAGAAGATC 
TCTGCTAGCA AGGGCCTCAT ATCCCCGATG ATGGCCAGGC TGGCAGCAGC TGCCCTCAAG 
CCCTCTGGCA ACCACCAAGA CACAGGGAAT GGGCTGGCTG GTTCTTCAGC GAGTCTGTTC 
AATCTGGTTC AAGTGAAGCA GGAGCCAGTC GACCATACCT CCGGGGCCTC CCAGGACTCC 
ATCCAAGAGC ACAGCCTGGA CCTGAGCAAA AAAGACCACA GTAATGAATC AAACGGACAC 
CCTGTACCGG GAAATACATC TCTTTTATCC TCGCTTATGA ATAAGATGAA TGCCGGCTTC 
TTCAGTGCCC TGAACCTGAA GACGGAGATG GAGATCGGCC AAGGGGCCGA CACCTCAGAG 
GCAGCACAGT ACCTGAGCCG CATCATGAAG AGGCCCATGC CAGACAAACC AAGCGAGCTC 
TGGAGGACAT ATCTGCGCAG GTTTGACACG GAGGACTTCT GCGAGGCCCA GTGTGACTTC 
CTGCAGAAGG TCCACTTCCA TTGTCTAGTG GAGGACTGCG GGGCGCTCTT CAGCACGATG 
GACGGAGCCA TTAAACACGC CAACTTCCAC CTACGAGCCA CGCTGAAGGT GAAATCAGAG 
CCTCACTTCA ACAAAGTCAA GGACTGCGGC GACGGAGCAT CCCACCAGTC CACCGCCCCT 
GTCTCCATGG CTAACAACCC CGCCATGGAC GTGGCCAACA TGTCATCCTC GTCAGGCGGC 
GGCTATGGCT CCTCCCCCTC ACTGCTGGCA TGGAAGCAGC TGACGGGCAG CATCCCACAG 
CTCAATGCCT CCATGCCTAA CCTGCCTGCC AATTCGCCGC TGGCCACCAC CTCTCTGGAG 
AACGCCAAGC CACAGGTCAA ACCGGGCTTC CTGCAGTTCC AGGACAATGA CCCTTGCCTG 
GCCACTGACT GTAAGTACTC CAACAAGGTC CACTTCCACT GTCTCTTCGG GAACTGCAAG 
TACGTCTGCA AGACCTCGGG GAAGGCCGAA TCCCACTGCT TGGACCACAT CAATCCTAAC 
AACCACCTGG TCAACGTTCG AGACCAGTTC TCCTACTTCT CCCTCCAGTG TCTATGTCCC 
AACCAGCACT GTGAGTTCAG AATGAGGGGC CATTACCACT GTCTGCGATC CGGCTGCTAC 
TTTGTCACCA ACATCACCAC CAAGCTTCCC TGGCATGTCA AGAAGCATGA GAAGGCAGAG 
AGACGCGCCG CCAATGGCTT CAAATACTTC ACCAAGAGAG AGGAGTGTGG CAGGCTAGGC 
TGCAAGTACA ACCAGGTGAA CAGTCACTTC CACTGCATCC GCGAGAGCTG CCAGTTCTCC 
TTCCTGCTCA AGCACCAGAT GACCTCGCAC TCCCGCAAAC ACATGAGGAG AATGCTGGGA 
AAGAACTTTG ACAGGGTTCC ATCCCAGGTG GTGTCCCATG GCCAGAGGGC CGATGACATG 
CAGCACTTGT CGGGCATGGG CTCAGGTCTC ATGGGCAGCC ACCCGGGGAT GACCCATGGG 
TTCTCCAGCA TGATGGATGA GACGGATGAC TACATGGACT ACATGGGCGG GGGCAGCCCC 
CTGTGCCTTT CCTCTGAGTC CTCCAATCAG GACCGCAGTT GCAGCAGCAC CCCCGTGGGC 
AATGAAAGCT CCCCAGCAGG CAACACCATC TCCATCCCTA CGGCCACCGG CGCCAAGAAA 
CGCTTCTGGA TCATCGAGGA CATGTCCCCC TTCGGCAAGC GGCGTAAGAC GGCTTCTGCT 
CGCAAAATGC TGGACGAGGG CATGATGCTG GAGGGCTTCC GCCGTTATGA TCTGTACGAG 
GACTGCAAGG ACTCCGGCTG CCAGTTCTCT CTGAAGGTGA CCCACTACCA CTGCACCCGC 
GAGAACTGCG GCTACAAGTT CTGCGGCCGC ACGCACATGT ACAAGCATGC GCAGCACCAC 
GACCGCGTGG ACAACCTGGT CCTGGACGAC TTCAAGCGTT TCAAGTCATC GCTCAGCTGC 
AACTTCTCCG ACTGCCAGTT CTCCGGCAAC AGCACCCACT TCCACTGCCT GCGCTGCAGC 
TTCCGTTGCA CGGACAGCAC CAAGGTGACT GCCCACCGCA AACACCATGG CAAGCAAGAT 
GTGATCAGCG CCGCTGGCTT CTGCCAGTTC AGCTCCAGCG CTGACTGCGA GGTTGTCGAC 
TGCAAATACA AGCTCAAGTG CTCACACTTT CACTGCACCT ACCCCGAGTG CAAGCACACT 
GTAGTCGGTA TGTCCCAGAT GGACTCCCAC AAGCGCAAGC ATGAGAAGCA GGAGCGCGGC 
GAGCTGCCGT CGGTGTCTCC CCGTCAGGAG GGGGCGCACC ACATGACGGG AGGAGTGTCC 
GCAATGCCCC ACGCCTCCAT GGGCCTTCTT CCAGCCTCGC CCGGTGGCCT CCCAGGACTC 
TCCCACCACC ACCATCACCA CCATAACAAC AACAACCATG CCCCCTCCAT GCTCTACCCC 
CCAGCCAGCG TGGCATCTGA CTACAACCAC CCCTATCCAA CCTCCTTGGT CAGTCTGGAC 
AGCTCCCTCA ACCTGGGCAC CGACACCAGC AGCTCCCTGT TCTTCCTGAA GAACGCCGCT 
GGCCTGGGCC TCAATGACTC GTTGGACCTG AGCAAAAAGA TGCAGCATCA CCATGACCCG 
AGCCTGACCA TGGCTGCCGG GCCCAACCCC ATGCCACCGC TGGGCTTGCC CGCTCCTCAG 
GATGACACCA CGGGAACATC GGGCGACCCC GAAGATGACC AGTCACCCGA GGAAGAGGCC 
AATGCAGAGG AAGATGAGGA GGATGAGGAT GAGGCGGAGG AGGATCTGAA CACTGATTCG 
TACGAAGATT CGATGCCACA GCCCGACGGC GACAACAAGG ACAATGACGA GAGTTTTGAC 
GCTTCCTTAA ACCACACTGA CACTTCCCAA CTGGAAAAGC AAGAGGCCGA CCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

casz1 ( XM_021616105.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021471780.1 Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X1, mRNA.

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CloneID	OOf07774
Clone ID Related Accession (Same CDS sequence)	XM_021616105.1
Accession Version	XM_021616105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	zinc finger protein castor homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTA CATGTCTGAG AGGATTTAAC TTCGACTTCC CTGAATATGC TGAAAGGACT 
TTATGCACTG AAAAGACCTC AGGAAAATCC AACATGGCCG GCAAAAGGAA AGGTGGCCTA 
AAACTCAACG CAATCTGTGC CAAGCTGAGC CGTCAGGTGG TGTACGACGG CAGCTCCCAG 
CATGCTGAGG GAGACCACAG TGTGGCGGAG AACAGCGAGC GCGGGAGCTC CCATTATGAC 
GACAGCGGCG AGCGATTCGA GACCAACTTC CCCGAGGGCC TGAGTCTCGG CCAGAGCCTC 
GAAGAGGACC AGAAACGGCG CAAGGCCATT GAGAAGTGGG TCAACGGGGA GTATGGGGAT 
GAACCACCAC CCTCTGCCCT GGACTGTGGG GAGGAACAGC AGCAACAGCA GGGACTCAAG 
CCCAACAATG GCGAGGACGG ACCCCCCGAG GGTGTGTACA TGGTACAGCC CAAGGGCTGT 
AGTGACGAGG AGGACAATGC GCTTGATGAG CCCGAACCCA TGTTCGACTC TCGGGACGGC 
AGTTACCACG ACGACAAGGA CTCGGAAGAC GGGCCTCAAA AGGACGTCTA CATGCCGTTG 
ATAAGTGAGC CCCCGAGTCG CCAAACATCT TTCTCCTCTC CAGGAGAGGC GTCCACTCTG 
CGAGACTACG CAGCCAACAC AATGAACGAG TTCCTGGGCA TGTTCGGCTA CGACGACCAG 
CAGGTGAGGG ACGAGCTGGC CAAGAAGATC AGCTTCGAGA AGCTCAAAGC CGCTACCTCA 
GGCTCCGACC CATCCTCCCT GAGCAGCGAG GAGGCCACGC GGCGAGCCCG CTTTTCCAAG 
TATGAGGAGT ACATCCGCAA GCTGAAGGCT GGCGAGACCC TGCCCTGGCC CATCCACACC 
AACCCCAAAC AAGAGGACCT CAACTCCAAG CTTGCCCAGG AGAAGAATGC CCTGCTCCAG 
GCCCAGATGG CCGCCTCTGG GGGGTGCCAT TCCGGGGCTG AGGGACAGAT TTATCACTCC 
AGCCTGGACC ACAAGCAGGG GCAAGGGCAG GGGCACAACC TTGGGCACAA TCCCCCACCA 
ACCAACCCCA CCTCCCACCT CCAGAACATG GCCTCCCGAG CCTCTAAGTA TGACTTCTTC 
ATCCAGAAGC TGAAGATGGG TGAAAGCCTG CGGAGCCAGC AGAATGGCAA TGCTTACAAG 
AAGCCATCCA AGTACGACCT GGAGAATGTT CGATACCTGC ACCTCTTCAA GCCCGGCGAC 
GGGAACACTG ACATGGGGGG CGCCATCGCC TATAAAACGG GGAAGGTGGG CCGCCCGTCC 
AAGTATGATA TCAAGAACAT CCAGAAGCTG ATGCCGGGGA GGCCCGAGTC CTCGCTGATG 
CCCAGCGTTC TGCCTGCCAC ACCTGGGAAC CCGGGGACAC CTGTGGCTGC CACCTCGGCC 
TCCTCCGCTG GGGCCACGGG CCCCCCAGGA CTCATCATGG ATCAGACAGG CCACTTGGGC 
TTCAACACCT CCGACTACAT GAAGTCCAGC TTCTCCAAGA CCGACTCCAT CACCACGGGC 
ACTGTGTCTT CTGTCAAGAA CGGCTTACCA CCTGAGAAAC CTGTCACAGA GGACGTCAAC 
CTGTACCAGA AATATATCGC CAGAAGCCGT CAAACCTTCC CGAAGCCCCT ACCACCACCA 
CCACCACCAC CATACCCTGA GCCCCCCACC ACACCACCAA CTTTTAAAAG GTTCTCGGGG 
AGCCAGCACT GCGGACATGT GCATTGTGCC TACCAATACA GGGAGCATTA CCACTGCATG 
GACACGGAGT GCAACTACCA GAGGTTCACC AGCAAGCAAG ACGTGATCAG GCACTACAAT 
ATGCACAAGA AGAGGGACAA CTCCCTACAG CATGGCTTCA TGCGCTTCAG CCCACTGGAC 
GACTGTAGCG TCTACTACCA TGGCTGCCAC CTCAACGGGA AGAGCACCCA CTACCACTGC 
ATGCAGGTGG GCTGCAACAA GGTATACACC AGCACCTCCG ATGTCATGAC TCACGAGAAC 
TTCCATAAGA AGAACGCCCA GCTGATCAAC GATGGCTTTC AGAGGTTCCG CGCCACTGAG 
GATTGTGGGA CGGTGGAGTG CCAATTCTAC GGGCAGAAGA CCACCCACTT CCACTGCAGG 
CGCCCGGGGT GCACCTTCAC CTTCAAGAAC AAGTGCGACA TTGAGAAGCA CAAGAGCTAC 
CACATCAAGG ATGACGCATA TGCCAAGGAT GGCTTCAAGA AGTTCTACAA GTATGAGGAG 
TGCAAATACG AGGGCTGTGT CTACAGCAAG GCCACCAACC ACTTCCACTG CATCCGCTCA 
AGCTGCGGCT TCACCTTCAC CTCCACCAGC CAGATGACCT CCCACAAGCG CAAGCACGAG 
CGCCGCCACA TCCGCTCCTC GGGCGTGATG GCCCTCTCCT CTGGCGGCTC CTTCCTGATG 
CCCAAGGAGG AACCCGGAGA CGAGTCGAGC AACGACGACC TCATGGACTT CTCGGCCATC 
AGCAGCAAGA ACTCCAGCCT GAGCGCCTCG CCCACCACCC AGCAGTCGTC CAACGGTCCC 
GGCACCCACT TGCTTGCCAC ACCCACCACG GTGATGTCGT CCTCCGGCGG TGGCCACAGC 
GGCCTCAAGC CCACCTCCTC GCTGCCGCAC TCGGCACGCA TGTCAAGCCT GCTATCCCAG 
GCGCTGCCCA GCAACATGCC TGTGGCACTG GCGCTGTCCA ACAGTGCCCT CGCCAGCGTC 
AACAATCCCT TCTTCCCGCT CATGCCCCGG CTGCCCATGC AGCTGCCCCA TTCGGCCGCC 
AGCCTGATCT CGGCCGTGTC GTCTGGCGCC CACTCCATGC CCACCGACTC ACTGTCCCAG 
GGCGGCTGCT CCTCGGCGGG CAGCGATGGC GCCATGGCAT CCACGCCGAC CTCCTACGCC 
TCAGCCTCTT CCATCATGGA GAAGATCTCT GCTAGCAAGG GCCTCATATC CCCGATGATG 
GCCAGGCTGG CAGCAGCTGC CCTCAAGCCC TCTGGCAACC ACCAAGACAC AGGGAATGGG 
CTGGCTGGTT CTTCAGCGAG TCTGTTCAAT CTGGTTCAAG TGAAGCAGGA GCCAGTCGAC 
CATACCTCCG GGGCCTCCCA GGACTCCATC CAAGAGCACA GCCTGGACCT GAGCAAAAAA 
GACCACAGTA ATGAATCAAA CGGACACCCT GTACCGGGAA ATACATCTCT TTTATCCTCG 
CTTATGAATA AGATGAATGC CGGCTTCTTC AGTGCCCTGA ACCTGAAGAC GGAGATGGAG 
ATCGGCCAAG GGGCCGACAC CTCAGAGGCA GCACAGTACC TGAGCCGCAT CATGAAGAGG 
CCCATGCCAG ACAAACCAAG CGAGCTCTGG AGGACATATC TGCGCAGGTT TGACACGGAG 
GACTTCTGCG AGGCCCAGTG TGACTTCCTG CAGAAGGTCC ACTTCCATTG TCTAGTGGAG 
GACTGCGGGG CGCTCTTCAG CACGATGGAC GGAGCCATTA AACACGCCAA CTTCCACCTA 
CGAGCCACGC TGAAGGTGAA ATCAGAGCCT CACTTCAACA AAGTCAAGGA CTGCGGCGAC 
GGAGCATCCC ACCAGTCCAC CGCCCCTGTC TCCATGGCTA ACAACCCCGC CATGGACGTG 
GCCAACATGT CATCCTCGTC AGGCGGCGGC TATGGCTCCT CCCCCTCACT GCTGGCATGG 
AAGCAGCTGA CGGGCAGCAT CCCACAGCTC AATGCCTCCA TGCCTAACCT GCCTGCCAAT 
TCGCCGCTGG CCACCACCTC TCTGGAGAAC GCCAAGCCAC AGGTCAAACC GGGCTTCCTG 
CAGTTCCAGG ACAATTCATA CAGTGACCCT TGCCTGGCCA CTGACTGTAA GTACTCCAAC 
AAGGTCCACT TCCACTGTCT CTTCGGGAAC TGCAAGTACG TCTGCAAGAC CTCGGGGAAG 
GCCGAATCCC ACTGCTTGGA CCACATCAAT CCTAACAACC ACCTGGTCAA CGTTCGAGAC 
CAGTTCTCCT ACTTCTCCCT CCAGTGTCTA TGTCCCAACC AGCACTGTGA GTTCAGAATG 
AGGGGCCATT ACCACTGTCT GCGATCCGGC TGCTACTTTG TCACCAACAT CACCACCAAG 
CTTCCCTGGC ATGTCAAGAA GCATGAGAAG GCAGAGAGAC GCGCCGCCAA TGGCTTCAAA 
TACTTCACCA AGAGAGAGGA GTGTGGCAGG CTAGGCTGCA AGTACAACCA GGTGAACAGT 
CACTTCCACT GCATCCGCGA GAGCTGCCAG TTCTCCTTCC TGCTCAAGCA CCAGATGACC 
TCGCACTCCC GCAAACACAT GAGGAGAATG CTGGGAAAGA ACTTTGACAG GGTTCCATCC 
CAGGTGGTGT CCCATGGCCA GAGGGCCGAT GACATGCAGC ACTTGTCGGG CATGGGCTCA 
GGTCTCATGG GCAGCCACCC GGGGATGACC CATGGGTTCT CCAGCATGAT GGATGAGACG 
GATGACTACA TGGACTACAT GGGCGGGGGC AGCCCCCTGT GCCTTTCCTC TGAGTCCTCC 
AATCAGGACC GCAGTTGCAG CAGCACCCCC GTGGGCAATG AAAGCTCCCC AGCAGGACCA 
GGCTGCCCGG TCACTACTGC TCCCTCTACC CCCGCTGACA CTAGCGCTCC CCAAAACGCA 
CCTCCTCCAC CACCTCCTCC TCAGCCTGGA CTTCAGTCTC AGGCTCCATC TTTCCCTCCT 
GCCCTCCTCC GACCTCCTCT TCCCTCGCTC CCATATCTCG TCTCTCCATC CTGTCTGTCA 
TACTCTCTGC TCAGCGCCTC TCTCGGAGCC ACTCGGAGTG TTGTCATGCC AACCAACACA 
CCAGCTTTCA GCCCCATCAT TGCCACTCCG TCTCCGGTTA AAAATGACGT CCCTATAGTG 
CAGGATGCTG CAGGCAACAC CATCTCCATC CCTACGGCCA CCGGCGCCAA GAAACGCTTC 
TGGATCATCG AGGACATGTC CCCCTTCGGC AAGCGGCGTA AGACGGCTTC TGCTCGCAAA 
ATGCTGGACG AGGGCATGAT GCTGGAGGGC TTCCGCCGTT ATGATCTGTA CGAGGACTGC 
AAGGACTCCG GCTGCCAGTT CTCTCTGAAG GTGACCCACT ACCACTGCAC CCGCGAGAAC 
TGCGGCTACA AGTTCTGCGG CCGCACGCAC ATGTACAAGC ATGCGCAGCA CCACGACCGC 
GTGGACAACC TGGTCCTGGA CGACTTCAAG CGTTTCAAGT CATCGCTCAG CTGCAACTTC 
TCCGACTGCC AGTTCTCCGG CAACAGCACC CACTTCCACT GCCTGCGCTG CAGCTTCCGT 
TGCACGGACA GCACCAAGGT GACTGCCCAC CGCAAACACC ATGGCAAGCA AGATGTGATC 
AGCGCCGCTG GCTTCTGCCA GTTCAGCTCC AGCGCTGACT GCGAGGTTGT CGACTGCAAA 
TACAAGCTCA AGTGCTCACA CTTTCACTGC ACCTACCCCG AGTGCAAGCA CACTGTAGTC 
GGTATGTCCC AGATGGACTC CCACAAGCGC AAGCATGAGA AGCAGGAGCG CGGCGAGCTG 
CCGTCGGTGT CTCCCCGTCA GGAGGGGGCG CACCACATGA CGGGAGGAGT GTCCGCAATG 
CCCCACGCCT CCATGGGCCT TCTTCCAGCC TCGCCCGGTG GCCTCCCAGG ACTCTCCCAC 
CACCACCATC ACCACCATAA CAACAACAAC CATGCCCCCT CCATGCTCTA CCCCCCAGCC 
AGCGTGGCAT CTGACTACAA CCACCCCTAT CCAACCTCCT TGGTCAGTCT GGACAGCTCC 
CTCAACCTGG GCACCGACAC CAGCAGCTCC CTGTTCTTCC TGAAGAACGC CGCTGGCCTG 
GGCCTCAATG ACTCGTTGGA CCTGAGCAAA AAGATGCAGC ATCACCATGA CCCGAGCCTG 
ACCATGGCTG CCGGGCCCAA CCCCATGCCA CCGCTGGGCT TGCCCGCTCC TCAGGATGAC 
ACCACGGGAA CATCGGGCGA CCCCGAAGAT GACCAGTCAC CCGAGGAAGA GGCCAATGCA 
GAGGAAGATG AGGAGGATGA GGATGAGGCG GAGGAGGATC TGAACACTGA TTCGTACGAA 
GATTCGATGC CACAGCCCGA CGGCGACAAC AAGGACAATG ACGAGAGTTT TGACGCTTCC 
TTAAACCACA CTGACACTTC CCAACTGGAA AAGCAAGAGG CCGACCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021471780.1
CDS	355..6585
Translation	MKVTCLRGFNFDFPEYAERTLCTEKTSGKSNMAGKRKGGLKLNAICAKLSRQVVYDGSSQHAEGDHSVAENSERGSSHYDDSGERFETNFPEGLSLGQSLEEDQKRRKAIEKWVNGEYGDEPPPSALDCGEEQQQQQGLKPNNGEDGPPEGVYMVQPKGCSDEEDNALDEPEPMFDSRDGSYHDDKDSEDGPQKDVYMPLISEPPSRQTSFSSPGEASTLRDYAANTMNEFLGMFGYDDQQVRDELAKKISFEKLKAATSGSDPSSLSSEEATRRARFSKYEEYIRKLKAGETLPWPIHTNPKQEDLNSKLAQEKNALLQAQMAASGGCHSGAEGQIYHSSLDHKQGQGQGHNLGHNPPPTNPTSHLQNMASRASKYDFFIQKLKMGESLRSQQNGNAYKKPSKYDLENVRYLHLFKPGDGNTDMGGAIAYKTGKVGRPSKYDIKNIQKLMPGRPESSLMPSVLPATPGNPGTPVAATSASSAGATGPPGLIMDQTGHLGFNTSDYMKSSFSKTDSITTGTVSSVKNGLPPEKPVTEDVNLYQKYIARSRQTFPKPLPPPPPPPYPEPPTTPPTFKRFSGSQHCGHVHCAYQYREHYHCMDTECNYQRFTSKQDVIRHYNMHKKRDNSLQHGFMRFSPLDDCSVYYHGCHLNGKSTHYHCMQVGCNKVYTSTSDVMTHENFHKKNAQLINDGFQRFRATEDCGTVECQFYGQKTTHFHCRRPGCTFTFKNKCDIEKHKSYHIKDDAYAKDGFKKFYKYEECKYEGCVYSKATNHFHCIRSSCGFTFTSTSQMTSHKRKHERRHIRSSGVMALSSGGSFLMPKEEPGDESSNDDLMDFSAISSKNSSLSASPTTQQSSNGPGTHLLATPTTVMSSSGGGHSGLKPTSSLPHSARMSSLLSQALPSNMPVALALSNSALASVNNPFFPLMPRLPMQLPHSAASLISAVSSGAHSMPTDSLSQGGCSSAGSDGAMASTPTSYASASSIMEKISASKGLISPMMARLAAAALKPSGNHQDTGNGLAGSSASLFNLVQVKQEPVDHTSGASQDSIQEHSLDLSKKDHSNESNGHPVPGNTSLLSSLMNKMNAGFFSALNLKTEMEIGQGADTSEAAQYLSRIMKRPMPDKPSELWRTYLRRFDTEDFCEAQCDFLQKVHFHCLVEDCGALFSTMDGAIKHANFHLRATLKVKSEPHFNKVKDCGDGASHQSTAPVSMANNPAMDVANMSSSSGGGYGSSPSLLAWKQLTGSIPQLNASMPNLPANSPLATTSLENAKPQVKPGFLQFQDNSYSDPCLATDCKYSNKVHFHCLFGNCKYVCKTSGKAESHCLDHINPNNHLVNVRDQFSYFSLQCLCPNQHCEFRMRGHYHCLRSGCYFVTNITTKLPWHVKKHEKAERRAANGFKYFTKREECGRLGCKYNQVNSHFHCIRESCQFSFLLKHQMTSHSRKHMRRMLGKNFDRVPSQVVSHGQRADDMQHLSGMGSGLMGSHPGMTHGFSSMMDETDDYMDYMGGGSPLCLSSESSNQDRSCSSTPVGNESSPAGPGCPVTTAPSTPADTSAPQNAPPPPPPPQPGLQSQAPSFPPALLRPPLPSLPYLVSPSCLSYSLLSASLGATRSVVMPTNTPAFSPIIATPSPVKNDVPIVQDAAGNTISIPTATGAKKRFWIIEDMSPFGKRRKTASARKMLDEGMMLEGFRRYDLYEDCKDSGCQFSLKVTHYHCTRENCGYKFCGRTHMYKHAQHHDRVDNLVLDDFKRFKSSLSCNFSDCQFSGNSTHFHCLRCSFRCTDSTKVTAHRKHHGKQDVISAAGFCQFSSSADCEVVDCKYKLKCSHFHCTYPECKHTVVGMSQMDSHKRKHEKQERGELPSVSPRQEGAHHMTGGVSAMPHASMGLLPASPGGLPGLSHHHHHHHNNNNHAPSMLYPPASVASDYNHPYPTSLVSLDSSLNLGTDTSSSLFFLKNAAGLGLNDSLDLSKKMQHHHDPSLTMAAGPNPMPPLGLPAPQDDTTGTSGDPEDDQSPEEEANAEEDEEDEDEAEEDLNTDSYEDSMPQPDGDNKDNDESFDASLNHTDTSQLEKQEADP

Target ORF information:

RefSeq Version	XM_021616105.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021616105.1

ORF Insert Sequence:

ATGAAAGTTA CATGTCTGAG AGGATTTAAC TTCGACTTCC CTGAATATGC TGAAAGGACT 
TTATGCACTG AAAAGACCTC AGGAAAATCC AACATGGCCG GCAAAAGGAA AGGTGGCCTA 
AAACTCAACG CAATCTGTGC CAAGCTGAGC CGTCAGGTGG TGTACGACGG CAGCTCCCAG 
CATGCTGAGG GAGACCACAG TGTGGCGGAG AACAGCGAGC GCGGGAGCTC CCATTATGAC 
GACAGCGGCG AGCGATTCGA GACCAACTTC CCCGAGGGCC TGAGTCTCGG CCAGAGCCTC 
GAAGAGGACC AGAAACGGCG CAAGGCCATT GAGAAGTGGG TCAACGGGGA GTATGGGGAT 
GAACCACCAC CCTCTGCCCT GGACTGTGGG GAGGAACAGC AGCAACAGCA GGGACTCAAG 
CCCAACAATG GCGAGGACGG ACCCCCCGAG GGTGTGTACA TGGTACAGCC CAAGGGCTGT 
AGTGACGAGG AGGACAATGC GCTTGATGAG CCCGAACCCA TGTTCGACTC TCGGGACGGC 
AGTTACCACG ACGACAAGGA CTCGGAAGAC GGGCCTCAAA AGGACGTCTA CATGCCGTTG 
ATAAGTGAGC CCCCGAGTCG CCAAACATCT TTCTCCTCTC CAGGAGAGGC GTCCACTCTG 
CGAGACTACG CAGCCAACAC AATGAACGAG TTCCTGGGCA TGTTCGGCTA CGACGACCAG 
CAGGTGAGGG ACGAGCTGGC CAAGAAGATC AGCTTCGAGA AGCTCAAAGC CGCTACCTCA 
GGCTCCGACC CATCCTCCCT GAGCAGCGAG GAGGCCACGC GGCGAGCCCG CTTTTCCAAG 
TATGAGGAGT ACATCCGCAA GCTGAAGGCT GGCGAGACCC TGCCCTGGCC CATCCACACC 
AACCCCAAAC AAGAGGACCT CAACTCCAAG CTTGCCCAGG AGAAGAATGC CCTGCTCCAG 
GCCCAGATGG CCGCCTCTGG GGGGTGCCAT TCCGGGGCTG AGGGACAGAT TTATCACTCC 
AGCCTGGACC ACAAGCAGGG GCAAGGGCAG GGGCACAACC TTGGGCACAA TCCCCCACCA 
ACCAACCCCA CCTCCCACCT CCAGAACATG GCCTCCCGAG CCTCTAAGTA TGACTTCTTC 
ATCCAGAAGC TGAAGATGGG TGAAAGCCTG CGGAGCCAGC AGAATGGCAA TGCTTACAAG 
AAGCCATCCA AGTACGACCT GGAGAATGTT CGATACCTGC ACCTCTTCAA GCCCGGCGAC 
GGGAACACTG ACATGGGGGG CGCCATCGCC TATAAAACGG GGAAGGTGGG CCGCCCGTCC 
AAGTATGATA TCAAGAACAT CCAGAAGCTG ATGCCGGGGA GGCCCGAGTC CTCGCTGATG 
CCCAGCGTTC TGCCTGCCAC ACCTGGGAAC CCGGGGACAC CTGTGGCTGC CACCTCGGCC 
TCCTCCGCTG GGGCCACGGG CCCCCCAGGA CTCATCATGG ATCAGACAGG CCACTTGGGC 
TTCAACACCT CCGACTACAT GAAGTCCAGC TTCTCCAAGA CCGACTCCAT CACCACGGGC 
ACTGTGTCTT CTGTCAAGAA CGGCTTACCA CCTGAGAAAC CTGTCACAGA GGACGTCAAC 
CTGTACCAGA AATATATCGC CAGAAGCCGT CAAACCTTCC CGAAGCCCCT ACCACCACCA 
CCACCACCAC CATACCCTGA GCCCCCCACC ACACCACCAA CTTTTAAAAG GTTCTCGGGG 
AGCCAGCACT GCGGACATGT GCATTGTGCC TACCAATACA GGGAGCATTA CCACTGCATG 
GACACGGAGT GCAACTACCA GAGGTTCACC AGCAAGCAAG ACGTGATCAG GCACTACAAT 
ATGCACAAGA AGAGGGACAA CTCCCTACAG CATGGCTTCA TGCGCTTCAG CCCACTGGAC 
GACTGTAGCG TCTACTACCA TGGCTGCCAC CTCAACGGGA AGAGCACCCA CTACCACTGC 
ATGCAGGTGG GCTGCAACAA GGTATACACC AGCACCTCCG ATGTCATGAC TCACGAGAAC 
TTCCATAAGA AGAACGCCCA GCTGATCAAC GATGGCTTTC AGAGGTTCCG CGCCACTGAG 
GATTGTGGGA CGGTGGAGTG CCAATTCTAC GGGCAGAAGA CCACCCACTT CCACTGCAGG 
CGCCCGGGGT GCACCTTCAC CTTCAAGAAC AAGTGCGACA TTGAGAAGCA CAAGAGCTAC 
CACATCAAGG ATGACGCATA TGCCAAGGAT GGCTTCAAGA AGTTCTACAA GTATGAGGAG 
TGCAAATACG AGGGCTGTGT CTACAGCAAG GCCACCAACC ACTTCCACTG CATCCGCTCA 
AGCTGCGGCT TCACCTTCAC CTCCACCAGC CAGATGACCT CCCACAAGCG CAAGCACGAG 
CGCCGCCACA TCCGCTCCTC GGGCGTGATG GCCCTCTCCT CTGGCGGCTC CTTCCTGATG 
CCCAAGGAGG AACCCGGAGA CGAGTCGAGC AACGACGACC TCATGGACTT CTCGGCCATC 
AGCAGCAAGA ACTCCAGCCT GAGCGCCTCG CCCACCACCC AGCAGTCGTC CAACGGTCCC 
GGCACCCACT TGCTTGCCAC ACCCACCACG GTGATGTCGT CCTCCGGCGG TGGCCACAGC 
GGCCTCAAGC CCACCTCCTC GCTGCCGCAC TCGGCACGCA TGTCAAGCCT GCTATCCCAG 
GCGCTGCCCA GCAACATGCC TGTGGCACTG GCGCTGTCCA ACAGTGCCCT CGCCAGCGTC 
AACAATCCCT TCTTCCCGCT CATGCCCCGG CTGCCCATGC AGCTGCCCCA TTCGGCCGCC 
AGCCTGATCT CGGCCGTGTC GTCTGGCGCC CACTCCATGC CCACCGACTC ACTGTCCCAG 
GGCGGCTGCT CCTCGGCGGG CAGCGATGGC GCCATGGCAT CCACGCCGAC CTCCTACGCC 
TCAGCCTCTT CCATCATGGA GAAGATCTCT GCTAGCAAGG GCCTCATATC CCCGATGATG 
GCCAGGCTGG CAGCAGCTGC CCTCAAGCCC TCTGGCAACC ACCAAGACAC AGGGAATGGG 
CTGGCTGGTT CTTCAGCGAG TCTGTTCAAT CTGGTTCAAG TGAAGCAGGA GCCAGTCGAC 
CATACCTCCG GGGCCTCCCA GGACTCCATC CAAGAGCACA GCCTGGACCT GAGCAAAAAA 
GACCACAGTA ATGAATCAAA CGGACACCCT GTACCGGGAA ATACATCTCT TTTATCCTCG 
CTTATGAATA AGATGAATGC CGGCTTCTTC AGTGCCCTGA ACCTGAAGAC GGAGATGGAG 
ATCGGCCAAG GGGCCGACAC CTCAGAGGCA GCACAGTACC TGAGCCGCAT CATGAAGAGG 
CCCATGCCAG ACAAACCAAG CGAGCTCTGG AGGACATATC TGCGCAGGTT TGACACGGAG 
GACTTCTGCG AGGCCCAGTG TGACTTCCTG CAGAAGGTCC ACTTCCATTG TCTAGTGGAG 
GACTGCGGGG CGCTCTTCAG CACGATGGAC GGAGCCATTA AACACGCCAA CTTCCACCTA 
CGAGCCACGC TGAAGGTGAA ATCAGAGCCT CACTTCAACA AAGTCAAGGA CTGCGGCGAC 
GGAGCATCCC ACCAGTCCAC CGCCCCTGTC TCCATGGCTA ACAACCCCGC CATGGACGTG 
GCCAACATGT CATCCTCGTC AGGCGGCGGC TATGGCTCCT CCCCCTCACT GCTGGCATGG 
AAGCAGCTGA CGGGCAGCAT CCCACAGCTC AATGCCTCCA TGCCTAACCT GCCTGCCAAT 
TCGCCGCTGG CCACCACCTC TCTGGAGAAC GCCAAGCCAC AGGTCAAACC GGGCTTCCTG 
CAGTTCCAGG ACAATTCATA CAGTGACCCT TGCCTGGCCA CTGACTGTAA GTACTCCAAC 
AAGGTCCACT TCCACTGTCT CTTCGGGAAC TGCAAGTACG TCTGCAAGAC CTCGGGGAAG 
GCCGAATCCC ACTGCTTGGA CCACATCAAT CCTAACAACC ACCTGGTCAA CGTTCGAGAC 
CAGTTCTCCT ACTTCTCCCT CCAGTGTCTA TGTCCCAACC AGCACTGTGA GTTCAGAATG 
AGGGGCCATT ACCACTGTCT GCGATCCGGC TGCTACTTTG TCACCAACAT CACCACCAAG 
CTTCCCTGGC ATGTCAAGAA GCATGAGAAG GCAGAGAGAC GCGCCGCCAA TGGCTTCAAA 
TACTTCACCA AGAGAGAGGA GTGTGGCAGG CTAGGCTGCA AGTACAACCA GGTGAACAGT 
CACTTCCACT GCATCCGCGA GAGCTGCCAG TTCTCCTTCC TGCTCAAGCA CCAGATGACC 
TCGCACTCCC GCAAACACAT GAGGAGAATG CTGGGAAAGA ACTTTGACAG GGTTCCATCC 
CAGGTGGTGT CCCATGGCCA GAGGGCCGAT GACATGCAGC ACTTGTCGGG CATGGGCTCA 
GGTCTCATGG GCAGCCACCC GGGGATGACC CATGGGTTCT CCAGCATGAT GGATGAGACG 
GATGACTACA TGGACTACAT GGGCGGGGGC AGCCCCCTGT GCCTTTCCTC TGAGTCCTCC 
AATCAGGACC GCAGTTGCAG CAGCACCCCC GTGGGCAATG AAAGCTCCCC AGCAGGACCA 
GGCTGCCCGG TCACTACTGC TCCCTCTACC CCCGCTGACA CTAGCGCTCC CCAAAACGCA 
CCTCCTCCAC CACCTCCTCC TCAGCCTGGA CTTCAGTCTC AGGCTCCATC TTTCCCTCCT 
GCCCTCCTCC GACCTCCTCT TCCCTCGCTC CCATATCTCG TCTCTCCATC CTGTCTGTCA 
TACTCTCTGC TCAGCGCCTC TCTCGGAGCC ACTCGGAGTG TTGTCATGCC AACCAACACA 
CCAGCTTTCA GCCCCATCAT TGCCACTCCG TCTCCGGTTA AAAATGACGT CCCTATAGTG 
CAGGATGCTG CAGGCAACAC CATCTCCATC CCTACGGCCA CCGGCGCCAA GAAACGCTTC 
TGGATCATCG AGGACATGTC CCCCTTCGGC AAGCGGCGTA AGACGGCTTC TGCTCGCAAA 
ATGCTGGACG AGGGCATGAT GCTGGAGGGC TTCCGCCGTT ATGATCTGTA CGAGGACTGC 
AAGGACTCCG GCTGCCAGTT CTCTCTGAAG GTGACCCACT ACCACTGCAC CCGCGAGAAC 
TGCGGCTACA AGTTCTGCGG CCGCACGCAC ATGTACAAGC ATGCGCAGCA CCACGACCGC 
GTGGACAACC TGGTCCTGGA CGACTTCAAG CGTTTCAAGT CATCGCTCAG CTGCAACTTC 
TCCGACTGCC AGTTCTCCGG CAACAGCACC CACTTCCACT GCCTGCGCTG CAGCTTCCGT 
TGCACGGACA GCACCAAGGT GACTGCCCAC CGCAAACACC ATGGCAAGCA AGATGTGATC 
AGCGCCGCTG GCTTCTGCCA GTTCAGCTCC AGCGCTGACT GCGAGGTTGT CGACTGCAAA 
TACAAGCTCA AGTGCTCACA CTTTCACTGC ACCTACCCCG AGTGCAAGCA CACTGTAGTC 
GGTATGTCCC AGATGGACTC CCACAAGCGC AAGCATGAGA AGCAGGAGCG CGGCGAGCTG 
CCGTCGGTGT CTCCCCGTCA GGAGGGGGCG CACCACATGA CGGGAGGAGT GTCCGCAATG 
CCCCACGCCT CCATGGGCCT TCTTCCAGCC TCGCCCGGTG GCCTCCCAGG ACTCTCCCAC 
CACCACCATC ACCACCATAA CAACAACAAC CATGCCCCCT CCATGCTCTA CCCCCCAGCC 
AGCGTGGCAT CTGACTACAA CCACCCCTAT CCAACCTCCT TGGTCAGTCT GGACAGCTCC 
CTCAACCTGG GCACCGACAC CAGCAGCTCC CTGTTCTTCC TGAAGAACGC CGCTGGCCTG 
GGCCTCAATG ACTCGTTGGA CCTGAGCAAA AAGATGCAGC ATCACCATGA CCCGAGCCTG 
ACCATGGCTG CCGGGCCCAA CCCCATGCCA CCGCTGGGCT TGCCCGCTCC TCAGGATGAC 
ACCACGGGAA CATCGGGCGA CCCCGAAGAT GACCAGTCAC CCGAGGAAGA GGCCAATGCA 
GAGGAAGATG AGGAGGATGA GGATGAGGCG GAGGAGGATC TGAACACTGA TTCGTACGAA 
GATTCGATGC CACAGCCCGA CGGCGACAAC AAGGACAATG ACGAGAGTTT TGACGCTTCC 
TTAAACCACA CTGACACTTC CCAACTGGAA AAGCAAGAGG CCGACCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

casz1 ( XM_021616106.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021471781.1 Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X2, mRNA.

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CloneID	OOf07775
Clone ID Related Accession (Same CDS sequence)	XM_021616106.1
Accession Version	XM_021616106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	zinc finger protein castor homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTA CATGTCTGAG AGGATTTAAC TTCGACTTCC CTGAATATGC TGAAAGGACT 
TTATGCACTG AAAAGACCTC AGGAAAATCC AACATGGCCG GCAAAAGGAA AGGTGGCCTA 
AAACTCAACG CAATCTGTGC CAAGCTGAGC CGTCAGGTGG TGTACGACGG CAGCTCCCAG 
CATGCTGAGG GAGACCACAG TGTGGCGGAG AACAGCGAGC GCGGGAGCTC CCATTATGAC 
GACAGCGGCG AGCGATTCGA GACCAACTTC CCCGAGGGCC TGAGTCTCGG CCAGAGCCTC 
GAAGAGGACC AGAAACGGCG CAAGGCCATT GAGAAGTGGG TCAACGGGGA GTATGGGGAT 
GAACCACCAC CCTCTGCCCT GGACTGTGGG GAGGAACAGC AGCAACAGCA GGGACTCAAG 
CCCAACAATG GCGAGGACGG ACCCCCCGAG GGTGTGTACA TGGTACAGCC CAAGGGCTGT 
AGTGACGAGG AGGACAATGC GCTTGATGAG CCCGAACCCA TGTTCGACTC TCGGGACGGC 
AGTTACCACG ACGACAAGGA CTCGGAAGAC GGGCCTCAAA AGGACGTCTA CATGCCGTTG 
ATAAGTGAGC CCCCGAGTCG CCAAACATCT TTCTCCTCTC CAGGAGAGGC GTCCACTCTG 
CGAGACTACG CAGCCAACAC AATGAACGAG TTCCTGGGCA TGTTCGGCTA CGACGACCAG 
CAGGTGAGGG ACGAGCTGGC CAAGAAGATC AGCTTCGAGA AGCTCAAAGC CGCTACCTCA 
GGCTCCGACC CATCCTCCCT GAGCAGCGAG GAGGCCACGC GGCGAGCCCG CTTTTCCAAG 
TATGAGGAGT ACATCCGCAA GCTGAAGGCT GGCGAGACCC TGCCCTGGCC CATCCACACC 
AACCCCAAAC AAGAGGACCT CAACTCCAAG CTTGCCCAGG AGAAGAATGC CCTGCTCCAG 
GCCCAGATGG CCGCCTCTGG GGGGTGCCAT TCCGGGGCTG AGGGACAGAT TTATCACTCC 
AGCCTGGACC ACAAGCAGGG GCAAGGGCAG GGGCACAACC TTGGGCACAA TCCCCCACCA 
ACCAACCCCA CCTCCCACCT CCAGAACATG GCCTCCCGAG CCTCTAAGTA TGACTTCTTC 
ATCCAGAAGC TGAAGATGGG TGAAAGCCTG CGGAGCCAGC AGAATGGCAA TGCTTACAAG 
AAGCCATCCA AGTACGACCT GGAGAATGTT CGATACCTGC ACCTCTTCAA GCCCGGCGAC 
GGGAACACTG ACATGGGGGG CGCCATCGCC TATAAAACGG GGAAGGTGGG CCGCCCGTCC 
AAGTATGATA TCAAGAACAT CCAGAAGCTG ATGCCGGGGA GGCCCGAGTC CTCGCTGATG 
CCCAGCGTTC TGCCTGCCAC ACCTGGGAAC CCGGGGACAC CTGTGGCTGC CACCTCGGCC 
TCCTCCGCTG GGGCCACGGG CCCCCCAGGA CTCATCATGG ATCAGACAGG CCACTTGGGC 
TTCAACACCT CCGACTACAT GAAGTCCAGC TTCTCCAAGA CCGACTCCAT CACCACGGGC 
ACTGTGTCTT CTGTCAAGAA CGGCTTACCA CCTGAGAAAC CTGTCACAGA GGACGTCAAC 
CTGTACCAGA AATATATCGC CAGAAGCCGT CAAACCTTCC CGAAGCCCCT ACCACCACCA 
CCACCACCAC CATACCCTGA GCCCCCCACC ACACCACCAA CTTTTAAAAG GTTCTCGGGG 
AGCCAGCACT GCGGACATGT GCATTGTGCC TACCAATACA GGGAGCATTA CCACTGCATG 
GACACGGAGT GCAACTACCA GAGGTTCACC AGCAAGCAAG ACGTGATCAG GCACTACAAT 
ATGCACAAGA AGAGGGACAA CTCCCTACAG CATGGCTTCA TGCGCTTCAG CCCACTGGAC 
GACTGTAGCG TCTACTACCA TGGCTGCCAC CTCAACGGGA AGAGCACCCA CTACCACTGC 
ATGCAGGTGG GCTGCAACAA GGTATACACC AGCACCTCCG ATGTCATGAC TCACGAGAAC 
TTCCATAAGA AGAACGCCCA GCTGATCAAC GATGGCTTTC AGAGGTTCCG CGCCACTGAG 
GATTGTGGGA CGGTGGAGTG CCAATTCTAC GGGCAGAAGA CCACCCACTT CCACTGCAGG 
CGCCCGGGGT GCACCTTCAC CTTCAAGAAC AAGTGCGACA TTGAGAAGCA CAAGAGCTAC 
CACATCAAGG ATGACGCATA TGCCAAGGAT GGCTTCAAGA AGTTCTACAA GTATGAGGAG 
TGCAAATACG AGGGCTGTGT CTACAGCAAG GCCACCAACC ACTTCCACTG CATCCGCTCA 
AGCTGCGGCT TCACCTTCAC CTCCACCAGC CAGATGACCT CCCACAAGCG CAAGCACGAG 
CGCCGCCACA TCCGCTCCTC GGGCGTGATG GCCCTCTCCT CTGGCGGCTC CTTCCTGATG 
CCCAAGGAGG AACCCGGAGA CGAGTCGAGC AACGACGACC TCATGGACTT CTCGGCCATC 
AGCAGCAAGA ACTCCAGCCT GAGCGCCTCG CCCACCACCC AGCAGTCGTC CAACGGTCCC 
GGCACCCACT TGCTTGCCAC ACCCACCACG GTGATGTCGT CCTCCGGCGG TGGCCACAGC 
GGCCTCAAGC CCACCTCCTC GCTGCCGCAC TCGGCACGCA TGTCAAGCCT GCTATCCCAG 
GCGCTGCCCA GCAACATGCC TGTGGCACTG GCGCTGTCCA ACAGTGCCCT CGCCAGCGTC 
AACAATCCCT TCTTCCCGCT CATGCCCCGG CTGCCCATGC AGCTGCCCCA TTCGGCCGCC 
AGCCTGATCT CGGCCGTGTC GTCTGGCGCC CACTCCATGC CCACCGACTC ACTGTCCCAG 
GGCGGCTGCT CCTCGGCGGG CAGCGATGGC GCCATGGCAT CCACGCCGAC CTCCTACGCC 
TCAGCCTCTT CCATCATGGA GAAGATCTCT GCTAGCAAGG GCCTCATATC CCCGATGATG 
GCCAGGCTGG CAGCAGCTGC CCTCAAGCCC TCTGGCAACC ACCAAGACAC AGGGAATGGG 
CTGGCTGGTT CTTCAGCGAG TCTGTTCAAT CTGGTTCAAG TGAAGCAGGA GCCAGTCGAC 
CATACCTCCG GGGCCTCCCA GGACTCCATC CAAGAGCACA GCCTGGACCT GAGCAAAAAA 
GACCACAGTA ATGAATCAAA CGGACACCCT GTACCGGGAA ATACATCTCT TTTATCCTCG 
CTTATGAATA AGATGAATGC CGGCTTCTTC AGTGCCCTGA ACCTGAAGAC GGAGATGGAG 
ATCGGCCAAG GGGCCGACAC CTCAGAGGCA GCACAGTACC TGAGCCGCAT CATGAAGAGG 
CCCATGCCAG ACAAACCAAG CGAGCTCTGG AGGACATATC TGCGCAGGTT TGACACGGAG 
GACTTCTGCG AGGCCCAGTG TGACTTCCTG CAGAAGGTCC ACTTCCATTG TCTAGTGGAG 
GACTGCGGGG CGCTCTTCAG CACGATGGAC GGAGCCATTA AACACGCCAA CTTCCACCTA 
CGAGCCACGC TGAAGGTGAA ATCAGAGCCT CACTTCAACA AAGTCAAGGA CTGCGGCGAC 
GGAGCATCCC ACCAGTCCAC CGCCCCTGTC TCCATGGCTA ACAACCCCGC CATGGACGTG 
GCCAACATGT CATCCTCGTC AGGCGGCGGC TATGGCTCCT CCCCCTCACT GCTGGCATGG 
AAGCAGCTGA CGGGCAGCAT CCCACAGCTC AATGCCTCCA TGCCTAACCT GCCTGCCAAT 
TCGCCGCTGG CCACCACCTC TCTGGAGAAC GCCAAGCCAC AGGTCAAACC GGGCTTCCTG 
CAGTTCCAGG ACAATGACCC TTGCCTGGCC ACTGACTGTA AGTACTCCAA CAAGGTCCAC 
TTCCACTGTC TCTTCGGGAA CTGCAAGTAC GTCTGCAAGA CCTCGGGGAA GGCCGAATCC 
CACTGCTTGG ACCACATCAA TCCTAACAAC CACCTGGTCA ACGTTCGAGA CCAGTTCTCC 
TACTTCTCCC TCCAGTGTCT ATGTCCCAAC CAGCACTGTG AGTTCAGAAT GAGGGGCCAT 
TACCACTGTC TGCGATCCGG CTGCTACTTT GTCACCAACA TCACCACCAA GCTTCCCTGG 
CATGTCAAGA AGCATGAGAA GGCAGAGAGA CGCGCCGCCA ATGGCTTCAA ATACTTCACC 
AAGAGAGAGG AGTGTGGCAG GCTAGGCTGC AAGTACAACC AGGTGAACAG TCACTTCCAC 
TGCATCCGCG AGAGCTGCCA GTTCTCCTTC CTGCTCAAGC ACCAGATGAC CTCGCACTCC 
CGCAAACACA TGAGGAGAAT GCTGGGAAAG AACTTTGACA GGGTTCCATC CCAGGTGGTG 
TCCCATGGCC AGAGGGCCGA TGACATGCAG CACTTGTCGG GCATGGGCTC AGGTCTCATG 
GGCAGCCACC CGGGGATGAC CCATGGGTTC TCCAGCATGA TGGATGAGAC GGATGACTAC 
ATGGACTACA TGGGCGGGGG CAGCCCCCTG TGCCTTTCCT CTGAGTCCTC CAATCAGGAC 
CGCAGTTGCA GCAGCACCCC CGTGGGCAAT GAAAGCTCCC CAGCAGGACC AGGCTGCCCG 
GTCACTACTG CTCCCTCTAC CCCCGCTGAC ACTAGCGCTC CCCAAAACGC ACCTCCTCCA 
CCACCTCCTC CTCAGCCTGG ACTTCAGTCT CAGGCTCCAT CTTTCCCTCC TGCCCTCCTC 
CGACCTCCTC TTCCCTCGCT CCCATATCTC GTCTCTCCAT CCTGTCTGTC ATACTCTCTG 
CTCAGCGCCT CTCTCGGAGC CACTCGGAGT GTTGTCATGC CAACCAACAC ACCAGCTTTC 
AGCCCCATCA TTGCCACTCC GTCTCCGGTT AAAAATGACG TCCCTATAGT GCAGGATGCT 
GCAGGCAACA CCATCTCCAT CCCTACGGCC ACCGGCGCCA AGAAACGCTT CTGGATCATC 
GAGGACATGT CCCCCTTCGG CAAGCGGCGT AAGACGGCTT CTGCTCGCAA AATGCTGGAC 
GAGGGCATGA TGCTGGAGGG CTTCCGCCGT TATGATCTGT ACGAGGACTG CAAGGACTCC 
GGCTGCCAGT TCTCTCTGAA GGTGACCCAC TACCACTGCA CCCGCGAGAA CTGCGGCTAC 
AAGTTCTGCG GCCGCACGCA CATGTACAAG CATGCGCAGC ACCACGACCG CGTGGACAAC 
CTGGTCCTGG ACGACTTCAA GCGTTTCAAG TCATCGCTCA GCTGCAACTT CTCCGACTGC 
CAGTTCTCCG GCAACAGCAC CCACTTCCAC TGCCTGCGCT GCAGCTTCCG TTGCACGGAC 
AGCACCAAGG TGACTGCCCA CCGCAAACAC CATGGCAAGC AAGATGTGAT CAGCGCCGCT 
GGCTTCTGCC AGTTCAGCTC CAGCGCTGAC TGCGAGGTTG TCGACTGCAA ATACAAGCTC 
AAGTGCTCAC ACTTTCACTG CACCTACCCC GAGTGCAAGC ACACTGTAGT CGGTATGTCC 
CAGATGGACT CCCACAAGCG CAAGCATGAG AAGCAGGAGC GCGGCGAGCT GCCGTCGGTG 
TCTCCCCGTC AGGAGGGGGC GCACCACATG ACGGGAGGAG TGTCCGCAAT GCCCCACGCC 
TCCATGGGCC TTCTTCCAGC CTCGCCCGGT GGCCTCCCAG GACTCTCCCA CCACCACCAT 
CACCACCATA ACAACAACAA CCATGCCCCC TCCATGCTCT ACCCCCCAGC CAGCGTGGCA 
TCTGACTACA ACCACCCCTA TCCAACCTCC TTGGTCAGTC TGGACAGCTC CCTCAACCTG 
GGCACCGACA CCAGCAGCTC CCTGTTCTTC CTGAAGAACG CCGCTGGCCT GGGCCTCAAT 
GACTCGTTGG ACCTGAGCAA AAAGATGCAG CATCACCATG ACCCGAGCCT GACCATGGCT 
GCCGGGCCCA ACCCCATGCC ACCGCTGGGC TTGCCCGCTC CTCAGGATGA CACCACGGGA 
ACATCGGGCG ACCCCGAAGA TGACCAGTCA CCCGAGGAAG AGGCCAATGC AGAGGAAGAT 
GAGGAGGATG AGGATGAGGC GGAGGAGGAT CTGAACACTG ATTCGTACGA AGATTCGATG 
CCACAGCCCG ACGGCGACAA CAAGGACAAT GACGAGAGTT TTGACGCTTC CTTAAACCAC 
ACTGACACTT CCCAACTGGA AAAGCAAGAG GCCGACCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021471781.1
CDS	355..6576
Translation	MKVTCLRGFNFDFPEYAERTLCTEKTSGKSNMAGKRKGGLKLNAICAKLSRQVVYDGSSQHAEGDHSVAENSERGSSHYDDSGERFETNFPEGLSLGQSLEEDQKRRKAIEKWVNGEYGDEPPPSALDCGEEQQQQQGLKPNNGEDGPPEGVYMVQPKGCSDEEDNALDEPEPMFDSRDGSYHDDKDSEDGPQKDVYMPLISEPPSRQTSFSSPGEASTLRDYAANTMNEFLGMFGYDDQQVRDELAKKISFEKLKAATSGSDPSSLSSEEATRRARFSKYEEYIRKLKAGETLPWPIHTNPKQEDLNSKLAQEKNALLQAQMAASGGCHSGAEGQIYHSSLDHKQGQGQGHNLGHNPPPTNPTSHLQNMASRASKYDFFIQKLKMGESLRSQQNGNAYKKPSKYDLENVRYLHLFKPGDGNTDMGGAIAYKTGKVGRPSKYDIKNIQKLMPGRPESSLMPSVLPATPGNPGTPVAATSASSAGATGPPGLIMDQTGHLGFNTSDYMKSSFSKTDSITTGTVSSVKNGLPPEKPVTEDVNLYQKYIARSRQTFPKPLPPPPPPPYPEPPTTPPTFKRFSGSQHCGHVHCAYQYREHYHCMDTECNYQRFTSKQDVIRHYNMHKKRDNSLQHGFMRFSPLDDCSVYYHGCHLNGKSTHYHCMQVGCNKVYTSTSDVMTHENFHKKNAQLINDGFQRFRATEDCGTVECQFYGQKTTHFHCRRPGCTFTFKNKCDIEKHKSYHIKDDAYAKDGFKKFYKYEECKYEGCVYSKATNHFHCIRSSCGFTFTSTSQMTSHKRKHERRHIRSSGVMALSSGGSFLMPKEEPGDESSNDDLMDFSAISSKNSSLSASPTTQQSSNGPGTHLLATPTTVMSSSGGGHSGLKPTSSLPHSARMSSLLSQALPSNMPVALALSNSALASVNNPFFPLMPRLPMQLPHSAASLISAVSSGAHSMPTDSLSQGGCSSAGSDGAMASTPTSYASASSIMEKISASKGLISPMMARLAAAALKPSGNHQDTGNGLAGSSASLFNLVQVKQEPVDHTSGASQDSIQEHSLDLSKKDHSNESNGHPVPGNTSLLSSLMNKMNAGFFSALNLKTEMEIGQGADTSEAAQYLSRIMKRPMPDKPSELWRTYLRRFDTEDFCEAQCDFLQKVHFHCLVEDCGALFSTMDGAIKHANFHLRATLKVKSEPHFNKVKDCGDGASHQSTAPVSMANNPAMDVANMSSSSGGGYGSSPSLLAWKQLTGSIPQLNASMPNLPANSPLATTSLENAKPQVKPGFLQFQDNDPCLATDCKYSNKVHFHCLFGNCKYVCKTSGKAESHCLDHINPNNHLVNVRDQFSYFSLQCLCPNQHCEFRMRGHYHCLRSGCYFVTNITTKLPWHVKKHEKAERRAANGFKYFTKREECGRLGCKYNQVNSHFHCIRESCQFSFLLKHQMTSHSRKHMRRMLGKNFDRVPSQVVSHGQRADDMQHLSGMGSGLMGSHPGMTHGFSSMMDETDDYMDYMGGGSPLCLSSESSNQDRSCSSTPVGNESSPAGPGCPVTTAPSTPADTSAPQNAPPPPPPPQPGLQSQAPSFPPALLRPPLPSLPYLVSPSCLSYSLLSASLGATRSVVMPTNTPAFSPIIATPSPVKNDVPIVQDAAGNTISIPTATGAKKRFWIIEDMSPFGKRRKTASARKMLDEGMMLEGFRRYDLYEDCKDSGCQFSLKVTHYHCTRENCGYKFCGRTHMYKHAQHHDRVDNLVLDDFKRFKSSLSCNFSDCQFSGNSTHFHCLRCSFRCTDSTKVTAHRKHHGKQDVISAAGFCQFSSSADCEVVDCKYKLKCSHFHCTYPECKHTVVGMSQMDSHKRKHEKQERGELPSVSPRQEGAHHMTGGVSAMPHASMGLLPASPGGLPGLSHHHHHHHNNNNHAPSMLYPPASVASDYNHPYPTSLVSLDSSLNLGTDTSSSLFFLKNAAGLGLNDSLDLSKKMQHHHDPSLTMAAGPNPMPPLGLPAPQDDTTGTSGDPEDDQSPEEEANAEEDEEDEDEAEEDLNTDSYEDSMPQPDGDNKDNDESFDASLNHTDTSQLEKQEADP

Target ORF information:

RefSeq Version	XM_021616106.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021616106.1

ORF Insert Sequence:

ATGAAAGTTA CATGTCTGAG AGGATTTAAC TTCGACTTCC CTGAATATGC TGAAAGGACT 
TTATGCACTG AAAAGACCTC AGGAAAATCC AACATGGCCG GCAAAAGGAA AGGTGGCCTA 
AAACTCAACG CAATCTGTGC CAAGCTGAGC CGTCAGGTGG TGTACGACGG CAGCTCCCAG 
CATGCTGAGG GAGACCACAG TGTGGCGGAG AACAGCGAGC GCGGGAGCTC CCATTATGAC 
GACAGCGGCG AGCGATTCGA GACCAACTTC CCCGAGGGCC TGAGTCTCGG CCAGAGCCTC 
GAAGAGGACC AGAAACGGCG CAAGGCCATT GAGAAGTGGG TCAACGGGGA GTATGGGGAT 
GAACCACCAC CCTCTGCCCT GGACTGTGGG GAGGAACAGC AGCAACAGCA GGGACTCAAG 
CCCAACAATG GCGAGGACGG ACCCCCCGAG GGTGTGTACA TGGTACAGCC CAAGGGCTGT 
AGTGACGAGG AGGACAATGC GCTTGATGAG CCCGAACCCA TGTTCGACTC TCGGGACGGC 
AGTTACCACG ACGACAAGGA CTCGGAAGAC GGGCCTCAAA AGGACGTCTA CATGCCGTTG 
ATAAGTGAGC CCCCGAGTCG CCAAACATCT TTCTCCTCTC CAGGAGAGGC GTCCACTCTG 
CGAGACTACG CAGCCAACAC AATGAACGAG TTCCTGGGCA TGTTCGGCTA CGACGACCAG 
CAGGTGAGGG ACGAGCTGGC CAAGAAGATC AGCTTCGAGA AGCTCAAAGC CGCTACCTCA 
GGCTCCGACC CATCCTCCCT GAGCAGCGAG GAGGCCACGC GGCGAGCCCG CTTTTCCAAG 
TATGAGGAGT ACATCCGCAA GCTGAAGGCT GGCGAGACCC TGCCCTGGCC CATCCACACC 
AACCCCAAAC AAGAGGACCT CAACTCCAAG CTTGCCCAGG AGAAGAATGC CCTGCTCCAG 
GCCCAGATGG CCGCCTCTGG GGGGTGCCAT TCCGGGGCTG AGGGACAGAT TTATCACTCC 
AGCCTGGACC ACAAGCAGGG GCAAGGGCAG GGGCACAACC TTGGGCACAA TCCCCCACCA 
ACCAACCCCA CCTCCCACCT CCAGAACATG GCCTCCCGAG CCTCTAAGTA TGACTTCTTC 
ATCCAGAAGC TGAAGATGGG TGAAAGCCTG CGGAGCCAGC AGAATGGCAA TGCTTACAAG 
AAGCCATCCA AGTACGACCT GGAGAATGTT CGATACCTGC ACCTCTTCAA GCCCGGCGAC 
GGGAACACTG ACATGGGGGG CGCCATCGCC TATAAAACGG GGAAGGTGGG CCGCCCGTCC 
AAGTATGATA TCAAGAACAT CCAGAAGCTG ATGCCGGGGA GGCCCGAGTC CTCGCTGATG 
CCCAGCGTTC TGCCTGCCAC ACCTGGGAAC CCGGGGACAC CTGTGGCTGC CACCTCGGCC 
TCCTCCGCTG GGGCCACGGG CCCCCCAGGA CTCATCATGG ATCAGACAGG CCACTTGGGC 
TTCAACACCT CCGACTACAT GAAGTCCAGC TTCTCCAAGA CCGACTCCAT CACCACGGGC 
ACTGTGTCTT CTGTCAAGAA CGGCTTACCA CCTGAGAAAC CTGTCACAGA GGACGTCAAC 
CTGTACCAGA AATATATCGC CAGAAGCCGT CAAACCTTCC CGAAGCCCCT ACCACCACCA 
CCACCACCAC CATACCCTGA GCCCCCCACC ACACCACCAA CTTTTAAAAG GTTCTCGGGG 
AGCCAGCACT GCGGACATGT GCATTGTGCC TACCAATACA GGGAGCATTA CCACTGCATG 
GACACGGAGT GCAACTACCA GAGGTTCACC AGCAAGCAAG ACGTGATCAG GCACTACAAT 
ATGCACAAGA AGAGGGACAA CTCCCTACAG CATGGCTTCA TGCGCTTCAG CCCACTGGAC 
GACTGTAGCG TCTACTACCA TGGCTGCCAC CTCAACGGGA AGAGCACCCA CTACCACTGC 
ATGCAGGTGG GCTGCAACAA GGTATACACC AGCACCTCCG ATGTCATGAC TCACGAGAAC 
TTCCATAAGA AGAACGCCCA GCTGATCAAC GATGGCTTTC AGAGGTTCCG CGCCACTGAG 
GATTGTGGGA CGGTGGAGTG CCAATTCTAC GGGCAGAAGA CCACCCACTT CCACTGCAGG 
CGCCCGGGGT GCACCTTCAC CTTCAAGAAC AAGTGCGACA TTGAGAAGCA CAAGAGCTAC 
CACATCAAGG ATGACGCATA TGCCAAGGAT GGCTTCAAGA AGTTCTACAA GTATGAGGAG 
TGCAAATACG AGGGCTGTGT CTACAGCAAG GCCACCAACC ACTTCCACTG CATCCGCTCA 
AGCTGCGGCT TCACCTTCAC CTCCACCAGC CAGATGACCT CCCACAAGCG CAAGCACGAG 
CGCCGCCACA TCCGCTCCTC GGGCGTGATG GCCCTCTCCT CTGGCGGCTC CTTCCTGATG 
CCCAAGGAGG AACCCGGAGA CGAGTCGAGC AACGACGACC TCATGGACTT CTCGGCCATC 
AGCAGCAAGA ACTCCAGCCT GAGCGCCTCG CCCACCACCC AGCAGTCGTC CAACGGTCCC 
GGCACCCACT TGCTTGCCAC ACCCACCACG GTGATGTCGT CCTCCGGCGG TGGCCACAGC 
GGCCTCAAGC CCACCTCCTC GCTGCCGCAC TCGGCACGCA TGTCAAGCCT GCTATCCCAG 
GCGCTGCCCA GCAACATGCC TGTGGCACTG GCGCTGTCCA ACAGTGCCCT CGCCAGCGTC 
AACAATCCCT TCTTCCCGCT CATGCCCCGG CTGCCCATGC AGCTGCCCCA TTCGGCCGCC 
AGCCTGATCT CGGCCGTGTC GTCTGGCGCC CACTCCATGC CCACCGACTC ACTGTCCCAG 
GGCGGCTGCT CCTCGGCGGG CAGCGATGGC GCCATGGCAT CCACGCCGAC CTCCTACGCC 
TCAGCCTCTT CCATCATGGA GAAGATCTCT GCTAGCAAGG GCCTCATATC CCCGATGATG 
GCCAGGCTGG CAGCAGCTGC CCTCAAGCCC TCTGGCAACC ACCAAGACAC AGGGAATGGG 
CTGGCTGGTT CTTCAGCGAG TCTGTTCAAT CTGGTTCAAG TGAAGCAGGA GCCAGTCGAC 
CATACCTCCG GGGCCTCCCA GGACTCCATC CAAGAGCACA GCCTGGACCT GAGCAAAAAA 
GACCACAGTA ATGAATCAAA CGGACACCCT GTACCGGGAA ATACATCTCT TTTATCCTCG 
CTTATGAATA AGATGAATGC CGGCTTCTTC AGTGCCCTGA ACCTGAAGAC GGAGATGGAG 
ATCGGCCAAG GGGCCGACAC CTCAGAGGCA GCACAGTACC TGAGCCGCAT CATGAAGAGG 
CCCATGCCAG ACAAACCAAG CGAGCTCTGG AGGACATATC TGCGCAGGTT TGACACGGAG 
GACTTCTGCG AGGCCCAGTG TGACTTCCTG CAGAAGGTCC ACTTCCATTG TCTAGTGGAG 
GACTGCGGGG CGCTCTTCAG CACGATGGAC GGAGCCATTA AACACGCCAA CTTCCACCTA 
CGAGCCACGC TGAAGGTGAA ATCAGAGCCT CACTTCAACA AAGTCAAGGA CTGCGGCGAC 
GGAGCATCCC ACCAGTCCAC CGCCCCTGTC TCCATGGCTA ACAACCCCGC CATGGACGTG 
GCCAACATGT CATCCTCGTC AGGCGGCGGC TATGGCTCCT CCCCCTCACT GCTGGCATGG 
AAGCAGCTGA CGGGCAGCAT CCCACAGCTC AATGCCTCCA TGCCTAACCT GCCTGCCAAT 
TCGCCGCTGG CCACCACCTC TCTGGAGAAC GCCAAGCCAC AGGTCAAACC GGGCTTCCTG 
CAGTTCCAGG ACAATGACCC TTGCCTGGCC ACTGACTGTA AGTACTCCAA CAAGGTCCAC 
TTCCACTGTC TCTTCGGGAA CTGCAAGTAC GTCTGCAAGA CCTCGGGGAA GGCCGAATCC 
CACTGCTTGG ACCACATCAA TCCTAACAAC CACCTGGTCA ACGTTCGAGA CCAGTTCTCC 
TACTTCTCCC TCCAGTGTCT ATGTCCCAAC CAGCACTGTG AGTTCAGAAT GAGGGGCCAT 
TACCACTGTC TGCGATCCGG CTGCTACTTT GTCACCAACA TCACCACCAA GCTTCCCTGG 
CATGTCAAGA AGCATGAGAA GGCAGAGAGA CGCGCCGCCA ATGGCTTCAA ATACTTCACC 
AAGAGAGAGG AGTGTGGCAG GCTAGGCTGC AAGTACAACC AGGTGAACAG TCACTTCCAC 
TGCATCCGCG AGAGCTGCCA GTTCTCCTTC CTGCTCAAGC ACCAGATGAC CTCGCACTCC 
CGCAAACACA TGAGGAGAAT GCTGGGAAAG AACTTTGACA GGGTTCCATC CCAGGTGGTG 
TCCCATGGCC AGAGGGCCGA TGACATGCAG CACTTGTCGG GCATGGGCTC AGGTCTCATG 
GGCAGCCACC CGGGGATGAC CCATGGGTTC TCCAGCATGA TGGATGAGAC GGATGACTAC 
ATGGACTACA TGGGCGGGGG CAGCCCCCTG TGCCTTTCCT CTGAGTCCTC CAATCAGGAC 
CGCAGTTGCA GCAGCACCCC CGTGGGCAAT GAAAGCTCCC CAGCAGGACC AGGCTGCCCG 
GTCACTACTG CTCCCTCTAC CCCCGCTGAC ACTAGCGCTC CCCAAAACGC ACCTCCTCCA 
CCACCTCCTC CTCAGCCTGG ACTTCAGTCT CAGGCTCCAT CTTTCCCTCC TGCCCTCCTC 
CGACCTCCTC TTCCCTCGCT CCCATATCTC GTCTCTCCAT CCTGTCTGTC ATACTCTCTG 
CTCAGCGCCT CTCTCGGAGC CACTCGGAGT GTTGTCATGC CAACCAACAC ACCAGCTTTC 
AGCCCCATCA TTGCCACTCC GTCTCCGGTT AAAAATGACG TCCCTATAGT GCAGGATGCT 
GCAGGCAACA CCATCTCCAT CCCTACGGCC ACCGGCGCCA AGAAACGCTT CTGGATCATC 
GAGGACATGT CCCCCTTCGG CAAGCGGCGT AAGACGGCTT CTGCTCGCAA AATGCTGGAC 
GAGGGCATGA TGCTGGAGGG CTTCCGCCGT TATGATCTGT ACGAGGACTG CAAGGACTCC 
GGCTGCCAGT TCTCTCTGAA GGTGACCCAC TACCACTGCA CCCGCGAGAA CTGCGGCTAC 
AAGTTCTGCG GCCGCACGCA CATGTACAAG CATGCGCAGC ACCACGACCG CGTGGACAAC 
CTGGTCCTGG ACGACTTCAA GCGTTTCAAG TCATCGCTCA GCTGCAACTT CTCCGACTGC 
CAGTTCTCCG GCAACAGCAC CCACTTCCAC TGCCTGCGCT GCAGCTTCCG TTGCACGGAC 
AGCACCAAGG TGACTGCCCA CCGCAAACAC CATGGCAAGC AAGATGTGAT CAGCGCCGCT 
GGCTTCTGCC AGTTCAGCTC CAGCGCTGAC TGCGAGGTTG TCGACTGCAA ATACAAGCTC 
AAGTGCTCAC ACTTTCACTG CACCTACCCC GAGTGCAAGC ACACTGTAGT CGGTATGTCC 
CAGATGGACT CCCACAAGCG CAAGCATGAG AAGCAGGAGC GCGGCGAGCT GCCGTCGGTG 
TCTCCCCGTC AGGAGGGGGC GCACCACATG ACGGGAGGAG TGTCCGCAAT GCCCCACGCC 
TCCATGGGCC TTCTTCCAGC CTCGCCCGGT GGCCTCCCAG GACTCTCCCA CCACCACCAT 
CACCACCATA ACAACAACAA CCATGCCCCC TCCATGCTCT ACCCCCCAGC CAGCGTGGCA 
TCTGACTACA ACCACCCCTA TCCAACCTCC TTGGTCAGTC TGGACAGCTC CCTCAACCTG 
GGCACCGACA CCAGCAGCTC CCTGTTCTTC CTGAAGAACG CCGCTGGCCT GGGCCTCAAT 
GACTCGTTGG ACCTGAGCAA AAAGATGCAG CATCACCATG ACCCGAGCCT GACCATGGCT 
GCCGGGCCCA ACCCCATGCC ACCGCTGGGC TTGCCCGCTC CTCAGGATGA CACCACGGGA 
ACATCGGGCG ACCCCGAAGA TGACCAGTCA CCCGAGGAAG AGGCCAATGC AGAGGAAGAT 
GAGGAGGATG AGGATGAGGC GGAGGAGGAT CTGAACACTG ATTCGTACGA AGATTCGATG 
CCACAGCCCG ACGGCGACAA CAAGGACAAT GACGAGAGTT TTGACGCTTC CTTAAACCAC 
ACTGACACTT CCCAACTGGA AAAGCAAGAG GCCGACCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

casz1 ( XM_021616107.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021471782.1 Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X3, mRNA.

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CloneID	OOf07776
Clone ID Related Accession (Same CDS sequence)	XM_021616107.1
Accession Version	XM_021616107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	zinc finger protein castor homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTA CATGTCTGAG AGGATTTAAC TTCGACTTCC CTGAATATGC TGAAAGGACT 
TTATGCACTG AAAAGACCTC AGGAAAATCC AACATGGCCG GCAAAAGGAA AGGTGGCCTA 
AAACTCAACG CAATCTGTGC CAAGCTGAGC CGTCAGGTGG TGTACGACGG CAGCTCCCAG 
CATGCTGAGG GAGACCACAG TGTGGCGGAG AACAGCGAGC GCGGGAGCTC CCATTATGAC 
GACAGCGGCG AGCGATTCGA GACCAACTTC CCCGAGGGCC TGAGTCTCGG CCAGAGCCTC 
GAAGAGGACC AGAAACGGCG CAAGGCCATT GAGAAGTGGG TCAACGGGGA GTATGGGGAT 
GAACCACCAC CCTCTGCCCT GGACTGTGGG GAGGAACAGC AGCAACAGCA GGGACTCAAG 
CCCAACAATG GCGAGGACGG ACCCCCCGAG GGTGTGTACA TGGTACAGCC CAAGGGCTGT 
AGTGACGAGG AGGACAATGC GCTTGATGAG CCCGAACCCA TGTTCGACTC TCGGGACGGC 
AGTTACCACG ACGACAAGGA CTCGGAAGAC GGGCCTCAAA AGGACGTCTA CATGCCGTTG 
ATAAGTGAGC CCCCGAGTCG CCAAACATCT TTCTCCTCTC CAGGAGAGGC GTCCACTCTG 
CGAGACTACG CAGCCAACAC AATGAACGAG TTCCTGGGCA TGTTCGGCTA CGACGACCAG 
CAGGTGAGGG ACGAGCTGGC CAAGAAGATC AGCTTCGAGA AGCTCAAAGC CGCTACCTCA 
GGCTCCGACC CATCCTCCCT GAGCAGCGAG GAGGCCACGC GGCGAGCCCG CTTTTCCAAG 
TATGAGGAGT ACATCCGCAA GCTGAAGGCT GGCGAGACCC TGCCCTGGCC CATCCACACC 
AACCCCAAAC AAGAGGACCT CAACTCCAAG CTTGCCCAGG AGAAGAATGC CCTGCTCCAG 
GCCCAGATGG CCGCCTCTGG GGGGTGCCAT TCCGGGGCTG AGGGACAGAT TTATCACTCC 
AGCCTGGACC ACAAGCAGGG GCAAGGGCAG GGGCACAACC TTGGGCACAA TCCCCCACCA 
ACCAACCCCA CCTCCCACCT CCAGAACATG GCCTCCCGAG CCTCTAAGTA TGACTTCTTC 
ATCCAGAAGC TGAAGATGGG TGAAAGCCTG CGGAGCCAGC AGAATGGCAA TGCTTACAAG 
AAGCCATCCA AGTACGACCT GGAGAATGTT CGATACCTGC ACCTCTTCAA GCCCGGCGAC 
GGGAACACTG ACATGGGGGG CGCCATCGCC TATAAAACGG GGAAGGTGGG CCGCCCGTCC 
AAGTATGATA TCAAGAACAT CCAGAAGCTG ATGCCGGGGA GGCCCGAGTC CTCGCTGATG 
CCCAGCGTTC TGCCTGCCAC ACCTGGGAAC CCGGGGACAC CTGTGGCTGC CACCTCGGCC 
TCCTCCGCTG GGGCCACGGG CCCCCCAGGA CTCATCATGG ATCAGACAGG CCACTTGGGC 
TTCAACACCT CCGACTACAT GAAGTCCAGC TTCTCCAAGA CCGACTCCAT CACCACGGGC 
ACTGTGTCTT CTGTCAAGAA CGGCTTACCA CCTGAGAAAC CTGTCACAGA GGACGTCAAC 
CTGTACCAGA AATATATCGC CAGGTTCTCG GGGAGCCAGC ACTGCGGACA TGTGCATTGT 
GCCTACCAAT ACAGGGAGCA TTACCACTGC ATGGACACGG AGTGCAACTA CCAGAGGTTC 
ACCAGCAAGC AAGACGTGAT CAGGCACTAC AATATGCACA AGAAGAGGGA CAACTCCCTA 
CAGCATGGCT TCATGCGCTT CAGCCCACTG GACGACTGTA GCGTCTACTA CCATGGCTGC 
CACCTCAACG GGAAGAGCAC CCACTACCAC TGCATGCAGG TGGGCTGCAA CAAGGTATAC 
ACCAGCACCT CCGATGTCAT GACTCACGAG AACTTCCATA AGAAGAACGC CCAGCTGATC 
AACGATGGCT TTCAGAGGTT CCGCGCCACT GAGGATTGTG GGACGGTGGA GTGCCAATTC 
TACGGGCAGA AGACCACCCA CTTCCACTGC AGGCGCCCGG GGTGCACCTT CACCTTCAAG 
AACAAGTGCG ACATTGAGAA GCACAAGAGC TACCACATCA AGGATGACGC ATATGCCAAG 
GATGGCTTCA AGAAGTTCTA CAAGTATGAG GAGTGCAAAT ACGAGGGCTG TGTCTACAGC 
AAGGCCACCA ACCACTTCCA CTGCATCCGC TCAAGCTGCG GCTTCACCTT CACCTCCACC 
AGCCAGATGA CCTCCCACAA GCGCAAGCAC GAGCGCCGCC ACATCCGCTC CTCGGGCGTG 
ATGGCCCTCT CCTCTGGCGG CTCCTTCCTG ATGCCCAAGG AGGAACCCGG AGACGAGTCG 
AGCAACGACG ACCTCATGGA CTTCTCGGCC ATCAGCAGCA AGAACTCCAG CCTGAGCGCC 
TCGCCCACCA CCCAGCAGTC GTCCAACGGT CCCGGCACCC ACTTGCTTGC CACACCCACC 
ACGGTGATGT CGTCCTCCGG CGGTGGCCAC AGCGGCCTCA AGCCCACCTC CTCGCTGCCG 
CACTCGGCAC GCATGTCAAG CCTGCTATCC CAGGCGCTGC CCAGCAACAT GCCTGTGGCA 
CTGGCGCTGT CCAACAGTGC CCTCGCCAGC GTCAACAATC CCTTCTTCCC GCTCATGCCC 
CGGCTGCCCA TGCAGCTGCC CCATTCGGCC GCCAGCCTGA TCTCGGCCGT GTCGTCTGGC 
GCCCACTCCA TGCCCACCGA CTCACTGTCC CAGGGCGGCT GCTCCTCGGC GGGCAGCGAT 
GGCGCCATGG CATCCACGCC GACCTCCTAC GCCTCAGCCT CTTCCATCAT GGAGAAGATC 
TCTGCTAGCA AGGGCCTCAT ATCCCCGATG ATGGCCAGGC TGGCAGCAGC TGCCCTCAAG 
CCCTCTGGCA ACCACCAAGA CACAGGGAAT GGGCTGGCTG GTTCTTCAGC GAGTCTGTTC 
AATCTGGTTC AAGTGAAGCA GGAGCCAGTC GACCATACCT CCGGGGCCTC CCAGGACTCC 
ATCCAAGAGC ACAGCCTGGA CCTGAGCAAA AAAGACCACA GTAATGAATC AAACGGACAC 
CCTGTACCGG GAAATACATC TCTTTTATCC TCGCTTATGA ATAAGATGAA TGCCGGCTTC 
TTCAGTGCCC TGAACCTGAA GACGGAGATG GAGATCGGCC AAGGGGCCGA CACCTCAGAG 
GCAGCACAGT ACCTGAGCCG CATCATGAAG AGGCCCATGC CAGACAAACC AAGCGAGCTC 
TGGAGGACAT ATCTGCGCAG GTTTGACACG GAGGACTTCT GCGAGGCCCA GTGTGACTTC 
CTGCAGAAGG TCCACTTCCA TTGTCTAGTG GAGGACTGCG GGGCGCTCTT CAGCACGATG 
GACGGAGCCA TTAAACACGC CAACTTCCAC CTACGAGCCA CGCTGAAGGT GAAATCAGAG 
CCTCACTTCA ACAAAGTCAA GGACTGCGGC GACGGAGCAT CCCACCAGTC CACCGCCCCT 
GTCTCCATGG CTAACAACCC CGCCATGGAC GTGGCCAACA TGTCATCCTC GTCAGGCGGC 
GGCTATGGCT CCTCCCCCTC ACTGCTGGCA TGGAAGCAGC TGACGGGCAG CATCCCACAG 
CTCAATGCCT CCATGCCTAA CCTGCCTGCC AATTCGCCGC TGGCCACCAC CTCTCTGGAG 
AACGCCAAGC CACAGGTCAA ACCGGGCTTC CTGCAGTTCC AGGACAATTC ATACAGTGAC 
CCTTGCCTGG CCACTGACTG TAAGTACTCC AACAAGGTCC ACTTCCACTG TCTCTTCGGG 
AACTGCAAGT ACGTCTGCAA GACCTCGGGG AAGGCCGAAT CCCACTGCTT GGACCACATC 
AATCCTAACA ACCACCTGGT CAACGTTCGA GACCAGTTCT CCTACTTCTC CCTCCAGTGT 
CTATGTCCCA ACCAGCACTG TGAGTTCAGA ATGAGGGGCC ATTACCACTG TCTGCGATCC 
GGCTGCTACT TTGTCACCAA CATCACCACC AAGCTTCCCT GGCATGTCAA GAAGCATGAG 
AAGGCAGAGA GACGCGCCGC CAATGGCTTC AAATACTTCA CCAAGAGAGA GGAGTGTGGC 
AGGCTAGGCT GCAAGTACAA CCAGGTGAAC AGTCACTTCC ACTGCATCCG CGAGAGCTGC 
CAGTTCTCCT TCCTGCTCAA GCACCAGATG ACCTCGCACT CCCGCAAACA CATGAGGAGA 
ATGCTGGGAA AGAACTTTGA CAGGGTTCCA TCCCAGGTGG TGTCCCATGG CCAGAGGGCC 
GATGACATGC AGCACTTGTC GGGCATGGGC TCAGGTCTCA TGGGCAGCCA CCCGGGGATG 
ACCCATGGGT TCTCCAGCAT GATGGATGAG ACGGATGACT ACATGGACTA CATGGGCGGG 
GGCAGCCCCC TGTGCCTTTC CTCTGAGTCC TCCAATCAGG ACCGCAGTTG CAGCAGCACC 
CCCGTGGGCA ATGAAAGCTC CCCAGCAGGA CCAGGCTGCC CGGTCACTAC TGCTCCCTCT 
ACCCCCGCTG ACACTAGCGC TCCCCAAAAC GCACCTCCTC CACCACCTCC TCCTCAGCCT 
GGACTTCAGT CTCAGGCTCC ATCTTTCCCT CCTGCCCTCC TCCGACCTCC TCTTCCCTCG 
CTCCCATATC TCGTCTCTCC ATCCTGTCTG TCATACTCTC TGCTCAGCGC CTCTCTCGGA 
GCCACTCGGA GTGTTGTCAT GCCAACCAAC ACACCAGCTT TCAGCCCCAT CATTGCCACT 
CCGTCTCCGG TTAAAAATGA CGTCCCTATA GTGCAGGATG CTGCAGGCAA CACCATCTCC 
ATCCCTACGG CCACCGGCGC CAAGAAACGC TTCTGGATCA TCGAGGACAT GTCCCCCTTC 
GGCAAGCGGC GTAAGACGGC TTCTGCTCGC AAAATGCTGG ACGAGGGCAT GATGCTGGAG 
GGCTTCCGCC GTTATGATCT GTACGAGGAC TGCAAGGACT CCGGCTGCCA GTTCTCTCTG 
AAGGTGACCC ACTACCACTG CACCCGCGAG AACTGCGGCT ACAAGTTCTG CGGCCGCACG 
CACATGTACA AGCATGCGCA GCACCACGAC CGCGTGGACA ACCTGGTCCT GGACGACTTC 
AAGCGTTTCA AGTCATCGCT CAGCTGCAAC TTCTCCGACT GCCAGTTCTC CGGCAACAGC 
ACCCACTTCC ACTGCCTGCG CTGCAGCTTC CGTTGCACGG ACAGCACCAA GGTGACTGCC 
CACCGCAAAC ACCATGGCAA GCAAGATGTG ATCAGCGCCG CTGGCTTCTG CCAGTTCAGC 
TCCAGCGCTG ACTGCGAGGT TGTCGACTGC AAATACAAGC TCAAGTGCTC ACACTTTCAC 
TGCACCTACC CCGAGTGCAA GCACACTGTA GTCGGTATGT CCCAGATGGA CTCCCACAAG 
CGCAAGCATG AGAAGCAGGA GCGCGGCGAG CTGCCGTCGG TGTCTCCCCG TCAGGAGGGG 
GCGCACCACA TGACGGGAGG AGTGTCCGCA ATGCCCCACG CCTCCATGGG CCTTCTTCCA 
GCCTCGCCCG GTGGCCTCCC AGGACTCTCC CACCACCACC ATCACCACCA TAACAACAAC 
AACCATGCCC CCTCCATGCT CTACCCCCCA GCCAGCGTGG CATCTGACTA CAACCACCCC 
TATCCAACCT CCTTGGTCAG TCTGGACAGC TCCCTCAACC TGGGCACCGA CACCAGCAGC 
TCCCTGTTCT TCCTGAAGAA CGCCGCTGGC CTGGGCCTCA ATGACTCGTT GGACCTGAGC 
AAAAAGATGC AGCATCACCA TGACCCGAGC CTGACCATGG CTGCCGGGCC CAACCCCATG 
CCACCGCTGG GCTTGCCCGC TCCTCAGGAT GACACCACGG GAACATCGGG CGACCCCGAA 
GATGACCAGT CACCCGAGGA AGAGGCCAAT GCAGAGGAAG ATGAGGAGGA TGAGGATGAG 
GCGGAGGAGG ATCTGAACAC TGATTCGTAC GAAGATTCGA TGCCACAGCC CGACGGCGAC 
AACAAGGACA ATGACGAGAG TTTTGACGCT TCCTTAAACC ACACTGACAC TTCCCAACTG 
GAAAAGCAAG AGGCCGACCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021471782.1
CDS	355..6498
Translation	MKVTCLRGFNFDFPEYAERTLCTEKTSGKSNMAGKRKGGLKLNAICAKLSRQVVYDGSSQHAEGDHSVAENSERGSSHYDDSGERFETNFPEGLSLGQSLEEDQKRRKAIEKWVNGEYGDEPPPSALDCGEEQQQQQGLKPNNGEDGPPEGVYMVQPKGCSDEEDNALDEPEPMFDSRDGSYHDDKDSEDGPQKDVYMPLISEPPSRQTSFSSPGEASTLRDYAANTMNEFLGMFGYDDQQVRDELAKKISFEKLKAATSGSDPSSLSSEEATRRARFSKYEEYIRKLKAGETLPWPIHTNPKQEDLNSKLAQEKNALLQAQMAASGGCHSGAEGQIYHSSLDHKQGQGQGHNLGHNPPPTNPTSHLQNMASRASKYDFFIQKLKMGESLRSQQNGNAYKKPSKYDLENVRYLHLFKPGDGNTDMGGAIAYKTGKVGRPSKYDIKNIQKLMPGRPESSLMPSVLPATPGNPGTPVAATSASSAGATGPPGLIMDQTGHLGFNTSDYMKSSFSKTDSITTGTVSSVKNGLPPEKPVTEDVNLYQKYIARFSGSQHCGHVHCAYQYREHYHCMDTECNYQRFTSKQDVIRHYNMHKKRDNSLQHGFMRFSPLDDCSVYYHGCHLNGKSTHYHCMQVGCNKVYTSTSDVMTHENFHKKNAQLINDGFQRFRATEDCGTVECQFYGQKTTHFHCRRPGCTFTFKNKCDIEKHKSYHIKDDAYAKDGFKKFYKYEECKYEGCVYSKATNHFHCIRSSCGFTFTSTSQMTSHKRKHERRHIRSSGVMALSSGGSFLMPKEEPGDESSNDDLMDFSAISSKNSSLSASPTTQQSSNGPGTHLLATPTTVMSSSGGGHSGLKPTSSLPHSARMSSLLSQALPSNMPVALALSNSALASVNNPFFPLMPRLPMQLPHSAASLISAVSSGAHSMPTDSLSQGGCSSAGSDGAMASTPTSYASASSIMEKISASKGLISPMMARLAAAALKPSGNHQDTGNGLAGSSASLFNLVQVKQEPVDHTSGASQDSIQEHSLDLSKKDHSNESNGHPVPGNTSLLSSLMNKMNAGFFSALNLKTEMEIGQGADTSEAAQYLSRIMKRPMPDKPSELWRTYLRRFDTEDFCEAQCDFLQKVHFHCLVEDCGALFSTMDGAIKHANFHLRATLKVKSEPHFNKVKDCGDGASHQSTAPVSMANNPAMDVANMSSSSGGGYGSSPSLLAWKQLTGSIPQLNASMPNLPANSPLATTSLENAKPQVKPGFLQFQDNSYSDPCLATDCKYSNKVHFHCLFGNCKYVCKTSGKAESHCLDHINPNNHLVNVRDQFSYFSLQCLCPNQHCEFRMRGHYHCLRSGCYFVTNITTKLPWHVKKHEKAERRAANGFKYFTKREECGRLGCKYNQVNSHFHCIRESCQFSFLLKHQMTSHSRKHMRRMLGKNFDRVPSQVVSHGQRADDMQHLSGMGSGLMGSHPGMTHGFSSMMDETDDYMDYMGGGSPLCLSSESSNQDRSCSSTPVGNESSPAGPGCPVTTAPSTPADTSAPQNAPPPPPPPQPGLQSQAPSFPPALLRPPLPSLPYLVSPSCLSYSLLSASLGATRSVVMPTNTPAFSPIIATPSPVKNDVPIVQDAAGNTISIPTATGAKKRFWIIEDMSPFGKRRKTASARKMLDEGMMLEGFRRYDLYEDCKDSGCQFSLKVTHYHCTRENCGYKFCGRTHMYKHAQHHDRVDNLVLDDFKRFKSSLSCNFSDCQFSGNSTHFHCLRCSFRCTDSTKVTAHRKHHGKQDVISAAGFCQFSSSADCEVVDCKYKLKCSHFHCTYPECKHTVVGMSQMDSHKRKHEKQERGELPSVSPRQEGAHHMTGGVSAMPHASMGLLPASPGGLPGLSHHHHHHHNNNNHAPSMLYPPASVASDYNHPYPTSLVSLDSSLNLGTDTSSSLFFLKNAAGLGLNDSLDLSKKMQHHHDPSLTMAAGPNPMPPLGLPAPQDDTTGTSGDPEDDQSPEEEANAEEDEEDEDEAEEDLNTDSYEDSMPQPDGDNKDNDESFDASLNHTDTSQLEKQEADP

Target ORF information:

RefSeq Version	XM_021616107.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021616107.1

ORF Insert Sequence:

ATGAAAGTTA CATGTCTGAG AGGATTTAAC TTCGACTTCC CTGAATATGC TGAAAGGACT 
TTATGCACTG AAAAGACCTC AGGAAAATCC AACATGGCCG GCAAAAGGAA AGGTGGCCTA 
AAACTCAACG CAATCTGTGC CAAGCTGAGC CGTCAGGTGG TGTACGACGG CAGCTCCCAG 
CATGCTGAGG GAGACCACAG TGTGGCGGAG AACAGCGAGC GCGGGAGCTC CCATTATGAC 
GACAGCGGCG AGCGATTCGA GACCAACTTC CCCGAGGGCC TGAGTCTCGG CCAGAGCCTC 
GAAGAGGACC AGAAACGGCG CAAGGCCATT GAGAAGTGGG TCAACGGGGA GTATGGGGAT 
GAACCACCAC CCTCTGCCCT GGACTGTGGG GAGGAACAGC AGCAACAGCA GGGACTCAAG 
CCCAACAATG GCGAGGACGG ACCCCCCGAG GGTGTGTACA TGGTACAGCC CAAGGGCTGT 
AGTGACGAGG AGGACAATGC GCTTGATGAG CCCGAACCCA TGTTCGACTC TCGGGACGGC 
AGTTACCACG ACGACAAGGA CTCGGAAGAC GGGCCTCAAA AGGACGTCTA CATGCCGTTG 
ATAAGTGAGC CCCCGAGTCG CCAAACATCT TTCTCCTCTC CAGGAGAGGC GTCCACTCTG 
CGAGACTACG CAGCCAACAC AATGAACGAG TTCCTGGGCA TGTTCGGCTA CGACGACCAG 
CAGGTGAGGG ACGAGCTGGC CAAGAAGATC AGCTTCGAGA AGCTCAAAGC CGCTACCTCA 
GGCTCCGACC CATCCTCCCT GAGCAGCGAG GAGGCCACGC GGCGAGCCCG CTTTTCCAAG 
TATGAGGAGT ACATCCGCAA GCTGAAGGCT GGCGAGACCC TGCCCTGGCC CATCCACACC 
AACCCCAAAC AAGAGGACCT CAACTCCAAG CTTGCCCAGG AGAAGAATGC CCTGCTCCAG 
GCCCAGATGG CCGCCTCTGG GGGGTGCCAT TCCGGGGCTG AGGGACAGAT TTATCACTCC 
AGCCTGGACC ACAAGCAGGG GCAAGGGCAG GGGCACAACC TTGGGCACAA TCCCCCACCA 
ACCAACCCCA CCTCCCACCT CCAGAACATG GCCTCCCGAG CCTCTAAGTA TGACTTCTTC 
ATCCAGAAGC TGAAGATGGG TGAAAGCCTG CGGAGCCAGC AGAATGGCAA TGCTTACAAG 
AAGCCATCCA AGTACGACCT GGAGAATGTT CGATACCTGC ACCTCTTCAA GCCCGGCGAC 
GGGAACACTG ACATGGGGGG CGCCATCGCC TATAAAACGG GGAAGGTGGG CCGCCCGTCC 
AAGTATGATA TCAAGAACAT CCAGAAGCTG ATGCCGGGGA GGCCCGAGTC CTCGCTGATG 
CCCAGCGTTC TGCCTGCCAC ACCTGGGAAC CCGGGGACAC CTGTGGCTGC CACCTCGGCC 
TCCTCCGCTG GGGCCACGGG CCCCCCAGGA CTCATCATGG ATCAGACAGG CCACTTGGGC 
TTCAACACCT CCGACTACAT GAAGTCCAGC TTCTCCAAGA CCGACTCCAT CACCACGGGC 
ACTGTGTCTT CTGTCAAGAA CGGCTTACCA CCTGAGAAAC CTGTCACAGA GGACGTCAAC 
CTGTACCAGA AATATATCGC CAGGTTCTCG GGGAGCCAGC ACTGCGGACA TGTGCATTGT 
GCCTACCAAT ACAGGGAGCA TTACCACTGC ATGGACACGG AGTGCAACTA CCAGAGGTTC 
ACCAGCAAGC AAGACGTGAT CAGGCACTAC AATATGCACA AGAAGAGGGA CAACTCCCTA 
CAGCATGGCT TCATGCGCTT CAGCCCACTG GACGACTGTA GCGTCTACTA CCATGGCTGC 
CACCTCAACG GGAAGAGCAC CCACTACCAC TGCATGCAGG TGGGCTGCAA CAAGGTATAC 
ACCAGCACCT CCGATGTCAT GACTCACGAG AACTTCCATA AGAAGAACGC CCAGCTGATC 
AACGATGGCT TTCAGAGGTT CCGCGCCACT GAGGATTGTG GGACGGTGGA GTGCCAATTC 
TACGGGCAGA AGACCACCCA CTTCCACTGC AGGCGCCCGG GGTGCACCTT CACCTTCAAG 
AACAAGTGCG ACATTGAGAA GCACAAGAGC TACCACATCA AGGATGACGC ATATGCCAAG 
GATGGCTTCA AGAAGTTCTA CAAGTATGAG GAGTGCAAAT ACGAGGGCTG TGTCTACAGC 
AAGGCCACCA ACCACTTCCA CTGCATCCGC TCAAGCTGCG GCTTCACCTT CACCTCCACC 
AGCCAGATGA CCTCCCACAA GCGCAAGCAC GAGCGCCGCC ACATCCGCTC CTCGGGCGTG 
ATGGCCCTCT CCTCTGGCGG CTCCTTCCTG ATGCCCAAGG AGGAACCCGG AGACGAGTCG 
AGCAACGACG ACCTCATGGA CTTCTCGGCC ATCAGCAGCA AGAACTCCAG CCTGAGCGCC 
TCGCCCACCA CCCAGCAGTC GTCCAACGGT CCCGGCACCC ACTTGCTTGC CACACCCACC 
ACGGTGATGT CGTCCTCCGG CGGTGGCCAC AGCGGCCTCA AGCCCACCTC CTCGCTGCCG 
CACTCGGCAC GCATGTCAAG CCTGCTATCC CAGGCGCTGC CCAGCAACAT GCCTGTGGCA 
CTGGCGCTGT CCAACAGTGC CCTCGCCAGC GTCAACAATC CCTTCTTCCC GCTCATGCCC 
CGGCTGCCCA TGCAGCTGCC CCATTCGGCC GCCAGCCTGA TCTCGGCCGT GTCGTCTGGC 
GCCCACTCCA TGCCCACCGA CTCACTGTCC CAGGGCGGCT GCTCCTCGGC GGGCAGCGAT 
GGCGCCATGG CATCCACGCC GACCTCCTAC GCCTCAGCCT CTTCCATCAT GGAGAAGATC 
TCTGCTAGCA AGGGCCTCAT ATCCCCGATG ATGGCCAGGC TGGCAGCAGC TGCCCTCAAG 
CCCTCTGGCA ACCACCAAGA CACAGGGAAT GGGCTGGCTG GTTCTTCAGC GAGTCTGTTC 
AATCTGGTTC AAGTGAAGCA GGAGCCAGTC GACCATACCT CCGGGGCCTC CCAGGACTCC 
ATCCAAGAGC ACAGCCTGGA CCTGAGCAAA AAAGACCACA GTAATGAATC AAACGGACAC 
CCTGTACCGG GAAATACATC TCTTTTATCC TCGCTTATGA ATAAGATGAA TGCCGGCTTC 
TTCAGTGCCC TGAACCTGAA GACGGAGATG GAGATCGGCC AAGGGGCCGA CACCTCAGAG 
GCAGCACAGT ACCTGAGCCG CATCATGAAG AGGCCCATGC CAGACAAACC AAGCGAGCTC 
TGGAGGACAT ATCTGCGCAG GTTTGACACG GAGGACTTCT GCGAGGCCCA GTGTGACTTC 
CTGCAGAAGG TCCACTTCCA TTGTCTAGTG GAGGACTGCG GGGCGCTCTT CAGCACGATG 
GACGGAGCCA TTAAACACGC CAACTTCCAC CTACGAGCCA CGCTGAAGGT GAAATCAGAG 
CCTCACTTCA ACAAAGTCAA GGACTGCGGC GACGGAGCAT CCCACCAGTC CACCGCCCCT 
GTCTCCATGG CTAACAACCC CGCCATGGAC GTGGCCAACA TGTCATCCTC GTCAGGCGGC 
GGCTATGGCT CCTCCCCCTC ACTGCTGGCA TGGAAGCAGC TGACGGGCAG CATCCCACAG 
CTCAATGCCT CCATGCCTAA CCTGCCTGCC AATTCGCCGC TGGCCACCAC CTCTCTGGAG 
AACGCCAAGC CACAGGTCAA ACCGGGCTTC CTGCAGTTCC AGGACAATTC ATACAGTGAC 
CCTTGCCTGG CCACTGACTG TAAGTACTCC AACAAGGTCC ACTTCCACTG TCTCTTCGGG 
AACTGCAAGT ACGTCTGCAA GACCTCGGGG AAGGCCGAAT CCCACTGCTT GGACCACATC 
AATCCTAACA ACCACCTGGT CAACGTTCGA GACCAGTTCT CCTACTTCTC CCTCCAGTGT 
CTATGTCCCA ACCAGCACTG TGAGTTCAGA ATGAGGGGCC ATTACCACTG TCTGCGATCC 
GGCTGCTACT TTGTCACCAA CATCACCACC AAGCTTCCCT GGCATGTCAA GAAGCATGAG 
AAGGCAGAGA GACGCGCCGC CAATGGCTTC AAATACTTCA CCAAGAGAGA GGAGTGTGGC 
AGGCTAGGCT GCAAGTACAA CCAGGTGAAC AGTCACTTCC ACTGCATCCG CGAGAGCTGC 
CAGTTCTCCT TCCTGCTCAA GCACCAGATG ACCTCGCACT CCCGCAAACA CATGAGGAGA 
ATGCTGGGAA AGAACTTTGA CAGGGTTCCA TCCCAGGTGG TGTCCCATGG CCAGAGGGCC 
GATGACATGC AGCACTTGTC GGGCATGGGC TCAGGTCTCA TGGGCAGCCA CCCGGGGATG 
ACCCATGGGT TCTCCAGCAT GATGGATGAG ACGGATGACT ACATGGACTA CATGGGCGGG 
GGCAGCCCCC TGTGCCTTTC CTCTGAGTCC TCCAATCAGG ACCGCAGTTG CAGCAGCACC 
CCCGTGGGCA ATGAAAGCTC CCCAGCAGGA CCAGGCTGCC CGGTCACTAC TGCTCCCTCT 
ACCCCCGCTG ACACTAGCGC TCCCCAAAAC GCACCTCCTC CACCACCTCC TCCTCAGCCT 
GGACTTCAGT CTCAGGCTCC ATCTTTCCCT CCTGCCCTCC TCCGACCTCC TCTTCCCTCG 
CTCCCATATC TCGTCTCTCC ATCCTGTCTG TCATACTCTC TGCTCAGCGC CTCTCTCGGA 
GCCACTCGGA GTGTTGTCAT GCCAACCAAC ACACCAGCTT TCAGCCCCAT CATTGCCACT 
CCGTCTCCGG TTAAAAATGA CGTCCCTATA GTGCAGGATG CTGCAGGCAA CACCATCTCC 
ATCCCTACGG CCACCGGCGC CAAGAAACGC TTCTGGATCA TCGAGGACAT GTCCCCCTTC 
GGCAAGCGGC GTAAGACGGC TTCTGCTCGC AAAATGCTGG ACGAGGGCAT GATGCTGGAG 
GGCTTCCGCC GTTATGATCT GTACGAGGAC TGCAAGGACT CCGGCTGCCA GTTCTCTCTG 
AAGGTGACCC ACTACCACTG CACCCGCGAG AACTGCGGCT ACAAGTTCTG CGGCCGCACG 
CACATGTACA AGCATGCGCA GCACCACGAC CGCGTGGACA ACCTGGTCCT GGACGACTTC 
AAGCGTTTCA AGTCATCGCT CAGCTGCAAC TTCTCCGACT GCCAGTTCTC CGGCAACAGC 
ACCCACTTCC ACTGCCTGCG CTGCAGCTTC CGTTGCACGG ACAGCACCAA GGTGACTGCC 
CACCGCAAAC ACCATGGCAA GCAAGATGTG ATCAGCGCCG CTGGCTTCTG CCAGTTCAGC 
TCCAGCGCTG ACTGCGAGGT TGTCGACTGC AAATACAAGC TCAAGTGCTC ACACTTTCAC 
TGCACCTACC CCGAGTGCAA GCACACTGTA GTCGGTATGT CCCAGATGGA CTCCCACAAG 
CGCAAGCATG AGAAGCAGGA GCGCGGCGAG CTGCCGTCGG TGTCTCCCCG TCAGGAGGGG 
GCGCACCACA TGACGGGAGG AGTGTCCGCA ATGCCCCACG CCTCCATGGG CCTTCTTCCA 
GCCTCGCCCG GTGGCCTCCC AGGACTCTCC CACCACCACC ATCACCACCA TAACAACAAC 
AACCATGCCC CCTCCATGCT CTACCCCCCA GCCAGCGTGG CATCTGACTA CAACCACCCC 
TATCCAACCT CCTTGGTCAG TCTGGACAGC TCCCTCAACC TGGGCACCGA CACCAGCAGC 
TCCCTGTTCT TCCTGAAGAA CGCCGCTGGC CTGGGCCTCA ATGACTCGTT GGACCTGAGC 
AAAAAGATGC AGCATCACCA TGACCCGAGC CTGACCATGG CTGCCGGGCC CAACCCCATG 
CCACCGCTGG GCTTGCCCGC TCCTCAGGAT GACACCACGG GAACATCGGG CGACCCCGAA 
GATGACCAGT CACCCGAGGA AGAGGCCAAT GCAGAGGAAG ATGAGGAGGA TGAGGATGAG 
GCGGAGGAGG ATCTGAACAC TGATTCGTAC GAAGATTCGA TGCCACAGCC CGACGGCGAC 
AACAAGGACA ATGACGAGAG TTTTGACGCT TCCTTAAACC ACACTGACAC TTCCCAACTG 
GAAAAGCAAG AGGCCGACCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

casz1 ( XM_021616108.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021471783.1 Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X4, mRNA.

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CloneID	OOf07777
Clone ID Related Accession (Same CDS sequence)	XM_021616108.1
Accession Version	XM_021616108.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	zinc finger protein castor homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTA CATGTCTGAG AGGATTTAAC TTCGACTTCC CTGAATATGC TGAAAGGACT 
TTATGCACTG AAAAGACCTC AGGAAAATCC AACATGGCCG GCAAAAGGAA AGGTGGCCTA 
AAACTCAACG CAATCTGTGC CAAGCTGAGC CGTCAGGTGG TGTACGACGG CAGCTCCCAG 
CATGCTGAGG GAGACCACAG TGTGGCGGAG AACAGCGAGC GCGGGAGCTC CCATTATGAC 
GACAGCGGCG AGCGATTCGA GACCAACTTC CCCGAGGGCC TGAGTCTCGG CCAGAGCCTC 
GAAGAGGACC AGAAACGGCG CAAGGCCATT GAGAAGTGGG TCAACGGGGA GTATGGGGAT 
GAACCACCAC CCTCTGCCCT GGACTGTGGG GAGGAACAGC AGCAACAGCA GGGACTCAAG 
CCCAACAATG GCGAGGACGG ACCCCCCGAG GGTGTGTACA TGGTACAGCC CAAGGGCTGT 
AGTGACGAGG AGGACAATGC GCTTGATGAG CCCGAACCCA TGTTCGACTC TCGGGACGGC 
AGTTACCACG ACGACAAGGA CTCGGAAGAC GGGCCTCAAA AGGACGTCTA CATGCCGTTG 
ATAAGTGAGC CCCCGAGTCG CCAAACATCT TTCTCCTCTC CAGGAGAGGC GTCCACTCTG 
CGAGACTACG CAGCCAACAC AATGAACGAG TTCCTGGGCA TGTTCGGCTA CGACGACCAG 
CAGGTGAGGG ACGAGCTGGC CAAGAAGATC AGCTTCGAGA AGCTCAAAGC CGCTACCTCA 
GGCTCCGACC CATCCTCCCT GAGCAGCGAG GAGGCCACGC GGCGAGCCCG CTTTTCCAAG 
TATGAGGAGT ACATCCGCAA GCTGAAGGCT GGCGAGACCC TGCCCTGGCC CATCCACACC 
AACCCCAAAC AAGAGGACCT CAACTCCAAG CTTGCCCAGG AGAAGAATGC CCTGCTCCAG 
GCCCAGATGG CCGCCTCTGG GGGGTGCCAT TCCGGGGCTG AGGGACAGAT TTATCACTCC 
AGCCTGGACC ACAAGCAGGG GCAAGGGCAG GGGCACAACC TTGGGCACAA TCCCCCACCA 
ACCAACCCCA CCTCCCACCT CCAGAACATG GCCTCCCGAG CCTCTAAGTA TGACTTCTTC 
ATCCAGAAGC TGAAGATGGG TGAAAGCCTG CGGAGCCAGC AGAATGGCAA TGCTTACAAG 
AAGCCATCCA AGTACGACCT GGAGAATGTT CGATACCTGC ACCTCTTCAA GCCCGGCGAC 
GGGAACACTG ACATGGGGGG CGCCATCGCC TATAAAACGG GGAAGGTGGG CCGCCCGTCC 
AAGTATGATA TCAAGAACAT CCAGAAGCTG ATGCCGGGGA GGCCCGAGTC CTCGCTGATG 
CCCAGCGTTC TGCCTGCCAC ACCTGGGAAC CCGGGGACAC CTGTGGCTGC CACCTCGGCC 
TCCTCCGCTG GGGCCACGGG CCCCCCAGGA CTCATCATGG ATCAGACAGG CCACTTGGGC 
TTCAACACCT CCGACTACAT GAAGTCCAGC TTCTCCAAGA CCGACTCCAT CACCACGGGC 
ACTGTGTCTT CTGTCAAGAA CGGCTTACCA CCTGAGAAAC CTGTCACAGA GGACGTCAAC 
CTGTACCAGA AATATATCGC CAGAAGCCGT CAAACCTTCC CGAAGCCCCT ACCACCACCA 
CCACCACCAC CATACCCTGA GCCCCCCACC ACACCACCAA CTTTTAAAAG GTTCTCGGGG 
AGCCAGCACT GCGGACATGT GCATTGTGCC TACCAATACA GGGAGCATTA CCACTGCATG 
GACACGGAGT GCAACTACCA GGTGGGCTGC AACAAGGTAT ACACCAGCAC CTCCGATGTC 
ATGACTCACG AGAACTTCCA TAAGAAGAAC GCCCAGCTGA TCAACGATGG CTTTCAGAGG 
TTCCGCGCCA CTGAGGATTG TGGGACGGTG GAGTGCCAAT TCTACGGGCA GAAGACCACC 
CACTTCCACT GCAGGCGCCC GGGGTGCACC TTCACCTTCA AGAACAAGTG CGACATTGAG 
AAGCACAAGA GCTACCACAT CAAGGATGAC GCATATGCCA AGGATGGCTT CAAGAAGTTC 
TACAAGTATG AGGAGTGCAA ATACGAGGGC TGTGTCTACA GCAAGGCCAC CAACCACTTC 
CACTGCATCC GCTCAAGCTG CGGCTTCACC TTCACCTCCA CCAGCCAGAT GACCTCCCAC 
AAGCGCAAGC ACGAGCGCCG CCACATCCGC TCCTCGGGCG TGATGGCCCT CTCCTCTGGC 
GGCTCCTTCC TGATGCCCAA GGAGGAACCC GGAGACGAGT CGAGCAACGA CGACCTCATG 
GACTTCTCGG CCATCAGCAG CAAGAACTCC AGCCTGAGCG CCTCGCCCAC CACCCAGCAG 
TCGTCCAACG GTCCCGGCAC CCACTTGCTT GCCACACCCA CCACGGTGAT GTCGTCCTCC 
GGCGGTGGCC ACAGCGGCCT CAAGCCCACC TCCTCGCTGC CGCACTCGGC ACGCATGTCA 
AGCCTGCTAT CCCAGGCGCT GCCCAGCAAC ATGCCTGTGG CACTGGCGCT GTCCAACAGT 
GCCCTCGCCA GCGTCAACAA TCCCTTCTTC CCGCTCATGC CCCGGCTGCC CATGCAGCTG 
CCCCATTCGG CCGCCAGCCT GATCTCGGCC GTGTCGTCTG GCGCCCACTC CATGCCCACC 
GACTCACTGT CCCAGGGCGG CTGCTCCTCG GCGGGCAGCG ATGGCGCCAT GGCATCCACG 
CCGACCTCCT ACGCCTCAGC CTCTTCCATC ATGGAGAAGA TCTCTGCTAG CAAGGGCCTC 
ATATCCCCGA TGATGGCCAG GCTGGCAGCA GCTGCCCTCA AGCCCTCTGG CAACCACCAA 
GACACAGGGA ATGGGCTGGC TGGTTCTTCA GCGAGTCTGT TCAATCTGGT TCAAGTGAAG 
CAGGAGCCAG TCGACCATAC CTCCGGGGCC TCCCAGGACT CCATCCAAGA GCACAGCCTG 
GACCTGAGCA AAAAAGACCA CAGTAATGAA TCAAACGGAC ACCCTGTACC GGGAAATACA 
TCTCTTTTAT CCTCGCTTAT GAATAAGATG AATGCCGGCT TCTTCAGTGC CCTGAACCTG 
AAGACGGAGA TGGAGATCGG CCAAGGGGCC GACACCTCAG AGGCAGCACA GTACCTGAGC 
CGCATCATGA AGAGGCCCAT GCCAGACAAA CCAAGCGAGC TCTGGAGGAC ATATCTGCGC 
AGGTTTGACA CGGAGGACTT CTGCGAGGCC CAGTGTGACT TCCTGCAGAA GGTCCACTTC 
CATTGTCTAG TGGAGGACTG CGGGGCGCTC TTCAGCACGA TGGACGGAGC CATTAAACAC 
GCCAACTTCC ACCTACGAGC CACGCTGAAG GTGAAATCAG AGCCTCACTT CAACAAAGTC 
AAGGACTGCG GCGACGGAGC ATCCCACCAG TCCACCGCCC CTGTCTCCAT GGCTAACAAC 
CCCGCCATGG ACGTGGCCAA CATGTCATCC TCGTCAGGCG GCGGCTATGG CTCCTCCCCC 
TCACTGCTGG CATGGAAGCA GCTGACGGGC AGCATCCCAC AGCTCAATGC CTCCATGCCT 
AACCTGCCTG CCAATTCGCC GCTGGCCACC ACCTCTCTGG AGAACGCCAA GCCACAGGTC 
AAACCGGGCT TCCTGCAGTT CCAGGACAAT TCATACAGTG ACCCTTGCCT GGCCACTGAC 
TGTAAGTACT CCAACAAGGT CCACTTCCAC TGTCTCTTCG GGAACTGCAA GTACGTCTGC 
AAGACCTCGG GGAAGGCCGA ATCCCACTGC TTGGACCACA TCAATCCTAA CAACCACCTG 
GTCAACGTTC GAGACCAGTT CTCCTACTTC TCCCTCCAGT GTCTATGTCC CAACCAGCAC 
TGTGAGTTCA GAATGAGGGG CCATTACCAC TGTCTGCGAT CCGGCTGCTA CTTTGTCACC 
AACATCACCA CCAAGCTTCC CTGGCATGTC AAGAAGCATG AGAAGGCAGA GAGACGCGCC 
GCCAATGGCT TCAAATACTT CACCAAGAGA GAGGAGTGTG GCAGGCTAGG CTGCAAGTAC 
AACCAGGTGA ACAGTCACTT CCACTGCATC CGCGAGAGCT GCCAGTTCTC CTTCCTGCTC 
AAGCACCAGA TGACCTCGCA CTCCCGCAAA CACATGAGGA GAATGCTGGG AAAGAACTTT 
GACAGGGTTC CATCCCAGGT GGTGTCCCAT GGCCAGAGGG CCGATGACAT GCAGCACTTG 
TCGGGCATGG GCTCAGGTCT CATGGGCAGC CACCCGGGGA TGACCCATGG GTTCTCCAGC 
ATGATGGATG AGACGGATGA CTACATGGAC TACATGGGCG GGGGCAGCCC CCTGTGCCTT 
TCCTCTGAGT CCTCCAATCA GGACCGCAGT TGCAGCAGCA CCCCCGTGGG CAATGAAAGC 
TCCCCAGCAG GACCAGGCTG CCCGGTCACT ACTGCTCCCT CTACCCCCGC TGACACTAGC 
GCTCCCCAAA ACGCACCTCC TCCACCACCT CCTCCTCAGC CTGGACTTCA GTCTCAGGCT 
CCATCTTTCC CTCCTGCCCT CCTCCGACCT CCTCTTCCCT CGCTCCCATA TCTCGTCTCT 
CCATCCTGTC TGTCATACTC TCTGCTCAGC GCCTCTCTCG GAGCCACTCG GAGTGTTGTC 
ATGCCAACCA ACACACCAGC TTTCAGCCCC ATCATTGCCA CTCCGTCTCC GGTTAAAAAT 
GACGTCCCTA TAGTGCAGGA TGCTGCAGGC AACACCATCT CCATCCCTAC GGCCACCGGC 
GCCAAGAAAC GCTTCTGGAT CATCGAGGAC ATGTCCCCCT TCGGCAAGCG GCGTAAGACG 
GCTTCTGCTC GCAAAATGCT GGACGAGGGC ATGATGCTGG AGGGCTTCCG CCGTTATGAT 
CTGTACGAGG ACTGCAAGGA CTCCGGCTGC CAGTTCTCTC TGAAGGTGAC CCACTACCAC 
TGCACCCGCG AGAACTGCGG CTACAAGTTC TGCGGCCGCA CGCACATGTA CAAGCATGCG 
CAGCACCACG ACCGCGTGGA CAACCTGGTC CTGGACGACT TCAAGCGTTT CAAGTCATCG 
CTCAGCTGCA ACTTCTCCGA CTGCCAGTTC TCCGGCAACA GCACCCACTT CCACTGCCTG 
CGCTGCAGCT TCCGTTGCAC GGACAGCACC AAGGTGACTG CCCACCGCAA ACACCATGGC 
AAGCAAGATG TGATCAGCGC CGCTGGCTTC TGCCAGTTCA GCTCCAGCGC TGACTGCGAG 
GTTGTCGACT GCAAATACAA GCTCAAGTGC TCACACTTTC ACTGCACCTA CCCCGAGTGC 
AAGCACACTG TAGTCGGTAT GTCCCAGATG GACTCCCACA AGCGCAAGCA TGAGAAGCAG 
GAGCGCGGCG AGCTGCCGTC GGTGTCTCCC CGTCAGGAGG GGGCGCACCA CATGACGGGA 
GGAGTGTCCG CAATGCCCCA CGCCTCCATG GGCCTTCTTC CAGCCTCGCC CGGTGGCCTC 
CCAGGACTCT CCCACCACCA CCATCACCAC CATAACAACA ACAACCATGC CCCCTCCATG 
CTCTACCCCC CAGCCAGCGT GGCATCTGAC TACAACCACC CCTATCCAAC CTCCTTGGTC 
AGTCTGGACA GCTCCCTCAA CCTGGGCACC GACACCAGCA GCTCCCTGTT CTTCCTGAAG 
AACGCCGCTG GCCTGGGCCT CAATGACTCG TTGGACCTGA GCAAAAAGAT GCAGCATCAC 
CATGACCCGA GCCTGACCAT GGCTGCCGGG CCCAACCCCA TGCCACCGCT GGGCTTGCCC 
GCTCCTCAGG ATGACACCAC GGGAACATCG GGCGACCCCG AAGATGACCA GTCACCCGAG 
GAAGAGGCCA ATGCAGAGGA AGATGAGGAG GATGAGGATG AGGCGGAGGA GGATCTGAAC 
ACTGATTCGT ACGAAGATTC GATGCCACAG CCCGACGGCG ACAACAAGGA CAATGACGAG 
AGTTTTGACG CTTCCTTAAA CCACACTGAC ACTTCCCAAC TGGAAAAGCA AGAGGCCGAC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021471783.1
CDS	355..6420
Translation	MKVTCLRGFNFDFPEYAERTLCTEKTSGKSNMAGKRKGGLKLNAICAKLSRQVVYDGSSQHAEGDHSVAENSERGSSHYDDSGERFETNFPEGLSLGQSLEEDQKRRKAIEKWVNGEYGDEPPPSALDCGEEQQQQQGLKPNNGEDGPPEGVYMVQPKGCSDEEDNALDEPEPMFDSRDGSYHDDKDSEDGPQKDVYMPLISEPPSRQTSFSSPGEASTLRDYAANTMNEFLGMFGYDDQQVRDELAKKISFEKLKAATSGSDPSSLSSEEATRRARFSKYEEYIRKLKAGETLPWPIHTNPKQEDLNSKLAQEKNALLQAQMAASGGCHSGAEGQIYHSSLDHKQGQGQGHNLGHNPPPTNPTSHLQNMASRASKYDFFIQKLKMGESLRSQQNGNAYKKPSKYDLENVRYLHLFKPGDGNTDMGGAIAYKTGKVGRPSKYDIKNIQKLMPGRPESSLMPSVLPATPGNPGTPVAATSASSAGATGPPGLIMDQTGHLGFNTSDYMKSSFSKTDSITTGTVSSVKNGLPPEKPVTEDVNLYQKYIARSRQTFPKPLPPPPPPPYPEPPTTPPTFKRFSGSQHCGHVHCAYQYREHYHCMDTECNYQVGCNKVYTSTSDVMTHENFHKKNAQLINDGFQRFRATEDCGTVECQFYGQKTTHFHCRRPGCTFTFKNKCDIEKHKSYHIKDDAYAKDGFKKFYKYEECKYEGCVYSKATNHFHCIRSSCGFTFTSTSQMTSHKRKHERRHIRSSGVMALSSGGSFLMPKEEPGDESSNDDLMDFSAISSKNSSLSASPTTQQSSNGPGTHLLATPTTVMSSSGGGHSGLKPTSSLPHSARMSSLLSQALPSNMPVALALSNSALASVNNPFFPLMPRLPMQLPHSAASLISAVSSGAHSMPTDSLSQGGCSSAGSDGAMASTPTSYASASSIMEKISASKGLISPMMARLAAAALKPSGNHQDTGNGLAGSSASLFNLVQVKQEPVDHTSGASQDSIQEHSLDLSKKDHSNESNGHPVPGNTSLLSSLMNKMNAGFFSALNLKTEMEIGQGADTSEAAQYLSRIMKRPMPDKPSELWRTYLRRFDTEDFCEAQCDFLQKVHFHCLVEDCGALFSTMDGAIKHANFHLRATLKVKSEPHFNKVKDCGDGASHQSTAPVSMANNPAMDVANMSSSSGGGYGSSPSLLAWKQLTGSIPQLNASMPNLPANSPLATTSLENAKPQVKPGFLQFQDNSYSDPCLATDCKYSNKVHFHCLFGNCKYVCKTSGKAESHCLDHINPNNHLVNVRDQFSYFSLQCLCPNQHCEFRMRGHYHCLRSGCYFVTNITTKLPWHVKKHEKAERRAANGFKYFTKREECGRLGCKYNQVNSHFHCIRESCQFSFLLKHQMTSHSRKHMRRMLGKNFDRVPSQVVSHGQRADDMQHLSGMGSGLMGSHPGMTHGFSSMMDETDDYMDYMGGGSPLCLSSESSNQDRSCSSTPVGNESSPAGPGCPVTTAPSTPADTSAPQNAPPPPPPPQPGLQSQAPSFPPALLRPPLPSLPYLVSPSCLSYSLLSASLGATRSVVMPTNTPAFSPIIATPSPVKNDVPIVQDAAGNTISIPTATGAKKRFWIIEDMSPFGKRRKTASARKMLDEGMMLEGFRRYDLYEDCKDSGCQFSLKVTHYHCTRENCGYKFCGRTHMYKHAQHHDRVDNLVLDDFKRFKSSLSCNFSDCQFSGNSTHFHCLRCSFRCTDSTKVTAHRKHHGKQDVISAAGFCQFSSSADCEVVDCKYKLKCSHFHCTYPECKHTVVGMSQMDSHKRKHEKQERGELPSVSPRQEGAHHMTGGVSAMPHASMGLLPASPGGLPGLSHHHHHHHNNNNHAPSMLYPPASVASDYNHPYPTSLVSLDSSLNLGTDTSSSLFFLKNAAGLGLNDSLDLSKKMQHHHDPSLTMAAGPNPMPPLGLPAPQDDTTGTSGDPEDDQSPEEEANAEEDEEDEDEAEEDLNTDSYEDSMPQPDGDNKDNDESFDASLNHTDTSQLEKQEADP

Target ORF information:

RefSeq Version	XM_021616108.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss castor zinc finger 1 (casz1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021616108.1

ORF Insert Sequence:

ATGAAAGTTA CATGTCTGAG AGGATTTAAC TTCGACTTCC CTGAATATGC TGAAAGGACT 
TTATGCACTG AAAAGACCTC AGGAAAATCC AACATGGCCG GCAAAAGGAA AGGTGGCCTA 
AAACTCAACG CAATCTGTGC CAAGCTGAGC CGTCAGGTGG TGTACGACGG CAGCTCCCAG 
CATGCTGAGG GAGACCACAG TGTGGCGGAG AACAGCGAGC GCGGGAGCTC CCATTATGAC 
GACAGCGGCG AGCGATTCGA GACCAACTTC CCCGAGGGCC TGAGTCTCGG CCAGAGCCTC 
GAAGAGGACC AGAAACGGCG CAAGGCCATT GAGAAGTGGG TCAACGGGGA GTATGGGGAT 
GAACCACCAC CCTCTGCCCT GGACTGTGGG GAGGAACAGC AGCAACAGCA GGGACTCAAG 
CCCAACAATG GCGAGGACGG ACCCCCCGAG GGTGTGTACA TGGTACAGCC CAAGGGCTGT 
AGTGACGAGG AGGACAATGC GCTTGATGAG CCCGAACCCA TGTTCGACTC TCGGGACGGC 
AGTTACCACG ACGACAAGGA CTCGGAAGAC GGGCCTCAAA AGGACGTCTA CATGCCGTTG 
ATAAGTGAGC CCCCGAGTCG CCAAACATCT TTCTCCTCTC CAGGAGAGGC GTCCACTCTG 
CGAGACTACG CAGCCAACAC AATGAACGAG TTCCTGGGCA TGTTCGGCTA CGACGACCAG 
CAGGTGAGGG ACGAGCTGGC CAAGAAGATC AGCTTCGAGA AGCTCAAAGC CGCTACCTCA 
GGCTCCGACC CATCCTCCCT GAGCAGCGAG GAGGCCACGC GGCGAGCCCG CTTTTCCAAG 
TATGAGGAGT ACATCCGCAA GCTGAAGGCT GGCGAGACCC TGCCCTGGCC CATCCACACC 
AACCCCAAAC AAGAGGACCT CAACTCCAAG CTTGCCCAGG AGAAGAATGC CCTGCTCCAG 
GCCCAGATGG CCGCCTCTGG GGGGTGCCAT TCCGGGGCTG AGGGACAGAT TTATCACTCC 
AGCCTGGACC ACAAGCAGGG GCAAGGGCAG GGGCACAACC TTGGGCACAA TCCCCCACCA 
ACCAACCCCA CCTCCCACCT CCAGAACATG GCCTCCCGAG CCTCTAAGTA TGACTTCTTC 
ATCCAGAAGC TGAAGATGGG TGAAAGCCTG CGGAGCCAGC AGAATGGCAA TGCTTACAAG 
AAGCCATCCA AGTACGACCT GGAGAATGTT CGATACCTGC ACCTCTTCAA GCCCGGCGAC 
GGGAACACTG ACATGGGGGG CGCCATCGCC TATAAAACGG GGAAGGTGGG CCGCCCGTCC 
AAGTATGATA TCAAGAACAT CCAGAAGCTG ATGCCGGGGA GGCCCGAGTC CTCGCTGATG 
CCCAGCGTTC TGCCTGCCAC ACCTGGGAAC CCGGGGACAC CTGTGGCTGC CACCTCGGCC 
TCCTCCGCTG GGGCCACGGG CCCCCCAGGA CTCATCATGG ATCAGACAGG CCACTTGGGC 
TTCAACACCT CCGACTACAT GAAGTCCAGC TTCTCCAAGA CCGACTCCAT CACCACGGGC 
ACTGTGTCTT CTGTCAAGAA CGGCTTACCA CCTGAGAAAC CTGTCACAGA GGACGTCAAC 
CTGTACCAGA AATATATCGC CAGAAGCCGT CAAACCTTCC CGAAGCCCCT ACCACCACCA 
CCACCACCAC CATACCCTGA GCCCCCCACC ACACCACCAA CTTTTAAAAG GTTCTCGGGG 
AGCCAGCACT GCGGACATGT GCATTGTGCC TACCAATACA GGGAGCATTA CCACTGCATG 
GACACGGAGT GCAACTACCA GGTGGGCTGC AACAAGGTAT ACACCAGCAC CTCCGATGTC 
ATGACTCACG AGAACTTCCA TAAGAAGAAC GCCCAGCTGA TCAACGATGG CTTTCAGAGG 
TTCCGCGCCA CTGAGGATTG TGGGACGGTG GAGTGCCAAT TCTACGGGCA GAAGACCACC 
CACTTCCACT GCAGGCGCCC GGGGTGCACC TTCACCTTCA AGAACAAGTG CGACATTGAG 
AAGCACAAGA GCTACCACAT CAAGGATGAC GCATATGCCA AGGATGGCTT CAAGAAGTTC 
TACAAGTATG AGGAGTGCAA ATACGAGGGC TGTGTCTACA GCAAGGCCAC CAACCACTTC 
CACTGCATCC GCTCAAGCTG CGGCTTCACC TTCACCTCCA CCAGCCAGAT GACCTCCCAC 
AAGCGCAAGC ACGAGCGCCG CCACATCCGC TCCTCGGGCG TGATGGCCCT CTCCTCTGGC 
GGCTCCTTCC TGATGCCCAA GGAGGAACCC GGAGACGAGT CGAGCAACGA CGACCTCATG 
GACTTCTCGG CCATCAGCAG CAAGAACTCC AGCCTGAGCG CCTCGCCCAC CACCCAGCAG 
TCGTCCAACG GTCCCGGCAC CCACTTGCTT GCCACACCCA CCACGGTGAT GTCGTCCTCC 
GGCGGTGGCC ACAGCGGCCT CAAGCCCACC TCCTCGCTGC CGCACTCGGC ACGCATGTCA 
AGCCTGCTAT CCCAGGCGCT GCCCAGCAAC ATGCCTGTGG CACTGGCGCT GTCCAACAGT 
GCCCTCGCCA GCGTCAACAA TCCCTTCTTC CCGCTCATGC CCCGGCTGCC CATGCAGCTG 
CCCCATTCGG CCGCCAGCCT GATCTCGGCC GTGTCGTCTG GCGCCCACTC CATGCCCACC 
GACTCACTGT CCCAGGGCGG CTGCTCCTCG GCGGGCAGCG ATGGCGCCAT GGCATCCACG 
CCGACCTCCT ACGCCTCAGC CTCTTCCATC ATGGAGAAGA TCTCTGCTAG CAAGGGCCTC 
ATATCCCCGA TGATGGCCAG GCTGGCAGCA GCTGCCCTCA AGCCCTCTGG CAACCACCAA 
GACACAGGGA ATGGGCTGGC TGGTTCTTCA GCGAGTCTGT TCAATCTGGT TCAAGTGAAG 
CAGGAGCCAG TCGACCATAC CTCCGGGGCC TCCCAGGACT CCATCCAAGA GCACAGCCTG 
GACCTGAGCA AAAAAGACCA CAGTAATGAA TCAAACGGAC ACCCTGTACC GGGAAATACA 
TCTCTTTTAT CCTCGCTTAT GAATAAGATG AATGCCGGCT TCTTCAGTGC CCTGAACCTG 
AAGACGGAGA TGGAGATCGG CCAAGGGGCC GACACCTCAG AGGCAGCACA GTACCTGAGC 
CGCATCATGA AGAGGCCCAT GCCAGACAAA CCAAGCGAGC TCTGGAGGAC ATATCTGCGC 
AGGTTTGACA CGGAGGACTT CTGCGAGGCC CAGTGTGACT TCCTGCAGAA GGTCCACTTC 
CATTGTCTAG TGGAGGACTG CGGGGCGCTC TTCAGCACGA TGGACGGAGC CATTAAACAC 
GCCAACTTCC ACCTACGAGC CACGCTGAAG GTGAAATCAG AGCCTCACTT CAACAAAGTC 
AAGGACTGCG GCGACGGAGC ATCCCACCAG TCCACCGCCC CTGTCTCCAT GGCTAACAAC 
CCCGCCATGG ACGTGGCCAA CATGTCATCC TCGTCAGGCG GCGGCTATGG CTCCTCCCCC 
TCACTGCTGG CATGGAAGCA GCTGACGGGC AGCATCCCAC AGCTCAATGC CTCCATGCCT 
AACCTGCCTG CCAATTCGCC GCTGGCCACC ACCTCTCTGG AGAACGCCAA GCCACAGGTC 
AAACCGGGCT TCCTGCAGTT CCAGGACAAT TCATACAGTG ACCCTTGCCT GGCCACTGAC 
TGTAAGTACT CCAACAAGGT CCACTTCCAC TGTCTCTTCG GGAACTGCAA GTACGTCTGC 
AAGACCTCGG GGAAGGCCGA ATCCCACTGC TTGGACCACA TCAATCCTAA CAACCACCTG 
GTCAACGTTC GAGACCAGTT CTCCTACTTC TCCCTCCAGT GTCTATGTCC CAACCAGCAC 
TGTGAGTTCA GAATGAGGGG CCATTACCAC TGTCTGCGAT CCGGCTGCTA CTTTGTCACC 
AACATCACCA CCAAGCTTCC CTGGCATGTC AAGAAGCATG AGAAGGCAGA GAGACGCGCC 
GCCAATGGCT TCAAATACTT CACCAAGAGA GAGGAGTGTG GCAGGCTAGG CTGCAAGTAC 
AACCAGGTGA ACAGTCACTT CCACTGCATC CGCGAGAGCT GCCAGTTCTC CTTCCTGCTC 
AAGCACCAGA TGACCTCGCA CTCCCGCAAA CACATGAGGA GAATGCTGGG AAAGAACTTT 
GACAGGGTTC CATCCCAGGT GGTGTCCCAT GGCCAGAGGG CCGATGACAT GCAGCACTTG 
TCGGGCATGG GCTCAGGTCT CATGGGCAGC CACCCGGGGA TGACCCATGG GTTCTCCAGC 
ATGATGGATG AGACGGATGA CTACATGGAC TACATGGGCG GGGGCAGCCC CCTGTGCCTT 
TCCTCTGAGT CCTCCAATCA GGACCGCAGT TGCAGCAGCA CCCCCGTGGG CAATGAAAGC 
TCCCCAGCAG GACCAGGCTG CCCGGTCACT ACTGCTCCCT CTACCCCCGC TGACACTAGC 
GCTCCCCAAA ACGCACCTCC TCCACCACCT CCTCCTCAGC CTGGACTTCA GTCTCAGGCT 
CCATCTTTCC CTCCTGCCCT CCTCCGACCT CCTCTTCCCT CGCTCCCATA TCTCGTCTCT 
CCATCCTGTC TGTCATACTC TCTGCTCAGC GCCTCTCTCG GAGCCACTCG GAGTGTTGTC 
ATGCCAACCA ACACACCAGC TTTCAGCCCC ATCATTGCCA CTCCGTCTCC GGTTAAAAAT 
GACGTCCCTA TAGTGCAGGA TGCTGCAGGC AACACCATCT CCATCCCTAC GGCCACCGGC 
GCCAAGAAAC GCTTCTGGAT CATCGAGGAC ATGTCCCCCT TCGGCAAGCG GCGTAAGACG 
GCTTCTGCTC GCAAAATGCT GGACGAGGGC ATGATGCTGG AGGGCTTCCG CCGTTATGAT 
CTGTACGAGG ACTGCAAGGA CTCCGGCTGC CAGTTCTCTC TGAAGGTGAC CCACTACCAC 
TGCACCCGCG AGAACTGCGG CTACAAGTTC TGCGGCCGCA CGCACATGTA CAAGCATGCG 
CAGCACCACG ACCGCGTGGA CAACCTGGTC CTGGACGACT TCAAGCGTTT CAAGTCATCG 
CTCAGCTGCA ACTTCTCCGA CTGCCAGTTC TCCGGCAACA GCACCCACTT CCACTGCCTG 
CGCTGCAGCT TCCGTTGCAC GGACAGCACC AAGGTGACTG CCCACCGCAA ACACCATGGC 
AAGCAAGATG TGATCAGCGC CGCTGGCTTC TGCCAGTTCA GCTCCAGCGC TGACTGCGAG 
GTTGTCGACT GCAAATACAA GCTCAAGTGC TCACACTTTC ACTGCACCTA CCCCGAGTGC 
AAGCACACTG TAGTCGGTAT GTCCCAGATG GACTCCCACA AGCGCAAGCA TGAGAAGCAG 
GAGCGCGGCG AGCTGCCGTC GGTGTCTCCC CGTCAGGAGG GGGCGCACCA CATGACGGGA 
GGAGTGTCCG CAATGCCCCA CGCCTCCATG GGCCTTCTTC CAGCCTCGCC CGGTGGCCTC 
CCAGGACTCT CCCACCACCA CCATCACCAC CATAACAACA ACAACCATGC CCCCTCCATG 
CTCTACCCCC CAGCCAGCGT GGCATCTGAC TACAACCACC CCTATCCAAC CTCCTTGGTC 
AGTCTGGACA GCTCCCTCAA CCTGGGCACC GACACCAGCA GCTCCCTGTT CTTCCTGAAG 
AACGCCGCTG GCCTGGGCCT CAATGACTCG TTGGACCTGA GCAAAAAGAT GCAGCATCAC 
CATGACCCGA GCCTGACCAT GGCTGCCGGG CCCAACCCCA TGCCACCGCT GGGCTTGCCC 
GCTCCTCAGG ATGACACCAC GGGAACATCG GGCGACCCCG AAGATGACCA GTCACCCGAG 
GAAGAGGCCA ATGCAGAGGA AGATGAGGAG GATGAGGATG AGGCGGAGGA GGATCTGAAC 
ACTGATTCGT ACGAAGATTC GATGCCACAG CCCGACGGCG ACAACAAGGA CAATGACGAG 
AGTTTTGACG CTTCCTTAAA CCACACTGAC ACTTCCCAAC TGGAAAAGCA AGAGGCCGAC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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casz1 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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