agrn cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	agrn
Entrez Gene ID	110532178
Full Name	agrin
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021615848.1	XP_021471523.1	agrin

Pathways from BioSystems
Gene Ontology

The following agrn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the agrn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf07563	XM_021615848.1 Latest version!	Oncorhynchus mykiss agrin (agrn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

agrn ( XM_021615848.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021471523.1 Oncorhynchus mykiss agrin (agrn), mRNA.

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CloneID	OOf07563
Clone ID Related Accession (Same CDS sequence)	XM_021615848.1
Accession Version	XM_021615848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	agrin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCAC AACGGAGACC AACTCACTTG TACGGGTGGG CATTGATGAT AGTCGCCGTT 
TTCTGTCAAC GATGTCACGG AAGTTGTCCG GAGAAAGACT TGGAGAAAAG GGAAGAGGAG 
GCGAACGTAG TTTTGACTGG TACCGTTGAA GAAATCATGA ACATGGACCC AGTGCATAAT 
ACATACTCGT GCAAGGTGAG GGTATGGCGG TACCTGAAAG GCAAGACCAT GGTCAACGGC 
GAGGTTCTTC TGGACGGCGG CAACAAGGTG ATGATCGGCG GCTTCGGGGA CCCTGTGATA 
TGCGACAACC AGGTTGCCAC TGGCGACACG CGCATTTTCT TTGTCAACCT GGCCCCGGAG 
TACATGTGGC CAGCACATAA GAACGAGCTG ATGCTCAACT CCAGCCTGAT GAGGATTACT 
CTGCGTAACC TGGAGGAGGT GGAGCACTGT GTGGACGACA AACCTGTTCA CTTCACGCCG 
GCCCAGCCTC CTGATGGTTG TAGAGGTATG CTGTGCGGCT TCGGGGCGGT GTGTGAGCGT 
GAAGCGACTG ACCCCTCTAA GGCGGAGTGT GTATGCAAGA AGATGGTTTG TCCGTCGGTT 
GTGGCGCCCG TGTGCGGCTC AGACTCCTCC ACCTACTCCA ACGAGTGCGA GCTGGAGAAG 
GCCCAGTGCA ACACGCAGCG ACGCATCAAG GTGCTGCGCA AGGGACCCTG TGCCCTGAAG 
GATCCATGCA GCGAGGTGAT CTGCAGTTAC GGCAGCACCT GCATCCAGTC ATCGGACGGC 
CTGTCGGCCA AGTGCATGTG CCCGCTGAGC TGCGAAGGCA AGCCCGAGCA GGTGATGTGT 
GGCAGCGATG GCCAGGACTA CCGCAACGAG TGTGAGCTCC ACAAGCAGGC CTGCCATACC 
AAGAAGAACA TCCGCCTTCA GCGCCAGGGA TCCTGCAACC CATGTAAAGG CCTCGAGAAC 
AACCTGAACG TTGCATGCCG TGTAGAACCG CGCACCCACC AGCCCCTGAA TATCCCCCGC 
CCGGAGTCCT GCCCTGCCGT CAATGAGCCA CTGTGTGCCA GCGATGGGCA GACGTACGAG 
AGTGAGTGCC ATATGAAGCG GACGGGCATG CAGAAGGGCA TTGAACTCAA GAAGAACCAC 
CCGGGCCGCT GCAAGAAACA AGAGGAGTGC AGGGAGGAGT GCAAGTTCAA CGGCGTGTGC 
CAGGTGGAGC GTCTGGGCAT GCGCTGCTCC TGCGAGCCCA TCCAGTGCGA TGGCACCTAC 
AAGCCGCTAT GCGGCAAGGA CGGACACACC TACATCAATG ACTGCGAGCG CCGGCGGGCT 
GAGTGCCAGG CCAAGGCCCA TATTCCCGTC AAGCAGCAAG GCCCGTGTGA TCTACACACT 
CCCAGCCCGT GCCTGGGGAA GGCCTGCGAG TTTGGGGCCG CGTGCGTGGT GAAGAACAAC 
GAGCCCGTGT GCGAGTGCCA TGGCGCCTGC CCCCAGATGC CCGACCTGGT GTGCGGCAGT 
GACGGCCACA GCTACGGCAG CCAGTGTGAG ATGAAGGCCA TGGGCTGCGC CCTGCAGAAG 
GAGATTCACA TCCAGCACCG AGGACCCTGC GACGAGGCGT GTGCCAACTG TTCGTTTGGG 
GCGATCTGCG ATGGGCAGTC TGGCCGCTGT GTGTGCCCGC AGGAGTGTGT GGAGTCCCAC 
CAACCGGTGT GCGGCAGCGA CGGCTCCACC TACGACAGCG AGTGTGAGCT CCACGTGCGT 
GCCTGTACCG AGCAGCTTGA CCTGCGCGTG CTCGCCCAGG GAGAATGCAA GACGTGCGGT 
AGCGCCGTGT GTGCCTGGGG GGCCCGCTGC GTCCAGAACA AGTGCGAGTG CCCGCAATGC 
CAGGGCCAGG CCTTCTCGCC CGTGTGCGGC AGCGACGGCT TCACCTACGA CAACACCTGC 
GAGCTGGGCG TGGCCTCCTG CGTCCTCAAA AAGAAGATTG AGGCGGCCAA GCCTGGCAGC 
TGCGACGAAG AGTGTGGTTC AGGAGGATCG GGGTCAGGTA TTGAGAGCTG CGAACAGGAC 
AGGTGTCGTA CGTTTGGAGG ATCCTGGGAC GAGGACGCAG AGGACGACCG CTGTGTCTGT 
GACTTCGACT GCCATCGCGT GCCTCGCAAC CCGGTGTGTG GCTCAAATGG GAAGACCTAC 
AGCAACGAGT GTGAAATGAA GAAGGCTAGG TGTGAAAAGC AGGAGCACCT GCTGATCCAG 
AATCAGGGAC CCTGTACAGC GATTTCGGCC ACCTCTCCGA CAAAACTGCC AGCGCAGGAA 
CACTGCAGTC TGTCAGTCTA TGGCTGTTGC CGTGACAACA AGACGGCGGC CCTTGGTGTG 
GGACTTGCCG GATGCCCCAG TACATGCCAG TGTAACCCGT ACGGCTCCTA CAAGGGGACT 
TGTGACCCGG GCAGCGGCCA GTGCTCCTGT AAGCCCGGCG TCGGGGGACA GAAGTGCGAC 
CGCTGCGATC CAAGCTTCTG GAACTTCCGC GGCATCGTCA CAGAGAACTT GAGCGGCTGC 
ACACCGTGTA ACTGCGACGC GGTGGGCTCG GTGAGGGACG ACTGCGAGCA AATGTCAGGC 
CTGTGCTCCT GCAAGACGGG AGTGAAGGGC ATGAAGTGCA ACGTGTGCCC CGACGGCAGC 
CAGATGGGTA TGAGCGGCTG CGACACAGGT GCCGATGCCC CAACTTCATG TGATGAGCTG 
GACTGCAAGT TTGGCGCGTT GTGTGACGAG GTCAATGGTC AAGCCCACTG CGAATGTCCC 
TCTCCCGACT GTGACGAGAA GAACAAGACC AAGGTGTGTG GCTCTGATGG GGTGACCTAC 
GCTGACCAGT GCCAGCTGAG GACCATTGCC TGTAGGCAGG ACAAGCACAT CACAGTGGAA 
CGCTTTGGCC AGTGTACAGA AAGCATCATA GAGCCGGCCG GCCGATCCAC TCTTTACCCC 
CCCACCTCCC ACGCCGCCGC CACCACCCCC TCCACCGCCC ACGTCAGGCT CACGTCGCCG 
CACTACGATG CATCACTCTC TCCCTGGGAC GATGATCAAA AGAACGGGCT CCCGGCCTCT 
TTCGTGGAAG CCTTGCCACC CCCCGTGGCC ACGGAGAGCG CGCTCACCAC CAACCGCCCC 
GTCACCACCC CCCGCCACCC ACCTCACTCC ACCACCCCCC ACCTGCCTGG CCACACCAGC 
CCTGGCTCCT CCCCTACCGC CTTTGAAGAC TCAGGGAGCG GCGAGCCCAG CGGAGACGAC 
CAGGTGGCGG AAGAGGAGGA GAATGAGGAA GAAGGTAGCG CCTTCGGAGT TCTGGAGGCG 
AGCGGAGAGG AGCCTGTTGA CCCCACAGCT GCGGCCACCA CCATCCCCAC GGCGGAAGAT 
CGCTCGTCGT GTGACAACAC TCCCTTCGGC TGCTGTCCCG ACGGCAAGAC GGCCTCCAGC 
AGCCCTGAGG GAGCCAATTG CCCCCCCACC ATGAGGTTCA GCGGCTTCCT GCACCTAGAC 
CAGGTGGAGG GCCAGGAGGT CTTCTACACC CCTGAGATGG AGGATCCCAA GTCTGAGCTG 
TTTGGGGAGA CGGCCAGGAG CATCGAGAGT GCTCTGAACG AGTTGTTCCG GAAGTCAGAG 
GTGCAGAAAG ACTTCATGAG TGTCCGCGTC CGTAGCCTGG CTCCCAGCAA CTCCATCTTG 
GCCTTCGTCG AGGCCCACTT TGACTCAGAG ACCAAGTACA CAGTGGAGGA CATCGAGGGA 
GCCCTGCTCA AGCAGCTGAA GGCAGCCAAG GAGACAGCCA TCATGGTGAA GAAGCCAGAG 
GAGGAGAACA TTCGCTTCAC CAACTACGGC CTCTCCTCCC TCCCCTACTT CACCACAACC 
ACCACTACAA CGGCCACAGC TACCACCCCC ACCGCCAGCC CCTCCCCCTT CATCACAAGC 
CGTCCCCCGG GCACCACCCG CCGCCCTTAC ACCAGCCGCC GGACCACCAC CGCCCCCTCG 
CCAGTGACCA CCCCGGCCTC CACTACTACC ATCACCCCCC TCCACAGGAC CAGGCCTGTG 
CCCCACCACA CCAAGGCCCA GCGGCCCTGC GACTCCCACC CCTGCCGCCA CGGGGGCACC 
TGCGAGGACG ACGGTGCCGA CTTCACCTGC ATCTGCCCCG CCGGGAGAGG GGGTGCCGTC 
TGTGAGAAAC TGATCAAGTA CTTCATTCCG TCGTTCGGGG GTAAGTCCTA CCTGGCCTTT 
GACACCATGA AGGCTTACCA CACGGTCCGC ATCGCCATGG AATTCAGGGC CGCCGAGATG 
ACGGGCATCC TCCTCTTCAA CGGCCAGGAC GGCAAGAAGG ACTTCCTGTC TCTCACCCTG 
GTCAACGGCN NNNNNNNNNG AAGGTTCAAC ACAGGTTCGG GCACAGGCAC CTTGGTCAGC 
AAGGTCCAGG TTAAGCAGGG TCGCTGGCAC CAGCTGGTAG TGACAAGGAA CCGGCGCAAC 
GCCATGCTGA GCGTGGACAA TGAGACCCAC ATCGACGGCG CCAGCCCCCC CGGCACCGAC 
GGCCTCAACC TGGACACTCA CCTGTTCATC GGAGGGGTGC CCGAAGACAT GATGACAGAT 
GTGAAGGAGA GGACCTCCAT CGGTACGGGT CTGGTGGGCT GTGTGCGCAT GCTGGACGTC 
AACAACCTGG TGTACAACCT GCAGGAGAAG AGTGGCAACG TGCTGTACGG CACCGGCGTG 
GGCGAGTGCG GCAACAATCC GTGCCAGCCT AACCCTTGCA AGAATGGCGC CCCATGCCAG 
GTCAAGGAGG CCGAGATGTT CCACTGCAAG TGCATCAACG GCTACTCCGG TCCGACGTGC 
GCCGACACCC ACAACCCGTG CGAGCCCAAC AGATGCCACC CCTTGTCCCA GTGCCAGGTG 
CAGCCGGAGG GAGGGTACAA GTGCGAGTGT CCCATGGGTC GCGAGGGACG CCACTGCGAG 
AAAGTGTCGG AGAAGAAAGG GGCCTACATG CCGTTCTTTA GCGGAGACTC TTACCTGGAG 
CTGAAGGGAT TGCACACCTA CGGTCACGAT CTCCACCAAA AGGTCAGCAT GACAGTGGTG 
CTCATGGCCA ATGACTCCAA CGGCATGATC TTCTACAGCG GCCAGAAGAC GGACGGCAAA 
GGTGACTTCA TCTCCCTCTC CCTCAACGAC GGCATCCTGG AGTTCCGCTA TGACCTGGGC 
AAGGGCCCCG CTGTCATCAG GAGTAAAGAT AAGATCAAGA TGGGCGTGTG GAACACAGTG 
AACCTGGAGC GGGCCAATCG AAAGGGAGAG ATTAACATCA ACGGAAAGGA CCCAGTCAGA 
GGAGAGTCGC CGAAATCACG GAAGAACCAA CACACCGCTC TCAACCTGAA GGAGTCTCTG 
TTTGTGGGTG GTGCTCCTGA TTTCAGCCGG CTAGCAAGGG CCGCCTCGCT CAAAGATGGC 
TTCAAGGGGA CCATTCAGAA GATCACCCTG ATGGGCACGC CCATCCTCCG GCAGGAGAAC 
GCGCTGCACT CCAGTGACGT GGCCATGTTC GAGGGCCACT CCTGCAGCCG TGAGCCCTGC 
CACAACGGGG GTCGCTGCAA CCCCCTGCTG GAGAGCTATG AGTGTGTGTG CCTCCTCGGC 
TTCACAGGCA CACACTGTCA GAACACCATC TTCGAGAAGT CCGCCGGGGA AACGGAGTCC 
ATCGCCTTTG ACGGCCGGAC ATTCATTGAG TACCACAACG CCGTCACCAA GAGCCAACTG 
ACCAATGAGA TTCCGGATCC AGAGTCACTG GAGAACCCGT CAGAGCAAAG TGAGAAAGCC 
CTGCTGGTGA ACAAGTTTGA ACTGAGCATT AAGACCGAGG CCACTCAGGG CCTGGTGCTG 
TGGAGTGGTA AGGGCGTGGA GCGATCTGAC TACATCGCCC TGGCGGTGGT GGATGGCCGT 
GTGCAGATGA CCTACGACCT GGGCTCCAAG CCTGTGGTGC TGCGCTCGAC GGTCCGCATC 
AACACCAACC GCTGGATCCA CATCAAGGCC AGCAGAGCAC TTAGGGACGG CTCTCTCCAG 
GTGGGCAACG AGGCAGCGGT CACAGGGTCG TCGCCCCTGG CAGCCACTCA GCTGGACACA 
GACGGAGCCC TCTGGTTGGG TGGCTTGGAG GAGTTGGCTG TGGCCCGGAG ACTGCCTAAG 
GCCTACAGCA CAGGTTTTGT GGGCTGTGTA AAGGATGTCG CGGTGGACGG CGTGGAGCTC 
CACCTGGTGG AGGACGCCCT GAACAGCCCC AAAATATTAC ACTGTTCCGC CTCTGGCACA 
GCCGCCACGG CCAAAAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021471523.1
CDS	226..6486
Translation	MVSQRRPTHLYGWALMIVAVFCQRCHGSCPEKDLEKREEEANVVLTGTVEEIMNMDPVHNTYSCKVRVWRYLKGKTMVNGEVLLDGGNKVMIGGFGDPVICDNQVATGDTRIFFVNLAPEYMWPAHKNELMLNSSLMRITLRNLEEVEHCVDDKPVHFTPAQPPDGCRGMLCGFGAVCEREATDPSKAECVCKKMVCPSVVAPVCGSDSSTYSNECELEKAQCNTQRRIKVLRKGPCALKDPCSEVICSYGSTCIQSSDGLSAKCMCPLSCEGKPEQVMCGSDGQDYRNECELHKQACHTKKNIRLQRQGSCNPCKGLENNLNVACRVEPRTHQPLNIPRPESCPAVNEPLCASDGQTYESECHMKRTGMQKGIELKKNHPGRCKKQEECREECKFNGVCQVERLGMRCSCEPIQCDGTYKPLCGKDGHTYINDCERRRAECQAKAHIPVKQQGPCDLHTPSPCLGKACEFGAACVVKNNEPVCECHGACPQMPDLVCGSDGHSYGSQCEMKAMGCALQKEIHIQHRGPCDEACANCSFGAICDGQSGRCVCPQECVESHQPVCGSDGSTYDSECELHVRACTEQLDLRVLAQGECKTCGSAVCAWGARCVQNKCECPQCQGQAFSPVCGSDGFTYDNTCELGVASCVLKKKIEAAKPGSCDEECGSGGSGSGIESCEQDRCRTFGGSWDEDAEDDRCVCDFDCHRVPRNPVCGSNGKTYSNECEMKKARCEKQEHLLIQNQGPCTAISATSPTKLPAQEHCSLSVYGCCRDNKTAALGVGLAGCPSTCQCNPYGSYKGTCDPGSGQCSCKPGVGGQKCDRCDPSFWNFRGIVTENLSGCTPCNCDAVGSVRDDCEQMSGLCSCKTGVKGMKCNVCPDGSQMGMSGCDTGADAPTSCDELDCKFGALCDEVNGQAHCECPSPDCDEKNKTKVCGSDGVTYADQCQLRTIACRQDKHITVERFGQCTESIIEPAGRSTLYPPTSHAAATTPSTAHVRLTSPHYDASLSPWDDDQKNGLPASFVEALPPPVATESALTTNRPVTTPRHPPHSTTPHLPGHTSPGSSPTAFEDSGSGEPSGDDQVAEEEENEEEGSAFGVLEASGEEPVDPTAAATTIPTAEDRSSCDNTPFGCCPDGKTASSSPEGANCPPTMRFSGFLHLDQVEGQEVFYTPEMEDPKSELFGETARSIESALNELFRKSEVQKDFMSVRVRSLAPSNSILAFVEAHFDSETKYTVEDIEGALLKQLKAAKETAIMVKKPEEENIRFTNYGLSSLPYFTTTTTTTATATTPTASPSPFITSRPPGTTRRPYTSRRTTTAPSPVTTPASTTTITPLHRTRPVPHHTKAQRPCDSHPCRHGGTCEDDGADFTCICPAGRGGAVCEKLIKYFIPSFGGKSYLAFDTMKAYHTVRIAMEFRAAEMTGILLFNGQDGKKDFLSLTLVNGnullnullnullnullRFNTGSGTGTLVSKVQVKQGRWHQLVVTRNRRNAMLSVDNETHIDGASPPGTDGLNLDTHLFIGGVPEDMMTDVKERTSIGTGLVGCVRMLDVNNLVYNLQEKSGNVLYGTGVGECGNNPCQPNPCKNGAPCQVKEAEMFHCKCINGYSGPTCADTHNPCEPNRCHPLSQCQVQPEGGYKCECPMGREGRHCEKVSEKKGAYMPFFSGDSYLELKGLHTYGHDLHQKVSMTVVLMANDSNGMIFYSGQKTDGKGDFISLSLNDGILEFRYDLGKGPAVIRSKDKIKMGVWNTVNLERANRKGEININGKDPVRGESPKSRKNQHTALNLKESLFVGGAPDFSRLARAASLKDGFKGTIQKITLMGTPILRQENALHSSDVAMFEGHSCSREPCHNGGRCNPLLESYECVCLLGFTGTHCQNTIFEKSAGETESIAFDGRTFIEYHNAVTKSQLTNEIPDPESLENPSEQSEKALLVNKFELSIKTEATQGLVLWSGKGVERSDYIALAVVDGRVQMTYDLGSKPVVLRSTVRINTNRWIHIKASRALRDGSLQVGNEAAVTGSSPLAATQLDTDGALWLGGLEELAVARRLPKAYSTGFVGCVKDVAVDGVELHLVEDALNSPKILHCSASGTAATAKK

Target ORF information:

RefSeq Version	XM_021615848.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss agrin (agrn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021615848.1

ORF Insert Sequence:

ATGGTTTCAC AACGGAGACC AACTCACTTG TACGGGTGGG CATTGATGAT AGTCGCCGTT 
TTCTGTCAAC GATGTCACGG AAGTTGTCCG GAGAAAGACT TGGAGAAAAG GGAAGAGGAG 
GCGAACGTAG TTTTGACTGG TACCGTTGAA GAAATCATGA ACATGGACCC AGTGCATAAT 
ACATACTCGT GCAAGGTGAG GGTATGGCGG TACCTGAAAG GCAAGACCAT GGTCAACGGC 
GAGGTTCTTC TGGACGGCGG CAACAAGGTG ATGATCGGCG GCTTCGGGGA CCCTGTGATA 
TGCGACAACC AGGTTGCCAC TGGCGACACG CGCATTTTCT TTGTCAACCT GGCCCCGGAG 
TACATGTGGC CAGCACATAA GAACGAGCTG ATGCTCAACT CCAGCCTGAT GAGGATTACT 
CTGCGTAACC TGGAGGAGGT GGAGCACTGT GTGGACGACA AACCTGTTCA CTTCACGCCG 
GCCCAGCCTC CTGATGGTTG TAGAGGTATG CTGTGCGGCT TCGGGGCGGT GTGTGAGCGT 
GAAGCGACTG ACCCCTCTAA GGCGGAGTGT GTATGCAAGA AGATGGTTTG TCCGTCGGTT 
GTGGCGCCCG TGTGCGGCTC AGACTCCTCC ACCTACTCCA ACGAGTGCGA GCTGGAGAAG 
GCCCAGTGCA ACACGCAGCG ACGCATCAAG GTGCTGCGCA AGGGACCCTG TGCCCTGAAG 
GATCCATGCA GCGAGGTGAT CTGCAGTTAC GGCAGCACCT GCATCCAGTC ATCGGACGGC 
CTGTCGGCCA AGTGCATGTG CCCGCTGAGC TGCGAAGGCA AGCCCGAGCA GGTGATGTGT 
GGCAGCGATG GCCAGGACTA CCGCAACGAG TGTGAGCTCC ACAAGCAGGC CTGCCATACC 
AAGAAGAACA TCCGCCTTCA GCGCCAGGGA TCCTGCAACC CATGTAAAGG CCTCGAGAAC 
AACCTGAACG TTGCATGCCG TGTAGAACCG CGCACCCACC AGCCCCTGAA TATCCCCCGC 
CCGGAGTCCT GCCCTGCCGT CAATGAGCCA CTGTGTGCCA GCGATGGGCA GACGTACGAG 
AGTGAGTGCC ATATGAAGCG GACGGGCATG CAGAAGGGCA TTGAACTCAA GAAGAACCAC 
CCGGGCCGCT GCAAGAAACA AGAGGAGTGC AGGGAGGAGT GCAAGTTCAA CGGCGTGTGC 
CAGGTGGAGC GTCTGGGCAT GCGCTGCTCC TGCGAGCCCA TCCAGTGCGA TGGCACCTAC 
AAGCCGCTAT GCGGCAAGGA CGGACACACC TACATCAATG ACTGCGAGCG CCGGCGGGCT 
GAGTGCCAGG CCAAGGCCCA TATTCCCGTC AAGCAGCAAG GCCCGTGTGA TCTACACACT 
CCCAGCCCGT GCCTGGGGAA GGCCTGCGAG TTTGGGGCCG CGTGCGTGGT GAAGAACAAC 
GAGCCCGTGT GCGAGTGCCA TGGCGCCTGC CCCCAGATGC CCGACCTGGT GTGCGGCAGT 
GACGGCCACA GCTACGGCAG CCAGTGTGAG ATGAAGGCCA TGGGCTGCGC CCTGCAGAAG 
GAGATTCACA TCCAGCACCG AGGACCCTGC GACGAGGCGT GTGCCAACTG TTCGTTTGGG 
GCGATCTGCG ATGGGCAGTC TGGCCGCTGT GTGTGCCCGC AGGAGTGTGT GGAGTCCCAC 
CAACCGGTGT GCGGCAGCGA CGGCTCCACC TACGACAGCG AGTGTGAGCT CCACGTGCGT 
GCCTGTACCG AGCAGCTTGA CCTGCGCGTG CTCGCCCAGG GAGAATGCAA GACGTGCGGT 
AGCGCCGTGT GTGCCTGGGG GGCCCGCTGC GTCCAGAACA AGTGCGAGTG CCCGCAATGC 
CAGGGCCAGG CCTTCTCGCC CGTGTGCGGC AGCGACGGCT TCACCTACGA CAACACCTGC 
GAGCTGGGCG TGGCCTCCTG CGTCCTCAAA AAGAAGATTG AGGCGGCCAA GCCTGGCAGC 
TGCGACGAAG AGTGTGGTTC AGGAGGATCG GGGTCAGGTA TTGAGAGCTG CGAACAGGAC 
AGGTGTCGTA CGTTTGGAGG ATCCTGGGAC GAGGACGCAG AGGACGACCG CTGTGTCTGT 
GACTTCGACT GCCATCGCGT GCCTCGCAAC CCGGTGTGTG GCTCAAATGG GAAGACCTAC 
AGCAACGAGT GTGAAATGAA GAAGGCTAGG TGTGAAAAGC AGGAGCACCT GCTGATCCAG 
AATCAGGGAC CCTGTACAGC GATTTCGGCC ACCTCTCCGA CAAAACTGCC AGCGCAGGAA 
CACTGCAGTC TGTCAGTCTA TGGCTGTTGC CGTGACAACA AGACGGCGGC CCTTGGTGTG 
GGACTTGCCG GATGCCCCAG TACATGCCAG TGTAACCCGT ACGGCTCCTA CAAGGGGACT 
TGTGACCCGG GCAGCGGCCA GTGCTCCTGT AAGCCCGGCG TCGGGGGACA GAAGTGCGAC 
CGCTGCGATC CAAGCTTCTG GAACTTCCGC GGCATCGTCA CAGAGAACTT GAGCGGCTGC 
ACACCGTGTA ACTGCGACGC GGTGGGCTCG GTGAGGGACG ACTGCGAGCA AATGTCAGGC 
CTGTGCTCCT GCAAGACGGG AGTGAAGGGC ATGAAGTGCA ACGTGTGCCC CGACGGCAGC 
CAGATGGGTA TGAGCGGCTG CGACACAGGT GCCGATGCCC CAACTTCATG TGATGAGCTG 
GACTGCAAGT TTGGCGCGTT GTGTGACGAG GTCAATGGTC AAGCCCACTG CGAATGTCCC 
TCTCCCGACT GTGACGAGAA GAACAAGACC AAGGTGTGTG GCTCTGATGG GGTGACCTAC 
GCTGACCAGT GCCAGCTGAG GACCATTGCC TGTAGGCAGG ACAAGCACAT CACAGTGGAA 
CGCTTTGGCC AGTGTACAGA AAGCATCATA GAGCCGGCCG GCCGATCCAC TCTTTACCCC 
CCCACCTCCC ACGCCGCCGC CACCACCCCC TCCACCGCCC ACGTCAGGCT CACGTCGCCG 
CACTACGATG CATCACTCTC TCCCTGGGAC GATGATCAAA AGAACGGGCT CCCGGCCTCT 
TTCGTGGAAG CCTTGCCACC CCCCGTGGCC ACGGAGAGCG CGCTCACCAC CAACCGCCCC 
GTCACCACCC CCCGCCACCC ACCTCACTCC ACCACCCCCC ACCTGCCTGG CCACACCAGC 
CCTGGCTCCT CCCCTACCGC CTTTGAAGAC TCAGGGAGCG GCGAGCCCAG CGGAGACGAC 
CAGGTGGCGG AAGAGGAGGA GAATGAGGAA GAAGGTAGCG CCTTCGGAGT TCTGGAGGCG 
AGCGGAGAGG AGCCTGTTGA CCCCACAGCT GCGGCCACCA CCATCCCCAC GGCGGAAGAT 
CGCTCGTCGT GTGACAACAC TCCCTTCGGC TGCTGTCCCG ACGGCAAGAC GGCCTCCAGC 
AGCCCTGAGG GAGCCAATTG CCCCCCCACC ATGAGGTTCA GCGGCTTCCT GCACCTAGAC 
CAGGTGGAGG GCCAGGAGGT CTTCTACACC CCTGAGATGG AGGATCCCAA GTCTGAGCTG 
TTTGGGGAGA CGGCCAGGAG CATCGAGAGT GCTCTGAACG AGTTGTTCCG GAAGTCAGAG 
GTGCAGAAAG ACTTCATGAG TGTCCGCGTC CGTAGCCTGG CTCCCAGCAA CTCCATCTTG 
GCCTTCGTCG AGGCCCACTT TGACTCAGAG ACCAAGTACA CAGTGGAGGA CATCGAGGGA 
GCCCTGCTCA AGCAGCTGAA GGCAGCCAAG GAGACAGCCA TCATGGTGAA GAAGCCAGAG 
GAGGAGAACA TTCGCTTCAC CAACTACGGC CTCTCCTCCC TCCCCTACTT CACCACAACC 
ACCACTACAA CGGCCACAGC TACCACCCCC ACCGCCAGCC CCTCCCCCTT CATCACAAGC 
CGTCCCCCGG GCACCACCCG CCGCCCTTAC ACCAGCCGCC GGACCACCAC CGCCCCCTCG 
CCAGTGACCA CCCCGGCCTC CACTACTACC ATCACCCCCC TCCACAGGAC CAGGCCTGTG 
CCCCACCACA CCAAGGCCCA GCGGCCCTGC GACTCCCACC CCTGCCGCCA CGGGGGCACC 
TGCGAGGACG ACGGTGCCGA CTTCACCTGC ATCTGCCCCG CCGGGAGAGG GGGTGCCGTC 
TGTGAGAAAC TGATCAAGTA CTTCATTCCG TCGTTCGGGG GTAAGTCCTA CCTGGCCTTT 
GACACCATGA AGGCTTACCA CACGGTCCGC ATCGCCATGG AATTCAGGGC CGCCGAGATG 
ACGGGCATCC TCCTCTTCAA CGGCCAGGAC GGCAAGAAGG ACTTCCTGTC TCTCACCCTG 
GTCAACGGCN NNNNNNNNNG AAGGTTCAAC ACAGGTTCGG GCACAGGCAC CTTGGTCAGC 
AAGGTCCAGG TTAAGCAGGG TCGCTGGCAC CAGCTGGTAG TGACAAGGAA CCGGCGCAAC 
GCCATGCTGA GCGTGGACAA TGAGACCCAC ATCGACGGCG CCAGCCCCCC CGGCACCGAC 
GGCCTCAACC TGGACACTCA CCTGTTCATC GGAGGGGTGC CCGAAGACAT GATGACAGAT 
GTGAAGGAGA GGACCTCCAT CGGTACGGGT CTGGTGGGCT GTGTGCGCAT GCTGGACGTC 
AACAACCTGG TGTACAACCT GCAGGAGAAG AGTGGCAACG TGCTGTACGG CACCGGCGTG 
GGCGAGTGCG GCAACAATCC GTGCCAGCCT AACCCTTGCA AGAATGGCGC CCCATGCCAG 
GTCAAGGAGG CCGAGATGTT CCACTGCAAG TGCATCAACG GCTACTCCGG TCCGACGTGC 
GCCGACACCC ACAACCCGTG CGAGCCCAAC AGATGCCACC CCTTGTCCCA GTGCCAGGTG 
CAGCCGGAGG GAGGGTACAA GTGCGAGTGT CCCATGGGTC GCGAGGGACG CCACTGCGAG 
AAAGTGTCGG AGAAGAAAGG GGCCTACATG CCGTTCTTTA GCGGAGACTC TTACCTGGAG 
CTGAAGGGAT TGCACACCTA CGGTCACGAT CTCCACCAAA AGGTCAGCAT GACAGTGGTG 
CTCATGGCCA ATGACTCCAA CGGCATGATC TTCTACAGCG GCCAGAAGAC GGACGGCAAA 
GGTGACTTCA TCTCCCTCTC CCTCAACGAC GGCATCCTGG AGTTCCGCTA TGACCTGGGC 
AAGGGCCCCG CTGTCATCAG GAGTAAAGAT AAGATCAAGA TGGGCGTGTG GAACACAGTG 
AACCTGGAGC GGGCCAATCG AAAGGGAGAG ATTAACATCA ACGGAAAGGA CCCAGTCAGA 
GGAGAGTCGC CGAAATCACG GAAGAACCAA CACACCGCTC TCAACCTGAA GGAGTCTCTG 
TTTGTGGGTG GTGCTCCTGA TTTCAGCCGG CTAGCAAGGG CCGCCTCGCT CAAAGATGGC 
TTCAAGGGGA CCATTCAGAA GATCACCCTG ATGGGCACGC CCATCCTCCG GCAGGAGAAC 
GCGCTGCACT CCAGTGACGT GGCCATGTTC GAGGGCCACT CCTGCAGCCG TGAGCCCTGC 
CACAACGGGG GTCGCTGCAA CCCCCTGCTG GAGAGCTATG AGTGTGTGTG CCTCCTCGGC 
TTCACAGGCA CACACTGTCA GAACACCATC TTCGAGAAGT CCGCCGGGGA AACGGAGTCC 
ATCGCCTTTG ACGGCCGGAC ATTCATTGAG TACCACAACG CCGTCACCAA GAGCCAACTG 
ACCAATGAGA TTCCGGATCC AGAGTCACTG GAGAACCCGT CAGAGCAAAG TGAGAAAGCC 
CTGCTGGTGA ACAAGTTTGA ACTGAGCATT AAGACCGAGG CCACTCAGGG CCTGGTGCTG 
TGGAGTGGTA AGGGCGTGGA GCGATCTGAC TACATCGCCC TGGCGGTGGT GGATGGCCGT 
GTGCAGATGA CCTACGACCT GGGCTCCAAG CCTGTGGTGC TGCGCTCGAC GGTCCGCATC 
AACACCAACC GCTGGATCCA CATCAAGGCC AGCAGAGCAC TTAGGGACGG CTCTCTCCAG 
GTGGGCAACG AGGCAGCGGT CACAGGGTCG TCGCCCCTGG CAGCCACTCA GCTGGACACA 
GACGGAGCCC TCTGGTTGGG TGGCTTGGAG GAGTTGGCTG TGGCCCGGAG ACTGCCTAAG 
GCCTACAGCA CAGGTTTTGT GGGCTGTGTA AAGGATGTCG CGGTGGACGG CGTGGAGCTC 
CACCTGGTGG AGGACGCCCT GAACAGCCCC AAAATATTAC ACTGTTCCGC CTCTGGCACA 
GCCGCCACGG CCAAAAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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agrn cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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