LOC110502571 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110502571
Entrez Gene ID	110502571
Full Name	spectrin beta chain, non-erythrocytic 1-like
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021580713.1	XP_021436388.1	spectrin beta chain, non-erythrocytic 1-like isoform X2
XM_021580712.1	XP_021436387.1	spectrin beta chain, non-erythrocytic 1-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC110502571 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110502571 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf29775	XM_021580712.1 Latest version!	Oncorhynchus mykiss spectrin beta chain, non-erythrocytic 1-like (LOC110502571), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOf29776	XM_021580713.1 Latest version!	Oncorhynchus mykiss spectrin beta chain, non-erythrocytic 1-like (LOC110502571), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110502571 ( XM_021580712.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021436387.1 Oncorhynchus mykiss spectrin beta chain, non-erythrocytic 1-like (LOC110502571), transcript variant X1, mRNA.

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CloneID	OOf29775
Clone ID Related Accession (Same CDS sequence)	XM_021580712.1
Accession Version	XM_021580712.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	spectrin beta chain, non-erythrocytic 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035099.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTAC AGAGGGCAAC CAGCATGCCT GGCCCCTTGT CCCCAGGGCC TGTCTTTCCA 
GGGTCCCACT CCCCAGGGCC CCTGTCCCCT TCCTCTGGGC CCGGCCCCTC TTTTGCCTCC 
AGCCCCGGCC CTGATCCGGG CTACTCTGCT AAACAGGCTG CCTTCAACTA CAACCAACTC 
GAGGGCCGAT TTAAGCAGCT GCAAGATGAG CGTGAAGCGG TGCAGAAGAA GACCTTCACC 
AAGTGGGTCA ACTCCCACCT AGCCAGAGTG TCCTGCAGGA TCACTGACCT CTATATGGAC 
CTGAGAGATG GACGCATGCT CATCAAACTA CTGGAGGTTC TCTCTGGGGA GAAACTGCCC 
AAGCCCACTA AGGGGCGCAT GCGTATCCAC TGCCTGGAGA ACGTGGACAA GGCCCTGCAG 
TTCCTCAAGG AGCAGAGGGT CCACCTAGAA AACATGGGCT CTCATGACAT CGTTGATGGC 
AACCACCGCC TCACCCTTGG CCTCATCTGG ACCATCATCC TCCGATTCCA GATCCAGGAC 
ATCAGAGTGG AGACGGAGGA TAACAAGGAG AAGAAGTCGG CTAAAGATGC TCTGCTGCTC 
TGGTGCCAGA TGAAGACCGC AGGATATCCC AACGTAAACA TTCACAACTT CAGCACCAGC 
TGGAGGGATG GCATGGCTTT CAACGCCATC CTCCACAAAC ACAGACCTGA CCTGATAGAC 
TTTGACAAGT TGAAAAAGTC AAATGCCCAC CACAACCTGC AGAATGCCTT CAACCTGGCA 
GAGCAACACC TGGGCCTCCA CAAGCTACTG GACGTAGAGG ATATCAGCGT GGACCACCCT 
GATGAGAAGT CCATCATCAC CTATGTTGTG ACCTACTACC ACTACTTCTC CAAGATGAAG 
GCCCTGAAGG TGGAGGGCAA ACGGATTGGG AAGGTGCTGG ACAATGCCAT TGAGACCGAT 
AACATGATTC AGAAATATGA GAGTCTGGCC TCTGACCTCC TGGAATGGAT TGAACAGACC 
ATCATCATCC TCAACAACCG CAGGTTTGCC AACTCCCTAG TGGGGGTGCA GCAACAGCTC 
CAGGCCTTCA ACACCTACCG TACCGTCGAA AAGCCACCCA AGTTCACTGA GAAAGGCAAC 
CTGGAGGTTC TCCTCTTCAC CATCCAGAGC AAGATGAGGG CCAACAATCA GAAGGTCTAC 
ATCCCGCGGG ACGGAAAACT CATCTCAGAC ATCAACAAGG CCTGGGAGAG GCTGGAGAAG 
TCAGAGCATG AGAGGGAGCT GGCTCTAAGG ACTGAGCTGA TTCGTCAGGA GAAACTGGAG 
CAGCTGGCCC GCCGCTTTGA CCGCAAAGCC GCCATGAGAG AGACCTGGCT GAGTGAGAAC 
CAACGGCTCG TCTCACAGGA CAACTTTGGC TTCGACCTGC AGGCGGTGGA GGCCGCCACC 
AAGAAGCACG AGGCCATCGA GACGGACATC GGCGCCTACG AGGAGCGTGT GCAAGCAGTG 
CTCTCCGTGG CCAAGGAGCT GGAGGCAGAG AACTACCATG ACATCAAGCG CGTGACAGCG 
AGGAAGGACA ACGTGCTGCG TCTGTGGGAG TACCTGTTGG AGCTGCTGAA GGCCCGGCGC 
ATCCGGCTGG AGATGAACCT GGGCCTGCAG AGGGTCTTCC AGGAGATGCT TTACATCATG 
GACTGGATGG ATGAGATGAA GATGCTGCTG CTGTCTCAGG ACTATGGGAA ACACCTGCTG 
GGAGTGGAGG ACCTGCTGCA GAAGCACACT CTGGTGGAGG CTGATATTGC CATCCAGGCC 
GACCGCGTCA AAGCCGTCAA CAACAACGCT CAGAAGTTCG CCATTGATGC AGAGGGCTAC 
AAGCCCTGTG ACCCCCAGGT GATCCGGGAC CGAGTGGCCC ACATGGAGTT CTGTTACGCA 
GAGCTGAGCC AGCTGGCCGC CGATCGCTGC GCCCGCCTGG AGGAGTCCCG CCGCCTTTGG 
AAGTTCTTCT GGGAGATGGC CGAGGAGGAG GGCTGGATCC GTGAGAAAGA GCAGATCCTG 
TCCTCGGAGG ACTGTGGTAA GGATCTGACG GGCACCGTGC GTCTGCTGAG CCAGCACCGC 
GCCTTCGAGG ATGAGATGAT CGGCCGCGCC GGCCACCTGC AACAGACCAT CAAGCAGGGA 
GAGGCGCTGG TGGCCGCCGA CCACTTCGGA GCCGACAAGA TCCGTGAGCG CATCCAAGAC 
ATCCAGGAGC AGTGGGCGGC CTTGGAGAGG CTATCTGCCG TTAGGAAGGC CCGGCTGGAG 
GAGGCCTGCA ACCTGCACCA GTTCCAGGCT GATGCCGACG ACATCGACGC CTGGATGCTG 
GATGTTCTCC GCATTGTGTC CAGTGCCGAC GTGGGCCACG ACGAGTTCTC CACCCAGGCC 
CTGGTCAAGA AGCACAAGGA TGTGGCCGAG GAGATTGCCA GCTACCGACC TGTCATCGAC 
GCCCTGCACG AGCAGTCCAA AACCCTGCCC GGGGAACAGA CTGGCTCAGA GGAGGTGCAG 
GGGCGCCTGT CGGGCATCGA GGAGCGCTAT AAGGAGGTGG CTGAGCTGAC CCGGCTGAGG 
AAGCAGGCCC TACAGGATGC CCTGGCCCTC TACAAGATGT TCAGCGAGGC GGATGCCTGT 
GAGGTGTGGA TCGATGAGAA GGAGCAGTGG CTCAACAGTA TGGAGATACC AGAGAAACTG 
GAGGATCTGG AAGTCGTTCA GCACAGGTTT GAAAGTCTGG AGCCAGAGAT GAACAACCAA 
GCCTCACGCG TTGCCGTGGT GAACCAGATT GCTAGGCAAC TCATCCACAG TGGTCACCCC 
AGTGAAAAGG AGATCAAAGC CCAACAGGAC AAACTCAACA CCAGATGGAG TCAGTTCCGT 
GACCTAGTTG ACCTGAAGAA AGACTCCCTC AACTCAGCTC TGGGTGTACA GAACTACCAC 
CTGGAGTGTA ACGAGACCAA GTCGTGGATC CGCGAGAAGA CCAAGGTGAT CGAGTCCACC 
CAGGAGCTGG GTAATGACCT GGCCGGGGTC ATGGCCCTGC AGCGCAAGCT CACCGGCATG 
GAGCGTGACC TGACCGCCAT CGAGGACAAG CTAGGAGAAC TGGGGAAGGA GGGGGAGCAC 
CTTGCAGCCG AGCACCCTGA CCAGGCCCAG GGCATCATGG GTCGCCTGGG GGAGATCACA 
GGAGTGTGGG ACGAGATGAA GGGCACCCTG AAGAACCGCG AGGAGTCCCT GGGCGAGGCC 
AGCAAGCTGC AGCAGTTCCT CCGGGAGCTG GATGACTTCC AGTCATGGCT TTCCAAGACC 
CAGACGGCCA TCGCTTCAGA AGACATGCCC AACACACTGA CGGAGGCCGA AAAGCTGCTG 
GCGCAGCATG AGGGCATCAA GAACGAGATC CGCAACTATG AAGTGGACTA CCAGAAGATG 
AGGGACATGG GGGAAATGGT GACCCAGGGC CAGACGGACG CCCAGTACAT GTTCCTGAGG 
CAGAGGCTGC AGGCCCTGGA CACAGGCTGG AACGAGCTGC ACAAGATGTG GGAGAACCGT 
CAGAACCTGT TGTCCCAGTC CCATGCCCAC CAGCTGTTCC TCAGGGATAC CAAGCAGGCT 
GAAGCCTTCC TCAACAACCA GGAGTACGTG CTGGCCCATA CTGAGATGCC CACAACTCTG 
GAGGGGGCAG AGGGAGCCAT TAAGAAGCAG GAGGACTTCA TGACCACCAT GGACGCCAAC 
GAGGAGAAGA TCAATGCCGT GGTGGACACC GGACGCAGAC TGGTCAGTGA CAAAAACATC 
AATGCTGACC GCATCCAGGA GAAAGTGGAC TCTATTGATG AGAGACATCA GAAGAACCGC 
TTGGCGGCCA GTGAGCTTCT CTGTAAGCTG AAGGATAACA GAGACCTGCA GAAGTTCCTT 
CAGAACTGCC AAGAGCTGTC CCTGTGGATC AATGAGAAGA TGCTCACGGC TCAGGACATG 
TCGTACGACG AAGCCAGGAA CCTCCACAGC AAATGGCTCA AACACCAGGC CTTCATGGCC 
GAGCTTCAGT CCAACAAGGA GTGGCTGGAC GAGATCGAGA AGGACGGGAT GCAGCTGATA 
GCTGAGAAGC CGGAGACCGA GGCCATGGTG AAGGAAAAGC TGGCAGCGCT CCATAAGATG 
TGGGATGACC TTGAGTCCAC CACCCAGACT AAGGCCAAGT GTCTGTTTGA CGCCAACAAG 
GCTGAGCTGT TCACCCAGAG TTGTGCCGAC CTGGACAAGT GGCTGGTGGG AGTGGATGGA 
CAAATCCAAT CAGATGACTA CGGCAAAGAT CTGACCAGCG TCAACATCCT GCTCAAGAAA 
CAACAGATGC TGGAGAGCCA GGTGGAGGTG CGTCAGAAGG AGGTGGAGGA GCTGCAGAGC 
CAGGCCCAGG TCCTCAGCCA GGAGGGGAAG GACACAGATG AGGTGGACGG CCAGCGCCGC 
ATCGTGGAGA AGAAGTTCCA GCAGCTGCTA GACCCACTGA AGAAGAGGAA GGGAAACCTC 
ATGGCCTCCC GCGAGATCCA CCAGTTCAAC AGAGACGTTG AGGACGAGAT CCTCTGGGTT 
GAAGAGAGAA TGCCACTGGC AACATCTACA GACCACGGAA ACAACCTGCA AACAGTCCAG 
TTACTCATCA AGAAAAACCA GACCCTGCAA AAGGAGATCC AGGGCCACCA GCCGCGCTGT 
GACGACATCT TTGAGCGGAG TCAGCAGGTG CTGAAGGATG ACGACAACAG CCCCAGCGGC 
GAGGGGATCC GCCAGCGGCT GGCCGACCTG CGGCGCCTTT GGGCCCAGAT CCTGGAGGAG 
ACAGCCAAAC GCCATGGACG CCTGGAGGAG GCCCACAAGG CCCAGCAGTA CTACTTTGAT 
GCTGCCGAGG CTGAGGCCTG GATGAGCGAG CAGGAGCTCT ATATGATGTC AGAGGAGAAA 
GCCAAGGATG AGCAGAGCTC CGTGGCCATG CTGAAGAAGC ACCAGATCCT GGAGCAGGCT 
GTGGAGGACT ATGCTGAGAC TGTCCACCAG CTCTCCAAGA CCAGCAGGGG CCTGACGGCC 
GCCGGACACC CAGAAAGTGA ACGGATCGGC ATGCGTCAGT CCCAGGTGGA TAAGCTGTAC 
GCGGGGCTCA AGGACCTGTC GGAGGAGAGG AGGGGGAAGT TGGACGAGAG GTTCCGTCTT 
TTTCAGCTCA ACAGGGAGGT GGATGATCTG GAGCAGTGGA TCGCTGAAAG GGAGGTGGTG 
GCTGGGTCAC ATGAGCTGGG ACAGGACTAT GAACATGTCA CGATGCTTCA GGAGCGTTTC 
CGTGAGTTTG CTCGCGACAC AGGCAACATT GGTCAAGAGC GTGTGGACAC TGTCAACCAG 
CTGGCGGACG AACTCATCAA CTCGGGCCAC GCCGACGCGG CTACCATCGC CGAGTGGAAG 
GACGGGCTGA ATGAGGCGTG GGCCGACCTG CTGGAGCTGA TTGACACACG CACGCAGATC 
CTGGCGGCGT CCTTCGAGCT GCACAAGTTC TACCACGACG CCAAGGAGAT CCTCAACCGC 
ATCCTGGACA AACACAAGAA GCTGCCTGAG GAGCTGGGCC GGGACCAGAA CACAGTGGAG 
ACCCTGCAGA GGATGCACAC CACCTTCGAA CACGACATCC AAGCCCTGGG GACACAGGTG 
AGGATGTTGC AGGAGGATGC TGTGCGCCTG CAGTCTGCCT ATGCCGGAGA CAAGGCTGAT 
GACATCCAAA AGAGAGAGGG TGAGGTTTTG GAGGCCTGGA GAACACTGCT GGAGGCCTGT 
GACGGCCGCA GGGTCAGACT GCTCGACACC GGCGACAAGT TCCGTTTCTT CAGCATGGTC 
CGAGACCTCA TGCTCTGGAT GGAGGACGTC ATCCGCCTCA TCGAGGCTCA GGAGAAGCCC 
AGGGACGTGT CGTCTGTGGA GCTCCTGATG AACAACCACC AGGGCATCAA GGCTGAGATC 
GACGCCCGTA ACGACAGCTT TACCAACTGC ATCGAGCTGG GCAAGTCCCT GTTGGCACGA 
AAACACTACG CATTAGAGGA GATCAAAGAG AAGTTACTAC AGTTGACGGA CAAGAGGAAA 
GACATGATTG ACAAGTGGGA GGACAGATGG GAGTGGCTTA GATTGGTTCT GGAGGTGCAC 
CAGTTTGGAA GGGACGCGGG TGTGGCCGAG TCGTGGCTGC TGGGACAGGA GCCGTACCTG 
TCCAGCAGGG AGATGGGTCA GAACGTGGAT GAAGTGGAGA AACTCATCAA GAGACACGAG 
GCCTTTGAGA AGTCAGCTGC CACCTGGGAA GAGCGGTTCT CTGCGCTGGA ACGGCTGACT 
ACGATGGAAT TACTGGAAGT GAGAAGACAG CAAGAGGAGG AGGAGAGGAG GAGAAATCCC 
CCACCTGCAG AGACCCAGAC GGACACTGCA TCGCCACAGC AGAAGGAGGG TGAGCAAGTG 
TCTCTGAATG GACTGCCATC GGACCAGGAC TCCCCTCGGG TTAGTTACCG CTCCCCAGCC 
TACCAAAAGT ACTCCAGCTT TCAAACCCGC AGGACTGGGA CTGGGCGAGA AAGGCGGGCG 
GGCTGGACCG TGACCCCCCA ATGCCCCCCT TGCCCTTTGA ACCCCCTGCC CCCACCACAG 
GGAGAGGACT GTACACCCCT CTACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021436387.1
CDS	370..6999
Translation	MELQRATSMPGPLSPGPVFPGSHSPGPLSPSSGPGPSFASSPGPDPGYSAKQAAFNYNQLEGRFKQLQDEREAVQKKTFTKWVNSHLARVSCRITDLYMDLRDGRMLIKLLEVLSGEKLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDIRVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFSTSWRDGMAFNAILHKHRPDLIDFDKLKKSNAHHNLQNAFNLAEQHLGLHKLLDVEDISVDHPDEKSIITYVVTYYHYFSKMKALKVEGKRIGKVLDNAIETDNMIQKYESLASDLLEWIEQTIIILNNRRFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYIPRDGKLISDINKAWERLEKSEHERELALRTELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLQAVEAATKKHEAIETDIGAYEERVQAVLSVAKELEAENYHDIKRVTARKDNVLRLWEYLLELLKARRIRLEMNLGLQRVFQEMLYIMDWMDEMKMLLLSQDYGKHLLGVEDLLQKHTLVEADIAIQADRVKAVNNNAQKFAIDAEGYKPCDPQVIRDRVAHMEFCYAELSQLAADRCARLEESRRLWKFFWEMAEEEGWIREKEQILSSEDCGKDLTGTVRLLSQHRAFEDEMIGRAGHLQQTIKQGEALVAADHFGADKIRERIQDIQEQWAALERLSAVRKARLEEACNLHQFQADADDIDAWMLDVLRIVSSADVGHDEFSTQALVKKHKDVAEEIASYRPVIDALHEQSKTLPGEQTGSEEVQGRLSGIEERYKEVAELTRLRKQALQDALALYKMFSEADACEVWIDEKEQWLNSMEIPEKLEDLEVVQHRFESLEPEMNNQASRVAVVNQIARQLIHSGHPSEKEIKAQQDKLNTRWSQFRDLVDLKKDSLNSALGVQNYHLECNETKSWIREKTKVIESTQELGNDLAGVMALQRKLTGMERDLTAIEDKLGELGKEGEHLAAEHPDQAQGIMGRLGEITGVWDEMKGTLKNREESLGEASKLQQFLRELDDFQSWLSKTQTAIASEDMPNTLTEAEKLLAQHEGIKNEIRNYEVDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAHQLFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEGAIKKQEDFMTTMDANEEKINAVVDTGRRLVSDKNINADRIQEKVDSIDERHQKNRLAASELLCKLKDNRDLQKFLQNCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELQSNKEWLDEIEKDGMQLIAEKPETEAMVKEKLAALHKMWDDLESTTQTKAKCLFDANKAELFTQSCADLDKWLVGVDGQIQSDDYGKDLTSVNILLKKQQMLESQVEVRQKEVEELQSQAQVLSQEGKDTDEVDGQRRIVEKKFQQLLDPLKKRKGNLMASREIHQFNRDVEDEILWVEERMPLATSTDHGNNLQTVQLLIKKNQTLQKEIQGHQPRCDDIFERSQQVLKDDDNSPSGEGIRQRLADLRRLWAQILEETAKRHGRLEEAHKAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSSVAMLKKHQILEQAVEDYAETVHQLSKTSRGLTAAGHPESERIGMRQSQVDKLYAGLKDLSEERRGKLDERFRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNQLADELINSGHADAATIAEWKDGLNEAWADLLELIDTRTQILAASFELHKFYHDAKEILNRILDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRMLQEDAVRLQSAYAGDKADDIQKREGEVLEAWRTLLEACDGRRVRLLDTGDKFRFFSMVRDLMLWMEDVIRLIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTNCIELGKSLLARKHYALEEIKEKLLQLTDKRKDMIDKWEDRWEWLRLVLEVHQFGRDAGVAESWLLGQEPYLSSREMGQNVDEVEKLIKRHEAFEKSAATWEERFSALERLTTMELLEVRRQQEEEERRRNPPPAETQTDTASPQQKEGEQVSLNGLPSDQDSPRVSYRSPAYQKYSSFQTRRTGTGRERRAGWTVTPQCPPCPLNPLPPPQGEDCTPLYP

Target ORF information:

RefSeq Version	XM_021580712.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss spectrin beta chain, non-erythrocytic 1-like (LOC110502571), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021580712.1

ORF Insert Sequence:

ATGGAGCTAC AGAGGGCAAC CAGCATGCCT GGCCCCTTGT CCCCAGGGCC TGTCTTTCCA 
GGGTCCCACT CCCCAGGGCC CCTGTCCCCT TCCTCTGGGC CCGGCCCCTC TTTTGCCTCC 
AGCCCCGGCC CTGATCCGGG CTACTCTGCT AAACAGGCTG CCTTCAACTA CAACCAACTC 
GAGGGCCGAT TTAAGCAGCT GCAAGATGAG CGTGAAGCGG TGCAGAAGAA GACCTTCACC 
AAGTGGGTCA ACTCCCACCT AGCCAGAGTG TCCTGCAGGA TCACTGACCT CTATATGGAC 
CTGAGAGATG GACGCATGCT CATCAAACTA CTGGAGGTTC TCTCTGGGGA GAAACTGCCC 
AAGCCCACTA AGGGGCGCAT GCGTATCCAC TGCCTGGAGA ACGTGGACAA GGCCCTGCAG 
TTCCTCAAGG AGCAGAGGGT CCACCTAGAA AACATGGGCT CTCATGACAT CGTTGATGGC 
AACCACCGCC TCACCCTTGG CCTCATCTGG ACCATCATCC TCCGATTCCA GATCCAGGAC 
ATCAGAGTGG AGACGGAGGA TAACAAGGAG AAGAAGTCGG CTAAAGATGC TCTGCTGCTC 
TGGTGCCAGA TGAAGACCGC AGGATATCCC AACGTAAACA TTCACAACTT CAGCACCAGC 
TGGAGGGATG GCATGGCTTT CAACGCCATC CTCCACAAAC ACAGACCTGA CCTGATAGAC 
TTTGACAAGT TGAAAAAGTC AAATGCCCAC CACAACCTGC AGAATGCCTT CAACCTGGCA 
GAGCAACACC TGGGCCTCCA CAAGCTACTG GACGTAGAGG ATATCAGCGT GGACCACCCT 
GATGAGAAGT CCATCATCAC CTATGTTGTG ACCTACTACC ACTACTTCTC CAAGATGAAG 
GCCCTGAAGG TGGAGGGCAA ACGGATTGGG AAGGTGCTGG ACAATGCCAT TGAGACCGAT 
AACATGATTC AGAAATATGA GAGTCTGGCC TCTGACCTCC TGGAATGGAT TGAACAGACC 
ATCATCATCC TCAACAACCG CAGGTTTGCC AACTCCCTAG TGGGGGTGCA GCAACAGCTC 
CAGGCCTTCA ACACCTACCG TACCGTCGAA AAGCCACCCA AGTTCACTGA GAAAGGCAAC 
CTGGAGGTTC TCCTCTTCAC CATCCAGAGC AAGATGAGGG CCAACAATCA GAAGGTCTAC 
ATCCCGCGGG ACGGAAAACT CATCTCAGAC ATCAACAAGG CCTGGGAGAG GCTGGAGAAG 
TCAGAGCATG AGAGGGAGCT GGCTCTAAGG ACTGAGCTGA TTCGTCAGGA GAAACTGGAG 
CAGCTGGCCC GCCGCTTTGA CCGCAAAGCC GCCATGAGAG AGACCTGGCT GAGTGAGAAC 
CAACGGCTCG TCTCACAGGA CAACTTTGGC TTCGACCTGC AGGCGGTGGA GGCCGCCACC 
AAGAAGCACG AGGCCATCGA GACGGACATC GGCGCCTACG AGGAGCGTGT GCAAGCAGTG 
CTCTCCGTGG CCAAGGAGCT GGAGGCAGAG AACTACCATG ACATCAAGCG CGTGACAGCG 
AGGAAGGACA ACGTGCTGCG TCTGTGGGAG TACCTGTTGG AGCTGCTGAA GGCCCGGCGC 
ATCCGGCTGG AGATGAACCT GGGCCTGCAG AGGGTCTTCC AGGAGATGCT TTACATCATG 
GACTGGATGG ATGAGATGAA GATGCTGCTG CTGTCTCAGG ACTATGGGAA ACACCTGCTG 
GGAGTGGAGG ACCTGCTGCA GAAGCACACT CTGGTGGAGG CTGATATTGC CATCCAGGCC 
GACCGCGTCA AAGCCGTCAA CAACAACGCT CAGAAGTTCG CCATTGATGC AGAGGGCTAC 
AAGCCCTGTG ACCCCCAGGT GATCCGGGAC CGAGTGGCCC ACATGGAGTT CTGTTACGCA 
GAGCTGAGCC AGCTGGCCGC CGATCGCTGC GCCCGCCTGG AGGAGTCCCG CCGCCTTTGG 
AAGTTCTTCT GGGAGATGGC CGAGGAGGAG GGCTGGATCC GTGAGAAAGA GCAGATCCTG 
TCCTCGGAGG ACTGTGGTAA GGATCTGACG GGCACCGTGC GTCTGCTGAG CCAGCACCGC 
GCCTTCGAGG ATGAGATGAT CGGCCGCGCC GGCCACCTGC AACAGACCAT CAAGCAGGGA 
GAGGCGCTGG TGGCCGCCGA CCACTTCGGA GCCGACAAGA TCCGTGAGCG CATCCAAGAC 
ATCCAGGAGC AGTGGGCGGC CTTGGAGAGG CTATCTGCCG TTAGGAAGGC CCGGCTGGAG 
GAGGCCTGCA ACCTGCACCA GTTCCAGGCT GATGCCGACG ACATCGACGC CTGGATGCTG 
GATGTTCTCC GCATTGTGTC CAGTGCCGAC GTGGGCCACG ACGAGTTCTC CACCCAGGCC 
CTGGTCAAGA AGCACAAGGA TGTGGCCGAG GAGATTGCCA GCTACCGACC TGTCATCGAC 
GCCCTGCACG AGCAGTCCAA AACCCTGCCC GGGGAACAGA CTGGCTCAGA GGAGGTGCAG 
GGGCGCCTGT CGGGCATCGA GGAGCGCTAT AAGGAGGTGG CTGAGCTGAC CCGGCTGAGG 
AAGCAGGCCC TACAGGATGC CCTGGCCCTC TACAAGATGT TCAGCGAGGC GGATGCCTGT 
GAGGTGTGGA TCGATGAGAA GGAGCAGTGG CTCAACAGTA TGGAGATACC AGAGAAACTG 
GAGGATCTGG AAGTCGTTCA GCACAGGTTT GAAAGTCTGG AGCCAGAGAT GAACAACCAA 
GCCTCACGCG TTGCCGTGGT GAACCAGATT GCTAGGCAAC TCATCCACAG TGGTCACCCC 
AGTGAAAAGG AGATCAAAGC CCAACAGGAC AAACTCAACA CCAGATGGAG TCAGTTCCGT 
GACCTAGTTG ACCTGAAGAA AGACTCCCTC AACTCAGCTC TGGGTGTACA GAACTACCAC 
CTGGAGTGTA ACGAGACCAA GTCGTGGATC CGCGAGAAGA CCAAGGTGAT CGAGTCCACC 
CAGGAGCTGG GTAATGACCT GGCCGGGGTC ATGGCCCTGC AGCGCAAGCT CACCGGCATG 
GAGCGTGACC TGACCGCCAT CGAGGACAAG CTAGGAGAAC TGGGGAAGGA GGGGGAGCAC 
CTTGCAGCCG AGCACCCTGA CCAGGCCCAG GGCATCATGG GTCGCCTGGG GGAGATCACA 
GGAGTGTGGG ACGAGATGAA GGGCACCCTG AAGAACCGCG AGGAGTCCCT GGGCGAGGCC 
AGCAAGCTGC AGCAGTTCCT CCGGGAGCTG GATGACTTCC AGTCATGGCT TTCCAAGACC 
CAGACGGCCA TCGCTTCAGA AGACATGCCC AACACACTGA CGGAGGCCGA AAAGCTGCTG 
GCGCAGCATG AGGGCATCAA GAACGAGATC CGCAACTATG AAGTGGACTA CCAGAAGATG 
AGGGACATGG GGGAAATGGT GACCCAGGGC CAGACGGACG CCCAGTACAT GTTCCTGAGG 
CAGAGGCTGC AGGCCCTGGA CACAGGCTGG AACGAGCTGC ACAAGATGTG GGAGAACCGT 
CAGAACCTGT TGTCCCAGTC CCATGCCCAC CAGCTGTTCC TCAGGGATAC CAAGCAGGCT 
GAAGCCTTCC TCAACAACCA GGAGTACGTG CTGGCCCATA CTGAGATGCC CACAACTCTG 
GAGGGGGCAG AGGGAGCCAT TAAGAAGCAG GAGGACTTCA TGACCACCAT GGACGCCAAC 
GAGGAGAAGA TCAATGCCGT GGTGGACACC GGACGCAGAC TGGTCAGTGA CAAAAACATC 
AATGCTGACC GCATCCAGGA GAAAGTGGAC TCTATTGATG AGAGACATCA GAAGAACCGC 
TTGGCGGCCA GTGAGCTTCT CTGTAAGCTG AAGGATAACA GAGACCTGCA GAAGTTCCTT 
CAGAACTGCC AAGAGCTGTC CCTGTGGATC AATGAGAAGA TGCTCACGGC TCAGGACATG 
TCGTACGACG AAGCCAGGAA CCTCCACAGC AAATGGCTCA AACACCAGGC CTTCATGGCC 
GAGCTTCAGT CCAACAAGGA GTGGCTGGAC GAGATCGAGA AGGACGGGAT GCAGCTGATA 
GCTGAGAAGC CGGAGACCGA GGCCATGGTG AAGGAAAAGC TGGCAGCGCT CCATAAGATG 
TGGGATGACC TTGAGTCCAC CACCCAGACT AAGGCCAAGT GTCTGTTTGA CGCCAACAAG 
GCTGAGCTGT TCACCCAGAG TTGTGCCGAC CTGGACAAGT GGCTGGTGGG AGTGGATGGA 
CAAATCCAAT CAGATGACTA CGGCAAAGAT CTGACCAGCG TCAACATCCT GCTCAAGAAA 
CAACAGATGC TGGAGAGCCA GGTGGAGGTG CGTCAGAAGG AGGTGGAGGA GCTGCAGAGC 
CAGGCCCAGG TCCTCAGCCA GGAGGGGAAG GACACAGATG AGGTGGACGG CCAGCGCCGC 
ATCGTGGAGA AGAAGTTCCA GCAGCTGCTA GACCCACTGA AGAAGAGGAA GGGAAACCTC 
ATGGCCTCCC GCGAGATCCA CCAGTTCAAC AGAGACGTTG AGGACGAGAT CCTCTGGGTT 
GAAGAGAGAA TGCCACTGGC AACATCTACA GACCACGGAA ACAACCTGCA AACAGTCCAG 
TTACTCATCA AGAAAAACCA GACCCTGCAA AAGGAGATCC AGGGCCACCA GCCGCGCTGT 
GACGACATCT TTGAGCGGAG TCAGCAGGTG CTGAAGGATG ACGACAACAG CCCCAGCGGC 
GAGGGGATCC GCCAGCGGCT GGCCGACCTG CGGCGCCTTT GGGCCCAGAT CCTGGAGGAG 
ACAGCCAAAC GCCATGGACG CCTGGAGGAG GCCCACAAGG CCCAGCAGTA CTACTTTGAT 
GCTGCCGAGG CTGAGGCCTG GATGAGCGAG CAGGAGCTCT ATATGATGTC AGAGGAGAAA 
GCCAAGGATG AGCAGAGCTC CGTGGCCATG CTGAAGAAGC ACCAGATCCT GGAGCAGGCT 
GTGGAGGACT ATGCTGAGAC TGTCCACCAG CTCTCCAAGA CCAGCAGGGG CCTGACGGCC 
GCCGGACACC CAGAAAGTGA ACGGATCGGC ATGCGTCAGT CCCAGGTGGA TAAGCTGTAC 
GCGGGGCTCA AGGACCTGTC GGAGGAGAGG AGGGGGAAGT TGGACGAGAG GTTCCGTCTT 
TTTCAGCTCA ACAGGGAGGT GGATGATCTG GAGCAGTGGA TCGCTGAAAG GGAGGTGGTG 
GCTGGGTCAC ATGAGCTGGG ACAGGACTAT GAACATGTCA CGATGCTTCA GGAGCGTTTC 
CGTGAGTTTG CTCGCGACAC AGGCAACATT GGTCAAGAGC GTGTGGACAC TGTCAACCAG 
CTGGCGGACG AACTCATCAA CTCGGGCCAC GCCGACGCGG CTACCATCGC CGAGTGGAAG 
GACGGGCTGA ATGAGGCGTG GGCCGACCTG CTGGAGCTGA TTGACACACG CACGCAGATC 
CTGGCGGCGT CCTTCGAGCT GCACAAGTTC TACCACGACG CCAAGGAGAT CCTCAACCGC 
ATCCTGGACA AACACAAGAA GCTGCCTGAG GAGCTGGGCC GGGACCAGAA CACAGTGGAG 
ACCCTGCAGA GGATGCACAC CACCTTCGAA CACGACATCC AAGCCCTGGG GACACAGGTG 
AGGATGTTGC AGGAGGATGC TGTGCGCCTG CAGTCTGCCT ATGCCGGAGA CAAGGCTGAT 
GACATCCAAA AGAGAGAGGG TGAGGTTTTG GAGGCCTGGA GAACACTGCT GGAGGCCTGT 
GACGGCCGCA GGGTCAGACT GCTCGACACC GGCGACAAGT TCCGTTTCTT CAGCATGGTC 
CGAGACCTCA TGCTCTGGAT GGAGGACGTC ATCCGCCTCA TCGAGGCTCA GGAGAAGCCC 
AGGGACGTGT CGTCTGTGGA GCTCCTGATG AACAACCACC AGGGCATCAA GGCTGAGATC 
GACGCCCGTA ACGACAGCTT TACCAACTGC ATCGAGCTGG GCAAGTCCCT GTTGGCACGA 
AAACACTACG CATTAGAGGA GATCAAAGAG AAGTTACTAC AGTTGACGGA CAAGAGGAAA 
GACATGATTG ACAAGTGGGA GGACAGATGG GAGTGGCTTA GATTGGTTCT GGAGGTGCAC 
CAGTTTGGAA GGGACGCGGG TGTGGCCGAG TCGTGGCTGC TGGGACAGGA GCCGTACCTG 
TCCAGCAGGG AGATGGGTCA GAACGTGGAT GAAGTGGAGA AACTCATCAA GAGACACGAG 
GCCTTTGAGA AGTCAGCTGC CACCTGGGAA GAGCGGTTCT CTGCGCTGGA ACGGCTGACT 
ACGATGGAAT TACTGGAAGT GAGAAGACAG CAAGAGGAGG AGGAGAGGAG GAGAAATCCC 
CCACCTGCAG AGACCCAGAC GGACACTGCA TCGCCACAGC AGAAGGAGGG TGAGCAAGTG 
TCTCTGAATG GACTGCCATC GGACCAGGAC TCCCCTCGGG TTAGTTACCG CTCCCCAGCC 
TACCAAAAGT ACTCCAGCTT TCAAACCCGC AGGACTGGGA CTGGGCGAGA AAGGCGGGCG 
GGCTGGACCG TGACCCCCCA ATGCCCCCCT TGCCCTTTGA ACCCCCTGCC CCCACCACAG 
GGAGAGGACT GTACACCCCT CTACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110502571 ( XM_021580713.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021436388.1 Oncorhynchus mykiss spectrin beta chain, non-erythrocytic 1-like (LOC110502571), transcript variant X2, mRNA.

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CloneID	OOf29776
Clone ID Related Accession (Same CDS sequence)	XM_021580713.1
Accession Version	XM_021580713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	spectrin beta chain, non-erythrocytic 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035099.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCTG TAGCCGCCGA TTACGACCAC ATGGAGATCC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGCAGCAGTA CAGCAGCGAC ATTGTCAACA ACCGCTGGGA TGCTGACGAG 
TGGGACAACG AGAACAGCTC GGCCAGGCTG TTTGAACGCT CCCGCATCAA GGCCTTGGCA 
GATGAGCGTG AAGCGGTGCA GAAGAAGACC TTCACCAAGT GGGTCAACTC CCACCTAGCC 
AGAGTGTCCT GCAGGATCAC TGACCTCTAT ATGGACCTGA GAGATGGACG CATGCTCATC 
AAACTACTGG AGGTTCTCTC TGGGGAGAAA CTGCCCAAGC CCACTAAGGG GCGCATGCGT 
ATCCACTGCC TGGAGAACGT GGACAAGGCC CTGCAGTTCC TCAAGGAGCA GAGGGTCCAC 
CTAGAAAACA TGGGCTCTCA TGACATCGTT GATGGCAACC ACCGCCTCAC CCTTGGCCTC 
ATCTGGACCA TCATCCTCCG ATTCCAGATC CAGGACATCA GAGTGGAGAC GGAGGATAAC 
AAGGAGAAGA AGTCGGCTAA AGATGCTCTG CTGCTCTGGT GCCAGATGAA GACCGCAGGA 
TATCCCAACG TAAACATTCA CAACTTCAGC ACCAGCTGGA GGGATGGCAT GGCTTTCAAC 
GCCATCCTCC ACAAACACAG ACCTGACCTG ATAGACTTTG ACAAGTTGAA AAAGTCAAAT 
GCCCACCACA ACCTGCAGAA TGCCTTCAAC CTGGCAGAGC AACACCTGGG CCTCCACAAG 
CTACTGGACG TAGAGGATAT CAGCGTGGAC CACCCTGATG AGAAGTCCAT CATCACCTAT 
GTTGTGACCT ACTACCACTA CTTCTCCAAG ATGAAGGCCC TGAAGGTGGA GGGCAAACGG 
ATTGGGAAGG TGCTGGACAA TGCCATTGAG ACCGATAACA TGATTCAGAA ATATGAGAGT 
CTGGCCTCTG ACCTCCTGGA ATGGATTGAA CAGACCATCA TCATCCTCAA CAACCGCAGG 
TTTGCCAACT CCCTAGTGGG GGTGCAGCAA CAGCTCCAGG CCTTCAACAC CTACCGTACC 
GTCGAAAAGC CACCCAAGTT CACTGAGAAA GGCAACCTGG AGGTTCTCCT CTTCACCATC 
CAGAGCAAGA TGAGGGCCAA CAATCAGAAG GTCTACATCC CGCGGGACGG AAAACTCATC 
TCAGACATCA ACAAGGCCTG GGAGAGGCTG GAGAAGTCAG AGCATGAGAG GGAGCTGGCT 
CTAAGGACTG AGCTGATTCG TCAGGAGAAA CTGGAGCAGC TGGCCCGCCG CTTTGACCGC 
AAAGCCGCCA TGAGAGAGAC CTGGCTGAGT GAGAACCAAC GGCTCGTCTC ACAGGACAAC 
TTTGGCTTCG ACCTGCAGGC GGTGGAGGCC GCCACCAAGA AGCACGAGGC CATCGAGACG 
GACATCGGCG CCTACGAGGA GCGTGTGCAA GCAGTGCTCT CCGTGGCCAA GGAGCTGGAG 
GCAGAGAACT ACCATGACAT CAAGCGCGTG ACAGCGAGGA AGGACAACGT GCTGCGTCTG 
TGGGAGTACC TGTTGGAGCT GCTGAAGGCC CGGCGCATCC GGCTGGAGAT GAACCTGGGC 
CTGCAGAGGG TCTTCCAGGA GATGCTTTAC ATCATGGACT GGATGGATGA GATGAAGATG 
CTGCTGCTGT CTCAGGACTA TGGGAAACAC CTGCTGGGAG TGGAGGACCT GCTGCAGAAG 
CACACTCTGG TGGAGGCTGA TATTGCCATC CAGGCCGACC GCGTCAAAGC CGTCAACAAC 
AACGCTCAGA AGTTCGCCAT TGATGCAGAG GGCTACAAGC CCTGTGACCC CCAGGTGATC 
CGGGACCGAG TGGCCCACAT GGAGTTCTGT TACGCAGAGC TGAGCCAGCT GGCCGCCGAT 
CGCTGCGCCC GCCTGGAGGA GTCCCGCCGC CTTTGGAAGT TCTTCTGGGA GATGGCCGAG 
GAGGAGGGCT GGATCCGTGA GAAAGAGCAG ATCCTGTCCT CGGAGGACTG TGGTAAGGAT 
CTGACGGGCA CCGTGCGTCT GCTGAGCCAG CACCGCGCCT TCGAGGATGA GATGATCGGC 
CGCGCCGGCC ACCTGCAACA GACCATCAAG CAGGGAGAGG CGCTGGTGGC CGCCGACCAC 
TTCGGAGCCG ACAAGATCCG TGAGCGCATC CAAGACATCC AGGAGCAGTG GGCGGCCTTG 
GAGAGGCTAT CTGCCGTTAG GAAGGCCCGG CTGGAGGAGG CCTGCAACCT GCACCAGTTC 
CAGGCTGATG CCGACGACAT CGACGCCTGG ATGCTGGATG TTCTCCGCAT TGTGTCCAGT 
GCCGACGTGG GCCACGACGA GTTCTCCACC CAGGCCCTGG TCAAGAAGCA CAAGGATGTG 
GCCGAGGAGA TTGCCAGCTA CCGACCTGTC ATCGACGCCC TGCACGAGCA GTCCAAAACC 
CTGCCCGGGG AACAGACTGG CTCAGAGGAG GTGCAGGGGC GCCTGTCGGG CATCGAGGAG 
CGCTATAAGG AGGTGGCTGA GCTGACCCGG CTGAGGAAGC AGGCCCTACA GGATGCCCTG 
GCCCTCTACA AGATGTTCAG CGAGGCGGAT GCCTGTGAGG TGTGGATCGA TGAGAAGGAG 
CAGTGGCTCA ACAGTATGGA GATACCAGAG AAACTGGAGG ATCTGGAAGT CGTTCAGCAC 
AGGTTTGAAA GTCTGGAGCC AGAGATGAAC AACCAAGCCT CACGCGTTGC CGTGGTGAAC 
CAGATTGCTA GGCAACTCAT CCACAGTGGT CACCCCAGTG AAAAGGAGAT CAAAGCCCAA 
CAGGACAAAC TCAACACCAG ATGGAGTCAG TTCCGTGACC TAGTTGACCT GAAGAAAGAC 
TCCCTCAACT CAGCTCTGGG TGTACAGAAC TACCACCTGG AGTGTAACGA GACCAAGTCG 
TGGATCCGCG AGAAGACCAA GGTGATCGAG TCCACCCAGG AGCTGGGTAA TGACCTGGCC 
GGGGTCATGG CCCTGCAGCG CAAGCTCACC GGCATGGAGC GTGACCTGAC CGCCATCGAG 
GACAAGCTAG GAGAACTGGG GAAGGAGGGG GAGCACCTTG CAGCCGAGCA CCCTGACCAG 
GCCCAGGGCA TCATGGGTCG CCTGGGGGAG ATCACAGGAG TGTGGGACGA GATGAAGGGC 
ACCCTGAAGA ACCGCGAGGA GTCCCTGGGC GAGGCCAGCA AGCTGCAGCA GTTCCTCCGG 
GAGCTGGATG ACTTCCAGTC ATGGCTTTCC AAGACCCAGA CGGCCATCGC TTCAGAAGAC 
ATGCCCAACA CACTGACGGA GGCCGAAAAG CTGCTGGCGC AGCATGAGGG CATCAAGAAC 
GAGATCCGCA ACTATGAAGT GGACTACCAG AAGATGAGGG ACATGGGGGA AATGGTGACC 
CAGGGCCAGA CGGACGCCCA GTACATGTTC CTGAGGCAGA GGCTGCAGGC CCTGGACACA 
GGCTGGAACG AGCTGCACAA GATGTGGGAG AACCGTCAGA ACCTGTTGTC CCAGTCCCAT 
GCCCACCAGC TGTTCCTCAG GGATACCAAG CAGGCTGAAG CCTTCCTCAA CAACCAGGAG 
TACGTGCTGG CCCATACTGA GATGCCCACA ACTCTGGAGG GGGCAGAGGG AGCCATTAAG 
AAGCAGGAGG ACTTCATGAC CACCATGGAC GCCAACGAGG AGAAGATCAA TGCCGTGGTG 
GACACCGGAC GCAGACTGGT CAGTGACAAA AACATCAATG CTGACCGCAT CCAGGAGAAA 
GTGGACTCTA TTGATGAGAG ACATCAGAAG AACCGCTTGG CGGCCAGTGA GCTTCTCTGT 
AAGCTGAAGG ATAACAGAGA CCTGCAGAAG TTCCTTCAGA ACTGCCAAGA GCTGTCCCTG 
TGGATCAATG AGAAGATGCT CACGGCTCAG GACATGTCGT ACGACGAAGC CAGGAACCTC 
CACAGCAAAT GGCTCAAACA CCAGGCCTTC ATGGCCGAGC TTCAGTCCAA CAAGGAGTGG 
CTGGACGAGA TCGAGAAGGA CGGGATGCAG CTGATAGCTG AGAAGCCGGA GACCGAGGCC 
ATGGTGAAGG AAAAGCTGGC AGCGCTCCAT AAGATGTGGG ATGACCTTGA GTCCACCACC 
CAGACTAAGG CCAAGTGTCT GTTTGACGCC AACAAGGCTG AGCTGTTCAC CCAGAGTTGT 
GCCGACCTGG ACAAGTGGCT GGTGGGAGTG GATGGACAAA TCCAATCAGA TGACTACGGC 
AAAGATCTGA CCAGCGTCAA CATCCTGCTC AAGAAACAAC AGATGCTGGA GAGCCAGGTG 
GAGGTGCGTC AGAAGGAGGT GGAGGAGCTG CAGAGCCAGG CCCAGGTCCT CAGCCAGGAG 
GGGAAGGACA CAGATGAGGT GGACGGCCAG CGCCGCATCG TGGAGAAGAA GTTCCAGCAG 
CTGCTAGACC CACTGAAGAA GAGGAAGGGA AACCTCATGG CCTCCCGCGA GATCCACCAG 
TTCAACAGAG ACGTTGAGGA CGAGATCCTC TGGGTTGAAG AGAGAATGCC ACTGGCAACA 
TCTACAGACC ACGGAAACAA CCTGCAAACA GTCCAGTTAC TCATCAAGAA AAACCAGACC 
CTGCAAAAGG AGATCCAGGG CCACCAGCCG CGCTGTGACG ACATCTTTGA GCGGAGTCAG 
CAGGTGCTGA AGGATGACGA CAACAGCCCC AGCGGCGAGG GGATCCGCCA GCGGCTGGCC 
GACCTGCGGC GCCTTTGGGC CCAGATCCTG GAGGAGACAG CCAAACGCCA TGGACGCCTG 
GAGGAGGCCC ACAAGGCCCA GCAGTACTAC TTTGATGCTG CCGAGGCTGA GGCCTGGATG 
AGCGAGCAGG AGCTCTATAT GATGTCAGAG GAGAAAGCCA AGGATGAGCA GAGCTCCGTG 
GCCATGCTGA AGAAGCACCA GATCCTGGAG CAGGCTGTGG AGGACTATGC TGAGACTGTC 
CACCAGCTCT CCAAGACCAG CAGGGGCCTG ACGGCCGCCG GACACCCAGA AAGTGAACGG 
ATCGGCATGC GTCAGTCCCA GGTGGATAAG CTGTACGCGG GGCTCAAGGA CCTGTCGGAG 
GAGAGGAGGG GGAAGTTGGA CGAGAGGTTC CGTCTTTTTC AGCTCAACAG GGAGGTGGAT 
GATCTGGAGC AGTGGATCGC TGAAAGGGAG GTGGTGGCTG GGTCACATGA GCTGGGACAG 
GACTATGAAC ATGTCACGAT GCTTCAGGAG CGTTTCCGTG AGTTTGCTCG CGACACAGGC 
AACATTGGTC AAGAGCGTGT GGACACTGTC AACCAGCTGG CGGACGAACT CATCAACTCG 
GGCCACGCCG ACGCGGCTAC CATCGCCGAG TGGAAGGACG GGCTGAATGA GGCGTGGGCC 
GACCTGCTGG AGCTGATTGA CACACGCACG CAGATCCTGG CGGCGTCCTT CGAGCTGCAC 
AAGTTCTACC ACGACGCCAA GGAGATCCTC AACCGCATCC TGGACAAACA CAAGAAGCTG 
CCTGAGGAGC TGGGCCGGGA CCAGAACACA GTGGAGACCC TGCAGAGGAT GCACACCACC 
TTCGAACACG ACATCCAAGC CCTGGGGACA CAGGTGAGGA TGTTGCAGGA GGATGCTGTG 
CGCCTGCAGT CTGCCTATGC CGGAGACAAG GCTGATGACA TCCAAAAGAG AGAGGGTGAG 
GTTTTGGAGG CCTGGAGAAC ACTGCTGGAG GCCTGTGACG GCCGCAGGGT CAGACTGCTC 
GACACCGGCG ACAAGTTCCG TTTCTTCAGC ATGGTCCGAG ACCTCATGCT CTGGATGGAG 
GACGTCATCC GCCTCATCGA GGCTCAGGAG AAGCCCAGGG ACGTGTCGTC TGTGGAGCTC 
CTGATGAACA ACCACCAGGG CATCAAGGCT GAGATCGACG CCCGTAACGA CAGCTTTACC 
AACTGCATCG AGCTGGGCAA GTCCCTGTTG GCACGAAAAC ACTACGCATT AGAGGAGATC 
AAAGAGAAGT TACTACAGTT GACGGACAAG AGGAAAGACA TGATTGACAA GTGGGAGGAC 
AGATGGGAGT GGCTTAGATT GGTTCTGGAG GTGCACCAGT TTGGAAGGGA CGCGGGTGTG 
GCCGAGTCGT GGCTGCTGGG ACAGGAGCCG TACCTGTCCA GCAGGGAGAT GGGTCAGAAC 
GTGGATGAAG TGGAGAAACT CATCAAGAGA CACGAGGCCT TTGAGAAGTC AGCTGCCACC 
TGGGAAGAGC GGTTCTCTGC GCTGGAACGG CTGACTACGA TGGAATTACT GGAAGTGAGA 
AGACAGCAAG AGGAGGAGGA GAGGAGGAGA AATCCCCCAC CTGCAGAGAC CCAGACGGAC 
ACTGCATCGC CACAGCAGAA GGAGGGTGAG CAAGTGTCTC TGAATGGACT GCCATCGGAC 
CAGGACTCCC CTCGGGACAA TGGGGAGGGA AGTGACATTG TAAATGGGGT GGCGGAGCCC 
AGCCCAGGCG GGTCGCCCAG TGCAGCACAC AAGGGCAAGC CCAGCCAGGC TGCCACACTC 
CCAGCCAAGT CCCTGGACTC AACCCCAGCC AACCAGATGG AAGGCCTCCT GCACCGCAAA 
CATGAGTGGG CACGACATAA TCGTAAACAC AGTTTCAGAT CCTGGCACAA TGTGTATTGT 
GTGATCAACA ACTGGGAAAT GGGCTTCTAC AAAGACATTA AGAGTGCCAG CCAGGGCATC 
CCCTATCACA GCGAGATCCC CATCAGCCTG AAGGACGCTG CGTGTGAGAT CGCCCTCGAT 
TACAAGAAGA AGAAGCATGT GTTCAAACTG AAAGCTACAG ATGGGAATGA GTATCTCTTC 
CAAGCCAAAG ATGACGAGGA GATGAACTCG TGGATGGAGG CCATCTCCAG TGCTGCGTCC 
TCTGATAAGA CCGAGATGAC CCCCAGCAGC CATAGCACCC CCGCCTCCAG CCGAGCCCAG 
ACCTTGCCCG CCTCTGTTGC CCTGGCAACC GAATCCAGCC CAGGCAAGCG CGAGAAAGAC 
AAAGAAAAAC GCTTCAGCCT TTTCAGCAAA AAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021436388.1
CDS	199..7257
Translation	MTSVAADYDHMEIQQQQQQQQQQQQQYSSDIVNNRWDADEWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYMDLRDGRMLIKLLEVLSGEKLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDIRVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFSTSWRDGMAFNAILHKHRPDLIDFDKLKKSNAHHNLQNAFNLAEQHLGLHKLLDVEDISVDHPDEKSIITYVVTYYHYFSKMKALKVEGKRIGKVLDNAIETDNMIQKYESLASDLLEWIEQTIIILNNRRFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYIPRDGKLISDINKAWERLEKSEHERELALRTELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLQAVEAATKKHEAIETDIGAYEERVQAVLSVAKELEAENYHDIKRVTARKDNVLRLWEYLLELLKARRIRLEMNLGLQRVFQEMLYIMDWMDEMKMLLLSQDYGKHLLGVEDLLQKHTLVEADIAIQADRVKAVNNNAQKFAIDAEGYKPCDPQVIRDRVAHMEFCYAELSQLAADRCARLEESRRLWKFFWEMAEEEGWIREKEQILSSEDCGKDLTGTVRLLSQHRAFEDEMIGRAGHLQQTIKQGEALVAADHFGADKIRERIQDIQEQWAALERLSAVRKARLEEACNLHQFQADADDIDAWMLDVLRIVSSADVGHDEFSTQALVKKHKDVAEEIASYRPVIDALHEQSKTLPGEQTGSEEVQGRLSGIEERYKEVAELTRLRKQALQDALALYKMFSEADACEVWIDEKEQWLNSMEIPEKLEDLEVVQHRFESLEPEMNNQASRVAVVNQIARQLIHSGHPSEKEIKAQQDKLNTRWSQFRDLVDLKKDSLNSALGVQNYHLECNETKSWIREKTKVIESTQELGNDLAGVMALQRKLTGMERDLTAIEDKLGELGKEGEHLAAEHPDQAQGIMGRLGEITGVWDEMKGTLKNREESLGEASKLQQFLRELDDFQSWLSKTQTAIASEDMPNTLTEAEKLLAQHEGIKNEIRNYEVDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAHQLFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEGAIKKQEDFMTTMDANEEKINAVVDTGRRLVSDKNINADRIQEKVDSIDERHQKNRLAASELLCKLKDNRDLQKFLQNCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELQSNKEWLDEIEKDGMQLIAEKPETEAMVKEKLAALHKMWDDLESTTQTKAKCLFDANKAELFTQSCADLDKWLVGVDGQIQSDDYGKDLTSVNILLKKQQMLESQVEVRQKEVEELQSQAQVLSQEGKDTDEVDGQRRIVEKKFQQLLDPLKKRKGNLMASREIHQFNRDVEDEILWVEERMPLATSTDHGNNLQTVQLLIKKNQTLQKEIQGHQPRCDDIFERSQQVLKDDDNSPSGEGIRQRLADLRRLWAQILEETAKRHGRLEEAHKAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSSVAMLKKHQILEQAVEDYAETVHQLSKTSRGLTAAGHPESERIGMRQSQVDKLYAGLKDLSEERRGKLDERFRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNQLADELINSGHADAATIAEWKDGLNEAWADLLELIDTRTQILAASFELHKFYHDAKEILNRILDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRMLQEDAVRLQSAYAGDKADDIQKREGEVLEAWRTLLEACDGRRVRLLDTGDKFRFFSMVRDLMLWMEDVIRLIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTNCIELGKSLLARKHYALEEIKEKLLQLTDKRKDMIDKWEDRWEWLRLVLEVHQFGRDAGVAESWLLGQEPYLSSREMGQNVDEVEKLIKRHEAFEKSAATWEERFSALERLTTMELLEVRRQQEEEERRRNPPPAETQTDTASPQQKEGEQVSLNGLPSDQDSPRDNGEGSDIVNGVAEPSPGGSPSAAHKGKPSQAATLPAKSLDSTPANQMEGLLHRKHEWARHNRKHSFRSWHNVYCVINNWEMGFYKDIKSASQGIPYHSEIPISLKDAACEIALDYKKKKHVFKLKATDGNEYLFQAKDDEEMNSWMEAISSAASSDKTEMTPSSHSTPASSRAQTLPASVALATESSPGKREKDKEKRFSLFSKKK

Target ORF information:

RefSeq Version	XM_021580713.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss spectrin beta chain, non-erythrocytic 1-like (LOC110502571), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021580713.1

ORF Insert Sequence:

ATGACATCTG TAGCCGCCGA TTACGACCAC ATGGAGATCC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGCAGCAGTA CAGCAGCGAC ATTGTCAACA ACCGCTGGGA TGCTGACGAG 
TGGGACAACG AGAACAGCTC GGCCAGGCTG TTTGAACGCT CCCGCATCAA GGCCTTGGCA 
GATGAGCGTG AAGCGGTGCA GAAGAAGACC TTCACCAAGT GGGTCAACTC CCACCTAGCC 
AGAGTGTCCT GCAGGATCAC TGACCTCTAT ATGGACCTGA GAGATGGACG CATGCTCATC 
AAACTACTGG AGGTTCTCTC TGGGGAGAAA CTGCCCAAGC CCACTAAGGG GCGCATGCGT 
ATCCACTGCC TGGAGAACGT GGACAAGGCC CTGCAGTTCC TCAAGGAGCA GAGGGTCCAC 
CTAGAAAACA TGGGCTCTCA TGACATCGTT GATGGCAACC ACCGCCTCAC CCTTGGCCTC 
ATCTGGACCA TCATCCTCCG ATTCCAGATC CAGGACATCA GAGTGGAGAC GGAGGATAAC 
AAGGAGAAGA AGTCGGCTAA AGATGCTCTG CTGCTCTGGT GCCAGATGAA GACCGCAGGA 
TATCCCAACG TAAACATTCA CAACTTCAGC ACCAGCTGGA GGGATGGCAT GGCTTTCAAC 
GCCATCCTCC ACAAACACAG ACCTGACCTG ATAGACTTTG ACAAGTTGAA AAAGTCAAAT 
GCCCACCACA ACCTGCAGAA TGCCTTCAAC CTGGCAGAGC AACACCTGGG CCTCCACAAG 
CTACTGGACG TAGAGGATAT CAGCGTGGAC CACCCTGATG AGAAGTCCAT CATCACCTAT 
GTTGTGACCT ACTACCACTA CTTCTCCAAG ATGAAGGCCC TGAAGGTGGA GGGCAAACGG 
ATTGGGAAGG TGCTGGACAA TGCCATTGAG ACCGATAACA TGATTCAGAA ATATGAGAGT 
CTGGCCTCTG ACCTCCTGGA ATGGATTGAA CAGACCATCA TCATCCTCAA CAACCGCAGG 
TTTGCCAACT CCCTAGTGGG GGTGCAGCAA CAGCTCCAGG CCTTCAACAC CTACCGTACC 
GTCGAAAAGC CACCCAAGTT CACTGAGAAA GGCAACCTGG AGGTTCTCCT CTTCACCATC 
CAGAGCAAGA TGAGGGCCAA CAATCAGAAG GTCTACATCC CGCGGGACGG AAAACTCATC 
TCAGACATCA ACAAGGCCTG GGAGAGGCTG GAGAAGTCAG AGCATGAGAG GGAGCTGGCT 
CTAAGGACTG AGCTGATTCG TCAGGAGAAA CTGGAGCAGC TGGCCCGCCG CTTTGACCGC 
AAAGCCGCCA TGAGAGAGAC CTGGCTGAGT GAGAACCAAC GGCTCGTCTC ACAGGACAAC 
TTTGGCTTCG ACCTGCAGGC GGTGGAGGCC GCCACCAAGA AGCACGAGGC CATCGAGACG 
GACATCGGCG CCTACGAGGA GCGTGTGCAA GCAGTGCTCT CCGTGGCCAA GGAGCTGGAG 
GCAGAGAACT ACCATGACAT CAAGCGCGTG ACAGCGAGGA AGGACAACGT GCTGCGTCTG 
TGGGAGTACC TGTTGGAGCT GCTGAAGGCC CGGCGCATCC GGCTGGAGAT GAACCTGGGC 
CTGCAGAGGG TCTTCCAGGA GATGCTTTAC ATCATGGACT GGATGGATGA GATGAAGATG 
CTGCTGCTGT CTCAGGACTA TGGGAAACAC CTGCTGGGAG TGGAGGACCT GCTGCAGAAG 
CACACTCTGG TGGAGGCTGA TATTGCCATC CAGGCCGACC GCGTCAAAGC CGTCAACAAC 
AACGCTCAGA AGTTCGCCAT TGATGCAGAG GGCTACAAGC CCTGTGACCC CCAGGTGATC 
CGGGACCGAG TGGCCCACAT GGAGTTCTGT TACGCAGAGC TGAGCCAGCT GGCCGCCGAT 
CGCTGCGCCC GCCTGGAGGA GTCCCGCCGC CTTTGGAAGT TCTTCTGGGA GATGGCCGAG 
GAGGAGGGCT GGATCCGTGA GAAAGAGCAG ATCCTGTCCT CGGAGGACTG TGGTAAGGAT 
CTGACGGGCA CCGTGCGTCT GCTGAGCCAG CACCGCGCCT TCGAGGATGA GATGATCGGC 
CGCGCCGGCC ACCTGCAACA GACCATCAAG CAGGGAGAGG CGCTGGTGGC CGCCGACCAC 
TTCGGAGCCG ACAAGATCCG TGAGCGCATC CAAGACATCC AGGAGCAGTG GGCGGCCTTG 
GAGAGGCTAT CTGCCGTTAG GAAGGCCCGG CTGGAGGAGG CCTGCAACCT GCACCAGTTC 
CAGGCTGATG CCGACGACAT CGACGCCTGG ATGCTGGATG TTCTCCGCAT TGTGTCCAGT 
GCCGACGTGG GCCACGACGA GTTCTCCACC CAGGCCCTGG TCAAGAAGCA CAAGGATGTG 
GCCGAGGAGA TTGCCAGCTA CCGACCTGTC ATCGACGCCC TGCACGAGCA GTCCAAAACC 
CTGCCCGGGG AACAGACTGG CTCAGAGGAG GTGCAGGGGC GCCTGTCGGG CATCGAGGAG 
CGCTATAAGG AGGTGGCTGA GCTGACCCGG CTGAGGAAGC AGGCCCTACA GGATGCCCTG 
GCCCTCTACA AGATGTTCAG CGAGGCGGAT GCCTGTGAGG TGTGGATCGA TGAGAAGGAG 
CAGTGGCTCA ACAGTATGGA GATACCAGAG AAACTGGAGG ATCTGGAAGT CGTTCAGCAC 
AGGTTTGAAA GTCTGGAGCC AGAGATGAAC AACCAAGCCT CACGCGTTGC CGTGGTGAAC 
CAGATTGCTA GGCAACTCAT CCACAGTGGT CACCCCAGTG AAAAGGAGAT CAAAGCCCAA 
CAGGACAAAC TCAACACCAG ATGGAGTCAG TTCCGTGACC TAGTTGACCT GAAGAAAGAC 
TCCCTCAACT CAGCTCTGGG TGTACAGAAC TACCACCTGG AGTGTAACGA GACCAAGTCG 
TGGATCCGCG AGAAGACCAA GGTGATCGAG TCCACCCAGG AGCTGGGTAA TGACCTGGCC 
GGGGTCATGG CCCTGCAGCG CAAGCTCACC GGCATGGAGC GTGACCTGAC CGCCATCGAG 
GACAAGCTAG GAGAACTGGG GAAGGAGGGG GAGCACCTTG CAGCCGAGCA CCCTGACCAG 
GCCCAGGGCA TCATGGGTCG CCTGGGGGAG ATCACAGGAG TGTGGGACGA GATGAAGGGC 
ACCCTGAAGA ACCGCGAGGA GTCCCTGGGC GAGGCCAGCA AGCTGCAGCA GTTCCTCCGG 
GAGCTGGATG ACTTCCAGTC ATGGCTTTCC AAGACCCAGA CGGCCATCGC TTCAGAAGAC 
ATGCCCAACA CACTGACGGA GGCCGAAAAG CTGCTGGCGC AGCATGAGGG CATCAAGAAC 
GAGATCCGCA ACTATGAAGT GGACTACCAG AAGATGAGGG ACATGGGGGA AATGGTGACC 
CAGGGCCAGA CGGACGCCCA GTACATGTTC CTGAGGCAGA GGCTGCAGGC CCTGGACACA 
GGCTGGAACG AGCTGCACAA GATGTGGGAG AACCGTCAGA ACCTGTTGTC CCAGTCCCAT 
GCCCACCAGC TGTTCCTCAG GGATACCAAG CAGGCTGAAG CCTTCCTCAA CAACCAGGAG 
TACGTGCTGG CCCATACTGA GATGCCCACA ACTCTGGAGG GGGCAGAGGG AGCCATTAAG 
AAGCAGGAGG ACTTCATGAC CACCATGGAC GCCAACGAGG AGAAGATCAA TGCCGTGGTG 
GACACCGGAC GCAGACTGGT CAGTGACAAA AACATCAATG CTGACCGCAT CCAGGAGAAA 
GTGGACTCTA TTGATGAGAG ACATCAGAAG AACCGCTTGG CGGCCAGTGA GCTTCTCTGT 
AAGCTGAAGG ATAACAGAGA CCTGCAGAAG TTCCTTCAGA ACTGCCAAGA GCTGTCCCTG 
TGGATCAATG AGAAGATGCT CACGGCTCAG GACATGTCGT ACGACGAAGC CAGGAACCTC 
CACAGCAAAT GGCTCAAACA CCAGGCCTTC ATGGCCGAGC TTCAGTCCAA CAAGGAGTGG 
CTGGACGAGA TCGAGAAGGA CGGGATGCAG CTGATAGCTG AGAAGCCGGA GACCGAGGCC 
ATGGTGAAGG AAAAGCTGGC AGCGCTCCAT AAGATGTGGG ATGACCTTGA GTCCACCACC 
CAGACTAAGG CCAAGTGTCT GTTTGACGCC AACAAGGCTG AGCTGTTCAC CCAGAGTTGT 
GCCGACCTGG ACAAGTGGCT GGTGGGAGTG GATGGACAAA TCCAATCAGA TGACTACGGC 
AAAGATCTGA CCAGCGTCAA CATCCTGCTC AAGAAACAAC AGATGCTGGA GAGCCAGGTG 
GAGGTGCGTC AGAAGGAGGT GGAGGAGCTG CAGAGCCAGG CCCAGGTCCT CAGCCAGGAG 
GGGAAGGACA CAGATGAGGT GGACGGCCAG CGCCGCATCG TGGAGAAGAA GTTCCAGCAG 
CTGCTAGACC CACTGAAGAA GAGGAAGGGA AACCTCATGG CCTCCCGCGA GATCCACCAG 
TTCAACAGAG ACGTTGAGGA CGAGATCCTC TGGGTTGAAG AGAGAATGCC ACTGGCAACA 
TCTACAGACC ACGGAAACAA CCTGCAAACA GTCCAGTTAC TCATCAAGAA AAACCAGACC 
CTGCAAAAGG AGATCCAGGG CCACCAGCCG CGCTGTGACG ACATCTTTGA GCGGAGTCAG 
CAGGTGCTGA AGGATGACGA CAACAGCCCC AGCGGCGAGG GGATCCGCCA GCGGCTGGCC 
GACCTGCGGC GCCTTTGGGC CCAGATCCTG GAGGAGACAG CCAAACGCCA TGGACGCCTG 
GAGGAGGCCC ACAAGGCCCA GCAGTACTAC TTTGATGCTG CCGAGGCTGA GGCCTGGATG 
AGCGAGCAGG AGCTCTATAT GATGTCAGAG GAGAAAGCCA AGGATGAGCA GAGCTCCGTG 
GCCATGCTGA AGAAGCACCA GATCCTGGAG CAGGCTGTGG AGGACTATGC TGAGACTGTC 
CACCAGCTCT CCAAGACCAG CAGGGGCCTG ACGGCCGCCG GACACCCAGA AAGTGAACGG 
ATCGGCATGC GTCAGTCCCA GGTGGATAAG CTGTACGCGG GGCTCAAGGA CCTGTCGGAG 
GAGAGGAGGG GGAAGTTGGA CGAGAGGTTC CGTCTTTTTC AGCTCAACAG GGAGGTGGAT 
GATCTGGAGC AGTGGATCGC TGAAAGGGAG GTGGTGGCTG GGTCACATGA GCTGGGACAG 
GACTATGAAC ATGTCACGAT GCTTCAGGAG CGTTTCCGTG AGTTTGCTCG CGACACAGGC 
AACATTGGTC AAGAGCGTGT GGACACTGTC AACCAGCTGG CGGACGAACT CATCAACTCG 
GGCCACGCCG ACGCGGCTAC CATCGCCGAG TGGAAGGACG GGCTGAATGA GGCGTGGGCC 
GACCTGCTGG AGCTGATTGA CACACGCACG CAGATCCTGG CGGCGTCCTT CGAGCTGCAC 
AAGTTCTACC ACGACGCCAA GGAGATCCTC AACCGCATCC TGGACAAACA CAAGAAGCTG 
CCTGAGGAGC TGGGCCGGGA CCAGAACACA GTGGAGACCC TGCAGAGGAT GCACACCACC 
TTCGAACACG ACATCCAAGC CCTGGGGACA CAGGTGAGGA TGTTGCAGGA GGATGCTGTG 
CGCCTGCAGT CTGCCTATGC CGGAGACAAG GCTGATGACA TCCAAAAGAG AGAGGGTGAG 
GTTTTGGAGG CCTGGAGAAC ACTGCTGGAG GCCTGTGACG GCCGCAGGGT CAGACTGCTC 
GACACCGGCG ACAAGTTCCG TTTCTTCAGC ATGGTCCGAG ACCTCATGCT CTGGATGGAG 
GACGTCATCC GCCTCATCGA GGCTCAGGAG AAGCCCAGGG ACGTGTCGTC TGTGGAGCTC 
CTGATGAACA ACCACCAGGG CATCAAGGCT GAGATCGACG CCCGTAACGA CAGCTTTACC 
AACTGCATCG AGCTGGGCAA GTCCCTGTTG GCACGAAAAC ACTACGCATT AGAGGAGATC 
AAAGAGAAGT TACTACAGTT GACGGACAAG AGGAAAGACA TGATTGACAA GTGGGAGGAC 
AGATGGGAGT GGCTTAGATT GGTTCTGGAG GTGCACCAGT TTGGAAGGGA CGCGGGTGTG 
GCCGAGTCGT GGCTGCTGGG ACAGGAGCCG TACCTGTCCA GCAGGGAGAT GGGTCAGAAC 
GTGGATGAAG TGGAGAAACT CATCAAGAGA CACGAGGCCT TTGAGAAGTC AGCTGCCACC 
TGGGAAGAGC GGTTCTCTGC GCTGGAACGG CTGACTACGA TGGAATTACT GGAAGTGAGA 
AGACAGCAAG AGGAGGAGGA GAGGAGGAGA AATCCCCCAC CTGCAGAGAC CCAGACGGAC 
ACTGCATCGC CACAGCAGAA GGAGGGTGAG CAAGTGTCTC TGAATGGACT GCCATCGGAC 
CAGGACTCCC CTCGGGACAA TGGGGAGGGA AGTGACATTG TAAATGGGGT GGCGGAGCCC 
AGCCCAGGCG GGTCGCCCAG TGCAGCACAC AAGGGCAAGC CCAGCCAGGC TGCCACACTC 
CCAGCCAAGT CCCTGGACTC AACCCCAGCC AACCAGATGG AAGGCCTCCT GCACCGCAAA 
CATGAGTGGG CACGACATAA TCGTAAACAC AGTTTCAGAT CCTGGCACAA TGTGTATTGT 
GTGATCAACA ACTGGGAAAT GGGCTTCTAC AAAGACATTA AGAGTGCCAG CCAGGGCATC 
CCCTATCACA GCGAGATCCC CATCAGCCTG AAGGACGCTG CGTGTGAGAT CGCCCTCGAT 
TACAAGAAGA AGAAGCATGT GTTCAAACTG AAAGCTACAG ATGGGAATGA GTATCTCTTC 
CAAGCCAAAG ATGACGAGGA GATGAACTCG TGGATGGAGG CCATCTCCAG TGCTGCGTCC 
TCTGATAAGA CCGAGATGAC CCCCAGCAGC CATAGCACCC CCGCCTCCAG CCGAGCCCAG 
ACCTTGCCCG CCTCTGTTGC CCTGGCAACC GAATCCAGCC CAGGCAAGCG CGAGAAAGAC 
AAAGAAAAAC GCTTCAGCCT TTTCAGCAAA AAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC110502571 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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