LOC110500643 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110500643
Entrez Gene ID	110500643
Full Name	DENN domain-containing protein 4C-like
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021578125.1	XP_021433800.1	DENN domain-containing protein 4C-like isoform X1
XM_021578126.1	XP_021433801.1	DENN domain-containing protein 4C-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC110500643 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110500643 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf27701	XM_021578125.1 Latest version!	Oncorhynchus mykiss DENN domain-containing protein 4C-like (LOC110500643), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOf27702	XM_021578126.1 Latest version!	Oncorhynchus mykiss DENN domain-containing protein 4C-like (LOC110500643), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110500643 ( XM_021578125.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021433800.1 Oncorhynchus mykiss DENN domain-containing protein 4C-like (LOC110500643), transcript variant X1, mRNA.

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CloneID	OOf27701
Clone ID Related Accession (Same CDS sequence)	XM_021578125.1
Accession Version	XM_021578125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	DENN domain-containing protein 4C-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGAGG ACAAGGGTCA CCGTGTGACC GACTACTTTG TGGTGGCCGG GCTGACCGAC 
AAGTCCACGC CACTGGAGCA GGACCTGTCG GAGACCAAGT CCAGCGGGCC CAAGGCGCCC 
ATCACTGACC TGGCTGTCAT CAACAAGTCA GCGGGCGAGA CGGTGCCGGA GGGCTTCACC 
TGCATCGAGA GCACCTACAG CGCGCAGCAG GCCAACCTCA ACCATGGCAG CCTTAAGAGC 
CCCGAGATAT TTCTCTGCTA CAAGAGGGGC CACGACAAGC CCCCGCTCAT TGACATCGGG 
GTGTTATATG AAGGTAAGGA GCGTCTCATC CAGGGCTGTG AGGTAATCCA GGCCACTCCG 
TATGGCCGCT GTGCCAACGT CAACAACAGT TCTGCCACCT CGCAGCGCAT CTTCATCACC 
TTACGCCGTG CGCCACCCGT CCAGCCCCAG AACTCCCTGT CCGTCACCGA CATCTGCGTC 
ATCGTCACCA GCAAGGGCGA GACGCCGCCA CACACCTTCT GCAAGGTGGA CAAGAACCTC 
AACTGTGGCA TGTGGGGCTC TAGTGTTTTC CTGTGCTACA AGAAATCCGT GTCGGTGTCC 
AACTCCATTA ATTACAAAGC CGGCCTCATC TTCCGCTACC CAGAGGATGA CTATGAGTCC 
TTCCCCCTGT CGGAGTCTGT GCCCCTGTTC TGCCTCCCCA TGGGGGCTAA GATCGAATGC 
TGGACCCCCA ACACACGCTA CCCTCTCCCC GTCTTCTCCA CCTTTGTGTT AACCAACTCC 
TCTGGGGAAA AGGTGTACGG TGCTGCCATC CAGTTCTATG AGGCCTACCC CATAGATCTC 
CTCAGTGAGA AGCAGAAGGT GCAGTTGGGC CTGCTCACCA CCGTGGAGAA GAAAATGATC 
CCCAACCGGA CAGTCAACAC CAACAAGTGC ATCTGCCTCC TTTCCCGCTG GCCCTTCTTC 
GAGTCGTTCC GCAAATTCCT CATGTTCCTC TACAAGCTGT CCGTCTCGGG CCCCCACCCG 
CTGCCCATCG AAAAGCACAT CTCCCATTTC ATGCACAACG TGTCCTTCCC CTCACCTCAG 
AGGCCCAGAA TCTTGGTCCA GCTCTCTGCA CACGACACCT TGATGCTCGC ACAGCCTGTG 
TGTACTCCTA TGCCTCTCAG TGGTGCAGAC TACAGTACAC TGCTGATTAA CCTGGGCTCA 
GAGAACTGTG CCACGCTGTT GCACTTTGTC CTGCTGGAGA GTAAGATCCT GCTGCACTCC 
TTGAGACCCG CTGTACTCAC CGGGGTGGCA GAGGCTGTGG TGGCGATGAT TTTTCCATTC 
CAGTGGCAGT GCCCGTACAT CCCCCTCTGC CCGCTCTCTC TGGCGGGGGT CCTCAACGCA 
CCCTGTCCCT TCATTGTGGG CGTGGACTCC CGCTACTTTG ACCTCTATGA CCCTCCTCCG 
GACGTGGTCT GTGTGGACCT GGACACCAAC ACGATCTACC TGTCTGATGA GAAGAAACAC 
AGCAACTGGA AGAACCTCCC AAAGAAGCCC TGCAAGAGTC TGTTTAACTC CCTGACCAAC 
CTGCAACACC AGCTGGGCAT CGTGCGTCGG ACGGCCCAGG AGGGCTTGGC GGTGGAGATG 
ACCCCCATCG AGGCGGACTT CACCTGGCAC AAGAAGATGA CGTCCCTGGA GATGGAGATC 
CAAGAGGCCC TGCTGCGCTT CATGGCCCAC ATCCTGAAGG GCTACCGTTC CTATCTCAAA 
CCCATCACCC AGGCCCCCTC CGAGAAGGCC ACCTCTGCAG ACTCACTATA TGACCTACAG 
GGTTTCCTAA AGAGCAGGGA GCGAGCCCAC CAGAAGTTCT ATTCGCAGCT GACCAAGACT 
CAGATCTTTA TACGCTTCAT CGAGGAGTGC ACATTCGTCA GCGACAAGGA TACAGGCCTT 
GCCTTCTTCG ACGACTGCAT TGAGAAGCTA TTTCCCTCTG ATAAAGGCAA CGACAAAGGC 
ACTAAGGTGG AGGGTGAGTC CTCGGAGGAC ACTAAGCTCA TGGAACTAGA CGAATCACAG 
AGGAGTGAGC ATACAGTCTT CGTCATGCCC CCAGAACCTC CCACCACCGA GGACGAATCT 
GATCCCCCTA CCCGCTACAG TTATAAGAGT TTCCCTCGGT TGAGTATGGA CCTGTTTGAC 
TGTCCCAAAG AGGTGATACC AGCGCTGAGG AGCAGTACAC CAGGGGCCAG CCTGTCCAGC 
AGCCCTGCAC TGCTGGCCAA GAGGACCAAG CAGGAGATCA AGCTGGCCTA CAAGATGGCC 
AAGCGCTTCT ACTCCGACCC TCCGCTGTGG GCCAAGTGTC TGTTCAGCCA CTGCTACAGT 
CTGTGGTTCA TCTGTCTGCC GGTGGGGGTG AGGCTGGTCC AGTCAAAGAG CTGGGCCATG 
CAGCAGGCCT ACAACGTCCT GCTCAAGATG AGGACGTCTG AGGTGGAGGT CCTGGATGAG 
GTGTGTTACC GTGTAGTGAT GCAGCTGTGT GGTTTGTACG GTCTGCCGGT CATGGCCGTC 
CGTGTTCTGG TGGAGATGAA GAAGGCAGGG GTGCACCCTA ACGCGATCAC CTATGGCTAC 
TACAACAAGG CTGTTCTGGA AGGCCCCTGG CCAAGTCGGA ACCGCAGCGG TCTCTTCATG 
TGGGCCAAGG TTCGCAACGT GGTGCGTACT GTGGCCCAAT TTAAACAGGC ACTCCACCGG 
ATGCCCGCCA CGAAAGGACC CACCATCATC ACAACAGTGG TGTCGGTGAG CGGCGACCTG 
CTGAGCCACG GGAGTGCAGA CCACTCCAGT GAGGTGAACG GAGAGGATCA CACGCTGTTC 
GCACGCAGCC TCATCATAGG AGACGCCACG GACAACCACT GCAGCACAGG AGGTCAGTCT 
GACCAGGGCT ACGGCTCTAA AGATGAGCTG CATCAGGTGC AAGTGGATTT GGCTCCATCA 
GCAGCCCCTC TTGTCAATGT GGATCACCGC AGCAAGCCCA AAGCACAGCA CAATGATGGG 
GACATAGCTG GCTCGGTGGA CAGTTCTGTG GTCGTAGCGG AGCCCACCAG CCCTGTTGAA 
GCCATCCCAC ATGTCCCCAG CATCGTCAAG CTGTCCACAG GAGGGAGCTT CGATACTGCC 
ATGAAGAGAG GAGAAACTGC CCCAGGGAAG CTGTTCTCTC TGCGCAGCCA GAGCGAGAAT 
GTCTCTCTGC CGGAGGAGGG TGAGTCTACA GCCCCGGGTG GCTCTGGGGA GGCGGGGCCT 
GCAGTTCAGG GGCAGGGCCA GCGCCAGAAG GCCTTCTCGG AACGCAGCTG CAGCTTCAGC 
GTCGAGAGTC GTGCCGGCAT GCTGATGGAG AAAAATGGTG TGGACCACAT CGCGAGACGC 
ATGGGTGCCG ATGCCCGAAT CCTCGCCGAT GCCCTTTCTG GGTGTGGCAC CACCCAACCA 
CCTACAAATC CACCATCCAA GGCCCTTTTT AAGGAGTTGG AGTCAGAGGA GGGGCGTTGT 
CCGATGCTGG GGAAACTGTC AGAGGAGGAG GGTCCTGTAA TGGACCCAAC CCCATTGGAG 
GAGGGGCTAG GGAAAGAGGA GGAGAGAATG GACGGGGAGG ATGAGATGGG ACTGAAGTTG 
GAAGAGGAAG GAGATTCAGG GGGGAGGGGT GATAGCTCTC TCCCAACCCT GGAGATGAAG 
GAGGTGGATG TGGGGGCTGA TCCCCTCTCC CTGCTGATCT CAGAGCACGA AGAGTTGGCC 
TCCGTAACCA GCCAGGAGCT GCCGCACTCC CTGCCCGCCG TGGTGTCCCG AAACCTGGCA 
GACGAGATTG AGATGTACAT GAGCCTGCGA AGCCCACTGG GCCCCAAGTC CTCCAGTATG 
GAGTTCCACC AGGGGGGCCA GGGAGGGGAC TCTTCCCCGG ACACTGCCCC AGTTAAGCAA 
GCTCTAGAAC GGAGGTCCAG CCTCCCTGTG CCCCCTGTCA AACACCCAGG GGGTTCAAGC 
GGGGACTCAA CGCCCAAACG CAGCCCTGCT GCTGTCACGC GCTCCAAGAC ATTCGCCGTA 
ATAGCCACCA GGAGCCGCGG TGTTGTGGCC AGCCCGAGAT CGTCACTGAC CGCGCTGGTG 
AGGTCATCGC AAAATGGCTC GCTGGGCTCG GTCATCAACT CCATCTCAGG CATGAAGATG 
GACACCCTGC TCTCTGGTCC CAAGATGGAC GTGTTGAAGT CAGGCATGAA GCAGGCAGGG 
AACCTGGCCA GCAAAGTGTG GGGGGCTGTG GCCTCAGCCT ATTCCTACTC CGATGACGAG 
GATGAGCTGG CCCCGGGCGA TAGGGATAGT TTCCCTGCCG GGCTGGATGA GTCCATGCTG 
TCAGGGGGTG GAGGAGGGGA CAGTCCCACC CACAGAGGGC CAACCCAAGG CCTGGTGTCC 
AACGGACTGA CACAGAGCAG CACCAGCCTG GCCAGCAGCA GTAGCAGCAG TGACACAGGG 
AGAGGACCTC ACTCCACACT GACTCCAGGA CGGCCAGGAA GAGGTCCAGA CTCTGATCAA 
GGCTCCCACC ACACCAGCTC CTCAAGTATC TACCAGAACT GTGCCCTAGA GGTGCTGATG 
TCCAGCTGCT CCCTGTGTCG GTCCTGTGAG TGTCTGGTGT ACGACGAGGA GATCATGGCG 
GGCTGGACGG CAGACGACTC CAACCTCAAC ACCACCTGTC CTTTCTGCCG CGCCGCCTTT 
CTGCCCCTGC TCCACGTCGA GTTCCACGAC CTGCGCACCA ACACAGCGTT CTACATGAAG 
CCCAGTGCAT CAGGAGACAG TATCCACAGC GCCAGCCACC AGCCCACAAC TACAGACTCC 
ACCGTGCTCG TGGTGGGGGG TGCAGCAGAC ACCCCGCGCC CGGACCTCAT CAGCTTCCCA 
GAATCCTCCT CTGAGGACCC AGGGGAGGCC CCGGCCAGCC AAACAGCCAC TCACAAGAGT 
TTGGTACCGG TACCGGAGCC GGCTCAGTCG GACCCTCTGG GCTTGCTGGA ACACCAGGCA 
GGGAAGCGTG GGACTTCTCT GACGCGCAGT AACAGCGTGG GCGGGCCACT ACAGAGCCTG 
GTCTCTTACC AGAGACAACC CGGACACGGC GTGTCCACCA CCAGCCTGCC CTGCAGCCTG 
ACAGAGGTGG ATGGCCTGGG ACAAAAGCGG CCCAACCCCA TGCCAGTGTC TGTGCCCTAC 
CTCAGCCCCC TGGTCCTCCG CAAAGAACTG GAGACCCTAC TAGAGAACGA GGGGGACCAG 
GTGATCTACA CCCATAAATT CCTGAGTCAA CACCCCATCA TCTTCTGGAA CCTGGTGTTT 
TATTTCTGTC GCCTGGACCT GCCCAGCTAC CTGCCTGGCC TCATCCTCAC CTCAGAACAC 
TGCAACAATG GAGTACAGCT CCCCCAGACC TCCCTGTCCC AGGACAGTAA ACAGGTGTAT 
GTGCAGCTGC TGTGGGACAA CATCAACCTG CACCAGGAGC CTGGCGATCC CCTCTACCAG 
AGCTGGAGGA CCCTGCTGGA GCAGAAGGCC ACCCTGGCCC CCACGGACCA TCAGGAGACA 
CGGACCTTCC TCAACAGCAT CGTCCGCAGC ATCCAGACGA ACGACGTATA CGGCCCCATC 
AATCTGCTCC TCCGAGAGGT CAAGCGGCAC CCGGAGGTCA AACGCCAAAG GGGTATCTAT 
AGGGAGATCC TCTTCCTGGC GCTTGTGGCA TTGGGACGGG AGAACATTGA CGTCGAGGCC 
TTTGACCGTG AATACCGCCA GGCCTTTGAT GATCTGAGCA CAGAGCAGCT CAAGGCTCTG 
CACAAGATTG ACCGGCCACC CAACAGCAAT GTCCAGTGGT GCCTCAAGTG CTTTGGAGCT 
CCAGTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021433800.1
CDS	438..6269
Translation	MIEDKGHRVTDYFVVAGLTDKSTPLEQDLSETKSSGPKAPITDLAVINKSAGETVPEGFTCIESTYSAQQANLNHGSLKSPEIFLCYKRGHDKPPLIDIGVLYEGKERLIQGCEVIQATPYGRCANVNNSSATSQRIFITLRRAPPVQPQNSLSVTDICVIVTSKGETPPHTFCKVDKNLNCGMWGSSVFLCYKKSVSVSNSINYKAGLIFRYPEDDYESFPLSESVPLFCLPMGAKIECWTPNTRYPLPVFSTFVLTNSSGEKVYGAAIQFYEAYPIDLLSEKQKVQLGLLTTVEKKMIPNRTVNTNKCICLLSRWPFFESFRKFLMFLYKLSVSGPHPLPIEKHISHFMHNVSFPSPQRPRILVQLSAHDTLMLAQPVCTPMPLSGADYSTLLINLGSENCATLLHFVLLESKILLHSLRPAVLTGVAEAVVAMIFPFQWQCPYIPLCPLSLAGVLNAPCPFIVGVDSRYFDLYDPPPDVVCVDLDTNTIYLSDEKKHSNWKNLPKKPCKSLFNSLTNLQHQLGIVRRTAQEGLAVEMTPIEADFTWHKKMTSLEMEIQEALLRFMAHILKGYRSYLKPITQAPSEKATSADSLYDLQGFLKSRERAHQKFYSQLTKTQIFIRFIEECTFVSDKDTGLAFFDDCIEKLFPSDKGNDKGTKVEGESSEDTKLMELDESQRSEHTVFVMPPEPPTTEDESDPPTRYSYKSFPRLSMDLFDCPKEVIPALRSSTPGASLSSSPALLAKRTKQEIKLAYKMAKRFYSDPPLWAKCLFSHCYSLWFICLPVGVRLVQSKSWAMQQAYNVLLKMRTSEVEVLDEVCYRVVMQLCGLYGLPVMAVRVLVEMKKAGVHPNAITYGYYNKAVLEGPWPSRNRSGLFMWAKVRNVVRTVAQFKQALHRMPATKGPTIITTVVSVSGDLLSHGSADHSSEVNGEDHTLFARSLIIGDATDNHCSTGGQSDQGYGSKDELHQVQVDLAPSAAPLVNVDHRSKPKAQHNDGDIAGSVDSSVVVAEPTSPVEAIPHVPSIVKLSTGGSFDTAMKRGETAPGKLFSLRSQSENVSLPEEGESTAPGGSGEAGPAVQGQGQRQKAFSERSCSFSVESRAGMLMEKNGVDHIARRMGADARILADALSGCGTTQPPTNPPSKALFKELESEEGRCPMLGKLSEEEGPVMDPTPLEEGLGKEEERMDGEDEMGLKLEEEGDSGGRGDSSLPTLEMKEVDVGADPLSLLISEHEELASVTSQELPHSLPAVVSRNLADEIEMYMSLRSPLGPKSSSMEFHQGGQGGDSSPDTAPVKQALERRSSLPVPPVKHPGGSSGDSTPKRSPAAVTRSKTFAVIATRSRGVVASPRSSLTALVRSSQNGSLGSVINSISGMKMDTLLSGPKMDVLKSGMKQAGNLASKVWGAVASAYSYSDDEDELAPGDRDSFPAGLDESMLSGGGGGDSPTHRGPTQGLVSNGLTQSSTSLASSSSSSDTGRGPHSTLTPGRPGRGPDSDQGSHHTSSSSIYQNCALEVLMSSCSLCRSCECLVYDEEIMAGWTADDSNLNTTCPFCRAAFLPLLHVEFHDLRTNTAFYMKPSASGDSIHSASHQPTTTDSTVLVVGGAADTPRPDLISFPESSSEDPGEAPASQTATHKSLVPVPEPAQSDPLGLLEHQAGKRGTSLTRSNSVGGPLQSLVSYQRQPGHGVSTTSLPCSLTEVDGLGQKRPNPMPVSVPYLSPLVLRKELETLLENEGDQVIYTHKFLSQHPIIFWNLVFYFCRLDLPSYLPGLILTSEHCNNGVQLPQTSLSQDSKQVYVQLLWDNINLHQEPGDPLYQSWRTLLEQKATLAPTDHQETRTFLNSIVRSIQTNDVYGPINLLLREVKRHPEVKRQRGIYREILFLALVALGRENIDVEAFDREYRQAFDDLSTEQLKALHKIDRPPNSNVQWCLKCFGAPVI

Target ORF information:

RefSeq Version	XM_021578125.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss DENN domain-containing protein 4C-like (LOC110500643), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021578125.1

ORF Insert Sequence:

ATGATCGAGG ACAAGGGTCA CCGTGTGACC GACTACTTTG TGGTGGCCGG GCTGACCGAC 
AAGTCCACGC CACTGGAGCA GGACCTGTCG GAGACCAAGT CCAGCGGGCC CAAGGCGCCC 
ATCACTGACC TGGCTGTCAT CAACAAGTCA GCGGGCGAGA CGGTGCCGGA GGGCTTCACC 
TGCATCGAGA GCACCTACAG CGCGCAGCAG GCCAACCTCA ACCATGGCAG CCTTAAGAGC 
CCCGAGATAT TTCTCTGCTA CAAGAGGGGC CACGACAAGC CCCCGCTCAT TGACATCGGG 
GTGTTATATG AAGGTAAGGA GCGTCTCATC CAGGGCTGTG AGGTAATCCA GGCCACTCCG 
TATGGCCGCT GTGCCAACGT CAACAACAGT TCTGCCACCT CGCAGCGCAT CTTCATCACC 
TTACGCCGTG CGCCACCCGT CCAGCCCCAG AACTCCCTGT CCGTCACCGA CATCTGCGTC 
ATCGTCACCA GCAAGGGCGA GACGCCGCCA CACACCTTCT GCAAGGTGGA CAAGAACCTC 
AACTGTGGCA TGTGGGGCTC TAGTGTTTTC CTGTGCTACA AGAAATCCGT GTCGGTGTCC 
AACTCCATTA ATTACAAAGC CGGCCTCATC TTCCGCTACC CAGAGGATGA CTATGAGTCC 
TTCCCCCTGT CGGAGTCTGT GCCCCTGTTC TGCCTCCCCA TGGGGGCTAA GATCGAATGC 
TGGACCCCCA ACACACGCTA CCCTCTCCCC GTCTTCTCCA CCTTTGTGTT AACCAACTCC 
TCTGGGGAAA AGGTGTACGG TGCTGCCATC CAGTTCTATG AGGCCTACCC CATAGATCTC 
CTCAGTGAGA AGCAGAAGGT GCAGTTGGGC CTGCTCACCA CCGTGGAGAA GAAAATGATC 
CCCAACCGGA CAGTCAACAC CAACAAGTGC ATCTGCCTCC TTTCCCGCTG GCCCTTCTTC 
GAGTCGTTCC GCAAATTCCT CATGTTCCTC TACAAGCTGT CCGTCTCGGG CCCCCACCCG 
CTGCCCATCG AAAAGCACAT CTCCCATTTC ATGCACAACG TGTCCTTCCC CTCACCTCAG 
AGGCCCAGAA TCTTGGTCCA GCTCTCTGCA CACGACACCT TGATGCTCGC ACAGCCTGTG 
TGTACTCCTA TGCCTCTCAG TGGTGCAGAC TACAGTACAC TGCTGATTAA CCTGGGCTCA 
GAGAACTGTG CCACGCTGTT GCACTTTGTC CTGCTGGAGA GTAAGATCCT GCTGCACTCC 
TTGAGACCCG CTGTACTCAC CGGGGTGGCA GAGGCTGTGG TGGCGATGAT TTTTCCATTC 
CAGTGGCAGT GCCCGTACAT CCCCCTCTGC CCGCTCTCTC TGGCGGGGGT CCTCAACGCA 
CCCTGTCCCT TCATTGTGGG CGTGGACTCC CGCTACTTTG ACCTCTATGA CCCTCCTCCG 
GACGTGGTCT GTGTGGACCT GGACACCAAC ACGATCTACC TGTCTGATGA GAAGAAACAC 
AGCAACTGGA AGAACCTCCC AAAGAAGCCC TGCAAGAGTC TGTTTAACTC CCTGACCAAC 
CTGCAACACC AGCTGGGCAT CGTGCGTCGG ACGGCCCAGG AGGGCTTGGC GGTGGAGATG 
ACCCCCATCG AGGCGGACTT CACCTGGCAC AAGAAGATGA CGTCCCTGGA GATGGAGATC 
CAAGAGGCCC TGCTGCGCTT CATGGCCCAC ATCCTGAAGG GCTACCGTTC CTATCTCAAA 
CCCATCACCC AGGCCCCCTC CGAGAAGGCC ACCTCTGCAG ACTCACTATA TGACCTACAG 
GGTTTCCTAA AGAGCAGGGA GCGAGCCCAC CAGAAGTTCT ATTCGCAGCT GACCAAGACT 
CAGATCTTTA TACGCTTCAT CGAGGAGTGC ACATTCGTCA GCGACAAGGA TACAGGCCTT 
GCCTTCTTCG ACGACTGCAT TGAGAAGCTA TTTCCCTCTG ATAAAGGCAA CGACAAAGGC 
ACTAAGGTGG AGGGTGAGTC CTCGGAGGAC ACTAAGCTCA TGGAACTAGA CGAATCACAG 
AGGAGTGAGC ATACAGTCTT CGTCATGCCC CCAGAACCTC CCACCACCGA GGACGAATCT 
GATCCCCCTA CCCGCTACAG TTATAAGAGT TTCCCTCGGT TGAGTATGGA CCTGTTTGAC 
TGTCCCAAAG AGGTGATACC AGCGCTGAGG AGCAGTACAC CAGGGGCCAG CCTGTCCAGC 
AGCCCTGCAC TGCTGGCCAA GAGGACCAAG CAGGAGATCA AGCTGGCCTA CAAGATGGCC 
AAGCGCTTCT ACTCCGACCC TCCGCTGTGG GCCAAGTGTC TGTTCAGCCA CTGCTACAGT 
CTGTGGTTCA TCTGTCTGCC GGTGGGGGTG AGGCTGGTCC AGTCAAAGAG CTGGGCCATG 
CAGCAGGCCT ACAACGTCCT GCTCAAGATG AGGACGTCTG AGGTGGAGGT CCTGGATGAG 
GTGTGTTACC GTGTAGTGAT GCAGCTGTGT GGTTTGTACG GTCTGCCGGT CATGGCCGTC 
CGTGTTCTGG TGGAGATGAA GAAGGCAGGG GTGCACCCTA ACGCGATCAC CTATGGCTAC 
TACAACAAGG CTGTTCTGGA AGGCCCCTGG CCAAGTCGGA ACCGCAGCGG TCTCTTCATG 
TGGGCCAAGG TTCGCAACGT GGTGCGTACT GTGGCCCAAT TTAAACAGGC ACTCCACCGG 
ATGCCCGCCA CGAAAGGACC CACCATCATC ACAACAGTGG TGTCGGTGAG CGGCGACCTG 
CTGAGCCACG GGAGTGCAGA CCACTCCAGT GAGGTGAACG GAGAGGATCA CACGCTGTTC 
GCACGCAGCC TCATCATAGG AGACGCCACG GACAACCACT GCAGCACAGG AGGTCAGTCT 
GACCAGGGCT ACGGCTCTAA AGATGAGCTG CATCAGGTGC AAGTGGATTT GGCTCCATCA 
GCAGCCCCTC TTGTCAATGT GGATCACCGC AGCAAGCCCA AAGCACAGCA CAATGATGGG 
GACATAGCTG GCTCGGTGGA CAGTTCTGTG GTCGTAGCGG AGCCCACCAG CCCTGTTGAA 
GCCATCCCAC ATGTCCCCAG CATCGTCAAG CTGTCCACAG GAGGGAGCTT CGATACTGCC 
ATGAAGAGAG GAGAAACTGC CCCAGGGAAG CTGTTCTCTC TGCGCAGCCA GAGCGAGAAT 
GTCTCTCTGC CGGAGGAGGG TGAGTCTACA GCCCCGGGTG GCTCTGGGGA GGCGGGGCCT 
GCAGTTCAGG GGCAGGGCCA GCGCCAGAAG GCCTTCTCGG AACGCAGCTG CAGCTTCAGC 
GTCGAGAGTC GTGCCGGCAT GCTGATGGAG AAAAATGGTG TGGACCACAT CGCGAGACGC 
ATGGGTGCCG ATGCCCGAAT CCTCGCCGAT GCCCTTTCTG GGTGTGGCAC CACCCAACCA 
CCTACAAATC CACCATCCAA GGCCCTTTTT AAGGAGTTGG AGTCAGAGGA GGGGCGTTGT 
CCGATGCTGG GGAAACTGTC AGAGGAGGAG GGTCCTGTAA TGGACCCAAC CCCATTGGAG 
GAGGGGCTAG GGAAAGAGGA GGAGAGAATG GACGGGGAGG ATGAGATGGG ACTGAAGTTG 
GAAGAGGAAG GAGATTCAGG GGGGAGGGGT GATAGCTCTC TCCCAACCCT GGAGATGAAG 
GAGGTGGATG TGGGGGCTGA TCCCCTCTCC CTGCTGATCT CAGAGCACGA AGAGTTGGCC 
TCCGTAACCA GCCAGGAGCT GCCGCACTCC CTGCCCGCCG TGGTGTCCCG AAACCTGGCA 
GACGAGATTG AGATGTACAT GAGCCTGCGA AGCCCACTGG GCCCCAAGTC CTCCAGTATG 
GAGTTCCACC AGGGGGGCCA GGGAGGGGAC TCTTCCCCGG ACACTGCCCC AGTTAAGCAA 
GCTCTAGAAC GGAGGTCCAG CCTCCCTGTG CCCCCTGTCA AACACCCAGG GGGTTCAAGC 
GGGGACTCAA CGCCCAAACG CAGCCCTGCT GCTGTCACGC GCTCCAAGAC ATTCGCCGTA 
ATAGCCACCA GGAGCCGCGG TGTTGTGGCC AGCCCGAGAT CGTCACTGAC CGCGCTGGTG 
AGGTCATCGC AAAATGGCTC GCTGGGCTCG GTCATCAACT CCATCTCAGG CATGAAGATG 
GACACCCTGC TCTCTGGTCC CAAGATGGAC GTGTTGAAGT CAGGCATGAA GCAGGCAGGG 
AACCTGGCCA GCAAAGTGTG GGGGGCTGTG GCCTCAGCCT ATTCCTACTC CGATGACGAG 
GATGAGCTGG CCCCGGGCGA TAGGGATAGT TTCCCTGCCG GGCTGGATGA GTCCATGCTG 
TCAGGGGGTG GAGGAGGGGA CAGTCCCACC CACAGAGGGC CAACCCAAGG CCTGGTGTCC 
AACGGACTGA CACAGAGCAG CACCAGCCTG GCCAGCAGCA GTAGCAGCAG TGACACAGGG 
AGAGGACCTC ACTCCACACT GACTCCAGGA CGGCCAGGAA GAGGTCCAGA CTCTGATCAA 
GGCTCCCACC ACACCAGCTC CTCAAGTATC TACCAGAACT GTGCCCTAGA GGTGCTGATG 
TCCAGCTGCT CCCTGTGTCG GTCCTGTGAG TGTCTGGTGT ACGACGAGGA GATCATGGCG 
GGCTGGACGG CAGACGACTC CAACCTCAAC ACCACCTGTC CTTTCTGCCG CGCCGCCTTT 
CTGCCCCTGC TCCACGTCGA GTTCCACGAC CTGCGCACCA ACACAGCGTT CTACATGAAG 
CCCAGTGCAT CAGGAGACAG TATCCACAGC GCCAGCCACC AGCCCACAAC TACAGACTCC 
ACCGTGCTCG TGGTGGGGGG TGCAGCAGAC ACCCCGCGCC CGGACCTCAT CAGCTTCCCA 
GAATCCTCCT CTGAGGACCC AGGGGAGGCC CCGGCCAGCC AAACAGCCAC TCACAAGAGT 
TTGGTACCGG TACCGGAGCC GGCTCAGTCG GACCCTCTGG GCTTGCTGGA ACACCAGGCA 
GGGAAGCGTG GGACTTCTCT GACGCGCAGT AACAGCGTGG GCGGGCCACT ACAGAGCCTG 
GTCTCTTACC AGAGACAACC CGGACACGGC GTGTCCACCA CCAGCCTGCC CTGCAGCCTG 
ACAGAGGTGG ATGGCCTGGG ACAAAAGCGG CCCAACCCCA TGCCAGTGTC TGTGCCCTAC 
CTCAGCCCCC TGGTCCTCCG CAAAGAACTG GAGACCCTAC TAGAGAACGA GGGGGACCAG 
GTGATCTACA CCCATAAATT CCTGAGTCAA CACCCCATCA TCTTCTGGAA CCTGGTGTTT 
TATTTCTGTC GCCTGGACCT GCCCAGCTAC CTGCCTGGCC TCATCCTCAC CTCAGAACAC 
TGCAACAATG GAGTACAGCT CCCCCAGACC TCCCTGTCCC AGGACAGTAA ACAGGTGTAT 
GTGCAGCTGC TGTGGGACAA CATCAACCTG CACCAGGAGC CTGGCGATCC CCTCTACCAG 
AGCTGGAGGA CCCTGCTGGA GCAGAAGGCC ACCCTGGCCC CCACGGACCA TCAGGAGACA 
CGGACCTTCC TCAACAGCAT CGTCCGCAGC ATCCAGACGA ACGACGTATA CGGCCCCATC 
AATCTGCTCC TCCGAGAGGT CAAGCGGCAC CCGGAGGTCA AACGCCAAAG GGGTATCTAT 
AGGGAGATCC TCTTCCTGGC GCTTGTGGCA TTGGGACGGG AGAACATTGA CGTCGAGGCC 
TTTGACCGTG AATACCGCCA GGCCTTTGAT GATCTGAGCA CAGAGCAGCT CAAGGCTCTG 
CACAAGATTG ACCGGCCACC CAACAGCAAT GTCCAGTGGT GCCTCAAGTG CTTTGGAGCT 
CCAGTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110500643 ( XM_021578126.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021433801.1 Oncorhynchus mykiss DENN domain-containing protein 4C-like (LOC110500643), transcript variant X2, mRNA.

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CloneID	OOf27702
Clone ID Related Accession (Same CDS sequence)	XM_021578126.1
Accession Version	XM_021578126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	DENN domain-containing protein 4C-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCT CTAGTGTTTT CCTGTGCTAC AAGAAATCCG TGTCGGTGTC CAACTCCATT 
AATTACAAAG CCGGCCTCAT CTTCCGCTAC CCAGAGGATG ACTATGAGTC CTTCCCCCTG 
TCGGAGTCTG TGCCCCTGTT CTGCCTCCCC ATGGGGGCTA AGATCGAATG CTGGACCCCC 
AACACACGCT ACCCTCTCCC CGTCTTCTCC ACCTTTGTGT TAACCAACTC CTCTGGGGAA 
AAGGTGTACG GTGCTGCCAT CCAGTTCTAT GAGGCCTACC CCATAGATCT CCTCAGTGAG 
AAGCAGAAGG TGCAGTTGGG CCTGCTCACC ACCGTGGAGA AGAAAATGAT CCCCAACCGG 
ACAGTCAACA CCAACAAGTG CATCTGCCTC CTTTCCCGCT GGCCCTTCTT CGAGTCGTTC 
CGCAAATTCC TCATGTTCCT CTACAAGCTG TCCGTCTCGG GCCCCCACCC GCTGCCCATC 
GAAAAGCACA TCTCCCATTT CATGCACAAC GTGTCCTTCC CCTCACCTCA GAGGCCCAGA 
ATCTTGGTCC AGCTCTCTGC ACACGACACC TTGATGCTCG CACAGCCTGT GTGTACTCCT 
ATGCCTCTCA GTGGTGCAGA CTACAGTACA CTGCTGATTA ACCTGGGCTC AGAGAACTGT 
GCCACGCTGT TGCACTTTGT CCTGCTGGAG AGTAAGATCC TGCTGCACTC CTTGAGACCC 
GCTGTACTCA CCGGGGTGGC AGAGGCTGTG GTGGCGATGA TTTTTCCATT CCAGTGGCAG 
TGCCCGTACA TCCCCCTCTG CCCGCTCTCT CTGGCGGGGG TCCTCAACGC ACCCTGTCCC 
TTCATTGTGG GCGTGGACTC CCGCTACTTT GACCTCTATG ACCCTCCTCC GGACGTGGTC 
TGTGTGGACC TGGACACCAA CACGATCTAC CTGTCTGATG AGAAGAAACA CAGCAACTGG 
AAGAACCTCC CAAAGAAGCC CTGCAAGAGT CTGTTTAACT CCCTGACCAA CCTGCAACAC 
CAGCTGGGCA TCGTGCGTCG GACGGCCCAG GAGGGCTTGG CGGTGGAGAT GACCCCCATC 
GAGGCGGACT TCACCTGGCA CAAGAAGATG ACGTCCCTGG AGATGGAGAT CCAAGAGGCC 
CTGCTGCGCT TCATGGCCCA CATCCTGAAG GGCTACCGTT CCTATCTCAA ACCCATCACC 
CAGGCCCCCT CCGAGAAGGC CACCTCTGCA GACTCACTAT ATGACCTACA GGGTTTCCTA 
AAGAGCAGGG AGCGAGCCCA CCAGAAGTTC TATTCGCAGC TGACCAAGAC TCAGATCTTT 
ATACGCTTCA TCGAGGAGTG CACATTCGTC AGCGACAAGG ATACAGGCCT TGCCTTCTTC 
GACGACTGCA TTGAGAAGCT ATTTCCCTCT GATAAAGGCA ACGACAAAGG CACTAAGGTG 
GAGGGTGAGT CCTCGGAGGA CACTAAGCTC ATGGAACTAG ACGAATCACA GAGGAGTGAG 
CATACAGTCT TCGTCATGCC CCCAGAACCT CCCACCACCG AGGACGAATC TGATCCCCCT 
ACCCGCTACA GTTATAAGAG TTTCCCTCGG TTGAGTATGG ACCTGTTTGA CTGTCCCAAA 
GAGGTGATAC CAGCGCTGAG GAGCAGTACA CCAGGGGCCA GCCTGTCCAG CAGCCCTGCA 
CTGCTGGCCA AGAGGACCAA GCAGGAGATC AAGCTGGCCT ACAAGATGGC CAAGCGCTTC 
TACTCCGACC CTCCGCTGTG GGCCAAGTGT CTGTTCAGCC ACTGCTACAG TCTGTGGTTC 
ATCTGTCTGC CGGTGGGGGT GAGGCTGGTC CAGTCAAAGA GCTGGGCCAT GCAGCAGGCC 
TACAACGTCC TGCTCAAGAT GAGGACGTCT GAGGTGGAGG TCCTGGATGA GGTGTGTTAC 
CGTGTAGTGA TGCAGCTGTG TGGTTTGTAC GGTCTGCCGG TCATGGCCGT CCGTGTTCTG 
GTGGAGATGA AGAAGGCAGG GGTGCACCCT AACGCGATCA CCTATGGCTA CTACAACAAG 
GCTGTTCTGG AAGGCCCCTG GCCAAGTCGG AACCGCAGCG GTCTCTTCAT GTGGGCCAAG 
GTTCGCAACG TGGTGCGTAC TGTGGCCCAA TTTAAACAGG CACTCCACCG GATGCCCGCC 
ACGAAAGGAC CCACCATCAT CACAACAGTG GTGTCGGTGA GCGGCGACCT GCTGAGCCAC 
GGGAGTGCAG ACCACTCCAG TGAGGTGAAC GGAGAGGATC ACACGCTGTT CGCACGCAGC 
CTCATCATAG GAGACGCCAC GGACAACCAC TGCAGCACAG GAGGTCAGTC TGACCAGGGC 
TACGGCTCTA AAGATGAGCT GCATCAGGTG CAAGTGGATT TGGCTCCATC AGCAGCCCCT 
CTTGTCAATG TGGATCACCG CAGCAAGCCC AAAGCACAGC ACAATGATGG GGACATAGCT 
GGCTCGGTGG ACAGTTCTGT GGTCGTAGCG GAGCCCACCA GCCCTGTTGA AGCCATCCCA 
CATGTCCCCA GCATCGTCAA GCTGTCCACA GGAGGGAGCT TCGATACTGC CATGAAGAGA 
GGAGAAACTG CCCCAGGGAA GCTGTTCTCT CTGCGCAGCC AGAGCGAGAA TGTCTCTCTG 
CCGGAGGAGG GTGAGTCTAC AGCCCCGGGT GGCTCTGGGG AGGCGGGGCC TGCAGTTCAG 
GGGCAGGGCC AGCGCCAGAA GGCCTTCTCG GAACGCAGCT GCAGCTTCAG CGTCGAGAGT 
CGTGCCGGCA TGCTGATGGA GAAAAATGGT GTGGACCACA TCGCGAGACG CATGGGTGCC 
GATGCCCGAA TCCTCGCCGA TGCCCTTTCT GGGTGTGGCA CCACCCAACC ACCTACAAAT 
CCACCATCCA AGGCCCTTTT TAAGGAGTTG GAGTCAGAGG AGGGGCGTTG TCCGATGCTG 
GGGAAACTGT CAGAGGAGGA GGGTCCTGTA ATGGACCCAA CCCCATTGGA GGAGGGGCTA 
GGGAAAGAGG AGGAGAGAAT GGACGGGGAG GATGAGATGG GACTGAAGTT GGAAGAGGAA 
GGAGATTCAG GGGGGAGGGG TGATAGCTCT CTCCCAACCC TGGAGATGAA GGAGGTGGAT 
GTGGGGGCTG ATCCCCTCTC CCTGCTGATC TCAGAGCACG AAGAGTTGGC CTCCGTAACC 
AGCCAGGAGC TGCCGCACTC CCTGCCCGCC GTGGTGTCCC GAAACCTGGC AGACGAGATT 
GAGATGTACA TGAGCCTGCG AAGCCCACTG GGCCCCAAGT CCTCCAGTAT GGAGTTCCAC 
CAGGGGGGCC AGGGAGGGGA CTCTTCCCCG GACACTGCCC CAGTTAAGCA AGCTCTAGAA 
CGGAGGTCCA GCCTCCCTGT GCCCCCTGTC AAACACCCAG GGGGTTCAAG CGGGGACTCA 
ACGCCCAAAC GCAGCCCTGC TGCTGTCACG CGCTCCAAGA CATTCGCCGT AATAGCCACC 
AGGAGCCGCG GTGTTGTGGC CAGCCCGAGA TCGTCACTGA CCGCGCTGGT GAGGTCATCG 
CAAAATGGCT CGCTGGGCTC GGTCATCAAC TCCATCTCAG GCATGAAGAT GGACACCCTG 
CTCTCTGGTC CCAAGATGGA CGTGTTGAAG TCAGGCATGA AGCAGGCAGG GAACCTGGCC 
AGCAAAGTGT GGGGGGCTGT GGCCTCAGCC TATTCCTACT CCGATGACGA GGATGAGCTG 
GCCCCGGGCG ATAGGGATAG TTTCCCTGCC GGGCTGGATG AGTCCATGCT GTCAGGGGGT 
GGAGGAGGGG ACAGTCCCAC CCACAGAGGG CCAACCCAAG GCCTGGTGTC CAACGGACTG 
ACACAGAGCA GCACCAGCCT GGCCAGCAGC AGTAGCAGCA GTGACACAGG GAGAGGACCT 
CACTCCACAC TGACTCCAGG ACGGCCAGGA AGAGGTCCAG ACTCTGATCA AGGCTCCCAC 
CACACCAGCT CCTCAAGTAT CTACCAGAAC TGTGCCCTAG AGGTGCTGAT GTCCAGCTGC 
TCCCTGTGTC GGTCCTGTGA GTGTCTGGTG TACGACGAGG AGATCATGGC GGGCTGGACG 
GCAGACGACT CCAACCTCAA CACCACCTGT CCTTTCTGCC GCGCCGCCTT TCTGCCCCTG 
CTCCACGTCG AGTTCCACGA CCTGCGCACC AACACAGCGT TCTACATGAA GCCCAGTGCA 
TCAGGAGACA GTATCCACAG CGCCAGCCAC CAGCCCACAA CTACAGACTC CACCGTGCTC 
GTGGTGGGGG GTGCAGCAGA CACCCCGCGC CCGGACCTCA TCAGCTTCCC AGAATCCTCC 
TCTGAGGACC CAGGGGAGGC CCCGGCCAGC CAAACAGCCA CTCACAAGAG TTTGGTACCG 
GTACCGGAGC CGGCTCAGTC GGACCCTCTG GGCTTGCTGG AACACCAGGC AGGGAAGCGT 
GGGACTTCTC TGACGCGCAG TAACAGCGTG GGCGGGCCAC TACAGAGCCT GGTCTCTTAC 
CAGAGACAAC CCGGACACGG CGTGTCCACC ACCAGCCTGC CCTGCAGCCT GACAGAGGTG 
GATGGCCTGG GACAAAAGCG GCCCAACCCC ATGCCAGTGT CTGTGCCCTA CCTCAGCCCC 
CTGGTCCTCC GCAAAGAACT GGAGACCCTA CTAGAGAACG AGGGGGACCA GGTGATCTAC 
ACCCATAAAT TCCTGAGTCA ACACCCCATC ATCTTCTGGA ACCTGGTGTT TTATTTCTGT 
CGCCTGGACC TGCCCAGCTA CCTGCCTGGC CTCATCCTCA CCTCAGAACA CTGCAACAAT 
GGAGTACAGC TCCCCCAGAC CTCCCTGTCC CAGGACAGTA AACAGGTGTA TGTGCAGCTG 
CTGTGGGACA ACATCAACCT GCACCAGGAG CCTGGCGATC CCCTCTACCA GAGCTGGAGG 
ACCCTGCTGG AGCAGAAGGC CACCCTGGCC CCCACGGACC ATCAGGAGAC ACGGACCTTC 
CTCAACAGCA TCGTCCGCAG CATCCAGACG AACGACGTAT ACGGCCCCAT CAATCTGCTC 
CTCCGAGAGG TCAAGCGGCA CCCGGAGGTC AAACGCCAAA GGGGTATCTA TAGGGAGATC 
CTCTTCCTGG CGCTTGTGGC ATTGGGACGG GAGAACATTG ACGTCGAGGC CTTTGACCGT 
GAATACCGCC AGGCCTTTGA TGATCTGAGC ACAGAGCAGC TCAAGGCTCT GCACAAGATT 
GACCGGCCAC CCAACAGCAA TGTCCAGTGG TGCCTCAAGT GCTTTGGAGC TCCAGTCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021433801.1
CDS	404..5686
Translation	MWGSSVFLCYKKSVSVSNSINYKAGLIFRYPEDDYESFPLSESVPLFCLPMGAKIECWTPNTRYPLPVFSTFVLTNSSGEKVYGAAIQFYEAYPIDLLSEKQKVQLGLLTTVEKKMIPNRTVNTNKCICLLSRWPFFESFRKFLMFLYKLSVSGPHPLPIEKHISHFMHNVSFPSPQRPRILVQLSAHDTLMLAQPVCTPMPLSGADYSTLLINLGSENCATLLHFVLLESKILLHSLRPAVLTGVAEAVVAMIFPFQWQCPYIPLCPLSLAGVLNAPCPFIVGVDSRYFDLYDPPPDVVCVDLDTNTIYLSDEKKHSNWKNLPKKPCKSLFNSLTNLQHQLGIVRRTAQEGLAVEMTPIEADFTWHKKMTSLEMEIQEALLRFMAHILKGYRSYLKPITQAPSEKATSADSLYDLQGFLKSRERAHQKFYSQLTKTQIFIRFIEECTFVSDKDTGLAFFDDCIEKLFPSDKGNDKGTKVEGESSEDTKLMELDESQRSEHTVFVMPPEPPTTEDESDPPTRYSYKSFPRLSMDLFDCPKEVIPALRSSTPGASLSSSPALLAKRTKQEIKLAYKMAKRFYSDPPLWAKCLFSHCYSLWFICLPVGVRLVQSKSWAMQQAYNVLLKMRTSEVEVLDEVCYRVVMQLCGLYGLPVMAVRVLVEMKKAGVHPNAITYGYYNKAVLEGPWPSRNRSGLFMWAKVRNVVRTVAQFKQALHRMPATKGPTIITTVVSVSGDLLSHGSADHSSEVNGEDHTLFARSLIIGDATDNHCSTGGQSDQGYGSKDELHQVQVDLAPSAAPLVNVDHRSKPKAQHNDGDIAGSVDSSVVVAEPTSPVEAIPHVPSIVKLSTGGSFDTAMKRGETAPGKLFSLRSQSENVSLPEEGESTAPGGSGEAGPAVQGQGQRQKAFSERSCSFSVESRAGMLMEKNGVDHIARRMGADARILADALSGCGTTQPPTNPPSKALFKELESEEGRCPMLGKLSEEEGPVMDPTPLEEGLGKEEERMDGEDEMGLKLEEEGDSGGRGDSSLPTLEMKEVDVGADPLSLLISEHEELASVTSQELPHSLPAVVSRNLADEIEMYMSLRSPLGPKSSSMEFHQGGQGGDSSPDTAPVKQALERRSSLPVPPVKHPGGSSGDSTPKRSPAAVTRSKTFAVIATRSRGVVASPRSSLTALVRSSQNGSLGSVINSISGMKMDTLLSGPKMDVLKSGMKQAGNLASKVWGAVASAYSYSDDEDELAPGDRDSFPAGLDESMLSGGGGGDSPTHRGPTQGLVSNGLTQSSTSLASSSSSSDTGRGPHSTLTPGRPGRGPDSDQGSHHTSSSSIYQNCALEVLMSSCSLCRSCECLVYDEEIMAGWTADDSNLNTTCPFCRAAFLPLLHVEFHDLRTNTAFYMKPSASGDSIHSASHQPTTTDSTVLVVGGAADTPRPDLISFPESSSEDPGEAPASQTATHKSLVPVPEPAQSDPLGLLEHQAGKRGTSLTRSNSVGGPLQSLVSYQRQPGHGVSTTSLPCSLTEVDGLGQKRPNPMPVSVPYLSPLVLRKELETLLENEGDQVIYTHKFLSQHPIIFWNLVFYFCRLDLPSYLPGLILTSEHCNNGVQLPQTSLSQDSKQVYVQLLWDNINLHQEPGDPLYQSWRTLLEQKATLAPTDHQETRTFLNSIVRSIQTNDVYGPINLLLREVKRHPEVKRQRGIYREILFLALVALGRENIDVEAFDREYRQAFDDLSTEQLKALHKIDRPPNSNVQWCLKCFGAPVI

Target ORF information:

RefSeq Version	XM_021578126.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss DENN domain-containing protein 4C-like (LOC110500643), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021578126.1

ORF Insert Sequence:

ATGTGGGGCT CTAGTGTTTT CCTGTGCTAC AAGAAATCCG TGTCGGTGTC CAACTCCATT 
AATTACAAAG CCGGCCTCAT CTTCCGCTAC CCAGAGGATG ACTATGAGTC CTTCCCCCTG 
TCGGAGTCTG TGCCCCTGTT CTGCCTCCCC ATGGGGGCTA AGATCGAATG CTGGACCCCC 
AACACACGCT ACCCTCTCCC CGTCTTCTCC ACCTTTGTGT TAACCAACTC CTCTGGGGAA 
AAGGTGTACG GTGCTGCCAT CCAGTTCTAT GAGGCCTACC CCATAGATCT CCTCAGTGAG 
AAGCAGAAGG TGCAGTTGGG CCTGCTCACC ACCGTGGAGA AGAAAATGAT CCCCAACCGG 
ACAGTCAACA CCAACAAGTG CATCTGCCTC CTTTCCCGCT GGCCCTTCTT CGAGTCGTTC 
CGCAAATTCC TCATGTTCCT CTACAAGCTG TCCGTCTCGG GCCCCCACCC GCTGCCCATC 
GAAAAGCACA TCTCCCATTT CATGCACAAC GTGTCCTTCC CCTCACCTCA GAGGCCCAGA 
ATCTTGGTCC AGCTCTCTGC ACACGACACC TTGATGCTCG CACAGCCTGT GTGTACTCCT 
ATGCCTCTCA GTGGTGCAGA CTACAGTACA CTGCTGATTA ACCTGGGCTC AGAGAACTGT 
GCCACGCTGT TGCACTTTGT CCTGCTGGAG AGTAAGATCC TGCTGCACTC CTTGAGACCC 
GCTGTACTCA CCGGGGTGGC AGAGGCTGTG GTGGCGATGA TTTTTCCATT CCAGTGGCAG 
TGCCCGTACA TCCCCCTCTG CCCGCTCTCT CTGGCGGGGG TCCTCAACGC ACCCTGTCCC 
TTCATTGTGG GCGTGGACTC CCGCTACTTT GACCTCTATG ACCCTCCTCC GGACGTGGTC 
TGTGTGGACC TGGACACCAA CACGATCTAC CTGTCTGATG AGAAGAAACA CAGCAACTGG 
AAGAACCTCC CAAAGAAGCC CTGCAAGAGT CTGTTTAACT CCCTGACCAA CCTGCAACAC 
CAGCTGGGCA TCGTGCGTCG GACGGCCCAG GAGGGCTTGG CGGTGGAGAT GACCCCCATC 
GAGGCGGACT TCACCTGGCA CAAGAAGATG ACGTCCCTGG AGATGGAGAT CCAAGAGGCC 
CTGCTGCGCT TCATGGCCCA CATCCTGAAG GGCTACCGTT CCTATCTCAA ACCCATCACC 
CAGGCCCCCT CCGAGAAGGC CACCTCTGCA GACTCACTAT ATGACCTACA GGGTTTCCTA 
AAGAGCAGGG AGCGAGCCCA CCAGAAGTTC TATTCGCAGC TGACCAAGAC TCAGATCTTT 
ATACGCTTCA TCGAGGAGTG CACATTCGTC AGCGACAAGG ATACAGGCCT TGCCTTCTTC 
GACGACTGCA TTGAGAAGCT ATTTCCCTCT GATAAAGGCA ACGACAAAGG CACTAAGGTG 
GAGGGTGAGT CCTCGGAGGA CACTAAGCTC ATGGAACTAG ACGAATCACA GAGGAGTGAG 
CATACAGTCT TCGTCATGCC CCCAGAACCT CCCACCACCG AGGACGAATC TGATCCCCCT 
ACCCGCTACA GTTATAAGAG TTTCCCTCGG TTGAGTATGG ACCTGTTTGA CTGTCCCAAA 
GAGGTGATAC CAGCGCTGAG GAGCAGTACA CCAGGGGCCA GCCTGTCCAG CAGCCCTGCA 
CTGCTGGCCA AGAGGACCAA GCAGGAGATC AAGCTGGCCT ACAAGATGGC CAAGCGCTTC 
TACTCCGACC CTCCGCTGTG GGCCAAGTGT CTGTTCAGCC ACTGCTACAG TCTGTGGTTC 
ATCTGTCTGC CGGTGGGGGT GAGGCTGGTC CAGTCAAAGA GCTGGGCCAT GCAGCAGGCC 
TACAACGTCC TGCTCAAGAT GAGGACGTCT GAGGTGGAGG TCCTGGATGA GGTGTGTTAC 
CGTGTAGTGA TGCAGCTGTG TGGTTTGTAC GGTCTGCCGG TCATGGCCGT CCGTGTTCTG 
GTGGAGATGA AGAAGGCAGG GGTGCACCCT AACGCGATCA CCTATGGCTA CTACAACAAG 
GCTGTTCTGG AAGGCCCCTG GCCAAGTCGG AACCGCAGCG GTCTCTTCAT GTGGGCCAAG 
GTTCGCAACG TGGTGCGTAC TGTGGCCCAA TTTAAACAGG CACTCCACCG GATGCCCGCC 
ACGAAAGGAC CCACCATCAT CACAACAGTG GTGTCGGTGA GCGGCGACCT GCTGAGCCAC 
GGGAGTGCAG ACCACTCCAG TGAGGTGAAC GGAGAGGATC ACACGCTGTT CGCACGCAGC 
CTCATCATAG GAGACGCCAC GGACAACCAC TGCAGCACAG GAGGTCAGTC TGACCAGGGC 
TACGGCTCTA AAGATGAGCT GCATCAGGTG CAAGTGGATT TGGCTCCATC AGCAGCCCCT 
CTTGTCAATG TGGATCACCG CAGCAAGCCC AAAGCACAGC ACAATGATGG GGACATAGCT 
GGCTCGGTGG ACAGTTCTGT GGTCGTAGCG GAGCCCACCA GCCCTGTTGA AGCCATCCCA 
CATGTCCCCA GCATCGTCAA GCTGTCCACA GGAGGGAGCT TCGATACTGC CATGAAGAGA 
GGAGAAACTG CCCCAGGGAA GCTGTTCTCT CTGCGCAGCC AGAGCGAGAA TGTCTCTCTG 
CCGGAGGAGG GTGAGTCTAC AGCCCCGGGT GGCTCTGGGG AGGCGGGGCC TGCAGTTCAG 
GGGCAGGGCC AGCGCCAGAA GGCCTTCTCG GAACGCAGCT GCAGCTTCAG CGTCGAGAGT 
CGTGCCGGCA TGCTGATGGA GAAAAATGGT GTGGACCACA TCGCGAGACG CATGGGTGCC 
GATGCCCGAA TCCTCGCCGA TGCCCTTTCT GGGTGTGGCA CCACCCAACC ACCTACAAAT 
CCACCATCCA AGGCCCTTTT TAAGGAGTTG GAGTCAGAGG AGGGGCGTTG TCCGATGCTG 
GGGAAACTGT CAGAGGAGGA GGGTCCTGTA ATGGACCCAA CCCCATTGGA GGAGGGGCTA 
GGGAAAGAGG AGGAGAGAAT GGACGGGGAG GATGAGATGG GACTGAAGTT GGAAGAGGAA 
GGAGATTCAG GGGGGAGGGG TGATAGCTCT CTCCCAACCC TGGAGATGAA GGAGGTGGAT 
GTGGGGGCTG ATCCCCTCTC CCTGCTGATC TCAGAGCACG AAGAGTTGGC CTCCGTAACC 
AGCCAGGAGC TGCCGCACTC CCTGCCCGCC GTGGTGTCCC GAAACCTGGC AGACGAGATT 
GAGATGTACA TGAGCCTGCG AAGCCCACTG GGCCCCAAGT CCTCCAGTAT GGAGTTCCAC 
CAGGGGGGCC AGGGAGGGGA CTCTTCCCCG GACACTGCCC CAGTTAAGCA AGCTCTAGAA 
CGGAGGTCCA GCCTCCCTGT GCCCCCTGTC AAACACCCAG GGGGTTCAAG CGGGGACTCA 
ACGCCCAAAC GCAGCCCTGC TGCTGTCACG CGCTCCAAGA CATTCGCCGT AATAGCCACC 
AGGAGCCGCG GTGTTGTGGC CAGCCCGAGA TCGTCACTGA CCGCGCTGGT GAGGTCATCG 
CAAAATGGCT CGCTGGGCTC GGTCATCAAC TCCATCTCAG GCATGAAGAT GGACACCCTG 
CTCTCTGGTC CCAAGATGGA CGTGTTGAAG TCAGGCATGA AGCAGGCAGG GAACCTGGCC 
AGCAAAGTGT GGGGGGCTGT GGCCTCAGCC TATTCCTACT CCGATGACGA GGATGAGCTG 
GCCCCGGGCG ATAGGGATAG TTTCCCTGCC GGGCTGGATG AGTCCATGCT GTCAGGGGGT 
GGAGGAGGGG ACAGTCCCAC CCACAGAGGG CCAACCCAAG GCCTGGTGTC CAACGGACTG 
ACACAGAGCA GCACCAGCCT GGCCAGCAGC AGTAGCAGCA GTGACACAGG GAGAGGACCT 
CACTCCACAC TGACTCCAGG ACGGCCAGGA AGAGGTCCAG ACTCTGATCA AGGCTCCCAC 
CACACCAGCT CCTCAAGTAT CTACCAGAAC TGTGCCCTAG AGGTGCTGAT GTCCAGCTGC 
TCCCTGTGTC GGTCCTGTGA GTGTCTGGTG TACGACGAGG AGATCATGGC GGGCTGGACG 
GCAGACGACT CCAACCTCAA CACCACCTGT CCTTTCTGCC GCGCCGCCTT TCTGCCCCTG 
CTCCACGTCG AGTTCCACGA CCTGCGCACC AACACAGCGT TCTACATGAA GCCCAGTGCA 
TCAGGAGACA GTATCCACAG CGCCAGCCAC CAGCCCACAA CTACAGACTC CACCGTGCTC 
GTGGTGGGGG GTGCAGCAGA CACCCCGCGC CCGGACCTCA TCAGCTTCCC AGAATCCTCC 
TCTGAGGACC CAGGGGAGGC CCCGGCCAGC CAAACAGCCA CTCACAAGAG TTTGGTACCG 
GTACCGGAGC CGGCTCAGTC GGACCCTCTG GGCTTGCTGG AACACCAGGC AGGGAAGCGT 
GGGACTTCTC TGACGCGCAG TAACAGCGTG GGCGGGCCAC TACAGAGCCT GGTCTCTTAC 
CAGAGACAAC CCGGACACGG CGTGTCCACC ACCAGCCTGC CCTGCAGCCT GACAGAGGTG 
GATGGCCTGG GACAAAAGCG GCCCAACCCC ATGCCAGTGT CTGTGCCCTA CCTCAGCCCC 
CTGGTCCTCC GCAAAGAACT GGAGACCCTA CTAGAGAACG AGGGGGACCA GGTGATCTAC 
ACCCATAAAT TCCTGAGTCA ACACCCCATC ATCTTCTGGA ACCTGGTGTT TTATTTCTGT 
CGCCTGGACC TGCCCAGCTA CCTGCCTGGC CTCATCCTCA CCTCAGAACA CTGCAACAAT 
GGAGTACAGC TCCCCCAGAC CTCCCTGTCC CAGGACAGTA AACAGGTGTA TGTGCAGCTG 
CTGTGGGACA ACATCAACCT GCACCAGGAG CCTGGCGATC CCCTCTACCA GAGCTGGAGG 
ACCCTGCTGG AGCAGAAGGC CACCCTGGCC CCCACGGACC ATCAGGAGAC ACGGACCTTC 
CTCAACAGCA TCGTCCGCAG CATCCAGACG AACGACGTAT ACGGCCCCAT CAATCTGCTC 
CTCCGAGAGG TCAAGCGGCA CCCGGAGGTC AAACGCCAAA GGGGTATCTA TAGGGAGATC 
CTCTTCCTGG CGCTTGTGGC ATTGGGACGG GAGAACATTG ACGTCGAGGC CTTTGACCGT 
GAATACCGCC AGGCCTTTGA TGATCTGAGC ACAGAGCAGC TCAAGGCTCT GCACAAGATT 
GACCGGCCAC CCAACAGCAA TGTCCAGTGG TGCCTCAAGT GCTTTGGAGC TCCAGTCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC110500643 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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