prpf8 cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene
Clones
gRNAs

Summary

Gene Symbol	prpf8
Entrez Gene ID	103045815
Full Name	pre-mRNA processing factor 8
Gene Type	protein-coding
Organism	Astyanax mexicanus(Mexican tetra)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007260888.2	XP_007260950.1	pre-mRNA-processing-splicing factor 8
XM_007260888.3	XP_007260950.2	pre-mRNA-processing-splicing factor 8

Pathways from BioSystems
Gene Ontology

The following prpf8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prpf8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAs114716	XM_007260888.3 Latest version!	Astyanax mexicanus pre-mRNA processing factor 8 (prpf8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAs42413	XM_007260888.2 Latest version!	Astyanax mexicanus pre-mRNA processing factor 8 (prpf8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prpf8 ( XM_007260888.3 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007260950.2 Astyanax mexicanus pre-mRNA processing factor 8 (prpf8), mRNA.

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CloneID	OAs114716
Clone ID Related Accession (Same CDS sequence)	XM_007260888.3
Accession Version	XM_007260888.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035897.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 3, 2017 this sequence version replaced XM_007260888.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTA CATTCCCCTA CAGAGGTGTC CCTGGTGTTC CTCCTCCTGC CCCGGTGCCA 
GACTACATGT CTGAGGAGAA GCTTCAAGAA AAAGCCCGGA AATGGCAGCA GCTGCAGGCA 
AAACGCTACT CGGAGAAGAG GAAATTTGGT TTTGTCGATG CTCAGAAAGA GGACATGCCA 
CCAGAGCATG TGCGGAAAAT CATTCGGGAC CATGGAGATA TGACCAACAG GAAATTTCGT 
CACGACAAAC GAGTCTATCT TGGGGCTCTG AAGTACATGC CTCATGCAGT ACTGAAACTG 
CTGGAGAATA TGCCCATGCC CTGGGAGCAG ATCAGAGATG TGCCAGTGCT GTACCACATC 
ACAGGAGCCA TTTCCTTTGT AAATGAAATT CCTTGGGTCA TTGAACCAGT TTACATTGCA 
CAATGGGGTA CCATGTGGAT CATGATGCGT CGTGAGAAAA GAGACAGAAG GCACTTCAAG 
CGTATGCGCT TTCCTCCCTT TGATGATGAG GAGCCCCCAC TGGACTATGC TGATAACATT 
CTGGATGTGG AGCCCCTGGA AGCCATTCAG ATGGAAATGG ACCCTGAGGA AGATTGCTCA 
GTTGTAGAAT GGTTTTACGA ACACCAGCCA CTGAAGGATG CTACCAAATA TGTCAATGGG 
ACGACATACA GACGTTGGCA GTTCACCTTG CCAATGATGT CCACTCTTTA TCGGTTAGCC 
AACCAGCTGC TAACTGATTT AGTGGATAGG AACTACTTCT ATTTGTTTGA CCTGAAGGCC 
TTCTTCACTT CAAAGGCCTT AAACATGGCC ATTCCTGGAG GGCCCAAATT TGAACCCCTG 
GTCAGAGACA TCAATCTACA GGATGAAGAC TGGAATGAGT TTAACGACAT CAACAAGATC 
ATCATCAGAC AGCCGATTAG AACAGAGTAC AAGATTGCTT TCCCATACTT GTACAATAAT 
CTTCCACATC ACGTCCATCT CACTTGGTAC CACACTCCTA ATGTGGTGTT CATCAAGACA 
GAGGATCCTG ATTTGCCTGC TTTCTACTTT GATCCTCTCA TCAATCCCAT CTCTCACAGA 
CATTCTGTAA AAAGCCAAGA GCCACTGCCA GACGATGACG AGGAATTTGA GCTTCCTGAG 
TATGTGGAGC CTTTCCTCAA AGAGACGCCT CTTTATACTG ACAACACAGC CAATGGGATT 
GCCCTTCTCT GGGCCCCACG TCCGTTCAAC CTGCGCTCCG GAAGGTCCAG GCGTGCCATC 
GATGTTCCCC TGATCAAGAA TTGGTACAGG GAACACTGCC CTGCTGGACA GCCTGTGAAA 
GTACGTGTGT CCTACCAGAA GCTCCTGAAG TACTATGTTC TCAATGCGCT GAAACACAGA 
CCACCTAAAG CGCAGAAGAA GAGATACCTT TTCCGATCCT TCAAGGCCAC AAAATTCTTC 
CAGTCCACAA AGCTAGACTG GGTGGAGGTG GGCCTGCAGG TTTGCCGACA AGGTTACAAC 
ATGCTGAACC TGCTGATTCA CAGAAAGAAC CTCAACTACT TGCACTTGGA CTACAATTTC 
AACTTGAAGC CTGTCAAAAC CCTCACCACA AAAGAGCGTA AAAAGTCCCG ATTCGGGAAT 
GCGTTCCACT TGTGCCGTGA GGTTCTCCGT CTGAGCAAGT TGGTAGTGGA CAGTCATGTA 
CAGTACAGAC TGGGAAATGT TGATGCATTC CAGTTGGCAG ATGGGCTGCA GTACATCTTT 
GCCCATGTGG GTCAGCTGAC TGGCATGTAC CGTTACAAGT ACAAGTTGAT GAGGCAGATC 
AGGATGTGCA AAGACTTGAA GCATCTCATC TACTACCGCT TCAATACAGG TCCAGTTGGG 
AAGGGTCCAG GTTGTGGTTT CTGGGCTCCA GGATGGAGAG TTTGGCTCTT CTTTATGAGA 
GGCATTACTC CTCTTCTGGA GAGATGGCTT GGCAACCTGC TGGCCAGGCA GTTCGAAGGT 
CGTCATTCCA AGGGAGTTGC AAAAACTGTC ACCAAGCAGC GTGTGGAGTC TCACTTTGAT 
CTTGAGCTGA GAGCTGCTGT GATGCATGAC ATCCTGGACA TGATGCCTGA AGGCATCAAA 
CAGAACAAGG CAAGGACCAT CCTGCAGCAC CTCAGTGAGT CCTGGAGATG CTGGAAGGCT 
AACATTCCTT GGAAGGTCCC AGGACTTCCA ACCCCCATCG AGAACATGAT CTTGCGCTAT 
GTGAAGGCCA AAGCTGATTG GTGGACCAAC ACAGCCCATT ACAACAGAGA GCGAATTAGG 
AGAGGTGCTA CTGTGGACAA GACTGTCTGC AAGAAAAACC TTGGCCGCCT CACACGTCTG 
TACCTGAAGG CTGAGCAGGA GAGGCAGCAC AACTACTTGA AGGATGGTCC ATACATCACT 
GCAGAAGAGG CGGTGGCCAT CTACACCACT ACTGTCCATT GGCTGGAGAG TCGGCGCTTC 
TCTCCAATTC CATTCCCTCC TCTCTCCTAC AAACACGACA CCAAGCTTCT CATCCTGGCT 
CTGGAGAGGC TCAAAGAAGC CTACAGTGTG AAGTCAAGGT TGAATCAGTC TCAGCGTGAG 
GAGTTGGGTC TGATCGAGCA GGCGTATGAC AACCCTCACG AGGCTCTGTC CAGGATCAAG 
CGTCACCTCC TCACCCAGAG AGCTTTCAAA GAGGTGGGCA TTGAGTTCAT GGACCTTTAT 
AGCCACTTGG TACCGGTGTA TGATGTGGAG CCCCTGGAGA AGATCACTGA TGCATACCTG 
GATCAGTACT TGTGGTACGA GGCTGACAAG CGCAGGCTTT TCCCACCGTG GATCAAACCA 
GCCGACACAG AGCCACCTCC ACTTTTGGTA TACAAATGGT GCCAAGGAAT CAACAATCTG 
CAGGATGTGT GGGAAACAAC CGAGGGAGAA TGCAACGTCA TGCTGGAGTC ACGCTATGAG 
AAAATGTATG AAAAGATCGA CTTGACGCTG CTCAACAGGC TGCTGCGTCT CATTGTTGAC 
CACAACATTG CTGATTATAT GACTGCCAAG AACAACGTTG TCATCAACTA CAAGGACATG 
AACCACACCA ACTCGTACGG CATCATTCGT GGCCTGCAGT TTGCTTCCTT CATTGTCCAG 
TACTATGGGC TTGTGATGGA CCTTCTTGTT CTGGGTCTTC ATCGGGCCAG TGAAATGGCA 
GGACCTCCCC AGATGCCCAA TGACTTCCTC AGCTTTCAGG ACAGTGCTAC AGAGGGTGCA 
CATCCTATTC GCCTTTACTG CAGATACATC GATCGCATCC ACATGTTCTT CAGGTTTTCT 
GCTGATGAGG CCAGAGATCT GATTCAGCGG TACCTAACTG AACACCCAGA CCCCAACAAT 
GAAAACATTG TGGGCTACAA CAATAAGAAA TGCTGGCCTA GAGATGCCAG GATGAGGTTG 
ATGAAGCATG ATGTTAACCT GGGTCGCGCT GTGTTCTGGG ACATTAAGAA TCGCCTTCCT 
CGCTCTGTGA CCACGGTGCA GTGGGAGAAC AGCTTTGTCT CAGTCTACAG TAAAGACAAT 
CCCAACCTGC TCTTTAACAT GTGTGGCTTT GAGTGTCGTA TCCTGCCAAA GTGCCGCACC 
AGTTATGAGG AGTTCACCCA CAAAGATGGT GTGTGGAACC TGCAGAATGA GGTGACCAAG 
GAAAGGACAG CTCAGTGCTT CCTGCGAGTG GATGATGAAT CTATGCAGAG GTTTCACAAC 
CGAGTGCGAC AGATTCTCAT GGCTTCTGGA TCTACCACTT TCACCAAGAT CGTGAACAAA 
TGGAACACAG CTTTGATTGG TCTGATGACC TACTTCCGTG AGGCTGTGGT CAACACTCAG 
GAGTTGCTGG ACCTTCTAGT GAAGTGCGAG AATAAGATCC AGACCCGTAT TAAGATCGGT 
CTGAACTCTA AAATGCCTAG CCGTTTCCCT CCTGTGGTCT TCTACACCCC CAAGGAGCTG 
GGTGGACTGG GCATGTTGTC CATGGGCCAT GTGCTCATCC CACAGTCTGA CCTGAGGTGG 
TCCAAACAAA CGGATGTAGG AATCACTCAT TTCCGCTCTG GTATGAGTCA CGAAGAGGAT 
CAGCTGATTC CCAATCTCTA TCGCTACATT CAGCCTTGGG AGAGTGAGTT CATTGACTCT 
CAGAGAGTCT GGGCTGAATA TGCCCTGAAG AGACAGGAGG CCATTGCCCA GAACAGGCGC 
TTGACTCTTG AGGATTTGGA GGATTCATGG GACAGAGGTA TTCCTCGTAT CAACACCCTC 
TTCCAAAAGG ACAGGCACAC ACTGGCCTAT GACAAGGGCT GGAGAGTCAG AACAGATTTC 
AAACAGTACC AGGTGCTGAA GCAAAACCCA TTCTGGTGGA CACATCAGAG GCACGACGGG 
AAACTGTGGA ATCTGAACAA TTACCGCACA GATATGATCC AGGCTTTGGG AGGAGTGGAG 
GGAATTTTGG AGCACACCCT CTTCAAGGGC ACATACTTTC CAACATGGGA AGGTCTCTTC 
TGGGAAAAGG CCAGTGGCTT TGAGGAGTCC ATGAAATGGA AGAAACTGAC CAACGCTCAG 
AGATCTGGTC TGAACCAGAT CCCCAATCGA CGATTCACTC TGTGGTGGTC TCCCACTATC 
AACAGAGCTA ATGTCTATGT GGGCTTCCAG GTGCAGTTGG ATCTCACTGG AATCTTCATG 
CATGGCAAAA TTCCAACACT CAAGATCTCC TTGATTCAGA TCTTCAGGGC TCACTTGTGG 
CAAAAGATTC ATGAGAGCAT TGTCATGGAT CTTTGTCAGG TGTTTGACCA AGAGTTGGAT 
GCTCTGGAGA TTGAGACTGT CCAGAAGGAA ACCATCCATC CCAGGAAGTC GTACAAAATG 
AACTCCTCCT GTGCAGACAT TCTGCTGTTT GCCTCCTACA AGTGGAATGT CTCCCGACCA 
TCACTGCTTG CTGACTCAAA GGATGTGATG GACAGTACCA CCACTCAGAA GTACTGGATT 
GACATTCAGC TCAGATGGGG AGATTATGAC TCCCATGACA TTGAAAGATA TGCTAGAGCC 
AAGTTCCTGG ACTACACCAC AGATAACATG AGTATCTACC CATCGCCCAC TGGTGTGCTG 
ATTGCCATTG ATTTGGCCTA CAATCTGCAC AGTGCCTATG GAAACTGGTT CCCTGGCAGC 
AAGCCTCTGA TCCAACAGGC CATGGCTAAG ATCATGAAGG CCAATCCTGC CCTTTATGTG 
CTAAGGGAGC GCATCCGCAA GGGTCTGCAG CTTTACTCCT CAGAGCCCAC TGAGCCCTAC 
CTGTCCTCCC AGAATTATGG AGAGCTCTTC TCTAACCAGA TCATCTGGTT TGTGGATGAC 
ACTAATGTGT ACCGTGTCAC CATCCACAAG ACCTTTGAGG GCAACTTGAC AACGAAACCA 
ATCAATGGAG CCATTTTCAT CTTCAATCCA AGAACTGGAC AGCTCTTCCT GAAGATCATT 
CACACTTCTG TGTGGGCTGG ACAGAAGCGT CTGGGACAGC TGGCCAAGTG GAAGACTGCT 
GAGGAAGTTG CTGCTCTTAT TCGATCACTG CCTGTTGAAG AGCAGCCCAA GCAGATCATT 
GTCACCAGAA AGGGCATGTT GGATCCTCTT GAGGTGCACT TGTTGGATTT CCCTAACATT 
GTGATCAAGG GATCCGAGCT ACAGCTGCCC TTCCAGGCCT GTCTGAAGGT GGAGAAGTTT 
GGAGACCTTA TTCTGAAGGC CACTGAGCCT CAGATGGTTC TGTTCAATCT GTATGATGAC 
TGGCTCAAGA CGATCTCGTC TTACACAGCT TTCTCACGTC TGATCTTGAT CCTGAGAGCT 
CTGCATGTGA ACAATGACCG TGCAAAGGTG ATCCTGAAGC CAGACAAAAC CACCATCACC 
GAGCCACACC ATATCTGGCC CACTTTAACT GACGAGGAGT GGATCAAGGT GGAGGTGCAG 
CTCAAAGATC TCATTCTGGC TGACTATGGC AAGAAGAACA ACGTGAACGT GGCTTCTTTG 
ACGCAGTCTG AGATCAGGGA CATCATCCTG GGTATGGAGA TTTCAGCTCC TTCCCAGCAG 
AGACAGCAGA TTGCTGAGAT AGAGAAGCAG ACCAAAGAGC AGTCTCAGTT AACAGCCACC 
CAGACCCGCA CCGTGAACAA GCACGGTGAT GAGATCATCA CTTCCACTAC CAGCAATTAC 
GAGACACAAA CATTTTCCTC CAAGACTGAA TGGAGGGTCA GGGCCATCTC TGCAGCCAAC 
TTGCACTTGC GAACCAATCA CATCTACGTG TCTTCAGATG ACATTAAGGA AACTGGATAC 
ACTTACATTC TGCCCAAGAA TGTGCTGAAG AAGTTCATCT GCATCTCCGA CTTGCGTGCT 
CAAATTGCAG GCTATCTGTA TGGCACTAGT CCTCCAGATA ATCCCCAAGT GAAGGAGATT 
CGCTGCATTG TGATGGTGCC TCAGTGGGGA ACACACCAGA CTGTTCATCT GCCCAATCAG 
CTGCCACAGC ATGAATATCT AAAGGAAATG GAGCCACTTG GCTGGATCCA CACCCAGCCC 
AATGAATCTC CTCAGCTTTC TCCTCAGGAT GTGACCACAC ATGCCAAGGT CATGGCAGAC 
AACCCATCCT GGGATGGGGA GAAAACCATC ATTATTACCT GCAGTTTCAC CCCAGGATCC 
TGTACCCTTA CTGCTTATAA ACTGACTCCT AGTGGATATG AATGGGGCAG GCAGAACACA 
GACAAGGGCA ACAACCCTAA GGGTTACCTG CCATCCCATT ACGAGAGGGT GCAGATGCTG 
CTGTCTGATC GCTTCCTTGG TTTCTTCATG GTACCTGGGC AAGTGTCCTG GAACTACAAC 
TTCATGGGTG TCCGTCATGA CCCCAACATG AAGTACGACC TGCAGCTGTC CAACCCTAAA 
GAGTTCTATC ATGAGGTTCA CAGGCCCTCT CACTTCCTCA ACTTTGCCTC TCTGCAAGAG 
GGTGAAATCT ACAACGCCGA CCGTGAGGAT ATGTATGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007260950.2
CDS	118..7119
Translation	MAATFPYRGVPGVPPPAPVPDYMSEEKLQEKARKWQQLQAKRYSEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYIAQWGTMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQMEMDPEEDCSVVEWFYEHQPLKDATKYVNGTTYRRWQFTLPMMSTLYRLANQLLTDLVDRNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEYVEPFLKETPLYTDNTANGIALLWAPRPFNLRSGRSRRAIDVPLIKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLSKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAPGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSESWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAIYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETTEGECNVMLESRYEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDSATEGAHPIRLYCRYIDRIHMFFRFSADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGTSPPDNPQVKEIRCIVMVPQWGTHQTVHLPNQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKVMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPGQVSWNYNFMGVRHDPNMKYDLQLSNPKEFYHEVHRPSHFLNFASLQEGEIYNADREDMYA

Target ORF information:

RefSeq Version	XM_007260888.3
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus pre-mRNA processing factor 8 (prpf8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007260888.3

ORF Insert Sequence:

ATGGCAGCTA CATTCCCCTA CAGAGGTGTC CCTGGTGTTC CTCCTCCTGC CCCGGTGCCA 
GACTACATGT CTGAGGAGAA GCTTCAAGAA AAAGCCCGGA AATGGCAGCA GCTGCAGGCA 
AAACGCTACT CGGAGAAGAG GAAATTTGGT TTTGTCGATG CTCAGAAAGA GGACATGCCA 
CCAGAGCATG TGCGGAAAAT CATTCGGGAC CATGGAGATA TGACCAACAG GAAATTTCGT 
CACGACAAAC GAGTCTATCT TGGGGCTCTG AAGTACATGC CTCATGCAGT ACTGAAACTG 
CTGGAGAATA TGCCCATGCC CTGGGAGCAG ATCAGAGATG TGCCAGTGCT GTACCACATC 
ACAGGAGCCA TTTCCTTTGT AAATGAAATT CCTTGGGTCA TTGAACCAGT TTACATTGCA 
CAATGGGGTA CCATGTGGAT CATGATGCGT CGTGAGAAAA GAGACAGAAG GCACTTCAAG 
CGTATGCGCT TTCCTCCCTT TGATGATGAG GAGCCCCCAC TGGACTATGC TGATAACATT 
CTGGATGTGG AGCCCCTGGA AGCCATTCAG ATGGAAATGG ACCCTGAGGA AGATTGCTCA 
GTTGTAGAAT GGTTTTACGA ACACCAGCCA CTGAAGGATG CTACCAAATA TGTCAATGGG 
ACGACATACA GACGTTGGCA GTTCACCTTG CCAATGATGT CCACTCTTTA TCGGTTAGCC 
AACCAGCTGC TAACTGATTT AGTGGATAGG AACTACTTCT ATTTGTTTGA CCTGAAGGCC 
TTCTTCACTT CAAAGGCCTT AAACATGGCC ATTCCTGGAG GGCCCAAATT TGAACCCCTG 
GTCAGAGACA TCAATCTACA GGATGAAGAC TGGAATGAGT TTAACGACAT CAACAAGATC 
ATCATCAGAC AGCCGATTAG AACAGAGTAC AAGATTGCTT TCCCATACTT GTACAATAAT 
CTTCCACATC ACGTCCATCT CACTTGGTAC CACACTCCTA ATGTGGTGTT CATCAAGACA 
GAGGATCCTG ATTTGCCTGC TTTCTACTTT GATCCTCTCA TCAATCCCAT CTCTCACAGA 
CATTCTGTAA AAAGCCAAGA GCCACTGCCA GACGATGACG AGGAATTTGA GCTTCCTGAG 
TATGTGGAGC CTTTCCTCAA AGAGACGCCT CTTTATACTG ACAACACAGC CAATGGGATT 
GCCCTTCTCT GGGCCCCACG TCCGTTCAAC CTGCGCTCCG GAAGGTCCAG GCGTGCCATC 
GATGTTCCCC TGATCAAGAA TTGGTACAGG GAACACTGCC CTGCTGGACA GCCTGTGAAA 
GTACGTGTGT CCTACCAGAA GCTCCTGAAG TACTATGTTC TCAATGCGCT GAAACACAGA 
CCACCTAAAG CGCAGAAGAA GAGATACCTT TTCCGATCCT TCAAGGCCAC AAAATTCTTC 
CAGTCCACAA AGCTAGACTG GGTGGAGGTG GGCCTGCAGG TTTGCCGACA AGGTTACAAC 
ATGCTGAACC TGCTGATTCA CAGAAAGAAC CTCAACTACT TGCACTTGGA CTACAATTTC 
AACTTGAAGC CTGTCAAAAC CCTCACCACA AAAGAGCGTA AAAAGTCCCG ATTCGGGAAT 
GCGTTCCACT TGTGCCGTGA GGTTCTCCGT CTGAGCAAGT TGGTAGTGGA CAGTCATGTA 
CAGTACAGAC TGGGAAATGT TGATGCATTC CAGTTGGCAG ATGGGCTGCA GTACATCTTT 
GCCCATGTGG GTCAGCTGAC TGGCATGTAC CGTTACAAGT ACAAGTTGAT GAGGCAGATC 
AGGATGTGCA AAGACTTGAA GCATCTCATC TACTACCGCT TCAATACAGG TCCAGTTGGG 
AAGGGTCCAG GTTGTGGTTT CTGGGCTCCA GGATGGAGAG TTTGGCTCTT CTTTATGAGA 
GGCATTACTC CTCTTCTGGA GAGATGGCTT GGCAACCTGC TGGCCAGGCA GTTCGAAGGT 
CGTCATTCCA AGGGAGTTGC AAAAACTGTC ACCAAGCAGC GTGTGGAGTC TCACTTTGAT 
CTTGAGCTGA GAGCTGCTGT GATGCATGAC ATCCTGGACA TGATGCCTGA AGGCATCAAA 
CAGAACAAGG CAAGGACCAT CCTGCAGCAC CTCAGTGAGT CCTGGAGATG CTGGAAGGCT 
AACATTCCTT GGAAGGTCCC AGGACTTCCA ACCCCCATCG AGAACATGAT CTTGCGCTAT 
GTGAAGGCCA AAGCTGATTG GTGGACCAAC ACAGCCCATT ACAACAGAGA GCGAATTAGG 
AGAGGTGCTA CTGTGGACAA GACTGTCTGC AAGAAAAACC TTGGCCGCCT CACACGTCTG 
TACCTGAAGG CTGAGCAGGA GAGGCAGCAC AACTACTTGA AGGATGGTCC ATACATCACT 
GCAGAAGAGG CGGTGGCCAT CTACACCACT ACTGTCCATT GGCTGGAGAG TCGGCGCTTC 
TCTCCAATTC CATTCCCTCC TCTCTCCTAC AAACACGACA CCAAGCTTCT CATCCTGGCT 
CTGGAGAGGC TCAAAGAAGC CTACAGTGTG AAGTCAAGGT TGAATCAGTC TCAGCGTGAG 
GAGTTGGGTC TGATCGAGCA GGCGTATGAC AACCCTCACG AGGCTCTGTC CAGGATCAAG 
CGTCACCTCC TCACCCAGAG AGCTTTCAAA GAGGTGGGCA TTGAGTTCAT GGACCTTTAT 
AGCCACTTGG TACCGGTGTA TGATGTGGAG CCCCTGGAGA AGATCACTGA TGCATACCTG 
GATCAGTACT TGTGGTACGA GGCTGACAAG CGCAGGCTTT TCCCACCGTG GATCAAACCA 
GCCGACACAG AGCCACCTCC ACTTTTGGTA TACAAATGGT GCCAAGGAAT CAACAATCTG 
CAGGATGTGT GGGAAACAAC CGAGGGAGAA TGCAACGTCA TGCTGGAGTC ACGCTATGAG 
AAAATGTATG AAAAGATCGA CTTGACGCTG CTCAACAGGC TGCTGCGTCT CATTGTTGAC 
CACAACATTG CTGATTATAT GACTGCCAAG AACAACGTTG TCATCAACTA CAAGGACATG 
AACCACACCA ACTCGTACGG CATCATTCGT GGCCTGCAGT TTGCTTCCTT CATTGTCCAG 
TACTATGGGC TTGTGATGGA CCTTCTTGTT CTGGGTCTTC ATCGGGCCAG TGAAATGGCA 
GGACCTCCCC AGATGCCCAA TGACTTCCTC AGCTTTCAGG ACAGTGCTAC AGAGGGTGCA 
CATCCTATTC GCCTTTACTG CAGATACATC GATCGCATCC ACATGTTCTT CAGGTTTTCT 
GCTGATGAGG CCAGAGATCT GATTCAGCGG TACCTAACTG AACACCCAGA CCCCAACAAT 
GAAAACATTG TGGGCTACAA CAATAAGAAA TGCTGGCCTA GAGATGCCAG GATGAGGTTG 
ATGAAGCATG ATGTTAACCT GGGTCGCGCT GTGTTCTGGG ACATTAAGAA TCGCCTTCCT 
CGCTCTGTGA CCACGGTGCA GTGGGAGAAC AGCTTTGTCT CAGTCTACAG TAAAGACAAT 
CCCAACCTGC TCTTTAACAT GTGTGGCTTT GAGTGTCGTA TCCTGCCAAA GTGCCGCACC 
AGTTATGAGG AGTTCACCCA CAAAGATGGT GTGTGGAACC TGCAGAATGA GGTGACCAAG 
GAAAGGACAG CTCAGTGCTT CCTGCGAGTG GATGATGAAT CTATGCAGAG GTTTCACAAC 
CGAGTGCGAC AGATTCTCAT GGCTTCTGGA TCTACCACTT TCACCAAGAT CGTGAACAAA 
TGGAACACAG CTTTGATTGG TCTGATGACC TACTTCCGTG AGGCTGTGGT CAACACTCAG 
GAGTTGCTGG ACCTTCTAGT GAAGTGCGAG AATAAGATCC AGACCCGTAT TAAGATCGGT 
CTGAACTCTA AAATGCCTAG CCGTTTCCCT CCTGTGGTCT TCTACACCCC CAAGGAGCTG 
GGTGGACTGG GCATGTTGTC CATGGGCCAT GTGCTCATCC CACAGTCTGA CCTGAGGTGG 
TCCAAACAAA CGGATGTAGG AATCACTCAT TTCCGCTCTG GTATGAGTCA CGAAGAGGAT 
CAGCTGATTC CCAATCTCTA TCGCTACATT CAGCCTTGGG AGAGTGAGTT CATTGACTCT 
CAGAGAGTCT GGGCTGAATA TGCCCTGAAG AGACAGGAGG CCATTGCCCA GAACAGGCGC 
TTGACTCTTG AGGATTTGGA GGATTCATGG GACAGAGGTA TTCCTCGTAT CAACACCCTC 
TTCCAAAAGG ACAGGCACAC ACTGGCCTAT GACAAGGGCT GGAGAGTCAG AACAGATTTC 
AAACAGTACC AGGTGCTGAA GCAAAACCCA TTCTGGTGGA CACATCAGAG GCACGACGGG 
AAACTGTGGA ATCTGAACAA TTACCGCACA GATATGATCC AGGCTTTGGG AGGAGTGGAG 
GGAATTTTGG AGCACACCCT CTTCAAGGGC ACATACTTTC CAACATGGGA AGGTCTCTTC 
TGGGAAAAGG CCAGTGGCTT TGAGGAGTCC ATGAAATGGA AGAAACTGAC CAACGCTCAG 
AGATCTGGTC TGAACCAGAT CCCCAATCGA CGATTCACTC TGTGGTGGTC TCCCACTATC 
AACAGAGCTA ATGTCTATGT GGGCTTCCAG GTGCAGTTGG ATCTCACTGG AATCTTCATG 
CATGGCAAAA TTCCAACACT CAAGATCTCC TTGATTCAGA TCTTCAGGGC TCACTTGTGG 
CAAAAGATTC ATGAGAGCAT TGTCATGGAT CTTTGTCAGG TGTTTGACCA AGAGTTGGAT 
GCTCTGGAGA TTGAGACTGT CCAGAAGGAA ACCATCCATC CCAGGAAGTC GTACAAAATG 
AACTCCTCCT GTGCAGACAT TCTGCTGTTT GCCTCCTACA AGTGGAATGT CTCCCGACCA 
TCACTGCTTG CTGACTCAAA GGATGTGATG GACAGTACCA CCACTCAGAA GTACTGGATT 
GACATTCAGC TCAGATGGGG AGATTATGAC TCCCATGACA TTGAAAGATA TGCTAGAGCC 
AAGTTCCTGG ACTACACCAC AGATAACATG AGTATCTACC CATCGCCCAC TGGTGTGCTG 
ATTGCCATTG ATTTGGCCTA CAATCTGCAC AGTGCCTATG GAAACTGGTT CCCTGGCAGC 
AAGCCTCTGA TCCAACAGGC CATGGCTAAG ATCATGAAGG CCAATCCTGC CCTTTATGTG 
CTAAGGGAGC GCATCCGCAA GGGTCTGCAG CTTTACTCCT CAGAGCCCAC TGAGCCCTAC 
CTGTCCTCCC AGAATTATGG AGAGCTCTTC TCTAACCAGA TCATCTGGTT TGTGGATGAC 
ACTAATGTGT ACCGTGTCAC CATCCACAAG ACCTTTGAGG GCAACTTGAC AACGAAACCA 
ATCAATGGAG CCATTTTCAT CTTCAATCCA AGAACTGGAC AGCTCTTCCT GAAGATCATT 
CACACTTCTG TGTGGGCTGG ACAGAAGCGT CTGGGACAGC TGGCCAAGTG GAAGACTGCT 
GAGGAAGTTG CTGCTCTTAT TCGATCACTG CCTGTTGAAG AGCAGCCCAA GCAGATCATT 
GTCACCAGAA AGGGCATGTT GGATCCTCTT GAGGTGCACT TGTTGGATTT CCCTAACATT 
GTGATCAAGG GATCCGAGCT ACAGCTGCCC TTCCAGGCCT GTCTGAAGGT GGAGAAGTTT 
GGAGACCTTA TTCTGAAGGC CACTGAGCCT CAGATGGTTC TGTTCAATCT GTATGATGAC 
TGGCTCAAGA CGATCTCGTC TTACACAGCT TTCTCACGTC TGATCTTGAT CCTGAGAGCT 
CTGCATGTGA ACAATGACCG TGCAAAGGTG ATCCTGAAGC CAGACAAAAC CACCATCACC 
GAGCCACACC ATATCTGGCC CACTTTAACT GACGAGGAGT GGATCAAGGT GGAGGTGCAG 
CTCAAAGATC TCATTCTGGC TGACTATGGC AAGAAGAACA ACGTGAACGT GGCTTCTTTG 
ACGCAGTCTG AGATCAGGGA CATCATCCTG GGTATGGAGA TTTCAGCTCC TTCCCAGCAG 
AGACAGCAGA TTGCTGAGAT AGAGAAGCAG ACCAAAGAGC AGTCTCAGTT AACAGCCACC 
CAGACCCGCA CCGTGAACAA GCACGGTGAT GAGATCATCA CTTCCACTAC CAGCAATTAC 
GAGACACAAA CATTTTCCTC CAAGACTGAA TGGAGGGTCA GGGCCATCTC TGCAGCCAAC 
TTGCACTTGC GAACCAATCA CATCTACGTG TCTTCAGATG ACATTAAGGA AACTGGATAC 
ACTTACATTC TGCCCAAGAA TGTGCTGAAG AAGTTCATCT GCATCTCCGA CTTGCGTGCT 
CAAATTGCAG GCTATCTGTA TGGCACTAGT CCTCCAGATA ATCCCCAAGT GAAGGAGATT 
CGCTGCATTG TGATGGTGCC TCAGTGGGGA ACACACCAGA CTGTTCATCT GCCCAATCAG 
CTGCCACAGC ATGAATATCT AAAGGAAATG GAGCCACTTG GCTGGATCCA CACCCAGCCC 
AATGAATCTC CTCAGCTTTC TCCTCAGGAT GTGACCACAC ATGCCAAGGT CATGGCAGAC 
AACCCATCCT GGGATGGGGA GAAAACCATC ATTATTACCT GCAGTTTCAC CCCAGGATCC 
TGTACCCTTA CTGCTTATAA ACTGACTCCT AGTGGATATG AATGGGGCAG GCAGAACACA 
GACAAGGGCA ACAACCCTAA GGGTTACCTG CCATCCCATT ACGAGAGGGT GCAGATGCTG 
CTGTCTGATC GCTTCCTTGG TTTCTTCATG GTACCTGGGC AAGTGTCCTG GAACTACAAC 
TTCATGGGTG TCCGTCATGA CCCCAACATG AAGTACGACC TGCAGCTGTC CAACCCTAAA 
GAGTTCTATC ATGAGGTTCA CAGGCCCTCT CACTTCCTCA ACTTTGCCTC TCTGCAAGAG 
GGTGAAATCT ACAACGCCGA CCGTGAGGAT ATGTATGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prpf8 ( XM_007260888.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007260950.1 Astyanax mexicanus pre-mRNA processing factor 8 (prpf8), mRNA.

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CloneID	OAs42413
Clone ID Related Accession (Same CDS sequence)	XM_007260888.2
Accession Version	XM_007260888.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	pre-mRNA-processing-splicing factor 8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749496.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007260888.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTA CATTCCCCTA CAGAGGTGTC CCTGGTGTTC CTCCTCCTGC CCCGGTGCCA 
GACTACATGT CTGAGGAGAA GCTTCAAGAA AAAGCCCGGA AATGGCAGCA GCTGCAGGCA 
AAACGCTACT CGGAGAAGAG GAAATTTGGT TTTGTCGATG CTCAGAAAGA GGACATGCCA 
CCAGAGCATG TGCGGAAAAT CATTCGGGAC CATGGAGATA TGACCAACAG GAAATTTCGT 
CACGACAAAC GAGTCTATCT TGGGGCTCTG AAGTACATGC CTCATGCAGT ACTGAAACTG 
CTGGAGAATA TGCCCATGCC TTGGGAGCAG ATCAGAGATG TGCCAGTGCT GTACCACATC 
ACAGGAGCCA TTTCCTTTGT AAATGAAATT CCTTGGGTCA TTGAACCAGT TTACATTGCA 
CAATGGGGTA CCATGTGGAT CATGATGCGT CGTGAGAAAA GAGACAGAAG GCACTTCAAG 
CGTATGCGCT TTCCTCCCTT TGATGATGAG GAGCCCCCAC TGGACTATGC TGATAACATT 
CTGGATGTGG AGCCCCTGGA AGCCATTCAG ATGGAAATGG ACCCTGAGGA AGATTGCTCA 
GTTGTAGAAT GGTTTTACGA ACATCAGCCA CTGAAGGATG CTACCAAATA TGTCAATGGG 
ACGACATACA GACGTTGGCA GTTCACCTTG CCAATGATGT CCACTCTTTA TCGGTTAGCC 
AACCAGCTGC TAACTGATTT AGTGGATAGG AACTACTTCT ATTTGTTTGA CCTGAAGGCC 
TTCTTCACTT CAAAGGCCTT AAACATGGCC ATTCCTGGAG GGCCCAAATT TGAACCCCTG 
GTCAGAGACA TCAATCTACA GGATGAAGAC TGGAATGAGT TTAACGACAT CAACAAGATC 
ATCATCAGAC AGCCGATTAG AACAGAGTAC AAGATTGCTT TCCCATACTT GTACAATAAT 
CTTCCACATC ACGTCCATCT CACTTGGTAC CACACTCCTA ATGTGGTGTT CATCAAGACA 
GAGGATCCTG ATTTGCCTGC TTTCTACTTT GATCCTCTCA TCAATCCCAT CTCTCACAGA 
CATTCTGTAA AAAGCCAAGA GCCACTGCCA GACGATGACG AGGAATTTGA GCTTCCTGAG 
TATGTGGAGC CTTTCCTCAA AGAGACGCCT CTTTATACTG ACAACACAGC CAATGGGATT 
GCCCTTCTCT GGGCCCCACG TCCGTTCAAC CTGCGCTCCG GAAGGACCAG GCGTGCCATC 
GATGTTCCCC TGATCAAGAA TTGGTACAGG GAACACTGCC CTGCTGGACA GCCTGTGAAA 
GTACGTGTGT CCTACCAGAA GCTCCTGAAG TACTATGTTC TCAATGCGCT GAAACACAGA 
CCACCTAAAG CGCAGAAGAA GAGATACCTT TTCCGATCCT TCAAGGCCAC AAAATTCTTC 
CAGTCCACAA AGCTAGACTG GGTGGAGGTG GGCCTGCAGG TTTGCCGACA AGGTTACAAC 
ATGCTGAACC TGCTGATTCA CAGAAAGAAC CTCAACTACT TGCACTTGGA CTACAATTTC 
AACTTGAAGC CTGTCAAAAC CCTCACCACA AAAGAGCGTA AAAAGTCCCG ATTCGGGAAT 
GCGTTCCACT TGTGCCGTGA GGTTCTCCGT CTGAGCAAGT TGGTAGTGGA CAGTCATGTA 
CAGTACAGAC TGGGAAATGT TGATGCATTC CAGTTGGCAG ATGGGCTGCA GTACATCTTT 
GCCCATGTGG GTCAGCTGAC TGGCATGTAC CGTTACAAGT ACAAGTTGAT GAGGCAGATC 
AGGATGTGCA AAGACTTGAA GCATCTCATC TACTACCGCT TCAATACAGG TCCAGTTGGG 
AAGGGTCCAG GTTGTGGTTT CTGGGCTCCA GGATGGAGAG TTTGGCTCTT CTTTATGAGA 
GGCATTACTC CTCTTCTGGA GAGATGGCTT GGCAACCTGC TGGCCAGGCA GTTCGAAGGT 
CGTCATTCCA AGGGAGTTGC AAAAACTGTC ACCAAGCAGC GTGTGGAGTC TCACTTTGAT 
CTTGAGCTGA GAGCTGCTGT GATGCATGAC ATCCTGGACA TGATGCCTGA AGGCATCAAA 
CAGAACAAGG CAAGGACCAT CCTGCAGCAC CTCAGTGAGT CCTGGAGATG CTGGAAGGCT 
AACATTCCTT GGAAGGTCCC AGGACTTCCA ACCCCCATCG AGAACATGAT CTTGCGCTAT 
GTGAAGGCCA AAGCTGATTG GTGGACCAAC ACAGCCCATT ACAACAGAGA GCGAATTAGG 
AGAGGTGCTA CTGTGGACAA GACTGTCTGC AAGAAAAACC TTGGCCGCCT CACACGTCTG 
TACCTGAAGG CTGAGCAGGA GAGGCAGCAC AACTACTTGA AGGATGGTCC ATACATCACT 
GCAGAAGAGG CGGTGGCCAT CTACACCACT ACTGTCCATT GGCTGGAGAG TCGGCGCTTC 
TCTCCAATTC CATTCCCTCC TCTCTCCTAC AAACACGACA CCAAGCTTCT CATCCTGGCT 
CTGGAGAGGC TCAAAGAAGC CTACAGTGTG AAGTCAAGGT TGAATCAGTC TCAGCGTGAG 
GAGTTGGGTC TGATCGAGCA GGCGTATGAC AACCCTCACG AGGCTCTGTC CAGGATCAAG 
CGTCACCTCC TCACCCAGAG AGCTTTCAAA GAGGTGGGCA TTGAGTTCAT GGACCTTTAC 
AGCCACTTGG TACCGGTGTA TGATGTGGAG CCCCTGGAGA AGATCACTGA TGCATACCTG 
GATCAGTACT TGTGGTACGA GGCTGACAAG CGCAGGCTTT TCCCACCGTG GATCAAACCT 
GCCGACACAG AGCCACCTCC ACTTTTGGTA TACAAATGGT GCCAAGGAAT CAACAATCTG 
CAGGATGTGT GGGAAACAAC CGAGGGAGAA TGCAACGTCA TGCTGGAGTC ACGCTATGAG 
AAAATGTATG AAAAGATCGA CTTGACGCTG CTCAACAGGC TGCTGCGTCT CATTGTTGAC 
CACAACATTG CTGATTATAT GACTGCCAAG AACAACGTTG TCATCAACTA CAAGGACATG 
AACCACACCA ACTCGTACGG CATCATTCGT GGCCTGCAGT TTGCTTCCTT CATTGTCCAG 
TACTATGGGC TTGTGATGGA CCTTCTTGTT CTGGGTCTTC ATCGGGCCAG TGAAATGGCA 
GGACCTCCCC AGATGCCCAA TGACTTCCTC TGCTTTCAGG ACAGTGCTAC AGAGGGTGCA 
CATCCTATTC GCCTTTACTG CAGATACATC GATCGCATCC ACATGTTCTT CAGGTTTTCT 
GCTGATGAGG CCAGAGATCT GATTCAGCGG TACCTAACTG AACACCCAGA CCCCAACAAT 
GAAAACATTG TGGGCTACAA CAATAAGAAA TGCTGGCCTA GAGATGCCAG GATGAGGTTG 
ATGAAGCATG ATGTTAACCT GGGTCGCGCT GTGTTCTGGG ACATTAAGAA TCGCCTTCCT 
CGCTCTGTGA CCACGGTGCA GTGGGAGAAC AGCTTTGTCT CAGTCTACAG TAAAGACAAT 
CCCAACCTGC TCTTTAACAT GTGTGGCTTT GAGTGTCGTA TCCTGCCAAA GTGCCGCACC 
AGTTATGAGG AGTTCACCCA CAAAGATGGT GTGTGGAACC TGCAGAATGA GGTGACCAAG 
GAAAGGACAG CTCAGTGCTT CCTGCGAGTG GATGATGAAT CTATGCAGAG GTTTCACAAC 
CGAGTGCGAC AGATTCTCAT GGCTTCTGGA TCTACCACTT TCACCAAGAT CGTGAACAAA 
TGGAACACAG CTTTGATTGG TCTGATGACC TACTTCCGTG AGGCTGTGGT CAACACTCAG 
GAGTTGCTGG ACCTTCTAGT GAAGTGCGAG AATAAGATCC AGACCCGTAT TAAGATCGGT 
CTGAACTCCA AAATGCCTAG CCGTTTCCCT CCTGTGGTCT TCTACACCCC CAAGGAGCTG 
GGTGGACTGG GCATGTTGTC CATGGGCCAT GTGCTCATCC CACAGTCTGA CCTGAGGTGG 
TCCAAACAAA CGGATGTAGG AATCACTCAT TTCCGCTCTG GTATGAGTCA CGAAGAGGAT 
CAGCTGATTC CCAATCTCTA TCGCTACATT CAGCCTTGGG AGAGTGAGTT CATTGACTCT 
CAGAGAGTCT GGGCTGAATA TGCCCTGAAG AGACAGGAGG CCATTGCCCA GAACAGGCGC 
TTGACTCTTG AGGATTTGGA GGATTCATGG GACAGAGGTA TTCCTCGTAT CAACACCCTC 
TTCCAAAAGG ACAGGCACAC ACTGGCCTAT GACAAGGGCT GGAGAGTCAG AACAGATTTC 
AAACAGTACC AGGTGCTGAA GCAAAACCCA TTCTGGTGGA CACATCAGAG GCACGACGGG 
AAACTGTGGA ATCTGAACAA TTACCGCACA GATATGATCC AGGCTTTGGG AGGAGTGGAG 
GGAATTTTGG AGCACACCCT CTTCAAGGGC ACATACTTTC CAACATGGGA AGGTCTCTTC 
TGGGAAAAGG CCAGTGGCTT TGAGGAGTCC ATGAAATGGA AGAAACTGAC CAACGCTCAG 
AGATCTGGTC TGAACCAGAT CCCCAATCGA CGATTCACTC TGTGGTGGTC TCCCACTATC 
AACAGAGCTA ATGTCTATGT GGGCTTCCAG GTGCAGTTGG ATCTCACTGG AATCTTCATG 
CATGGCAAAA TTCCAACACT CAAGATCTCC TTGATTCAGA TCTTCAGGGC TCACTTGTGG 
CAAAAGATTC ATGAGAGCAT TGTCATGGAT CTTTGTCAGG TGTTTGACCA AGAGTTGGAT 
GCTCTGGAGA TTGAGACTGT CCAGAAGGAA ACCATCCATC CCAGGAAGTC GTACAAAATG 
AACTCCTCCT GTGCAGACAT TCTGCTGTTT GCCTCCTACA AGTGGAATGT CTCCCGACCA 
TCACTGCTTG CTGACTCAAA GGATGTGATG GACAGTACCA CCACTCAGAA GTACTGGATT 
GACATTCAGC TCAGATGGGG AGATTATGAC TCCCATGACA TTGAAAGATA TGCTAGAGCC 
AAGTTCCTGG ACTACACCAC AGATAACATG AGTATCTACC CATCGCCCAC TGGTGTGCTG 
ATTGCCATTG ATTTGGCCTA CAATCTGCAC AGTGCCTATG GAAACTGGTT CCCTGGCAGC 
AAGCCTCTGA TCCAACAGGC CATGGCTAAG ATCATGAAGG CCAATCCTGC CCTTTATGTG 
CTAAGGGAGC GCATCCGCAA GGGTCTGCAG CTTTACTCCT CAGAGCCCAC TGAGCCCTAC 
CTGTCCTCCC AGAATTATGG AGAGCTCTTC TCTAACCAGA TCATCTGGTT TGTGGATGAC 
ACTAATGTGT ACCGTGTCAC CATCCACAAG ACCTTTGAGG GCAACTTGAC AACGAAACCA 
ATCAATGGAG CCATTTTCAT CTTCAATCCA AGAACTGGAC AGCTCTTCCT GAAGATCATT 
CACACTTCTG TGTGGGCTGG ACAGAAGCGT CTGGGACAGC TGGCCAAGTG GAAGACTGCT 
GAGGAAGTTG CTGCTCTTAT TCGATCACTG CCTGTTGAAG AGCAGCCCAA GCAGATCATT 
GTCACCAGAA AGGGCATGTT GGATCCTCTT GAGGTGCACT TGTTGGATTT CCCTAACATT 
GTGATCAAGG GATCCGAGCT ACAGCTGCCC TTCCAGGCCT GTCTGAAGGT GGAGAAGTTT 
GGAGACCTTA TTCTGAAGGC CACTGAGCCT CAGATGGTTC TGTTCAATCT GTATGATGAC 
TGGCTCAAGA CGATCTCGTC TTACACAGCT TTCTCACGTC TGATCTTGAT CCTGAGAGCT 
CTGCATGTGA ACAATGACCG TGCAAAGGTG ATCCTGAAGC CAGACAAAAC CACCATCACC 
GAGCCACACC ATATCTGGCC CACTTTAACT GACGAGGAGT GGATCAAGGT GGAGGTGCAG 
CTCAAAGATC TCATTCTGGC TGACTATGGC AAGAAGAACA ACGTGAACGT GGCTTCTTTG 
ACTCAGTCTG AGATCAGGGA CATCATCCTG GGTATGGAGA TTTCAGCTCC TTCCCAGCAG 
AGACAGCAGA TTGCTGAGAT AGAGAAGCAG ACCAAAGAGC AGTCTCAGTT AACAGCCACC 
CAGACCCGCA CCGTGAACAA GCACGGTGAT GAGATCATCA CTTCCACTAC CAGCAATTAC 
GAGACACAAA CATTTTCCTC CAAGACTGAA TGGAGGGTCA GGGCCATCTC TGCAGCCAAC 
TTGCACTTGC GAACCAATCA CATCTACGTG TCTTCAGATG ACATTAAGGA AACTGGATAC 
ACTTACATTC TGCCCAAGAA TGTGCTGAAG AAGTTCATCT GCATCTCCGA CTTGCGTGCT 
CAAATTGCAG GCTATCTGTA TGGCACTAGT CCTCCAGATA ATCCCCAAGT GAAGGAGATT 
CGCTGCATTG TGATGGTGCC TCAGTGGGGA ACACACCAGA CTGTTCATCT GCCCAATCAG 
CTGCCACAGC ATGAATATCT AAAGGAAATG GAGCCACTTG GCTGGATCCA CACCCAGCCC 
AATGAATCTC CTCAGCTTTC TCCTCAGGAT GTGACCACAC ATGCCAAGGT CATGGCAGAC 
AACCCATCCT GGGATGGGGA GAAAACCATC ATTATTACCT GCAGTTTCAC CCCAGGATCC 
TGTACCCTTA CTGCTTATAA ACTGACTCCT AGTGGATATG AATGGGGCAG GCAGAACACA 
GACAAGGGCA ACAACCCTAA GGGTTACCTG CCATCCCATT ACGAGAGGGT GCAGATGCTG 
CTGTCTGATC GCTTCCTTGG TTTCTTCATG GTACCTGGGC AAGTGTCCTG GAACTACAAC 
TTCATGGGTG TCCGTCATGA CCCCAACATG AAGTACGACC TGCAGCTGTC CAACCCTAAA 
GAGTTCTATC ATGAGGTTCA CAGGCCCTCT CACTTCCTCA ACTTTGCCTC TCTGCAAGAG 
GGTGAAATCT ACAACGCCGA CCGTGAGGAT ATGTATGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007260950.1
CDS	125..7126
Translation	MAATFPYRGVPGVPPPAPVPDYMSEEKLQEKARKWQQLQAKRYSEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYIAQWGTMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQMEMDPEEDCSVVEWFYEHQPLKDATKYVNGTTYRRWQFTLPMMSTLYRLANQLLTDLVDRNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEYVEPFLKETPLYTDNTANGIALLWAPRPFNLRSGRTRRAIDVPLIKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLSKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAPGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSESWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAIYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETTEGECNVMLESRYEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLCFQDSATEGAHPIRLYCRYIDRIHMFFRFSADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGTSPPDNPQVKEIRCIVMVPQWGTHQTVHLPNQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKVMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPGQVSWNYNFMGVRHDPNMKYDLQLSNPKEFYHEVHRPSHFLNFASLQEGEIYNADREDMYA

Target ORF information:

RefSeq Version	XM_007260888.2
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus pre-mRNA processing factor 8 (prpf8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007260888.2

ORF Insert Sequence:

ATGGCAGCTA CATTCCCCTA CAGAGGTGTC CCTGGTGTTC CTCCTCCTGC CCCGGTGCCA 
GACTACATGT CTGAGGAGAA GCTTCAAGAA AAAGCCCGGA AATGGCAGCA GCTGCAGGCA 
AAACGCTACT CGGAGAAGAG GAAATTTGGT TTTGTCGATG CTCAGAAAGA GGACATGCCA 
CCAGAGCATG TGCGGAAAAT CATTCGGGAC CATGGAGATA TGACCAACAG GAAATTTCGT 
CACGACAAAC GAGTCTATCT TGGGGCTCTG AAGTACATGC CTCATGCAGT ACTGAAACTG 
CTGGAGAATA TGCCCATGCC TTGGGAGCAG ATCAGAGATG TGCCAGTGCT GTACCACATC 
ACAGGAGCCA TTTCCTTTGT AAATGAAATT CCTTGGGTCA TTGAACCAGT TTACATTGCA 
CAATGGGGTA CCATGTGGAT CATGATGCGT CGTGAGAAAA GAGACAGAAG GCACTTCAAG 
CGTATGCGCT TTCCTCCCTT TGATGATGAG GAGCCCCCAC TGGACTATGC TGATAACATT 
CTGGATGTGG AGCCCCTGGA AGCCATTCAG ATGGAAATGG ACCCTGAGGA AGATTGCTCA 
GTTGTAGAAT GGTTTTACGA ACATCAGCCA CTGAAGGATG CTACCAAATA TGTCAATGGG 
ACGACATACA GACGTTGGCA GTTCACCTTG CCAATGATGT CCACTCTTTA TCGGTTAGCC 
AACCAGCTGC TAACTGATTT AGTGGATAGG AACTACTTCT ATTTGTTTGA CCTGAAGGCC 
TTCTTCACTT CAAAGGCCTT AAACATGGCC ATTCCTGGAG GGCCCAAATT TGAACCCCTG 
GTCAGAGACA TCAATCTACA GGATGAAGAC TGGAATGAGT TTAACGACAT CAACAAGATC 
ATCATCAGAC AGCCGATTAG AACAGAGTAC AAGATTGCTT TCCCATACTT GTACAATAAT 
CTTCCACATC ACGTCCATCT CACTTGGTAC CACACTCCTA ATGTGGTGTT CATCAAGACA 
GAGGATCCTG ATTTGCCTGC TTTCTACTTT GATCCTCTCA TCAATCCCAT CTCTCACAGA 
CATTCTGTAA AAAGCCAAGA GCCACTGCCA GACGATGACG AGGAATTTGA GCTTCCTGAG 
TATGTGGAGC CTTTCCTCAA AGAGACGCCT CTTTATACTG ACAACACAGC CAATGGGATT 
GCCCTTCTCT GGGCCCCACG TCCGTTCAAC CTGCGCTCCG GAAGGACCAG GCGTGCCATC 
GATGTTCCCC TGATCAAGAA TTGGTACAGG GAACACTGCC CTGCTGGACA GCCTGTGAAA 
GTACGTGTGT CCTACCAGAA GCTCCTGAAG TACTATGTTC TCAATGCGCT GAAACACAGA 
CCACCTAAAG CGCAGAAGAA GAGATACCTT TTCCGATCCT TCAAGGCCAC AAAATTCTTC 
CAGTCCACAA AGCTAGACTG GGTGGAGGTG GGCCTGCAGG TTTGCCGACA AGGTTACAAC 
ATGCTGAACC TGCTGATTCA CAGAAAGAAC CTCAACTACT TGCACTTGGA CTACAATTTC 
AACTTGAAGC CTGTCAAAAC CCTCACCACA AAAGAGCGTA AAAAGTCCCG ATTCGGGAAT 
GCGTTCCACT TGTGCCGTGA GGTTCTCCGT CTGAGCAAGT TGGTAGTGGA CAGTCATGTA 
CAGTACAGAC TGGGAAATGT TGATGCATTC CAGTTGGCAG ATGGGCTGCA GTACATCTTT 
GCCCATGTGG GTCAGCTGAC TGGCATGTAC CGTTACAAGT ACAAGTTGAT GAGGCAGATC 
AGGATGTGCA AAGACTTGAA GCATCTCATC TACTACCGCT TCAATACAGG TCCAGTTGGG 
AAGGGTCCAG GTTGTGGTTT CTGGGCTCCA GGATGGAGAG TTTGGCTCTT CTTTATGAGA 
GGCATTACTC CTCTTCTGGA GAGATGGCTT GGCAACCTGC TGGCCAGGCA GTTCGAAGGT 
CGTCATTCCA AGGGAGTTGC AAAAACTGTC ACCAAGCAGC GTGTGGAGTC TCACTTTGAT 
CTTGAGCTGA GAGCTGCTGT GATGCATGAC ATCCTGGACA TGATGCCTGA AGGCATCAAA 
CAGAACAAGG CAAGGACCAT CCTGCAGCAC CTCAGTGAGT CCTGGAGATG CTGGAAGGCT 
AACATTCCTT GGAAGGTCCC AGGACTTCCA ACCCCCATCG AGAACATGAT CTTGCGCTAT 
GTGAAGGCCA AAGCTGATTG GTGGACCAAC ACAGCCCATT ACAACAGAGA GCGAATTAGG 
AGAGGTGCTA CTGTGGACAA GACTGTCTGC AAGAAAAACC TTGGCCGCCT CACACGTCTG 
TACCTGAAGG CTGAGCAGGA GAGGCAGCAC AACTACTTGA AGGATGGTCC ATACATCACT 
GCAGAAGAGG CGGTGGCCAT CTACACCACT ACTGTCCATT GGCTGGAGAG TCGGCGCTTC 
TCTCCAATTC CATTCCCTCC TCTCTCCTAC AAACACGACA CCAAGCTTCT CATCCTGGCT 
CTGGAGAGGC TCAAAGAAGC CTACAGTGTG AAGTCAAGGT TGAATCAGTC TCAGCGTGAG 
GAGTTGGGTC TGATCGAGCA GGCGTATGAC AACCCTCACG AGGCTCTGTC CAGGATCAAG 
CGTCACCTCC TCACCCAGAG AGCTTTCAAA GAGGTGGGCA TTGAGTTCAT GGACCTTTAC 
AGCCACTTGG TACCGGTGTA TGATGTGGAG CCCCTGGAGA AGATCACTGA TGCATACCTG 
GATCAGTACT TGTGGTACGA GGCTGACAAG CGCAGGCTTT TCCCACCGTG GATCAAACCT 
GCCGACACAG AGCCACCTCC ACTTTTGGTA TACAAATGGT GCCAAGGAAT CAACAATCTG 
CAGGATGTGT GGGAAACAAC CGAGGGAGAA TGCAACGTCA TGCTGGAGTC ACGCTATGAG 
AAAATGTATG AAAAGATCGA CTTGACGCTG CTCAACAGGC TGCTGCGTCT CATTGTTGAC 
CACAACATTG CTGATTATAT GACTGCCAAG AACAACGTTG TCATCAACTA CAAGGACATG 
AACCACACCA ACTCGTACGG CATCATTCGT GGCCTGCAGT TTGCTTCCTT CATTGTCCAG 
TACTATGGGC TTGTGATGGA CCTTCTTGTT CTGGGTCTTC ATCGGGCCAG TGAAATGGCA 
GGACCTCCCC AGATGCCCAA TGACTTCCTC TGCTTTCAGG ACAGTGCTAC AGAGGGTGCA 
CATCCTATTC GCCTTTACTG CAGATACATC GATCGCATCC ACATGTTCTT CAGGTTTTCT 
GCTGATGAGG CCAGAGATCT GATTCAGCGG TACCTAACTG AACACCCAGA CCCCAACAAT 
GAAAACATTG TGGGCTACAA CAATAAGAAA TGCTGGCCTA GAGATGCCAG GATGAGGTTG 
ATGAAGCATG ATGTTAACCT GGGTCGCGCT GTGTTCTGGG ACATTAAGAA TCGCCTTCCT 
CGCTCTGTGA CCACGGTGCA GTGGGAGAAC AGCTTTGTCT CAGTCTACAG TAAAGACAAT 
CCCAACCTGC TCTTTAACAT GTGTGGCTTT GAGTGTCGTA TCCTGCCAAA GTGCCGCACC 
AGTTATGAGG AGTTCACCCA CAAAGATGGT GTGTGGAACC TGCAGAATGA GGTGACCAAG 
GAAAGGACAG CTCAGTGCTT CCTGCGAGTG GATGATGAAT CTATGCAGAG GTTTCACAAC 
CGAGTGCGAC AGATTCTCAT GGCTTCTGGA TCTACCACTT TCACCAAGAT CGTGAACAAA 
TGGAACACAG CTTTGATTGG TCTGATGACC TACTTCCGTG AGGCTGTGGT CAACACTCAG 
GAGTTGCTGG ACCTTCTAGT GAAGTGCGAG AATAAGATCC AGACCCGTAT TAAGATCGGT 
CTGAACTCCA AAATGCCTAG CCGTTTCCCT CCTGTGGTCT TCTACACCCC CAAGGAGCTG 
GGTGGACTGG GCATGTTGTC CATGGGCCAT GTGCTCATCC CACAGTCTGA CCTGAGGTGG 
TCCAAACAAA CGGATGTAGG AATCACTCAT TTCCGCTCTG GTATGAGTCA CGAAGAGGAT 
CAGCTGATTC CCAATCTCTA TCGCTACATT CAGCCTTGGG AGAGTGAGTT CATTGACTCT 
CAGAGAGTCT GGGCTGAATA TGCCCTGAAG AGACAGGAGG CCATTGCCCA GAACAGGCGC 
TTGACTCTTG AGGATTTGGA GGATTCATGG GACAGAGGTA TTCCTCGTAT CAACACCCTC 
TTCCAAAAGG ACAGGCACAC ACTGGCCTAT GACAAGGGCT GGAGAGTCAG AACAGATTTC 
AAACAGTACC AGGTGCTGAA GCAAAACCCA TTCTGGTGGA CACATCAGAG GCACGACGGG 
AAACTGTGGA ATCTGAACAA TTACCGCACA GATATGATCC AGGCTTTGGG AGGAGTGGAG 
GGAATTTTGG AGCACACCCT CTTCAAGGGC ACATACTTTC CAACATGGGA AGGTCTCTTC 
TGGGAAAAGG CCAGTGGCTT TGAGGAGTCC ATGAAATGGA AGAAACTGAC CAACGCTCAG 
AGATCTGGTC TGAACCAGAT CCCCAATCGA CGATTCACTC TGTGGTGGTC TCCCACTATC 
AACAGAGCTA ATGTCTATGT GGGCTTCCAG GTGCAGTTGG ATCTCACTGG AATCTTCATG 
CATGGCAAAA TTCCAACACT CAAGATCTCC TTGATTCAGA TCTTCAGGGC TCACTTGTGG 
CAAAAGATTC ATGAGAGCAT TGTCATGGAT CTTTGTCAGG TGTTTGACCA AGAGTTGGAT 
GCTCTGGAGA TTGAGACTGT CCAGAAGGAA ACCATCCATC CCAGGAAGTC GTACAAAATG 
AACTCCTCCT GTGCAGACAT TCTGCTGTTT GCCTCCTACA AGTGGAATGT CTCCCGACCA 
TCACTGCTTG CTGACTCAAA GGATGTGATG GACAGTACCA CCACTCAGAA GTACTGGATT 
GACATTCAGC TCAGATGGGG AGATTATGAC TCCCATGACA TTGAAAGATA TGCTAGAGCC 
AAGTTCCTGG ACTACACCAC AGATAACATG AGTATCTACC CATCGCCCAC TGGTGTGCTG 
ATTGCCATTG ATTTGGCCTA CAATCTGCAC AGTGCCTATG GAAACTGGTT CCCTGGCAGC 
AAGCCTCTGA TCCAACAGGC CATGGCTAAG ATCATGAAGG CCAATCCTGC CCTTTATGTG 
CTAAGGGAGC GCATCCGCAA GGGTCTGCAG CTTTACTCCT CAGAGCCCAC TGAGCCCTAC 
CTGTCCTCCC AGAATTATGG AGAGCTCTTC TCTAACCAGA TCATCTGGTT TGTGGATGAC 
ACTAATGTGT ACCGTGTCAC CATCCACAAG ACCTTTGAGG GCAACTTGAC AACGAAACCA 
ATCAATGGAG CCATTTTCAT CTTCAATCCA AGAACTGGAC AGCTCTTCCT GAAGATCATT 
CACACTTCTG TGTGGGCTGG ACAGAAGCGT CTGGGACAGC TGGCCAAGTG GAAGACTGCT 
GAGGAAGTTG CTGCTCTTAT TCGATCACTG CCTGTTGAAG AGCAGCCCAA GCAGATCATT 
GTCACCAGAA AGGGCATGTT GGATCCTCTT GAGGTGCACT TGTTGGATTT CCCTAACATT 
GTGATCAAGG GATCCGAGCT ACAGCTGCCC TTCCAGGCCT GTCTGAAGGT GGAGAAGTTT 
GGAGACCTTA TTCTGAAGGC CACTGAGCCT CAGATGGTTC TGTTCAATCT GTATGATGAC 
TGGCTCAAGA CGATCTCGTC TTACACAGCT TTCTCACGTC TGATCTTGAT CCTGAGAGCT 
CTGCATGTGA ACAATGACCG TGCAAAGGTG ATCCTGAAGC CAGACAAAAC CACCATCACC 
GAGCCACACC ATATCTGGCC CACTTTAACT GACGAGGAGT GGATCAAGGT GGAGGTGCAG 
CTCAAAGATC TCATTCTGGC TGACTATGGC AAGAAGAACA ACGTGAACGT GGCTTCTTTG 
ACTCAGTCTG AGATCAGGGA CATCATCCTG GGTATGGAGA TTTCAGCTCC TTCCCAGCAG 
AGACAGCAGA TTGCTGAGAT AGAGAAGCAG ACCAAAGAGC AGTCTCAGTT AACAGCCACC 
CAGACCCGCA CCGTGAACAA GCACGGTGAT GAGATCATCA CTTCCACTAC CAGCAATTAC 
GAGACACAAA CATTTTCCTC CAAGACTGAA TGGAGGGTCA GGGCCATCTC TGCAGCCAAC 
TTGCACTTGC GAACCAATCA CATCTACGTG TCTTCAGATG ACATTAAGGA AACTGGATAC 
ACTTACATTC TGCCCAAGAA TGTGCTGAAG AAGTTCATCT GCATCTCCGA CTTGCGTGCT 
CAAATTGCAG GCTATCTGTA TGGCACTAGT CCTCCAGATA ATCCCCAAGT GAAGGAGATT 
CGCTGCATTG TGATGGTGCC TCAGTGGGGA ACACACCAGA CTGTTCATCT GCCCAATCAG 
CTGCCACAGC ATGAATATCT AAAGGAAATG GAGCCACTTG GCTGGATCCA CACCCAGCCC 
AATGAATCTC CTCAGCTTTC TCCTCAGGAT GTGACCACAC ATGCCAAGGT CATGGCAGAC 
AACCCATCCT GGGATGGGGA GAAAACCATC ATTATTACCT GCAGTTTCAC CCCAGGATCC 
TGTACCCTTA CTGCTTATAA ACTGACTCCT AGTGGATATG AATGGGGCAG GCAGAACACA 
GACAAGGGCA ACAACCCTAA GGGTTACCTG CCATCCCATT ACGAGAGGGT GCAGATGCTG 
CTGTCTGATC GCTTCCTTGG TTTCTTCATG GTACCTGGGC AAGTGTCCTG GAACTACAAC 
TTCATGGGTG TCCGTCATGA CCCCAACATG AAGTACGACC TGCAGCTGTC CAACCCTAAA 
GAGTTCTATC ATGAGGTTCA CAGGCCCTCT CACTTCCTCA ACTTTGCCTC TCTGCAAGAG 
GGTGAAATCT ACAACGCCGA CCGTGAGGAT ATGTATGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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prpf8 cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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