ORF » Species Summary » Astyanax mexicanus » otof cDNA ORF clone

otof cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

The following otof gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the otof cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAs123562 XM_022686231.1
Latest version!		 Astyanax mexicanus otoferlin (otof), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OAs42394 XM_007260871.2
Latest version!		 Astyanax mexicanus otoferlin (otof), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OAs42397 XM_007260874.2
Latest version!		 Astyanax mexicanus otoferlin (otof), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OAs123558 XM_015608738.2
Latest version!		 Astyanax mexicanus otoferlin (otof), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OAs123559 XM_022686215.1
Latest version!		 Astyanax mexicanus otoferlin (otof), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OAs123560 XM_022686222.1
Latest version!		 Astyanax mexicanus otoferlin (otof), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OAs123561 XM_022686227.1
Latest version!		 Astyanax mexicanus otoferlin (otof), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OAs123563 XM_022686236.1
Latest version!		 Astyanax mexicanus otoferlin (otof), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OAs123564 XM_022686241.1
Latest version!		 Astyanax mexicanus otoferlin (otof), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OAs42390 XM_015608738.1
Latest version!		 Astyanax mexicanus otoferlin (otof), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OAs42391 XM_007260866.2
Latest version!		 Astyanax mexicanus otoferlin (otof), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OAs42392 XM_007260865.2
Latest version!		 Astyanax mexicanus otoferlin (otof), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OAs42393 XM_007260867.2
Latest version!		 Astyanax mexicanus otoferlin (otof), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OAs42395 XM_007260873.2
Latest version!		 Astyanax mexicanus otoferlin (otof), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OAs42396 XM_007260872.2
Latest version!		 Astyanax mexicanus otoferlin (otof), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OAs42398 XM_015608739.1
Latest version!		 Astyanax mexicanus otoferlin (otof), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    otof ( XM_022686231.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_022541952.1 Astyanax mexicanus otoferlin (otof), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OAs123562
    Clone ID Related Accession (Same CDS sequence) XM_022686231.1
    Accession Version XM_022686231.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1506873600000
    Organism Astyanax mexicanus(Mexican tetra)
    Product otoferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035899.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTTAG TGGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGATGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTGTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCTGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCTTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA ACTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATTGGTCC AGCTGGACGG ACAGAGCTTC CTCCCATTGA TGGGCCAACT 
    GATGTTGACC GAGGTCCCAT CTTACCTGTG CCTCTGGGCA TACGGCCCGT CCTCAGCAGA 
    TACCGCATAG AGGTCTTGTT CTGGGGGCTG CGGGACCTGA AGAGAATTAA CCTGGCGCAG 
    GTCGACAGGC CTCGAGTGGA CATTGAGTGT GCAGGCAAAG GAATCCAATC AGCTCTCAAT 
    CAGAATTACA AGAAAAACCC AAATTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGATCTG 
    CCAGAGAACG AGTTGCTTCA TCCTCCTCTG AATATTCGGG TGGTAGACTG CAGAGCATTT 
    GGCCGCTACA CATTGGTGGG CTCACATGCT GTAACCAGTC TGCGCAAGTT CATCTACATC 
    CCTCCTGACA AAAGGTCCAA TAACTGGGCT AATACAGGTG ACATCGTGGT CAACATGGAC 
    TCTGAGCCCA ATGTCAAGAA GATGGAGACT GTTGTAAAAC TGGATGCTAC CTCTGATGCT 
    GTTGTTAAAA TTGATGTGAA TGAGGAAGAA AAGGAAAAAG ACAAAAAGAA GAAAAAGAAA 
    AAGAAGGGAG AGGAGATAGA AGATGAGGAG CCAGATGAGA GCATGCTGGA CTGGTGGTCA 
    AAGTACTTTG CTTCAATAGA GACCATGATG GAGAACCTAC GAGTCCAGGA AGCAGCATGG 
    GCTGAGGCTG AGGAGAGAGA AGATTTGGAG ATTGCGGCAG AGGGAGCAGA AATCAAAAGT 
    GATGACTCTT GTACAAAAGG CACGAAGCCC AAAGAGAAGA ACAAGATAAA GAGCTCAAAG 
    GACAAGAAGA AGAGCCACGG CAGTGATGGG ACAGAGAAAA AAGTGCCCAA ACCCAAAGTA 
    GATGAGCTTT TGGTCTACAA TAAGGAGCTG GAGACTGAGT TTGATCACTT TGAAGACTGG 
    CTTCACACCT TCAACCTGTA CAGAGGGAAG GCCATAGATG ATGACGACCA AAACATTGTG 
    GATGACGACA GGATTGTGGG CCGATTTAAA GGGTCATTGT GCATGTACAA GCTGCCTCTG 
    TCAGATGAAA TCACCAGAGA TGTTGGATTC GATCCAAATA TGGGCATGTT TCAGAGTATC 
    CCACACAATG ATCCCATCAA TGTACTTGTG AGGGTTTATG TTATCAGGGC AACAGACCTT 
    CACCCTGCAG ATATAAACGG AAAAGCTGAT CCATATGTTG TTATCAAGCT GGGAAAGTCA 
    GAGATCAAGG ACAAGGAGAA CTACATCTCT AAACAGCTGA ATCCTGTCTT TGGGAAGTCC 
    TTTGACATTG AAGCCACGTT TCCAATGGAG TCTATGCTGA CAGTCTCAGT GTACGACTGG 
    GATTTAGTTG GCACTGATGA TTTGATCGGA GAGACCAAGA TTGACTTGGA GAACCGTTAC 
    TACAGCAAAC ACAGAGCCAC CTGTGGCATC GCATCCAATT ACTCTCTTCA TGGGTACAAT 
    GTGTGGCGAG ACCCTCAGAA GCCCACCCAG ATACTTGCAA AGTTGTGTAA GGAGGGCAAA 
    CTGGATGGAC CGCATTATGG CCCAGGTGGA AAGGTTAAAG TTGCAAACCG CATCTTTCTC 
    GGGCCAACGG ATATTGAAGA TGAAAATGGT CTAAAGAAGC AAACAGAAGA GCATTTGGCT 
    CTTACTGCAC TGAAACACTG GGAGGAAATT CCTCGAGTGG GCTGCAAACT CATCCCAGAA 
    CATGTAGAAA CCCGACCACT CCTCAACCCA GATAAACCTG GAATAGAGCA GGGTCGGCTT 
    GAGATGTGGG TGGATATGTT CCCAATGGAT GTTCCTTCAC CAGGACCAGC CATAGACATA 
    TCACCACGGA AACCAAAGAG ATATGAGCTC AGGGTGATAA TATGGAATAC TGACGAGGTA 
    ATTCTGGAAG ACGATGATTA CTTCACAGGG GAAAAGTCCA GTGACATTTT TGTCAGGGGC 
    TGGCTGAAAG GACAGCAGGA AGATAAGCAG GACACAGACG TTCACTACCA CTCTCTGACA 
    GGAGAGGGAA ACTTCAACTG GCGCTTTGTG TTTCCCTTCG ACTATCTCAT GGCTGAGGAG 
    AAAATTGTGA TCTCTAAGAA AGAGTCTTTG TTCTCCTGGG ACGAGACAGA GTACAAGATC 
    CCTGCCCGGC TCACCATGCA AGTGTGGGAC GCTGACCACT TCTCTGCTGA TGACTTCTTG 
    GGTGCGATTG AGCTGGACTT GAACCGGTTT CCTCGGGGAG CAAAAACAGC AAAGCAGTGC 
    TCCATCGACA TGGTGCTTAA TGAGCAAGAG CTGCCAACCA TCTCAATCTT CAAACAGAAA 
    AGGGTGAAGG GCTGGTGGCC TTTTGTGGCG CGAGATGAGA ATGATGACCT GGAGCTGACG 
    GGAAAAGTGG AGGCAGAGCT TCATCTAATG ACAGCGGAGG AAGCAGAGAA GAGTCCTGTG 
    GGCCTTGGAA GGAATGAACC AGACCCACTG GAGAAACCAA ACCGGCCCGA CACCACCTTC 
    CTCTGGTTCC TGAGTCCACT GAAGGCCATC CGCTACCTGG TGTGCAACCG CTACAAATGG 
    CTCATAATCA AGATTGTCCT GGCCCTGCTG CTGCTGATCA TGGTGGGCCT CTTCCTCTAC 
    AGCATGCCTG GCTACCTGGT CAAGAAGCTG CTGGGAGCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022541952.1
    CDS268..6249
    Translation

    Target ORF information:

    RefSeq Version XM_022686231.1
    Organism Astyanax mexicanus(Mexican tetra)
    Definition Astyanax mexicanus otoferlin (otof), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_022686231.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTTAG TGGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGATGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTGTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCTGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCTTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA ACTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATTGGTCC AGCTGGACGG ACAGAGCTTC CTCCCATTGA TGGGCCAACT 
    GATGTTGACC GAGGTCCCAT CTTACCTGTG CCTCTGGGCA TACGGCCCGT CCTCAGCAGA 
    TACCGCATAG AGGTCTTGTT CTGGGGGCTG CGGGACCTGA AGAGAATTAA CCTGGCGCAG 
    GTCGACAGGC CTCGAGTGGA CATTGAGTGT GCAGGCAAAG GAATCCAATC AGCTCTCAAT 
    CAGAATTACA AGAAAAACCC AAATTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGATCTG 
    CCAGAGAACG AGTTGCTTCA TCCTCCTCTG AATATTCGGG TGGTAGACTG CAGAGCATTT 
    GGCCGCTACA CATTGGTGGG CTCACATGCT GTAACCAGTC TGCGCAAGTT CATCTACATC 
    CCTCCTGACA AAAGGTCCAA TAACTGGGCT AATACAGGTG ACATCGTGGT CAACATGGAC 
    TCTGAGCCCA ATGTCAAGAA GATGGAGACT GTTGTAAAAC TGGATGCTAC CTCTGATGCT 
    GTTGTTAAAA TTGATGTGAA TGAGGAAGAA AAGGAAAAAG ACAAAAAGAA GAAAAAGAAA 
    AAGAAGGGAG AGGAGATAGA AGATGAGGAG CCAGATGAGA GCATGCTGGA CTGGTGGTCA 
    AAGTACTTTG CTTCAATAGA GACCATGATG GAGAACCTAC GAGTCCAGGA AGCAGCATGG 
    GCTGAGGCTG AGGAGAGAGA AGATTTGGAG ATTGCGGCAG AGGGAGCAGA AATCAAAAGT 
    GATGACTCTT GTACAAAAGG CACGAAGCCC AAAGAGAAGA ACAAGATAAA GAGCTCAAAG 
    GACAAGAAGA AGAGCCACGG CAGTGATGGG ACAGAGAAAA AAGTGCCCAA ACCCAAAGTA 
    GATGAGCTTT TGGTCTACAA TAAGGAGCTG GAGACTGAGT TTGATCACTT TGAAGACTGG 
    CTTCACACCT TCAACCTGTA CAGAGGGAAG GCCATAGATG ATGACGACCA AAACATTGTG 
    GATGACGACA GGATTGTGGG CCGATTTAAA GGGTCATTGT GCATGTACAA GCTGCCTCTG 
    TCAGATGAAA TCACCAGAGA TGTTGGATTC GATCCAAATA TGGGCATGTT TCAGAGTATC 
    CCACACAATG ATCCCATCAA TGTACTTGTG AGGGTTTATG TTATCAGGGC AACAGACCTT 
    CACCCTGCAG ATATAAACGG AAAAGCTGAT CCATATGTTG TTATCAAGCT GGGAAAGTCA 
    GAGATCAAGG ACAAGGAGAA CTACATCTCT AAACAGCTGA ATCCTGTCTT TGGGAAGTCC 
    TTTGACATTG AAGCCACGTT TCCAATGGAG TCTATGCTGA CAGTCTCAGT GTACGACTGG 
    GATTTAGTTG GCACTGATGA TTTGATCGGA GAGACCAAGA TTGACTTGGA GAACCGTTAC 
    TACAGCAAAC ACAGAGCCAC CTGTGGCATC GCATCCAATT ACTCTCTTCA TGGGTACAAT 
    GTGTGGCGAG ACCCTCAGAA GCCCACCCAG ATACTTGCAA AGTTGTGTAA GGAGGGCAAA 
    CTGGATGGAC CGCATTATGG CCCAGGTGGA AAGGTTAAAG TTGCAAACCG CATCTTTCTC 
    GGGCCAACGG ATATTGAAGA TGAAAATGGT CTAAAGAAGC AAACAGAAGA GCATTTGGCT 
    CTTACTGCAC TGAAACACTG GGAGGAAATT CCTCGAGTGG GCTGCAAACT CATCCCAGAA 
    CATGTAGAAA CCCGACCACT CCTCAACCCA GATAAACCTG GAATAGAGCA GGGTCGGCTT 
    GAGATGTGGG TGGATATGTT CCCAATGGAT GTTCCTTCAC CAGGACCAGC CATAGACATA 
    TCACCACGGA AACCAAAGAG ATATGAGCTC AGGGTGATAA TATGGAATAC TGACGAGGTA 
    ATTCTGGAAG ACGATGATTA CTTCACAGGG GAAAAGTCCA GTGACATTTT TGTCAGGGGC 
    TGGCTGAAAG GACAGCAGGA AGATAAGCAG GACACAGACG TTCACTACCA CTCTCTGACA 
    GGAGAGGGAA ACTTCAACTG GCGCTTTGTG TTTCCCTTCG ACTATCTCAT GGCTGAGGAG 
    AAAATTGTGA TCTCTAAGAA AGAGTCTTTG TTCTCCTGGG ACGAGACAGA GTACAAGATC 
    CCTGCCCGGC TCACCATGCA AGTGTGGGAC GCTGACCACT TCTCTGCTGA TGACTTCTTG 
    GGTGCGATTG AGCTGGACTT GAACCGGTTT CCTCGGGGAG CAAAAACAGC AAAGCAGTGC 
    TCCATCGACA TGGTGCTTAA TGAGCAAGAG CTGCCAACCA TCTCAATCTT CAAACAGAAA 
    AGGGTGAAGG GCTGGTGGCC TTTTGTGGCG CGAGATGAGA ATGATGACCT GGAGCTGACG 
    GGAAAAGTGG AGGCAGAGCT TCATCTAATG ACAGCGGAGG AAGCAGAGAA GAGTCCTGTG 
    GGCCTTGGAA GGAATGAACC AGACCCACTG GAGAAACCAA ACCGGCCCGA CACCACCTTC 
    CTCTGGTTCC TGAGTCCACT GAAGGCCATC CGCTACCTGG TGTGCAACCG CTACAAATGG 
    CTCATAATCA AGATTGTCCT GGCCCTGCTG CTGCTGATCA TGGTGGGCCT CTTCCTCTAC 
    AGCATGCCTG GCTACCTGGT CAAGAAGCTG CTGGGAGCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    otof ( XM_007260871.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007260933.1 Astyanax mexicanus otoferlin (otof), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OAs42394
    Clone ID Related Accession (Same CDS sequence) XM_007260871.2
    Accession Version XM_007260871.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1455033600000
    Organism Astyanax mexicanus(Mexican tetra)
    Product otoferlin isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749495.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007260871.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTTAG TTGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGACGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTCTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCGGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCCTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA GCTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATTGGTCC AGCTGGACGG ACAGAGCTTC CTCCCATTGA TGGGCCAACT 
    GATGTTGACC GAGGTCCCAT CTTACCTGTG CCTCTGGGCA TACGGCCCGT CCTCAGCAGA 
    TACCGCATAG AGGTCTTGTT CTGGGGGCTG CGGGACCTGA AGAGAATTAA CCTGGCGCAG 
    GTCGACAGGC CTCGAGTGGA CATTGAGTGT GCAGGCAAAG GAATCCAATC AGCTCTCATT 
    CAGAATTACA AGAAAAACCC AAATTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGATCTG 
    CCAGAGAACG AGTTGCTTCA TCCTCCTCTG AATATTCGGG TGGTAGACTG CAGAGCATTT 
    GGCCGCTACA CATTGGTGGG CTCACATGCT GTAACCAGTC TGCGCAAGTT CATCTACATC 
    CCTCCTGACA AAAGGTCCAA TAACTGGGCT AATACAGGTG ACATCGTGGT CAACATGGAC 
    TCTGAGCCCA ATGTCAAGAA GATGGAGACT GTTGTAAAAC TGGATGCTAC CTCTGATGCT 
    GTTGTTAAAA TTGATGTGAA TGAGGAAGAA AAGGAAAAAG ACAAAAAGAA GAAAAAGAAA 
    AAGAAGGGAG AGGAGATAGA AGATGAGGAG CCAGATGAGA GCATGCTGGA CTGGTGGTCA 
    AAGTACTTTG CTTCAATAGA GACCATGATG GAGAACCTAC GAGTCCAGGA AGCAGCATGG 
    GCTGAGGCTG AGGAGAGAGA AGATTTGGAG ATTGCGGCAG AGGGAGCAGA AATCAAAAGT 
    GATGACTCTT GTACAAAAGG CACGAAGCCC AAAGAGAAGA ACAAGATAAA GAGCTCAAAG 
    GACAAGAAGA AGAGCCACGG CAGTGATGGG ACAGAGAAAA AAGTGCCCAA ACCCAAAGTA 
    GACGAGCTTT TGGTCTACAA TAAGGAGCTG GAGACTGAGT TTGATCACTT TGAAGACTGG 
    CTTCACACCT TCAACCTGTA CAGAGGGAAG GCCATAGATG ATGATGACCA AAACATTGTG 
    GATGACGACA GGATTGTGGG CCGATTTAAA GGGTCATTGT GCATGTACAA GCTGCCTCTG 
    TCAGATGAAA TCACCAGAGA TGTTGGATTC GATCCAAATA TGGGCATGTT TCAGAGTATC 
    CCACACAATG ATCCCATCAA TGTACTTGTG AGGGTTTATG TTATCAGGGC AACAGACCTT 
    CACCCTGCAG ATATAAACGG AAAAGCTGAT CCATATGTTG TTATCAAGCT GGGAAAGTCA 
    GAGATCAAGG ACAAGGAGAA CTACATCTCT AAACAGCTGA ATCCTGTCTT TGGGAAGTCC 
    TTTGACATTG AAGCCACGTT TCCAATGGAG TCTATGCTGA CAGTCTCAGT GTACGACTGG 
    GATTTAGTTG GCACTGATGA TTTGATCGGA GAGACCAAGA TTGACTTGGA GAACCGTTAC 
    TACAGCAAAC ACAGAGCCAC CTGTGGCATC GCATCCAATT ACTCTCTTCA TGGGTACAAT 
    GTGTGGCGAG ACCCTCAGAA GCCCACCCAG ATACTTGCAA AGTTGTGTAA GGAGGGCAAA 
    CTGGATGGAC CGCATTATGG CCCAGGTGGA AAGGTTAAAG TTGCAAACCG CATCTTTCTC 
    GGGCCAACGG ATATTGAAGA TGAAAATGGT CTAAAGAAGC AAACAGAAGA GCATTTGGCT 
    CTTACTGCAC TGAAACACTG GGAGGAAATT CCTCGAGTGG GCTGCAAACT CATCCCAGAA 
    CATGTAGAAA CCCGACCACT CCTCAACCCA GATAAACCTG GAATAGAGCA GGGTCGGCTT 
    GAGATGTGGG TGGATATGTT CCCAATGGAT GTTCCTTCAC CAGGACCAGC CATAGACATA 
    TCACCACGGA AACCAAAGAG ATATGAGCTC AGGGTGATAA TATGGAATAC TGACGAGGTA 
    ATTCTGGAAG ACGATGATTA CTTCACAGGG GAAAAGTCCA GTGACATTTT TGTCAGGGGC 
    TGGCTGAAAG GACAGCAGGA AGATAAGCAG GACACAGACG TTCACTACCA CTCTCTGACA 
    GGAGAGGGAA ACTTCAACTG GCGCTTTGTG TTTCCCTTCG ACTATCTCAT GGCTGAGGAG 
    AAAATTGTGA TCTCTAAGAA AGAGTCTTTG TTCTCCTGGG ACGAGACAGA GTACAAGATC 
    CCTGCCCGGC TCACCATGCA AGTGTGGGAC GCTGACCACT TCTCTGCTGA TGACTTCTTG 
    GGTGCGATTG AGCTGGACTT GAACCGGTTT CCTCGGGGAG CAAAAACAGC AAAGCAGTGC 
    TCCATCGACA TGGTGCTTAA TGAGCAAGAG CTGCCAACCA TCTCAATCTT CAAACAGAAA 
    AGGGTGAAGG GCTGGTGGCC TTTTGTGGCG CGAGATGAGA ATGATGACCT GGAGCTGACG 
    GGAAAAGTGG AGGCAGAGCT TCATCTAATG ACAGCAGAGG AAGCAGAGAA GAGTCCTGTG 
    GGCCTTGGAA GGAATGAACC AGACCCACTG GAGAAACCAA ATCGCCCGGA CACCAGTCTC 
    ATGTGGTTCA TGAACCCCCT GAAGTCCCTG CGATACTTCA TTTGGCACAA CTACCGCTGG 
    CTGATCCTGA AGACCCTAGG GCTGATGCTG CTCCTCCTGC TCCTCGGCCT CTTTCTCTAC 
    TCCATCCCTG GATACCTGGT CAAGAAGCTG CTGGGGGCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_007260933.1
    CDS134..6115
    Translation

    Target ORF information:

    RefSeq Version XM_007260871.2
    Organism Astyanax mexicanus(Mexican tetra)
    Definition Astyanax mexicanus otoferlin (otof), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_007260871.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTTAG TTGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGACGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTCTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCGGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCCTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA GCTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATTGGTCC AGCTGGACGG ACAGAGCTTC CTCCCATTGA TGGGCCAACT 
    GATGTTGACC GAGGTCCCAT CTTACCTGTG CCTCTGGGCA TACGGCCCGT CCTCAGCAGA 
    TACCGCATAG AGGTCTTGTT CTGGGGGCTG CGGGACCTGA AGAGAATTAA CCTGGCGCAG 
    GTCGACAGGC CTCGAGTGGA CATTGAGTGT GCAGGCAAAG GAATCCAATC AGCTCTCATT 
    CAGAATTACA AGAAAAACCC AAATTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGATCTG 
    CCAGAGAACG AGTTGCTTCA TCCTCCTCTG AATATTCGGG TGGTAGACTG CAGAGCATTT 
    GGCCGCTACA CATTGGTGGG CTCACATGCT GTAACCAGTC TGCGCAAGTT CATCTACATC 
    CCTCCTGACA AAAGGTCCAA TAACTGGGCT AATACAGGTG ACATCGTGGT CAACATGGAC 
    TCTGAGCCCA ATGTCAAGAA GATGGAGACT GTTGTAAAAC TGGATGCTAC CTCTGATGCT 
    GTTGTTAAAA TTGATGTGAA TGAGGAAGAA AAGGAAAAAG ACAAAAAGAA GAAAAAGAAA 
    AAGAAGGGAG AGGAGATAGA AGATGAGGAG CCAGATGAGA GCATGCTGGA CTGGTGGTCA 
    AAGTACTTTG CTTCAATAGA GACCATGATG GAGAACCTAC GAGTCCAGGA AGCAGCATGG 
    GCTGAGGCTG AGGAGAGAGA AGATTTGGAG ATTGCGGCAG AGGGAGCAGA AATCAAAAGT 
    GATGACTCTT GTACAAAAGG CACGAAGCCC AAAGAGAAGA ACAAGATAAA GAGCTCAAAG 
    GACAAGAAGA AGAGCCACGG CAGTGATGGG ACAGAGAAAA AAGTGCCCAA ACCCAAAGTA 
    GACGAGCTTT TGGTCTACAA TAAGGAGCTG GAGACTGAGT TTGATCACTT TGAAGACTGG 
    CTTCACACCT TCAACCTGTA CAGAGGGAAG GCCATAGATG ATGATGACCA AAACATTGTG 
    GATGACGACA GGATTGTGGG CCGATTTAAA GGGTCATTGT GCATGTACAA GCTGCCTCTG 
    TCAGATGAAA TCACCAGAGA TGTTGGATTC GATCCAAATA TGGGCATGTT TCAGAGTATC 
    CCACACAATG ATCCCATCAA TGTACTTGTG AGGGTTTATG TTATCAGGGC AACAGACCTT 
    CACCCTGCAG ATATAAACGG AAAAGCTGAT CCATATGTTG TTATCAAGCT GGGAAAGTCA 
    GAGATCAAGG ACAAGGAGAA CTACATCTCT AAACAGCTGA ATCCTGTCTT TGGGAAGTCC 
    TTTGACATTG AAGCCACGTT TCCAATGGAG TCTATGCTGA CAGTCTCAGT GTACGACTGG 
    GATTTAGTTG GCACTGATGA TTTGATCGGA GAGACCAAGA TTGACTTGGA GAACCGTTAC 
    TACAGCAAAC ACAGAGCCAC CTGTGGCATC GCATCCAATT ACTCTCTTCA TGGGTACAAT 
    GTGTGGCGAG ACCCTCAGAA GCCCACCCAG ATACTTGCAA AGTTGTGTAA GGAGGGCAAA 
    CTGGATGGAC CGCATTATGG CCCAGGTGGA AAGGTTAAAG TTGCAAACCG CATCTTTCTC 
    GGGCCAACGG ATATTGAAGA TGAAAATGGT CTAAAGAAGC AAACAGAAGA GCATTTGGCT 
    CTTACTGCAC TGAAACACTG GGAGGAAATT CCTCGAGTGG GCTGCAAACT CATCCCAGAA 
    CATGTAGAAA CCCGACCACT CCTCAACCCA GATAAACCTG GAATAGAGCA GGGTCGGCTT 
    GAGATGTGGG TGGATATGTT CCCAATGGAT GTTCCTTCAC CAGGACCAGC CATAGACATA 
    TCACCACGGA AACCAAAGAG ATATGAGCTC AGGGTGATAA TATGGAATAC TGACGAGGTA 
    ATTCTGGAAG ACGATGATTA CTTCACAGGG GAAAAGTCCA GTGACATTTT TGTCAGGGGC 
    TGGCTGAAAG GACAGCAGGA AGATAAGCAG GACACAGACG TTCACTACCA CTCTCTGACA 
    GGAGAGGGAA ACTTCAACTG GCGCTTTGTG TTTCCCTTCG ACTATCTCAT GGCTGAGGAG 
    AAAATTGTGA TCTCTAAGAA AGAGTCTTTG TTCTCCTGGG ACGAGACAGA GTACAAGATC 
    CCTGCCCGGC TCACCATGCA AGTGTGGGAC GCTGACCACT TCTCTGCTGA TGACTTCTTG 
    GGTGCGATTG AGCTGGACTT GAACCGGTTT CCTCGGGGAG CAAAAACAGC AAAGCAGTGC 
    TCCATCGACA TGGTGCTTAA TGAGCAAGAG CTGCCAACCA TCTCAATCTT CAAACAGAAA 
    AGGGTGAAGG GCTGGTGGCC TTTTGTGGCG CGAGATGAGA ATGATGACCT GGAGCTGACG 
    GGAAAAGTGG AGGCAGAGCT TCATCTAATG ACAGCAGAGG AAGCAGAGAA GAGTCCTGTG 
    GGCCTTGGAA GGAATGAACC AGACCCACTG GAGAAACCAA ATCGCCCGGA CACCAGTCTC 
    ATGTGGTTCA TGAACCCCCT GAAGTCCCTG CGATACTTCA TTTGGCACAA CTACCGCTGG 
    CTGATCCTGA AGACCCTAGG GCTGATGCTG CTCCTCCTGC TCCTCGGCCT CTTTCTCTAC 
    TCCATCCCTG GATACCTGGT CAAGAAGCTG CTGGGGGCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    otof ( XM_007260874.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007260936.1 Astyanax mexicanus otoferlin (otof), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID OAs42397
    Clone ID Related Accession (Same CDS sequence) XM_007260874.2
    Accession Version XM_007260874.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1455033600000
    Organism Astyanax mexicanus(Mexican tetra)
    Product otoferlin isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749495.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007260874.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTTAG TTGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGACGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTCTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCGGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCCTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA GCTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATTGGTCC AGCTGGACGG ACAGAGCTTC CTCCCATTGA TGGGCCAACT 
    GATGTTGACC GAGGTCCCAT CTTACCTGTG CCTCTGGGCA TACGGCCCGT CCTCAGCAGA 
    TACCGCATAG AGGTCTTGTT CTGGGGGCTG CGGGACCTGA AGAGAATTAA CCTGGCGCAG 
    GTCGACAGGC CTCGAGTGGA CATTGAGTGT GCAGGCAAAG GAATCCAATC AGCTCTCATT 
    CAGAATTACA AGAAAAACCC AAATTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGATCTG 
    CCAGAGAACG AGTTGCTTCA TCCTCCTCTG AATATTCGGG TGGTAGACTG CAGAGCATTT 
    GGCCGCTACA CATTGGTGGG CTCACATGCT GTAACCAGTC TGCGCAAGTT CATCTACATC 
    CCTCCTGACA AAAGGTCCAA TAACTGGGCT AATACAGGTG ACATCGTGGT CAACATGGAC 
    TCTGAGCCCA ATGTCAAGAA GATGGAGACT GTTGTAAAAC TGGATGCTAC CTCTGATGCT 
    GTTGTTAAAA TTGATGTGAA TGAGGAAGAA AAGGAAAAAG ACAAAAAGAA GAAAAAGAAA 
    AAGAAGGGAG AGGAGATAGA AGATGAGGAG CCAGATGAGA GCATGCTGGA CTGGTGGTCA 
    AAGTACTTTG CTTCAATAGA GACCATGATG GAGAACCTAC GAGTCCAGGA AGCAGCATGG 
    GCTGAGGCTG AGGAGAGAGA AGATTTGGAG ATTGCGGCAG AGGGAGCAGA AATCAAAAGT 
    GATGACTCTT GTACAAAAGG CACGAAGCCC AAAGAGAAGA ACAAGATAAA GAGCTCAAAG 
    GACAAGAAGA AGAGCCACGG CAGTGATGGG ACAGAGAAAA AAGTGCCCAA ACCCAAAGTA 
    GACGAGCTTT TGGTCTACAA TAAGGAGCTG GAGACTGAGT TTGATCACTT TGAAGACTGG 
    CTTCACACCT TCAACCTGTA CAGAGGGAAG GCCATAGATG ATGATGACCA AAACATTGTG 
    GATGACGACA GGATTGTGGG CCGATTTAAA GGGTCATTGT GCATGTACAA GCTGCCTCTG 
    TCAGATGAAA TCACCAGAGA TGTTGGATTC GATCCAAATA TGGGCATGTT TCAGAGTATC 
    CCACACAATG ATCCCATCAA TGTACTTGTG AGGGTTTATG TTATCAGGGC AACAGACCTT 
    CACCCTGCAG ATATAAACGG AAAAGCTGAT CCATATGTTG TTATCAAGCT GGGAAAGTCA 
    GAGATCAAGG ACAAGGAGAA CTACATCTCT AAACAGCTGA ATCCTGTCTT TGGGAAGTCC 
    TTTGACATTG AAGCCACGTT TCCAATGGAG TCTATGCTGA CAGTCTCAGT GTACGACTGG 
    GATTTAGTTG GCACTGATGA TTTGATCGGA GAGACCAAGA TTGACTTGGA GAACCGTTAC 
    TACAGCAAAC ACAGAGCCAC CTGTGGCATC GCATCCAATT ACTCTCTTCA TGGGTACAAT 
    GTGTGGCGAG ACCCTCAGAA GCCCACCCAG ATACTTGCAA AGTTGTGTAA GGAGGGCAAA 
    CTGGATGGAC CGCATTATGG CCCAGGTGGA AAGGTTAAAG TTGCAAACCG CATCTTTCTC 
    GGGCCAACGG ATATTGAAGA TGAAAATGGT CTAAAGAAGC AAACAGAAGA GCATTTGGCT 
    CTTACTGCAC TGAAACACTG GGAGGAAATT CCTCGAGTGG GCTGCAAACT CATCCCAGAA 
    CATGTAGAAA CCCGACCACT CCTCAACCCA GATAAACCTG GAATAGAGCA GGGTCGGCTT 
    GAGATGTGGG TGGATATGTT CCCAATGGAT GTTCCTTCAC CAGGACCAGC CATAGACATA 
    TCACCACGGA AACCAAAGAG ATATGAGCTC AGGGTGATAA TATGGAATAC TGACGAGGTA 
    ATTCTGGAAG ACGATGATTA CTTCACAGGG GAAAAGTCCA GTGACATTTT TGTCAGGGGC 
    TGGCTGAAAG GACAGCAGGA AGATAAGCAG GACACAGACG TTCACTACCA CTCTCTGACA 
    GGAGAGGGAA ACTTCAACTG GCGCTTTGTG TTTCCCTTCG ACTATCTCAT GGCTGAGGAG 
    AAAATTGTGA TCTCTAAGAA AGAGTCTTTG TTCTCCTGGG ACGAGACAGA GTACAAGATC 
    CCTGCCCGGC TCACCATGCA AGTGTGGGAC GCTGACCACT TCTCTGCTGA TGACTTCTTG 
    GGTGCGATTG AGCTGGACTT GAACCGGTTT CCTCGGGGAG CAAAAACAGC AAAGCAGTGC 
    TCCATCGACA TGGTGCTTAA TGAGCAAGAG CTGCCAACCA TCTCAATCTT CAAACAGAAA 
    AGGGTGAAGG GCTGGTGGCC TTTTGTGGCG CGAGATGAGA ATGATGACCT GGAGCTGACG 
    GGAAAAGTGG AGGCAGAGCT TCATCTAATG ACAGCAGAGG AAGCAGAGAA GAGTCCTGTG 
    GGCCTTGGAA GGAATGAACC AGACCCACTG GAGAAACCAA ACCGGCCCGA CACCACCTTC 
    CTCTGGTTCC TGAGTCCACT GAAGGCCATC CGCTACCTGG TGTGCAACCG CTACAAATGG 
    CTCATAATCA AGATTGTCCT GGCCCTGCTG CTGCTGATCA TGGTGGGCCT CTTCCTCTAC 
    AGCATGCCTG GCTACCTGGT CAAGAAGCTG CTGGGAGCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_007260936.1
    CDS134..6115
    Translation

    Target ORF information:

    RefSeq Version XM_007260874.2
    Organism Astyanax mexicanus(Mexican tetra)
    Definition Astyanax mexicanus otoferlin (otof), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_007260874.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTTAG TTGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGACGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTCTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCGGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCCTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA GCTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATTGGTCC AGCTGGACGG ACAGAGCTTC CTCCCATTGA TGGGCCAACT 
    GATGTTGACC GAGGTCCCAT CTTACCTGTG CCTCTGGGCA TACGGCCCGT CCTCAGCAGA 
    TACCGCATAG AGGTCTTGTT CTGGGGGCTG CGGGACCTGA AGAGAATTAA CCTGGCGCAG 
    GTCGACAGGC CTCGAGTGGA CATTGAGTGT GCAGGCAAAG GAATCCAATC AGCTCTCATT 
    CAGAATTACA AGAAAAACCC AAATTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGATCTG 
    CCAGAGAACG AGTTGCTTCA TCCTCCTCTG AATATTCGGG TGGTAGACTG CAGAGCATTT 
    GGCCGCTACA CATTGGTGGG CTCACATGCT GTAACCAGTC TGCGCAAGTT CATCTACATC 
    CCTCCTGACA AAAGGTCCAA TAACTGGGCT AATACAGGTG ACATCGTGGT CAACATGGAC 
    TCTGAGCCCA ATGTCAAGAA GATGGAGACT GTTGTAAAAC TGGATGCTAC CTCTGATGCT 
    GTTGTTAAAA TTGATGTGAA TGAGGAAGAA AAGGAAAAAG ACAAAAAGAA GAAAAAGAAA 
    AAGAAGGGAG AGGAGATAGA AGATGAGGAG CCAGATGAGA GCATGCTGGA CTGGTGGTCA 
    AAGTACTTTG CTTCAATAGA GACCATGATG GAGAACCTAC GAGTCCAGGA AGCAGCATGG 
    GCTGAGGCTG AGGAGAGAGA AGATTTGGAG ATTGCGGCAG AGGGAGCAGA AATCAAAAGT 
    GATGACTCTT GTACAAAAGG CACGAAGCCC AAAGAGAAGA ACAAGATAAA GAGCTCAAAG 
    GACAAGAAGA AGAGCCACGG CAGTGATGGG ACAGAGAAAA AAGTGCCCAA ACCCAAAGTA 
    GACGAGCTTT TGGTCTACAA TAAGGAGCTG GAGACTGAGT TTGATCACTT TGAAGACTGG 
    CTTCACACCT TCAACCTGTA CAGAGGGAAG GCCATAGATG ATGATGACCA AAACATTGTG 
    GATGACGACA GGATTGTGGG CCGATTTAAA GGGTCATTGT GCATGTACAA GCTGCCTCTG 
    TCAGATGAAA TCACCAGAGA TGTTGGATTC GATCCAAATA TGGGCATGTT TCAGAGTATC 
    CCACACAATG ATCCCATCAA TGTACTTGTG AGGGTTTATG TTATCAGGGC AACAGACCTT 
    CACCCTGCAG ATATAAACGG AAAAGCTGAT CCATATGTTG TTATCAAGCT GGGAAAGTCA 
    GAGATCAAGG ACAAGGAGAA CTACATCTCT AAACAGCTGA ATCCTGTCTT TGGGAAGTCC 
    TTTGACATTG AAGCCACGTT TCCAATGGAG TCTATGCTGA CAGTCTCAGT GTACGACTGG 
    GATTTAGTTG GCACTGATGA TTTGATCGGA GAGACCAAGA TTGACTTGGA GAACCGTTAC 
    TACAGCAAAC ACAGAGCCAC CTGTGGCATC GCATCCAATT ACTCTCTTCA TGGGTACAAT 
    GTGTGGCGAG ACCCTCAGAA GCCCACCCAG ATACTTGCAA AGTTGTGTAA GGAGGGCAAA 
    CTGGATGGAC CGCATTATGG CCCAGGTGGA AAGGTTAAAG TTGCAAACCG CATCTTTCTC 
    GGGCCAACGG ATATTGAAGA TGAAAATGGT CTAAAGAAGC AAACAGAAGA GCATTTGGCT 
    CTTACTGCAC TGAAACACTG GGAGGAAATT CCTCGAGTGG GCTGCAAACT CATCCCAGAA 
    CATGTAGAAA CCCGACCACT CCTCAACCCA GATAAACCTG GAATAGAGCA GGGTCGGCTT 
    GAGATGTGGG TGGATATGTT CCCAATGGAT GTTCCTTCAC CAGGACCAGC CATAGACATA 
    TCACCACGGA AACCAAAGAG ATATGAGCTC AGGGTGATAA TATGGAATAC TGACGAGGTA 
    ATTCTGGAAG ACGATGATTA CTTCACAGGG GAAAAGTCCA GTGACATTTT TGTCAGGGGC 
    TGGCTGAAAG GACAGCAGGA AGATAAGCAG GACACAGACG TTCACTACCA CTCTCTGACA 
    GGAGAGGGAA ACTTCAACTG GCGCTTTGTG TTTCCCTTCG ACTATCTCAT GGCTGAGGAG 
    AAAATTGTGA TCTCTAAGAA AGAGTCTTTG TTCTCCTGGG ACGAGACAGA GTACAAGATC 
    CCTGCCCGGC TCACCATGCA AGTGTGGGAC GCTGACCACT TCTCTGCTGA TGACTTCTTG 
    GGTGCGATTG AGCTGGACTT GAACCGGTTT CCTCGGGGAG CAAAAACAGC AAAGCAGTGC 
    TCCATCGACA TGGTGCTTAA TGAGCAAGAG CTGCCAACCA TCTCAATCTT CAAACAGAAA 
    AGGGTGAAGG GCTGGTGGCC TTTTGTGGCG CGAGATGAGA ATGATGACCT GGAGCTGACG 
    GGAAAAGTGG AGGCAGAGCT TCATCTAATG ACAGCAGAGG AAGCAGAGAA GAGTCCTGTG 
    GGCCTTGGAA GGAATGAACC AGACCCACTG GAGAAACCAA ACCGGCCCGA CACCACCTTC 
    CTCTGGTTCC TGAGTCCACT GAAGGCCATC CGCTACCTGG TGTGCAACCG CTACAAATGG 
    CTCATAATCA AGATTGTCCT GGCCCTGCTG CTGCTGATCA TGGTGGGCCT CTTCCTCTAC 
    AGCATGCCTG GCTACCTGGT CAAGAAGCTG CTGGGAGCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    otof ( XM_015608738.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_015464224.2 Astyanax mexicanus otoferlin (otof), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OAs123558
    Clone ID Related Accession (Same CDS sequence) XM_015608738.2
    Accession Version XM_015608738.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6144bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1506873600000
    Organism Astyanax mexicanus(Mexican tetra)
    Product otoferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035899.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 3, 2017 this sequence version replaced XM_015608738.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGATGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTGTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCTGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCTTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA ACTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATCCCTTA CGACGATGAG GAGATCAGGA GGGCTCTTAT TGCTGTCCAT 
    GACTTTGCTG TCCCTCAGAT CAAGATTGGT CCAGCTGGAC GGACAGAGCT TCCTCCCATT 
    GATGGGCCAA CTGATGTTGA CCGAGGTCCC ATCTTACCTG TGCCTCTGGG CATACGGCCC 
    GTCCTCAGCA GATACCGCAT AGAGGTCTTG TTCTGGGGGC TGCGGGACCT GAAGAGAATT 
    AACCTGGCGC AGGTCGACAG GCCTCGAGTG GACATTGAGT GTGCAGGCAA AGGAATCCAA 
    TCAGCTCTCA ATCAGAATTA CAAGAAAAAC CCAAATTTCA GCACCCTGGT CAAGTGGTTT 
    GAAGTGGATC TGCCAGAGAA CGAGTTGCTT CATCCTCCTC TGAATATTCG GGTGGTAGAC 
    TGCAGAGCAT TTGGCCGCTA CACATTGGTG GGCTCACATG CTGTAACCAG TCTGCGCAAG 
    TTCATCTACA TCCCTCCTGA CAAAAGGTCC AATAACTGGG CTAATACAGC TAGACTGGCC 
    AATGGCTACT TGGCTCTCAC CAATGGGACC TCCCGTTCCC GCCCCTACTC CCGCCCCATC 
    TCTCACTCCT CAGGTGACAT CGTGGTCAAC ATGGACTCTG AGCCCAATGT CAAGAAGATG 
    GAGACTGTTG TAAAACTGGA TGCTACCTCT GATGCTGTTG TTAAAATTGA TGTGAATGAG 
    GAAGAAAAGG AAAAAGACAA AAAGAAGAAA AAGAAAAAGA AGGGAGAGGA GATAGAAGAT 
    GAGGAGCCAG ATGAGAGCAT GCTGGACTGG TGGTCAAAGT ACTTTGCTTC AATAGAGACC 
    ATGATGGAGA ACCTACGAGT CCAGGAAGCA GCATGGGCTG AGGCTGAGGA GAGAGAAGAT 
    TTGGAGATTG CGGCAGAGGG AGCAGAAATC AAAAGTGATG ACTCTTGTAC AAAAGGCACG 
    AAGCCCAAAG AGAAGAACAA GATAAAGAGC TCAAAGGACA AGAAGAAGAG CCACGGCAGT 
    GATGGGACAG AGAAAAAAGT GCCCAAACCC AAAGTAGATG AGCTTTTGGT CTACAATAAG 
    GAGCTGGAGA CTGAGTTTGA TCACTTTGAA GACTGGCTTC ACACCTTCAA CCTGTACAGA 
    GGGAAGGCCA TAGATGATGA CGACCAAAAC ATTGTGGATG ACGACAGGAT TGTGGGCCGA 
    TTTAAAGGGT CATTGTGCAT GTACAAGCTG CCTCTGTCAG ATGAAATCAC CAGAGATGTT 
    GGATTCGATC CAAATATGGG CATGTTTCAG AGTATCCCAC ACAATGATCC CATCAATGTA 
    CTTGTGAGGG TTTATGTTAT CAGGGCAACA GACCTTCACC CTGCAGATAT AAACGGAAAA 
    GCTGATCCAT ATGTTGTTAT CAAGCTGGGA AAGTCAGAGA TCAAGGACAA GGAGAACTAC 
    ATCTCTAAAC AGCTGAATCC TGTCTTTGGG AAGTCCTTTG ACATTGAAGC CACGTTTCCA 
    ATGGAGTCTA TGCTGACAGT CTCAGTGTAC GACTGGGATT TAGTTGGCAC TGATGATTTG 
    ATCGGAGAGA CCAAGATTGA CTTGGAGAAC CGTTACTACA GCAAACACAG AGCCACCTGT 
    GGCATCGCAT CCAATTACTC TCTTCATGGG TACAATGTGT GGCGAGACCC TCAGAAGCCC 
    ACCCAGATAC TTGCAAAGTT GTGTAAGGAG GGCAAACTGG ATGGACCGCA TTATGGCCCA 
    GGTGGAAAGG TTAAAGTTGC AAACCGCATC TTTCTCGGGC CAACGGATAT TGAAGATGAA 
    AATGGTCTAA AGAAGCAAAC AGAAGAGCAT TTGGCTCTTA CTGCACTGAA ACACTGGGAG 
    GAAATTCCTC GAGTGGGCTG CAAACTCATC CCAGAACATG TAGAAACCCG ACCACTCCTC 
    AACCCAGATA AACCTGGAAT AGAGCAGGGT CGGCTTGAGA TGTGGGTGGA TATGTTCCCA 
    ATGGATGTTC CTTCACCAGG ACCAGCCATA GACATATCAC CACGGAAACC AAAGAGATAT 
    GAGCTCAGGG TGATAATATG GAATACTGAC GAGGTAATTC TGGAAGACGA TGATTACTTC 
    ACAGGGGAAA AGTCCAGTGA CATTTTTGTC AGGGGCTGGC TGAAAGGACA GCAGGAAGAT 
    AAGCAGGACA CAGACGTTCA CTACCACTCT CTGACAGGAG AGGGAAACTT CAACTGGCGC 
    TTTGTGTTTC CCTTCGACTA TCTCATGGCT GAGGAGAAAA TTGTGATCTC TAAGAAAGAG 
    TCTTTGTTCT CCTGGGACGA GACAGAGTAC AAGATCCCTG CCCGGCTCAC CATGCAAGTG 
    TGGGACGCTG ACCACTTCTC TGCTGATGAC TTCTTGGGTG CGATTGAGCT GGACTTGAAC 
    CGGTTTCCTC GGGGAGCAAA AACAGCAAAG CAGTGCTCCA TCGACATGGT GCTTAATGAG 
    CAAGAGCTGC CAACCATCTC AATCTTCAAA CAGAAAAGGG TGAAGGGCTG GTGGCCTTTT 
    GTGGCGCGAG ATGAGAATGA TGACCTGGAG CTGACGGGAA AAGTGGAGGC AGAGCTTCAT 
    CTAATGACAG CGGAGGAAGC AGAGAAGAGT CCTGTGGGCC TTGGAAGGAA TGAACCAGAC 
    CCACTGGAGA AACCAAACCG TCCCAACACC AGCTTGTCGT GGTTGTTGAG TCCGATGCGA 
    GTGGCGTGTA TCCTGGCATG GAGGCAGTAT AAGATGCCGT GTTTGGTGCT GCTTCTCTGT 
    ACAGCAGGCC TGATATTCCT GGGCCTGCTC ATCTTCTCCA TTCCTTCTGC TCTCAGTCAG 
    AAGATGGTCA GTTTTCTAGG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015464224.2
    CDS268..6411
    Translation

    Target ORF information:

    RefSeq Version XM_015608738.2
    Organism Astyanax mexicanus(Mexican tetra)
    Definition Astyanax mexicanus otoferlin (otof), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_015608738.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGATGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTGTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCTGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCTTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA ACTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATCCCTTA CGACGATGAG GAGATCAGGA GGGCTCTTAT TGCTGTCCAT 
    GACTTTGCTG TCCCTCAGAT CAAGATTGGT CCAGCTGGAC GGACAGAGCT TCCTCCCATT 
    GATGGGCCAA CTGATGTTGA CCGAGGTCCC ATCTTACCTG TGCCTCTGGG CATACGGCCC 
    GTCCTCAGCA GATACCGCAT AGAGGTCTTG TTCTGGGGGC TGCGGGACCT GAAGAGAATT 
    AACCTGGCGC AGGTCGACAG GCCTCGAGTG GACATTGAGT GTGCAGGCAA AGGAATCCAA 
    TCAGCTCTCA ATCAGAATTA CAAGAAAAAC CCAAATTTCA GCACCCTGGT CAAGTGGTTT 
    GAAGTGGATC TGCCAGAGAA CGAGTTGCTT CATCCTCCTC TGAATATTCG GGTGGTAGAC 
    TGCAGAGCAT TTGGCCGCTA CACATTGGTG GGCTCACATG CTGTAACCAG TCTGCGCAAG 
    TTCATCTACA TCCCTCCTGA CAAAAGGTCC AATAACTGGG CTAATACAGC TAGACTGGCC 
    AATGGCTACT TGGCTCTCAC CAATGGGACC TCCCGTTCCC GCCCCTACTC CCGCCCCATC 
    TCTCACTCCT CAGGTGACAT CGTGGTCAAC ATGGACTCTG AGCCCAATGT CAAGAAGATG 
    GAGACTGTTG TAAAACTGGA TGCTACCTCT GATGCTGTTG TTAAAATTGA TGTGAATGAG 
    GAAGAAAAGG AAAAAGACAA AAAGAAGAAA AAGAAAAAGA AGGGAGAGGA GATAGAAGAT 
    GAGGAGCCAG ATGAGAGCAT GCTGGACTGG TGGTCAAAGT ACTTTGCTTC AATAGAGACC 
    ATGATGGAGA ACCTACGAGT CCAGGAAGCA GCATGGGCTG AGGCTGAGGA GAGAGAAGAT 
    TTGGAGATTG CGGCAGAGGG AGCAGAAATC AAAAGTGATG ACTCTTGTAC AAAAGGCACG 
    AAGCCCAAAG AGAAGAACAA GATAAAGAGC TCAAAGGACA AGAAGAAGAG CCACGGCAGT 
    GATGGGACAG AGAAAAAAGT GCCCAAACCC AAAGTAGATG AGCTTTTGGT CTACAATAAG 
    GAGCTGGAGA CTGAGTTTGA TCACTTTGAA GACTGGCTTC ACACCTTCAA CCTGTACAGA 
    GGGAAGGCCA TAGATGATGA CGACCAAAAC ATTGTGGATG ACGACAGGAT TGTGGGCCGA 
    TTTAAAGGGT CATTGTGCAT GTACAAGCTG CCTCTGTCAG ATGAAATCAC CAGAGATGTT 
    GGATTCGATC CAAATATGGG CATGTTTCAG AGTATCCCAC ACAATGATCC CATCAATGTA 
    CTTGTGAGGG TTTATGTTAT CAGGGCAACA GACCTTCACC CTGCAGATAT AAACGGAAAA 
    GCTGATCCAT ATGTTGTTAT CAAGCTGGGA AAGTCAGAGA TCAAGGACAA GGAGAACTAC 
    ATCTCTAAAC AGCTGAATCC TGTCTTTGGG AAGTCCTTTG ACATTGAAGC CACGTTTCCA 
    ATGGAGTCTA TGCTGACAGT CTCAGTGTAC GACTGGGATT TAGTTGGCAC TGATGATTTG 
    ATCGGAGAGA CCAAGATTGA CTTGGAGAAC CGTTACTACA GCAAACACAG AGCCACCTGT 
    GGCATCGCAT CCAATTACTC TCTTCATGGG TACAATGTGT GGCGAGACCC TCAGAAGCCC 
    ACCCAGATAC TTGCAAAGTT GTGTAAGGAG GGCAAACTGG ATGGACCGCA TTATGGCCCA 
    GGTGGAAAGG TTAAAGTTGC AAACCGCATC TTTCTCGGGC CAACGGATAT TGAAGATGAA 
    AATGGTCTAA AGAAGCAAAC AGAAGAGCAT TTGGCTCTTA CTGCACTGAA ACACTGGGAG 
    GAAATTCCTC GAGTGGGCTG CAAACTCATC CCAGAACATG TAGAAACCCG ACCACTCCTC 
    AACCCAGATA AACCTGGAAT AGAGCAGGGT CGGCTTGAGA TGTGGGTGGA TATGTTCCCA 
    ATGGATGTTC CTTCACCAGG ACCAGCCATA GACATATCAC CACGGAAACC AAAGAGATAT 
    GAGCTCAGGG TGATAATATG GAATACTGAC GAGGTAATTC TGGAAGACGA TGATTACTTC 
    ACAGGGGAAA AGTCCAGTGA CATTTTTGTC AGGGGCTGGC TGAAAGGACA GCAGGAAGAT 
    AAGCAGGACA CAGACGTTCA CTACCACTCT CTGACAGGAG AGGGAAACTT CAACTGGCGC 
    TTTGTGTTTC CCTTCGACTA TCTCATGGCT GAGGAGAAAA TTGTGATCTC TAAGAAAGAG 
    TCTTTGTTCT CCTGGGACGA GACAGAGTAC AAGATCCCTG CCCGGCTCAC CATGCAAGTG 
    TGGGACGCTG ACCACTTCTC TGCTGATGAC TTCTTGGGTG CGATTGAGCT GGACTTGAAC 
    CGGTTTCCTC GGGGAGCAAA AACAGCAAAG CAGTGCTCCA TCGACATGGT GCTTAATGAG 
    CAAGAGCTGC CAACCATCTC AATCTTCAAA CAGAAAAGGG TGAAGGGCTG GTGGCCTTTT 
    GTGGCGCGAG ATGAGAATGA TGACCTGGAG CTGACGGGAA AAGTGGAGGC AGAGCTTCAT 
    CTAATGACAG CGGAGGAAGC AGAGAAGAGT CCTGTGGGCC TTGGAAGGAA TGAACCAGAC 
    CCACTGGAGA AACCAAACCG TCCCAACACC AGCTTGTCGT GGTTGTTGAG TCCGATGCGA 
    GTGGCGTGTA TCCTGGCATG GAGGCAGTAT AAGATGCCGT GTTTGGTGCT GCTTCTCTGT 
    ACAGCAGGCC TGATATTCCT GGGCCTGCTC ATCTTCTCCA TTCCTTCTGC TCTCAGTCAG 
    AAGATGGTCA GTTTTCTAGG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    otof ( XM_022686215.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_022541936.1 Astyanax mexicanus otoferlin (otof), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OAs123559
    Clone ID Related Accession (Same CDS sequence) XM_022686215.1
    Accession Version XM_022686215.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1506873600000
    Organism Astyanax mexicanus(Mexican tetra)
    Product otoferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035899.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGATGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTGTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCTGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCTTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA ACTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATCCCTTA CGACGATGAG GAGATCAGGA GGGCTCTTAT TGCTGTCCAT 
    GACTTTGCTG TCCCTCAGAT CAAGATTGGT CCAGCTGGAC GGACAGAGCT TCCTCCCATT 
    GATGGGCCAA CTGATGTTGA CCGAGGTCCC ATCTTACCTG TGCCTCTGGG CATACGGCCC 
    GTCCTCAGCA GATACCGCAT AGAGGTCTTG TTCTGGGGGC TGCGGGACCT GAAGAGAATT 
    AACCTGGCGC AGGTCGACAG GCCTCGAGTG GACATTGAGT GTGCAGGCAA AGGAATCCAA 
    TCAGCTCTCA ATCAGAATTA CAAGAAAAAC CCAAATTTCA GCACCCTGGT CAAGTGGTTT 
    GAAGTGGATC TGCCAGAGAA CGAGTTGCTT CATCCTCCTC TGAATATTCG GGTGGTAGAC 
    TGCAGAGCAT TTGGCCGCTA CACATTGGTG GGCTCACATG CTGTAACCAG TCTGCGCAAG 
    TTCATCTACA TCCCTCCTGA CAAAAGGTCC AATAACTGGG CTAATACAGC TAGACTGGCC 
    AATGGCTACT TGGCTCTCAC CAATGGGACC TCCCGTTCCC GCCCCTACTC CCGCCCCATC 
    TCTCACTCCT CAGGTGACAT CGTGGTCAAC ATGGACTCTG AGCCCAATGT CAAGAAGATG 
    GAGACTGTTG TAAAACTGGA TGCTACCTCT GATGCTGTTG TTAAAATTGA TGTGAATGAG 
    GAAGAAAAGG AAAAAGACAA AAAGAAGAAA AAGAAAAAGA AGGGAGAGGA GATAGAAGAT 
    GAGGAGCCAG ATGAGAGCAT GCTGGACTGG TGGTCAAAGT ACTTTGCTTC AATAGAGACC 
    ATGATGGAGA ACCTACGAGT CCAGGAAGCA GCATGGGCTG AGGCTGAGGA GAGAGAAGAT 
    TTGGAGATTG CGGCAGAGGG AGCAGAAATC AAAAGTGATG ACTCTTGTAC AAAAGGCACG 
    AAGCCCAAAG AGAAGAACAA GATAAAGAGC TCAAAGGACA AGAAGAAGAG CCACGGCAGT 
    GATGGGACAG AGAAAAAAGT GCCCAAACCC AAAGTAGATG AGCTTTTGGT CTACAATAAG 
    GAGCTGGAGA CTGAGTTTGA TCACTTTGAA GACTGGCTTC ACACCTTCAA CCTGTACAGA 
    GGGAAGGCCA TAGATGATGA CGACCAAAAC ATTGTGGATG ACGACAGGAT TGTGGGCCGA 
    TTTAAAGGGT CATTGTGCAT GTACAAGCTG CCTCTGTCAG ATGAAATCAC CAGAGATGTT 
    GGATTCGATC CAAATATGGG CATGTTTCAG AGTATCCCAC ACAATGATCC CATCAATGTA 
    CTTGTGAGGG TTTATGTTAT CAGGGCAACA GACCTTCACC CTGCAGATAT AAACGGAAAA 
    GCTGATCCAT ATGTTGTTAT CAAGCTGGGA AAGTCAGAGA TCAAGGACAA GGAGAACTAC 
    ATCTCTAAAC AGCTGAATCC TGTCTTTGGG AAGTCCTTTG ACATTGAAGC CACGTTTCCA 
    ATGGAGTCTA TGCTGACAGT CTCAGTGTAC GACTGGGATT TAGTTGGCAC TGATGATTTG 
    ATCGGAGAGA CCAAGATTGA CTTGGAGAAC CGTTACTACA GCAAACACAG AGCCACCTGT 
    GGCATCGCAT CCAATTACTC TCTTCATGGG TACAATGTGT GGCGAGACCC TCAGAAGCCC 
    ACCCAGATAC TTGCAAAGTT GTGTAAGGAG GGCAAACTGG ATGGACCGCA TTATGGCCCA 
    GGTGGAAAGG TTAAAGTTGC AAACCGCATC TTTCTCGGGC CAACGGATAT TGAAGATGAA 
    AATGGTCTAA AGAAGCAAAC AGAAGAGCAT TTGGCTCTTA CTGCACTGAA ACACTGGGAG 
    GAAATTCCTC GAGTGGGCTG CAAACTCATC CCAGAACATG TAGAAACCCG ACCACTCCTC 
    AACCCAGATA AACCTGGAAT AGAGCAGGGT CGGCTTGAGA TGTGGGTGGA TATGTTCCCA 
    ATGGATGTTC CTTCACCAGG ACCAGCCATA GACATATCAC CACGGAAACC AAAGAGATAT 
    GAGCTCAGGG TGATAATATG GAATACTGAC GAGGTAATTC TGGAAGACGA TGATTACTTC 
    ACAGGGGAAA AGTCCAGTGA CATTTTTGTC AGGGGCTGGC TGAAAGGACA GCAGGAAGAT 
    AAGCAGGACA CAGACGTTCA CTACCACTCT CTGACAGGAG AGGGAAACTT CAACTGGCGC 
    TTTGTGTTTC CCTTCGACTA TCTCATGGCT GAGGAGAAAA TTGTGATCTC TAAGAAAGAG 
    TCTTTGTTCT CCTGGGACGA GACAGAGTAC AAGATCCCTG CCCGGCTCAC CATGCAAGTG 
    TGGGACGCTG ACCACTTCTC TGCTGATGAC TTCTTGGGTG CGATTGAGCT GGACTTGAAC 
    CGGTTTCCTC GGGGAGCAAA AACAGCAAAG CAGTGCTCCA TCGACATGGT GCTTAATGAG 
    CAAGAGCTGC CAACCATCTC AATCTTCAAA CAGAAAAGGG TGAAGGGCTG GTGGCCTTTT 
    GTGGCGCGAG ATGAGAATGA TGACCTGGAG CTGACGGGAA AAGTGGAGGC AGAGCTTCAT 
    CTAATGACAG CGGAGGAAGC AGAGAAGAGT CCTGTGGGCC TTGGAAGGAA TGAACCAGAC 
    CCACTGGAGA AACCAAATCG CCCGGACACC AGTCTCATGT GGTTCATGAA CCCCCTGAAG 
    TCCCTGCGAT ACTTCATTTG GCACAACTAC CGCTGGCTGA TCCTGAAGAC CCTAGGGCTG 
    ATGCTGCTCC TCCTGCTCCT CGGCCTCTTT CTCTACTCCA TCCCTGGATA CCTGGTCAAG 
    AAGCTGCTGG GGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022541936.1
    CDS268..6405
    Translation

    Target ORF information:

    RefSeq Version XM_022686215.1
    Organism Astyanax mexicanus(Mexican tetra)
    Definition Astyanax mexicanus otoferlin (otof), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_022686215.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGATGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTGTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCTGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCTTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA ACTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATCCCTTA CGACGATGAG GAGATCAGGA GGGCTCTTAT TGCTGTCCAT 
    GACTTTGCTG TCCCTCAGAT CAAGATTGGT CCAGCTGGAC GGACAGAGCT TCCTCCCATT 
    GATGGGCCAA CTGATGTTGA CCGAGGTCCC ATCTTACCTG TGCCTCTGGG CATACGGCCC 
    GTCCTCAGCA GATACCGCAT AGAGGTCTTG TTCTGGGGGC TGCGGGACCT GAAGAGAATT 
    AACCTGGCGC AGGTCGACAG GCCTCGAGTG GACATTGAGT GTGCAGGCAA AGGAATCCAA 
    TCAGCTCTCA ATCAGAATTA CAAGAAAAAC CCAAATTTCA GCACCCTGGT CAAGTGGTTT 
    GAAGTGGATC TGCCAGAGAA CGAGTTGCTT CATCCTCCTC TGAATATTCG GGTGGTAGAC 
    TGCAGAGCAT TTGGCCGCTA CACATTGGTG GGCTCACATG CTGTAACCAG TCTGCGCAAG 
    TTCATCTACA TCCCTCCTGA CAAAAGGTCC AATAACTGGG CTAATACAGC TAGACTGGCC 
    AATGGCTACT TGGCTCTCAC CAATGGGACC TCCCGTTCCC GCCCCTACTC CCGCCCCATC 
    TCTCACTCCT CAGGTGACAT CGTGGTCAAC ATGGACTCTG AGCCCAATGT CAAGAAGATG 
    GAGACTGTTG TAAAACTGGA TGCTACCTCT GATGCTGTTG TTAAAATTGA TGTGAATGAG 
    GAAGAAAAGG AAAAAGACAA AAAGAAGAAA AAGAAAAAGA AGGGAGAGGA GATAGAAGAT 
    GAGGAGCCAG ATGAGAGCAT GCTGGACTGG TGGTCAAAGT ACTTTGCTTC AATAGAGACC 
    ATGATGGAGA ACCTACGAGT CCAGGAAGCA GCATGGGCTG AGGCTGAGGA GAGAGAAGAT 
    TTGGAGATTG CGGCAGAGGG AGCAGAAATC AAAAGTGATG ACTCTTGTAC AAAAGGCACG 
    AAGCCCAAAG AGAAGAACAA GATAAAGAGC TCAAAGGACA AGAAGAAGAG CCACGGCAGT 
    GATGGGACAG AGAAAAAAGT GCCCAAACCC AAAGTAGATG AGCTTTTGGT CTACAATAAG 
    GAGCTGGAGA CTGAGTTTGA TCACTTTGAA GACTGGCTTC ACACCTTCAA CCTGTACAGA 
    GGGAAGGCCA TAGATGATGA CGACCAAAAC ATTGTGGATG ACGACAGGAT TGTGGGCCGA 
    TTTAAAGGGT CATTGTGCAT GTACAAGCTG CCTCTGTCAG ATGAAATCAC CAGAGATGTT 
    GGATTCGATC CAAATATGGG CATGTTTCAG AGTATCCCAC ACAATGATCC CATCAATGTA 
    CTTGTGAGGG TTTATGTTAT CAGGGCAACA GACCTTCACC CTGCAGATAT AAACGGAAAA 
    GCTGATCCAT ATGTTGTTAT CAAGCTGGGA AAGTCAGAGA TCAAGGACAA GGAGAACTAC 
    ATCTCTAAAC AGCTGAATCC TGTCTTTGGG AAGTCCTTTG ACATTGAAGC CACGTTTCCA 
    ATGGAGTCTA TGCTGACAGT CTCAGTGTAC GACTGGGATT TAGTTGGCAC TGATGATTTG 
    ATCGGAGAGA CCAAGATTGA CTTGGAGAAC CGTTACTACA GCAAACACAG AGCCACCTGT 
    GGCATCGCAT CCAATTACTC TCTTCATGGG TACAATGTGT GGCGAGACCC TCAGAAGCCC 
    ACCCAGATAC TTGCAAAGTT GTGTAAGGAG GGCAAACTGG ATGGACCGCA TTATGGCCCA 
    GGTGGAAAGG TTAAAGTTGC AAACCGCATC TTTCTCGGGC CAACGGATAT TGAAGATGAA 
    AATGGTCTAA AGAAGCAAAC AGAAGAGCAT TTGGCTCTTA CTGCACTGAA ACACTGGGAG 
    GAAATTCCTC GAGTGGGCTG CAAACTCATC CCAGAACATG TAGAAACCCG ACCACTCCTC 
    AACCCAGATA AACCTGGAAT AGAGCAGGGT CGGCTTGAGA TGTGGGTGGA TATGTTCCCA 
    ATGGATGTTC CTTCACCAGG ACCAGCCATA GACATATCAC CACGGAAACC AAAGAGATAT 
    GAGCTCAGGG TGATAATATG GAATACTGAC GAGGTAATTC TGGAAGACGA TGATTACTTC 
    ACAGGGGAAA AGTCCAGTGA CATTTTTGTC AGGGGCTGGC TGAAAGGACA GCAGGAAGAT 
    AAGCAGGACA CAGACGTTCA CTACCACTCT CTGACAGGAG AGGGAAACTT CAACTGGCGC 
    TTTGTGTTTC CCTTCGACTA TCTCATGGCT GAGGAGAAAA TTGTGATCTC TAAGAAAGAG 
    TCTTTGTTCT CCTGGGACGA GACAGAGTAC AAGATCCCTG CCCGGCTCAC CATGCAAGTG 
    TGGGACGCTG ACCACTTCTC TGCTGATGAC TTCTTGGGTG CGATTGAGCT GGACTTGAAC 
    CGGTTTCCTC GGGGAGCAAA AACAGCAAAG CAGTGCTCCA TCGACATGGT GCTTAATGAG 
    CAAGAGCTGC CAACCATCTC AATCTTCAAA CAGAAAAGGG TGAAGGGCTG GTGGCCTTTT 
    GTGGCGCGAG ATGAGAATGA TGACCTGGAG CTGACGGGAA AAGTGGAGGC AGAGCTTCAT 
    CTAATGACAG CGGAGGAAGC AGAGAAGAGT CCTGTGGGCC TTGGAAGGAA TGAACCAGAC 
    CCACTGGAGA AACCAAATCG CCCGGACACC AGTCTCATGT GGTTCATGAA CCCCCTGAAG 
    TCCCTGCGAT ACTTCATTTG GCACAACTAC CGCTGGCTGA TCCTGAAGAC CCTAGGGCTG 
    ATGCTGCTCC TCCTGCTCCT CGGCCTCTTT CTCTACTCCA TCCCTGGATA CCTGGTCAAG 
    AAGCTGCTGG GGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    otof ( XM_022686222.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_022541943.1 Astyanax mexicanus otoferlin (otof), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OAs123560
    Clone ID Related Accession (Same CDS sequence) XM_022686222.1
    Accession Version XM_022686222.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1506873600000
    Organism Astyanax mexicanus(Mexican tetra)
    Product otoferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035899.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGATGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTGTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCTGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCTTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA ACTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATCCCTTA CGACGATGAG GAGATCAGGA GGGCTCTTAT TGCTGTCCAT 
    GACTTTGCTG TCCCTCAGAT CAAGATTGGT CCAGCTGGAC GGACAGAGCT TCCTCCCATT 
    GATGGGCCAA CTGATGTTGA CCGAGGTCCC ATCTTACCTG TGCCTCTGGG CATACGGCCC 
    GTCCTCAGCA GATACCGCAT AGAGGTCTTG TTCTGGGGGC TGCGGGACCT GAAGAGAATT 
    AACCTGGCGC AGGTCGACAG GCCTCGAGTG GACATTGAGT GTGCAGGCAA AGGAATCCAA 
    TCAGCTCTCA ATCAGAATTA CAAGAAAAAC CCAAATTTCA GCACCCTGGT CAAGTGGTTT 
    GAAGTGGATC TGCCAGAGAA CGAGTTGCTT CATCCTCCTC TGAATATTCG GGTGGTAGAC 
    TGCAGAGCAT TTGGCCGCTA CACATTGGTG GGCTCACATG CTGTAACCAG TCTGCGCAAG 
    TTCATCTACA TCCCTCCTGA CAAAAGGTCC AATAACTGGG CTAATACAGC TAGACTGGCC 
    AATGGCTACT TGGCTCTCAC CAATGGGACC TCCCGTTCCC GCCCCTACTC CCGCCCCATC 
    TCTCACTCCT CAGGTGACAT CGTGGTCAAC ATGGACTCTG AGCCCAATGT CAAGAAGATG 
    GAGACTGTTG TAAAACTGGA TGCTACCTCT GATGCTGTTG TTAAAATTGA TGTGAATGAG 
    GAAGAAAAGG AAAAAGACAA AAAGAAGAAA AAGAAAAAGA AGGGAGAGGA GATAGAAGAT 
    GAGGAGCCAG ATGAGAGCAT GCTGGACTGG TGGTCAAAGT ACTTTGCTTC AATAGAGACC 
    ATGATGGAGA ACCTACGAGT CCAGGAAGCA GCATGGGCTG AGGCTGAGGA GAGAGAAGAT 
    TTGGAGATTG CGGCAGAGGG AGCAGAAATC AAAAGTGATG ACTCTTGTAC AAAAGGCACG 
    AAGCCCAAAG AGAAGAACAA GATAAAGAGC TCAAAGGACA AGAAGAAGAG CCACGGCAGT 
    GATGGGACAG AGAAAAAAGT GCCCAAACCC AAAGTAGATG AGCTTTTGGT CTACAATAAG 
    GAGCTGGAGA CTGAGTTTGA TCACTTTGAA GACTGGCTTC ACACCTTCAA CCTGTACAGA 
    GGGAAGGCCA TAGATGATGA CGACCAAAAC ATTGTGGATG ACGACAGGAT TGTGGGCCGA 
    TTTAAAGGGT CATTGTGCAT GTACAAGCTG CCTCTGTCAG ATGAAATCAC CAGAGATGTT 
    GGATTCGATC CAAATATGGG CATGTTTCAG AGTATCCCAC ACAATGATCC CATCAATGTA 
    CTTGTGAGGG TTTATGTTAT CAGGGCAACA GACCTTCACC CTGCAGATAT AAACGGAAAA 
    GCTGATCCAT ATGTTGTTAT CAAGCTGGGA AAGTCAGAGA TCAAGGACAA GGAGAACTAC 
    ATCTCTAAAC AGCTGAATCC TGTCTTTGGG AAGTCCTTTG ACATTGAAGC CACGTTTCCA 
    ATGGAGTCTA TGCTGACAGT CTCAGTGTAC GACTGGGATT TAGTTGGCAC TGATGATTTG 
    ATCGGAGAGA CCAAGATTGA CTTGGAGAAC CGTTACTACA GCAAACACAG AGCCACCTGT 
    GGCATCGCAT CCAATTACTC TCTTCATGGG TACAATGTGT GGCGAGACCC TCAGAAGCCC 
    ACCCAGATAC TTGCAAAGTT GTGTAAGGAG GGCAAACTGG ATGGACCGCA TTATGGCCCA 
    GGTGGAAAGG TTAAAGTTGC AAACCGCATC TTTCTCGGGC CAACGGATAT TGAAGATGAA 
    AATGGTCTAA AGAAGCAAAC AGAAGAGCAT TTGGCTCTTA CTGCACTGAA ACACTGGGAG 
    GAAATTCCTC GAGTGGGCTG CAAACTCATC CCAGAACATG TAGAAACCCG ACCACTCCTC 
    AACCCAGATA AACCTGGAAT AGAGCAGGGT CGGCTTGAGA TGTGGGTGGA TATGTTCCCA 
    ATGGATGTTC CTTCACCAGG ACCAGCCATA GACATATCAC CACGGAAACC AAAGAGCTTT 
    GAGCTTCGTG TTATTATTTG GAACACTGAT GACGTAATTC TAGAAGACGA TGCTTTCATG 
    ACAGGAGAGA AGATGTCTGA CATCTATGTC AGGGGCTGGC TGAAAGGACA GCAGGAAGAT 
    AAGCAGGACA CAGACGTTCA CTACCACTCT CTGACAGGAG AGGGAAACTT CAACTGGCGC 
    TTTGTGTTTC CCTTCGACTA TCTCATGGCT GAGGAGAAAA TTGTGATCTC TAAGAAAGAG 
    TCTTTGTTCT CCTGGGACGA GACAGAGTAC AAGATCCCTG CCCGGCTCAC CATGCAAGTG 
    TGGGACGCTG ACCACTTCTC TGCTGATGAC TTCTTGGGTG CGATTGAGCT GGACTTGAAC 
    CGGTTTCCTC GGGGAGCAAA AACAGCAAAG CAGTGCTCCA TCGACATGGT GCTTAATGAG 
    CAAGAGCTGC CAACCATCTC AATCTTCAAA CAGAAAAGGG TGAAGGGCTG GTGGCCTTTT 
    GTGGCGCGAG ATGAGAATGA TGACCTGGAG CTGACGGGAA AAGTGGAGGC AGAGCTTCAT 
    CTAATGACAG CGGAGGAAGC AGAGAAGAGT CCTGTGGGCC TTGGAAGGAA TGAACCAGAC 
    CCACTGGAGA AACCAAATCG CCCGGACACC AGTCTCATGT GGTTCATGAA CCCCCTGAAG 
    TCCCTGCGAT ACTTCATTTG GCACAACTAC CGCTGGCTGA TCCTGAAGAC CCTAGGGCTG 
    ATGCTGCTCC TCCTGCTCCT CGGCCTCTTT CTCTACTCCA TCCCTGGATA CCTGGTCAAG 
    AAGCTGCTGG GGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022541943.1
    CDS268..6405
    Translation

    Target ORF information:

    RefSeq Version XM_022686222.1
    Organism Astyanax mexicanus(Mexican tetra)
    Definition Astyanax mexicanus otoferlin (otof), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_022686222.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGATGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTGTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCTGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCTTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA ACTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATCCCTTA CGACGATGAG GAGATCAGGA GGGCTCTTAT TGCTGTCCAT 
    GACTTTGCTG TCCCTCAGAT CAAGATTGGT CCAGCTGGAC GGACAGAGCT TCCTCCCATT 
    GATGGGCCAA CTGATGTTGA CCGAGGTCCC ATCTTACCTG TGCCTCTGGG CATACGGCCC 
    GTCCTCAGCA GATACCGCAT AGAGGTCTTG TTCTGGGGGC TGCGGGACCT GAAGAGAATT 
    AACCTGGCGC AGGTCGACAG GCCTCGAGTG GACATTGAGT GTGCAGGCAA AGGAATCCAA 
    TCAGCTCTCA ATCAGAATTA CAAGAAAAAC CCAAATTTCA GCACCCTGGT CAAGTGGTTT 
    GAAGTGGATC TGCCAGAGAA CGAGTTGCTT CATCCTCCTC TGAATATTCG GGTGGTAGAC 
    TGCAGAGCAT TTGGCCGCTA CACATTGGTG GGCTCACATG CTGTAACCAG TCTGCGCAAG 
    TTCATCTACA TCCCTCCTGA CAAAAGGTCC AATAACTGGG CTAATACAGC TAGACTGGCC 
    AATGGCTACT TGGCTCTCAC CAATGGGACC TCCCGTTCCC GCCCCTACTC CCGCCCCATC 
    TCTCACTCCT CAGGTGACAT CGTGGTCAAC ATGGACTCTG AGCCCAATGT CAAGAAGATG 
    GAGACTGTTG TAAAACTGGA TGCTACCTCT GATGCTGTTG TTAAAATTGA TGTGAATGAG 
    GAAGAAAAGG AAAAAGACAA AAAGAAGAAA AAGAAAAAGA AGGGAGAGGA GATAGAAGAT 
    GAGGAGCCAG ATGAGAGCAT GCTGGACTGG TGGTCAAAGT ACTTTGCTTC AATAGAGACC 
    ATGATGGAGA ACCTACGAGT CCAGGAAGCA GCATGGGCTG AGGCTGAGGA GAGAGAAGAT 
    TTGGAGATTG CGGCAGAGGG AGCAGAAATC AAAAGTGATG ACTCTTGTAC AAAAGGCACG 
    AAGCCCAAAG AGAAGAACAA GATAAAGAGC TCAAAGGACA AGAAGAAGAG CCACGGCAGT 
    GATGGGACAG AGAAAAAAGT GCCCAAACCC AAAGTAGATG AGCTTTTGGT CTACAATAAG 
    GAGCTGGAGA CTGAGTTTGA TCACTTTGAA GACTGGCTTC ACACCTTCAA CCTGTACAGA 
    GGGAAGGCCA TAGATGATGA CGACCAAAAC ATTGTGGATG ACGACAGGAT TGTGGGCCGA 
    TTTAAAGGGT CATTGTGCAT GTACAAGCTG CCTCTGTCAG ATGAAATCAC CAGAGATGTT 
    GGATTCGATC CAAATATGGG CATGTTTCAG AGTATCCCAC ACAATGATCC CATCAATGTA 
    CTTGTGAGGG TTTATGTTAT CAGGGCAACA GACCTTCACC CTGCAGATAT AAACGGAAAA 
    GCTGATCCAT ATGTTGTTAT CAAGCTGGGA AAGTCAGAGA TCAAGGACAA GGAGAACTAC 
    ATCTCTAAAC AGCTGAATCC TGTCTTTGGG AAGTCCTTTG ACATTGAAGC CACGTTTCCA 
    ATGGAGTCTA TGCTGACAGT CTCAGTGTAC GACTGGGATT TAGTTGGCAC TGATGATTTG 
    ATCGGAGAGA CCAAGATTGA CTTGGAGAAC CGTTACTACA GCAAACACAG AGCCACCTGT 
    GGCATCGCAT CCAATTACTC TCTTCATGGG TACAATGTGT GGCGAGACCC TCAGAAGCCC 
    ACCCAGATAC TTGCAAAGTT GTGTAAGGAG GGCAAACTGG ATGGACCGCA TTATGGCCCA 
    GGTGGAAAGG TTAAAGTTGC AAACCGCATC TTTCTCGGGC CAACGGATAT TGAAGATGAA 
    AATGGTCTAA AGAAGCAAAC AGAAGAGCAT TTGGCTCTTA CTGCACTGAA ACACTGGGAG 
    GAAATTCCTC GAGTGGGCTG CAAACTCATC CCAGAACATG TAGAAACCCG ACCACTCCTC 
    AACCCAGATA AACCTGGAAT AGAGCAGGGT CGGCTTGAGA TGTGGGTGGA TATGTTCCCA 
    ATGGATGTTC CTTCACCAGG ACCAGCCATA GACATATCAC CACGGAAACC AAAGAGCTTT 
    GAGCTTCGTG TTATTATTTG GAACACTGAT GACGTAATTC TAGAAGACGA TGCTTTCATG 
    ACAGGAGAGA AGATGTCTGA CATCTATGTC AGGGGCTGGC TGAAAGGACA GCAGGAAGAT 
    AAGCAGGACA CAGACGTTCA CTACCACTCT CTGACAGGAG AGGGAAACTT CAACTGGCGC 
    TTTGTGTTTC CCTTCGACTA TCTCATGGCT GAGGAGAAAA TTGTGATCTC TAAGAAAGAG 
    TCTTTGTTCT CCTGGGACGA GACAGAGTAC AAGATCCCTG CCCGGCTCAC CATGCAAGTG 
    TGGGACGCTG ACCACTTCTC TGCTGATGAC TTCTTGGGTG CGATTGAGCT GGACTTGAAC 
    CGGTTTCCTC GGGGAGCAAA AACAGCAAAG CAGTGCTCCA TCGACATGGT GCTTAATGAG 
    CAAGAGCTGC CAACCATCTC AATCTTCAAA CAGAAAAGGG TGAAGGGCTG GTGGCCTTTT 
    GTGGCGCGAG ATGAGAATGA TGACCTGGAG CTGACGGGAA AAGTGGAGGC AGAGCTTCAT 
    CTAATGACAG CGGAGGAAGC AGAGAAGAGT CCTGTGGGCC TTGGAAGGAA TGAACCAGAC 
    CCACTGGAGA AACCAAATCG CCCGGACACC AGTCTCATGT GGTTCATGAA CCCCCTGAAG 
    TCCCTGCGAT ACTTCATTTG GCACAACTAC CGCTGGCTGA TCCTGAAGAC CCTAGGGCTG 
    ATGCTGCTCC TCCTGCTCCT CGGCCTCTTT CTCTACTCCA TCCCTGGATA CCTGGTCAAG 
    AAGCTGCTGG GGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    otof ( XM_022686227.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_022541948.1 Astyanax mexicanus otoferlin (otof), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OAs123561
    Clone ID Related Accession (Same CDS sequence) XM_022686227.1
    Accession Version XM_022686227.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1506873600000
    Organism Astyanax mexicanus(Mexican tetra)
    Product otoferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035899.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGATGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTGTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCTGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCTTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA ACTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATCCCTTA CGACGATGAG GAGATCAGGA GGGCTCTTAT TGCTGTCCAT 
    GACTTTGCTG TCCCTCAGAT CAAGATTGGT CCAGCTGGAC GGACAGAGCT TCCTCCCATT 
    GATGGGCCAA CTGATGTTGA CCGAGGTCCC ATCTTACCTG TGCCTCTGGG CATACGGCCC 
    GTCCTCAGCA GATACCGCAT AGAGGTCTTG TTCTGGGGGC TGCGGGACCT GAAGAGAATT 
    AACCTGGCGC AGGTCGACAG GCCTCGAGTG GACATTGAGT GTGCAGGCAA AGGAATCCAA 
    TCAGCTCTCA ATCAGAATTA CAAGAAAAAC CCAAATTTCA GCACCCTGGT CAAGTGGTTT 
    GAAGTGGATC TGCCAGAGAA CGAGTTGCTT CATCCTCCTC TGAATATTCG GGTGGTAGAC 
    TGCAGAGCAT TTGGCCGCTA CACATTGGTG GGCTCACATG CTGTAACCAG TCTGCGCAAG 
    TTCATCTACA TCCCTCCTGA CAAAAGGTCC AATAACTGGG CTAATACAGC TAGACTGGCC 
    AATGGCTACT TGGCTCTCAC CAATGGGACC TCCCGTTCCC GCCCCTACTC CCGCCCCATC 
    TCTCACTCCT CAGGTGACAT CGTGGTCAAC ATGGACTCTG AGCCCAATGT CAAGAAGATG 
    GAGACTGTTG TAAAACTGGA TGCTACCTCT GATGCTGTTG TTAAAATTGA TGTGAATGAG 
    GAAGAAAAGG AAAAAGACAA AAAGAAGAAA AAGAAAAAGA AGGGAGAGGA GATAGAAGAT 
    GAGGAGCCAG ATGAGAGCAT GCTGGACTGG TGGTCAAAGT ACTTTGCTTC AATAGAGACC 
    ATGATGGAGA ACCTACGAGT CCAGGAAGCA GCATGGGCTG AGGCTGAGGA GAGAGAAGAT 
    TTGGAGATTG CGGCAGAGGG AGCAGAAATC AAAAGTGATG ACTCTTGTAC AAAAGGCACG 
    AAGCCCAAAG AGAAGAACAA GATAAAGAGC TCAAAGGACA AGAAGAAGAG CCACGGCAGT 
    GATGGGACAG AGAAAAAAGT GCCCAAACCC AAAGTAGATG AGCTTTTGGT CTACAATAAG 
    GAGCTGGAGA CTGAGTTTGA TCACTTTGAA GACTGGCTTC ACACCTTCAA CCTGTACAGA 
    GGGAAGGCCA TAGATGATGA CGACCAAAAC ATTGTGGATG ACGACAGGAT TGTGGGCCGA 
    TTTAAAGGGT CATTGTGCAT GTACAAGCTG CCTCTGTCAG ATGAAATCAC CAGAGATGTT 
    GGATTCGATC CAAATATGGG CATGTTTCAG AGTATCCCAC ACAATGATCC CATCAATGTA 
    CTTGTGAGGG TTTATGTTAT CAGGGCAACA GACCTTCACC CTGCAGATAT AAACGGAAAA 
    GCTGATCCAT ATGTTGTTAT CAAGCTGGGA AAGTCAGAGA TCAAGGACAA GGAGAACTAC 
    ATCTCTAAAC AGCTGAATCC TGTCTTTGGG AAGTCCTTTG ACATTGAAGC CACGTTTCCA 
    ATGGAGTCTA TGCTGACAGT CTCAGTGTAC GACTGGGATT TAGTTGGCAC TGATGATTTG 
    ATCGGAGAGA CCAAGATTGA CTTGGAGAAC CGTTACTACA GCAAACACAG AGCCACCTGT 
    GGCATCGCAT CCAATTACTC TCTTCATGGG TACAATGTGT GGCGAGACCC TCAGAAGCCC 
    ACCCAGATAC TTGCAAAGTT GTGTAAGGAG GGCAAACTGG ATGGACCGCA TTATGGCCCA 
    GGTGGAAAGG TTAAAGTTGC AAACCGCATC TTTCTCGGGC CAACGGATAT TGAAGATGAA 
    AATGGTCTAA AGAAGCAAAC AGAAGAGCAT TTGGCTCTTA CTGCACTGAA ACACTGGGAG 
    GAAATTCCTC GAGTGGGCTG CAAACTCATC CCAGAACATG TAGAAACCCG ACCACTCCTC 
    AACCCAGATA AACCTGGAAT AGAGCAGGGT CGGCTTGAGA TGTGGGTGGA TATGTTCCCA 
    ATGGATGTTC CTTCACCAGG ACCAGCCATA GACATATCAC CACGGAAACC AAAGAGATAT 
    GAGCTCAGGG TGATAATATG GAATACTGAC GAGGTAATTC TGGAAGACGA TGATTACTTC 
    ACAGGGGAAA AGTCCAGTGA CATTTTTGTC AGGGGCTGGC TGAAAGGACA GCAGGAAGAT 
    AAGCAGGACA CAGACGTTCA CTACCACTCT CTGACAGGAG AGGGAAACTT CAACTGGCGC 
    TTTGTGTTTC CCTTCGACTA TCTCATGGCT GAGGAGAAAA TTGTGATCTC TAAGAAAGAG 
    TCTTTGTTCT CCTGGGACGA GACAGAGTAC AAGATCCCTG CCCGGCTCAC CATGCAAGTG 
    TGGGACGCTG ACCACTTCTC TGCTGATGAC TTCTTGGGTG CGATTGAGCT GGACTTGAAC 
    CGGTTTCCTC GGGGAGCAAA AACAGCAAAG CAGTGCTCCA TCGACATGGT GCTTAATGAG 
    CAAGAGCTGC CAACCATCTC AATCTTCAAA CAGAAAAGGG TGAAGGGCTG GTGGCCTTTT 
    GTGGCGCGAG ATGAGAATGA TGACCTGGAG CTGACGGGAA AAGTGGAGGC AGAGCTTCAT 
    CTAATGACAG CGGAGGAAGC AGAGAAGAGT CCTGTGGGCC TTGGAAGGAA TGAACCAGAC 
    CCACTGGAGA AACCAAACCG GCCCGACACC ACCTTCCTCT GGTTCCTGAG TCCACTGAAG 
    GCCATCCGCT ACCTGGTGTG CAACCGCTAC AAATGGCTCA TAATCAAGAT TGTCCTGGCC 
    CTGCTGCTGC TGATCATGGT GGGCCTCTTC CTCTACAGCA TGCCTGGCTA CCTGGTCAAG 
    AAGCTGCTGG GAGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022541948.1
    CDS268..6405
    Translation

    Target ORF information:

    RefSeq Version XM_022686227.1
    Organism Astyanax mexicanus(Mexican tetra)
    Definition Astyanax mexicanus otoferlin (otof), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_022686227.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TGGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGATGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTGTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCTGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCTTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA ACTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATCCCTTA CGACGATGAG GAGATCAGGA GGGCTCTTAT TGCTGTCCAT 
    GACTTTGCTG TCCCTCAGAT CAAGATTGGT CCAGCTGGAC GGACAGAGCT TCCTCCCATT 
    GATGGGCCAA CTGATGTTGA CCGAGGTCCC ATCTTACCTG TGCCTCTGGG CATACGGCCC 
    GTCCTCAGCA GATACCGCAT AGAGGTCTTG TTCTGGGGGC TGCGGGACCT GAAGAGAATT 
    AACCTGGCGC AGGTCGACAG GCCTCGAGTG GACATTGAGT GTGCAGGCAA AGGAATCCAA 
    TCAGCTCTCA ATCAGAATTA CAAGAAAAAC CCAAATTTCA GCACCCTGGT CAAGTGGTTT 
    GAAGTGGATC TGCCAGAGAA CGAGTTGCTT CATCCTCCTC TGAATATTCG GGTGGTAGAC 
    TGCAGAGCAT TTGGCCGCTA CACATTGGTG GGCTCACATG CTGTAACCAG TCTGCGCAAG 
    TTCATCTACA TCCCTCCTGA CAAAAGGTCC AATAACTGGG CTAATACAGC TAGACTGGCC 
    AATGGCTACT TGGCTCTCAC CAATGGGACC TCCCGTTCCC GCCCCTACTC CCGCCCCATC 
    TCTCACTCCT CAGGTGACAT CGTGGTCAAC ATGGACTCTG AGCCCAATGT CAAGAAGATG 
    GAGACTGTTG TAAAACTGGA TGCTACCTCT GATGCTGTTG TTAAAATTGA TGTGAATGAG 
    GAAGAAAAGG AAAAAGACAA AAAGAAGAAA AAGAAAAAGA AGGGAGAGGA GATAGAAGAT 
    GAGGAGCCAG ATGAGAGCAT GCTGGACTGG TGGTCAAAGT ACTTTGCTTC AATAGAGACC 
    ATGATGGAGA ACCTACGAGT CCAGGAAGCA GCATGGGCTG AGGCTGAGGA GAGAGAAGAT 
    TTGGAGATTG CGGCAGAGGG AGCAGAAATC AAAAGTGATG ACTCTTGTAC AAAAGGCACG 
    AAGCCCAAAG AGAAGAACAA GATAAAGAGC TCAAAGGACA AGAAGAAGAG CCACGGCAGT 
    GATGGGACAG AGAAAAAAGT GCCCAAACCC AAAGTAGATG AGCTTTTGGT CTACAATAAG 
    GAGCTGGAGA CTGAGTTTGA TCACTTTGAA GACTGGCTTC ACACCTTCAA CCTGTACAGA 
    GGGAAGGCCA TAGATGATGA CGACCAAAAC ATTGTGGATG ACGACAGGAT TGTGGGCCGA 
    TTTAAAGGGT CATTGTGCAT GTACAAGCTG CCTCTGTCAG ATGAAATCAC CAGAGATGTT 
    GGATTCGATC CAAATATGGG CATGTTTCAG AGTATCCCAC ACAATGATCC CATCAATGTA 
    CTTGTGAGGG TTTATGTTAT CAGGGCAACA GACCTTCACC CTGCAGATAT AAACGGAAAA 
    GCTGATCCAT ATGTTGTTAT CAAGCTGGGA AAGTCAGAGA TCAAGGACAA GGAGAACTAC 
    ATCTCTAAAC AGCTGAATCC TGTCTTTGGG AAGTCCTTTG ACATTGAAGC CACGTTTCCA 
    ATGGAGTCTA TGCTGACAGT CTCAGTGTAC GACTGGGATT TAGTTGGCAC TGATGATTTG 
    ATCGGAGAGA CCAAGATTGA CTTGGAGAAC CGTTACTACA GCAAACACAG AGCCACCTGT 
    GGCATCGCAT CCAATTACTC TCTTCATGGG TACAATGTGT GGCGAGACCC TCAGAAGCCC 
    ACCCAGATAC TTGCAAAGTT GTGTAAGGAG GGCAAACTGG ATGGACCGCA TTATGGCCCA 
    GGTGGAAAGG TTAAAGTTGC AAACCGCATC TTTCTCGGGC CAACGGATAT TGAAGATGAA 
    AATGGTCTAA AGAAGCAAAC AGAAGAGCAT TTGGCTCTTA CTGCACTGAA ACACTGGGAG 
    GAAATTCCTC GAGTGGGCTG CAAACTCATC CCAGAACATG TAGAAACCCG ACCACTCCTC 
    AACCCAGATA AACCTGGAAT AGAGCAGGGT CGGCTTGAGA TGTGGGTGGA TATGTTCCCA 
    ATGGATGTTC CTTCACCAGG ACCAGCCATA GACATATCAC CACGGAAACC AAAGAGATAT 
    GAGCTCAGGG TGATAATATG GAATACTGAC GAGGTAATTC TGGAAGACGA TGATTACTTC 
    ACAGGGGAAA AGTCCAGTGA CATTTTTGTC AGGGGCTGGC TGAAAGGACA GCAGGAAGAT 
    AAGCAGGACA CAGACGTTCA CTACCACTCT CTGACAGGAG AGGGAAACTT CAACTGGCGC 
    TTTGTGTTTC CCTTCGACTA TCTCATGGCT GAGGAGAAAA TTGTGATCTC TAAGAAAGAG 
    TCTTTGTTCT CCTGGGACGA GACAGAGTAC AAGATCCCTG CCCGGCTCAC CATGCAAGTG 
    TGGGACGCTG ACCACTTCTC TGCTGATGAC TTCTTGGGTG CGATTGAGCT GGACTTGAAC 
    CGGTTTCCTC GGGGAGCAAA AACAGCAAAG CAGTGCTCCA TCGACATGGT GCTTAATGAG 
    CAAGAGCTGC CAACCATCTC AATCTTCAAA CAGAAAAGGG TGAAGGGCTG GTGGCCTTTT 
    GTGGCGCGAG ATGAGAATGA TGACCTGGAG CTGACGGGAA AAGTGGAGGC AGAGCTTCAT 
    CTAATGACAG CGGAGGAAGC AGAGAAGAGT CCTGTGGGCC TTGGAAGGAA TGAACCAGAC 
    CCACTGGAGA AACCAAACCG GCCCGACACC ACCTTCCTCT GGTTCCTGAG TCCACTGAAG 
    GCCATCCGCT ACCTGGTGTG CAACCGCTAC AAATGGCTCA TAATCAAGAT TGTCCTGGCC 
    CTGCTGCTGC TGATCATGGT GGGCCTCTTC CTCTACAGCA TGCCTGGCTA CCTGGTCAAG 
    AAGCTGCTGG GAGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    otof ( XM_022686236.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_022541957.1 Astyanax mexicanus otoferlin (otof), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID OAs123563
    Clone ID Related Accession (Same CDS sequence) XM_022686236.1
    Accession Version XM_022686236.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6066bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1506873600000
    Organism Astyanax mexicanus(Mexican tetra)
    Product otoferlin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035899.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TGGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGATGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTGTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCTGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCTTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA ACTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATTGGTCC AGCTGGACGG ACAGAGCTTC CTCCCATTGA TGGGCCAACT 
    GATGTTGACC GAGGTCCCAT CTTACCTGTG CCTCTGGGCA TACGGCCCGT CCTCAGCAGA 
    TACCGCATAG AGGTCTTGTT CTGGGGGCTG CGGGACCTGA AGAGAATTAA CCTGGCGCAG 
    GTCGACAGGC CTCGAGTGGA CATTGAGTGT GCAGGCAAAG GAATCCAATC AGCTCTCAAT 
    CAGAATTACA AGAAAAACCC AAATTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGATCTG 
    CCAGAGAACG AGTTGCTTCA TCCTCCTCTG AATATTCGGG TGGTAGACTG CAGAGCATTT 
    GGCCGCTACA CATTGGTGGG CTCACATGCT GTAACCAGTC TGCGCAAGTT CATCTACATC 
    CCTCCTGACA AAAGGTCCAA TAACTGGGCT AATACAGCTA GACTGGCCAA TGGCTACTTG 
    GCTCTCACCA ATGGGACCTC CCGTTCCCGC CCCTACTCCC GCCCCATCTC TCACTCCTCA 
    GGTGACATCG TGGTCAACAT GGACTCTGAG CCCAATGTCA AGAAGATGGA GACTGTTGTA 
    AAACTGGATG CTACCTCTGA TGCTGTTGTT AAAATTGATG TGAATGAGGA AGAAAAGGAA 
    AAAGACAAAA AGAAGAAAAA GAAAAAGAAG GGAGAGGAGA TAGAAGATGA GGAGCCAGAT 
    GAGAGCATGC TGGACTGGTG GTCAAAGTAC TTTGCTTCAA TAGAGACCAT GATGGAGAAC 
    CTACGAGTCC AGGAAGCAGC ATGGGCTGAG GCTGAGGAGA GAGAAGATTT GGAGATTGCG 
    GCAGAGGGAG CAGAAATCAA AAGTGATGAC TCTTGTACAA AAGGCACGAA GCCCAAAGAG 
    AAGAACAAGA TAAAGAGCTC AAAGGACAAG AAGAAGAGCC ACGGCAGTGA TGGGACAGAG 
    AAAAAAGTGC CCAAACCCAA AGTAGATGAG CTTTTGGTCT ACAATAAGGA GCTGGAGACT 
    GAGTTTGATC ACTTTGAAGA CTGGCTTCAC ACCTTCAACC TGTACAGAGG GAAGGCCATA 
    GATGATGACG ACCAAAACAT TGTGGATGAC GACAGGATTG TGGGCCGATT TAAAGGGTCA 
    TTGTGCATGT ACAAGCTGCC TCTGTCAGAT GAAATCACCA GAGATGTTGG ATTCGATCCA 
    AATATGGGCA TGTTTCAGAG TATCCCACAC AATGATCCCA TCAATGTACT TGTGAGGGTT 
    TATGTTATCA GGGCAACAGA CCTTCACCCT GCAGATATAA ACGGAAAAGC TGATCCATAT 
    GTTGTTATCA AGCTGGGAAA GTCAGAGATC AAGGACAAGG AGAACTACAT CTCTAAACAG 
    CTGAATCCTG TCTTTGGGAA GTCCTTTGAC ATTGAAGCCA CGTTTCCAAT GGAGTCTATG 
    CTGACAGTCT CAGTGTACGA CTGGGATTTA GTTGGCACTG ATGATTTGAT CGGAGAGACC 
    AAGATTGACT TGGAGAACCG TTACTACAGC AAACACAGAG CCACCTGTGG CATCGCATCC 
    AATTACTCTC TTCATGGGTA CAATGTGTGG CGAGACCCTC AGAAGCCCAC CCAGATACTT 
    GCAAAGTTGT GTAAGGAGGG CAAACTGGAT GGACCGCATT ATGGCCCAGG TGGAAAGGTT 
    AAAGTTGCAA ACCGCATCTT TCTCGGGCCA ACGGATATTG AAGATGAAAA TGGTCTAAAG 
    AAGCAAACAG AAGAGCATTT GGCTCTTACT GCACTGAAAC ACTGGGAGGA AATTCCTCGA 
    GTGGGCTGCA AACTCATCCC AGAACATGTA GAAACCCGAC CACTCCTCAA CCCAGATAAA 
    CCTGGAATAG AGCAGGGTCG GCTTGAGATG TGGGTGGATA TGTTCCCAAT GGATGTTCCT 
    TCACCAGGAC CAGCCATAGA CATATCACCA CGGAAACCAA AGAGATATGA GCTCAGGGTG 
    ATAATATGGA ATACTGACGA GGTAATTCTG GAAGACGATG ATTACTTCAC AGGGGAAAAG 
    TCCAGTGACA TTTTTGTCAG GGGCTGGCTG AAAGGACAGC AGGAAGATAA GCAGGACACA 
    GACGTTCACT ACCACTCTCT GACAGGAGAG GGAAACTTCA ACTGGCGCTT TGTGTTTCCC 
    TTCGACTATC TCATGGCTGA GGAGAAAATT GTGATCTCTA AGAAAGAGTC TTTGTTCTCC 
    TGGGACGAGA CAGAGTACAA GATCCCTGCC CGGCTCACCA TGCAAGTGTG GGACGCTGAC 
    CACTTCTCTG CTGATGACTT CTTGGGTGCG ATTGAGCTGG ACTTGAACCG GTTTCCTCGG 
    GGAGCAAAAA CAGCAAAGCA GTGCTCCATC GACATGGTGC TTAATGAGCA AGAGCTGCCA 
    ACCATCTCAA TCTTCAAACA GAAAAGGGTG AAGGGCTGGT GGCCTTTTGT GGCGCGAGAT 
    GAGAATGATG ACCTGGAGCT GACGGGAAAA GTGGAGGCAG AGCTTCATCT AATGACAGCG 
    GAGGAAGCAG AGAAGAGTCC TGTGGGCCTT GGAAGGAATG AACCAGACCC ACTGGAGAAA 
    CCAAATCGCC CGGACACCAG TCTCATGTGG TTCATGAACC CCCTGAAGTC CCTGCGATAC 
    TTCATTTGGC ACAACTACCG CTGGCTGATC CTGAAGACCC TAGGGCTGAT GCTGCTCCTC 
    CTGCTCCTCG GCCTCTTTCT CTACTCCATC CCTGGATACC TGGTCAAGAA GCTGCTGGGG 
    GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022541957.1
    CDS268..6333
    Translation

    Target ORF information:

    RefSeq Version XM_022686236.1
    Organism Astyanax mexicanus(Mexican tetra)
    Definition Astyanax mexicanus otoferlin (otof), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_022686236.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TGGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGATGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTGTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCTGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCTTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA ACTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATTGGTCC AGCTGGACGG ACAGAGCTTC CTCCCATTGA TGGGCCAACT 
    GATGTTGACC GAGGTCCCAT CTTACCTGTG CCTCTGGGCA TACGGCCCGT CCTCAGCAGA 
    TACCGCATAG AGGTCTTGTT CTGGGGGCTG CGGGACCTGA AGAGAATTAA CCTGGCGCAG 
    GTCGACAGGC CTCGAGTGGA CATTGAGTGT GCAGGCAAAG GAATCCAATC AGCTCTCAAT 
    CAGAATTACA AGAAAAACCC AAATTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGATCTG 
    CCAGAGAACG AGTTGCTTCA TCCTCCTCTG AATATTCGGG TGGTAGACTG CAGAGCATTT 
    GGCCGCTACA CATTGGTGGG CTCACATGCT GTAACCAGTC TGCGCAAGTT CATCTACATC 
    CCTCCTGACA AAAGGTCCAA TAACTGGGCT AATACAGCTA GACTGGCCAA TGGCTACTTG 
    GCTCTCACCA ATGGGACCTC CCGTTCCCGC CCCTACTCCC GCCCCATCTC TCACTCCTCA 
    GGTGACATCG TGGTCAACAT GGACTCTGAG CCCAATGTCA AGAAGATGGA GACTGTTGTA 
    AAACTGGATG CTACCTCTGA TGCTGTTGTT AAAATTGATG TGAATGAGGA AGAAAAGGAA 
    AAAGACAAAA AGAAGAAAAA GAAAAAGAAG GGAGAGGAGA TAGAAGATGA GGAGCCAGAT 
    GAGAGCATGC TGGACTGGTG GTCAAAGTAC TTTGCTTCAA TAGAGACCAT GATGGAGAAC 
    CTACGAGTCC AGGAAGCAGC ATGGGCTGAG GCTGAGGAGA GAGAAGATTT GGAGATTGCG 
    GCAGAGGGAG CAGAAATCAA AAGTGATGAC TCTTGTACAA AAGGCACGAA GCCCAAAGAG 
    AAGAACAAGA TAAAGAGCTC AAAGGACAAG AAGAAGAGCC ACGGCAGTGA TGGGACAGAG 
    AAAAAAGTGC CCAAACCCAA AGTAGATGAG CTTTTGGTCT ACAATAAGGA GCTGGAGACT 
    GAGTTTGATC ACTTTGAAGA CTGGCTTCAC ACCTTCAACC TGTACAGAGG GAAGGCCATA 
    GATGATGACG ACCAAAACAT TGTGGATGAC GACAGGATTG TGGGCCGATT TAAAGGGTCA 
    TTGTGCATGT ACAAGCTGCC TCTGTCAGAT GAAATCACCA GAGATGTTGG ATTCGATCCA 
    AATATGGGCA TGTTTCAGAG TATCCCACAC AATGATCCCA TCAATGTACT TGTGAGGGTT 
    TATGTTATCA GGGCAACAGA CCTTCACCCT GCAGATATAA ACGGAAAAGC TGATCCATAT 
    GTTGTTATCA AGCTGGGAAA GTCAGAGATC AAGGACAAGG AGAACTACAT CTCTAAACAG 
    CTGAATCCTG TCTTTGGGAA GTCCTTTGAC ATTGAAGCCA CGTTTCCAAT GGAGTCTATG 
    CTGACAGTCT CAGTGTACGA CTGGGATTTA GTTGGCACTG ATGATTTGAT CGGAGAGACC 
    AAGATTGACT TGGAGAACCG TTACTACAGC AAACACAGAG CCACCTGTGG CATCGCATCC 
    AATTACTCTC TTCATGGGTA CAATGTGTGG CGAGACCCTC AGAAGCCCAC CCAGATACTT 
    GCAAAGTTGT GTAAGGAGGG CAAACTGGAT GGACCGCATT ATGGCCCAGG TGGAAAGGTT 
    AAAGTTGCAA ACCGCATCTT TCTCGGGCCA ACGGATATTG AAGATGAAAA TGGTCTAAAG 
    AAGCAAACAG AAGAGCATTT GGCTCTTACT GCACTGAAAC ACTGGGAGGA AATTCCTCGA 
    GTGGGCTGCA AACTCATCCC AGAACATGTA GAAACCCGAC CACTCCTCAA CCCAGATAAA 
    CCTGGAATAG AGCAGGGTCG GCTTGAGATG TGGGTGGATA TGTTCCCAAT GGATGTTCCT 
    TCACCAGGAC CAGCCATAGA CATATCACCA CGGAAACCAA AGAGATATGA GCTCAGGGTG 
    ATAATATGGA ATACTGACGA GGTAATTCTG GAAGACGATG ATTACTTCAC AGGGGAAAAG 
    TCCAGTGACA TTTTTGTCAG GGGCTGGCTG AAAGGACAGC AGGAAGATAA GCAGGACACA 
    GACGTTCACT ACCACTCTCT GACAGGAGAG GGAAACTTCA ACTGGCGCTT TGTGTTTCCC 
    TTCGACTATC TCATGGCTGA GGAGAAAATT GTGATCTCTA AGAAAGAGTC TTTGTTCTCC 
    TGGGACGAGA CAGAGTACAA GATCCCTGCC CGGCTCACCA TGCAAGTGTG GGACGCTGAC 
    CACTTCTCTG CTGATGACTT CTTGGGTGCG ATTGAGCTGG ACTTGAACCG GTTTCCTCGG 
    GGAGCAAAAA CAGCAAAGCA GTGCTCCATC GACATGGTGC TTAATGAGCA AGAGCTGCCA 
    ACCATCTCAA TCTTCAAACA GAAAAGGGTG AAGGGCTGGT GGCCTTTTGT GGCGCGAGAT 
    GAGAATGATG ACCTGGAGCT GACGGGAAAA GTGGAGGCAG AGCTTCATCT AATGACAGCG 
    GAGGAAGCAG AGAAGAGTCC TGTGGGCCTT GGAAGGAATG AACCAGACCC ACTGGAGAAA 
    CCAAATCGCC CGGACACCAG TCTCATGTGG TTCATGAACC CCCTGAAGTC CCTGCGATAC 
    TTCATTTGGC ACAACTACCG CTGGCTGATC CTGAAGACCC TAGGGCTGAT GCTGCTCCTC 
    CTGCTCCTCG GCCTCTTTCT CTACTCCATC CCTGGATACC TGGTCAAGAA GCTGCTGGGG 
    GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    otof ( XM_022686241.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_022541962.1 Astyanax mexicanus otoferlin (otof), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID OAs123564
    Clone ID Related Accession (Same CDS sequence) XM_022686241.1
    Accession Version XM_022686241.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6066bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1506873600000
    Organism Astyanax mexicanus(Mexican tetra)
    Product otoferlin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035899.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TGGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGATGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTGTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCTGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCTTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA ACTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATTGGTCC AGCTGGACGG ACAGAGCTTC CTCCCATTGA TGGGCCAACT 
    GATGTTGACC GAGGTCCCAT CTTACCTGTG CCTCTGGGCA TACGGCCCGT CCTCAGCAGA 
    TACCGCATAG AGGTCTTGTT CTGGGGGCTG CGGGACCTGA AGAGAATTAA CCTGGCGCAG 
    GTCGACAGGC CTCGAGTGGA CATTGAGTGT GCAGGCAAAG GAATCCAATC AGCTCTCAAT 
    CAGAATTACA AGAAAAACCC AAATTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGATCTG 
    CCAGAGAACG AGTTGCTTCA TCCTCCTCTG AATATTCGGG TGGTAGACTG CAGAGCATTT 
    GGCCGCTACA CATTGGTGGG CTCACATGCT GTAACCAGTC TGCGCAAGTT CATCTACATC 
    CCTCCTGACA AAAGGTCCAA TAACTGGGCT AATACAGCTA GACTGGCCAA TGGCTACTTG 
    GCTCTCACCA ATGGGACCTC CCGTTCCCGC CCCTACTCCC GCCCCATCTC TCACTCCTCA 
    GGTGACATCG TGGTCAACAT GGACTCTGAG CCCAATGTCA AGAAGATGGA GACTGTTGTA 
    AAACTGGATG CTACCTCTGA TGCTGTTGTT AAAATTGATG TGAATGAGGA AGAAAAGGAA 
    AAAGACAAAA AGAAGAAAAA GAAAAAGAAG GGAGAGGAGA TAGAAGATGA GGAGCCAGAT 
    GAGAGCATGC TGGACTGGTG GTCAAAGTAC TTTGCTTCAA TAGAGACCAT GATGGAGAAC 
    CTACGAGTCC AGGAAGCAGC ATGGGCTGAG GCTGAGGAGA GAGAAGATTT GGAGATTGCG 
    GCAGAGGGAG CAGAAATCAA AAGTGATGAC TCTTGTACAA AAGGCACGAA GCCCAAAGAG 
    AAGAACAAGA TAAAGAGCTC AAAGGACAAG AAGAAGAGCC ACGGCAGTGA TGGGACAGAG 
    AAAAAAGTGC CCAAACCCAA AGTAGATGAG CTTTTGGTCT ACAATAAGGA GCTGGAGACT 
    GAGTTTGATC ACTTTGAAGA CTGGCTTCAC ACCTTCAACC TGTACAGAGG GAAGGCCATA 
    GATGATGACG ACCAAAACAT TGTGGATGAC GACAGGATTG TGGGCCGATT TAAAGGGTCA 
    TTGTGCATGT ACAAGCTGCC TCTGTCAGAT GAAATCACCA GAGATGTTGG ATTCGATCCA 
    AATATGGGCA TGTTTCAGAG TATCCCACAC AATGATCCCA TCAATGTACT TGTGAGGGTT 
    TATGTTATCA GGGCAACAGA CCTTCACCCT GCAGATATAA ACGGAAAAGC TGATCCATAT 
    GTTGTTATCA AGCTGGGAAA GTCAGAGATC AAGGACAAGG AGAACTACAT CTCTAAACAG 
    CTGAATCCTG TCTTTGGGAA GTCCTTTGAC ATTGAAGCCA CGTTTCCAAT GGAGTCTATG 
    CTGACAGTCT CAGTGTACGA CTGGGATTTA GTTGGCACTG ATGATTTGAT CGGAGAGACC 
    AAGATTGACT TGGAGAACCG TTACTACAGC AAACACAGAG CCACCTGTGG CATCGCATCC 
    AATTACTCTC TTCATGGGTA CAATGTGTGG CGAGACCCTC AGAAGCCCAC CCAGATACTT 
    GCAAAGTTGT GTAAGGAGGG CAAACTGGAT GGACCGCATT ATGGCCCAGG TGGAAAGGTT 
    AAAGTTGCAA ACCGCATCTT TCTCGGGCCA ACGGATATTG AAGATGAAAA TGGTCTAAAG 
    AAGCAAACAG AAGAGCATTT GGCTCTTACT GCACTGAAAC ACTGGGAGGA AATTCCTCGA 
    GTGGGCTGCA AACTCATCCC AGAACATGTA GAAACCCGAC CACTCCTCAA CCCAGATAAA 
    CCTGGAATAG AGCAGGGTCG GCTTGAGATG TGGGTGGATA TGTTCCCAAT GGATGTTCCT 
    TCACCAGGAC CAGCCATAGA CATATCACCA CGGAAACCAA AGAGATATGA GCTCAGGGTG 
    ATAATATGGA ATACTGACGA GGTAATTCTG GAAGACGATG ATTACTTCAC AGGGGAAAAG 
    TCCAGTGACA TTTTTGTCAG GGGCTGGCTG AAAGGACAGC AGGAAGATAA GCAGGACACA 
    GACGTTCACT ACCACTCTCT GACAGGAGAG GGAAACTTCA ACTGGCGCTT TGTGTTTCCC 
    TTCGACTATC TCATGGCTGA GGAGAAAATT GTGATCTCTA AGAAAGAGTC TTTGTTCTCC 
    TGGGACGAGA CAGAGTACAA GATCCCTGCC CGGCTCACCA TGCAAGTGTG GGACGCTGAC 
    CACTTCTCTG CTGATGACTT CTTGGGTGCG ATTGAGCTGG ACTTGAACCG GTTTCCTCGG 
    GGAGCAAAAA CAGCAAAGCA GTGCTCCATC GACATGGTGC TTAATGAGCA AGAGCTGCCA 
    ACCATCTCAA TCTTCAAACA GAAAAGGGTG AAGGGCTGGT GGCCTTTTGT GGCGCGAGAT 
    GAGAATGATG ACCTGGAGCT GACGGGAAAA GTGGAGGCAG AGCTTCATCT AATGACAGCG 
    GAGGAAGCAG AGAAGAGTCC TGTGGGCCTT GGAAGGAATG AACCAGACCC ACTGGAGAAA 
    CCAAACCGGC CCGACACCAC CTTCCTCTGG TTCCTGAGTC CACTGAAGGC CATCCGCTAC 
    CTGGTGTGCA ACCGCTACAA ATGGCTCATA ATCAAGATTG TCCTGGCCCT GCTGCTGCTG 
    ATCATGGTGG GCCTCTTCCT CTACAGCATG CCTGGCTACC TGGTCAAGAA GCTGCTGGGA 
    GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022541962.1
    CDS267..6332
    Translation

    Target ORF information:

    RefSeq Version XM_022686241.1
    Organism Astyanax mexicanus(Mexican tetra)
    Definition Astyanax mexicanus otoferlin (otof), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_022686241.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TGGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGATGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTGTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCTGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCTTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA ACTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATTGGTCC AGCTGGACGG ACAGAGCTTC CTCCCATTGA TGGGCCAACT 
    GATGTTGACC GAGGTCCCAT CTTACCTGTG CCTCTGGGCA TACGGCCCGT CCTCAGCAGA 
    TACCGCATAG AGGTCTTGTT CTGGGGGCTG CGGGACCTGA AGAGAATTAA CCTGGCGCAG 
    GTCGACAGGC CTCGAGTGGA CATTGAGTGT GCAGGCAAAG GAATCCAATC AGCTCTCAAT 
    CAGAATTACA AGAAAAACCC AAATTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGATCTG 
    CCAGAGAACG AGTTGCTTCA TCCTCCTCTG AATATTCGGG TGGTAGACTG CAGAGCATTT 
    GGCCGCTACA CATTGGTGGG CTCACATGCT GTAACCAGTC TGCGCAAGTT CATCTACATC 
    CCTCCTGACA AAAGGTCCAA TAACTGGGCT AATACAGCTA GACTGGCCAA TGGCTACTTG 
    GCTCTCACCA ATGGGACCTC CCGTTCCCGC CCCTACTCCC GCCCCATCTC TCACTCCTCA 
    GGTGACATCG TGGTCAACAT GGACTCTGAG CCCAATGTCA AGAAGATGGA GACTGTTGTA 
    AAACTGGATG CTACCTCTGA TGCTGTTGTT AAAATTGATG TGAATGAGGA AGAAAAGGAA 
    AAAGACAAAA AGAAGAAAAA GAAAAAGAAG GGAGAGGAGA TAGAAGATGA GGAGCCAGAT 
    GAGAGCATGC TGGACTGGTG GTCAAAGTAC TTTGCTTCAA TAGAGACCAT GATGGAGAAC 
    CTACGAGTCC AGGAAGCAGC ATGGGCTGAG GCTGAGGAGA GAGAAGATTT GGAGATTGCG 
    GCAGAGGGAG CAGAAATCAA AAGTGATGAC TCTTGTACAA AAGGCACGAA GCCCAAAGAG 
    AAGAACAAGA TAAAGAGCTC AAAGGACAAG AAGAAGAGCC ACGGCAGTGA TGGGACAGAG 
    AAAAAAGTGC CCAAACCCAA AGTAGATGAG CTTTTGGTCT ACAATAAGGA GCTGGAGACT 
    GAGTTTGATC ACTTTGAAGA CTGGCTTCAC ACCTTCAACC TGTACAGAGG GAAGGCCATA 
    GATGATGACG ACCAAAACAT TGTGGATGAC GACAGGATTG TGGGCCGATT TAAAGGGTCA 
    TTGTGCATGT ACAAGCTGCC TCTGTCAGAT GAAATCACCA GAGATGTTGG ATTCGATCCA 
    AATATGGGCA TGTTTCAGAG TATCCCACAC AATGATCCCA TCAATGTACT TGTGAGGGTT 
    TATGTTATCA GGGCAACAGA CCTTCACCCT GCAGATATAA ACGGAAAAGC TGATCCATAT 
    GTTGTTATCA AGCTGGGAAA GTCAGAGATC AAGGACAAGG AGAACTACAT CTCTAAACAG 
    CTGAATCCTG TCTTTGGGAA GTCCTTTGAC ATTGAAGCCA CGTTTCCAAT GGAGTCTATG 
    CTGACAGTCT CAGTGTACGA CTGGGATTTA GTTGGCACTG ATGATTTGAT CGGAGAGACC 
    AAGATTGACT TGGAGAACCG TTACTACAGC AAACACAGAG CCACCTGTGG CATCGCATCC 
    AATTACTCTC TTCATGGGTA CAATGTGTGG CGAGACCCTC AGAAGCCCAC CCAGATACTT 
    GCAAAGTTGT GTAAGGAGGG CAAACTGGAT GGACCGCATT ATGGCCCAGG TGGAAAGGTT 
    AAAGTTGCAA ACCGCATCTT TCTCGGGCCA ACGGATATTG AAGATGAAAA TGGTCTAAAG 
    AAGCAAACAG AAGAGCATTT GGCTCTTACT GCACTGAAAC ACTGGGAGGA AATTCCTCGA 
    GTGGGCTGCA AACTCATCCC AGAACATGTA GAAACCCGAC CACTCCTCAA CCCAGATAAA 
    CCTGGAATAG AGCAGGGTCG GCTTGAGATG TGGGTGGATA TGTTCCCAAT GGATGTTCCT 
    TCACCAGGAC CAGCCATAGA CATATCACCA CGGAAACCAA AGAGATATGA GCTCAGGGTG 
    ATAATATGGA ATACTGACGA GGTAATTCTG GAAGACGATG ATTACTTCAC AGGGGAAAAG 
    TCCAGTGACA TTTTTGTCAG GGGCTGGCTG AAAGGACAGC AGGAAGATAA GCAGGACACA 
    GACGTTCACT ACCACTCTCT GACAGGAGAG GGAAACTTCA ACTGGCGCTT TGTGTTTCCC 
    TTCGACTATC TCATGGCTGA GGAGAAAATT GTGATCTCTA AGAAAGAGTC TTTGTTCTCC 
    TGGGACGAGA CAGAGTACAA GATCCCTGCC CGGCTCACCA TGCAAGTGTG GGACGCTGAC 
    CACTTCTCTG CTGATGACTT CTTGGGTGCG ATTGAGCTGG ACTTGAACCG GTTTCCTCGG 
    GGAGCAAAAA CAGCAAAGCA GTGCTCCATC GACATGGTGC TTAATGAGCA AGAGCTGCCA 
    ACCATCTCAA TCTTCAAACA GAAAAGGGTG AAGGGCTGGT GGCCTTTTGT GGCGCGAGAT 
    GAGAATGATG ACCTGGAGCT GACGGGAAAA GTGGAGGCAG AGCTTCATCT AATGACAGCG 
    GAGGAAGCAG AGAAGAGTCC TGTGGGCCTT GGAAGGAATG AACCAGACCC ACTGGAGAAA 
    CCAAACCGGC CCGACACCAC CTTCCTCTGG TTCCTGAGTC CACTGAAGGC CATCCGCTAC 
    CTGGTGTGCA ACCGCTACAA ATGGCTCATA ATCAAGATTG TCCTGGCCCT GCTGCTGCTG 
    ATCATGGTGG GCCTCTTCCT CTACAGCATG CCTGGCTACC TGGTCAAGAA GCTGCTGGGA 
    GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    otof ( XM_015608738.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_015464224.1 Astyanax mexicanus otoferlin (otof), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OAs42390
    Clone ID Related Accession (Same CDS sequence) XM_015608738.1
    Accession Version XM_015608738.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6144bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1455033600000
    Organism Astyanax mexicanus(Mexican tetra)
    Product otoferlin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749495.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TTGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGACGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTCTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCGGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCCTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA GCTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATCCCTTA CGACGATGAG GAGATCAGAA GGGCTCTTAT TGCTGTCCAT 
    GACTTTGCTG TCCCTCAGAT CAAGATTGGT CCAGCTGGAC GGACAGAGCT TCCTCCCATT 
    GATGGGCCAA CTGATGTTGA CCGAGGTCCC ATCTTACCTG TGCCTCTGGG CATACGGCCC 
    GTCCTCAGCA GATACCGCAT AGAGGTCTTG TTCTGGGGGC TGCGGGACCT GAAGAGAATT 
    AACCTGGCGC AGGTCGACAG GCCTCGAGTG GACATTGAGT GTGCAGGCAA AGGAATCCAA 
    TCAGCTCTCA TTCAGAATTA CAAGAAAAAC CCAAATTTCA GCACCCTGGT CAAGTGGTTT 
    GAAGTGGATC TGCCAGAGAA CGAGTTGCTT CATCCTCCTC TGAATATTCG GGTGGTAGAC 
    TGCAGAGCAT TTGGCCGCTA CACATTGGTG GGCTCACATG CTGTAACCAG TCTGCGCAAG 
    TTCATCTACA TCCCTCCTGA CAAAAGGTCC AATAACTGGG CTAATACAGC TAGACTGGCC 
    AATGGCTACT TGGCTCTCAC CAATGGGACC TCCCGTTCCC GCCCCTACTC CCGCCCCATC 
    TCTCACTCCT CAGGTGACAT CGTGGTCAAC ATGGACTCTG AGCCCAATGT CAAGAAGATG 
    GAGACTGTTG TAAAACTGGA TGCTACCTCT GATGCTGTTG TTAAAATTGA TGTGAATGAG 
    GAAGAAAAGG AAAAAGACAA AAAGAAGAAA AAGAAAAAGA AGGGAGAGGA GATAGAAGAT 
    GAGGAGCCAG ATGAGAGCAT GCTGGACTGG TGGTCAAAGT ACTTTGCTTC AATAGAGACC 
    ATGATGGAGA ACCTACGAGT CCAGGAAGCA GCATGGGCTG AGGCTGAGGA GAGAGAAGAT 
    TTGGAGATTG CGGCAGAGGG AGCAGAAATC AAAAGTGATG ACTCTTGTAC AAAAGGCACG 
    AAGCCCAAAG AGAAGAACAA GATAAAGAGC TCAAAGGACA AGAAGAAGAG CCACGGCAGT 
    GATGGGACAG AGAAAAAAGT GCCCAAACCC AAAGTAGACG AGCTTTTGGT CTACAATAAG 
    GAGCTGGAGA CTGAGTTTGA TCACTTTGAA GACTGGCTTC ACACCTTCAA CCTGTACAGA 
    GGGAAGGCCA TAGATGATGA TGACCAAAAC ATTGTGGATG ACGACAGGAT TGTGGGCCGA 
    TTTAAAGGGT CATTGTGCAT GTACAAGCTG CCTCTGTCAG ATGAAATCAC CAGAGATGTT 
    GGATTCGATC CAAATATGGG CATGTTTCAG AGTATCCCAC ACAATGATCC CATCAATGTA 
    CTTGTGAGGG TTTATGTTAT CAGGGCAACA GACCTTCACC CTGCAGATAT AAACGGAAAA 
    GCTGATCCAT ATGTTGTTAT CAAGCTGGGA AAGTCAGAGA TCAAGGACAA GGAGAACTAC 
    ATCTCTAAAC AGCTGAATCC TGTCTTTGGG AAGTCCTTTG ACATTGAAGC CACGTTTCCA 
    ATGGAGTCTA TGCTGACAGT CTCAGTGTAC GACTGGGATT TAGTTGGCAC TGATGATTTG 
    ATCGGAGAGA CCAAGATTGA CTTGGAGAAC CGTTACTACA GCAAACACAG AGCCACCTGT 
    GGCATCGCAT CCAATTACTC TCTTCATGGG TACAATGTGT GGCGAGACCC TCAGAAGCCC 
    ACCCAGATAC TTGCAAAGTT GTGTAAGGAG GGCAAACTGG ATGGACCGCA TTATGGCCCA 
    GGTGGAAAGG TTAAAGTTGC AAACCGCATC TTTCTCGGGC CAACGGATAT TGAAGATGAA 
    AATGGTCTAA AGAAGCAAAC AGAAGAGCAT TTGGCTCTTA CTGCACTGAA ACACTGGGAG 
    GAAATTCCTC GAGTGGGCTG CAAACTCATC CCAGAACATG TAGAAACCCG ACCACTCCTC 
    AACCCAGATA AACCTGGAAT AGAGCAGGGT CGGCTTGAGA TGTGGGTGGA TATGTTCCCA 
    ATGGATGTTC CTTCACCAGG ACCAGCCATA GACATATCAC CACGGAAACC AAAGAGATAT 
    GAGCTCAGGG TGATAATATG GAATACTGAC GAGGTAATTC TGGAAGACGA TGATTACTTC 
    ACAGGGGAAA AGTCCAGTGA CATTTTTGTC AGGGGCTGGC TGAAAGGACA GCAGGAAGAT 
    AAGCAGGACA CAGACGTTCA CTACCACTCT CTGACAGGAG AGGGAAACTT CAACTGGCGC 
    TTTGTGTTTC CCTTCGACTA TCTCATGGCT GAGGAGAAAA TTGTGATCTC TAAGAAAGAG 
    TCTTTGTTCT CCTGGGACGA GACAGAGTAC AAGATCCCTG CCCGGCTCAC CATGCAAGTG 
    TGGGACGCTG ACCACTTCTC TGCTGATGAC TTCTTGGGTG CGATTGAGCT GGACTTGAAC 
    CGGTTTCCTC GGGGAGCAAA AACAGCAAAG CAGTGCTCCA TCGACATGGT GCTTAATGAG 
    CAAGAGCTGC CAACCATCTC AATCTTCAAA CAGAAAAGGG TGAAGGGCTG GTGGCCTTTT 
    GTGGCGCGAG ATGAGAATGA TGACCTGGAG CTGACGGGAA AAGTGGAGGC AGAGCTTCAT 
    CTAATGACAG CAGAGGAAGC AGAGAAGAGT CCTGTGGGCC TTGGAAGGAA TGAACCAGAC 
    CCACTGGAGA AACCAAACCG TCCCAACACC AGCTTGTCGT GGTTGTTGAG TCCGATGCGA 
    GTGGCGTGTA TCCTGGCATG GAGGCAGTAT AAGATGCCGT GTTTGGTGCT GCTTCTCTGT 
    ACAGCAGGCC TGATATTCCT GGGCCTGCTC ATCTTCTCCA TTCCTTCTGC TCTCAGTCAG 
    AAGATGGTCA GTTTTCTAGG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015464224.1
    CDS134..6277
    Translation

    Target ORF information:

    RefSeq Version XM_015608738.1
    Organism Astyanax mexicanus(Mexican tetra)
    Definition Astyanax mexicanus otoferlin (otof), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_015608738.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TTGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGACGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTCTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCGGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCCTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA GCTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATCCCTTA CGACGATGAG GAGATCAGAA GGGCTCTTAT TGCTGTCCAT 
    GACTTTGCTG TCCCTCAGAT CAAGATTGGT CCAGCTGGAC GGACAGAGCT TCCTCCCATT 
    GATGGGCCAA CTGATGTTGA CCGAGGTCCC ATCTTACCTG TGCCTCTGGG CATACGGCCC 
    GTCCTCAGCA GATACCGCAT AGAGGTCTTG TTCTGGGGGC TGCGGGACCT GAAGAGAATT 
    AACCTGGCGC AGGTCGACAG GCCTCGAGTG GACATTGAGT GTGCAGGCAA AGGAATCCAA 
    TCAGCTCTCA TTCAGAATTA CAAGAAAAAC CCAAATTTCA GCACCCTGGT CAAGTGGTTT 
    GAAGTGGATC TGCCAGAGAA CGAGTTGCTT CATCCTCCTC TGAATATTCG GGTGGTAGAC 
    TGCAGAGCAT TTGGCCGCTA CACATTGGTG GGCTCACATG CTGTAACCAG TCTGCGCAAG 
    TTCATCTACA TCCCTCCTGA CAAAAGGTCC AATAACTGGG CTAATACAGC TAGACTGGCC 
    AATGGCTACT TGGCTCTCAC CAATGGGACC TCCCGTTCCC GCCCCTACTC CCGCCCCATC 
    TCTCACTCCT CAGGTGACAT CGTGGTCAAC ATGGACTCTG AGCCCAATGT CAAGAAGATG 
    GAGACTGTTG TAAAACTGGA TGCTACCTCT GATGCTGTTG TTAAAATTGA TGTGAATGAG 
    GAAGAAAAGG AAAAAGACAA AAAGAAGAAA AAGAAAAAGA AGGGAGAGGA GATAGAAGAT 
    GAGGAGCCAG ATGAGAGCAT GCTGGACTGG TGGTCAAAGT ACTTTGCTTC AATAGAGACC 
    ATGATGGAGA ACCTACGAGT CCAGGAAGCA GCATGGGCTG AGGCTGAGGA GAGAGAAGAT 
    TTGGAGATTG CGGCAGAGGG AGCAGAAATC AAAAGTGATG ACTCTTGTAC AAAAGGCACG 
    AAGCCCAAAG AGAAGAACAA GATAAAGAGC TCAAAGGACA AGAAGAAGAG CCACGGCAGT 
    GATGGGACAG AGAAAAAAGT GCCCAAACCC AAAGTAGACG AGCTTTTGGT CTACAATAAG 
    GAGCTGGAGA CTGAGTTTGA TCACTTTGAA GACTGGCTTC ACACCTTCAA CCTGTACAGA 
    GGGAAGGCCA TAGATGATGA TGACCAAAAC ATTGTGGATG ACGACAGGAT TGTGGGCCGA 
    TTTAAAGGGT CATTGTGCAT GTACAAGCTG CCTCTGTCAG ATGAAATCAC CAGAGATGTT 
    GGATTCGATC CAAATATGGG CATGTTTCAG AGTATCCCAC ACAATGATCC CATCAATGTA 
    CTTGTGAGGG TTTATGTTAT CAGGGCAACA GACCTTCACC CTGCAGATAT AAACGGAAAA 
    GCTGATCCAT ATGTTGTTAT CAAGCTGGGA AAGTCAGAGA TCAAGGACAA GGAGAACTAC 
    ATCTCTAAAC AGCTGAATCC TGTCTTTGGG AAGTCCTTTG ACATTGAAGC CACGTTTCCA 
    ATGGAGTCTA TGCTGACAGT CTCAGTGTAC GACTGGGATT TAGTTGGCAC TGATGATTTG 
    ATCGGAGAGA CCAAGATTGA CTTGGAGAAC CGTTACTACA GCAAACACAG AGCCACCTGT 
    GGCATCGCAT CCAATTACTC TCTTCATGGG TACAATGTGT GGCGAGACCC TCAGAAGCCC 
    ACCCAGATAC TTGCAAAGTT GTGTAAGGAG GGCAAACTGG ATGGACCGCA TTATGGCCCA 
    GGTGGAAAGG TTAAAGTTGC AAACCGCATC TTTCTCGGGC CAACGGATAT TGAAGATGAA 
    AATGGTCTAA AGAAGCAAAC AGAAGAGCAT TTGGCTCTTA CTGCACTGAA ACACTGGGAG 
    GAAATTCCTC GAGTGGGCTG CAAACTCATC CCAGAACATG TAGAAACCCG ACCACTCCTC 
    AACCCAGATA AACCTGGAAT AGAGCAGGGT CGGCTTGAGA TGTGGGTGGA TATGTTCCCA 
    ATGGATGTTC CTTCACCAGG ACCAGCCATA GACATATCAC CACGGAAACC AAAGAGATAT 
    GAGCTCAGGG TGATAATATG GAATACTGAC GAGGTAATTC TGGAAGACGA TGATTACTTC 
    ACAGGGGAAA AGTCCAGTGA CATTTTTGTC AGGGGCTGGC TGAAAGGACA GCAGGAAGAT 
    AAGCAGGACA CAGACGTTCA CTACCACTCT CTGACAGGAG AGGGAAACTT CAACTGGCGC 
    TTTGTGTTTC CCTTCGACTA TCTCATGGCT GAGGAGAAAA TTGTGATCTC TAAGAAAGAG 
    TCTTTGTTCT CCTGGGACGA GACAGAGTAC AAGATCCCTG CCCGGCTCAC CATGCAAGTG 
    TGGGACGCTG ACCACTTCTC TGCTGATGAC TTCTTGGGTG CGATTGAGCT GGACTTGAAC 
    CGGTTTCCTC GGGGAGCAAA AACAGCAAAG CAGTGCTCCA TCGACATGGT GCTTAATGAG 
    CAAGAGCTGC CAACCATCTC AATCTTCAAA CAGAAAAGGG TGAAGGGCTG GTGGCCTTTT 
    GTGGCGCGAG ATGAGAATGA TGACCTGGAG CTGACGGGAA AAGTGGAGGC AGAGCTTCAT 
    CTAATGACAG CAGAGGAAGC AGAGAAGAGT CCTGTGGGCC TTGGAAGGAA TGAACCAGAC 
    CCACTGGAGA AACCAAACCG TCCCAACACC AGCTTGTCGT GGTTGTTGAG TCCGATGCGA 
    GTGGCGTGTA TCCTGGCATG GAGGCAGTAT AAGATGCCGT GTTTGGTGCT GCTTCTCTGT 
    ACAGCAGGCC TGATATTCCT GGGCCTGCTC ATCTTCTCCA TTCCTTCTGC TCTCAGTCAG 
    AAGATGGTCA GTTTTCTAGG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    otof ( XM_007260866.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007260928.1 Astyanax mexicanus otoferlin (otof), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OAs42391
    Clone ID Related Accession (Same CDS sequence) XM_007260866.2
    Accession Version XM_007260866.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1455033600000
    Organism Astyanax mexicanus(Mexican tetra)
    Product otoferlin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749495.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007260866.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TTGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGACGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTCTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCGGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCCTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA GCTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATCCCTTA CGACGATGAG GAGATCAGAA GGGCTCTTAT TGCTGTCCAT 
    GACTTTGCTG TCCCTCAGAT CAAGATTGGT CCAGCTGGAC GGACAGAGCT TCCTCCCATT 
    GATGGGCCAA CTGATGTTGA CCGAGGTCCC ATCTTACCTG TGCCTCTGGG CATACGGCCC 
    GTCCTCAGCA GATACCGCAT AGAGGTCTTG TTCTGGGGGC TGCGGGACCT GAAGAGAATT 
    AACCTGGCGC AGGTCGACAG GCCTCGAGTG GACATTGAGT GTGCAGGCAA AGGAATCCAA 
    TCAGCTCTCA TTCAGAATTA CAAGAAAAAC CCAAATTTCA GCACCCTGGT CAAGTGGTTT 
    GAAGTGGATC TGCCAGAGAA CGAGTTGCTT CATCCTCCTC TGAATATTCG GGTGGTAGAC 
    TGCAGAGCAT TTGGCCGCTA CACATTGGTG GGCTCACATG CTGTAACCAG TCTGCGCAAG 
    TTCATCTACA TCCCTCCTGA CAAAAGGTCC AATAACTGGG CTAATACAGC TAGACTGGCC 
    AATGGCTACT TGGCTCTCAC CAATGGGACC TCCCGTTCCC GCCCCTACTC CCGCCCCATC 
    TCTCACTCCT CAGGTGACAT CGTGGTCAAC ATGGACTCTG AGCCCAATGT CAAGAAGATG 
    GAGACTGTTG TAAAACTGGA TGCTACCTCT GATGCTGTTG TTAAAATTGA TGTGAATGAG 
    GAAGAAAAGG AAAAAGACAA AAAGAAGAAA AAGAAAAAGA AGGGAGAGGA GATAGAAGAT 
    GAGGAGCCAG ATGAGAGCAT GCTGGACTGG TGGTCAAAGT ACTTTGCTTC AATAGAGACC 
    ATGATGGAGA ACCTACGAGT CCAGGAAGCA GCATGGGCTG AGGCTGAGGA GAGAGAAGAT 
    TTGGAGATTG CGGCAGAGGG AGCAGAAATC AAAAGTGATG ACTCTTGTAC AAAAGGCACG 
    AAGCCCAAAG AGAAGAACAA GATAAAGAGC TCAAAGGACA AGAAGAAGAG CCACGGCAGT 
    GATGGGACAG AGAAAAAAGT GCCCAAACCC AAAGTAGACG AGCTTTTGGT CTACAATAAG 
    GAGCTGGAGA CTGAGTTTGA TCACTTTGAA GACTGGCTTC ACACCTTCAA CCTGTACAGA 
    GGGAAGGCCA TAGATGATGA TGACCAAAAC ATTGTGGATG ACGACAGGAT TGTGGGCCGA 
    TTTAAAGGGT CATTGTGCAT GTACAAGCTG CCTCTGTCAG ATGAAATCAC CAGAGATGTT 
    GGATTCGATC CAAATATGGG CATGTTTCAG AGTATCCCAC ACAATGATCC CATCAATGTA 
    CTTGTGAGGG TTTATGTTAT CAGGGCAACA GACCTTCACC CTGCAGATAT AAACGGAAAA 
    GCTGATCCAT ATGTTGTTAT CAAGCTGGGA AAGTCAGAGA TCAAGGACAA GGAGAACTAC 
    ATCTCTAAAC AGCTGAATCC TGTCTTTGGG AAGTCCTTTG ACATTGAAGC CACGTTTCCA 
    ATGGAGTCTA TGCTGACAGT CTCAGTGTAC GACTGGGATT TAGTTGGCAC TGATGATTTG 
    ATCGGAGAGA CCAAGATTGA CTTGGAGAAC CGTTACTACA GCAAACACAG AGCCACCTGT 
    GGCATCGCAT CCAATTACTC TCTTCATGGG TACAATGTGT GGCGAGACCC TCAGAAGCCC 
    ACCCAGATAC TTGCAAAGTT GTGTAAGGAG GGCAAACTGG ATGGACCGCA TTATGGCCCA 
    GGTGGAAAGG TTAAAGTTGC AAACCGCATC TTTCTCGGGC CAACGGATAT TGAAGATGAA 
    AATGGTCTAA AGAAGCAAAC AGAAGAGCAT TTGGCTCTTA CTGCACTGAA ACACTGGGAG 
    GAAATTCCTC GAGTGGGCTG CAAACTCATC CCAGAACATG TAGAAACCCG ACCACTCCTC 
    AACCCAGATA AACCTGGAAT AGAGCAGGGT CGGCTTGAGA TGTGGGTGGA TATGTTCCCA 
    ATGGATGTTC CTTCACCAGG ACCAGCCATA GACATATCAC CACGGAAACC AAAGAGATAT 
    GAGCTCAGGG TGATAATATG GAATACTGAC GAGGTAATTC TGGAAGACGA TGATTACTTC 
    ACAGGGGAAA AGTCCAGTGA CATTTTTGTC AGGGGCTGGC TGAAAGGACA GCAGGAAGAT 
    AAGCAGGACA CAGACGTTCA CTACCACTCT CTGACAGGAG AGGGAAACTT CAACTGGCGC 
    TTTGTGTTTC CCTTCGACTA TCTCATGGCT GAGGAGAAAA TTGTGATCTC TAAGAAAGAG 
    TCTTTGTTCT CCTGGGACGA GACAGAGTAC AAGATCCCTG CCCGGCTCAC CATGCAAGTG 
    TGGGACGCTG ACCACTTCTC TGCTGATGAC TTCTTGGGTG CGATTGAGCT GGACTTGAAC 
    CGGTTTCCTC GGGGAGCAAA AACAGCAAAG CAGTGCTCCA TCGACATGGT GCTTAATGAG 
    CAAGAGCTGC CAACCATCTC AATCTTCAAA CAGAAAAGGG TGAAGGGCTG GTGGCCTTTT 
    GTGGCGCGAG ATGAGAATGA TGACCTGGAG CTGACGGGAA AAGTGGAGGC AGAGCTTCAT 
    CTAATGACAG CAGAGGAAGC AGAGAAGAGT CCTGTGGGCC TTGGAAGGAA TGAACCAGAC 
    CCACTGGAGA AACCAAATCG CCCGGACACC AGTCTCATGT GGTTCATGAA CCCCCTGAAG 
    TCCCTGCGAT ACTTCATTTG GCACAACTAC CGCTGGCTGA TCCTGAAGAC CCTAGGGCTG 
    ATGCTGCTCC TCCTGCTCCT CGGCCTCTTT CTCTACTCCA TCCCTGGATA CCTGGTCAAG 
    AAGCTGCTGG GGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_007260928.1
    CDS134..6271
    Translation

    Target ORF information:

    RefSeq Version XM_007260866.2
    Organism Astyanax mexicanus(Mexican tetra)
    Definition Astyanax mexicanus otoferlin (otof), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_007260866.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TTGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGACGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTCTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCGGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCCTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA GCTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATCCCTTA CGACGATGAG GAGATCAGAA GGGCTCTTAT TGCTGTCCAT 
    GACTTTGCTG TCCCTCAGAT CAAGATTGGT CCAGCTGGAC GGACAGAGCT TCCTCCCATT 
    GATGGGCCAA CTGATGTTGA CCGAGGTCCC ATCTTACCTG TGCCTCTGGG CATACGGCCC 
    GTCCTCAGCA GATACCGCAT AGAGGTCTTG TTCTGGGGGC TGCGGGACCT GAAGAGAATT 
    AACCTGGCGC AGGTCGACAG GCCTCGAGTG GACATTGAGT GTGCAGGCAA AGGAATCCAA 
    TCAGCTCTCA TTCAGAATTA CAAGAAAAAC CCAAATTTCA GCACCCTGGT CAAGTGGTTT 
    GAAGTGGATC TGCCAGAGAA CGAGTTGCTT CATCCTCCTC TGAATATTCG GGTGGTAGAC 
    TGCAGAGCAT TTGGCCGCTA CACATTGGTG GGCTCACATG CTGTAACCAG TCTGCGCAAG 
    TTCATCTACA TCCCTCCTGA CAAAAGGTCC AATAACTGGG CTAATACAGC TAGACTGGCC 
    AATGGCTACT TGGCTCTCAC CAATGGGACC TCCCGTTCCC GCCCCTACTC CCGCCCCATC 
    TCTCACTCCT CAGGTGACAT CGTGGTCAAC ATGGACTCTG AGCCCAATGT CAAGAAGATG 
    GAGACTGTTG TAAAACTGGA TGCTACCTCT GATGCTGTTG TTAAAATTGA TGTGAATGAG 
    GAAGAAAAGG AAAAAGACAA AAAGAAGAAA AAGAAAAAGA AGGGAGAGGA GATAGAAGAT 
    GAGGAGCCAG ATGAGAGCAT GCTGGACTGG TGGTCAAAGT ACTTTGCTTC AATAGAGACC 
    ATGATGGAGA ACCTACGAGT CCAGGAAGCA GCATGGGCTG AGGCTGAGGA GAGAGAAGAT 
    TTGGAGATTG CGGCAGAGGG AGCAGAAATC AAAAGTGATG ACTCTTGTAC AAAAGGCACG 
    AAGCCCAAAG AGAAGAACAA GATAAAGAGC TCAAAGGACA AGAAGAAGAG CCACGGCAGT 
    GATGGGACAG AGAAAAAAGT GCCCAAACCC AAAGTAGACG AGCTTTTGGT CTACAATAAG 
    GAGCTGGAGA CTGAGTTTGA TCACTTTGAA GACTGGCTTC ACACCTTCAA CCTGTACAGA 
    GGGAAGGCCA TAGATGATGA TGACCAAAAC ATTGTGGATG ACGACAGGAT TGTGGGCCGA 
    TTTAAAGGGT CATTGTGCAT GTACAAGCTG CCTCTGTCAG ATGAAATCAC CAGAGATGTT 
    GGATTCGATC CAAATATGGG CATGTTTCAG AGTATCCCAC ACAATGATCC CATCAATGTA 
    CTTGTGAGGG TTTATGTTAT CAGGGCAACA GACCTTCACC CTGCAGATAT AAACGGAAAA 
    GCTGATCCAT ATGTTGTTAT CAAGCTGGGA AAGTCAGAGA TCAAGGACAA GGAGAACTAC 
    ATCTCTAAAC AGCTGAATCC TGTCTTTGGG AAGTCCTTTG ACATTGAAGC CACGTTTCCA 
    ATGGAGTCTA TGCTGACAGT CTCAGTGTAC GACTGGGATT TAGTTGGCAC TGATGATTTG 
    ATCGGAGAGA CCAAGATTGA CTTGGAGAAC CGTTACTACA GCAAACACAG AGCCACCTGT 
    GGCATCGCAT CCAATTACTC TCTTCATGGG TACAATGTGT GGCGAGACCC TCAGAAGCCC 
    ACCCAGATAC TTGCAAAGTT GTGTAAGGAG GGCAAACTGG ATGGACCGCA TTATGGCCCA 
    GGTGGAAAGG TTAAAGTTGC AAACCGCATC TTTCTCGGGC CAACGGATAT TGAAGATGAA 
    AATGGTCTAA AGAAGCAAAC AGAAGAGCAT TTGGCTCTTA CTGCACTGAA ACACTGGGAG 
    GAAATTCCTC GAGTGGGCTG CAAACTCATC CCAGAACATG TAGAAACCCG ACCACTCCTC 
    AACCCAGATA AACCTGGAAT AGAGCAGGGT CGGCTTGAGA TGTGGGTGGA TATGTTCCCA 
    ATGGATGTTC CTTCACCAGG ACCAGCCATA GACATATCAC CACGGAAACC AAAGAGATAT 
    GAGCTCAGGG TGATAATATG GAATACTGAC GAGGTAATTC TGGAAGACGA TGATTACTTC 
    ACAGGGGAAA AGTCCAGTGA CATTTTTGTC AGGGGCTGGC TGAAAGGACA GCAGGAAGAT 
    AAGCAGGACA CAGACGTTCA CTACCACTCT CTGACAGGAG AGGGAAACTT CAACTGGCGC 
    TTTGTGTTTC CCTTCGACTA TCTCATGGCT GAGGAGAAAA TTGTGATCTC TAAGAAAGAG 
    TCTTTGTTCT CCTGGGACGA GACAGAGTAC AAGATCCCTG CCCGGCTCAC CATGCAAGTG 
    TGGGACGCTG ACCACTTCTC TGCTGATGAC TTCTTGGGTG CGATTGAGCT GGACTTGAAC 
    CGGTTTCCTC GGGGAGCAAA AACAGCAAAG CAGTGCTCCA TCGACATGGT GCTTAATGAG 
    CAAGAGCTGC CAACCATCTC AATCTTCAAA CAGAAAAGGG TGAAGGGCTG GTGGCCTTTT 
    GTGGCGCGAG ATGAGAATGA TGACCTGGAG CTGACGGGAA AAGTGGAGGC AGAGCTTCAT 
    CTAATGACAG CAGAGGAAGC AGAGAAGAGT CCTGTGGGCC TTGGAAGGAA TGAACCAGAC 
    CCACTGGAGA AACCAAATCG CCCGGACACC AGTCTCATGT GGTTCATGAA CCCCCTGAAG 
    TCCCTGCGAT ACTTCATTTG GCACAACTAC CGCTGGCTGA TCCTGAAGAC CCTAGGGCTG 
    ATGCTGCTCC TCCTGCTCCT CGGCCTCTTT CTCTACTCCA TCCCTGGATA CCTGGTCAAG 
    AAGCTGCTGG GGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    otof ( XM_007260865.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007260927.1 Astyanax mexicanus otoferlin (otof), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OAs42392
    Clone ID Related Accession (Same CDS sequence) XM_007260865.2
    Accession Version XM_007260865.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1455033600000
    Organism Astyanax mexicanus(Mexican tetra)
    Product otoferlin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749495.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007260865.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TTGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGACGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTCTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCGGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCCTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA GCTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATCCCTTA CGACGATGAG GAGATCAGAA GGGCTCTTAT TGCTGTCCAT 
    GACTTTGCTG TCCCTCAGAT CAAGATTGGT CCAGCTGGAC GGACAGAGCT TCCTCCCATT 
    GATGGGCCAA CTGATGTTGA CCGAGGTCCC ATCTTACCTG TGCCTCTGGG CATACGGCCC 
    GTCCTCAGCA GATACCGCAT AGAGGTCTTG TTCTGGGGGC TGCGGGACCT GAAGAGAATT 
    AACCTGGCGC AGGTCGACAG GCCTCGAGTG GACATTGAGT GTGCAGGCAA AGGAATCCAA 
    TCAGCTCTCA TTCAGAATTA CAAGAAAAAC CCAAATTTCA GCACCCTGGT CAAGTGGTTT 
    GAAGTGGATC TGCCAGAGAA CGAGTTGCTT CATCCTCCTC TGAATATTCG GGTGGTAGAC 
    TGCAGAGCAT TTGGCCGCTA CACATTGGTG GGCTCACATG CTGTAACCAG TCTGCGCAAG 
    TTCATCTACA TCCCTCCTGA CAAAAGGTCC AATAACTGGG CTAATACAGC TAGACTGGCC 
    AATGGCTACT TGGCTCTCAC CAATGGGACC TCCCGTTCCC GCCCCTACTC CCGCCCCATC 
    TCTCACTCCT CAGGTGACAT CGTGGTCAAC ATGGACTCTG AGCCCAATGT CAAGAAGATG 
    GAGACTGTTG TAAAACTGGA TGCTACCTCT GATGCTGTTG TTAAAATTGA TGTGAATGAG 
    GAAGAAAAGG AAAAAGACAA AAAGAAGAAA AAGAAAAAGA AGGGAGAGGA GATAGAAGAT 
    GAGGAGCCAG ATGAGAGCAT GCTGGACTGG TGGTCAAAGT ACTTTGCTTC AATAGAGACC 
    ATGATGGAGA ACCTACGAGT CCAGGAAGCA GCATGGGCTG AGGCTGAGGA GAGAGAAGAT 
    TTGGAGATTG CGGCAGAGGG AGCAGAAATC AAAAGTGATG ACTCTTGTAC AAAAGGCACG 
    AAGCCCAAAG AGAAGAACAA GATAAAGAGC TCAAAGGACA AGAAGAAGAG CCACGGCAGT 
    GATGGGACAG AGAAAAAAGT GCCCAAACCC AAAGTAGACG AGCTTTTGGT CTACAATAAG 
    GAGCTGGAGA CTGAGTTTGA TCACTTTGAA GACTGGCTTC ACACCTTCAA CCTGTACAGA 
    GGGAAGGCCA TAGATGATGA TGACCAAAAC ATTGTGGATG ACGACAGGAT TGTGGGCCGA 
    TTTAAAGGGT CATTGTGCAT GTACAAGCTG CCTCTGTCAG ATGAAATCAC CAGAGATGTT 
    GGATTCGATC CAAATATGGG CATGTTTCAG AGTATCCCAC ACAATGATCC CATCAATGTA 
    CTTGTGAGGG TTTATGTTAT CAGGGCAACA GACCTTCACC CTGCAGATAT AAACGGAAAA 
    GCTGATCCAT ATGTTGTTAT CAAGCTGGGA AAGTCAGAGA TCAAGGACAA GGAGAACTAC 
    ATCTCTAAAC AGCTGAATCC TGTCTTTGGG AAGTCCTTTG ACATTGAAGC CACGTTTCCA 
    ATGGAGTCTA TGCTGACAGT CTCAGTGTAC GACTGGGATT TAGTTGGCAC TGATGATTTG 
    ATCGGAGAGA CCAAGATTGA CTTGGAGAAC CGTTACTACA GCAAACACAG AGCCACCTGT 
    GGCATCGCAT CCAATTACTC TCTTCATGGG TACAATGTGT GGCGAGACCC TCAGAAGCCC 
    ACCCAGATAC TTGCAAAGTT GTGTAAGGAG GGCAAACTGG ATGGACCGCA TTATGGCCCA 
    GGTGGAAAGG TTAAAGTTGC AAACCGCATC TTTCTCGGGC CAACGGATAT TGAAGATGAA 
    AATGGTCTAA AGAAGCAAAC AGAAGAGCAT TTGGCTCTTA CTGCACTGAA ACACTGGGAG 
    GAAATTCCTC GAGTGGGCTG CAAACTCATC CCAGAACATG TAGAAACCCG ACCACTCCTC 
    AACCCAGATA AACCTGGAAT AGAGCAGGGT CGGCTTGAGA TGTGGGTGGA TATGTTCCCA 
    ATGGATGTTC CTTCACCAGG ACCAGCCATA GACATATCAC CACGGAAACC AAAGAGATAT 
    GAGCTCAGGG TGATAATATG GAATACTGAC GAGGTAATTC TGGAAGACGA TGATTACTTC 
    ACAGGGGAAA AGTCCAGTGA CATTTTTGTC AGGGGCTGGC TGAAAGGACA GCAGGAAGAT 
    AAGCAGGACA CAGACGTTCA CTACCACTCT CTGACAGGAG AGGGAAACTT CAACTGGCGC 
    TTTGTGTTTC CCTTCGACTA TCTCATGGCT GAGGAGAAAA TTGTGATCTC TAAGAAAGAG 
    TCTTTGTTCT CCTGGGACGA GACAGAGTAC AAGATCCCTG CCCGGCTCAC CATGCAAGTG 
    TGGGACGCTG ACCACTTCTC TGCTGATGAC TTCTTGGGTG CGATTGAGCT GGACTTGAAC 
    CGGTTTCCTC GGGGAGCAAA AACAGCAAAG CAGTGCTCCA TCGACATGGT GCTTAATGAG 
    CAAGAGCTGC CAACCATCTC AATCTTCAAA CAGAAAAGGG TGAAGGGCTG GTGGCCTTTT 
    GTGGCGCGAG ATGAGAATGA TGACCTGGAG CTGACGGGAA AAGTGGAGGC AGAGCTTCAT 
    CTAATGACAG CAGAGGAAGC AGAGAAGAGT CCTGTGGGCC TTGGAAGGAA TGAACCAGAC 
    CCACTGGAGA AACCAAACCG GCCCGACACC ACCTTCCTCT GGTTCCTGAG TCCACTGAAG 
    GCCATCCGCT ACCTGGTGTG CAACCGCTAC AAATGGCTCA TAATCAAGAT TGTCCTGGCC 
    CTGCTGCTGC TGATCATGGT GGGCCTCTTC CTCTACAGCA TGCCTGGCTA CCTGGTCAAG 
    AAGCTGCTGG GAGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_007260927.1
    CDS134..6271
    Translation

    Target ORF information:

    RefSeq Version XM_007260865.2
    Organism Astyanax mexicanus(Mexican tetra)
    Definition Astyanax mexicanus otoferlin (otof), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_007260865.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TTGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGACGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTCTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCGGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCCTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA GCTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATCCCTTA CGACGATGAG GAGATCAGAA GGGCTCTTAT TGCTGTCCAT 
    GACTTTGCTG TCCCTCAGAT CAAGATTGGT CCAGCTGGAC GGACAGAGCT TCCTCCCATT 
    GATGGGCCAA CTGATGTTGA CCGAGGTCCC ATCTTACCTG TGCCTCTGGG CATACGGCCC 
    GTCCTCAGCA GATACCGCAT AGAGGTCTTG TTCTGGGGGC TGCGGGACCT GAAGAGAATT 
    AACCTGGCGC AGGTCGACAG GCCTCGAGTG GACATTGAGT GTGCAGGCAA AGGAATCCAA 
    TCAGCTCTCA TTCAGAATTA CAAGAAAAAC CCAAATTTCA GCACCCTGGT CAAGTGGTTT 
    GAAGTGGATC TGCCAGAGAA CGAGTTGCTT CATCCTCCTC TGAATATTCG GGTGGTAGAC 
    TGCAGAGCAT TTGGCCGCTA CACATTGGTG GGCTCACATG CTGTAACCAG TCTGCGCAAG 
    TTCATCTACA TCCCTCCTGA CAAAAGGTCC AATAACTGGG CTAATACAGC TAGACTGGCC 
    AATGGCTACT TGGCTCTCAC CAATGGGACC TCCCGTTCCC GCCCCTACTC CCGCCCCATC 
    TCTCACTCCT CAGGTGACAT CGTGGTCAAC ATGGACTCTG AGCCCAATGT CAAGAAGATG 
    GAGACTGTTG TAAAACTGGA TGCTACCTCT GATGCTGTTG TTAAAATTGA TGTGAATGAG 
    GAAGAAAAGG AAAAAGACAA AAAGAAGAAA AAGAAAAAGA AGGGAGAGGA GATAGAAGAT 
    GAGGAGCCAG ATGAGAGCAT GCTGGACTGG TGGTCAAAGT ACTTTGCTTC AATAGAGACC 
    ATGATGGAGA ACCTACGAGT CCAGGAAGCA GCATGGGCTG AGGCTGAGGA GAGAGAAGAT 
    TTGGAGATTG CGGCAGAGGG AGCAGAAATC AAAAGTGATG ACTCTTGTAC AAAAGGCACG 
    AAGCCCAAAG AGAAGAACAA GATAAAGAGC TCAAAGGACA AGAAGAAGAG CCACGGCAGT 
    GATGGGACAG AGAAAAAAGT GCCCAAACCC AAAGTAGACG AGCTTTTGGT CTACAATAAG 
    GAGCTGGAGA CTGAGTTTGA TCACTTTGAA GACTGGCTTC ACACCTTCAA CCTGTACAGA 
    GGGAAGGCCA TAGATGATGA TGACCAAAAC ATTGTGGATG ACGACAGGAT TGTGGGCCGA 
    TTTAAAGGGT CATTGTGCAT GTACAAGCTG CCTCTGTCAG ATGAAATCAC CAGAGATGTT 
    GGATTCGATC CAAATATGGG CATGTTTCAG AGTATCCCAC ACAATGATCC CATCAATGTA 
    CTTGTGAGGG TTTATGTTAT CAGGGCAACA GACCTTCACC CTGCAGATAT AAACGGAAAA 
    GCTGATCCAT ATGTTGTTAT CAAGCTGGGA AAGTCAGAGA TCAAGGACAA GGAGAACTAC 
    ATCTCTAAAC AGCTGAATCC TGTCTTTGGG AAGTCCTTTG ACATTGAAGC CACGTTTCCA 
    ATGGAGTCTA TGCTGACAGT CTCAGTGTAC GACTGGGATT TAGTTGGCAC TGATGATTTG 
    ATCGGAGAGA CCAAGATTGA CTTGGAGAAC CGTTACTACA GCAAACACAG AGCCACCTGT 
    GGCATCGCAT CCAATTACTC TCTTCATGGG TACAATGTGT GGCGAGACCC TCAGAAGCCC 
    ACCCAGATAC TTGCAAAGTT GTGTAAGGAG GGCAAACTGG ATGGACCGCA TTATGGCCCA 
    GGTGGAAAGG TTAAAGTTGC AAACCGCATC TTTCTCGGGC CAACGGATAT TGAAGATGAA 
    AATGGTCTAA AGAAGCAAAC AGAAGAGCAT TTGGCTCTTA CTGCACTGAA ACACTGGGAG 
    GAAATTCCTC GAGTGGGCTG CAAACTCATC CCAGAACATG TAGAAACCCG ACCACTCCTC 
    AACCCAGATA AACCTGGAAT AGAGCAGGGT CGGCTTGAGA TGTGGGTGGA TATGTTCCCA 
    ATGGATGTTC CTTCACCAGG ACCAGCCATA GACATATCAC CACGGAAACC AAAGAGATAT 
    GAGCTCAGGG TGATAATATG GAATACTGAC GAGGTAATTC TGGAAGACGA TGATTACTTC 
    ACAGGGGAAA AGTCCAGTGA CATTTTTGTC AGGGGCTGGC TGAAAGGACA GCAGGAAGAT 
    AAGCAGGACA CAGACGTTCA CTACCACTCT CTGACAGGAG AGGGAAACTT CAACTGGCGC 
    TTTGTGTTTC CCTTCGACTA TCTCATGGCT GAGGAGAAAA TTGTGATCTC TAAGAAAGAG 
    TCTTTGTTCT CCTGGGACGA GACAGAGTAC AAGATCCCTG CCCGGCTCAC CATGCAAGTG 
    TGGGACGCTG ACCACTTCTC TGCTGATGAC TTCTTGGGTG CGATTGAGCT GGACTTGAAC 
    CGGTTTCCTC GGGGAGCAAA AACAGCAAAG CAGTGCTCCA TCGACATGGT GCTTAATGAG 
    CAAGAGCTGC CAACCATCTC AATCTTCAAA CAGAAAAGGG TGAAGGGCTG GTGGCCTTTT 
    GTGGCGCGAG ATGAGAATGA TGACCTGGAG CTGACGGGAA AAGTGGAGGC AGAGCTTCAT 
    CTAATGACAG CAGAGGAAGC AGAGAAGAGT CCTGTGGGCC TTGGAAGGAA TGAACCAGAC 
    CCACTGGAGA AACCAAACCG GCCCGACACC ACCTTCCTCT GGTTCCTGAG TCCACTGAAG 
    GCCATCCGCT ACCTGGTGTG CAACCGCTAC AAATGGCTCA TAATCAAGAT TGTCCTGGCC 
    CTGCTGCTGC TGATCATGGT GGGCCTCTTC CTCTACAGCA TGCCTGGCTA CCTGGTCAAG 
    AAGCTGCTGG GAGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    otof ( XM_007260867.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007260929.1 Astyanax mexicanus otoferlin (otof), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OAs42393
    Clone ID Related Accession (Same CDS sequence) XM_007260867.2
    Accession Version XM_007260867.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1455033600000
    Organism Astyanax mexicanus(Mexican tetra)
    Product otoferlin isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749495.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007260867.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TTGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGACGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTCTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCGGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCCTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA GCTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATCCCTTA CGACGATGAG GAGATCAGAA GGGCTCTTAT TGCTGTCCAT 
    GACTTTGCTG TCCCTCAGAT CAAGATTGGT CCAGCTGGAC GGACAGAGCT TCCTCCCATT 
    GATGGGCCAA CTGATGTTGA CCGAGGTCCC ATCTTACCTG TGCCTCTGGG CATACGGCCC 
    GTCCTCAGCA GATACCGCAT AGAGGTCTTG TTCTGGGGGC TGCGGGACCT GAAGAGAATT 
    AACCTGGCGC AGGTCGACAG GCCTCGAGTG GACATTGAGT GTGCAGGCAA AGGAATCCAA 
    TCAGCTCTCA TTCAGAATTA CAAGAAAAAC CCAAATTTCA GCACCCTGGT CAAGTGGTTT 
    GAAGTGGATC TGCCAGAGAA CGAGTTGCTT CATCCTCCTC TGAATATTCG GGTGGTAGAC 
    TGCAGAGCAT TTGGCCGCTA CACATTGGTG GGCTCACATG CTGTAACCAG TCTGCGCAAG 
    TTCATCTACA TCCCTCCTGA CAAAAGGTCC AATAACTGGG CTAATACAGC TAGACTGGCC 
    AATGGCTACT TGGCTCTCAC CAATGGGACC TCCCGTTCCC GCCCCTACTC CCGCCCCATC 
    TCTCACTCCT CAGGTGACAT CGTGGTCAAC ATGGACTCTG AGCCCAATGT CAAGAAGATG 
    GAGACTGTTG TAAAACTGGA TGCTACCTCT GATGCTGTTG TTAAAATTGA TGTGAATGAG 
    GAAGAAAAGG AAAAAGACAA AAAGAAGAAA AAGAAAAAGA AGGGAGAGGA GATAGAAGAT 
    GAGGAGCCAG ATGAGAGCAT GCTGGACTGG TGGTCAAAGT ACTTTGCTTC AATAGAGACC 
    ATGATGGAGA ACCTACGAGT CCAGGAAGCA GCATGGGCTG AGGCTGAGGA GAGAGAAGAT 
    TTGGAGATTG CGGCAGAGGG AGCAGAAATC AAAAGTGATG ACTCTTGTAC AAAAGGCACG 
    AAGCCCAAAG AGAAGAACAA GATAAAGAGC TCAAAGGACA AGAAGAAGAG CCACGGCAGT 
    GATGGGACAG AGAAAAAAGT GCCCAAACCC AAAGTAGACG AGCTTTTGGT CTACAATAAG 
    GAGCTGGAGA CTGAGTTTGA TCACTTTGAA GACTGGCTTC ACACCTTCAA CCTGTACAGA 
    GGGAAGGCCA TAGATGATGA TGACCAAAAC ATTGTGGATG ACGACAGGAT TGTGGGCCGA 
    TTTAAAGGGT CATTGTGCAT GTACAAGCTG CCTCTGTCAG ATGAAATCAC CAGAGATGTT 
    GGATTCGATC CAAATATGGG CATGTTTCAG AGTATCCCAC ACAATGATCC CATCAATGTA 
    CTTGTGAGGG TTTATGTTAT CAGGGCAACA GACCTTCACC CTGCAGATAT AAACGGAAAA 
    GCTGATCCAT ATGTTGTTAT CAAGCTGGGA AAGTCAGAGA TCAAGGACAA GGAGAACTAC 
    ATCTCTAAAC AGCTGAATCC TGTCTTTGGG AAGTCCTTTG ACATTGAAGC CACGTTTCCA 
    ATGGAGTCTA TGCTGACAGT CTCAGTGTAC GACTGGGATT TAGTTGGCAC TGATGATTTG 
    ATCGGAGAGA CCAAGATTGA CTTGGAGAAC CGTTACTACA GCAAACACAG AGCCACCTGT 
    GGCATCGCAT CCAATTACTC TCTTCATGGG TACAATGTGT GGCGAGACCC TCAGAAGCCC 
    ACCCAGATAC TTGCAAAGTT GTGTAAGGAG GGCAAACTGG ATGGACCGCA TTATGGCCCA 
    GGTGGAAAGG TTAAAGTTGC AAACCGCATC TTTCTCGGGC CAACGGATAT TGAAGATGAA 
    AATGGTCTAA AGAAGCAAAC AGAAGAGCAT TTGGCTCTTA CTGCACTGAA ACACTGGGAG 
    GAAATTCCTC GAGTGGGCTG CAAACTCATC CCAGAACATG TAGAAACCCG ACCACTCCTC 
    AACCCAGATA AACCTGGAAT AGAGCAGGGT CGGCTTGAGA TGTGGGTGGA TATGTTCCCA 
    ATGGATGTTC CTTCACCAGG ACCAGCCATA GACATATCAC CACGGAAACC AAAGAGCTTT 
    GAGCTTCGTG TTATTATTTG GAACACTGAT GACGTAATTC TAGAAGACGA TGCTTTCATG 
    ACAGGAGAGA AGATGTCTGA CATCTATGTC AGGGGCTGGC TGAAAGGACA GCAGGAAGAT 
    AAGCAGGACA CAGACGTTCA CTACCACTCT CTGACAGGAG AGGGAAACTT CAACTGGCGC 
    TTTGTGTTTC CCTTCGACTA TCTCATGGCT GAGGAGAAAA TTGTGATCTC TAAGAAAGAG 
    TCTTTGTTCT CCTGGGACGA GACAGAGTAC AAGATCCCTG CCCGGCTCAC CATGCAAGTG 
    TGGGACGCTG ACCACTTCTC TGCTGATGAC TTCTTGGGTG CGATTGAGCT GGACTTGAAC 
    CGGTTTCCTC GGGGAGCAAA AACAGCAAAG CAGTGCTCCA TCGACATGGT GCTTAATGAG 
    CAAGAGCTGC CAACCATCTC AATCTTCAAA CAGAAAAGGG TGAAGGGCTG GTGGCCTTTT 
    GTGGCGCGAG ATGAGAATGA TGACCTGGAG CTGACGGGAA AAGTGGAGGC AGAGCTTCAT 
    CTAATGACAG CAGAGGAAGC AGAGAAGAGT CCTGTGGGCC TTGGAAGGAA TGAACCAGAC 
    CCACTGGAGA AACCAAATCG CCCGGACACC AGTCTCATGT GGTTCATGAA CCCCCTGAAG 
    TCCCTGCGAT ACTTCATTTG GCACAACTAC CGCTGGCTGA TCCTGAAGAC CCTAGGGCTG 
    ATGCTGCTCC TCCTGCTCCT CGGCCTCTTT CTCTACTCCA TCCCTGGATA CCTGGTCAAG 
    AAGCTGCTGG GGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_007260929.1
    CDS134..6271
    Translation

    Target ORF information:

    RefSeq Version XM_007260867.2
    Organism Astyanax mexicanus(Mexican tetra)
    Definition Astyanax mexicanus otoferlin (otof), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_007260867.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTTAG TTGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGACGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTCTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCGGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCCTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA GCTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATCCCTTA CGACGATGAG GAGATCAGAA GGGCTCTTAT TGCTGTCCAT 
    GACTTTGCTG TCCCTCAGAT CAAGATTGGT CCAGCTGGAC GGACAGAGCT TCCTCCCATT 
    GATGGGCCAA CTGATGTTGA CCGAGGTCCC ATCTTACCTG TGCCTCTGGG CATACGGCCC 
    GTCCTCAGCA GATACCGCAT AGAGGTCTTG TTCTGGGGGC TGCGGGACCT GAAGAGAATT 
    AACCTGGCGC AGGTCGACAG GCCTCGAGTG GACATTGAGT GTGCAGGCAA AGGAATCCAA 
    TCAGCTCTCA TTCAGAATTA CAAGAAAAAC CCAAATTTCA GCACCCTGGT CAAGTGGTTT 
    GAAGTGGATC TGCCAGAGAA CGAGTTGCTT CATCCTCCTC TGAATATTCG GGTGGTAGAC 
    TGCAGAGCAT TTGGCCGCTA CACATTGGTG GGCTCACATG CTGTAACCAG TCTGCGCAAG 
    TTCATCTACA TCCCTCCTGA CAAAAGGTCC AATAACTGGG CTAATACAGC TAGACTGGCC 
    AATGGCTACT TGGCTCTCAC CAATGGGACC TCCCGTTCCC GCCCCTACTC CCGCCCCATC 
    TCTCACTCCT CAGGTGACAT CGTGGTCAAC ATGGACTCTG AGCCCAATGT CAAGAAGATG 
    GAGACTGTTG TAAAACTGGA TGCTACCTCT GATGCTGTTG TTAAAATTGA TGTGAATGAG 
    GAAGAAAAGG AAAAAGACAA AAAGAAGAAA AAGAAAAAGA AGGGAGAGGA GATAGAAGAT 
    GAGGAGCCAG ATGAGAGCAT GCTGGACTGG TGGTCAAAGT ACTTTGCTTC AATAGAGACC 
    ATGATGGAGA ACCTACGAGT CCAGGAAGCA GCATGGGCTG AGGCTGAGGA GAGAGAAGAT 
    TTGGAGATTG CGGCAGAGGG AGCAGAAATC AAAAGTGATG ACTCTTGTAC AAAAGGCACG 
    AAGCCCAAAG AGAAGAACAA GATAAAGAGC TCAAAGGACA AGAAGAAGAG CCACGGCAGT 
    GATGGGACAG AGAAAAAAGT GCCCAAACCC AAAGTAGACG AGCTTTTGGT CTACAATAAG 
    GAGCTGGAGA CTGAGTTTGA TCACTTTGAA GACTGGCTTC ACACCTTCAA CCTGTACAGA 
    GGGAAGGCCA TAGATGATGA TGACCAAAAC ATTGTGGATG ACGACAGGAT TGTGGGCCGA 
    TTTAAAGGGT CATTGTGCAT GTACAAGCTG CCTCTGTCAG ATGAAATCAC CAGAGATGTT 
    GGATTCGATC CAAATATGGG CATGTTTCAG AGTATCCCAC ACAATGATCC CATCAATGTA 
    CTTGTGAGGG TTTATGTTAT CAGGGCAACA GACCTTCACC CTGCAGATAT AAACGGAAAA 
    GCTGATCCAT ATGTTGTTAT CAAGCTGGGA AAGTCAGAGA TCAAGGACAA GGAGAACTAC 
    ATCTCTAAAC AGCTGAATCC TGTCTTTGGG AAGTCCTTTG ACATTGAAGC CACGTTTCCA 
    ATGGAGTCTA TGCTGACAGT CTCAGTGTAC GACTGGGATT TAGTTGGCAC TGATGATTTG 
    ATCGGAGAGA CCAAGATTGA CTTGGAGAAC CGTTACTACA GCAAACACAG AGCCACCTGT 
    GGCATCGCAT CCAATTACTC TCTTCATGGG TACAATGTGT GGCGAGACCC TCAGAAGCCC 
    ACCCAGATAC TTGCAAAGTT GTGTAAGGAG GGCAAACTGG ATGGACCGCA TTATGGCCCA 
    GGTGGAAAGG TTAAAGTTGC AAACCGCATC TTTCTCGGGC CAACGGATAT TGAAGATGAA 
    AATGGTCTAA AGAAGCAAAC AGAAGAGCAT TTGGCTCTTA CTGCACTGAA ACACTGGGAG 
    GAAATTCCTC GAGTGGGCTG CAAACTCATC CCAGAACATG TAGAAACCCG ACCACTCCTC 
    AACCCAGATA AACCTGGAAT AGAGCAGGGT CGGCTTGAGA TGTGGGTGGA TATGTTCCCA 
    ATGGATGTTC CTTCACCAGG ACCAGCCATA GACATATCAC CACGGAAACC AAAGAGCTTT 
    GAGCTTCGTG TTATTATTTG GAACACTGAT GACGTAATTC TAGAAGACGA TGCTTTCATG 
    ACAGGAGAGA AGATGTCTGA CATCTATGTC AGGGGCTGGC TGAAAGGACA GCAGGAAGAT 
    AAGCAGGACA CAGACGTTCA CTACCACTCT CTGACAGGAG AGGGAAACTT CAACTGGCGC 
    TTTGTGTTTC CCTTCGACTA TCTCATGGCT GAGGAGAAAA TTGTGATCTC TAAGAAAGAG 
    TCTTTGTTCT CCTGGGACGA GACAGAGTAC AAGATCCCTG CCCGGCTCAC CATGCAAGTG 
    TGGGACGCTG ACCACTTCTC TGCTGATGAC TTCTTGGGTG CGATTGAGCT GGACTTGAAC 
    CGGTTTCCTC GGGGAGCAAA AACAGCAAAG CAGTGCTCCA TCGACATGGT GCTTAATGAG 
    CAAGAGCTGC CAACCATCTC AATCTTCAAA CAGAAAAGGG TGAAGGGCTG GTGGCCTTTT 
    GTGGCGCGAG ATGAGAATGA TGACCTGGAG CTGACGGGAA AAGTGGAGGC AGAGCTTCAT 
    CTAATGACAG CAGAGGAAGC AGAGAAGAGT CCTGTGGGCC TTGGAAGGAA TGAACCAGAC 
    CCACTGGAGA AACCAAATCG CCCGGACACC AGTCTCATGT GGTTCATGAA CCCCCTGAAG 
    TCCCTGCGAT ACTTCATTTG GCACAACTAC CGCTGGCTGA TCCTGAAGAC CCTAGGGCTG 
    ATGCTGCTCC TCCTGCTCCT CGGCCTCTTT CTCTACTCCA TCCCTGGATA CCTGGTCAAG 
    AAGCTGCTGG GGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    otof ( XM_007260873.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007260935.1 Astyanax mexicanus otoferlin (otof), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID OAs42395
    Clone ID Related Accession (Same CDS sequence) XM_007260873.2
    Accession Version XM_007260873.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6066bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1455033600000
    Organism Astyanax mexicanus(Mexican tetra)
    Product otoferlin isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749495.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007260873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TTGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGACGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTCTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCGGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCCTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA GCTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATTGGTCC AGCTGGACGG ACAGAGCTTC CTCCCATTGA TGGGCCAACT 
    GATGTTGACC GAGGTCCCAT CTTACCTGTG CCTCTGGGCA TACGGCCCGT CCTCAGCAGA 
    TACCGCATAG AGGTCTTGTT CTGGGGGCTG CGGGACCTGA AGAGAATTAA CCTGGCGCAG 
    GTCGACAGGC CTCGAGTGGA CATTGAGTGT GCAGGCAAAG GAATCCAATC AGCTCTCATT 
    CAGAATTACA AGAAAAACCC AAATTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGATCTG 
    CCAGAGAACG AGTTGCTTCA TCCTCCTCTG AATATTCGGG TGGTAGACTG CAGAGCATTT 
    GGCCGCTACA CATTGGTGGG CTCACATGCT GTAACCAGTC TGCGCAAGTT CATCTACATC 
    CCTCCTGACA AAAGGTCCAA TAACTGGGCT AATACAGCTA GACTGGCCAA TGGCTACTTG 
    GCTCTCACCA ATGGGACCTC CCGTTCCCGC CCCTACTCCC GCCCCATCTC TCACTCCTCA 
    GGTGACATCG TGGTCAACAT GGACTCTGAG CCCAATGTCA AGAAGATGGA GACTGTTGTA 
    AAACTGGATG CTACCTCTGA TGCTGTTGTT AAAATTGATG TGAATGAGGA AGAAAAGGAA 
    AAAGACAAAA AGAAGAAAAA GAAAAAGAAG GGAGAGGAGA TAGAAGATGA GGAGCCAGAT 
    GAGAGCATGC TGGACTGGTG GTCAAAGTAC TTTGCTTCAA TAGAGACCAT GATGGAGAAC 
    CTACGAGTCC AGGAAGCAGC ATGGGCTGAG GCTGAGGAGA GAGAAGATTT GGAGATTGCG 
    GCAGAGGGAG CAGAAATCAA AAGTGATGAC TCTTGTACAA AAGGCACGAA GCCCAAAGAG 
    AAGAACAAGA TAAAGAGCTC AAAGGACAAG AAGAAGAGCC ACGGCAGTGA TGGGACAGAG 
    AAAAAAGTGC CCAAACCCAA AGTAGACGAG CTTTTGGTCT ACAATAAGGA GCTGGAGACT 
    GAGTTTGATC ACTTTGAAGA CTGGCTTCAC ACCTTCAACC TGTACAGAGG GAAGGCCATA 
    GATGATGATG ACCAAAACAT TGTGGATGAC GACAGGATTG TGGGCCGATT TAAAGGGTCA 
    TTGTGCATGT ACAAGCTGCC TCTGTCAGAT GAAATCACCA GAGATGTTGG ATTCGATCCA 
    AATATGGGCA TGTTTCAGAG TATCCCACAC AATGATCCCA TCAATGTACT TGTGAGGGTT 
    TATGTTATCA GGGCAACAGA CCTTCACCCT GCAGATATAA ACGGAAAAGC TGATCCATAT 
    GTTGTTATCA AGCTGGGAAA GTCAGAGATC AAGGACAAGG AGAACTACAT CTCTAAACAG 
    CTGAATCCTG TCTTTGGGAA GTCCTTTGAC ATTGAAGCCA CGTTTCCAAT GGAGTCTATG 
    CTGACAGTCT CAGTGTACGA CTGGGATTTA GTTGGCACTG ATGATTTGAT CGGAGAGACC 
    AAGATTGACT TGGAGAACCG TTACTACAGC AAACACAGAG CCACCTGTGG CATCGCATCC 
    AATTACTCTC TTCATGGGTA CAATGTGTGG CGAGACCCTC AGAAGCCCAC CCAGATACTT 
    GCAAAGTTGT GTAAGGAGGG CAAACTGGAT GGACCGCATT ATGGCCCAGG TGGAAAGGTT 
    AAAGTTGCAA ACCGCATCTT TCTCGGGCCA ACGGATATTG AAGATGAAAA TGGTCTAAAG 
    AAGCAAACAG AAGAGCATTT GGCTCTTACT GCACTGAAAC ACTGGGAGGA AATTCCTCGA 
    GTGGGCTGCA AACTCATCCC AGAACATGTA GAAACCCGAC CACTCCTCAA CCCAGATAAA 
    CCTGGAATAG AGCAGGGTCG GCTTGAGATG TGGGTGGATA TGTTCCCAAT GGATGTTCCT 
    TCACCAGGAC CAGCCATAGA CATATCACCA CGGAAACCAA AGAGATATGA GCTCAGGGTG 
    ATAATATGGA ATACTGACGA GGTAATTCTG GAAGACGATG ATTACTTCAC AGGGGAAAAG 
    TCCAGTGACA TTTTTGTCAG GGGCTGGCTG AAAGGACAGC AGGAAGATAA GCAGGACACA 
    GACGTTCACT ACCACTCTCT GACAGGAGAG GGAAACTTCA ACTGGCGCTT TGTGTTTCCC 
    TTCGACTATC TCATGGCTGA GGAGAAAATT GTGATCTCTA AGAAAGAGTC TTTGTTCTCC 
    TGGGACGAGA CAGAGTACAA GATCCCTGCC CGGCTCACCA TGCAAGTGTG GGACGCTGAC 
    CACTTCTCTG CTGATGACTT CTTGGGTGCG ATTGAGCTGG ACTTGAACCG GTTTCCTCGG 
    GGAGCAAAAA CAGCAAAGCA GTGCTCCATC GACATGGTGC TTAATGAGCA AGAGCTGCCA 
    ACCATCTCAA TCTTCAAACA GAAAAGGGTG AAGGGCTGGT GGCCTTTTGT GGCGCGAGAT 
    GAGAATGATG ACCTGGAGCT GACGGGAAAA GTGGAGGCAG AGCTTCATCT AATGACAGCA 
    GAGGAAGCAG AGAAGAGTCC TGTGGGCCTT GGAAGGAATG AACCAGACCC ACTGGAGAAA 
    CCAAATCGCC CGGACACCAG TCTCATGTGG TTCATGAACC CCCTGAAGTC CCTGCGATAC 
    TTCATTTGGC ACAACTACCG CTGGCTGATC CTGAAGACCC TAGGGCTGAT GCTGCTCCTC 
    CTGCTCCTCG GCCTCTTTCT CTACTCCATC CCTGGATACC TGGTCAAGAA GCTGCTGGGG 
    GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_007260935.1
    CDS134..6199
    Translation

    Target ORF information:

    RefSeq Version XM_007260873.2
    Organism Astyanax mexicanus(Mexican tetra)
    Definition Astyanax mexicanus otoferlin (otof), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_007260873.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TTGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGACGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTCTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCGGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCCTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA GCTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATTGGTCC AGCTGGACGG ACAGAGCTTC CTCCCATTGA TGGGCCAACT 
    GATGTTGACC GAGGTCCCAT CTTACCTGTG CCTCTGGGCA TACGGCCCGT CCTCAGCAGA 
    TACCGCATAG AGGTCTTGTT CTGGGGGCTG CGGGACCTGA AGAGAATTAA CCTGGCGCAG 
    GTCGACAGGC CTCGAGTGGA CATTGAGTGT GCAGGCAAAG GAATCCAATC AGCTCTCATT 
    CAGAATTACA AGAAAAACCC AAATTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGATCTG 
    CCAGAGAACG AGTTGCTTCA TCCTCCTCTG AATATTCGGG TGGTAGACTG CAGAGCATTT 
    GGCCGCTACA CATTGGTGGG CTCACATGCT GTAACCAGTC TGCGCAAGTT CATCTACATC 
    CCTCCTGACA AAAGGTCCAA TAACTGGGCT AATACAGCTA GACTGGCCAA TGGCTACTTG 
    GCTCTCACCA ATGGGACCTC CCGTTCCCGC CCCTACTCCC GCCCCATCTC TCACTCCTCA 
    GGTGACATCG TGGTCAACAT GGACTCTGAG CCCAATGTCA AGAAGATGGA GACTGTTGTA 
    AAACTGGATG CTACCTCTGA TGCTGTTGTT AAAATTGATG TGAATGAGGA AGAAAAGGAA 
    AAAGACAAAA AGAAGAAAAA GAAAAAGAAG GGAGAGGAGA TAGAAGATGA GGAGCCAGAT 
    GAGAGCATGC TGGACTGGTG GTCAAAGTAC TTTGCTTCAA TAGAGACCAT GATGGAGAAC 
    CTACGAGTCC AGGAAGCAGC ATGGGCTGAG GCTGAGGAGA GAGAAGATTT GGAGATTGCG 
    GCAGAGGGAG CAGAAATCAA AAGTGATGAC TCTTGTACAA AAGGCACGAA GCCCAAAGAG 
    AAGAACAAGA TAAAGAGCTC AAAGGACAAG AAGAAGAGCC ACGGCAGTGA TGGGACAGAG 
    AAAAAAGTGC CCAAACCCAA AGTAGACGAG CTTTTGGTCT ACAATAAGGA GCTGGAGACT 
    GAGTTTGATC ACTTTGAAGA CTGGCTTCAC ACCTTCAACC TGTACAGAGG GAAGGCCATA 
    GATGATGATG ACCAAAACAT TGTGGATGAC GACAGGATTG TGGGCCGATT TAAAGGGTCA 
    TTGTGCATGT ACAAGCTGCC TCTGTCAGAT GAAATCACCA GAGATGTTGG ATTCGATCCA 
    AATATGGGCA TGTTTCAGAG TATCCCACAC AATGATCCCA TCAATGTACT TGTGAGGGTT 
    TATGTTATCA GGGCAACAGA CCTTCACCCT GCAGATATAA ACGGAAAAGC TGATCCATAT 
    GTTGTTATCA AGCTGGGAAA GTCAGAGATC AAGGACAAGG AGAACTACAT CTCTAAACAG 
    CTGAATCCTG TCTTTGGGAA GTCCTTTGAC ATTGAAGCCA CGTTTCCAAT GGAGTCTATG 
    CTGACAGTCT CAGTGTACGA CTGGGATTTA GTTGGCACTG ATGATTTGAT CGGAGAGACC 
    AAGATTGACT TGGAGAACCG TTACTACAGC AAACACAGAG CCACCTGTGG CATCGCATCC 
    AATTACTCTC TTCATGGGTA CAATGTGTGG CGAGACCCTC AGAAGCCCAC CCAGATACTT 
    GCAAAGTTGT GTAAGGAGGG CAAACTGGAT GGACCGCATT ATGGCCCAGG TGGAAAGGTT 
    AAAGTTGCAA ACCGCATCTT TCTCGGGCCA ACGGATATTG AAGATGAAAA TGGTCTAAAG 
    AAGCAAACAG AAGAGCATTT GGCTCTTACT GCACTGAAAC ACTGGGAGGA AATTCCTCGA 
    GTGGGCTGCA AACTCATCCC AGAACATGTA GAAACCCGAC CACTCCTCAA CCCAGATAAA 
    CCTGGAATAG AGCAGGGTCG GCTTGAGATG TGGGTGGATA TGTTCCCAAT GGATGTTCCT 
    TCACCAGGAC CAGCCATAGA CATATCACCA CGGAAACCAA AGAGATATGA GCTCAGGGTG 
    ATAATATGGA ATACTGACGA GGTAATTCTG GAAGACGATG ATTACTTCAC AGGGGAAAAG 
    TCCAGTGACA TTTTTGTCAG GGGCTGGCTG AAAGGACAGC AGGAAGATAA GCAGGACACA 
    GACGTTCACT ACCACTCTCT GACAGGAGAG GGAAACTTCA ACTGGCGCTT TGTGTTTCCC 
    TTCGACTATC TCATGGCTGA GGAGAAAATT GTGATCTCTA AGAAAGAGTC TTTGTTCTCC 
    TGGGACGAGA CAGAGTACAA GATCCCTGCC CGGCTCACCA TGCAAGTGTG GGACGCTGAC 
    CACTTCTCTG CTGATGACTT CTTGGGTGCG ATTGAGCTGG ACTTGAACCG GTTTCCTCGG 
    GGAGCAAAAA CAGCAAAGCA GTGCTCCATC GACATGGTGC TTAATGAGCA AGAGCTGCCA 
    ACCATCTCAA TCTTCAAACA GAAAAGGGTG AAGGGCTGGT GGCCTTTTGT GGCGCGAGAT 
    GAGAATGATG ACCTGGAGCT GACGGGAAAA GTGGAGGCAG AGCTTCATCT AATGACAGCA 
    GAGGAAGCAG AGAAGAGTCC TGTGGGCCTT GGAAGGAATG AACCAGACCC ACTGGAGAAA 
    CCAAATCGCC CGGACACCAG TCTCATGTGG TTCATGAACC CCCTGAAGTC CCTGCGATAC 
    TTCATTTGGC ACAACTACCG CTGGCTGATC CTGAAGACCC TAGGGCTGAT GCTGCTCCTC 
    CTGCTCCTCG GCCTCTTTCT CTACTCCATC CCTGGATACC TGGTCAAGAA GCTGCTGGGG 
    GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    otof ( XM_007260872.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007260934.1 Astyanax mexicanus otoferlin (otof), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID OAs42396
    Clone ID Related Accession (Same CDS sequence) XM_007260872.2
    Accession Version XM_007260872.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6066bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1455033600000
    Organism Astyanax mexicanus(Mexican tetra)
    Product otoferlin isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749495.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007260872.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TTGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGACGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTCTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCGGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCCTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA GCTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATTGGTCC AGCTGGACGG ACAGAGCTTC CTCCCATTGA TGGGCCAACT 
    GATGTTGACC GAGGTCCCAT CTTACCTGTG CCTCTGGGCA TACGGCCCGT CCTCAGCAGA 
    TACCGCATAG AGGTCTTGTT CTGGGGGCTG CGGGACCTGA AGAGAATTAA CCTGGCGCAG 
    GTCGACAGGC CTCGAGTGGA CATTGAGTGT GCAGGCAAAG GAATCCAATC AGCTCTCATT 
    CAGAATTACA AGAAAAACCC AAATTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGATCTG 
    CCAGAGAACG AGTTGCTTCA TCCTCCTCTG AATATTCGGG TGGTAGACTG CAGAGCATTT 
    GGCCGCTACA CATTGGTGGG CTCACATGCT GTAACCAGTC TGCGCAAGTT CATCTACATC 
    CCTCCTGACA AAAGGTCCAA TAACTGGGCT AATACAGCTA GACTGGCCAA TGGCTACTTG 
    GCTCTCACCA ATGGGACCTC CCGTTCCCGC CCCTACTCCC GCCCCATCTC TCACTCCTCA 
    GGTGACATCG TGGTCAACAT GGACTCTGAG CCCAATGTCA AGAAGATGGA GACTGTTGTA 
    AAACTGGATG CTACCTCTGA TGCTGTTGTT AAAATTGATG TGAATGAGGA AGAAAAGGAA 
    AAAGACAAAA AGAAGAAAAA GAAAAAGAAG GGAGAGGAGA TAGAAGATGA GGAGCCAGAT 
    GAGAGCATGC TGGACTGGTG GTCAAAGTAC TTTGCTTCAA TAGAGACCAT GATGGAGAAC 
    CTACGAGTCC AGGAAGCAGC ATGGGCTGAG GCTGAGGAGA GAGAAGATTT GGAGATTGCG 
    GCAGAGGGAG CAGAAATCAA AAGTGATGAC TCTTGTACAA AAGGCACGAA GCCCAAAGAG 
    AAGAACAAGA TAAAGAGCTC AAAGGACAAG AAGAAGAGCC ACGGCAGTGA TGGGACAGAG 
    AAAAAAGTGC CCAAACCCAA AGTAGACGAG CTTTTGGTCT ACAATAAGGA GCTGGAGACT 
    GAGTTTGATC ACTTTGAAGA CTGGCTTCAC ACCTTCAACC TGTACAGAGG GAAGGCCATA 
    GATGATGATG ACCAAAACAT TGTGGATGAC GACAGGATTG TGGGCCGATT TAAAGGGTCA 
    TTGTGCATGT ACAAGCTGCC TCTGTCAGAT GAAATCACCA GAGATGTTGG ATTCGATCCA 
    AATATGGGCA TGTTTCAGAG TATCCCACAC AATGATCCCA TCAATGTACT TGTGAGGGTT 
    TATGTTATCA GGGCAACAGA CCTTCACCCT GCAGATATAA ACGGAAAAGC TGATCCATAT 
    GTTGTTATCA AGCTGGGAAA GTCAGAGATC AAGGACAAGG AGAACTACAT CTCTAAACAG 
    CTGAATCCTG TCTTTGGGAA GTCCTTTGAC ATTGAAGCCA CGTTTCCAAT GGAGTCTATG 
    CTGACAGTCT CAGTGTACGA CTGGGATTTA GTTGGCACTG ATGATTTGAT CGGAGAGACC 
    AAGATTGACT TGGAGAACCG TTACTACAGC AAACACAGAG CCACCTGTGG CATCGCATCC 
    AATTACTCTC TTCATGGGTA CAATGTGTGG CGAGACCCTC AGAAGCCCAC CCAGATACTT 
    GCAAAGTTGT GTAAGGAGGG CAAACTGGAT GGACCGCATT ATGGCCCAGG TGGAAAGGTT 
    AAAGTTGCAA ACCGCATCTT TCTCGGGCCA ACGGATATTG AAGATGAAAA TGGTCTAAAG 
    AAGCAAACAG AAGAGCATTT GGCTCTTACT GCACTGAAAC ACTGGGAGGA AATTCCTCGA 
    GTGGGCTGCA AACTCATCCC AGAACATGTA GAAACCCGAC CACTCCTCAA CCCAGATAAA 
    CCTGGAATAG AGCAGGGTCG GCTTGAGATG TGGGTGGATA TGTTCCCAAT GGATGTTCCT 
    TCACCAGGAC CAGCCATAGA CATATCACCA CGGAAACCAA AGAGATATGA GCTCAGGGTG 
    ATAATATGGA ATACTGACGA GGTAATTCTG GAAGACGATG ATTACTTCAC AGGGGAAAAG 
    TCCAGTGACA TTTTTGTCAG GGGCTGGCTG AAAGGACAGC AGGAAGATAA GCAGGACACA 
    GACGTTCACT ACCACTCTCT GACAGGAGAG GGAAACTTCA ACTGGCGCTT TGTGTTTCCC 
    TTCGACTATC TCATGGCTGA GGAGAAAATT GTGATCTCTA AGAAAGAGTC TTTGTTCTCC 
    TGGGACGAGA CAGAGTACAA GATCCCTGCC CGGCTCACCA TGCAAGTGTG GGACGCTGAC 
    CACTTCTCTG CTGATGACTT CTTGGGTGCG ATTGAGCTGG ACTTGAACCG GTTTCCTCGG 
    GGAGCAAAAA CAGCAAAGCA GTGCTCCATC GACATGGTGC TTAATGAGCA AGAGCTGCCA 
    ACCATCTCAA TCTTCAAACA GAAAAGGGTG AAGGGCTGGT GGCCTTTTGT GGCGCGAGAT 
    GAGAATGATG ACCTGGAGCT GACGGGAAAA GTGGAGGCAG AGCTTCATCT AATGACAGCA 
    GAGGAAGCAG AGAAGAGTCC TGTGGGCCTT GGAAGGAATG AACCAGACCC ACTGGAGAAA 
    CCAAACCGGC CCGACACCAC CTTCCTCTGG TTCCTGAGTC CACTGAAGGC CATCCGCTAC 
    CTGGTGTGCA ACCGCTACAA ATGGCTCATA ATCAAGATTG TCCTGGCCCT GCTGCTGCTG 
    ATCATGGTGG GCCTCTTCCT CTACAGCATG CCTGGCTACC TGGTCAAGAA GCTGCTGGGA 
    GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_007260934.1
    CDS134..6199
    Translation

    Target ORF information:

    RefSeq Version XM_007260872.2
    Organism Astyanax mexicanus(Mexican tetra)
    Definition Astyanax mexicanus otoferlin (otof), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_007260872.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TTGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGACGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTCTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCGGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCCTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA GCTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATTGGTCC AGCTGGACGG ACAGAGCTTC CTCCCATTGA TGGGCCAACT 
    GATGTTGACC GAGGTCCCAT CTTACCTGTG CCTCTGGGCA TACGGCCCGT CCTCAGCAGA 
    TACCGCATAG AGGTCTTGTT CTGGGGGCTG CGGGACCTGA AGAGAATTAA CCTGGCGCAG 
    GTCGACAGGC CTCGAGTGGA CATTGAGTGT GCAGGCAAAG GAATCCAATC AGCTCTCATT 
    CAGAATTACA AGAAAAACCC AAATTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGATCTG 
    CCAGAGAACG AGTTGCTTCA TCCTCCTCTG AATATTCGGG TGGTAGACTG CAGAGCATTT 
    GGCCGCTACA CATTGGTGGG CTCACATGCT GTAACCAGTC TGCGCAAGTT CATCTACATC 
    CCTCCTGACA AAAGGTCCAA TAACTGGGCT AATACAGCTA GACTGGCCAA TGGCTACTTG 
    GCTCTCACCA ATGGGACCTC CCGTTCCCGC CCCTACTCCC GCCCCATCTC TCACTCCTCA 
    GGTGACATCG TGGTCAACAT GGACTCTGAG CCCAATGTCA AGAAGATGGA GACTGTTGTA 
    AAACTGGATG CTACCTCTGA TGCTGTTGTT AAAATTGATG TGAATGAGGA AGAAAAGGAA 
    AAAGACAAAA AGAAGAAAAA GAAAAAGAAG GGAGAGGAGA TAGAAGATGA GGAGCCAGAT 
    GAGAGCATGC TGGACTGGTG GTCAAAGTAC TTTGCTTCAA TAGAGACCAT GATGGAGAAC 
    CTACGAGTCC AGGAAGCAGC ATGGGCTGAG GCTGAGGAGA GAGAAGATTT GGAGATTGCG 
    GCAGAGGGAG CAGAAATCAA AAGTGATGAC TCTTGTACAA AAGGCACGAA GCCCAAAGAG 
    AAGAACAAGA TAAAGAGCTC AAAGGACAAG AAGAAGAGCC ACGGCAGTGA TGGGACAGAG 
    AAAAAAGTGC CCAAACCCAA AGTAGACGAG CTTTTGGTCT ACAATAAGGA GCTGGAGACT 
    GAGTTTGATC ACTTTGAAGA CTGGCTTCAC ACCTTCAACC TGTACAGAGG GAAGGCCATA 
    GATGATGATG ACCAAAACAT TGTGGATGAC GACAGGATTG TGGGCCGATT TAAAGGGTCA 
    TTGTGCATGT ACAAGCTGCC TCTGTCAGAT GAAATCACCA GAGATGTTGG ATTCGATCCA 
    AATATGGGCA TGTTTCAGAG TATCCCACAC AATGATCCCA TCAATGTACT TGTGAGGGTT 
    TATGTTATCA GGGCAACAGA CCTTCACCCT GCAGATATAA ACGGAAAAGC TGATCCATAT 
    GTTGTTATCA AGCTGGGAAA GTCAGAGATC AAGGACAAGG AGAACTACAT CTCTAAACAG 
    CTGAATCCTG TCTTTGGGAA GTCCTTTGAC ATTGAAGCCA CGTTTCCAAT GGAGTCTATG 
    CTGACAGTCT CAGTGTACGA CTGGGATTTA GTTGGCACTG ATGATTTGAT CGGAGAGACC 
    AAGATTGACT TGGAGAACCG TTACTACAGC AAACACAGAG CCACCTGTGG CATCGCATCC 
    AATTACTCTC TTCATGGGTA CAATGTGTGG CGAGACCCTC AGAAGCCCAC CCAGATACTT 
    GCAAAGTTGT GTAAGGAGGG CAAACTGGAT GGACCGCATT ATGGCCCAGG TGGAAAGGTT 
    AAAGTTGCAA ACCGCATCTT TCTCGGGCCA ACGGATATTG AAGATGAAAA TGGTCTAAAG 
    AAGCAAACAG AAGAGCATTT GGCTCTTACT GCACTGAAAC ACTGGGAGGA AATTCCTCGA 
    GTGGGCTGCA AACTCATCCC AGAACATGTA GAAACCCGAC CACTCCTCAA CCCAGATAAA 
    CCTGGAATAG AGCAGGGTCG GCTTGAGATG TGGGTGGATA TGTTCCCAAT GGATGTTCCT 
    TCACCAGGAC CAGCCATAGA CATATCACCA CGGAAACCAA AGAGATATGA GCTCAGGGTG 
    ATAATATGGA ATACTGACGA GGTAATTCTG GAAGACGATG ATTACTTCAC AGGGGAAAAG 
    TCCAGTGACA TTTTTGTCAG GGGCTGGCTG AAAGGACAGC AGGAAGATAA GCAGGACACA 
    GACGTTCACT ACCACTCTCT GACAGGAGAG GGAAACTTCA ACTGGCGCTT TGTGTTTCCC 
    TTCGACTATC TCATGGCTGA GGAGAAAATT GTGATCTCTA AGAAAGAGTC TTTGTTCTCC 
    TGGGACGAGA CAGAGTACAA GATCCCTGCC CGGCTCACCA TGCAAGTGTG GGACGCTGAC 
    CACTTCTCTG CTGATGACTT CTTGGGTGCG ATTGAGCTGG ACTTGAACCG GTTTCCTCGG 
    GGAGCAAAAA CAGCAAAGCA GTGCTCCATC GACATGGTGC TTAATGAGCA AGAGCTGCCA 
    ACCATCTCAA TCTTCAAACA GAAAAGGGTG AAGGGCTGGT GGCCTTTTGT GGCGCGAGAT 
    GAGAATGATG ACCTGGAGCT GACGGGAAAA GTGGAGGCAG AGCTTCATCT AATGACAGCA 
    GAGGAAGCAG AGAAGAGTCC TGTGGGCCTT GGAAGGAATG AACCAGACCC ACTGGAGAAA 
    CCAAACCGGC CCGACACCAC CTTCCTCTGG TTCCTGAGTC CACTGAAGGC CATCCGCTAC 
    CTGGTGTGCA ACCGCTACAA ATGGCTCATA ATCAAGATTG TCCTGGCCCT GCTGCTGCTG 
    ATCATGGTGG GCCTCTTCCT CTACAGCATG CCTGGCTACC TGGTCAAGAA GCTGCTGGGA 
    GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    otof ( XM_015608739.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_015464225.1 Astyanax mexicanus otoferlin (otof), transcript variant X9, mRNA.
    Price & Availability↑
    CloneID OAs42398
    Clone ID Related Accession (Same CDS sequence) XM_015608739.1
    Accession Version XM_015608739.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6066bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1455033600000
    Organism Astyanax mexicanus(Mexican tetra)
    Product otoferlin isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749495.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TTGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGACGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTCTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCGGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCCTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA GCTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATTGGTCC AGCTGGACGG ACAGAGCTTC CTCCCATTGA TGGGCCAACT 
    GATGTTGACC GAGGTCCCAT CTTACCTGTG CCTCTGGGCA TACGGCCCGT CCTCAGCAGA 
    TACCGCATAG AGGTCTTGTT CTGGGGGCTG CGGGACCTGA AGAGAATTAA CCTGGCGCAG 
    GTCGACAGGC CTCGAGTGGA CATTGAGTGT GCAGGCAAAG GAATCCAATC AGCTCTCATT 
    CAGAATTACA AGAAAAACCC AAATTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGATCTG 
    CCAGAGAACG AGTTGCTTCA TCCTCCTCTG AATATTCGGG TGGTAGACTG CAGAGCATTT 
    GGCCGCTACA CATTGGTGGG CTCACATGCT GTAACCAGTC TGCGCAAGTT CATCTACATC 
    CCTCCTGACA AAAGGTCCAA TAACTGGGCT AATACAGCTA GACTGGCCAA TGGCTACTTG 
    GCTCTCACCA ATGGGACCTC CCGTTCCCGC CCCTACTCCC GCCCCATCTC TCACTCCTCA 
    GGTGACATCG TGGTCAACAT GGACTCTGAG CCCAATGTCA AGAAGATGGA GACTGTTGTA 
    AAACTGGATG CTACCTCTGA TGCTGTTGTT AAAATTGATG TGAATGAGGA AGAAAAGGAA 
    AAAGACAAAA AGAAGAAAAA GAAAAAGAAG GGAGAGGAGA TAGAAGATGA GGAGCCAGAT 
    GAGAGCATGC TGGACTGGTG GTCAAAGTAC TTTGCTTCAA TAGAGACCAT GATGGAGAAC 
    CTACGAGTCC AGGAAGCAGC ATGGGCTGAG GCTGAGGAGA GAGAAGATTT GGAGATTGCG 
    GCAGAGGGAG CAGAAATCAA AAGTGATGAC TCTTGTACAA AAGGCACGAA GCCCAAAGAG 
    AAGAACAAGA TAAAGAGCTC AAAGGACAAG AAGAAGAGCC ACGGCAGTGA TGGGACAGAG 
    AAAAAAGTGC CCAAACCCAA AGTAGACGAG CTTTTGGTCT ACAATAAGGA GCTGGAGACT 
    GAGTTTGATC ACTTTGAAGA CTGGCTTCAC ACCTTCAACC TGTACAGAGG GAAGGCCATA 
    GATGATGATG ACCAAAACAT TGTGGATGAC GACAGGATTG TGGGCCGATT TAAAGGGTCA 
    TTGTGCATGT ACAAGCTGCC TCTGTCAGAT GAAATCACCA GAGATGTTGG ATTCGATCCA 
    AATATGGGCA TGTTTCAGAG TATCCCACAC AATGATCCCA TCAATGTACT TGTGAGGGTT 
    TATGTTATCA GGGCAACAGA CCTTCACCCT GCAGATATAA ACGGAAAAGC TGATCCATAT 
    GTTGTTATCA AGCTGGGAAA GTCAGAGATC AAGGACAAGG AGAACTACAT CTCTAAACAG 
    CTGAATCCTG TCTTTGGGAA GTCCTTTGAC ATTGAAGCCA CGTTTCCAAT GGAGTCTATG 
    CTGACAGTCT CAGTGTACGA CTGGGATTTA GTTGGCACTG ATGATTTGAT CGGAGAGACC 
    AAGATTGACT TGGAGAACCG TTACTACAGC AAACACAGAG CCACCTGTGG CATCGCATCC 
    AATTACTCTC TTCATGGGTA CAATGTGTGG CGAGACCCTC AGAAGCCCAC CCAGATACTT 
    GCAAAGTTGT GTAAGGAGGG CAAACTGGAT GGACCGCATT ATGGCCCAGG TGGAAAGGTT 
    AAAGTTGCAA ACCGCATCTT TCTCGGGCCA ACGGATATTG AAGATGAAAA TGGTCTAAAG 
    AAGCAAACAG AAGAGCATTT GGCTCTTACT GCACTGAAAC ACTGGGAGGA AATTCCTCGA 
    GTGGGCTGCA AACTCATCCC AGAACATGTA GAAACCCGAC CACTCCTCAA CCCAGATAAA 
    CCTGGAATAG AGCAGGGTCG GCTTGAGATG TGGGTGGATA TGTTCCCAAT GGATGTTCCT 
    TCACCAGGAC CAGCCATAGA CATATCACCA CGGAAACCAA AGAGCTTTGA GCTTCGTGTT 
    ATTATTTGGA ACACTGATGA CGTAATTCTA GAAGACGATG CTTTCATGAC AGGAGAGAAG 
    ATGTCTGACA TCTATGTCAG GGGCTGGCTG AAAGGACAGC AGGAAGATAA GCAGGACACA 
    GACGTTCACT ACCACTCTCT GACAGGAGAG GGAAACTTCA ACTGGCGCTT TGTGTTTCCC 
    TTCGACTATC TCATGGCTGA GGAGAAAATT GTGATCTCTA AGAAAGAGTC TTTGTTCTCC 
    TGGGACGAGA CAGAGTACAA GATCCCTGCC CGGCTCACCA TGCAAGTGTG GGACGCTGAC 
    CACTTCTCTG CTGATGACTT CTTGGGTGCG ATTGAGCTGG ACTTGAACCG GTTTCCTCGG 
    GGAGCAAAAA CAGCAAAGCA GTGCTCCATC GACATGGTGC TTAATGAGCA AGAGCTGCCA 
    ACCATCTCAA TCTTCAAACA GAAAAGGGTG AAGGGCTGGT GGCCTTTTGT GGCGCGAGAT 
    GAGAATGATG ACCTGGAGCT GACGGGAAAA GTGGAGGCAG AGCTTCATCT AATGACAGCA 
    GAGGAAGCAG AGAAGAGTCC TGTGGGCCTT GGAAGGAATG AACCAGACCC ACTGGAGAAA 
    CCAAATCGCC CGGACACCAG TCTCATGTGG TTCATGAACC CCCTGAAGTC CCTGCGATAC 
    TTCATTTGGC ACAACTACCG CTGGCTGATC CTGAAGACCC TAGGGCTGAT GCTGCTCCTC 
    CTGCTCCTCG GCCTCTTTCT CTACTCCATC CCTGGATACC TGGTCAAGAA GCTGCTGGGG 
    GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015464225.1
    CDS134..6199
    Translation

    Target ORF information:

    RefSeq Version XM_015608739.1
    Organism Astyanax mexicanus(Mexican tetra)
    Definition Astyanax mexicanus otoferlin (otof), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_015608739.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTTAG TTGTGTATCT GAAGACGGTG ACGGAGCTCC GCGGGAAGGG GGACCGCATC 
    GCCAAAGTGT CGTTCAGAGG TCTGTCATTC TACTCACGAG TGTTAGAGAA CTGTGAAGAT 
    GAAGCCAGAT TTGAGGAGGC TTTTCGATGG CCAGTGGCCA GTAAAGTGGA TGGAAATGAA 
    ATGCTGGAGA TCCACATATA CAACTACAGC AAAGTATTCA CCAACAGGTT GATTGGCACC 
    TTCCGGATGG TTCTGCAGAA GGTGGTGGAG GAGGGTCACT TGGAGGTGTC AGACACACTC 
    ATAGATGACA ACAACACAGC TATACAGACA AGCATTTCTA TAGAGATCAG ATACCAGCCG 
    ATGGATGGGA GCGTGCGGGT GTGGAGTGAT GGGGAATTCC TCGATGTTCC TGAGGAGCGT 
    GAGATGTTCC AGTTTGAGAC GGAGAGTTTG CTATCCTCAC ACAGTCACAG CTCAGGAACC 
    CCACCAGGAC GGTCAAGCCA CGGCATCCCT GCATTCAGAA AAGCAGGAAA AGGGGTGTTT 
    TCAGCCATGA AGCTTGGAAA GGTACGCAGC TCCAAAGACG ACCACAAGAA AGGAGATGAT 
    GCTGCTATTC TGGAGACGGA GGATCTTGAT CGTAAAGCAA TGCGTCTGGG TGGAGGGCTT 
    GACCCTGATA CAATCTCACT GGCCTCAGTC ACTGCAGTGA CCACTAATGT CTCGAATAAG 
    AGATCAAAGC CTGATATTAA GATGGAGCCT AGTGCTGGAA GACCCGTGGA TTACCAGATT 
    AGTGTCACGG TAATCGAGGC GCGTCAGCTG GTGGGTTTGA ACATGGATCC TGTGGTATGT 
    GTGGAGATTG GAGAAGATAA GAAGTACACG TCCATGAAAG AGTCCACAAA CTGTCCTTAC 
    TACAATGAGT ACTTTGTCTT TGACTTCCAC GTACCACCAG ATGTCATGTT TGACAAGATC 
    CTGAAACTGT CTGTAATTCA TTCTAAAAAC CTGCTGCGGA GTGGCACGCT GGTAGGCACC 
    TTTAAACTTG ATGTGGGAAC AGTCTACTCA CAACCTGAGC ATCAGTTTTA CCACAAATGG 
    GCCATGCTGT CCGACCCTGA TGACATCACG GCTGGGTGCA AAGGCTACAT AAAATGTGAC 
    ATAGCTGTGG TGGGGAAAGG GGACAACATC AAGACTCCAC ACAAAGCCAA CGAGACGGAT 
    GAAGATGATA TAGAAGGCAA TCTGCTGTTG CCTGAGGGTG TTCCGTCAGA GAGGCAGTGG 
    GCTCGCTTCT ATGTGAAGAT CTACCGCGCT GAGGGTCTCC CTAAAATGAA CACCAGCATC 
    ATGGCCAATG TGAAGAAGGC TTTCATTGGA GAGAACAGGG ATCTAGTAGA CCCTTATGTT 
    CAAGTGCAGT TCGCAGGCCA GAAGGGGAAG ACATCAGTGC AGAAGAGCAG CTACGAGCCC 
    ATTTGGAATG AACAGATCAT TTTCACTGAG ATGTTCCCTC CTCTTTGCAA ACGCATGAAA 
    GTTCAGATCA GAGACTCTGA CAAGGTCAAT GACGTCGCCA TAGGAACTCA CTTCATTGAC 
    TTACGCAAGA TTGCAAACGA TGGAGACAAA GGCTTCCTAC CCACTATGGG TCCAGCGTGG 
    GTAAACATGT ACGGCTCTAC GCGTAACTAC ACCCTGATGG ATGAACACCA GGACCTGAAT 
    GAAGGACTCG GAGAGGGGGT GTCCTTCAGG GCCCGCCTCC TTATTAGTGT AGCTGTGGAA 
    ATATTGGACA CCACCTCTCC AGAAATAATG AGCTCCACTG AGGTACAGAT GGAGCCAGTG 
    TCCAATATTT CAGAGAGTGC AACAGGAAAG CTGGAGGAGT TCTTCCTTTT CGGATCATTC 
    CTGGAGGCCA CTATGGTGGA CAGGAAGATC GGCGACAAAC CAATCAACTT TGAAGTTACT 
    ATTGGCAACT ATGGTAACCA GTTAGACGGC GTGAGCAAGC CAGTGTCAGG CAAGAAGAAG 
    AAGAGGGAGG GTGGAGGAGA GAGTGATGAA GAAGAGTCAG AGCTGATCCA GAACTCCAGT 
    GATGAGGAGA CTGAGGATGA TGGGGATCTG GTGTCAGTAT CCTCCACCCC TCCCATGAAA 
    CCTGTGATCA CTGACAGGAA CTACTTTCAC TTGCCGTATT TTGAAAAGAA GCCTTGCATC 
    TACATTAAGA GCTGGTGGCA AGATCAGAGG AGGCGCCTGT ATAACTCGAA CATCATGGAC 
    AAGATTGCGG ATAAACTGGA AGAGGGTCTG AATGATGTTC AGGAGATCAT AAAGACGGAG 
    AAAGCTTATC CTGAACGCAG GCTTAGGGGT GTTCTGGAGG AGCTGAGCAC TGGCTGCAGT 
    CGGTTTGTCA CTCTGGCCAA CAAAGACCAG AACCAGGCTG GCAGAACCAA ACTAGATCGG 
    GAAAGGCTGA AGTCCGGCAT GAGAGAGATG GAGAGCATGG GTCAGCAGGC CAAGACAATT 
    CGCTCCCAAG TGAAGAGAAA CACTGTGAGA GACAAACTCA AACTGGCTCT GAACTTCCTA 
    CAAAGGCTCC GTTTCCTGGC TGATGAGCCT CAGCACAGCA TTCCAGATGT GTTCATATGG 
    ATGATGAGCA ACAATAAGCG CATAGCGTAC GCACGCATTC CTACCAAAGA CATCCTGTTC 
    TCTATAGTAG ATGAGGAGAT GGGCAAAGAC TGTGGAAAGG TTAAAGCTGT CTTCCTCAGG 
    TTGCCTGGAA AGAAGGGCTT CGGTCCGGCT GGCTGGACTG TCCAGGCTAA GCTGGAGATG 
    TACCTGTGGT TGGGCCTGAA TAAACAGAGG AAGGACTTTC TGAGTGGTCT GCCCAACGGT 
    TTTGAGGAGA ATAAAGCAGT GAAAGGCACG GGTCTACAGG CTGTACCTCC CATCAGCCTT 
    ACGTACAACA TGAAGCAGGT GTTCCAGCTG AGAGCTCACA TGTATCAGGC TCGCAGTCTG 
    TTTGCTGCTG ATACTAGTGG ACTTTCTGAC CCTTTTGCAA GAGTATTCTT TTCTACACAC 
    AGCCAGGTTA CAGAGGTGCT CAGTGAAACT CTCTGTCCTA CATGGGACCA GCTGTTGGTG 
    TTTGATAACG TGGAGCTCTA CGGTGAGGCG ACTGAACTGC GAGATGACCC TCCAATCATT 
    GTTATAGAAA TTTATGACCA GGACACTGTG GGGAAAGCAG AGTATATTGG CCGAACTTTT 
    GCCAAGCCGC TCACCAAGAT GGTTGATGAG CATTACGGCC CCCCGCGTTT CCCACCCCAG 
    CTTGAGTACT ATCAGATCTA CAGAGGAAAC TGCACTGCAG GGGAGCTGCT GGCTGCCTTC 
    GAGCTGCTGC AGATTGGTCC AGCTGGACGG ACAGAGCTTC CTCCCATTGA TGGGCCAACT 
    GATGTTGACC GAGGTCCCAT CTTACCTGTG CCTCTGGGCA TACGGCCCGT CCTCAGCAGA 
    TACCGCATAG AGGTCTTGTT CTGGGGGCTG CGGGACCTGA AGAGAATTAA CCTGGCGCAG 
    GTCGACAGGC CTCGAGTGGA CATTGAGTGT GCAGGCAAAG GAATCCAATC AGCTCTCATT 
    CAGAATTACA AGAAAAACCC AAATTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGATCTG 
    CCAGAGAACG AGTTGCTTCA TCCTCCTCTG AATATTCGGG TGGTAGACTG CAGAGCATTT 
    GGCCGCTACA CATTGGTGGG CTCACATGCT GTAACCAGTC TGCGCAAGTT CATCTACATC 
    CCTCCTGACA AAAGGTCCAA TAACTGGGCT AATACAGCTA GACTGGCCAA TGGCTACTTG 
    GCTCTCACCA ATGGGACCTC CCGTTCCCGC CCCTACTCCC GCCCCATCTC TCACTCCTCA 
    GGTGACATCG TGGTCAACAT GGACTCTGAG CCCAATGTCA AGAAGATGGA GACTGTTGTA 
    AAACTGGATG CTACCTCTGA TGCTGTTGTT AAAATTGATG TGAATGAGGA AGAAAAGGAA 
    AAAGACAAAA AGAAGAAAAA GAAAAAGAAG GGAGAGGAGA TAGAAGATGA GGAGCCAGAT 
    GAGAGCATGC TGGACTGGTG GTCAAAGTAC TTTGCTTCAA TAGAGACCAT GATGGAGAAC 
    CTACGAGTCC AGGAAGCAGC ATGGGCTGAG GCTGAGGAGA GAGAAGATTT GGAGATTGCG 
    GCAGAGGGAG CAGAAATCAA AAGTGATGAC TCTTGTACAA AAGGCACGAA GCCCAAAGAG 
    AAGAACAAGA TAAAGAGCTC AAAGGACAAG AAGAAGAGCC ACGGCAGTGA TGGGACAGAG 
    AAAAAAGTGC CCAAACCCAA AGTAGACGAG CTTTTGGTCT ACAATAAGGA GCTGGAGACT 
    GAGTTTGATC ACTTTGAAGA CTGGCTTCAC ACCTTCAACC TGTACAGAGG GAAGGCCATA 
    GATGATGATG ACCAAAACAT TGTGGATGAC GACAGGATTG TGGGCCGATT TAAAGGGTCA 
    TTGTGCATGT ACAAGCTGCC TCTGTCAGAT GAAATCACCA GAGATGTTGG ATTCGATCCA 
    AATATGGGCA TGTTTCAGAG TATCCCACAC AATGATCCCA TCAATGTACT TGTGAGGGTT 
    TATGTTATCA GGGCAACAGA CCTTCACCCT GCAGATATAA ACGGAAAAGC TGATCCATAT 
    GTTGTTATCA AGCTGGGAAA GTCAGAGATC AAGGACAAGG AGAACTACAT CTCTAAACAG 
    CTGAATCCTG TCTTTGGGAA GTCCTTTGAC ATTGAAGCCA CGTTTCCAAT GGAGTCTATG 
    CTGACAGTCT CAGTGTACGA CTGGGATTTA GTTGGCACTG ATGATTTGAT CGGAGAGACC 
    AAGATTGACT TGGAGAACCG TTACTACAGC AAACACAGAG CCACCTGTGG CATCGCATCC 
    AATTACTCTC TTCATGGGTA CAATGTGTGG CGAGACCCTC AGAAGCCCAC CCAGATACTT 
    GCAAAGTTGT GTAAGGAGGG CAAACTGGAT GGACCGCATT ATGGCCCAGG TGGAAAGGTT 
    AAAGTTGCAA ACCGCATCTT TCTCGGGCCA ACGGATATTG AAGATGAAAA TGGTCTAAAG 
    AAGCAAACAG AAGAGCATTT GGCTCTTACT GCACTGAAAC ACTGGGAGGA AATTCCTCGA 
    GTGGGCTGCA AACTCATCCC AGAACATGTA GAAACCCGAC CACTCCTCAA CCCAGATAAA 
    CCTGGAATAG AGCAGGGTCG GCTTGAGATG TGGGTGGATA TGTTCCCAAT GGATGTTCCT 
    TCACCAGGAC CAGCCATAGA CATATCACCA CGGAAACCAA AGAGCTTTGA GCTTCGTGTT 
    ATTATTTGGA ACACTGATGA CGTAATTCTA GAAGACGATG CTTTCATGAC AGGAGAGAAG 
    ATGTCTGACA TCTATGTCAG GGGCTGGCTG AAAGGACAGC AGGAAGATAA GCAGGACACA 
    GACGTTCACT ACCACTCTCT GACAGGAGAG GGAAACTTCA ACTGGCGCTT TGTGTTTCCC 
    TTCGACTATC TCATGGCTGA GGAGAAAATT GTGATCTCTA AGAAAGAGTC TTTGTTCTCC 
    TGGGACGAGA CAGAGTACAA GATCCCTGCC CGGCTCACCA TGCAAGTGTG GGACGCTGAC 
    CACTTCTCTG CTGATGACTT CTTGGGTGCG ATTGAGCTGG ACTTGAACCG GTTTCCTCGG 
    GGAGCAAAAA CAGCAAAGCA GTGCTCCATC GACATGGTGC TTAATGAGCA AGAGCTGCCA 
    ACCATCTCAA TCTTCAAACA GAAAAGGGTG AAGGGCTGGT GGCCTTTTGT GGCGCGAGAT 
    GAGAATGATG ACCTGGAGCT GACGGGAAAA GTGGAGGCAG AGCTTCATCT AATGACAGCA 
    GAGGAAGCAG AGAAGAGTCC TGTGGGCCTT GGAAGGAATG AACCAGACCC ACTGGAGAAA 
    CCAAATCGCC CGGACACCAG TCTCATGTGG TTCATGAACC CCCTGAAGTC CCTGCGATAC 
    TTCATTTGGC ACAACTACCG CTGGCTGATC CTGAAGACCC TAGGGCTGAT GCTGCTCCTC 
    CTGCTCCTCG GCCTCTTTCT CTACTCCATC CCTGGATACC TGGTCAAGAA GCTGCTGGGG 
    GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

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