gas2l1 cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene
Clones
gRNAs

Summary

Gene Symbol	gas2l1
Entrez Gene ID	103028235
Full Name	growth arrest specific 2 like 1
Gene Type	protein-coding
Organism	Astyanax mexicanus(Mexican tetra)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007228771.2	XP_007228833.1	GAS2-like protein 1
XM_007228771.3	XP_007228833.2	GAS2-like protein 1

Pathways from BioSystems
Gene Ontology

The following gas2l1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gas2l1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAs13936	XM_007228771.2 Latest version!	Astyanax mexicanus growth arrest specific 2 like 1 (gas2l1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAs99555	XM_007228771.3 Latest version!	Astyanax mexicanus growth arrest specific 2 like 1 (gas2l1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gas2l1 ( XM_007228771.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007228833.1 Astyanax mexicanus growth arrest specific 2 like 1 (gas2l1), mRNA.

Price & Availability↑

CloneID	OAs13936
Clone ID Related Accession (Same CDS sequence)	XM_007228771.2
Accession Version	XM_007228771.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	GAS2-like protein 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006738779.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007228771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACGG ACCAGAGCAA CATCCAGTCC TCTGCCTGCA AGAGCATCCG GCCGTTCAAG 
TCCAGCGAGG AGTATCTGTA CGCCATGAAG GAGGACCTGG CGGAGTGGCT CCACGGGCTC 
TACGGCCTGG ACATCACCGT GGAAACCTTC ATGGAGAGCC TGGAGACGGG CTGCGTGCTC 
TGTAACCACG CCAACAACGT CAACCGCGCC GCGCGCCCCT TCCAGCCCCA TCGCCCGTGG 
CAGGACGTGG CGTACCAGTC GCGCGGCGCC ATGCCCGGCT CCTTCGTGGC GCGCGACAAC 
GTGTCCAACT TCATCGGCTG GTGCCGCAGC GAGCTGCGCG TGAAGGACGT GCTGATGTTC 
GAGACTAACG ACCTGGTGGA GCGCGGCAGC GAGAAGAACT TCGTGCTCTG CCTCCTGGAG 
GTGGCGCGGA GAGGTGCGCG GTTCGGCGTT CCCGCTCCCG TTCTCGTACA GCTGGAGCAG 
GAGATCGCCC GCGAGGAGAG CCGGGAGCGG GAGCGGGAGA GGGACCTCAA GGGCGTTGCC 
GCGGGAACGC CGGGCGTGCC GAACGGCGAG CGCCAGAACG GGCGCGAGAG CGAGAGGTAC 
CAGGAGGAGG AGCCTGAGCT CGCGCCGATC TGGCAGCCGC AAAGGATAGT GTGCGACATG 
CGCAATCTGG ACGAACTGGT ACGGGAGATC CTGGGTCAGT GCTCGTGTCC GTCCCAGTTC 
CCCATGACCA AGGTGTCTGA TGGGAAATAC AAAGTGGGTG ACTCCAGCGC GCTCATCTTC 
ATCAGGGTGT TGAGGACTCA TGTGATGGTG CGAGTGGGCG GAGGCTGGGA CACGCTGGAG 
CATTACCTGG ATAAACATGA CCCCTGTCGC TGCAACGCCT TCGCTCACCG GTATCAGCAG 
GGTAAGGCAG CGGGTCCGGC TCCTCACAGT AAAAGCTCCA GCGCCCACTC GTCCCGCTCT 
ACCAGCCCGG GACCCCACTG GAAGGGCGAG TCTATGGGGG CGTATAAAAG CCCAGACCGG 
CGCTCCCTGG AACCGTCACA CACCTGCCCC GCCCTGTCCT CCTCACGCCC CGGCAGAAGC 
CCCCACGTCC CGGCAGATAC AGAACCCGGG AGAAACCCGA CCGCCCTGCT GCCCAGACCC 
CCACGAGACC GCTCAGAACC ACGCCTCAAC CCCCTCAGCC TCAGGAATAA GGACTCTCTG 
CTGCCGTTGA CTCGCCGACT CTCTGGAGAC AGCGACTCCT CCAATGCCTC GTCTTGTAAA 
GGTGGCGGTG GAGCGACCGG AGGAGGTCGA CTTTCTCTGG GAGCCCGGCG CATGAATGGG 
GAGGAAGTGG TGCTGATGGT CAACCGTAAA GAGGGCAAGC ACATGATCGA GCGACCAGAG 
GGTGGAACCC ATATACCAGC CTTGCGCCCC CCTCAGACCC GGGCTCGGAG TGCTTCCAGA 
GAACGGCCTG CACCCCCATC CATGCTTAAA CCTAGCCCTC CCCAAGGTCC TGCAGAGGGG 
CACCGGGCTG CGCGGCCAGA AAGGGGCAGG TCAATGGGCC CAGAAGGTCC TAGAAGACTC 
CAAGGTCCTC GATCCCTCAG CCAGGGAAAA GCTCCTCTAC GTAGCCGAGT AAGTGAGGGC 
AGTCCTGTGT CTCCTCGCCA CAGAGATCAC AGAGACAACC GAGACCACAG AGACCTCCAA 
CCCAAAGATG AATTGAGGAG CAAGCAGGTG CCTCCAGGTT CCCCCAAGTT GAGCGGTGGC 
TTGTCCAAGC GACAAAACTC CTCTTCGGCT TCGAGTTCCC CTATCAAAGG GGGATCAGGA 
ACAAACAGCC CTGTGAAGAA GGGCATGACC CCAAGACCTC CTGCTCCACG TTCGCCTTCG 
GTGGGCAGTC GGAGGCTCCT GCCACCTGTC TCTCAGCCAG GTCGGCGCTC CCCGCACTCT 
TCGACTAACC ACCAAAAACC CCATCACCAC CCACCACGAT CCCCTCGGGT TGCCCACAGT 
CCTCGACCGC CCGGCAGGGC ACAGAGAGGA TGGAGTCAAC AACAAGCCCC CGAACCCCCG 
GAGGATGAAA TGGGAATGGG ATTTGGACTC CAGTCCCTGT CCCCTCAGGA TGCTGCACAG 
CGAGAGATGG AGCTGTACCG CACATTTGAG GCAGAGTTCC TGGCCAACAC ACAACAACAG 
CATCAGCCGG CTCAAAGCTC TAGCCATGCT AGAGCACCTA CCGCAAAGGA AGTGGGACAT 
TTGCAGCTTC CAGGCGCTGG TGACCTTAAC GTGACTGATT CAGCGTACTC TTCATCTAAC 
TCCTCTTCGT CCTCTCTAAA TGTTGGAGGG AAGATGGGAA TGTTGCCTGA TTTGCGGGAA 
TCAAAGCGGA CTCGAAATTG CGCGCTGGAG GATCCCCCTA CGCTACTGCA CAGCCAGACC 
CGAGCCGGCT TTCCCTGCAA TGGGGTGCGA GAACGAGAAC AGTGGCCCGG TCTTGGTGGG 
GGTCTCCGAA AACTCCCTGC CATTTCGAGC TCAATTGAGG AGAGCGAGCA AGTCAGAGAT 
TTTGACTCAA GACTTCCGTG TGTCCCCCGG CAGGTAAACG GAGGTCTCTC TGGTCTCCCC 
CCTGCTGAGG CACTCATGGA TTCTCAGCTG GACTACGCTG AAGTGGAGGG TGACGTTCCT 
CTGGATGATC ACCACCACCA GCCGAATCTG CCCTATGACC ACGGGGATGC CAATGATAAT 
GCTAACACTA CTAATGACCA TCCACTCCCA GAGACATTCC CTTCTCCCTT CCCACCTCCT 
CCGGATGACT GTTCCTACAA CGACTCCTCC AGCGAGAGCT CCTCCATGTG TTTTAGTTTG 
AGCGAGCCTC TTTCCGAGGG ATCCTGCACC CCTCCACCTC CTTATCCTGC CAATGGAGAT 
GTCGACAACA CAGTCGTCTT GCGCCCAAAG AAGGGGCAGA AGAAGTCAGA GAGGGTGCCT 
TCCATCTACA AGCTCAAGCT GCGTCCACGG ATACGACCTC GCACTGACAA CCGTCCGGAG 
AACAGTCCGT CACGAATTCC GACGCCGGTC AGCTACAGAG ACAGAGACCT GCACCAACGG 
CAGCAGCAGC ATTCCCCTGC GAACCTGTCC CCTCTTCAGA CGCCACCACA GTCTCCACGA 
TCCCATGGGC ATGCACCATC ACGCAAAGCT CTGCACCAGG CCTTCGCAGA CCTCATCCAC 
CCGCAGCAGA GATCACCAAC CATGGGATCA AGGGAGCGCT CGTTTTCGCT GGAGTCCAGC 
TTGGACGGGG AAGCTTGGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007228833.1
CDS	420..3683
Translation	MTTDQSNIQSSACKSIRPFKSSEEYLYAMKEDLAEWLHGLYGLDITVETFMESLETGCVLCNHANNVNRAARPFQPHRPWQDVAYQSRGAMPGSFVARDNVSNFIGWCRSELRVKDVLMFETNDLVERGSEKNFVLCLLEVARRGARFGVPAPVLVQLEQEIAREESRERERERDLKGVAAGTPGVPNGERQNGRESERYQEEEPELAPIWQPQRIVCDMRNLDELVREILGQCSCPSQFPMTKVSDGKYKVGDSSALIFIRVLRTHVMVRVGGGWDTLEHYLDKHDPCRCNAFAHRYQQGKAAGPAPHSKSSSAHSSRSTSPGPHWKGESMGAYKSPDRRSLEPSHTCPALSSSRPGRSPHVPADTEPGRNPTALLPRPPRDRSEPRLNPLSLRNKDSLLPLTRRLSGDSDSSNASSCKGGGGATGGGRLSLGARRMNGEEVVLMVNRKEGKHMIERPEGGTHIPALRPPQTRARSASRERPAPPSMLKPSPPQGPAEGHRAARPERGRSMGPEGPRRLQGPRSLSQGKAPLRSRVSEGSPVSPRHRDHRDNRDHRDLQPKDELRSKQVPPGSPKLSGGLSKRQNSSSASSSPIKGGSGTNSPVKKGMTPRPPAPRSPSVGSRRLLPPVSQPGRRSPHSSTNHQKPHHHPPRSPRVAHSPRPPGRAQRGWSQQQAPEPPEDEMGMGFGLQSLSPQDAAQREMELYRTFEAEFLANTQQQHQPAQSSSHARAPTAKEVGHLQLPGAGDLNVTDSAYSSSNSSSSSLNVGGKMGMLPDLRESKRTRNCALEDPPTLLHSQTRAGFPCNGVREREQWPGLGGGLRKLPAISSSIEESEQVRDFDSRLPCVPRQVNGGLSGLPPAEALMDSQLDYAEVEGDVPLDDHHHQPNLPYDHGDANDNANTTNDHPLPETFPSPFPPPPDDCSYNDSSSESSSMCFSLSEPLSEGSCTPPPPYPANGDVDNTVVLRPKKGQKKSERVPSIYKLKLRPRIRPRTDNRPENSPSRIPTPVSYRDRDLHQRQQQHSPANLSPLQTPPQSPRSHGHAPSRKALHQAFADLIHPQQRSPTMGSRERSFSLESSLDGEAWM

Target ORF information:

RefSeq Version	XM_007228771.2
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus growth arrest specific 2 like 1 (gas2l1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007228771.2

ORF Insert Sequence:

ATGACAACGG ACCAGAGCAA CATCCAGTCC TCTGCCTGCA AGAGCATCCG GCCGTTCAAG 
TCCAGCGAGG AGTATCTGTA CGCCATGAAG GAGGACCTGG CGGAGTGGCT CCACGGGCTC 
TACGGCCTGG ACATCACCGT GGAAACCTTC ATGGAGAGCC TGGAGACGGG CTGCGTGCTC 
TGTAACCACG CCAACAACGT CAACCGCGCC GCGCGCCCCT TCCAGCCCCA TCGCCCGTGG 
CAGGACGTGG CGTACCAGTC GCGCGGCGCC ATGCCCGGCT CCTTCGTGGC GCGCGACAAC 
GTGTCCAACT TCATCGGCTG GTGCCGCAGC GAGCTGCGCG TGAAGGACGT GCTGATGTTC 
GAGACTAACG ACCTGGTGGA GCGCGGCAGC GAGAAGAACT TCGTGCTCTG CCTCCTGGAG 
GTGGCGCGGA GAGGTGCGCG GTTCGGCGTT CCCGCTCCCG TTCTCGTACA GCTGGAGCAG 
GAGATCGCCC GCGAGGAGAG CCGGGAGCGG GAGCGGGAGA GGGACCTCAA GGGCGTTGCC 
GCGGGAACGC CGGGCGTGCC GAACGGCGAG CGCCAGAACG GGCGCGAGAG CGAGAGGTAC 
CAGGAGGAGG AGCCTGAGCT CGCGCCGATC TGGCAGCCGC AAAGGATAGT GTGCGACATG 
CGCAATCTGG ACGAACTGGT ACGGGAGATC CTGGGTCAGT GCTCGTGTCC GTCCCAGTTC 
CCCATGACCA AGGTGTCTGA TGGGAAATAC AAAGTGGGTG ACTCCAGCGC GCTCATCTTC 
ATCAGGGTGT TGAGGACTCA TGTGATGGTG CGAGTGGGCG GAGGCTGGGA CACGCTGGAG 
CATTACCTGG ATAAACATGA CCCCTGTCGC TGCAACGCCT TCGCTCACCG GTATCAGCAG 
GGTAAGGCAG CGGGTCCGGC TCCTCACAGT AAAAGCTCCA GCGCCCACTC GTCCCGCTCT 
ACCAGCCCGG GACCCCACTG GAAGGGCGAG TCTATGGGGG CGTATAAAAG CCCAGACCGG 
CGCTCCCTGG AACCGTCACA CACCTGCCCC GCCCTGTCCT CCTCACGCCC CGGCAGAAGC 
CCCCACGTCC CGGCAGATAC AGAACCCGGG AGAAACCCGA CCGCCCTGCT GCCCAGACCC 
CCACGAGACC GCTCAGAACC ACGCCTCAAC CCCCTCAGCC TCAGGAATAA GGACTCTCTG 
CTGCCGTTGA CTCGCCGACT CTCTGGAGAC AGCGACTCCT CCAATGCCTC GTCTTGTAAA 
GGTGGCGGTG GAGCGACCGG AGGAGGTCGA CTTTCTCTGG GAGCCCGGCG CATGAATGGG 
GAGGAAGTGG TGCTGATGGT CAACCGTAAA GAGGGCAAGC ACATGATCGA GCGACCAGAG 
GGTGGAACCC ATATACCAGC CTTGCGCCCC CCTCAGACCC GGGCTCGGAG TGCTTCCAGA 
GAACGGCCTG CACCCCCATC CATGCTTAAA CCTAGCCCTC CCCAAGGTCC TGCAGAGGGG 
CACCGGGCTG CGCGGCCAGA AAGGGGCAGG TCAATGGGCC CAGAAGGTCC TAGAAGACTC 
CAAGGTCCTC GATCCCTCAG CCAGGGAAAA GCTCCTCTAC GTAGCCGAGT AAGTGAGGGC 
AGTCCTGTGT CTCCTCGCCA CAGAGATCAC AGAGACAACC GAGACCACAG AGACCTCCAA 
CCCAAAGATG AATTGAGGAG CAAGCAGGTG CCTCCAGGTT CCCCCAAGTT GAGCGGTGGC 
TTGTCCAAGC GACAAAACTC CTCTTCGGCT TCGAGTTCCC CTATCAAAGG GGGATCAGGA 
ACAAACAGCC CTGTGAAGAA GGGCATGACC CCAAGACCTC CTGCTCCACG TTCGCCTTCG 
GTGGGCAGTC GGAGGCTCCT GCCACCTGTC TCTCAGCCAG GTCGGCGCTC CCCGCACTCT 
TCGACTAACC ACCAAAAACC CCATCACCAC CCACCACGAT CCCCTCGGGT TGCCCACAGT 
CCTCGACCGC CCGGCAGGGC ACAGAGAGGA TGGAGTCAAC AACAAGCCCC CGAACCCCCG 
GAGGATGAAA TGGGAATGGG ATTTGGACTC CAGTCCCTGT CCCCTCAGGA TGCTGCACAG 
CGAGAGATGG AGCTGTACCG CACATTTGAG GCAGAGTTCC TGGCCAACAC ACAACAACAG 
CATCAGCCGG CTCAAAGCTC TAGCCATGCT AGAGCACCTA CCGCAAAGGA AGTGGGACAT 
TTGCAGCTTC CAGGCGCTGG TGACCTTAAC GTGACTGATT CAGCGTACTC TTCATCTAAC 
TCCTCTTCGT CCTCTCTAAA TGTTGGAGGG AAGATGGGAA TGTTGCCTGA TTTGCGGGAA 
TCAAAGCGGA CTCGAAATTG CGCGCTGGAG GATCCCCCTA CGCTACTGCA CAGCCAGACC 
CGAGCCGGCT TTCCCTGCAA TGGGGTGCGA GAACGAGAAC AGTGGCCCGG TCTTGGTGGG 
GGTCTCCGAA AACTCCCTGC CATTTCGAGC TCAATTGAGG AGAGCGAGCA AGTCAGAGAT 
TTTGACTCAA GACTTCCGTG TGTCCCCCGG CAGGTAAACG GAGGTCTCTC TGGTCTCCCC 
CCTGCTGAGG CACTCATGGA TTCTCAGCTG GACTACGCTG AAGTGGAGGG TGACGTTCCT 
CTGGATGATC ACCACCACCA GCCGAATCTG CCCTATGACC ACGGGGATGC CAATGATAAT 
GCTAACACTA CTAATGACCA TCCACTCCCA GAGACATTCC CTTCTCCCTT CCCACCTCCT 
CCGGATGACT GTTCCTACAA CGACTCCTCC AGCGAGAGCT CCTCCATGTG TTTTAGTTTG 
AGCGAGCCTC TTTCCGAGGG ATCCTGCACC CCTCCACCTC CTTATCCTGC CAATGGAGAT 
GTCGACAACA CAGTCGTCTT GCGCCCAAAG AAGGGGCAGA AGAAGTCAGA GAGGGTGCCT 
TCCATCTACA AGCTCAAGCT GCGTCCACGG ATACGACCTC GCACTGACAA CCGTCCGGAG 
AACAGTCCGT CACGAATTCC GACGCCGGTC AGCTACAGAG ACAGAGACCT GCACCAACGG 
CAGCAGCAGC ATTCCCCTGC GAACCTGTCC CCTCTTCAGA CGCCACCACA GTCTCCACGA 
TCCCATGGGC ATGCACCATC ACGCAAAGCT CTGCACCAGG CCTTCGCAGA CCTCATCCAC 
CCGCAGCAGA GATCACCAAC CATGGGATCA AGGGAGCGCT CGTTTTCGCT GGAGTCCAGC 
TTGGACGGGG AAGCTTGGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gas2l1 ( XM_007228771.3 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007228833.2 Astyanax mexicanus growth arrest specific 2 like 1 (gas2l1), mRNA.

Price & Availability↑

CloneID	OAs99555
Clone ID Related Accession (Same CDS sequence)	XM_007228771.3
Accession Version	XM_007228771.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	GAS2-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019172819.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 3, 2017 this sequence version replaced XM_007228771.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACGG ACCAGAGCAA CATCCAGTCC TCCGCCTGCA AGAGCATCCG GCCGTTCAAG 
TCCAGCGAGG AGTATCTGTA CGCCATGAAG GAGGACCTGG CGGAGTGGCT CCACGGGCTC 
TACGGCCTGG ACATCACCGT GGAAACCTTC ATGGAGAGCC TGGAGACGGG CTGCGTGCTC 
TGTAACCACG CCAACAACGT CAACCGCGCC GCGCGCCCCT TCCAGCCCCA TCGCCCGTGG 
CAGGACGTGG CGTACCAGTC GCGCGGCGCC ATGCCCGGCT CCTTCGTGGC GCGCGACAAC 
GTGTCCAACT TCATCGGCTG GTGCCGCAGC GAGCTGCGCG TGAAGGACGT GCTGATGTTC 
GAGACTAACG ACCTGGTGGA GCGCGGCAGC GAGAAGAACT TCGTGCTCTG CCTCCTGGAG 
GTGGCGCGGA GAGGTGCGCG GTTCGGCGTT CCCGCTCCCG TTCTCGTACA GCTGGAGCAG 
GAGATCGCCC GCGAGGAGAG CCGCGAGCGG GAGCGGGAGA GGGACCTCAA GGGCGTTGCC 
GCGGGAACGC CGGGCGTGCC GAACGGCGAG CGCCAGAACG GGCGCGAGAG CGAGAGGTAC 
CAGGAGGAGG AACCTGAGCT CGCGCCGATC TGGCAGCCGC AAAGGATAGT GTGCGACATG 
CGCAATCTGG ACGAACTGGT ACGGGAGATC CTGGGTCAGT GCTCGTGTCC GTCCCAGTTC 
CCCATGACCA AGGTGTCTGA TGGGAAATAC AAAGTGGGTG ACTCCAGCGC GCTCATCTTC 
ATCAGGGTGT TGAGGACTCA TGTGATGGTG CGAGTGGGCG GAGGCTGGGA CACGCTGGAG 
CATTACCTGG ATAAACACGA CCCCTGTCGC TGCAACGCCT TCGCTCACCG GTATCAGCAG 
GGTAAGGCAG CGGGTCCGGC TCCTCACAGT AAAAGCTCCA GCGCCCACTC GTCCCGCTCT 
ACCAGCCCGG GACCCCACTG GAAGGGCGAG TCTATGGGGG CGTATAAAAG CCCAGACCGG 
CGCTCCCTGG AACCGTCACA CACCTGCCCC GCCCTGTCCT CCTCACGCCC CGGCAGAAGC 
CCCCACGTCC CGGCAGATAC AGAACCCGGG AGAAACCCGA CCACCCTGCT GCCCAGACCC 
CCACGAGACC GCTCAGAACC ACGCCTCAAC CCCCTCAGCC TCAGGAATAA GGACTCTCTG 
CTGCCGTTGA CTCGCCGACT CTCTGGAGAC AGCGACTCCT CCAATGCCTC GTCTTGTAAA 
GGTGGCGGTG GAGCGACCGG AGGAGGTCGA CTTTCTCTGG GAGCCCGGCG CATGAATGGG 
GAGGAAGTGG TGCTGATGGT CAACCGTAAA GAGGGCAAGC ACATGATCGA GCGACCAGAG 
GGTGGAACCC ATATACCAGC CTTGCGCCCC CCTCAGACCC GGGCTCGGAG TGCTTCCAGA 
GAACGGCCTG CACCCCCATC CATGCTTAAA CCTAGCCCTC CCCAAGGTCC TGCAGAGGGG 
CACCGGGCTG CGCGGCCAGA AAGGGGCAGG TCAATGGGCC CAGAAGGTCC TAGAAGACTC 
CAAGGTCCTC GATCCCTCAG CCAGGGAAAA GCTCCTCTAC GTAGCCGAGT AAGTGAGGGC 
AGTCCTGTGT CTCCTCGCCA CAGAGATCAC AGAGACAACC GAGACCACAG AGACCTCCAA 
CCCAAAGATG AATTGAGGAG CAAGCAGGTG CCTCCAGGTT CCCCCAAGTT GAGCGGTGGC 
TTGTCCAAGC GACAAAACTC CTCTTCGGCT TCGAGTTCCC CTATCAAAGG GGGATCAGGA 
ACAAACAGCC CTGTGAAGAA GGGCATGACC CCAAGACCTC CTGCTCCACG TTCGCCTTCG 
GTGGGCAGTC GGAGGCTCCT GCCACCTGTC TCTCAGCCAG GTCGGCGCTC CCCGCACTCT 
TCGACTAACC ACCAAAAACC CCATCACCAC CCACCACGAT CCCCTCGGGT TGCCCACAGT 
CCTCGACCGC CCGGCAGGGC ACAGAGAGGA TGGAGTCAAC AACAAGCCCC CGAACCCCCG 
GAGGATGAAA TGGGAATGGG ATTTGGACTC CAGTCCCTGT CCCCTCAGGA TGCTGCACAG 
CGAGAGATGG AGCTGTACCA CACATTTGAG GCAGAGTTTC TGGCCAACAC ACAACAACAG 
CATCAGCCGG CTCAAAGCTC TAGCCATGCT AGAGCACCTA CCGCAAAGGA AGTGGGACAT 
TTGCAGCTTC CAGGCGCTGG TGACCTTAAC GTGACTGATT CAGCGTACTC TTCATCTAAC 
TCCTCTTCGT CCTCTCTAAA TGTTGGAGGG AAGATGGGAA TGTTGCCTGA TTTGCGGGAA 
TCAAAGCGGA CTCGAAATTG CGCGCTGGAG GATCCCCCTA CGCTACTGCA CAGCCAGACC 
CGAGCCGGCT TTCCCTGCAA TGGGGTGCGA GAACGAGAAC AGTGGCCCGG TCTTGGTGGG 
GGTCTCCGAA AACTCCCTGC CATTTCGAGC TCAATTGAGG AGAGCGAGCA AGTCAGAGAT 
TTTGACTCAA GACTTCCGTG TGTCCCCCGG CAGGTAAACG GAGGTCTCTC TGGTCTCCCC 
CCTGCTGAGG CACTCATGGA TTCTCAGCTG GACTACGCTG TAGTGGAGGG TGACGTTCCT 
CTGGATGATC ACCACCACCA GCCGAATCTG CCCTATGACC ACGGGGATGC CAATGATAAT 
GCTAACACTA CTAATGACTA TCCACTCCCA GAGACATTCC CTTCTCCCTT CCCACCTCCT 
CCGGATGACT GTTCCTACAA CGACTCCTCC AGCGAGAGCT CCTCCATGTG TTTTAGTTTG 
AGCGAGCCTC TTTCCGAGGG AGCCTGCACC CCTCCACCTC CTTATCCTGC CAATGGAGAT 
GTCGACAACA CAGTCGTCTT GCGCCCAAAG AAGGGGCAGA AGAAGTCGGA GAGGGTGCCT 
TCCATCTACA AGCTCAAGCT GCGTCCACGG ATACGACCTC GCACTGACAA CCGTCCGGAG 
AACAGTCCGT CACGAATTCC GACGCCGGTC AGCTACAGAG ACAGAGACCT GCACCAACGG 
CAGCAGCAGC ATTCCCCTGC GAACCTGTCC CCTCTTCAGA CGCCACCACA GTCTCCACGA 
TCCCATGGGC ATGCACCATC ACGCAAAGCT CTGCACCAGG CCTTCGCAGA CCTCATCCAC 
CCGCAGCAGA GATCACCAAC CATGGGATCA AGGGAGCGCT CGTTTTCGCT GGAGTCCAGC 
TTGGACGGGG AAGCTTGGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007228833.2
CDS	442..3705
Translation	MTTDQSNIQSSACKSIRPFKSSEEYLYAMKEDLAEWLHGLYGLDITVETFMESLETGCVLCNHANNVNRAARPFQPHRPWQDVAYQSRGAMPGSFVARDNVSNFIGWCRSELRVKDVLMFETNDLVERGSEKNFVLCLLEVARRGARFGVPAPVLVQLEQEIAREESRERERERDLKGVAAGTPGVPNGERQNGRESERYQEEEPELAPIWQPQRIVCDMRNLDELVREILGQCSCPSQFPMTKVSDGKYKVGDSSALIFIRVLRTHVMVRVGGGWDTLEHYLDKHDPCRCNAFAHRYQQGKAAGPAPHSKSSSAHSSRSTSPGPHWKGESMGAYKSPDRRSLEPSHTCPALSSSRPGRSPHVPADTEPGRNPTTLLPRPPRDRSEPRLNPLSLRNKDSLLPLTRRLSGDSDSSNASSCKGGGGATGGGRLSLGARRMNGEEVVLMVNRKEGKHMIERPEGGTHIPALRPPQTRARSASRERPAPPSMLKPSPPQGPAEGHRAARPERGRSMGPEGPRRLQGPRSLSQGKAPLRSRVSEGSPVSPRHRDHRDNRDHRDLQPKDELRSKQVPPGSPKLSGGLSKRQNSSSASSSPIKGGSGTNSPVKKGMTPRPPAPRSPSVGSRRLLPPVSQPGRRSPHSSTNHQKPHHHPPRSPRVAHSPRPPGRAQRGWSQQQAPEPPEDEMGMGFGLQSLSPQDAAQREMELYHTFEAEFLANTQQQHQPAQSSSHARAPTAKEVGHLQLPGAGDLNVTDSAYSSSNSSSSSLNVGGKMGMLPDLRESKRTRNCALEDPPTLLHSQTRAGFPCNGVREREQWPGLGGGLRKLPAISSSIEESEQVRDFDSRLPCVPRQVNGGLSGLPPAEALMDSQLDYAVVEGDVPLDDHHHQPNLPYDHGDANDNANTTNDYPLPETFPSPFPPPPDDCSYNDSSSESSSMCFSLSEPLSEGACTPPPPYPANGDVDNTVVLRPKKGQKKSERVPSIYKLKLRPRIRPRTDNRPENSPSRIPTPVSYRDRDLHQRQQQHSPANLSPLQTPPQSPRSHGHAPSRKALHQAFADLIHPQQRSPTMGSRERSFSLESSLDGEAWM

Target ORF information:

RefSeq Version	XM_007228771.3
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus growth arrest specific 2 like 1 (gas2l1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007228771.3

ORF Insert Sequence:

ATGACAACGG ACCAGAGCAA CATCCAGTCC TCCGCCTGCA AGAGCATCCG GCCGTTCAAG 
TCCAGCGAGG AGTATCTGTA CGCCATGAAG GAGGACCTGG CGGAGTGGCT CCACGGGCTC 
TACGGCCTGG ACATCACCGT GGAAACCTTC ATGGAGAGCC TGGAGACGGG CTGCGTGCTC 
TGTAACCACG CCAACAACGT CAACCGCGCC GCGCGCCCCT TCCAGCCCCA TCGCCCGTGG 
CAGGACGTGG CGTACCAGTC GCGCGGCGCC ATGCCCGGCT CCTTCGTGGC GCGCGACAAC 
GTGTCCAACT TCATCGGCTG GTGCCGCAGC GAGCTGCGCG TGAAGGACGT GCTGATGTTC 
GAGACTAACG ACCTGGTGGA GCGCGGCAGC GAGAAGAACT TCGTGCTCTG CCTCCTGGAG 
GTGGCGCGGA GAGGTGCGCG GTTCGGCGTT CCCGCTCCCG TTCTCGTACA GCTGGAGCAG 
GAGATCGCCC GCGAGGAGAG CCGCGAGCGG GAGCGGGAGA GGGACCTCAA GGGCGTTGCC 
GCGGGAACGC CGGGCGTGCC GAACGGCGAG CGCCAGAACG GGCGCGAGAG CGAGAGGTAC 
CAGGAGGAGG AACCTGAGCT CGCGCCGATC TGGCAGCCGC AAAGGATAGT GTGCGACATG 
CGCAATCTGG ACGAACTGGT ACGGGAGATC CTGGGTCAGT GCTCGTGTCC GTCCCAGTTC 
CCCATGACCA AGGTGTCTGA TGGGAAATAC AAAGTGGGTG ACTCCAGCGC GCTCATCTTC 
ATCAGGGTGT TGAGGACTCA TGTGATGGTG CGAGTGGGCG GAGGCTGGGA CACGCTGGAG 
CATTACCTGG ATAAACACGA CCCCTGTCGC TGCAACGCCT TCGCTCACCG GTATCAGCAG 
GGTAAGGCAG CGGGTCCGGC TCCTCACAGT AAAAGCTCCA GCGCCCACTC GTCCCGCTCT 
ACCAGCCCGG GACCCCACTG GAAGGGCGAG TCTATGGGGG CGTATAAAAG CCCAGACCGG 
CGCTCCCTGG AACCGTCACA CACCTGCCCC GCCCTGTCCT CCTCACGCCC CGGCAGAAGC 
CCCCACGTCC CGGCAGATAC AGAACCCGGG AGAAACCCGA CCACCCTGCT GCCCAGACCC 
CCACGAGACC GCTCAGAACC ACGCCTCAAC CCCCTCAGCC TCAGGAATAA GGACTCTCTG 
CTGCCGTTGA CTCGCCGACT CTCTGGAGAC AGCGACTCCT CCAATGCCTC GTCTTGTAAA 
GGTGGCGGTG GAGCGACCGG AGGAGGTCGA CTTTCTCTGG GAGCCCGGCG CATGAATGGG 
GAGGAAGTGG TGCTGATGGT CAACCGTAAA GAGGGCAAGC ACATGATCGA GCGACCAGAG 
GGTGGAACCC ATATACCAGC CTTGCGCCCC CCTCAGACCC GGGCTCGGAG TGCTTCCAGA 
GAACGGCCTG CACCCCCATC CATGCTTAAA CCTAGCCCTC CCCAAGGTCC TGCAGAGGGG 
CACCGGGCTG CGCGGCCAGA AAGGGGCAGG TCAATGGGCC CAGAAGGTCC TAGAAGACTC 
CAAGGTCCTC GATCCCTCAG CCAGGGAAAA GCTCCTCTAC GTAGCCGAGT AAGTGAGGGC 
AGTCCTGTGT CTCCTCGCCA CAGAGATCAC AGAGACAACC GAGACCACAG AGACCTCCAA 
CCCAAAGATG AATTGAGGAG CAAGCAGGTG CCTCCAGGTT CCCCCAAGTT GAGCGGTGGC 
TTGTCCAAGC GACAAAACTC CTCTTCGGCT TCGAGTTCCC CTATCAAAGG GGGATCAGGA 
ACAAACAGCC CTGTGAAGAA GGGCATGACC CCAAGACCTC CTGCTCCACG TTCGCCTTCG 
GTGGGCAGTC GGAGGCTCCT GCCACCTGTC TCTCAGCCAG GTCGGCGCTC CCCGCACTCT 
TCGACTAACC ACCAAAAACC CCATCACCAC CCACCACGAT CCCCTCGGGT TGCCCACAGT 
CCTCGACCGC CCGGCAGGGC ACAGAGAGGA TGGAGTCAAC AACAAGCCCC CGAACCCCCG 
GAGGATGAAA TGGGAATGGG ATTTGGACTC CAGTCCCTGT CCCCTCAGGA TGCTGCACAG 
CGAGAGATGG AGCTGTACCA CACATTTGAG GCAGAGTTTC TGGCCAACAC ACAACAACAG 
CATCAGCCGG CTCAAAGCTC TAGCCATGCT AGAGCACCTA CCGCAAAGGA AGTGGGACAT 
TTGCAGCTTC CAGGCGCTGG TGACCTTAAC GTGACTGATT CAGCGTACTC TTCATCTAAC 
TCCTCTTCGT CCTCTCTAAA TGTTGGAGGG AAGATGGGAA TGTTGCCTGA TTTGCGGGAA 
TCAAAGCGGA CTCGAAATTG CGCGCTGGAG GATCCCCCTA CGCTACTGCA CAGCCAGACC 
CGAGCCGGCT TTCCCTGCAA TGGGGTGCGA GAACGAGAAC AGTGGCCCGG TCTTGGTGGG 
GGTCTCCGAA AACTCCCTGC CATTTCGAGC TCAATTGAGG AGAGCGAGCA AGTCAGAGAT 
TTTGACTCAA GACTTCCGTG TGTCCCCCGG CAGGTAAACG GAGGTCTCTC TGGTCTCCCC 
CCTGCTGAGG CACTCATGGA TTCTCAGCTG GACTACGCTG TAGTGGAGGG TGACGTTCCT 
CTGGATGATC ACCACCACCA GCCGAATCTG CCCTATGACC ACGGGGATGC CAATGATAAT 
GCTAACACTA CTAATGACTA TCCACTCCCA GAGACATTCC CTTCTCCCTT CCCACCTCCT 
CCGGATGACT GTTCCTACAA CGACTCCTCC AGCGAGAGCT CCTCCATGTG TTTTAGTTTG 
AGCGAGCCTC TTTCCGAGGG AGCCTGCACC CCTCCACCTC CTTATCCTGC CAATGGAGAT 
GTCGACAACA CAGTCGTCTT GCGCCCAAAG AAGGGGCAGA AGAAGTCGGA GAGGGTGCCT 
TCCATCTACA AGCTCAAGCT GCGTCCACGG ATACGACCTC GCACTGACAA CCGTCCGGAG 
AACAGTCCGT CACGAATTCC GACGCCGGTC AGCTACAGAG ACAGAGACCT GCACCAACGG 
CAGCAGCAGC ATTCCCCTGC GAACCTGTCC CCTCTTCAGA CGCCACCACA GTCTCCACGA 
TCCCATGGGC ATGCACCATC ACGCAAAGCT CTGCACCAGG CCTTCGCAGA CCTCATCCAC 
CCGCAGCAGA GATCACCAAC CATGGGATCA AGGGAGCGCT CGTTTTCGCT GGAGTCCAGC 
TTGGACGGGG AAGCTTGGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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gas2l1 cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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