mybpc2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mybpc2a
Entrez Gene ID	751793
Full Name	myosin binding protein C, fast type a
Synonyms	zgc:136545,zgc:136820
General protein information	Preferred Names myosin binding protein C, fast type a Names myosin-binding protein C, fast-type
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001045859.1	NP_001039324.1	myosin-binding protein C, fast-type
XM_005163952.3	XP_005164009.1	myosin-binding protein C, fast-type isoform X1
XM_021471480.1	XP_021327155.1	myosin-binding protein C, fast-type isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mybpc2	NP_001099727.1
Danio rerio (zebrafish)	mybpc2a	NP_001039324.1
Danio rerio (zebrafish)	mybpc2b	NP_001013529.2
Homo sapiens (human)	MYBPC2	NP_004524.3
Canis lupus familiaris (dog)	MYBPC2	XP_533608.2
Mus musculus (house mouse)	Mybpc2	NP_666301.2
Gallus gallus (chicken)	MYBPC2	NP_001038124.1

Gene Ontology
Bibliography

Related articles in PubMed

Ioxynil and diethylstilbestrol disrupt vascular and heart development in zebrafish.
Li YF, Canário AVM, Power DM, Campinho MA
Environment international124511-520(2019 03)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Knockdown of fast skeletal myosin-binding protein C in zebrafish results in a severe skeletal myopathy.
Li M, Andersson-Lendahl M, Sejersen T, Arner A
The Journal of general physiology147(4)309-22(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mybpc2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mybpc2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa30759	NM_001045859.1 Latest version!	Danio rerio myosin binding protein Ca (mybpc2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa08355	XM_021471480.1 Latest version!	Danio rerio myosin binding protein C, fast type a (mybpc2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa08355	XM_005163952.3 Latest version!	Danio rerio myosin binding protein C, fast type a (mybpc2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mybpc2a ( NM_001045859.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001039324.1 Danio rerio myosin binding protein Ca (mybpc2a), mRNA.

Price & Availability↑

CloneID	ODa30759
Clone ID Related Accession (Same CDS sequence)	NM_001045859.1
Accession Version	NM_001045859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576252800000
Organism	Danio rerio(zebrafish)
Product	myosin-binding protein C, fast-type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC121195.1. On Nov 21, 2012 this sequence version replaced NM_001045286.1. ##Evidence-Data-START## Transcript exon combination :: BC121195.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505391, SAMEA4476735 [ECO:0000348] ##Evidence-Data-END##

ATGCCTGAGC CGAAGAAGCC AGCTGCCAAG GAGGAAGCTC CCCCTGCTGA TAAACCTGCA 
GAAGAACATG TCCCAGGAGA AGAGTCTCAG ATGTCTGAGA TAAGTGGTCT GTTTGTGGAG 
AGGCCTCAGA CTGTCGCTGC CATTAAAGGG AAGGATGTCA CATTTGTGGC TAAGGTGGAC 
TCCTCTCAGA TGTTAAGAAA GCCAGCCGTT AAGTGGTTTA AGGGGAAATG GCTGGATCTG 
GCCAGCAAGG CTGGAAAACA CTTGCAGTTC AAAGAAACCT ATGACAGGAA CACAAAGATT 
TACACCTATG AGATGACCAT CGTTAAGGTC GTGGATGGAG ATGCTGGAGG TTATCGCTGT 
GAGGTCACGT CCAAAGACAA GTGTGACACC AGCGCATTTG ATGTTACTGT CGAAGCTGCT 
GAGGAGACAC AGCAAACAAA CATTTTGGAG GCGTTCAAGC GATCGGGAGA TGCAGGAGAG 
GATGCAGGTG ATCTGGACTT CAGTGCTTTA CTCAAGAAAA GGGAGAGGAA GCAGAAGGAG 
GAGCCTGAGG TGGATGTGGA TGTGTGGGAG ATTTTGAAAA ATGCCAAACC ATGTGATTAT 
GAGAAGATTG CCTTTGAGTA CGGCATCACT GATCTAAGAG GCATGCTTAC AAGATTGAAG 
AAGATGAAGA AGGGGGAGCC CAAAAAAAGT GATGCTTTCC TAAAGAAGTT GGATCCTGCT 
TACTCTGTGG AAAAAGGAAA GAAGATACAG CTTAGCGTGG AGCTGACTGA CCCTAATGCT 
CAAGTCAAAT GGCTGAAAAA TGGACAGGAG ATCAAACCTT CGGCCAAATA TGTCTTTGAG 
AGTGTGGGTG GCATCCGCAA GCTCACTATA AACAAGTGCA CCCTCGCTGA TGATGCCGCG 
TACGAATGTG TGGTGGGAGA GGAGAAGAGT TTCACTGAGG TCTTTATCCA AGAACCGCCT 
GTCACCATCA CTAAGATGTT GGATGATGCC CATGTGGTGG AGGGAGATAA AGTGGAGTTT 
GAGGTTGAAG TGTCTGACGA GGGAGCCAAT GTCAAATGGA TGAAGGATGG TGTGGAACTG 
AGCCGAGATG GAAAGTATCG CTTTAAGAAA GATGGGACAA AGCACTGGCT CATCATCAAT 
GAAGCAACCA CAGAGGACAT CGGGACATAC TATGTGTACA CCAGCGGAGG GGAGTCCAAG 
GCAGAGCTTG AAGTGGAAGA GAGAGAACTG GAAGTTTTGC AAAGTATAGC TGATCTGACA 
GTGAAGGCAG CAGAACAGGC CGTCTTCAAA TGTGAAGTTT CTGATGAGAA AGTGACTGGC 
AAGTGGTTCA AGGATGGGGT TGAGGTTGTC GCCAGTGACC GCATCAAAAT GTCACACATT 
GGCAGGACTC ACAAGCTGGT TATCAGTGAT GTAAAACCTG AGGACGCAGG AGATTACACG 
TTTGTGCCAG ATGGCTATGC TCTTTCTCTT TCTGCCAAAC TCAACTTCTT GGAGATCAAG 
ATTGATTACG TTCCACGTCA AGAACCACCA AAAATCCACC TGGACGCCAC CGGCAGCATG 
GTCAACAAAA ACACAATTAT TGTCGTTGCC GGAAACAAGC TCCGCTTTGA CGTTGACATC 
ACAGGCGAGC CCCCTCCCAC TGTCGCATGG AAAAAAGGAG ACATGGAGAT TTCACAAGCA 
GAAGGACGCG TGAGAGTTGA GACCAGGAAG GCATTGAGCT GTTTTGTTAT TGAAGGGGCT 
GAAAGATCTG ACGAGGGCCT CTATCATATC ACTGTAACCA ACCCAGCAGG AGAGGACAAG 
GCTGACGTTT TTGTTAAGAT AGTTGATGTG CCTGATCCAC CGGAGAATGT GAAGTGTTTG 
GGAGTGGGAG AAGATTCTGC TTCTATTGAG TGGGAGCCAC CCAAATTTGA TGGTGGTGTT 
CCAGTGAAAG GTTACCTGAT GGAGAGGAAG AAGAAGGGCT CCTCCAGGTG GACAAAGCTG 
AACTTTGACA TCTACGAGTC CACAACCTAC GAAGCTAAGA AGATGATTGA GGGTGTGTTC 
TATGAGATGA GGGTGTTTGC CGTTAATGGT ATTGGCATCT CCCAGCCCAG CGCCAATTCT 
CAACCCTTCA TGCCCATCGC TCCAACTAGT GAACCAACTC GCCTGACAGT GGACGACATT 
ACAGATGGCA CCTGTGCTCT AAAATGGCTT CCCCCTGAGA GGATTGGAGC TGGTGGTCTC 
GACGGTTACT TCATTGAATA TTGTGTCGAG GGAGCCACTG AGTGGGTGAA GGCCAACGAG 
ACACCCGTTG AGAAGAATAG TTTCAGGGTC AGAGGGCTGC CTGTTGGAGA AAAGATGCTG 
TTCAGGGTGA CAGCTGTGAA CAGGGCTGGC CTTAGCCCTT GGGCTGTGCT GGCGCAAGCT 
GTCACCATTC GGGAGATTGT TGAGAGTCCT AAAATCCGTC TCCCACGGCA TCTCAGGACT 
AAGTTCATCA GACAAGTGGG TGAGAAGGTC AACCTTGTCA TTCCTTTCCA GGGCAAACCC 
CGTCCGATGG TGACCTGGCT GAAGGATGGC GCACCAGTAG ACACTGGGCT GGTGAATATC 
CGCACAAGTG ACAAGGACAC CATCCTGTTC ATCAGGCAGT CAGCCAGAGA GCACTCTGGT 
CTGTACACCC TGTGCGTGCA GATTGAGAAC ATGAAGGACA AAGCAAATCT TGAAATCCAG 
ATTGTAGACA AGCCTGGGCC ACCAATGGCT GTCACAGTCA CTGATGTTTG GGGCTTCAAT 
GCTGCTTTGG AGTGGAAGCC TCCAAAAGAC AATGGCAACA CTGATATTAT TGGCTATACC 
GTCCAGAAGG CTGACAAGAA AACCAAGGAG TGGTTTACAG TTCTTGAACA CAATCGCAGG 
CCGAGCTGCA CTGTGTCTGA TCTGGTGATG GGGAACGAGT ACTTCTTCCG TATTTTCAGC 
GAGAACATCT GTGGCATGAG TGAGGAAGCT GGCCTCAGCA AAAACACAGC TGTCATCGCC 
AAAACAGGTC TTCAATACAA ACCCCAACCC TACAAGGAAA AAGACATGAA CAGCTCTCCG 
AAGTTTATCA CTCCTCTGGT GGACAGGTCT GTTGTGGCAG GTTACAGCGC TGCCATCAGC 
TGTGCCGTCC GTGGGTGCCC GAAACCAAAG ATTATATGGA TGAAAAATAA GATGATTATT 
GGAGAGGATC CAAAGTTTCT CATGCAGAAT AACCAGGGTG TGCTGACTTT AAACATCCGT 
AAGCCCAGCT TGTTTGATTG CGGAAAGTAT ACATGCAAAG CCATCAACGA CCTAGGAGAG 
GATGAAGTGG AGTGCAGGCT GGAGGTTCGA GTCGTAACAG AGAAGGAGGA AGCGAAGAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001039324.1
CDS	31..3393
Translation	MPEPKKPAAKEEAPPADKPAEEHVPGEESQMSEISGLFVERPQTVAAIKGKDVTFVAKVDSSQMLRKPAVKWFKGKWLDLASKAGKHLQFKETYDRNTKIYTYEMTIVKVVDGDAGGYRCEVTSKDKCDTSAFDVTVEAAEETQQTNILEAFKRSGDAGEDAGDLDFSALLKKRERKQKEEPEVDVDVWEILKNAKPCDYEKIAFEYGITDLRGMLTRLKKMKKGEPKKSDAFLKKLDPAYSVEKGKKIQLSVELTDPNAQVKWLKNGQEIKPSAKYVFESVGGIRKLTINKCTLADDAAYECVVGEEKSFTEVFIQEPPVTITKMLDDAHVVEGDKVEFEVEVSDEGANVKWMKDGVELSRDGKYRFKKDGTKHWLIINEATTEDIGTYYVYTSGGESKAELEVEERELEVLQSIADLTVKAAEQAVFKCEVSDEKVTGKWFKDGVEVVASDRIKMSHIGRTHKLVISDVKPEDAGDYTFVPDGYALSLSAKLNFLEIKIDYVPRQEPPKIHLDATGSMVNKNTIIVVAGNKLRFDVDITGEPPPTVAWKKGDMEISQAEGRVRVETRKALSCFVIEGAERSDEGLYHITVTNPAGEDKADVFVKIVDVPDPPENVKCLGVGEDSASIEWEPPKFDGGVPVKGYLMERKKKGSSRWTKLNFDIYESTTYEAKKMIEGVFYEMRVFAVNGIGISQPSANSQPFMPIAPTSEPTRLTVDDITDGTCALKWLPPERIGAGGLDGYFIEYCVEGATEWVKANETPVEKNSFRVRGLPVGEKMLFRVTAVNRAGLSPWAVLAQAVTIREIVESPKIRLPRHLRTKFIRQVGEKVNLVIPFQGKPRPMVTWLKDGAPVDTGLVNIRTSDKDTILFIRQSAREHSGLYTLCVQIENMKDKANLEIQIVDKPGPPMAVTVTDVWGFNAALEWKPPKDNGNTDIIGYTVQKADKKTKEWFTVLEHNRRPSCTVSDLVMGNEYFFRIFSENICGMSEEAGLSKNTAVIAKTGLQYKPQPYKEKDMNSSPKFITPLVDRSVVAGYSAAISCAVRGCPKPKIIWMKNKMIIGEDPKFLMQNNQGVLTLNIRKPSLFDCGKYTCKAINDLGEDEVECRLEVRVVTEKEEAKK

Target ORF information:

RefSeq Version	NM_001045859.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin binding protein Ca (mybpc2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045859.1

ORF Insert Sequence:

ATGCCTGAGC CGAAGAAGCC AGCTGCCAAG GAGGAAGCTC CCCCTGCTGA TAAACCTGCA 
GAAGAACATG TCCCAGGAGA AGAGTCTCAG ATGTCTGAGA TAAGTGGTCT GTTTGTGGAG 
AGGCCTCAGA CTGTCGCTGC CATTAAAGGG AAGGATGTCA CATTTGTGGC TAAGGTGGAC 
TCCTCTCAGA TGTTAAGAAA GCCAGCCGTT AAGTGGTTTA AGGGGAAATG GCTGGATCTG 
GCCAGCAAGG CTGGAAAACA CTTGCAGTTC AAAGAAACCT ATGACAGGAA CACAAAGATT 
TACACCTATG AGATGACCAT CGTTAAGGTC GTGGATGGAG ATGCTGGAGG TTATCGCTGT 
GAGGTCACGT CCAAAGACAA GTGTGACACC AGCGCATTTG ATGTTACTGT CGAAGCTGCT 
GAGGAGACAC AGCAAACAAA CATTTTGGAG GCGTTCAAGC GATCGGGAGA TGCAGGAGAG 
GATGCAGGTG ATCTGGACTT CAGTGCTTTA CTCAAGAAAA GGGAGAGGAA GCAGAAGGAG 
GAGCCTGAGG TGGATGTGGA TGTGTGGGAG ATTTTGAAAA ATGCCAAACC ATGTGATTAT 
GAGAAGATTG CCTTTGAGTA CGGCATCACT GATCTAAGAG GCATGCTTAC AAGATTGAAG 
AAGATGAAGA AGGGGGAGCC CAAAAAAAGT GATGCTTTCC TAAAGAAGTT GGATCCTGCT 
TACTCTGTGG AAAAAGGAAA GAAGATACAG CTTAGCGTGG AGCTGACTGA CCCTAATGCT 
CAAGTCAAAT GGCTGAAAAA TGGACAGGAG ATCAAACCTT CGGCCAAATA TGTCTTTGAG 
AGTGTGGGTG GCATCCGCAA GCTCACTATA AACAAGTGCA CCCTCGCTGA TGATGCCGCG 
TACGAATGTG TGGTGGGAGA GGAGAAGAGT TTCACTGAGG TCTTTATCCA AGAACCGCCT 
GTCACCATCA CTAAGATGTT GGATGATGCC CATGTGGTGG AGGGAGATAA AGTGGAGTTT 
GAGGTTGAAG TGTCTGACGA GGGAGCCAAT GTCAAATGGA TGAAGGATGG TGTGGAACTG 
AGCCGAGATG GAAAGTATCG CTTTAAGAAA GATGGGACAA AGCACTGGCT CATCATCAAT 
GAAGCAACCA CAGAGGACAT CGGGACATAC TATGTGTACA CCAGCGGAGG GGAGTCCAAG 
GCAGAGCTTG AAGTGGAAGA GAGAGAACTG GAAGTTTTGC AAAGTATAGC TGATCTGACA 
GTGAAGGCAG CAGAACAGGC CGTCTTCAAA TGTGAAGTTT CTGATGAGAA AGTGACTGGC 
AAGTGGTTCA AGGATGGGGT TGAGGTTGTC GCCAGTGACC GCATCAAAAT GTCACACATT 
GGCAGGACTC ACAAGCTGGT TATCAGTGAT GTAAAACCTG AGGACGCAGG AGATTACACG 
TTTGTGCCAG ATGGCTATGC TCTTTCTCTT TCTGCCAAAC TCAACTTCTT GGAGATCAAG 
ATTGATTACG TTCCACGTCA AGAACCACCA AAAATCCACC TGGACGCCAC CGGCAGCATG 
GTCAACAAAA ACACAATTAT TGTCGTTGCC GGAAACAAGC TCCGCTTTGA CGTTGACATC 
ACAGGCGAGC CCCCTCCCAC TGTCGCATGG AAAAAAGGAG ACATGGAGAT TTCACAAGCA 
GAAGGACGCG TGAGAGTTGA GACCAGGAAG GCATTGAGCT GTTTTGTTAT TGAAGGGGCT 
GAAAGATCTG ACGAGGGCCT CTATCATATC ACTGTAACCA ACCCAGCAGG AGAGGACAAG 
GCTGACGTTT TTGTTAAGAT AGTTGATGTG CCTGATCCAC CGGAGAATGT GAAGTGTTTG 
GGAGTGGGAG AAGATTCTGC TTCTATTGAG TGGGAGCCAC CCAAATTTGA TGGTGGTGTT 
CCAGTGAAAG GTTACCTGAT GGAGAGGAAG AAGAAGGGCT CCTCCAGGTG GACAAAGCTG 
AACTTTGACA TCTACGAGTC CACAACCTAC GAAGCTAAGA AGATGATTGA GGGTGTGTTC 
TATGAGATGA GGGTGTTTGC CGTTAATGGT ATTGGCATCT CCCAGCCCAG CGCCAATTCT 
CAACCCTTCA TGCCCATCGC TCCAACTAGT GAACCAACTC GCCTGACAGT GGACGACATT 
ACAGATGGCA CCTGTGCTCT AAAATGGCTT CCCCCTGAGA GGATTGGAGC TGGTGGTCTC 
GACGGTTACT TCATTGAATA TTGTGTCGAG GGAGCCACTG AGTGGGTGAA GGCCAACGAG 
ACACCCGTTG AGAAGAATAG TTTCAGGGTC AGAGGGCTGC CTGTTGGAGA AAAGATGCTG 
TTCAGGGTGA CAGCTGTGAA CAGGGCTGGC CTTAGCCCTT GGGCTGTGCT GGCGCAAGCT 
GTCACCATTC GGGAGATTGT TGAGAGTCCT AAAATCCGTC TCCCACGGCA TCTCAGGACT 
AAGTTCATCA GACAAGTGGG TGAGAAGGTC AACCTTGTCA TTCCTTTCCA GGGCAAACCC 
CGTCCGATGG TGACCTGGCT GAAGGATGGC GCACCAGTAG ACACTGGGCT GGTGAATATC 
CGCACAAGTG ACAAGGACAC CATCCTGTTC ATCAGGCAGT CAGCCAGAGA GCACTCTGGT 
CTGTACACCC TGTGCGTGCA GATTGAGAAC ATGAAGGACA AAGCAAATCT TGAAATCCAG 
ATTGTAGACA AGCCTGGGCC ACCAATGGCT GTCACAGTCA CTGATGTTTG GGGCTTCAAT 
GCTGCTTTGG AGTGGAAGCC TCCAAAAGAC AATGGCAACA CTGATATTAT TGGCTATACC 
GTCCAGAAGG CTGACAAGAA AACCAAGGAG TGGTTTACAG TTCTTGAACA CAATCGCAGG 
CCGAGCTGCA CTGTGTCTGA TCTGGTGATG GGGAACGAGT ACTTCTTCCG TATTTTCAGC 
GAGAACATCT GTGGCATGAG TGAGGAAGCT GGCCTCAGCA AAAACACAGC TGTCATCGCC 
AAAACAGGTC TTCAATACAA ACCCCAACCC TACAAGGAAA AAGACATGAA CAGCTCTCCG 
AAGTTTATCA CTCCTCTGGT GGACAGGTCT GTTGTGGCAG GTTACAGCGC TGCCATCAGC 
TGTGCCGTCC GTGGGTGCCC GAAACCAAAG ATTATATGGA TGAAAAATAA GATGATTATT 
GGAGAGGATC CAAAGTTTCT CATGCAGAAT AACCAGGGTG TGCTGACTTT AAACATCCGT 
AAGCCCAGCT TGTTTGATTG CGGAAAGTAT ACATGCAAAG CCATCAACGA CCTAGGAGAG 
GATGAAGTGG AGTGCAGGCT GGAGGTTCGA GTCGTAACAG AGAAGGAGGA AGCGAAGAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mybpc2a ( XM_005163952.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164009.1 Danio rerio myosin binding protein C, fast type a (mybpc2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08355
Clone ID Related Accession (Same CDS sequence)	XM_005163952.3 , XM_021471480.1
Accession Version	XM_005163952.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	myosin-binding protein C, fast-type isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163952.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAGC CGAAGAAGCC AGCTGCCAAG GAGGAAGCTC CCCCTGCTGA TAAACCTGCA 
GAAGATGATG ATGGCCGTGA TGAATTGCCT GAGGATGAAC ATGTCCCAGG AGAAGAGTCT 
CAGATGTCTG AGATAAGTGG TCTGTTTGTG GAGAGGCCTC AGACTGTCGC TGCCATTAAA 
GGGAAGGATG TCACATTTGT GGCTAAGGTG GACTCCTCTC AGATGTTAAG AAAGCCAGCC 
GTTAAGTGGT TTAAGGGGAA ATGGCTGGAT CTGGCCAGCA AGGCTGGAAA ACACTTGCAG 
TTCAAAGAAA CCTATGACAG GAACACAAAG ATTTACACCT ATGAGATGAC CATCGTTAAG 
GTCGTGGATG GAGATGCTGG AGGTTATCGC TGTGAGGTCA CGTCCAAAGA CAAGTGTGAC 
ACCAGCGCAT TTGATGTTAC TGTCGAAGCT GCTGAGGAGA CACAGCAAAC AAACATTTTG 
GAGGCGTTCA AGCGATCGGG AGATGCAGGA GAGGATGCAG GTGATCTGGA CTTCAGTGCT 
TTACTCAAGA AAAGGGAGAG GAAGCAGAAG GAGGAGCCTG AGGTGGATGT GGATGTGTGG 
GAGATTTTGA AAAATGCCAA ACCATGTGAT TATGAGAAGA TTGCCTTTGA GTACGGCATC 
ACTGATCTAA GAGGCATGCT TACAAGATTG AAGAAGATGA AGAAGGGGGA GCCCAAAAAA 
AGTGATGCTT TCCTAAAGAA GTTGGATCCT GCTTACTCTG TGGAAAAAGG AAAGAAGATA 
CAGCTTAGCG TGGAGCTGAC TGACCCTAAT GCTCAAGTCA AATGGCTGAA AAATGGACAG 
GAGATCAAAC CTTCGGCCAA ATATGTCTTT GAGAGTGTGG GTGGCATCCG CAAGCTCACT 
ATAAACAAGT GCACCCTCGC TGATGATGCC GCGTACGAAT GTGTGGTGGG AGAGGAGAAG 
AGTTTCACTG AGGTCTTTAT CCAAGAACCG CCTGTCACCA TCACTAAGAT GTTGGATGAT 
GCCCATGTGG TGGAGGGAGA TAAAGTGGAG TTTGAGGTTG AAGTGTCTGA CGAGGGAGCC 
AATGTCAAAT GGATGAAGGA TGGTGTGGAA CTGAGCCGAG ATGGAAAGTA TCGCTTTAAG 
AAAGATGGGA CAAAGCACTG GCTCATCATC AATGAAGCAA CCACAGAGGA CATCGGGACA 
TACTATGTGT ACACCAGCGG AGGGGAGTCC AAGGCAGAGC TTGAAGTGGA AGAGAGAGAA 
CTGGAAGTTT TGCAAAGTAT AGCTGATCTG ACAGTGAAGG CAGCAGAACA GGCCGTCTTC 
AAATGTGAAG TTTCTGATGA GAAAGTGACT GGCAAGTGGT TCAAGGATGG GGTTGAGGTT 
GTCGCCAGTG ACCGCATCAA AATGTCACAC ATTGGCAGGA CTCACAAGCT GGTTATCAGT 
GATGTAAAAC CTGAGGACGC AGGAGATTAC ACGTTTGTGC CAGATGGCTA TGCTCTTTCT 
CTTTCTGCCA AACTCAACTT CTTGGAGATC AAGATTGATT ACGTTCCACG TCAAGAACCA 
CCAAAAATCC ACCTGGACGC CACCGGCAGC ATGGTCAACA AAAACACAAT TATTGTCGTT 
GCCGGAAACA AGCTCCGCTT TGACGTTGAC ATCACAGGCG AGCCCCCTCC CACTGTCGCA 
TGGAAAAAAG GAGACATGGA GATTTCACAA GCAGAAGGAC GCGTGAGAGT TGAGACCAGG 
AAGGCATTGA GCTGTTTTGT TATTGAAGGG GCTGAAAGAT CTGACGAGGG CCTCTATCAT 
ATCACTGTAA CCAACCCAGC AGGAGAGGAC AAGGCTGACG TTTTTGTTAA GATAGTTGAT 
GTGCCTGATC CACCGGAGAA TGTGAAGTGT TTGGGAGTGG GAGAAGATTC TGCTTCTATT 
GAGTGGGAGC CACCCAAATT TGATGGTGGT GTTCCAGTGA AAGGTTACCT GATGGAGAGG 
AAGAAGAAGG GCTCCTCCAG GTGGACAAAG CTGAACTTTG ACATCTACGA GTCCACAACC 
TACGAAGCTA AGAAGATGAT TGAGGGTGTG TTCTATGAGA TGAGGGTGTT TGCCGTTAAT 
GGTATTGGCA TCTCCCAGCC CAGCGCCAAT TCTCAACCCT TCATGCCCAT CGCTCCAACT 
AGTGAACCAA CTCGCCTGAC AGTGGACGAC ATTACAGATG GCACCTGTGC TCTAAAATGG 
CTTCCCCCTG AGAGGATTGG AGCTGGTGGT CTCGACGGTT ACTTCATTGA ATATTGTGTC 
GAGGGAGCCA CTGAGTGGGT GAAGGCCAAC GAGACACCCG TTGAGAAGAA TAGTTTCAGG 
GTCAGAGGGC TGCCTGTTGG AGAAAAGATG CTGTTCAGGG TGACAGCTGT GAACAGGGCT 
GGCCTTAGCC CTTGGGCTGT GCTGGCGCAA GCTGTCACCA TTCGGGAGAT TGTTGAGAGT 
CCTAAAATCC GTCTCCCACG GCATCTCAGG ACTAAGTTCA TCAGACAAGT GGGTGAGAAG 
GTCAACCTTG TCATTCCTTT CCAGGGCAAA CCCCGTCCGA TGGTGACCTG GCTGAAGGAT 
GGCGCACCAG TAGACACTGG GCTGGTGAAT ATCCGCACAA GTGACAAGGA CACCATCCTG 
TTCATCAGGC AGTCAGCCAG AGAGCACTCT GGTCTGTACA CCCTGTGCGT GCAGATTGAG 
AACATGAAGG ACAAAGCAAA TCTTGAAATC CAGATTGTAG ACAAGCCTGG GCCACCAATG 
GCTGTCACAG TCACTGATGT TTGGGGCTTC AATGCTGCTT TGGAGTGGAA GCCTCCAAAA 
GACAATGGCA ACACTGATAT TATTGGCTAT ACCGTCCAGA AGGCTGACAA GAAAACCAAG 
GAGTGGTTTA CAGTTCTTGA ACACAATCGC AGGCCGAGCT GCACTGTGTC TGATCTGGTG 
ATGGGGAACG AGTACTTCTT CCGTATTTTC AGCGAGAACA TCTGTGGCAT GAGTGAGGAA 
GCTGGCCTCA GCAAAAACAC AGCTGTCATC GCCAAAACAG GTCTTCAATA CAAACCCCAA 
CCCTACAAGG AAAAAGACAT GAACAGCTCT CCGAAGTTTA TCACTCCTCT GGTGGACAGG 
TCTGTTGTGG CAGGTTACAG CGCTGCCATC AGCTGTGCCG TCCGTGGGTG CCCGAAACCA 
AAGATTATAT GGATGAAAAA TAAGATGATT ATTGGAGAGG ATCCAAAGTT TCTCATGCAG 
AATAACCAGG GTGTGCTGAC TTTAAACATC CGTAAGCCCA GCTTGTTTGA TTGCGGAAAG 
TATACATGCA AAGCCATCAA CGACCTAGGA GAGGATGAAG TGGAGTGCAG GCTGGAGGTT 
CGAGTCGTAA CAGAGAAGGA GGAAGCGAAG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164009.1
CDS	169..3564
Translation	MPEPKKPAAKEEAPPADKPAEDDDGRDELPEDEHVPGEESQMSEISGLFVERPQTVAAIKGKDVTFVAKVDSSQMLRKPAVKWFKGKWLDLASKAGKHLQFKETYDRNTKIYTYEMTIVKVVDGDAGGYRCEVTSKDKCDTSAFDVTVEAAEETQQTNILEAFKRSGDAGEDAGDLDFSALLKKRERKQKEEPEVDVDVWEILKNAKPCDYEKIAFEYGITDLRGMLTRLKKMKKGEPKKSDAFLKKLDPAYSVEKGKKIQLSVELTDPNAQVKWLKNGQEIKPSAKYVFESVGGIRKLTINKCTLADDAAYECVVGEEKSFTEVFIQEPPVTITKMLDDAHVVEGDKVEFEVEVSDEGANVKWMKDGVELSRDGKYRFKKDGTKHWLIINEATTEDIGTYYVYTSGGESKAELEVEERELEVLQSIADLTVKAAEQAVFKCEVSDEKVTGKWFKDGVEVVASDRIKMSHIGRTHKLVISDVKPEDAGDYTFVPDGYALSLSAKLNFLEIKIDYVPRQEPPKIHLDATGSMVNKNTIIVVAGNKLRFDVDITGEPPPTVAWKKGDMEISQAEGRVRVETRKALSCFVIEGAERSDEGLYHITVTNPAGEDKADVFVKIVDVPDPPENVKCLGVGEDSASIEWEPPKFDGGVPVKGYLMERKKKGSSRWTKLNFDIYESTTYEAKKMIEGVFYEMRVFAVNGIGISQPSANSQPFMPIAPTSEPTRLTVDDITDGTCALKWLPPERIGAGGLDGYFIEYCVEGATEWVKANETPVEKNSFRVRGLPVGEKMLFRVTAVNRAGLSPWAVLAQAVTIREIVESPKIRLPRHLRTKFIRQVGEKVNLVIPFQGKPRPMVTWLKDGAPVDTGLVNIRTSDKDTILFIRQSAREHSGLYTLCVQIENMKDKANLEIQIVDKPGPPMAVTVTDVWGFNAALEWKPPKDNGNTDIIGYTVQKADKKTKEWFTVLEHNRRPSCTVSDLVMGNEYFFRIFSENICGMSEEAGLSKNTAVIAKTGLQYKPQPYKEKDMNSSPKFITPLVDRSVVAGYSAAISCAVRGCPKPKIIWMKNKMIIGEDPKFLMQNNQGVLTLNIRKPSLFDCGKYTCKAINDLGEDEVECRLEVRVVTEKEEAKK

Target ORF information:

RefSeq Version	XM_005163952.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin binding protein C, fast type a (mybpc2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163952.3

ORF Insert Sequence:

ATGCCTGAGC CGAAGAAGCC AGCTGCCAAG GAGGAAGCTC CCCCTGCTGA TAAACCTGCA 
GAAGATGATG ATGGCCGTGA TGAATTGCCT GAGGATGAAC ATGTCCCAGG AGAAGAGTCT 
CAGATGTCTG AGATAAGTGG TCTGTTTGTG GAGAGGCCTC AGACTGTCGC TGCCATTAAA 
GGGAAGGATG TCACATTTGT GGCTAAGGTG GACTCCTCTC AGATGTTAAG AAAGCCAGCC 
GTTAAGTGGT TTAAGGGGAA ATGGCTGGAT CTGGCCAGCA AGGCTGGAAA ACACTTGCAG 
TTCAAAGAAA CCTATGACAG GAACACAAAG ATTTACACCT ATGAGATGAC CATCGTTAAG 
GTCGTGGATG GAGATGCTGG AGGTTATCGC TGTGAGGTCA CGTCCAAAGA CAAGTGTGAC 
ACCAGCGCAT TTGATGTTAC TGTCGAAGCT GCTGAGGAGA CACAGCAAAC AAACATTTTG 
GAGGCGTTCA AGCGATCGGG AGATGCAGGA GAGGATGCAG GTGATCTGGA CTTCAGTGCT 
TTACTCAAGA AAAGGGAGAG GAAGCAGAAG GAGGAGCCTG AGGTGGATGT GGATGTGTGG 
GAGATTTTGA AAAATGCCAA ACCATGTGAT TATGAGAAGA TTGCCTTTGA GTACGGCATC 
ACTGATCTAA GAGGCATGCT TACAAGATTG AAGAAGATGA AGAAGGGGGA GCCCAAAAAA 
AGTGATGCTT TCCTAAAGAA GTTGGATCCT GCTTACTCTG TGGAAAAAGG AAAGAAGATA 
CAGCTTAGCG TGGAGCTGAC TGACCCTAAT GCTCAAGTCA AATGGCTGAA AAATGGACAG 
GAGATCAAAC CTTCGGCCAA ATATGTCTTT GAGAGTGTGG GTGGCATCCG CAAGCTCACT 
ATAAACAAGT GCACCCTCGC TGATGATGCC GCGTACGAAT GTGTGGTGGG AGAGGAGAAG 
AGTTTCACTG AGGTCTTTAT CCAAGAACCG CCTGTCACCA TCACTAAGAT GTTGGATGAT 
GCCCATGTGG TGGAGGGAGA TAAAGTGGAG TTTGAGGTTG AAGTGTCTGA CGAGGGAGCC 
AATGTCAAAT GGATGAAGGA TGGTGTGGAA CTGAGCCGAG ATGGAAAGTA TCGCTTTAAG 
AAAGATGGGA CAAAGCACTG GCTCATCATC AATGAAGCAA CCACAGAGGA CATCGGGACA 
TACTATGTGT ACACCAGCGG AGGGGAGTCC AAGGCAGAGC TTGAAGTGGA AGAGAGAGAA 
CTGGAAGTTT TGCAAAGTAT AGCTGATCTG ACAGTGAAGG CAGCAGAACA GGCCGTCTTC 
AAATGTGAAG TTTCTGATGA GAAAGTGACT GGCAAGTGGT TCAAGGATGG GGTTGAGGTT 
GTCGCCAGTG ACCGCATCAA AATGTCACAC ATTGGCAGGA CTCACAAGCT GGTTATCAGT 
GATGTAAAAC CTGAGGACGC AGGAGATTAC ACGTTTGTGC CAGATGGCTA TGCTCTTTCT 
CTTTCTGCCA AACTCAACTT CTTGGAGATC AAGATTGATT ACGTTCCACG TCAAGAACCA 
CCAAAAATCC ACCTGGACGC CACCGGCAGC ATGGTCAACA AAAACACAAT TATTGTCGTT 
GCCGGAAACA AGCTCCGCTT TGACGTTGAC ATCACAGGCG AGCCCCCTCC CACTGTCGCA 
TGGAAAAAAG GAGACATGGA GATTTCACAA GCAGAAGGAC GCGTGAGAGT TGAGACCAGG 
AAGGCATTGA GCTGTTTTGT TATTGAAGGG GCTGAAAGAT CTGACGAGGG CCTCTATCAT 
ATCACTGTAA CCAACCCAGC AGGAGAGGAC AAGGCTGACG TTTTTGTTAA GATAGTTGAT 
GTGCCTGATC CACCGGAGAA TGTGAAGTGT TTGGGAGTGG GAGAAGATTC TGCTTCTATT 
GAGTGGGAGC CACCCAAATT TGATGGTGGT GTTCCAGTGA AAGGTTACCT GATGGAGAGG 
AAGAAGAAGG GCTCCTCCAG GTGGACAAAG CTGAACTTTG ACATCTACGA GTCCACAACC 
TACGAAGCTA AGAAGATGAT TGAGGGTGTG TTCTATGAGA TGAGGGTGTT TGCCGTTAAT 
GGTATTGGCA TCTCCCAGCC CAGCGCCAAT TCTCAACCCT TCATGCCCAT CGCTCCAACT 
AGTGAACCAA CTCGCCTGAC AGTGGACGAC ATTACAGATG GCACCTGTGC TCTAAAATGG 
CTTCCCCCTG AGAGGATTGG AGCTGGTGGT CTCGACGGTT ACTTCATTGA ATATTGTGTC 
GAGGGAGCCA CTGAGTGGGT GAAGGCCAAC GAGACACCCG TTGAGAAGAA TAGTTTCAGG 
GTCAGAGGGC TGCCTGTTGG AGAAAAGATG CTGTTCAGGG TGACAGCTGT GAACAGGGCT 
GGCCTTAGCC CTTGGGCTGT GCTGGCGCAA GCTGTCACCA TTCGGGAGAT TGTTGAGAGT 
CCTAAAATCC GTCTCCCACG GCATCTCAGG ACTAAGTTCA TCAGACAAGT GGGTGAGAAG 
GTCAACCTTG TCATTCCTTT CCAGGGCAAA CCCCGTCCGA TGGTGACCTG GCTGAAGGAT 
GGCGCACCAG TAGACACTGG GCTGGTGAAT ATCCGCACAA GTGACAAGGA CACCATCCTG 
TTCATCAGGC AGTCAGCCAG AGAGCACTCT GGTCTGTACA CCCTGTGCGT GCAGATTGAG 
AACATGAAGG ACAAAGCAAA TCTTGAAATC CAGATTGTAG ACAAGCCTGG GCCACCAATG 
GCTGTCACAG TCACTGATGT TTGGGGCTTC AATGCTGCTT TGGAGTGGAA GCCTCCAAAA 
GACAATGGCA ACACTGATAT TATTGGCTAT ACCGTCCAGA AGGCTGACAA GAAAACCAAG 
GAGTGGTTTA CAGTTCTTGA ACACAATCGC AGGCCGAGCT GCACTGTGTC TGATCTGGTG 
ATGGGGAACG AGTACTTCTT CCGTATTTTC AGCGAGAACA TCTGTGGCAT GAGTGAGGAA 
GCTGGCCTCA GCAAAAACAC AGCTGTCATC GCCAAAACAG GTCTTCAATA CAAACCCCAA 
CCCTACAAGG AAAAAGACAT GAACAGCTCT CCGAAGTTTA TCACTCCTCT GGTGGACAGG 
TCTGTTGTGG CAGGTTACAG CGCTGCCATC AGCTGTGCCG TCCGTGGGTG CCCGAAACCA 
AAGATTATAT GGATGAAAAA TAAGATGATT ATTGGAGAGG ATCCAAAGTT TCTCATGCAG 
AATAACCAGG GTGTGCTGAC TTTAAACATC CGTAAGCCCA GCTTGTTTGA TTGCGGAAAG 
TATACATGCA AAGCCATCAA CGACCTAGGA GAGGATGAAG TGGAGTGCAG GCTGGAGGTT 
CGAGTCGTAA CAGAGAAGGA GGAAGCGAAG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mybpc2a ( XM_021471480.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327155.1 Danio rerio myosin binding protein C, fast type a (mybpc2a), transcript variant X1, mRNA.

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CloneID	ODa08355
Clone ID Related Accession (Same CDS sequence)	XM_005163952.3 , XM_021471480.1
Accession Version	XM_021471480.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	myosin-binding protein C, fast-type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAGC CGAAGAAGCC AGCTGCCAAG GAGGAAGCTC CCCCTGCTGA TAAACCTGCA 
GAAGATGATG ATGGCCGTGA TGAATTGCCT GAGGATGAAC ATGTCCCAGG AGAAGAGTCT 
CAGATGTCTG AGATAAGTGG TCTGTTTGTG GAGAGGCCTC AGACTGTCGC TGCCATTAAA 
GGGAAGGATG TCACATTTGT GGCTAAGGTG GACTCCTCTC AGATGTTAAG AAAGCCAGCC 
GTTAAGTGGT TTAAGGGGAA ATGGCTGGAT CTGGCCAGCA AGGCTGGAAA ACACTTGCAG 
TTCAAAGAAA CCTATGACAG GAACACAAAG ATTTACACCT ATGAGATGAC CATCGTTAAG 
GTCGTGGATG GAGATGCTGG AGGTTATCGC TGTGAGGTCA CGTCCAAAGA CAAGTGTGAC 
ACCAGCGCAT TTGATGTTAC TGTCGAAGCT GCTGAGGAGA CACAGCAAAC AAACATTTTG 
GAGGCGTTCA AGCGATCGGG AGATGCAGGA GAGGATGCAG GTGATCTGGA CTTCAGTGCT 
TTACTCAAGA AAAGGGAGAG GAAGCAGAAG GAGGAGCCTG AGGTGGATGT GGATGTGTGG 
GAGATTTTGA AAAATGCCAA ACCATGTGAT TATGAGAAGA TTGCCTTTGA GTACGGCATC 
ACTGATCTAA GAGGCATGCT TACAAGATTG AAGAAGATGA AGAAGGGGGA GCCCAAAAAA 
AGTGATGCTT TCCTAAAGAA GTTGGATCCT GCTTACTCTG TGGAAAAAGG AAAGAAGATA 
CAGCTTAGCG TGGAGCTGAC TGACCCTAAT GCTCAAGTCA AATGGCTGAA AAATGGACAG 
GAGATCAAAC CTTCGGCCAA ATATGTCTTT GAGAGTGTGG GTGGCATCCG CAAGCTCACT 
ATAAACAAGT GCACCCTCGC TGATGATGCC GCGTACGAAT GTGTGGTGGG AGAGGAGAAG 
AGTTTCACTG AGGTCTTTAT CCAAGAACCG CCTGTCACCA TCACTAAGAT GTTGGATGAT 
GCCCATGTGG TGGAGGGAGA TAAAGTGGAG TTTGAGGTTG AAGTGTCTGA CGAGGGAGCC 
AATGTCAAAT GGATGAAGGA TGGTGTGGAA CTGAGCCGAG ATGGAAAGTA TCGCTTTAAG 
AAAGATGGGA CAAAGCACTG GCTCATCATC AATGAAGCAA CCACAGAGGA CATCGGGACA 
TACTATGTGT ACACCAGCGG AGGGGAGTCC AAGGCAGAGC TTGAAGTGGA AGAGAGAGAA 
CTGGAAGTTT TGCAAAGTAT AGCTGATCTG ACAGTGAAGG CAGCAGAACA GGCCGTCTTC 
AAATGTGAAG TTTCTGATGA GAAAGTGACT GGCAAGTGGT TCAAGGATGG GGTTGAGGTT 
GTCGCCAGTG ACCGCATCAA AATGTCACAC ATTGGCAGGA CTCACAAGCT GGTTATCAGT 
GATGTAAAAC CTGAGGACGC AGGAGATTAC ACGTTTGTGC CAGATGGCTA TGCTCTTTCT 
CTTTCTGCCA AACTCAACTT CTTGGAGATC AAGATTGATT ACGTTCCACG TCAAGAACCA 
CCAAAAATCC ACCTGGACGC CACCGGCAGC ATGGTCAACA AAAACACAAT TATTGTCGTT 
GCCGGAAACA AGCTCCGCTT TGACGTTGAC ATCACAGGCG AGCCCCCTCC CACTGTCGCA 
TGGAAAAAAG GAGACATGGA GATTTCACAA GCAGAAGGAC GCGTGAGAGT TGAGACCAGG 
AAGGCATTGA GCTGTTTTGT TATTGAAGGG GCTGAAAGAT CTGACGAGGG CCTCTATCAT 
ATCACTGTAA CCAACCCAGC AGGAGAGGAC AAGGCTGACG TTTTTGTTAA GATAGTTGAT 
GTGCCTGATC CACCGGAGAA TGTGAAGTGT TTGGGAGTGG GAGAAGATTC TGCTTCTATT 
GAGTGGGAGC CACCCAAATT TGATGGTGGT GTTCCAGTGA AAGGTTACCT GATGGAGAGG 
AAGAAGAAGG GCTCCTCCAG GTGGACAAAG CTGAACTTTG ACATCTACGA GTCCACAACC 
TACGAAGCTA AGAAGATGAT TGAGGGTGTG TTCTATGAGA TGAGGGTGTT TGCCGTTAAT 
GGTATTGGCA TCTCCCAGCC CAGCGCCAAT TCTCAACCCT TCATGCCCAT CGCTCCAACT 
AGTGAACCAA CTCGCCTGAC AGTGGACGAC ATTACAGATG GCACCTGTGC TCTAAAATGG 
CTTCCCCCTG AGAGGATTGG AGCTGGTGGT CTCGACGGTT ACTTCATTGA ATATTGTGTC 
GAGGGAGCCA CTGAGTGGGT GAAGGCCAAC GAGACACCCG TTGAGAAGAA TAGTTTCAGG 
GTCAGAGGGC TGCCTGTTGG AGAAAAGATG CTGTTCAGGG TGACAGCTGT GAACAGGGCT 
GGCCTTAGCC CTTGGGCTGT GCTGGCGCAA GCTGTCACCA TTCGGGAGAT TGTTGAGAGT 
CCTAAAATCC GTCTCCCACG GCATCTCAGG ACTAAGTTCA TCAGACAAGT GGGTGAGAAG 
GTCAACCTTG TCATTCCTTT CCAGGGCAAA CCCCGTCCGA TGGTGACCTG GCTGAAGGAT 
GGCGCACCAG TAGACACTGG GCTGGTGAAT ATCCGCACAA GTGACAAGGA CACCATCCTG 
TTCATCAGGC AGTCAGCCAG AGAGCACTCT GGTCTGTACA CCCTGTGCGT GCAGATTGAG 
AACATGAAGG ACAAAGCAAA TCTTGAAATC CAGATTGTAG ACAAGCCTGG GCCACCAATG 
GCTGTCACAG TCACTGATGT TTGGGGCTTC AATGCTGCTT TGGAGTGGAA GCCTCCAAAA 
GACAATGGCA ACACTGATAT TATTGGCTAT ACCGTCCAGA AGGCTGACAA GAAAACCAAG 
GAGTGGTTTA CAGTTCTTGA ACACAATCGC AGGCCGAGCT GCACTGTGTC TGATCTGGTG 
ATGGGGAACG AGTACTTCTT CCGTATTTTC AGCGAGAACA TCTGTGGCAT GAGTGAGGAA 
GCTGGCCTCA GCAAAAACAC AGCTGTCATC GCCAAAACAG GTCTTCAATA CAAACCCCAA 
CCCTACAAGG AAAAAGACAT GAACAGCTCT CCGAAGTTTA TCACTCCTCT GGTGGACAGG 
TCTGTTGTGG CAGGTTACAG CGCTGCCATC AGCTGTGCCG TCCGTGGGTG CCCGAAACCA 
AAGATTATAT GGATGAAAAA TAAGATGATT ATTGGAGAGG ATCCAAAGTT TCTCATGCAG 
AATAACCAGG GTGTGCTGAC TTTAAACATC CGTAAGCCCA GCTTGTTTGA TTGCGGAAAG 
TATACATGCA AAGCCATCAA CGACCTAGGA GAGGATGAAG TGGAGTGCAG GCTGGAGGTT 
CGAGTCGTAA CAGAGAAGGA GGAAGCGAAG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327155.1
CDS	102..3497
Translation	MPEPKKPAAKEEAPPADKPAEDDDGRDELPEDEHVPGEESQMSEISGLFVERPQTVAAIKGKDVTFVAKVDSSQMLRKPAVKWFKGKWLDLASKAGKHLQFKETYDRNTKIYTYEMTIVKVVDGDAGGYRCEVTSKDKCDTSAFDVTVEAAEETQQTNILEAFKRSGDAGEDAGDLDFSALLKKRERKQKEEPEVDVDVWEILKNAKPCDYEKIAFEYGITDLRGMLTRLKKMKKGEPKKSDAFLKKLDPAYSVEKGKKIQLSVELTDPNAQVKWLKNGQEIKPSAKYVFESVGGIRKLTINKCTLADDAAYECVVGEEKSFTEVFIQEPPVTITKMLDDAHVVEGDKVEFEVEVSDEGANVKWMKDGVELSRDGKYRFKKDGTKHWLIINEATTEDIGTYYVYTSGGESKAELEVEERELEVLQSIADLTVKAAEQAVFKCEVSDEKVTGKWFKDGVEVVASDRIKMSHIGRTHKLVISDVKPEDAGDYTFVPDGYALSLSAKLNFLEIKIDYVPRQEPPKIHLDATGSMVNKNTIIVVAGNKLRFDVDITGEPPPTVAWKKGDMEISQAEGRVRVETRKALSCFVIEGAERSDEGLYHITVTNPAGEDKADVFVKIVDVPDPPENVKCLGVGEDSASIEWEPPKFDGGVPVKGYLMERKKKGSSRWTKLNFDIYESTTYEAKKMIEGVFYEMRVFAVNGIGISQPSANSQPFMPIAPTSEPTRLTVDDITDGTCALKWLPPERIGAGGLDGYFIEYCVEGATEWVKANETPVEKNSFRVRGLPVGEKMLFRVTAVNRAGLSPWAVLAQAVTIREIVESPKIRLPRHLRTKFIRQVGEKVNLVIPFQGKPRPMVTWLKDGAPVDTGLVNIRTSDKDTILFIRQSAREHSGLYTLCVQIENMKDKANLEIQIVDKPGPPMAVTVTDVWGFNAALEWKPPKDNGNTDIIGYTVQKADKKTKEWFTVLEHNRRPSCTVSDLVMGNEYFFRIFSENICGMSEEAGLSKNTAVIAKTGLQYKPQPYKEKDMNSSPKFITPLVDRSVVAGYSAAISCAVRGCPKPKIIWMKNKMIIGEDPKFLMQNNQGVLTLNIRKPSLFDCGKYTCKAINDLGEDEVECRLEVRVVTEKEEAKK

Target ORF information:

RefSeq Version	XM_021471480.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin binding protein C, fast type a (mybpc2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471480.1

ORF Insert Sequence:

ATGCCTGAGC CGAAGAAGCC AGCTGCCAAG GAGGAAGCTC CCCCTGCTGA TAAACCTGCA 
GAAGATGATG ATGGCCGTGA TGAATTGCCT GAGGATGAAC ATGTCCCAGG AGAAGAGTCT 
CAGATGTCTG AGATAAGTGG TCTGTTTGTG GAGAGGCCTC AGACTGTCGC TGCCATTAAA 
GGGAAGGATG TCACATTTGT GGCTAAGGTG GACTCCTCTC AGATGTTAAG AAAGCCAGCC 
GTTAAGTGGT TTAAGGGGAA ATGGCTGGAT CTGGCCAGCA AGGCTGGAAA ACACTTGCAG 
TTCAAAGAAA CCTATGACAG GAACACAAAG ATTTACACCT ATGAGATGAC CATCGTTAAG 
GTCGTGGATG GAGATGCTGG AGGTTATCGC TGTGAGGTCA CGTCCAAAGA CAAGTGTGAC 
ACCAGCGCAT TTGATGTTAC TGTCGAAGCT GCTGAGGAGA CACAGCAAAC AAACATTTTG 
GAGGCGTTCA AGCGATCGGG AGATGCAGGA GAGGATGCAG GTGATCTGGA CTTCAGTGCT 
TTACTCAAGA AAAGGGAGAG GAAGCAGAAG GAGGAGCCTG AGGTGGATGT GGATGTGTGG 
GAGATTTTGA AAAATGCCAA ACCATGTGAT TATGAGAAGA TTGCCTTTGA GTACGGCATC 
ACTGATCTAA GAGGCATGCT TACAAGATTG AAGAAGATGA AGAAGGGGGA GCCCAAAAAA 
AGTGATGCTT TCCTAAAGAA GTTGGATCCT GCTTACTCTG TGGAAAAAGG AAAGAAGATA 
CAGCTTAGCG TGGAGCTGAC TGACCCTAAT GCTCAAGTCA AATGGCTGAA AAATGGACAG 
GAGATCAAAC CTTCGGCCAA ATATGTCTTT GAGAGTGTGG GTGGCATCCG CAAGCTCACT 
ATAAACAAGT GCACCCTCGC TGATGATGCC GCGTACGAAT GTGTGGTGGG AGAGGAGAAG 
AGTTTCACTG AGGTCTTTAT CCAAGAACCG CCTGTCACCA TCACTAAGAT GTTGGATGAT 
GCCCATGTGG TGGAGGGAGA TAAAGTGGAG TTTGAGGTTG AAGTGTCTGA CGAGGGAGCC 
AATGTCAAAT GGATGAAGGA TGGTGTGGAA CTGAGCCGAG ATGGAAAGTA TCGCTTTAAG 
AAAGATGGGA CAAAGCACTG GCTCATCATC AATGAAGCAA CCACAGAGGA CATCGGGACA 
TACTATGTGT ACACCAGCGG AGGGGAGTCC AAGGCAGAGC TTGAAGTGGA AGAGAGAGAA 
CTGGAAGTTT TGCAAAGTAT AGCTGATCTG ACAGTGAAGG CAGCAGAACA GGCCGTCTTC 
AAATGTGAAG TTTCTGATGA GAAAGTGACT GGCAAGTGGT TCAAGGATGG GGTTGAGGTT 
GTCGCCAGTG ACCGCATCAA AATGTCACAC ATTGGCAGGA CTCACAAGCT GGTTATCAGT 
GATGTAAAAC CTGAGGACGC AGGAGATTAC ACGTTTGTGC CAGATGGCTA TGCTCTTTCT 
CTTTCTGCCA AACTCAACTT CTTGGAGATC AAGATTGATT ACGTTCCACG TCAAGAACCA 
CCAAAAATCC ACCTGGACGC CACCGGCAGC ATGGTCAACA AAAACACAAT TATTGTCGTT 
GCCGGAAACA AGCTCCGCTT TGACGTTGAC ATCACAGGCG AGCCCCCTCC CACTGTCGCA 
TGGAAAAAAG GAGACATGGA GATTTCACAA GCAGAAGGAC GCGTGAGAGT TGAGACCAGG 
AAGGCATTGA GCTGTTTTGT TATTGAAGGG GCTGAAAGAT CTGACGAGGG CCTCTATCAT 
ATCACTGTAA CCAACCCAGC AGGAGAGGAC AAGGCTGACG TTTTTGTTAA GATAGTTGAT 
GTGCCTGATC CACCGGAGAA TGTGAAGTGT TTGGGAGTGG GAGAAGATTC TGCTTCTATT 
GAGTGGGAGC CACCCAAATT TGATGGTGGT GTTCCAGTGA AAGGTTACCT GATGGAGAGG 
AAGAAGAAGG GCTCCTCCAG GTGGACAAAG CTGAACTTTG ACATCTACGA GTCCACAACC 
TACGAAGCTA AGAAGATGAT TGAGGGTGTG TTCTATGAGA TGAGGGTGTT TGCCGTTAAT 
GGTATTGGCA TCTCCCAGCC CAGCGCCAAT TCTCAACCCT TCATGCCCAT CGCTCCAACT 
AGTGAACCAA CTCGCCTGAC AGTGGACGAC ATTACAGATG GCACCTGTGC TCTAAAATGG 
CTTCCCCCTG AGAGGATTGG AGCTGGTGGT CTCGACGGTT ACTTCATTGA ATATTGTGTC 
GAGGGAGCCA CTGAGTGGGT GAAGGCCAAC GAGACACCCG TTGAGAAGAA TAGTTTCAGG 
GTCAGAGGGC TGCCTGTTGG AGAAAAGATG CTGTTCAGGG TGACAGCTGT GAACAGGGCT 
GGCCTTAGCC CTTGGGCTGT GCTGGCGCAA GCTGTCACCA TTCGGGAGAT TGTTGAGAGT 
CCTAAAATCC GTCTCCCACG GCATCTCAGG ACTAAGTTCA TCAGACAAGT GGGTGAGAAG 
GTCAACCTTG TCATTCCTTT CCAGGGCAAA CCCCGTCCGA TGGTGACCTG GCTGAAGGAT 
GGCGCACCAG TAGACACTGG GCTGGTGAAT ATCCGCACAA GTGACAAGGA CACCATCCTG 
TTCATCAGGC AGTCAGCCAG AGAGCACTCT GGTCTGTACA CCCTGTGCGT GCAGATTGAG 
AACATGAAGG ACAAAGCAAA TCTTGAAATC CAGATTGTAG ACAAGCCTGG GCCACCAATG 
GCTGTCACAG TCACTGATGT TTGGGGCTTC AATGCTGCTT TGGAGTGGAA GCCTCCAAAA 
GACAATGGCA ACACTGATAT TATTGGCTAT ACCGTCCAGA AGGCTGACAA GAAAACCAAG 
GAGTGGTTTA CAGTTCTTGA ACACAATCGC AGGCCGAGCT GCACTGTGTC TGATCTGGTG 
ATGGGGAACG AGTACTTCTT CCGTATTTTC AGCGAGAACA TCTGTGGCAT GAGTGAGGAA 
GCTGGCCTCA GCAAAAACAC AGCTGTCATC GCCAAAACAG GTCTTCAATA CAAACCCCAA 
CCCTACAAGG AAAAAGACAT GAACAGCTCT CCGAAGTTTA TCACTCCTCT GGTGGACAGG 
TCTGTTGTGG CAGGTTACAG CGCTGCCATC AGCTGTGCCG TCCGTGGGTG CCCGAAACCA 
AAGATTATAT GGATGAAAAA TAAGATGATT ATTGGAGAGG ATCCAAAGTT TCTCATGCAG 
AATAACCAGG GTGTGCTGAC TTTAAACATC CGTAAGCCCA GCTTGTTTGA TTGCGGAAAG 
TATACATGCA AAGCCATCAA CGACCTAGGA GAGGATGAAG TGGAGTGCAG GCTGGAGGTT 
CGAGTCGTAA CAGAGAAGGA GGAAGCGAAG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ioxynil and diethylstilbestrol disrupt vascular and heart development in zebrafish.
Environment international124511-520(2019 03)
Li YF,Canário AVM,Power DM,Campinho MA

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Knockdown of fast skeletal myosin-binding protein C in zebrafish results in a severe skeletal myopathy.
The Journal of general physiology147(4)309-22(2016 Apr)
Li M,Andersson-Lendahl M,Sejersen T,Arner A

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mybpc2a cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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