myf5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	myf5
Entrez Gene ID	58097
Full Name	myogenic factor 5
Synonyms	cb641,myf-5,zgc:110509
General protein information	Preferred Names myogenic factor 5 Names myogenic factor 5 myogenic regulatory factor 5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131576.1	NP_571651.1	myogenic factor 5

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MYF5	XP_852162.1
Bos taurus (cattle)	MYF5	NP_776541.1
Danio rerio (zebrafish)	myf5	NP_571651.1
Pan troglodytes (chimpanzee)	MYF5	XP_522481.2
Macaca mulatta (Rhesus monkey)	MYF5	XP_001087187.1
Rattus norvegicus (Norway rat)	Myf5	NP_001100253.1
Homo sapiens (human)	MYF5	NP_005584.2
Mus musculus (house mouse)	Myf5	NP_032682.1
Gallus gallus (chicken)	MYF5	NP_001025534.1
Xenopus tropicalis (tropical clawed frog)	myf5	NP_988932.1

Gene Ontology
Bibliography

Related articles in PubMed

Fer1l6 is essential for the development of vertebrate muscle tissue in zebrafish.
Bonventre JA, Holman C, Manchanda A, Codding SJ, Chau T, Huegel J, Barton C, Tanguay R, Johnson CP
Molecular biology of the cell30(3)293-301(2019 02)

Environmentally relevant concentrations of the flame retardant tris(1,3-dichloro-2-propyl) phosphate change morphology of female zebrafish.
Zhu Y, Lin D, Yang D, Jia Y, Liu C
Chemosphere212358-364(2018 Dec)

Identification of Dmrt2a downstream genes during zebrafish early development using a timely controlled approach.
Pinto RA, Almeida-Santos J, Lourenço R, Saúde L
BMC developmental biology18(1)14(2018 06)

BMP and FGF signaling interact to pattern mesoderm by controlling basic helix-loop-helix transcription factor activity.
Row RH, Pegg A, Kinney BA, Farr GH 3rd, Maves L, Lowell S, Wilson V, Martin BL
eLife7(2018 06)

The DEAD-box RNA helicase Ddx39ab is essential for myocyte and lens development in zebrafish.
Zhang L, Yang Y, Li B, Scott IC, Lou X
Development (Cambridge, England)145(8)(2018 04)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following myf5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myf5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00471	NM_131576.1 Latest version!	Danio rerio myogenic factor 5 (myf5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

myf5 ( NM_131576.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571651.1 Danio rerio myogenic factor 5 (myf5), mRNA.

Price & Availability↑

CloneID	ODa00471
Clone ID Related Accession (Same CDS sequence)	NM_131576.1
Accession Version	NM_131576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1561132800000
Organism	Danio rerio(zebrafish)
Product	myogenic factor 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF270789.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF270789.1, CT659946.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END##

ATGGACGTAT TCTCCACATC CCAGATCTTC TACGACAGCA CTTGCGCTTC GTCTCCTGAA 
GATTTAGAGT TTGGAGCCAG TGGGGAACTG ACCGGGTCTG AGGAGGATGA GCACGTGCGG 
GCTCCTGGGG CCCCACATCA ACCGGGCCAT TGTCTCCAAT GGGCCTGCAA AGCTTGCAAG 
CGTAAAGCCA GTACGGTGGA CCGCCGGAGA GCCGCCACCA TGAGGGAACG GCGCAGGCTG 
AAGAAGGTCA ATCACGCCTT TGAGGCACTA CGCCGCTGCA CCTCGGCCAA CCCTAGCCAA 
CGCCTCCCCA AGGTAGAGAT CCTGAGGAAC GCCATCCAGT ACATCGAGAG CCTTCAGGAG 
CTCCTCAGGG AACAAGTGGA GCAATACTAC AGCCTGCCGA TGGAGAGCAG CTCTGAGCCC 
GCCAGTCCCT CCTCCAGCTG CTCAGAGAGC ATGGTTGACT GCAACAGTCC TGTATGGCCT 
CAGATGAATC AAAACTATGG GAATAGCTAC AACTTTGACG CACAAAATGC TAGCACAATG 
GAGCGAACTC CAGGAGTGTC CAGTTTGCAG TGTTTGTCCA GCATCGTGGA CAGACTGTCC 
TCTGTAGATC CTGCGGGAAT GAGGAACATG GTCGTTCTTT CTCCAACCGG AAGTGATTCC 
CAGTCCAGTT CTCCAGACAG TCCAAACAAC AGACCAGTTT ACCACGTACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571651.1
CDS	44..757
Translation	MDVFSTSQIFYDSTCASSPEDLEFGASGELTGSEEDEHVRAPGAPHQPGHCLQWACKACKRKASTVDRRRAATMRERRRLKKVNHAFEALRRCTSANPSQRLPKVEILRNAIQYIESLQELLREQVEQYYSLPMESSSEPASPSSSCSESMVDCNSPVWPQMNQNYGNSYNFDAQNASTMERTPGVSSLQCLSSIVDRLSSVDPAGMRNMVVLSPTGSDSQSSSPDSPNNRPVYHVL

Target ORF information:

RefSeq Version	NM_131576.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myogenic factor 5 (myf5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131576.1

ORF Insert Sequence:

ATGGACGTAT TCTCCACATC CCAGATCTTC TACGACAGCA CTTGCGCTTC GTCTCCTGAA 
GATTTAGAGT TTGGAGCCAG TGGGGAACTG ACCGGGTCTG AGGAGGATGA GCACGTGCGG 
GCTCCTGGGG CCCCACATCA ACCGGGCCAT TGTCTCCAAT GGGCCTGCAA AGCTTGCAAG 
CGTAAAGCCA GTACGGTGGA CCGCCGGAGA GCCGCCACCA TGAGGGAACG GCGCAGGCTG 
AAGAAGGTCA ATCACGCCTT TGAGGCACTA CGCCGCTGCA CCTCGGCCAA CCCTAGCCAA 
CGCCTCCCCA AGGTAGAGAT CCTGAGGAAC GCCATCCAGT ACATCGAGAG CCTTCAGGAG 
CTCCTCAGGG AACAAGTGGA GCAATACTAC AGCCTGCCGA TGGAGAGCAG CTCTGAGCCC 
GCCAGTCCCT CCTCCAGCTG CTCAGAGAGC ATGGTTGACT GCAACAGTCC TGTATGGCCT 
CAGATGAATC AAAACTATGG GAATAGCTAC AACTTTGACG CACAAAATGC TAGCACAATG 
GAGCGAACTC CAGGAGTGTC CAGTTTGCAG TGTTTGTCCA GCATCGTGGA CAGACTGTCC 
TCTGTAGATC CTGCGGGAAT GAGGAACATG GTCGTTCTTT CTCCAACCGG AAGTGATTCC 
CAGTCCAGTT CTCCAGACAG TCCAAACAAC AGACCAGTTT ACCACGTACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Fer1l6 is essential for the development of vertebrate muscle tissue in zebrafish.
Molecular biology of the cell30(3)293-301(2019 02)
Bonventre JA,Holman C,Manchanda A,Codding SJ,Chau T,Huegel J,Barton C,Tanguay R,Johnson CP

Environmentally relevant concentrations of the flame retardant tris(1,3-dichloro-2-propyl) phosphate change morphology of female zebrafish.
Chemosphere212358-364(2018 Dec)
Zhu Y,Lin D,Yang D,Jia Y,Liu C

Identification of Dmrt2a downstream genes during zebrafish early development using a timely controlled approach.
BMC developmental biology18(1)14(2018 06)
Pinto RA,Almeida-Santos J,Lourenço R,Saúde L

BMP and FGF signaling interact to pattern mesoderm by controlling basic helix-loop-helix transcription factor activity.
eLife7(2018 06)
Row RH,Pegg A,Kinney BA,Farr GH 3rd,Maves L,Lowell S,Wilson V,Martin BL

The DEAD-box RNA helicase Ddx39ab is essential for myocyte and lens development in zebrafish.
Development (Cambridge, England)145(8)(2018 04)
Zhang L,Yang Y,Li B,Scott IC,Lou X