SI:CH211-11P18.6 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI:CH211-11P18.6
Entrez Gene ID	566957
Full Name	si:ch211-11p18.6
General protein information	Preferred Names si:ch211-11p18.6 Names protein NLRC5 uncharacterized protein si:ch211-11p18.6 novel NACHT domain containing protein
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017353266.1	XP_017208755.1	uncharacterized protein si:ch211-11p18.6 isoform X7
XM_017353261.1	XP_017208750.1	uncharacterized protein si:ch211-11p18.6 isoform X2
XM_017353263.1	XP_017208752.1	protein NLRC5 isoform X4
XM_021469425.1	XP_021325100.1	uncharacterized protein si:ch211-11p18.6 isoform X5
XM_021469428.1	XP_021325103.1	uncharacterized protein si:ch211-11p18.6 isoform X8
XM_021469429.1	XP_021325104.1	uncharacterized protein si:ch211-11p18.6 isoform X9
XM_021469433.1	XP_021325108.1	protein NLRC5 isoform X14
XM_017353262.1	XP_017208751.1	uncharacterized protein si:ch211-11p18.6 isoform X3
XM_017353265.1	XP_017208754.1	uncharacterized protein si:ch211-11p18.6 isoform X6
XM_017353268.1	XP_017208757.1	protein NLRC5 isoform X9
XM_017353260.1	XP_017208749.1	uncharacterized protein si:ch211-11p18.6 isoform X1
XM_021469431.1	XP_021325106.1	uncharacterized protein si:ch211-11p18.6 isoform X12
XM_017353270.1	XP_017208759.1	protein NLRC5 isoform X11
XM_017353271.1	XP_017208760.1	protein NLRC5 isoform X12
XM_021469422.1	XP_021325097.1	uncharacterized protein si:ch211-11p18.6 isoform X2
XM_021469434.1	XP_021325109.1	protein NLRC5 isoform X15
XM_021469423.1	XP_021325098.1	uncharacterized protein si:ch211-11p18.6 isoform X3
XM_021469424.1	XP_021325099.1	uncharacterized protein si:ch211-11p18.6 isoform X4
XM_021469426.1	XP_021325101.1	uncharacterized protein si:ch211-11p18.6 isoform X6
XM_021469427.1	XP_021325102.1	uncharacterized protein si:ch211-11p18.6 isoform X7
XM_021469430.1	XP_021325105.1	uncharacterized protein si:ch211-11p18.6 isoform X11
XM_021469432.1	XP_021325107.1	protein NLRC5 isoform X13
XM_017353266.2	XP_017208755.1	uncharacterized protein si:ch211-11p18.6 isoform X10
XM_021469421.1	XP_021325096.1	uncharacterized protein si:ch211-11p18.6 isoform X1
XM_021469435.1	XP_021325110.1	protein NLRC5 isoform X16
XM_017353264.1	XP_017208753.1	protein NLRC5 isoform X5
XM_017353267.1	XP_017208756.1	protein NLRC5 isoform X8
XM_017353269.1	XP_017208758.1	protein NLRC5 isoform X10
XM_017353272.1	XP_017208761.1	protein NLRC5 isoform X13

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC565414	XP_693785.6
Danio rerio (zebrafish)	LOC558375	XP_686669.1
Danio rerio (zebrafish)	ftr29	XP_003198034.1
Danio rerio (zebrafish)	ftr82	NP_001068571.1
Danio rerio (zebrafish)	LOC560422	XP_688915.1
Danio rerio (zebrafish)	ftr73	XP_005157866.1
Danio rerio (zebrafish)	ftr11	XP_003197971.1
Danio rerio (zebrafish)	ftr67	NP_001034808.2
Danio rerio (zebrafish)	ftr37	XP_696445.3
Danio rerio (zebrafish)	btr18	XP_005173787.1
Danio rerio (zebrafish)	btr32	XP_003201305.1
Danio rerio (zebrafish)	btr07	XP_001923237.4
Danio rerio (zebrafish)	ftr79	XP_697299.2
Danio rerio (zebrafish)	ftr83	XP_688583.3
Danio rerio (zebrafish)	si:dkeyp-123a12.2	XP_691204.4
Danio rerio (zebrafish)	si:ch211-250k18.8	XP_698776.5
Danio rerio (zebrafish)	LOC566957	XP_005174040.1
Danio rerio (zebrafish)	si:ch211-196h16.5	XP_690098.5
Danio rerio (zebrafish)	si:ch211-194e1.7	XP_005167387.1
Danio rerio (zebrafish)	LOC793291	XP_001331884.5
Danio rerio (zebrafish)	si:ch211-114l13.1	XP_002660672.3
Danio rerio (zebrafish)	LOC566508	XP_694871.6
Danio rerio (zebrafish)	ftr55	XP_003200609.2
Danio rerio (zebrafish)	btr04	XP_003198956.2
Danio rerio (zebrafish)	LOC101883084	XP_005174455.1
Danio rerio (zebrafish)	LOC100003765	XP_001343238.2
Danio rerio (zebrafish)	LOC101883156	XP_005160435.1
Danio rerio (zebrafish)	LOC101883187	XP_005174457.1
Danio rerio (zebrafish)	si:rp71-7l19.2	XP_003198343.2
Danio rerio (zebrafish)	LOC101882306	XP_005171748.1
Danio rerio (zebrafish)	LOC100000832	XP_001340954.2
Danio rerio (zebrafish)	LOC100005516	XP_005171758.1
Danio rerio (zebrafish)	LOC100005625	XP_001339269.4
Danio rerio (zebrafish)	LOC100004711	XP_001340175.3
Danio rerio (zebrafish)	LOC101883426	XP_005174069.1
Danio rerio (zebrafish)	LOC101882512	XP_005171750.1
Danio rerio (zebrafish)	ftr49	XP_003198077.1
Danio rerio (zebrafish)	LOC101882560	XP_005163612.1
Danio rerio (zebrafish)	LOC100001589	XP_001341559.3
Danio rerio (zebrafish)	LOC100006636	XP_005172385.1
Danio rerio (zebrafish)	LOC101885873	XP_005161560.1
Danio rerio (zebrafish)	si:ch211-237a4.2	XP_003198575.2
Danio rerio (zebrafish)	LOC100007791	XP_001346158.5
Danio rerio (zebrafish)	LOC101884187	XP_005157862.1
Danio rerio (zebrafish)	LOC101884637	XP_005168127.1
Danio rerio (zebrafish)	LOC101885187	XP_005165034.1
Danio rerio (zebrafish)	zgc:174944	NP_001108057.1
Danio rerio (zebrafish)	btr22	XP_005173784.1
Danio rerio (zebrafish)	si:ch211-15j1.3	XP_002660683.2
Danio rerio (zebrafish)	LOC100150225	XP_001921257.4
Danio rerio (zebrafish)	LOC100150280	XP_005174567.1
Danio rerio (zebrafish)	btr09	NP_001124100.1
Danio rerio (zebrafish)	zgc:194906	NP_001124145.1
Danio rerio (zebrafish)	ftr34	XP_005163669.1
Danio rerio (zebrafish)	LOC100329306	XP_005168148.1
Danio rerio (zebrafish)	LOC100329543	XP_005167374.1
Danio rerio (zebrafish)	LOC100333853	XP_002661845.3
Danio rerio (zebrafish)	LOC100334101	XP_003197833.2
Danio rerio (zebrafish)	si:ch211-233g6.6	XP_002660707.3
Danio rerio (zebrafish)	LOC100334843	XP_002665168.2
Danio rerio (zebrafish)	LOC100331680	XP_002667178.3
Danio rerio (zebrafish)	si:ch73-24k9.2	XP_002665507.3
Danio rerio (zebrafish)	LOC100330813	XP_002660428.2
Danio rerio (zebrafish)	LOC100331097	XP_002661460.3
Danio rerio (zebrafish)	LOC100536980	XP_003201699.2
Danio rerio (zebrafish)	ftr30	XP_003198033.2
Danio rerio (zebrafish)	LOC100535633	XP_003198959.1
Danio rerio (zebrafish)	LOC100535915	XP_005161831.1
Danio rerio (zebrafish)	LOC100534746	XP_005161332.1
Danio rerio (zebrafish)	LOC558675	XP_687012.2
Danio rerio (zebrafish)	ftr16	XP_690796.5
Danio rerio (zebrafish)	LOC561829	XP_690320.2
Danio rerio (zebrafish)	ftr06	XP_001337856.1
Danio rerio (zebrafish)	LOC570185	XP_698724.1
Danio rerio (zebrafish)	LOC797531	XP_001338000.2
Danio rerio (zebrafish)	ftr02	XP_003197969.1
Danio rerio (zebrafish)	LOC571442	XP_700123.5
Danio rerio (zebrafish)	zgc:136302	XP_005170221.1
Danio rerio (zebrafish)	ftr58	XP_686163.5
Danio rerio (zebrafish)	ftr64	XP_002661885.1
Danio rerio (zebrafish)	btr31	XP_005170663.1
Danio rerio (zebrafish)	si:ch211-121a2.3	XP_698105.5
Danio rerio (zebrafish)	LOC799802	XP_001336792.2
Danio rerio (zebrafish)	LOC555438	XP_683030.6
Danio rerio (zebrafish)	LOC568716	XP_003201051.2
Danio rerio (zebrafish)	LOC568664	XP_003201050.2
Danio rerio (zebrafish)	wu:fb15h11	XP_697949.6
Danio rerio (zebrafish)	si:ch211-191a16.2	XP_001338550.5
Danio rerio (zebrafish)	LOC795182	XP_001332957.4
Danio rerio (zebrafish)	si:ch211-213a13.6	XP_697815.6
Danio rerio (zebrafish)	si:dkey-13n15.11	XP_005165261.1
Danio rerio (zebrafish)	zgc:136791	XP_005160238.1
Danio rerio (zebrafish)	LOC796214	XP_005161889.1
Danio rerio (zebrafish)	zgc:174178	NP_001107787.1
Danio rerio (zebrafish)	si:ch211-114l13.4	XP_002660681.1
Danio rerio (zebrafish)	si:dkey-222h21.8	XP_002660686.2
Danio rerio (zebrafish)	si:dkey-222h21.5	XP_002660689.1
Danio rerio (zebrafish)	si:ch211-255g12.8	XP_694303.5
Danio rerio (zebrafish)	LOC101882488	XP_005163611.1
Danio rerio (zebrafish)	LOC101882503	XP_005168090.1
Danio rerio (zebrafish)	LOC101882744	XP_005168242.1
Danio rerio (zebrafish)	LOC100002266	XP_005158672.1
Danio rerio (zebrafish)	LOC101883760	XP_005164630.1
Danio rerio (zebrafish)	ftr39p	XP_002661035.2
Danio rerio (zebrafish)	LOC101886579	XP_005161565.1
Danio rerio (zebrafish)	si:ch73-286h23.3	XP_001346239.5
Danio rerio (zebrafish)	LOC101886147	XP_005167368.1
Danio rerio (zebrafish)	LOC100006645	XP_001337665.3
Danio rerio (zebrafish)	LOC100006950	XP_001345543.5
Danio rerio (zebrafish)	LOC101884123	XP_005171093.1
Danio rerio (zebrafish)	ftr28l	XP_005163705.1
Danio rerio (zebrafish)	LOC101884874	XP_005171101.1
Danio rerio (zebrafish)	ftr50	XP_005164403.1
Danio rerio (zebrafish)	si:ch211-15j1.5	XP_002660682.2
Danio rerio (zebrafish)	si:ch211-15j1.1	XP_001920778.3
Danio rerio (zebrafish)	si:ch211-181d7.1	XP_001919795.3
Danio rerio (zebrafish)	LOC100334547	XP_002664742.2
Danio rerio (zebrafish)	LOC100334715	XP_005171097.1
Danio rerio (zebrafish)	si:ch211-278p9.3	XP_002665167.3
Danio rerio (zebrafish)	LOC100329650	XP_002661801.3
Danio rerio (zebrafish)	LOC100329836	XP_002664746.3
Danio rerio (zebrafish)	LOC100330744	XP_002660427.3
Danio rerio (zebrafish)	ftr76	XP_005157864.1
Danio rerio (zebrafish)	ftr19	XP_002660960.3
Danio rerio (zebrafish)	ftr03	XP_002660930.2
Danio rerio (zebrafish)	LOC100333264	XP_002660697.3
Danio rerio (zebrafish)	LOC100333325	XP_002667878.3
Danio rerio (zebrafish)	si:zfos-364h11.1	XP_002665163.2
Danio rerio (zebrafish)	LOC100333957	XP_005171081.1
Danio rerio (zebrafish)	si:dkey-156m2.3	XP_002664795.3
Danio rerio (zebrafish)	LOC100331576	XP_002661628.3
Danio rerio (zebrafish)	LOC100536939	XP_003200134.2
Danio rerio (zebrafish)	LOC100537283	XP_003198007.2
Danio rerio (zebrafish)	LOC793898	XP_001920428.2
Danio rerio (zebrafish)	LOC791496	XP_003198035.2
Danio rerio (zebrafish)	LOC565787	XP_694149.5
Danio rerio (zebrafish)	LOC570079	XP_698604.5
Danio rerio (zebrafish)	LOC570163	XP_003197979.1
Danio rerio (zebrafish)	btr16	XP_005157512.1
Danio rerio (zebrafish)	LOC570251	XP_698802.4
Danio rerio (zebrafish)	ftr15	XP_700104.3
Danio rerio (zebrafish)	ftr26	XP_005163617.1
Danio rerio (zebrafish)	ftr65	XP_003198031.2
Danio rerio (zebrafish)	ftr61	XP_003201315.1
Danio rerio (zebrafish)	btr20	XP_003200561.1
Danio rerio (zebrafish)	si:dkey-151g22.1	NP_001137518.1
Danio rerio (zebrafish)	si:dkey-121n8.4	XP_001335920.4
Danio rerio (zebrafish)	si:dkey-7i4.1	XP_005169721.1
Danio rerio (zebrafish)	LOC562679	XP_691146.5
Danio rerio (zebrafish)	LOC559196	XP_687605.5
Danio rerio (zebrafish)	LOC795109	XP_001335224.5
Danio rerio (zebrafish)	si:ch211-187g4.1	XP_698878.6
Danio rerio (zebrafish)	LOC571651	XP_700351.5
Danio rerio (zebrafish)	LOC565381	XP_693750.6
Danio rerio (zebrafish)	si:ch211-238e22.7	XP_005168160.1
Danio rerio (zebrafish)	trpm2	XP_003198602.2
Danio rerio (zebrafish)	btr12	NP_001070087.1
Danio rerio (zebrafish)	btr02	NP_001070626.2
Danio rerio (zebrafish)	LOC101883234	XP_005160282.1
Danio rerio (zebrafish)	LOC100002745	XP_005164415.1
Danio rerio (zebrafish)	LOC100001465	XP_005166707.1
Danio rerio (zebrafish)	LOC101883625	XP_005170693.1
Danio rerio (zebrafish)	LOC101883660	XP_005168155.1
Danio rerio (zebrafish)	LOC100005483	XP_001339130.3
Danio rerio (zebrafish)	LOC100006280	XP_001920132.4
Danio rerio (zebrafish)	LOC101884427	XP_005159614.1
Danio rerio (zebrafish)	LOC101886178	XP_005164994.1
Danio rerio (zebrafish)	LOC101886307	XP_005163673.1
Danio rerio (zebrafish)	LOC101885734	XP_005161559.1
Danio rerio (zebrafish)	LOC101885938	XP_005173831.1
Danio rerio (zebrafish)	LOC101885252	XP_005168541.1
Danio rerio (zebrafish)	zgc:173666	XP_003198037.1
Danio rerio (zebrafish)	zgc:173581	XP_005173811.1
Danio rerio (zebrafish)	LOC100329354	XP_002661501.3
Danio rerio (zebrafish)	LOC100150791	XP_005165031.1
Danio rerio (zebrafish)	btr06	NP_001120951.1
Danio rerio (zebrafish)	LOC100331072	XP_002661905.3
Danio rerio (zebrafish)	si:ch211-271g18.1	XP_002661463.3
Danio rerio (zebrafish)	si:dkey-222h21.6	XP_002660688.2
Danio rerio (zebrafish)	si:ch73-236c18.7	XP_002660699.3
Danio rerio (zebrafish)	LOC100333747	XP_002661530.3
Danio rerio (zebrafish)	LOC100333840	XP_005169858.1
Danio rerio (zebrafish)	si:ch211-233g6.3	XP_002660709.3
Danio rerio (zebrafish)	LOC100330073	XP_002661806.3
Danio rerio (zebrafish)	LOC100536924	XP_003200567.2
Danio rerio (zebrafish)	LOC100538217	XP_005169783.1
Danio rerio (zebrafish)	ftr72	NP_001007388.2
Danio rerio (zebrafish)	trim25l	XP_005172141.1
Danio rerio (zebrafish)	ftr56	XP_003200605.1
Danio rerio (zebrafish)	btr25	XP_005173808.1
Danio rerio (zebrafish)	ftr24	XP_003197988.1
Danio rerio (zebrafish)	ftr84	XP_005165427.1
Danio rerio (zebrafish)	ftr43	XP_005164401.1
Danio rerio (zebrafish)	LOC571488	XP_700178.4
Danio rerio (zebrafish)	ftr41	XP_695494.5
Danio rerio (zebrafish)	ftr59	XP_686796.2
Danio rerio (zebrafish)	ftr51	XP_691169.5
Danio rerio (zebrafish)	si:ch211-214b16.3	XP_005164324.1
Danio rerio (zebrafish)	si:dkey-222h21.2	XP_001334961.4
Danio rerio (zebrafish)	si:ch211-114l13.10	XP_696367.5
Danio rerio (zebrafish)	si:ch211-213a13.9	XP_003201049.2
Danio rerio (zebrafish)	si:dkey-253d23.10	XP_683789.6
Danio rerio (zebrafish)	si:ch211-278p9.4	XP_001336197.5
Danio rerio (zebrafish)	LOC566782	XP_695160.6
Danio rerio (zebrafish)	LOC558818	NP_001038341.1
Danio rerio (zebrafish)	si:ch211-149a19.3	XP_005170041.1
Danio rerio (zebrafish)	si:dkey-222h21.9	XP_002660685.1
Danio rerio (zebrafish)	LOC796962	XP_001923433.2
Danio rerio (zebrafish)	si:ch211-233m11.1	XP_687146.4
Danio rerio (zebrafish)	LOC794214	XP_001334125.1
Danio rerio (zebrafish)	si:dkey-126g1.7	XP_005162400.1
Danio rerio (zebrafish)	LOC565296	XP_003198495.2
Danio rerio (zebrafish)	si:dkeyp-4c4.1	NP_001094426.1
Danio rerio (zebrafish)	LOC560232	NP_001116726.1
Danio rerio (zebrafish)	si:ch211-114l13.13	XP_005160268.1
Danio rerio (zebrafish)	LOC558836	XP_003198237.2
Danio rerio (zebrafish)	LOC572555	XP_005164323.1
Danio rerio (zebrafish)	LOC799840	XP_001340155.1
Danio rerio (zebrafish)	LOC100001765	XP_001341704.4
Danio rerio (zebrafish)	LOC101882643	XP_005171752.1
Danio rerio (zebrafish)	LOC101883221	XP_005171452.1
Danio rerio (zebrafish)	LOC101886570	XP_005174223.1
Danio rerio (zebrafish)	LOC101884884	XP_005157350.1
Danio rerio (zebrafish)	LOC101884992	XP_005174036.1
Danio rerio (zebrafish)	LOC101883527	XP_005170222.1
Danio rerio (zebrafish)	LOC101883611	XP_005174070.1
Danio rerio (zebrafish)	LOC101886001	XP_005157342.1
Danio rerio (zebrafish)	LOC100007745	XP_001346125.5
Danio rerio (zebrafish)	si:ch211-150h8.3	XP_003198032.1
Danio rerio (zebrafish)	LOC101885169	XP_005174039.1
Danio rerio (zebrafish)	LOC101885212	XP_005158663.1
Danio rerio (zebrafish)	LOC101885461	XP_005168195.1
Danio rerio (zebrafish)	LOC100006564	NP_001119957.1
Danio rerio (zebrafish)	LOC101885760	XP_005164976.1
Danio rerio (zebrafish)	LOC100149991	XP_003200554.1
Danio rerio (zebrafish)	LOC100333746	XP_002661485.3
Danio rerio (zebrafish)	LOC100333748	XP_002661966.3
Danio rerio (zebrafish)	LOC100334029	XP_002660704.3
Danio rerio (zebrafish)	LOC100329448	XP_002667651.3
Danio rerio (zebrafish)	LOC100329480	XP_002663890.3
Danio rerio (zebrafish)	LOC100329558	XP_002661549.3
Danio rerio (zebrafish)	LOC100329927	XP_003200136.2
Danio rerio (zebrafish)	si:dkey-82i20.1	XP_002666374.3
Danio rerio (zebrafish)	LOC100334677	XP_002661542.3
Danio rerio (zebrafish)	LOC100334710	XP_002665516.2
Danio rerio (zebrafish)	btr21	XP_003200564.2
Danio rerio (zebrafish)	LOC100331159	XP_005169797.1
Danio rerio (zebrafish)	LOC100331532	XP_003201602.2
Danio rerio (zebrafish)	LOC100536895	XP_003200133.2
Danio rerio (zebrafish)	LOC100537076	XP_003200135.2

Gene Ontology

The following SI:CH211-11P18.6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI:CH211-11P18.6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa151255	XM_021469432.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa151256	XM_021469433.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa151257	XM_021469434.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa151258	XM_021469435.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa151251	XM_021469428.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa151252	XM_021469429.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa151253	XM_021469430.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa151254	XM_021469431.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa57137	XM_017353266.2 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa57138	XM_017353267.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa57139	XM_017353268.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa57140	XM_017353269.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa57141	XM_017353270.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa57142	XM_017353271.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa57143	XM_017353272.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa151244	XM_021469421.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa151245	XM_021469422.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa151246	XM_021469423.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa151247	XM_021469424.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa151248	XM_021469425.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa151249	XM_021469426.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa151250	XM_021469427.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa57131	XM_017353260.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa57132	XM_017353261.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa57133	XM_017353262.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa57134	XM_017353263.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa57135	XM_017353264.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa57136	XM_017353265.1 Latest version!	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
ODa57137	XM_017353266.1	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI:CH211-11P18.6 ( XM_021469432.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325107.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X13, mRNA.

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CloneID	ODa151255
Clone ID Related Accession (Same CDS sequence)	XM_021469432.1
Accession Version	XM_021469432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	protein NLRC5 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACAACGGGGT GATTGCTGTA AATTAAAAAC CGTCAGGTTT 
TTAAAGAGTT CTGCTGCACA GGAAGCGTGT GACTATCTTA CTAAAGCTGT GGGTAAAAGT 
CCATTATTAC TGACAAAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAA GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTGAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAGATCTGT 
GAGGGGCTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ACCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACTTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGAAGCT CACTGACTTA 
CTGCAGGATC CACACTGTAC CCTGAATACA CTGAGGTTTT TGAGTCCTGC CGCAGAAGAA 
GCCTGTCAGT TTGTGACTGG AATTGTGGGT AAAAACCCGA TACTTCTGAA AGAACTGAAT 
CTGAGTGATC ATGAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AAAAACAGCT TTATCTCAGA GGAAGATTGT 
CGTGTTCTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGAGAGAGCT GAATTTGAAT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCAACA CTCTGTTTCA AAACCAACAG 
TGCAGACTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT 
TTGACTTCAG CATTTAACTC AAACCCTTCA CACCTGAAAG AGCTCAATCT GAGTGGGAAT 
AAACTAGGAG ACTCAGGAGT AAGAAAAATC TCCAGTCTAC TGAGAAACTC GCAGTGCATG 
CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTTACATCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACCATCAG GAGTGAAGGA GCTCACTGGC TTACTACTCG ATCCACACTG TACACTGACG 
ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GAATTCTGTC AGTTTGTGTC TGGAATTGTG 
GGTAAATACC CATTACTCCT GAAAGAACTG AATCTGAGTG ATCGTGGACT AGGGGACACA 
CGAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT TTAGACTCAA CACACTCATG 
CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TAACTTCAGC ATTTAATTTA 
AATCCTTCAC ACCTGATAGA GCTCAATCTG AGTGGGAATA AACTAGAAGA CTCAGGAGTA 
GAGAAGATCT GTCCTCTGCT GGAAAATATA CAATGCCGAT TAGAGAAACT GAGTCTTTCA 
GACTGCAGTA TCAGGGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGATT TACTGCAGAG TAGAAGCTGT AAATTCAGGT TTCTGAAGAG TTCTGCTGCA 
CAGGAAGCAT GCGAGTATCT TACTAATGTT CTGGGTATAA GTCCATTATT ACTGACAGAA 
CTGGATCTGA GTGAAGTTAA ATTAGGAGAT CTGGACTGGG AGAAACTCTC TGCTCTTCTG 
ATGGACTCTC ATAGCAAAGT GGAGAAAATC AAGCTAAATA ATTGTGAGCT GACAGAGAAA 
AGCTGTTCAG TTCTAGCTGC TGTTCTCTCC TCCAAAACCA TCCTGAAAGA GCTGAACCTG 
AACAACAGTC GTCTGCTGGA CTCAGGAGTC AGAAAGATCT GTGAGGGACT GAAGAATCCT 
GTGTGTGAGC TGAAGATACT CAATCTTTCA GACTGCAGTA TTACAGAAGA AGGTTATGAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGGAG CTCACTGAGT TACTGCAGGA TCCACACTGT 
GCACTGAAGA CACTGAGATT TTTAAGAAGT CCTGCTGCTG ATGAAGGCTG TCAGTTAGTA 
ACTGGAGTTG TGGGTAAAAA CCCGTTACTC CTGAAGGAAC TGAATCTGAG TGATCATGAG 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGCCAGATC 
AACACACTCA ATCTGAGTTA TTGCAGTATT ACAGAGAAAC AGTGTGAGAT CCTGACTTCA 
GCTCTGAAAT CAAACCCATC ACACCTGAGA GAACTGGACC TCAGTGGGAA TGAACTAGGA 
GACTTTCAAA GCCTTGGCTC TCTACTGAAG AACCAACAAT TCAAGCTGGA GAAACTAATT 
CTGTGTGTTT GCAGTATTAA AGAGAAGCAG TGTGAGATCC TGACTTCAGC TCTGAAATCA 
AACCCATCAC ACCTGAGAGA ACTGAACCTC AGTGGGAATG AACTCAAGCA CAGAAGAGTG 
AAGCAATTTT TTGATGTGCT GAAAAAATCA ACCTGTAAAC TGGAGCGACT GAGGTTAAGA 
TACTGTAGTA TACAAGCCGA TGATTGTACT GATCTGGCTT CAGCTCTGAA ATCAAACCCA 
TCCCACCTGA GAGAACTGGA CCTCAGTGGA AACAATCTAG GAGACTCTGG AGTGAAAAAA 
CTCAGTGATT TACTGATAAA CCAACAATTC AAGCTGGAGA AACTACGTCT GAGTTATTGC 
AGTATTACAG AGAAACAGTG TGTGATCCTG ACTTCAGCTC TGAAATCAAA CCCATCACAC 
CTGAGAGAAC TGAACCTCAA TGGGAATAGA CTAGGAGACT CTGGAGCTGA TGGCCTTAAC 
CATCTGCTGA GCAGTGTGGA TTGCAAGCTA AATAAACTAC ATCTGTGTGA TTGCAGTATT 
ACAGAGAAAC AGTTGCTCAT CCCAATCACA GCTACGTGTA TGGGCCTATT ACACCTGAGA 
GAACTGGACC TCAGTAAGAA CCAAATAAAA AACCAAGGAG TGTACATGCT GTGTGATGTG 
CTGAAAAACC CACGCTGTAG ACTCAAGAGG TTAAGACTAA ATGACTGTGG CATTACAGAT 
GTTACACCCT TAACTCAGTC TTTGACCAAG GACAAAGCAT TGAAGTTCTT AAAAGAGCTT 
GAACTGAGTA AAAATCAAAT GGGATTCTTC GAAAAGCAAA AACTCAGTGA CCTGCTGCGA 
GACTCAAACT GTAAACTGAG GCTTGAGGAA GAAGAAGGTT ATTTTAGAGC TGGTGTAAAA 
CGGGTTACTA CATTTTTGAT GCCTCCATGG TCAAAAGTTC AAAAACCTGT GGATCTCACA 
GGAGAGGAAA GTTCATCATC AGATGAGGAC GCCTCTGAAG ATGAGACTGA TCAGGCAGAG 
GAGGCTGATA AGCTCTCAAA TGAGGCGGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325107.1
CDS	65..5677
Translation	MSNNNLQDSGVKLLCTALKNVKCELEVLRISYCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGILGKNPLLLKELNLSDCELGDTGVNQLSALLQDKHCKPKTLMLRGCGLKEETCSALATVLGSDTSLKELDMSNNNLQDLGVKKLQSGLENTNCTLEKLSLSDCNISEEGYKALASALRSNPSHLIELNLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAAEEFCQFVTGIVGKNPLLLKELYLSDHELGDTRVNQLSALLQDKHCTVSTLILHNCGLTKESCSALASALSSDTSLKELDLCNNNLQDSGVKKLQSELQNTNWRLKKLRLSKCDLTEESCSALASVLTSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSKLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELIELLQDPLCTLKTLRFLSPAADEACQFVTGIVGKNLLLLKELNLSDRQLGDTRVNQLSALLQDKHCTLNTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_021469432.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469432.1

ORF Insert Sequence:

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACAACGGGGT GATTGCTGTA AATTAAAAAC CGTCAGGTTT 
TTAAAGAGTT CTGCTGCACA GGAAGCGTGT GACTATCTTA CTAAAGCTGT GGGTAAAAGT 
CCATTATTAC TGACAAAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAA GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTGAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAGATCTGT 
GAGGGGCTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ACCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACTTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGAAGCT CACTGACTTA 
CTGCAGGATC CACACTGTAC CCTGAATACA CTGAGGTTTT TGAGTCCTGC CGCAGAAGAA 
GCCTGTCAGT TTGTGACTGG AATTGTGGGT AAAAACCCGA TACTTCTGAA AGAACTGAAT 
CTGAGTGATC ATGAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AAAAACAGCT TTATCTCAGA GGAAGATTGT 
CGTGTTCTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGAGAGAGCT GAATTTGAAT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCAACA CTCTGTTTCA AAACCAACAG 
TGCAGACTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT 
TTGACTTCAG CATTTAACTC AAACCCTTCA CACCTGAAAG AGCTCAATCT GAGTGGGAAT 
AAACTAGGAG ACTCAGGAGT AAGAAAAATC TCCAGTCTAC TGAGAAACTC GCAGTGCATG 
CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTTACATCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACCATCAG GAGTGAAGGA GCTCACTGGC TTACTACTCG ATCCACACTG TACACTGACG 
ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GAATTCTGTC AGTTTGTGTC TGGAATTGTG 
GGTAAATACC CATTACTCCT GAAAGAACTG AATCTGAGTG ATCGTGGACT AGGGGACACA 
CGAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT TTAGACTCAA CACACTCATG 
CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TAACTTCAGC ATTTAATTTA 
AATCCTTCAC ACCTGATAGA GCTCAATCTG AGTGGGAATA AACTAGAAGA CTCAGGAGTA 
GAGAAGATCT GTCCTCTGCT GGAAAATATA CAATGCCGAT TAGAGAAACT GAGTCTTTCA 
GACTGCAGTA TCAGGGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGATT TACTGCAGAG TAGAAGCTGT AAATTCAGGT TTCTGAAGAG TTCTGCTGCA 
CAGGAAGCAT GCGAGTATCT TACTAATGTT CTGGGTATAA GTCCATTATT ACTGACAGAA 
CTGGATCTGA GTGAAGTTAA ATTAGGAGAT CTGGACTGGG AGAAACTCTC TGCTCTTCTG 
ATGGACTCTC ATAGCAAAGT GGAGAAAATC AAGCTAAATA ATTGTGAGCT GACAGAGAAA 
AGCTGTTCAG TTCTAGCTGC TGTTCTCTCC TCCAAAACCA TCCTGAAAGA GCTGAACCTG 
AACAACAGTC GTCTGCTGGA CTCAGGAGTC AGAAAGATCT GTGAGGGACT GAAGAATCCT 
GTGTGTGAGC TGAAGATACT CAATCTTTCA GACTGCAGTA TTACAGAAGA AGGTTATGAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGGAG CTCACTGAGT TACTGCAGGA TCCACACTGT 
GCACTGAAGA CACTGAGATT TTTAAGAAGT CCTGCTGCTG ATGAAGGCTG TCAGTTAGTA 
ACTGGAGTTG TGGGTAAAAA CCCGTTACTC CTGAAGGAAC TGAATCTGAG TGATCATGAG 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGCCAGATC 
AACACACTCA ATCTGAGTTA TTGCAGTATT ACAGAGAAAC AGTGTGAGAT CCTGACTTCA 
GCTCTGAAAT CAAACCCATC ACACCTGAGA GAACTGGACC TCAGTGGGAA TGAACTAGGA 
GACTTTCAAA GCCTTGGCTC TCTACTGAAG AACCAACAAT TCAAGCTGGA GAAACTAATT 
CTGTGTGTTT GCAGTATTAA AGAGAAGCAG TGTGAGATCC TGACTTCAGC TCTGAAATCA 
AACCCATCAC ACCTGAGAGA ACTGAACCTC AGTGGGAATG AACTCAAGCA CAGAAGAGTG 
AAGCAATTTT TTGATGTGCT GAAAAAATCA ACCTGTAAAC TGGAGCGACT GAGGTTAAGA 
TACTGTAGTA TACAAGCCGA TGATTGTACT GATCTGGCTT CAGCTCTGAA ATCAAACCCA 
TCCCACCTGA GAGAACTGGA CCTCAGTGGA AACAATCTAG GAGACTCTGG AGTGAAAAAA 
CTCAGTGATT TACTGATAAA CCAACAATTC AAGCTGGAGA AACTACGTCT GAGTTATTGC 
AGTATTACAG AGAAACAGTG TGTGATCCTG ACTTCAGCTC TGAAATCAAA CCCATCACAC 
CTGAGAGAAC TGAACCTCAA TGGGAATAGA CTAGGAGACT CTGGAGCTGA TGGCCTTAAC 
CATCTGCTGA GCAGTGTGGA TTGCAAGCTA AATAAACTAC ATCTGTGTGA TTGCAGTATT 
ACAGAGAAAC AGTTGCTCAT CCCAATCACA GCTACGTGTA TGGGCCTATT ACACCTGAGA 
GAACTGGACC TCAGTAAGAA CCAAATAAAA AACCAAGGAG TGTACATGCT GTGTGATGTG 
CTGAAAAACC CACGCTGTAG ACTCAAGAGG TTAAGACTAA ATGACTGTGG CATTACAGAT 
GTTACACCCT TAACTCAGTC TTTGACCAAG GACAAAGCAT TGAAGTTCTT AAAAGAGCTT 
GAACTGAGTA AAAATCAAAT GGGATTCTTC GAAAAGCAAA AACTCAGTGA CCTGCTGCGA 
GACTCAAACT GTAAACTGAG GCTTGAGGAA GAAGAAGGTT ATTTTAGAGC TGGTGTAAAA 
CGGGTTACTA CATTTTTGAT GCCTCCATGG TCAAAAGTTC AAAAACCTGT GGATCTCACA 
GGAGAGGAAA GTTCATCATC AGATGAGGAC GCCTCTGAAG ATGAGACTGA TCAGGCAGAG 
GAGGCTGATA AGCTCTCAAA TGAGGCGGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_021469433.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325108.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X14, mRNA.

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CloneID	ODa151256
Clone ID Related Accession (Same CDS sequence)	XM_021469433.1
Accession Version	XM_021469433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	protein NLRC5 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACAACGGGGT GATTGCTGTA AATTAAAAAC CGTCAGGTTT 
TTAAAGAGTT CTGCTGCACA GGAAGCGTGT GACTATCTTA CTAAAGCTGT GGGTAAAAGT 
CCATTATTAC TGACAAAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAA GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTGAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAGATCTGT 
GAGGGGCTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ACCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACTTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGAAGCT CACTGACTTA 
CTGCAGGATC CACACTGTAC CCTGAATACA CTGAGGTTTT TGAGTCCTGC CGCAGAAGAA 
GCCTGTCAGT TTGTGACTGG AATTGTGGGT AAAAACCCGA TACTTCTGAA AGAACTGAAT 
CTGAGTGATC ATGAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AAAAACAGCT TTATCTCAGA GGAAGATTGT 
CGTGTTCTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGAGAGAGCT GAATTTGAAT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCAACA CTCTGTTTCA AAACCAACAG 
TGCAGACTGG AAAAGCTGAA GTTTAGCGGC ATCAGTATCA CAGCAGAAGG TTGTGCTGCT 
TTGACTTCAG CATTTAGCTC AAACCCGTCA CACCTGATAG AGCTAAATCT GAGTGGGAAT 
ACACTAGAAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC ACAGTGCATG 
CTGAAGATAC TCAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGCT TTGATTCCAG AGTGTGCTCT GAAAACATTG 
AGGTTTTTGG GTCCTGCAGC AGAAGAAGCC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCGTTAC TCCTGAAAGA ACTGAATCTG AGTGATCGTA AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC CCAACACACT TCAGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAA TTTAAATCCT 
TCACACCTGA TAGAGCTCAA TCTGAGTGGG AATAAACTAG AAGACTCAGG AGTAGAGAAG 
ATCTGTCCTC TGCTGGAAAA TATACAATGC CGATTAGAGA AACTGAGTCT TTCAGACTGC 
AGTATCAGGG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GATTTACTGC AGAGTAGAAG CTGTAAATTC AGGTTTCTGA AGAGTTCTGC TGCACAGGAA 
GCATGCGAGT ATCTTACTAA TGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT 
CTGAGTGAAG TTAAATTAGG AGATCTGGAC TGGGAGAAAC TCTCTGCTCT TCTGATGGAC 
TCTCATAGCA AAGTGGAGAA AATCAAGCTA AATAATTGTG AGCTGACAGA GAAAAGCTGT 
TCAGTTCTAG CTGCTGTTCT CTCCTCCAAA ACCATCCTGA AAGAGCTGAA CCTGAACAAC 
AGTCGTCTGC TGGACTCAGG AGTCAGAAAG ATCTGTGAGG GACTGAAGAA TCCTGTGTGT 
GAGCTGAAGA TACTCAATCT TTCAGACTGC AGTATTACAG AAGAAGGTTA TGAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GAGTTACTGC AGGATCCACA CTGTGCACTG 
AAGACACTGA GATTTTTAAG AAGTCCTGCT GCTGATGAAG GCTGTCAGTT AGTAACTGGA 
GTTGTGGGTA AAAACCCGTT ACTCCTGAAG GAACTGAATC TGAGTGATCA TGAGCTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGCCA GATCAACACA 
CTCAATCTGA GTTATTGCAG TATTACAGAG AAACAGTGTG AGATCCTGAC TTCAGCTCTG 
AAATCAAACC CATCACACCT GAGAGAACTG GACCTCAGTG GGAATGAACT AGGAGACTTT 
CAAAGCCTTG GCTCTCTACT GAAGAACCAA CAATTCAAGC TGGAGAAACT AATTCTGTGT 
GTTTGCAGTA TTAAAGAGAA GCAGTGTGAG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGAA CCTCAGTGGG AATGAACTCA AGCACAGAAG AGTGAAGCAA 
TTTTTTGATG TGCTGAAAAA ATCAACCTGT AAACTGGAGC GACTGAGGTT AAGATACTGT 
AGTATACAAG CCGATGATTG TACTGATCTG GCTTCAGCTC TGAAATCAAA CCCATCCCAC 
CTGAGAGAAC TGGACCTCAG TGGAAACAAT CTAGGAGACT CTGGAGTGAA AAAACTCAGT 
GATTTACTGA TAAACCAACA ATTCAAGCTG GAGAAACTAC GTCTGAGTTA TTGCAGTATT 
ACAGAGAAAC AGTGTGTGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA 
GAACTGAACC TCAATGGGAA TAGACTAGGA GACTCTGGAG CTGATGGCCT TAACCATCTG 
CTGAGCAGTG TGGATTGCAA GCTAAATAAA CTACATCTGT GTGATTGCAG TATTACAGAG 
AAACAGTTGC TCATCCCAAT CACAGCTACG TGTATGGGCC TATTACACCT GAGAGAACTG 
GACCTCAGTA AGAACCAAAT AAAAAACCAA GGAGTGTACA TGCTGTGTGA TGTGCTGAAA 
AACCCACGCT GTAGACTCAA GAGGTTAAGA CTAAATGACT GTGGCATTAC AGATGTTACA 
CCCTTAACTC AGTCTTTGAC CAAGGACAAA GCATTGAAGT TCTTAAAAGA GCTTGAACTG 
AGTAAAAATC AAATGGGATT CTTCGAAAAG CAAAAACTCA GTGACCTGCT GCGAGACTCA 
AACTGTAAAC TGAGGCTTGA GGAAGAAGAA GGTTATTTTA GAGCTGGTGT AAAACGGGTT 
ACTACATTTT TGATGCCTCC ATGGTCAAAA GTTCAAAAAC CTGTGGATCT CACAGGAGAG 
GAAAGTTCAT CATCAGATGA GGACGCCTCT GAAGATGAGA CTGATCAGGC AGAGGAGGCT 
GATAAGCTCT CAAATGAGGC GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325108.1
CDS	64..5670
Translation	MSNNNLQDSGVKLLCTALKNVKCELEVLRISYCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGILGKNPLLLKELNLSDCELGDTGVNQLSALLQDKHCKPKTLMLRGCGLKEETCSALATVLGSDTSLKELDMSNNNLQDLGVKKLQSGLENTNCTLEKLSLSDCNISEEGYKALASALRSNPSHLIELNLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAAEEFCQFVTGIVGKNPLLLKELYLSDHELGDTRVNQLSALLQDKHCTVSTLILHNCGLTKESCSALASALSSDTSLKELDLCNNNLQDSGVKKLQSELQNTNWRLKKLRLSKCDLTEESCSALASVLTSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSKLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELIELLQDPLCTLKTLRFLSPAADEACQFVTGIVGKNLLLLKELNLSDRQLGDTRVNQLSALLQDKHCTLNTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLKFSGISITAEGCAALTSAFSSNPSHLIELNLSGNTLEDSGVTEISSLLANSQCMLKILSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_021469433.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469433.1

ORF Insert Sequence:

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACAACGGGGT GATTGCTGTA AATTAAAAAC CGTCAGGTTT 
TTAAAGAGTT CTGCTGCACA GGAAGCGTGT GACTATCTTA CTAAAGCTGT GGGTAAAAGT 
CCATTATTAC TGACAAAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAA GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTGAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAGATCTGT 
GAGGGGCTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ACCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACTTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGAAGCT CACTGACTTA 
CTGCAGGATC CACACTGTAC CCTGAATACA CTGAGGTTTT TGAGTCCTGC CGCAGAAGAA 
GCCTGTCAGT TTGTGACTGG AATTGTGGGT AAAAACCCGA TACTTCTGAA AGAACTGAAT 
CTGAGTGATC ATGAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AAAAACAGCT TTATCTCAGA GGAAGATTGT 
CGTGTTCTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGAGAGAGCT GAATTTGAAT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCAACA CTCTGTTTCA AAACCAACAG 
TGCAGACTGG AAAAGCTGAA GTTTAGCGGC ATCAGTATCA CAGCAGAAGG TTGTGCTGCT 
TTGACTTCAG CATTTAGCTC AAACCCGTCA CACCTGATAG AGCTAAATCT GAGTGGGAAT 
ACACTAGAAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC ACAGTGCATG 
CTGAAGATAC TCAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGCT TTGATTCCAG AGTGTGCTCT GAAAACATTG 
AGGTTTTTGG GTCCTGCAGC AGAAGAAGCC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCGTTAC TCCTGAAAGA ACTGAATCTG AGTGATCGTA AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC CCAACACACT TCAGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAA TTTAAATCCT 
TCACACCTGA TAGAGCTCAA TCTGAGTGGG AATAAACTAG AAGACTCAGG AGTAGAGAAG 
ATCTGTCCTC TGCTGGAAAA TATACAATGC CGATTAGAGA AACTGAGTCT TTCAGACTGC 
AGTATCAGGG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GATTTACTGC AGAGTAGAAG CTGTAAATTC AGGTTTCTGA AGAGTTCTGC TGCACAGGAA 
GCATGCGAGT ATCTTACTAA TGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT 
CTGAGTGAAG TTAAATTAGG AGATCTGGAC TGGGAGAAAC TCTCTGCTCT TCTGATGGAC 
TCTCATAGCA AAGTGGAGAA AATCAAGCTA AATAATTGTG AGCTGACAGA GAAAAGCTGT 
TCAGTTCTAG CTGCTGTTCT CTCCTCCAAA ACCATCCTGA AAGAGCTGAA CCTGAACAAC 
AGTCGTCTGC TGGACTCAGG AGTCAGAAAG ATCTGTGAGG GACTGAAGAA TCCTGTGTGT 
GAGCTGAAGA TACTCAATCT TTCAGACTGC AGTATTACAG AAGAAGGTTA TGAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GAGTTACTGC AGGATCCACA CTGTGCACTG 
AAGACACTGA GATTTTTAAG AAGTCCTGCT GCTGATGAAG GCTGTCAGTT AGTAACTGGA 
GTTGTGGGTA AAAACCCGTT ACTCCTGAAG GAACTGAATC TGAGTGATCA TGAGCTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGCCA GATCAACACA 
CTCAATCTGA GTTATTGCAG TATTACAGAG AAACAGTGTG AGATCCTGAC TTCAGCTCTG 
AAATCAAACC CATCACACCT GAGAGAACTG GACCTCAGTG GGAATGAACT AGGAGACTTT 
CAAAGCCTTG GCTCTCTACT GAAGAACCAA CAATTCAAGC TGGAGAAACT AATTCTGTGT 
GTTTGCAGTA TTAAAGAGAA GCAGTGTGAG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGAA CCTCAGTGGG AATGAACTCA AGCACAGAAG AGTGAAGCAA 
TTTTTTGATG TGCTGAAAAA ATCAACCTGT AAACTGGAGC GACTGAGGTT AAGATACTGT 
AGTATACAAG CCGATGATTG TACTGATCTG GCTTCAGCTC TGAAATCAAA CCCATCCCAC 
CTGAGAGAAC TGGACCTCAG TGGAAACAAT CTAGGAGACT CTGGAGTGAA AAAACTCAGT 
GATTTACTGA TAAACCAACA ATTCAAGCTG GAGAAACTAC GTCTGAGTTA TTGCAGTATT 
ACAGAGAAAC AGTGTGTGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA 
GAACTGAACC TCAATGGGAA TAGACTAGGA GACTCTGGAG CTGATGGCCT TAACCATCTG 
CTGAGCAGTG TGGATTGCAA GCTAAATAAA CTACATCTGT GTGATTGCAG TATTACAGAG 
AAACAGTTGC TCATCCCAAT CACAGCTACG TGTATGGGCC TATTACACCT GAGAGAACTG 
GACCTCAGTA AGAACCAAAT AAAAAACCAA GGAGTGTACA TGCTGTGTGA TGTGCTGAAA 
AACCCACGCT GTAGACTCAA GAGGTTAAGA CTAAATGACT GTGGCATTAC AGATGTTACA 
CCCTTAACTC AGTCTTTGAC CAAGGACAAA GCATTGAAGT TCTTAAAAGA GCTTGAACTG 
AGTAAAAATC AAATGGGATT CTTCGAAAAG CAAAAACTCA GTGACCTGCT GCGAGACTCA 
AACTGTAAAC TGAGGCTTGA GGAAGAAGAA GGTTATTTTA GAGCTGGTGT AAAACGGGTT 
ACTACATTTT TGATGCCTCC ATGGTCAAAA GTTCAAAAAC CTGTGGATCT CACAGGAGAG 
GAAAGTTCAT CATCAGATGA GGACGCCTCT GAAGATGAGA CTGATCAGGC AGAGGAGGCT 
GATAAGCTCT CAAATGAGGC GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_021469434.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325109.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X15, mRNA.

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CloneID	ODa151257
Clone ID Related Accession (Same CDS sequence)	XM_021469434.1
Accession Version	XM_021469434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	protein NLRC5 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACAACGGGGT GATTGCTGTA AATTAAAAAC CGTCAGGTTT 
TTAAAGAGTT CTGCTGCACA GGAAGCGTGT GACTATCTTA CTAAAGCTGT GGGTAAAAGT 
CCATTATTAC TGACAAAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAA GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTGAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAGATCTGT 
GAGGGGCTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ACCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACTTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGAAGCT CACTGACTTA 
CTGCAGGATC CACACTGTAC CCTGAATACA CTGAGGTTTT TGAGTCCTGC CGCAGAAGAA 
GCCTGTCAGT TTGTGACTGG AATTGTGGGT AAAAACCCGA TACTTCTGAA AGAACTGAAT 
CTGAGTGATC ATGAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AAAAACAGCT TTATCTCAGA GGAAGATTGT 
CGTGTTCTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGAGAGAGCT GAATTTGAAT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCAACA CTCTGTTTCA AAACCAACAG 
TGCAGACTGG AAAAGCTGAA CAGCATGTGT ATTACAGCAG AAGGTTGTGC TGCTTTGACT 
TCAGCATTTA ACTCAAACCC TTCACACCTG AAAGAGCTCA ATCTGAGTGG GAATAAACTA 
GGAGACTCAG GAGTAAGAAA AATCTCCAGT CTACTGAGAA ACTCGCAGTG CATGCTGAAG 
ATACTCGGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTTACA TCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGGCTTACTA CTCGATCCAC ACTGTACACT GACGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAATTC TGTCAGTTTG TGTCTGGAAT TGTGGGTAAA 
TACCCATTAC TCCTGAAAGA ACTGAATCTG AGTGATCGTG GACTAGGGGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTTTAGAC TCAACACACT CATGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAA TTTAAATCCT 
TCACACCTGA TAGAGCTCAA TCTGAGTGGG AATAAACTAG AAGACTCAGG AGTAGAGAAG 
ATCTGTCCTC TGCTGGAAAA TATACAATGC CGATTAGAGA AACTGAGTCT TTCAGACTGC 
AGTATCAGGG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GATTTACTGC AGAGTAGAAG CTGTAAATTC AGGTTTCTGA AGAGTTCTGC TGCACAGGAA 
GCATGCGAGT ATCTTACTAA TGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT 
CTGAGTGAAG TTAAATTAGG AGATCTGGAC TGGGAGAAAC TCTCTGCTCT TCTGATGGAC 
TCTCATAGCA AAGTGGAGAA AATCAAGCTA AATAATTGTG AGCTGACAGA GAAAAGCTGT 
TCAGTTCTAG CTGCTGTTCT CTCCTCCAAA ACCATCCTGA AAGAGCTGAA CCTGAACAAC 
AGTCGTCTGC TGGACTCAGG AGTCAGAAAG ATCTGTGAGG GACTGAAGAA TCCTGTGTGT 
GAGCTGAAGA TACTCAATCT TTCAGACTGC AGTATTACAG AAGAAGGTTA TGAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GAGTTACTGC AGGATCCACA CTGTGCACTG 
AAGACACTGA GATTTTTAAG AAGTCCTGCT GCTGATGAAG GCTGTCAGTT AGTAACTGGA 
GTTGTGGGTA AAAACCCGTT ACTCCTGAAG GAACTGAATC TGAGTGATCA TGAGCTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGCCA GATCAACACA 
CTCAATCTGA GTTATTGCAG TATTACAGAG AAACAGTGTG AGATCCTGAC TTCAGCTCTG 
AAATCAAACC CATCACACCT GAGAGAACTG GACCTCAGTG GGAATGAACT AGGAGACTTT 
CAAAGCCTTG GCTCTCTACT GAAGAACCAA CAATTCAAGC TGGAGAAACT AATTCTGTGT 
GTTTGCAGTA TTAAAGAGAA GCAGTGTGAG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGAA CCTCAGTGGG AATGAACTCA AGCACAGAAG AGTGAAGCAA 
TTTTTTGATG TGCTGAAAAA ATCAACCTGT AAACTGGAGC GACTGAGGTT AAGATACTGT 
AGTATACAAG CCGATGATTG TACTGATCTG GCTTCAGCTC TGAAATCAAA CCCATCCCAC 
CTGAGAGAAC TGGACCTCAG TGGAAACAAT CTAGGAGACT CTGGAGTGAA AAAACTCAGT 
GATTTACTGA TAAACCAACA ATTCAAGCTG GAGAAACTAC GTCTGAGTTA TTGCAGTATT 
ACAGAGAAAC AGTGTGTGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA 
GAACTGAACC TCAATGGGAA TAGACTAGGA GACTCTGGAG CTGATGGCCT TAACCATCTG 
CTGAGCAGTG TGGATTGCAA GCTAAATAAA CTACATCTGT GTGATTGCAG TATTACAGAG 
AAACAGTTGC TCATCCCAAT CACAGCTACG TGTATGGGCC TATTACACCT GAGAGAACTG 
GACCTCAGTA AGAACCAAAT AAAAAACCAA GGAGTGTACA TGCTGTGTGA TGTGCTGAAA 
AACCCACGCT GTAGACTCAA GAGGTTAAGA CTAAATGACT GTGGCATTAC AGATGTTACA 
CCCTTAACTC AGTCTTTGAC CAAGGACAAA GCATTGAAGT TCTTAAAAGA GCTTGAACTG 
AGTAAAAATC AAATGGGATT CTTCGAAAAG CAAAAACTCA GTGACCTGCT GCGAGACTCA 
AACTGTAAAC TGAGGCTTGA GGAAGAAGAA GGTTATTTTA GAGCTGGTGT AAAACGGGTT 
ACTACATTTT TGATGCCTCC ATGGTCAAAA GTTCAAAAAC CTGTGGATCT CACAGGAGAG 
GAAAGTTCAT CATCAGATGA GGACGCCTCT GAAGATGAGA CTGATCAGGC AGAGGAGGCT 
GATAAGCTCT CAAATGAGGC GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325109.1
CDS	65..5671
Translation	MSNNNLQDSGVKLLCTALKNVKCELEVLRISYCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGILGKNPLLLKELNLSDCELGDTGVNQLSALLQDKHCKPKTLMLRGCGLKEETCSALATVLGSDTSLKELDMSNNNLQDLGVKKLQSGLENTNCTLEKLSLSDCNISEEGYKALASALRSNPSHLIELNLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAAEEFCQFVTGIVGKNPLLLKELYLSDHELGDTRVNQLSALLQDKHCTVSTLILHNCGLTKESCSALASALSSDTSLKELDLCNNNLQDSGVKKLQSELQNTNWRLKKLRLSKCDLTEESCSALASVLTSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSKLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELIELLQDPLCTLKTLRFLSPAADEACQFVTGIVGKNLLLLKELNLSDRQLGDTRVNQLSALLQDKHCTLNTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLNSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_021469434.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469434.1

ORF Insert Sequence:

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACAACGGGGT GATTGCTGTA AATTAAAAAC CGTCAGGTTT 
TTAAAGAGTT CTGCTGCACA GGAAGCGTGT GACTATCTTA CTAAAGCTGT GGGTAAAAGT 
CCATTATTAC TGACAAAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAA GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTGAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAGATCTGT 
GAGGGGCTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ACCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACTTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGAAGCT CACTGACTTA 
CTGCAGGATC CACACTGTAC CCTGAATACA CTGAGGTTTT TGAGTCCTGC CGCAGAAGAA 
GCCTGTCAGT TTGTGACTGG AATTGTGGGT AAAAACCCGA TACTTCTGAA AGAACTGAAT 
CTGAGTGATC ATGAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AAAAACAGCT TTATCTCAGA GGAAGATTGT 
CGTGTTCTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGAGAGAGCT GAATTTGAAT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCAACA CTCTGTTTCA AAACCAACAG 
TGCAGACTGG AAAAGCTGAA CAGCATGTGT ATTACAGCAG AAGGTTGTGC TGCTTTGACT 
TCAGCATTTA ACTCAAACCC TTCACACCTG AAAGAGCTCA ATCTGAGTGG GAATAAACTA 
GGAGACTCAG GAGTAAGAAA AATCTCCAGT CTACTGAGAA ACTCGCAGTG CATGCTGAAG 
ATACTCGGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTTACA TCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGGCTTACTA CTCGATCCAC ACTGTACACT GACGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAATTC TGTCAGTTTG TGTCTGGAAT TGTGGGTAAA 
TACCCATTAC TCCTGAAAGA ACTGAATCTG AGTGATCGTG GACTAGGGGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTTTAGAC TCAACACACT CATGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAA TTTAAATCCT 
TCACACCTGA TAGAGCTCAA TCTGAGTGGG AATAAACTAG AAGACTCAGG AGTAGAGAAG 
ATCTGTCCTC TGCTGGAAAA TATACAATGC CGATTAGAGA AACTGAGTCT TTCAGACTGC 
AGTATCAGGG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GATTTACTGC AGAGTAGAAG CTGTAAATTC AGGTTTCTGA AGAGTTCTGC TGCACAGGAA 
GCATGCGAGT ATCTTACTAA TGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT 
CTGAGTGAAG TTAAATTAGG AGATCTGGAC TGGGAGAAAC TCTCTGCTCT TCTGATGGAC 
TCTCATAGCA AAGTGGAGAA AATCAAGCTA AATAATTGTG AGCTGACAGA GAAAAGCTGT 
TCAGTTCTAG CTGCTGTTCT CTCCTCCAAA ACCATCCTGA AAGAGCTGAA CCTGAACAAC 
AGTCGTCTGC TGGACTCAGG AGTCAGAAAG ATCTGTGAGG GACTGAAGAA TCCTGTGTGT 
GAGCTGAAGA TACTCAATCT TTCAGACTGC AGTATTACAG AAGAAGGTTA TGAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GAGTTACTGC AGGATCCACA CTGTGCACTG 
AAGACACTGA GATTTTTAAG AAGTCCTGCT GCTGATGAAG GCTGTCAGTT AGTAACTGGA 
GTTGTGGGTA AAAACCCGTT ACTCCTGAAG GAACTGAATC TGAGTGATCA TGAGCTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGCCA GATCAACACA 
CTCAATCTGA GTTATTGCAG TATTACAGAG AAACAGTGTG AGATCCTGAC TTCAGCTCTG 
AAATCAAACC CATCACACCT GAGAGAACTG GACCTCAGTG GGAATGAACT AGGAGACTTT 
CAAAGCCTTG GCTCTCTACT GAAGAACCAA CAATTCAAGC TGGAGAAACT AATTCTGTGT 
GTTTGCAGTA TTAAAGAGAA GCAGTGTGAG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGAA CCTCAGTGGG AATGAACTCA AGCACAGAAG AGTGAAGCAA 
TTTTTTGATG TGCTGAAAAA ATCAACCTGT AAACTGGAGC GACTGAGGTT AAGATACTGT 
AGTATACAAG CCGATGATTG TACTGATCTG GCTTCAGCTC TGAAATCAAA CCCATCCCAC 
CTGAGAGAAC TGGACCTCAG TGGAAACAAT CTAGGAGACT CTGGAGTGAA AAAACTCAGT 
GATTTACTGA TAAACCAACA ATTCAAGCTG GAGAAACTAC GTCTGAGTTA TTGCAGTATT 
ACAGAGAAAC AGTGTGTGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA 
GAACTGAACC TCAATGGGAA TAGACTAGGA GACTCTGGAG CTGATGGCCT TAACCATCTG 
CTGAGCAGTG TGGATTGCAA GCTAAATAAA CTACATCTGT GTGATTGCAG TATTACAGAG 
AAACAGTTGC TCATCCCAAT CACAGCTACG TGTATGGGCC TATTACACCT GAGAGAACTG 
GACCTCAGTA AGAACCAAAT AAAAAACCAA GGAGTGTACA TGCTGTGTGA TGTGCTGAAA 
AACCCACGCT GTAGACTCAA GAGGTTAAGA CTAAATGACT GTGGCATTAC AGATGTTACA 
CCCTTAACTC AGTCTTTGAC CAAGGACAAA GCATTGAAGT TCTTAAAAGA GCTTGAACTG 
AGTAAAAATC AAATGGGATT CTTCGAAAAG CAAAAACTCA GTGACCTGCT GCGAGACTCA 
AACTGTAAAC TGAGGCTTGA GGAAGAAGAA GGTTATTTTA GAGCTGGTGT AAAACGGGTT 
ACTACATTTT TGATGCCTCC ATGGTCAAAA GTTCAAAAAC CTGTGGATCT CACAGGAGAG 
GAAAGTTCAT CATCAGATGA GGACGCCTCT GAAGATGAGA CTGATCAGGC AGAGGAGGCT 
GATAAGCTCT CAAATGAGGC GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_021469435.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325110.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X16, mRNA.

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CloneID	ODa151258
Clone ID Related Accession (Same CDS sequence)	XM_021469435.1
Accession Version	XM_021469435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	protein NLRC5 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CTGCTGATGA ATTTTGTCAG TTTGTGTCTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGAA TCTGAGTGAT CATGTACTAG CAGACACACA AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGC AAACTCAACA CACTCATTCT TTCAGACTGC 
AGTATCAGTG GAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA AGAGCTCACT 
GAGTTACTGC AGGATCCACA CTGTACACTG AAGACACTGA GGTTTTTGAG TGATGATGCA 
GATGAAGCCT GTGAACATTT GTATAAAGTT CTGGGTGGAA ACCTGTTACT CCTGAGAGAG 
CTGAATCTGA CTAAACATGA TCTTGGACCA TCAGGATTAG AGAAACTTGC TGCAGTACTG 
AAAGATAAAC ACTGTAAACT CAACACACTG ATTCTGAATA ACAGCACTAT CTCAGAGGAA 
GATTTTCATG TTCTGACTGA AGCTTTTAAT TCAAACCCAA CAAATCTGAC AGAGCTGAAC 
TTGAGTGGGA CTAAACTCAC AAACTCAGGA ATGAAGAGCT TCACAACTCT GTTTCAAAAC 
CAACAGTGTA GATTGGAAAA GCTGAAGTTC AGCAGCATGT GTATTACAGC AGAAGGTTGT 
GCTGCTTTGA CTTCAGCATT TAACTCAAAC CCTTCACACC TGAAAGAGCT CAATCTGAGT 
GGGAATAAAC TAGGAGACTC AGGAGTAAGA AAAATCTCCA GTCTACTGAG AAACTCGCAG 
TGCATGCTGA AGATACTCGG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTTA CATCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC CATCAGGAGT GAAGGAGCTC ACTGGCTTAC TACTCGATCC ACACTGTACA 
CTGACGACAC TGAGGTTTTT GAGTCCTGCT GCTGATGAAT TCTGTCAGTT TGTGTCTGGA 
ATTGTGGGTA AATACCCATT ACTCCTGAAA GAACTGAATC TGAGTGATCG TGGACTAGGG 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTTTAG ACTCAACACA 
CTCATGCTGA ATAATAACAG GATTACAGCA GAAGGTTGTG CTGCTTTAAC TTCAGCATTT 
AATTTAAATC CTTCACACCT GATAGAGCTC AATCTGAGTG GGAATAAACT AGAAGACTCA 
GGAGTAGAGA AGATCTGTCC TCTGCTGGAA AATATACAAT GCCGATTAGA GAAACTGAGT 
CTTTCAGACT GCAGTATCAG GGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACA ATCAGGAGTG 
AAGGAGCTCA CTGATTTACT GCAGAGTAGA AGCTGTAAAT TCAGGTTTCT GAAGAGTTCT 
GCTGCACAGG AAGCATGCGA GTATCTTACT AATGTTCTGG GTATAAGTCC ATTATTACTG 
ACAGAACTGG ATCTGAGTGA AGTTAAATTA GGAGATCTGG ACTGGGAGAA ACTCTCTGCT 
CTTCTGATGG ACTCTCATAG CAAAGTGGAG AAAATCAAGC TAAATAATTG TGAGCTGACA 
GAGAAAAGCT GTTCAGTTCT AGCTGCTGTT CTCTCCTCCA AAACCATCCT GAAAGAGCTG 
AACCTGAACA ACAGTCGTCT GCTGGACTCA GGAGTCAGAA AGATCTGTGA GGGACTGAAG 
AATCCTGTGT GTGAGCTGAA GATACTCAAT CTTTCAGACT GCAGTATTAC AGAAGAAGGT 
TATGAAGCTC TGGCTTCAGC TCTGAGATCA AACCCTTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAGCTCA CTGAGTTACT GCAGGATCCA 
CACTGTGCAC TGAAGACACT GAGATTTTTA AGAAGTCCTG CTGCTGATGA AGGCTGTCAG 
TTAGTAACTG GAGTTGTGGG TAAAAACCCG TTACTCCTGA AGGAACTGAA TCTGAGTGAT 
CATGAGCTAG GAGACACACG AGTGAATCAA CTCTCTGCTC TACTGCAGGA TAAACACTGC 
CAGATCAACA CACTCAATCT GAGTTATTGC AGTATTACAG AGAAACAGTG TGAGATCCTG 
ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGGACCTCAG TGGGAATGAA 
CTAGGAGACT TTCAAAGCCT TGGCTCTCTA CTGAAGAACC AACAATTCAA GCTGGAGAAA 
CTAATTCTGT GTGTTTGCAG TATTAAAGAG AAGCAGTGTG AGATCCTGAC TTCAGCTCTG 
AAATCAAACC CATCACACCT GAGAGAACTG AACCTCAGTG GGAATGAACT CAAGCACAGA 
AGAGTGAAGC AATTTTTTGA TGTGCTGAAA AAATCAACCT GTAAACTGGA GCGACTGAGG 
TTAAGATACT GTAGTATACA AGCCGATGAT TGTACTGATC TGGCTTCAGC TCTGAAATCA 
AACCCATCCC ACCTGAGAGA ACTGGACCTC AGTGGAAACA ATCTAGGAGA CTCTGGAGTG 
AAAAAACTCA GTGATTTACT GATAAACCAA CAATTCAAGC TGGAGAAACT ACGTCTGAGT 
TATTGCAGTA TTACAGAGAA ACAGTGTGTG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGAA CCTCAATGGG AATAGACTAG GAGACTCTGG AGCTGATGGC 
CTTAACCATC TGCTGAGCAG TGTGGATTGC AAGCTAAATA AACTACATCT GTGTGATTGC 
AGTATTACAG AGAAACAGTT GCTCATCCCA ATCACAGCTA CGTGTATGGG CCTATTACAC 
CTGAGAGAAC TGGACCTCAG TAAGAACCAA ATAAAAAACC AAGGAGTGTA CATGCTGTGT 
GATGTGCTGA AAAACCCACG CTGTAGACTC AAGAGGTTAA GACTAAATGA CTGTGGCATT 
ACAGATGTTA CACCCTTAAC TCAGTCTTTG ACCAAGGACA AAGCATTGAA GTTCTTAAAA 
GAGCTTGAAC TGAGTAAAAA TCAAATGGGA TTCTTCGAAA AGCAAAAACT CAGTGACCTG 
CTGCGAGACT CAAACTGTAA ACTGAGGCTT GAGGAAGAAG AAGGTTATTT TAGAGCTGGT 
GTAAAACGGG TTACTACATT TTTGATGCCT CCATGGTCAA AAGTTCAAAA ACCTGTGGAT 
CTCACAGGAG AGGAAAGTTC ATCATCAGAT GAGGACGCCT CTGAAGATGA GACTGATCAG 
GCAGAGGAGG CTGATAAGCT CTCAAATGAG GCGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325110.1
CDS	65..5503
Translation	MSNNNLQDSGVKLLCTALKNVKCELEVLRISYCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGILGKNPLLLKELNLSDCELGDTGVNQLSALLQDKHCKPKTLMLRGCGLKEETCSALATVLGSDTSLKELDMSNNNLQDLGVKKLQSGLENTNCTLEKLSLSDCNISEEGYKALASALRSNPSHLIELNLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAAEEFCQFVTGIVGKNPLLLKELYLSDHELGDTRVNQLSALLQDKHCTVSTLILHNCGLTKESCSALASALSSDTSLKELDLCNNNLQDSGVKKLQSELQNTNWRLKKLRLSKCDLTEESCSALASVLTSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSKLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELIELLQDPLCTLKTLRFLSPAADEACQFVTGIVGKNLLLLKELNLSDRQLGDTRVNQLSALLQDKHCTLNTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACEHLYKVLGGNLLLLRELNLTKHDLGPSGLEKLAAVLKDKHCKLNTLILNNSTISEEDFHVLTEAFNSNPTNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_021469435.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469435.1

ORF Insert Sequence:

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CTGCTGATGA ATTTTGTCAG TTTGTGTCTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGAA TCTGAGTGAT CATGTACTAG CAGACACACA AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGC AAACTCAACA CACTCATTCT TTCAGACTGC 
AGTATCAGTG GAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA AGAGCTCACT 
GAGTTACTGC AGGATCCACA CTGTACACTG AAGACACTGA GGTTTTTGAG TGATGATGCA 
GATGAAGCCT GTGAACATTT GTATAAAGTT CTGGGTGGAA ACCTGTTACT CCTGAGAGAG 
CTGAATCTGA CTAAACATGA TCTTGGACCA TCAGGATTAG AGAAACTTGC TGCAGTACTG 
AAAGATAAAC ACTGTAAACT CAACACACTG ATTCTGAATA ACAGCACTAT CTCAGAGGAA 
GATTTTCATG TTCTGACTGA AGCTTTTAAT TCAAACCCAA CAAATCTGAC AGAGCTGAAC 
TTGAGTGGGA CTAAACTCAC AAACTCAGGA ATGAAGAGCT TCACAACTCT GTTTCAAAAC 
CAACAGTGTA GATTGGAAAA GCTGAAGTTC AGCAGCATGT GTATTACAGC AGAAGGTTGT 
GCTGCTTTGA CTTCAGCATT TAACTCAAAC CCTTCACACC TGAAAGAGCT CAATCTGAGT 
GGGAATAAAC TAGGAGACTC AGGAGTAAGA AAAATCTCCA GTCTACTGAG AAACTCGCAG 
TGCATGCTGA AGATACTCGG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTTA CATCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC CATCAGGAGT GAAGGAGCTC ACTGGCTTAC TACTCGATCC ACACTGTACA 
CTGACGACAC TGAGGTTTTT GAGTCCTGCT GCTGATGAAT TCTGTCAGTT TGTGTCTGGA 
ATTGTGGGTA AATACCCATT ACTCCTGAAA GAACTGAATC TGAGTGATCG TGGACTAGGG 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTTTAG ACTCAACACA 
CTCATGCTGA ATAATAACAG GATTACAGCA GAAGGTTGTG CTGCTTTAAC TTCAGCATTT 
AATTTAAATC CTTCACACCT GATAGAGCTC AATCTGAGTG GGAATAAACT AGAAGACTCA 
GGAGTAGAGA AGATCTGTCC TCTGCTGGAA AATATACAAT GCCGATTAGA GAAACTGAGT 
CTTTCAGACT GCAGTATCAG GGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACA ATCAGGAGTG 
AAGGAGCTCA CTGATTTACT GCAGAGTAGA AGCTGTAAAT TCAGGTTTCT GAAGAGTTCT 
GCTGCACAGG AAGCATGCGA GTATCTTACT AATGTTCTGG GTATAAGTCC ATTATTACTG 
ACAGAACTGG ATCTGAGTGA AGTTAAATTA GGAGATCTGG ACTGGGAGAA ACTCTCTGCT 
CTTCTGATGG ACTCTCATAG CAAAGTGGAG AAAATCAAGC TAAATAATTG TGAGCTGACA 
GAGAAAAGCT GTTCAGTTCT AGCTGCTGTT CTCTCCTCCA AAACCATCCT GAAAGAGCTG 
AACCTGAACA ACAGTCGTCT GCTGGACTCA GGAGTCAGAA AGATCTGTGA GGGACTGAAG 
AATCCTGTGT GTGAGCTGAA GATACTCAAT CTTTCAGACT GCAGTATTAC AGAAGAAGGT 
TATGAAGCTC TGGCTTCAGC TCTGAGATCA AACCCTTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAGCTCA CTGAGTTACT GCAGGATCCA 
CACTGTGCAC TGAAGACACT GAGATTTTTA AGAAGTCCTG CTGCTGATGA AGGCTGTCAG 
TTAGTAACTG GAGTTGTGGG TAAAAACCCG TTACTCCTGA AGGAACTGAA TCTGAGTGAT 
CATGAGCTAG GAGACACACG AGTGAATCAA CTCTCTGCTC TACTGCAGGA TAAACACTGC 
CAGATCAACA CACTCAATCT GAGTTATTGC AGTATTACAG AGAAACAGTG TGAGATCCTG 
ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGGACCTCAG TGGGAATGAA 
CTAGGAGACT TTCAAAGCCT TGGCTCTCTA CTGAAGAACC AACAATTCAA GCTGGAGAAA 
CTAATTCTGT GTGTTTGCAG TATTAAAGAG AAGCAGTGTG AGATCCTGAC TTCAGCTCTG 
AAATCAAACC CATCACACCT GAGAGAACTG AACCTCAGTG GGAATGAACT CAAGCACAGA 
AGAGTGAAGC AATTTTTTGA TGTGCTGAAA AAATCAACCT GTAAACTGGA GCGACTGAGG 
TTAAGATACT GTAGTATACA AGCCGATGAT TGTACTGATC TGGCTTCAGC TCTGAAATCA 
AACCCATCCC ACCTGAGAGA ACTGGACCTC AGTGGAAACA ATCTAGGAGA CTCTGGAGTG 
AAAAAACTCA GTGATTTACT GATAAACCAA CAATTCAAGC TGGAGAAACT ACGTCTGAGT 
TATTGCAGTA TTACAGAGAA ACAGTGTGTG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGAA CCTCAATGGG AATAGACTAG GAGACTCTGG AGCTGATGGC 
CTTAACCATC TGCTGAGCAG TGTGGATTGC AAGCTAAATA AACTACATCT GTGTGATTGC 
AGTATTACAG AGAAACAGTT GCTCATCCCA ATCACAGCTA CGTGTATGGG CCTATTACAC 
CTGAGAGAAC TGGACCTCAG TAAGAACCAA ATAAAAAACC AAGGAGTGTA CATGCTGTGT 
GATGTGCTGA AAAACCCACG CTGTAGACTC AAGAGGTTAA GACTAAATGA CTGTGGCATT 
ACAGATGTTA CACCCTTAAC TCAGTCTTTG ACCAAGGACA AAGCATTGAA GTTCTTAAAA 
GAGCTTGAAC TGAGTAAAAA TCAAATGGGA TTCTTCGAAA AGCAAAAACT CAGTGACCTG 
CTGCGAGACT CAAACTGTAA ACTGAGGCTT GAGGAAGAAG AAGGTTATTT TAGAGCTGGT 
GTAAAACGGG TTACTACATT TTTGATGCCT CCATGGTCAA AAGTTCAAAA ACCTGTGGAT 
CTCACAGGAG AGGAAAGTTC ATCATCAGAT GAGGACGCCT CTGAAGATGA GACTGATCAG 
GCAGAGGAGG CTGATAAGCT CTCAAATGAG GCGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_021469428.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325103.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X8, mRNA.

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CloneID	ODa151251
Clone ID Related Accession (Same CDS sequence)	XM_021469428.1
Accession Version	XM_021469428.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein si:ch211-11p18.6 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGTCTT 
TCAGACTGCA GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG CTTTGATTCC AGAGTGTGCT CTGAAAACAT TGAGGTTTTT GGGTCCTGCA 
GCAGAAGAAG CCTGTCAGTT TGTGACTGGA ATTGTGGGTA AAAACCCGTT ACTCCTGAAA 
GAACTGAATC TGAGTGATCG TAAACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTA 
CTGCAGGATA AACACTGTAA ACCCAACACA CTTCAGCTGA ATAATAACAG GATTACAGCA 
GAAGGTTGTG CTGCTTTAAC TTCAGCATTT AATTTAAACC CTTCACACCT AATAGAGCTC 
AATCTGAGTG GGAATAAACT AAGAGACTCA GGAGTAGAGG AGATCTGTCC TCTGCTGAAA 
AATACACAGT GCAGACTGGA GAGACTAAGT CTTTCAGACT GCAGTATCAG TGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGAGATCA AACCCTTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTA AAGGAGCTCA CTGACTTACT GCAGGATGAG 
CGATATAGAT TGAAGACCCT CAGGCTTTTG AAAACTACTG CAGCACAAGA AGCGTGTGAC 
TATCTTACTA AAGTTGTGGG TAAAAGTCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA CTCTCTGCTC TTCTGATGGA CTCTCATAGT 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCAGATTG AAAAAGGCTG TTCAGTACTA 
GCTGCCGTTC TCTCCTCAAA AACAATCCTA AAAGAGATGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCTTAGA GATCTGTGAG GGACTGAAGA ACCCTGTGTG TGAGCTGAAG 
ATTCTCAAGC TCAGTAAGTG TGATTTAACA GAGGAAAGCT GTTCAGCTCT GGCTTCAGTT 
CTCAGATCAG ACTCCAGCAG TCTGAAGGAT CTTGACCTGA GCAATAATAA TCTGCAGGAT 
TCAGGAGTGA AGTTGCTCTC TGATGGACTA AAGCACAGTA AACTGAAGAG ACTCAGTCTT 
TCAGACTGCA GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG ACTTACTGCA GGATCCACAC TGTACACTAA AGACACTGAG TTGTTTTAGT 
CCTGCTGCTG ATGAAGGCTG TCAGTTTGTT ACTGGAATTG TGGGTAAAAA CCCGTTACTA 
CTGAAAGAAC TGAATCTGAG TGATCGTGAG CTAGGAGACA CACGGGTGAA TCAACTCTCT 
GCTCTACTGC AAGATAAACA CTGTACACTC AATATACTCA CGCTGAATAA TAACAGGATT 
ACAGCAGAAG GTTGTGCTGC TTTGACTTCA GCATTTAATT TAAACCCTTC ACACCTGATA 
GAGCTCAATC TGAGTGGAAA TAAACTAGGA GACTCAGGAG TAGAGAAGAT CTGTCCTCTG 
CTGAAAAATA CCCAGTGCAG ATTGGAGAAA TTAAATCTTT CAGACTGCAG CATCACTGGA 
AAAGGTTATG AAACATTGAT TTCAGCTTTG AAGTCAAACT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG GAAATCAGGA GTGCAGAAGC TCACTGATTT GCTACGAGCT 
GGAAGCTGTA AATTAAAAAC CATCAGGACT TTGAAAGGTT CCGCTGCACA GGAAGCGTGT 
GACTATCTAA CTAAAGCTGT GGGTAAAAGT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAACTCTCTG CTCTTCTGAT GGACTCTCAT 
AGCAAAGTGG AGAAAATCAG GATGAATAAC TGTAAAAAGC TGACAGAGAA AAGCTGTTTA 
GTTCTAGCTG CTGTTCTTTC ATCCAAAACC ATCTTGAAAG AGATGAACCT AAACAACAGT 
CGTCTGCTGG ACTCAGGAGT CAAAGTGATC TGTGAGGGAC TGAAGAACTC TAAGCTGGAG 
ATTTTAAAAC TTTCAAATTG TAGTATTACA GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCTGGAGTAA AGGAGCTCAC TGAGTTACTG CAGAGTTCGT GGATTCCATG GAAAAAAGTG 
AAGTGTTCAA TGAGGTTTTT GAGTCCTGCT GCTGATGAAT TTTGTCAGTT TGTGTCTGGA 
ATTGTGGGTA AAAACCCGTT ACTCCTGAAA GAACTGAATC TGAGTGATCA TGTACTAGCA 
GACACACAAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGCAA ACTCAACACA 
CTCATTCTTT CAGACTGCAG TATCAGTGGA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAAG AGCTCACTGA GTTACTGCAG GATCCACACT GTACACTGAA GACACTGAGG 
TTTTTGAGTG ATGATGCAGA TGAAGCCTGT GAACATTTGT ATAAAGTTCT GGGTGGAAAC 
CTGTTACTCC TGAGAGAGCT GAATCTGACT AAACATGATC TTGGACCATC AGGATTAGAG 
AAACTTGCTG CAGTACTGAA AGATAAACAC TGTAAACTCA ACACACTGAT TCTGAATAAC 
AGCACTATCT CAGAGGAAGA TTTTCATGTT CTGACTGAAG CTTTTAATTC AAACCCAACA 
AATCTGACAG AGCTGAACTT GAGTGGGACT AAACTCACAA ACTCAGGAAT GAAGAGCTTC 
ACAACTCTGT TTCAAAACCA ACAGTGTAGA TTGGAAAAGC TGAAGTTCAG CAGCATGTGT 
ATTACAGCAG AAGGTTGTGC TGCTTTGACT TCAGCATTTA ACTCAAACCC TTCACACCTG 
AAAGAGCTCA ATCTGAGTGG GAATAAACTA GGAGACTCAG GAGTAAGAAA AATCTCCAGT 
CTACTGAGAA ACTCGCAGTG CATGCTGAAG ATACTCGGTC TTTCAGACTG CAGTATCAGT 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTTACA TCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACCA TCAGGAGTGA AGGAGCTCAC TGGCTTACTA 
CTCGATCCAC ACTGTACACT GACGACACTG AGGTTTTTGA GTCCTGCTGC TGATGAATTC 
TGTCAGTTTG TGTCTGGAAT TGTGGGTAAA TACCCATTAC TCCTGAAAGA ACTGAATCTG 
AGTGATCGTG GACTAGGGGA CACACGAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA 
CACTTTAGAC TCAACACACT CATGCTGAAT AATAACAGGA TTACAGCAGA AGGTTGTGCT 
GCTTTAACTT CAGCATTTAA TTTAAATCCT TCACACCTGA TAGAGCTCAA TCTGAGTGGG 
AATAAACTAG AAGACTCAGG AGTAGAGAAG ATCTGTCCTC TGCTGGAAAA TATACAATGC 
CGATTAGAGA AACTGAGTCT TTCAGACTGC AGTATCAGGG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GATTTACTGC AGAGTAGAAG CTGTAAATTC 
AGGTTTCTGA AGAGTTCTGC TGCACAGGAA GCATGCGAGT ATCTTACTAA TGTTCTGGGT 
ATAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG TTAAATTAGG AGATCTGGAC 
TGGGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGCA AAGTGGAGAA AATCAAGCTA 
AATAATTGTG AGCTGACAGA GAAAAGCTGT TCAGTTCTAG CTGCTGTTCT CTCCTCCAAA 
ACCATCCTGA AAGAGCTGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCAGAAAG 
ATCTGTGAGG GACTGAAGAA TCCTGTGTGT GAGCTGAAGA TACTCAATCT TTCAGACTGC 
AGTATTACAG AAGAAGGTTA TGAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GAGTTACTGC AGGATCCACA CTGTGCACTG AAGACACTGA GATTTTTAAG AAGTCCTGCT 
GCTGATGAAG GCTGTCAGTT AGTAACTGGA GTTGTGGGTA AAAACCCGTT ACTCCTGAAG 
GAACTGAATC TGAGTGATCA TGAGCTAGGA GACACACGAG TGAATCAACT CTCTGCTCTA 
CTGCAGGATA AACACTGCCA GATCAACACA CTCAATCTGA GTTATTGCAG TATTACAGAG 
AAACAGTGTG AGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
GACCTCAGTG GGAATGAACT AGGAGACTTT CAAAGCCTTG GCTCTCTACT GAAGAACCAA 
CAATTCAAGC TGGAGAAACT AATTCTGTGT GTTTGCAGTA TTAAAGAGAA GCAGTGTGAG 
ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAGTGGG 
AATGAACTCA AGCACAGAAG AGTGAAGCAA TTTTTTGATG TGCTGAAAAA ATCAACCTGT 
AAACTGGAGC GACTGAGGTT AAGATACTGT AGTATACAAG CCGATGATTG TACTGATCTG 
GCTTCAGCTC TGAAATCAAA CCCATCCCAC CTGAGAGAAC TGGACCTCAG TGGAAACAAT 
CTAGGAGACT CTGGAGTGAA AAAACTCAGT GATTTACTGA TAAACCAACA ATTCAAGCTG 
GAGAAACTAC GTCTGAGTTA TTGCAGTATT ACAGAGAAAC AGTGTGTGAT CCTGACTTCA 
GCTCTGAAAT CAAACCCATC ACACCTGAGA GAACTGAACC TCAATGGGAA TAGACTAGGA 
GACTCTGGAG CTGATGGCCT TAACCATCTG CTGAGCAGTG TGGATTGCAA GCTAAATAAA 
CTACATCTGT GTGATTGCAG TATTACAGAG AAACAGTTGC TCATCCCAAT CACAGCTACG 
TGTATGGGCC TATTACACCT GAGAGAACTG GACCTCAGTA AGAACCAAAT AAAAAACCAA 
GGAGTGTACA TGCTGTGTGA TGTGCTGAAA AACCCACGCT GTAGACTCAA GAGGTTAAGA 
CTAAATGACT GTGGCATTAC AGATGTTACA CCCTTAACTC AGTCTTTGAC CAAGGACAAA 
GCATTGAAGT TCTTAAAAGA GCTTGAACTG AGTAAAAATC AAATGGGATT CTTCGAAAAG 
CAAAAACTCA GTGACCTGCT GCGAGACTCA AACTGTAAAC TGAGGCTTGA GGAAGAAGAA 
GGTTATTTTA GAGCTGGTGT AAAACGGGTT ACTACATTTT TGATGCCTCC ATGGTCAAAA 
GTTCAAAAAC CTGTGGATCT CACAGGAGAG GAAAGTTCAT CATCAGATGA GGACGCCTCT 
GAAGATGAGA CTGATCAGGC AGAGGAGGCT GATAAGCTCT CAAATGAGGC GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325103.1
CDS	65..7921
Translation	MSNNNLQDSGVKLLCTALKNVKCELEVLRISYCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGILGKNPLLLKELNLSDCELGDTGVNQLSALLQDKHCKPKTLMLRGCGLKEETCSALATVLGSDTSLKELDMSNNNLQDLGVKKLQSGLENTNCTLEKLSLSDCNISEEGYKALASALRSNPSHLIELNLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAAEEFCQFVTGIVGKNPLLLKELYLSDHELGDTRVNQLSALLQDKHCTVSTLILHNCGLTKESCSALASALSSDTSLKELDLCNNNLQDSGVKKLQSELQNTNWRLKKLRLSKCDLTEESCSALASVLTSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSKLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELIELLQDPLCTLKTLRFLSPAADEACQFVTGIVGKNLLLLKELNLSDRQLGDTRVNQLSALLQDKHCTLNTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACEHLYKVLGGNLLLLRELNLTKHDLGPSGLEKLAAVLKDKHCKLNTLILNNSTISEEDFHVLTEAFNSNPTNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_021469428.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469428.1

ORF Insert Sequence:

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGTCTT 
TCAGACTGCA GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG CTTTGATTCC AGAGTGTGCT CTGAAAACAT TGAGGTTTTT GGGTCCTGCA 
GCAGAAGAAG CCTGTCAGTT TGTGACTGGA ATTGTGGGTA AAAACCCGTT ACTCCTGAAA 
GAACTGAATC TGAGTGATCG TAAACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTA 
CTGCAGGATA AACACTGTAA ACCCAACACA CTTCAGCTGA ATAATAACAG GATTACAGCA 
GAAGGTTGTG CTGCTTTAAC TTCAGCATTT AATTTAAACC CTTCACACCT AATAGAGCTC 
AATCTGAGTG GGAATAAACT AAGAGACTCA GGAGTAGAGG AGATCTGTCC TCTGCTGAAA 
AATACACAGT GCAGACTGGA GAGACTAAGT CTTTCAGACT GCAGTATCAG TGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGAGATCA AACCCTTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTA AAGGAGCTCA CTGACTTACT GCAGGATGAG 
CGATATAGAT TGAAGACCCT CAGGCTTTTG AAAACTACTG CAGCACAAGA AGCGTGTGAC 
TATCTTACTA AAGTTGTGGG TAAAAGTCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA CTCTCTGCTC TTCTGATGGA CTCTCATAGT 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCAGATTG AAAAAGGCTG TTCAGTACTA 
GCTGCCGTTC TCTCCTCAAA AACAATCCTA AAAGAGATGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCTTAGA GATCTGTGAG GGACTGAAGA ACCCTGTGTG TGAGCTGAAG 
ATTCTCAAGC TCAGTAAGTG TGATTTAACA GAGGAAAGCT GTTCAGCTCT GGCTTCAGTT 
CTCAGATCAG ACTCCAGCAG TCTGAAGGAT CTTGACCTGA GCAATAATAA TCTGCAGGAT 
TCAGGAGTGA AGTTGCTCTC TGATGGACTA AAGCACAGTA AACTGAAGAG ACTCAGTCTT 
TCAGACTGCA GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG ACTTACTGCA GGATCCACAC TGTACACTAA AGACACTGAG TTGTTTTAGT 
CCTGCTGCTG ATGAAGGCTG TCAGTTTGTT ACTGGAATTG TGGGTAAAAA CCCGTTACTA 
CTGAAAGAAC TGAATCTGAG TGATCGTGAG CTAGGAGACA CACGGGTGAA TCAACTCTCT 
GCTCTACTGC AAGATAAACA CTGTACACTC AATATACTCA CGCTGAATAA TAACAGGATT 
ACAGCAGAAG GTTGTGCTGC TTTGACTTCA GCATTTAATT TAAACCCTTC ACACCTGATA 
GAGCTCAATC TGAGTGGAAA TAAACTAGGA GACTCAGGAG TAGAGAAGAT CTGTCCTCTG 
CTGAAAAATA CCCAGTGCAG ATTGGAGAAA TTAAATCTTT CAGACTGCAG CATCACTGGA 
AAAGGTTATG AAACATTGAT TTCAGCTTTG AAGTCAAACT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG GAAATCAGGA GTGCAGAAGC TCACTGATTT GCTACGAGCT 
GGAAGCTGTA AATTAAAAAC CATCAGGACT TTGAAAGGTT CCGCTGCACA GGAAGCGTGT 
GACTATCTAA CTAAAGCTGT GGGTAAAAGT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAACTCTCTG CTCTTCTGAT GGACTCTCAT 
AGCAAAGTGG AGAAAATCAG GATGAATAAC TGTAAAAAGC TGACAGAGAA AAGCTGTTTA 
GTTCTAGCTG CTGTTCTTTC ATCCAAAACC ATCTTGAAAG AGATGAACCT AAACAACAGT 
CGTCTGCTGG ACTCAGGAGT CAAAGTGATC TGTGAGGGAC TGAAGAACTC TAAGCTGGAG 
ATTTTAAAAC TTTCAAATTG TAGTATTACA GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCTGGAGTAA AGGAGCTCAC TGAGTTACTG CAGAGTTCGT GGATTCCATG GAAAAAAGTG 
AAGTGTTCAA TGAGGTTTTT GAGTCCTGCT GCTGATGAAT TTTGTCAGTT TGTGTCTGGA 
ATTGTGGGTA AAAACCCGTT ACTCCTGAAA GAACTGAATC TGAGTGATCA TGTACTAGCA 
GACACACAAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGCAA ACTCAACACA 
CTCATTCTTT CAGACTGCAG TATCAGTGGA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAAG AGCTCACTGA GTTACTGCAG GATCCACACT GTACACTGAA GACACTGAGG 
TTTTTGAGTG ATGATGCAGA TGAAGCCTGT GAACATTTGT ATAAAGTTCT GGGTGGAAAC 
CTGTTACTCC TGAGAGAGCT GAATCTGACT AAACATGATC TTGGACCATC AGGATTAGAG 
AAACTTGCTG CAGTACTGAA AGATAAACAC TGTAAACTCA ACACACTGAT TCTGAATAAC 
AGCACTATCT CAGAGGAAGA TTTTCATGTT CTGACTGAAG CTTTTAATTC AAACCCAACA 
AATCTGACAG AGCTGAACTT GAGTGGGACT AAACTCACAA ACTCAGGAAT GAAGAGCTTC 
ACAACTCTGT TTCAAAACCA ACAGTGTAGA TTGGAAAAGC TGAAGTTCAG CAGCATGTGT 
ATTACAGCAG AAGGTTGTGC TGCTTTGACT TCAGCATTTA ACTCAAACCC TTCACACCTG 
AAAGAGCTCA ATCTGAGTGG GAATAAACTA GGAGACTCAG GAGTAAGAAA AATCTCCAGT 
CTACTGAGAA ACTCGCAGTG CATGCTGAAG ATACTCGGTC TTTCAGACTG CAGTATCAGT 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTTACA TCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACCA TCAGGAGTGA AGGAGCTCAC TGGCTTACTA 
CTCGATCCAC ACTGTACACT GACGACACTG AGGTTTTTGA GTCCTGCTGC TGATGAATTC 
TGTCAGTTTG TGTCTGGAAT TGTGGGTAAA TACCCATTAC TCCTGAAAGA ACTGAATCTG 
AGTGATCGTG GACTAGGGGA CACACGAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA 
CACTTTAGAC TCAACACACT CATGCTGAAT AATAACAGGA TTACAGCAGA AGGTTGTGCT 
GCTTTAACTT CAGCATTTAA TTTAAATCCT TCACACCTGA TAGAGCTCAA TCTGAGTGGG 
AATAAACTAG AAGACTCAGG AGTAGAGAAG ATCTGTCCTC TGCTGGAAAA TATACAATGC 
CGATTAGAGA AACTGAGTCT TTCAGACTGC AGTATCAGGG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GATTTACTGC AGAGTAGAAG CTGTAAATTC 
AGGTTTCTGA AGAGTTCTGC TGCACAGGAA GCATGCGAGT ATCTTACTAA TGTTCTGGGT 
ATAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG TTAAATTAGG AGATCTGGAC 
TGGGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGCA AAGTGGAGAA AATCAAGCTA 
AATAATTGTG AGCTGACAGA GAAAAGCTGT TCAGTTCTAG CTGCTGTTCT CTCCTCCAAA 
ACCATCCTGA AAGAGCTGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCAGAAAG 
ATCTGTGAGG GACTGAAGAA TCCTGTGTGT GAGCTGAAGA TACTCAATCT TTCAGACTGC 
AGTATTACAG AAGAAGGTTA TGAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GAGTTACTGC AGGATCCACA CTGTGCACTG AAGACACTGA GATTTTTAAG AAGTCCTGCT 
GCTGATGAAG GCTGTCAGTT AGTAACTGGA GTTGTGGGTA AAAACCCGTT ACTCCTGAAG 
GAACTGAATC TGAGTGATCA TGAGCTAGGA GACACACGAG TGAATCAACT CTCTGCTCTA 
CTGCAGGATA AACACTGCCA GATCAACACA CTCAATCTGA GTTATTGCAG TATTACAGAG 
AAACAGTGTG AGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
GACCTCAGTG GGAATGAACT AGGAGACTTT CAAAGCCTTG GCTCTCTACT GAAGAACCAA 
CAATTCAAGC TGGAGAAACT AATTCTGTGT GTTTGCAGTA TTAAAGAGAA GCAGTGTGAG 
ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAGTGGG 
AATGAACTCA AGCACAGAAG AGTGAAGCAA TTTTTTGATG TGCTGAAAAA ATCAACCTGT 
AAACTGGAGC GACTGAGGTT AAGATACTGT AGTATACAAG CCGATGATTG TACTGATCTG 
GCTTCAGCTC TGAAATCAAA CCCATCCCAC CTGAGAGAAC TGGACCTCAG TGGAAACAAT 
CTAGGAGACT CTGGAGTGAA AAAACTCAGT GATTTACTGA TAAACCAACA ATTCAAGCTG 
GAGAAACTAC GTCTGAGTTA TTGCAGTATT ACAGAGAAAC AGTGTGTGAT CCTGACTTCA 
GCTCTGAAAT CAAACCCATC ACACCTGAGA GAACTGAACC TCAATGGGAA TAGACTAGGA 
GACTCTGGAG CTGATGGCCT TAACCATCTG CTGAGCAGTG TGGATTGCAA GCTAAATAAA 
CTACATCTGT GTGATTGCAG TATTACAGAG AAACAGTTGC TCATCCCAAT CACAGCTACG 
TGTATGGGCC TATTACACCT GAGAGAACTG GACCTCAGTA AGAACCAAAT AAAAAACCAA 
GGAGTGTACA TGCTGTGTGA TGTGCTGAAA AACCCACGCT GTAGACTCAA GAGGTTAAGA 
CTAAATGACT GTGGCATTAC AGATGTTACA CCCTTAACTC AGTCTTTGAC CAAGGACAAA 
GCATTGAAGT TCTTAAAAGA GCTTGAACTG AGTAAAAATC AAATGGGATT CTTCGAAAAG 
CAAAAACTCA GTGACCTGCT GCGAGACTCA AACTGTAAAC TGAGGCTTGA GGAAGAAGAA 
GGTTATTTTA GAGCTGGTGT AAAACGGGTT ACTACATTTT TGATGCCTCC ATGGTCAAAA 
GTTCAAAAAC CTGTGGATCT CACAGGAGAG GAAAGTTCAT CATCAGATGA GGACGCCTCT 
GAAGATGAGA CTGATCAGGC AGAGGAGGCT GATAAGCTCT CAAATGAGGC GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_021469429.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325104.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X9, mRNA.

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CloneID	ODa151252
Clone ID Related Accession (Same CDS sequence)	XM_021469429.1
Accession Version	XM_021469429.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein si:ch211-11p18.6 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACAACGGGGT GATTGCTGTA AATTAAAAAC CGTCAGGTTT 
TTAAAGAGTT CTGCTGCACA GGAAGCGTGT GACTATCTTA CTAAAGCTGT GGGTAAAAGT 
CCATTATTAC TGACAAAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAA GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTGAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAGATCTGT 
GAGGGGCTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ACCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACTTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGAAGCT CACTGACTTA 
CTGCAGGATC CACACTGTAC CCTGAATACA CTGAGGTTTT TGAGTCCTGC CGCAGAAGAA 
GCCTGTCAGT TTGTGACTGG AATTGTGGGT AAAAACCCGA TACTTCTGAA AGAACTGAAT 
CTGAGTGATC ATGAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AAAAACAGCT TTATCTCAGA GGAAGATTGT 
CGTGTTCTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGAGAGAGCT GAATTTGAAT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCAACA CTCTGTTTCA AAACCAACAG 
TGCAGACTGG AAAAGCTGAA GTTTAGCGGC ATCAGTATCA CAGCAGAAGG TTGTGCTGCT 
TTGACTTCAG CATTTAGCTC AAACCCGTCA CACCTGATAG AGCTAAATCT GAGTGGGAAT 
ACACTAGAAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC ACAGTGCATG 
CTGAAGATAC TCAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGCT TTGATTCCAG AGTGTGCTCT GAAAACATTG 
AGGTTTTTGG GTCCTGCAGC AGAAGAAGCC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCGTTAC TCCTGAAAGA ACTGAATCTG AGTGATCGTA AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC CCAACACACT TCAGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAA TTTAAACCCT 
TCACACCTAA TAGAGCTCAA TCTGAGTGGG AATAAACTAA GAGACTCAGG AGTAGAGGAG 
ATCTGTCCTC TGCTGAAAAA TACACAGTGC AGACTGGAGA GACTAAGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTAAA GGAGCTCACT 
GACTTACTGC AGGATGAGCG ATATAGATTG AAGACCCTCA GGCTTTTGAA AACTACTGCA 
GCACAAGAAG CGTGTGACTA TCTTACTAAA GTTGTGGGTA AAAGTCCATT ATTACTGACA 
GAACTGGATC TGAGTGAAGA TAAATTAGGA GATCTGGATG GAGAGAAACT CTCTGCTCTT 
CTGATGGACT CTCATAGTAA AGTGGAGAAA ATCAAGCTGA ATAATTGTAA GCAGATTGAA 
AAAGGCTGTT CAGTACTAGC TGCCGTTCTC TCCTCAAAAA CAATCCTAAA AGAGATGAAC 
CTGAACAACA GTCGTCTGCT GGACTCAGGA GTCTTAGAGA TCTGTGAGGG ACTGAAGAAC 
CCTGTGTGTG AGCTGAAGAT TCTCAAGCTC AGTAAGTGTG ATTTAACAGA GGAAAGCTGT 
TCAGCTCTGG CTTCAGTTCT CAGATCAGAC TCCAGCAGTC TGAAGGATCT TGACCTGAGC 
AATAATAATC TGCAGGATTC AGGAGTGAAG TTGCTCTCTG ATGGACTAAA GCACAGTAAA 
CTGAAGAGAC TCAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAC TTACTGCAGG ATCCACACTG TACACTAAAG 
ACACTGAGTT GTTTTAGTCC TGCTGCTGAT GAAGGCTGTC AGTTTGTTAC TGGAATTGTG 
GGTAAAAACC CGTTACTACT GAAAGAACTG AATCTGAGTG ATCGTGAGCT AGGAGACACA 
CGGGTGAATC AACTCTCTGC TCTACTGCAA GATAAACACT GTACACTCAA TATACTCACG 
CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAATTTA 
AACCCTTCAC ACCTGATAGA GCTCAATCTG AGTGGAAATA AACTAGGAGA CTCAGGAGTA 
GAGAAGATCT GTCCTCTGCT GAAAAATACC CAGTGCAGAT TGGAGAAATT AAATCTTTCA 
GACTGCAGCA TCACTGGAAA AGGTTATGAA ACATTGATTT CAGCTTTGAA GTCAAACTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGGA AATCAGGAGT GCAGAAGCTC 
ACTGATTTGC TACGAGCTGG AAGCTGTAAA TTAAAAACCA TCAGGACTTT GAAAGGTTCC 
GCTGCACAGG AAGCGTGTGA CTATCTAACT AAAGCTGTGG GTAAAAGTCC ATTATTACTG 
ACAGAACTGG ATCTGAGTGA AGATAAATTA GGAGATCTGG ATGGAGAGAA ACTCTCTGCT 
CTTCTGATGG ACTCTCATAG CAAAGTGGAG AAAATCAGGA TGAATAACTG TAAAAAGCTG 
ACAGAGAAAA GCTGTTTAGT TCTAGCTGCT GTTCTTTCAT CCAAAACCAT CTTGAAAGAG 
ATGAACCTAA ACAACAGTCG TCTGCTGGAC TCAGGAGTCA AAGTGATCTG TGAGGGACTG 
AAGAACTCTA AGCTGGAGAT TTTAAAACTT TCAAATTGTA GTATTACAGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC TGGAGTAAAG GAGCTCACTG AGTTACTGCA GAGTTCGTGG 
ATTCCATGGA AAAAAGTGAA GTGTTCAATG AGGTTTTTGA GTCCTGCTGC TGATGAATTT 
TGTCAGTTTG TGTCTGGAAT TGTGGGTAAA AACCCGTTAC TCCTGAAAGA ACTGAATCTG 
AGTGATCATG TACTAGCAGA CACACAAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA 
CACTGCAAAC TCAACACACT CATGCTGAAT AATAACAGGA TTACAGCAGA AGGTTGTGCT 
GCTTTAACTT CAGCATTTAA TTTAAATCCT TCACACCTGA TAGAGCTCAA TCTGAGTGGG 
AATAAACTAG AAGACTCAGG AGTAGAGAAG ATCTGTCCTC TGCTGGAAAA TATACAATGC 
CGATTAGAGA AACTGAGTCT TTCAGACTGC AGTATCAGGG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GATTTACTGC AGAGTAGAAG CTGTAAATTC 
AGGTTTCTGA AGAGTTCTGC TGCACAGGAA GCATGCGAGT ATCTTACTAA TGTTCTGGGT 
ATAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG TTAAATTAGG AGATCTGGAC 
TGGGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGCA AAGTGGAGAA AATCAAGCTA 
AATAATTGTG AGCTGACAGA GAAAAGCTGT TCAGTTCTAG CTGCTGTTCT CTCCTCCAAA 
ACCATCCTGA AAGAGCTGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCAGAAAG 
ATCTGTGAGG GACTGAAGAA TCCTGTGTGT GAGCTGAAGA TACTCAATCT TTCAGACTGC 
AGTATTACAG AAGAAGGTTA TGAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GAGTTACTGC AGGATCCACA CTGTGCACTG AAGACACTGA GATTTTTAAG AAGTCCTGCT 
GCTGATGAAG GCTGTCAGTT AGTAACTGGA GTTGTGGGTA AAAACCCGTT ACTCCTGAAG 
GAACTGAATC TGAGTGATCA TGAGCTAGGA GACACACGAG TGAATCAACT CTCTGCTCTA 
CTGCAGGATA AACACTGCCA GATCAACACA CTCAATCTGA GTTATTGCAG TATTACAGAG 
AAACAGTGTG AGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
GACCTCAGTG GGAATGAACT AGGAGACTTT CAAAGCCTTG GCTCTCTACT GAAGAACCAA 
CAATTCAAGC TGGAGAAACT AATTCTGTGT GTTTGCAGTA TTAAAGAGAA GCAGTGTGAG 
ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAGTGGG 
AATGAACTCA AGCACAGAAG AGTGAAGCAA TTTTTTGATG TGCTGAAAAA ATCAACCTGT 
AAACTGGAGC GACTGAGGTT AAGATACTGT AGTATACAAG CCGATGATTG TACTGATCTG 
GCTTCAGCTC TGAAATCAAA CCCATCCCAC CTGAGAGAAC TGGACCTCAG TGGAAACAAT 
CTAGGAGACT CTGGAGTGAA AAAACTCAGT GATTTACTGA TAAACCAACA ATTCAAGCTG 
GAGAAACTAC GTCTGAGTTA TTGCAGTATT ACAGAGAAAC AGTGTGTGAT CCTGACTTCA 
GCTCTGAAAT CAAACCCATC ACACCTGAGA GAACTGAACC TCAATGGGAA TAGACTAGGA 
GACTCTGGAG CTGATGGCCT TAACCATCTG CTGAGCAGTG TGGATTGCAA GCTAAATAAA 
CTACATCTGT GTGATTGCAG TATTACAGAG AAACAGTTGC TCATCCCAAT CACAGCTACG 
TGTATGGGCC TATTACACCT GAGAGAACTG GACCTCAGTA AGAACCAAAT AAAAAACCAA 
GGAGTGTACA TGCTGTGTGA TGTGCTGAAA AACCCACGCT GTAGACTCAA GAGGTTAAGA 
CTAAATGACT GTGGCATTAC AGATGTTACA CCCTTAACTC AGTCTTTGAC CAAGGACAAA 
GCATTGAAGT TCTTAAAAGA GCTTGAACTG AGTAAAAATC AAATGGGATT CTTCGAAAAG 
CAAAAACTCA GTGACCTGCT GCGAGACTCA AACTGTAAAC TGAGGCTTGA GGAAGAAGAA 
GGTTATTTTA GAGCTGGTGT AAAACGGGTT ACTACATTTT TGATGCCTCC ATGGTCAAAA 
GTTCAAAAAC CTGTGGATCT CACAGGAGAG GAAAGTTCAT CATCAGATGA GGACGCCTCT 
GAAGATGAGA CTGATCAGGC AGAGGAGGCT GATAAGCTCT CAAATGAGGC GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325104.1
CDS	65..7921
Translation	MSNNNLQDSGVKLLCTALKNVKCELEVLRISYCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGILGKNPLLLKELNLSDCELGDTGVNQLSALLQDKHCKPKTLMLRGCGLKEETCSALATVLGSDTSLKELDMSNNNLQDLGVKKLQSGLENTNCTLEKLSLSDCNISEEGYKALASALRSNPSHLIELNLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAAEEFCQFVTGIVGKNPLLLKELYLSDHELGDTRVNQLSALLQDKHCTVSTLILHNCGLTKESCSALASALSSDTSLKELDLCNNNLQDSGVKKLQSELQNTNWRLKKLRLSKCDLTEESCSALASVLTSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSKLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELIELLQDPLCTLKTLRFLSPAADEACQFVTGIVGKNLLLLKELNLSDRQLGDTRVNQLSALLQDKHCTLNTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLKFSGISITAEGCAALTSAFSSNPSHLIELNLSGNTLEDSGVTEISSLLANSQCMLKILSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_021469429.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469429.1

ORF Insert Sequence:

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACAACGGGGT GATTGCTGTA AATTAAAAAC CGTCAGGTTT 
TTAAAGAGTT CTGCTGCACA GGAAGCGTGT GACTATCTTA CTAAAGCTGT GGGTAAAAGT 
CCATTATTAC TGACAAAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAA GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTGAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAGATCTGT 
GAGGGGCTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ACCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACTTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGAAGCT CACTGACTTA 
CTGCAGGATC CACACTGTAC CCTGAATACA CTGAGGTTTT TGAGTCCTGC CGCAGAAGAA 
GCCTGTCAGT TTGTGACTGG AATTGTGGGT AAAAACCCGA TACTTCTGAA AGAACTGAAT 
CTGAGTGATC ATGAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AAAAACAGCT TTATCTCAGA GGAAGATTGT 
CGTGTTCTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGAGAGAGCT GAATTTGAAT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCAACA CTCTGTTTCA AAACCAACAG 
TGCAGACTGG AAAAGCTGAA GTTTAGCGGC ATCAGTATCA CAGCAGAAGG TTGTGCTGCT 
TTGACTTCAG CATTTAGCTC AAACCCGTCA CACCTGATAG AGCTAAATCT GAGTGGGAAT 
ACACTAGAAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC ACAGTGCATG 
CTGAAGATAC TCAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGCT TTGATTCCAG AGTGTGCTCT GAAAACATTG 
AGGTTTTTGG GTCCTGCAGC AGAAGAAGCC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCGTTAC TCCTGAAAGA ACTGAATCTG AGTGATCGTA AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC CCAACACACT TCAGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAA TTTAAACCCT 
TCACACCTAA TAGAGCTCAA TCTGAGTGGG AATAAACTAA GAGACTCAGG AGTAGAGGAG 
ATCTGTCCTC TGCTGAAAAA TACACAGTGC AGACTGGAGA GACTAAGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTAAA GGAGCTCACT 
GACTTACTGC AGGATGAGCG ATATAGATTG AAGACCCTCA GGCTTTTGAA AACTACTGCA 
GCACAAGAAG CGTGTGACTA TCTTACTAAA GTTGTGGGTA AAAGTCCATT ATTACTGACA 
GAACTGGATC TGAGTGAAGA TAAATTAGGA GATCTGGATG GAGAGAAACT CTCTGCTCTT 
CTGATGGACT CTCATAGTAA AGTGGAGAAA ATCAAGCTGA ATAATTGTAA GCAGATTGAA 
AAAGGCTGTT CAGTACTAGC TGCCGTTCTC TCCTCAAAAA CAATCCTAAA AGAGATGAAC 
CTGAACAACA GTCGTCTGCT GGACTCAGGA GTCTTAGAGA TCTGTGAGGG ACTGAAGAAC 
CCTGTGTGTG AGCTGAAGAT TCTCAAGCTC AGTAAGTGTG ATTTAACAGA GGAAAGCTGT 
TCAGCTCTGG CTTCAGTTCT CAGATCAGAC TCCAGCAGTC TGAAGGATCT TGACCTGAGC 
AATAATAATC TGCAGGATTC AGGAGTGAAG TTGCTCTCTG ATGGACTAAA GCACAGTAAA 
CTGAAGAGAC TCAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAC TTACTGCAGG ATCCACACTG TACACTAAAG 
ACACTGAGTT GTTTTAGTCC TGCTGCTGAT GAAGGCTGTC AGTTTGTTAC TGGAATTGTG 
GGTAAAAACC CGTTACTACT GAAAGAACTG AATCTGAGTG ATCGTGAGCT AGGAGACACA 
CGGGTGAATC AACTCTCTGC TCTACTGCAA GATAAACACT GTACACTCAA TATACTCACG 
CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAATTTA 
AACCCTTCAC ACCTGATAGA GCTCAATCTG AGTGGAAATA AACTAGGAGA CTCAGGAGTA 
GAGAAGATCT GTCCTCTGCT GAAAAATACC CAGTGCAGAT TGGAGAAATT AAATCTTTCA 
GACTGCAGCA TCACTGGAAA AGGTTATGAA ACATTGATTT CAGCTTTGAA GTCAAACTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGGA AATCAGGAGT GCAGAAGCTC 
ACTGATTTGC TACGAGCTGG AAGCTGTAAA TTAAAAACCA TCAGGACTTT GAAAGGTTCC 
GCTGCACAGG AAGCGTGTGA CTATCTAACT AAAGCTGTGG GTAAAAGTCC ATTATTACTG 
ACAGAACTGG ATCTGAGTGA AGATAAATTA GGAGATCTGG ATGGAGAGAA ACTCTCTGCT 
CTTCTGATGG ACTCTCATAG CAAAGTGGAG AAAATCAGGA TGAATAACTG TAAAAAGCTG 
ACAGAGAAAA GCTGTTTAGT TCTAGCTGCT GTTCTTTCAT CCAAAACCAT CTTGAAAGAG 
ATGAACCTAA ACAACAGTCG TCTGCTGGAC TCAGGAGTCA AAGTGATCTG TGAGGGACTG 
AAGAACTCTA AGCTGGAGAT TTTAAAACTT TCAAATTGTA GTATTACAGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC TGGAGTAAAG GAGCTCACTG AGTTACTGCA GAGTTCGTGG 
ATTCCATGGA AAAAAGTGAA GTGTTCAATG AGGTTTTTGA GTCCTGCTGC TGATGAATTT 
TGTCAGTTTG TGTCTGGAAT TGTGGGTAAA AACCCGTTAC TCCTGAAAGA ACTGAATCTG 
AGTGATCATG TACTAGCAGA CACACAAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA 
CACTGCAAAC TCAACACACT CATGCTGAAT AATAACAGGA TTACAGCAGA AGGTTGTGCT 
GCTTTAACTT CAGCATTTAA TTTAAATCCT TCACACCTGA TAGAGCTCAA TCTGAGTGGG 
AATAAACTAG AAGACTCAGG AGTAGAGAAG ATCTGTCCTC TGCTGGAAAA TATACAATGC 
CGATTAGAGA AACTGAGTCT TTCAGACTGC AGTATCAGGG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GATTTACTGC AGAGTAGAAG CTGTAAATTC 
AGGTTTCTGA AGAGTTCTGC TGCACAGGAA GCATGCGAGT ATCTTACTAA TGTTCTGGGT 
ATAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG TTAAATTAGG AGATCTGGAC 
TGGGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGCA AAGTGGAGAA AATCAAGCTA 
AATAATTGTG AGCTGACAGA GAAAAGCTGT TCAGTTCTAG CTGCTGTTCT CTCCTCCAAA 
ACCATCCTGA AAGAGCTGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCAGAAAG 
ATCTGTGAGG GACTGAAGAA TCCTGTGTGT GAGCTGAAGA TACTCAATCT TTCAGACTGC 
AGTATTACAG AAGAAGGTTA TGAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GAGTTACTGC AGGATCCACA CTGTGCACTG AAGACACTGA GATTTTTAAG AAGTCCTGCT 
GCTGATGAAG GCTGTCAGTT AGTAACTGGA GTTGTGGGTA AAAACCCGTT ACTCCTGAAG 
GAACTGAATC TGAGTGATCA TGAGCTAGGA GACACACGAG TGAATCAACT CTCTGCTCTA 
CTGCAGGATA AACACTGCCA GATCAACACA CTCAATCTGA GTTATTGCAG TATTACAGAG 
AAACAGTGTG AGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
GACCTCAGTG GGAATGAACT AGGAGACTTT CAAAGCCTTG GCTCTCTACT GAAGAACCAA 
CAATTCAAGC TGGAGAAACT AATTCTGTGT GTTTGCAGTA TTAAAGAGAA GCAGTGTGAG 
ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAGTGGG 
AATGAACTCA AGCACAGAAG AGTGAAGCAA TTTTTTGATG TGCTGAAAAA ATCAACCTGT 
AAACTGGAGC GACTGAGGTT AAGATACTGT AGTATACAAG CCGATGATTG TACTGATCTG 
GCTTCAGCTC TGAAATCAAA CCCATCCCAC CTGAGAGAAC TGGACCTCAG TGGAAACAAT 
CTAGGAGACT CTGGAGTGAA AAAACTCAGT GATTTACTGA TAAACCAACA ATTCAAGCTG 
GAGAAACTAC GTCTGAGTTA TTGCAGTATT ACAGAGAAAC AGTGTGTGAT CCTGACTTCA 
GCTCTGAAAT CAAACCCATC ACACCTGAGA GAACTGAACC TCAATGGGAA TAGACTAGGA 
GACTCTGGAG CTGATGGCCT TAACCATCTG CTGAGCAGTG TGGATTGCAA GCTAAATAAA 
CTACATCTGT GTGATTGCAG TATTACAGAG AAACAGTTGC TCATCCCAAT CACAGCTACG 
TGTATGGGCC TATTACACCT GAGAGAACTG GACCTCAGTA AGAACCAAAT AAAAAACCAA 
GGAGTGTACA TGCTGTGTGA TGTGCTGAAA AACCCACGCT GTAGACTCAA GAGGTTAAGA 
CTAAATGACT GTGGCATTAC AGATGTTACA CCCTTAACTC AGTCTTTGAC CAAGGACAAA 
GCATTGAAGT TCTTAAAAGA GCTTGAACTG AGTAAAAATC AAATGGGATT CTTCGAAAAG 
CAAAAACTCA GTGACCTGCT GCGAGACTCA AACTGTAAAC TGAGGCTTGA GGAAGAAGAA 
GGTTATTTTA GAGCTGGTGT AAAACGGGTT ACTACATTTT TGATGCCTCC ATGGTCAAAA 
GTTCAAAAAC CTGTGGATCT CACAGGAGAG GAAAGTTCAT CATCAGATGA GGACGCCTCT 
GAAGATGAGA CTGATCAGGC AGAGGAGGCT GATAAGCTCT CAAATGAGGC GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_021469430.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325105.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X11, mRNA.

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CloneID	ODa151253
Clone ID Related Accession (Same CDS sequence)	XM_021469430.1
Accession Version	XM_021469430.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein si:ch211-11p18.6 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TTCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGCTTTG ATTCCAGAGT GTGCTCTGAA AACATTGAGG 
TTTTTGGGTC CTGCAGCAGA AGAAGCCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTAAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTAAACCCA ACACACTTCA GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTAACTTCAG CATTTAATTT AAACCCTTCA 
CACCTAATAG AGCTCAATCT GAGTGGGAAT AAACTAAGAG ACTCAGGAGT AGAGGAGATC 
TGTCCTCTGC TGAAAAATAC ACAGTGCAGA CTGGAGAGAC TAAGTCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTAAAGGA GCTCACTGAC 
TTACTGCAGG ATGAGCGATA TAGATTGAAG ACCCTCAGGC TTTTGAAAAC TACTGCAGCA 
CAAGAAGCGT GTGACTATCT TACTAAAGTT GTGGGTAAAA GTCCATTATT ACTGACAGAA 
CTGGATCTGA GTGAAGATAA ATTAGGAGAT CTGGATGGAG AGAAACTCTC TGCTCTTCTG 
ATGGACTCTC ATAGTAAAGT GGAGAAAATC AAGCTGAATA ATTGTAAGCA GATTGAAAAA 
GGCTGTTCAG TACTAGCTGC CGTTCTCTCC TCAAAAACAA TCCTAAAAGA GATGAACCTG 
AACAACAGTC GTCTGCTGGA CTCAGGAGTC TTAGAGATCT GTGAGGGACT GAAGAACCCT 
GTGTGTGAGC TGAAGATTCT CAAGCTCAGT AAGTGTGATT TAACAGAGGA AAGCTGTTCA 
GCTCTGGCTT CAGTTCTCAG ATCAGACTCC AGCAGTCTGA AGGATCTTGA CCTGAGCAAT 
AATAATCTGC AGGATTCAGG AGTGAAGTTG CTCTCTGATG GACTAAAGCA CAGTAAACTG 
AAGAGACTCA GTCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGACTTA CTGCAGGATC CACACTGTAC ACTAAAGACA 
CTGAGTTGTT TTAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTTACTGG AATTGTGGGT 
AAAAACCCGT TACTACTGAA AGAACTGAAT CTGAGTGATC GTGAGCTAGG AGACACACGG 
GTGAATCAAC TCTCTGCTCT ACTGCAAGAT AAACACTGTA CACTCAATAT ACTCACGCTG 
AATAATAACA GGATTACAGC AGAAGGTTGT GCTGCTTTGA CTTCAGCATT TAATTTAAAC 
CCTTCACACC TGATAGAGCT CAATCTGAGT GGAAATAAAC TAGGAGACTC AGGAGTAGAG 
AAGATCTGTC CTCTGCTGAA AAATACCCAG TGCAGATTGG AGAAATTAAA TCTTTCAGAC 
TGCAGCATCA CTGGAAAAGG TTATGAAACA TTGATTTCAG CTTTGAAGTC AAACTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGGAAAT CAGGAGTGCA GAAGCTCACT 
GATTTGCTAC GAGCTGGAAG CTGTAAATTA AAAACCATCA GGACTTTGAA AGGTTCCGCT 
GCACAGGAAG CGTGTGACTA TCTAACTAAA GCTGTGGGTA AAAGTCCATT ATTACTGACA 
GAACTGGATC TGAGTGAAGA TAAATTAGGA GATCTGGATG GAGAGAAACT CTCTGCTCTT 
CTGATGGACT CTCATAGCAA AGTGGAGAAA ATCAGGATGA ATAACTGTAA AAAGCTGACA 
GAGAAAAGCT GTTTAGTTCT AGCTGCTGTT CTTTCATCCA AAACCATCTT GAAAGAGATG 
AACCTAAACA ACAGTCGTCT GCTGGACTCA GGAGTCAAAG TGATCTGTGA GGGACTGAAG 
AACTCTAAGC TGGAGATTTT AAAACTTTCA AATTGTAGTA TTACAGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCTGG AGTAAAGGAG CTCACTGAGT TACTGCAGAG TTCGTGGATT 
CCATGGAAAA AAGTGAAGTG TTCAATGAGG TTTTTGAGTC CTGCTGCTGA TGAATTTTGT 
CAGTTTGTGT CTGGAATTGT GGGTAAAAAC CCGTTACTCC TGAAAGAACT GAATCTGAGT 
GATCATGTAC TAGCAGACAC ACAAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGCAAACTCA ACACACTCAT TCTTTCAGAC TGCAGTATCA GTGGAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAAGAGCTC ACTGAGTTAC TGCAGGATCC ACACTGTACA 
CTGAAGACAC TGAGGTTTTT GAGTGATGAT GCAGATGAAG CCTGTGAACA TTTGTATAAA 
GTTCTGGGTG GAAACCTGTT ACTCCTGAGA GAGCTGAATC TGACTAAACA TGATCTTGGA 
CCATCAGGAT TAGAGAAACT TGCTGCAGTA CTGAAAGATA AACACTGTAA ACTCAACACA 
CTGATTCTGA ATAACAGCAC TATCTCAGAG GAAGATTTTC ATGTTCTGAC TGAAGCTTTT 
AATTCAAACC CAACAAATCT GACAGAGCTG AACTTGAGTG GGACTAAACT CACAAACTCA 
GGAATGAAGA GCTTCACAAC TCTGTTTCAA AACCAACAGT GTAGATTGGA AAAGCTGAAG 
TTCAGCAGCA TGTGTATTAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAACTCA 
AACCCTTCAC ACCTGAAAGA GCTCAATCTG AGTGGGAATA AACTAGGAGA CTCAGGAGTA 
AGAAAAATCT CCAGTCTACT GAGAAACTCG CAGTGCATGC TGAAGATACT CGGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TTACATCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACCATCAGG AGTGAAGGAG 
CTCACTGGCT TACTACTCGA TCCACACTGT ACACTGACGA CACTGAGGTT TTTGAGTCCT 
GCTGCTGATG AATTCTGTCA GTTTGTGTCT GGAATTGTGG GTAAATACCC ATTACTCCTG 
AAAGAACTGA ATCTGAGTGA TCGTGGACTA GGGGACACAC GAGTGAATCA ACTCTCTGCT 
CTACTGCAGG ATAAACACTT TAGACTCAAC ACACTCATGC TGAATAATAA CAGGATTACA 
GCAGAAGGTT GTGCTGCTTT AACTTCAGCA TTTAATTTAA ATCCTTCACA CCTGATAGAG 
CTCAATCTGA GTGGGAATAA ACTAGAAGAC TCAGGAGTAG AGAAGATCTG TCCTCTGCTG 
GAAAATATAC AATGCCGATT AGAGAAACTG AGTCTTTCAG ACTGCAGTAT CAGGGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAGC TCACTGATTT ACTGCAGAGT 
AGAAGCTGTA AATTCAGGTT TCTGAAGAGT TCTGCTGCAC AGGAAGCATG CGAGTATCTT 
ACTAATGTTC TGGGTATAAG TCCATTATTA CTGACAGAAC TGGATCTGAG TGAAGTTAAA 
TTAGGAGATC TGGACTGGGA GAAACTCTCT GCTCTTCTGA TGGACTCTCA TAGCAAAGTG 
GAGAAAATCA AGCTAAATAA TTGTGAGCTG ACAGAGAAAA GCTGTTCAGT TCTAGCTGCT 
GTTCTCTCCT CCAAAACCAT CCTGAAAGAG CTGAACCTGA ACAACAGTCG TCTGCTGGAC 
TCAGGAGTCA GAAAGATCTG TGAGGGACTG AAGAATCCTG TGTGTGAGCT GAAGATACTC 
AATCTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATGAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTG CACTGAAGAC ACTGAGATTT 
TTAAGAAGTC CTGCTGCTGA TGAAGGCTGT CAGTTAGTAA CTGGAGTTGT GGGTAAAAAC 
CCGTTACTCC TGAAGGAACT GAATCTGAGT GATCATGAGC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGCCAGATCA ACACACTCAA TCTGAGTTAT 
TGCAGTATTA CAGAGAAACA GTGTGAGATC CTGACTTCAG CTCTGAAATC AAACCCATCA 
CACCTGAGAG AACTGGACCT CAGTGGGAAT GAACTAGGAG ACTTTCAAAG CCTTGGCTCT 
CTACTGAAGA ACCAACAATT CAAGCTGGAG AAACTAATTC TGTGTGTTTG CAGTATTAAA 
GAGAAGCAGT GTGAGATCCT GACTTCAGCT CTGAAATCAA ACCCATCACA CCTGAGAGAA 
CTGAACCTCA GTGGGAATGA ACTCAAGCAC AGAAGAGTGA AGCAATTTTT TGATGTGCTG 
AAAAAATCAA CCTGTAAACT GGAGCGACTG AGGTTAAGAT ACTGTAGTAT ACAAGCCGAT 
GATTGTACTG ATCTGGCTTC AGCTCTGAAA TCAAACCCAT CCCACCTGAG AGAACTGGAC 
CTCAGTGGAA ACAATCTAGG AGACTCTGGA GTGAAAAAAC TCAGTGATTT ACTGATAAAC 
CAACAATTCA AGCTGGAGAA ACTACGTCTG AGTTATTGCA GTATTACAGA GAAACAGTGT 
GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAAT 
GGGAATAGAC TAGGAGACTC TGGAGCTGAT GGCCTTAACC ATCTGCTGAG CAGTGTGGAT 
TGCAAGCTAA ATAAACTACA TCTGTGTGAT TGCAGTATTA CAGAGAAACA GTTGCTCATC 
CCAATCACAG CTACGTGTAT GGGCCTATTA CACCTGAGAG AACTGGACCT CAGTAAGAAC 
CAAATAAAAA ACCAAGGAGT GTACATGCTG TGTGATGTGC TGAAAAACCC ACGCTGTAGA 
CTCAAGAGGT TAAGACTAAA TGACTGTGGC ATTACAGATG TTACACCCTT AACTCAGTCT 
TTGACCAAGG ACAAAGCATT GAAGTTCTTA AAAGAGCTTG AACTGAGTAA AAATCAAATG 
GGATTCTTCG AAAAGCAAAA ACTCAGTGAC CTGCTGCGAG ACTCAAACTG TAAACTGAGG 
CTTGAGGAAG AAGAAGGTTA TTTTAGAGCT GGTGTAAAAC GGGTTACTAC ATTTTTGATG 
CCTCCATGGT CAAAAGTTCA AAAACCTGTG GATCTCACAG GAGAGGAAAG TTCATCATCA 
GATGAGGACG CCTCTGAAGA TGAGACTGAT CAGGCAGAGG AGGCTGATAA GCTCTCAAAT 
GAGGCGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325105.1
CDS	65..7576
Translation	MSNNNLQDSGVKLLCTALKNVKCELEVLRISYCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGILGKNPLLLKELNLSDCELGDTGVNQLSALLQDKHCKPKTLMLRGCGLKEETCSALATVLGSDTSLKELDMSNNNLQDLGVKKLQSGLENTNCTLEKLSLSDCNISEEGYKALASALRSNPSHLIELNLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAAEEFCQFVTGIVGKNPLLLKELYLSDHELGDTRVNQLSALLQDKHCTVSTLILHNCGLTKESCSALASALSSDTSLKELDLCNNNLQDSGVKKLQSELQNTNWRLKKLRLSKCDLTEESCSALASVLTSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSKLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELIELLQDPLCTLKTLRFLSPAADEACQFVTGIVGKNLLLLKELNLSDRQLGDTRVNQLSALLQDKHCTLNTLILSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACEHLYKVLGGNLLLLRELNLTKHDLGPSGLEKLAAVLKDKHCKLNTLILNNSTISEEDFHVLTEAFNSNPTNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_021469430.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469430.1

ORF Insert Sequence:

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TTCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGCTTTG ATTCCAGAGT GTGCTCTGAA AACATTGAGG 
TTTTTGGGTC CTGCAGCAGA AGAAGCCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTAAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTAAACCCA ACACACTTCA GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTAACTTCAG CATTTAATTT AAACCCTTCA 
CACCTAATAG AGCTCAATCT GAGTGGGAAT AAACTAAGAG ACTCAGGAGT AGAGGAGATC 
TGTCCTCTGC TGAAAAATAC ACAGTGCAGA CTGGAGAGAC TAAGTCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTAAAGGA GCTCACTGAC 
TTACTGCAGG ATGAGCGATA TAGATTGAAG ACCCTCAGGC TTTTGAAAAC TACTGCAGCA 
CAAGAAGCGT GTGACTATCT TACTAAAGTT GTGGGTAAAA GTCCATTATT ACTGACAGAA 
CTGGATCTGA GTGAAGATAA ATTAGGAGAT CTGGATGGAG AGAAACTCTC TGCTCTTCTG 
ATGGACTCTC ATAGTAAAGT GGAGAAAATC AAGCTGAATA ATTGTAAGCA GATTGAAAAA 
GGCTGTTCAG TACTAGCTGC CGTTCTCTCC TCAAAAACAA TCCTAAAAGA GATGAACCTG 
AACAACAGTC GTCTGCTGGA CTCAGGAGTC TTAGAGATCT GTGAGGGACT GAAGAACCCT 
GTGTGTGAGC TGAAGATTCT CAAGCTCAGT AAGTGTGATT TAACAGAGGA AAGCTGTTCA 
GCTCTGGCTT CAGTTCTCAG ATCAGACTCC AGCAGTCTGA AGGATCTTGA CCTGAGCAAT 
AATAATCTGC AGGATTCAGG AGTGAAGTTG CTCTCTGATG GACTAAAGCA CAGTAAACTG 
AAGAGACTCA GTCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGACTTA CTGCAGGATC CACACTGTAC ACTAAAGACA 
CTGAGTTGTT TTAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTTACTGG AATTGTGGGT 
AAAAACCCGT TACTACTGAA AGAACTGAAT CTGAGTGATC GTGAGCTAGG AGACACACGG 
GTGAATCAAC TCTCTGCTCT ACTGCAAGAT AAACACTGTA CACTCAATAT ACTCACGCTG 
AATAATAACA GGATTACAGC AGAAGGTTGT GCTGCTTTGA CTTCAGCATT TAATTTAAAC 
CCTTCACACC TGATAGAGCT CAATCTGAGT GGAAATAAAC TAGGAGACTC AGGAGTAGAG 
AAGATCTGTC CTCTGCTGAA AAATACCCAG TGCAGATTGG AGAAATTAAA TCTTTCAGAC 
TGCAGCATCA CTGGAAAAGG TTATGAAACA TTGATTTCAG CTTTGAAGTC AAACTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGGAAAT CAGGAGTGCA GAAGCTCACT 
GATTTGCTAC GAGCTGGAAG CTGTAAATTA AAAACCATCA GGACTTTGAA AGGTTCCGCT 
GCACAGGAAG CGTGTGACTA TCTAACTAAA GCTGTGGGTA AAAGTCCATT ATTACTGACA 
GAACTGGATC TGAGTGAAGA TAAATTAGGA GATCTGGATG GAGAGAAACT CTCTGCTCTT 
CTGATGGACT CTCATAGCAA AGTGGAGAAA ATCAGGATGA ATAACTGTAA AAAGCTGACA 
GAGAAAAGCT GTTTAGTTCT AGCTGCTGTT CTTTCATCCA AAACCATCTT GAAAGAGATG 
AACCTAAACA ACAGTCGTCT GCTGGACTCA GGAGTCAAAG TGATCTGTGA GGGACTGAAG 
AACTCTAAGC TGGAGATTTT AAAACTTTCA AATTGTAGTA TTACAGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCTGG AGTAAAGGAG CTCACTGAGT TACTGCAGAG TTCGTGGATT 
CCATGGAAAA AAGTGAAGTG TTCAATGAGG TTTTTGAGTC CTGCTGCTGA TGAATTTTGT 
CAGTTTGTGT CTGGAATTGT GGGTAAAAAC CCGTTACTCC TGAAAGAACT GAATCTGAGT 
GATCATGTAC TAGCAGACAC ACAAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGCAAACTCA ACACACTCAT TCTTTCAGAC TGCAGTATCA GTGGAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAAGAGCTC ACTGAGTTAC TGCAGGATCC ACACTGTACA 
CTGAAGACAC TGAGGTTTTT GAGTGATGAT GCAGATGAAG CCTGTGAACA TTTGTATAAA 
GTTCTGGGTG GAAACCTGTT ACTCCTGAGA GAGCTGAATC TGACTAAACA TGATCTTGGA 
CCATCAGGAT TAGAGAAACT TGCTGCAGTA CTGAAAGATA AACACTGTAA ACTCAACACA 
CTGATTCTGA ATAACAGCAC TATCTCAGAG GAAGATTTTC ATGTTCTGAC TGAAGCTTTT 
AATTCAAACC CAACAAATCT GACAGAGCTG AACTTGAGTG GGACTAAACT CACAAACTCA 
GGAATGAAGA GCTTCACAAC TCTGTTTCAA AACCAACAGT GTAGATTGGA AAAGCTGAAG 
TTCAGCAGCA TGTGTATTAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAACTCA 
AACCCTTCAC ACCTGAAAGA GCTCAATCTG AGTGGGAATA AACTAGGAGA CTCAGGAGTA 
AGAAAAATCT CCAGTCTACT GAGAAACTCG CAGTGCATGC TGAAGATACT CGGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TTACATCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACCATCAGG AGTGAAGGAG 
CTCACTGGCT TACTACTCGA TCCACACTGT ACACTGACGA CACTGAGGTT TTTGAGTCCT 
GCTGCTGATG AATTCTGTCA GTTTGTGTCT GGAATTGTGG GTAAATACCC ATTACTCCTG 
AAAGAACTGA ATCTGAGTGA TCGTGGACTA GGGGACACAC GAGTGAATCA ACTCTCTGCT 
CTACTGCAGG ATAAACACTT TAGACTCAAC ACACTCATGC TGAATAATAA CAGGATTACA 
GCAGAAGGTT GTGCTGCTTT AACTTCAGCA TTTAATTTAA ATCCTTCACA CCTGATAGAG 
CTCAATCTGA GTGGGAATAA ACTAGAAGAC TCAGGAGTAG AGAAGATCTG TCCTCTGCTG 
GAAAATATAC AATGCCGATT AGAGAAACTG AGTCTTTCAG ACTGCAGTAT CAGGGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAGC TCACTGATTT ACTGCAGAGT 
AGAAGCTGTA AATTCAGGTT TCTGAAGAGT TCTGCTGCAC AGGAAGCATG CGAGTATCTT 
ACTAATGTTC TGGGTATAAG TCCATTATTA CTGACAGAAC TGGATCTGAG TGAAGTTAAA 
TTAGGAGATC TGGACTGGGA GAAACTCTCT GCTCTTCTGA TGGACTCTCA TAGCAAAGTG 
GAGAAAATCA AGCTAAATAA TTGTGAGCTG ACAGAGAAAA GCTGTTCAGT TCTAGCTGCT 
GTTCTCTCCT CCAAAACCAT CCTGAAAGAG CTGAACCTGA ACAACAGTCG TCTGCTGGAC 
TCAGGAGTCA GAAAGATCTG TGAGGGACTG AAGAATCCTG TGTGTGAGCT GAAGATACTC 
AATCTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATGAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTG CACTGAAGAC ACTGAGATTT 
TTAAGAAGTC CTGCTGCTGA TGAAGGCTGT CAGTTAGTAA CTGGAGTTGT GGGTAAAAAC 
CCGTTACTCC TGAAGGAACT GAATCTGAGT GATCATGAGC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGCCAGATCA ACACACTCAA TCTGAGTTAT 
TGCAGTATTA CAGAGAAACA GTGTGAGATC CTGACTTCAG CTCTGAAATC AAACCCATCA 
CACCTGAGAG AACTGGACCT CAGTGGGAAT GAACTAGGAG ACTTTCAAAG CCTTGGCTCT 
CTACTGAAGA ACCAACAATT CAAGCTGGAG AAACTAATTC TGTGTGTTTG CAGTATTAAA 
GAGAAGCAGT GTGAGATCCT GACTTCAGCT CTGAAATCAA ACCCATCACA CCTGAGAGAA 
CTGAACCTCA GTGGGAATGA ACTCAAGCAC AGAAGAGTGA AGCAATTTTT TGATGTGCTG 
AAAAAATCAA CCTGTAAACT GGAGCGACTG AGGTTAAGAT ACTGTAGTAT ACAAGCCGAT 
GATTGTACTG ATCTGGCTTC AGCTCTGAAA TCAAACCCAT CCCACCTGAG AGAACTGGAC 
CTCAGTGGAA ACAATCTAGG AGACTCTGGA GTGAAAAAAC TCAGTGATTT ACTGATAAAC 
CAACAATTCA AGCTGGAGAA ACTACGTCTG AGTTATTGCA GTATTACAGA GAAACAGTGT 
GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAAT 
GGGAATAGAC TAGGAGACTC TGGAGCTGAT GGCCTTAACC ATCTGCTGAG CAGTGTGGAT 
TGCAAGCTAA ATAAACTACA TCTGTGTGAT TGCAGTATTA CAGAGAAACA GTTGCTCATC 
CCAATCACAG CTACGTGTAT GGGCCTATTA CACCTGAGAG AACTGGACCT CAGTAAGAAC 
CAAATAAAAA ACCAAGGAGT GTACATGCTG TGTGATGTGC TGAAAAACCC ACGCTGTAGA 
CTCAAGAGGT TAAGACTAAA TGACTGTGGC ATTACAGATG TTACACCCTT AACTCAGTCT 
TTGACCAAGG ACAAAGCATT GAAGTTCTTA AAAGAGCTTG AACTGAGTAA AAATCAAATG 
GGATTCTTCG AAAAGCAAAA ACTCAGTGAC CTGCTGCGAG ACTCAAACTG TAAACTGAGG 
CTTGAGGAAG AAGAAGGTTA TTTTAGAGCT GGTGTAAAAC GGGTTACTAC ATTTTTGATG 
CCTCCATGGT CAAAAGTTCA AAAACCTGTG GATCTCACAG GAGAGGAAAG TTCATCATCA 
GATGAGGACG CCTCTGAAGA TGAGACTGAT CAGGCAGAGG AGGCTGATAA GCTCTCAAAT 
GAGGCGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_021469431.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325106.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X12, mRNA.

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CloneID	ODa151254
Clone ID Related Accession (Same CDS sequence)	XM_021469431.1
Accession Version	XM_021469431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein si:ch211-11p18.6 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG CTTTGATTCC AGAGTGTGCT 
CTGAAAACAT TGAGGTTTTT GGGTCCTGCA GCAGAAGAAG CCTGTCAGTT TGTGACTGGA 
ATTGTGGGTA AAAACCCGTT ACTCCTGAAA GAACTGAATC TGAGTGATCG TAAACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGTAA ACCCAACACA 
CTTCAGCTGA ATAATAACAG GATTACAGCA GAAGGTTGTG CTGCTTTAAC TTCAGCATTT 
AATTTAAACC CTTCACACCT AATAGAGCTC AATCTGAGTG GGAATAAACT AAGAGACTCA 
GGAGTAGAGG AGATCTGTCC TCTGCTGAAA AATACACAGT GCAGACTGGA GAGACTAAGT 
CTTTCAGACT GCAGTATCAG TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACA ATCAGGAGTA 
AAGGAGCTCA CTGACTTACT GCAGGATGAG CGATATAGAT TGAAGACCCT CAGGCTTTTG 
AAAACTACTG CAGCACAAGA AGCGTGTGAC TATCTTACTA AAGTTGTGGG TAAAAGTCCA 
TTATTACTGA CAGAACTGGA TCTGAGTGAA GATAAATTAG GAGATCTGGA TGGAGAGAAA 
CTCTCTGCTC TTCTGATGGA CTCTCATAGT AAAGTGGAGA AAATCAAGCT GAATAATTGT 
AAGCAGATTG AAAAAGGCTG TTCAGTACTA GCTGCCGTTC TCTCCTCAAA AACAATCCTA 
AAAGAGATGA ACCTGAACAA CAGTCGTCTG CTGGACTCAG GAGTCTTAGA GATCTGTGAG 
GGACTGAAGA ACCCTGTGTG TGAGCTGAAG ATTCTCAAGC TCAGTAAGTG TGATTTAACA 
GAGGAAAGCT GTTCAGCTCT GGCTTCAGTT CTCAGATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGTTGCTCTC TGATGGACTA 
AAGCACAGTA AACTGAAGAG ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG ACTTACTGCA GGATCCACAC 
TGTACACTAA AGACACTGAG TTGTTTTAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTT 
ACTGGAATTG TGGGTAAAAA CCCGTTACTA CTGAAAGAAC TGAATCTGAG TGATCGTGAG 
CTAGGAGACA CACGGGTGAA TCAACTCTCT GCTCTACTGC AAGATAAACA CTGTACACTC 
AATATACTCA CGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAACCCTTC ACACCTGATA GAGCTCAATC TGAGTGGAAA TAAACTAGGA 
GACTCAGGAG TAGAGAAGAT CTGTCCTCTG CTGAAAAATA CCCAGTGCAG ATTGGAGAAA 
TTAAATCTTT CAGACTGCAG CATCACTGGA AAAGGTTATG AAACATTGAT TTCAGCTTTG 
AAGTCAAACT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG GAAATCAGGA 
GTGCAGAAGC TCACTGATTT GCTACGAGCT GGAAGCTGTA AATTAAAAAC CATCAGGACT 
TTGAAAGGTT CCGCTGCACA GGAAGCGTGT GACTATCTAA CTAAAGCTGT GGGTAAAAGT 
CCATTATTAC TGACAGAACT GGATCTGAGT GAAGATAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAT GGACTCTCAT AGCAAAGTGG AGAAAATCAG GATGAATAAC 
TGTAAAAAGC TGACAGAGAA AAGCTGTTTA GTTCTAGCTG CTGTTCTTTC ATCCAAAACC 
ATCTTGAAAG AGATGAACCT AAACAACAGT CGTCTGCTGG ACTCAGGAGT CAAAGTGATC 
TGTGAGGGAC TGAAGAACTC TAAGCTGGAG ATTTTAAAAC TTTCAAATTG TAGTATTACA 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCTGGAGTAA AGGAGCTCAC TGAGTTACTG 
CAGAGTTCGT GGATTCCATG GAAAAAAGTG AAGTGTTCAA TGAGGTTTTT GAGTCCTGCT 
GCTGATGAAT TTTGTCAGTT TGTGTCTGGA ATTGTGGGTA AAAACCCGTT ACTCCTGAAA 
GAACTGAATC TGAGTGATCA TGTACTAGCA GACACACAAG TGAATCAACT CTCTGCTCTA 
CTGCAGGATA AACACTGCAA ACTCAACACA CTCATTCTTT CAGACTGCAG TATCAGTGGA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAAG AGCTCACTGA GTTACTGCAG 
GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTG ATGATGCAGA TGAAGCCTGT 
GAACATTTGT ATAAAGTTCT GGGTGGAAAC CTGTTACTCC TGAGAGAGCT GAATCTGACT 
AAACATGATC TTGGACCATC AGGATTAGAG AAACTTGCTG CAGTACTGAA AGATAAACAC 
TGTAAACTCA ACACACTGAT TCTGAATAAC AGCACTATCT CAGAGGAAGA TTTTCATGTT 
CTGACTGAAG CTTTTAATTC AAACCCAACA AATCTGACAG AGCTGAACTT GAGTGGGACT 
AAACTCACAA ACTCAGGAAT GAAGAGCTTC ACAACTCTGT TTCAAAACCA ACAGTGTAGA 
TTGGAAAAGC TGAAGTTCAG CAGCATGTGT ATTACAGCAG AAGGTTGTGC TGCTTTGACT 
TCAGCATTTA ACTCAAACCC TTCACACCTG AAAGAGCTCA ATCTGAGTGG GAATAAACTA 
GGAGACTCAG GAGTAAGAAA AATCTCCAGT CTACTGAGAA ACTCGCAGTG CATGCTGAAG 
ATACTCGGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTTACA TCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGGCTTACTA CTCGATCCAC ACTGTACACT GACGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAATTC TGTCAGTTTG TGTCTGGAAT TGTGGGTAAA 
TACCCATTAC TCCTGAAAGA ACTGAATCTG AGTGATCGTG GACTAGGGGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTTTAGAC TCAACACACT CATGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAA TTTAAATCCT 
TCACACCTGA TAGAGCTCAA TCTGAGTGGG AATAAACTAG AAGACTCAGG AGTAGAGAAG 
ATCTGTCCTC TGCTGGAAAA TATACAATGC CGATTAGAGA AACTGAGTCT TTCAGACTGC 
AGTATCAGGG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GATTTACTGC AGAGTAGAAG CTGTAAATTC AGGTTTCTGA AGAGTTCTGC TGCACAGGAA 
GCATGCGAGT ATCTTACTAA TGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT 
CTGAGTGAAG TTAAATTAGG AGATCTGGAC TGGGAGAAAC TCTCTGCTCT TCTGATGGAC 
TCTCATAGCA AAGTGGAGAA AATCAAGCTA AATAATTGTG AGCTGACAGA GAAAAGCTGT 
TCAGTTCTAG CTGCTGTTCT CTCCTCCAAA ACCATCCTGA AAGAGCTGAA CCTGAACAAC 
AGTCGTCTGC TGGACTCAGG AGTCAGAAAG ATCTGTGAGG GACTGAAGAA TCCTGTGTGT 
GAGCTGAAGA TACTCAATCT TTCAGACTGC AGTATTACAG AAGAAGGTTA TGAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GAGTTACTGC AGGATCCACA CTGTGCACTG 
AAGACACTGA GATTTTTAAG AAGTCCTGCT GCTGATGAAG GCTGTCAGTT AGTAACTGGA 
GTTGTGGGTA AAAACCCGTT ACTCCTGAAG GAACTGAATC TGAGTGATCA TGAGCTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGCCA GATCAACACA 
CTCAATCTGA GTTATTGCAG TATTACAGAG AAACAGTGTG AGATCCTGAC TTCAGCTCTG 
AAATCAAACC CATCACACCT GAGAGAACTG GACCTCAGTG GGAATGAACT AGGAGACTTT 
CAAAGCCTTG GCTCTCTACT GAAGAACCAA CAATTCAAGC TGGAGAAACT AATTCTGTGT 
GTTTGCAGTA TTAAAGAGAA GCAGTGTGAG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGAA CCTCAGTGGG AATGAACTCA AGCACAGAAG AGTGAAGCAA 
TTTTTTGATG TGCTGAAAAA ATCAACCTGT AAACTGGAGC GACTGAGGTT AAGATACTGT 
AGTATACAAG CCGATGATTG TACTGATCTG GCTTCAGCTC TGAAATCAAA CCCATCCCAC 
CTGAGAGAAC TGGACCTCAG TGGAAACAAT CTAGGAGACT CTGGAGTGAA AAAACTCAGT 
GATTTACTGA TAAACCAACA ATTCAAGCTG GAGAAACTAC GTCTGAGTTA TTGCAGTATT 
ACAGAGAAAC AGTGTGTGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA 
GAACTGAACC TCAATGGGAA TAGACTAGGA GACTCTGGAG CTGATGGCCT TAACCATCTG 
CTGAGCAGTG TGGATTGCAA GCTAAATAAA CTACATCTGT GTGATTGCAG TATTACAGAG 
AAACAGTTGC TCATCCCAAT CACAGCTACG TGTATGGGCC TATTACACCT GAGAGAACTG 
GACCTCAGTA AGAACCAAAT AAAAAACCAA GGAGTGTACA TGCTGTGTGA TGTGCTGAAA 
AACCCACGCT GTAGACTCAA GAGGTTAAGA CTAAATGACT GTGGCATTAC AGATGTTACA 
CCCTTAACTC AGTCTTTGAC CAAGGACAAA GCATTGAAGT TCTTAAAAGA GCTTGAACTG 
AGTAAAAATC AAATGGGATT CTTCGAAAAG CAAAAACTCA GTGACCTGCT GCGAGACTCA 
AACTGTAAAC TGAGGCTTGA GGAAGAAGAA GGTTATTTTA GAGCTGGTGT AAAACGGGTT 
ACTACATTTT TGATGCCTCC ATGGTCAAAA GTTCAAAAAC CTGTGGATCT CACAGGAGAG 
GAAAGTTCAT CATCAGATGA GGACGCCTCT GAAGATGAGA CTGATCAGGC AGAGGAGGCT 
GATAAGCTCT CAAATGAGGC GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325106.1
CDS	65..7231
Translation	MSNNNLQDSGVKLLCTALKNVKCELEVLRISYCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGILGKNPLLLKELNLSDCELGDTGVNQLSALLQDKHCKPKTLMLRGCGLKEETCSALATVLGSDTSLKELDMSNNNLQDLGVKKLQSGLENTNCTLEKLSLSDCNISEEGYKALASALRSNPSHLIELNLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAAEEFCQFVTGIVGKNPLLLKELYLSDHELGDTRVNQLSALLQDKHCTVSTLILHNCGLTKESCSALASALSSDTSLKELDLCNNNLQDSGVKKLQSELQNTNWRLKKLRLSKCDLTEESCSALASVLTSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSKLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACEHLYKVLGGNLLLLRELNLTKHDLGPSGLEKLAAVLKDKHCKLNTLILNNSTISEEDFHVLTEAFNSNPTNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_021469431.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469431.1

ORF Insert Sequence:

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG CTTTGATTCC AGAGTGTGCT 
CTGAAAACAT TGAGGTTTTT GGGTCCTGCA GCAGAAGAAG CCTGTCAGTT TGTGACTGGA 
ATTGTGGGTA AAAACCCGTT ACTCCTGAAA GAACTGAATC TGAGTGATCG TAAACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGTAA ACCCAACACA 
CTTCAGCTGA ATAATAACAG GATTACAGCA GAAGGTTGTG CTGCTTTAAC TTCAGCATTT 
AATTTAAACC CTTCACACCT AATAGAGCTC AATCTGAGTG GGAATAAACT AAGAGACTCA 
GGAGTAGAGG AGATCTGTCC TCTGCTGAAA AATACACAGT GCAGACTGGA GAGACTAAGT 
CTTTCAGACT GCAGTATCAG TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACA ATCAGGAGTA 
AAGGAGCTCA CTGACTTACT GCAGGATGAG CGATATAGAT TGAAGACCCT CAGGCTTTTG 
AAAACTACTG CAGCACAAGA AGCGTGTGAC TATCTTACTA AAGTTGTGGG TAAAAGTCCA 
TTATTACTGA CAGAACTGGA TCTGAGTGAA GATAAATTAG GAGATCTGGA TGGAGAGAAA 
CTCTCTGCTC TTCTGATGGA CTCTCATAGT AAAGTGGAGA AAATCAAGCT GAATAATTGT 
AAGCAGATTG AAAAAGGCTG TTCAGTACTA GCTGCCGTTC TCTCCTCAAA AACAATCCTA 
AAAGAGATGA ACCTGAACAA CAGTCGTCTG CTGGACTCAG GAGTCTTAGA GATCTGTGAG 
GGACTGAAGA ACCCTGTGTG TGAGCTGAAG ATTCTCAAGC TCAGTAAGTG TGATTTAACA 
GAGGAAAGCT GTTCAGCTCT GGCTTCAGTT CTCAGATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGTTGCTCTC TGATGGACTA 
AAGCACAGTA AACTGAAGAG ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG ACTTACTGCA GGATCCACAC 
TGTACACTAA AGACACTGAG TTGTTTTAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTT 
ACTGGAATTG TGGGTAAAAA CCCGTTACTA CTGAAAGAAC TGAATCTGAG TGATCGTGAG 
CTAGGAGACA CACGGGTGAA TCAACTCTCT GCTCTACTGC AAGATAAACA CTGTACACTC 
AATATACTCA CGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAACCCTTC ACACCTGATA GAGCTCAATC TGAGTGGAAA TAAACTAGGA 
GACTCAGGAG TAGAGAAGAT CTGTCCTCTG CTGAAAAATA CCCAGTGCAG ATTGGAGAAA 
TTAAATCTTT CAGACTGCAG CATCACTGGA AAAGGTTATG AAACATTGAT TTCAGCTTTG 
AAGTCAAACT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG GAAATCAGGA 
GTGCAGAAGC TCACTGATTT GCTACGAGCT GGAAGCTGTA AATTAAAAAC CATCAGGACT 
TTGAAAGGTT CCGCTGCACA GGAAGCGTGT GACTATCTAA CTAAAGCTGT GGGTAAAAGT 
CCATTATTAC TGACAGAACT GGATCTGAGT GAAGATAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAT GGACTCTCAT AGCAAAGTGG AGAAAATCAG GATGAATAAC 
TGTAAAAAGC TGACAGAGAA AAGCTGTTTA GTTCTAGCTG CTGTTCTTTC ATCCAAAACC 
ATCTTGAAAG AGATGAACCT AAACAACAGT CGTCTGCTGG ACTCAGGAGT CAAAGTGATC 
TGTGAGGGAC TGAAGAACTC TAAGCTGGAG ATTTTAAAAC TTTCAAATTG TAGTATTACA 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCTGGAGTAA AGGAGCTCAC TGAGTTACTG 
CAGAGTTCGT GGATTCCATG GAAAAAAGTG AAGTGTTCAA TGAGGTTTTT GAGTCCTGCT 
GCTGATGAAT TTTGTCAGTT TGTGTCTGGA ATTGTGGGTA AAAACCCGTT ACTCCTGAAA 
GAACTGAATC TGAGTGATCA TGTACTAGCA GACACACAAG TGAATCAACT CTCTGCTCTA 
CTGCAGGATA AACACTGCAA ACTCAACACA CTCATTCTTT CAGACTGCAG TATCAGTGGA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAAG AGCTCACTGA GTTACTGCAG 
GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTG ATGATGCAGA TGAAGCCTGT 
GAACATTTGT ATAAAGTTCT GGGTGGAAAC CTGTTACTCC TGAGAGAGCT GAATCTGACT 
AAACATGATC TTGGACCATC AGGATTAGAG AAACTTGCTG CAGTACTGAA AGATAAACAC 
TGTAAACTCA ACACACTGAT TCTGAATAAC AGCACTATCT CAGAGGAAGA TTTTCATGTT 
CTGACTGAAG CTTTTAATTC AAACCCAACA AATCTGACAG AGCTGAACTT GAGTGGGACT 
AAACTCACAA ACTCAGGAAT GAAGAGCTTC ACAACTCTGT TTCAAAACCA ACAGTGTAGA 
TTGGAAAAGC TGAAGTTCAG CAGCATGTGT ATTACAGCAG AAGGTTGTGC TGCTTTGACT 
TCAGCATTTA ACTCAAACCC TTCACACCTG AAAGAGCTCA ATCTGAGTGG GAATAAACTA 
GGAGACTCAG GAGTAAGAAA AATCTCCAGT CTACTGAGAA ACTCGCAGTG CATGCTGAAG 
ATACTCGGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTTACA TCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGGCTTACTA CTCGATCCAC ACTGTACACT GACGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAATTC TGTCAGTTTG TGTCTGGAAT TGTGGGTAAA 
TACCCATTAC TCCTGAAAGA ACTGAATCTG AGTGATCGTG GACTAGGGGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTTTAGAC TCAACACACT CATGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAA TTTAAATCCT 
TCACACCTGA TAGAGCTCAA TCTGAGTGGG AATAAACTAG AAGACTCAGG AGTAGAGAAG 
ATCTGTCCTC TGCTGGAAAA TATACAATGC CGATTAGAGA AACTGAGTCT TTCAGACTGC 
AGTATCAGGG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GATTTACTGC AGAGTAGAAG CTGTAAATTC AGGTTTCTGA AGAGTTCTGC TGCACAGGAA 
GCATGCGAGT ATCTTACTAA TGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT 
CTGAGTGAAG TTAAATTAGG AGATCTGGAC TGGGAGAAAC TCTCTGCTCT TCTGATGGAC 
TCTCATAGCA AAGTGGAGAA AATCAAGCTA AATAATTGTG AGCTGACAGA GAAAAGCTGT 
TCAGTTCTAG CTGCTGTTCT CTCCTCCAAA ACCATCCTGA AAGAGCTGAA CCTGAACAAC 
AGTCGTCTGC TGGACTCAGG AGTCAGAAAG ATCTGTGAGG GACTGAAGAA TCCTGTGTGT 
GAGCTGAAGA TACTCAATCT TTCAGACTGC AGTATTACAG AAGAAGGTTA TGAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GAGTTACTGC AGGATCCACA CTGTGCACTG 
AAGACACTGA GATTTTTAAG AAGTCCTGCT GCTGATGAAG GCTGTCAGTT AGTAACTGGA 
GTTGTGGGTA AAAACCCGTT ACTCCTGAAG GAACTGAATC TGAGTGATCA TGAGCTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGCCA GATCAACACA 
CTCAATCTGA GTTATTGCAG TATTACAGAG AAACAGTGTG AGATCCTGAC TTCAGCTCTG 
AAATCAAACC CATCACACCT GAGAGAACTG GACCTCAGTG GGAATGAACT AGGAGACTTT 
CAAAGCCTTG GCTCTCTACT GAAGAACCAA CAATTCAAGC TGGAGAAACT AATTCTGTGT 
GTTTGCAGTA TTAAAGAGAA GCAGTGTGAG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGAA CCTCAGTGGG AATGAACTCA AGCACAGAAG AGTGAAGCAA 
TTTTTTGATG TGCTGAAAAA ATCAACCTGT AAACTGGAGC GACTGAGGTT AAGATACTGT 
AGTATACAAG CCGATGATTG TACTGATCTG GCTTCAGCTC TGAAATCAAA CCCATCCCAC 
CTGAGAGAAC TGGACCTCAG TGGAAACAAT CTAGGAGACT CTGGAGTGAA AAAACTCAGT 
GATTTACTGA TAAACCAACA ATTCAAGCTG GAGAAACTAC GTCTGAGTTA TTGCAGTATT 
ACAGAGAAAC AGTGTGTGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA 
GAACTGAACC TCAATGGGAA TAGACTAGGA GACTCTGGAG CTGATGGCCT TAACCATCTG 
CTGAGCAGTG TGGATTGCAA GCTAAATAAA CTACATCTGT GTGATTGCAG TATTACAGAG 
AAACAGTTGC TCATCCCAAT CACAGCTACG TGTATGGGCC TATTACACCT GAGAGAACTG 
GACCTCAGTA AGAACCAAAT AAAAAACCAA GGAGTGTACA TGCTGTGTGA TGTGCTGAAA 
AACCCACGCT GTAGACTCAA GAGGTTAAGA CTAAATGACT GTGGCATTAC AGATGTTACA 
CCCTTAACTC AGTCTTTGAC CAAGGACAAA GCATTGAAGT TCTTAAAAGA GCTTGAACTG 
AGTAAAAATC AAATGGGATT CTTCGAAAAG CAAAAACTCA GTGACCTGCT GCGAGACTCA 
AACTGTAAAC TGAGGCTTGA GGAAGAAGAA GGTTATTTTA GAGCTGGTGT AAAACGGGTT 
ACTACATTTT TGATGCCTCC ATGGTCAAAA GTTCAAAAAC CTGTGGATCT CACAGGAGAG 
GAAAGTTCAT CATCAGATGA GGACGCCTCT GAAGATGAGA CTGATCAGGC AGAGGAGGCT 
GATAAGCTCT CAAATGAGGC GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_017353266.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208755.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X7, mRNA.

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CloneID	ODa57137
Clone ID Related Accession (Same CDS sequence)	XM_017353266.1 , XM_017353266.2
Accession Version	XM_017353266.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein si:ch211-11p18.6 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATA ATAATAATTT AAAGGATTCA GGAGTGAAGC TGCTTTGCAC TGGACTGAAG 
AACATAAAGT GTGAATTGGA AATACTGAGT CTTTCAGACT GCAGTATCAG TGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTAAGATCA AACCCTTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAGCTGA TAGAGTTACT GCAGGATCCA 
CTCTGTACAC TGAAGACACT GAGGTTTTTG AGTCCTGCTG CTGATGAAGC CTGTCAGTTT 
GTGACTGGAA TTGTGGGTAA AAACCTGTTA CTCCTGAAAG AACTGAATCT GAGTGATCGT 
CAACTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGTACA 
CTCAACACAC TCATGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTGACT 
TCAGCATTTA ATTTAAATCC ATCAAACCTG ATGGAGCTCA CTCTGAGTGG AAATAAACTA 
GAAGACTCAG GAGTAGAAAA GATCTGTTCT CTGCTGAAAA ATACACAGTG CAGATTGAAG 
AAACTGAGTC TTTCAGACTG CAGGATCACT GAAGAAGGAT ATAAAAGTTT GACTTTGTCC 
CTGAAGTCAA ATTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACAACGG GGTGATTGCT GTAAATTAAA AACCGTCAGG 
TTTTTAAAGA GTTCTGCTGC ACAGGAAGCG TGTGACTATC TTACTAAAGC TGTGGGTAAA 
AGTCCATTAT TACTGACAAA ACTGGATCTG AGTGAAGTTA AATTAGGAGA TCTGGATGGA 
GAGAAACTCT CTGCTCTTCT GAAGGACTCT CATAGCAAAG TGGAGAAAAT CAAGCTGAAT 
AATTGTGAGC TGACAGAGAA AAGCTGTTCA GTTCTAGCTG CTGTTCTCTC CTCCAAAACC 
ATCCTGAAAG AGATGAACCT GAACAACAGT CGTCTGCTGG ACTCAGGAGT CAGAGAGATC 
TGTGAGGGGC TGAAGAATCC TGTGTGTGAG CTGAAGATAC TCAACCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACTTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAC 
TTACTGCAGG ATCCACACTG TACCCTGAAT ACACTGAGGT TTTTGAGTCC TGCCGCAGAA 
GAAGCCTGTC AGTTTGTGAC TGGAATTGTG GGTAAAAACC CGATACTTCT GAAAGAACTG 
AATCTGAGTG ATCATGAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GTAAACTCAA CACACTGATT CTGAAAAACA GCTTTATCTC AGAGGAAGAT 
TGTCGTGTTC TGGCTGAAGC TTTTAATTCA AACCCATCAA ATCTGAGAGA GCTGAATTTG 
AATGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA ACACTCTGTT TCAAAACCAA 
CAGTGCAGAC TGGAAAAGCT GAAGTTTAGC GGCATCAGTA TCACAGCAGA AGGTTGTGCT 
GCTTTGACTT CAGCATTTAG CTCAAACCCG TCACACCTGA TAGAGCTAAA TCTGAGTGGG 
AATACACTAG AAGACTCAGG AGTAACAGAA ATCTCCAGTC TACTGGCAAA CTCACAGTGC 
ATGCTGAAGA TACTCAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GCTTTGATTC CAGAGTGTGC TCTGAAAACA 
TTGAGGTTTT TGGGTCCTGC AGCAGAAGAA GCCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCGT TACTCCTGAA AGAACTGAAT CTGAGTGATC GTAAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACCCAACAC ACTTCAGCTG 
AATAATAACA GGATTACAGC AGAAGGTTGT GCTGCTTTAA CTTCAGCATT TAATTTAAAC 
CCTTCACACC TAATAGAGCT CAATCTGAGT GGGAATAAAC TAAGAGACTC AGGAGTAGAG 
GAGATCTGTC CTCTGCTGAA AAATACACAG TGCAGACTGG AGAGACTAAG TCTTTCAGAC 
TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT AAAGGAGCTC 
ACTGACTTAC TGCAGGATGA GCGATATAGA TTGAAGACCC TCAGGCTTTT GAAAACTACT 
GCAGCACAAG AAGCGTGTGA CTATCTTACT AAAGTTGTGG GTAAAAGTCC ATTATTACTG 
ACAGAACTGG ATCTGAGTGA AGATAAATTA GGAGATCTGG ATGGAGAGAA ACTCTCTGCT 
CTTCTGATGG ACTCTCATAG TAAAGTGGAG AAAATCAAGC TGAATAATTG TAAGCAGATT 
GAAAAAGGCT GTTCAGTACT AGCTGCCGTT CTCTCCTCAA AAACAATCCT AAAAGAGATG 
AACCTGAACA ACAGTCGTCT GCTGGACTCA GGAGTCTTAG AGATCTGTGA GGGACTGAAG 
AACCCTGTGT GTGAGCTGAA GATTCTCAAG CTCAGTAAGT GTGATTTAAC AGAGGAAAGC 
TGTTCAGCTC TGGCTTCAGT TCTCAGATCA GACTCCAGCA GTCTGAAGGA TCTTGACCTG 
AGCAATAATA ATCTGCAGGA TTCAGGAGTG AAGTTGCTCT CTGATGGACT AAAGCACAGT 
AAACTGAAGA GACTCAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGC AGGATCCACA CTGTACACTA 
AAGACACTGA GTTGTTTTAG TCCTGCTGCT GATGAAGGCT GTCAGTTTGT TACTGGAATT 
GTGGGTAAAA ACCCGTTACT ACTGAAAGAA CTGAATCTGA GTGATCGTGA GCTAGGAGAC 
ACACGGGTGA ATCAACTCTC TGCTCTACTG CAAGATAAAC ACTGTACACT CAATATACTC 
ACGCTGAATA ATAACAGGAT TACAGCAGAA GGTTGTGCTG CTTTGACTTC AGCATTTAAT 
TTAAACCCTT CACACCTGAT AGAGCTCAAT CTGAGTGGAA ATAAACTAGG AGACTCAGGA 
GTAGAGAAGA TCTGTCCTCT GCTGAAAAAT ACCCAGTGCA GATTGGAGAA ATTAAATCTT 
TCAGACTGCA GCATCACTGG AAAAGGTTAT GAAACATTGA TTTCAGCTTT GAAGTCAAAC 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GGAAATCAGG AGTGCAGAAG 
CTCACTGATT TGCTACGAGC TGGAAGCTGT AAATTAAAAA CCATCAGGAC TTTGAAAGGT 
TCCGCTGCAC AGGAAGCGTG TGACTATCTA ACTAAAGCTG TGGGTAAAAG TCCATTATTA 
CTGACAGAAC TGGATCTGAG TGAAGATAAA TTAGGAGATC TGGATGGAGA GAAACTCTCT 
GCTCTTCTGA TGGACTCTCA TAGCAAAGTG GAGAAAATCA GGATGAATAA CTGTAAAAAG 
CTGACAGAGA AAAGCTGTTT AGTTCTAGCT GCTGTTCTTT CATCCAAAAC CATCTTGAAA 
GAGATGAACC TAAACAACAG TCGTCTGCTG GACTCAGGAG TCAAAGTGAT CTGTGAGGGA 
CTGAAGAACT CTAAGCTGGA GATTTTAAAA CTTTCAAATT GTAGTATTAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGAGATCA AACCCTTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCTGGAGTA AAGGAGCTCA CTGAGTTACT GCAGAGTTCG 
TGGATTCCAT GGAAAAAAGT GAAGTGTTCA ATGAGGTTTT TGAGTCCTGC TGCTGATGAA 
TTTTGTCAGT TTGTGTCTGG AATTGTGGGT AAAAACCCGT TACTCCTGAA AGAACTGAAT 
CTGAGTGATC ATGTACTAGC AGACACACAA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGCA AACTCAACAC ACTCATTCTT TCAGACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAA GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT GATGATGCAG ATGAAGCCTG TGAACATTTG 
TATAAAGTTC TGGGTGGAAA CCTGTTACTC CTGAGAGAGC TGAATCTGAC TAAACATGAT 
CTTGGACCAT CAGGATTAGA GAAACTTGCT GCAGTACTGA AAGATAAACA CTGTAAACTC 
AACACACTGA TTCTGAATAA CAGCACTATC TCAGAGGAAG ATTTTCATGT TCTGACTGAA 
GCTTTTAATT CAAACCCAAC AAATCTGACA GAGCTGAACT TGAGTGGGAC TAAACTCACA 
AACTCAGGAA TGAAGAGCTT CACAACTCTG TTTCAAAACC AACAGTGTAG ATTGGAAAAG 
CTGAAGTTCA GCAGCATGTG TATTACAGCA GAAGGTTGTG CTGCTTTGAC TTCAGCATTT 
AACTCAAACC CTTCACACCT GAAAGAGCTC AATCTGAGTG GGAATAAACT AGGAGACTCA 
GGAGTAAGAA AAATCTCCAG TCTACTGAGA AACTCGCAGT GCATGCTGAA GATACTCGGT 
CTTTCAGACT GCAGTATCAG TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTTAC ATCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACC ATCAGGAGTG 
AAGGAGCTCA CTGGCTTACT ACTCGATCCA CACTGTACAC TGACGACACT GAGGTTTTTG 
AGTCCTGCTG CTGATGAATT CTGTCAGTTT GTGTCTGGAA TTGTGGGTAA ATACCCATTA 
CTCCTGAAAG AACTGAATCT GAGTGATCGT GGACTAGGGG ACACACGAGT GAATCAACTC 
TCTGCTCTAC TGCAGGATAA ACACTTTAGA CTCAACACAC TCATGCTGAA TAATAACAGG 
ATTACAGCAG AAGGTTGTGC TGCTTTAACT TCAGCATTTA ATTTAAATCC TTCACACCTG 
ATAGAGCTCA ATCTGAGTGG GAATAAACTA GAAGACTCAG GAGTAGAGAA GATCTGTCCT 
CTGCTGGAAA ATATACAATG CCGATTAGAG AAACTGAGTC TTTCAGACTG CAGTATCAGG 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACTG 
CAGAGTAGAA GCTGTAAATT CAGGTTTCTG AAGAGTTCTG CTGCACAGGA AGCATGCGAG 
TATCTTACTA ATGTTCTGGG TATAAGTCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GTTAAATTAG GAGATCTGGA CTGGGAGAAA CTCTCTGCTC TTCTGATGGA CTCTCATAGC 
AAAGTGGAGA AAATCAAGCT AAATAATTGT GAGCTGACAG AGAAAAGCTG TTCAGTTCTA 
GCTGCTGTTC TCTCCTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAAA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAATC TTTCAGACTG CAGTATTACA GAAGAAGGTT ATGAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTGCACT GAAGACACTG 
AGATTTTTAA GAAGTCCTGC TGCTGATGAA GGCTGTCAGT TAGTAACTGG AGTTGTGGGT 
AAAAACCCGT TACTCCTGAA GGAACTGAAT CTGAGTGATC ATGAGCTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGCC AGATCAACAC ACTCAATCTG 
AGTTATTGCA GTATTACAGA GAAACAGTGT GAGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GGACCTCAGT GGGAATGAAC TAGGAGACTT TCAAAGCCTT 
GGCTCTCTAC TGAAGAACCA ACAATTCAAG CTGGAGAAAC TAATTCTGTG TGTTTGCAGT 
ATTAAAGAGA AGCAGTGTGA GATCCTGACT TCAGCTCTGA AATCAAACCC ATCACACCTG 
AGAGAACTGA ACCTCAGTGG GAATGAACTC AAGCACAGAA GAGTGAAGCA ATTTTTTGAT 
GTGCTGAAAA AATCAACCTG TAAACTGGAG CGACTGAGGT TAAGATACTG TAGTATACAA 
GCCGATGATT GTACTGATCT GGCTTCAGCT CTGAAATCAA ACCCATCCCA CCTGAGAGAA 
CTGGACCTCA GTGGAAACAA TCTAGGAGAC TCTGGAGTGA AAAAACTCAG TGATTTACTG 
ATAAACCAAC AATTCAAGCT GGAGAAACTA CGTCTGAGTT ATTGCAGTAT TACAGAGAAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAATGGGA ATAGACTAGG AGACTCTGGA GCTGATGGCC TTAACCATCT GCTGAGCAGT 
GTGGATTGCA AGCTAAATAA ACTACATCTG TGTGATTGCA GTATTACAGA GAAACAGTTG 
CTCATCCCAA TCACAGCTAC GTGTATGGGC CTATTACACC TGAGAGAACT GGACCTCAGT 
AAGAACCAAA TAAAAAACCA AGGAGTGTAC ATGCTGTGTG ATGTGCTGAA AAACCCACGC 
TGTAGACTCA AGAGGTTAAG ACTAAATGAC TGTGGCATTA CAGATGTTAC ACCCTTAACT 
CAGTCTTTGA CCAAGGACAA AGCATTGAAG TTCTTAAAAG AGCTTGAACT GAGTAAAAAT 
CAAATGGGAT TCTTCGAAAA GCAAAAACTC AGTGACCTGC TGCGAGACTC AAACTGTAAA 
CTGAGGCTTG AGGAAGAAGA AGGTTATTTT AGAGCTGGTG TAAAACGGGT TACTACATTT 
TTGATGCCTC CATGGTCAAA AGTTCAAAAA CCTGTGGATC TCACAGGAGA GGAAAGTTCA 
TCATCAGATG AGGACGCCTC TGAAGATGAG ACTGATCAGG CAGAGGAGGC TGATAAGCTC 
TCAAATGAGG CGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208755.1
CDS	81..7778
Translation	MNNNNNLKDSGVKLLCTGLKNIKCELEILSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELIELLQDPLCTLKTLRFLSPAADEACQFVTGIVGKNLLLLKELNLSDRQLGDTRVNQLSALLQDKHCTLNTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLKFSGISITAEGCAALTSAFSSNPSHLIELNLSGNTLEDSGVTEISSLLANSQCMLKILSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACEHLYKVLGGNLLLLRELNLTKHDLGPSGLEKLAAVLKDKHCKLNTLILNNSTISEEDFHVLTEAFNSNPTNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_017353266.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353266.1

ORF Insert Sequence:

ATGAATAATA ATAATAATTT AAAGGATTCA GGAGTGAAGC TGCTTTGCAC TGGACTGAAG 
AACATAAAGT GTGAATTGGA AATACTGAGT CTTTCAGACT GCAGTATCAG TGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTAAGATCA AACCCTTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAGCTGA TAGAGTTACT GCAGGATCCA 
CTCTGTACAC TGAAGACACT GAGGTTTTTG AGTCCTGCTG CTGATGAAGC CTGTCAGTTT 
GTGACTGGAA TTGTGGGTAA AAACCTGTTA CTCCTGAAAG AACTGAATCT GAGTGATCGT 
CAACTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGTACA 
CTCAACACAC TCATGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTGACT 
TCAGCATTTA ATTTAAATCC ATCAAACCTG ATGGAGCTCA CTCTGAGTGG AAATAAACTA 
GAAGACTCAG GAGTAGAAAA GATCTGTTCT CTGCTGAAAA ATACACAGTG CAGATTGAAG 
AAACTGAGTC TTTCAGACTG CAGGATCACT GAAGAAGGAT ATAAAAGTTT GACTTTGTCC 
CTGAAGTCAA ATTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACAACGG GGTGATTGCT GTAAATTAAA AACCGTCAGG 
TTTTTAAAGA GTTCTGCTGC ACAGGAAGCG TGTGACTATC TTACTAAAGC TGTGGGTAAA 
AGTCCATTAT TACTGACAAA ACTGGATCTG AGTGAAGTTA AATTAGGAGA TCTGGATGGA 
GAGAAACTCT CTGCTCTTCT GAAGGACTCT CATAGCAAAG TGGAGAAAAT CAAGCTGAAT 
AATTGTGAGC TGACAGAGAA AAGCTGTTCA GTTCTAGCTG CTGTTCTCTC CTCCAAAACC 
ATCCTGAAAG AGATGAACCT GAACAACAGT CGTCTGCTGG ACTCAGGAGT CAGAGAGATC 
TGTGAGGGGC TGAAGAATCC TGTGTGTGAG CTGAAGATAC TCAACCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACTTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAC 
TTACTGCAGG ATCCACACTG TACCCTGAAT ACACTGAGGT TTTTGAGTCC TGCCGCAGAA 
GAAGCCTGTC AGTTTGTGAC TGGAATTGTG GGTAAAAACC CGATACTTCT GAAAGAACTG 
AATCTGAGTG ATCATGAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GTAAACTCAA CACACTGATT CTGAAAAACA GCTTTATCTC AGAGGAAGAT 
TGTCGTGTTC TGGCTGAAGC TTTTAATTCA AACCCATCAA ATCTGAGAGA GCTGAATTTG 
AATGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA ACACTCTGTT TCAAAACCAA 
CAGTGCAGAC TGGAAAAGCT GAAGTTTAGC GGCATCAGTA TCACAGCAGA AGGTTGTGCT 
GCTTTGACTT CAGCATTTAG CTCAAACCCG TCACACCTGA TAGAGCTAAA TCTGAGTGGG 
AATACACTAG AAGACTCAGG AGTAACAGAA ATCTCCAGTC TACTGGCAAA CTCACAGTGC 
ATGCTGAAGA TACTCAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GCTTTGATTC CAGAGTGTGC TCTGAAAACA 
TTGAGGTTTT TGGGTCCTGC AGCAGAAGAA GCCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCGT TACTCCTGAA AGAACTGAAT CTGAGTGATC GTAAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACCCAACAC ACTTCAGCTG 
AATAATAACA GGATTACAGC AGAAGGTTGT GCTGCTTTAA CTTCAGCATT TAATTTAAAC 
CCTTCACACC TAATAGAGCT CAATCTGAGT GGGAATAAAC TAAGAGACTC AGGAGTAGAG 
GAGATCTGTC CTCTGCTGAA AAATACACAG TGCAGACTGG AGAGACTAAG TCTTTCAGAC 
TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT AAAGGAGCTC 
ACTGACTTAC TGCAGGATGA GCGATATAGA TTGAAGACCC TCAGGCTTTT GAAAACTACT 
GCAGCACAAG AAGCGTGTGA CTATCTTACT AAAGTTGTGG GTAAAAGTCC ATTATTACTG 
ACAGAACTGG ATCTGAGTGA AGATAAATTA GGAGATCTGG ATGGAGAGAA ACTCTCTGCT 
CTTCTGATGG ACTCTCATAG TAAAGTGGAG AAAATCAAGC TGAATAATTG TAAGCAGATT 
GAAAAAGGCT GTTCAGTACT AGCTGCCGTT CTCTCCTCAA AAACAATCCT AAAAGAGATG 
AACCTGAACA ACAGTCGTCT GCTGGACTCA GGAGTCTTAG AGATCTGTGA GGGACTGAAG 
AACCCTGTGT GTGAGCTGAA GATTCTCAAG CTCAGTAAGT GTGATTTAAC AGAGGAAAGC 
TGTTCAGCTC TGGCTTCAGT TCTCAGATCA GACTCCAGCA GTCTGAAGGA TCTTGACCTG 
AGCAATAATA ATCTGCAGGA TTCAGGAGTG AAGTTGCTCT CTGATGGACT AAAGCACAGT 
AAACTGAAGA GACTCAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGC AGGATCCACA CTGTACACTA 
AAGACACTGA GTTGTTTTAG TCCTGCTGCT GATGAAGGCT GTCAGTTTGT TACTGGAATT 
GTGGGTAAAA ACCCGTTACT ACTGAAAGAA CTGAATCTGA GTGATCGTGA GCTAGGAGAC 
ACACGGGTGA ATCAACTCTC TGCTCTACTG CAAGATAAAC ACTGTACACT CAATATACTC 
ACGCTGAATA ATAACAGGAT TACAGCAGAA GGTTGTGCTG CTTTGACTTC AGCATTTAAT 
TTAAACCCTT CACACCTGAT AGAGCTCAAT CTGAGTGGAA ATAAACTAGG AGACTCAGGA 
GTAGAGAAGA TCTGTCCTCT GCTGAAAAAT ACCCAGTGCA GATTGGAGAA ATTAAATCTT 
TCAGACTGCA GCATCACTGG AAAAGGTTAT GAAACATTGA TTTCAGCTTT GAAGTCAAAC 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GGAAATCAGG AGTGCAGAAG 
CTCACTGATT TGCTACGAGC TGGAAGCTGT AAATTAAAAA CCATCAGGAC TTTGAAAGGT 
TCCGCTGCAC AGGAAGCGTG TGACTATCTA ACTAAAGCTG TGGGTAAAAG TCCATTATTA 
CTGACAGAAC TGGATCTGAG TGAAGATAAA TTAGGAGATC TGGATGGAGA GAAACTCTCT 
GCTCTTCTGA TGGACTCTCA TAGCAAAGTG GAGAAAATCA GGATGAATAA CTGTAAAAAG 
CTGACAGAGA AAAGCTGTTT AGTTCTAGCT GCTGTTCTTT CATCCAAAAC CATCTTGAAA 
GAGATGAACC TAAACAACAG TCGTCTGCTG GACTCAGGAG TCAAAGTGAT CTGTGAGGGA 
CTGAAGAACT CTAAGCTGGA GATTTTAAAA CTTTCAAATT GTAGTATTAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGAGATCA AACCCTTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCTGGAGTA AAGGAGCTCA CTGAGTTACT GCAGAGTTCG 
TGGATTCCAT GGAAAAAAGT GAAGTGTTCA ATGAGGTTTT TGAGTCCTGC TGCTGATGAA 
TTTTGTCAGT TTGTGTCTGG AATTGTGGGT AAAAACCCGT TACTCCTGAA AGAACTGAAT 
CTGAGTGATC ATGTACTAGC AGACACACAA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGCA AACTCAACAC ACTCATTCTT TCAGACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAA GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT GATGATGCAG ATGAAGCCTG TGAACATTTG 
TATAAAGTTC TGGGTGGAAA CCTGTTACTC CTGAGAGAGC TGAATCTGAC TAAACATGAT 
CTTGGACCAT CAGGATTAGA GAAACTTGCT GCAGTACTGA AAGATAAACA CTGTAAACTC 
AACACACTGA TTCTGAATAA CAGCACTATC TCAGAGGAAG ATTTTCATGT TCTGACTGAA 
GCTTTTAATT CAAACCCAAC AAATCTGACA GAGCTGAACT TGAGTGGGAC TAAACTCACA 
AACTCAGGAA TGAAGAGCTT CACAACTCTG TTTCAAAACC AACAGTGTAG ATTGGAAAAG 
CTGAAGTTCA GCAGCATGTG TATTACAGCA GAAGGTTGTG CTGCTTTGAC TTCAGCATTT 
AACTCAAACC CTTCACACCT GAAAGAGCTC AATCTGAGTG GGAATAAACT AGGAGACTCA 
GGAGTAAGAA AAATCTCCAG TCTACTGAGA AACTCGCAGT GCATGCTGAA GATACTCGGT 
CTTTCAGACT GCAGTATCAG TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTTAC ATCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACC ATCAGGAGTG 
AAGGAGCTCA CTGGCTTACT ACTCGATCCA CACTGTACAC TGACGACACT GAGGTTTTTG 
AGTCCTGCTG CTGATGAATT CTGTCAGTTT GTGTCTGGAA TTGTGGGTAA ATACCCATTA 
CTCCTGAAAG AACTGAATCT GAGTGATCGT GGACTAGGGG ACACACGAGT GAATCAACTC 
TCTGCTCTAC TGCAGGATAA ACACTTTAGA CTCAACACAC TCATGCTGAA TAATAACAGG 
ATTACAGCAG AAGGTTGTGC TGCTTTAACT TCAGCATTTA ATTTAAATCC TTCACACCTG 
ATAGAGCTCA ATCTGAGTGG GAATAAACTA GAAGACTCAG GAGTAGAGAA GATCTGTCCT 
CTGCTGGAAA ATATACAATG CCGATTAGAG AAACTGAGTC TTTCAGACTG CAGTATCAGG 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACTG 
CAGAGTAGAA GCTGTAAATT CAGGTTTCTG AAGAGTTCTG CTGCACAGGA AGCATGCGAG 
TATCTTACTA ATGTTCTGGG TATAAGTCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GTTAAATTAG GAGATCTGGA CTGGGAGAAA CTCTCTGCTC TTCTGATGGA CTCTCATAGC 
AAAGTGGAGA AAATCAAGCT AAATAATTGT GAGCTGACAG AGAAAAGCTG TTCAGTTCTA 
GCTGCTGTTC TCTCCTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAAA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAATC TTTCAGACTG CAGTATTACA GAAGAAGGTT ATGAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTGCACT GAAGACACTG 
AGATTTTTAA GAAGTCCTGC TGCTGATGAA GGCTGTCAGT TAGTAACTGG AGTTGTGGGT 
AAAAACCCGT TACTCCTGAA GGAACTGAAT CTGAGTGATC ATGAGCTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGCC AGATCAACAC ACTCAATCTG 
AGTTATTGCA GTATTACAGA GAAACAGTGT GAGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GGACCTCAGT GGGAATGAAC TAGGAGACTT TCAAAGCCTT 
GGCTCTCTAC TGAAGAACCA ACAATTCAAG CTGGAGAAAC TAATTCTGTG TGTTTGCAGT 
ATTAAAGAGA AGCAGTGTGA GATCCTGACT TCAGCTCTGA AATCAAACCC ATCACACCTG 
AGAGAACTGA ACCTCAGTGG GAATGAACTC AAGCACAGAA GAGTGAAGCA ATTTTTTGAT 
GTGCTGAAAA AATCAACCTG TAAACTGGAG CGACTGAGGT TAAGATACTG TAGTATACAA 
GCCGATGATT GTACTGATCT GGCTTCAGCT CTGAAATCAA ACCCATCCCA CCTGAGAGAA 
CTGGACCTCA GTGGAAACAA TCTAGGAGAC TCTGGAGTGA AAAAACTCAG TGATTTACTG 
ATAAACCAAC AATTCAAGCT GGAGAAACTA CGTCTGAGTT ATTGCAGTAT TACAGAGAAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAATGGGA ATAGACTAGG AGACTCTGGA GCTGATGGCC TTAACCATCT GCTGAGCAGT 
GTGGATTGCA AGCTAAATAA ACTACATCTG TGTGATTGCA GTATTACAGA GAAACAGTTG 
CTCATCCCAA TCACAGCTAC GTGTATGGGC CTATTACACC TGAGAGAACT GGACCTCAGT 
AAGAACCAAA TAAAAAACCA AGGAGTGTAC ATGCTGTGTG ATGTGCTGAA AAACCCACGC 
TGTAGACTCA AGAGGTTAAG ACTAAATGAC TGTGGCATTA CAGATGTTAC ACCCTTAACT 
CAGTCTTTGA CCAAGGACAA AGCATTGAAG TTCTTAAAAG AGCTTGAACT GAGTAAAAAT 
CAAATGGGAT TCTTCGAAAA GCAAAAACTC AGTGACCTGC TGCGAGACTC AAACTGTAAA 
CTGAGGCTTG AGGAAGAAGA AGGTTATTTT AGAGCTGGTG TAAAACGGGT TACTACATTT 
TTGATGCCTC CATGGTCAAA AGTTCAAAAA CCTGTGGATC TCACAGGAGA GGAAAGTTCA 
TCATCAGATG AGGACGCCTC TGAAGATGAG ACTGATCAGG CAGAGGAGGC TGATAAGCTC 
TCAAATGAGG CGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_017353266.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208755.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X10, mRNA.

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CloneID	ODa57137
Clone ID Related Accession (Same CDS sequence)	XM_017353266.1 , XM_017353266.2
Accession Version	XM_017353266.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein si:ch211-11p18.6 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353266.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATA ATAATAATTT AAAGGATTCA GGAGTGAAGC TGCTTTGCAC TGGACTGAAG 
AACATAAAGT GTGAATTGGA AATACTGAGT CTTTCAGACT GCAGTATCAG TGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTAAGATCA AACCCTTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAGCTGA TAGAGTTACT GCAGGATCCA 
CTCTGTACAC TGAAGACACT GAGGTTTTTG AGTCCTGCTG CTGATGAAGC CTGTCAGTTT 
GTGACTGGAA TTGTGGGTAA AAACCTGTTA CTCCTGAAAG AACTGAATCT GAGTGATCGT 
CAACTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGTACA 
CTCAACACAC TCATGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTGACT 
TCAGCATTTA ATTTAAATCC ATCAAACCTG ATGGAGCTCA CTCTGAGTGG AAATAAACTA 
GAAGACTCAG GAGTAGAAAA GATCTGTTCT CTGCTGAAAA ATACACAGTG CAGATTGAAG 
AAACTGAGTC TTTCAGACTG CAGGATCACT GAAGAAGGAT ATAAAAGTTT GACTTTGTCC 
CTGAAGTCAA ATTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACAACGG GGTGATTGCT GTAAATTAAA AACCGTCAGG 
TTTTTAAAGA GTTCTGCTGC ACAGGAAGCG TGTGACTATC TTACTAAAGC TGTGGGTAAA 
AGTCCATTAT TACTGACAAA ACTGGATCTG AGTGAAGTTA AATTAGGAGA TCTGGATGGA 
GAGAAACTCT CTGCTCTTCT GAAGGACTCT CATAGCAAAG TGGAGAAAAT CAAGCTGAAT 
AATTGTGAGC TGACAGAGAA AAGCTGTTCA GTTCTAGCTG CTGTTCTCTC CTCCAAAACC 
ATCCTGAAAG AGATGAACCT GAACAACAGT CGTCTGCTGG ACTCAGGAGT CAGAGAGATC 
TGTGAGGGGC TGAAGAATCC TGTGTGTGAG CTGAAGATAC TCAACCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACTTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAC 
TTACTGCAGG ATCCACACTG TACCCTGAAT ACACTGAGGT TTTTGAGTCC TGCCGCAGAA 
GAAGCCTGTC AGTTTGTGAC TGGAATTGTG GGTAAAAACC CGATACTTCT GAAAGAACTG 
AATCTGAGTG ATCATGAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GTAAACTCAA CACACTGATT CTGAAAAACA GCTTTATCTC AGAGGAAGAT 
TGTCGTGTTC TGGCTGAAGC TTTTAATTCA AACCCATCAA ATCTGAGAGA GCTGAATTTG 
AATGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA ACACTCTGTT TCAAAACCAA 
CAGTGCAGAC TGGAAAAGCT GAAGTTTAGC GGCATCAGTA TCACAGCAGA AGGTTGTGCT 
GCTTTGACTT CAGCATTTAG CTCAAACCCG TCACACCTGA TAGAGCTAAA TCTGAGTGGG 
AATACACTAG AAGACTCAGG AGTAACAGAA ATCTCCAGTC TACTGGCAAA CTCACAGTGC 
ATGCTGAAGA TACTCAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GCTTTGATTC CAGAGTGTGC TCTGAAAACA 
TTGAGGTTTT TGGGTCCTGC AGCAGAAGAA GCCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCGT TACTCCTGAA AGAACTGAAT CTGAGTGATC GTAAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACCCAACAC ACTTCAGCTG 
AATAATAACA GGATTACAGC AGAAGGTTGT GCTGCTTTAA CTTCAGCATT TAATTTAAAC 
CCTTCACACC TAATAGAGCT CAATCTGAGT GGGAATAAAC TAAGAGACTC AGGAGTAGAG 
GAGATCTGTC CTCTGCTGAA AAATACACAG TGCAGACTGG AGAGACTAAG TCTTTCAGAC 
TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT AAAGGAGCTC 
ACTGACTTAC TGCAGGATGA GCGATATAGA TTGAAGACCC TCAGGCTTTT GAAAACTACT 
GCAGCACAAG AAGCGTGTGA CTATCTTACT AAAGTTGTGG GTAAAAGTCC ATTATTACTG 
ACAGAACTGG ATCTGAGTGA AGATAAATTA GGAGATCTGG ATGGAGAGAA ACTCTCTGCT 
CTTCTGATGG ACTCTCATAG TAAAGTGGAG AAAATCAAGC TGAATAATTG TAAGCAGATT 
GAAAAAGGCT GTTCAGTACT AGCTGCCGTT CTCTCCTCAA AAACAATCCT AAAAGAGATG 
AACCTGAACA ACAGTCGTCT GCTGGACTCA GGAGTCTTAG AGATCTGTGA GGGACTGAAG 
AACCCTGTGT GTGAGCTGAA GATTCTCAAG CTCAGTAAGT GTGATTTAAC AGAGGAAAGC 
TGTTCAGCTC TGGCTTCAGT TCTCAGATCA GACTCCAGCA GTCTGAAGGA TCTTGACCTG 
AGCAATAATA ATCTGCAGGA TTCAGGAGTG AAGTTGCTCT CTGATGGACT AAAGCACAGT 
AAACTGAAGA GACTCAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGC AGGATCCACA CTGTACACTA 
AAGACACTGA GTTGTTTTAG TCCTGCTGCT GATGAAGGCT GTCAGTTTGT TACTGGAATT 
GTGGGTAAAA ACCCGTTACT ACTGAAAGAA CTGAATCTGA GTGATCGTGA GCTAGGAGAC 
ACACGGGTGA ATCAACTCTC TGCTCTACTG CAAGATAAAC ACTGTACACT CAATATACTC 
ACGCTGAATA ATAACAGGAT TACAGCAGAA GGTTGTGCTG CTTTGACTTC AGCATTTAAT 
TTAAACCCTT CACACCTGAT AGAGCTCAAT CTGAGTGGAA ATAAACTAGG AGACTCAGGA 
GTAGAGAAGA TCTGTCCTCT GCTGAAAAAT ACCCAGTGCA GATTGGAGAA ATTAAATCTT 
TCAGACTGCA GCATCACTGG AAAAGGTTAT GAAACATTGA TTTCAGCTTT GAAGTCAAAC 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GGAAATCAGG AGTGCAGAAG 
CTCACTGATT TGCTACGAGC TGGAAGCTGT AAATTAAAAA CCATCAGGAC TTTGAAAGGT 
TCCGCTGCAC AGGAAGCGTG TGACTATCTA ACTAAAGCTG TGGGTAAAAG TCCATTATTA 
CTGACAGAAC TGGATCTGAG TGAAGATAAA TTAGGAGATC TGGATGGAGA GAAACTCTCT 
GCTCTTCTGA TGGACTCTCA TAGCAAAGTG GAGAAAATCA GGATGAATAA CTGTAAAAAG 
CTGACAGAGA AAAGCTGTTT AGTTCTAGCT GCTGTTCTTT CATCCAAAAC CATCTTGAAA 
GAGATGAACC TAAACAACAG TCGTCTGCTG GACTCAGGAG TCAAAGTGAT CTGTGAGGGA 
CTGAAGAACT CTAAGCTGGA GATTTTAAAA CTTTCAAATT GTAGTATTAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGAGATCA AACCCTTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCTGGAGTA AAGGAGCTCA CTGAGTTACT GCAGAGTTCG 
TGGATTCCAT GGAAAAAAGT GAAGTGTTCA ATGAGGTTTT TGAGTCCTGC TGCTGATGAA 
TTTTGTCAGT TTGTGTCTGG AATTGTGGGT AAAAACCCGT TACTCCTGAA AGAACTGAAT 
CTGAGTGATC ATGTACTAGC AGACACACAA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGCA AACTCAACAC ACTCATTCTT TCAGACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAA GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT GATGATGCAG ATGAAGCCTG TGAACATTTG 
TATAAAGTTC TGGGTGGAAA CCTGTTACTC CTGAGAGAGC TGAATCTGAC TAAACATGAT 
CTTGGACCAT CAGGATTAGA GAAACTTGCT GCAGTACTGA AAGATAAACA CTGTAAACTC 
AACACACTGA TTCTGAATAA CAGCACTATC TCAGAGGAAG ATTTTCATGT TCTGACTGAA 
GCTTTTAATT CAAACCCAAC AAATCTGACA GAGCTGAACT TGAGTGGGAC TAAACTCACA 
AACTCAGGAA TGAAGAGCTT CACAACTCTG TTTCAAAACC AACAGTGTAG ATTGGAAAAG 
CTGAAGTTCA GCAGCATGTG TATTACAGCA GAAGGTTGTG CTGCTTTGAC TTCAGCATTT 
AACTCAAACC CTTCACACCT GAAAGAGCTC AATCTGAGTG GGAATAAACT AGGAGACTCA 
GGAGTAAGAA AAATCTCCAG TCTACTGAGA AACTCGCAGT GCATGCTGAA GATACTCGGT 
CTTTCAGACT GCAGTATCAG TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTTAC ATCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACC ATCAGGAGTG 
AAGGAGCTCA CTGGCTTACT ACTCGATCCA CACTGTACAC TGACGACACT GAGGTTTTTG 
AGTCCTGCTG CTGATGAATT CTGTCAGTTT GTGTCTGGAA TTGTGGGTAA ATACCCATTA 
CTCCTGAAAG AACTGAATCT GAGTGATCGT GGACTAGGGG ACACACGAGT GAATCAACTC 
TCTGCTCTAC TGCAGGATAA ACACTTTAGA CTCAACACAC TCATGCTGAA TAATAACAGG 
ATTACAGCAG AAGGTTGTGC TGCTTTAACT TCAGCATTTA ATTTAAATCC TTCACACCTG 
ATAGAGCTCA ATCTGAGTGG GAATAAACTA GAAGACTCAG GAGTAGAGAA GATCTGTCCT 
CTGCTGGAAA ATATACAATG CCGATTAGAG AAACTGAGTC TTTCAGACTG CAGTATCAGG 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACTG 
CAGAGTAGAA GCTGTAAATT CAGGTTTCTG AAGAGTTCTG CTGCACAGGA AGCATGCGAG 
TATCTTACTA ATGTTCTGGG TATAAGTCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GTTAAATTAG GAGATCTGGA CTGGGAGAAA CTCTCTGCTC TTCTGATGGA CTCTCATAGC 
AAAGTGGAGA AAATCAAGCT AAATAATTGT GAGCTGACAG AGAAAAGCTG TTCAGTTCTA 
GCTGCTGTTC TCTCCTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAAA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAATC TTTCAGACTG CAGTATTACA GAAGAAGGTT ATGAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTGCACT GAAGACACTG 
AGATTTTTAA GAAGTCCTGC TGCTGATGAA GGCTGTCAGT TAGTAACTGG AGTTGTGGGT 
AAAAACCCGT TACTCCTGAA GGAACTGAAT CTGAGTGATC ATGAGCTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGCC AGATCAACAC ACTCAATCTG 
AGTTATTGCA GTATTACAGA GAAACAGTGT GAGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GGACCTCAGT GGGAATGAAC TAGGAGACTT TCAAAGCCTT 
GGCTCTCTAC TGAAGAACCA ACAATTCAAG CTGGAGAAAC TAATTCTGTG TGTTTGCAGT 
ATTAAAGAGA AGCAGTGTGA GATCCTGACT TCAGCTCTGA AATCAAACCC ATCACACCTG 
AGAGAACTGA ACCTCAGTGG GAATGAACTC AAGCACAGAA GAGTGAAGCA ATTTTTTGAT 
GTGCTGAAAA AATCAACCTG TAAACTGGAG CGACTGAGGT TAAGATACTG TAGTATACAA 
GCCGATGATT GTACTGATCT GGCTTCAGCT CTGAAATCAA ACCCATCCCA CCTGAGAGAA 
CTGGACCTCA GTGGAAACAA TCTAGGAGAC TCTGGAGTGA AAAAACTCAG TGATTTACTG 
ATAAACCAAC AATTCAAGCT GGAGAAACTA CGTCTGAGTT ATTGCAGTAT TACAGAGAAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAATGGGA ATAGACTAGG AGACTCTGGA GCTGATGGCC TTAACCATCT GCTGAGCAGT 
GTGGATTGCA AGCTAAATAA ACTACATCTG TGTGATTGCA GTATTACAGA GAAACAGTTG 
CTCATCCCAA TCACAGCTAC GTGTATGGGC CTATTACACC TGAGAGAACT GGACCTCAGT 
AAGAACCAAA TAAAAAACCA AGGAGTGTAC ATGCTGTGTG ATGTGCTGAA AAACCCACGC 
TGTAGACTCA AGAGGTTAAG ACTAAATGAC TGTGGCATTA CAGATGTTAC ACCCTTAACT 
CAGTCTTTGA CCAAGGACAA AGCATTGAAG TTCTTAAAAG AGCTTGAACT GAGTAAAAAT 
CAAATGGGAT TCTTCGAAAA GCAAAAACTC AGTGACCTGC TGCGAGACTC AAACTGTAAA 
CTGAGGCTTG AGGAAGAAGA AGGTTATTTT AGAGCTGGTG TAAAACGGGT TACTACATTT 
TTGATGCCTC CATGGTCAAA AGTTCAAAAA CCTGTGGATC TCACAGGAGA GGAAAGTTCA 
TCATCAGATG AGGACGCCTC TGAAGATGAG ACTGATCAGG CAGAGGAGGC TGATAAGCTC 
TCAAATGAGG CGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208755.1
CDS	80..7777
Translation	MNNNNNLKDSGVKLLCTGLKNIKCELEILSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELIELLQDPLCTLKTLRFLSPAADEACQFVTGIVGKNLLLLKELNLSDRQLGDTRVNQLSALLQDKHCTLNTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLKFSGISITAEGCAALTSAFSSNPSHLIELNLSGNTLEDSGVTEISSLLANSQCMLKILSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACEHLYKVLGGNLLLLRELNLTKHDLGPSGLEKLAAVLKDKHCKLNTLILNNSTISEEDFHVLTEAFNSNPTNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_017353266.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353266.2

ORF Insert Sequence:

ATGAATAATA ATAATAATTT AAAGGATTCA GGAGTGAAGC TGCTTTGCAC TGGACTGAAG 
AACATAAAGT GTGAATTGGA AATACTGAGT CTTTCAGACT GCAGTATCAG TGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTAAGATCA AACCCTTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAGCTGA TAGAGTTACT GCAGGATCCA 
CTCTGTACAC TGAAGACACT GAGGTTTTTG AGTCCTGCTG CTGATGAAGC CTGTCAGTTT 
GTGACTGGAA TTGTGGGTAA AAACCTGTTA CTCCTGAAAG AACTGAATCT GAGTGATCGT 
CAACTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGTACA 
CTCAACACAC TCATGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTGACT 
TCAGCATTTA ATTTAAATCC ATCAAACCTG ATGGAGCTCA CTCTGAGTGG AAATAAACTA 
GAAGACTCAG GAGTAGAAAA GATCTGTTCT CTGCTGAAAA ATACACAGTG CAGATTGAAG 
AAACTGAGTC TTTCAGACTG CAGGATCACT GAAGAAGGAT ATAAAAGTTT GACTTTGTCC 
CTGAAGTCAA ATTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACAACGG GGTGATTGCT GTAAATTAAA AACCGTCAGG 
TTTTTAAAGA GTTCTGCTGC ACAGGAAGCG TGTGACTATC TTACTAAAGC TGTGGGTAAA 
AGTCCATTAT TACTGACAAA ACTGGATCTG AGTGAAGTTA AATTAGGAGA TCTGGATGGA 
GAGAAACTCT CTGCTCTTCT GAAGGACTCT CATAGCAAAG TGGAGAAAAT CAAGCTGAAT 
AATTGTGAGC TGACAGAGAA AAGCTGTTCA GTTCTAGCTG CTGTTCTCTC CTCCAAAACC 
ATCCTGAAAG AGATGAACCT GAACAACAGT CGTCTGCTGG ACTCAGGAGT CAGAGAGATC 
TGTGAGGGGC TGAAGAATCC TGTGTGTGAG CTGAAGATAC TCAACCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACTTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAC 
TTACTGCAGG ATCCACACTG TACCCTGAAT ACACTGAGGT TTTTGAGTCC TGCCGCAGAA 
GAAGCCTGTC AGTTTGTGAC TGGAATTGTG GGTAAAAACC CGATACTTCT GAAAGAACTG 
AATCTGAGTG ATCATGAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GTAAACTCAA CACACTGATT CTGAAAAACA GCTTTATCTC AGAGGAAGAT 
TGTCGTGTTC TGGCTGAAGC TTTTAATTCA AACCCATCAA ATCTGAGAGA GCTGAATTTG 
AATGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA ACACTCTGTT TCAAAACCAA 
CAGTGCAGAC TGGAAAAGCT GAAGTTTAGC GGCATCAGTA TCACAGCAGA AGGTTGTGCT 
GCTTTGACTT CAGCATTTAG CTCAAACCCG TCACACCTGA TAGAGCTAAA TCTGAGTGGG 
AATACACTAG AAGACTCAGG AGTAACAGAA ATCTCCAGTC TACTGGCAAA CTCACAGTGC 
ATGCTGAAGA TACTCAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GCTTTGATTC CAGAGTGTGC TCTGAAAACA 
TTGAGGTTTT TGGGTCCTGC AGCAGAAGAA GCCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCGT TACTCCTGAA AGAACTGAAT CTGAGTGATC GTAAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACCCAACAC ACTTCAGCTG 
AATAATAACA GGATTACAGC AGAAGGTTGT GCTGCTTTAA CTTCAGCATT TAATTTAAAC 
CCTTCACACC TAATAGAGCT CAATCTGAGT GGGAATAAAC TAAGAGACTC AGGAGTAGAG 
GAGATCTGTC CTCTGCTGAA AAATACACAG TGCAGACTGG AGAGACTAAG TCTTTCAGAC 
TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT AAAGGAGCTC 
ACTGACTTAC TGCAGGATGA GCGATATAGA TTGAAGACCC TCAGGCTTTT GAAAACTACT 
GCAGCACAAG AAGCGTGTGA CTATCTTACT AAAGTTGTGG GTAAAAGTCC ATTATTACTG 
ACAGAACTGG ATCTGAGTGA AGATAAATTA GGAGATCTGG ATGGAGAGAA ACTCTCTGCT 
CTTCTGATGG ACTCTCATAG TAAAGTGGAG AAAATCAAGC TGAATAATTG TAAGCAGATT 
GAAAAAGGCT GTTCAGTACT AGCTGCCGTT CTCTCCTCAA AAACAATCCT AAAAGAGATG 
AACCTGAACA ACAGTCGTCT GCTGGACTCA GGAGTCTTAG AGATCTGTGA GGGACTGAAG 
AACCCTGTGT GTGAGCTGAA GATTCTCAAG CTCAGTAAGT GTGATTTAAC AGAGGAAAGC 
TGTTCAGCTC TGGCTTCAGT TCTCAGATCA GACTCCAGCA GTCTGAAGGA TCTTGACCTG 
AGCAATAATA ATCTGCAGGA TTCAGGAGTG AAGTTGCTCT CTGATGGACT AAAGCACAGT 
AAACTGAAGA GACTCAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGC AGGATCCACA CTGTACACTA 
AAGACACTGA GTTGTTTTAG TCCTGCTGCT GATGAAGGCT GTCAGTTTGT TACTGGAATT 
GTGGGTAAAA ACCCGTTACT ACTGAAAGAA CTGAATCTGA GTGATCGTGA GCTAGGAGAC 
ACACGGGTGA ATCAACTCTC TGCTCTACTG CAAGATAAAC ACTGTACACT CAATATACTC 
ACGCTGAATA ATAACAGGAT TACAGCAGAA GGTTGTGCTG CTTTGACTTC AGCATTTAAT 
TTAAACCCTT CACACCTGAT AGAGCTCAAT CTGAGTGGAA ATAAACTAGG AGACTCAGGA 
GTAGAGAAGA TCTGTCCTCT GCTGAAAAAT ACCCAGTGCA GATTGGAGAA ATTAAATCTT 
TCAGACTGCA GCATCACTGG AAAAGGTTAT GAAACATTGA TTTCAGCTTT GAAGTCAAAC 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GGAAATCAGG AGTGCAGAAG 
CTCACTGATT TGCTACGAGC TGGAAGCTGT AAATTAAAAA CCATCAGGAC TTTGAAAGGT 
TCCGCTGCAC AGGAAGCGTG TGACTATCTA ACTAAAGCTG TGGGTAAAAG TCCATTATTA 
CTGACAGAAC TGGATCTGAG TGAAGATAAA TTAGGAGATC TGGATGGAGA GAAACTCTCT 
GCTCTTCTGA TGGACTCTCA TAGCAAAGTG GAGAAAATCA GGATGAATAA CTGTAAAAAG 
CTGACAGAGA AAAGCTGTTT AGTTCTAGCT GCTGTTCTTT CATCCAAAAC CATCTTGAAA 
GAGATGAACC TAAACAACAG TCGTCTGCTG GACTCAGGAG TCAAAGTGAT CTGTGAGGGA 
CTGAAGAACT CTAAGCTGGA GATTTTAAAA CTTTCAAATT GTAGTATTAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGAGATCA AACCCTTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCTGGAGTA AAGGAGCTCA CTGAGTTACT GCAGAGTTCG 
TGGATTCCAT GGAAAAAAGT GAAGTGTTCA ATGAGGTTTT TGAGTCCTGC TGCTGATGAA 
TTTTGTCAGT TTGTGTCTGG AATTGTGGGT AAAAACCCGT TACTCCTGAA AGAACTGAAT 
CTGAGTGATC ATGTACTAGC AGACACACAA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGCA AACTCAACAC ACTCATTCTT TCAGACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAA GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT GATGATGCAG ATGAAGCCTG TGAACATTTG 
TATAAAGTTC TGGGTGGAAA CCTGTTACTC CTGAGAGAGC TGAATCTGAC TAAACATGAT 
CTTGGACCAT CAGGATTAGA GAAACTTGCT GCAGTACTGA AAGATAAACA CTGTAAACTC 
AACACACTGA TTCTGAATAA CAGCACTATC TCAGAGGAAG ATTTTCATGT TCTGACTGAA 
GCTTTTAATT CAAACCCAAC AAATCTGACA GAGCTGAACT TGAGTGGGAC TAAACTCACA 
AACTCAGGAA TGAAGAGCTT CACAACTCTG TTTCAAAACC AACAGTGTAG ATTGGAAAAG 
CTGAAGTTCA GCAGCATGTG TATTACAGCA GAAGGTTGTG CTGCTTTGAC TTCAGCATTT 
AACTCAAACC CTTCACACCT GAAAGAGCTC AATCTGAGTG GGAATAAACT AGGAGACTCA 
GGAGTAAGAA AAATCTCCAG TCTACTGAGA AACTCGCAGT GCATGCTGAA GATACTCGGT 
CTTTCAGACT GCAGTATCAG TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTTAC ATCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACC ATCAGGAGTG 
AAGGAGCTCA CTGGCTTACT ACTCGATCCA CACTGTACAC TGACGACACT GAGGTTTTTG 
AGTCCTGCTG CTGATGAATT CTGTCAGTTT GTGTCTGGAA TTGTGGGTAA ATACCCATTA 
CTCCTGAAAG AACTGAATCT GAGTGATCGT GGACTAGGGG ACACACGAGT GAATCAACTC 
TCTGCTCTAC TGCAGGATAA ACACTTTAGA CTCAACACAC TCATGCTGAA TAATAACAGG 
ATTACAGCAG AAGGTTGTGC TGCTTTAACT TCAGCATTTA ATTTAAATCC TTCACACCTG 
ATAGAGCTCA ATCTGAGTGG GAATAAACTA GAAGACTCAG GAGTAGAGAA GATCTGTCCT 
CTGCTGGAAA ATATACAATG CCGATTAGAG AAACTGAGTC TTTCAGACTG CAGTATCAGG 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACTG 
CAGAGTAGAA GCTGTAAATT CAGGTTTCTG AAGAGTTCTG CTGCACAGGA AGCATGCGAG 
TATCTTACTA ATGTTCTGGG TATAAGTCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GTTAAATTAG GAGATCTGGA CTGGGAGAAA CTCTCTGCTC TTCTGATGGA CTCTCATAGC 
AAAGTGGAGA AAATCAAGCT AAATAATTGT GAGCTGACAG AGAAAAGCTG TTCAGTTCTA 
GCTGCTGTTC TCTCCTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAAA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAATC TTTCAGACTG CAGTATTACA GAAGAAGGTT ATGAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTGCACT GAAGACACTG 
AGATTTTTAA GAAGTCCTGC TGCTGATGAA GGCTGTCAGT TAGTAACTGG AGTTGTGGGT 
AAAAACCCGT TACTCCTGAA GGAACTGAAT CTGAGTGATC ATGAGCTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGCC AGATCAACAC ACTCAATCTG 
AGTTATTGCA GTATTACAGA GAAACAGTGT GAGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GGACCTCAGT GGGAATGAAC TAGGAGACTT TCAAAGCCTT 
GGCTCTCTAC TGAAGAACCA ACAATTCAAG CTGGAGAAAC TAATTCTGTG TGTTTGCAGT 
ATTAAAGAGA AGCAGTGTGA GATCCTGACT TCAGCTCTGA AATCAAACCC ATCACACCTG 
AGAGAACTGA ACCTCAGTGG GAATGAACTC AAGCACAGAA GAGTGAAGCA ATTTTTTGAT 
GTGCTGAAAA AATCAACCTG TAAACTGGAG CGACTGAGGT TAAGATACTG TAGTATACAA 
GCCGATGATT GTACTGATCT GGCTTCAGCT CTGAAATCAA ACCCATCCCA CCTGAGAGAA 
CTGGACCTCA GTGGAAACAA TCTAGGAGAC TCTGGAGTGA AAAAACTCAG TGATTTACTG 
ATAAACCAAC AATTCAAGCT GGAGAAACTA CGTCTGAGTT ATTGCAGTAT TACAGAGAAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAATGGGA ATAGACTAGG AGACTCTGGA GCTGATGGCC TTAACCATCT GCTGAGCAGT 
GTGGATTGCA AGCTAAATAA ACTACATCTG TGTGATTGCA GTATTACAGA GAAACAGTTG 
CTCATCCCAA TCACAGCTAC GTGTATGGGC CTATTACACC TGAGAGAACT GGACCTCAGT 
AAGAACCAAA TAAAAAACCA AGGAGTGTAC ATGCTGTGTG ATGTGCTGAA AAACCCACGC 
TGTAGACTCA AGAGGTTAAG ACTAAATGAC TGTGGCATTA CAGATGTTAC ACCCTTAACT 
CAGTCTTTGA CCAAGGACAA AGCATTGAAG TTCTTAAAAG AGCTTGAACT GAGTAAAAAT 
CAAATGGGAT TCTTCGAAAA GCAAAAACTC AGTGACCTGC TGCGAGACTC AAACTGTAAA 
CTGAGGCTTG AGGAAGAAGA AGGTTATTTT AGAGCTGGTG TAAAACGGGT TACTACATTT 
TTGATGCCTC CATGGTCAAA AGTTCAAAAA CCTGTGGATC TCACAGGAGA GGAAAGTTCA 
TCATCAGATG AGGACGCCTC TGAAGATGAG ACTGATCAGG CAGAGGAGGC TGATAAGCTC 
TCAAATGAGG CGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_017353267.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208756.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X8, mRNA.

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CloneID	ODa57138
Clone ID Related Accession (Same CDS sequence)	XM_017353267.1
Accession Version	XM_017353267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	protein NLRC5 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAACGGGGTG ATTGCTGTAA ATTAAAAACC GTCAGGTTTT TAAAGAGTTC TGCTGCACAG 
GAAGCGTGTG ACTATCTTAC TAAAGCTGTG GGTAAAAGTC CATTATTACT GACAAAACTG 
GATCTGAGTG AAGTTAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGAAG 
GACTCTCATA GCAAAGTGGA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCAGTTC TAGCTGCTGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAGATCTGTG AGGGGCTGAA GAATCCTGTG 
TGTGAGCTGA AGATACTCAA CCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACTTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGACTTAC TGCAGGATCC ACACTGTACC 
CTGAATACAC TGAGGTTTTT GAGTCCTGCC GCAGAAGAAG CCTGTCAGTT TGTGACTGGA 
ATTGTGGGTA AAAACCCGAT ACTTCTGAAA GAACTGAATC TGAGTGATCA TGAACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGTAA ACTCAACACA 
CTGATTCTGA AAAACAGCTT TATCTCAGAG GAAGATTGTC GTGTTCTGGC TGAAGCTTTT 
AATTCAAACC CATCAAATCT GAGAGAGCTG AATTTGAATG GGACTAAACT CACAAACTCA 
GGAATGAAGA GCTTCAACAC TCTGTTTCAA AACCAACAGT GCAGACTGGA AAAGCTGAAG 
TTCAGCAGCA TGTGTATTAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAACTCA 
AACCCTTCAC ACCTGAAAGA GCTCAATCTG AGTGGGAATA AACTAGGAGA CTCAGGAGTA 
AGAAAAATCT CCAGTCTACT GAGAAACTCG CAGTGCATGC TGAAGATACT CGGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TTACATCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACCATCAGG AGTGAAGGAG 
CTCACTGGCT TACTACTCGA TCCACACTGT ACACTGACGA CACTGAGGTT TTTGAGTCCT 
GCTGCTGATG AATTCTGTCA GTTTGTGTCT GGAATTGTGG GTAAATACCC ATTACTCCTG 
AAAGAACTGA ATCTGAGTGA TCGTGGACTA GGGGACACAC GAGTGAATCA ACTCTCTGCT 
CTACTGCAGG ATAAACACTT TAGACTCAAC ACACTCATGC TGAATAATAA CAGGATTACA 
GCAGAAGGTT GTGCTGCTTT AACTTCAGCA TTTAATTTAA ATCCTTCACA CCTGATAGAG 
CTCAATCTGA GTGGGAATAA ACTAGAAGAC TCAGGAGTAG AGAAGATCTG TCCTCTGCTG 
GAAAATATAC AATGCCGATT AGAGAAACTG AGTCTTTCAG ACTGCAGTAT CAGGGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAGC TCACTGATTT ACTGCAGAGT 
AGAAGCTGTA AATTCAGGTT TCTGAAGAGT TCTGCTGCAC AGGAAGCATG CGAGTATCTT 
ACTAATGTTC TGGGTATAAG TCCATTATTA CTGACAGAAC TGGATCTGAG TGAAGTTAAA 
TTAGGAGATC TGGACTGGGA GAAACTCTCT GCTCTTCTGA TGGACTCTCA TAGCAAAGTG 
GAGAAAATCA AGCTAAATAA TTGTGAGCTG ACAGAGAAAA GCTGTTCAGT TCTAGCTGCT 
GTTCTCTCCT CCAAAACCAT CCTGAAAGAG CTGAACCTGA ACAACAGTCG TCTGCTGGAC 
TCAGGAGTCA GAAAGATCTG TGAGGGACTG AAGAATCCTG TGTGTGAGCT GAAGATACTC 
AATCTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATGAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTG CACTGAAGAC ACTGAGATTT 
TTAAGAAGTC CTGCTGCTGA TGAAGGCTGT CAGTTAGTAA CTGGAGTTGT GGGTAAAAAC 
CCGTTACTCC TGAAGGAACT GAATCTGAGT GATCATGAGC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGCCAGATCA ACACACTCAA TCTGAGTTAT 
TGCAGTATTA CAGAGAAACA GTGTGAGATC CTGACTTCAG CTCTGAAATC AAACCCATCA 
CACCTGAGAG AACTGGACCT CAGTGGGAAT GAACTAGGAG ACTTTCAAAG CCTTGGCTCT 
CTACTGAAGA ACCAACAATT CAAGCTGGAG AAACTAATTC TGTGTGTTTG CAGTATTAAA 
GAGAAGCAGT GTGAGATCCT GACTTCAGCT CTGAAATCAA ACCCATCACA CCTGAGAGAA 
CTGAACCTCA GTGGGAATGA ACTCAAGCAC AGAAGAGTGA AGCAATTTTT TGATGTGCTG 
AAAAAATCAA CCTGTAAACT GGAGCGACTG AGGTTAAGAT ACTGTAGTAT ACAAGCCGAT 
GATTGTACTG ATCTGGCTTC AGCTCTGAAA TCAAACCCAT CCCACCTGAG AGAACTGGAC 
CTCAGTGGAA ACAATCTAGG AGACTCTGGA GTGAAAAAAC TCAGTGATTT ACTGATAAAC 
CAACAATTCA AGCTGGAGAA ACTACGTCTG AGTTATTGCA GTATTACAGA GAAACAGTGT 
GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAAT 
GGGAATAGAC TAGGAGACTC TGGAGCTGAT GGCCTTAACC ATCTGCTGAG CAGTGTGGAT 
TGCAAGCTAA ATAAACTACA TCTGTGTGAT TGCAGTATTA CAGAGAAACA GTTGCTCATC 
CCAATCACAG CTACGTGTAT GGGCCTATTA CACCTGAGAG AACTGGACCT CAGTAAGAAC 
CAAATAAAAA ACCAAGGAGT GTACATGCTG TGTGATGTGC TGAAAAACCC ACGCTGTAGA 
CTCAAGAGGT TAAGACTAAA TGACTGTGGC ATTACAGATG TTACACCCTT AACTCAGTCT 
TTGACCAAGG ACAAAGCATT GAAGTTCTTA AAAGAGCTTG AACTGAGTAA AAATCAAATG 
GGATTCTTCG AAAAGCAAAA ACTCAGTGAC CTGCTGCGAG ACTCAAACTG TAAACTGAGG 
CTTGAGGAAG AAGAAGGTTA TTTTAGAGCT GGTGTAAAAC GGGTTACTAC ATTTTTGATG 
CCTCCATGGT CAAAAGTTCA AAAACCTGTG GATCTCACAG GAGAGGAAAG TTCATCATCA 
GATGAGGACG CCTCTGAAGA TGAGACTGAT CAGGCAGAGG AGGCTGATAA GCTCTCAAAT 
GAGGCGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208756.1
CDS	188..6919
Translation	MKMSLSEKHSVRSGSHVSSSVSVKSDRSKGHMPDFSETKTSPNKRFQYKTLEFDFQPQRSCNNFKDLQCIFQGLESKLFNFLKKELENFKKILQKENTQYLVKDFNENRCSIREAILDLTLYFLREMKQDEAADTLEDELIFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVASRRHEAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKKNREISFIFPLPFREMNLKEKETLSLMDLITQFFPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCCDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIERLTEIRGFNDAQKEEYFRKRLTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILQEKRNNGVKNNQADDASKTLQKSNTEDTPKTLTQMYTHFLRFQIQQSRRKYDGENTPDVSWDKDAILSLGKLAFHQLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVVGTMYCFVHLSVQEFIAALYAHLILDINKKSVFDQDSKNRNESMIDLLRTAVDKTLESDNGHLDLFLRFLLGLSLQSNRRLLQGLLTQQEHNDQSKEEIVQYIKQKFEVNHSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSADLSPAQWSALAFVLLTSEEEMEEFELQKFKKSDECLIRLSAVIKTCKRALLNDCNLTDRSCSTVAAVLRSENSLKELNMSNNNLQDSGVKLLCAGLKNIKCDLETLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSAAADEGCQFVTGIVGKNPLLLKELSLSGHELGDTRVNQLSALLQDKHCQINTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_017353267.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353267.1

ORF Insert Sequence:

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAACGGGGTG ATTGCTGTAA ATTAAAAACC GTCAGGTTTT TAAAGAGTTC TGCTGCACAG 
GAAGCGTGTG ACTATCTTAC TAAAGCTGTG GGTAAAAGTC CATTATTACT GACAAAACTG 
GATCTGAGTG AAGTTAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGAAG 
GACTCTCATA GCAAAGTGGA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCAGTTC TAGCTGCTGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAGATCTGTG AGGGGCTGAA GAATCCTGTG 
TGTGAGCTGA AGATACTCAA CCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACTTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGACTTAC TGCAGGATCC ACACTGTACC 
CTGAATACAC TGAGGTTTTT GAGTCCTGCC GCAGAAGAAG CCTGTCAGTT TGTGACTGGA 
ATTGTGGGTA AAAACCCGAT ACTTCTGAAA GAACTGAATC TGAGTGATCA TGAACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGTAA ACTCAACACA 
CTGATTCTGA AAAACAGCTT TATCTCAGAG GAAGATTGTC GTGTTCTGGC TGAAGCTTTT 
AATTCAAACC CATCAAATCT GAGAGAGCTG AATTTGAATG GGACTAAACT CACAAACTCA 
GGAATGAAGA GCTTCAACAC TCTGTTTCAA AACCAACAGT GCAGACTGGA AAAGCTGAAG 
TTCAGCAGCA TGTGTATTAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAACTCA 
AACCCTTCAC ACCTGAAAGA GCTCAATCTG AGTGGGAATA AACTAGGAGA CTCAGGAGTA 
AGAAAAATCT CCAGTCTACT GAGAAACTCG CAGTGCATGC TGAAGATACT CGGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TTACATCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACCATCAGG AGTGAAGGAG 
CTCACTGGCT TACTACTCGA TCCACACTGT ACACTGACGA CACTGAGGTT TTTGAGTCCT 
GCTGCTGATG AATTCTGTCA GTTTGTGTCT GGAATTGTGG GTAAATACCC ATTACTCCTG 
AAAGAACTGA ATCTGAGTGA TCGTGGACTA GGGGACACAC GAGTGAATCA ACTCTCTGCT 
CTACTGCAGG ATAAACACTT TAGACTCAAC ACACTCATGC TGAATAATAA CAGGATTACA 
GCAGAAGGTT GTGCTGCTTT AACTTCAGCA TTTAATTTAA ATCCTTCACA CCTGATAGAG 
CTCAATCTGA GTGGGAATAA ACTAGAAGAC TCAGGAGTAG AGAAGATCTG TCCTCTGCTG 
GAAAATATAC AATGCCGATT AGAGAAACTG AGTCTTTCAG ACTGCAGTAT CAGGGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAGC TCACTGATTT ACTGCAGAGT 
AGAAGCTGTA AATTCAGGTT TCTGAAGAGT TCTGCTGCAC AGGAAGCATG CGAGTATCTT 
ACTAATGTTC TGGGTATAAG TCCATTATTA CTGACAGAAC TGGATCTGAG TGAAGTTAAA 
TTAGGAGATC TGGACTGGGA GAAACTCTCT GCTCTTCTGA TGGACTCTCA TAGCAAAGTG 
GAGAAAATCA AGCTAAATAA TTGTGAGCTG ACAGAGAAAA GCTGTTCAGT TCTAGCTGCT 
GTTCTCTCCT CCAAAACCAT CCTGAAAGAG CTGAACCTGA ACAACAGTCG TCTGCTGGAC 
TCAGGAGTCA GAAAGATCTG TGAGGGACTG AAGAATCCTG TGTGTGAGCT GAAGATACTC 
AATCTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATGAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTG CACTGAAGAC ACTGAGATTT 
TTAAGAAGTC CTGCTGCTGA TGAAGGCTGT CAGTTAGTAA CTGGAGTTGT GGGTAAAAAC 
CCGTTACTCC TGAAGGAACT GAATCTGAGT GATCATGAGC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGCCAGATCA ACACACTCAA TCTGAGTTAT 
TGCAGTATTA CAGAGAAACA GTGTGAGATC CTGACTTCAG CTCTGAAATC AAACCCATCA 
CACCTGAGAG AACTGGACCT CAGTGGGAAT GAACTAGGAG ACTTTCAAAG CCTTGGCTCT 
CTACTGAAGA ACCAACAATT CAAGCTGGAG AAACTAATTC TGTGTGTTTG CAGTATTAAA 
GAGAAGCAGT GTGAGATCCT GACTTCAGCT CTGAAATCAA ACCCATCACA CCTGAGAGAA 
CTGAACCTCA GTGGGAATGA ACTCAAGCAC AGAAGAGTGA AGCAATTTTT TGATGTGCTG 
AAAAAATCAA CCTGTAAACT GGAGCGACTG AGGTTAAGAT ACTGTAGTAT ACAAGCCGAT 
GATTGTACTG ATCTGGCTTC AGCTCTGAAA TCAAACCCAT CCCACCTGAG AGAACTGGAC 
CTCAGTGGAA ACAATCTAGG AGACTCTGGA GTGAAAAAAC TCAGTGATTT ACTGATAAAC 
CAACAATTCA AGCTGGAGAA ACTACGTCTG AGTTATTGCA GTATTACAGA GAAACAGTGT 
GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAAT 
GGGAATAGAC TAGGAGACTC TGGAGCTGAT GGCCTTAACC ATCTGCTGAG CAGTGTGGAT 
TGCAAGCTAA ATAAACTACA TCTGTGTGAT TGCAGTATTA CAGAGAAACA GTTGCTCATC 
CCAATCACAG CTACGTGTAT GGGCCTATTA CACCTGAGAG AACTGGACCT CAGTAAGAAC 
CAAATAAAAA ACCAAGGAGT GTACATGCTG TGTGATGTGC TGAAAAACCC ACGCTGTAGA 
CTCAAGAGGT TAAGACTAAA TGACTGTGGC ATTACAGATG TTACACCCTT AACTCAGTCT 
TTGACCAAGG ACAAAGCATT GAAGTTCTTA AAAGAGCTTG AACTGAGTAA AAATCAAATG 
GGATTCTTCG AAAAGCAAAA ACTCAGTGAC CTGCTGCGAG ACTCAAACTG TAAACTGAGG 
CTTGAGGAAG AAGAAGGTTA TTTTAGAGCT GGTGTAAAAC GGGTTACTAC ATTTTTGATG 
CCTCCATGGT CAAAAGTTCA AAAACCTGTG GATCTCACAG GAGAGGAAAG TTCATCATCA 
GATGAGGACG CCTCTGAAGA TGAGACTGAT CAGGCAGAGG AGGCTGATAA GCTCTCAAAT 
GAGGCGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_017353268.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208757.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X9, mRNA.

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CloneID	ODa57139
Clone ID Related Accession (Same CDS sequence)	XM_017353268.1
Accession Version	XM_017353268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	protein NLRC5 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAACGGGGTG ATTGCTGTAA ATTAAAAACC GTCAGGTTTT TAAAGAGTTC TGCTGCACAG 
GAAGCGTGTG ACTATCTTAC TAAAGCTGTG GGTAAAAGTC CATTATTACT GACAAAACTG 
GATCTGAGTG AAGTTAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGAAG 
GACTCTCATA GCAAAGTGGA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCAGTTC TAGCTGCTGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAGATCTGTG AGGGGCTGAA GAATCCTGTG 
TGTGAGCTGA AGATACTCAA CCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACTTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGACTTAC TGCAGGATCC ACACTGTACC 
CTGAATACAC TGAGGTTTTT GAGTCCTGCC GCAGAAGAAG CCTGTCAGTT TGTGACTGGA 
ATTGTGGGTA AAAACCCGAT ACTTCTGAAA GAACTGAATC TGAGTGATCA TGAACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGTAA ACTCAACACA 
CTGATTCTGA AAAACAGCTT TATCTCAGAG GAAGATTGTC GTGTTCTGGC TGAAGCTTTT 
AATTCAAACC CATCAAATCT GAGAGAGCTG AATTTGAATG GGACTAAACT CACAAACTCA 
GGAATGAAGA GCTTCAACAC TCTGTTTCAA AACCAACAGT GCAGACTGGA AAAGCTGAAG 
TTTAGCGGCA TCAGTATCAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAGCTCA 
AACCCGTCAC ACCTGATAGA GCTAAATCTG AGTGGGAATA CACTAGAAGA CTCAGGAGTA 
ACAGAAATCT CCAGTCTACT GGCAAACTCA CAGTGCATGC TGAAGATACT CAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGCTT TGATTCCAGA GTGTGCTCTG AAAACATTGA GGTTTTTGGG TCCTGCAGCA 
GAAGAAGCCT GTCAGTTTGT GACTGGAATT GTGGGTAAAA ACCCGTTACT CCTGAAAGAA 
CTGAATCTGA GTGATCGTAA ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTACTG 
CAGGATAAAC ACTGTAAACC CAACACACTT CAGCTGAATA ATAACAGGAT TACAGCAGAA 
GGTTGTGCTG CTTTAACTTC AGCATTTAAT TTAAATCCTT CACACCTGAT AGAGCTCAAT 
CTGAGTGGGA ATAAACTAGA AGACTCAGGA GTAGAGAAGA TCTGTCCTCT GCTGGAAAAT 
ATACAATGCC GATTAGAGAA ACTGAGTCTT TCAGACTGCA GTATCAGGGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG ATTTACTGCA GAGTAGAAGC 
TGTAAATTCA GGTTTCTGAA GAGTTCTGCT GCACAGGAAG CATGCGAGTA TCTTACTAAT 
GTTCTGGGTA TAAGTCCATT ATTACTGACA GAACTGGATC TGAGTGAAGT TAAATTAGGA 
GATCTGGACT GGGAGAAACT CTCTGCTCTT CTGATGGACT CTCATAGCAA AGTGGAGAAA 
ATCAAGCTAA ATAATTGTGA GCTGACAGAG AAAAGCTGTT CAGTTCTAGC TGCTGTTCTC 
TCCTCCAAAA CCATCCTGAA AGAGCTGAAC CTGAACAACA GTCGTCTGCT GGACTCAGGA 
GTCAGAAAGA TCTGTGAGGG ACTGAAGAAT CCTGTGTGTG AGCTGAAGAT ACTCAATCTT 
TCAGACTGCA GTATTACAGA AGAAGGTTAT GAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG AGTTACTGCA GGATCCACAC TGTGCACTGA AGACACTGAG ATTTTTAAGA 
AGTCCTGCTG CTGATGAAGG CTGTCAGTTA GTAACTGGAG TTGTGGGTAA AAACCCGTTA 
CTCCTGAAGG AACTGAATCT GAGTGATCAT GAGCTAGGAG ACACACGAGT GAATCAACTC 
TCTGCTCTAC TGCAGGATAA ACACTGCCAG ATCAACACAC TCAATCTGAG TTATTGCAGT 
ATTACAGAGA AACAGTGTGA GATCCTGACT TCAGCTCTGA AATCAAACCC ATCACACCTG 
AGAGAACTGG ACCTCAGTGG GAATGAACTA GGAGACTTTC AAAGCCTTGG CTCTCTACTG 
AAGAACCAAC AATTCAAGCT GGAGAAACTA ATTCTGTGTG TTTGCAGTAT TAAAGAGAAG 
CAGTGTGAGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAGTGGGA ATGAACTCAA GCACAGAAGA GTGAAGCAAT TTTTTGATGT GCTGAAAAAA 
TCAACCTGTA AACTGGAGCG ACTGAGGTTA AGATACTGTA GTATACAAGC CGATGATTGT 
ACTGATCTGG CTTCAGCTCT GAAATCAAAC CCATCCCACC TGAGAGAACT GGACCTCAGT 
GGAAACAATC TAGGAGACTC TGGAGTGAAA AAACTCAGTG ATTTACTGAT AAACCAACAA 
TTCAAGCTGG AGAAACTACG TCTGAGTTAT TGCAGTATTA CAGAGAAACA GTGTGTGATC 
CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGAACCT CAATGGGAAT 
AGACTAGGAG ACTCTGGAGC TGATGGCCTT AACCATCTGC TGAGCAGTGT GGATTGCAAG 
CTAAATAAAC TACATCTGTG TGATTGCAGT ATTACAGAGA AACAGTTGCT CATCCCAATC 
ACAGCTACGT GTATGGGCCT ATTACACCTG AGAGAACTGG ACCTCAGTAA GAACCAAATA 
AAAAACCAAG GAGTGTACAT GCTGTGTGAT GTGCTGAAAA ACCCACGCTG TAGACTCAAG 
AGGTTAAGAC TAAATGACTG TGGCATTACA GATGTTACAC CCTTAACTCA GTCTTTGACC 
AAGGACAAAG CATTGAAGTT CTTAAAAGAG CTTGAACTGA GTAAAAATCA AATGGGATTC 
TTCGAAAAGC AAAAACTCAG TGACCTGCTG CGAGACTCAA ACTGTAAACT GAGGCTTGAG 
GAAGAAGAAG GTTATTTTAG AGCTGGTGTA AAACGGGTTA CTACATTTTT GATGCCTCCA 
TGGTCAAAAG TTCAAAAACC TGTGGATCTC ACAGGAGAGG AAAGTTCATC ATCAGATGAG 
GACGCCTCTG AAGATGAGAC TGATCAGGCA GAGGAGGCTG ATAAGCTCTC AAATGAGGCG 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208757.1
CDS	188..6913
Translation	MKMSLSEKHSVRSGSHVSSSVSVKSDRSKGHMPDFSETKTSPNKRFQYKTLEFDFQPQRSCNNFKDLQCIFQGLESKLFNFLKKELENFKKILQKENTQYLVKDFNENRCSIREAILDLTLYFLREMKQDEAADTLEDELIFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVASRRHEAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKKNREISFIFPLPFREMNLKEKETLSLMDLITQFFPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCCDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIERLTEIRGFNDAQKEEYFRKRLTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILQEKRNNGVKNNQADDASKTLQKSNTEDTPKTLTQMYTHFLRFQIQQSRRKYDGENTPDVSWDKDAILSLGKLAFHQLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVVGTMYCFVHLSVQEFIAALYAHLILDINKKSVFDQDSKNRNESMIDLLRTAVDKTLESDNGHLDLFLRFLLGLSLQSNRRLLQGLLTQQEHNDQSKEEIVQYIKQKFEVNHSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSADLSPAQWSALAFVLLTSEEEMEEFELQKFKKSDECLIRLSAVIKTCKRALLNDCNLTDRSCSTVAAVLRSENSLKELNMSNNNLQDSGVKLLCAGLKNIKCDLETLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSAAADEGCQFVTGIVGKNPLLLKELSLSGHELGDTRVNQLSALLQDKHCQINTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLKFSGISITAEGCAALTSAFSSNPSHLIELNLSGNTLEDSGVTEISSLLANSQCMLKILSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_017353268.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353268.1

ORF Insert Sequence:

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAACGGGGTG ATTGCTGTAA ATTAAAAACC GTCAGGTTTT TAAAGAGTTC TGCTGCACAG 
GAAGCGTGTG ACTATCTTAC TAAAGCTGTG GGTAAAAGTC CATTATTACT GACAAAACTG 
GATCTGAGTG AAGTTAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGAAG 
GACTCTCATA GCAAAGTGGA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCAGTTC TAGCTGCTGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAGATCTGTG AGGGGCTGAA GAATCCTGTG 
TGTGAGCTGA AGATACTCAA CCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACTTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGACTTAC TGCAGGATCC ACACTGTACC 
CTGAATACAC TGAGGTTTTT GAGTCCTGCC GCAGAAGAAG CCTGTCAGTT TGTGACTGGA 
ATTGTGGGTA AAAACCCGAT ACTTCTGAAA GAACTGAATC TGAGTGATCA TGAACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGTAA ACTCAACACA 
CTGATTCTGA AAAACAGCTT TATCTCAGAG GAAGATTGTC GTGTTCTGGC TGAAGCTTTT 
AATTCAAACC CATCAAATCT GAGAGAGCTG AATTTGAATG GGACTAAACT CACAAACTCA 
GGAATGAAGA GCTTCAACAC TCTGTTTCAA AACCAACAGT GCAGACTGGA AAAGCTGAAG 
TTTAGCGGCA TCAGTATCAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAGCTCA 
AACCCGTCAC ACCTGATAGA GCTAAATCTG AGTGGGAATA CACTAGAAGA CTCAGGAGTA 
ACAGAAATCT CCAGTCTACT GGCAAACTCA CAGTGCATGC TGAAGATACT CAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGCTT TGATTCCAGA GTGTGCTCTG AAAACATTGA GGTTTTTGGG TCCTGCAGCA 
GAAGAAGCCT GTCAGTTTGT GACTGGAATT GTGGGTAAAA ACCCGTTACT CCTGAAAGAA 
CTGAATCTGA GTGATCGTAA ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTACTG 
CAGGATAAAC ACTGTAAACC CAACACACTT CAGCTGAATA ATAACAGGAT TACAGCAGAA 
GGTTGTGCTG CTTTAACTTC AGCATTTAAT TTAAATCCTT CACACCTGAT AGAGCTCAAT 
CTGAGTGGGA ATAAACTAGA AGACTCAGGA GTAGAGAAGA TCTGTCCTCT GCTGGAAAAT 
ATACAATGCC GATTAGAGAA ACTGAGTCTT TCAGACTGCA GTATCAGGGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG ATTTACTGCA GAGTAGAAGC 
TGTAAATTCA GGTTTCTGAA GAGTTCTGCT GCACAGGAAG CATGCGAGTA TCTTACTAAT 
GTTCTGGGTA TAAGTCCATT ATTACTGACA GAACTGGATC TGAGTGAAGT TAAATTAGGA 
GATCTGGACT GGGAGAAACT CTCTGCTCTT CTGATGGACT CTCATAGCAA AGTGGAGAAA 
ATCAAGCTAA ATAATTGTGA GCTGACAGAG AAAAGCTGTT CAGTTCTAGC TGCTGTTCTC 
TCCTCCAAAA CCATCCTGAA AGAGCTGAAC CTGAACAACA GTCGTCTGCT GGACTCAGGA 
GTCAGAAAGA TCTGTGAGGG ACTGAAGAAT CCTGTGTGTG AGCTGAAGAT ACTCAATCTT 
TCAGACTGCA GTATTACAGA AGAAGGTTAT GAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG AGTTACTGCA GGATCCACAC TGTGCACTGA AGACACTGAG ATTTTTAAGA 
AGTCCTGCTG CTGATGAAGG CTGTCAGTTA GTAACTGGAG TTGTGGGTAA AAACCCGTTA 
CTCCTGAAGG AACTGAATCT GAGTGATCAT GAGCTAGGAG ACACACGAGT GAATCAACTC 
TCTGCTCTAC TGCAGGATAA ACACTGCCAG ATCAACACAC TCAATCTGAG TTATTGCAGT 
ATTACAGAGA AACAGTGTGA GATCCTGACT TCAGCTCTGA AATCAAACCC ATCACACCTG 
AGAGAACTGG ACCTCAGTGG GAATGAACTA GGAGACTTTC AAAGCCTTGG CTCTCTACTG 
AAGAACCAAC AATTCAAGCT GGAGAAACTA ATTCTGTGTG TTTGCAGTAT TAAAGAGAAG 
CAGTGTGAGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAGTGGGA ATGAACTCAA GCACAGAAGA GTGAAGCAAT TTTTTGATGT GCTGAAAAAA 
TCAACCTGTA AACTGGAGCG ACTGAGGTTA AGATACTGTA GTATACAAGC CGATGATTGT 
ACTGATCTGG CTTCAGCTCT GAAATCAAAC CCATCCCACC TGAGAGAACT GGACCTCAGT 
GGAAACAATC TAGGAGACTC TGGAGTGAAA AAACTCAGTG ATTTACTGAT AAACCAACAA 
TTCAAGCTGG AGAAACTACG TCTGAGTTAT TGCAGTATTA CAGAGAAACA GTGTGTGATC 
CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGAACCT CAATGGGAAT 
AGACTAGGAG ACTCTGGAGC TGATGGCCTT AACCATCTGC TGAGCAGTGT GGATTGCAAG 
CTAAATAAAC TACATCTGTG TGATTGCAGT ATTACAGAGA AACAGTTGCT CATCCCAATC 
ACAGCTACGT GTATGGGCCT ATTACACCTG AGAGAACTGG ACCTCAGTAA GAACCAAATA 
AAAAACCAAG GAGTGTACAT GCTGTGTGAT GTGCTGAAAA ACCCACGCTG TAGACTCAAG 
AGGTTAAGAC TAAATGACTG TGGCATTACA GATGTTACAC CCTTAACTCA GTCTTTGACC 
AAGGACAAAG CATTGAAGTT CTTAAAAGAG CTTGAACTGA GTAAAAATCA AATGGGATTC 
TTCGAAAAGC AAAAACTCAG TGACCTGCTG CGAGACTCAA ACTGTAAACT GAGGCTTGAG 
GAAGAAGAAG GTTATTTTAG AGCTGGTGTA AAACGGGTTA CTACATTTTT GATGCCTCCA 
TGGTCAAAAG TTCAAAAACC TGTGGATCTC ACAGGAGAGG AAAGTTCATC ATCAGATGAG 
GACGCCTCTG AAGATGAGAC TGATCAGGCA GAGGAGGCTG ATAAGCTCTC AAATGAGGCG 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_017353269.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208758.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X10, mRNA.

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CloneID	ODa57140
Clone ID Related Accession (Same CDS sequence)	XM_017353269.1
Accession Version	XM_017353269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	protein NLRC5 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAACGGGGTG ATTGCTGTAA ATTAAAAACC GTCAGGTTTT TAAAGAGTTC TGCTGCACAG 
GAAGCGTGTG ACTATCTTAC TAAAGCTGTG GGTAAAAGTC CATTATTACT GACAAAACTG 
GATCTGAGTG AAGTTAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGAAG 
GACTCTCATA GCAAAGTGGA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCAGTTC TAGCTGCTGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAGATCTGTG AGGGGCTGAA GAATCCTGTG 
TGTGAGCTGA AGATACTCAA CCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACTTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGACTTAC TGCAGGATCC ACACTGTACC 
CTGAATACAC TGAGGTTTTT GAGTCCTGCC GCAGAAGAAG CCTGTCAGTT TGTGACTGGA 
ATTGTGGGTA AAAACCCGAT ACTTCTGAAA GAACTGAATC TGAGTGATCA TGAACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGTAA ACTCAACACA 
CTGATTCTGA AAAACAGCTT TATCTCAGAG GAAGATTGTC GTGTTCTGGC TGAAGCTTTT 
AATTCAAACC CATCAAATCT GAGAGAGCTG AATTTGAATG GGACTAAACT CACAAACTCA 
GGAATGAAGA GCTTCAACAC TCTGTTTCAA AACCAACAGT GCAGACTGGA AAAGCTGAAC 
AGCATGTGTA TTACAGCAGA AGGTTGTGCT GCTTTGACTT CAGCATTTAA CTCAAACCCT 
TCACACCTGA AAGAGCTCAA TCTGAGTGGG AATAAACTAG GAGACTCAGG AGTAAGAAAA 
ATCTCCAGTC TACTGAGAAA CTCGCAGTGC ATGCTGAAGA TACTCGGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTTACAT 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACCAT CAGGAGTGAA GGAGCTCACT 
GGCTTACTAC TCGATCCACA CTGTACACTG ACGACACTGA GGTTTTTGAG TCCTGCTGCT 
GATGAATTCT GTCAGTTTGT GTCTGGAATT GTGGGTAAAT ACCCATTACT CCTGAAAGAA 
CTGAATCTGA GTGATCGTGG ACTAGGGGAC ACACGAGTGA ATCAACTCTC TGCTCTACTG 
CAGGATAAAC ACTTTAGACT CAACACACTC ATGCTGAATA ATAACAGGAT TACAGCAGAA 
GGTTGTGCTG CTTTAACTTC AGCATTTAAT TTAAATCCTT CACACCTGAT AGAGCTCAAT 
CTGAGTGGGA ATAAACTAGA AGACTCAGGA GTAGAGAAGA TCTGTCCTCT GCTGGAAAAT 
ATACAATGCC GATTAGAGAA ACTGAGTCTT TCAGACTGCA GTATCAGGGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG ATTTACTGCA GAGTAGAAGC 
TGTAAATTCA GGTTTCTGAA GAGTTCTGCT GCACAGGAAG CATGCGAGTA TCTTACTAAT 
GTTCTGGGTA TAAGTCCATT ATTACTGACA GAACTGGATC TGAGTGAAGT TAAATTAGGA 
GATCTGGACT GGGAGAAACT CTCTGCTCTT CTGATGGACT CTCATAGCAA AGTGGAGAAA 
ATCAAGCTAA ATAATTGTGA GCTGACAGAG AAAAGCTGTT CAGTTCTAGC TGCTGTTCTC 
TCCTCCAAAA CCATCCTGAA AGAGCTGAAC CTGAACAACA GTCGTCTGCT GGACTCAGGA 
GTCAGAAAGA TCTGTGAGGG ACTGAAGAAT CCTGTGTGTG AGCTGAAGAT ACTCAATCTT 
TCAGACTGCA GTATTACAGA AGAAGGTTAT GAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG AGTTACTGCA GGATCCACAC TGTGCACTGA AGACACTGAG ATTTTTAAGA 
AGTCCTGCTG CTGATGAAGG CTGTCAGTTA GTAACTGGAG TTGTGGGTAA AAACCCGTTA 
CTCCTGAAGG AACTGAATCT GAGTGATCAT GAGCTAGGAG ACACACGAGT GAATCAACTC 
TCTGCTCTAC TGCAGGATAA ACACTGCCAG ATCAACACAC TCAATCTGAG TTATTGCAGT 
ATTACAGAGA AACAGTGTGA GATCCTGACT TCAGCTCTGA AATCAAACCC ATCACACCTG 
AGAGAACTGG ACCTCAGTGG GAATGAACTA GGAGACTTTC AAAGCCTTGG CTCTCTACTG 
AAGAACCAAC AATTCAAGCT GGAGAAACTA ATTCTGTGTG TTTGCAGTAT TAAAGAGAAG 
CAGTGTGAGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAGTGGGA ATGAACTCAA GCACAGAAGA GTGAAGCAAT TTTTTGATGT GCTGAAAAAA 
TCAACCTGTA AACTGGAGCG ACTGAGGTTA AGATACTGTA GTATACAAGC CGATGATTGT 
ACTGATCTGG CTTCAGCTCT GAAATCAAAC CCATCCCACC TGAGAGAACT GGACCTCAGT 
GGAAACAATC TAGGAGACTC TGGAGTGAAA AAACTCAGTG ATTTACTGAT AAACCAACAA 
TTCAAGCTGG AGAAACTACG TCTGAGTTAT TGCAGTATTA CAGAGAAACA GTGTGTGATC 
CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGAACCT CAATGGGAAT 
AGACTAGGAG ACTCTGGAGC TGATGGCCTT AACCATCTGC TGAGCAGTGT GGATTGCAAG 
CTAAATAAAC TACATCTGTG TGATTGCAGT ATTACAGAGA AACAGTTGCT CATCCCAATC 
ACAGCTACGT GTATGGGCCT ATTACACCTG AGAGAACTGG ACCTCAGTAA GAACCAAATA 
AAAAACCAAG GAGTGTACAT GCTGTGTGAT GTGCTGAAAA ACCCACGCTG TAGACTCAAG 
AGGTTAAGAC TAAATGACTG TGGCATTACA GATGTTACAC CCTTAACTCA GTCTTTGACC 
AAGGACAAAG CATTGAAGTT CTTAAAAGAG CTTGAACTGA GTAAAAATCA AATGGGATTC 
TTCGAAAAGC AAAAACTCAG TGACCTGCTG CGAGACTCAA ACTGTAAACT GAGGCTTGAG 
GAAGAAGAAG GTTATTTTAG AGCTGGTGTA AAACGGGTTA CTACATTTTT GATGCCTCCA 
TGGTCAAAAG TTCAAAAACC TGTGGATCTC ACAGGAGAGG AAAGTTCATC ATCAGATGAG 
GACGCCTCTG AAGATGAGAC TGATCAGGCA GAGGAGGCTG ATAAGCTCTC AAATGAGGCG 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208758.1
CDS	188..6913
Translation	MKMSLSEKHSVRSGSHVSSSVSVKSDRSKGHMPDFSETKTSPNKRFQYKTLEFDFQPQRSCNNFKDLQCIFQGLESKLFNFLKKELENFKKILQKENTQYLVKDFNENRCSIREAILDLTLYFLREMKQDEAADTLEDELIFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVASRRHEAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKKNREISFIFPLPFREMNLKEKETLSLMDLITQFFPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCCDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIERLTEIRGFNDAQKEEYFRKRLTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILQEKRNNGVKNNQADDASKTLQKSNTEDTPKTLTQMYTHFLRFQIQQSRRKYDGENTPDVSWDKDAILSLGKLAFHQLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVVGTMYCFVHLSVQEFIAALYAHLILDINKKSVFDQDSKNRNESMIDLLRTAVDKTLESDNGHLDLFLRFLLGLSLQSNRRLLQGLLTQQEHNDQSKEEIVQYIKQKFEVNHSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSADLSPAQWSALAFVLLTSEEEMEEFELQKFKKSDECLIRLSAVIKTCKRALLNDCNLTDRSCSTVAAVLRSENSLKELNMSNNNLQDSGVKLLCAGLKNIKCDLETLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSAAADEGCQFVTGIVGKNPLLLKELSLSGHELGDTRVNQLSALLQDKHCQINTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLNSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_017353269.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353269.1

ORF Insert Sequence:

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAACGGGGTG ATTGCTGTAA ATTAAAAACC GTCAGGTTTT TAAAGAGTTC TGCTGCACAG 
GAAGCGTGTG ACTATCTTAC TAAAGCTGTG GGTAAAAGTC CATTATTACT GACAAAACTG 
GATCTGAGTG AAGTTAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGAAG 
GACTCTCATA GCAAAGTGGA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCAGTTC TAGCTGCTGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAGATCTGTG AGGGGCTGAA GAATCCTGTG 
TGTGAGCTGA AGATACTCAA CCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACTTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGACTTAC TGCAGGATCC ACACTGTACC 
CTGAATACAC TGAGGTTTTT GAGTCCTGCC GCAGAAGAAG CCTGTCAGTT TGTGACTGGA 
ATTGTGGGTA AAAACCCGAT ACTTCTGAAA GAACTGAATC TGAGTGATCA TGAACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGTAA ACTCAACACA 
CTGATTCTGA AAAACAGCTT TATCTCAGAG GAAGATTGTC GTGTTCTGGC TGAAGCTTTT 
AATTCAAACC CATCAAATCT GAGAGAGCTG AATTTGAATG GGACTAAACT CACAAACTCA 
GGAATGAAGA GCTTCAACAC TCTGTTTCAA AACCAACAGT GCAGACTGGA AAAGCTGAAC 
AGCATGTGTA TTACAGCAGA AGGTTGTGCT GCTTTGACTT CAGCATTTAA CTCAAACCCT 
TCACACCTGA AAGAGCTCAA TCTGAGTGGG AATAAACTAG GAGACTCAGG AGTAAGAAAA 
ATCTCCAGTC TACTGAGAAA CTCGCAGTGC ATGCTGAAGA TACTCGGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTTACAT 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACCAT CAGGAGTGAA GGAGCTCACT 
GGCTTACTAC TCGATCCACA CTGTACACTG ACGACACTGA GGTTTTTGAG TCCTGCTGCT 
GATGAATTCT GTCAGTTTGT GTCTGGAATT GTGGGTAAAT ACCCATTACT CCTGAAAGAA 
CTGAATCTGA GTGATCGTGG ACTAGGGGAC ACACGAGTGA ATCAACTCTC TGCTCTACTG 
CAGGATAAAC ACTTTAGACT CAACACACTC ATGCTGAATA ATAACAGGAT TACAGCAGAA 
GGTTGTGCTG CTTTAACTTC AGCATTTAAT TTAAATCCTT CACACCTGAT AGAGCTCAAT 
CTGAGTGGGA ATAAACTAGA AGACTCAGGA GTAGAGAAGA TCTGTCCTCT GCTGGAAAAT 
ATACAATGCC GATTAGAGAA ACTGAGTCTT TCAGACTGCA GTATCAGGGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG ATTTACTGCA GAGTAGAAGC 
TGTAAATTCA GGTTTCTGAA GAGTTCTGCT GCACAGGAAG CATGCGAGTA TCTTACTAAT 
GTTCTGGGTA TAAGTCCATT ATTACTGACA GAACTGGATC TGAGTGAAGT TAAATTAGGA 
GATCTGGACT GGGAGAAACT CTCTGCTCTT CTGATGGACT CTCATAGCAA AGTGGAGAAA 
ATCAAGCTAA ATAATTGTGA GCTGACAGAG AAAAGCTGTT CAGTTCTAGC TGCTGTTCTC 
TCCTCCAAAA CCATCCTGAA AGAGCTGAAC CTGAACAACA GTCGTCTGCT GGACTCAGGA 
GTCAGAAAGA TCTGTGAGGG ACTGAAGAAT CCTGTGTGTG AGCTGAAGAT ACTCAATCTT 
TCAGACTGCA GTATTACAGA AGAAGGTTAT GAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG AGTTACTGCA GGATCCACAC TGTGCACTGA AGACACTGAG ATTTTTAAGA 
AGTCCTGCTG CTGATGAAGG CTGTCAGTTA GTAACTGGAG TTGTGGGTAA AAACCCGTTA 
CTCCTGAAGG AACTGAATCT GAGTGATCAT GAGCTAGGAG ACACACGAGT GAATCAACTC 
TCTGCTCTAC TGCAGGATAA ACACTGCCAG ATCAACACAC TCAATCTGAG TTATTGCAGT 
ATTACAGAGA AACAGTGTGA GATCCTGACT TCAGCTCTGA AATCAAACCC ATCACACCTG 
AGAGAACTGG ACCTCAGTGG GAATGAACTA GGAGACTTTC AAAGCCTTGG CTCTCTACTG 
AAGAACCAAC AATTCAAGCT GGAGAAACTA ATTCTGTGTG TTTGCAGTAT TAAAGAGAAG 
CAGTGTGAGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAGTGGGA ATGAACTCAA GCACAGAAGA GTGAAGCAAT TTTTTGATGT GCTGAAAAAA 
TCAACCTGTA AACTGGAGCG ACTGAGGTTA AGATACTGTA GTATACAAGC CGATGATTGT 
ACTGATCTGG CTTCAGCTCT GAAATCAAAC CCATCCCACC TGAGAGAACT GGACCTCAGT 
GGAAACAATC TAGGAGACTC TGGAGTGAAA AAACTCAGTG ATTTACTGAT AAACCAACAA 
TTCAAGCTGG AGAAACTACG TCTGAGTTAT TGCAGTATTA CAGAGAAACA GTGTGTGATC 
CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGAACCT CAATGGGAAT 
AGACTAGGAG ACTCTGGAGC TGATGGCCTT AACCATCTGC TGAGCAGTGT GGATTGCAAG 
CTAAATAAAC TACATCTGTG TGATTGCAGT ATTACAGAGA AACAGTTGCT CATCCCAATC 
ACAGCTACGT GTATGGGCCT ATTACACCTG AGAGAACTGG ACCTCAGTAA GAACCAAATA 
AAAAACCAAG GAGTGTACAT GCTGTGTGAT GTGCTGAAAA ACCCACGCTG TAGACTCAAG 
AGGTTAAGAC TAAATGACTG TGGCATTACA GATGTTACAC CCTTAACTCA GTCTTTGACC 
AAGGACAAAG CATTGAAGTT CTTAAAAGAG CTTGAACTGA GTAAAAATCA AATGGGATTC 
TTCGAAAAGC AAAAACTCAG TGACCTGCTG CGAGACTCAA ACTGTAAACT GAGGCTTGAG 
GAAGAAGAAG GTTATTTTAG AGCTGGTGTA AAACGGGTTA CTACATTTTT GATGCCTCCA 
TGGTCAAAAG TTCAAAAACC TGTGGATCTC ACAGGAGAGG AAAGTTCATC ATCAGATGAG 
GACGCCTCTG AAGATGAGAC TGATCAGGCA GAGGAGGCTG ATAAGCTCTC AAATGAGGCG 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_017353270.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208759.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X11, mRNA.

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CloneID	ODa57141
Clone ID Related Accession (Same CDS sequence)	XM_017353270.1
Accession Version	XM_017353270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	protein NLRC5 isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAACGGGGTG ATTGCTGTAA ATTAAAAACC GTCAGGTTTT TAAAGAGTTC TGCTGCACAG 
GAAGCGTGTG ACTATCTTAC TAAAGCTGTG GGTAAAAGTC CATTATTACT GACAAAACTG 
GATCTGAGTG AAGTTAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGAAG 
GACTCTCATA GCAAAGTGGA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCAGTTC TAGCTGCTGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAGATCTGTG AGGGGCTGAA GAATCCTGTG 
TGTGAGCTGA AGATACTCAA CCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACTTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGACTTAC TGCAGGATCC ACACTGTACC 
CTGAATACAC TGAGGTTTTT GAGTCCTGCC GCAGAAGAAG CCTGTCAGTT TGTGACTGGA 
ATTGTGGGTA AAAACCCGAT ACTTCTGAAA GAACTGAATC TGAGTGATCA TGAACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGTAA ACTCAACACA 
CTGATTCTGA AAAACAGCTT TATCTCAGAG GAAGATTGTC GTGTTCTGGC TGAAGCTTTT 
AATTCAAACC CATCAAATCT GAGAGAGCTG AATTTGAATG GGACTAAACT CACAAACTCA 
GGAATGAAGA GCTTCAACAC TCTGTTTCAA AACCAACAGT GCAGACTGGA AAAGCTGAAC 
ATGTGTATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAACTC AAACCCTTCA 
CACCTGAAAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AAGAAAAATC 
TCCAGTCTAC TGAGAAACTC GCAGTGCATG CTGAAGATAC TCGGTCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTTACATCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACCATCAG GAGTGAAGGA GCTCACTGGC 
TTACTACTCG ATCCACACTG TACACTGACG ACACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAATTCTGTC AGTTTGTGTC TGGAATTGTG GGTAAATACC CATTACTCCT GAAAGAACTG 
AATCTGAGTG ATCGTGGACT AGGGGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT TTAGACTCAA CACACTCATG CTGAATAATA ACAGGATTAC AGCAGAAGGT 
TGTGCTGCTT TAACTTCAGC ATTTAATTTA AATCCTTCAC ACCTGATAGA GCTCAATCTG 
AGTGGGAATA AACTAGAAGA CTCAGGAGTA GAGAAGATCT GTCCTCTGCT GGAAAATATA 
CAATGCCGAT TAGAGAAACT GAGTCTTTCA GACTGCAGTA TCAGGGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGGAG CTCACTGATT TACTGCAGAG TAGAAGCTGT 
AAATTCAGGT TTCTGAAGAG TTCTGCTGCA CAGGAAGCAT GCGAGTATCT TACTAATGTT 
CTGGGTATAA GTCCATTATT ACTGACAGAA CTGGATCTGA GTGAAGTTAA ATTAGGAGAT 
CTGGACTGGG AGAAACTCTC TGCTCTTCTG ATGGACTCTC ATAGCAAAGT GGAGAAAATC 
AAGCTAAATA ATTGTGAGCT GACAGAGAAA AGCTGTTCAG TTCTAGCTGC TGTTCTCTCC 
TCCAAAACCA TCCTGAAAGA GCTGAACCTG AACAACAGTC GTCTGCTGGA CTCAGGAGTC 
AGAAAGATCT GTGAGGGACT GAAGAATCCT GTGTGTGAGC TGAAGATACT CAATCTTTCA 
GACTGCAGTA TTACAGAAGA AGGTTATGAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGAGT TACTGCAGGA TCCACACTGT GCACTGAAGA CACTGAGATT TTTAAGAAGT 
CCTGCTGCTG ATGAAGGCTG TCAGTTAGTA ACTGGAGTTG TGGGTAAAAA CCCGTTACTC 
CTGAAGGAAC TGAATCTGAG TGATCATGAG CTAGGAGACA CACGAGTGAA TCAACTCTCT 
GCTCTACTGC AGGATAAACA CTGCCAGATC AACACACTCA ATCTGAGTTA TTGCAGTATT 
ACAGAGAAAC AGTGTGAGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA 
GAACTGGACC TCAGTGGGAA TGAACTAGGA GACTTTCAAA GCCTTGGCTC TCTACTGAAG 
AACCAACAAT TCAAGCTGGA GAAACTAATT CTGTGTGTTT GCAGTATTAA AGAGAAGCAG 
TGTGAGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC 
AGTGGGAATG AACTCAAGCA CAGAAGAGTG AAGCAATTTT TTGATGTGCT GAAAAAATCA 
ACCTGTAAAC TGGAGCGACT GAGGTTAAGA TACTGTAGTA TACAAGCCGA TGATTGTACT 
GATCTGGCTT CAGCTCTGAA ATCAAACCCA TCCCACCTGA GAGAACTGGA CCTCAGTGGA 
AACAATCTAG GAGACTCTGG AGTGAAAAAA CTCAGTGATT TACTGATAAA CCAACAATTC 
AAGCTGGAGA AACTACGTCT GAGTTATTGC AGTATTACAG AGAAACAGTG TGTGATCCTG 
ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGAACCTCAA TGGGAATAGA 
CTAGGAGACT CTGGAGCTGA TGGCCTTAAC CATCTGCTGA GCAGTGTGGA TTGCAAGCTA 
AATAAACTAC ATCTGTGTGA TTGCAGTATT ACAGAGAAAC AGTTGCTCAT CCCAATCACA 
GCTACGTGTA TGGGCCTATT ACACCTGAGA GAACTGGACC TCAGTAAGAA CCAAATAAAA 
AACCAAGGAG TGTACATGCT GTGTGATGTG CTGAAAAACC CACGCTGTAG ACTCAAGAGG 
TTAAGACTAA ATGACTGTGG CATTACAGAT GTTACACCCT TAACTCAGTC TTTGACCAAG 
GACAAAGCAT TGAAGTTCTT AAAAGAGCTT GAACTGAGTA AAAATCAAAT GGGATTCTTC 
GAAAAGCAAA AACTCAGTGA CCTGCTGCGA GACTCAAACT GTAAACTGAG GCTTGAGGAA 
GAAGAAGGTT ATTTTAGAGC TGGTGTAAAA CGGGTTACTA CATTTTTGAT GCCTCCATGG 
TCAAAAGTTC AAAAACCTGT GGATCTCACA GGAGAGGAAA GTTCATCATC AGATGAGGAC 
GCCTCTGAAG ATGAGACTGA TCAGGCAGAG GAGGCTGATA AGCTCTCAAA TGAGGCGGAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208759.1
CDS	188..6910
Translation	MKMSLSEKHSVRSGSHVSSSVSVKSDRSKGHMPDFSETKTSPNKRFQYKTLEFDFQPQRSCNNFKDLQCIFQGLESKLFNFLKKELENFKKILQKENTQYLVKDFNENRCSIREAILDLTLYFLREMKQDEAADTLEDELIFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVASRRHEAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKKNREISFIFPLPFREMNLKEKETLSLMDLITQFFPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCCDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIERLTEIRGFNDAQKEEYFRKRLTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILQEKRNNGVKNNQADDASKTLQKSNTEDTPKTLTQMYTHFLRFQIQQSRRKYDGENTPDVSWDKDAILSLGKLAFHQLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVVGTMYCFVHLSVQEFIAALYAHLILDINKKSVFDQDSKNRNESMIDLLRTAVDKTLESDNGHLDLFLRFLLGLSLQSNRRLLQGLLTQQEHNDQSKEEIVQYIKQKFEVNHSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSADLSPAQWSALAFVLLTSEEEMEEFELQKFKKSDECLIRLSAVIKTCKRALLNDCNLTDRSCSTVAAVLRSENSLKELNMSNNNLQDSGVKLLCAGLKNIKCDLETLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSAAADEGCQFVTGIVGKNPLLLKELSLSGHELGDTRVNQLSALLQDKHCQINTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLNMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_017353270.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353270.1

ORF Insert Sequence:

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAACGGGGTG ATTGCTGTAA ATTAAAAACC GTCAGGTTTT TAAAGAGTTC TGCTGCACAG 
GAAGCGTGTG ACTATCTTAC TAAAGCTGTG GGTAAAAGTC CATTATTACT GACAAAACTG 
GATCTGAGTG AAGTTAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGAAG 
GACTCTCATA GCAAAGTGGA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCAGTTC TAGCTGCTGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAGATCTGTG AGGGGCTGAA GAATCCTGTG 
TGTGAGCTGA AGATACTCAA CCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACTTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGACTTAC TGCAGGATCC ACACTGTACC 
CTGAATACAC TGAGGTTTTT GAGTCCTGCC GCAGAAGAAG CCTGTCAGTT TGTGACTGGA 
ATTGTGGGTA AAAACCCGAT ACTTCTGAAA GAACTGAATC TGAGTGATCA TGAACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGTAA ACTCAACACA 
CTGATTCTGA AAAACAGCTT TATCTCAGAG GAAGATTGTC GTGTTCTGGC TGAAGCTTTT 
AATTCAAACC CATCAAATCT GAGAGAGCTG AATTTGAATG GGACTAAACT CACAAACTCA 
GGAATGAAGA GCTTCAACAC TCTGTTTCAA AACCAACAGT GCAGACTGGA AAAGCTGAAC 
ATGTGTATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAACTC AAACCCTTCA 
CACCTGAAAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AAGAAAAATC 
TCCAGTCTAC TGAGAAACTC GCAGTGCATG CTGAAGATAC TCGGTCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTTACATCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACCATCAG GAGTGAAGGA GCTCACTGGC 
TTACTACTCG ATCCACACTG TACACTGACG ACACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAATTCTGTC AGTTTGTGTC TGGAATTGTG GGTAAATACC CATTACTCCT GAAAGAACTG 
AATCTGAGTG ATCGTGGACT AGGGGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT TTAGACTCAA CACACTCATG CTGAATAATA ACAGGATTAC AGCAGAAGGT 
TGTGCTGCTT TAACTTCAGC ATTTAATTTA AATCCTTCAC ACCTGATAGA GCTCAATCTG 
AGTGGGAATA AACTAGAAGA CTCAGGAGTA GAGAAGATCT GTCCTCTGCT GGAAAATATA 
CAATGCCGAT TAGAGAAACT GAGTCTTTCA GACTGCAGTA TCAGGGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGGAG CTCACTGATT TACTGCAGAG TAGAAGCTGT 
AAATTCAGGT TTCTGAAGAG TTCTGCTGCA CAGGAAGCAT GCGAGTATCT TACTAATGTT 
CTGGGTATAA GTCCATTATT ACTGACAGAA CTGGATCTGA GTGAAGTTAA ATTAGGAGAT 
CTGGACTGGG AGAAACTCTC TGCTCTTCTG ATGGACTCTC ATAGCAAAGT GGAGAAAATC 
AAGCTAAATA ATTGTGAGCT GACAGAGAAA AGCTGTTCAG TTCTAGCTGC TGTTCTCTCC 
TCCAAAACCA TCCTGAAAGA GCTGAACCTG AACAACAGTC GTCTGCTGGA CTCAGGAGTC 
AGAAAGATCT GTGAGGGACT GAAGAATCCT GTGTGTGAGC TGAAGATACT CAATCTTTCA 
GACTGCAGTA TTACAGAAGA AGGTTATGAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGAGT TACTGCAGGA TCCACACTGT GCACTGAAGA CACTGAGATT TTTAAGAAGT 
CCTGCTGCTG ATGAAGGCTG TCAGTTAGTA ACTGGAGTTG TGGGTAAAAA CCCGTTACTC 
CTGAAGGAAC TGAATCTGAG TGATCATGAG CTAGGAGACA CACGAGTGAA TCAACTCTCT 
GCTCTACTGC AGGATAAACA CTGCCAGATC AACACACTCA ATCTGAGTTA TTGCAGTATT 
ACAGAGAAAC AGTGTGAGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA 
GAACTGGACC TCAGTGGGAA TGAACTAGGA GACTTTCAAA GCCTTGGCTC TCTACTGAAG 
AACCAACAAT TCAAGCTGGA GAAACTAATT CTGTGTGTTT GCAGTATTAA AGAGAAGCAG 
TGTGAGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC 
AGTGGGAATG AACTCAAGCA CAGAAGAGTG AAGCAATTTT TTGATGTGCT GAAAAAATCA 
ACCTGTAAAC TGGAGCGACT GAGGTTAAGA TACTGTAGTA TACAAGCCGA TGATTGTACT 
GATCTGGCTT CAGCTCTGAA ATCAAACCCA TCCCACCTGA GAGAACTGGA CCTCAGTGGA 
AACAATCTAG GAGACTCTGG AGTGAAAAAA CTCAGTGATT TACTGATAAA CCAACAATTC 
AAGCTGGAGA AACTACGTCT GAGTTATTGC AGTATTACAG AGAAACAGTG TGTGATCCTG 
ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGAACCTCAA TGGGAATAGA 
CTAGGAGACT CTGGAGCTGA TGGCCTTAAC CATCTGCTGA GCAGTGTGGA TTGCAAGCTA 
AATAAACTAC ATCTGTGTGA TTGCAGTATT ACAGAGAAAC AGTTGCTCAT CCCAATCACA 
GCTACGTGTA TGGGCCTATT ACACCTGAGA GAACTGGACC TCAGTAAGAA CCAAATAAAA 
AACCAAGGAG TGTACATGCT GTGTGATGTG CTGAAAAACC CACGCTGTAG ACTCAAGAGG 
TTAAGACTAA ATGACTGTGG CATTACAGAT GTTACACCCT TAACTCAGTC TTTGACCAAG 
GACAAAGCAT TGAAGTTCTT AAAAGAGCTT GAACTGAGTA AAAATCAAAT GGGATTCTTC 
GAAAAGCAAA AACTCAGTGA CCTGCTGCGA GACTCAAACT GTAAACTGAG GCTTGAGGAA 
GAAGAAGGTT ATTTTAGAGC TGGTGTAAAA CGGGTTACTA CATTTTTGAT GCCTCCATGG 
TCAAAAGTTC AAAAACCTGT GGATCTCACA GGAGAGGAAA GTTCATCATC AGATGAGGAC 
GCCTCTGAAG ATGAGACTGA TCAGGCAGAG GAGGCTGATA AGCTCTCAAA TGAGGCGGAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_017353271.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208760.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X12, mRNA.

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CloneID	ODa57142
Clone ID Related Accession (Same CDS sequence)	XM_017353271.1
Accession Version	XM_017353271.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	protein NLRC5 isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGAGTTA 
CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
TTTTGTCAGT TTGTGTCTGG AATTGTGGGT AAAAACCCGT TACTCCTGAA AGAACTGAAT 
CTGAGTGATC ATGTACTAGC AGACACACAA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGCA AACTCAACAC ACTCATTCTT TCAGACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAA GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT GATGATGCAG ATGAAGCCTG TGAACATTTG 
TATAAAGTTC TGGGTGGAAA CCTGTTACTC CTGAGAGAGC TGAATCTGAC TAAACATGAT 
CTTGGACCAT CAGGATTAGA GAAACTTGCT GCAGTACTGA AAGATAAACA CTGTAAACTC 
AACACACTGA TTCTGAATAA CAGCACTATC TCAGAGGAAG ATTTTCATGT TCTGACTGAA 
GCTTTTAATT CAAACCCAAC AAATCTGACA GAGCTGAACT TGAGTGGGAC TAAACTCACA 
AACTCAGGAA TGAAGAGCTT CACAACTCTG TTTCAAAACC AACAGTGTAG ATTGGAAAAG 
CTGAAGTTCA GCAGCATGTG TATTACAGCA GAAGGTTGTG CTGCTTTGAC TTCAGCATTT 
AACTCAAACC CTTCACACCT GAAAGAGCTC AATCTGAGTG GGAATAAACT AGGAGACTCA 
GGAGTAAGAA AAATCTCCAG TCTACTGAGA AACTCGCAGT GCATGCTGAA GATACTCGGT 
CTTTCAGACT GCAGTATCAG TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTTAC ATCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACC ATCAGGAGTG 
AAGGAGCTCA CTGGCTTACT ACTCGATCCA CACTGTACAC TGACGACACT GAGGTTTTTG 
AGTCCTGCTG CTGATGAATT CTGTCAGTTT GTGTCTGGAA TTGTGGGTAA ATACCCATTA 
CTCCTGAAAG AACTGAATCT GAGTGATCGT GGACTAGGGG ACACACGAGT GAATCAACTC 
TCTGCTCTAC TGCAGGATAA ACACTTTAGA CTCAACACAC TCATGCTGAA TAATAACAGG 
ATTACAGCAG AAGGTTGTGC TGCTTTAACT TCAGCATTTA ATTTAAATCC TTCACACCTG 
ATAGAGCTCA ATCTGAGTGG GAATAAACTA GAAGACTCAG GAGTAGAGAA GATCTGTCCT 
CTGCTGGAAA ATATACAATG CCGATTAGAG AAACTGAGTC TTTCAGACTG CAGTATCAGG 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACTG 
CAGAGTAGAA GCTGTAAATT CAGGTTTCTG AAGAGTTCTG CTGCACAGGA AGCATGCGAG 
TATCTTACTA ATGTTCTGGG TATAAGTCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GTTAAATTAG GAGATCTGGA CTGGGAGAAA CTCTCTGCTC TTCTGATGGA CTCTCATAGC 
AAAGTGGAGA AAATCAAGCT AAATAATTGT GAGCTGACAG AGAAAAGCTG TTCAGTTCTA 
GCTGCTGTTC TCTCCTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAAA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAATC TTTCAGACTG CAGTATTACA GAAGAAGGTT ATGAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTGCACT GAAGACACTG 
AGATTTTTAA GAAGTCCTGC TGCTGATGAA GGCTGTCAGT TAGTAACTGG AGTTGTGGGT 
AAAAACCCGT TACTCCTGAA GGAACTGAAT CTGAGTGATC ATGAGCTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGCC AGATCAACAC ACTCAATCTG 
AGTTATTGCA GTATTACAGA GAAACAGTGT GAGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GGACCTCAGT GGGAATGAAC TAGGAGACTT TCAAAGCCTT 
GGCTCTCTAC TGAAGAACCA ACAATTCAAG CTGGAGAAAC TAATTCTGTG TGTTTGCAGT 
ATTAAAGAGA AGCAGTGTGA GATCCTGACT TCAGCTCTGA AATCAAACCC ATCACACCTG 
AGAGAACTGA ACCTCAGTGG GAATGAACTC AAGCACAGAA GAGTGAAGCA ATTTTTTGAT 
GTGCTGAAAA AATCAACCTG TAAACTGGAG CGACTGAGGT TAAGATACTG TAGTATACAA 
GCCGATGATT GTACTGATCT GGCTTCAGCT CTGAAATCAA ACCCATCCCA CCTGAGAGAA 
CTGGACCTCA GTGGAAACAA TCTAGGAGAC TCTGGAGTGA AAAAACTCAG TGATTTACTG 
ATAAACCAAC AATTCAAGCT GGAGAAACTA CGTCTGAGTT ATTGCAGTAT TACAGAGAAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAATGGGA ATAGACTAGG AGACTCTGGA GCTGATGGCC TTAACCATCT GCTGAGCAGT 
GTGGATTGCA AGCTAAATAA ACTACATCTG TGTGATTGCA GTATTACAGA GAAACAGTTG 
CTCATCCCAA TCACAGCTAC GTGTATGGGC CTATTACACC TGAGAGAACT GGACCTCAGT 
AAGAACCAAA TAAAAAACCA AGGAGTGTAC ATGCTGTGTG ATGTGCTGAA AAACCCACGC 
TGTAGACTCA AGAGGTTAAG ACTAAATGAC TGTGGCATTA CAGATGTTAC ACCCTTAACT 
CAGTCTTTGA CCAAGGACAA AGCATTGAAG TTCTTAAAAG AGCTTGAACT GAGTAAAAAT 
CAAATGGGAT TCTTCGAAAA GCAAAAACTC AGTGACCTGC TGCGAGACTC AAACTGTAAA 
CTGAGGCTTG AGGAAGAAGA AGGTTATTTT AGAGCTGGTG TAAAACGGGT TACTACATTT 
TTGATGCCTC CATGGTCAAA AGTTCAAAAA CCTGTGGATC TCACAGGAGA GGAAAGTTCA 
TCATCAGATG AGGACGCCTC TGAAGATGAG ACTGATCAGG CAGAGGAGGC TGATAAGCTC 
TCAAATGAGG CGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208760.1
CDS	188..6745
Translation	MKMSLSEKHSVRSGSHVSSSVSVKSDRSKGHMPDFSETKTSPNKRFQYKTLEFDFQPQRSCNNFKDLQCIFQGLESKLFNFLKKELENFKKILQKENTQYLVKDFNENRCSIREAILDLTLYFLREMKQDEAADTLEDELIFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVASRRHEAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKKNREISFIFPLPFREMNLKEKETLSLMDLITQFFPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCCDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIERLTEIRGFNDAQKEEYFRKRLTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILQEKRNNGVKNNQADDASKTLQKSNTEDTPKTLTQMYTHFLRFQIQQSRRKYDGENTPDVSWDKDAILSLGKLAFHQLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVVGTMYCFVHLSVQEFIAALYAHLILDINKKSVFDQDSKNRNESMIDLLRTAVDKTLESDNGHLDLFLRFLLGLSLQSNRRLLQGLLTQQEHNDQSKEEIVQYIKQKFEVNHSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSADLSPAQWSALAFVLLTSEEEMEEFELQKFKKSDECLIRLSAVIKTCKRALLNDCNLTDRSCSTVAAVLRSENSLKELNMSNNNLQDSGVKLLCAGLKNIKCDLETLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSAAADEGCQFVTGIVGKNPLLLKELSLSGHELGDTRVNQLSALLQDKHCQINTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACEHLYKVLGGNLLLLRELNLTKHDLGPSGLEKLAAVLKDKHCKLNTLILNNSTISEEDFHVLTEAFNSNPTNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_017353271.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353271.1

ORF Insert Sequence:

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGAGTTA 
CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
TTTTGTCAGT TTGTGTCTGG AATTGTGGGT AAAAACCCGT TACTCCTGAA AGAACTGAAT 
CTGAGTGATC ATGTACTAGC AGACACACAA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGCA AACTCAACAC ACTCATTCTT TCAGACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAA GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT GATGATGCAG ATGAAGCCTG TGAACATTTG 
TATAAAGTTC TGGGTGGAAA CCTGTTACTC CTGAGAGAGC TGAATCTGAC TAAACATGAT 
CTTGGACCAT CAGGATTAGA GAAACTTGCT GCAGTACTGA AAGATAAACA CTGTAAACTC 
AACACACTGA TTCTGAATAA CAGCACTATC TCAGAGGAAG ATTTTCATGT TCTGACTGAA 
GCTTTTAATT CAAACCCAAC AAATCTGACA GAGCTGAACT TGAGTGGGAC TAAACTCACA 
AACTCAGGAA TGAAGAGCTT CACAACTCTG TTTCAAAACC AACAGTGTAG ATTGGAAAAG 
CTGAAGTTCA GCAGCATGTG TATTACAGCA GAAGGTTGTG CTGCTTTGAC TTCAGCATTT 
AACTCAAACC CTTCACACCT GAAAGAGCTC AATCTGAGTG GGAATAAACT AGGAGACTCA 
GGAGTAAGAA AAATCTCCAG TCTACTGAGA AACTCGCAGT GCATGCTGAA GATACTCGGT 
CTTTCAGACT GCAGTATCAG TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTTAC ATCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACC ATCAGGAGTG 
AAGGAGCTCA CTGGCTTACT ACTCGATCCA CACTGTACAC TGACGACACT GAGGTTTTTG 
AGTCCTGCTG CTGATGAATT CTGTCAGTTT GTGTCTGGAA TTGTGGGTAA ATACCCATTA 
CTCCTGAAAG AACTGAATCT GAGTGATCGT GGACTAGGGG ACACACGAGT GAATCAACTC 
TCTGCTCTAC TGCAGGATAA ACACTTTAGA CTCAACACAC TCATGCTGAA TAATAACAGG 
ATTACAGCAG AAGGTTGTGC TGCTTTAACT TCAGCATTTA ATTTAAATCC TTCACACCTG 
ATAGAGCTCA ATCTGAGTGG GAATAAACTA GAAGACTCAG GAGTAGAGAA GATCTGTCCT 
CTGCTGGAAA ATATACAATG CCGATTAGAG AAACTGAGTC TTTCAGACTG CAGTATCAGG 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACTG 
CAGAGTAGAA GCTGTAAATT CAGGTTTCTG AAGAGTTCTG CTGCACAGGA AGCATGCGAG 
TATCTTACTA ATGTTCTGGG TATAAGTCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GTTAAATTAG GAGATCTGGA CTGGGAGAAA CTCTCTGCTC TTCTGATGGA CTCTCATAGC 
AAAGTGGAGA AAATCAAGCT AAATAATTGT GAGCTGACAG AGAAAAGCTG TTCAGTTCTA 
GCTGCTGTTC TCTCCTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAAA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAATC TTTCAGACTG CAGTATTACA GAAGAAGGTT ATGAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTGCACT GAAGACACTG 
AGATTTTTAA GAAGTCCTGC TGCTGATGAA GGCTGTCAGT TAGTAACTGG AGTTGTGGGT 
AAAAACCCGT TACTCCTGAA GGAACTGAAT CTGAGTGATC ATGAGCTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGCC AGATCAACAC ACTCAATCTG 
AGTTATTGCA GTATTACAGA GAAACAGTGT GAGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GGACCTCAGT GGGAATGAAC TAGGAGACTT TCAAAGCCTT 
GGCTCTCTAC TGAAGAACCA ACAATTCAAG CTGGAGAAAC TAATTCTGTG TGTTTGCAGT 
ATTAAAGAGA AGCAGTGTGA GATCCTGACT TCAGCTCTGA AATCAAACCC ATCACACCTG 
AGAGAACTGA ACCTCAGTGG GAATGAACTC AAGCACAGAA GAGTGAAGCA ATTTTTTGAT 
GTGCTGAAAA AATCAACCTG TAAACTGGAG CGACTGAGGT TAAGATACTG TAGTATACAA 
GCCGATGATT GTACTGATCT GGCTTCAGCT CTGAAATCAA ACCCATCCCA CCTGAGAGAA 
CTGGACCTCA GTGGAAACAA TCTAGGAGAC TCTGGAGTGA AAAAACTCAG TGATTTACTG 
ATAAACCAAC AATTCAAGCT GGAGAAACTA CGTCTGAGTT ATTGCAGTAT TACAGAGAAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAATGGGA ATAGACTAGG AGACTCTGGA GCTGATGGCC TTAACCATCT GCTGAGCAGT 
GTGGATTGCA AGCTAAATAA ACTACATCTG TGTGATTGCA GTATTACAGA GAAACAGTTG 
CTCATCCCAA TCACAGCTAC GTGTATGGGC CTATTACACC TGAGAGAACT GGACCTCAGT 
AAGAACCAAA TAAAAAACCA AGGAGTGTAC ATGCTGTGTG ATGTGCTGAA AAACCCACGC 
TGTAGACTCA AGAGGTTAAG ACTAAATGAC TGTGGCATTA CAGATGTTAC ACCCTTAACT 
CAGTCTTTGA CCAAGGACAA AGCATTGAAG TTCTTAAAAG AGCTTGAACT GAGTAAAAAT 
CAAATGGGAT TCTTCGAAAA GCAAAAACTC AGTGACCTGC TGCGAGACTC AAACTGTAAA 
CTGAGGCTTG AGGAAGAAGA AGGTTATTTT AGAGCTGGTG TAAAACGGGT TACTACATTT 
TTGATGCCTC CATGGTCAAA AGTTCAAAAA CCTGTGGATC TCACAGGAGA GGAAAGTTCA 
TCATCAGATG AGGACGCCTC TGAAGATGAG ACTGATCAGG CAGAGGAGGC TGATAAGCTC 
TCAAATGAGG CGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_017353272.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208761.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X13, mRNA.

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CloneID	ODa57143
Clone ID Related Accession (Same CDS sequence)	XM_017353272.1
Accession Version	XM_017353272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	protein NLRC5 isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTGATGA TGCAGATGAA 
GCCTGTGAAC ATTTGTATAA AGTTCTGGGT GGAAACCTGT TACTCCTGAG AGAGCTGAAT 
CTGACTAAAC ATGATCTTGG ACCATCAGGA TTAGAGAAAC TTGCTGCAGT ACTGAAAGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AATAACAGCA CTATCTCAGA GGAAGATTTT 
CATGTTCTGA CTGAAGCTTT TAATTCAAAC CCAACAAATC TGACAGAGCT GAACTTGAGT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCACAA CTCTGTTTCA AAACCAACAG 
TGTAGATTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT 
TTGACTTCAG CATTTAACTC AAACCCTTCA CACCTGAAAG AGCTCAATCT GAGTGGGAAT 
AAACTAGGAG ACTCAGGAGT AAGAAAAATC TCCAGTCTAC TGAGAAACTC GCAGTGCATG 
CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTTACATCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACCATCAG GAGTGAAGGA GCTCACTGGC TTACTACTCG ATCCACACTG TACACTGACG 
ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GAATTCTGTC AGTTTGTGTC TGGAATTGTG 
GGTAAATACC CATTACTCCT GAAAGAACTG AATCTGAGTG ATCGTGGACT AGGGGACACA 
CGAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT TTAGACTCAA CACACTCATG 
CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TAACTTCAGC ATTTAATTTA 
AATCCTTCAC ACCTGATAGA GCTCAATCTG AGTGGGAATA AACTAGAAGA CTCAGGAGTA 
GAGAAGATCT GTCCTCTGCT GGAAAATATA CAATGCCGAT TAGAGAAACT GAGTCTTTCA 
GACTGCAGTA TCAGGGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGATT TACTGCAGAG TAGAAGCTGT AAATTCAGGT TTCTGAAGAG TTCTGCTGCA 
CAGGAAGCAT GCGAGTATCT TACTAATGTT CTGGGTATAA GTCCATTATT ACTGACAGAA 
CTGGATCTGA GTGAAGTTAA ATTAGGAGAT CTGGACTGGG AGAAACTCTC TGCTCTTCTG 
ATGGACTCTC ATAGCAAAGT GGAGAAAATC AAGCTAAATA ATTGTGAGCT GACAGAGAAA 
AGCTGTTCAG TTCTAGCTGC TGTTCTCTCC TCCAAAACCA TCCTGAAAGA GCTGAACCTG 
AACAACAGTC GTCTGCTGGA CTCAGGAGTC AGAAAGATCT GTGAGGGACT GAAGAATCCT 
GTGTGTGAGC TGAAGATACT CAATCTTTCA GACTGCAGTA TTACAGAAGA AGGTTATGAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGGAG CTCACTGAGT TACTGCAGGA TCCACACTGT 
GCACTGAAGA CACTGAGATT TTTAAGAAGT CCTGCTGCTG ATGAAGGCTG TCAGTTAGTA 
ACTGGAGTTG TGGGTAAAAA CCCGTTACTC CTGAAGGAAC TGAATCTGAG TGATCATGAG 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGCCAGATC 
AACACACTCA ATCTGAGTTA TTGCAGTATT ACAGAGAAAC AGTGTGAGAT CCTGACTTCA 
GCTCTGAAAT CAAACCCATC ACACCTGAGA GAACTGGACC TCAGTGGGAA TGAACTAGGA 
GACTTTCAAA GCCTTGGCTC TCTACTGAAG AACCAACAAT TCAAGCTGGA GAAACTAATT 
CTGTGTGTTT GCAGTATTAA AGAGAAGCAG TGTGAGATCC TGACTTCAGC TCTGAAATCA 
AACCCATCAC ACCTGAGAGA ACTGAACCTC AGTGGGAATG AACTCAAGCA CAGAAGAGTG 
AAGCAATTTT TTGATGTGCT GAAAAAATCA ACCTGTAAAC TGGAGCGACT GAGGTTAAGA 
TACTGTAGTA TACAAGCCGA TGATTGTACT GATCTGGCTT CAGCTCTGAA ATCAAACCCA 
TCCCACCTGA GAGAACTGGA CCTCAGTGGA AACAATCTAG GAGACTCTGG AGTGAAAAAA 
CTCAGTGATT TACTGATAAA CCAACAATTC AAGCTGGAGA AACTACGTCT GAGTTATTGC 
AGTATTACAG AGAAACAGTG TGTGATCCTG ACTTCAGCTC TGAAATCAAA CCCATCACAC 
CTGAGAGAAC TGAACCTCAA TGGGAATAGA CTAGGAGACT CTGGAGCTGA TGGCCTTAAC 
CATCTGCTGA GCAGTGTGGA TTGCAAGCTA AATAAACTAC ATCTGTGTGA TTGCAGTATT 
ACAGAGAAAC AGTTGCTCAT CCCAATCACA GCTACGTGTA TGGGCCTATT ACACCTGAGA 
GAACTGGACC TCAGTAAGAA CCAAATAAAA AACCAAGGAG TGTACATGCT GTGTGATGTG 
CTGAAAAACC CACGCTGTAG ACTCAAGAGG TTAAGACTAA ATGACTGTGG CATTACAGAT 
GTTACACCCT TAACTCAGTC TTTGACCAAG GACAAAGCAT TGAAGTTCTT AAAAGAGCTT 
GAACTGAGTA AAAATCAAAT GGGATTCTTC GAAAAGCAAA AACTCAGTGA CCTGCTGCGA 
GACTCAAACT GTAAACTGAG GCTTGAGGAA GAAGAAGGTT ATTTTAGAGC TGGTGTAAAA 
CGGGTTACTA CATTTTTGAT GCCTCCATGG TCAAAAGTTC AAAAACCTGT GGATCTCACA 
GGAGAGGAAA GTTCATCATC AGATGAGGAC GCCTCTGAAG ATGAGACTGA TCAGGCAGAG 
GAGGCTGATA AGCTCTCAAA TGAGGCGGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208761.1
CDS	188..6400
Translation	MKMSLSEKHSVRSGSHVSSSVSVKSDRSKGHMPDFSETKTSPNKRFQYKTLEFDFQPQRSCNNFKDLQCIFQGLESKLFNFLKKELENFKKILQKENTQYLVKDFNENRCSIREAILDLTLYFLREMKQDEAADTLEDELIFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVASRRHEAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKKNREISFIFPLPFREMNLKEKETLSLMDLITQFFPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCCDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIERLTEIRGFNDAQKEEYFRKRLTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILQEKRNNGVKNNQADDASKTLQKSNTEDTPKTLTQMYTHFLRFQIQQSRRKYDGENTPDVSWDKDAILSLGKLAFHQLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVVGTMYCFVHLSVQEFIAALYAHLILDINKKSVFDQDSKNRNESMIDLLRTAVDKTLESDNGHLDLFLRFLLGLSLQSNRRLLQGLLTQQEHNDQSKEEIVQYIKQKFEVNHSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSADLSPAQWSALAFVLLTSEEEMEEFELQKFKKSDECLIRLSAVIKTCKRALLNDCNLTDRSCSTVAAVLRSENSLKELNMSNNNLQDSGVKLLCAGLKNIKCDLETLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSAAADEGCQFVTGIVGKNPLLLKELSLSGHELGDTRVNQLSALLQDKHCQINTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSDDADEACEHLYKVLGGNLLLLRELNLTKHDLGPSGLEKLAAVLKDKHCKLNTLILNNSTISEEDFHVLTEAFNSNPTNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_017353272.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353272.1

ORF Insert Sequence:

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTGATGA TGCAGATGAA 
GCCTGTGAAC ATTTGTATAA AGTTCTGGGT GGAAACCTGT TACTCCTGAG AGAGCTGAAT 
CTGACTAAAC ATGATCTTGG ACCATCAGGA TTAGAGAAAC TTGCTGCAGT ACTGAAAGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AATAACAGCA CTATCTCAGA GGAAGATTTT 
CATGTTCTGA CTGAAGCTTT TAATTCAAAC CCAACAAATC TGACAGAGCT GAACTTGAGT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCACAA CTCTGTTTCA AAACCAACAG 
TGTAGATTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT 
TTGACTTCAG CATTTAACTC AAACCCTTCA CACCTGAAAG AGCTCAATCT GAGTGGGAAT 
AAACTAGGAG ACTCAGGAGT AAGAAAAATC TCCAGTCTAC TGAGAAACTC GCAGTGCATG 
CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTTACATCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACCATCAG GAGTGAAGGA GCTCACTGGC TTACTACTCG ATCCACACTG TACACTGACG 
ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GAATTCTGTC AGTTTGTGTC TGGAATTGTG 
GGTAAATACC CATTACTCCT GAAAGAACTG AATCTGAGTG ATCGTGGACT AGGGGACACA 
CGAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT TTAGACTCAA CACACTCATG 
CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TAACTTCAGC ATTTAATTTA 
AATCCTTCAC ACCTGATAGA GCTCAATCTG AGTGGGAATA AACTAGAAGA CTCAGGAGTA 
GAGAAGATCT GTCCTCTGCT GGAAAATATA CAATGCCGAT TAGAGAAACT GAGTCTTTCA 
GACTGCAGTA TCAGGGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGATT TACTGCAGAG TAGAAGCTGT AAATTCAGGT TTCTGAAGAG TTCTGCTGCA 
CAGGAAGCAT GCGAGTATCT TACTAATGTT CTGGGTATAA GTCCATTATT ACTGACAGAA 
CTGGATCTGA GTGAAGTTAA ATTAGGAGAT CTGGACTGGG AGAAACTCTC TGCTCTTCTG 
ATGGACTCTC ATAGCAAAGT GGAGAAAATC AAGCTAAATA ATTGTGAGCT GACAGAGAAA 
AGCTGTTCAG TTCTAGCTGC TGTTCTCTCC TCCAAAACCA TCCTGAAAGA GCTGAACCTG 
AACAACAGTC GTCTGCTGGA CTCAGGAGTC AGAAAGATCT GTGAGGGACT GAAGAATCCT 
GTGTGTGAGC TGAAGATACT CAATCTTTCA GACTGCAGTA TTACAGAAGA AGGTTATGAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGGAG CTCACTGAGT TACTGCAGGA TCCACACTGT 
GCACTGAAGA CACTGAGATT TTTAAGAAGT CCTGCTGCTG ATGAAGGCTG TCAGTTAGTA 
ACTGGAGTTG TGGGTAAAAA CCCGTTACTC CTGAAGGAAC TGAATCTGAG TGATCATGAG 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGCCAGATC 
AACACACTCA ATCTGAGTTA TTGCAGTATT ACAGAGAAAC AGTGTGAGAT CCTGACTTCA 
GCTCTGAAAT CAAACCCATC ACACCTGAGA GAACTGGACC TCAGTGGGAA TGAACTAGGA 
GACTTTCAAA GCCTTGGCTC TCTACTGAAG AACCAACAAT TCAAGCTGGA GAAACTAATT 
CTGTGTGTTT GCAGTATTAA AGAGAAGCAG TGTGAGATCC TGACTTCAGC TCTGAAATCA 
AACCCATCAC ACCTGAGAGA ACTGAACCTC AGTGGGAATG AACTCAAGCA CAGAAGAGTG 
AAGCAATTTT TTGATGTGCT GAAAAAATCA ACCTGTAAAC TGGAGCGACT GAGGTTAAGA 
TACTGTAGTA TACAAGCCGA TGATTGTACT GATCTGGCTT CAGCTCTGAA ATCAAACCCA 
TCCCACCTGA GAGAACTGGA CCTCAGTGGA AACAATCTAG GAGACTCTGG AGTGAAAAAA 
CTCAGTGATT TACTGATAAA CCAACAATTC AAGCTGGAGA AACTACGTCT GAGTTATTGC 
AGTATTACAG AGAAACAGTG TGTGATCCTG ACTTCAGCTC TGAAATCAAA CCCATCACAC 
CTGAGAGAAC TGAACCTCAA TGGGAATAGA CTAGGAGACT CTGGAGCTGA TGGCCTTAAC 
CATCTGCTGA GCAGTGTGGA TTGCAAGCTA AATAAACTAC ATCTGTGTGA TTGCAGTATT 
ACAGAGAAAC AGTTGCTCAT CCCAATCACA GCTACGTGTA TGGGCCTATT ACACCTGAGA 
GAACTGGACC TCAGTAAGAA CCAAATAAAA AACCAAGGAG TGTACATGCT GTGTGATGTG 
CTGAAAAACC CACGCTGTAG ACTCAAGAGG TTAAGACTAA ATGACTGTGG CATTACAGAT 
GTTACACCCT TAACTCAGTC TTTGACCAAG GACAAAGCAT TGAAGTTCTT AAAAGAGCTT 
GAACTGAGTA AAAATCAAAT GGGATTCTTC GAAAAGCAAA AACTCAGTGA CCTGCTGCGA 
GACTCAAACT GTAAACTGAG GCTTGAGGAA GAAGAAGGTT ATTTTAGAGC TGGTGTAAAA 
CGGGTTACTA CATTTTTGAT GCCTCCATGG TCAAAAGTTC AAAAACCTGT GGATCTCACA 
GGAGAGGAAA GTTCATCATC AGATGAGGAC GCCTCTGAAG ATGAGACTGA TCAGGCAGAG 
GAGGCTGATA AGCTCTCAAA TGAGGCGGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_021469421.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325096.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X1, mRNA.

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CloneID	ODa151244
Clone ID Related Accession (Same CDS sequence)	XM_021469421.1
Accession Version	XM_021469421.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein si:ch211-11p18.6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACAACGGGGT GATTGCTGTA AATTAAAAAC CGTCAGGTTT 
TTAAAGAGTT CTGCTGCACA GGAAGCGTGT GACTATCTTA CTAAAGCTGT GGGTAAAAGT 
CCATTATTAC TGACAAAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAA GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTGAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAGATCTGT 
GAGGGGCTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ACCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACTTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGAAGCT CACTGACTTA 
CTGCAGGATC CACACTGTAC CCTGAATACA CTGAGGTTTT TGAGTCCTGC CGCAGAAGAA 
GCCTGTCAGT TTGTGACTGG AATTGTGGGT AAAAACCCGA TACTTCTGAA AGAACTGAAT 
CTGAGTGATC ATGAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AAAAACAGCT TTATCTCAGA GGAAGATTGT 
CGTGTTCTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGAGAGAGCT GAATTTGAAT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCAACA CTCTGTTTCA AAACCAACAG 
TGCAGACTGG AAAAGCTGAA GTTTAGCGGC ATCAGTATCA CAGCAGAAGG TTGTGCTGCT 
TTGACTTCAG CATTTAGCTC AAACCCGTCA CACCTGATAG AGCTAAATCT GAGTGGGAAT 
ACACTAGAAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC ACAGTGCATG 
CTGAAGATAC TCAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGCT TTGATTCCAG AGTGTGCTCT GAAAACATTG 
AGGTTTTTGG GTCCTGCAGC AGAAGAAGCC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCGTTAC TCCTGAAAGA ACTGAATCTG AGTGATCGTA AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC CCAACACACT TCAGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAA TTTAAACCCT 
TCACACCTAA TAGAGCTCAA TCTGAGTGGG AATAAACTAA GAGACTCAGG AGTAGAGGAG 
ATCTGTCCTC TGCTGAAAAA TACACAGTGC AGACTGGAGA GACTAAGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTAAA GGAGCTCACT 
GACTTACTGC AGGATGAGCG ATATAGATTG AAGACCCTCA GGCTTTTGAA AACTACTGCA 
GCACAAGAAG CGTGTGACTA TCTTACTAAA GTTGTGGGTA AAAGTCCATT ATTACTGACA 
GAACTGGATC TGAGTGAAGA TAAATTAGGA GATCTGGATG GAGAGAAACT CTCTGCTCTT 
CTGATGGACT CTCATAGTAA AGTGGAGAAA ATCAAGCTGA ATAATTGTAA GCAGATTGAA 
AAAGGCTGTT CAGTACTAGC TGCCGTTCTC TCCTCAAAAA CAATCCTAAA AGAGATGAAC 
CTGAACAACA GTCGTCTGCT GGACTCAGGA GTCTTAGAGA TCTGTGAGGG ACTGAAGAAC 
CCTGTGTGTG AGCTGAAGAT TCTCAAGCTC AGTAAGTGTG ATTTAACAGA GGAAAGCTGT 
TCAGCTCTGG CTTCAGTTCT CAGATCAGAC TCCAGCAGTC TGAAGGATCT TGACCTGAGC 
AATAATAATC TGCAGGATTC AGGAGTGAAG TTGCTCTCTG ATGGACTAAA GCACAGTAAA 
CTGAAGAGAC TCAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAC TTACTGCAGG ATCCACACTG TACACTAAAG 
ACACTGAGTT GTTTTAGTCC TGCTGCTGAT GAAGGCTGTC AGTTTGTTAC TGGAATTGTG 
GGTAAAAACC CGTTACTACT GAAAGAACTG AATCTGAGTG ATCGTGAGCT AGGAGACACA 
CGGGTGAATC AACTCTCTGC TCTACTGCAA GATAAACACT GTACACTCAA TATACTCACG 
CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAATTTA 
AACCCTTCAC ACCTGATAGA GCTCAATCTG AGTGGAAATA AACTAGGAGA CTCAGGAGTA 
GAGAAGATCT GTCCTCTGCT GAAAAATACC CAGTGCAGAT TGGAGAAATT AAATCTTTCA 
GACTGCAGCA TCACTGGAAA AGGTTATGAA ACATTGATTT CAGCTTTGAA GTCAAACTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGGA AATCAGGAGT GCAGAAGCTC 
ACTGATTTGC TACGAGCTGG AAGCTGTAAA TTAAAAACCA TCAGGACTTT GAAAGGTTCC 
GCTGCACAGG AAGCGTGTGA CTATCTAACT AAAGCTGTGG GTAAAAGTCC ATTATTACTG 
ACAGAACTGG ATCTGAGTGA AGATAAATTA GGAGATCTGG ATGGAGAGAA ACTCTCTGCT 
CTTCTGATGG ACTCTCATAG CAAAGTGGAG AAAATCAGGA TGAATAACTG TAAAAAGCTG 
ACAGAGAAAA GCTGTTTAGT TCTAGCTGCT GTTCTTTCAT CCAAAACCAT CTTGAAAGAG 
ATGAACCTAA ACAACAGTCG TCTGCTGGAC TCAGGAGTCA AAGTGATCTG TGAGGGACTG 
AAGAACTCTA AGCTGGAGAT TTTAAAACTT TCAAATTGTA GTATTACAGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC TGGAGTAAAG GAGCTCACTG AGTTACTGCA GAGTTCGTGG 
ATTCCATGGA AAAAAGTGAA GTGTTCAATG AGGTTTTTGA GTCCTGCTGC TGATGAATTT 
TGTCAGTTTG TGTCTGGAAT TGTGGGTAAA AACCCGTTAC TCCTGAAAGA ACTGAATCTG 
AGTGATCATG TACTAGCAGA CACACAAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA 
CACTGCAAAC TCAACACACT CATTCTTTCA GACTGCAGTA TCAGTGGAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAAGAG CTCACTGAGT TACTGCAGGA TCCACACTGT 
ACACTGAAGA CACTGAGGTT TTTGAGTGAT GATGCAGATG AAGCCTGTGA ACATTTGTAT 
AAAGTTCTGG GTGGAAACCT GTTACTCCTG AGAGAGCTGA ATCTGACTAA ACATGATCTT 
GGACCATCAG GATTAGAGAA ACTTGCTGCA GTACTGAAAG ATAAACACTG TAAACTCAAC 
ACACTGATTC TGAATAACAG CACTATCTCA GAGGAAGATT TTCATGTTCT GACTGAAGCT 
TTTAATTCAA ACCCAACAAA TCTGACAGAG CTGAACTTGA GTGGGACTAA ACTCACAAAC 
TCAGGAATGA AGAGCTTCAC AACTCTGTTT CAAAACCAAC AGTGTAGATT GGAAAAGCTG 
AAGTTCAGCA GCATGTGTAT TACAGCAGAA GGTTGTGCTG CTTTGACTTC AGCATTTAAC 
TCAAACCCTT CACACCTGAA AGAGCTCAAT CTGAGTGGGA ATAAACTAGG AGACTCAGGA 
GTAAGAAAAA TCTCCAGTCT ACTGAGAAAC TCGCAGTGCA TGCTGAAGAT ACTCGGTCTT 
TCAGACTGCA GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTTACATC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACCATC AGGAGTGAAG 
GAGCTCACTG GCTTACTACT CGATCCACAC TGTACACTGA CGACACTGAG GTTTTTGAGT 
CCTGCTGCTG ATGAATTCTG TCAGTTTGTG TCTGGAATTG TGGGTAAATA CCCATTACTC 
CTGAAAGAAC TGAATCTGAG TGATCGTGGA CTAGGGGACA CACGAGTGAA TCAACTCTCT 
GCTCTACTGC AGGATAAACA CTTTAGACTC AACACACTCA TGCTGAATAA TAACAGGATT 
ACAGCAGAAG GTTGTGCTGC TTTAACTTCA GCATTTAATT TAAATCCTTC ACACCTGATA 
GAGCTCAATC TGAGTGGGAA TAAACTAGAA GACTCAGGAG TAGAGAAGAT CTGTCCTCTG 
CTGGAAAATA TACAATGCCG ATTAGAGAAA CTGAGTCTTT CAGACTGCAG TATCAGGGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA TTTACTGCAG 
AGTAGAAGCT GTAAATTCAG GTTTCTGAAG AGTTCTGCTG CACAGGAAGC ATGCGAGTAT 
CTTACTAATG TTCTGGGTAT AAGTCCATTA TTACTGACAG AACTGGATCT GAGTGAAGTT 
AAATTAGGAG ATCTGGACTG GGAGAAACTC TCTGCTCTTC TGATGGACTC TCATAGCAAA 
GTGGAGAAAA TCAAGCTAAA TAATTGTGAG CTGACAGAGA AAAGCTGTTC AGTTCTAGCT 
GCTGTTCTCT CCTCCAAAAC CATCCTGAAA GAGCTGAACC TGAACAACAG TCGTCTGCTG 
GACTCAGGAG TCAGAAAGAT CTGTGAGGGA CTGAAGAATC CTGTGTGTGA GCTGAAGATA 
CTCAATCTTT CAGACTGCAG TATTACAGAA GAAGGTTATG AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA GTTACTGCAG GATCCACACT GTGCACTGAA GACACTGAGA 
TTTTTAAGAA GTCCTGCTGC TGATGAAGGC TGTCAGTTAG TAACTGGAGT TGTGGGTAAA 
AACCCGTTAC TCCTGAAGGA ACTGAATCTG AGTGATCATG AGCTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGCCAGA TCAACACACT CAATCTGAGT 
TATTGCAGTA TTACAGAGAA ACAGTGTGAG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGGA CCTCAGTGGG AATGAACTAG GAGACTTTCA AAGCCTTGGC 
TCTCTACTGA AGAACCAACA ATTCAAGCTG GAGAAACTAA TTCTGTGTGT TTGCAGTATT 
AAAGAGAAGC AGTGTGAGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA 
GAACTGAACC TCAGTGGGAA TGAACTCAAG CACAGAAGAG TGAAGCAATT TTTTGATGTG 
CTGAAAAAAT CAACCTGTAA ACTGGAGCGA CTGAGGTTAA GATACTGTAG TATACAAGCC 
GATGATTGTA CTGATCTGGC TTCAGCTCTG AAATCAAACC CATCCCACCT GAGAGAACTG 
GACCTCAGTG GAAACAATCT AGGAGACTCT GGAGTGAAAA AACTCAGTGA TTTACTGATA 
AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTTATT GCAGTATTAC AGAGAAACAG 
TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC 
AATGGGAATA GACTAGGAGA CTCTGGAGCT GATGGCCTTA ACCATCTGCT GAGCAGTGTG 
GATTGCAAGC TAAATAAACT ACATCTGTGT GATTGCAGTA TTACAGAGAA ACAGTTGCTC 
ATCCCAATCA CAGCTACGTG TATGGGCCTA TTACACCTGA GAGAACTGGA CCTCAGTAAG 
AACCAAATAA AAAACCAAGG AGTGTACATG CTGTGTGATG TGCTGAAAAA CCCACGCTGT 
AGACTCAAGA GGTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACC CTTAACTCAG 
TCTTTGACCA AGGACAAAGC ATTGAAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA 
ATGGGATTCT TCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG 
AGGCTTGAGG AAGAAGAAGG TTATTTTAGA GCTGGTGTAA AACGGGTTAC TACATTTTTG 
ATGCCTCCAT GGTCAAAAGT TCAAAAACCT GTGGATCTCA CAGGAGAGGA AAGTTCATCA 
TCAGATGAGG ACGCCTCTGA AGATGAGACT GATCAGGCAG AGGAGGCTGA TAAGCTCTCA 
AATGAGGCGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325096.1
CDS	66..8960
Translation	MSNNNLQDSGVKLLCTALKNVKCELEVLRISYCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGILGKNPLLLKELNLSDCELGDTGVNQLSALLQDKHCKPKTLMLRGCGLKEETCSALATVLGSDTSLKELDMSNNNLQDLGVKKLQSGLENTNCTLEKLSLSDCNISEEGYKALASALRSNPSHLIELNLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAAEEFCQFVTGIVGKNPLLLKELYLSDHELGDTRVNQLSALLQDKHCTVSTLILHNCGLTKESCSALASALSSDTSLKELDLCNNNLQDSGVKKLQSELQNTNWRLKKLRLSKCDLTEESCSALASVLTSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSKLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELIELLQDPLCTLKTLRFLSPAADEACQFVTGIVGKNLLLLKELNLSDRQLGDTRVNQLSALLQDKHCTLNTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLKFSGISITAEGCAALTSAFSSNPSHLIELNLSGNTLEDSGVTEISSLLANSQCMLKILSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACEHLYKVLGGNLLLLRELNLTKHDLGPSGLEKLAAVLKDKHCKLNTLILNNSTISEEDFHVLTEAFNSNPTNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_021469421.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469421.1

ORF Insert Sequence:

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACAACGGGGT GATTGCTGTA AATTAAAAAC CGTCAGGTTT 
TTAAAGAGTT CTGCTGCACA GGAAGCGTGT GACTATCTTA CTAAAGCTGT GGGTAAAAGT 
CCATTATTAC TGACAAAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAA GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTGAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAGATCTGT 
GAGGGGCTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ACCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACTTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGAAGCT CACTGACTTA 
CTGCAGGATC CACACTGTAC CCTGAATACA CTGAGGTTTT TGAGTCCTGC CGCAGAAGAA 
GCCTGTCAGT TTGTGACTGG AATTGTGGGT AAAAACCCGA TACTTCTGAA AGAACTGAAT 
CTGAGTGATC ATGAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AAAAACAGCT TTATCTCAGA GGAAGATTGT 
CGTGTTCTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGAGAGAGCT GAATTTGAAT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCAACA CTCTGTTTCA AAACCAACAG 
TGCAGACTGG AAAAGCTGAA GTTTAGCGGC ATCAGTATCA CAGCAGAAGG TTGTGCTGCT 
TTGACTTCAG CATTTAGCTC AAACCCGTCA CACCTGATAG AGCTAAATCT GAGTGGGAAT 
ACACTAGAAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC ACAGTGCATG 
CTGAAGATAC TCAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGCT TTGATTCCAG AGTGTGCTCT GAAAACATTG 
AGGTTTTTGG GTCCTGCAGC AGAAGAAGCC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCGTTAC TCCTGAAAGA ACTGAATCTG AGTGATCGTA AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC CCAACACACT TCAGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAA TTTAAACCCT 
TCACACCTAA TAGAGCTCAA TCTGAGTGGG AATAAACTAA GAGACTCAGG AGTAGAGGAG 
ATCTGTCCTC TGCTGAAAAA TACACAGTGC AGACTGGAGA GACTAAGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTAAA GGAGCTCACT 
GACTTACTGC AGGATGAGCG ATATAGATTG AAGACCCTCA GGCTTTTGAA AACTACTGCA 
GCACAAGAAG CGTGTGACTA TCTTACTAAA GTTGTGGGTA AAAGTCCATT ATTACTGACA 
GAACTGGATC TGAGTGAAGA TAAATTAGGA GATCTGGATG GAGAGAAACT CTCTGCTCTT 
CTGATGGACT CTCATAGTAA AGTGGAGAAA ATCAAGCTGA ATAATTGTAA GCAGATTGAA 
AAAGGCTGTT CAGTACTAGC TGCCGTTCTC TCCTCAAAAA CAATCCTAAA AGAGATGAAC 
CTGAACAACA GTCGTCTGCT GGACTCAGGA GTCTTAGAGA TCTGTGAGGG ACTGAAGAAC 
CCTGTGTGTG AGCTGAAGAT TCTCAAGCTC AGTAAGTGTG ATTTAACAGA GGAAAGCTGT 
TCAGCTCTGG CTTCAGTTCT CAGATCAGAC TCCAGCAGTC TGAAGGATCT TGACCTGAGC 
AATAATAATC TGCAGGATTC AGGAGTGAAG TTGCTCTCTG ATGGACTAAA GCACAGTAAA 
CTGAAGAGAC TCAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAC TTACTGCAGG ATCCACACTG TACACTAAAG 
ACACTGAGTT GTTTTAGTCC TGCTGCTGAT GAAGGCTGTC AGTTTGTTAC TGGAATTGTG 
GGTAAAAACC CGTTACTACT GAAAGAACTG AATCTGAGTG ATCGTGAGCT AGGAGACACA 
CGGGTGAATC AACTCTCTGC TCTACTGCAA GATAAACACT GTACACTCAA TATACTCACG 
CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAATTTA 
AACCCTTCAC ACCTGATAGA GCTCAATCTG AGTGGAAATA AACTAGGAGA CTCAGGAGTA 
GAGAAGATCT GTCCTCTGCT GAAAAATACC CAGTGCAGAT TGGAGAAATT AAATCTTTCA 
GACTGCAGCA TCACTGGAAA AGGTTATGAA ACATTGATTT CAGCTTTGAA GTCAAACTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGGA AATCAGGAGT GCAGAAGCTC 
ACTGATTTGC TACGAGCTGG AAGCTGTAAA TTAAAAACCA TCAGGACTTT GAAAGGTTCC 
GCTGCACAGG AAGCGTGTGA CTATCTAACT AAAGCTGTGG GTAAAAGTCC ATTATTACTG 
ACAGAACTGG ATCTGAGTGA AGATAAATTA GGAGATCTGG ATGGAGAGAA ACTCTCTGCT 
CTTCTGATGG ACTCTCATAG CAAAGTGGAG AAAATCAGGA TGAATAACTG TAAAAAGCTG 
ACAGAGAAAA GCTGTTTAGT TCTAGCTGCT GTTCTTTCAT CCAAAACCAT CTTGAAAGAG 
ATGAACCTAA ACAACAGTCG TCTGCTGGAC TCAGGAGTCA AAGTGATCTG TGAGGGACTG 
AAGAACTCTA AGCTGGAGAT TTTAAAACTT TCAAATTGTA GTATTACAGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC TGGAGTAAAG GAGCTCACTG AGTTACTGCA GAGTTCGTGG 
ATTCCATGGA AAAAAGTGAA GTGTTCAATG AGGTTTTTGA GTCCTGCTGC TGATGAATTT 
TGTCAGTTTG TGTCTGGAAT TGTGGGTAAA AACCCGTTAC TCCTGAAAGA ACTGAATCTG 
AGTGATCATG TACTAGCAGA CACACAAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA 
CACTGCAAAC TCAACACACT CATTCTTTCA GACTGCAGTA TCAGTGGAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAAGAG CTCACTGAGT TACTGCAGGA TCCACACTGT 
ACACTGAAGA CACTGAGGTT TTTGAGTGAT GATGCAGATG AAGCCTGTGA ACATTTGTAT 
AAAGTTCTGG GTGGAAACCT GTTACTCCTG AGAGAGCTGA ATCTGACTAA ACATGATCTT 
GGACCATCAG GATTAGAGAA ACTTGCTGCA GTACTGAAAG ATAAACACTG TAAACTCAAC 
ACACTGATTC TGAATAACAG CACTATCTCA GAGGAAGATT TTCATGTTCT GACTGAAGCT 
TTTAATTCAA ACCCAACAAA TCTGACAGAG CTGAACTTGA GTGGGACTAA ACTCACAAAC 
TCAGGAATGA AGAGCTTCAC AACTCTGTTT CAAAACCAAC AGTGTAGATT GGAAAAGCTG 
AAGTTCAGCA GCATGTGTAT TACAGCAGAA GGTTGTGCTG CTTTGACTTC AGCATTTAAC 
TCAAACCCTT CACACCTGAA AGAGCTCAAT CTGAGTGGGA ATAAACTAGG AGACTCAGGA 
GTAAGAAAAA TCTCCAGTCT ACTGAGAAAC TCGCAGTGCA TGCTGAAGAT ACTCGGTCTT 
TCAGACTGCA GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTTACATC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACCATC AGGAGTGAAG 
GAGCTCACTG GCTTACTACT CGATCCACAC TGTACACTGA CGACACTGAG GTTTTTGAGT 
CCTGCTGCTG ATGAATTCTG TCAGTTTGTG TCTGGAATTG TGGGTAAATA CCCATTACTC 
CTGAAAGAAC TGAATCTGAG TGATCGTGGA CTAGGGGACA CACGAGTGAA TCAACTCTCT 
GCTCTACTGC AGGATAAACA CTTTAGACTC AACACACTCA TGCTGAATAA TAACAGGATT 
ACAGCAGAAG GTTGTGCTGC TTTAACTTCA GCATTTAATT TAAATCCTTC ACACCTGATA 
GAGCTCAATC TGAGTGGGAA TAAACTAGAA GACTCAGGAG TAGAGAAGAT CTGTCCTCTG 
CTGGAAAATA TACAATGCCG ATTAGAGAAA CTGAGTCTTT CAGACTGCAG TATCAGGGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA TTTACTGCAG 
AGTAGAAGCT GTAAATTCAG GTTTCTGAAG AGTTCTGCTG CACAGGAAGC ATGCGAGTAT 
CTTACTAATG TTCTGGGTAT AAGTCCATTA TTACTGACAG AACTGGATCT GAGTGAAGTT 
AAATTAGGAG ATCTGGACTG GGAGAAACTC TCTGCTCTTC TGATGGACTC TCATAGCAAA 
GTGGAGAAAA TCAAGCTAAA TAATTGTGAG CTGACAGAGA AAAGCTGTTC AGTTCTAGCT 
GCTGTTCTCT CCTCCAAAAC CATCCTGAAA GAGCTGAACC TGAACAACAG TCGTCTGCTG 
GACTCAGGAG TCAGAAAGAT CTGTGAGGGA CTGAAGAATC CTGTGTGTGA GCTGAAGATA 
CTCAATCTTT CAGACTGCAG TATTACAGAA GAAGGTTATG AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA GTTACTGCAG GATCCACACT GTGCACTGAA GACACTGAGA 
TTTTTAAGAA GTCCTGCTGC TGATGAAGGC TGTCAGTTAG TAACTGGAGT TGTGGGTAAA 
AACCCGTTAC TCCTGAAGGA ACTGAATCTG AGTGATCATG AGCTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGCCAGA TCAACACACT CAATCTGAGT 
TATTGCAGTA TTACAGAGAA ACAGTGTGAG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGGA CCTCAGTGGG AATGAACTAG GAGACTTTCA AAGCCTTGGC 
TCTCTACTGA AGAACCAACA ATTCAAGCTG GAGAAACTAA TTCTGTGTGT TTGCAGTATT 
AAAGAGAAGC AGTGTGAGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA 
GAACTGAACC TCAGTGGGAA TGAACTCAAG CACAGAAGAG TGAAGCAATT TTTTGATGTG 
CTGAAAAAAT CAACCTGTAA ACTGGAGCGA CTGAGGTTAA GATACTGTAG TATACAAGCC 
GATGATTGTA CTGATCTGGC TTCAGCTCTG AAATCAAACC CATCCCACCT GAGAGAACTG 
GACCTCAGTG GAAACAATCT AGGAGACTCT GGAGTGAAAA AACTCAGTGA TTTACTGATA 
AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTTATT GCAGTATTAC AGAGAAACAG 
TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC 
AATGGGAATA GACTAGGAGA CTCTGGAGCT GATGGCCTTA ACCATCTGCT GAGCAGTGTG 
GATTGCAAGC TAAATAAACT ACATCTGTGT GATTGCAGTA TTACAGAGAA ACAGTTGCTC 
ATCCCAATCA CAGCTACGTG TATGGGCCTA TTACACCTGA GAGAACTGGA CCTCAGTAAG 
AACCAAATAA AAAACCAAGG AGTGTACATG CTGTGTGATG TGCTGAAAAA CCCACGCTGT 
AGACTCAAGA GGTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACC CTTAACTCAG 
TCTTTGACCA AGGACAAAGC ATTGAAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA 
ATGGGATTCT TCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG 
AGGCTTGAGG AAGAAGAAGG TTATTTTAGA GCTGGTGTAA AACGGGTTAC TACATTTTTG 
ATGCCTCCAT GGTCAAAAGT TCAAAAACCT GTGGATCTCA CAGGAGAGGA AAGTTCATCA 
TCAGATGAGG ACGCCTCTGA AGATGAGACT GATCAGGCAG AGGAGGCTGA TAAGCTCTCA 
AATGAGGCGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_021469422.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325097.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X2, mRNA.

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CloneID	ODa151245
Clone ID Related Accession (Same CDS sequence)	XM_021469422.1
Accession Version	XM_021469422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein si:ch211-11p18.6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAATGGTG CTGCAGATGA AGCCTGTAAA CATCTGAGTA AAGTTCTGGG TGGAAACCTG 
TTACTCCTGA AAGAGCTGAA TCTGACTGAA CATGATCACG GATACTTAGG ATTAAAGAAA 
CTTGCTGCTG TACTGAAAGA TAAACACTGT AAACTCAACA CACTGATTCT GAAAAACAGC 
TTTATCTCAG AGGAAGATTG TCGTGTTCTG GCTGAAGCTT TTAATTCAAA CCCATCAAAT 
CTGAGAGAGC TGAATTTGAA TGGGACTAAA CTCACAAACT CAGGAATGAA GAGCTTCAAC 
ACTCTGTTTC AAAACCAACA GTGCAGACTG GAAAAGCTGA AGTTTAGCGG CATCAGTATC 
ACAGCAGAAG GTTGTGCTGC TTTGACTTCA GCATTTAGCT CAAACCCGTC ACACCTGATA 
GAGCTAAATC TGAGTGGGAA TACACTAGAA GACTCAGGAG TAACAGAAAT CTCCAGTCTA 
CTGGCAAACT CACAGTGCAT GCTGAAGATA CTCAGTCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGC TTTGATTCCA 
GAGTGTGCTC TGAAAACATT GAGGTTTTTG GGTCCTGCAG CAGAAGAAGC CTGTCAGTTT 
GTGACTGGAA TTGTGGGTAA AAACCCGTTA CTCCTGAAAG AACTGAATCT GAGTGATCGT 
AAACTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGTAAA 
CCCAACACAC TTCAGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTAACT 
TCAGCATTTA ATTTAAACCC TTCACACCTA ATAGAGCTCA ATCTGAGTGG GAATAAACTA 
AGAGACTCAG GAGTAGAGGA GATCTGTCCT CTGCTGAAAA ATACACAGTG CAGACTGGAG 
AGACTAAGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTAA AGGAGCTCAC TGACTTACTG CAGGATGAGC GATATAGATT GAAGACCCTC 
AGGCTTTTGA AAACTACTGC AGCACAAGAA GCGTGTGACT ATCTTACTAA AGTTGTGGGT 
AAAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG ATAAATTAGG AGATCTGGAT 
GGAGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGTA AAGTGGAGAA AATCAAGCTG 
AATAATTGTA AGCAGATTGA AAAAGGCTGT TCAGTACTAG CTGCCGTTCT CTCCTCAAAA 
ACAATCCTAA AAGAGATGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCTTAGAG 
ATCTGTGAGG GACTGAAGAA CCCTGTGTGT GAGCTGAAGA TTCTCAAGCT CAGTAAGTGT 
GATTTAACAG AGGAAAGCTG TTCAGCTCTG GCTTCAGTTC TCAGATCAGA CTCCAGCAGT 
CTGAAGGATC TTGACCTGAG CAATAATAAT CTGCAGGATT CAGGAGTGAA GTTGCTCTCT 
GATGGACTAA AGCACAGTAA ACTGAAGAGA CTCAGTCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA CTTACTGCAG 
GATCCACACT GTACACTAAA GACACTGAGT TGTTTTAGTC CTGCTGCTGA TGAAGGCTGT 
CAGTTTGTTA CTGGAATTGT GGGTAAAAAC CCGTTACTAC TGAAAGAACT GAATCTGAGT 
GATCGTGAGC TAGGAGACAC ACGGGTGAAT CAACTCTCTG CTCTACTGCA AGATAAACAC 
TGTACACTCA ATATACTCAC GCTGAATAAT AACAGGATTA CAGCAGAAGG TTGTGCTGCT 
TTGACTTCAG CATTTAATTT AAACCCTTCA CACCTGATAG AGCTCAATCT GAGTGGAAAT 
AAACTAGGAG ACTCAGGAGT AGAGAAGATC TGTCCTCTGC TGAAAAATAC CCAGTGCAGA 
TTGGAGAAAT TAAATCTTTC AGACTGCAGC ATCACTGGAA AAGGTTATGA AACATTGATT 
TCAGCTTTGA AGTCAAACTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGG 
AAATCAGGAG TGCAGAAGCT CACTGATTTG CTACGAGCTG GAAGCTGTAA ATTAAAAACC 
ATCAGGACTT TGAAAGGTTC CGCTGCACAG GAAGCGTGTG ACTATCTAAC TAAAGCTGTG 
GGTAAAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG 
GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGGA GAAAATCAGG 
ATGAATAACT GTAAAAAGCT GACAGAGAAA AGCTGTTTAG TTCTAGCTGC TGTTCTTTCA 
TCCAAAACCA TCTTGAAAGA GATGAACCTA AACAACAGTC GTCTGCTGGA CTCAGGAGTC 
AAAGTGATCT GTGAGGGACT GAAGAACTCT AAGCTGGAGA TTTTAAAACT TTCAAATTGT 
AGTATTACAG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CTGGAGTAAA GGAGCTCACT 
GAGTTACTGC AGAGTTCGTG GATTCCATGG AAAAAAGTGA AGTGTTCAAT GAGGTTTTTG 
AGTCCTGCTG CTGATGAATT TTGTCAGTTT GTGTCTGGAA TTGTGGGTAA AAACCCGTTA 
CTCCTGAAAG AACTGAATCT GAGTGATCAT GTACTAGCAG ACACACAAGT GAATCAACTC 
TCTGCTCTAC TGCAGGATAA ACACTGCAAA CTCAACACAC TCATTCTTTC AGACTGCAGT 
ATCAGTGGAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAAGA GCTCACTGAG 
TTACTGCAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTGA TGATGCAGAT 
GAAGCCTGTG AACATTTGTA TAAAGTTCTG GGTGGAAACC TGTTACTCCT GAGAGAGCTG 
AATCTGACTA AACATGATCT TGGACCATCA GGATTAGAGA AACTTGCTGC AGTACTGAAA 
GATAAACACT GTAAACTCAA CACACTGATT CTGAATAACA GCACTATCTC AGAGGAAGAT 
TTTCATGTTC TGACTGAAGC TTTTAATTCA AACCCAACAA ATCTGACAGA GCTGAACTTG 
AGTGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA CAACTCTGTT TCAAAACCAA 
CAGTGTAGAT TGGAAAAGCT GAAGTTCAGC AGCATGTGTA TTACAGCAGA AGGTTGTGCT 
GCTTTGACTT CAGCATTTAA CTCAAACCCT TCACACCTGA AAGAGCTCAA TCTGAGTGGG 
AATAAACTAG GAGACTCAGG AGTAAGAAAA ATCTCCAGTC TACTGAGAAA CTCGCAGTGC 
ATGCTGAAGA TACTCGGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTTACAT CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACCAT CAGGAGTGAA GGAGCTCACT GGCTTACTAC TCGATCCACA CTGTACACTG 
ACGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAATTCT GTCAGTTTGT GTCTGGAATT 
GTGGGTAAAT ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGG ACTAGGGGAC 
ACACGAGTGA ATCAACTCTC TGCTCTACTG CAGGATAAAC ACTTTAGACT CAACACACTC 
ATGCTGAATA ATAACAGGAT TACAGCAGAA GGTTGTGCTG CTTTAACTTC AGCATTTAAT 
TTAAATCCTT CACACCTGAT AGAGCTCAAT CTGAGTGGGA ATAAACTAGA AGACTCAGGA 
GTAGAGAAGA TCTGTCCTCT GCTGGAAAAT ATACAATGCC GATTAGAGAA ACTGAGTCTT 
TCAGACTGCA GTATCAGGGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG ATTTACTGCA GAGTAGAAGC TGTAAATTCA GGTTTCTGAA GAGTTCTGCT 
GCACAGGAAG CATGCGAGTA TCTTACTAAT GTTCTGGGTA TAAGTCCATT ATTACTGACA 
GAACTGGATC TGAGTGAAGT TAAATTAGGA GATCTGGACT GGGAGAAACT CTCTGCTCTT 
CTGATGGACT CTCATAGCAA AGTGGAGAAA ATCAAGCTAA ATAATTGTGA GCTGACAGAG 
AAAAGCTGTT CAGTTCTAGC TGCTGTTCTC TCCTCCAAAA CCATCCTGAA AGAGCTGAAC 
CTGAACAACA GTCGTCTGCT GGACTCAGGA GTCAGAAAGA TCTGTGAGGG ACTGAAGAAT 
CCTGTGTGTG AGCTGAAGAT ACTCAATCTT TCAGACTGCA GTATTACAGA AGAAGGTTAT 
GAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTGCACTGA AGACACTGAG ATTTTTAAGA AGTCCTGCTG CTGATGAAGG CTGTCAGTTA 
GTAACTGGAG TTGTGGGTAA AAACCCGTTA CTCCTGAAGG AACTGAATCT GAGTGATCAT 
GAGCTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGCCAG 
ATCAACACAC TCAATCTGAG TTATTGCAGT ATTACAGAGA AACAGTGTGA GATCCTGACT 
TCAGCTCTGA AATCAAACCC ATCACACCTG AGAGAACTGG ACCTCAGTGG GAATGAACTA 
GGAGACTTTC AAAGCCTTGG CTCTCTACTG AAGAACCAAC AATTCAAGCT GGAGAAACTA 
ATTCTGTGTG TTTGCAGTAT TAAAGAGAAG CAGTGTGAGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGGA ATGAACTCAA GCACAGAAGA 
GTGAAGCAAT TTTTTGATGT GCTGAAAAAA TCAACCTGTA AACTGGAGCG ACTGAGGTTA 
AGATACTGTA GTATACAAGC CGATGATTGT ACTGATCTGG CTTCAGCTCT GAAATCAAAC 
CCATCCCACC TGAGAGAACT GGACCTCAGT GGAAACAATC TAGGAGACTC TGGAGTGAAA 
AAACTCAGTG ATTTACTGAT AAACCAACAA TTCAAGCTGG AGAAACTACG TCTGAGTTAT 
TGCAGTATTA CAGAGAAACA GTGTGTGATC CTGACTTCAG CTCTGAAATC AAACCCATCA 
CACCTGAGAG AACTGAACCT CAATGGGAAT AGACTAGGAG ACTCTGGAGC TGATGGCCTT 
AACCATCTGC TGAGCAGTGT GGATTGCAAG CTAAATAAAC TACATCTGTG TGATTGCAGT 
ATTACAGAGA AACAGTTGCT CATCCCAATC ACAGCTACGT GTATGGGCCT ATTACACCTG 
AGAGAACTGG ACCTCAGTAA GAACCAAATA AAAAACCAAG GAGTGTACAT GCTGTGTGAT 
GTGCTGAAAA ACCCACGCTG TAGACTCAAG AGGTTAAGAC TAAATGACTG TGGCATTACA 
GATGTTACAC CCTTAACTCA GTCTTTGACC AAGGACAAAG CATTGAAGTT CTTAAAAGAG 
CTTGAACTGA GTAAAAATCA AATGGGATTC TTCGAAAAGC AAAAACTCAG TGACCTGCTG 
CGAGACTCAA ACTGTAAACT GAGGCTTGAG GAAGAAGAAG GTTATTTTAG AGCTGGTGTA 
AAACGGGTTA CTACATTTTT GATGCCTCCA TGGTCAAAAG TTCAAAAACC TGTGGATCTC 
ACAGGAGAGG AAAGTTCATC ATCAGATGAG GACGCCTCTG AAGATGAGAC TGATCAGGCA 
GAGGAGGCTG ATAAGCTCTC AAATGAGGCG GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325097.1
CDS	66..8441
Translation	MSNNNLQDSGVKLLCTALKNVKCELEVLRISYCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGILGKNPLLLKELNLSDCELGDTGVNQLSALLQDKHCKPKTLMLRGCGLKEETCSALATVLGSDTSLKELDMSNNNLQDLGVKKLQSGLENTNCTLEKLSLSDCNISEEGYKALASALRSNPSHLIELNLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAAEEFCQFVTGIVGKNPLLLKELYLSDHELGDTRVNQLSALLQDKHCTVSTLILHNCGLTKESCSALASALSSDTSLKELDLCNNNLQDSGVKKLQSELQNTNWRLKKLRLSKCDLTEESCSALASVLTSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSKLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELIELLQDPLCTLKTLRFLSPAADEACQFVTGIVGKNLLLLKELNLSDRQLGDTRVNQLSALLQDKHCTLNTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLNGAADEACKHLSKVLGGNLLLLKELNLTEHDHGYLGLKKLAAVLKDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLKFSGISITAEGCAALTSAFSSNPSHLIELNLSGNTLEDSGVTEISSLLANSQCMLKILSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACEHLYKVLGGNLLLLRELNLTKHDLGPSGLEKLAAVLKDKHCKLNTLILNNSTISEEDFHVLTEAFNSNPTNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_021469422.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469422.1

ORF Insert Sequence:

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAATGGTG CTGCAGATGA AGCCTGTAAA CATCTGAGTA AAGTTCTGGG TGGAAACCTG 
TTACTCCTGA AAGAGCTGAA TCTGACTGAA CATGATCACG GATACTTAGG ATTAAAGAAA 
CTTGCTGCTG TACTGAAAGA TAAACACTGT AAACTCAACA CACTGATTCT GAAAAACAGC 
TTTATCTCAG AGGAAGATTG TCGTGTTCTG GCTGAAGCTT TTAATTCAAA CCCATCAAAT 
CTGAGAGAGC TGAATTTGAA TGGGACTAAA CTCACAAACT CAGGAATGAA GAGCTTCAAC 
ACTCTGTTTC AAAACCAACA GTGCAGACTG GAAAAGCTGA AGTTTAGCGG CATCAGTATC 
ACAGCAGAAG GTTGTGCTGC TTTGACTTCA GCATTTAGCT CAAACCCGTC ACACCTGATA 
GAGCTAAATC TGAGTGGGAA TACACTAGAA GACTCAGGAG TAACAGAAAT CTCCAGTCTA 
CTGGCAAACT CACAGTGCAT GCTGAAGATA CTCAGTCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGC TTTGATTCCA 
GAGTGTGCTC TGAAAACATT GAGGTTTTTG GGTCCTGCAG CAGAAGAAGC CTGTCAGTTT 
GTGACTGGAA TTGTGGGTAA AAACCCGTTA CTCCTGAAAG AACTGAATCT GAGTGATCGT 
AAACTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGTAAA 
CCCAACACAC TTCAGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTAACT 
TCAGCATTTA ATTTAAACCC TTCACACCTA ATAGAGCTCA ATCTGAGTGG GAATAAACTA 
AGAGACTCAG GAGTAGAGGA GATCTGTCCT CTGCTGAAAA ATACACAGTG CAGACTGGAG 
AGACTAAGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTAA AGGAGCTCAC TGACTTACTG CAGGATGAGC GATATAGATT GAAGACCCTC 
AGGCTTTTGA AAACTACTGC AGCACAAGAA GCGTGTGACT ATCTTACTAA AGTTGTGGGT 
AAAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG ATAAATTAGG AGATCTGGAT 
GGAGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGTA AAGTGGAGAA AATCAAGCTG 
AATAATTGTA AGCAGATTGA AAAAGGCTGT TCAGTACTAG CTGCCGTTCT CTCCTCAAAA 
ACAATCCTAA AAGAGATGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCTTAGAG 
ATCTGTGAGG GACTGAAGAA CCCTGTGTGT GAGCTGAAGA TTCTCAAGCT CAGTAAGTGT 
GATTTAACAG AGGAAAGCTG TTCAGCTCTG GCTTCAGTTC TCAGATCAGA CTCCAGCAGT 
CTGAAGGATC TTGACCTGAG CAATAATAAT CTGCAGGATT CAGGAGTGAA GTTGCTCTCT 
GATGGACTAA AGCACAGTAA ACTGAAGAGA CTCAGTCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA CTTACTGCAG 
GATCCACACT GTACACTAAA GACACTGAGT TGTTTTAGTC CTGCTGCTGA TGAAGGCTGT 
CAGTTTGTTA CTGGAATTGT GGGTAAAAAC CCGTTACTAC TGAAAGAACT GAATCTGAGT 
GATCGTGAGC TAGGAGACAC ACGGGTGAAT CAACTCTCTG CTCTACTGCA AGATAAACAC 
TGTACACTCA ATATACTCAC GCTGAATAAT AACAGGATTA CAGCAGAAGG TTGTGCTGCT 
TTGACTTCAG CATTTAATTT AAACCCTTCA CACCTGATAG AGCTCAATCT GAGTGGAAAT 
AAACTAGGAG ACTCAGGAGT AGAGAAGATC TGTCCTCTGC TGAAAAATAC CCAGTGCAGA 
TTGGAGAAAT TAAATCTTTC AGACTGCAGC ATCACTGGAA AAGGTTATGA AACATTGATT 
TCAGCTTTGA AGTCAAACTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGG 
AAATCAGGAG TGCAGAAGCT CACTGATTTG CTACGAGCTG GAAGCTGTAA ATTAAAAACC 
ATCAGGACTT TGAAAGGTTC CGCTGCACAG GAAGCGTGTG ACTATCTAAC TAAAGCTGTG 
GGTAAAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG 
GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGGA GAAAATCAGG 
ATGAATAACT GTAAAAAGCT GACAGAGAAA AGCTGTTTAG TTCTAGCTGC TGTTCTTTCA 
TCCAAAACCA TCTTGAAAGA GATGAACCTA AACAACAGTC GTCTGCTGGA CTCAGGAGTC 
AAAGTGATCT GTGAGGGACT GAAGAACTCT AAGCTGGAGA TTTTAAAACT TTCAAATTGT 
AGTATTACAG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CTGGAGTAAA GGAGCTCACT 
GAGTTACTGC AGAGTTCGTG GATTCCATGG AAAAAAGTGA AGTGTTCAAT GAGGTTTTTG 
AGTCCTGCTG CTGATGAATT TTGTCAGTTT GTGTCTGGAA TTGTGGGTAA AAACCCGTTA 
CTCCTGAAAG AACTGAATCT GAGTGATCAT GTACTAGCAG ACACACAAGT GAATCAACTC 
TCTGCTCTAC TGCAGGATAA ACACTGCAAA CTCAACACAC TCATTCTTTC AGACTGCAGT 
ATCAGTGGAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAAGA GCTCACTGAG 
TTACTGCAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTGA TGATGCAGAT 
GAAGCCTGTG AACATTTGTA TAAAGTTCTG GGTGGAAACC TGTTACTCCT GAGAGAGCTG 
AATCTGACTA AACATGATCT TGGACCATCA GGATTAGAGA AACTTGCTGC AGTACTGAAA 
GATAAACACT GTAAACTCAA CACACTGATT CTGAATAACA GCACTATCTC AGAGGAAGAT 
TTTCATGTTC TGACTGAAGC TTTTAATTCA AACCCAACAA ATCTGACAGA GCTGAACTTG 
AGTGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA CAACTCTGTT TCAAAACCAA 
CAGTGTAGAT TGGAAAAGCT GAAGTTCAGC AGCATGTGTA TTACAGCAGA AGGTTGTGCT 
GCTTTGACTT CAGCATTTAA CTCAAACCCT TCACACCTGA AAGAGCTCAA TCTGAGTGGG 
AATAAACTAG GAGACTCAGG AGTAAGAAAA ATCTCCAGTC TACTGAGAAA CTCGCAGTGC 
ATGCTGAAGA TACTCGGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTTACAT CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACCAT CAGGAGTGAA GGAGCTCACT GGCTTACTAC TCGATCCACA CTGTACACTG 
ACGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAATTCT GTCAGTTTGT GTCTGGAATT 
GTGGGTAAAT ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGG ACTAGGGGAC 
ACACGAGTGA ATCAACTCTC TGCTCTACTG CAGGATAAAC ACTTTAGACT CAACACACTC 
ATGCTGAATA ATAACAGGAT TACAGCAGAA GGTTGTGCTG CTTTAACTTC AGCATTTAAT 
TTAAATCCTT CACACCTGAT AGAGCTCAAT CTGAGTGGGA ATAAACTAGA AGACTCAGGA 
GTAGAGAAGA TCTGTCCTCT GCTGGAAAAT ATACAATGCC GATTAGAGAA ACTGAGTCTT 
TCAGACTGCA GTATCAGGGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG ATTTACTGCA GAGTAGAAGC TGTAAATTCA GGTTTCTGAA GAGTTCTGCT 
GCACAGGAAG CATGCGAGTA TCTTACTAAT GTTCTGGGTA TAAGTCCATT ATTACTGACA 
GAACTGGATC TGAGTGAAGT TAAATTAGGA GATCTGGACT GGGAGAAACT CTCTGCTCTT 
CTGATGGACT CTCATAGCAA AGTGGAGAAA ATCAAGCTAA ATAATTGTGA GCTGACAGAG 
AAAAGCTGTT CAGTTCTAGC TGCTGTTCTC TCCTCCAAAA CCATCCTGAA AGAGCTGAAC 
CTGAACAACA GTCGTCTGCT GGACTCAGGA GTCAGAAAGA TCTGTGAGGG ACTGAAGAAT 
CCTGTGTGTG AGCTGAAGAT ACTCAATCTT TCAGACTGCA GTATTACAGA AGAAGGTTAT 
GAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTGCACTGA AGACACTGAG ATTTTTAAGA AGTCCTGCTG CTGATGAAGG CTGTCAGTTA 
GTAACTGGAG TTGTGGGTAA AAACCCGTTA CTCCTGAAGG AACTGAATCT GAGTGATCAT 
GAGCTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGCCAG 
ATCAACACAC TCAATCTGAG TTATTGCAGT ATTACAGAGA AACAGTGTGA GATCCTGACT 
TCAGCTCTGA AATCAAACCC ATCACACCTG AGAGAACTGG ACCTCAGTGG GAATGAACTA 
GGAGACTTTC AAAGCCTTGG CTCTCTACTG AAGAACCAAC AATTCAAGCT GGAGAAACTA 
ATTCTGTGTG TTTGCAGTAT TAAAGAGAAG CAGTGTGAGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGGA ATGAACTCAA GCACAGAAGA 
GTGAAGCAAT TTTTTGATGT GCTGAAAAAA TCAACCTGTA AACTGGAGCG ACTGAGGTTA 
AGATACTGTA GTATACAAGC CGATGATTGT ACTGATCTGG CTTCAGCTCT GAAATCAAAC 
CCATCCCACC TGAGAGAACT GGACCTCAGT GGAAACAATC TAGGAGACTC TGGAGTGAAA 
AAACTCAGTG ATTTACTGAT AAACCAACAA TTCAAGCTGG AGAAACTACG TCTGAGTTAT 
TGCAGTATTA CAGAGAAACA GTGTGTGATC CTGACTTCAG CTCTGAAATC AAACCCATCA 
CACCTGAGAG AACTGAACCT CAATGGGAAT AGACTAGGAG ACTCTGGAGC TGATGGCCTT 
AACCATCTGC TGAGCAGTGT GGATTGCAAG CTAAATAAAC TACATCTGTG TGATTGCAGT 
ATTACAGAGA AACAGTTGCT CATCCCAATC ACAGCTACGT GTATGGGCCT ATTACACCTG 
AGAGAACTGG ACCTCAGTAA GAACCAAATA AAAAACCAAG GAGTGTACAT GCTGTGTGAT 
GTGCTGAAAA ACCCACGCTG TAGACTCAAG AGGTTAAGAC TAAATGACTG TGGCATTACA 
GATGTTACAC CCTTAACTCA GTCTTTGACC AAGGACAAAG CATTGAAGTT CTTAAAAGAG 
CTTGAACTGA GTAAAAATCA AATGGGATTC TTCGAAAAGC AAAAACTCAG TGACCTGCTG 
CGAGACTCAA ACTGTAAACT GAGGCTTGAG GAAGAAGAAG GTTATTTTAG AGCTGGTGTA 
AAACGGGTTA CTACATTTTT GATGCCTCCA TGGTCAAAAG TTCAAAAACC TGTGGATCTC 
ACAGGAGAGG AAAGTTCATC ATCAGATGAG GACGCCTCTG AAGATGAGAC TGATCAGGCA 
GAGGAGGCTG ATAAGCTCTC AAATGAGGCG GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_021469423.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325098.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X3, mRNA.

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CloneID	ODa151246
Clone ID Related Accession (Same CDS sequence)	XM_021469423.1
Accession Version	XM_021469423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein si:ch211-11p18.6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CTGCTGATGA AGCCTGTCAG TTTGTGACTG GAATTGTGGG TAAAAACCTG 
TTACTCCTGA AAGAACTGAA TCTGAGTGAT CGTCAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACACTCAACA CACTCATGCT GAATAATAAC 
AGGATTACAG CAGAAGGTTG TGCTGCTTTG ACTTCAGCAT TTAATTTAAA TCCATCAAAC 
CTGATGGAGC TCACTCTGAG TGGAAATAAA CTAGAAGACT CAGGAGTAGA AAAGATCTGT 
TCTCTGCTGA AAAATACACA GTGCAGATTG AAGAAACTGA GTCTTTCAGA CTGCAGGATC 
ACTGAAGAAG GATATAAAAG TTTGACTTTG TCCCTGAAGT CAAATTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACAA 
CGGGGTGATT GCTGTAAATT AAAAACCGTC AGGTTTTTAA AGAGTTCTGC TGCACAGGAA 
GCGTGTGACT ATCTTACTAA AGCTGTGGGT AAAAGTCCAT TATTACTGAC AAAACTGGAT 
CTGAGTGAAG TTAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGAAGGAC 
TCTCATAGCA AAGTGGAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT 
TCAGTTCTAG CTGCTGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC 
AGTCGTCTGC TGGACTCAGG AGTCAGAGAG ATCTGTGAGG GGCTGAAGAA TCCTGTGTGT 
GAGCTGAAGA TACTCAACCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC TTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GAAGCTCACT GACTTACTGC AGGATCCACA CTGTACCCTG 
AATACACTGA GGTTTTTGAG TCCTGCCGCA GAAGAAGCCT GTCAGTTTGT GACTGGAATT 
GTGGGTAAAA ACCCGATACT TCTGAAAGAA CTGAATCTGA GTGATCATGA ACTAGGAGAC 
ACACGAGTGA ATCAACTCTC TGCTCTACTG CAGGATAAAC ACTGTAAACT CAACACACTG 
ATTCTGAAAA ACAGCTTTAT CTCAGAGGAA GATTGTCGTG TTCTGGCTGA AGCTTTTAAT 
TCAAACCCAT CAAATCTGAG AGAGCTGAAT TTGAATGGGA CTAAACTCAC AAACTCAGGA 
ATGAAGAGCT TCAACACTCT GTTTCAAAAC CAACAGTGCA GACTGGAAAA GCTGAAGTTT 
AGCGGCATCA GTATCACAGC AGAAGGTTGT GCTGCTTTGA CTTCAGCATT TAGCTCAAAC 
CCGTCACACC TGATAGAGCT AAATCTGAGT GGGAATACAC TAGAAGACTC AGGAGTAACA 
GAAATCTCCA GTCTACTGGC AAACTCACAG TGCATGCTGA AGATACTCAG TCTTTCAGAC 
TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC 
ACTGCTTTGA TTCCAGAGTG TGCTCTGAAA ACATTGAGGT TTTTGGGTCC TGCAGCAGAA 
GAAGCCTGTC AGTTTGTGAC TGGAATTGTG GGTAAAAACC CGTTACTCCT GAAAGAACTG 
AATCTGAGTG ATCGTAAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GTAAACCCAA CACACTTCAG CTGAATAATA ACAGGATTAC AGCAGAAGGT 
TGTGCTGCTT TAACTTCAGC ATTTAATTTA AACCCTTCAC ACCTAATAGA GCTCAATCTG 
AGTGGGAATA AACTAAGAGA CTCAGGAGTA GAGGAGATCT GTCCTCTGCT GAAAAATACA 
CAGTGCAGAC TGGAGAGACT AAGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTAAAGGAG CTCACTGACT TACTGCAGGA TGAGCGATAT 
AGATTGAAGA CCCTCAGGCT TTTGAAAACT ACTGCAGCAC AAGAAGCGTG TGACTATCTT 
ACTAAAGTTG TGGGTAAAAG TCCATTATTA CTGACAGAAC TGGATCTGAG TGAAGATAAA 
TTAGGAGATC TGGATGGAGA GAAACTCTCT GCTCTTCTGA TGGACTCTCA TAGTAAAGTG 
GAGAAAATCA AGCTGAATAA TTGTAAGCAG ATTGAAAAAG GCTGTTCAGT ACTAGCTGCC 
GTTCTCTCCT CAAAAACAAT CCTAAAAGAG ATGAACCTGA ACAACAGTCG TCTGCTGGAC 
TCAGGAGTCT TAGAGATCTG TGAGGGACTG AAGAACCCTG TGTGTGAGCT GAAGATTCTC 
AAGCTCAGTA AGTGTGATTT AACAGAGGAA AGCTGTTCAG CTCTGGCTTC AGTTCTCAGA 
TCAGACTCCA GCAGTCTGAA GGATCTTGAC CTGAGCAATA ATAATCTGCA GGATTCAGGA 
GTGAAGTTGC TCTCTGATGG ACTAAAGCAC AGTAAACTGA AGAGACTCAG TCTTTCAGAC 
TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC 
ACTGACTTAC TGCAGGATCC ACACTGTACA CTAAAGACAC TGAGTTGTTT TAGTCCTGCT 
GCTGATGAAG GCTGTCAGTT TGTTACTGGA ATTGTGGGTA AAAACCCGTT ACTACTGAAA 
GAACTGAATC TGAGTGATCG TGAGCTAGGA GACACACGGG TGAATCAACT CTCTGCTCTA 
CTGCAAGATA AACACTGTAC ACTCAATATA CTCACGCTGA ATAATAACAG GATTACAGCA 
GAAGGTTGTG CTGCTTTGAC TTCAGCATTT AATTTAAACC CTTCACACCT GATAGAGCTC 
AATCTGAGTG GAAATAAACT AGGAGACTCA GGAGTAGAGA AGATCTGTCC TCTGCTGAAA 
AATACCCAGT GCAGATTGGA GAAATTAAAT CTTTCAGACT GCAGCATCAC TGGAAAAGGT 
TATGAAACAT TGATTTCAGC TTTGAAGTCA AACTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGGAAATC AGGAGTGCAG AAGCTCACTG ATTTGCTACG AGCTGGAAGC 
TGTAAATTAA AAACCATCAG GACTTTGAAA GGTTCCGCTG CACAGGAAGC GTGTGACTAT 
CTAACTAAAG CTGTGGGTAA AAGTCCATTA TTACTGACAG AACTGGATCT GAGTGAAGAT 
AAATTAGGAG ATCTGGATGG AGAGAAACTC TCTGCTCTTC TGATGGACTC TCATAGCAAA 
GTGGAGAAAA TCAGGATGAA TAACTGTAAA AAGCTGACAG AGAAAAGCTG TTTAGTTCTA 
GCTGCTGTTC TTTCATCCAA AACCATCTTG AAAGAGATGA ACCTAAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAAAGT GATCTGTGAG GGACTGAAGA ACTCTAAGCT GGAGATTTTA 
AAACTTTCAA ATTGTAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCTGGA 
GTAAAGGAGC TCACTGAGTT ACTGCAGAGT TCGTGGATTC CATGGAAAAA AGTGAAGTGT 
TCAATGAGGT TTTTGAGTCC TGCTGCTGAT GAATTTTGTC AGTTTGTGTC TGGAATTGTG 
GGTAAAAACC CGTTACTCCT GAAAGAACTG AATCTGAGTG ATCATGTACT AGCAGACACA 
CAAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT GCAAACTCAA CACACTCATT 
CTTTCAGACT GCAGTATCAG TGGAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACA ATCAGGAGTG 
AAAGAGCTCA CTGAGTTACT GCAGGATCCA CACTGTACAC TGAAGACACT GAGGTTTTTG 
AGTGATGATG CAGATGAAGC CTGTGAACAT TTGTATAAAG TTCTGGGTGG AAACCTGTTA 
CTCCTGAGAG AGCTGAATCT GACTAAACAT GATCTTGGAC CATCAGGATT AGAGAAACTT 
GCTGCAGTAC TGAAAGATAA ACACTGTAAA CTCAACACAC TGATTCTGAA TAACAGCACT 
ATCTCAGAGG AAGATTTTCA TGTTCTGACT GAAGCTTTTA ATTCAAACCC AACAAATCTG 
ACAGAGCTGA ACTTGAGTGG GACTAAACTC ACAAACTCAG GAATGAAGAG CTTCACAACT 
CTGTTTCAAA ACCAACAGTG TAGATTGGAA AAGCTGAAGT TCAGCAGCAT GTGTATTACA 
GCAGAAGGTT GTGCTGCTTT GACTTCAGCA TTTAACTCAA ACCCTTCACA CCTGAAAGAG 
CTCAATCTGA GTGGGAATAA ACTAGGAGAC TCAGGAGTAA GAAAAATCTC CAGTCTACTG 
AGAAACTCGC AGTGCATGCT GAAGATACTC GGTCTTTCAG ACTGCAGTAT CAGTGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT TACATCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACCATCAGGA GTGAAGGAGC TCACTGGCTT ACTACTCGAT 
CCACACTGTA CACTGACGAC ACTGAGGTTT TTGAGTCCTG CTGCTGATGA ATTCTGTCAG 
TTTGTGTCTG GAATTGTGGG TAAATACCCA TTACTCCTGA AAGAACTGAA TCTGAGTGAT 
CGTGGACTAG GGGACACACG AGTGAATCAA CTCTCTGCTC TACTGCAGGA TAAACACTTT 
AGACTCAACA CACTCATGCT GAATAATAAC AGGATTACAG CAGAAGGTTG TGCTGCTTTA 
ACTTCAGCAT TTAATTTAAA TCCTTCACAC CTGATAGAGC TCAATCTGAG TGGGAATAAA 
CTAGAAGACT CAGGAGTAGA GAAGATCTGT CCTCTGCTGG AAAATATACA ATGCCGATTA 
GAGAAACTGA GTCTTTCAGA CTGCAGTATC AGGGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGATTTA CTGCAGAGTA GAAGCTGTAA ATTCAGGTTT 
CTGAAGAGTT CTGCTGCACA GGAAGCATGC GAGTATCTTA CTAATGTTCT GGGTATAAGT 
CCATTATTAC TGACAGAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGACTGGGAG 
AAACTCTCTG CTCTTCTGAT GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTAAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGC TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AAAGATCTGT 
GAGGGACTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ATCTTTCAGA CTGCAGTATT 
ACAGAAGAAG GTTATGAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGAGTTA 
CTGCAGGATC CACACTGTGC ACTGAAGACA CTGAGATTTT TAAGAAGTCC TGCTGCTGAT 
GAAGGCTGTC AGTTAGTAAC TGGAGTTGTG GGTAAAAACC CGTTACTCCT GAAGGAACTG 
AATCTGAGTG ATCATGAGCT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GCCAGATCAA CACACTCAAT CTGAGTTATT GCAGTATTAC AGAGAAACAG 
TGTGAGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGGACCTC 
AGTGGGAATG AACTAGGAGA CTTTCAAAGC CTTGGCTCTC TACTGAAGAA CCAACAATTC 
AAGCTGGAGA AACTAATTCT GTGTGTTTGC AGTATTAAAG AGAAGCAGTG TGAGATCCTG 
ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGAACCTCAG TGGGAATGAA 
CTCAAGCACA GAAGAGTGAA GCAATTTTTT GATGTGCTGA AAAAATCAAC CTGTAAACTG 
GAGCGACTGA GGTTAAGATA CTGTAGTATA CAAGCCGATG ATTGTACTGA TCTGGCTTCA 
GCTCTGAAAT CAAACCCATC CCACCTGAGA GAACTGGACC TCAGTGGAAA CAATCTAGGA 
GACTCTGGAG TGAAAAAACT CAGTGATTTA CTGATAAACC AACAATTCAA GCTGGAGAAA 
CTACGTCTGA GTTATTGCAG TATTACAGAG AAACAGTGTG TGATCCTGAC TTCAGCTCTG 
AAATCAAACC CATCACACCT GAGAGAACTG AACCTCAATG GGAATAGACT AGGAGACTCT 
GGAGCTGATG GCCTTAACCA TCTGCTGAGC AGTGTGGATT GCAAGCTAAA TAAACTACAT 
CTGTGTGATT GCAGTATTAC AGAGAAACAG TTGCTCATCC CAATCACAGC TACGTGTATG 
GGCCTATTAC ACCTGAGAGA ACTGGACCTC AGTAAGAACC AAATAAAAAA CCAAGGAGTG 
TACATGCTGT GTGATGTGCT GAAAAACCCA CGCTGTAGAC TCAAGAGGTT AAGACTAAAT 
GACTGTGGCA TTACAGATGT TACACCCTTA ACTCAGTCTT TGACCAAGGA CAAAGCATTG 
AAGTTCTTAA AAGAGCTTGA ACTGAGTAAA AATCAAATGG GATTCTTCGA AAAGCAAAAA 
CTCAGTGACC TGCTGCGAGA CTCAAACTGT AAACTGAGGC TTGAGGAAGA AGAAGGTTAT 
TTTAGAGCTG GTGTAAAACG GGTTACTACA TTTTTGATGC CTCCATGGTC AAAAGTTCAA 
AAACCTGTGG ATCTCACAGG AGAGGAAAGT TCATCATCAG ATGAGGACGC CTCTGAAGAT 
GAGACTGATC AGGCAGAGGA GGCTGATAAG CTCTCAAATG AGGCGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325098.1
CDS	65..8275
Translation	MSNNNLQDSGVKLLCTALKNVKCELEVLRISYCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGILGKNPLLLKELNLSDCELGDTGVNQLSALLQDKHCKPKTLMLRGCGLKEETCSALATVLGSDTSLKELDMSNNNLQDLGVKKLQSGLENTNCTLEKLSLSDCNISEEGYKALASALRSNPSHLIELNLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEACQFVTGIVGKNLLLLKELNLSDRQLGDTRVNQLSALLQDKHCTLNTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLKFSGISITAEGCAALTSAFSSNPSHLIELNLSGNTLEDSGVTEISSLLANSQCMLKILSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACEHLYKVLGGNLLLLRELNLTKHDLGPSGLEKLAAVLKDKHCKLNTLILNNSTISEEDFHVLTEAFNSNPTNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_021469423.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469423.1

ORF Insert Sequence:

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CTGCTGATGA AGCCTGTCAG TTTGTGACTG GAATTGTGGG TAAAAACCTG 
TTACTCCTGA AAGAACTGAA TCTGAGTGAT CGTCAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACACTCAACA CACTCATGCT GAATAATAAC 
AGGATTACAG CAGAAGGTTG TGCTGCTTTG ACTTCAGCAT TTAATTTAAA TCCATCAAAC 
CTGATGGAGC TCACTCTGAG TGGAAATAAA CTAGAAGACT CAGGAGTAGA AAAGATCTGT 
TCTCTGCTGA AAAATACACA GTGCAGATTG AAGAAACTGA GTCTTTCAGA CTGCAGGATC 
ACTGAAGAAG GATATAAAAG TTTGACTTTG TCCCTGAAGT CAAATTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACAA 
CGGGGTGATT GCTGTAAATT AAAAACCGTC AGGTTTTTAA AGAGTTCTGC TGCACAGGAA 
GCGTGTGACT ATCTTACTAA AGCTGTGGGT AAAAGTCCAT TATTACTGAC AAAACTGGAT 
CTGAGTGAAG TTAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGAAGGAC 
TCTCATAGCA AAGTGGAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT 
TCAGTTCTAG CTGCTGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC 
AGTCGTCTGC TGGACTCAGG AGTCAGAGAG ATCTGTGAGG GGCTGAAGAA TCCTGTGTGT 
GAGCTGAAGA TACTCAACCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC TTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GAAGCTCACT GACTTACTGC AGGATCCACA CTGTACCCTG 
AATACACTGA GGTTTTTGAG TCCTGCCGCA GAAGAAGCCT GTCAGTTTGT GACTGGAATT 
GTGGGTAAAA ACCCGATACT TCTGAAAGAA CTGAATCTGA GTGATCATGA ACTAGGAGAC 
ACACGAGTGA ATCAACTCTC TGCTCTACTG CAGGATAAAC ACTGTAAACT CAACACACTG 
ATTCTGAAAA ACAGCTTTAT CTCAGAGGAA GATTGTCGTG TTCTGGCTGA AGCTTTTAAT 
TCAAACCCAT CAAATCTGAG AGAGCTGAAT TTGAATGGGA CTAAACTCAC AAACTCAGGA 
ATGAAGAGCT TCAACACTCT GTTTCAAAAC CAACAGTGCA GACTGGAAAA GCTGAAGTTT 
AGCGGCATCA GTATCACAGC AGAAGGTTGT GCTGCTTTGA CTTCAGCATT TAGCTCAAAC 
CCGTCACACC TGATAGAGCT AAATCTGAGT GGGAATACAC TAGAAGACTC AGGAGTAACA 
GAAATCTCCA GTCTACTGGC AAACTCACAG TGCATGCTGA AGATACTCAG TCTTTCAGAC 
TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC 
ACTGCTTTGA TTCCAGAGTG TGCTCTGAAA ACATTGAGGT TTTTGGGTCC TGCAGCAGAA 
GAAGCCTGTC AGTTTGTGAC TGGAATTGTG GGTAAAAACC CGTTACTCCT GAAAGAACTG 
AATCTGAGTG ATCGTAAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GTAAACCCAA CACACTTCAG CTGAATAATA ACAGGATTAC AGCAGAAGGT 
TGTGCTGCTT TAACTTCAGC ATTTAATTTA AACCCTTCAC ACCTAATAGA GCTCAATCTG 
AGTGGGAATA AACTAAGAGA CTCAGGAGTA GAGGAGATCT GTCCTCTGCT GAAAAATACA 
CAGTGCAGAC TGGAGAGACT AAGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTAAAGGAG CTCACTGACT TACTGCAGGA TGAGCGATAT 
AGATTGAAGA CCCTCAGGCT TTTGAAAACT ACTGCAGCAC AAGAAGCGTG TGACTATCTT 
ACTAAAGTTG TGGGTAAAAG TCCATTATTA CTGACAGAAC TGGATCTGAG TGAAGATAAA 
TTAGGAGATC TGGATGGAGA GAAACTCTCT GCTCTTCTGA TGGACTCTCA TAGTAAAGTG 
GAGAAAATCA AGCTGAATAA TTGTAAGCAG ATTGAAAAAG GCTGTTCAGT ACTAGCTGCC 
GTTCTCTCCT CAAAAACAAT CCTAAAAGAG ATGAACCTGA ACAACAGTCG TCTGCTGGAC 
TCAGGAGTCT TAGAGATCTG TGAGGGACTG AAGAACCCTG TGTGTGAGCT GAAGATTCTC 
AAGCTCAGTA AGTGTGATTT AACAGAGGAA AGCTGTTCAG CTCTGGCTTC AGTTCTCAGA 
TCAGACTCCA GCAGTCTGAA GGATCTTGAC CTGAGCAATA ATAATCTGCA GGATTCAGGA 
GTGAAGTTGC TCTCTGATGG ACTAAAGCAC AGTAAACTGA AGAGACTCAG TCTTTCAGAC 
TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC 
ACTGACTTAC TGCAGGATCC ACACTGTACA CTAAAGACAC TGAGTTGTTT TAGTCCTGCT 
GCTGATGAAG GCTGTCAGTT TGTTACTGGA ATTGTGGGTA AAAACCCGTT ACTACTGAAA 
GAACTGAATC TGAGTGATCG TGAGCTAGGA GACACACGGG TGAATCAACT CTCTGCTCTA 
CTGCAAGATA AACACTGTAC ACTCAATATA CTCACGCTGA ATAATAACAG GATTACAGCA 
GAAGGTTGTG CTGCTTTGAC TTCAGCATTT AATTTAAACC CTTCACACCT GATAGAGCTC 
AATCTGAGTG GAAATAAACT AGGAGACTCA GGAGTAGAGA AGATCTGTCC TCTGCTGAAA 
AATACCCAGT GCAGATTGGA GAAATTAAAT CTTTCAGACT GCAGCATCAC TGGAAAAGGT 
TATGAAACAT TGATTTCAGC TTTGAAGTCA AACTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGGAAATC AGGAGTGCAG AAGCTCACTG ATTTGCTACG AGCTGGAAGC 
TGTAAATTAA AAACCATCAG GACTTTGAAA GGTTCCGCTG CACAGGAAGC GTGTGACTAT 
CTAACTAAAG CTGTGGGTAA AAGTCCATTA TTACTGACAG AACTGGATCT GAGTGAAGAT 
AAATTAGGAG ATCTGGATGG AGAGAAACTC TCTGCTCTTC TGATGGACTC TCATAGCAAA 
GTGGAGAAAA TCAGGATGAA TAACTGTAAA AAGCTGACAG AGAAAAGCTG TTTAGTTCTA 
GCTGCTGTTC TTTCATCCAA AACCATCTTG AAAGAGATGA ACCTAAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAAAGT GATCTGTGAG GGACTGAAGA ACTCTAAGCT GGAGATTTTA 
AAACTTTCAA ATTGTAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCTGGA 
GTAAAGGAGC TCACTGAGTT ACTGCAGAGT TCGTGGATTC CATGGAAAAA AGTGAAGTGT 
TCAATGAGGT TTTTGAGTCC TGCTGCTGAT GAATTTTGTC AGTTTGTGTC TGGAATTGTG 
GGTAAAAACC CGTTACTCCT GAAAGAACTG AATCTGAGTG ATCATGTACT AGCAGACACA 
CAAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT GCAAACTCAA CACACTCATT 
CTTTCAGACT GCAGTATCAG TGGAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACA ATCAGGAGTG 
AAAGAGCTCA CTGAGTTACT GCAGGATCCA CACTGTACAC TGAAGACACT GAGGTTTTTG 
AGTGATGATG CAGATGAAGC CTGTGAACAT TTGTATAAAG TTCTGGGTGG AAACCTGTTA 
CTCCTGAGAG AGCTGAATCT GACTAAACAT GATCTTGGAC CATCAGGATT AGAGAAACTT 
GCTGCAGTAC TGAAAGATAA ACACTGTAAA CTCAACACAC TGATTCTGAA TAACAGCACT 
ATCTCAGAGG AAGATTTTCA TGTTCTGACT GAAGCTTTTA ATTCAAACCC AACAAATCTG 
ACAGAGCTGA ACTTGAGTGG GACTAAACTC ACAAACTCAG GAATGAAGAG CTTCACAACT 
CTGTTTCAAA ACCAACAGTG TAGATTGGAA AAGCTGAAGT TCAGCAGCAT GTGTATTACA 
GCAGAAGGTT GTGCTGCTTT GACTTCAGCA TTTAACTCAA ACCCTTCACA CCTGAAAGAG 
CTCAATCTGA GTGGGAATAA ACTAGGAGAC TCAGGAGTAA GAAAAATCTC CAGTCTACTG 
AGAAACTCGC AGTGCATGCT GAAGATACTC GGTCTTTCAG ACTGCAGTAT CAGTGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT TACATCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACCATCAGGA GTGAAGGAGC TCACTGGCTT ACTACTCGAT 
CCACACTGTA CACTGACGAC ACTGAGGTTT TTGAGTCCTG CTGCTGATGA ATTCTGTCAG 
TTTGTGTCTG GAATTGTGGG TAAATACCCA TTACTCCTGA AAGAACTGAA TCTGAGTGAT 
CGTGGACTAG GGGACACACG AGTGAATCAA CTCTCTGCTC TACTGCAGGA TAAACACTTT 
AGACTCAACA CACTCATGCT GAATAATAAC AGGATTACAG CAGAAGGTTG TGCTGCTTTA 
ACTTCAGCAT TTAATTTAAA TCCTTCACAC CTGATAGAGC TCAATCTGAG TGGGAATAAA 
CTAGAAGACT CAGGAGTAGA GAAGATCTGT CCTCTGCTGG AAAATATACA ATGCCGATTA 
GAGAAACTGA GTCTTTCAGA CTGCAGTATC AGGGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGATTTA CTGCAGAGTA GAAGCTGTAA ATTCAGGTTT 
CTGAAGAGTT CTGCTGCACA GGAAGCATGC GAGTATCTTA CTAATGTTCT GGGTATAAGT 
CCATTATTAC TGACAGAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGACTGGGAG 
AAACTCTCTG CTCTTCTGAT GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTAAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGC TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AAAGATCTGT 
GAGGGACTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ATCTTTCAGA CTGCAGTATT 
ACAGAAGAAG GTTATGAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGAGTTA 
CTGCAGGATC CACACTGTGC ACTGAAGACA CTGAGATTTT TAAGAAGTCC TGCTGCTGAT 
GAAGGCTGTC AGTTAGTAAC TGGAGTTGTG GGTAAAAACC CGTTACTCCT GAAGGAACTG 
AATCTGAGTG ATCATGAGCT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GCCAGATCAA CACACTCAAT CTGAGTTATT GCAGTATTAC AGAGAAACAG 
TGTGAGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGGACCTC 
AGTGGGAATG AACTAGGAGA CTTTCAAAGC CTTGGCTCTC TACTGAAGAA CCAACAATTC 
AAGCTGGAGA AACTAATTCT GTGTGTTTGC AGTATTAAAG AGAAGCAGTG TGAGATCCTG 
ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGAACCTCAG TGGGAATGAA 
CTCAAGCACA GAAGAGTGAA GCAATTTTTT GATGTGCTGA AAAAATCAAC CTGTAAACTG 
GAGCGACTGA GGTTAAGATA CTGTAGTATA CAAGCCGATG ATTGTACTGA TCTGGCTTCA 
GCTCTGAAAT CAAACCCATC CCACCTGAGA GAACTGGACC TCAGTGGAAA CAATCTAGGA 
GACTCTGGAG TGAAAAAACT CAGTGATTTA CTGATAAACC AACAATTCAA GCTGGAGAAA 
CTACGTCTGA GTTATTGCAG TATTACAGAG AAACAGTGTG TGATCCTGAC TTCAGCTCTG 
AAATCAAACC CATCACACCT GAGAGAACTG AACCTCAATG GGAATAGACT AGGAGACTCT 
GGAGCTGATG GCCTTAACCA TCTGCTGAGC AGTGTGGATT GCAAGCTAAA TAAACTACAT 
CTGTGTGATT GCAGTATTAC AGAGAAACAG TTGCTCATCC CAATCACAGC TACGTGTATG 
GGCCTATTAC ACCTGAGAGA ACTGGACCTC AGTAAGAACC AAATAAAAAA CCAAGGAGTG 
TACATGCTGT GTGATGTGCT GAAAAACCCA CGCTGTAGAC TCAAGAGGTT AAGACTAAAT 
GACTGTGGCA TTACAGATGT TACACCCTTA ACTCAGTCTT TGACCAAGGA CAAAGCATTG 
AAGTTCTTAA AAGAGCTTGA ACTGAGTAAA AATCAAATGG GATTCTTCGA AAAGCAAAAA 
CTCAGTGACC TGCTGCGAGA CTCAAACTGT AAACTGAGGC TTGAGGAAGA AGAAGGTTAT 
TTTAGAGCTG GTGTAAAACG GGTTACTACA TTTTTGATGC CTCCATGGTC AAAAGTTCAA 
AAACCTGTGG ATCTCACAGG AGAGGAAAGT TCATCATCAG ATGAGGACGC CTCTGAAGAT 
GAGACTGATC AGGCAGAGGA GGCTGATAAG CTCTCAAATG AGGCGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_021469424.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325099.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X4, mRNA.

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CloneID	ODa151247
Clone ID Related Accession (Same CDS sequence)	XM_021469424.1
Accession Version	XM_021469424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein si:ch211-11p18.6 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAGTAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTAAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TGATAGAGTT ACTGCAGGAT CCACTCTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CTGCTGATGA AGCCTGTCAG TTTGTGACTG GAATTGTGGG TAAAAACCTG 
TTACTCCTGA AAGAACTGAA TCTGAGTGAT CGTCAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACACTCAACA CACTCATGCT GAATAATAAC 
AGGATTACAG CAGAAGGTTG TGCTGCTTTG ACTTCAGCAT TTAATTTAAA TCCATCAAAC 
CTGATGGAGC TCACTCTGAG TGGAAATAAA CTAGAAGACT CAGGAGTAGA AAAGATCTGT 
TCTCTGCTGA AAAATACACA GTGCAGATTG AAGAAACTGA GTCTTTCAGA CTGCAGGATC 
ACTGAAGAAG GATATAAAAG TTTGACTTTG TCCCTGAAGT CAAATTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACAA 
CGGGGTGATT GCTGTAAATT AAAAACCGTC AGGTTTTTAA AGAGTTCTGC TGCACAGGAA 
GCGTGTGACT ATCTTACTAA AGCTGTGGGT AAAAGTCCAT TATTACTGAC AAAACTGGAT 
CTGAGTGAAG TTAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGAAGGAC 
TCTCATAGCA AAGTGGAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT 
TCAGTTCTAG CTGCTGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC 
AGTCGTCTGC TGGACTCAGG AGTCAGAGAG ATCTGTGAGG GGCTGAAGAA TCCTGTGTGT 
GAGCTGAAGA TACTCAACCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC TTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GAAGCTCACT GACTTACTGC AGGATCCACA CTGTACCCTG 
AATACACTGA GGTTTTTGAG TCCTGCCGCA GAAGAAGCCT GTCAGTTTGT GACTGGAATT 
GTGGGTAAAA ACCCGATACT TCTGAAAGAA CTGAATCTGA GTGATCATGA ACTAGGAGAC 
ACACGAGTGA ATCAACTCTC TGCTCTACTG CAGGATAAAC ACTGTAAACT CAACACACTG 
ATTCTGAAAA ACAGCTTTAT CTCAGAGGAA GATTGTCGTG TTCTGGCTGA AGCTTTTAAT 
TCAAACCCAT CAAATCTGAG AGAGCTGAAT TTGAATGGGA CTAAACTCAC AAACTCAGGA 
ATGAAGAGCT TCAACACTCT GTTTCAAAAC CAACAGTGCA GACTGGAAAA GCTGAAGTTT 
AGCGGCATCA GTATCACAGC AGAAGGTTGT GCTGCTTTGA CTTCAGCATT TAGCTCAAAC 
CCGTCACACC TGATAGAGCT AAATCTGAGT GGGAATACAC TAGAAGACTC AGGAGTAACA 
GAAATCTCCA GTCTACTGGC AAACTCACAG TGCATGCTGA AGATACTCAG TCTTTCAGAC 
TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC 
ACTGCTTTGA TTCCAGAGTG TGCTCTGAAA ACATTGAGGT TTTTGGGTCC TGCAGCAGAA 
GAAGCCTGTC AGTTTGTGAC TGGAATTGTG GGTAAAAACC CGTTACTCCT GAAAGAACTG 
AATCTGAGTG ATCGTAAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GTAAACCCAA CACACTTCAG CTGAATAATA ACAGGATTAC AGCAGAAGGT 
TGTGCTGCTT TAACTTCAGC ATTTAATTTA AACCCTTCAC ACCTAATAGA GCTCAATCTG 
AGTGGGAATA AACTAAGAGA CTCAGGAGTA GAGGAGATCT GTCCTCTGCT GAAAAATACA 
CAGTGCAGAC TGGAGAGACT AAGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTAAAGGAG CTCACTGACT TACTGCAGGA TGAGCGATAT 
AGATTGAAGA CCCTCAGGCT TTTGAAAACT ACTGCAGCAC AAGAAGCGTG TGACTATCTT 
ACTAAAGTTG TGGGTAAAAG TCCATTATTA CTGACAGAAC TGGATCTGAG TGAAGATAAA 
TTAGGAGATC TGGATGGAGA GAAACTCTCT GCTCTTCTGA TGGACTCTCA TAGTAAAGTG 
GAGAAAATCA AGCTGAATAA TTGTAAGCAG ATTGAAAAAG GCTGTTCAGT ACTAGCTGCC 
GTTCTCTCCT CAAAAACAAT CCTAAAAGAG ATGAACCTGA ACAACAGTCG TCTGCTGGAC 
TCAGGAGTCT TAGAGATCTG TGAGGGACTG AAGAACCCTG TGTGTGAGCT GAAGATTCTC 
AAGCTCAGTA AGTGTGATTT AACAGAGGAA AGCTGTTCAG CTCTGGCTTC AGTTCTCAGA 
TCAGACTCCA GCAGTCTGAA GGATCTTGAC CTGAGCAATA ATAATCTGCA GGATTCAGGA 
GTGAAGTTGC TCTCTGATGG ACTAAAGCAC AGTAAACTGA AGAGACTCAG TCTTTCAGAC 
TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC 
ACTGACTTAC TGCAGGATCC ACACTGTACA CTAAAGACAC TGAGTTGTTT TAGTCCTGCT 
GCTGATGAAG GCTGTCAGTT TGTTACTGGA ATTGTGGGTA AAAACCCGTT ACTACTGAAA 
GAACTGAATC TGAGTGATCG TGAGCTAGGA GACACACGGG TGAATCAACT CTCTGCTCTA 
CTGCAAGATA AACACTGTAC ACTCAATATA CTCACGCTGA ATAATAACAG GATTACAGCA 
GAAGGTTGTG CTGCTTTGAC TTCAGCATTT AATTTAAACC CTTCACACCT GATAGAGCTC 
AATCTGAGTG GAAATAAACT AGGAGACTCA GGAGTAGAGA AGATCTGTCC TCTGCTGAAA 
AATACCCAGT GCAGATTGGA GAAATTAAAT CTTTCAGACT GCAGCATCAC TGGAAAAGGT 
TATGAAACAT TGATTTCAGC TTTGAAGTCA AACTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGGAAATC AGGAGTGCAG AAGCTCACTG ATTTGCTACG AGCTGGAAGC 
TGTAAATTAA AAACCATCAG GACTTTGAAA GGTTCCGCTG CACAGGAAGC GTGTGACTAT 
CTAACTAAAG CTGTGGGTAA AAGTCCATTA TTACTGACAG AACTGGATCT GAGTGAAGAT 
AAATTAGGAG ATCTGGATGG AGAGAAACTC TCTGCTCTTC TGATGGACTC TCATAGCAAA 
GTGGAGAAAA TCAGGATGAA TAACTGTAAA AAGCTGACAG AGAAAAGCTG TTTAGTTCTA 
GCTGCTGTTC TTTCATCCAA AACCATCTTG AAAGAGATGA ACCTAAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAAAGT GATCTGTGAG GGACTGAAGA ACTCTAAGCT GGAGATTTTA 
AAACTTTCAA ATTGTAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCTGGA 
GTAAAGGAGC TCACTGAGTT ACTGCAGAGT TCGTGGATTC CATGGAAAAA AGTGAAGTGT 
TCAATGAGGT TTTTGAGTCC TGCTGCTGAT GAATTTTGTC AGTTTGTGTC TGGAATTGTG 
GGTAAAAACC CGTTACTCCT GAAAGAACTG AATCTGAGTG ATCATGTACT AGCAGACACA 
CAAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT GCAAACTCAA CACACTCATT 
CTTTCAGACT GCAGTATCAG TGGAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACA ATCAGGAGTG 
AAAGAGCTCA CTGAGTTACT GCAGGATCCA CACTGTACAC TGAAGACACT GAGGTTTTTG 
AGTGATGATG CAGATGAAGC CTGTGAACAT TTGTATAAAG TTCTGGGTGG AAACCTGTTA 
CTCCTGAGAG AGCTGAATCT GACTAAACAT GATCTTGGAC CATCAGGATT AGAGAAACTT 
GCTGCAGTAC TGAAAGATAA ACACTGTAAA CTCAACACAC TGATTCTGAA TAACAGCACT 
ATCTCAGAGG AAGATTTTCA TGTTCTGACT GAAGCTTTTA ATTCAAACCC AACAAATCTG 
ACAGAGCTGA ACTTGAGTGG GACTAAACTC ACAAACTCAG GAATGAAGAG CTTCACAACT 
CTGTTTCAAA ACCAACAGTG TAGATTGGAA AAGCTGAAGT TCAGCAGCAT GTGTATTACA 
GCAGAAGGTT GTGCTGCTTT GACTTCAGCA TTTAACTCAA ACCCTTCACA CCTGAAAGAG 
CTCAATCTGA GTGGGAATAA ACTAGGAGAC TCAGGAGTAA GAAAAATCTC CAGTCTACTG 
AGAAACTCGC AGTGCATGCT GAAGATACTC GGTCTTTCAG ACTGCAGTAT CAGTGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT TACATCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACCATCAGGA GTGAAGGAGC TCACTGGCTT ACTACTCGAT 
CCACACTGTA CACTGACGAC ACTGAGGTTT TTGAGTCCTG CTGCTGATGA ATTCTGTCAG 
TTTGTGTCTG GAATTGTGGG TAAATACCCA TTACTCCTGA AAGAACTGAA TCTGAGTGAT 
CGTGGACTAG GGGACACACG AGTGAATCAA CTCTCTGCTC TACTGCAGGA TAAACACTTT 
AGACTCAACA CACTCATGCT GAATAATAAC AGGATTACAG CAGAAGGTTG TGCTGCTTTA 
ACTTCAGCAT TTAATTTAAA TCCTTCACAC CTGATAGAGC TCAATCTGAG TGGGAATAAA 
CTAGAAGACT CAGGAGTAGA GAAGATCTGT CCTCTGCTGG AAAATATACA ATGCCGATTA 
GAGAAACTGA GTCTTTCAGA CTGCAGTATC AGGGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGATTTA CTGCAGAGTA GAAGCTGTAA ATTCAGGTTT 
CTGAAGAGTT CTGCTGCACA GGAAGCATGC GAGTATCTTA CTAATGTTCT GGGTATAAGT 
CCATTATTAC TGACAGAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGACTGGGAG 
AAACTCTCTG CTCTTCTGAT GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTAAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGC TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AAAGATCTGT 
GAGGGACTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ATCTTTCAGA CTGCAGTATT 
ACAGAAGAAG GTTATGAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGAGTTA 
CTGCAGGATC CACACTGTGC ACTGAAGACA CTGAGATTTT TAAGAAGTCC TGCTGCTGAT 
GAAGGCTGTC AGTTAGTAAC TGGAGTTGTG GGTAAAAACC CGTTACTCCT GAAGGAACTG 
AATCTGAGTG ATCATGAGCT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GCCAGATCAA CACACTCAAT CTGAGTTATT GCAGTATTAC AGAGAAACAG 
TGTGAGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGGACCTC 
AGTGGGAATG AACTAGGAGA CTTTCAAAGC CTTGGCTCTC TACTGAAGAA CCAACAATTC 
AAGCTGGAGA AACTAATTCT GTGTGTTTGC AGTATTAAAG AGAAGCAGTG TGAGATCCTG 
ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGAACCTCAG TGGGAATGAA 
CTCAAGCACA GAAGAGTGAA GCAATTTTTT GATGTGCTGA AAAAATCAAC CTGTAAACTG 
GAGCGACTGA GGTTAAGATA CTGTAGTATA CAAGCCGATG ATTGTACTGA TCTGGCTTCA 
GCTCTGAAAT CAAACCCATC CCACCTGAGA GAACTGGACC TCAGTGGAAA CAATCTAGGA 
GACTCTGGAG TGAAAAAACT CAGTGATTTA CTGATAAACC AACAATTCAA GCTGGAGAAA 
CTACGTCTGA GTTATTGCAG TATTACAGAG AAACAGTGTG TGATCCTGAC TTCAGCTCTG 
AAATCAAACC CATCACACCT GAGAGAACTG AACCTCAATG GGAATAGACT AGGAGACTCT 
GGAGCTGATG GCCTTAACCA TCTGCTGAGC AGTGTGGATT GCAAGCTAAA TAAACTACAT 
CTGTGTGATT GCAGTATTAC AGAGAAACAG TTGCTCATCC CAATCACAGC TACGTGTATG 
GGCCTATTAC ACCTGAGAGA ACTGGACCTC AGTAAGAACC AAATAAAAAA CCAAGGAGTG 
TACATGCTGT GTGATGTGCT GAAAAACCCA CGCTGTAGAC TCAAGAGGTT AAGACTAAAT 
GACTGTGGCA TTACAGATGT TACACCCTTA ACTCAGTCTT TGACCAAGGA CAAAGCATTG 
AAGTTCTTAA AAGAGCTTGA ACTGAGTAAA AATCAAATGG GATTCTTCGA AAAGCAAAAA 
CTCAGTGACC TGCTGCGAGA CTCAAACTGT AAACTGAGGC TTGAGGAAGA AGAAGGTTAT 
TTTAGAGCTG GTGTAAAACG GGTTACTACA TTTTTGATGC CTCCATGGTC AAAAGTTCAA 
AAACCTGTGG ATCTCACAGG AGAGGAAAGT TCATCATCAG ATGAGGACGC CTCTGAAGAT 
GAGACTGATC AGGCAGAGGA GGCTGATAAG CTCTCAAATG AGGCGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325099.1
CDS	66..8276
Translation	MSNNNLQDSGVKLLCTALKNVKCELEVLRISYCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGILGKNPLLLKELNLSDCELGDTGVNQLSALLQDKHCKPKTLMLRGCGLKEETCSALATVLGSDTSLKELDMSNNNLQDLGVKKLQSGLENTNCTLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELIELLQDPLCTLKTLRFLSPAADEACQFVTGIVGKNLLLLKELNLSDRQLGDTRVNQLSALLQDKHCTLNTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLKFSGISITAEGCAALTSAFSSNPSHLIELNLSGNTLEDSGVTEISSLLANSQCMLKILSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACEHLYKVLGGNLLLLRELNLTKHDLGPSGLEKLAAVLKDKHCKLNTLILNNSTISEEDFHVLTEAFNSNPTNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_021469424.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469424.1

ORF Insert Sequence:

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAGTAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTAAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TGATAGAGTT ACTGCAGGAT CCACTCTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CTGCTGATGA AGCCTGTCAG TTTGTGACTG GAATTGTGGG TAAAAACCTG 
TTACTCCTGA AAGAACTGAA TCTGAGTGAT CGTCAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACACTCAACA CACTCATGCT GAATAATAAC 
AGGATTACAG CAGAAGGTTG TGCTGCTTTG ACTTCAGCAT TTAATTTAAA TCCATCAAAC 
CTGATGGAGC TCACTCTGAG TGGAAATAAA CTAGAAGACT CAGGAGTAGA AAAGATCTGT 
TCTCTGCTGA AAAATACACA GTGCAGATTG AAGAAACTGA GTCTTTCAGA CTGCAGGATC 
ACTGAAGAAG GATATAAAAG TTTGACTTTG TCCCTGAAGT CAAATTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACAA 
CGGGGTGATT GCTGTAAATT AAAAACCGTC AGGTTTTTAA AGAGTTCTGC TGCACAGGAA 
GCGTGTGACT ATCTTACTAA AGCTGTGGGT AAAAGTCCAT TATTACTGAC AAAACTGGAT 
CTGAGTGAAG TTAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGAAGGAC 
TCTCATAGCA AAGTGGAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT 
TCAGTTCTAG CTGCTGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC 
AGTCGTCTGC TGGACTCAGG AGTCAGAGAG ATCTGTGAGG GGCTGAAGAA TCCTGTGTGT 
GAGCTGAAGA TACTCAACCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC TTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GAAGCTCACT GACTTACTGC AGGATCCACA CTGTACCCTG 
AATACACTGA GGTTTTTGAG TCCTGCCGCA GAAGAAGCCT GTCAGTTTGT GACTGGAATT 
GTGGGTAAAA ACCCGATACT TCTGAAAGAA CTGAATCTGA GTGATCATGA ACTAGGAGAC 
ACACGAGTGA ATCAACTCTC TGCTCTACTG CAGGATAAAC ACTGTAAACT CAACACACTG 
ATTCTGAAAA ACAGCTTTAT CTCAGAGGAA GATTGTCGTG TTCTGGCTGA AGCTTTTAAT 
TCAAACCCAT CAAATCTGAG AGAGCTGAAT TTGAATGGGA CTAAACTCAC AAACTCAGGA 
ATGAAGAGCT TCAACACTCT GTTTCAAAAC CAACAGTGCA GACTGGAAAA GCTGAAGTTT 
AGCGGCATCA GTATCACAGC AGAAGGTTGT GCTGCTTTGA CTTCAGCATT TAGCTCAAAC 
CCGTCACACC TGATAGAGCT AAATCTGAGT GGGAATACAC TAGAAGACTC AGGAGTAACA 
GAAATCTCCA GTCTACTGGC AAACTCACAG TGCATGCTGA AGATACTCAG TCTTTCAGAC 
TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC 
ACTGCTTTGA TTCCAGAGTG TGCTCTGAAA ACATTGAGGT TTTTGGGTCC TGCAGCAGAA 
GAAGCCTGTC AGTTTGTGAC TGGAATTGTG GGTAAAAACC CGTTACTCCT GAAAGAACTG 
AATCTGAGTG ATCGTAAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GTAAACCCAA CACACTTCAG CTGAATAATA ACAGGATTAC AGCAGAAGGT 
TGTGCTGCTT TAACTTCAGC ATTTAATTTA AACCCTTCAC ACCTAATAGA GCTCAATCTG 
AGTGGGAATA AACTAAGAGA CTCAGGAGTA GAGGAGATCT GTCCTCTGCT GAAAAATACA 
CAGTGCAGAC TGGAGAGACT AAGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTAAAGGAG CTCACTGACT TACTGCAGGA TGAGCGATAT 
AGATTGAAGA CCCTCAGGCT TTTGAAAACT ACTGCAGCAC AAGAAGCGTG TGACTATCTT 
ACTAAAGTTG TGGGTAAAAG TCCATTATTA CTGACAGAAC TGGATCTGAG TGAAGATAAA 
TTAGGAGATC TGGATGGAGA GAAACTCTCT GCTCTTCTGA TGGACTCTCA TAGTAAAGTG 
GAGAAAATCA AGCTGAATAA TTGTAAGCAG ATTGAAAAAG GCTGTTCAGT ACTAGCTGCC 
GTTCTCTCCT CAAAAACAAT CCTAAAAGAG ATGAACCTGA ACAACAGTCG TCTGCTGGAC 
TCAGGAGTCT TAGAGATCTG TGAGGGACTG AAGAACCCTG TGTGTGAGCT GAAGATTCTC 
AAGCTCAGTA AGTGTGATTT AACAGAGGAA AGCTGTTCAG CTCTGGCTTC AGTTCTCAGA 
TCAGACTCCA GCAGTCTGAA GGATCTTGAC CTGAGCAATA ATAATCTGCA GGATTCAGGA 
GTGAAGTTGC TCTCTGATGG ACTAAAGCAC AGTAAACTGA AGAGACTCAG TCTTTCAGAC 
TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC 
ACTGACTTAC TGCAGGATCC ACACTGTACA CTAAAGACAC TGAGTTGTTT TAGTCCTGCT 
GCTGATGAAG GCTGTCAGTT TGTTACTGGA ATTGTGGGTA AAAACCCGTT ACTACTGAAA 
GAACTGAATC TGAGTGATCG TGAGCTAGGA GACACACGGG TGAATCAACT CTCTGCTCTA 
CTGCAAGATA AACACTGTAC ACTCAATATA CTCACGCTGA ATAATAACAG GATTACAGCA 
GAAGGTTGTG CTGCTTTGAC TTCAGCATTT AATTTAAACC CTTCACACCT GATAGAGCTC 
AATCTGAGTG GAAATAAACT AGGAGACTCA GGAGTAGAGA AGATCTGTCC TCTGCTGAAA 
AATACCCAGT GCAGATTGGA GAAATTAAAT CTTTCAGACT GCAGCATCAC TGGAAAAGGT 
TATGAAACAT TGATTTCAGC TTTGAAGTCA AACTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGGAAATC AGGAGTGCAG AAGCTCACTG ATTTGCTACG AGCTGGAAGC 
TGTAAATTAA AAACCATCAG GACTTTGAAA GGTTCCGCTG CACAGGAAGC GTGTGACTAT 
CTAACTAAAG CTGTGGGTAA AAGTCCATTA TTACTGACAG AACTGGATCT GAGTGAAGAT 
AAATTAGGAG ATCTGGATGG AGAGAAACTC TCTGCTCTTC TGATGGACTC TCATAGCAAA 
GTGGAGAAAA TCAGGATGAA TAACTGTAAA AAGCTGACAG AGAAAAGCTG TTTAGTTCTA 
GCTGCTGTTC TTTCATCCAA AACCATCTTG AAAGAGATGA ACCTAAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAAAGT GATCTGTGAG GGACTGAAGA ACTCTAAGCT GGAGATTTTA 
AAACTTTCAA ATTGTAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCTGGA 
GTAAAGGAGC TCACTGAGTT ACTGCAGAGT TCGTGGATTC CATGGAAAAA AGTGAAGTGT 
TCAATGAGGT TTTTGAGTCC TGCTGCTGAT GAATTTTGTC AGTTTGTGTC TGGAATTGTG 
GGTAAAAACC CGTTACTCCT GAAAGAACTG AATCTGAGTG ATCATGTACT AGCAGACACA 
CAAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT GCAAACTCAA CACACTCATT 
CTTTCAGACT GCAGTATCAG TGGAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACA ATCAGGAGTG 
AAAGAGCTCA CTGAGTTACT GCAGGATCCA CACTGTACAC TGAAGACACT GAGGTTTTTG 
AGTGATGATG CAGATGAAGC CTGTGAACAT TTGTATAAAG TTCTGGGTGG AAACCTGTTA 
CTCCTGAGAG AGCTGAATCT GACTAAACAT GATCTTGGAC CATCAGGATT AGAGAAACTT 
GCTGCAGTAC TGAAAGATAA ACACTGTAAA CTCAACACAC TGATTCTGAA TAACAGCACT 
ATCTCAGAGG AAGATTTTCA TGTTCTGACT GAAGCTTTTA ATTCAAACCC AACAAATCTG 
ACAGAGCTGA ACTTGAGTGG GACTAAACTC ACAAACTCAG GAATGAAGAG CTTCACAACT 
CTGTTTCAAA ACCAACAGTG TAGATTGGAA AAGCTGAAGT TCAGCAGCAT GTGTATTACA 
GCAGAAGGTT GTGCTGCTTT GACTTCAGCA TTTAACTCAA ACCCTTCACA CCTGAAAGAG 
CTCAATCTGA GTGGGAATAA ACTAGGAGAC TCAGGAGTAA GAAAAATCTC CAGTCTACTG 
AGAAACTCGC AGTGCATGCT GAAGATACTC GGTCTTTCAG ACTGCAGTAT CAGTGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT TACATCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACCATCAGGA GTGAAGGAGC TCACTGGCTT ACTACTCGAT 
CCACACTGTA CACTGACGAC ACTGAGGTTT TTGAGTCCTG CTGCTGATGA ATTCTGTCAG 
TTTGTGTCTG GAATTGTGGG TAAATACCCA TTACTCCTGA AAGAACTGAA TCTGAGTGAT 
CGTGGACTAG GGGACACACG AGTGAATCAA CTCTCTGCTC TACTGCAGGA TAAACACTTT 
AGACTCAACA CACTCATGCT GAATAATAAC AGGATTACAG CAGAAGGTTG TGCTGCTTTA 
ACTTCAGCAT TTAATTTAAA TCCTTCACAC CTGATAGAGC TCAATCTGAG TGGGAATAAA 
CTAGAAGACT CAGGAGTAGA GAAGATCTGT CCTCTGCTGG AAAATATACA ATGCCGATTA 
GAGAAACTGA GTCTTTCAGA CTGCAGTATC AGGGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGATTTA CTGCAGAGTA GAAGCTGTAA ATTCAGGTTT 
CTGAAGAGTT CTGCTGCACA GGAAGCATGC GAGTATCTTA CTAATGTTCT GGGTATAAGT 
CCATTATTAC TGACAGAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGACTGGGAG 
AAACTCTCTG CTCTTCTGAT GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTAAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGC TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AAAGATCTGT 
GAGGGACTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ATCTTTCAGA CTGCAGTATT 
ACAGAAGAAG GTTATGAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGAGTTA 
CTGCAGGATC CACACTGTGC ACTGAAGACA CTGAGATTTT TAAGAAGTCC TGCTGCTGAT 
GAAGGCTGTC AGTTAGTAAC TGGAGTTGTG GGTAAAAACC CGTTACTCCT GAAGGAACTG 
AATCTGAGTG ATCATGAGCT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GCCAGATCAA CACACTCAAT CTGAGTTATT GCAGTATTAC AGAGAAACAG 
TGTGAGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGGACCTC 
AGTGGGAATG AACTAGGAGA CTTTCAAAGC CTTGGCTCTC TACTGAAGAA CCAACAATTC 
AAGCTGGAGA AACTAATTCT GTGTGTTTGC AGTATTAAAG AGAAGCAGTG TGAGATCCTG 
ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGAACCTCAG TGGGAATGAA 
CTCAAGCACA GAAGAGTGAA GCAATTTTTT GATGTGCTGA AAAAATCAAC CTGTAAACTG 
GAGCGACTGA GGTTAAGATA CTGTAGTATA CAAGCCGATG ATTGTACTGA TCTGGCTTCA 
GCTCTGAAAT CAAACCCATC CCACCTGAGA GAACTGGACC TCAGTGGAAA CAATCTAGGA 
GACTCTGGAG TGAAAAAACT CAGTGATTTA CTGATAAACC AACAATTCAA GCTGGAGAAA 
CTACGTCTGA GTTATTGCAG TATTACAGAG AAACAGTGTG TGATCCTGAC TTCAGCTCTG 
AAATCAAACC CATCACACCT GAGAGAACTG AACCTCAATG GGAATAGACT AGGAGACTCT 
GGAGCTGATG GCCTTAACCA TCTGCTGAGC AGTGTGGATT GCAAGCTAAA TAAACTACAT 
CTGTGTGATT GCAGTATTAC AGAGAAACAG TTGCTCATCC CAATCACAGC TACGTGTATG 
GGCCTATTAC ACCTGAGAGA ACTGGACCTC AGTAAGAACC AAATAAAAAA CCAAGGAGTG 
TACATGCTGT GTGATGTGCT GAAAAACCCA CGCTGTAGAC TCAAGAGGTT AAGACTAAAT 
GACTGTGGCA TTACAGATGT TACACCCTTA ACTCAGTCTT TGACCAAGGA CAAAGCATTG 
AAGTTCTTAA AAGAGCTTGA ACTGAGTAAA AATCAAATGG GATTCTTCGA AAAGCAAAAA 
CTCAGTGACC TGCTGCGAGA CTCAAACTGT AAACTGAGGC TTGAGGAAGA AGAAGGTTAT 
TTTAGAGCTG GTGTAAAACG GGTTACTACA TTTTTGATGC CTCCATGGTC AAAAGTTCAA 
AAACCTGTGG ATCTCACAGG AGAGGAAAGT TCATCATCAG ATGAGGACGC CTCTGAAGAT 
GAGACTGATC AGGCAGAGGA GGCTGATAAG CTCTCAAATG AGGCGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_021469425.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325100.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X5, mRNA.

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CloneID	ODa151248
Clone ID Related Accession (Same CDS sequence)	XM_021469425.1
Accession Version	XM_021469425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein si:ch211-11p18.6 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACAACGGGGT GATTGCTGTA AATTAAAAAC CGTCAGGTTT 
TTAAAGAGTT CTGCTGCACA GGAAGCGTGT GACTATCTTA CTAAAGCTGT GGGTAAAAGT 
CCATTATTAC TGACAAAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAA GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTGAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAGATCTGT 
GAGGGGCTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ATCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGCTTTG 
ATTCCAGAGT GTGCTCTGAA AACATTGAGG TTTTTGGGTC CTGCAGCAGA AGAAGCCTGT 
CAGTTTGTGA CTGGAATTGT GGGTAAAAAC CCGTTACTCC TGAAAGAACT GAATCTGAGT 
GATCGTAAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGTAAACCCA ACACACTTCA GCTGAATAAT AACAGGATTA CAGCAGAAGG TTGTGCTGCT 
TTAACTTCAG CATTTAATTT AAACCCTTCA CACCTAATAG AGCTCAATCT GAGTGGGAAT 
AAACTAAGAG ACTCAGGAGT AGAGGAGATC TGTCCTCTGC TGAAAAATAC ACAGTGCAGA 
CTGGAGAGAC TAAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTAAAGGA GCTCACTGAC TTACTGCAGG ATGAGCGATA TAGATTGAAG 
ACCCTCAGGC TTTTGAAAAC TACTGCAGCA CAAGAAGCGT GTGACTATCT TACTAAAGTT 
GTGGGTAAAA GTCCATTATT ACTGACAGAA CTGGATCTGA GTGAAGATAA ATTAGGAGAT 
CTGGATGGAG AGAAACTCTC TGCTCTTCTG ATGGACTCTC ATAGTAAAGT GGAGAAAATC 
AAGCTGAATA ATTGTAAGCA GATTGAAAAA GGCTGTTCAG TACTAGCTGC CGTTCTCTCC 
TCAAAAACAA TCCTAAAAGA GATGAACCTG AACAACAGTC GTCTGCTGGA CTCAGGAGTC 
TTAGAGATCT GTGAGGGACT GAAGAACCCT GTGTGTGAGC TGAAGATTCT CAAGCTCAGT 
AAGTGTGATT TAACAGAGGA AAGCTGTTCA GCTCTGGCTT CAGTTCTCAG ATCAGACTCC 
AGCAGTCTGA AGGATCTTGA CCTGAGCAAT AATAATCTGC AGGATTCAGG AGTGAAGTTG 
CTCTCTGATG GACTAAAGCA CAGTAAACTG AAGAGACTCA GTCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGACTTA 
CTGCAGGATC CACACTGTAC ACTAAAGACA CTGAGTTGTT TTAGTCCTGC TGCTGATGAA 
GGCTGTCAGT TTGTTACTGG AATTGTGGGT AAAAACCCGT TACTACTGAA AGAACTGAAT 
CTGAGTGATC GTGAGCTAGG AGACACACGG GTGAATCAAC TCTCTGCTCT ACTGCAAGAT 
AAACACTGTA CACTCAATAT ACTCACGCTG AATAATAACA GGATTACAGC AGAAGGTTGT 
GCTGCTTTGA CTTCAGCATT TAATTTAAAC CCTTCACACC TGATAGAGCT CAATCTGAGT 
GGAAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGAA AAATACCCAG 
TGCAGATTGG AGAAATTAAA TCTTTCAGAC TGCAGCATCA CTGGAAAAGG TTATGAAACA 
TTGATTTCAG CTTTGAAGTC AAACTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGGAAAT CAGGAGTGCA GAAGCTCACT GATTTGCTAC GAGCTGGAAG CTGTAAATTA 
AAAACCATCA GGACTTTGAA AGGTTCCGCT GCACAGGAAG CGTGTGACTA TCTAACTAAA 
GCTGTGGGTA AAAGTCCATT ATTACTGACA GAACTGGATC TGAGTGAAGA TAAATTAGGA 
GATCTGGATG GAGAGAAACT CTCTGCTCTT CTGATGGACT CTCATAGCAA AGTGGAGAAA 
ATCAGGATGA ATAACTGTAA AAAGCTGACA GAGAAAAGCT GTTTAGTTCT AGCTGCTGTT 
CTTTCATCCA AAACCATCTT GAAAGAGATG AACCTAAACA ACAGTCGTCT GCTGGACTCA 
GGAGTCAAAG TGATCTGTGA GGGACTGAAG AACTCTAAGC TGGAGATTTT AAAACTTTCA 
AATTGTAGTA TTACAGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCTGG AGTAAAGGAG 
CTCACTGAGT TACTGCAGAG TTCGTGGATT CCATGGAAAA AAGTGAAGTG TTCAATGAGG 
TTTTTGAGTC CTGCTGCTGA TGAATTTTGT CAGTTTGTGT CTGGAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCATGTAC TAGCAGACAC ACAAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGCAAACTCA ACACACTCAT TCTTTCAGAC 
TGCAGTATCA GTGGAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAAGAGCTC 
ACTGAGTTAC TGCAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTGATGAT 
GCAGATGAAG CCTGTGAACA TTTGTATAAA GTTCTGGGTG GAAACCTGTT ACTCCTGAGA 
GAGCTGAATC TGACTAAACA TGATCTTGGA CCATCAGGAT TAGAGAAACT TGCTGCAGTA 
CTGAAAGATA AACACTGTAA ACTCAACACA CTGATTCTGA ATAACAGCAC TATCTCAGAG 
GAAGATTTTC ATGTTCTGAC TGAAGCTTTT AATTCAAACC CAACAAATCT GACAGAGCTG 
AACTTGAGTG GGACTAAACT CACAAACTCA GGAATGAAGA GCTTCACAAC TCTGTTTCAA 
AACCAACAGT GTAGATTGGA AAAGCTGAAG TTCAGCAGCA TGTGTATTAC AGCAGAAGGT 
TGTGCTGCTT TGACTTCAGC ATTTAACTCA AACCCTTCAC ACCTGAAAGA GCTCAATCTG 
AGTGGGAATA AACTAGGAGA CTCAGGAGTA AGAAAAATCT CCAGTCTACT GAGAAACTCG 
CAGTGCATGC TGAAGATACT CGGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TTACATCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACCATCAGG AGTGAAGGAG CTCACTGGCT TACTACTCGA TCCACACTGT 
ACACTGACGA CACTGAGGTT TTTGAGTCCT GCTGCTGATG AATTCTGTCA GTTTGTGTCT 
GGAATTGTGG GTAAATACCC ATTACTCCTG AAAGAACTGA ATCTGAGTGA TCGTGGACTA 
GGGGACACAC GAGTGAATCA ACTCTCTGCT CTACTGCAGG ATAAACACTT TAGACTCAAC 
ACACTCATGC TGAATAATAA CAGGATTACA GCAGAAGGTT GTGCTGCTTT AACTTCAGCA 
TTTAATTTAA ATCCTTCACA CCTGATAGAG CTCAATCTGA GTGGGAATAA ACTAGAAGAC 
TCAGGAGTAG AGAAGATCTG TCCTCTGCTG GAAAATATAC AATGCCGATT AGAGAAACTG 
AGTCTTTCAG ACTGCAGTAT CAGGGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGAGT AGAAGCTGTA AATTCAGGTT TCTGAAGAGT 
TCTGCTGCAC AGGAAGCATG CGAGTATCTT ACTAATGTTC TGGGTATAAG TCCATTATTA 
CTGACAGAAC TGGATCTGAG TGAAGTTAAA TTAGGAGATC TGGACTGGGA GAAACTCTCT 
GCTCTTCTGA TGGACTCTCA TAGCAAAGTG GAGAAAATCA AGCTAAATAA TTGTGAGCTG 
ACAGAGAAAA GCTGTTCAGT TCTAGCTGCT GTTCTCTCCT CCAAAACCAT CCTGAAAGAG 
CTGAACCTGA ACAACAGTCG TCTGCTGGAC TCAGGAGTCA GAAAGATCTG TGAGGGACTG 
AAGAATCCTG TGTGTGAGCT GAAGATACTC AATCTTTCAG ACTGCAGTAT TACAGAAGAA 
GGTTATGAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAGC TCACTGAGTT ACTGCAGGAT 
CCACACTGTG CACTGAAGAC ACTGAGATTT TTAAGAAGTC CTGCTGCTGA TGAAGGCTGT 
CAGTTAGTAA CTGGAGTTGT GGGTAAAAAC CCGTTACTCC TGAAGGAACT GAATCTGAGT 
GATCATGAGC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGCCAGATCA ACACACTCAA TCTGAGTTAT TGCAGTATTA CAGAGAAACA GTGTGAGATC 
CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGGACCT CAGTGGGAAT 
GAACTAGGAG ACTTTCAAAG CCTTGGCTCT CTACTGAAGA ACCAACAATT CAAGCTGGAG 
AAACTAATTC TGTGTGTTTG CAGTATTAAA GAGAAGCAGT GTGAGATCCT GACTTCAGCT 
CTGAAATCAA ACCCATCACA CCTGAGAGAA CTGAACCTCA GTGGGAATGA ACTCAAGCAC 
AGAAGAGTGA AGCAATTTTT TGATGTGCTG AAAAAATCAA CCTGTAAACT GGAGCGACTG 
AGGTTAAGAT ACTGTAGTAT ACAAGCCGAT GATTGTACTG ATCTGGCTTC AGCTCTGAAA 
TCAAACCCAT CCCACCTGAG AGAACTGGAC CTCAGTGGAA ACAATCTAGG AGACTCTGGA 
GTGAAAAAAC TCAGTGATTT ACTGATAAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG 
AGTTATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTGAT 
GGCCTTAACC ATCTGCTGAG CAGTGTGGAT TGCAAGCTAA ATAAACTACA TCTGTGTGAT 
TGCAGTATTA CAGAGAAACA GTTGCTCATC CCAATCACAG CTACGTGTAT GGGCCTATTA 
CACCTGAGAG AACTGGACCT CAGTAAGAAC CAAATAAAAA ACCAAGGAGT GTACATGCTG 
TGTGATGTGC TGAAAAACCC ACGCTGTAGA CTCAAGAGGT TAAGACTAAA TGACTGTGGC 
ATTACAGATG TTACACCCTT AACTCAGTCT TTGACCAAGG ACAAAGCATT GAAGTTCTTA 
AAAGAGCTTG AACTGAGTAA AAATCAAATG GGATTCTTCG AAAAGCAAAA ACTCAGTGAC 
CTGCTGCGAG ACTCAAACTG TAAACTGAGG CTTGAGGAAG AAGAAGGTTA TTTTAGAGCT 
GGTGTAAAAC GGGTTACTAC ATTTTTGATG CCTCCATGGT CAAAAGTTCA AAAACCTGTG 
GATCTCACAG GAGAGGAAAG TTCATCATCA GATGAGGACG CCTCTGAAGA TGAGACTGAT 
CAGGCAGAGG AGGCTGATAA GCTCTCAAAT GAGGCGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325100.1
CDS	65..8266
Translation	MSNNNLQDSGVKLLCTALKNVKCELEVLRISYCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGILGKNPLLLKELNLSDCELGDTGVNQLSALLQDKHCKPKTLMLRGCGLKEETCSALATVLGSDTSLKELDMSNNNLQDLGVKKLQSGLENTNCTLEKLSLSDCNISEEGYKALASALRSNPSHLIELNLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAAEEFCQFVTGIVGKNPLLLKELYLSDHELGDTRVNQLSALLQDKHCTVSTLILHNCGLTKESCSALASALSSDTSLKELDLCNNNLQDSGVKKLQSELQNTNWRLKKLRLSKCDLTEESCSALASVLTSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSKLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELIELLQDPLCTLKTLRFLSPAADEACQFVTGIVGKNLLLLKELNLSDRQLGDTRVNQLSALLQDKHCTLNTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACEHLYKVLGGNLLLLRELNLTKHDLGPSGLEKLAAVLKDKHCKLNTLILNNSTISEEDFHVLTEAFNSNPTNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_021469425.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469425.1

ORF Insert Sequence:

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACAACGGGGT GATTGCTGTA AATTAAAAAC CGTCAGGTTT 
TTAAAGAGTT CTGCTGCACA GGAAGCGTGT GACTATCTTA CTAAAGCTGT GGGTAAAAGT 
CCATTATTAC TGACAAAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAA GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTGAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAGATCTGT 
GAGGGGCTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ATCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGCTTTG 
ATTCCAGAGT GTGCTCTGAA AACATTGAGG TTTTTGGGTC CTGCAGCAGA AGAAGCCTGT 
CAGTTTGTGA CTGGAATTGT GGGTAAAAAC CCGTTACTCC TGAAAGAACT GAATCTGAGT 
GATCGTAAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGTAAACCCA ACACACTTCA GCTGAATAAT AACAGGATTA CAGCAGAAGG TTGTGCTGCT 
TTAACTTCAG CATTTAATTT AAACCCTTCA CACCTAATAG AGCTCAATCT GAGTGGGAAT 
AAACTAAGAG ACTCAGGAGT AGAGGAGATC TGTCCTCTGC TGAAAAATAC ACAGTGCAGA 
CTGGAGAGAC TAAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTAAAGGA GCTCACTGAC TTACTGCAGG ATGAGCGATA TAGATTGAAG 
ACCCTCAGGC TTTTGAAAAC TACTGCAGCA CAAGAAGCGT GTGACTATCT TACTAAAGTT 
GTGGGTAAAA GTCCATTATT ACTGACAGAA CTGGATCTGA GTGAAGATAA ATTAGGAGAT 
CTGGATGGAG AGAAACTCTC TGCTCTTCTG ATGGACTCTC ATAGTAAAGT GGAGAAAATC 
AAGCTGAATA ATTGTAAGCA GATTGAAAAA GGCTGTTCAG TACTAGCTGC CGTTCTCTCC 
TCAAAAACAA TCCTAAAAGA GATGAACCTG AACAACAGTC GTCTGCTGGA CTCAGGAGTC 
TTAGAGATCT GTGAGGGACT GAAGAACCCT GTGTGTGAGC TGAAGATTCT CAAGCTCAGT 
AAGTGTGATT TAACAGAGGA AAGCTGTTCA GCTCTGGCTT CAGTTCTCAG ATCAGACTCC 
AGCAGTCTGA AGGATCTTGA CCTGAGCAAT AATAATCTGC AGGATTCAGG AGTGAAGTTG 
CTCTCTGATG GACTAAAGCA CAGTAAACTG AAGAGACTCA GTCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGACTTA 
CTGCAGGATC CACACTGTAC ACTAAAGACA CTGAGTTGTT TTAGTCCTGC TGCTGATGAA 
GGCTGTCAGT TTGTTACTGG AATTGTGGGT AAAAACCCGT TACTACTGAA AGAACTGAAT 
CTGAGTGATC GTGAGCTAGG AGACACACGG GTGAATCAAC TCTCTGCTCT ACTGCAAGAT 
AAACACTGTA CACTCAATAT ACTCACGCTG AATAATAACA GGATTACAGC AGAAGGTTGT 
GCTGCTTTGA CTTCAGCATT TAATTTAAAC CCTTCACACC TGATAGAGCT CAATCTGAGT 
GGAAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGAA AAATACCCAG 
TGCAGATTGG AGAAATTAAA TCTTTCAGAC TGCAGCATCA CTGGAAAAGG TTATGAAACA 
TTGATTTCAG CTTTGAAGTC AAACTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGGAAAT CAGGAGTGCA GAAGCTCACT GATTTGCTAC GAGCTGGAAG CTGTAAATTA 
AAAACCATCA GGACTTTGAA AGGTTCCGCT GCACAGGAAG CGTGTGACTA TCTAACTAAA 
GCTGTGGGTA AAAGTCCATT ATTACTGACA GAACTGGATC TGAGTGAAGA TAAATTAGGA 
GATCTGGATG GAGAGAAACT CTCTGCTCTT CTGATGGACT CTCATAGCAA AGTGGAGAAA 
ATCAGGATGA ATAACTGTAA AAAGCTGACA GAGAAAAGCT GTTTAGTTCT AGCTGCTGTT 
CTTTCATCCA AAACCATCTT GAAAGAGATG AACCTAAACA ACAGTCGTCT GCTGGACTCA 
GGAGTCAAAG TGATCTGTGA GGGACTGAAG AACTCTAAGC TGGAGATTTT AAAACTTTCA 
AATTGTAGTA TTACAGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCTGG AGTAAAGGAG 
CTCACTGAGT TACTGCAGAG TTCGTGGATT CCATGGAAAA AAGTGAAGTG TTCAATGAGG 
TTTTTGAGTC CTGCTGCTGA TGAATTTTGT CAGTTTGTGT CTGGAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCATGTAC TAGCAGACAC ACAAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGCAAACTCA ACACACTCAT TCTTTCAGAC 
TGCAGTATCA GTGGAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAAGAGCTC 
ACTGAGTTAC TGCAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTGATGAT 
GCAGATGAAG CCTGTGAACA TTTGTATAAA GTTCTGGGTG GAAACCTGTT ACTCCTGAGA 
GAGCTGAATC TGACTAAACA TGATCTTGGA CCATCAGGAT TAGAGAAACT TGCTGCAGTA 
CTGAAAGATA AACACTGTAA ACTCAACACA CTGATTCTGA ATAACAGCAC TATCTCAGAG 
GAAGATTTTC ATGTTCTGAC TGAAGCTTTT AATTCAAACC CAACAAATCT GACAGAGCTG 
AACTTGAGTG GGACTAAACT CACAAACTCA GGAATGAAGA GCTTCACAAC TCTGTTTCAA 
AACCAACAGT GTAGATTGGA AAAGCTGAAG TTCAGCAGCA TGTGTATTAC AGCAGAAGGT 
TGTGCTGCTT TGACTTCAGC ATTTAACTCA AACCCTTCAC ACCTGAAAGA GCTCAATCTG 
AGTGGGAATA AACTAGGAGA CTCAGGAGTA AGAAAAATCT CCAGTCTACT GAGAAACTCG 
CAGTGCATGC TGAAGATACT CGGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TTACATCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACCATCAGG AGTGAAGGAG CTCACTGGCT TACTACTCGA TCCACACTGT 
ACACTGACGA CACTGAGGTT TTTGAGTCCT GCTGCTGATG AATTCTGTCA GTTTGTGTCT 
GGAATTGTGG GTAAATACCC ATTACTCCTG AAAGAACTGA ATCTGAGTGA TCGTGGACTA 
GGGGACACAC GAGTGAATCA ACTCTCTGCT CTACTGCAGG ATAAACACTT TAGACTCAAC 
ACACTCATGC TGAATAATAA CAGGATTACA GCAGAAGGTT GTGCTGCTTT AACTTCAGCA 
TTTAATTTAA ATCCTTCACA CCTGATAGAG CTCAATCTGA GTGGGAATAA ACTAGAAGAC 
TCAGGAGTAG AGAAGATCTG TCCTCTGCTG GAAAATATAC AATGCCGATT AGAGAAACTG 
AGTCTTTCAG ACTGCAGTAT CAGGGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGAGT AGAAGCTGTA AATTCAGGTT TCTGAAGAGT 
TCTGCTGCAC AGGAAGCATG CGAGTATCTT ACTAATGTTC TGGGTATAAG TCCATTATTA 
CTGACAGAAC TGGATCTGAG TGAAGTTAAA TTAGGAGATC TGGACTGGGA GAAACTCTCT 
GCTCTTCTGA TGGACTCTCA TAGCAAAGTG GAGAAAATCA AGCTAAATAA TTGTGAGCTG 
ACAGAGAAAA GCTGTTCAGT TCTAGCTGCT GTTCTCTCCT CCAAAACCAT CCTGAAAGAG 
CTGAACCTGA ACAACAGTCG TCTGCTGGAC TCAGGAGTCA GAAAGATCTG TGAGGGACTG 
AAGAATCCTG TGTGTGAGCT GAAGATACTC AATCTTTCAG ACTGCAGTAT TACAGAAGAA 
GGTTATGAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAGC TCACTGAGTT ACTGCAGGAT 
CCACACTGTG CACTGAAGAC ACTGAGATTT TTAAGAAGTC CTGCTGCTGA TGAAGGCTGT 
CAGTTAGTAA CTGGAGTTGT GGGTAAAAAC CCGTTACTCC TGAAGGAACT GAATCTGAGT 
GATCATGAGC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGCCAGATCA ACACACTCAA TCTGAGTTAT TGCAGTATTA CAGAGAAACA GTGTGAGATC 
CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGGACCT CAGTGGGAAT 
GAACTAGGAG ACTTTCAAAG CCTTGGCTCT CTACTGAAGA ACCAACAATT CAAGCTGGAG 
AAACTAATTC TGTGTGTTTG CAGTATTAAA GAGAAGCAGT GTGAGATCCT GACTTCAGCT 
CTGAAATCAA ACCCATCACA CCTGAGAGAA CTGAACCTCA GTGGGAATGA ACTCAAGCAC 
AGAAGAGTGA AGCAATTTTT TGATGTGCTG AAAAAATCAA CCTGTAAACT GGAGCGACTG 
AGGTTAAGAT ACTGTAGTAT ACAAGCCGAT GATTGTACTG ATCTGGCTTC AGCTCTGAAA 
TCAAACCCAT CCCACCTGAG AGAACTGGAC CTCAGTGGAA ACAATCTAGG AGACTCTGGA 
GTGAAAAAAC TCAGTGATTT ACTGATAAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG 
AGTTATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTGAT 
GGCCTTAACC ATCTGCTGAG CAGTGTGGAT TGCAAGCTAA ATAAACTACA TCTGTGTGAT 
TGCAGTATTA CAGAGAAACA GTTGCTCATC CCAATCACAG CTACGTGTAT GGGCCTATTA 
CACCTGAGAG AACTGGACCT CAGTAAGAAC CAAATAAAAA ACCAAGGAGT GTACATGCTG 
TGTGATGTGC TGAAAAACCC ACGCTGTAGA CTCAAGAGGT TAAGACTAAA TGACTGTGGC 
ATTACAGATG TTACACCCTT AACTCAGTCT TTGACCAAGG ACAAAGCATT GAAGTTCTTA 
AAAGAGCTTG AACTGAGTAA AAATCAAATG GGATTCTTCG AAAAGCAAAA ACTCAGTGAC 
CTGCTGCGAG ACTCAAACTG TAAACTGAGG CTTGAGGAAG AAGAAGGTTA TTTTAGAGCT 
GGTGTAAAAC GGGTTACTAC ATTTTTGATG CCTCCATGGT CAAAAGTTCA AAAACCTGTG 
GATCTCACAG GAGAGGAAAG TTCATCATCA GATGAGGACG CCTCTGAAGA TGAGACTGAT 
CAGGCAGAGG AGGCTGATAA GCTCTCAAAT GAGGCGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_021469426.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325101.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X6, mRNA.

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CloneID	ODa151249
Clone ID Related Accession (Same CDS sequence)	XM_021469426.1
Accession Version	XM_021469426.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein si:ch211-11p18.6 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACAACGGGGT GATTGCTGTA AATTAAAAAC CGTCAGGTTT 
TTAAAGAGTT CTGCTGCACA GGAAGCGTGT GACTATCTTA CTAAAGCTGT GGGTAAAAGT 
CCATTATTAC TGACAAAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAA GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTGAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAGATCTGT 
GAGGGGCTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ACCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACTTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGAAGCT CACTGACTTA 
CTGCAGGATC CACACTGTAC CCTGAATACA CTGAGGTTTT TGAGTCCTGC CGCAGAAGAA 
GCCTGTCAGT TTGTGACTGG AATTGTGGGT AAAAACCCGA TACTTCTGAA AGAACTGAAT 
CTGAGTGATC ATGAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AAAAACAGCT TTATCTCAGA GGAAGATTGT 
CGTGTTCTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGAGAGAGCT GAATTTGAAT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCAACA CTCTGTTTCA AAACCAACAG 
TGCAGACTGG AAAAGCTGAA GTTTAGCGGC ATCAGTATCA CAGCAGAAGG TTGTGCTGCT 
TTGACTTCAG CATTTAGCTC AAACCCGTCA CACCTGATAG AGCTAAATCT GAGTGGGAAT 
ACACTAGAAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC ACAGTGCATG 
CTGAAGATAC TCAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGCT TTGATTCCAG AGTGTGCTCT GAAAACATTG 
AGGTTTTTGG GTCCTGCAGC AGAAGAAGCC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCGTTAC TCCTGAAAGA ACTGAATCTG AGTGATCGTA AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC CCAACACACT TCAGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAA TTTAAACCCT 
TCACACCTAA TAGAGCTCAA TCTGAGTGGG AATAAACTAA GAGACTCAGG AGTAGAGGAG 
ATCTGTCCTC TGCTGAAAAA TACACAGTGC AGACTGGAGA GACTAAGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTAAA GGAGCTCACT 
GACTTACTGC AGGATGAGCG ATATAGATTG AAGACCCTCA GGCTTTTGAA AACTACTGCA 
GCACAAGAAG CGTGTGACTA TCTTACTAAA GTTGTGGGTA AAAGTCCATT ATTACTGACA 
GAACTGGATC TGAGTGAAGA TAAATTAGGA GATCTGGATG GAGAGAAACT CTCTGCTCTT 
CTGATGGACT CTCATAGTAA AGTGGAGAAA ATCAAGCTGA ATAATTGTAA GCAGATTGAA 
AAAGGCTGTT CAGTACTAGC TGCCGTTCTC TCCTCAAAAA CAATCCTAAA AGAGATGAAC 
CTGAACAACA GTCGTCTGCT GGACTCAGGA GTCTTAGAGA TCTGTGAGGG ACTGAAGAAC 
CCTGTGTGTG AGCTGAAGAT TCTCAAGCTC AGTAAGTGTG ATTTAACAGA GGAAAGCTGT 
TCAGCTCTGG CTTCAGTTCT CAGATCAGAC TCCAGCAGTC TGAAGGATCT TGACCTGAGC 
AATAATAATC TGCAGGATTC AGGAGTGAAG TTGCTCTCTG ATGGACTAAA GCACAGTAAA 
CTGAAGAGAC TCAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAC TTACTGCAGG ATCCACACTG TACACTAAAG 
ACACTGAGTT GTTTTAGTCC TGCTGCTGAT GAAGGCTGTC AGTTTGTTAC TGGAATTGTG 
GGTAAAAACC CGTTACTACT GAAAGAACTG AATCTGAGTG ATCGTGAGCT AGGAGACACA 
CGGGTGAATC AACTCTCTGC TCTACTGCAA GATAAACACT GTACACTCAA TATACTCACG 
CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAATTTA 
AACCCTTCAC ACCTGATAGA GCTCAATCTG AGTGGAAATA AACTAGGAGA CTCAGGAGTA 
GAGAAGATCT GTCCTCTGCT GAAAAATACC CAGTGCAGAT TGGAGAAATT AAATCTTTCA 
GACTGCAGCA TCACTGGAAA AGGTTATGAA ACATTGATTT CAGCTTTGAA GTCAAACTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGGA AATCAGGAGT GCAGAAGCTC 
ACTGATTTGC TACGAGCTGG AAGCTGTAAA TTAAAAACCA TCAGGACTTT GAAAGGTTCC 
GCTGCACAGG AAGCGTGTGA CTATCTAACT AAAGCTGTGG GTAAAAGTCC ATTATTACTG 
ACAGAACTGG ATCTGAGTGA AGATAAATTA GGAGATCTGG ATGGAGAGAA ACTCTCTGCT 
CTTCTGATGG ACTCTCATAG CAAAGTGGAG AAAATCAGGA TGAATAACTG TAAAAAGCTG 
ACAGAGAAAA GCTGTTTAGT TCTAGCTGCT GTTCTTTCAT CCAAAACCAT CTTGAAAGAG 
ATGAACCTAA ACAACAGTCG TCTGCTGGAC TCAGGAGTCA AAGTGATCTG TGAGGGACTG 
AAGAACTCTA AGCTGGAGAT TTTAAAACTT TCAAATTGTA GTATTACAGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC TGGAGTAAAG GAGCTCACTG AGTTACTGCA GAGTTCGTGG 
ATTCCATGGA AAAAAGTGAA GTGTTCAATG AGGTTTTTGA GTCCTGCTGC TGATGAATTT 
TGTCAGTTTG TGTCTGGAAT TGTGGGTAAA AACCCGTTAC TCCTGAAAGA ACTGAATCTG 
AGTGATCATG TACTAGCAGA CACACAAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA 
CACTGCAAAC TCAACACACT CATTCTTTCA GACTGCAGTA TCAGTGGAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAAGAG CTCACTGAGT TACTGCAGGA TCCACACTGT 
ACACTGAAGA CACTGAGGTT TTTGAGTCCT GCTGCTGATG AATTCTGTCA GTTTGTGTCT 
GGAATTGTGG GTAAATACCC ATTACTCCTG AAAGAACTGA ATCTGAGTGA TCGTGGACTA 
GGGGACACAC GAGTGAATCA ACTCTCTGCT CTACTGCAGG ATAAACACTT TAGACTCAAC 
ACACTCATGC TGAATAATAA CAGGATTACA GCAGAAGGTT GTGCTGCTTT AACTTCAGCA 
TTTAATTTAA ATCCTTCACA CCTGATAGAG CTCAATCTGA GTGGGAATAA ACTAGAAGAC 
TCAGGAGTAG AGAAGATCTG TCCTCTGCTG GAAAATATAC AATGCCGATT AGAGAAACTG 
AGTCTTTCAG ACTGCAGTAT CAGGGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGAGT AGAAGCTGTA AATTCAGGTT TCTGAAGAGT 
TCTGCTGCAC AGGAAGCATG CGAGTATCTT ACTAATGTTC TGGGTATAAG TCCATTATTA 
CTGACAGAAC TGGATCTGAG TGAAGTTAAA TTAGGAGATC TGGACTGGGA GAAACTCTCT 
GCTCTTCTGA TGGACTCTCA TAGCAAAGTG GAGAAAATCA AGCTAAATAA TTGTGAGCTG 
ACAGAGAAAA GCTGTTCAGT TCTAGCTGCT GTTCTCTCCT CCAAAACCAT CCTGAAAGAG 
CTGAACCTGA ACAACAGTCG TCTGCTGGAC TCAGGAGTCA GAAAGATCTG TGAGGGACTG 
AAGAATCCTG TGTGTGAGCT GAAGATACTC AATCTTTCAG ACTGCAGTAT TACAGAAGAA 
GGTTATGAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAGC TCACTGAGTT ACTGCAGGAT 
CCACACTGTG CACTGAAGAC ACTGAGATTT TTAAGAAGTC CTGCTGCTGA TGAAGGCTGT 
CAGTTAGTAA CTGGAGTTGT GGGTAAAAAC CCGTTACTCC TGAAGGAACT GAATCTGAGT 
GATCATGAGC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGCCAGATCA ACACACTCAA TCTGAGTTAT TGCAGTATTA CAGAGAAACA GTGTGAGATC 
CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGGACCT CAGTGGGAAT 
GAACTAGGAG ACTTTCAAAG CCTTGGCTCT CTACTGAAGA ACCAACAATT CAAGCTGGAG 
AAACTAATTC TGTGTGTTTG CAGTATTAAA GAGAAGCAGT GTGAGATCCT GACTTCAGCT 
CTGAAATCAA ACCCATCACA CCTGAGAGAA CTGAACCTCA GTGGGAATGA ACTCAAGCAC 
AGAAGAGTGA AGCAATTTTT TGATGTGCTG AAAAAATCAA CCTGTAAACT GGAGCGACTG 
AGGTTAAGAT ACTGTAGTAT ACAAGCCGAT GATTGTACTG ATCTGGCTTC AGCTCTGAAA 
TCAAACCCAT CCCACCTGAG AGAACTGGAC CTCAGTGGAA ACAATCTAGG AGACTCTGGA 
GTGAAAAAAC TCAGTGATTT ACTGATAAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG 
AGTTATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTGAT 
GGCCTTAACC ATCTGCTGAG CAGTGTGGAT TGCAAGCTAA ATAAACTACA TCTGTGTGAT 
TGCAGTATTA CAGAGAAACA GTTGCTCATC CCAATCACAG CTACGTGTAT GGGCCTATTA 
CACCTGAGAG AACTGGACCT CAGTAAGAAC CAAATAAAAA ACCAAGGAGT GTACATGCTG 
TGTGATGTGC TGAAAAACCC ACGCTGTAGA CTCAAGAGGT TAAGACTAAA TGACTGTGGC 
ATTACAGATG TTACACCCTT AACTCAGTCT TTGACCAAGG ACAAAGCATT GAAGTTCTTA 
AAAGAGCTTG AACTGAGTAA AAATCAAATG GGATTCTTCG AAAAGCAAAA ACTCAGTGAC 
CTGCTGCGAG ACTCAAACTG TAAACTGAGG CTTGAGGAAG AAGAAGGTTA TTTTAGAGCT 
GGTGTAAAAC GGGTTACTAC ATTTTTGATG CCTCCATGGT CAAAAGTTCA AAAACCTGTG 
GATCTCACAG GAGAGGAAAG TTCATCATCA GATGAGGACG CCTCTGAAGA TGAGACTGAT 
CAGGCAGAGG AGGCTGATAA GCTCTCAAAT GAGGCGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325101.1
CDS	66..8267
Translation	MSNNNLQDSGVKLLCTALKNVKCELEVLRISYCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGILGKNPLLLKELNLSDCELGDTGVNQLSALLQDKHCKPKTLMLRGCGLKEETCSALATVLGSDTSLKELDMSNNNLQDLGVKKLQSGLENTNCTLEKLSLSDCNISEEGYKALASALRSNPSHLIELNLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAAEEFCQFVTGIVGKNPLLLKELYLSDHELGDTRVNQLSALLQDKHCTVSTLILHNCGLTKESCSALASALSSDTSLKELDLCNNNLQDSGVKKLQSELQNTNWRLKKLRLSKCDLTEESCSALASVLTSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSKLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELIELLQDPLCTLKTLRFLSPAADEACQFVTGIVGKNLLLLKELNLSDRQLGDTRVNQLSALLQDKHCTLNTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLKFSGISITAEGCAALTSAFSSNPSHLIELNLSGNTLEDSGVTEISSLLANSQCMLKILSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_021469426.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469426.1

ORF Insert Sequence:

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACAACGGGGT GATTGCTGTA AATTAAAAAC CGTCAGGTTT 
TTAAAGAGTT CTGCTGCACA GGAAGCGTGT GACTATCTTA CTAAAGCTGT GGGTAAAAGT 
CCATTATTAC TGACAAAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAA GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTGAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAGATCTGT 
GAGGGGCTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ACCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACTTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGAAGCT CACTGACTTA 
CTGCAGGATC CACACTGTAC CCTGAATACA CTGAGGTTTT TGAGTCCTGC CGCAGAAGAA 
GCCTGTCAGT TTGTGACTGG AATTGTGGGT AAAAACCCGA TACTTCTGAA AGAACTGAAT 
CTGAGTGATC ATGAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AAAAACAGCT TTATCTCAGA GGAAGATTGT 
CGTGTTCTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGAGAGAGCT GAATTTGAAT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCAACA CTCTGTTTCA AAACCAACAG 
TGCAGACTGG AAAAGCTGAA GTTTAGCGGC ATCAGTATCA CAGCAGAAGG TTGTGCTGCT 
TTGACTTCAG CATTTAGCTC AAACCCGTCA CACCTGATAG AGCTAAATCT GAGTGGGAAT 
ACACTAGAAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC ACAGTGCATG 
CTGAAGATAC TCAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGCT TTGATTCCAG AGTGTGCTCT GAAAACATTG 
AGGTTTTTGG GTCCTGCAGC AGAAGAAGCC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCGTTAC TCCTGAAAGA ACTGAATCTG AGTGATCGTA AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC CCAACACACT TCAGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAA TTTAAACCCT 
TCACACCTAA TAGAGCTCAA TCTGAGTGGG AATAAACTAA GAGACTCAGG AGTAGAGGAG 
ATCTGTCCTC TGCTGAAAAA TACACAGTGC AGACTGGAGA GACTAAGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTAAA GGAGCTCACT 
GACTTACTGC AGGATGAGCG ATATAGATTG AAGACCCTCA GGCTTTTGAA AACTACTGCA 
GCACAAGAAG CGTGTGACTA TCTTACTAAA GTTGTGGGTA AAAGTCCATT ATTACTGACA 
GAACTGGATC TGAGTGAAGA TAAATTAGGA GATCTGGATG GAGAGAAACT CTCTGCTCTT 
CTGATGGACT CTCATAGTAA AGTGGAGAAA ATCAAGCTGA ATAATTGTAA GCAGATTGAA 
AAAGGCTGTT CAGTACTAGC TGCCGTTCTC TCCTCAAAAA CAATCCTAAA AGAGATGAAC 
CTGAACAACA GTCGTCTGCT GGACTCAGGA GTCTTAGAGA TCTGTGAGGG ACTGAAGAAC 
CCTGTGTGTG AGCTGAAGAT TCTCAAGCTC AGTAAGTGTG ATTTAACAGA GGAAAGCTGT 
TCAGCTCTGG CTTCAGTTCT CAGATCAGAC TCCAGCAGTC TGAAGGATCT TGACCTGAGC 
AATAATAATC TGCAGGATTC AGGAGTGAAG TTGCTCTCTG ATGGACTAAA GCACAGTAAA 
CTGAAGAGAC TCAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAC TTACTGCAGG ATCCACACTG TACACTAAAG 
ACACTGAGTT GTTTTAGTCC TGCTGCTGAT GAAGGCTGTC AGTTTGTTAC TGGAATTGTG 
GGTAAAAACC CGTTACTACT GAAAGAACTG AATCTGAGTG ATCGTGAGCT AGGAGACACA 
CGGGTGAATC AACTCTCTGC TCTACTGCAA GATAAACACT GTACACTCAA TATACTCACG 
CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAATTTA 
AACCCTTCAC ACCTGATAGA GCTCAATCTG AGTGGAAATA AACTAGGAGA CTCAGGAGTA 
GAGAAGATCT GTCCTCTGCT GAAAAATACC CAGTGCAGAT TGGAGAAATT AAATCTTTCA 
GACTGCAGCA TCACTGGAAA AGGTTATGAA ACATTGATTT CAGCTTTGAA GTCAAACTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGGA AATCAGGAGT GCAGAAGCTC 
ACTGATTTGC TACGAGCTGG AAGCTGTAAA TTAAAAACCA TCAGGACTTT GAAAGGTTCC 
GCTGCACAGG AAGCGTGTGA CTATCTAACT AAAGCTGTGG GTAAAAGTCC ATTATTACTG 
ACAGAACTGG ATCTGAGTGA AGATAAATTA GGAGATCTGG ATGGAGAGAA ACTCTCTGCT 
CTTCTGATGG ACTCTCATAG CAAAGTGGAG AAAATCAGGA TGAATAACTG TAAAAAGCTG 
ACAGAGAAAA GCTGTTTAGT TCTAGCTGCT GTTCTTTCAT CCAAAACCAT CTTGAAAGAG 
ATGAACCTAA ACAACAGTCG TCTGCTGGAC TCAGGAGTCA AAGTGATCTG TGAGGGACTG 
AAGAACTCTA AGCTGGAGAT TTTAAAACTT TCAAATTGTA GTATTACAGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC TGGAGTAAAG GAGCTCACTG AGTTACTGCA GAGTTCGTGG 
ATTCCATGGA AAAAAGTGAA GTGTTCAATG AGGTTTTTGA GTCCTGCTGC TGATGAATTT 
TGTCAGTTTG TGTCTGGAAT TGTGGGTAAA AACCCGTTAC TCCTGAAAGA ACTGAATCTG 
AGTGATCATG TACTAGCAGA CACACAAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA 
CACTGCAAAC TCAACACACT CATTCTTTCA GACTGCAGTA TCAGTGGAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAAGAG CTCACTGAGT TACTGCAGGA TCCACACTGT 
ACACTGAAGA CACTGAGGTT TTTGAGTCCT GCTGCTGATG AATTCTGTCA GTTTGTGTCT 
GGAATTGTGG GTAAATACCC ATTACTCCTG AAAGAACTGA ATCTGAGTGA TCGTGGACTA 
GGGGACACAC GAGTGAATCA ACTCTCTGCT CTACTGCAGG ATAAACACTT TAGACTCAAC 
ACACTCATGC TGAATAATAA CAGGATTACA GCAGAAGGTT GTGCTGCTTT AACTTCAGCA 
TTTAATTTAA ATCCTTCACA CCTGATAGAG CTCAATCTGA GTGGGAATAA ACTAGAAGAC 
TCAGGAGTAG AGAAGATCTG TCCTCTGCTG GAAAATATAC AATGCCGATT AGAGAAACTG 
AGTCTTTCAG ACTGCAGTAT CAGGGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGAGT AGAAGCTGTA AATTCAGGTT TCTGAAGAGT 
TCTGCTGCAC AGGAAGCATG CGAGTATCTT ACTAATGTTC TGGGTATAAG TCCATTATTA 
CTGACAGAAC TGGATCTGAG TGAAGTTAAA TTAGGAGATC TGGACTGGGA GAAACTCTCT 
GCTCTTCTGA TGGACTCTCA TAGCAAAGTG GAGAAAATCA AGCTAAATAA TTGTGAGCTG 
ACAGAGAAAA GCTGTTCAGT TCTAGCTGCT GTTCTCTCCT CCAAAACCAT CCTGAAAGAG 
CTGAACCTGA ACAACAGTCG TCTGCTGGAC TCAGGAGTCA GAAAGATCTG TGAGGGACTG 
AAGAATCCTG TGTGTGAGCT GAAGATACTC AATCTTTCAG ACTGCAGTAT TACAGAAGAA 
GGTTATGAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAGC TCACTGAGTT ACTGCAGGAT 
CCACACTGTG CACTGAAGAC ACTGAGATTT TTAAGAAGTC CTGCTGCTGA TGAAGGCTGT 
CAGTTAGTAA CTGGAGTTGT GGGTAAAAAC CCGTTACTCC TGAAGGAACT GAATCTGAGT 
GATCATGAGC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGCCAGATCA ACACACTCAA TCTGAGTTAT TGCAGTATTA CAGAGAAACA GTGTGAGATC 
CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGGACCT CAGTGGGAAT 
GAACTAGGAG ACTTTCAAAG CCTTGGCTCT CTACTGAAGA ACCAACAATT CAAGCTGGAG 
AAACTAATTC TGTGTGTTTG CAGTATTAAA GAGAAGCAGT GTGAGATCCT GACTTCAGCT 
CTGAAATCAA ACCCATCACA CCTGAGAGAA CTGAACCTCA GTGGGAATGA ACTCAAGCAC 
AGAAGAGTGA AGCAATTTTT TGATGTGCTG AAAAAATCAA CCTGTAAACT GGAGCGACTG 
AGGTTAAGAT ACTGTAGTAT ACAAGCCGAT GATTGTACTG ATCTGGCTTC AGCTCTGAAA 
TCAAACCCAT CCCACCTGAG AGAACTGGAC CTCAGTGGAA ACAATCTAGG AGACTCTGGA 
GTGAAAAAAC TCAGTGATTT ACTGATAAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG 
AGTTATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTGAT 
GGCCTTAACC ATCTGCTGAG CAGTGTGGAT TGCAAGCTAA ATAAACTACA TCTGTGTGAT 
TGCAGTATTA CAGAGAAACA GTTGCTCATC CCAATCACAG CTACGTGTAT GGGCCTATTA 
CACCTGAGAG AACTGGACCT CAGTAAGAAC CAAATAAAAA ACCAAGGAGT GTACATGCTG 
TGTGATGTGC TGAAAAACCC ACGCTGTAGA CTCAAGAGGT TAAGACTAAA TGACTGTGGC 
ATTACAGATG TTACACCCTT AACTCAGTCT TTGACCAAGG ACAAAGCATT GAAGTTCTTA 
AAAGAGCTTG AACTGAGTAA AAATCAAATG GGATTCTTCG AAAAGCAAAA ACTCAGTGAC 
CTGCTGCGAG ACTCAAACTG TAAACTGAGG CTTGAGGAAG AAGAAGGTTA TTTTAGAGCT 
GGTGTAAAAC GGGTTACTAC ATTTTTGATG CCTCCATGGT CAAAAGTTCA AAAACCTGTG 
GATCTCACAG GAGAGGAAAG TTCATCATCA GATGAGGACG CCTCTGAAGA TGAGACTGAT 
CAGGCAGAGG AGGCTGATAA GCTCTCAAAT GAGGCGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_021469427.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325102.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X7, mRNA.

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CloneID	ODa151250
Clone ID Related Accession (Same CDS sequence)	XM_021469427.1
Accession Version	XM_021469427.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein si:ch211-11p18.6 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACAACGGGGT GATTGCTGTA AATTAAAAAC CGTCAGGTTT 
TTAAAGAGTT CTGCTGCACA GGAAGCGTGT GACTATCTTA CTAAAGCTGT GGGTAAAAGT 
CCATTATTAC TGACAAAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAA GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTGAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAGATCTGT 
GAGGGGCTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ACCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACTTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGAAGCT CACTGACTTA 
CTGCAGGATC CACACTGTAC CCTGAATACA CTGAGGTTTT TGAGTCCTGC CGCAGAAGAA 
GCCTGTCAGT TTGTGACTGG AATTGTGGGT AAAAACCCGA TACTTCTGAA AGAACTGAAT 
CTGAGTGATC ATGAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AAAAACAGCT TTATCTCAGA GGAAGATTGT 
CGTGTTCTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGAGAGAGCT GAATTTGAAT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCAACA CTCTGTTTCA AAACCAACAG 
TGCAGACTGG AAAAGCTGAA GTTTAGCGGC ATCAGTATCA CAGCAGAAGG TTGTGCTGCT 
TTGACTTCAG CATTTAGCTC AAACCCGTCA CACCTGATAG AGCTAAATCT GAGTGGGAAT 
ACACTAGAAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC ACAGTGCATG 
CTGAAGATAC TCAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGCT TTGATTCCAG AGTGTGCTCT GAAAACATTG 
AGGTTTTTGG GTCCTGCAGC AGAAGAAGCC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCGTTAC TCCTGAAAGA ACTGAATCTG AGTGATCGTA AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC CCAACACACT TCAGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAA TTTAAACCCT 
TCACACCTAA TAGAGCTCAA TCTGAGTGGG AATAAACTAA GAGACTCAGG AGTAGAGGAG 
ATCTGTCCTC TGCTGAAAAA TACACAGTGC AGACTGGAGA GACTAAGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTAAA GGAGCTCACT 
GACTTACTGC AGGATGAGCG ATATAGATTG AAGACCCTCA GGCTTTTGAA AACTACTGCA 
GCACAAGAAG CGTGTGACTA TCTTACTAAA GTTGTGGGTA AAAGTCCATT ATTACTGACA 
GAACTGGATC TGAGTGAAGA TAAATTAGGA GATCTGGATG GAGAGAAACT CTCTGCTCTT 
CTGATGGACT CTCATAGTAA AGTGGAGAAA ATCAAGCTGA ATAATTGTAA GCAGATTGAA 
AAAGGCTGTT CAGTACTAGC TGCCGTTCTC TCCTCAAAAA CAATCCTAAA AGAGATGAAC 
CTGAACAACA GTCGTCTGCT GGACTCAGGA GTCTTAGAGA TCTGTGAGGG ACTGAAGAAC 
CCTGTGTGTG AGCTGAAGAT TCTCAAGCTC AGTAAGTGTG ATTTAACAGA GGAAAGCTGT 
TCAGCTCTGG CTTCAGTTCT CAGATCAGAC TCCAGCAGTC TGAAGGATCT TGACCTGAGC 
AATAATAATC TGCAGGATTC AGGAGTGAAG TTGCTCTCTG ATGGACTAAA GCACAGTAAA 
CTGAAGAGAC TCAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAC TTACTGCAGG ATCCACACTG TACACTAAAG 
ACACTGAGTT GTTTTAGTCC TGCTGCTGAT GAAGGCTGTC AGTTTGTTAC TGGAATTGTG 
GGTAAAAACC CGTTACTACT GAAAGAACTG AATCTGAGTG ATCGTGAGCT AGGAGACACA 
CGGGTGAATC AACTCTCTGC TCTACTGCAA GATAAACACT GTACACTCAA TATACTCACG 
CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAATTTA 
AACCCTTCAC ACCTGATAGA GCTCAATCTG AGTGGAAATA AACTAGGAGA CTCAGGAGTA 
GAGAAGATCT GTCCTCTGCT GAAAAATACC CAGTGCAGAT TGGAGAAATT AAATCTTTCA 
GACTGCAGCA TCACTGGAAA AGGTTATGAA ACATTGATTT CAGCTTTGAA GTCAAACTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGGA AATCAGGAGT GCAGAAGCTC 
ACTGATTTGC TACGAGCTGG AAGCTGTAAA TTAAAAACCA TCAGGACTTT GAAAGGTTCC 
GCTGCACAGG AAGCGTGTGA CTATCTAACT AAAGCTGTGG GTAAAAGTCC ATTATTACTG 
ACAGAACTGG ATCTGAGTGA AGATAAATTA GGAGATCTGG ATGGAGAGAA ACTCTCTGCT 
CTTCTGATGG ACTCTCATAG CAAAGTGGAG AAAATCAGGA TGAATAACTG TAAAAAGCTG 
ACAGAGAAAA GCTGTTTAGT TCTAGCTGCT GTTCTTTCAT CCAAAACCAT CTTGAAAGAG 
ATGAACCTAA ACAACAGTCG TCTGCTGGAC TCAGGAGTCA AAGTGATCTG TGAGGGACTG 
AAGAACTCTA AGCTGGAGAT TTTAAAACTT TCAAATTGTA GTATTACAGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC TGGAGTAAAG GAGCTCACTG AGTTACTGCA GAGTTCGTGG 
ATTCCATGGA AAAAAGTGAA GTGTTCAATG AGGTTTTTGA GTCCTGCTGC TGATGAATTT 
TGTCAGTTTG TGTCTGGAAT TGTGGGTAAA AACCCGTTAC TCCTGAAAGA ACTGAATCTG 
AGTGATCATG TACTAGCAGA CACACAAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA 
CACTGCAAAC TCAACACACT CATTCTTTCA GACTGCAGTA TCAGTGGAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAAGAG CTCACTGAGT TACTGCAGGA TCCACACTGT 
ACACTGAAGA CACTGAGGTT TTTGAGTGAT GATGCAGATG AAGCCTGTGA ACATTTGTAT 
AAAGTTCTGG GTGGAAACCT GTTACTCCTG AGAGAGCTGA ATCTGACTAA ACATGATCTT 
GGACCATCAG GATTAGAGAA ACTTGCTGCA GTACTGAAAG ATAAACACTG TAAACTCAAC 
ACACTGATTC TGAATAACAG CACTATCTCA GAGGAAGATT TTCATGTTCT GACTGAAGCT 
TTTAATTCAA ACCCAACAAA TCTGACAGAG CTGAACTTGA GTGGGACTAA ACTCACAAAC 
TCAGGAATGA AGAGCTTCAC AACTCTGTTT CAAAACCAAC AGTGTAGATT GGAAAAGCTG 
AAGTTCAGCA GCATGTGTAT TACAGCAGAA GGTTGTGCTG CTTTGACTTC AGCATTTAAC 
TCAAACCCTT CACACCTGAA AGAGCTCAAT CTGAGTGGGA ATAAACTAGG AGACTCAGGA 
GTAAGAAAAA TCTCCAGTCT ACTGAGAAAC TCGCAGTGCA TGCTGAAGAT ACTCGGTCTT 
TCAGACTGCA GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTTACATC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACCATC AGGAGTGAAG 
GAGCTCACTG GCTTACTACT CGATCCACAC TGTACACTGA CGACACTGAG GTTTTTGAGT 
CCTGCTGCTG ATGAATTCTG TCAGTTTGTG TCTGGAATTG TGGGTAAATA CCCATTACTC 
CTGAAAGAAC TGAATCTGAG TGATCGTGGA CTAGGGGACA CACGAGTGAA TCAACTCTCT 
GCTCTACTGC AGGATAAACA CTTTAGACTC AACACACTCA TGCTGAATAA TAACAGGATT 
ACAGCAGAAG GTTGTGCTGC TTTAACTTCA GCATTTAATT TAAATCCTTC ACACCTGATA 
GAGCTCAATC TGAGTGGGAA TAAACTAGAA GACTCAGGAG TAGAGAAGAT CTGTCCTCTG 
CTGGAAAATA TACAATGCCG ATTAGAGAAA CTGAGTCTTT CAGACTGCAG TATCAGGGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA TTTACTGCAG 
AGTAGAAGCT GTAAATTCAG GTTTCTGAAG AGTTCTGCTG CACAGGAAGC ATGCGAGTAT 
CTTACTAATG TTCTGGGTAT AAGTCCATTA TTACTGACAG AACTGGATCT GAGTGAAGTT 
AAATTAGGAG ATCTGGACTG GGAGAAACTC TCTGCTCTTC TGATGGACTC TCATAGCAAA 
GTGGAGAAAA TCAAGCTAAA TAATTGTGAG CTGACAGAGA AAAGCTGTTC AGTTCTAGCT 
GCTGTTCTCT CCTCCAAAAC CATCCTGAAA GAGCTGAACC TGAACAACAG TCGTCTGCTG 
GACTCAGGAG TCAGAAAGAT CTGTGAGGGA CTGAAGAATC CTGTGTGTGA GCTGAAGATA 
CTCAATCTTT CAGACTGCAG TATTACAGAA GAAGGTTATG AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA GTTACTGCAG GATCCACACT GTGCACTGAA GACACTGAGA 
TTTTTAAGAA GTCCTGCTGC TGATGAAGGC TGTCAGTTAG TAACTGGAGT TGTGGGTAAA 
AACCCGTTAC TCCTGAAGGA ACTGAATCTG AGTGATCATG AGCTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGCCAGA TCAACACACT CAATCTGAGT 
TATTGCAGTA TTACAGAGAA ACAGTGTGAG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGGA CCTCAGTGGG AATGAACTAG GAGACTTTCA AAGCCTTGGC 
TCTCTACTGA AGAACCAACA ATTCAAGCTG GAGAAACTAA TTCTGTGTGT TTGCAGTATT 
AAAGAGAAGC AGTGTGAGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA 
GAACTGAACC TCAGTGGGAA TGAACTCAAG CACAGAAGAG TGAAGCAATT TTTTGATGTG 
CTGAAAAAAT CAACCTGTAA ACTGGAGCGA CTGAGCTCTG AAATCAAACC CATCCCACCT 
GAGAGAACTG GACCTCAGTG GAAACAATCT AGGAGACTCT GGAGTGAAAA AACTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325102.1
CDS	68..8167
Translation	MSNNNLQDSGVKLLCTALKNVKCELEVLRISYCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGILGKNPLLLKELNLSDCELGDTGVNQLSALLQDKHCKPKTLMLRGCGLKEETCSALATVLGSDTSLKELDMSNNNLQDLGVKKLQSGLENTNCTLEKLSLSDCNISEEGYKALASALRSNPSHLIELNLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAAEEFCQFVTGIVGKNPLLLKELYLSDHELGDTRVNQLSALLQDKHCTVSTLILHNCGLTKESCSALASALSSDTSLKELDLCNNNLQDSGVKKLQSELQNTNWRLKKLRLSKCDLTEESCSALASVLTSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSKLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELIELLQDPLCTLKTLRFLSPAADEACQFVTGIVGKNLLLLKELNLSDRQLGDTRVNQLSALLQDKHCTLNTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLKFSGISITAEGCAALTSAFSSNPSHLIELNLSGNTLEDSGVTEISSLLANSQCMLKILSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACEHLYKVLGGNLLLLRELNLTKHDLGPSGLEKLAAVLKDKHCKLNTLILNNSTISEEDFHVLTEAFNSNPTNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLSSEIKPIPPERTGPQWKQSRRLWSEKTQ

Target ORF information:

RefSeq Version	XM_021469427.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469427.1

ORF Insert Sequence:

ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGC ACTGAAGAAC 
GTTAAGTGTG AATTAGAAGT ACTGAGAATC TCATACTGCA GTATCAGTGG AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCAGTTTGTG 
ACTGGAATTT TAGGTAAAAA CCCGTTACTC CTGAAAGAAC TCAATCTGAG TGATTGTGAA 
CTAGGAGACA CAGGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTAAACCT 
AAAACACTCA TGCTGCGTGG TTGTGGTCTA AAAGAGGAAA CTTGTTCAGC TCTGGCTACA 
GTTCTGGGAT CAGACACCAG TCTGAAAGAG CTTGACATGA GCAACAATAA TCTGCAGGAT 
TTAGGAGTGA AGAAACTCCA GAGCGGATTA GAAAATACAA ACTGCACACT GGAGAAACTC 
AGTCTTTCAG ACTGCAATAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGAAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CAGCAGAAGA ATTCTGTCAA TTTGTGACTG GAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGTA TCTGAGTGAT CATGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT ACAGTCAGCA CACTCATTCT GCATAATTGT 
GGTCTAACAA AGGAAAGCTG TTCAGCTCTG GCTTCAGCCT TGAGTTCAGA CACCAGTCTA 
AAAGAGCTCG ACCTGTGCAA CAATAATCTG CAGGATTCAG GAGTGAAGAA ACTCCAGAGC 
GAATTACAAA ATACAAACTG GAGACTGAAG AAACTCAGGC TCAGTAAGTG TGATCTAACA 
GAGGAAAGCT GTTCAGCTCT TGCTTCAGTT CTCACATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA AACTGGAGAA ACTCAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT AAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTGATAG AGTTACTGCA GGATCCACTC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGCCTG TCAGTTTGTG 
ACTGGAATTG TGGGTAAAAA CCTGTTACTC CTGAAAGAAC TGAATCTGAG TGATCGTCAA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTACTGC AGGATAAACA CTGTACACTC 
AACACACTCA TGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTGACTTCA 
GCATTTAATT TAAATCCATC AAACCTGATG GAGCTCACTC TGAGTGGAAA TAAACTAGAA 
GACTCAGGAG TAGAAAAGAT CTGTTCTCTG CTGAAAAATA CACAGTGCAG ATTGAAGAAA 
CTGAGTCTTT CAGACTGCAG GATCACTGAA GAAGGATATA AAAGTTTGAC TTTGTCCCTG 
AAGTCAAATT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACAACGGGGT GATTGCTGTA AATTAAAAAC CGTCAGGTTT 
TTAAAGAGTT CTGCTGCACA GGAAGCGTGT GACTATCTTA CTAAAGCTGT GGGTAAAAGT 
CCATTATTAC TGACAAAACT GGATCTGAGT GAAGTTAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAA GGACTCTCAT AGCAAAGTGG AGAAAATCAA GCTGAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT CTAGCTGCTG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAGATCTGT 
GAGGGGCTGA AGAATCCTGT GTGTGAGCTG AAGATACTCA ACCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACTTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGAAGCT CACTGACTTA 
CTGCAGGATC CACACTGTAC CCTGAATACA CTGAGGTTTT TGAGTCCTGC CGCAGAAGAA 
GCCTGTCAGT TTGTGACTGG AATTGTGGGT AAAAACCCGA TACTTCTGAA AGAACTGAAT 
CTGAGTGATC ATGAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AAAAACAGCT TTATCTCAGA GGAAGATTGT 
CGTGTTCTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGAGAGAGCT GAATTTGAAT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCAACA CTCTGTTTCA AAACCAACAG 
TGCAGACTGG AAAAGCTGAA GTTTAGCGGC ATCAGTATCA CAGCAGAAGG TTGTGCTGCT 
TTGACTTCAG CATTTAGCTC AAACCCGTCA CACCTGATAG AGCTAAATCT GAGTGGGAAT 
ACACTAGAAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC ACAGTGCATG 
CTGAAGATAC TCAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGCT TTGATTCCAG AGTGTGCTCT GAAAACATTG 
AGGTTTTTGG GTCCTGCAGC AGAAGAAGCC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCGTTAC TCCTGAAAGA ACTGAATCTG AGTGATCGTA AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC CCAACACACT TCAGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAA TTTAAACCCT 
TCACACCTAA TAGAGCTCAA TCTGAGTGGG AATAAACTAA GAGACTCAGG AGTAGAGGAG 
ATCTGTCCTC TGCTGAAAAA TACACAGTGC AGACTGGAGA GACTAAGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTAAA GGAGCTCACT 
GACTTACTGC AGGATGAGCG ATATAGATTG AAGACCCTCA GGCTTTTGAA AACTACTGCA 
GCACAAGAAG CGTGTGACTA TCTTACTAAA GTTGTGGGTA AAAGTCCATT ATTACTGACA 
GAACTGGATC TGAGTGAAGA TAAATTAGGA GATCTGGATG GAGAGAAACT CTCTGCTCTT 
CTGATGGACT CTCATAGTAA AGTGGAGAAA ATCAAGCTGA ATAATTGTAA GCAGATTGAA 
AAAGGCTGTT CAGTACTAGC TGCCGTTCTC TCCTCAAAAA CAATCCTAAA AGAGATGAAC 
CTGAACAACA GTCGTCTGCT GGACTCAGGA GTCTTAGAGA TCTGTGAGGG ACTGAAGAAC 
CCTGTGTGTG AGCTGAAGAT TCTCAAGCTC AGTAAGTGTG ATTTAACAGA GGAAAGCTGT 
TCAGCTCTGG CTTCAGTTCT CAGATCAGAC TCCAGCAGTC TGAAGGATCT TGACCTGAGC 
AATAATAATC TGCAGGATTC AGGAGTGAAG TTGCTCTCTG ATGGACTAAA GCACAGTAAA 
CTGAAGAGAC TCAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAC TTACTGCAGG ATCCACACTG TACACTAAAG 
ACACTGAGTT GTTTTAGTCC TGCTGCTGAT GAAGGCTGTC AGTTTGTTAC TGGAATTGTG 
GGTAAAAACC CGTTACTACT GAAAGAACTG AATCTGAGTG ATCGTGAGCT AGGAGACACA 
CGGGTGAATC AACTCTCTGC TCTACTGCAA GATAAACACT GTACACTCAA TATACTCACG 
CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAATTTA 
AACCCTTCAC ACCTGATAGA GCTCAATCTG AGTGGAAATA AACTAGGAGA CTCAGGAGTA 
GAGAAGATCT GTCCTCTGCT GAAAAATACC CAGTGCAGAT TGGAGAAATT AAATCTTTCA 
GACTGCAGCA TCACTGGAAA AGGTTATGAA ACATTGATTT CAGCTTTGAA GTCAAACTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGGA AATCAGGAGT GCAGAAGCTC 
ACTGATTTGC TACGAGCTGG AAGCTGTAAA TTAAAAACCA TCAGGACTTT GAAAGGTTCC 
GCTGCACAGG AAGCGTGTGA CTATCTAACT AAAGCTGTGG GTAAAAGTCC ATTATTACTG 
ACAGAACTGG ATCTGAGTGA AGATAAATTA GGAGATCTGG ATGGAGAGAA ACTCTCTGCT 
CTTCTGATGG ACTCTCATAG CAAAGTGGAG AAAATCAGGA TGAATAACTG TAAAAAGCTG 
ACAGAGAAAA GCTGTTTAGT TCTAGCTGCT GTTCTTTCAT CCAAAACCAT CTTGAAAGAG 
ATGAACCTAA ACAACAGTCG TCTGCTGGAC TCAGGAGTCA AAGTGATCTG TGAGGGACTG 
AAGAACTCTA AGCTGGAGAT TTTAAAACTT TCAAATTGTA GTATTACAGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC TGGAGTAAAG GAGCTCACTG AGTTACTGCA GAGTTCGTGG 
ATTCCATGGA AAAAAGTGAA GTGTTCAATG AGGTTTTTGA GTCCTGCTGC TGATGAATTT 
TGTCAGTTTG TGTCTGGAAT TGTGGGTAAA AACCCGTTAC TCCTGAAAGA ACTGAATCTG 
AGTGATCATG TACTAGCAGA CACACAAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA 
CACTGCAAAC TCAACACACT CATTCTTTCA GACTGCAGTA TCAGTGGAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAAGAG CTCACTGAGT TACTGCAGGA TCCACACTGT 
ACACTGAAGA CACTGAGGTT TTTGAGTGAT GATGCAGATG AAGCCTGTGA ACATTTGTAT 
AAAGTTCTGG GTGGAAACCT GTTACTCCTG AGAGAGCTGA ATCTGACTAA ACATGATCTT 
GGACCATCAG GATTAGAGAA ACTTGCTGCA GTACTGAAAG ATAAACACTG TAAACTCAAC 
ACACTGATTC TGAATAACAG CACTATCTCA GAGGAAGATT TTCATGTTCT GACTGAAGCT 
TTTAATTCAA ACCCAACAAA TCTGACAGAG CTGAACTTGA GTGGGACTAA ACTCACAAAC 
TCAGGAATGA AGAGCTTCAC AACTCTGTTT CAAAACCAAC AGTGTAGATT GGAAAAGCTG 
AAGTTCAGCA GCATGTGTAT TACAGCAGAA GGTTGTGCTG CTTTGACTTC AGCATTTAAC 
TCAAACCCTT CACACCTGAA AGAGCTCAAT CTGAGTGGGA ATAAACTAGG AGACTCAGGA 
GTAAGAAAAA TCTCCAGTCT ACTGAGAAAC TCGCAGTGCA TGCTGAAGAT ACTCGGTCTT 
TCAGACTGCA GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTTACATC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACCATC AGGAGTGAAG 
GAGCTCACTG GCTTACTACT CGATCCACAC TGTACACTGA CGACACTGAG GTTTTTGAGT 
CCTGCTGCTG ATGAATTCTG TCAGTTTGTG TCTGGAATTG TGGGTAAATA CCCATTACTC 
CTGAAAGAAC TGAATCTGAG TGATCGTGGA CTAGGGGACA CACGAGTGAA TCAACTCTCT 
GCTCTACTGC AGGATAAACA CTTTAGACTC AACACACTCA TGCTGAATAA TAACAGGATT 
ACAGCAGAAG GTTGTGCTGC TTTAACTTCA GCATTTAATT TAAATCCTTC ACACCTGATA 
GAGCTCAATC TGAGTGGGAA TAAACTAGAA GACTCAGGAG TAGAGAAGAT CTGTCCTCTG 
CTGGAAAATA TACAATGCCG ATTAGAGAAA CTGAGTCTTT CAGACTGCAG TATCAGGGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA TTTACTGCAG 
AGTAGAAGCT GTAAATTCAG GTTTCTGAAG AGTTCTGCTG CACAGGAAGC ATGCGAGTAT 
CTTACTAATG TTCTGGGTAT AAGTCCATTA TTACTGACAG AACTGGATCT GAGTGAAGTT 
AAATTAGGAG ATCTGGACTG GGAGAAACTC TCTGCTCTTC TGATGGACTC TCATAGCAAA 
GTGGAGAAAA TCAAGCTAAA TAATTGTGAG CTGACAGAGA AAAGCTGTTC AGTTCTAGCT 
GCTGTTCTCT CCTCCAAAAC CATCCTGAAA GAGCTGAACC TGAACAACAG TCGTCTGCTG 
GACTCAGGAG TCAGAAAGAT CTGTGAGGGA CTGAAGAATC CTGTGTGTGA GCTGAAGATA 
CTCAATCTTT CAGACTGCAG TATTACAGAA GAAGGTTATG AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA GTTACTGCAG GATCCACACT GTGCACTGAA GACACTGAGA 
TTTTTAAGAA GTCCTGCTGC TGATGAAGGC TGTCAGTTAG TAACTGGAGT TGTGGGTAAA 
AACCCGTTAC TCCTGAAGGA ACTGAATCTG AGTGATCATG AGCTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGCCAGA TCAACACACT CAATCTGAGT 
TATTGCAGTA TTACAGAGAA ACAGTGTGAG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGGA CCTCAGTGGG AATGAACTAG GAGACTTTCA AAGCCTTGGC 
TCTCTACTGA AGAACCAACA ATTCAAGCTG GAGAAACTAA TTCTGTGTGT TTGCAGTATT 
AAAGAGAAGC AGTGTGAGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA 
GAACTGAACC TCAGTGGGAA TGAACTCAAG CACAGAAGAG TGAAGCAATT TTTTGATGTG 
CTGAAAAAAT CAACCTGTAA ACTGGAGCGA CTGAGCTCTG AAATCAAACC CATCCCACCT 
GAGAGAACTG GACCTCAGTG GAAACAATCT AGGAGACTCT GGAGTGAAAA AACTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_017353260.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208749.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X1, mRNA.

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CloneID	ODa57131
Clone ID Related Accession (Same CDS sequence)	XM_017353260.1
Accession Version	XM_017353260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein si:ch211-11p18.6 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAACGGGGTG ATTGCTGTAA ATTAAAAACC GTCAGGTTTT TAAAGAGTTC TGCTGCACAG 
GAAGCGTGTG ACTATCTTAC TAAAGCTGTG GGTAAAAGTC CATTATTACT GACAAAACTG 
GATCTGAGTG AAGTTAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGAAG 
GACTCTCATA GCAAAGTGGA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCAGTTC TAGCTGCTGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAGATCTGTG AGGGGCTGAA GAATCCTGTG 
TGTGAGCTGA AGATACTCAA CCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACTTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGACTTAC TGCAGGATCC ACACTGTACC 
CTGAATACAC TGAGGTTTTT GAGTCCTGCC GCAGAAGAAG CCTGTCAGTT TGTGACTGGA 
ATTGTGGGTA AAAACCCGAT ACTTCTGAAA GAACTGAATC TGAGTGATCA TGAACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGTAA ACTCAACACA 
CTGATTCTGA AAAACAGCTT TATCTCAGAG GAAGATTGTC GTGTTCTGGC TGAAGCTTTT 
AATTCAAACC CATCAAATCT GAGAGAGCTG AATTTGAATG GGACTAAACT CACAAACTCA 
GGAATGAAGA GCTTCAACAC TCTGTTTCAA AACCAACAGT GCAGACTGGA AAAGCTGAAG 
TTTAGCGGCA TCAGTATCAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAGCTCA 
AACCCGTCAC ACCTGATAGA GCTAAATCTG AGTGGGAATA CACTAGAAGA CTCAGGAGTA 
ACAGAAATCT CCAGTCTACT GGCAAACTCA CAGTGCATGC TGAAGATACT CAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGCTT TGATTCCAGA GTGTGCTCTG AAAACATTGA GGTTTTTGGG TCCTGCAGCA 
GAAGAAGCCT GTCAGTTTGT GACTGGAATT GTGGGTAAAA ACCCGTTACT CCTGAAAGAA 
CTGAATCTGA GTGATCGTAA ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTACTG 
CAGGATAAAC ACTGTAAACC CAACACACTT CAGCTGAATA ATAACAGGAT TACAGCAGAA 
GGTTGTGCTG CTTTAACTTC AGCATTTAAT TTAAACCCTT CACACCTAAT AGAGCTCAAT 
CTGAGTGGGA ATAAACTAAG AGACTCAGGA GTAGAGGAGA TCTGTCCTCT GCTGAAAAAT 
ACACAGTGCA GACTGGAGAG ACTAAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTAAAG GAGCTCACTG ACTTACTGCA GGATGAGCGA 
TATAGATTGA AGACCCTCAG GCTTTTGAAA ACTACTGCAG CACAAGAAGC GTGTGACTAT 
CTTACTAAAG TTGTGGGTAA AAGTCCATTA TTACTGACAG AACTGGATCT GAGTGAAGAT 
AAATTAGGAG ATCTGGATGG AGAGAAACTC TCTGCTCTTC TGATGGACTC TCATAGTAAA 
GTGGAGAAAA TCAAGCTGAA TAATTGTAAG CAGATTGAAA AAGGCTGTTC AGTACTAGCT 
GCCGTTCTCT CCTCAAAAAC AATCCTAAAA GAGATGAACC TGAACAACAG TCGTCTGCTG 
GACTCAGGAG TCTTAGAGAT CTGTGAGGGA CTGAAGAACC CTGTGTGTGA GCTGAAGATT 
CTCAAGCTCA GTAAGTGTGA TTTAACAGAG GAAAGCTGTT CAGCTCTGGC TTCAGTTCTC 
AGATCAGACT CCAGCAGTCT GAAGGATCTT GACCTGAGCA ATAATAATCT GCAGGATTCA 
GGAGTGAAGT TGCTCTCTGA TGGACTAAAG CACAGTAAAC TGAAGAGACT CAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGACT TACTGCAGGA TCCACACTGT ACACTAAAGA CACTGAGTTG TTTTAGTCCT 
GCTGCTGATG AAGGCTGTCA GTTTGTTACT GGAATTGTGG GTAAAAACCC GTTACTACTG 
AAAGAACTGA ATCTGAGTGA TCGTGAGCTA GGAGACACAC GGGTGAATCA ACTCTCTGCT 
CTACTGCAAG ATAAACACTG TACACTCAAT ATACTCACGC TGAATAATAA CAGGATTACA 
GCAGAAGGTT GTGCTGCTTT GACTTCAGCA TTTAATTTAA ACCCTTCACA CCTGATAGAG 
CTCAATCTGA GTGGAAATAA ACTAGGAGAC TCAGGAGTAG AGAAGATCTG TCCTCTGCTG 
AAAAATACCC AGTGCAGATT GGAGAAATTA AATCTTTCAG ACTGCAGCAT CACTGGAAAA 
GGTTATGAAA CATTGATTTC AGCTTTGAAG TCAAACTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGGAA ATCAGGAGTG CAGAAGCTCA CTGATTTGCT ACGAGCTGGA 
AGCTGTAAAT TAAAAACCAT CAGGACTTTG AAAGGTTCCG CTGCACAGGA AGCGTGTGAC 
TATCTAACTA AAGCTGTGGG TAAAAGTCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA CTCTCTGCTC TTCTGATGGA CTCTCATAGC 
AAAGTGGAGA AAATCAGGAT GAATAACTGT AAAAAGCTGA CAGAGAAAAG CTGTTTAGTT 
CTAGCTGCTG TTCTTTCATC CAAAACCATC TTGAAAGAGA TGAACCTAAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAA AGTGATCTGT GAGGGACTGA AGAACTCTAA GCTGGAGATT 
TTAAAACTTT CAAATTGTAG TATTACAGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCT 
GGAGTAAAGG AGCTCACTGA GTTACTGCAG AGTTCGTGGA TTCCATGGAA AAAAGTGAAG 
TGTTCAATGA GGTTTTTGAG TCCTGCTGCT GATGAATTTT GTCAGTTTGT GTCTGGAATT 
GTGGGTAAAA ACCCGTTACT CCTGAAAGAA CTGAATCTGA GTGATCATGT ACTAGCAGAC 
ACACAAGTGA ATCAACTCTC TGCTCTACTG CAGGATAAAC ACTGCAAACT CAACACACTC 
ATTCTTTCAG ACTGCAGTAT CAGTGGAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAAGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTGATG ATGCAGATGA AGCCTGTGAA CATTTGTATA AAGTTCTGGG TGGAAACCTG 
TTACTCCTGA GAGAGCTGAA TCTGACTAAA CATGATCTTG GACCATCAGG ATTAGAGAAA 
CTTGCTGCAG TACTGAAAGA TAAACACTGT AAACTCAACA CACTGATTCT GAATAACAGC 
ACTATCTCAG AGGAAGATTT TCATGTTCTG ACTGAAGCTT TTAATTCAAA CCCAACAAAT 
CTGACAGAGC TGAACTTGAG TGGGACTAAA CTCACAAACT CAGGAATGAA GAGCTTCACA 
ACTCTGTTTC AAAACCAACA GTGTAGATTG GAAAAGCTGA AGTTCAGCAG CATGTGTATT 
ACAGCAGAAG GTTGTGCTGC TTTGACTTCA GCATTTAACT CAAACCCTTC ACACCTGAAA 
GAGCTCAATC TGAGTGGGAA TAAACTAGGA GACTCAGGAG TAAGAAAAAT CTCCAGTCTA 
CTGAGAAACT CGCAGTGCAT GCTGAAGATA CTCGGTCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTTACATCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACCATCA GGAGTGAAGG AGCTCACTGG CTTACTACTC 
GATCCACACT GTACACTGAC GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGAATTCTGT 
CAGTTTGTGT CTGGAATTGT GGGTAAATAC CCATTACTCC TGAAAGAACT GAATCTGAGT 
GATCGTGGAC TAGGGGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TTTAGACTCA ACACACTCAT GCTGAATAAT AACAGGATTA CAGCAGAAGG TTGTGCTGCT 
TTAACTTCAG CATTTAATTT AAATCCTTCA CACCTGATAG AGCTCAATCT GAGTGGGAAT 
AAACTAGAAG ACTCAGGAGT AGAGAAGATC TGTCCTCTGC TGGAAAATAT ACAATGCCGA 
TTAGAGAAAC TGAGTCTTTC AGACTGCAGT ATCAGGGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAT TTACTGCAGA GTAGAAGCTG TAAATTCAGG 
TTTCTGAAGA GTTCTGCTGC ACAGGAAGCA TGCGAGTATC TTACTAATGT TCTGGGTATA 
AGTCCATTAT TACTGACAGA ACTGGATCTG AGTGAAGTTA AATTAGGAGA TCTGGACTGG 
GAGAAACTCT CTGCTCTTCT GATGGACTCT CATAGCAAAG TGGAGAAAAT CAAGCTAAAT 
AATTGTGAGC TGACAGAGAA AAGCTGTTCA GTTCTAGCTG CTGTTCTCTC CTCCAAAACC 
ATCCTGAAAG AGCTGAACCT GAACAACAGT CGTCTGCTGG ACTCAGGAGT CAGAAAGATC 
TGTGAGGGAC TGAAGAATCC TGTGTGTGAG CTGAAGATAC TCAATCTTTC AGACTGCAGT 
ATTACAGAAG AAGGTTATGA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAG 
TTACTGCAGG ATCCACACTG TGCACTGAAG ACACTGAGAT TTTTAAGAAG TCCTGCTGCT 
GATGAAGGCT GTCAGTTAGT AACTGGAGTT GTGGGTAAAA ACCCGTTACT CCTGAAGGAA 
CTGAATCTGA GTGATCATGA GCTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTACTG 
CAGGATAAAC ACTGCCAGAT CAACACACTC AATCTGAGTT ATTGCAGTAT TACAGAGAAA 
CAGTGTGAGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGGAC 
CTCAGTGGGA ATGAACTAGG AGACTTTCAA AGCCTTGGCT CTCTACTGAA GAACCAACAA 
TTCAAGCTGG AGAAACTAAT TCTGTGTGTT TGCAGTATTA AAGAGAAGCA GTGTGAGATC 
CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGAACCT CAGTGGGAAT 
GAACTCAAGC ACAGAAGAGT GAAGCAATTT TTTGATGTGC TGAAAAAATC AACCTGTAAA 
CTGGAGCGAC TGAGGTTAAG ATACTGTAGT ATACAAGCCG ATGATTGTAC TGATCTGGCT 
TCAGCTCTGA AATCAAACCC ATCCCACCTG AGAGAACTGG ACCTCAGTGG AAACAATCTA 
GGAGACTCTG GAGTGAAAAA ACTCAGTGAT TTACTGATAA ACCAACAATT CAAGCTGGAG 
AAACTACGTC TGAGTTATTG CAGTATTACA GAGAAACAGT GTGTGATCCT GACTTCAGCT 
CTGAAATCAA ACCCATCACA CCTGAGAGAA CTGAACCTCA ATGGGAATAG ACTAGGAGAC 
TCTGGAGCTG ATGGCCTTAA CCATCTGCTG AGCAGTGTGG ATTGCAAGCT AAATAAACTA 
CATCTGTGTG ATTGCAGTAT TACAGAGAAA CAGTTGCTCA TCCCAATCAC AGCTACGTGT 
ATGGGCCTAT TACACCTGAG AGAACTGGAC CTCAGTAAGA ACCAAATAAA AAACCAAGGA 
GTGTACATGC TGTGTGATGT GCTGAAAAAC CCACGCTGTA GACTCAAGAG GTTAAGACTA 
AATGACTGTG GCATTACAGA TGTTACACCC TTAACTCAGT CTTTGACCAA GGACAAAGCA 
TTGAAGTTCT TAAAAGAGCT TGAACTGAGT AAAAATCAAA TGGGATTCTT CGAAAAGCAA 
AAACTCAGTG ACCTGCTGCG AGACTCAAAC TGTAAACTGA GGCTTGAGGA AGAAGAAGGT 
TATTTTAGAG CTGGTGTAAA ACGGGTTACT ACATTTTTGA TGCCTCCATG GTCAAAAGTT 
CAAAAACCTG TGGATCTCAC AGGAGAGGAA AGTTCATCAT CAGATGAGGA CGCCTCTGAA 
GATGAGACTG ATCAGGCAGA GGAGGCTGAT AAGCTCTCAA ATGAGGCGGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208749.1
CDS	188..10201
Translation	MKMSLSEKHSVRSGSHVSSSVSVKSDRSKGHMPDFSETKTSPNKRFQYKTLEFDFQPQRSCNNFKDLQCIFQGLESKLFNFLKKELENFKKILQKENTQYLVKDFNENRCSIREAILDLTLYFLREMKQDEAADTLEDELIFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVASRRHEAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKKNREISFIFPLPFREMNLKEKETLSLMDLITQFFPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCCDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIERLTEIRGFNDAQKEEYFRKRLTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILQEKRNNGVKNNQADDASKTLQKSNTEDTPKTLTQMYTHFLRFQIQQSRRKYDGENTPDVSWDKDAILSLGKLAFHQLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVVGTMYCFVHLSVQEFIAALYAHLILDINKKSVFDQDSKNRNESMIDLLRTAVDKTLESDNGHLDLFLRFLLGLSLQSNRRLLQGLLTQQEHNDQSKEEIVQYIKQKFEVNHSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSADLSPAQWSALAFVLLTSEEEMEEFELQKFKKSDECLIRLSAVIKTCKRALLNDCNLTDRSCSTVAAVLRSENSLKELNMSNNNLQDSGVKLLCAGLKNIKCDLETLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSAAADEGCQFVTGIVGKNPLLLKELSLSGHELGDTRVNQLSALLQDKHCQINTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLKFSGISITAEGCAALTSAFSSNPSHLIELNLSGNTLEDSGVTEISSLLANSQCMLKILSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACEHLYKVLGGNLLLLRELNLTKHDLGPSGLEKLAAVLKDKHCKLNTLILNNSTISEEDFHVLTEAFNSNPTNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_017353260.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353260.1

ORF Insert Sequence:

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAACGGGGTG ATTGCTGTAA ATTAAAAACC GTCAGGTTTT TAAAGAGTTC TGCTGCACAG 
GAAGCGTGTG ACTATCTTAC TAAAGCTGTG GGTAAAAGTC CATTATTACT GACAAAACTG 
GATCTGAGTG AAGTTAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGAAG 
GACTCTCATA GCAAAGTGGA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCAGTTC TAGCTGCTGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAGATCTGTG AGGGGCTGAA GAATCCTGTG 
TGTGAGCTGA AGATACTCAA CCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACTTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGACTTAC TGCAGGATCC ACACTGTACC 
CTGAATACAC TGAGGTTTTT GAGTCCTGCC GCAGAAGAAG CCTGTCAGTT TGTGACTGGA 
ATTGTGGGTA AAAACCCGAT ACTTCTGAAA GAACTGAATC TGAGTGATCA TGAACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGTAA ACTCAACACA 
CTGATTCTGA AAAACAGCTT TATCTCAGAG GAAGATTGTC GTGTTCTGGC TGAAGCTTTT 
AATTCAAACC CATCAAATCT GAGAGAGCTG AATTTGAATG GGACTAAACT CACAAACTCA 
GGAATGAAGA GCTTCAACAC TCTGTTTCAA AACCAACAGT GCAGACTGGA AAAGCTGAAG 
TTTAGCGGCA TCAGTATCAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAGCTCA 
AACCCGTCAC ACCTGATAGA GCTAAATCTG AGTGGGAATA CACTAGAAGA CTCAGGAGTA 
ACAGAAATCT CCAGTCTACT GGCAAACTCA CAGTGCATGC TGAAGATACT CAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGCTT TGATTCCAGA GTGTGCTCTG AAAACATTGA GGTTTTTGGG TCCTGCAGCA 
GAAGAAGCCT GTCAGTTTGT GACTGGAATT GTGGGTAAAA ACCCGTTACT CCTGAAAGAA 
CTGAATCTGA GTGATCGTAA ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTACTG 
CAGGATAAAC ACTGTAAACC CAACACACTT CAGCTGAATA ATAACAGGAT TACAGCAGAA 
GGTTGTGCTG CTTTAACTTC AGCATTTAAT TTAAACCCTT CACACCTAAT AGAGCTCAAT 
CTGAGTGGGA ATAAACTAAG AGACTCAGGA GTAGAGGAGA TCTGTCCTCT GCTGAAAAAT 
ACACAGTGCA GACTGGAGAG ACTAAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTAAAG GAGCTCACTG ACTTACTGCA GGATGAGCGA 
TATAGATTGA AGACCCTCAG GCTTTTGAAA ACTACTGCAG CACAAGAAGC GTGTGACTAT 
CTTACTAAAG TTGTGGGTAA AAGTCCATTA TTACTGACAG AACTGGATCT GAGTGAAGAT 
AAATTAGGAG ATCTGGATGG AGAGAAACTC TCTGCTCTTC TGATGGACTC TCATAGTAAA 
GTGGAGAAAA TCAAGCTGAA TAATTGTAAG CAGATTGAAA AAGGCTGTTC AGTACTAGCT 
GCCGTTCTCT CCTCAAAAAC AATCCTAAAA GAGATGAACC TGAACAACAG TCGTCTGCTG 
GACTCAGGAG TCTTAGAGAT CTGTGAGGGA CTGAAGAACC CTGTGTGTGA GCTGAAGATT 
CTCAAGCTCA GTAAGTGTGA TTTAACAGAG GAAAGCTGTT CAGCTCTGGC TTCAGTTCTC 
AGATCAGACT CCAGCAGTCT GAAGGATCTT GACCTGAGCA ATAATAATCT GCAGGATTCA 
GGAGTGAAGT TGCTCTCTGA TGGACTAAAG CACAGTAAAC TGAAGAGACT CAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGACT TACTGCAGGA TCCACACTGT ACACTAAAGA CACTGAGTTG TTTTAGTCCT 
GCTGCTGATG AAGGCTGTCA GTTTGTTACT GGAATTGTGG GTAAAAACCC GTTACTACTG 
AAAGAACTGA ATCTGAGTGA TCGTGAGCTA GGAGACACAC GGGTGAATCA ACTCTCTGCT 
CTACTGCAAG ATAAACACTG TACACTCAAT ATACTCACGC TGAATAATAA CAGGATTACA 
GCAGAAGGTT GTGCTGCTTT GACTTCAGCA TTTAATTTAA ACCCTTCACA CCTGATAGAG 
CTCAATCTGA GTGGAAATAA ACTAGGAGAC TCAGGAGTAG AGAAGATCTG TCCTCTGCTG 
AAAAATACCC AGTGCAGATT GGAGAAATTA AATCTTTCAG ACTGCAGCAT CACTGGAAAA 
GGTTATGAAA CATTGATTTC AGCTTTGAAG TCAAACTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGGAA ATCAGGAGTG CAGAAGCTCA CTGATTTGCT ACGAGCTGGA 
AGCTGTAAAT TAAAAACCAT CAGGACTTTG AAAGGTTCCG CTGCACAGGA AGCGTGTGAC 
TATCTAACTA AAGCTGTGGG TAAAAGTCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA CTCTCTGCTC TTCTGATGGA CTCTCATAGC 
AAAGTGGAGA AAATCAGGAT GAATAACTGT AAAAAGCTGA CAGAGAAAAG CTGTTTAGTT 
CTAGCTGCTG TTCTTTCATC CAAAACCATC TTGAAAGAGA TGAACCTAAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAA AGTGATCTGT GAGGGACTGA AGAACTCTAA GCTGGAGATT 
TTAAAACTTT CAAATTGTAG TATTACAGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCT 
GGAGTAAAGG AGCTCACTGA GTTACTGCAG AGTTCGTGGA TTCCATGGAA AAAAGTGAAG 
TGTTCAATGA GGTTTTTGAG TCCTGCTGCT GATGAATTTT GTCAGTTTGT GTCTGGAATT 
GTGGGTAAAA ACCCGTTACT CCTGAAAGAA CTGAATCTGA GTGATCATGT ACTAGCAGAC 
ACACAAGTGA ATCAACTCTC TGCTCTACTG CAGGATAAAC ACTGCAAACT CAACACACTC 
ATTCTTTCAG ACTGCAGTAT CAGTGGAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAAGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTGATG ATGCAGATGA AGCCTGTGAA CATTTGTATA AAGTTCTGGG TGGAAACCTG 
TTACTCCTGA GAGAGCTGAA TCTGACTAAA CATGATCTTG GACCATCAGG ATTAGAGAAA 
CTTGCTGCAG TACTGAAAGA TAAACACTGT AAACTCAACA CACTGATTCT GAATAACAGC 
ACTATCTCAG AGGAAGATTT TCATGTTCTG ACTGAAGCTT TTAATTCAAA CCCAACAAAT 
CTGACAGAGC TGAACTTGAG TGGGACTAAA CTCACAAACT CAGGAATGAA GAGCTTCACA 
ACTCTGTTTC AAAACCAACA GTGTAGATTG GAAAAGCTGA AGTTCAGCAG CATGTGTATT 
ACAGCAGAAG GTTGTGCTGC TTTGACTTCA GCATTTAACT CAAACCCTTC ACACCTGAAA 
GAGCTCAATC TGAGTGGGAA TAAACTAGGA GACTCAGGAG TAAGAAAAAT CTCCAGTCTA 
CTGAGAAACT CGCAGTGCAT GCTGAAGATA CTCGGTCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTTACATCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACCATCA GGAGTGAAGG AGCTCACTGG CTTACTACTC 
GATCCACACT GTACACTGAC GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGAATTCTGT 
CAGTTTGTGT CTGGAATTGT GGGTAAATAC CCATTACTCC TGAAAGAACT GAATCTGAGT 
GATCGTGGAC TAGGGGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TTTAGACTCA ACACACTCAT GCTGAATAAT AACAGGATTA CAGCAGAAGG TTGTGCTGCT 
TTAACTTCAG CATTTAATTT AAATCCTTCA CACCTGATAG AGCTCAATCT GAGTGGGAAT 
AAACTAGAAG ACTCAGGAGT AGAGAAGATC TGTCCTCTGC TGGAAAATAT ACAATGCCGA 
TTAGAGAAAC TGAGTCTTTC AGACTGCAGT ATCAGGGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAT TTACTGCAGA GTAGAAGCTG TAAATTCAGG 
TTTCTGAAGA GTTCTGCTGC ACAGGAAGCA TGCGAGTATC TTACTAATGT TCTGGGTATA 
AGTCCATTAT TACTGACAGA ACTGGATCTG AGTGAAGTTA AATTAGGAGA TCTGGACTGG 
GAGAAACTCT CTGCTCTTCT GATGGACTCT CATAGCAAAG TGGAGAAAAT CAAGCTAAAT 
AATTGTGAGC TGACAGAGAA AAGCTGTTCA GTTCTAGCTG CTGTTCTCTC CTCCAAAACC 
ATCCTGAAAG AGCTGAACCT GAACAACAGT CGTCTGCTGG ACTCAGGAGT CAGAAAGATC 
TGTGAGGGAC TGAAGAATCC TGTGTGTGAG CTGAAGATAC TCAATCTTTC AGACTGCAGT 
ATTACAGAAG AAGGTTATGA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAG 
TTACTGCAGG ATCCACACTG TGCACTGAAG ACACTGAGAT TTTTAAGAAG TCCTGCTGCT 
GATGAAGGCT GTCAGTTAGT AACTGGAGTT GTGGGTAAAA ACCCGTTACT CCTGAAGGAA 
CTGAATCTGA GTGATCATGA GCTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTACTG 
CAGGATAAAC ACTGCCAGAT CAACACACTC AATCTGAGTT ATTGCAGTAT TACAGAGAAA 
CAGTGTGAGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGGAC 
CTCAGTGGGA ATGAACTAGG AGACTTTCAA AGCCTTGGCT CTCTACTGAA GAACCAACAA 
TTCAAGCTGG AGAAACTAAT TCTGTGTGTT TGCAGTATTA AAGAGAAGCA GTGTGAGATC 
CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGAACCT CAGTGGGAAT 
GAACTCAAGC ACAGAAGAGT GAAGCAATTT TTTGATGTGC TGAAAAAATC AACCTGTAAA 
CTGGAGCGAC TGAGGTTAAG ATACTGTAGT ATACAAGCCG ATGATTGTAC TGATCTGGCT 
TCAGCTCTGA AATCAAACCC ATCCCACCTG AGAGAACTGG ACCTCAGTGG AAACAATCTA 
GGAGACTCTG GAGTGAAAAA ACTCAGTGAT TTACTGATAA ACCAACAATT CAAGCTGGAG 
AAACTACGTC TGAGTTATTG CAGTATTACA GAGAAACAGT GTGTGATCCT GACTTCAGCT 
CTGAAATCAA ACCCATCACA CCTGAGAGAA CTGAACCTCA ATGGGAATAG ACTAGGAGAC 
TCTGGAGCTG ATGGCCTTAA CCATCTGCTG AGCAGTGTGG ATTGCAAGCT AAATAAACTA 
CATCTGTGTG ATTGCAGTAT TACAGAGAAA CAGTTGCTCA TCCCAATCAC AGCTACGTGT 
ATGGGCCTAT TACACCTGAG AGAACTGGAC CTCAGTAAGA ACCAAATAAA AAACCAAGGA 
GTGTACATGC TGTGTGATGT GCTGAAAAAC CCACGCTGTA GACTCAAGAG GTTAAGACTA 
AATGACTGTG GCATTACAGA TGTTACACCC TTAACTCAGT CTTTGACCAA GGACAAAGCA 
TTGAAGTTCT TAAAAGAGCT TGAACTGAGT AAAAATCAAA TGGGATTCTT CGAAAAGCAA 
AAACTCAGTG ACCTGCTGCG AGACTCAAAC TGTAAACTGA GGCTTGAGGA AGAAGAAGGT 
TATTTTAGAG CTGGTGTAAA ACGGGTTACT ACATTTTTGA TGCCTCCATG GTCAAAAGTT 
CAAAAACCTG TGGATCTCAC AGGAGAGGAA AGTTCATCAT CAGATGAGGA CGCCTCTGAA 
GATGAGACTG ATCAGGCAGA GGAGGCTGAT AAGCTCTCAA ATGAGGCGGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_017353261.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208750.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X2, mRNA.

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CloneID	ODa57132
Clone ID Related Accession (Same CDS sequence)	XM_017353261.1
Accession Version	XM_017353261.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein si:ch211-11p18.6 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAACGGGGTG ATTGCTGTAA ATTAAAAACC GTCAGGTTTT TAAAGAGTTC TGCTGCACAG 
GAAGCGTGTG ACTATCTTAC TAAAGCTGTG GGTAAAAGTC CATTATTACT GACAAAACTG 
GATCTGAGTG AAGTTAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGAAG 
GACTCTCATA GCAAAGTGGA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCAGTTC TAGCTGCTGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAGATCTGTG AGGGGCTGAA GAATCCTGTG 
TGTGAGCTGA AGATACTCAA TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGGAGCTC ACTGCTTTGA TTCCAGAGTG TGCTCTGAAA 
ACATTGAGGT TTTTGGGTCC TGCAGCAGAA GAAGCCTGTC AGTTTGTGAC TGGAATTGTG 
GGTAAAAACC CGTTACTCCT GAAAGAACTG AATCTGAGTG ATCGTAAACT AGGAGACACA 
CGAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT GTAAACCCAA CACACTTCAG 
CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TAACTTCAGC ATTTAATTTA 
AACCCTTCAC ACCTAATAGA GCTCAATCTG AGTGGGAATA AACTAAGAGA CTCAGGAGTA 
GAGGAGATCT GTCCTCTGCT GAAAAATACA CAGTGCAGAC TGGAGAGACT AAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTAAAGGAG 
CTCACTGACT TACTGCAGGA TGAGCGATAT AGATTGAAGA CCCTCAGGCT TTTGAAAACT 
ACTGCAGCAC AAGAAGCGTG TGACTATCTT ACTAAAGTTG TGGGTAAAAG TCCATTATTA 
CTGACAGAAC TGGATCTGAG TGAAGATAAA TTAGGAGATC TGGATGGAGA GAAACTCTCT 
GCTCTTCTGA TGGACTCTCA TAGTAAAGTG GAGAAAATCA AGCTGAATAA TTGTAAGCAG 
ATTGAAAAAG GCTGTTCAGT ACTAGCTGCC GTTCTCTCCT CAAAAACAAT CCTAAAAGAG 
ATGAACCTGA ACAACAGTCG TCTGCTGGAC TCAGGAGTCT TAGAGATCTG TGAGGGACTG 
AAGAACCCTG TGTGTGAGCT GAAGATTCTC AAGCTCAGTA AGTGTGATTT AACAGAGGAA 
AGCTGTTCAG CTCTGGCTTC AGTTCTCAGA TCAGACTCCA GCAGTCTGAA GGATCTTGAC 
CTGAGCAATA ATAATCTGCA GGATTCAGGA GTGAAGTTGC TCTCTGATGG ACTAAAGCAC 
AGTAAACTGA AGAGACTCAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGGAGCTC ACTGACTTAC TGCAGGATCC ACACTGTACA 
CTAAAGACAC TGAGTTGTTT TAGTCCTGCT GCTGATGAAG GCTGTCAGTT TGTTACTGGA 
ATTGTGGGTA AAAACCCGTT ACTACTGAAA GAACTGAATC TGAGTGATCG TGAGCTAGGA 
GACACACGGG TGAATCAACT CTCTGCTCTA CTGCAAGATA AACACTGTAC ACTCAATATA 
CTCACGCTGA ATAATAACAG GATTACAGCA GAAGGTTGTG CTGCTTTGAC TTCAGCATTT 
AATTTAAACC CTTCACACCT GATAGAGCTC AATCTGAGTG GAAATAAACT AGGAGACTCA 
GGAGTAGAGA AGATCTGTCC TCTGCTGAAA AATACCCAGT GCAGATTGGA GAAATTAAAT 
CTTTCAGACT GCAGCATCAC TGGAAAAGGT TATGAAACAT TGATTTCAGC TTTGAAGTCA 
AACTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGGAAATC AGGAGTGCAG 
AAGCTCACTG ATTTGCTACG AGCTGGAAGC TGTAAATTAA AAACCATCAG GACTTTGAAA 
GGTTCCGCTG CACAGGAAGC GTGTGACTAT CTAACTAAAG CTGTGGGTAA AAGTCCATTA 
TTACTGACAG AACTGGATCT GAGTGAAGAT AAATTAGGAG ATCTGGATGG AGAGAAACTC 
TCTGCTCTTC TGATGGACTC TCATAGCAAA GTGGAGAAAA TCAGGATGAA TAACTGTAAA 
AAGCTGACAG AGAAAAGCTG TTTAGTTCTA GCTGCTGTTC TTTCATCCAA AACCATCTTG 
AAAGAGATGA ACCTAAACAA CAGTCGTCTG CTGGACTCAG GAGTCAAAGT GATCTGTGAG 
GGACTGAAGA ACTCTAAGCT GGAGATTTTA AAACTTTCAA ATTGTAGTAT TACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCTGGA GTAAAGGAGC TCACTGAGTT ACTGCAGAGT 
TCGTGGATTC CATGGAAAAA AGTGAAGTGT TCAATGAGGT TTTTGAGTCC TGCTGCTGAT 
GAATTTTGTC AGTTTGTGTC TGGAATTGTG GGTAAAAACC CGTTACTCCT GAAAGAACTG 
AATCTGAGTG ATCATGTACT AGCAGACACA CAAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GCAAACTCAA CACACTCATT CTTTCAGACT GCAGTATCAG TGGAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGAGATCA AACCCTTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAAGAGCTCA CTGAGTTACT GCAGGATCCA 
CACTGTACAC TGAAGACACT GAGGTTTTTG AGTGATGATG CAGATGAAGC CTGTGAACAT 
TTGTATAAAG TTCTGGGTGG AAACCTGTTA CTCCTGAGAG AGCTGAATCT GACTAAACAT 
GATCTTGGAC CATCAGGATT AGAGAAACTT GCTGCAGTAC TGAAAGATAA ACACTGTAAA 
CTCAACACAC TGATTCTGAA TAACAGCACT ATCTCAGAGG AAGATTTTCA TGTTCTGACT 
GAAGCTTTTA ATTCAAACCC AACAAATCTG ACAGAGCTGA ACTTGAGTGG GACTAAACTC 
ACAAACTCAG GAATGAAGAG CTTCACAACT CTGTTTCAAA ACCAACAGTG TAGATTGGAA 
AAGCTGAAGT TCAGCAGCAT GTGTATTACA GCAGAAGGTT GTGCTGCTTT GACTTCAGCA 
TTTAACTCAA ACCCTTCACA CCTGAAAGAG CTCAATCTGA GTGGGAATAA ACTAGGAGAC 
TCAGGAGTAA GAAAAATCTC CAGTCTACTG AGAAACTCGC AGTGCATGCT GAAGATACTC 
GGTCTTTCAG ACTGCAGTAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT TACATCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACCATCAGGA 
GTGAAGGAGC TCACTGGCTT ACTACTCGAT CCACACTGTA CACTGACGAC ACTGAGGTTT 
TTGAGTCCTG CTGCTGATGA ATTCTGTCAG TTTGTGTCTG GAATTGTGGG TAAATACCCA 
TTACTCCTGA AAGAACTGAA TCTGAGTGAT CGTGGACTAG GGGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTTT AGACTCAACA CACTCATGCT GAATAATAAC 
AGGATTACAG CAGAAGGTTG TGCTGCTTTA ACTTCAGCAT TTAATTTAAA TCCTTCACAC 
CTGATAGAGC TCAATCTGAG TGGGAATAAA CTAGAAGACT CAGGAGTAGA GAAGATCTGT 
CCTCTGCTGG AAAATATACA ATGCCGATTA GAGAAACTGA GTCTTTCAGA CTGCAGTATC 
AGGGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CTGCAGAGTA GAAGCTGTAA ATTCAGGTTT CTGAAGAGTT CTGCTGCACA GGAAGCATGC 
GAGTATCTTA CTAATGTTCT GGGTATAAGT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGTTAAAT TAGGAGATCT GGACTGGGAG AAACTCTCTG CTCTTCTGAT GGACTCTCAT 
AGCAAAGTGG AGAAAATCAA GCTAAATAAT TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT 
CTAGCTGCTG TTCTCTCCTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AAAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA ATCTTTCAGA CTGCAGTATT ACAGAAGAAG GTTATGAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTGC ACTGAAGACA 
CTGAGATTTT TAAGAAGTCC TGCTGCTGAT GAAGGCTGTC AGTTAGTAAC TGGAGTTGTG 
GGTAAAAACC CGTTACTCCT GAAGGAACTG AATCTGAGTG ATCATGAGCT AGGAGACACA 
CGAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT GCCAGATCAA CACACTCAAT 
CTGAGTTATT GCAGTATTAC AGAGAAACAG TGTGAGATCC TGACTTCAGC TCTGAAATCA 
AACCCATCAC ACCTGAGAGA ACTGGACCTC AGTGGGAATG AACTAGGAGA CTTTCAAAGC 
CTTGGCTCTC TACTGAAGAA CCAACAATTC AAGCTGGAGA AACTAATTCT GTGTGTTTGC 
AGTATTAAAG AGAAGCAGTG TGAGATCCTG ACTTCAGCTC TGAAATCAAA CCCATCACAC 
CTGAGAGAAC TGAACCTCAG TGGGAATGAA CTCAAGCACA GAAGAGTGAA GCAATTTTTT 
GATGTGCTGA AAAAATCAAC CTGTAAACTG GAGCGACTGA GGTTAAGATA CTGTAGTATA 
CAAGCCGATG ATTGTACTGA TCTGGCTTCA GCTCTGAAAT CAAACCCATC CCACCTGAGA 
GAACTGGACC TCAGTGGAAA CAATCTAGGA GACTCTGGAG TGAAAAAACT CAGTGATTTA 
CTGATAAACC AACAATTCAA GCTGGAGAAA CTACGTCTGA GTTATTGCAG TATTACAGAG 
AAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
AACCTCAATG GGAATAGACT AGGAGACTCT GGAGCTGATG GCCTTAACCA TCTGCTGAGC 
AGTGTGGATT GCAAGCTAAA TAAACTACAT CTGTGTGATT GCAGTATTAC AGAGAAACAG 
TTGCTCATCC CAATCACAGC TACGTGTATG GGCCTATTAC ACCTGAGAGA ACTGGACCTC 
AGTAAGAACC AAATAAAAAA CCAAGGAGTG TACATGCTGT GTGATGTGCT GAAAAACCCA 
CGCTGTAGAC TCAAGAGGTT AAGACTAAAT GACTGTGGCA TTACAGATGT TACACCCTTA 
ACTCAGTCTT TGACCAAGGA CAAAGCATTG AAGTTCTTAA AAGAGCTTGA ACTGAGTAAA 
AATCAAATGG GATTCTTCGA AAAGCAAAAA CTCAGTGACC TGCTGCGAGA CTCAAACTGT 
AAACTGAGGC TTGAGGAAGA AGAAGGTTAT TTTAGAGCTG GTGTAAAACG GGTTACTACA 
TTTTTGATGC CTCCATGGTC AAAAGTTCAA AAACCTGTGG ATCTCACAGG AGAGGAAAGT 
TCATCATCAG ATGAGGACGC CTCTGAAGAT GAGACTGATC AGGCAGAGGA GGCTGATAAG 
CTCTCAAATG AGGCGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208750.1
CDS	188..9508
Translation	MKMSLSEKHSVRSGSHVSSSVSVKSDRSKGHMPDFSETKTSPNKRFQYKTLEFDFQPQRSCNNFKDLQCIFQGLESKLFNFLKKELENFKKILQKENTQYLVKDFNENRCSIREAILDLTLYFLREMKQDEAADTLEDELIFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVASRRHEAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKKNREISFIFPLPFREMNLKEKETLSLMDLITQFFPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCCDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIERLTEIRGFNDAQKEEYFRKRLTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILQEKRNNGVKNNQADDASKTLQKSNTEDTPKTLTQMYTHFLRFQIQQSRRKYDGENTPDVSWDKDAILSLGKLAFHQLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVVGTMYCFVHLSVQEFIAALYAHLILDINKKSVFDQDSKNRNESMIDLLRTAVDKTLESDNGHLDLFLRFLLGLSLQSNRRLLQGLLTQQEHNDQSKEEIVQYIKQKFEVNHSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSADLSPAQWSALAFVLLTSEEEMEEFELQKFKKSDECLIRLSAVIKTCKRALLNDCNLTDRSCSTVAAVLRSENSLKELNMSNNNLQDSGVKLLCAGLKNIKCDLETLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSAAADEGCQFVTGIVGKNPLLLKELSLSGHELGDTRVNQLSALLQDKHCQINTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACEHLYKVLGGNLLLLRELNLTKHDLGPSGLEKLAAVLKDKHCKLNTLILNNSTISEEDFHVLTEAFNSNPTNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_017353261.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353261.1

ORF Insert Sequence:

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAACGGGGTG ATTGCTGTAA ATTAAAAACC GTCAGGTTTT TAAAGAGTTC TGCTGCACAG 
GAAGCGTGTG ACTATCTTAC TAAAGCTGTG GGTAAAAGTC CATTATTACT GACAAAACTG 
GATCTGAGTG AAGTTAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGAAG 
GACTCTCATA GCAAAGTGGA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCAGTTC TAGCTGCTGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAGATCTGTG AGGGGCTGAA GAATCCTGTG 
TGTGAGCTGA AGATACTCAA TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGGAGCTC ACTGCTTTGA TTCCAGAGTG TGCTCTGAAA 
ACATTGAGGT TTTTGGGTCC TGCAGCAGAA GAAGCCTGTC AGTTTGTGAC TGGAATTGTG 
GGTAAAAACC CGTTACTCCT GAAAGAACTG AATCTGAGTG ATCGTAAACT AGGAGACACA 
CGAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT GTAAACCCAA CACACTTCAG 
CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TAACTTCAGC ATTTAATTTA 
AACCCTTCAC ACCTAATAGA GCTCAATCTG AGTGGGAATA AACTAAGAGA CTCAGGAGTA 
GAGGAGATCT GTCCTCTGCT GAAAAATACA CAGTGCAGAC TGGAGAGACT AAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTAAAGGAG 
CTCACTGACT TACTGCAGGA TGAGCGATAT AGATTGAAGA CCCTCAGGCT TTTGAAAACT 
ACTGCAGCAC AAGAAGCGTG TGACTATCTT ACTAAAGTTG TGGGTAAAAG TCCATTATTA 
CTGACAGAAC TGGATCTGAG TGAAGATAAA TTAGGAGATC TGGATGGAGA GAAACTCTCT 
GCTCTTCTGA TGGACTCTCA TAGTAAAGTG GAGAAAATCA AGCTGAATAA TTGTAAGCAG 
ATTGAAAAAG GCTGTTCAGT ACTAGCTGCC GTTCTCTCCT CAAAAACAAT CCTAAAAGAG 
ATGAACCTGA ACAACAGTCG TCTGCTGGAC TCAGGAGTCT TAGAGATCTG TGAGGGACTG 
AAGAACCCTG TGTGTGAGCT GAAGATTCTC AAGCTCAGTA AGTGTGATTT AACAGAGGAA 
AGCTGTTCAG CTCTGGCTTC AGTTCTCAGA TCAGACTCCA GCAGTCTGAA GGATCTTGAC 
CTGAGCAATA ATAATCTGCA GGATTCAGGA GTGAAGTTGC TCTCTGATGG ACTAAAGCAC 
AGTAAACTGA AGAGACTCAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGGAGCTC ACTGACTTAC TGCAGGATCC ACACTGTACA 
CTAAAGACAC TGAGTTGTTT TAGTCCTGCT GCTGATGAAG GCTGTCAGTT TGTTACTGGA 
ATTGTGGGTA AAAACCCGTT ACTACTGAAA GAACTGAATC TGAGTGATCG TGAGCTAGGA 
GACACACGGG TGAATCAACT CTCTGCTCTA CTGCAAGATA AACACTGTAC ACTCAATATA 
CTCACGCTGA ATAATAACAG GATTACAGCA GAAGGTTGTG CTGCTTTGAC TTCAGCATTT 
AATTTAAACC CTTCACACCT GATAGAGCTC AATCTGAGTG GAAATAAACT AGGAGACTCA 
GGAGTAGAGA AGATCTGTCC TCTGCTGAAA AATACCCAGT GCAGATTGGA GAAATTAAAT 
CTTTCAGACT GCAGCATCAC TGGAAAAGGT TATGAAACAT TGATTTCAGC TTTGAAGTCA 
AACTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGGAAATC AGGAGTGCAG 
AAGCTCACTG ATTTGCTACG AGCTGGAAGC TGTAAATTAA AAACCATCAG GACTTTGAAA 
GGTTCCGCTG CACAGGAAGC GTGTGACTAT CTAACTAAAG CTGTGGGTAA AAGTCCATTA 
TTACTGACAG AACTGGATCT GAGTGAAGAT AAATTAGGAG ATCTGGATGG AGAGAAACTC 
TCTGCTCTTC TGATGGACTC TCATAGCAAA GTGGAGAAAA TCAGGATGAA TAACTGTAAA 
AAGCTGACAG AGAAAAGCTG TTTAGTTCTA GCTGCTGTTC TTTCATCCAA AACCATCTTG 
AAAGAGATGA ACCTAAACAA CAGTCGTCTG CTGGACTCAG GAGTCAAAGT GATCTGTGAG 
GGACTGAAGA ACTCTAAGCT GGAGATTTTA AAACTTTCAA ATTGTAGTAT TACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCTGGA GTAAAGGAGC TCACTGAGTT ACTGCAGAGT 
TCGTGGATTC CATGGAAAAA AGTGAAGTGT TCAATGAGGT TTTTGAGTCC TGCTGCTGAT 
GAATTTTGTC AGTTTGTGTC TGGAATTGTG GGTAAAAACC CGTTACTCCT GAAAGAACTG 
AATCTGAGTG ATCATGTACT AGCAGACACA CAAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GCAAACTCAA CACACTCATT CTTTCAGACT GCAGTATCAG TGGAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGAGATCA AACCCTTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAAGAGCTCA CTGAGTTACT GCAGGATCCA 
CACTGTACAC TGAAGACACT GAGGTTTTTG AGTGATGATG CAGATGAAGC CTGTGAACAT 
TTGTATAAAG TTCTGGGTGG AAACCTGTTA CTCCTGAGAG AGCTGAATCT GACTAAACAT 
GATCTTGGAC CATCAGGATT AGAGAAACTT GCTGCAGTAC TGAAAGATAA ACACTGTAAA 
CTCAACACAC TGATTCTGAA TAACAGCACT ATCTCAGAGG AAGATTTTCA TGTTCTGACT 
GAAGCTTTTA ATTCAAACCC AACAAATCTG ACAGAGCTGA ACTTGAGTGG GACTAAACTC 
ACAAACTCAG GAATGAAGAG CTTCACAACT CTGTTTCAAA ACCAACAGTG TAGATTGGAA 
AAGCTGAAGT TCAGCAGCAT GTGTATTACA GCAGAAGGTT GTGCTGCTTT GACTTCAGCA 
TTTAACTCAA ACCCTTCACA CCTGAAAGAG CTCAATCTGA GTGGGAATAA ACTAGGAGAC 
TCAGGAGTAA GAAAAATCTC CAGTCTACTG AGAAACTCGC AGTGCATGCT GAAGATACTC 
GGTCTTTCAG ACTGCAGTAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT TACATCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACCATCAGGA 
GTGAAGGAGC TCACTGGCTT ACTACTCGAT CCACACTGTA CACTGACGAC ACTGAGGTTT 
TTGAGTCCTG CTGCTGATGA ATTCTGTCAG TTTGTGTCTG GAATTGTGGG TAAATACCCA 
TTACTCCTGA AAGAACTGAA TCTGAGTGAT CGTGGACTAG GGGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTTT AGACTCAACA CACTCATGCT GAATAATAAC 
AGGATTACAG CAGAAGGTTG TGCTGCTTTA ACTTCAGCAT TTAATTTAAA TCCTTCACAC 
CTGATAGAGC TCAATCTGAG TGGGAATAAA CTAGAAGACT CAGGAGTAGA GAAGATCTGT 
CCTCTGCTGG AAAATATACA ATGCCGATTA GAGAAACTGA GTCTTTCAGA CTGCAGTATC 
AGGGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CTGCAGAGTA GAAGCTGTAA ATTCAGGTTT CTGAAGAGTT CTGCTGCACA GGAAGCATGC 
GAGTATCTTA CTAATGTTCT GGGTATAAGT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGTTAAAT TAGGAGATCT GGACTGGGAG AAACTCTCTG CTCTTCTGAT GGACTCTCAT 
AGCAAAGTGG AGAAAATCAA GCTAAATAAT TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT 
CTAGCTGCTG TTCTCTCCTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AAAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA ATCTTTCAGA CTGCAGTATT ACAGAAGAAG GTTATGAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTGC ACTGAAGACA 
CTGAGATTTT TAAGAAGTCC TGCTGCTGAT GAAGGCTGTC AGTTAGTAAC TGGAGTTGTG 
GGTAAAAACC CGTTACTCCT GAAGGAACTG AATCTGAGTG ATCATGAGCT AGGAGACACA 
CGAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT GCCAGATCAA CACACTCAAT 
CTGAGTTATT GCAGTATTAC AGAGAAACAG TGTGAGATCC TGACTTCAGC TCTGAAATCA 
AACCCATCAC ACCTGAGAGA ACTGGACCTC AGTGGGAATG AACTAGGAGA CTTTCAAAGC 
CTTGGCTCTC TACTGAAGAA CCAACAATTC AAGCTGGAGA AACTAATTCT GTGTGTTTGC 
AGTATTAAAG AGAAGCAGTG TGAGATCCTG ACTTCAGCTC TGAAATCAAA CCCATCACAC 
CTGAGAGAAC TGAACCTCAG TGGGAATGAA CTCAAGCACA GAAGAGTGAA GCAATTTTTT 
GATGTGCTGA AAAAATCAAC CTGTAAACTG GAGCGACTGA GGTTAAGATA CTGTAGTATA 
CAAGCCGATG ATTGTACTGA TCTGGCTTCA GCTCTGAAAT CAAACCCATC CCACCTGAGA 
GAACTGGACC TCAGTGGAAA CAATCTAGGA GACTCTGGAG TGAAAAAACT CAGTGATTTA 
CTGATAAACC AACAATTCAA GCTGGAGAAA CTACGTCTGA GTTATTGCAG TATTACAGAG 
AAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
AACCTCAATG GGAATAGACT AGGAGACTCT GGAGCTGATG GCCTTAACCA TCTGCTGAGC 
AGTGTGGATT GCAAGCTAAA TAAACTACAT CTGTGTGATT GCAGTATTAC AGAGAAACAG 
TTGCTCATCC CAATCACAGC TACGTGTATG GGCCTATTAC ACCTGAGAGA ACTGGACCTC 
AGTAAGAACC AAATAAAAAA CCAAGGAGTG TACATGCTGT GTGATGTGCT GAAAAACCCA 
CGCTGTAGAC TCAAGAGGTT AAGACTAAAT GACTGTGGCA TTACAGATGT TACACCCTTA 
ACTCAGTCTT TGACCAAGGA CAAAGCATTG AAGTTCTTAA AAGAGCTTGA ACTGAGTAAA 
AATCAAATGG GATTCTTCGA AAAGCAAAAA CTCAGTGACC TGCTGCGAGA CTCAAACTGT 
AAACTGAGGC TTGAGGAAGA AGAAGGTTAT TTTAGAGCTG GTGTAAAACG GGTTACTACA 
TTTTTGATGC CTCCATGGTC AAAAGTTCAA AAACCTGTGG ATCTCACAGG AGAGGAAAGT 
TCATCATCAG ATGAGGACGC CTCTGAAGAT GAGACTGATC AGGCAGAGGA GGCTGATAAG 
CTCTCAAATG AGGCGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_017353262.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208751.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X3, mRNA.

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CloneID	ODa57133
Clone ID Related Accession (Same CDS sequence)	XM_017353262.1
Accession Version	XM_017353262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein si:ch211-11p18.6 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAACGGGGTG ATTGCTGTAA ATTAAAAACC GTCAGGTTTT TAAAGAGTTC TGCTGCACAG 
GAAGCGTGTG ACTATCTTAC TAAAGCTGTG GGTAAAAGTC CATTATTACT GACAAAACTG 
GATCTGAGTG AAGTTAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGAAG 
GACTCTCATA GCAAAGTGGA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCAGTTC TAGCTGCTGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAGATCTGTG AGGGGCTGAA GAATCCTGTG 
TGTGAGCTGA AGATACTCAA CCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACTTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGACTTAC TGCAGGATCC ACACTGTACC 
CTGAATACAC TGAGGTTTTT GAGTCCTGCC GCAGAAGAAG CCTGTCAGTT TGTGACTGGA 
ATTGTGGGTA AAAACCCGAT ACTTCTGAAA GAACTGAATC TGAGTGATCA TGAACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGTAA ACTCAACACA 
CTGATTCTGA AAAACAGCTT TATCTCAGAG GAAGATTGTC GTGTTCTGGC TGAAGCTTTT 
AATTCAAACC CATCAAATCT GAGAGAGCTG AATTTGAATG GGACTAAACT CACAAACTCA 
GGAATGAAGA GCTTCAACAC TCTGTTTCAA AACCAACAGT GCAGACTGGA AAAGCTGAAG 
TTTAGCGGCA TCAGTATCAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAGCTCA 
AACCCGTCAC ACCTGATAGA GCTAAATCTG AGTGGGAATA CACTAGAAGA CTCAGGAGTA 
ACAGAAATCT CCAGTCTACT GGCAAACTCA CAGTGCATGC TGAAGATACT CAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGCTT TGATTCCAGA GTGTGCTCTG AAAACATTGA GGTTTTTGGG TCCTGCAGCA 
GAAGAAGCCT GTCAGTTTGT GACTGGAATT GTGGGTAAAA ACCCGTTACT CCTGAAAGAA 
CTGAATCTGA GTGATCGTAA ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTACTG 
CAGGATAAAC ACTGTAAACC CAACACACTT CAGCTGAATA ATAACAGGAT TACAGCAGAA 
GGTTGTGCTG CTTTAACTTC AGCATTTAAT TTAAACCCTT CACACCTAAT AGAGCTCAAT 
CTGAGTGGGA ATAAACTAAG AGACTCAGGA GTAGAGGAGA TCTGTCCTCT GCTGAAAAAT 
ACACAGTGCA GACTGGAGAG ACTAAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTAAAG GAGCTCACTG ACTTACTGCA GGATGAGCGA 
TATAGATTGA AGACCCTCAG GCTTTTGAAA ACTACTGCAG CACAAGAAGC GTGTGACTAT 
CTTACTAAAG TTGTGGGTAA AAGTCCATTA TTACTGACAG AACTGGATCT GAGTGAAGAT 
AAATTAGGAG ATCTGGATGG AGAGAAACTC TCTGCTCTTC TGATGGACTC TCATAGTAAA 
GTGGAGAAAA TCAAGCTGAA TAATTGTAAG CAGATTGAAA AAGGCTGTTC AGTACTAGCT 
GCCGTTCTCT CCTCAAAAAC AATCCTAAAA GAGATGAACC TGAACAACAG TCGTCTGCTG 
GACTCAGGAG TCTTAGAGAT CTGTGAGGGA CTGAAGAACC CTGTGTGTGA GCTGAAGATT 
CTCAAGCTCA GTAAGTGTGA TTTAACAGAG GAAAGCTGTT CAGCTCTGGC TTCAGTTCTC 
AGATCAGACT CCAGCAGTCT GAAGGATCTT GACCTGAGCA ATAATAATCT GCAGGATTCA 
GGAGTGAAGT TGCTCTCTGA TGGACTAAAG CACAGTAAAC TGAAGAGACT CAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGACT TACTGCAGGA TCCACACTGT ACACTAAAGA CACTGAGTTG TTTTAGTCCT 
GCTGCTGATG AAGGCTGTCA GTTTGTTACT GGAATTGTGG GTAAAAACCC GTTACTACTG 
AAAGAACTGA ATCTGAGTGA TCGTGAGCTA GGAGACACAC GGGTGAATCA ACTCTCTGCT 
CTACTGCAAG ATAAACACTG TACACTCAAT ATACTCACGC TGAATAATAA CAGGATTACA 
GCAGAAGGTT GTGCTGCTTT GACTTCAGCA TTTAATTTAA ACCCTTCACA CCTGATAGAG 
CTCAATCTGA GTGGAAATAA ACTAGGAGAC TCAGGAGTAG AGAAGATCTG TCCTCTGCTG 
AAAAATACCC AGTGCAGATT GGAGAAATTA AATCTTTCAG ACTGCAGCAT CACTGGAAAA 
GGTTATGAAA CATTGATTTC AGCTTTGAAG TCAAACTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGGAA ATCAGGAGTG CAGAAGCTCA CTGATTTGCT ACGAGCTGGA 
AGCTGTAAAT TAAAAACCAT CAGGACTTTG AAAGGTTCCG CTGCACAGGA AGCGTGTGAC 
TATCTAACTA AAGCTGTGGG TAAAAGTCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA CTCTCTGCTC TTCTGATGGA CTCTCATAGC 
AAAGTGGAGA AAATCAGGAT GAATAACTGT AAAAAGCTGA CAGAGAAAAG CTGTTTAGTT 
CTAGCTGCTG TTCTTTCATC CAAAACCATC TTGAAAGAGA TGAACCTAAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAA AGTGATCTGT GAGGGACTGA AGAACTCTAA GCTGGAGATT 
TTAAAACTTT CAAATTGTAG TATTACAGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCT 
GGAGTAAAGG AGCTCACTGA GTTACTGCAG AGTTCGTGGA TTCCATGGAA AAAAGTGAAG 
TGTTCAATGA GGTTTTTGAG TCCTGCTGCT GATGAATTTT GTCAGTTTGT GTCTGGAATT 
GTGGGTAAAA ACCCGTTACT CCTGAAAGAA CTGAATCTGA GTGATCATGT ACTAGCAGAC 
ACACAAGTGA ATCAACTCTC TGCTCTACTG CAGGATAAAC ACTGCAAACT CAACACACTC 
ATTCTTTCAG ACTGCAGTAT CAGTGGAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAAGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CTGCTGATGA ATTCTGTCAG TTTGTGTCTG GAATTGTGGG TAAATACCCA 
TTACTCCTGA AAGAACTGAA TCTGAGTGAT CGTGGACTAG GGGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTTT AGACTCAACA CACTCATGCT GAATAATAAC 
AGGATTACAG CAGAAGGTTG TGCTGCTTTA ACTTCAGCAT TTAATTTAAA TCCTTCACAC 
CTGATAGAGC TCAATCTGAG TGGGAATAAA CTAGAAGACT CAGGAGTAGA GAAGATCTGT 
CCTCTGCTGG AAAATATACA ATGCCGATTA GAGAAACTGA GTCTTTCAGA CTGCAGTATC 
AGGGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CTGCAGAGTA GAAGCTGTAA ATTCAGGTTT CTGAAGAGTT CTGCTGCACA GGAAGCATGC 
GAGTATCTTA CTAATGTTCT GGGTATAAGT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGTTAAAT TAGGAGATCT GGACTGGGAG AAACTCTCTG CTCTTCTGAT GGACTCTCAT 
AGCAAAGTGG AGAAAATCAA GCTAAATAAT TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT 
CTAGCTGCTG TTCTCTCCTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AAAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA ATCTTTCAGA CTGCAGTATT ACAGAAGAAG GTTATGAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTGC ACTGAAGACA 
CTGAGATTTT TAAGAAGTCC TGCTGCTGAT GAAGGCTGTC AGTTAGTAAC TGGAGTTGTG 
GGTAAAAACC CGTTACTCCT GAAGGAACTG AATCTGAGTG ATCATGAGCT AGGAGACACA 
CGAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT GCCAGATCAA CACACTCAAT 
CTGAGTTATT GCAGTATTAC AGAGAAACAG TGTGAGATCC TGACTTCAGC TCTGAAATCA 
AACCCATCAC ACCTGAGAGA ACTGGACCTC AGTGGGAATG AACTAGGAGA CTTTCAAAGC 
CTTGGCTCTC TACTGAAGAA CCAACAATTC AAGCTGGAGA AACTAATTCT GTGTGTTTGC 
AGTATTAAAG AGAAGCAGTG TGAGATCCTG ACTTCAGCTC TGAAATCAAA CCCATCACAC 
CTGAGAGAAC TGAACCTCAG TGGGAATGAA CTCAAGCACA GAAGAGTGAA GCAATTTTTT 
GATGTGCTGA AAAAATCAAC CTGTAAACTG GAGCGACTGA GGTTAAGATA CTGTAGTATA 
CAAGCCGATG ATTGTACTGA TCTGGCTTCA GCTCTGAAAT CAAACCCATC CCACCTGAGA 
GAACTGGACC TCAGTGGAAA CAATCTAGGA GACTCTGGAG TGAAAAAACT CAGTGATTTA 
CTGATAAACC AACAATTCAA GCTGGAGAAA CTACGTCTGA GTTATTGCAG TATTACAGAG 
AAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
AACCTCAATG GGAATAGACT AGGAGACTCT GGAGCTGATG GCCTTAACCA TCTGCTGAGC 
AGTGTGGATT GCAAGCTAAA TAAACTACAT CTGTGTGATT GCAGTATTAC AGAGAAACAG 
TTGCTCATCC CAATCACAGC TACGTGTATG GGCCTATTAC ACCTGAGAGA ACTGGACCTC 
AGTAAGAACC AAATAAAAAA CCAAGGAGTG TACATGCTGT GTGATGTGCT GAAAAACCCA 
CGCTGTAGAC TCAAGAGGTT AAGACTAAAT GACTGTGGCA TTACAGATGT TACACCCTTA 
ACTCAGTCTT TGACCAAGGA CAAAGCATTG AAGTTCTTAA AAGAGCTTGA ACTGAGTAAA 
AATCAAATGG GATTCTTCGA AAAGCAAAAA CTCAGTGACC TGCTGCGAGA CTCAAACTGT 
AAACTGAGGC TTGAGGAAGA AGAAGGTTAT TTTAGAGCTG GTGTAAAACG GGTTACTACA 
TTTTTGATGC CTCCATGGTC AAAAGTTCAA AAACCTGTGG ATCTCACAGG AGAGGAAAGT 
TCATCATCAG ATGAGGACGC CTCTGAAGAT GAGACTGATC AGGCAGAGGA GGCTGATAAG 
CTCTCAAATG AGGCGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208751.1
CDS	188..9508
Translation	MKMSLSEKHSVRSGSHVSSSVSVKSDRSKGHMPDFSETKTSPNKRFQYKTLEFDFQPQRSCNNFKDLQCIFQGLESKLFNFLKKELENFKKILQKENTQYLVKDFNENRCSIREAILDLTLYFLREMKQDEAADTLEDELIFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVASRRHEAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKKNREISFIFPLPFREMNLKEKETLSLMDLITQFFPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCCDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIERLTEIRGFNDAQKEEYFRKRLTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILQEKRNNGVKNNQADDASKTLQKSNTEDTPKTLTQMYTHFLRFQIQQSRRKYDGENTPDVSWDKDAILSLGKLAFHQLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVVGTMYCFVHLSVQEFIAALYAHLILDINKKSVFDQDSKNRNESMIDLLRTAVDKTLESDNGHLDLFLRFLLGLSLQSNRRLLQGLLTQQEHNDQSKEEIVQYIKQKFEVNHSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSADLSPAQWSALAFVLLTSEEEMEEFELQKFKKSDECLIRLSAVIKTCKRALLNDCNLTDRSCSTVAAVLRSENSLKELNMSNNNLQDSGVKLLCAGLKNIKCDLETLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSAAADEGCQFVTGIVGKNPLLLKELSLSGHELGDTRVNQLSALLQDKHCQINTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLKFSGISITAEGCAALTSAFSSNPSHLIELNLSGNTLEDSGVTEISSLLANSQCMLKILSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_017353262.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353262.1

ORF Insert Sequence:

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAACGGGGTG ATTGCTGTAA ATTAAAAACC GTCAGGTTTT TAAAGAGTTC TGCTGCACAG 
GAAGCGTGTG ACTATCTTAC TAAAGCTGTG GGTAAAAGTC CATTATTACT GACAAAACTG 
GATCTGAGTG AAGTTAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGAAG 
GACTCTCATA GCAAAGTGGA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCAGTTC TAGCTGCTGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAGATCTGTG AGGGGCTGAA GAATCCTGTG 
TGTGAGCTGA AGATACTCAA CCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACTTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGACTTAC TGCAGGATCC ACACTGTACC 
CTGAATACAC TGAGGTTTTT GAGTCCTGCC GCAGAAGAAG CCTGTCAGTT TGTGACTGGA 
ATTGTGGGTA AAAACCCGAT ACTTCTGAAA GAACTGAATC TGAGTGATCA TGAACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGTAA ACTCAACACA 
CTGATTCTGA AAAACAGCTT TATCTCAGAG GAAGATTGTC GTGTTCTGGC TGAAGCTTTT 
AATTCAAACC CATCAAATCT GAGAGAGCTG AATTTGAATG GGACTAAACT CACAAACTCA 
GGAATGAAGA GCTTCAACAC TCTGTTTCAA AACCAACAGT GCAGACTGGA AAAGCTGAAG 
TTTAGCGGCA TCAGTATCAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAGCTCA 
AACCCGTCAC ACCTGATAGA GCTAAATCTG AGTGGGAATA CACTAGAAGA CTCAGGAGTA 
ACAGAAATCT CCAGTCTACT GGCAAACTCA CAGTGCATGC TGAAGATACT CAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGCTT TGATTCCAGA GTGTGCTCTG AAAACATTGA GGTTTTTGGG TCCTGCAGCA 
GAAGAAGCCT GTCAGTTTGT GACTGGAATT GTGGGTAAAA ACCCGTTACT CCTGAAAGAA 
CTGAATCTGA GTGATCGTAA ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTACTG 
CAGGATAAAC ACTGTAAACC CAACACACTT CAGCTGAATA ATAACAGGAT TACAGCAGAA 
GGTTGTGCTG CTTTAACTTC AGCATTTAAT TTAAACCCTT CACACCTAAT AGAGCTCAAT 
CTGAGTGGGA ATAAACTAAG AGACTCAGGA GTAGAGGAGA TCTGTCCTCT GCTGAAAAAT 
ACACAGTGCA GACTGGAGAG ACTAAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTAAAG GAGCTCACTG ACTTACTGCA GGATGAGCGA 
TATAGATTGA AGACCCTCAG GCTTTTGAAA ACTACTGCAG CACAAGAAGC GTGTGACTAT 
CTTACTAAAG TTGTGGGTAA AAGTCCATTA TTACTGACAG AACTGGATCT GAGTGAAGAT 
AAATTAGGAG ATCTGGATGG AGAGAAACTC TCTGCTCTTC TGATGGACTC TCATAGTAAA 
GTGGAGAAAA TCAAGCTGAA TAATTGTAAG CAGATTGAAA AAGGCTGTTC AGTACTAGCT 
GCCGTTCTCT CCTCAAAAAC AATCCTAAAA GAGATGAACC TGAACAACAG TCGTCTGCTG 
GACTCAGGAG TCTTAGAGAT CTGTGAGGGA CTGAAGAACC CTGTGTGTGA GCTGAAGATT 
CTCAAGCTCA GTAAGTGTGA TTTAACAGAG GAAAGCTGTT CAGCTCTGGC TTCAGTTCTC 
AGATCAGACT CCAGCAGTCT GAAGGATCTT GACCTGAGCA ATAATAATCT GCAGGATTCA 
GGAGTGAAGT TGCTCTCTGA TGGACTAAAG CACAGTAAAC TGAAGAGACT CAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGACT TACTGCAGGA TCCACACTGT ACACTAAAGA CACTGAGTTG TTTTAGTCCT 
GCTGCTGATG AAGGCTGTCA GTTTGTTACT GGAATTGTGG GTAAAAACCC GTTACTACTG 
AAAGAACTGA ATCTGAGTGA TCGTGAGCTA GGAGACACAC GGGTGAATCA ACTCTCTGCT 
CTACTGCAAG ATAAACACTG TACACTCAAT ATACTCACGC TGAATAATAA CAGGATTACA 
GCAGAAGGTT GTGCTGCTTT GACTTCAGCA TTTAATTTAA ACCCTTCACA CCTGATAGAG 
CTCAATCTGA GTGGAAATAA ACTAGGAGAC TCAGGAGTAG AGAAGATCTG TCCTCTGCTG 
AAAAATACCC AGTGCAGATT GGAGAAATTA AATCTTTCAG ACTGCAGCAT CACTGGAAAA 
GGTTATGAAA CATTGATTTC AGCTTTGAAG TCAAACTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGGAA ATCAGGAGTG CAGAAGCTCA CTGATTTGCT ACGAGCTGGA 
AGCTGTAAAT TAAAAACCAT CAGGACTTTG AAAGGTTCCG CTGCACAGGA AGCGTGTGAC 
TATCTAACTA AAGCTGTGGG TAAAAGTCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA CTCTCTGCTC TTCTGATGGA CTCTCATAGC 
AAAGTGGAGA AAATCAGGAT GAATAACTGT AAAAAGCTGA CAGAGAAAAG CTGTTTAGTT 
CTAGCTGCTG TTCTTTCATC CAAAACCATC TTGAAAGAGA TGAACCTAAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAA AGTGATCTGT GAGGGACTGA AGAACTCTAA GCTGGAGATT 
TTAAAACTTT CAAATTGTAG TATTACAGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCT 
GGAGTAAAGG AGCTCACTGA GTTACTGCAG AGTTCGTGGA TTCCATGGAA AAAAGTGAAG 
TGTTCAATGA GGTTTTTGAG TCCTGCTGCT GATGAATTTT GTCAGTTTGT GTCTGGAATT 
GTGGGTAAAA ACCCGTTACT CCTGAAAGAA CTGAATCTGA GTGATCATGT ACTAGCAGAC 
ACACAAGTGA ATCAACTCTC TGCTCTACTG CAGGATAAAC ACTGCAAACT CAACACACTC 
ATTCTTTCAG ACTGCAGTAT CAGTGGAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAAGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CTGCTGATGA ATTCTGTCAG TTTGTGTCTG GAATTGTGGG TAAATACCCA 
TTACTCCTGA AAGAACTGAA TCTGAGTGAT CGTGGACTAG GGGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTTT AGACTCAACA CACTCATGCT GAATAATAAC 
AGGATTACAG CAGAAGGTTG TGCTGCTTTA ACTTCAGCAT TTAATTTAAA TCCTTCACAC 
CTGATAGAGC TCAATCTGAG TGGGAATAAA CTAGAAGACT CAGGAGTAGA GAAGATCTGT 
CCTCTGCTGG AAAATATACA ATGCCGATTA GAGAAACTGA GTCTTTCAGA CTGCAGTATC 
AGGGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CTGCAGAGTA GAAGCTGTAA ATTCAGGTTT CTGAAGAGTT CTGCTGCACA GGAAGCATGC 
GAGTATCTTA CTAATGTTCT GGGTATAAGT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGTTAAAT TAGGAGATCT GGACTGGGAG AAACTCTCTG CTCTTCTGAT GGACTCTCAT 
AGCAAAGTGG AGAAAATCAA GCTAAATAAT TGTGAGCTGA CAGAGAAAAG CTGTTCAGTT 
CTAGCTGCTG TTCTCTCCTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AAAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA ATCTTTCAGA CTGCAGTATT ACAGAAGAAG GTTATGAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTGC ACTGAAGACA 
CTGAGATTTT TAAGAAGTCC TGCTGCTGAT GAAGGCTGTC AGTTAGTAAC TGGAGTTGTG 
GGTAAAAACC CGTTACTCCT GAAGGAACTG AATCTGAGTG ATCATGAGCT AGGAGACACA 
CGAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT GCCAGATCAA CACACTCAAT 
CTGAGTTATT GCAGTATTAC AGAGAAACAG TGTGAGATCC TGACTTCAGC TCTGAAATCA 
AACCCATCAC ACCTGAGAGA ACTGGACCTC AGTGGGAATG AACTAGGAGA CTTTCAAAGC 
CTTGGCTCTC TACTGAAGAA CCAACAATTC AAGCTGGAGA AACTAATTCT GTGTGTTTGC 
AGTATTAAAG AGAAGCAGTG TGAGATCCTG ACTTCAGCTC TGAAATCAAA CCCATCACAC 
CTGAGAGAAC TGAACCTCAG TGGGAATGAA CTCAAGCACA GAAGAGTGAA GCAATTTTTT 
GATGTGCTGA AAAAATCAAC CTGTAAACTG GAGCGACTGA GGTTAAGATA CTGTAGTATA 
CAAGCCGATG ATTGTACTGA TCTGGCTTCA GCTCTGAAAT CAAACCCATC CCACCTGAGA 
GAACTGGACC TCAGTGGAAA CAATCTAGGA GACTCTGGAG TGAAAAAACT CAGTGATTTA 
CTGATAAACC AACAATTCAA GCTGGAGAAA CTACGTCTGA GTTATTGCAG TATTACAGAG 
AAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
AACCTCAATG GGAATAGACT AGGAGACTCT GGAGCTGATG GCCTTAACCA TCTGCTGAGC 
AGTGTGGATT GCAAGCTAAA TAAACTACAT CTGTGTGATT GCAGTATTAC AGAGAAACAG 
TTGCTCATCC CAATCACAGC TACGTGTATG GGCCTATTAC ACCTGAGAGA ACTGGACCTC 
AGTAAGAACC AAATAAAAAA CCAAGGAGTG TACATGCTGT GTGATGTGCT GAAAAACCCA 
CGCTGTAGAC TCAAGAGGTT AAGACTAAAT GACTGTGGCA TTACAGATGT TACACCCTTA 
ACTCAGTCTT TGACCAAGGA CAAAGCATTG AAGTTCTTAA AAGAGCTTGA ACTGAGTAAA 
AATCAAATGG GATTCTTCGA AAAGCAAAAA CTCAGTGACC TGCTGCGAGA CTCAAACTGT 
AAACTGAGGC TTGAGGAAGA AGAAGGTTAT TTTAGAGCTG GTGTAAAACG GGTTACTACA 
TTTTTGATGC CTCCATGGTC AAAAGTTCAA AAACCTGTGG ATCTCACAGG AGAGGAAAGT 
TCATCATCAG ATGAGGACGC CTCTGAAGAT GAGACTGATC AGGCAGAGGA GGCTGATAAG 
CTCTCAAATG AGGCGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_017353263.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208752.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X4, mRNA.

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CloneID	ODa57134
Clone ID Related Accession (Same CDS sequence)	XM_017353263.1
Accession Version	XM_017353263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	protein NLRC5 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGAGTTA 
CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGTCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGC TTTGATTCCA 
GAGTGTGCTC TGAAAACATT GAGGTTTTTG GGTCCTGCAG CAGAAGAAGC CTGTCAGTTT 
GTGACTGGAA TTGTGGGTAA AAACCCGTTA CTCCTGAAAG AACTGAATCT GAGTGATCGT 
AAACTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGTAAA 
CCCAACACAC TTCAGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTAACT 
TCAGCATTTA ATTTAAACCC TTCACACCTA ATAGAGCTCA ATCTGAGTGG GAATAAACTA 
AGAGACTCAG GAGTAGAGGA GATCTGTCCT CTGCTGAAAA ATACACAGTG CAGACTGGAG 
AGACTAAGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTAA AGGAGCTCAC TGACTTACTG CAGGATGAGC GATATAGATT GAAGACCCTC 
AGGCTTTTGA AAACTACTGC AGCACAAGAA GCGTGTGACT ATCTTACTAA AGTTGTGGGT 
AAAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG ATAAATTAGG AGATCTGGAT 
GGAGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGTA AAGTGGAGAA AATCAAGCTG 
AATAATTGTA AGCAGATTGA AAAAGGCTGT TCAGTACTAG CTGCCGTTCT CTCCTCAAAA 
ACAATCCTAA AAGAGATGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCTTAGAG 
ATCTGTGAGG GACTGAAGAA CCCTGTGTGT GAGCTGAAGA TTCTCAAGCT CAGTAAGTGT 
GATTTAACAG AGGAAAGCTG TTCAGCTCTG GCTTCAGTTC TCAGATCAGA CTCCAGCAGT 
CTGAAGGATC TTGACCTGAG CAATAATAAT CTGCAGGATT CAGGAGTGAA GTTGCTCTCT 
GATGGACTAA AGCACAGTAA ACTGAAGAGA CTCAGTCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA CTTACTGCAG 
GATCCACACT GTACACTAAA GACACTGAGT TGTTTTAGTC CTGCTGCTGA TGAAGGCTGT 
CAGTTTGTTA CTGGAATTGT GGGTAAAAAC CCGTTACTAC TGAAAGAACT GAATCTGAGT 
GATCGTGAGC TAGGAGACAC ACGGGTGAAT CAACTCTCTG CTCTACTGCA AGATAAACAC 
TGTACACTCA ATATACTCAC GCTGAATAAT AACAGGATTA CAGCAGAAGG TTGTGCTGCT 
TTGACTTCAG CATTTAATTT AAACCCTTCA CACCTGATAG AGCTCAATCT GAGTGGAAAT 
AAACTAGGAG ACTCAGGAGT AGAGAAGATC TGTCCTCTGC TGAAAAATAC CCAGTGCAGA 
TTGGAGAAAT TAAATCTTTC AGACTGCAGC ATCACTGGAA AAGGTTATGA AACATTGATT 
TCAGCTTTGA AGTCAAACTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGG 
AAATCAGGAG TGCAGAAGCT CACTGATTTG CTACGAGCTG GAAGCTGTAA ATTAAAAACC 
ATCAGGACTT TGAAAGGTTC CGCTGCACAG GAAGCGTGTG ACTATCTAAC TAAAGCTGTG 
GGTAAAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG 
GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGGA GAAAATCAGG 
ATGAATAACT GTAAAAAGCT GACAGAGAAA AGCTGTTTAG TTCTAGCTGC TGTTCTTTCA 
TCCAAAACCA TCTTGAAAGA GATGAACCTA AACAACAGTC GTCTGCTGGA CTCAGGAGTC 
AAAGTGATCT GTGAGGGACT GAAGAACTCT AAGCTGGAGA TTTTAAAACT TTCAAATTGT 
AGTATTACAG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CTGGAGTAAA GGAGCTCACT 
GAGTTACTGC AGAGTTCGTG GATTCCATGG AAAAAAGTGA AGTGTTCAAT GAGGTTTTTG 
AGTCCTGCTG CTGATGAATT TTGTCAGTTT GTGTCTGGAA TTGTGGGTAA AAACCCGTTA 
CTCCTGAAAG AACTGAATCT GAGTGATCAT GTACTAGCAG ACACACAAGT GAATCAACTC 
TCTGCTCTAC TGCAGGATAA ACACTGCAAA CTCAACACAC TCATTCTTTC AGACTGCAGT 
ATCAGTGGAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAAGA GCTCACTGAG 
TTACTGCAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTGA TGATGCAGAT 
GAAGCCTGTG AACATTTGTA TAAAGTTCTG GGTGGAAACC TGTTACTCCT GAGAGAGCTG 
AATCTGACTA AACATGATCT TGGACCATCA GGATTAGAGA AACTTGCTGC AGTACTGAAA 
GATAAACACT GTAAACTCAA CACACTGATT CTGAATAACA GCACTATCTC AGAGGAAGAT 
TTTCATGTTC TGACTGAAGC TTTTAATTCA AACCCAACAA ATCTGACAGA GCTGAACTTG 
AGTGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA CAACTCTGTT TCAAAACCAA 
CAGTGTAGAT TGGAAAAGCT GAAGTTCAGC AGCATGTGTA TTACAGCAGA AGGTTGTGCT 
GCTTTGACTT CAGCATTTAA CTCAAACCCT TCACACCTGA AAGAGCTCAA TCTGAGTGGG 
AATAAACTAG GAGACTCAGG AGTAAGAAAA ATCTCCAGTC TACTGAGAAA CTCGCAGTGC 
ATGCTGAAGA TACTCGGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTTACAT CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACCAT CAGGAGTGAA GGAGCTCACT GGCTTACTAC TCGATCCACA CTGTACACTG 
ACGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAATTCT GTCAGTTTGT GTCTGGAATT 
GTGGGTAAAT ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGG ACTAGGGGAC 
ACACGAGTGA ATCAACTCTC TGCTCTACTG CAGGATAAAC ACTTTAGACT CAACACACTC 
ATGCTGAATA ATAACAGGAT TACAGCAGAA GGTTGTGCTG CTTTAACTTC AGCATTTAAT 
TTAAATCCTT CACACCTGAT AGAGCTCAAT CTGAGTGGGA ATAAACTAGA AGACTCAGGA 
GTAGAGAAGA TCTGTCCTCT GCTGGAAAAT ATACAATGCC GATTAGAGAA ACTGAGTCTT 
TCAGACTGCA GTATCAGGGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG ATTTACTGCA GAGTAGAAGC TGTAAATTCA GGTTTCTGAA GAGTTCTGCT 
GCACAGGAAG CATGCGAGTA TCTTACTAAT GTTCTGGGTA TAAGTCCATT ATTACTGACA 
GAACTGGATC TGAGTGAAGT TAAATTAGGA GATCTGGACT GGGAGAAACT CTCTGCTCTT 
CTGATGGACT CTCATAGCAA AGTGGAGAAA ATCAAGCTAA ATAATTGTGA GCTGACAGAG 
AAAAGCTGTT CAGTTCTAGC TGCTGTTCTC TCCTCCAAAA CCATCCTGAA AGAGCTGAAC 
CTGAACAACA GTCGTCTGCT GGACTCAGGA GTCAGAAAGA TCTGTGAGGG ACTGAAGAAT 
CCTGTGTGTG AGCTGAAGAT ACTCAATCTT TCAGACTGCA GTATTACAGA AGAAGGTTAT 
GAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTGCACTGA AGACACTGAG ATTTTTAAGA AGTCCTGCTG CTGATGAAGG CTGTCAGTTA 
GTAACTGGAG TTGTGGGTAA AAACCCGTTA CTCCTGAAGG AACTGAATCT GAGTGATCAT 
GAGCTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGCCAG 
ATCAACACAC TCAATCTGAG TTATTGCAGT ATTACAGAGA AACAGTGTGA GATCCTGACT 
TCAGCTCTGA AATCAAACCC ATCACACCTG AGAGAACTGG ACCTCAGTGG GAATGAACTA 
GGAGACTTTC AAAGCCTTGG CTCTCTACTG AAGAACCAAC AATTCAAGCT GGAGAAACTA 
ATTCTGTGTG TTTGCAGTAT TAAAGAGAAG CAGTGTGAGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGGA ATGAACTCAA GCACAGAAGA 
GTGAAGCAAT TTTTTGATGT GCTGAAAAAA TCAACCTGTA AACTGGAGCG ACTGAGGTTA 
AGATACTGTA GTATACAAGC CGATGATTGT ACTGATCTGG CTTCAGCTCT GAAATCAAAC 
CCATCCCACC TGAGAGAACT GGACCTCAGT GGAAACAATC TAGGAGACTC TGGAGTGAAA 
AAACTCAGTG ATTTACTGAT AAACCAACAA TTCAAGCTGG AGAAACTACG TCTGAGTTAT 
TGCAGTATTA CAGAGAAACA GTGTGTGATC CTGACTTCAG CTCTGAAATC AAACCCATCA 
CACCTGAGAG AACTGAACCT CAATGGGAAT AGACTAGGAG ACTCTGGAGC TGATGGCCTT 
AACCATCTGC TGAGCAGTGT GGATTGCAAG CTAAATAAAC TACATCTGTG TGATTGCAGT 
ATTACAGAGA AACAGTTGCT CATCCCAATC ACAGCTACGT GTATGGGCCT ATTACACCTG 
AGAGAACTGG ACCTCAGTAA GAACCAAATA AAAAACCAAG GAGTGTACAT GCTGTGTGAT 
GTGCTGAAAA ACCCACGCTG TAGACTCAAG AGGTTAAGAC TAAATGACTG TGGCATTACA 
GATGTTACAC CCTTAACTCA GTCTTTGACC AAGGACAAAG CATTGAAGTT CTTAAAAGAG 
CTTGAACTGA GTAAAAATCA AATGGGATTC TTCGAAAAGC AAAAACTCAG TGACCTGCTG 
CGAGACTCAA ACTGTAAACT GAGGCTTGAG GAAGAAGAAG GTTATTTTAG AGCTGGTGTA 
AAACGGGTTA CTACATTTTT GATGCCTCCA TGGTCAAAAG TTCAAAAACC TGTGGATCTC 
ACAGGAGAGG AAAGTTCATC ATCAGATGAG GACGCCTCTG AAGATGAGAC TGATCAGGCA 
GAGGAGGCTG ATAAGCTCTC AAATGAGGCG GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208752.1
CDS	188..9163
Translation	MKMSLSEKHSVRSGSHVSSSVSVKSDRSKGHMPDFSETKTSPNKRFQYKTLEFDFQPQRSCNNFKDLQCIFQGLESKLFNFLKKELENFKKILQKENTQYLVKDFNENRCSIREAILDLTLYFLREMKQDEAADTLEDELIFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVASRRHEAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKKNREISFIFPLPFREMNLKEKETLSLMDLITQFFPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCCDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIERLTEIRGFNDAQKEEYFRKRLTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILQEKRNNGVKNNQADDASKTLQKSNTEDTPKTLTQMYTHFLRFQIQQSRRKYDGENTPDVSWDKDAILSLGKLAFHQLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVVGTMYCFVHLSVQEFIAALYAHLILDINKKSVFDQDSKNRNESMIDLLRTAVDKTLESDNGHLDLFLRFLLGLSLQSNRRLLQGLLTQQEHNDQSKEEIVQYIKQKFEVNHSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSADLSPAQWSALAFVLLTSEEEMEEFELQKFKKSDECLIRLSAVIKTCKRALLNDCNLTDRSCSTVAAVLRSENSLKELNMSNNNLQDSGVKLLCAGLKNIKCDLETLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSAAADEGCQFVTGIVGKNPLLLKELSLSGHELGDTRVNQLSALLQDKHCQINTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACEHLYKVLGGNLLLLRELNLTKHDLGPSGLEKLAAVLKDKHCKLNTLILNNSTISEEDFHVLTEAFNSNPTNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_017353263.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353263.1

ORF Insert Sequence:

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGAGTTA 
CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGTCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGC TTTGATTCCA 
GAGTGTGCTC TGAAAACATT GAGGTTTTTG GGTCCTGCAG CAGAAGAAGC CTGTCAGTTT 
GTGACTGGAA TTGTGGGTAA AAACCCGTTA CTCCTGAAAG AACTGAATCT GAGTGATCGT 
AAACTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGTAAA 
CCCAACACAC TTCAGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTAACT 
TCAGCATTTA ATTTAAACCC TTCACACCTA ATAGAGCTCA ATCTGAGTGG GAATAAACTA 
AGAGACTCAG GAGTAGAGGA GATCTGTCCT CTGCTGAAAA ATACACAGTG CAGACTGGAG 
AGACTAAGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTAA AGGAGCTCAC TGACTTACTG CAGGATGAGC GATATAGATT GAAGACCCTC 
AGGCTTTTGA AAACTACTGC AGCACAAGAA GCGTGTGACT ATCTTACTAA AGTTGTGGGT 
AAAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG ATAAATTAGG AGATCTGGAT 
GGAGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGTA AAGTGGAGAA AATCAAGCTG 
AATAATTGTA AGCAGATTGA AAAAGGCTGT TCAGTACTAG CTGCCGTTCT CTCCTCAAAA 
ACAATCCTAA AAGAGATGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCTTAGAG 
ATCTGTGAGG GACTGAAGAA CCCTGTGTGT GAGCTGAAGA TTCTCAAGCT CAGTAAGTGT 
GATTTAACAG AGGAAAGCTG TTCAGCTCTG GCTTCAGTTC TCAGATCAGA CTCCAGCAGT 
CTGAAGGATC TTGACCTGAG CAATAATAAT CTGCAGGATT CAGGAGTGAA GTTGCTCTCT 
GATGGACTAA AGCACAGTAA ACTGAAGAGA CTCAGTCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA CTTACTGCAG 
GATCCACACT GTACACTAAA GACACTGAGT TGTTTTAGTC CTGCTGCTGA TGAAGGCTGT 
CAGTTTGTTA CTGGAATTGT GGGTAAAAAC CCGTTACTAC TGAAAGAACT GAATCTGAGT 
GATCGTGAGC TAGGAGACAC ACGGGTGAAT CAACTCTCTG CTCTACTGCA AGATAAACAC 
TGTACACTCA ATATACTCAC GCTGAATAAT AACAGGATTA CAGCAGAAGG TTGTGCTGCT 
TTGACTTCAG CATTTAATTT AAACCCTTCA CACCTGATAG AGCTCAATCT GAGTGGAAAT 
AAACTAGGAG ACTCAGGAGT AGAGAAGATC TGTCCTCTGC TGAAAAATAC CCAGTGCAGA 
TTGGAGAAAT TAAATCTTTC AGACTGCAGC ATCACTGGAA AAGGTTATGA AACATTGATT 
TCAGCTTTGA AGTCAAACTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGG 
AAATCAGGAG TGCAGAAGCT CACTGATTTG CTACGAGCTG GAAGCTGTAA ATTAAAAACC 
ATCAGGACTT TGAAAGGTTC CGCTGCACAG GAAGCGTGTG ACTATCTAAC TAAAGCTGTG 
GGTAAAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG 
GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGGA GAAAATCAGG 
ATGAATAACT GTAAAAAGCT GACAGAGAAA AGCTGTTTAG TTCTAGCTGC TGTTCTTTCA 
TCCAAAACCA TCTTGAAAGA GATGAACCTA AACAACAGTC GTCTGCTGGA CTCAGGAGTC 
AAAGTGATCT GTGAGGGACT GAAGAACTCT AAGCTGGAGA TTTTAAAACT TTCAAATTGT 
AGTATTACAG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CTGGAGTAAA GGAGCTCACT 
GAGTTACTGC AGAGTTCGTG GATTCCATGG AAAAAAGTGA AGTGTTCAAT GAGGTTTTTG 
AGTCCTGCTG CTGATGAATT TTGTCAGTTT GTGTCTGGAA TTGTGGGTAA AAACCCGTTA 
CTCCTGAAAG AACTGAATCT GAGTGATCAT GTACTAGCAG ACACACAAGT GAATCAACTC 
TCTGCTCTAC TGCAGGATAA ACACTGCAAA CTCAACACAC TCATTCTTTC AGACTGCAGT 
ATCAGTGGAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAAGA GCTCACTGAG 
TTACTGCAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTGA TGATGCAGAT 
GAAGCCTGTG AACATTTGTA TAAAGTTCTG GGTGGAAACC TGTTACTCCT GAGAGAGCTG 
AATCTGACTA AACATGATCT TGGACCATCA GGATTAGAGA AACTTGCTGC AGTACTGAAA 
GATAAACACT GTAAACTCAA CACACTGATT CTGAATAACA GCACTATCTC AGAGGAAGAT 
TTTCATGTTC TGACTGAAGC TTTTAATTCA AACCCAACAA ATCTGACAGA GCTGAACTTG 
AGTGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA CAACTCTGTT TCAAAACCAA 
CAGTGTAGAT TGGAAAAGCT GAAGTTCAGC AGCATGTGTA TTACAGCAGA AGGTTGTGCT 
GCTTTGACTT CAGCATTTAA CTCAAACCCT TCACACCTGA AAGAGCTCAA TCTGAGTGGG 
AATAAACTAG GAGACTCAGG AGTAAGAAAA ATCTCCAGTC TACTGAGAAA CTCGCAGTGC 
ATGCTGAAGA TACTCGGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTTACAT CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACCAT CAGGAGTGAA GGAGCTCACT GGCTTACTAC TCGATCCACA CTGTACACTG 
ACGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAATTCT GTCAGTTTGT GTCTGGAATT 
GTGGGTAAAT ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGG ACTAGGGGAC 
ACACGAGTGA ATCAACTCTC TGCTCTACTG CAGGATAAAC ACTTTAGACT CAACACACTC 
ATGCTGAATA ATAACAGGAT TACAGCAGAA GGTTGTGCTG CTTTAACTTC AGCATTTAAT 
TTAAATCCTT CACACCTGAT AGAGCTCAAT CTGAGTGGGA ATAAACTAGA AGACTCAGGA 
GTAGAGAAGA TCTGTCCTCT GCTGGAAAAT ATACAATGCC GATTAGAGAA ACTGAGTCTT 
TCAGACTGCA GTATCAGGGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG ATTTACTGCA GAGTAGAAGC TGTAAATTCA GGTTTCTGAA GAGTTCTGCT 
GCACAGGAAG CATGCGAGTA TCTTACTAAT GTTCTGGGTA TAAGTCCATT ATTACTGACA 
GAACTGGATC TGAGTGAAGT TAAATTAGGA GATCTGGACT GGGAGAAACT CTCTGCTCTT 
CTGATGGACT CTCATAGCAA AGTGGAGAAA ATCAAGCTAA ATAATTGTGA GCTGACAGAG 
AAAAGCTGTT CAGTTCTAGC TGCTGTTCTC TCCTCCAAAA CCATCCTGAA AGAGCTGAAC 
CTGAACAACA GTCGTCTGCT GGACTCAGGA GTCAGAAAGA TCTGTGAGGG ACTGAAGAAT 
CCTGTGTGTG AGCTGAAGAT ACTCAATCTT TCAGACTGCA GTATTACAGA AGAAGGTTAT 
GAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTGCACTGA AGACACTGAG ATTTTTAAGA AGTCCTGCTG CTGATGAAGG CTGTCAGTTA 
GTAACTGGAG TTGTGGGTAA AAACCCGTTA CTCCTGAAGG AACTGAATCT GAGTGATCAT 
GAGCTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGCCAG 
ATCAACACAC TCAATCTGAG TTATTGCAGT ATTACAGAGA AACAGTGTGA GATCCTGACT 
TCAGCTCTGA AATCAAACCC ATCACACCTG AGAGAACTGG ACCTCAGTGG GAATGAACTA 
GGAGACTTTC AAAGCCTTGG CTCTCTACTG AAGAACCAAC AATTCAAGCT GGAGAAACTA 
ATTCTGTGTG TTTGCAGTAT TAAAGAGAAG CAGTGTGAGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGGA ATGAACTCAA GCACAGAAGA 
GTGAAGCAAT TTTTTGATGT GCTGAAAAAA TCAACCTGTA AACTGGAGCG ACTGAGGTTA 
AGATACTGTA GTATACAAGC CGATGATTGT ACTGATCTGG CTTCAGCTCT GAAATCAAAC 
CCATCCCACC TGAGAGAACT GGACCTCAGT GGAAACAATC TAGGAGACTC TGGAGTGAAA 
AAACTCAGTG ATTTACTGAT AAACCAACAA TTCAAGCTGG AGAAACTACG TCTGAGTTAT 
TGCAGTATTA CAGAGAAACA GTGTGTGATC CTGACTTCAG CTCTGAAATC AAACCCATCA 
CACCTGAGAG AACTGAACCT CAATGGGAAT AGACTAGGAG ACTCTGGAGC TGATGGCCTT 
AACCATCTGC TGAGCAGTGT GGATTGCAAG CTAAATAAAC TACATCTGTG TGATTGCAGT 
ATTACAGAGA AACAGTTGCT CATCCCAATC ACAGCTACGT GTATGGGCCT ATTACACCTG 
AGAGAACTGG ACCTCAGTAA GAACCAAATA AAAAACCAAG GAGTGTACAT GCTGTGTGAT 
GTGCTGAAAA ACCCACGCTG TAGACTCAAG AGGTTAAGAC TAAATGACTG TGGCATTACA 
GATGTTACAC CCTTAACTCA GTCTTTGACC AAGGACAAAG CATTGAAGTT CTTAAAAGAG 
CTTGAACTGA GTAAAAATCA AATGGGATTC TTCGAAAAGC AAAAACTCAG TGACCTGCTG 
CGAGACTCAA ACTGTAAACT GAGGCTTGAG GAAGAAGAAG GTTATTTTAG AGCTGGTGTA 
AAACGGGTTA CTACATTTTT GATGCCTCCA TGGTCAAAAG TTCAAAAACC TGTGGATCTC 
ACAGGAGAGG AAAGTTCATC ATCAGATGAG GACGCCTCTG AAGATGAGAC TGATCAGGCA 
GAGGAGGCTG ATAAGCTCTC AAATGAGGCG GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_017353264.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208753.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X5, mRNA.

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CloneID	ODa57135
Clone ID Related Accession (Same CDS sequence)	XM_017353264.1
Accession Version	XM_017353264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	protein NLRC5 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAACGGGGTG ATTGCTGTAA ATTAAAAACC GTCAGGTTTT TAAAGAGTTC TGCTGCACAG 
GAAGCGTGTG ACTATCTTAC TAAAGCTGTG GGTAAAAGTC CATTATTACT GACAAAACTG 
GATCTGAGTG AAGTTAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGAAG 
GACTCTCATA GCAAAGTGGA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCAGTTC TAGCTGCTGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAGATCTGTG AGGGGCTGAA GAATCCTGTG 
TGTGAGCTGA AGATACTCAA CCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACTTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGACTTAC TGCAGGATCC ACACTGTACC 
CTGAATACAC TGAGGTTTTT GAGTCCTGCC GCAGAAGAAG CCTGTCAGTT TGTGACTGGA 
ATTGTGGGTA AAAACCCGAT ACTTCTGAAA GAACTGAATC TGAGTGATCA TGAACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGTAA ACTCAACACA 
CTGATTCTGA AAAACAGCTT TATCTCAGAG GAAGATTGTC GTGTTCTGGC TGAAGCTTTT 
AATTCAAACC CATCAAATCT GAGAGAGCTG AATTTGAATG GGACTAAACT CACAAACTCA 
GGAATGAAGA GCTTCAACAC TCTGTTTCAA AACCAACAGT GCAGACTGGA AAAGCTGAAG 
TTTAGCGGCA TCAGTATCAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAGCTCA 
AACCCGTCAC ACCTGATAGA GCTAAATCTG AGTGGGAATA CACTAGAAGA CTCAGGAGTA 
ACAGAAATCT CCAGTCTACT GGCAAACTCA CAGTGCATGC TGAAGATACT CAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGCTT TGATTCCAGA GTGTGCTCTG AAAACATTGA GGTTTTTGGG TCCTGCAGCA 
GAAGAAGCCT GTCAGTTTGT GACTGGAATT GTGGGTAAAA ACCCGTTACT CCTGAAAGAA 
CTGAATCTGA GTGATCGTAA ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTACTG 
CAGGATAAAC ACTGTAAACC CAACACACTT CAGCTGAATA ATAACAGGAT TACAGCAGAA 
GGTTGTGCTG CTTTAACTTC AGCATTTAAT TTAAACCCTT CACACCTAAT AGAGCTCAAT 
CTGAGTGGGA ATAAACTAAG AGACTCAGGA GTAGAGGAGA TCTGTCCTCT GCTGAAAAAT 
ACACAGTGCA GACTGGAGAG ACTAAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTAAAG GAGCTCACTG ACTTACTGCA GGATGAGCGA 
TATAGATTGA AGACCCTCAG GCTTTTGAAA ACTACTGCAG CACAAGAAGC GTGTGACTAT 
CTTACTAAAG TTGTGGGTAA AAGTCCATTA TTACTGACAG AACTGGATCT GAGTGAAGAT 
AAATTAGGAG ATCTGGATGG AGAGAAACTC TCTGCTCTTC TGATGGACTC TCATAGTAAA 
GTGGAGAAAA TCAAGCTGAA TAATTGTAAG CAGATTGAAA AAGGCTGTTC AGTACTAGCT 
GCCGTTCTCT CCTCAAAAAC AATCCTAAAA GAGATGAACC TGAACAACAG TCGTCTGCTG 
GACTCAGGAG TCTTAGAGAT CTGTGAGGGA CTGAAGAACC CTGTGTGTGA GCTGAAGATT 
CTCAAGCTCA GTAAGTGTGA TTTAACAGAG GAAAGCTGTT CAGCTCTGGC TTCAGTTCTC 
AGATCAGACT CCAGCAGTCT GAAGGATCTT GACCTGAGCA ATAATAATCT GCAGGATTCA 
GGAGTGAAGT TGCTCTCTGA TGGACTAAAG CACAGTAAAC TGAAGAGACT CAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGACT TACTGCAGGA TCCACACTGT ACACTAAAGA CACTGAGTTG TTTTAGTCCT 
GCTGCTGATG AAGGCTGTCA GTTTGTTACT GGAATTGTGG GTAAAAACCC GTTACTACTG 
AAAGAACTGA ATCTGAGTGA TCGTGAGCTA GGAGACACAC GGGTGAATCA ACTCTCTGCT 
CTACTGCAAG ATAAACACTG TACACTCAAT ATACTCACGC TGAATAATAA CAGGATTACA 
GCAGAAGGTT GTGCTGCTTT GACTTCAGCA TTTAATTTAA ACCCTTCACA CCTGATAGAG 
CTCAATCTGA GTGGAAATAA ACTAGGAGAC TCAGGAGTAG AGAAGATCTG TCCTCTGCTG 
AAAAATACCC AGTGCAGATT GGAGAAATTA AATCTTTCAG ACTGCAGCAT CACTGGAAAA 
GGTTATGAAA CATTGATTTC AGCTTTGAAG TCAAACTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGGAA ATCAGGAGTG CAGAAGCTCA CTGATTTGCT ACGAGCTGGA 
AGCTGTAAAT TAAAAACCAT CAGGACTTTG AAAGGTTCCG CTGCACAGGA AGCGTGTGAC 
TATCTAACTA AAGCTGTGGG TAAAAGTCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA CTCTCTGCTC TTCTGATGGA CTCTCATAGC 
AAAGTGGAGA AAATCAGGAT GAATAACTGT AAAAAGCTGA CAGAGAAAAG CTGTTTAGTT 
CTAGCTGCTG TTCTTTCATC CAAAACCATC TTGAAAGAGA TGAACCTAAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAA AGTGATCTGT GAGGGACTGA AGAACTCTAA GCTGGAGATT 
TTAAAACTTT CAAATTGTAG TATTACAGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCT 
GGAGTAAAGG AGCTCACTGA GTTACTGCAG AGTTCGTGGA TTCCATGGAA AAAAGTGAAG 
TGTTCAATGA GGTTTTTGAG TCCTGCTGCT GATGAATTTT GTCAGTTTGT GTCTGGAATT 
GTGGGTAAAA ACCCGTTACT CCTGAAAGAA CTGAATCTGA GTGATCATGT ACTAGCAGAC 
ACACAAGTGA ATCAACTCTC TGCTCTACTG CAGGATAAAC ACTGCAAACT CAACACACTC 
ATGCTGAATA ATAACAGGAT TACAGCAGAA GGTTGTGCTG CTTTAACTTC AGCATTTAAT 
TTAAATCCTT CACACCTGAT AGAGCTCAAT CTGAGTGGGA ATAAACTAGA AGACTCAGGA 
GTAGAGAAGA TCTGTCCTCT GCTGGAAAAT ATACAATGCC GATTAGAGAA ACTGAGTCTT 
TCAGACTGCA GTATCAGGGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG ATTTACTGCA GAGTAGAAGC TGTAAATTCA GGTTTCTGAA GAGTTCTGCT 
GCACAGGAAG CATGCGAGTA TCTTACTAAT GTTCTGGGTA TAAGTCCATT ATTACTGACA 
GAACTGGATC TGAGTGAAGT TAAATTAGGA GATCTGGACT GGGAGAAACT CTCTGCTCTT 
CTGATGGACT CTCATAGCAA AGTGGAGAAA ATCAAGCTAA ATAATTGTGA GCTGACAGAG 
AAAAGCTGTT CAGTTCTAGC TGCTGTTCTC TCCTCCAAAA CCATCCTGAA AGAGCTGAAC 
CTGAACAACA GTCGTCTGCT GGACTCAGGA GTCAGAAAGA TCTGTGAGGG ACTGAAGAAT 
CCTGTGTGTG AGCTGAAGAT ACTCAATCTT TCAGACTGCA GTATTACAGA AGAAGGTTAT 
GAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTGCACTGA AGACACTGAG ATTTTTAAGA AGTCCTGCTG CTGATGAAGG CTGTCAGTTA 
GTAACTGGAG TTGTGGGTAA AAACCCGTTA CTCCTGAAGG AACTGAATCT GAGTGATCAT 
GAGCTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGCCAG 
ATCAACACAC TCAATCTGAG TTATTGCAGT ATTACAGAGA AACAGTGTGA GATCCTGACT 
TCAGCTCTGA AATCAAACCC ATCACACCTG AGAGAACTGG ACCTCAGTGG GAATGAACTA 
GGAGACTTTC AAAGCCTTGG CTCTCTACTG AAGAACCAAC AATTCAAGCT GGAGAAACTA 
ATTCTGTGTG TTTGCAGTAT TAAAGAGAAG CAGTGTGAGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGGA ATGAACTCAA GCACAGAAGA 
GTGAAGCAAT TTTTTGATGT GCTGAAAAAA TCAACCTGTA AACTGGAGCG ACTGAGGTTA 
AGATACTGTA GTATACAAGC CGATGATTGT ACTGATCTGG CTTCAGCTCT GAAATCAAAC 
CCATCCCACC TGAGAGAACT GGACCTCAGT GGAAACAATC TAGGAGACTC TGGAGTGAAA 
AAACTCAGTG ATTTACTGAT AAACCAACAA TTCAAGCTGG AGAAACTACG TCTGAGTTAT 
TGCAGTATTA CAGAGAAACA GTGTGTGATC CTGACTTCAG CTCTGAAATC AAACCCATCA 
CACCTGAGAG AACTGAACCT CAATGGGAAT AGACTAGGAG ACTCTGGAGC TGATGGCCTT 
AACCATCTGC TGAGCAGTGT GGATTGCAAG CTAAATAAAC TACATCTGTG TGATTGCAGT 
ATTACAGAGA AACAGTTGCT CATCCCAATC ACAGCTACGT GTATGGGCCT ATTACACCTG 
AGAGAACTGG ACCTCAGTAA GAACCAAATA AAAAACCAAG GAGTGTACAT GCTGTGTGAT 
GTGCTGAAAA ACCCACGCTG TAGACTCAAG AGGTTAAGAC TAAATGACTG TGGCATTACA 
GATGTTACAC CCTTAACTCA GTCTTTGACC AAGGACAAAG CATTGAAGTT CTTAAAAGAG 
CTTGAACTGA GTAAAAATCA AATGGGATTC TTCGAAAAGC AAAAACTCAG TGACCTGCTG 
CGAGACTCAA ACTGTAAACT GAGGCTTGAG GAAGAAGAAG GTTATTTTAG AGCTGGTGTA 
AAACGGGTTA CTACATTTTT GATGCCTCCA TGGTCAAAAG TTCAAAAACC TGTGGATCTC 
ACAGGAGAGG AAAGTTCATC ATCAGATGAG GACGCCTCTG AAGATGAGAC TGATCAGGCA 
GAGGAGGCTG ATAAGCTCTC AAATGAGGCG GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208753.1
CDS	188..9163
Translation	MKMSLSEKHSVRSGSHVSSSVSVKSDRSKGHMPDFSETKTSPNKRFQYKTLEFDFQPQRSCNNFKDLQCIFQGLESKLFNFLKKELENFKKILQKENTQYLVKDFNENRCSIREAILDLTLYFLREMKQDEAADTLEDELIFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVASRRHEAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKKNREISFIFPLPFREMNLKEKETLSLMDLITQFFPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCCDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIERLTEIRGFNDAQKEEYFRKRLTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILQEKRNNGVKNNQADDASKTLQKSNTEDTPKTLTQMYTHFLRFQIQQSRRKYDGENTPDVSWDKDAILSLGKLAFHQLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVVGTMYCFVHLSVQEFIAALYAHLILDINKKSVFDQDSKNRNESMIDLLRTAVDKTLESDNGHLDLFLRFLLGLSLQSNRRLLQGLLTQQEHNDQSKEEIVQYIKQKFEVNHSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSADLSPAQWSALAFVLLTSEEEMEEFELQKFKKSDECLIRLSAVIKTCKRALLNDCNLTDRSCSTVAAVLRSENSLKELNMSNNNLQDSGVKLLCAGLKNIKCDLETLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSAAADEGCQFVTGIVGKNPLLLKELSLSGHELGDTRVNQLSALLQDKHCQINTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLKFSGISITAEGCAALTSAFSSNPSHLIELNLSGNTLEDSGVTEISSLLANSQCMLKILSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_017353264.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353264.1

ORF Insert Sequence:

ATGAAGATGA GTCTCTCAGA GAAACACAGT GTAAGATCAG GCTCACATGT GTCCAGCTCT 
GTGTCTGTAA AGAGTGACCG GTCAAAAGGT CACATGCCGG ACTTCAGTGA GACAAAAACC 
TCACCAAATA AAAGGTTTCA ATATAAGACA TTAGAATTTG ACTTCCAACC TCAAAGGAGC 
TGCAATAATT TCAAAGACCT CCAGTGCATC TTCCAGGGGC TTGAGAGCAA ACTGTTCAAC 
TTTCTGAAGA AAGAGTTGGA AAATTTTAAG AAAATCTTAC AAAAAGAAAA CACACAATAC 
CTTGTAAAGG ACTTTAATGA AAACAGATGC AGTATCAGAG AAGCAATACT TGATCTCACA 
CTCTACTTCC TGAGAGAAAT GAAGCAAGAT GAAGCTGCTG ATACTCTAGA AGATGAGCTG 
ATCTTCATTC ATCAGCTAAA ATGTGGCCTA CAGAAGAAGT ATCAGTGTGT GTTTGAAGGA 
ATTGCACAGC AAGGAGACTC CACACTCCTG AAGAACATTT ACACAGATCT CTATATCACT 
CAGGGTGGCA GTGAACAGGT CAATACTGAA CATGAGGTCA GACAGATTGA AGTTGCTTCC 
AGACGTCATG AAGCTCAAGA GATACAGGTT GAATGCAGAC ATTTGTTTGA AGCATCTGAA 
CAAGACAAGC AGATCAGAAC TGTACTGACA AAAGGAGTCG CTGGCATCGG GAAATCAGTC 
TCTGTGCAAA AGTTTGTTCT GGATTGGGCT GAAGGAAAAA AAAATCGAGA GATCAGCTTC 
ATATTTCCTC TTCCATTCAG AGAGATGAAC TTAAAGGAGA AAGAAACACT CAGTTTGATG 
GACCTTATAA CTCAGTTTTT CCCAGAGACA AAAGGACTGA ACCTTACAAG AAGGAATCGA 
TTCAAAGTCC TGTTCATCCT TGATGGATTG GATGAATGTC GTCTTCCTCT GAACTTTGCT 
GGTAATGAGA CGTGTTGTGA CGTTTCATCA CCAGCCTCTC TGGATGTTCT CCTGACGAAC 
CTCATCAAGG GAAATCTGCT TCCTTCTGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCT 
GCCAGTAAGA TTCCTCCTGA CTGTATCGAG CGGCTGACAG AGATACGAGG ATTCAATGAT 
GCACAAAAGG AGGAGTACTT CAGAAAAAGA CTCACAGATG AGAATCAGGC CAAAGAAATC 
ATTGATCATG TTAAACAATC AAAGAGTCTC TTCATCATGT GCCACATCCC AGTCTTCTGC 
TGGATTTCAG CCACTGTTCT CCAGAACATT TTACAGGAGA AGAGAAATAA TGGTGTGAAA 
AACAATCAGG CTGATGACGC CTCCAAAACA CTGCAGAAAT CAAACACTGA AGACACTCCC 
AAGACTCTGA CACAGATGTA CACACACTTT CTGCGCTTTC AGATCCAGCA GAGCAGACGA 
AAGTATGATG GAGAAAACAC ACCAGATGTT TCCTGGGATA AAGATGCCAT CCTTTCACTG 
GGGAAACTGG CATTTCATCA GCTGCTAAAC AGCAACCTGA TCTTCTATGA CACAGACCTG 
GAAGCCTGTG GTATTGACGT CTATAAGGCC TCAGTTTACT CAGGCATGTG TACCCAGATC 
TTTAAGGAGG AAACAGGGAT TGTTGTTGGT ACCATGTACT GCTTTGTTCA CTTGAGCGTT 
CAAGAGTTTA TTGCAGCCCT TTATGCACAT CTGATTCTAG ACATCAACAA GAAAAGTGTG 
TTTGATCAAG ACTCTAAAAA CAGAAATGAG TCCATGATTG ATTTGCTGAG GACTGCAGTG 
GACAAGACAC TTGAGAGTGA CAATGGACAC CTGGACCTTT TCCTTCGCTT CCTTCTCGGT 
CTGTCACTCC AGTCCAATCG CCGACTCTTA CAAGGTCTGT TGACACAGCA AGAACACAAT 
GACCAGAGCA AAGAGGAAAT AGTTCAGTAC ATCAAGCAGA AGTTTGAGGT GAATCATTCT 
CCAGAGAGAT CCATCAATCT GTTCTACTGT CTGAATGAAC TGAACGACCA AACTCTGGTG 
AAAGAGATTC AGACTCACCT TAGCAAAGGA AGTCTCTCAT CTGCTGATCT TTCACCTGCC 
CAATGGTCTG CTTTGGCCTT CGTGTTGTTG ACATCAGAGG AGGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGAC TATCAGCAGT CATCAAAACC 
TGCAAAAGAG CTCTGTTAAA TGATTGTAAC TTAACAGACA GAAGCTGTTC AACTGTGGCT 
GCGGTTCTCA GATCAGAAAA CAGTCTGAAA GAGCTGAACA TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTGTGCTGGA CTGAAGAATA TTAAGTGCGA TTTAGAAACG 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTAAA GACACTGAGG 
TTTTTGAGTG CTGCTGCTGA TGAAGGCTGT CAGTTTGTGA CTGGAATTGT GGGTAAAAAC 
CCTTTACTTC TGAAAGAACT GAGTCTAAGT GGTCACGAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTCAGATCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTCAG CATTTAATTT AAATCCATCA 
AACCTGATGG AGCTCACTCT GAGTGGAAAT AAACTAGAAG ACTCAGGAGT AGAAAAGATC 
TGTTCTCTGC TGAAAAATAC ACAGTGCAGA TTGAAGAAAC TGAGTCTTTC AGACTGCAGG 
ATCACTGAAG AAGGATATAA AAGTTTGACT TTGTCCCTGA AGTCAAATTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAACGGGGTG ATTGCTGTAA ATTAAAAACC GTCAGGTTTT TAAAGAGTTC TGCTGCACAG 
GAAGCGTGTG ACTATCTTAC TAAAGCTGTG GGTAAAAGTC CATTATTACT GACAAAACTG 
GATCTGAGTG AAGTTAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGAAG 
GACTCTCATA GCAAAGTGGA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCAGTTC TAGCTGCTGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAGATCTGTG AGGGGCTGAA GAATCCTGTG 
TGTGAGCTGA AGATACTCAA CCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACTTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGACTTAC TGCAGGATCC ACACTGTACC 
CTGAATACAC TGAGGTTTTT GAGTCCTGCC GCAGAAGAAG CCTGTCAGTT TGTGACTGGA 
ATTGTGGGTA AAAACCCGAT ACTTCTGAAA GAACTGAATC TGAGTGATCA TGAACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGTAA ACTCAACACA 
CTGATTCTGA AAAACAGCTT TATCTCAGAG GAAGATTGTC GTGTTCTGGC TGAAGCTTTT 
AATTCAAACC CATCAAATCT GAGAGAGCTG AATTTGAATG GGACTAAACT CACAAACTCA 
GGAATGAAGA GCTTCAACAC TCTGTTTCAA AACCAACAGT GCAGACTGGA AAAGCTGAAG 
TTTAGCGGCA TCAGTATCAC AGCAGAAGGT TGTGCTGCTT TGACTTCAGC ATTTAGCTCA 
AACCCGTCAC ACCTGATAGA GCTAAATCTG AGTGGGAATA CACTAGAAGA CTCAGGAGTA 
ACAGAAATCT CCAGTCTACT GGCAAACTCA CAGTGCATGC TGAAGATACT CAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGCTT TGATTCCAGA GTGTGCTCTG AAAACATTGA GGTTTTTGGG TCCTGCAGCA 
GAAGAAGCCT GTCAGTTTGT GACTGGAATT GTGGGTAAAA ACCCGTTACT CCTGAAAGAA 
CTGAATCTGA GTGATCGTAA ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTACTG 
CAGGATAAAC ACTGTAAACC CAACACACTT CAGCTGAATA ATAACAGGAT TACAGCAGAA 
GGTTGTGCTG CTTTAACTTC AGCATTTAAT TTAAACCCTT CACACCTAAT AGAGCTCAAT 
CTGAGTGGGA ATAAACTAAG AGACTCAGGA GTAGAGGAGA TCTGTCCTCT GCTGAAAAAT 
ACACAGTGCA GACTGGAGAG ACTAAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTAAAG GAGCTCACTG ACTTACTGCA GGATGAGCGA 
TATAGATTGA AGACCCTCAG GCTTTTGAAA ACTACTGCAG CACAAGAAGC GTGTGACTAT 
CTTACTAAAG TTGTGGGTAA AAGTCCATTA TTACTGACAG AACTGGATCT GAGTGAAGAT 
AAATTAGGAG ATCTGGATGG AGAGAAACTC TCTGCTCTTC TGATGGACTC TCATAGTAAA 
GTGGAGAAAA TCAAGCTGAA TAATTGTAAG CAGATTGAAA AAGGCTGTTC AGTACTAGCT 
GCCGTTCTCT CCTCAAAAAC AATCCTAAAA GAGATGAACC TGAACAACAG TCGTCTGCTG 
GACTCAGGAG TCTTAGAGAT CTGTGAGGGA CTGAAGAACC CTGTGTGTGA GCTGAAGATT 
CTCAAGCTCA GTAAGTGTGA TTTAACAGAG GAAAGCTGTT CAGCTCTGGC TTCAGTTCTC 
AGATCAGACT CCAGCAGTCT GAAGGATCTT GACCTGAGCA ATAATAATCT GCAGGATTCA 
GGAGTGAAGT TGCTCTCTGA TGGACTAAAG CACAGTAAAC TGAAGAGACT CAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGACT TACTGCAGGA TCCACACTGT ACACTAAAGA CACTGAGTTG TTTTAGTCCT 
GCTGCTGATG AAGGCTGTCA GTTTGTTACT GGAATTGTGG GTAAAAACCC GTTACTACTG 
AAAGAACTGA ATCTGAGTGA TCGTGAGCTA GGAGACACAC GGGTGAATCA ACTCTCTGCT 
CTACTGCAAG ATAAACACTG TACACTCAAT ATACTCACGC TGAATAATAA CAGGATTACA 
GCAGAAGGTT GTGCTGCTTT GACTTCAGCA TTTAATTTAA ACCCTTCACA CCTGATAGAG 
CTCAATCTGA GTGGAAATAA ACTAGGAGAC TCAGGAGTAG AGAAGATCTG TCCTCTGCTG 
AAAAATACCC AGTGCAGATT GGAGAAATTA AATCTTTCAG ACTGCAGCAT CACTGGAAAA 
GGTTATGAAA CATTGATTTC AGCTTTGAAG TCAAACTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGGAA ATCAGGAGTG CAGAAGCTCA CTGATTTGCT ACGAGCTGGA 
AGCTGTAAAT TAAAAACCAT CAGGACTTTG AAAGGTTCCG CTGCACAGGA AGCGTGTGAC 
TATCTAACTA AAGCTGTGGG TAAAAGTCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA CTCTCTGCTC TTCTGATGGA CTCTCATAGC 
AAAGTGGAGA AAATCAGGAT GAATAACTGT AAAAAGCTGA CAGAGAAAAG CTGTTTAGTT 
CTAGCTGCTG TTCTTTCATC CAAAACCATC TTGAAAGAGA TGAACCTAAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAA AGTGATCTGT GAGGGACTGA AGAACTCTAA GCTGGAGATT 
TTAAAACTTT CAAATTGTAG TATTACAGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCT 
GGAGTAAAGG AGCTCACTGA GTTACTGCAG AGTTCGTGGA TTCCATGGAA AAAAGTGAAG 
TGTTCAATGA GGTTTTTGAG TCCTGCTGCT GATGAATTTT GTCAGTTTGT GTCTGGAATT 
GTGGGTAAAA ACCCGTTACT CCTGAAAGAA CTGAATCTGA GTGATCATGT ACTAGCAGAC 
ACACAAGTGA ATCAACTCTC TGCTCTACTG CAGGATAAAC ACTGCAAACT CAACACACTC 
ATGCTGAATA ATAACAGGAT TACAGCAGAA GGTTGTGCTG CTTTAACTTC AGCATTTAAT 
TTAAATCCTT CACACCTGAT AGAGCTCAAT CTGAGTGGGA ATAAACTAGA AGACTCAGGA 
GTAGAGAAGA TCTGTCCTCT GCTGGAAAAT ATACAATGCC GATTAGAGAA ACTGAGTCTT 
TCAGACTGCA GTATCAGGGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG ATTTACTGCA GAGTAGAAGC TGTAAATTCA GGTTTCTGAA GAGTTCTGCT 
GCACAGGAAG CATGCGAGTA TCTTACTAAT GTTCTGGGTA TAAGTCCATT ATTACTGACA 
GAACTGGATC TGAGTGAAGT TAAATTAGGA GATCTGGACT GGGAGAAACT CTCTGCTCTT 
CTGATGGACT CTCATAGCAA AGTGGAGAAA ATCAAGCTAA ATAATTGTGA GCTGACAGAG 
AAAAGCTGTT CAGTTCTAGC TGCTGTTCTC TCCTCCAAAA CCATCCTGAA AGAGCTGAAC 
CTGAACAACA GTCGTCTGCT GGACTCAGGA GTCAGAAAGA TCTGTGAGGG ACTGAAGAAT 
CCTGTGTGTG AGCTGAAGAT ACTCAATCTT TCAGACTGCA GTATTACAGA AGAAGGTTAT 
GAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG AGTTACTGCA GGATCCACAC 
TGTGCACTGA AGACACTGAG ATTTTTAAGA AGTCCTGCTG CTGATGAAGG CTGTCAGTTA 
GTAACTGGAG TTGTGGGTAA AAACCCGTTA CTCCTGAAGG AACTGAATCT GAGTGATCAT 
GAGCTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGCCAG 
ATCAACACAC TCAATCTGAG TTATTGCAGT ATTACAGAGA AACAGTGTGA GATCCTGACT 
TCAGCTCTGA AATCAAACCC ATCACACCTG AGAGAACTGG ACCTCAGTGG GAATGAACTA 
GGAGACTTTC AAAGCCTTGG CTCTCTACTG AAGAACCAAC AATTCAAGCT GGAGAAACTA 
ATTCTGTGTG TTTGCAGTAT TAAAGAGAAG CAGTGTGAGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGGA ATGAACTCAA GCACAGAAGA 
GTGAAGCAAT TTTTTGATGT GCTGAAAAAA TCAACCTGTA AACTGGAGCG ACTGAGGTTA 
AGATACTGTA GTATACAAGC CGATGATTGT ACTGATCTGG CTTCAGCTCT GAAATCAAAC 
CCATCCCACC TGAGAGAACT GGACCTCAGT GGAAACAATC TAGGAGACTC TGGAGTGAAA 
AAACTCAGTG ATTTACTGAT AAACCAACAA TTCAAGCTGG AGAAACTACG TCTGAGTTAT 
TGCAGTATTA CAGAGAAACA GTGTGTGATC CTGACTTCAG CTCTGAAATC AAACCCATCA 
CACCTGAGAG AACTGAACCT CAATGGGAAT AGACTAGGAG ACTCTGGAGC TGATGGCCTT 
AACCATCTGC TGAGCAGTGT GGATTGCAAG CTAAATAAAC TACATCTGTG TGATTGCAGT 
ATTACAGAGA AACAGTTGCT CATCCCAATC ACAGCTACGT GTATGGGCCT ATTACACCTG 
AGAGAACTGG ACCTCAGTAA GAACCAAATA AAAAACCAAG GAGTGTACAT GCTGTGTGAT 
GTGCTGAAAA ACCCACGCTG TAGACTCAAG AGGTTAAGAC TAAATGACTG TGGCATTACA 
GATGTTACAC CCTTAACTCA GTCTTTGACC AAGGACAAAG CATTGAAGTT CTTAAAAGAG 
CTTGAACTGA GTAAAAATCA AATGGGATTC TTCGAAAAGC AAAAACTCAG TGACCTGCTG 
CGAGACTCAA ACTGTAAACT GAGGCTTGAG GAAGAAGAAG GTTATTTTAG AGCTGGTGTA 
AAACGGGTTA CTACATTTTT GATGCCTCCA TGGTCAAAAG TTCAAAAACC TGTGGATCTC 
ACAGGAGAGG AAAGTTCATC ATCAGATGAG GACGCCTCTG AAGATGAGAC TGATCAGGCA 
GAGGAGGCTG ATAAGCTCTC AAATGAGGCG GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-11P18.6 ( XM_017353265.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208754.1 Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X6, mRNA.

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CloneID	ODa57136
Clone ID Related Accession (Same CDS sequence)	XM_017353265.1
Accession Version	XM_017353265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein si:ch211-11p18.6 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATA ATAATAATTT AAAGGATTCA GGAGTGAAGC TGCTTTGCAC TGGACTGAAG 
AACATAAAGT GTGAATTGGA AATACTGAGT CTTTCAGACT GCAATATCAG TGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGAGATCA AACCCTTCAC ACCTGATAGA GCTGAATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAGCTCA CTGAGTTACT GCAGGATCCA 
CACTGTACAC TGAAGACACT GAGGTTTTTG AGTCCTGCAG CAGAAGAATT CTGTCAATTT 
GTGACTGGAA TTGTGGGTAA AAACCCGTTA CTCCTGAAAG AACTGTATCT GAGTGATCAT 
GAACTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGTACA 
GTCAGCACAC TCATTCTGCA TAATTGTGGT CTAACAAAGG AAAGCTGTTC AGCTCTGGCT 
TCAGCCTTGA GTTCAGACAC CAGTCTAAAA GAGCTCGACC TGTGCAACAA TAATCTGCAG 
GATTCAGGAG TGAAGAAACT CCAGAGCGAA TTACAAAATA CAAACTGGAG ACTGAAGAAA 
CTCAGGCTCA GTAAGTGTGA TCTAACAGAG GAAAGCTGTT CAGCTCTTGC TTCAGTTCTC 
ACATCAGACT CCAGCAGTCT GAAGGATCTT GACCTGAGCA ATAATAATCT GCAGGATTCA 
GGAGTGAAGC TGCTCTCTGA TGGACTGAAG GACAGTAAAC TGGAGAAACT CAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTAAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTGATAGAGT TACTGCAGGA TCCACTCTGT ACACTGAAGA CACTGAGGTT TTTGAGTCCT 
GCTGCTGATG AAGCCTGTCA GTTTGTGACT GGAATTGTGG GTAAAAACCT GTTACTCCTG 
AAAGAACTGA ATCTGAGTGA TCGTCAACTA GGAGACACAC GAGTGAATCA ACTCTCTGCT 
CTACTGCAGG ATAAACACTG TACACTCAAC ACACTCATGC TGAATAATAA CAGGATTACA 
GCAGAAGGTT GTGCTGCTTT GACTTCAGCA TTTAATTTAA ATCCATCAAA CCTGATGGAG 
CTCACTCTGA GTGGAAATAA ACTAGAAGAC TCAGGAGTAG AAAAGATCTG TTCTCTGCTG 
AAAAATACAC AGTGCAGATT GAAGAAACTG AGTCTTTCAG ACTGCAGGAT CACTGAAGAA 
GGATATAAAA GTTTGACTTT GTCCCTGAAG TCAAATTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAGCTCA CTGATTTACA ACGGGGTGAT 
TGCTGTAAAT TAAAAACCGT CAGGTTTTTA AAGAGTTCTG CTGCACAGGA AGCGTGTGAC 
TATCTTACTA AAGCTGTGGG TAAAAGTCCA TTATTACTGA CAAAACTGGA TCTGAGTGAA 
GTTAAATTAG GAGATCTGGA TGGAGAGAAA CTCTCTGCTC TTCTGAAGGA CTCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT GAGCTGACAG AGAAAAGCTG TTCAGTTCTA 
GCTGCTGTTC TCTCCTCCAA AACCATCCTG AAAGAGATGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGGCTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAACC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CTTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGAAGCTCAC TGACTTACTG CAGGATCCAC ACTGTACCCT GAATACACTG 
AGGTTTTTGA GTCCTGCCGC AGAAGAAGCC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCGATAC TTCTGAAAGA ACTGAATCTG AGTGATCATG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT GATTCTGAAA 
AACAGCTTTA TCTCAGAGGA AGATTGTCGT GTTCTGGCTG AAGCTTTTAA TTCAAACCCA 
TCAAATCTGA GAGAGCTGAA TTTGAATGGG ACTAAACTCA CAAACTCAGG AATGAAGAGC 
TTCAACACTC TGTTTCAAAA CCAACAGTGC AGACTGGAAA AGCTGAAGTT TAGCGGCATC 
AGTATCACAG CAGAAGGTTG TGCTGCTTTG ACTTCAGCAT TTAGCTCAAA CCCGTCACAC 
CTGATAGAGC TAAATCTGAG TGGGAATACA CTAGAAGACT CAGGAGTAAC AGAAATCTCC 
AGTCTACTGG CAAACTCACA GTGCATGCTG AAGATACTCA GTCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGCTTTG 
ATTCCAGAGT GTGCTCTGAA AACATTGAGG TTTTTGGGTC CTGCAGCAGA AGAAGCCTGT 
CAGTTTGTGA CTGGAATTGT GGGTAAAAAC CCGTTACTCC TGAAAGAACT GAATCTGAGT 
GATCGTAAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGTAAACCCA ACACACTTCA GCTGAATAAT AACAGGATTA CAGCAGAAGG TTGTGCTGCT 
TTAACTTCAG CATTTAATTT AAACCCTTCA CACCTAATAG AGCTCAATCT GAGTGGGAAT 
AAACTAAGAG ACTCAGGAGT AGAGGAGATC TGTCCTCTGC TGAAAAATAC ACAGTGCAGA 
CTGGAGAGAC TAAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTAAAGGA GCTCACTGAC TTACTGCAGG ATGAGCGATA TAGATTGAAG 
ACCCTCAGGC TTTTGAAAAC TACTGCAGCA CAAGAAGCGT GTGACTATCT TACTAAAGTT 
GTGGGTAAAA GTCCATTATT ACTGACAGAA CTGGATCTGA GTGAAGATAA ATTAGGAGAT 
CTGGATGGAG AGAAACTCTC TGCTCTTCTG ATGGACTCTC ATAGTAAAGT GGAGAAAATC 
AAGCTGAATA ATTGTAAGCA GATTGAAAAA GGCTGTTCAG TACTAGCTGC CGTTCTCTCC 
TCAAAAACAA TCCTAAAAGA GATGAACCTG AACAACAGTC GTCTGCTGGA CTCAGGAGTC 
TTAGAGATCT GTGAGGGACT GAAGAACCCT GTGTGTGAGC TGAAGATTCT CAAGCTCAGT 
AAGTGTGATT TAACAGAGGA AAGCTGTTCA GCTCTGGCTT CAGTTCTCAG ATCAGACTCC 
AGCAGTCTGA AGGATCTTGA CCTGAGCAAT AATAATCTGC AGGATTCAGG AGTGAAGTTG 
CTCTCTGATG GACTAAAGCA CAGTAAACTG AAGAGACTCA GTCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGACTTA 
CTGCAGGATC CACACTGTAC ACTAAAGACA CTGAGTTGTT TTAGTCCTGC TGCTGATGAA 
GGCTGTCAGT TTGTTACTGG AATTGTGGGT AAAAACCCGT TACTACTGAA AGAACTGAAT 
CTGAGTGATC GTGAGCTAGG AGACACACGG GTGAATCAAC TCTCTGCTCT ACTGCAAGAT 
AAACACTGTA CACTCAATAT ACTCACGCTG AATAATAACA GGATTACAGC AGAAGGTTGT 
GCTGCTTTGA CTTCAGCATT TAATTTAAAC CCTTCACACC TGATAGAGCT CAATCTGAGT 
GGAAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGAA AAATACCCAG 
TGCAGATTGG AGAAATTAAA TCTTTCAGAC TGCAGCATCA CTGGAAAAGG TTATGAAACA 
TTGATTTCAG CTTTGAAGTC AAACTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGGAAAT CAGGAGTGCA GAAGCTCACT GATTTGCTAC GAGCTGGAAG CTGTAAATTA 
AAAACCATCA GGACTTTGAA AGGTTCCGCT GCACAGGAAG CGTGTGACTA TCTAACTAAA 
GCTGTGGGTA AAAGTCCATT ATTACTGACA GAACTGGATC TGAGTGAAGA TAAATTAGGA 
GATCTGGATG GAGAGAAACT CTCTGCTCTT CTGATGGACT CTCATAGCAA AGTGGAGAAA 
ATCAGGATGA ATAACTGTAA AAAGCTGACA GAGAAAAGCT GTTTAGTTCT AGCTGCTGTT 
CTTTCATCCA AAACCATCTT GAAAGAGATG AACCTAAACA ACAGTCGTCT GCTGGACTCA 
GGAGTCAAAG TGATCTGTGA GGGACTGAAG AACTCTAAGC TGGAGATTTT AAAACTTTCA 
AATTGTAGTA TTACAGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCTGG AGTAAAGGAG 
CTCACTGAGT TACTGCAGAG TTCGTGGATT CCATGGAAAA AAGTGAAGTG TTCAATGAGG 
TTTTTGAGTC CTGCTGCTGA TGAATTTTGT CAGTTTGTGT CTGGAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCATGTAC TAGCAGACAC ACAAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGCAAACTCA ACACACTCAT TCTTTCAGAC 
TGCAGTATCA GTGGAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAAGAGCTC 
ACTGAGTTAC TGCAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTGATGAT 
GCAGATGAAG CCTGTGAACA TTTGTATAAA GTTCTGGGTG GAAACCTGTT ACTCCTGAGA 
GAGCTGAATC TGACTAAACA TGATCTTGGA CCATCAGGAT TAGAGAAACT TGCTGCAGTA 
CTGAAAGATA AACACTGTAA ACTCAACACA CTGATTCTGA ATAACAGCAC TATCTCAGAG 
GAAGATTTTC ATGTTCTGAC TGAAGCTTTT AATTCAAACC CAACAAATCT GACAGAGCTG 
AACTTGAGTG GGACTAAACT CACAAACTCA GGAATGAAGA GCTTCACAAC TCTGTTTCAA 
AACCAACAGT GTAGATTGGA AAAGCTGAAG TTCAGCAGCA TGTGTATTAC AGCAGAAGGT 
TGTGCTGCTT TGACTTCAGC ATTTAACTCA AACCCTTCAC ACCTGAAAGA GCTCAATCTG 
AGTGGGAATA AACTAGGAGA CTCAGGAGTA AGAAAAATCT CCAGTCTACT GAGAAACTCG 
CAGTGCATGC TGAAGATACT CGGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TTACATCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACCATCAGG AGTGAAGGAG CTCACTGGCT TACTACTCGA TCCACACTGT 
ACACTGACGA CACTGAGGTT TTTGAGTCCT GCTGCTGATG AATTCTGTCA GTTTGTGTCT 
GGAATTGTGG GTAAATACCC ATTACTCCTG AAAGAACTGA ATCTGAGTGA TCGTGGACTA 
GGGGACACAC GAGTGAATCA ACTCTCTGCT CTACTGCAGG ATAAACACTT TAGACTCAAC 
ACACTCATGC TGAATAATAA CAGGATTACA GCAGAAGGTT GTGCTGCTTT AACTTCAGCA 
TTTAATTTAA ATCCTTCACA CCTGATAGAG CTCAATCTGA GTGGGAATAA ACTAGAAGAC 
TCAGGAGTAG AGAAGATCTG TCCTCTGCTG GAAAATATAC AATGCCGATT AGAGAAACTG 
AGTCTTTCAG ACTGCAGTAT CAGGGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGAGT AGAAGCTGTA AATTCAGGTT TCTGAAGAGT 
TCTGCTGCAC AGGAAGCATG CGAGTATCTT ACTAATGTTC TGGGTATAAG TCCATTATTA 
CTGACAGAAC TGGATCTGAG TGAAGTTAAA TTAGGAGATC TGGACTGGGA GAAACTCTCT 
GCTCTTCTGA TGGACTCTCA TAGCAAAGTG GAGAAAATCA AGCTAAATAA TTGTGAGCTG 
ACAGAGAAAA GCTGTTCAGT TCTAGCTGCT GTTCTCTCCT CCAAAACCAT CCTGAAAGAG 
CTGAACCTGA ACAACAGTCG TCTGCTGGAC TCAGGAGTCA GAAAGATCTG TGAGGGACTG 
AAGAATCCTG TGTGTGAGCT GAAGATACTC AATCTTTCAG ACTGCAGTAT TACAGAAGAA 
GGTTATGAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAGC TCACTGAGTT ACTGCAGGAT 
CCACACTGTG CACTGAAGAC ACTGAGATTT TTAAGAAGTC CTGCTGCTGA TGAAGGCTGT 
CAGTTAGTAA CTGGAGTTGT GGGTAAAAAC CCGTTACTCC TGAAGGAACT GAATCTGAGT 
GATCATGAGC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGCCAGATCA ACACACTCAA TCTGAGTTAT TGCAGTATTA CAGAGAAACA GTGTGAGATC 
CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGGACCT CAGTGGGAAT 
GAACTAGGAG ACTTTCAAAG CCTTGGCTCT CTACTGAAGA ACCAACAATT CAAGCTGGAG 
AAACTAATTC TGTGTGTTTG CAGTATTAAA GAGAAGCAGT GTGAGATCCT GACTTCAGCT 
CTGAAATCAA ACCCATCACA CCTGAGAGAA CTGAACCTCA GTGGGAATGA ACTCAAGCAC 
AGAAGAGTGA AGCAATTTTT TGATGTGCTG AAAAAATCAA CCTGTAAACT GGAGCGACTG 
AGGTTAAGAT ACTGTAGTAT ACAAGCCGAT GATTGTACTG ATCTGGCTTC AGCTCTGAAA 
TCAAACCCAT CCCACCTGAG AGAACTGGAC CTCAGTGGAA ACAATCTAGG AGACTCTGGA 
GTGAAAAAAC TCAGTGATTT ACTGATAAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG 
AGTTATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTGAT 
GGCCTTAACC ATCTGCTGAG CAGTGTGGAT TGCAAGCTAA ATAAACTACA TCTGTGTGAT 
TGCAGTATTA CAGAGAAACA GTTGCTCATC CCAATCACAG CTACGTGTAT GGGCCTATTA 
CACCTGAGAG AACTGGACCT CAGTAAGAAC CAAATAAAAA ACCAAGGAGT GTACATGCTG 
TGTGATGTGC TGAAAAACCC ACGCTGTAGA CTCAAGAGGT TAAGACTAAA TGACTGTGGC 
ATTACAGATG TTACACCCTT AACTCAGTCT TTGACCAAGG ACAAAGCATT GAAGTTCTTA 
AAAGAGCTTG AACTGAGTAA AAATCAAATG GGATTCTTCG AAAAGCAAAA ACTCAGTGAC 
CTGCTGCGAG ACTCAAACTG TAAACTGAGG CTTGAGGAAG AAGAAGGTTA TTTTAGAGCT 
GGTGTAAAAC GGGTTACTAC ATTTTTGATG CCTCCATGGT CAAAAGTTCA AAAACCTGTG 
GATCTCACAG GAGAGGAAAG TTCATCATCA GATGAGGACG CCTCTGAAGA TGAGACTGAT 
CAGGCAGAGG AGGCTGATAA GCTCTCAAAT GAGGCGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208754.1
CDS	81..8462
Translation	MNNNNNLKDSGVKLLCTGLKNIKCELEILSLSDCNISEEGYKALASALRSNPSHLIELNLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAAEEFCQFVTGIVGKNPLLLKELYLSDHELGDTRVNQLSALLQDKHCTVSTLILHNCGLTKESCSALASALSSDTSLKELDLCNNNLQDSGVKKLQSELQNTNWRLKKLRLSKCDLTEESCSALASVLTSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSKLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELIELLQDPLCTLKTLRFLSPAADEACQFVTGIVGKNLLLLKELNLSDRQLGDTRVNQLSALLQDKHCTLNTLMLNNNRITAEGCAALTSAFNLNPSNLMELTLSGNKLEDSGVEKICSLLKNTQCRLKKLSLSDCRITEEGYKSLTLSLKSNSHLIELDLRGNDPGQSGVKELTDLQRGDCCKLKTVRFLKSSAAQEACDYLTKAVGKSPLLLTKLDLSEVKLGDLDGEKLSALLKDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLQDPHCTLNTLRFLSPAAEEACQFVTGIVGKNPILLKELNLSDHELGDTRVNQLSALLQDKHCKLNTLILKNSFISEEDCRVLAEAFNSNPSNLRELNLNGTKLTNSGMKSFNTLFQNQQCRLEKLKFSGISITAEGCAALTSAFSSNPSHLIELNLSGNTLEDSGVTEISSLLANSQCMLKILSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTALIPECALKTLRFLGPAAEEACQFVTGIVGKNPLLLKELNLSDRKLGDTRVNQLSALLQDKHCKPNTLQLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLRDSGVEEICPLLKNTQCRLERLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDERYRLKTLRLLKTTAAQEACDYLTKVVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIKLNNCKQIEKGCSVLAAVLSSKTILKEMNLNNSRLLDSGVLEICEGLKNPVCELKILKLSKCDLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKHSKLKRLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSCFSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCTLNILTLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLGDSGVEKICPLLKNTQCRLEKLNLSDCSITGKGYETLISALKSNSHLIELDLRGNDPGKSGVQKLTDLLRAGSCKLKTIRTLKGSAAQEACDYLTKAVGKSPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVEKIRMNNCKKLTEKSCLVLAAVLSSKTILKEMNLNNSRLLDSGVKVICEGLKNSKLEILKLSNCSITEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQSSWIPWKKVKCSMRFLSPAADEFCQFVSGIVGKNPLLLKELNLSDHVLADTQVNQLSALLQDKHCKLNTLILSDCSISGEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACEHLYKVLGGNLLLLRELNLTKHDLGPSGLEKLAAVLKDKHCKLNTLILNNSTISEEDFHVLTEAFNSNPTNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALTSAFNSNPSHLKELNLSGNKLGDSGVRKISSLLRNSQCMLKILGLSDCSISEEGYKALASALRSNPLHLIELDLRGNDPGPSGVKELTGLLLDPHCTLTTLRFLSPAADEFCQFVSGIVGKYPLLLKELNLSDRGLGDTRVNQLSALLQDKHFRLNTLMLNNNRITAEGCAALTSAFNLNPSHLIELNLSGNKLEDSGVEKICPLLENIQCRLEKLSLSDCSIREEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQSRSCKFRFLKSSAAQEACEYLTNVLGISPLLLTELDLSEVKLGDLDWEKLSALLMDSHSKVEKIKLNNCELTEKSCSVLAAVLSSKTILKELNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSITEEGYEALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCALKTLRFLRSPAADEGCQLVTGVVGKNPLLLKELNLSDHELGDTRVNQLSALLQDKHCQINTLNLSYCSITEKQCEILTSALKSNPSHLRELDLSGNELGDFQSLGSLLKNQQFKLEKLILCVCSIKEKQCEILTSALKSNPSHLRELNLSGNELKHRRVKQFFDVLKKSTCKLERLRLRYCSIQADDCTDLASALKSNPSHLRELDLSGNNLGDSGVKKLSDLLINQQFKLEKLRLSYCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGADGLNHLLSSVDCKLNKLHLCDCSITEKQLLIPITATCMGLLHLRELDLSKNQIKNQGVYMLCDVLKNPRCRLKRLRLNDCGITDVTPLTQSLTKDKALKFLKELELSKNQMGFFEKQKLSDLLRDSNCKLRLEEEEGYFRAGVKRVTTFLMPPWSKVQKPVDLTGEESSSSDEDASEDETDQAEEADKLSNEAD

Target ORF information:

RefSeq Version	XM_017353265.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-11p18.6 (si:ch211-11p18.6), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353265.1

ORF Insert Sequence:

ATGAATAATA ATAATAATTT AAAGGATTCA GGAGTGAAGC TGCTTTGCAC TGGACTGAAG 
AACATAAAGT GTGAATTGGA AATACTGAGT CTTTCAGACT GCAATATCAG TGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGAGATCA AACCCTTCAC ACCTGATAGA GCTGAATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAGCTCA CTGAGTTACT GCAGGATCCA 
CACTGTACAC TGAAGACACT GAGGTTTTTG AGTCCTGCAG CAGAAGAATT CTGTCAATTT 
GTGACTGGAA TTGTGGGTAA AAACCCGTTA CTCCTGAAAG AACTGTATCT GAGTGATCAT 
GAACTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGTACA 
GTCAGCACAC TCATTCTGCA TAATTGTGGT CTAACAAAGG AAAGCTGTTC AGCTCTGGCT 
TCAGCCTTGA GTTCAGACAC CAGTCTAAAA GAGCTCGACC TGTGCAACAA TAATCTGCAG 
GATTCAGGAG TGAAGAAACT CCAGAGCGAA TTACAAAATA CAAACTGGAG ACTGAAGAAA 
CTCAGGCTCA GTAAGTGTGA TCTAACAGAG GAAAGCTGTT CAGCTCTTGC TTCAGTTCTC 
ACATCAGACT CCAGCAGTCT GAAGGATCTT GACCTGAGCA ATAATAATCT GCAGGATTCA 
GGAGTGAAGC TGCTCTCTGA TGGACTGAAG GACAGTAAAC TGGAGAAACT CAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTAAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTGATAGAGT TACTGCAGGA TCCACTCTGT ACACTGAAGA CACTGAGGTT TTTGAGTCCT 
GCTGCTGATG AAGCCTGTCA GTTTGTGACT GGAATTGTGG GTAAAAACCT GTTACTCCTG 
AAAGAACTGA ATCTGAGTGA TCGTCAACTA GGAGACACAC GAGTGAATCA ACTCTCTGCT 
CTACTGCAGG ATAAACACTG TACACTCAAC ACACTCATGC TGAATAATAA CAGGATTACA 
GCAGAAGGTT GTGCTGCTTT GACTTCAGCA TTTAATTTAA ATCCATCAAA CCTGATGGAG 
CTCACTCTGA GTGGAAATAA ACTAGAAGAC TCAGGAGTAG AAAAGATCTG TTCTCTGCTG 
AAAAATACAC AGTGCAGATT GAAGAAACTG AGTCTTTCAG ACTGCAGGAT CACTGAAGAA 
GGATATAAAA GTTTGACTTT GTCCCTGAAG TCAAATTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAGCTCA CTGATTTACA ACGGGGTGAT 
TGCTGTAAAT TAAAAACCGT CAGGTTTTTA AAGAGTTCTG CTGCACAGGA AGCGTGTGAC 
TATCTTACTA AAGCTGTGGG TAAAAGTCCA TTATTACTGA CAAAACTGGA TCTGAGTGAA 
GTTAAATTAG GAGATCTGGA TGGAGAGAAA CTCTCTGCTC TTCTGAAGGA CTCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT GAGCTGACAG AGAAAAGCTG TTCAGTTCTA 
GCTGCTGTTC TCTCCTCCAA AACCATCCTG AAAGAGATGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGGCTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAACC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CTTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGAAGCTCAC TGACTTACTG CAGGATCCAC ACTGTACCCT GAATACACTG 
AGGTTTTTGA GTCCTGCCGC AGAAGAAGCC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCGATAC TTCTGAAAGA ACTGAATCTG AGTGATCATG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT GATTCTGAAA 
AACAGCTTTA TCTCAGAGGA AGATTGTCGT GTTCTGGCTG AAGCTTTTAA TTCAAACCCA 
TCAAATCTGA GAGAGCTGAA TTTGAATGGG ACTAAACTCA CAAACTCAGG AATGAAGAGC 
TTCAACACTC TGTTTCAAAA CCAACAGTGC AGACTGGAAA AGCTGAAGTT TAGCGGCATC 
AGTATCACAG CAGAAGGTTG TGCTGCTTTG ACTTCAGCAT TTAGCTCAAA CCCGTCACAC 
CTGATAGAGC TAAATCTGAG TGGGAATACA CTAGAAGACT CAGGAGTAAC AGAAATCTCC 
AGTCTACTGG CAAACTCACA GTGCATGCTG AAGATACTCA GTCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGCTTTG 
ATTCCAGAGT GTGCTCTGAA AACATTGAGG TTTTTGGGTC CTGCAGCAGA AGAAGCCTGT 
CAGTTTGTGA CTGGAATTGT GGGTAAAAAC CCGTTACTCC TGAAAGAACT GAATCTGAGT 
GATCGTAAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGTAAACCCA ACACACTTCA GCTGAATAAT AACAGGATTA CAGCAGAAGG TTGTGCTGCT 
TTAACTTCAG CATTTAATTT AAACCCTTCA CACCTAATAG AGCTCAATCT GAGTGGGAAT 
AAACTAAGAG ACTCAGGAGT AGAGGAGATC TGTCCTCTGC TGAAAAATAC ACAGTGCAGA 
CTGGAGAGAC TAAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTAAAGGA GCTCACTGAC TTACTGCAGG ATGAGCGATA TAGATTGAAG 
ACCCTCAGGC TTTTGAAAAC TACTGCAGCA CAAGAAGCGT GTGACTATCT TACTAAAGTT 
GTGGGTAAAA GTCCATTATT ACTGACAGAA CTGGATCTGA GTGAAGATAA ATTAGGAGAT 
CTGGATGGAG AGAAACTCTC TGCTCTTCTG ATGGACTCTC ATAGTAAAGT GGAGAAAATC 
AAGCTGAATA ATTGTAAGCA GATTGAAAAA GGCTGTTCAG TACTAGCTGC CGTTCTCTCC 
TCAAAAACAA TCCTAAAAGA GATGAACCTG AACAACAGTC GTCTGCTGGA CTCAGGAGTC 
TTAGAGATCT GTGAGGGACT GAAGAACCCT GTGTGTGAGC TGAAGATTCT CAAGCTCAGT 
AAGTGTGATT TAACAGAGGA AAGCTGTTCA GCTCTGGCTT CAGTTCTCAG ATCAGACTCC 
AGCAGTCTGA AGGATCTTGA CCTGAGCAAT AATAATCTGC AGGATTCAGG AGTGAAGTTG 
CTCTCTGATG GACTAAAGCA CAGTAAACTG AAGAGACTCA GTCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGACTTA 
CTGCAGGATC CACACTGTAC ACTAAAGACA CTGAGTTGTT TTAGTCCTGC TGCTGATGAA 
GGCTGTCAGT TTGTTACTGG AATTGTGGGT AAAAACCCGT TACTACTGAA AGAACTGAAT 
CTGAGTGATC GTGAGCTAGG AGACACACGG GTGAATCAAC TCTCTGCTCT ACTGCAAGAT 
AAACACTGTA CACTCAATAT ACTCACGCTG AATAATAACA GGATTACAGC AGAAGGTTGT 
GCTGCTTTGA CTTCAGCATT TAATTTAAAC CCTTCACACC TGATAGAGCT CAATCTGAGT 
GGAAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGAA AAATACCCAG 
TGCAGATTGG AGAAATTAAA TCTTTCAGAC TGCAGCATCA CTGGAAAAGG TTATGAAACA 
TTGATTTCAG CTTTGAAGTC AAACTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGGAAAT CAGGAGTGCA GAAGCTCACT GATTTGCTAC GAGCTGGAAG CTGTAAATTA 
AAAACCATCA GGACTTTGAA AGGTTCCGCT GCACAGGAAG CGTGTGACTA TCTAACTAAA 
GCTGTGGGTA AAAGTCCATT ATTACTGACA GAACTGGATC TGAGTGAAGA TAAATTAGGA 
GATCTGGATG GAGAGAAACT CTCTGCTCTT CTGATGGACT CTCATAGCAA AGTGGAGAAA 
ATCAGGATGA ATAACTGTAA AAAGCTGACA GAGAAAAGCT GTTTAGTTCT AGCTGCTGTT 
CTTTCATCCA AAACCATCTT GAAAGAGATG AACCTAAACA ACAGTCGTCT GCTGGACTCA 
GGAGTCAAAG TGATCTGTGA GGGACTGAAG AACTCTAAGC TGGAGATTTT AAAACTTTCA 
AATTGTAGTA TTACAGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCTGG AGTAAAGGAG 
CTCACTGAGT TACTGCAGAG TTCGTGGATT CCATGGAAAA AAGTGAAGTG TTCAATGAGG 
TTTTTGAGTC CTGCTGCTGA TGAATTTTGT CAGTTTGTGT CTGGAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCATGTAC TAGCAGACAC ACAAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGCAAACTCA ACACACTCAT TCTTTCAGAC 
TGCAGTATCA GTGGAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAAGAGCTC 
ACTGAGTTAC TGCAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTGATGAT 
GCAGATGAAG CCTGTGAACA TTTGTATAAA GTTCTGGGTG GAAACCTGTT ACTCCTGAGA 
GAGCTGAATC TGACTAAACA TGATCTTGGA CCATCAGGAT TAGAGAAACT TGCTGCAGTA 
CTGAAAGATA AACACTGTAA ACTCAACACA CTGATTCTGA ATAACAGCAC TATCTCAGAG 
GAAGATTTTC ATGTTCTGAC TGAAGCTTTT AATTCAAACC CAACAAATCT GACAGAGCTG 
AACTTGAGTG GGACTAAACT CACAAACTCA GGAATGAAGA GCTTCACAAC TCTGTTTCAA 
AACCAACAGT GTAGATTGGA AAAGCTGAAG TTCAGCAGCA TGTGTATTAC AGCAGAAGGT 
TGTGCTGCTT TGACTTCAGC ATTTAACTCA AACCCTTCAC ACCTGAAAGA GCTCAATCTG 
AGTGGGAATA AACTAGGAGA CTCAGGAGTA AGAAAAATCT CCAGTCTACT GAGAAACTCG 
CAGTGCATGC TGAAGATACT CGGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TTACATCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACCATCAGG AGTGAAGGAG CTCACTGGCT TACTACTCGA TCCACACTGT 
ACACTGACGA CACTGAGGTT TTTGAGTCCT GCTGCTGATG AATTCTGTCA GTTTGTGTCT 
GGAATTGTGG GTAAATACCC ATTACTCCTG AAAGAACTGA ATCTGAGTGA TCGTGGACTA 
GGGGACACAC GAGTGAATCA ACTCTCTGCT CTACTGCAGG ATAAACACTT TAGACTCAAC 
ACACTCATGC TGAATAATAA CAGGATTACA GCAGAAGGTT GTGCTGCTTT AACTTCAGCA 
TTTAATTTAA ATCCTTCACA CCTGATAGAG CTCAATCTGA GTGGGAATAA ACTAGAAGAC 
TCAGGAGTAG AGAAGATCTG TCCTCTGCTG GAAAATATAC AATGCCGATT AGAGAAACTG 
AGTCTTTCAG ACTGCAGTAT CAGGGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGAGT AGAAGCTGTA AATTCAGGTT TCTGAAGAGT 
TCTGCTGCAC AGGAAGCATG CGAGTATCTT ACTAATGTTC TGGGTATAAG TCCATTATTA 
CTGACAGAAC TGGATCTGAG TGAAGTTAAA TTAGGAGATC TGGACTGGGA GAAACTCTCT 
GCTCTTCTGA TGGACTCTCA TAGCAAAGTG GAGAAAATCA AGCTAAATAA TTGTGAGCTG 
ACAGAGAAAA GCTGTTCAGT TCTAGCTGCT GTTCTCTCCT CCAAAACCAT CCTGAAAGAG 
CTGAACCTGA ACAACAGTCG TCTGCTGGAC TCAGGAGTCA GAAAGATCTG TGAGGGACTG 
AAGAATCCTG TGTGTGAGCT GAAGATACTC AATCTTTCAG ACTGCAGTAT TACAGAAGAA 
GGTTATGAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAGC TCACTGAGTT ACTGCAGGAT 
CCACACTGTG CACTGAAGAC ACTGAGATTT TTAAGAAGTC CTGCTGCTGA TGAAGGCTGT 
CAGTTAGTAA CTGGAGTTGT GGGTAAAAAC CCGTTACTCC TGAAGGAACT GAATCTGAGT 
GATCATGAGC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGCCAGATCA ACACACTCAA TCTGAGTTAT TGCAGTATTA CAGAGAAACA GTGTGAGATC 
CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGGACCT CAGTGGGAAT 
GAACTAGGAG ACTTTCAAAG CCTTGGCTCT CTACTGAAGA ACCAACAATT CAAGCTGGAG 
AAACTAATTC TGTGTGTTTG CAGTATTAAA GAGAAGCAGT GTGAGATCCT GACTTCAGCT 
CTGAAATCAA ACCCATCACA CCTGAGAGAA CTGAACCTCA GTGGGAATGA ACTCAAGCAC 
AGAAGAGTGA AGCAATTTTT TGATGTGCTG AAAAAATCAA CCTGTAAACT GGAGCGACTG 
AGGTTAAGAT ACTGTAGTAT ACAAGCCGAT GATTGTACTG ATCTGGCTTC AGCTCTGAAA 
TCAAACCCAT CCCACCTGAG AGAACTGGAC CTCAGTGGAA ACAATCTAGG AGACTCTGGA 
GTGAAAAAAC TCAGTGATTT ACTGATAAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG 
AGTTATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTGAT 
GGCCTTAACC ATCTGCTGAG CAGTGTGGAT TGCAAGCTAA ATAAACTACA TCTGTGTGAT 
TGCAGTATTA CAGAGAAACA GTTGCTCATC CCAATCACAG CTACGTGTAT GGGCCTATTA 
CACCTGAGAG AACTGGACCT CAGTAAGAAC CAAATAAAAA ACCAAGGAGT GTACATGCTG 
TGTGATGTGC TGAAAAACCC ACGCTGTAGA CTCAAGAGGT TAAGACTAAA TGACTGTGGC 
ATTACAGATG TTACACCCTT AACTCAGTCT TTGACCAAGG ACAAAGCATT GAAGTTCTTA 
AAAGAGCTTG AACTGAGTAA AAATCAAATG GGATTCTTCG AAAAGCAAAA ACTCAGTGAC 
CTGCTGCGAG ACTCAAACTG TAAACTGAGG CTTGAGGAAG AAGAAGGTTA TTTTAGAGCT 
GGTGTAAAAC GGGTTACTAC ATTTTTGATG CCTCCATGGT CAAAAGTTCA AAAACCTGTG 
GATCTCACAG GAGAGGAAAG TTCATCATCA GATGAGGACG CCTCTGAAGA TGAGACTGAT 
CAGGCAGAGG AGGCTGATAA GCTCTCAAAT GAGGCGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

SI:CH211-11P18.6 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

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