COL4A3 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL4A3
Entrez Gene ID	553354
Full Name	collagen, type IV, alpha 3
Synonyms	zTumstatin
General protein information	Preferred Names collagen, type IV, alpha 3 Names collagen alpha-5(IV) chain type IV collagen alpha 3 chain
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_688921.9	XP_694013.8	collagen alpha-5(IV) chain
XM_009291834.2	XP_009290109.2	collagen alpha-5(IV) chain isoform X2
XM_688921.8	XP_694013.8	collagen alpha-5(IV) chain isoform X1

Pathways from BioSystems
Gene Ontology

The following COL4A3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa55287	XM_009291834.2 Latest version!	Danio rerio collagen, type IV, alpha 3 (col4a3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa55286	XM_688921.9 Latest version!	Danio rerio collagen, type IV, alpha 3 (col4a3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
ODa55286	XM_688921.8	Danio rerio collagen, type IV, alpha 3 (col4a3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL4A3 ( XM_009291834.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009290109.2 Danio rerio collagen, type IV, alpha 3 (col4a3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa55287
Clone ID Related Accession (Same CDS sequence)	XM_009291834.2
Accession Version	XM_009291834.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-5(IV) chain isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291834.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCATT GGGTATTATT GCGCGTGACA CTTGTCGGAA TGATGCTGCT GGGACTTCTG 
ACACCGACGC AAAGTAAAGG GGCTTGTGAT TGCAGTGGAA AATCAGGATG TGACTGCCAA 
GGAGTCAAAG GACAAAAGGG TGAGAAGGGT TCTCTTGGTT TGGTTGGTGA TGTTGGAGTT 
CAGGGGCATC AAGGAAAGGA AGGCCCTCCT GGATTTCCTG GTCTGAAGGG TTATTTGGGT 
ATACCAGGCA TCCCGGGACG TAAAGGTTCT AGGGGAATTT CAGGGACTCC TGGATTCTTT 
GGCCCTCCGG GACTACCGGG GCTTCCTGGG ATTCAGGGAC CAAGGGGGCC TCCTGGTCTC 
CCAGGATGCA ATGGAACAAA GGGTGAGCCT GGTCCTCCAG GTTTAATTGG GCCAATGGGG 
CGTCCTGGAA TCCAGGGAAG GCCAGGAATC CAAGGACCTA AGGGGGAGAC CATTTTTATT 
GCCGCTGCCG AACAAATGAT AGGACTTACG GGTCAACAAG GCAGAGCTGG AGATCCAGGT 
CCACCTGGGT TGCCAGGTGT TCAGGGAAAT ATTGGACCAG AGGGGCCTAA AGGTAACCCT 
GGACCACAAG GCATTCCTGG ATTTCCAGGA GAGAAGGGTA AAGAAGGTCG GAGCATGGGA 
ATACCTGGAC CCAGCGGTCA AAAGGGGGAG AGAGGACCCC CTGGTATACC TGGACAAAAA 
GGCATAAAGT TATTTGCATC AGGACCAAAG GAGCCTAAGG GTGACACTGG GGATTCTGGA 
GACAGAGGCA GCCCTGGAAT AATTGGTCTA CGAGGTCGTC CCGGTCCTAA GGGGGAGAAA 
GGGGAAAGAG GAGACCCTGG GGGTCCGGGT AAACCTGGAA AAGAGGGGCT ACAAGGAGAG 
TTAGGCTCTA AGGGAGGGAA GGGAGATAAA GGCTATCCCG GAGATACAGG CATAGAAGGG 
GAGAAGGGTC AGAAGGGTAA CCCTGGCCCC CCTGGTACGC CTGGAGAGTT CATTGTAATA 
AATACAGAGG TCAAAGGTCA AAAAGGGGAT CCTGGGCCAC CAGGACTTCT TGGTTTACGA 
GGTAGTGAAG GTTTCTATGG TTTTCCTGGA TATCAAGGAC TTCCAGGGAC CCCCATACAA 
GGACCTAATG GAAAACCAGG TCTACCAGGG TTTAAAGGTT TGAAAGGGGA TAAAGGAGAG 
CCTGGACCTG ACATCACAGG ACCCAAAGGT CGTGATGGCC AACGAGGGGA ACAAGGACCC 
CCTGGTGATC CTGGACTTCC GGGACCTTCA AATGTCATAA GCCGTTTGGG ACTCAAAGGA 
CTCAAGGGTG ATAAAGGTCT AATAGGCCCT GAGGGTCTGC GTGGGGATCC TGGGAACCAA 
GGAGAGCAAG GAGATCTGTG CAGGCTATGT GCTGCTTCTG CTGCTGATCC TGGTCCTCCA 
GGACAACCTG GGGATTCAGG CGAACCCGGC AAACCAGGTG CTGTTGGAAT TCAAGGTAAT 
AAAGGATCCA GAGGCTTGAA AGGTCCAATT GGTCCAGCTG GTCCTCCGGG TTTCTCAGGT 
CTTACAGGTT TTCCAGGGCC TTCAGGCCCC AAGGGAGAAA TGGGGATGAA ATATGAGATT 
GGGGAAAAAG GCTTTAAAGG AGACACTGGT GCAGTAGGAC TATTGGGATT AAGTGGACAA 
GATGGTTTAC CAGGCATCCC TGGCTTCAAA GGGCCACCGG GAGAAAAAGG AAAGCCAAGT 
GCTTTAGCTC CTAAAGGCGA TCAAGGATAT CCAGGTGTTG GTGGGGAACA AGGTAGCACA 
GGTCCAATGG GAAGTCAAGG GTCAATGGGT GTTGGGTCAC CAGGGGCACC TGGAGAAAAA 
GGAAATTTGG GAGTAGTGGG CCGGCAAGGA AATCTTGGAG ATCCAGGTGA AAAGGGATCT 
CCAGGACCAG TACAAACAAT ACCTGGAGAC CCAGGAGAGC CTGGACTGCC TGGTTCACCT 
GGTCTACCAG GACCTCCAGG TAATGAAGGT TGTGCTGGGG TTCAAGGATT CCCAGGATTG 
TGTGGGTTAA AAGGAGAGAA AGGACTCTCA ACACCAGGAG ATACTGGACT TACTGGTCCA 
AAAGGTGATA AGGGTGTGAA AGGGAGGCAA GGTTCACCTG GTTGTGGAAG TATAGGCCGT 
CCTGGACCTC CAGGGCCACC TGGAATGCCT GGCCCAAAGG GTGAAATAGC ATTTGGCTGC 
CCAGGGGCAA GGGGTAGAGA AGGTCTGCCA GGAGATGAAG GTTGCCTTGG TCCACCAGGA 
ATCTCTGGTC CTCCTGGATT ATCTGGATGT CGAGGTCAAA AAGGACAGGA TGGACCACCT 
GGAGAATTTG GTGTGCAGGG TTTTGATGGT TTTCCTGGAG TGGCTGGTGT TCCAGGATGC 
TGTATATCAG GGGCCAAAGG AGATGAAGGT CCCTCAGGAA TCAGAGGAGC AGATGGACCC 
CCTGGTTTGC CAGGTTTGAA AGGGCCACAA GGTGAAACTG GACTACCTGG ATTGAGCATA 
CCTGGTGTAA CAGGTGTGGT TGGGATACCT GGGATCTTTG GACCAGTTGG TGCAGTTGGT 
TTTCGGGGAC CAAAAGGTTT CCAGGGTCGA GATGGTTCCC CAGGATTTCC AGGTATACGG 
GGAGATCAGG GCAGTCCTGG AATGACTGGC CCACCTGGAG TAGATGGAAA TCCTGGACCA 
CCAGGGCTCC GGGGACCTAA AGGTTTAATG GGAAGGAATG GGGTCCCAGG TGATGAAGGA 
AAGCCTGGGG AGCCAGGAGA CCCTGGGCCC CGTGGCCCAA AGGGACAATC ATCAAATGTT 
ACGTCTCCAG GAGAACTTGG TCCTCCTGGC CTACCAGGTC TCCTTGGTCT GCCTGGTAGT 
AAAGGGGAAA TAGGACTTCC AGGCATTTGT GGGCAATGTG GGAAGAATGG CGTTCCAGGC 
AGTTCTGGTC CACCAGGCCC TCCTGGTGAC CCTGGCATAA AGGGGCTTCC TGGAAATCAG 
GGTCTTAATG GAAACTTTGG AGATCAAGGA GTTATGGGGT TTCCAGGAGT GAAAGGTGTT 
AAAGGTTCAC CTGGTTTTCA CGGGGAGCCT GGGATTCCTG GTGTTCCAGG ACCAAATGGA 
CCAAAGGGGA TCAAAGGTGA TCCTAGCCCC CCTGGGCCAG AGACTCCAGG ACTACTTGGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290109.2
CDS	22..3204
Translation	MDHWVLLRVTLVGMMLLGLLTPTQSKGACDCSGKSGCDCQGVKGQKGEKGSLGLVGDVGVQGHQGKEGPPGFPGLKGYLGIPGIPGRKGSRGISGTPGFFGPPGLPGLPGIQGPRGPPGLPGCNGTKGEPGPPGLIGPMGRPGIQGRPGIQGPKGETIFIAAAEQMIGLTGQQGRAGDPGPPGLPGVQGNIGPEGPKGNPGPQGIPGFPGEKGKEGRSMGIPGPSGQKGERGPPGIPGQKGIKLFASGPKEPKGDTGDSGDRGSPGIIGLRGRPGPKGEKGERGDPGGPGKPGKEGLQGELGSKGGKGDKGYPGDTGIEGEKGQKGNPGPPGTPGEFIVINTEVKGQKGDPGPPGLLGLRGSEGFYGFPGYQGLPGTPIQGPNGKPGLPGFKGLKGDKGEPGPDITGPKGRDGQRGEQGPPGDPGLPGPSNVISRLGLKGLKGDKGLIGPEGLRGDPGNQGEQGDLCRLCAASAADPGPPGQPGDSGEPGKPGAVGIQGNKGSRGLKGPIGPAGPPGFSGLTGFPGPSGPKGEMGMKYEIGEKGFKGDTGAVGLLGLSGQDGLPGIPGFKGPPGEKGKPSALAPKGDQGYPGVGGEQGSTGPMGSQGSMGVGSPGAPGEKGNLGVVGRQGNLGDPGEKGSPGPVQTIPGDPGEPGLPGSPGLPGPPGNEGCAGVQGFPGLCGLKGEKGLSTPGDTGLTGPKGDKGVKGRQGSPGCGSIGRPGPPGPPGMPGPKGEIAFGCPGARGREGLPGDEGCLGPPGISGPPGLSGCRGQKGQDGPPGEFGVQGFDGFPGVAGVPGCCISGAKGDEGPSGIRGADGPPGLPGLKGPQGETGLPGLSIPGVTGVVGIPGIFGPVGAVGFRGPKGFQGRDGSPGFPGIRGDQGSPGMTGPPGVDGNPGPPGLRGPKGLMGRNGVPGDEGKPGEPGDPGPRGPKGQSSNVTSPGELGPPGLPGLLGLPGSKGEIGLPGICGQCGKNGVPGSSGPPGPPGDPGIKGLPGNQGLNGNFGDQGVMGFPGVKGVKGSPGFHGEPGIPGVPGPNGPKGIKGDPSPPGPETPGLLG

Target ORF information:

RefSeq Version	XM_009291834.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type IV, alpha 3 (col4a3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291834.2

ORF Insert Sequence:

ATGGATCATT GGGTATTATT GCGCGTGACA CTTGTCGGAA TGATGCTGCT GGGACTTCTG 
ACACCGACGC AAAGTAAAGG GGCTTGTGAT TGCAGTGGAA AATCAGGATG TGACTGCCAA 
GGAGTCAAAG GACAAAAGGG TGAGAAGGGT TCTCTTGGTT TGGTTGGTGA TGTTGGAGTT 
CAGGGGCATC AAGGAAAGGA AGGCCCTCCT GGATTTCCTG GTCTGAAGGG TTATTTGGGT 
ATACCAGGCA TCCCGGGACG TAAAGGTTCT AGGGGAATTT CAGGGACTCC TGGATTCTTT 
GGCCCTCCGG GACTACCGGG GCTTCCTGGG ATTCAGGGAC CAAGGGGGCC TCCTGGTCTC 
CCAGGATGCA ATGGAACAAA GGGTGAGCCT GGTCCTCCAG GTTTAATTGG GCCAATGGGG 
CGTCCTGGAA TCCAGGGAAG GCCAGGAATC CAAGGACCTA AGGGGGAGAC CATTTTTATT 
GCCGCTGCCG AACAAATGAT AGGACTTACG GGTCAACAAG GCAGAGCTGG AGATCCAGGT 
CCACCTGGGT TGCCAGGTGT TCAGGGAAAT ATTGGACCAG AGGGGCCTAA AGGTAACCCT 
GGACCACAAG GCATTCCTGG ATTTCCAGGA GAGAAGGGTA AAGAAGGTCG GAGCATGGGA 
ATACCTGGAC CCAGCGGTCA AAAGGGGGAG AGAGGACCCC CTGGTATACC TGGACAAAAA 
GGCATAAAGT TATTTGCATC AGGACCAAAG GAGCCTAAGG GTGACACTGG GGATTCTGGA 
GACAGAGGCA GCCCTGGAAT AATTGGTCTA CGAGGTCGTC CCGGTCCTAA GGGGGAGAAA 
GGGGAAAGAG GAGACCCTGG GGGTCCGGGT AAACCTGGAA AAGAGGGGCT ACAAGGAGAG 
TTAGGCTCTA AGGGAGGGAA GGGAGATAAA GGCTATCCCG GAGATACAGG CATAGAAGGG 
GAGAAGGGTC AGAAGGGTAA CCCTGGCCCC CCTGGTACGC CTGGAGAGTT CATTGTAATA 
AATACAGAGG TCAAAGGTCA AAAAGGGGAT CCTGGGCCAC CAGGACTTCT TGGTTTACGA 
GGTAGTGAAG GTTTCTATGG TTTTCCTGGA TATCAAGGAC TTCCAGGGAC CCCCATACAA 
GGACCTAATG GAAAACCAGG TCTACCAGGG TTTAAAGGTT TGAAAGGGGA TAAAGGAGAG 
CCTGGACCTG ACATCACAGG ACCCAAAGGT CGTGATGGCC AACGAGGGGA ACAAGGACCC 
CCTGGTGATC CTGGACTTCC GGGACCTTCA AATGTCATAA GCCGTTTGGG ACTCAAAGGA 
CTCAAGGGTG ATAAAGGTCT AATAGGCCCT GAGGGTCTGC GTGGGGATCC TGGGAACCAA 
GGAGAGCAAG GAGATCTGTG CAGGCTATGT GCTGCTTCTG CTGCTGATCC TGGTCCTCCA 
GGACAACCTG GGGATTCAGG CGAACCCGGC AAACCAGGTG CTGTTGGAAT TCAAGGTAAT 
AAAGGATCCA GAGGCTTGAA AGGTCCAATT GGTCCAGCTG GTCCTCCGGG TTTCTCAGGT 
CTTACAGGTT TTCCAGGGCC TTCAGGCCCC AAGGGAGAAA TGGGGATGAA ATATGAGATT 
GGGGAAAAAG GCTTTAAAGG AGACACTGGT GCAGTAGGAC TATTGGGATT AAGTGGACAA 
GATGGTTTAC CAGGCATCCC TGGCTTCAAA GGGCCACCGG GAGAAAAAGG AAAGCCAAGT 
GCTTTAGCTC CTAAAGGCGA TCAAGGATAT CCAGGTGTTG GTGGGGAACA AGGTAGCACA 
GGTCCAATGG GAAGTCAAGG GTCAATGGGT GTTGGGTCAC CAGGGGCACC TGGAGAAAAA 
GGAAATTTGG GAGTAGTGGG CCGGCAAGGA AATCTTGGAG ATCCAGGTGA AAAGGGATCT 
CCAGGACCAG TACAAACAAT ACCTGGAGAC CCAGGAGAGC CTGGACTGCC TGGTTCACCT 
GGTCTACCAG GACCTCCAGG TAATGAAGGT TGTGCTGGGG TTCAAGGATT CCCAGGATTG 
TGTGGGTTAA AAGGAGAGAA AGGACTCTCA ACACCAGGAG ATACTGGACT TACTGGTCCA 
AAAGGTGATA AGGGTGTGAA AGGGAGGCAA GGTTCACCTG GTTGTGGAAG TATAGGCCGT 
CCTGGACCTC CAGGGCCACC TGGAATGCCT GGCCCAAAGG GTGAAATAGC ATTTGGCTGC 
CCAGGGGCAA GGGGTAGAGA AGGTCTGCCA GGAGATGAAG GTTGCCTTGG TCCACCAGGA 
ATCTCTGGTC CTCCTGGATT ATCTGGATGT CGAGGTCAAA AAGGACAGGA TGGACCACCT 
GGAGAATTTG GTGTGCAGGG TTTTGATGGT TTTCCTGGAG TGGCTGGTGT TCCAGGATGC 
TGTATATCAG GGGCCAAAGG AGATGAAGGT CCCTCAGGAA TCAGAGGAGC AGATGGACCC 
CCTGGTTTGC CAGGTTTGAA AGGGCCACAA GGTGAAACTG GACTACCTGG ATTGAGCATA 
CCTGGTGTAA CAGGTGTGGT TGGGATACCT GGGATCTTTG GACCAGTTGG TGCAGTTGGT 
TTTCGGGGAC CAAAAGGTTT CCAGGGTCGA GATGGTTCCC CAGGATTTCC AGGTATACGG 
GGAGATCAGG GCAGTCCTGG AATGACTGGC CCACCTGGAG TAGATGGAAA TCCTGGACCA 
CCAGGGCTCC GGGGACCTAA AGGTTTAATG GGAAGGAATG GGGTCCCAGG TGATGAAGGA 
AAGCCTGGGG AGCCAGGAGA CCCTGGGCCC CGTGGCCCAA AGGGACAATC ATCAAATGTT 
ACGTCTCCAG GAGAACTTGG TCCTCCTGGC CTACCAGGTC TCCTTGGTCT GCCTGGTAGT 
AAAGGGGAAA TAGGACTTCC AGGCATTTGT GGGCAATGTG GGAAGAATGG CGTTCCAGGC 
AGTTCTGGTC CACCAGGCCC TCCTGGTGAC CCTGGCATAA AGGGGCTTCC TGGAAATCAG 
GGTCTTAATG GAAACTTTGG AGATCAAGGA GTTATGGGGT TTCCAGGAGT GAAAGGTGTT 
AAAGGTTCAC CTGGTTTTCA CGGGGAGCCT GGGATTCCTG GTGTTCCAGG ACCAAATGGA 
CCAAAGGGGA TCAAAGGTGA TCCTAGCCCC CCTGGGCCAG AGACTCCAGG ACTACTTGGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A3 ( XM_688921.9 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_694013.8 Danio rerio collagen, type IV, alpha 3 (col4a3), mRNA.

Price & Availability↑

CloneID	ODa55286
Clone ID Related Accession (Same CDS sequence)	XM_688921.9 , XM_688921.8
Accession Version	XM_688921.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	collagen alpha-5(IV) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_688921.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCATT GGGTATTATT GCGCGTGACA CTTGTCGGAA TGATGCTGCT GGGACTTCTG 
ACACCGACGC AAAGTAAAGG GGCTTGTGAT TGCAGTGGAA AATCAGGATG TGACTGCCAA 
GGAGTCAAAG GACAAAAGGG TGAGAAGGGT TCTCTTGGTT TGGTTGGTGA TGTTGGAGTT 
CAGGGGCATC AAGGAAAGGA AGGCCCTCCT GGATTTCCTG GTCTGAAGGG TTATTTGGGT 
ATACCAGGCA TCCCGGGACG TAAAGGTTCT AGGGGAATTT CAGGGACTCC TGGATTCTTT 
GGCCCTCCGG GACTACCGGG GCTTCCTGGG ATTCAGGGAC CAAGGGGGCC TCCTGGTCTC 
CCAGGATGCA ATGGAACAAA GGGTGAGCCT GGTCCTCCAG GTTTAATTGG GCCAATGGGG 
CGTCCTGGAA TCCAGGGAAG GCCAGGAATC CAAGGACCTA AGGGGGAGAC CATTTTTATT 
GCCGCTGCCG AACAAATGAT AGGACTTACG GGTCAACAAG GCAGAGCTGG AGATCCAGGT 
CCACCTGGGT TGCCAGGTGT TCAGGGAAAT ATTGGACCAG AGGGGCCTAA AGGTAACCCT 
GGACCACAAG GCATTCCTGG ATTTCCAGGA GAGAAGGGTA AAGAAGGTCG GAGCATGGGA 
ATACCTGGAC CCAGCGGTCA AAAGGGGGAG AGAGGACCCC CTGGTATACC TGGACAAAAA 
GGCATAAAGT TATTTGCATC AGGACCAAAG GAGCCTAAGG GTGACACTGG GGATTCTGGA 
GACAGAGGCA GCCCTGGAAT AATTGGTCTA CGAGGTCGTC CCGGTCCTAA GGGGGAGAAA 
GGGGAAAGAG GAGACCCTGG GGGTCCGGGT AAACCTGGAA AAGAGGGGCT ACAAGGAGAG 
TTAGGCTCTA AGGGAGGGAA GGGAGATAAA GGCTATCCCG GAGATACAGG CATAGAAGGG 
GAGAAGGGTC AGAAGGGTAA CCCTGGCCCC CCTGGTACGC CTGGAGAGTT CATTGTAATA 
AATACAGAGG TCAAAGGTCA AAAAGGGGAT CCTGGGCCAC CAGGACTTCT TGGTTTACGA 
GGTAGTGAAG GTTTCTATGG TTTTCCTGGA TATCAAGGAC TTCCAGGGAC CCCCATACAA 
GGACCTAATG GAAAACCAGG TCTACCAGGG TTTAAAGGTT TGAAAGGGGA TAAAGGAGAG 
CCTGGACCTG ACATCACAGG ACCCAAAGGT CGTGATGGCC AACGAGGGGA ACAAGGACCC 
CCTGGTGATC CTGGACTTCC GGGACCTTCA AATGTCATAA GCCGTTTGGG ACTCAAAGGA 
CTCAAGGGTG ATAAAGGTCT AATAGGCCCT GAGGGTCTGC GTGGGGATCC TGGGAACCAA 
GGAGAGCAAG GAGATCTGTG CAGGCTATGT GCTGCTTCTG CTGCTGATCC TGGTCCTCCA 
GGACAACCTG GGGATTCAGG CGAACCCGGC AAACCAGGTG CTGTTGGAAT TCAAGGTAAT 
AAAGGATCCA GAGGCTTGAA AGGTCCAATT GGTCCAGCTG GTCCTCCGGG TTTCTCAGGT 
CTTACAGGTT TTCCAGGGCC TTCAGGCCCC AAGGGAGAAA TGGGGATGAA ATATGAGATT 
GGGGAAAAAG GCTTTAAAGG AGACACTGGT GCAGTAGGAC TATTGGGATT AAGTGGACAA 
GATGGTTTAC CAGGCATCCC TGGCTTCAAA GGGCCACCGG GAGAAAAAGG AAAGCCAAGT 
GCTTTAGCTC CTAAAGGCGA TCAAGGATAT CCAGGTGTTG GTGGGGAACA AGGTAGCACA 
GGTCCAATGG GAAGTCAAGG GTCAATGGGT GTTGGGTCAC CAGGGGCACC TGGAGAAAAA 
GGAAATTTGG GAGTAGTGGG CCGGCAAGGA AATCTTGGAG ATCCAGGTGA AAAGGGATCT 
CCAGGACCAG TACAAACAAT ACCTGGAGAC CCAGGAGAGC CTGGACTGCC TGGTTCACCT 
GGTCTACCAG GACCTCCAGG TAATGAAGGT TGTGCTGGGG TTCAAGGATT CCCAGGATTG 
TGTGGGTTAA AAGGAGAGAA AGGACTCTCA ACACCAGGAG ATACTGGACT TACTGGTCCA 
AAAGGTGATA AGGGTGTGAA AGGGAGGCAA GGTTCACCTG GTTGTGGAAG TATAGGCCGT 
CCTGGACCTC CAGGGCCACC TGGAATGCCT GGCCCAAAGG GTGAAATAGC ATTTGGCTGC 
CCAGGGGCAA GGGGTAGAGA AGGTCTGCCA GGAGATGAAG GTTGCCTTGG TCCACCAGGA 
ATCTCTGGTC CTCCTGGATT ATCTGGATGT CGAGGTCAAA AAGGACAGGA TGGACCACCT 
GGAGAATTTG GTGTGCAGGG TTTTGATGGT TTTCCTGGAG TGGCTGGTGT TCCAGGATGC 
TGTATATCAG GGGCCAAAGG AGATGAAGGT CCCTCAGGAA TCAGAGGAGC AGATGGACCC 
CCTGGTTTGC CAGGTTTGAA AGGGCCACAA GGTGAAACTG GACTACCTGG ATTGAGCATA 
CCTGGTGTAA CAGGTGTGGT TGGGATACCT GGGATCTTTG GACCAGTTGG TGCAGTTGGT 
TTTCGGGGAC CAAAAGGTTT CCAGGGTCGA GATGGTTCCC CAGGATTTCC AGGTATACGG 
GGAGATCAGG GCAGTCCTGG AATGACTGGC CCACCTGGAG TAGATGGAAA TCCTGGACCA 
CCAGGGCTCC GGGGACCTAA AGGTTTAATG GGAAGGAATG GGGTCCCAGG TGATGAAGGA 
AAGCCTGGGG AGCCAGGAGA CCCTGGGCCC CGTGGCCCAA AGGGACAATC ATCAAATGTT 
ACGTCTCCAG GAGAACTTGG TCCTCCTGGC CTACCAGGTC TCCTTGGTCT GCCTGGTAGT 
AAAGGGGAAA TAGGACTTCC AGGCATTTGT GGGCAATGTG GGAAGAATGG CGTTCCAGGC 
AGTTCTGGTC CACCAGGCCC TCCTGGTGAC CCTGGCATAA AGGGGCTTCC TGGAAATCAG 
GGTCTTAATG GAAACTTTGG AGATCAAGGA GTTATGGGGT TTCCAGGAGT GAAAGGTGTT 
AAAGGTTCAC CTGGTTTTCA CGGGGAGCCT GGGATTCCTG GTGTTCCAGG ACCAAATGGA 
CCAAAGGGGA TCAAAGGTGA TCCTAGCCCC CCTGGGCCAG AGACTCCAGG ACTACTTGGT 
GAGAAGGGTG AACCAGGGTG TGCTGGGGGT ACAGGGAAGA AAGGTGACAA AGGTATATGT 
GGAATAAATG GGATCCCAGG TTTCTCTGGA GTACCTGGGG AAAGAGGAGT AACTGGAGTG 
ACAGGAGATC CAGGACCCCC AGGAGAAGCT GGTGATGATG GGTTTGATGG ATTTCCAGGT 
CCACCTGGAT GTGAAGGACC CTGTGGTCCA CCAGGTGTTC CTGGCTCGCC TGGAGATCCT 
GGACCTCCAG GACCTCGGGG AGAACAAGGC AGAGATGGCA TTCCAGGGTC AGCCGGAGAA 
AAAGGAGACA GAGGGTGCAC TATGACTGGG GATTCTGGCC TACCTGGCAT CAAGGGTGAG 
AAAGGCATTA AAGGCACCCG TGGACCACCA GGGCCATGTG GGCCACCAGG ACCCTGTGGC 
CCATCTGGAG AAATTGGTTC TCCAGGCAAA CCCGGAGAAA AAGGGCAACG TGGGTGTTCT 
GGACCACCTG GTGTCTGTAT TAGTGGCCCA AAAGGAGAGC GAGGACCTAA TGGGTTTCCT 
GGTCCACCAG GCCTCCAAGG ACCATGTGGG CCTCCTGGAG ACCCAGGTCC AGGCCTTAAG 
GGAGATAAAG GCTGTGTCGG GCCCCAAGGA CGCGTAGGAC CACCTGGTTG TACTGGTGAC 
CGGGGAACTC CAGGTGAACC AGGTAAAGAT GGAGATTGTG GTTTAAGGGG TCCAAAAGGA 
AATATAGGGC CTTTGGGTAG AGTTGGACCA GATGGACAAA AAGGACAGTG TGGTCCTGTT 
GGACCTGCTG GATTAAAAGG TTGTAAAGGA TCTCCTGGAA CTAAAGGGCC AAAAGGCTGT 
GATTACACTG TTGAGATCTT TTCTACACCA GGAGATCGCG GATTTCGTGG AAAAACCGGT 
CAGAAAGGAG AACCAGGGGA TAATGGAGAT AAAGGATTGC GAGGTCTAAA AGGAACAAAA 
GGTCAAAAAG GAGATATAGG AGCAAATGGA CCTGATGGAT CTAAGGGGAT GCTCGGGCTG 
CAGGGAGACA GTGGAGAAAA AGGACTAAAA GGTGCTCGGG GTTTGACAGG CGAAGTGGGT 
GCCAAGGGTG CACCAGGTGC AAAAGGTCCA CAACTGAGAA AAAGAGACGG ATTCCTTTTC 
ACCAGACATA GTCAGACAAC GGTTATACCA GAATGTCCTG CGGGGTCGAA ACGTTTATAC 
ACTGGATATT CACTTCTCTT CATCAACGGC AATAACCGAG GACATGGACA AGACCTAGGA 
ACTCTGGGAA GTTGTTTGCC GATGTTCAAC ACCATGCCTT TTATGGTGTG TAACCGTGAT 
GAAACTTGTC GCTACGCCTC TCGAAATGAC TACTCCTATT GGCTGTCAAC AGACACACCA 
ATGTTACCTG ACCAGCAAAT GATGTCTGGT GAAATACTGA AATGGTACAT CAGCAGGTGT 
TCCGTATGCG AGGCAATAGC TAATGTTATA GCAATTCACA GTCAGACCAT TAACATCCCA 
CAATGCCCTG TTGGGTGGCT TTCACTATGG GAAGGTTACT CCTTTGTAAT GCAAACAGGA 
GTTGGCGCAG AAGGTTCTGG GCAACCATTG GTCTCTCCTG GATCCTGTCT GGAACAATTC 
CGGAAGATTC CATTCATAGA GTGTCATGGT CGCGGCACCT GCAACTTCTA TCCTGATTCC 
TACAGCTACT GGCTTGCTTC ACTCGACCAC ACCAATATGT TCAGTATGCC AAACCGCCAA 
ACAGCAAAAC AGAAAGAAAT CATCAGCCGC TGTCAGGTCT GTATGAAGCA ACAGCCTCAA 
GGTGACCTTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_694013.8
CDS	57..5051
Translation	MDHWVLLRVTLVGMMLLGLLTPTQSKGACDCSGKSGCDCQGVKGQKGEKGSLGLVGDVGVQGHQGKEGPPGFPGLKGYLGIPGIPGRKGSRGISGTPGFFGPPGLPGLPGIQGPRGPPGLPGCNGTKGEPGPPGLIGPMGRPGIQGRPGIQGPKGETIFIAAAEQMIGLTGQQGRAGDPGPPGLPGVQGNIGPEGPKGNPGPQGIPGFPGEKGKEGRSMGIPGPSGQKGERGPPGIPGQKGIKLFASGPKEPKGDTGDSGDRGSPGIIGLRGRPGPKGEKGERGDPGGPGKPGKEGLQGELGSKGGKGDKGYPGDTGIEGEKGQKGNPGPPGTPGEFIVINTEVKGQKGDPGPPGLLGLRGSEGFYGFPGYQGLPGTPIQGPNGKPGLPGFKGLKGDKGEPGPDITGPKGRDGQRGEQGPPGDPGLPGPSNVISRLGLKGLKGDKGLIGPEGLRGDPGNQGEQGDLCRLCAASAADPGPPGQPGDSGEPGKPGAVGIQGNKGSRGLKGPIGPAGPPGFSGLTGFPGPSGPKGEMGMKYEIGEKGFKGDTGAVGLLGLSGQDGLPGIPGFKGPPGEKGKPSALAPKGDQGYPGVGGEQGSTGPMGSQGSMGVGSPGAPGEKGNLGVVGRQGNLGDPGEKGSPGPVQTIPGDPGEPGLPGSPGLPGPPGNEGCAGVQGFPGLCGLKGEKGLSTPGDTGLTGPKGDKGVKGRQGSPGCGSIGRPGPPGPPGMPGPKGEIAFGCPGARGREGLPGDEGCLGPPGISGPPGLSGCRGQKGQDGPPGEFGVQGFDGFPGVAGVPGCCISGAKGDEGPSGIRGADGPPGLPGLKGPQGETGLPGLSIPGVTGVVGIPGIFGPVGAVGFRGPKGFQGRDGSPGFPGIRGDQGSPGMTGPPGVDGNPGPPGLRGPKGLMGRNGVPGDEGKPGEPGDPGPRGPKGQSSNVTSPGELGPPGLPGLLGLPGSKGEIGLPGICGQCGKNGVPGSSGPPGPPGDPGIKGLPGNQGLNGNFGDQGVMGFPGVKGVKGSPGFHGEPGIPGVPGPNGPKGIKGDPSPPGPETPGLLGEKGEPGCAGGTGKKGDKGICGINGIPGFSGVPGERGVTGVTGDPGPPGEAGDDGFDGFPGPPGCEGPCGPPGVPGSPGDPGPPGPRGEQGRDGIPGSAGEKGDRGCTMTGDSGLPGIKGEKGIKGTRGPPGPCGPPGPCGPSGEIGSPGKPGEKGQRGCSGPPGVCISGPKGERGPNGFPGPPGLQGPCGPPGDPGPGLKGDKGCVGPQGRVGPPGCTGDRGTPGEPGKDGDCGLRGPKGNIGPLGRVGPDGQKGQCGPVGPAGLKGCKGSPGTKGPKGCDYTVEIFSTPGDRGFRGKTGQKGEPGDNGDKGLRGLKGTKGQKGDIGANGPDGSKGMLGLQGDSGEKGLKGARGLTGEVGAKGAPGAKGPQLRKRDGFLFTRHSQTTVIPECPAGSKRLYTGYSLLFINGNNRGHGQDLGTLGSCLPMFNTMPFMVCNRDETCRYASRNDYSYWLSTDTPMLPDQQMMSGEILKWYISRCSVCEAIANVIAIHSQTINIPQCPVGWLSLWEGYSFVMQTGVGAEGSGQPLVSPGSCLEQFRKIPFIECHGRGTCNFYPDSYSYWLASLDHTNMFSMPNRQTAKQKEIISRCQVCMKQQPQGDLA

Target ORF information:

RefSeq Version	XM_688921.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type IV, alpha 3 (col4a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_688921.9

ORF Insert Sequence:

ATGGATCATT GGGTATTATT GCGCGTGACA CTTGTCGGAA TGATGCTGCT GGGACTTCTG 
ACACCGACGC AAAGTAAAGG GGCTTGTGAT TGCAGTGGAA AATCAGGATG TGACTGCCAA 
GGAGTCAAAG GACAAAAGGG TGAGAAGGGT TCTCTTGGTT TGGTTGGTGA TGTTGGAGTT 
CAGGGGCATC AAGGAAAGGA AGGCCCTCCT GGATTTCCTG GTCTGAAGGG TTATTTGGGT 
ATACCAGGCA TCCCGGGACG TAAAGGTTCT AGGGGAATTT CAGGGACTCC TGGATTCTTT 
GGCCCTCCGG GACTACCGGG GCTTCCTGGG ATTCAGGGAC CAAGGGGGCC TCCTGGTCTC 
CCAGGATGCA ATGGAACAAA GGGTGAGCCT GGTCCTCCAG GTTTAATTGG GCCAATGGGG 
CGTCCTGGAA TCCAGGGAAG GCCAGGAATC CAAGGACCTA AGGGGGAGAC CATTTTTATT 
GCCGCTGCCG AACAAATGAT AGGACTTACG GGTCAACAAG GCAGAGCTGG AGATCCAGGT 
CCACCTGGGT TGCCAGGTGT TCAGGGAAAT ATTGGACCAG AGGGGCCTAA AGGTAACCCT 
GGACCACAAG GCATTCCTGG ATTTCCAGGA GAGAAGGGTA AAGAAGGTCG GAGCATGGGA 
ATACCTGGAC CCAGCGGTCA AAAGGGGGAG AGAGGACCCC CTGGTATACC TGGACAAAAA 
GGCATAAAGT TATTTGCATC AGGACCAAAG GAGCCTAAGG GTGACACTGG GGATTCTGGA 
GACAGAGGCA GCCCTGGAAT AATTGGTCTA CGAGGTCGTC CCGGTCCTAA GGGGGAGAAA 
GGGGAAAGAG GAGACCCTGG GGGTCCGGGT AAACCTGGAA AAGAGGGGCT ACAAGGAGAG 
TTAGGCTCTA AGGGAGGGAA GGGAGATAAA GGCTATCCCG GAGATACAGG CATAGAAGGG 
GAGAAGGGTC AGAAGGGTAA CCCTGGCCCC CCTGGTACGC CTGGAGAGTT CATTGTAATA 
AATACAGAGG TCAAAGGTCA AAAAGGGGAT CCTGGGCCAC CAGGACTTCT TGGTTTACGA 
GGTAGTGAAG GTTTCTATGG TTTTCCTGGA TATCAAGGAC TTCCAGGGAC CCCCATACAA 
GGACCTAATG GAAAACCAGG TCTACCAGGG TTTAAAGGTT TGAAAGGGGA TAAAGGAGAG 
CCTGGACCTG ACATCACAGG ACCCAAAGGT CGTGATGGCC AACGAGGGGA ACAAGGACCC 
CCTGGTGATC CTGGACTTCC GGGACCTTCA AATGTCATAA GCCGTTTGGG ACTCAAAGGA 
CTCAAGGGTG ATAAAGGTCT AATAGGCCCT GAGGGTCTGC GTGGGGATCC TGGGAACCAA 
GGAGAGCAAG GAGATCTGTG CAGGCTATGT GCTGCTTCTG CTGCTGATCC TGGTCCTCCA 
GGACAACCTG GGGATTCAGG CGAACCCGGC AAACCAGGTG CTGTTGGAAT TCAAGGTAAT 
AAAGGATCCA GAGGCTTGAA AGGTCCAATT GGTCCAGCTG GTCCTCCGGG TTTCTCAGGT 
CTTACAGGTT TTCCAGGGCC TTCAGGCCCC AAGGGAGAAA TGGGGATGAA ATATGAGATT 
GGGGAAAAAG GCTTTAAAGG AGACACTGGT GCAGTAGGAC TATTGGGATT AAGTGGACAA 
GATGGTTTAC CAGGCATCCC TGGCTTCAAA GGGCCACCGG GAGAAAAAGG AAAGCCAAGT 
GCTTTAGCTC CTAAAGGCGA TCAAGGATAT CCAGGTGTTG GTGGGGAACA AGGTAGCACA 
GGTCCAATGG GAAGTCAAGG GTCAATGGGT GTTGGGTCAC CAGGGGCACC TGGAGAAAAA 
GGAAATTTGG GAGTAGTGGG CCGGCAAGGA AATCTTGGAG ATCCAGGTGA AAAGGGATCT 
CCAGGACCAG TACAAACAAT ACCTGGAGAC CCAGGAGAGC CTGGACTGCC TGGTTCACCT 
GGTCTACCAG GACCTCCAGG TAATGAAGGT TGTGCTGGGG TTCAAGGATT CCCAGGATTG 
TGTGGGTTAA AAGGAGAGAA AGGACTCTCA ACACCAGGAG ATACTGGACT TACTGGTCCA 
AAAGGTGATA AGGGTGTGAA AGGGAGGCAA GGTTCACCTG GTTGTGGAAG TATAGGCCGT 
CCTGGACCTC CAGGGCCACC TGGAATGCCT GGCCCAAAGG GTGAAATAGC ATTTGGCTGC 
CCAGGGGCAA GGGGTAGAGA AGGTCTGCCA GGAGATGAAG GTTGCCTTGG TCCACCAGGA 
ATCTCTGGTC CTCCTGGATT ATCTGGATGT CGAGGTCAAA AAGGACAGGA TGGACCACCT 
GGAGAATTTG GTGTGCAGGG TTTTGATGGT TTTCCTGGAG TGGCTGGTGT TCCAGGATGC 
TGTATATCAG GGGCCAAAGG AGATGAAGGT CCCTCAGGAA TCAGAGGAGC AGATGGACCC 
CCTGGTTTGC CAGGTTTGAA AGGGCCACAA GGTGAAACTG GACTACCTGG ATTGAGCATA 
CCTGGTGTAA CAGGTGTGGT TGGGATACCT GGGATCTTTG GACCAGTTGG TGCAGTTGGT 
TTTCGGGGAC CAAAAGGTTT CCAGGGTCGA GATGGTTCCC CAGGATTTCC AGGTATACGG 
GGAGATCAGG GCAGTCCTGG AATGACTGGC CCACCTGGAG TAGATGGAAA TCCTGGACCA 
CCAGGGCTCC GGGGACCTAA AGGTTTAATG GGAAGGAATG GGGTCCCAGG TGATGAAGGA 
AAGCCTGGGG AGCCAGGAGA CCCTGGGCCC CGTGGCCCAA AGGGACAATC ATCAAATGTT 
ACGTCTCCAG GAGAACTTGG TCCTCCTGGC CTACCAGGTC TCCTTGGTCT GCCTGGTAGT 
AAAGGGGAAA TAGGACTTCC AGGCATTTGT GGGCAATGTG GGAAGAATGG CGTTCCAGGC 
AGTTCTGGTC CACCAGGCCC TCCTGGTGAC CCTGGCATAA AGGGGCTTCC TGGAAATCAG 
GGTCTTAATG GAAACTTTGG AGATCAAGGA GTTATGGGGT TTCCAGGAGT GAAAGGTGTT 
AAAGGTTCAC CTGGTTTTCA CGGGGAGCCT GGGATTCCTG GTGTTCCAGG ACCAAATGGA 
CCAAAGGGGA TCAAAGGTGA TCCTAGCCCC CCTGGGCCAG AGACTCCAGG ACTACTTGGT 
GAGAAGGGTG AACCAGGGTG TGCTGGGGGT ACAGGGAAGA AAGGTGACAA AGGTATATGT 
GGAATAAATG GGATCCCAGG TTTCTCTGGA GTACCTGGGG AAAGAGGAGT AACTGGAGTG 
ACAGGAGATC CAGGACCCCC AGGAGAAGCT GGTGATGATG GGTTTGATGG ATTTCCAGGT 
CCACCTGGAT GTGAAGGACC CTGTGGTCCA CCAGGTGTTC CTGGCTCGCC TGGAGATCCT 
GGACCTCCAG GACCTCGGGG AGAACAAGGC AGAGATGGCA TTCCAGGGTC AGCCGGAGAA 
AAAGGAGACA GAGGGTGCAC TATGACTGGG GATTCTGGCC TACCTGGCAT CAAGGGTGAG 
AAAGGCATTA AAGGCACCCG TGGACCACCA GGGCCATGTG GGCCACCAGG ACCCTGTGGC 
CCATCTGGAG AAATTGGTTC TCCAGGCAAA CCCGGAGAAA AAGGGCAACG TGGGTGTTCT 
GGACCACCTG GTGTCTGTAT TAGTGGCCCA AAAGGAGAGC GAGGACCTAA TGGGTTTCCT 
GGTCCACCAG GCCTCCAAGG ACCATGTGGG CCTCCTGGAG ACCCAGGTCC AGGCCTTAAG 
GGAGATAAAG GCTGTGTCGG GCCCCAAGGA CGCGTAGGAC CACCTGGTTG TACTGGTGAC 
CGGGGAACTC CAGGTGAACC AGGTAAAGAT GGAGATTGTG GTTTAAGGGG TCCAAAAGGA 
AATATAGGGC CTTTGGGTAG AGTTGGACCA GATGGACAAA AAGGACAGTG TGGTCCTGTT 
GGACCTGCTG GATTAAAAGG TTGTAAAGGA TCTCCTGGAA CTAAAGGGCC AAAAGGCTGT 
GATTACACTG TTGAGATCTT TTCTACACCA GGAGATCGCG GATTTCGTGG AAAAACCGGT 
CAGAAAGGAG AACCAGGGGA TAATGGAGAT AAAGGATTGC GAGGTCTAAA AGGAACAAAA 
GGTCAAAAAG GAGATATAGG AGCAAATGGA CCTGATGGAT CTAAGGGGAT GCTCGGGCTG 
CAGGGAGACA GTGGAGAAAA AGGACTAAAA GGTGCTCGGG GTTTGACAGG CGAAGTGGGT 
GCCAAGGGTG CACCAGGTGC AAAAGGTCCA CAACTGAGAA AAAGAGACGG ATTCCTTTTC 
ACCAGACATA GTCAGACAAC GGTTATACCA GAATGTCCTG CGGGGTCGAA ACGTTTATAC 
ACTGGATATT CACTTCTCTT CATCAACGGC AATAACCGAG GACATGGACA AGACCTAGGA 
ACTCTGGGAA GTTGTTTGCC GATGTTCAAC ACCATGCCTT TTATGGTGTG TAACCGTGAT 
GAAACTTGTC GCTACGCCTC TCGAAATGAC TACTCCTATT GGCTGTCAAC AGACACACCA 
ATGTTACCTG ACCAGCAAAT GATGTCTGGT GAAATACTGA AATGGTACAT CAGCAGGTGT 
TCCGTATGCG AGGCAATAGC TAATGTTATA GCAATTCACA GTCAGACCAT TAACATCCCA 
CAATGCCCTG TTGGGTGGCT TTCACTATGG GAAGGTTACT CCTTTGTAAT GCAAACAGGA 
GTTGGCGCAG AAGGTTCTGG GCAACCATTG GTCTCTCCTG GATCCTGTCT GGAACAATTC 
CGGAAGATTC CATTCATAGA GTGTCATGGT CGCGGCACCT GCAACTTCTA TCCTGATTCC 
TACAGCTACT GGCTTGCTTC ACTCGACCAC ACCAATATGT TCAGTATGCC AAACCGCCAA 
ACAGCAAAAC AGAAAGAAAT CATCAGCCGC TGTCAGGTCT GTATGAAGCA ACAGCCTCAA 
GGTGACCTTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A3 ( XM_688921.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_694013.8 Danio rerio collagen, type IV, alpha 3 (col4a3), transcript variant X1, mRNA.

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CloneID	ODa55286
Clone ID Related Accession (Same CDS sequence)	XM_688921.9 , XM_688921.8
Accession Version	XM_688921.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-5(IV) chain isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_688921.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCATT GGGTATTATT GCGCGTGACA CTTGTCGGAA TGATGCTGCT GGGACTTCTG 
ACACCGACGC AAAGTAAAGG GGCTTGTGAT TGCAGTGGAA AATCAGGATG TGACTGCCAA 
GGAGTCAAAG GACAAAAGGG TGAGAAGGGT TCTCTTGGTT TGGTTGGTGA TGTTGGAGTT 
CAGGGGCATC AAGGAAAGGA AGGCCCTCCT GGATTTCCTG GTCTGAAGGG TTATTTGGGT 
ATACCAGGCA TCCCGGGACG TAAAGGTTCT AGGGGAATTT CAGGGACTCC TGGATTCTTT 
GGCCCTCCGG GACTACCGGG GCTTCCTGGG ATTCAGGGAC CAAGGGGGCC TCCTGGTCTC 
CCAGGATGCA ATGGAACAAA GGGTGAGCCT GGTCCTCCAG GTTTAATTGG GCCAATGGGG 
CGTCCTGGAA TCCAGGGAAG GCCAGGAATC CAAGGACCTA AGGGGGAGAC CATTTTTATT 
GCCGCTGCCG AACAAATGAT AGGACTTACG GGTCAACAAG GCAGAGCTGG AGATCCAGGT 
CCACCTGGGT TGCCAGGTGT TCAGGGAAAT ATTGGACCAG AGGGGCCTAA AGGTAACCCT 
GGACCACAAG GCATTCCTGG ATTTCCAGGA GAGAAGGGTA AAGAAGGTCG GAGCATGGGA 
ATACCTGGAC CCAGCGGTCA AAAGGGGGAG AGAGGACCCC CTGGTATACC TGGACAAAAA 
GGCATAAAGT TATTTGCATC AGGACCAAAG GAGCCTAAGG GTGACACTGG GGATTCTGGA 
GACAGAGGCA GCCCTGGAAT AATTGGTCTA CGAGGTCGTC CCGGTCCTAA GGGGGAGAAA 
GGGGAAAGAG GAGACCCTGG GGGTCCGGGT AAACCTGGAA AAGAGGGGCT ACAAGGAGAG 
TTAGGCTCTA AGGGAGGGAA GGGAGATAAA GGCTATCCCG GAGATACAGG CATAGAAGGG 
GAGAAGGGTC AGAAGGGTAA CCCTGGCCCC CCTGGTACGC CTGGAGAGTT CATTGTAATA 
AATACAGAGG TCAAAGGTCA AAAAGGGGAT CCTGGGCCAC CAGGACTTCT TGGTTTACGA 
GGTAGTGAAG GTTTCTATGG TTTTCCTGGA TATCAAGGAC TTCCAGGGAC CCCCATACAA 
GGACCTAATG GAAAACCAGG TCTACCAGGG TTTAAAGGTT TGAAAGGGGA TAAAGGAGAG 
CCTGGACCTG ACATCACAGG ACCCAAAGGT CGTGATGGCC AACGAGGGGA ACAAGGACCC 
CCTGGTGATC CTGGACTTCC GGGACCTTCA AATGTCATAA GCCGTTTGGG ACTCAAAGGA 
CTCAAGGGTG ATAAAGGTCT AATAGGCCCT GAGGGTCTGC GTGGGGATCC TGGGAACCAA 
GGAGAGCAAG GAGATCTGTG CAGGCTATGT GCTGCTTCTG CTGCTGATCC TGGTCCTCCA 
GGACAACCTG GGGATTCAGG CGAACCCGGC AAACCAGGTG CTGTTGGAAT TCAAGGTAAT 
AAAGGATCCA GAGGCTTGAA AGGTCCAATT GGTCCAGCTG GTCCTCCGGG TTTCTCAGGT 
CTTACAGGTT TTCCAGGGCC TTCAGGCCCC AAGGGAGAAA TGGGGATGAA ATATGAGATT 
GGGGAAAAAG GCTTTAAAGG AGACACTGGT GCAGTAGGAC TATTGGGATT AAGTGGACAA 
GATGGTTTAC CAGGCATCCC TGGCTTCAAA GGGCCACCGG GAGAAAAAGG AAAGCCAAGT 
GCTTTAGCTC CTAAAGGCGA TCAAGGATAT CCAGGTGTTG GTGGGGAACA AGGTAGCACA 
GGTCCAATGG GAAGTCAAGG GTCAATGGGT GTTGGGTCAC CAGGGGCACC TGGAGAAAAA 
GGAAATTTGG GAGTAGTGGG CCGGCAAGGA AATCTTGGAG ATCCAGGTGA AAAGGGATCT 
CCAGGACCAG TACAAACAAT ACCTGGAGAC CCAGGAGAGC CTGGACTGCC TGGTTCACCT 
GGTCTACCAG GACCTCCAGG TAATGAAGGT TGTGCTGGGG TTCAAGGATT CCCAGGATTG 
TGTGGGTTAA AAGGAGAGAA AGGACTCTCA ACACCAGGAG ATACTGGACT TACTGGTCCA 
AAAGGTGATA AGGGTGTGAA AGGGAGGCAA GGTTCACCTG GTTGTGGAAG TATAGGCCGT 
CCTGGACCTC CAGGGCCACC TGGAATGCCT GGCCCAAAGG GTGAAATAGC ATTTGGCTGC 
CCAGGGGCAA GGGGTAGAGA AGGTCTGCCA GGAGATGAAG GTTGCCTTGG TCCACCAGGA 
ATCTCTGGTC CTCCTGGATT ATCTGGATGT CGAGGTCAAA AAGGACAGGA TGGACCACCT 
GGAGAATTTG GTGTGCAGGG TTTTGATGGT TTTCCTGGAG TGGCTGGTGT TCCAGGATGC 
TGTATATCAG GGGCCAAAGG AGATGAAGGT CCCTCAGGAA TCAGAGGAGC AGATGGACCC 
CCTGGTTTGC CAGGTTTGAA AGGGCCACAA GGTGAAACTG GACTACCTGG ATTGAGCATA 
CCTGGTGTAA CAGGTGTGGT TGGGATACCT GGGATCTTTG GACCAGTTGG TGCAGTTGGT 
TTTCGGGGAC CAAAAGGTTT CCAGGGTCGA GATGGTTCCC CAGGATTTCC AGGTATACGG 
GGAGATCAGG GCAGTCCTGG AATGACTGGC CCACCTGGAG TAGATGGAAA TCCTGGACCA 
CCAGGGCTCC GGGGACCTAA AGGTTTAATG GGAAGGAATG GGGTCCCAGG TGATGAAGGA 
AAGCCTGGGG AGCCAGGAGA CCCTGGGCCC CGTGGCCCAA AGGGACAATC ATCAAATGTT 
ACGTCTCCAG GAGAACTTGG TCCTCCTGGC CTACCAGGTC TCCTTGGTCT GCCTGGTAGT 
AAAGGGGAAA TAGGACTTCC AGGCATTTGT GGGCAATGTG GGAAGAATGG CGTTCCAGGC 
AGTTCTGGTC CACCAGGCCC TCCTGGTGAC CCTGGCATAA AGGGGCTTCC TGGAAATCAG 
GGTCTTAATG GAAACTTTGG AGATCAAGGA GTTATGGGGT TTCCAGGAGT GAAAGGTGTT 
AAAGGTTCAC CTGGTTTTCA CGGGGAGCCT GGGATTCCTG GTGTTCCAGG ACCAAATGGA 
CCAAAGGGGA TCAAAGGTGA TCCTAGCCCC CCTGGGCCAG AGACTCCAGG ACTACTTGGT 
GAGAAGGGTG AACCAGGGTG TGCTGGGGGT ACAGGGAAGA AAGGTGACAA AGGTATATGT 
GGAATAAATG GGATCCCAGG TTTCTCTGGA GTACCTGGGG AAAGAGGAGT AACTGGAGTG 
ACAGGAGATC CAGGACCCCC AGGAGAAGCT GGTGATGATG GGTTTGATGG ATTTCCAGGT 
CCACCTGGAT GTGAAGGACC CTGTGGTCCA CCAGGTGTTC CTGGCTCGCC TGGAGATCCT 
GGACCTCCAG GACCTCGGGG AGAACAAGGC AGAGATGGCA TTCCAGGGTC AGCCGGAGAA 
AAAGGAGACA GAGGGTGCAC TATGACTGGG GATTCTGGCC TACCTGGCAT CAAGGGTGAG 
AAAGGCATTA AAGGCACCCG TGGACCACCA GGGCCATGTG GGCCACCAGG ACCCTGTGGC 
CCATCTGGAG AAATTGGTTC TCCAGGCAAA CCCGGAGAAA AAGGGCAACG TGGGTGTTCT 
GGACCACCTG GTGTCTGTAT TAGTGGCCCA AAAGGAGAGC GAGGACCTAA TGGGTTTCCT 
GGTCCACCAG GCCTCCAAGG ACCATGTGGG CCTCCTGGAG ACCCAGGTCC AGGCCTTAAG 
GGAGATAAAG GCTGTGTCGG GCCCCAAGGA CGCGTAGGAC CACCTGGTTG TACTGGTGAC 
CGGGGAACTC CAGGTGAACC AGGTAAAGAT GGAGATTGTG GTTTAAGGGG TCCAAAAGGA 
AATATAGGGC CTTTGGGTAG AGTTGGACCA GATGGACAAA AAGGACAGTG TGGTCCTGTT 
GGACCTGCTG GATTAAAAGG TTGTAAAGGA TCTCCTGGAA CTAAAGGGCC AAAAGGCTGT 
GATTACACTG TTGAGATCTT TTCTACACCA GGAGATCGCG GATTTCGTGG AAAAACCGGT 
CAGAAAGGAG AACCAGGGGA TAATGGAGAT AAAGGATTGC GAGGTCTAAA AGGAACAAAA 
GGTCAAAAAG GAGATATAGG AGCAAATGGA CCTGATGGAT CTAAGGGGAT GCTCGGGCTG 
CAGGGAGACA GTGGAGAAAA AGGACTAAAA GGTGCTCGGG GTTTGACAGG CGAAGTGGGT 
GCCAAGGGTG CACCAGGTGC AAAAGGTCCA CAACTGAGAA AAAGAGACGG ATTCCTTTTC 
ACCAGACATA GTCAGACAAC GGTTATACCA GAATGTCCTG CGGGGTCGAA ACGTTTATAC 
ACTGGATATT CACTTCTCTT CATCAACGGC AATAACCGAG GACATGGACA AGACCTAGGA 
ACTCTGGGAA GTTGTTTGCC GATGTTCAAC ACCATGCCTT TTATGGTGTG TAACCGTGAT 
GAAACTTGTC GCTACGCCTC TCGAAATGAC TACTCCTATT GGCTGTCAAC AGACACACCA 
ATGTTACCTG ACCAGCAAAT GATGTCTGGT GAAATACTGA AATGGTACAT CAGCAGGTGT 
TCCGTATGCG AGGCAATAGC TAATGTTATA GCAATTCACA GTCAGACCAT TAACATCCCA 
CAATGCCCTG TTGGGTGGCT TTCACTATGG GAAGGTTACT CCTTTGTAAT GCAAACAGGA 
GTTGGCGCAG AAGGTTCTGG GCAACCATTG GTCTCTCCTG GATCCTGTCT GGAACAATTC 
CGGAAGATTC CATTCATAGA GTGTCATGGT CGCGGCACCT GCAACTTCTA TCCTGATTCC 
TACAGCTACT GGCTTGCTTC ACTCGACCAC ACCAATATGT TCAGTATGCC AAACCGCCAA 
ACAGCAAAAC AGAAAGAAAT CATCAGCCGC TGTCAGGTCT GTATGAAGCA ACAGCCTCAA 
GGTGACCTTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_694013.8
CDS	22..5016
Translation	MDHWVLLRVTLVGMMLLGLLTPTQSKGACDCSGKSGCDCQGVKGQKGEKGSLGLVGDVGVQGHQGKEGPPGFPGLKGYLGIPGIPGRKGSRGISGTPGFFGPPGLPGLPGIQGPRGPPGLPGCNGTKGEPGPPGLIGPMGRPGIQGRPGIQGPKGETIFIAAAEQMIGLTGQQGRAGDPGPPGLPGVQGNIGPEGPKGNPGPQGIPGFPGEKGKEGRSMGIPGPSGQKGERGPPGIPGQKGIKLFASGPKEPKGDTGDSGDRGSPGIIGLRGRPGPKGEKGERGDPGGPGKPGKEGLQGELGSKGGKGDKGYPGDTGIEGEKGQKGNPGPPGTPGEFIVINTEVKGQKGDPGPPGLLGLRGSEGFYGFPGYQGLPGTPIQGPNGKPGLPGFKGLKGDKGEPGPDITGPKGRDGQRGEQGPPGDPGLPGPSNVISRLGLKGLKGDKGLIGPEGLRGDPGNQGEQGDLCRLCAASAADPGPPGQPGDSGEPGKPGAVGIQGNKGSRGLKGPIGPAGPPGFSGLTGFPGPSGPKGEMGMKYEIGEKGFKGDTGAVGLLGLSGQDGLPGIPGFKGPPGEKGKPSALAPKGDQGYPGVGGEQGSTGPMGSQGSMGVGSPGAPGEKGNLGVVGRQGNLGDPGEKGSPGPVQTIPGDPGEPGLPGSPGLPGPPGNEGCAGVQGFPGLCGLKGEKGLSTPGDTGLTGPKGDKGVKGRQGSPGCGSIGRPGPPGPPGMPGPKGEIAFGCPGARGREGLPGDEGCLGPPGISGPPGLSGCRGQKGQDGPPGEFGVQGFDGFPGVAGVPGCCISGAKGDEGPSGIRGADGPPGLPGLKGPQGETGLPGLSIPGVTGVVGIPGIFGPVGAVGFRGPKGFQGRDGSPGFPGIRGDQGSPGMTGPPGVDGNPGPPGLRGPKGLMGRNGVPGDEGKPGEPGDPGPRGPKGQSSNVTSPGELGPPGLPGLLGLPGSKGEIGLPGICGQCGKNGVPGSSGPPGPPGDPGIKGLPGNQGLNGNFGDQGVMGFPGVKGVKGSPGFHGEPGIPGVPGPNGPKGIKGDPSPPGPETPGLLGEKGEPGCAGGTGKKGDKGICGINGIPGFSGVPGERGVTGVTGDPGPPGEAGDDGFDGFPGPPGCEGPCGPPGVPGSPGDPGPPGPRGEQGRDGIPGSAGEKGDRGCTMTGDSGLPGIKGEKGIKGTRGPPGPCGPPGPCGPSGEIGSPGKPGEKGQRGCSGPPGVCISGPKGERGPNGFPGPPGLQGPCGPPGDPGPGLKGDKGCVGPQGRVGPPGCTGDRGTPGEPGKDGDCGLRGPKGNIGPLGRVGPDGQKGQCGPVGPAGLKGCKGSPGTKGPKGCDYTVEIFSTPGDRGFRGKTGQKGEPGDNGDKGLRGLKGTKGQKGDIGANGPDGSKGMLGLQGDSGEKGLKGARGLTGEVGAKGAPGAKGPQLRKRDGFLFTRHSQTTVIPECPAGSKRLYTGYSLLFINGNNRGHGQDLGTLGSCLPMFNTMPFMVCNRDETCRYASRNDYSYWLSTDTPMLPDQQMMSGEILKWYISRCSVCEAIANVIAIHSQTINIPQCPVGWLSLWEGYSFVMQTGVGAEGSGQPLVSPGSCLEQFRKIPFIECHGRGTCNFYPDSYSYWLASLDHTNMFSMPNRQTAKQKEIISRCQVCMKQQPQGDLA

Target ORF information:

RefSeq Version	XM_688921.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type IV, alpha 3 (col4a3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_688921.8

ORF Insert Sequence:

ATGGATCATT GGGTATTATT GCGCGTGACA CTTGTCGGAA TGATGCTGCT GGGACTTCTG 
ACACCGACGC AAAGTAAAGG GGCTTGTGAT TGCAGTGGAA AATCAGGATG TGACTGCCAA 
GGAGTCAAAG GACAAAAGGG TGAGAAGGGT TCTCTTGGTT TGGTTGGTGA TGTTGGAGTT 
CAGGGGCATC AAGGAAAGGA AGGCCCTCCT GGATTTCCTG GTCTGAAGGG TTATTTGGGT 
ATACCAGGCA TCCCGGGACG TAAAGGTTCT AGGGGAATTT CAGGGACTCC TGGATTCTTT 
GGCCCTCCGG GACTACCGGG GCTTCCTGGG ATTCAGGGAC CAAGGGGGCC TCCTGGTCTC 
CCAGGATGCA ATGGAACAAA GGGTGAGCCT GGTCCTCCAG GTTTAATTGG GCCAATGGGG 
CGTCCTGGAA TCCAGGGAAG GCCAGGAATC CAAGGACCTA AGGGGGAGAC CATTTTTATT 
GCCGCTGCCG AACAAATGAT AGGACTTACG GGTCAACAAG GCAGAGCTGG AGATCCAGGT 
CCACCTGGGT TGCCAGGTGT TCAGGGAAAT ATTGGACCAG AGGGGCCTAA AGGTAACCCT 
GGACCACAAG GCATTCCTGG ATTTCCAGGA GAGAAGGGTA AAGAAGGTCG GAGCATGGGA 
ATACCTGGAC CCAGCGGTCA AAAGGGGGAG AGAGGACCCC CTGGTATACC TGGACAAAAA 
GGCATAAAGT TATTTGCATC AGGACCAAAG GAGCCTAAGG GTGACACTGG GGATTCTGGA 
GACAGAGGCA GCCCTGGAAT AATTGGTCTA CGAGGTCGTC CCGGTCCTAA GGGGGAGAAA 
GGGGAAAGAG GAGACCCTGG GGGTCCGGGT AAACCTGGAA AAGAGGGGCT ACAAGGAGAG 
TTAGGCTCTA AGGGAGGGAA GGGAGATAAA GGCTATCCCG GAGATACAGG CATAGAAGGG 
GAGAAGGGTC AGAAGGGTAA CCCTGGCCCC CCTGGTACGC CTGGAGAGTT CATTGTAATA 
AATACAGAGG TCAAAGGTCA AAAAGGGGAT CCTGGGCCAC CAGGACTTCT TGGTTTACGA 
GGTAGTGAAG GTTTCTATGG TTTTCCTGGA TATCAAGGAC TTCCAGGGAC CCCCATACAA 
GGACCTAATG GAAAACCAGG TCTACCAGGG TTTAAAGGTT TGAAAGGGGA TAAAGGAGAG 
CCTGGACCTG ACATCACAGG ACCCAAAGGT CGTGATGGCC AACGAGGGGA ACAAGGACCC 
CCTGGTGATC CTGGACTTCC GGGACCTTCA AATGTCATAA GCCGTTTGGG ACTCAAAGGA 
CTCAAGGGTG ATAAAGGTCT AATAGGCCCT GAGGGTCTGC GTGGGGATCC TGGGAACCAA 
GGAGAGCAAG GAGATCTGTG CAGGCTATGT GCTGCTTCTG CTGCTGATCC TGGTCCTCCA 
GGACAACCTG GGGATTCAGG CGAACCCGGC AAACCAGGTG CTGTTGGAAT TCAAGGTAAT 
AAAGGATCCA GAGGCTTGAA AGGTCCAATT GGTCCAGCTG GTCCTCCGGG TTTCTCAGGT 
CTTACAGGTT TTCCAGGGCC TTCAGGCCCC AAGGGAGAAA TGGGGATGAA ATATGAGATT 
GGGGAAAAAG GCTTTAAAGG AGACACTGGT GCAGTAGGAC TATTGGGATT AAGTGGACAA 
GATGGTTTAC CAGGCATCCC TGGCTTCAAA GGGCCACCGG GAGAAAAAGG AAAGCCAAGT 
GCTTTAGCTC CTAAAGGCGA TCAAGGATAT CCAGGTGTTG GTGGGGAACA AGGTAGCACA 
GGTCCAATGG GAAGTCAAGG GTCAATGGGT GTTGGGTCAC CAGGGGCACC TGGAGAAAAA 
GGAAATTTGG GAGTAGTGGG CCGGCAAGGA AATCTTGGAG ATCCAGGTGA AAAGGGATCT 
CCAGGACCAG TACAAACAAT ACCTGGAGAC CCAGGAGAGC CTGGACTGCC TGGTTCACCT 
GGTCTACCAG GACCTCCAGG TAATGAAGGT TGTGCTGGGG TTCAAGGATT CCCAGGATTG 
TGTGGGTTAA AAGGAGAGAA AGGACTCTCA ACACCAGGAG ATACTGGACT TACTGGTCCA 
AAAGGTGATA AGGGTGTGAA AGGGAGGCAA GGTTCACCTG GTTGTGGAAG TATAGGCCGT 
CCTGGACCTC CAGGGCCACC TGGAATGCCT GGCCCAAAGG GTGAAATAGC ATTTGGCTGC 
CCAGGGGCAA GGGGTAGAGA AGGTCTGCCA GGAGATGAAG GTTGCCTTGG TCCACCAGGA 
ATCTCTGGTC CTCCTGGATT ATCTGGATGT CGAGGTCAAA AAGGACAGGA TGGACCACCT 
GGAGAATTTG GTGTGCAGGG TTTTGATGGT TTTCCTGGAG TGGCTGGTGT TCCAGGATGC 
TGTATATCAG GGGCCAAAGG AGATGAAGGT CCCTCAGGAA TCAGAGGAGC AGATGGACCC 
CCTGGTTTGC CAGGTTTGAA AGGGCCACAA GGTGAAACTG GACTACCTGG ATTGAGCATA 
CCTGGTGTAA CAGGTGTGGT TGGGATACCT GGGATCTTTG GACCAGTTGG TGCAGTTGGT 
TTTCGGGGAC CAAAAGGTTT CCAGGGTCGA GATGGTTCCC CAGGATTTCC AGGTATACGG 
GGAGATCAGG GCAGTCCTGG AATGACTGGC CCACCTGGAG TAGATGGAAA TCCTGGACCA 
CCAGGGCTCC GGGGACCTAA AGGTTTAATG GGAAGGAATG GGGTCCCAGG TGATGAAGGA 
AAGCCTGGGG AGCCAGGAGA CCCTGGGCCC CGTGGCCCAA AGGGACAATC ATCAAATGTT 
ACGTCTCCAG GAGAACTTGG TCCTCCTGGC CTACCAGGTC TCCTTGGTCT GCCTGGTAGT 
AAAGGGGAAA TAGGACTTCC AGGCATTTGT GGGCAATGTG GGAAGAATGG CGTTCCAGGC 
AGTTCTGGTC CACCAGGCCC TCCTGGTGAC CCTGGCATAA AGGGGCTTCC TGGAAATCAG 
GGTCTTAATG GAAACTTTGG AGATCAAGGA GTTATGGGGT TTCCAGGAGT GAAAGGTGTT 
AAAGGTTCAC CTGGTTTTCA CGGGGAGCCT GGGATTCCTG GTGTTCCAGG ACCAAATGGA 
CCAAAGGGGA TCAAAGGTGA TCCTAGCCCC CCTGGGCCAG AGACTCCAGG ACTACTTGGT 
GAGAAGGGTG AACCAGGGTG TGCTGGGGGT ACAGGGAAGA AAGGTGACAA AGGTATATGT 
GGAATAAATG GGATCCCAGG TTTCTCTGGA GTACCTGGGG AAAGAGGAGT AACTGGAGTG 
ACAGGAGATC CAGGACCCCC AGGAGAAGCT GGTGATGATG GGTTTGATGG ATTTCCAGGT 
CCACCTGGAT GTGAAGGACC CTGTGGTCCA CCAGGTGTTC CTGGCTCGCC TGGAGATCCT 
GGACCTCCAG GACCTCGGGG AGAACAAGGC AGAGATGGCA TTCCAGGGTC AGCCGGAGAA 
AAAGGAGACA GAGGGTGCAC TATGACTGGG GATTCTGGCC TACCTGGCAT CAAGGGTGAG 
AAAGGCATTA AAGGCACCCG TGGACCACCA GGGCCATGTG GGCCACCAGG ACCCTGTGGC 
CCATCTGGAG AAATTGGTTC TCCAGGCAAA CCCGGAGAAA AAGGGCAACG TGGGTGTTCT 
GGACCACCTG GTGTCTGTAT TAGTGGCCCA AAAGGAGAGC GAGGACCTAA TGGGTTTCCT 
GGTCCACCAG GCCTCCAAGG ACCATGTGGG CCTCCTGGAG ACCCAGGTCC AGGCCTTAAG 
GGAGATAAAG GCTGTGTCGG GCCCCAAGGA CGCGTAGGAC CACCTGGTTG TACTGGTGAC 
CGGGGAACTC CAGGTGAACC AGGTAAAGAT GGAGATTGTG GTTTAAGGGG TCCAAAAGGA 
AATATAGGGC CTTTGGGTAG AGTTGGACCA GATGGACAAA AAGGACAGTG TGGTCCTGTT 
GGACCTGCTG GATTAAAAGG TTGTAAAGGA TCTCCTGGAA CTAAAGGGCC AAAAGGCTGT 
GATTACACTG TTGAGATCTT TTCTACACCA GGAGATCGCG GATTTCGTGG AAAAACCGGT 
CAGAAAGGAG AACCAGGGGA TAATGGAGAT AAAGGATTGC GAGGTCTAAA AGGAACAAAA 
GGTCAAAAAG GAGATATAGG AGCAAATGGA CCTGATGGAT CTAAGGGGAT GCTCGGGCTG 
CAGGGAGACA GTGGAGAAAA AGGACTAAAA GGTGCTCGGG GTTTGACAGG CGAAGTGGGT 
GCCAAGGGTG CACCAGGTGC AAAAGGTCCA CAACTGAGAA AAAGAGACGG ATTCCTTTTC 
ACCAGACATA GTCAGACAAC GGTTATACCA GAATGTCCTG CGGGGTCGAA ACGTTTATAC 
ACTGGATATT CACTTCTCTT CATCAACGGC AATAACCGAG GACATGGACA AGACCTAGGA 
ACTCTGGGAA GTTGTTTGCC GATGTTCAAC ACCATGCCTT TTATGGTGTG TAACCGTGAT 
GAAACTTGTC GCTACGCCTC TCGAAATGAC TACTCCTATT GGCTGTCAAC AGACACACCA 
ATGTTACCTG ACCAGCAAAT GATGTCTGGT GAAATACTGA AATGGTACAT CAGCAGGTGT 
TCCGTATGCG AGGCAATAGC TAATGTTATA GCAATTCACA GTCAGACCAT TAACATCCCA 
CAATGCCCTG TTGGGTGGCT TTCACTATGG GAAGGTTACT CCTTTGTAAT GCAAACAGGA 
GTTGGCGCAG AAGGTTCTGG GCAACCATTG GTCTCTCCTG GATCCTGTCT GGAACAATTC 
CGGAAGATTC CATTCATAGA GTGTCATGGT CGCGGCACCT GCAACTTCTA TCCTGATTCC 
TACAGCTACT GGCTTGCTTC ACTCGACCAC ACCAATATGT TCAGTATGCC AAACCGCCAA 
ACAGCAAAAC AGAAAGAAAT CATCAGCCGC TGTCAGGTCT GTATGAAGCA ACAGCCTCAA 
GGTGACCTTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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COL4A3 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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