alg9 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	alg9
Entrez Gene ID	393598
Full Name	ALG9, alpha-1,2-mannosyltransferase
Synonyms	zgc:63820
General protein information	Preferred Names ALG9, alpha-1,2-mannosyltransferase Names alpha-1,2-mannosyltransferase ALG9 asparagine-linked glycosylation 9 homolog (S. cerevisiae, alpha- 1,2-mannosyltransferase)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200625.2	NP_956919.2	alpha-1,2-mannosyltransferase ALG9
XM_009301841.2	XP_009300116.1	alpha-1,2-mannosyltransferase ALG9 isoform X1
XM_009301841.3	XP_009300116.1	alpha-1,2-mannosyltransferase ALG9 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	alg9	NP_956919.1
Caenorhabditis elegans (roundworm)	C14A4.3	NP_496282.2
Canis lupus familiaris (dog)	ALG9	XP_850797.2
Gallus gallus (chicken)	ALG9	XP_004948213.1
Drosophila melanogaster (fruit fly)	CG11851	NP_651353.1
Arabidopsis thaliana (thale cress)	AT1G16900	NP_173134.2
Kluyveromyces lactis	KLLA0D19404g	XP_453923.1
Oryza sativa (rice)	Os01g0209000	NP_001042360.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003601	XP_313360.3
Pan troglodytes (chimpanzee)	ALG9	XP_003313338.1
Macaca mulatta (Rhesus monkey)	ALG9	XP_001106241.2
Bos taurus (cattle)	ALG9	XP_005215938.1
Mus musculus (house mouse)	Alg9	NP_598742.1
Eremothecium gossypii	AGOS_ABL185C	NP_982762.1
Homo sapiens (human)	ALG9	NP_079016.2
Saccharomyces cerevisiae (baker's yeast)	ALG9	NP_014180.1
Xenopus tropicalis (tropical clawed frog)	alg9	XP_004916165.1
Rattus norvegicus (Norway rat)	Alg9	NP_001102470.1
Schizosaccharomyces pombe (fission yeast)	alg9	NP_594684.1
Magnaporthe oryzae (rice blast fungus)	MGG_14835	XP_003717392.1
Neurospora crassa	NCU04454	XP_955912.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

trappc11 is required for protein glycosylation in zebrafish and humans.
DeRossi C, Vacaru A, Rafiq R, Cinaroglu A, Imrie D, Nayar S, Baryshnikova A, Milev MP, Stanga D, Kadakia D, Gao N, Chu J, Freeze HH, Lehrman MA, Sacher M, Sadler KC
Molecular biology of the cell27(8)1220-34(2016 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish miR-1 and miR-133 shape muscle gene expression and regulate sarcomeric actin organization.
Mishima Y, Abreu-Goodger C, Staton AA, Stahlhut C, Shou C, Cheng C, Gerstein M, Enright AJ, Giraldez AJ
Genes & development23(5)619-32(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following alg9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the alg9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa40477	NM_200625.2 Latest version!	Danio rerio ALG9 alpha-1,2-mannosyltransferase (alg9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa40476	XM_009301841.3 Latest version!	Danio rerio ALG9, alpha-1,2-mannosyltransferase (alg9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
ODa40476	XM_009301841.2	Danio rerio ALG9, alpha-1,2-mannosyltransferase (alg9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

alg9 ( NM_200625.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956919.2 Danio rerio ALG9 alpha-1,2-mannosyltransferase (alg9), mRNA.

Price & Availability↑

CloneID	ODa40477
Clone ID Related Accession (Same CDS sequence)	NM_200625.2
Accession Version	NM_200625.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554566400000
Organism	Danio rerio(zebrafish)
Product	alpha-1,2-mannosyltransferase ALG9
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX901896.7, EH592472.1 and EH609977.1. On or before Jun 12, 2015 this sequence version replaced XM_009301842.1, NM_200625.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC056834.1, GDQH01017132.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGA AGAGTCTCCG TCAGCGCAGC AGACGGGGGA GCAAACAGGA CGCCAACAAC 
GTGAACAACA CACCTACCCC TGAAAACCGA GCCACAAAAG ACGACAAATG TGCTGAGGAC 
AATAAGTCCA GTGACCCCCG AAATGAGTCA TCCGGTAAGG CAGGTCAGGT TTGGGCTCCT 
GAGGGCTCCA CAGCCTTCAA GTGCCTGGTT TCAGCCCGCT TCTGTGCCGC ACTGCTCAGT 
AACCTCTCTG ACTGTGATGA AACCTTCAAT TACTGGGAGC CAACTCACTA CTTGCTCTAT 
GGAACAGGGA TGCAGACATG GGAGTATTCC CCATCCTACG CCATTCGTTC CTATGCGTAT 
CTCTGGCTCC ATGCCCTTCC TGCCTGCTTT CATGCCAAAA TATTACAGAC AAACAAGGTC 
CTTGTGTTTT ATTTCCTGCG CTGCATGTTA GCGTTTGGCT GTTGTGTGTG TGAGCTCTAC 
TTCTATAAGG CTGTGTGTAA GAAATTTGGC CTACATGTGG GACGGCTTCT GCTGGCGTTC 
CTCGTCCTGA GTGCAGGGAT GTTCTGCTCC TCTGCAGCTT TCCTGCCAAG TACTTTTTGC 
ATGTACAGTA CAGTGGTAGC TATGACTGGC TGGTTTCAGG GGCGTCCGAG TCTGGCTGTG 
CTGGGTGTCG CAGCAGGGGC CATTGTGGGC TGGCCCTTCA GTGCTCTGCT TGGGATACCC 
ATTGCCTTTG ACCTTCTGTG GCTGAAGAAG AAATGGAAGA GTTTTATCAT GTGGACACTA 
GTGGCATTGC TGCTCTTTCT GGTCCCTGTG GTTATAGTAG ACAGCTACTA CTATGGCAAG 
CTGGTGATTG CTCCTCTGAA TATAATACTG TACAATGTAT TTACTCCTCA TGGGCCAGAT 
CTCTATGGCA CAGAGCCCTG GCATTACTAC TTTGTGAATG GTTTTCTTAA TTTCAACATT 
GTGTTTGTGT TGGCCCTGTT TTCTCTTCCT CTCACGGCGC TTATGGAGGC ACTGCTTCAA 
AGATTCAATG TTCAGAACCT CGGCCGGCCG TACTGGTTAA CTTTGTCTCC AATGTACCTG 
TGGATGCTGG TCTTCTTCAC TCGCCCGCAT AAAGAGGAAC GCTTTCTGTT TCCCATCTAC 
CCGCTCATCT GTCTGTGCGG GGCAGTGGCG CTCTCCTCAT TGCAGAAGTG CTATCACTTC 
ATTTTTCAAC GCTATCGTCT GGAGCACTAC ACTATCTCCT CCAACTGGCT GGCTCTGGGT 
TCTGTGCTGC TGTTTGGAGT GCTCTCGCTG TCTCGATCTG TGGCCCTATT CAGAGGCTAT 
CATGCTCCGC TGGATTTGTA CCCAGAGTTT CATCGCATTG CTAAAGATCC TGCAATCCAC 
ACCGTTCCAG AGGGAAGACT GGTCAATGTT TGTGTCGGGA AAGAGTGGTA CCGCTTTCCC 
AGCAGCTTTT TCCTGCCTAA CAACTGGCAC CTACAGTTTA TCCAGTCAGA ATTCAGGGGT 
CAGTTACCTA AGCCATATGC CATGGGTCCT GATGCCACCT GGATCATCCC ACCAGACATG 
AATGATCAGA ATCTTGAGGA GACATCACGA TACGTAGATT TGAAGCAGTG TCATTACCTG 
GTGGATCTGG CAACAGAAAC TGAAGCCCCT CGTGAACCAC GATATGCCGC GAACAAAGAG 
GAGTGGAGCG TTATTGCTGA CAAGCCTTTT CTAGACTCTA CCAGGTCCTC CAGAATTCTC 
AGGGCCTTTT TTATCCCGTT TCTGTCAGAG CAATACAACA CCTACAGCAG ATACGTCATC 
CTCAAACCGA GGCGACCCAA ACACACGCGG AAGAGACCGC AGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956919.2
CDS	26..1873
Translation	MATKSLRQRSRRGSKQDANNVNNTPTPENRATKDDKCAEDNKSSDPRNESSGKAGQVWAPEGSTAFKCLVSARFCAALLSNLSDCDETFNYWEPTHYLLYGTGMQTWEYSPSYAIRSYAYLWLHALPACFHAKILQTNKVLVFYFLRCMLAFGCCVCELYFYKAVCKKFGLHVGRLLLAFLVLSAGMFCSSAAFLPSTFCMYSTVVAMTGWFQGRPSLAVLGVAAGAIVGWPFSALLGIPIAFDLLWLKKKWKSFIMWTLVALLLFLVPVVIVDSYYYGKLVIAPLNIILYNVFTPHGPDLYGTEPWHYYFVNGFLNFNIVFVLALFSLPLTALMEALLQRFNVQNLGRPYWLTLSPMYLWMLVFFTRPHKEERFLFPIYPLICLCGAVALSSLQKCYHFIFQRYRLEHYTISSNWLALGSVLLFGVLSLSRSVALFRGYHAPLDLYPEFHRIAKDPAIHTVPEGRLVNVCVGKEWYRFPSSFFLPNNWHLQFIQSEFRGQLPKPYAMGPDATWIIPPDMNDQNLEETSRYVDLKQCHYLVDLATETEAPREPRYAANKEEWSVIADKPFLDSTRSSRILRAFFIPFLSEQYNTYSRYVILKPRRPKHTRKRPQA

Target ORF information:

RefSeq Version	NM_200625.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ALG9 alpha-1,2-mannosyltransferase (alg9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200625.2

ORF Insert Sequence:

ATGGCGACGA AGAGTCTCCG TCAGCGCAGC AGACGGGGGA GCAAACAGGA CGCCAACAAC 
GTGAACAACA CACCTACCCC TGAAAACCGA GCCACAAAAG ACGACAAATG TGCTGAGGAC 
AATAAGTCCA GTGACCCCCG AAATGAGTCA TCCGGTAAGG CAGGTCAGGT TTGGGCTCCT 
GAGGGCTCCA CAGCCTTCAA GTGCCTGGTT TCAGCCCGCT TCTGTGCCGC ACTGCTCAGT 
AACCTCTCTG ACTGTGATGA AACCTTCAAT TACTGGGAGC CAACTCACTA CTTGCTCTAT 
GGAACAGGGA TGCAGACATG GGAGTATTCC CCATCCTACG CCATTCGTTC CTATGCGTAT 
CTCTGGCTCC ATGCCCTTCC TGCCTGCTTT CATGCCAAAA TATTACAGAC AAACAAGGTC 
CTTGTGTTTT ATTTCCTGCG CTGCATGTTA GCGTTTGGCT GTTGTGTGTG TGAGCTCTAC 
TTCTATAAGG CTGTGTGTAA GAAATTTGGC CTACATGTGG GACGGCTTCT GCTGGCGTTC 
CTCGTCCTGA GTGCAGGGAT GTTCTGCTCC TCTGCAGCTT TCCTGCCAAG TACTTTTTGC 
ATGTACAGTA CAGTGGTAGC TATGACTGGC TGGTTTCAGG GGCGTCCGAG TCTGGCTGTG 
CTGGGTGTCG CAGCAGGGGC CATTGTGGGC TGGCCCTTCA GTGCTCTGCT TGGGATACCC 
ATTGCCTTTG ACCTTCTGTG GCTGAAGAAG AAATGGAAGA GTTTTATCAT GTGGACACTA 
GTGGCATTGC TGCTCTTTCT GGTCCCTGTG GTTATAGTAG ACAGCTACTA CTATGGCAAG 
CTGGTGATTG CTCCTCTGAA TATAATACTG TACAATGTAT TTACTCCTCA TGGGCCAGAT 
CTCTATGGCA CAGAGCCCTG GCATTACTAC TTTGTGAATG GTTTTCTTAA TTTCAACATT 
GTGTTTGTGT TGGCCCTGTT TTCTCTTCCT CTCACGGCGC TTATGGAGGC ACTGCTTCAA 
AGATTCAATG TTCAGAACCT CGGCCGGCCG TACTGGTTAA CTTTGTCTCC AATGTACCTG 
TGGATGCTGG TCTTCTTCAC TCGCCCGCAT AAAGAGGAAC GCTTTCTGTT TCCCATCTAC 
CCGCTCATCT GTCTGTGCGG GGCAGTGGCG CTCTCCTCAT TGCAGAAGTG CTATCACTTC 
ATTTTTCAAC GCTATCGTCT GGAGCACTAC ACTATCTCCT CCAACTGGCT GGCTCTGGGT 
TCTGTGCTGC TGTTTGGAGT GCTCTCGCTG TCTCGATCTG TGGCCCTATT CAGAGGCTAT 
CATGCTCCGC TGGATTTGTA CCCAGAGTTT CATCGCATTG CTAAAGATCC TGCAATCCAC 
ACCGTTCCAG AGGGAAGACT GGTCAATGTT TGTGTCGGGA AAGAGTGGTA CCGCTTTCCC 
AGCAGCTTTT TCCTGCCTAA CAACTGGCAC CTACAGTTTA TCCAGTCAGA ATTCAGGGGT 
CAGTTACCTA AGCCATATGC CATGGGTCCT GATGCCACCT GGATCATCCC ACCAGACATG 
AATGATCAGA ATCTTGAGGA GACATCACGA TACGTAGATT TGAAGCAGTG TCATTACCTG 
GTGGATCTGG CAACAGAAAC TGAAGCCCCT CGTGAACCAC GATATGCCGC GAACAAAGAG 
GAGTGGAGCG TTATTGCTGA CAAGCCTTTT CTAGACTCTA CCAGGTCCTC CAGAATTCTC 
AGGGCCTTTT TTATCCCGTT TCTGTCAGAG CAATACAACA CCTACAGCAG ATACGTCATC 
CTCAAACCGA GGCGACCCAA ACACACGCGG AAGAGACCGC AGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

alg9 ( XM_009301841.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300116.1 Danio rerio ALG9, alpha-1,2-mannosyltransferase (alg9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40476
Clone ID Related Accession (Same CDS sequence)	XM_009301841.2 , XM_009301841.3
Accession Version	XM_009301841.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	alpha-1,2-mannosyltransferase ALG9 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301841.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCAC GCACAGCCAG TAGAAACATG GCGACGAAGA GTCTCCGTCA GCGCAGCAGA 
CGGGGGAGCA AACAGGACGC CAACAACGTG AACAACACAC CTACCCCTGA AAACCGAGCC 
ACAAAAGACG ACAAATGTGC TGAGGACAAT AAGTCCAGTG ACCCCCGAAA TGATTTGCTC 
CCAGATAAGC ACATTAGCTC CTGTATCCCG CCTCTCTCTT ACAGGTCATC CGGTAAGGCA 
GGTCAGGTTT GGGCTCCTGA GGGCTCCACA GCCTTCAAGT GCCTGGTTTC AGCCCGCTTC 
TGTGCCGCAC TGCTCAGTAA CCTCTCTGAC TGTGATGAAA CCTTCAATTA CTGGGAGCCA 
ACTCACTACT TGCTCTATGG AACAGGGATG CAGACATGGG AGTATTCCCC ATCCTACGCC 
ATTCGTTCCT ATGCGTATCT CTGGCTCCAT GCCCTTCCTG CCTGCTTTCA TGCCAAAATA 
TTACAGACAA ACAAGGTCCT TGTGTTTTAT TTCCTGCGCT GCATGTTAGC GTTTGGCTGT 
TGTGTGTGTG AGCTCTACTT CTATAAGGCT GTGTGTAAGA AATTTGGCCT ACATGTGGGA 
CGGCTTCTGC TGGCGTTCCT CGTCCTGAGT GCAGGGATGT TCTGCTCCTC TGCAGCTTTC 
CTGCCAAGTA CTTTTTGCAT GTACAGTACA GTGGTAGCTA TGACTGGCTG GTTTCAGGGG 
CGTCCGAGTC TGGCTGTGCT GGGTGTCGCA GCAGGGGCCA TTGTGGGCTG GCCCTTCAGT 
GCTCTGCTTG GGATACCCAT TGCCTTTGAC CTTCTGTGGC TGAAGAAGAA ATGGAAGAGT 
TTTATCATGT GGACACTAGT GGCATTGCTG CTCTTTCTGG TCCCTGTGGT TATAGTAGAC 
AGCTACTACT ATGGCAAGCT GGTGATTGCT CCTCTGAATA TAATACTGTA CAATGTATTT 
ACTCCTCATG GGCCAGATCT CTATGGCACA GAGCCCTGGC ATTACTACTT TGTGAATGGT 
TTTCTTAATT TCAACATTGT GTTTGTGTTG GCCCTGTTTT CTCTTCCTCT CACGGCGCTT 
ATGGAGGCAC TGCTTCAAAG ATTCAATGTT CAGAACCTCG GCCGGCCGTA CTGGTTAACT 
TTGTCTCCAA TGTACCTGTG GATGCTGGTC TTCTTCACTC GCCCGCATAA AGAGGAACGC 
TTTCTGTTTC CCATCTACCC GCTCATCTGT CTGTGCGGGG CAGTGGCGCT CTCCTCATTG 
CAGAAGTGCT ATCACTTCAT TTTTCAACGC TATCGTCTGG AGCACTACAC TATCTCCTCC 
AACTGGCTGG CTCTGGGTTC TGTGCTGCTG TTTGGAGTGC TCTCGCTGTC TCGATCTGTG 
GCCCTATTCA GAGGCTATCA TGCTCCGCTG GATTTGTACC CAGAGTTTCA TCGCATTGCT 
AAAGATCCTG CAATCCACAC CGTTCCAGAG GGAAGACTGG TCAATGTTTG TGTCGGGAAA 
GAGTGGTACC GCTTTCCCAG CAGCTTTTTC CTGCCTAACA ACTGGCACCT ACAGTTTATC 
CAGTCAGAAT TCAGGGGTCA GTTACCTAAG CCATATGCCA TGGGTCCTGA TGCCACCTGG 
ATCATCCCAC CAGACATGAA TGATCAGAAT CTTGAGGAGA CATCACGATA CGTAGATTTG 
AAGCAGTGTC ATTACCTGGT GGATCTGGCA ACAGAAACTG AAGCCCCTCG TGAACCACGA 
TATGCCGCGA ACAAAGAGGA GTGGAGCGTT ATTGCTGACA AGCCTTTTCT AGACTCTACC 
AGGTCCTCCA GAATTCTCAG GGCCTTTTTT ATCCCGTTTC TGTCAGAGCA ATACAACACC 
TACAGCAGAT ACGTCATCCT CAAACCGAGG CGACCCAAAC ACACGCGGAA GAGACCGCAG 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300116.1
CDS	16..1941
Translation	MTSRTASRNMATKSLRQRSRRGSKQDANNVNNTPTPENRATKDDKCAEDNKSSDPRNDLLPDKHISSCIPPLSYRSSGKAGQVWAPEGSTAFKCLVSARFCAALLSNLSDCDETFNYWEPTHYLLYGTGMQTWEYSPSYAIRSYAYLWLHALPACFHAKILQTNKVLVFYFLRCMLAFGCCVCELYFYKAVCKKFGLHVGRLLLAFLVLSAGMFCSSAAFLPSTFCMYSTVVAMTGWFQGRPSLAVLGVAAGAIVGWPFSALLGIPIAFDLLWLKKKWKSFIMWTLVALLLFLVPVVIVDSYYYGKLVIAPLNIILYNVFTPHGPDLYGTEPWHYYFVNGFLNFNIVFVLALFSLPLTALMEALLQRFNVQNLGRPYWLTLSPMYLWMLVFFTRPHKEERFLFPIYPLICLCGAVALSSLQKCYHFIFQRYRLEHYTISSNWLALGSVLLFGVLSLSRSVALFRGYHAPLDLYPEFHRIAKDPAIHTVPEGRLVNVCVGKEWYRFPSSFFLPNNWHLQFIQSEFRGQLPKPYAMGPDATWIIPPDMNDQNLEETSRYVDLKQCHYLVDLATETEAPREPRYAANKEEWSVIADKPFLDSTRSSRILRAFFIPFLSEQYNTYSRYVILKPRRPKHTRKRPQA

Target ORF information:

RefSeq Version	XM_009301841.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ALG9, alpha-1,2-mannosyltransferase (alg9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301841.2

ORF Insert Sequence:

ATGACGTCAC GCACAGCCAG TAGAAACATG GCGACGAAGA GTCTCCGTCA GCGCAGCAGA 
CGGGGGAGCA AACAGGACGC CAACAACGTG AACAACACAC CTACCCCTGA AAACCGAGCC 
ACAAAAGACG ACAAATGTGC TGAGGACAAT AAGTCCAGTG ACCCCCGAAA TGATTTGCTC 
CCAGATAAGC ACATTAGCTC CTGTATCCCG CCTCTCTCTT ACAGGTCATC CGGTAAGGCA 
GGTCAGGTTT GGGCTCCTGA GGGCTCCACA GCCTTCAAGT GCCTGGTTTC AGCCCGCTTC 
TGTGCCGCAC TGCTCAGTAA CCTCTCTGAC TGTGATGAAA CCTTCAATTA CTGGGAGCCA 
ACTCACTACT TGCTCTATGG AACAGGGATG CAGACATGGG AGTATTCCCC ATCCTACGCC 
ATTCGTTCCT ATGCGTATCT CTGGCTCCAT GCCCTTCCTG CCTGCTTTCA TGCCAAAATA 
TTACAGACAA ACAAGGTCCT TGTGTTTTAT TTCCTGCGCT GCATGTTAGC GTTTGGCTGT 
TGTGTGTGTG AGCTCTACTT CTATAAGGCT GTGTGTAAGA AATTTGGCCT ACATGTGGGA 
CGGCTTCTGC TGGCGTTCCT CGTCCTGAGT GCAGGGATGT TCTGCTCCTC TGCAGCTTTC 
CTGCCAAGTA CTTTTTGCAT GTACAGTACA GTGGTAGCTA TGACTGGCTG GTTTCAGGGG 
CGTCCGAGTC TGGCTGTGCT GGGTGTCGCA GCAGGGGCCA TTGTGGGCTG GCCCTTCAGT 
GCTCTGCTTG GGATACCCAT TGCCTTTGAC CTTCTGTGGC TGAAGAAGAA ATGGAAGAGT 
TTTATCATGT GGACACTAGT GGCATTGCTG CTCTTTCTGG TCCCTGTGGT TATAGTAGAC 
AGCTACTACT ATGGCAAGCT GGTGATTGCT CCTCTGAATA TAATACTGTA CAATGTATTT 
ACTCCTCATG GGCCAGATCT CTATGGCACA GAGCCCTGGC ATTACTACTT TGTGAATGGT 
TTTCTTAATT TCAACATTGT GTTTGTGTTG GCCCTGTTTT CTCTTCCTCT CACGGCGCTT 
ATGGAGGCAC TGCTTCAAAG ATTCAATGTT CAGAACCTCG GCCGGCCGTA CTGGTTAACT 
TTGTCTCCAA TGTACCTGTG GATGCTGGTC TTCTTCACTC GCCCGCATAA AGAGGAACGC 
TTTCTGTTTC CCATCTACCC GCTCATCTGT CTGTGCGGGG CAGTGGCGCT CTCCTCATTG 
CAGAAGTGCT ATCACTTCAT TTTTCAACGC TATCGTCTGG AGCACTACAC TATCTCCTCC 
AACTGGCTGG CTCTGGGTTC TGTGCTGCTG TTTGGAGTGC TCTCGCTGTC TCGATCTGTG 
GCCCTATTCA GAGGCTATCA TGCTCCGCTG GATTTGTACC CAGAGTTTCA TCGCATTGCT 
AAAGATCCTG CAATCCACAC CGTTCCAGAG GGAAGACTGG TCAATGTTTG TGTCGGGAAA 
GAGTGGTACC GCTTTCCCAG CAGCTTTTTC CTGCCTAACA ACTGGCACCT ACAGTTTATC 
CAGTCAGAAT TCAGGGGTCA GTTACCTAAG CCATATGCCA TGGGTCCTGA TGCCACCTGG 
ATCATCCCAC CAGACATGAA TGATCAGAAT CTTGAGGAGA CATCACGATA CGTAGATTTG 
AAGCAGTGTC ATTACCTGGT GGATCTGGCA ACAGAAACTG AAGCCCCTCG TGAACCACGA 
TATGCCGCGA ACAAAGAGGA GTGGAGCGTT ATTGCTGACA AGCCTTTTCT AGACTCTACC 
AGGTCCTCCA GAATTCTCAG GGCCTTTTTT ATCCCGTTTC TGTCAGAGCA ATACAACACC 
TACAGCAGAT ACGTCATCCT CAAACCGAGG CGACCCAAAC ACACGCGGAA GAGACCGCAG 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

alg9 ( XM_009301841.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300116.1 Danio rerio ALG9, alpha-1,2-mannosyltransferase (alg9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40476
Clone ID Related Accession (Same CDS sequence)	XM_009301841.2 , XM_009301841.3
Accession Version	XM_009301841.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	alpha-1,2-mannosyltransferase ALG9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301841.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCAC GCACAGCCAG TAGAAACATG GCGACGAAGA GTCTCCGTCA GCGCAGCAGA 
CGGGGGAGCA AACAGGACGC CAACAACGTG AACAACACAC CTACCCCTGA AAACCGAGCC 
ACAAAAGACG ACAAATGTGC TGAGGACAAT AAGTCCAGTG ACCCCCGAAA TGATTTGCTC 
CCAGATAAGC ACATTAGCTC CTGTATCCCG CCTCTCTCTT ACAGGTCATC CGGTAAGGCA 
GGTCAGGTTT GGGCTCCTGA GGGCTCCACA GCCTTCAAGT GCCTGGTTTC AGCCCGCTTC 
TGTGCCGCAC TGCTCAGTAA CCTCTCTGAC TGTGATGAAA CCTTCAATTA CTGGGAGCCA 
ACTCACTACT TGCTCTATGG AACAGGGATG CAGACATGGG AGTATTCCCC ATCCTACGCC 
ATTCGTTCCT ATGCGTATCT CTGGCTCCAT GCCCTTCCTG CCTGCTTTCA TGCCAAAATA 
TTACAGACAA ACAAGGTCCT TGTGTTTTAT TTCCTGCGCT GCATGTTAGC GTTTGGCTGT 
TGTGTGTGTG AGCTCTACTT CTATAAGGCT GTGTGTAAGA AATTTGGCCT ACATGTGGGA 
CGGCTTCTGC TGGCGTTCCT CGTCCTGAGT GCAGGGATGT TCTGCTCCTC TGCAGCTTTC 
CTGCCAAGTA CTTTTTGCAT GTACAGTACA GTGGTAGCTA TGACTGGCTG GTTTCAGGGG 
CGTCCGAGTC TGGCTGTGCT GGGTGTCGCA GCAGGGGCCA TTGTGGGCTG GCCCTTCAGT 
GCTCTGCTTG GGATACCCAT TGCCTTTGAC CTTCTGTGGC TGAAGAAGAA ATGGAAGAGT 
TTTATCATGT GGACACTAGT GGCATTGCTG CTCTTTCTGG TCCCTGTGGT TATAGTAGAC 
AGCTACTACT ATGGCAAGCT GGTGATTGCT CCTCTGAATA TAATACTGTA CAATGTATTT 
ACTCCTCATG GGCCAGATCT CTATGGCACA GAGCCCTGGC ATTACTACTT TGTGAATGGT 
TTTCTTAATT TCAACATTGT GTTTGTGTTG GCCCTGTTTT CTCTTCCTCT CACGGCGCTT 
ATGGAGGCAC TGCTTCAAAG ATTCAATGTT CAGAACCTCG GCCGGCCGTA CTGGTTAACT 
TTGTCTCCAA TGTACCTGTG GATGCTGGTC TTCTTCACTC GCCCGCATAA AGAGGAACGC 
TTTCTGTTTC CCATCTACCC GCTCATCTGT CTGTGCGGGG CAGTGGCGCT CTCCTCATTG 
CAGAAGTGCT ATCACTTCAT TTTTCAACGC TATCGTCTGG AGCACTACAC TATCTCCTCC 
AACTGGCTGG CTCTGGGTTC TGTGCTGCTG TTTGGAGTGC TCTCGCTGTC TCGATCTGTG 
GCCCTATTCA GAGGCTATCA TGCTCCGCTG GATTTGTACC CAGAGTTTCA TCGCATTGCT 
AAAGATCCTG CAATCCACAC CGTTCCAGAG GGAAGACTGG TCAATGTTTG TGTCGGGAAA 
GAGTGGTACC GCTTTCCCAG CAGCTTTTTC CTGCCTAACA ACTGGCACCT ACAGTTTATC 
CAGTCAGAAT TCAGGGGTCA GTTACCTAAG CCATATGCCA TGGGTCCTGA TGCCACCTGG 
ATCATCCCAC CAGACATGAA TGATCAGAAT CTTGAGGAGA CATCACGATA CGTAGATTTG 
AAGCAGTGTC ATTACCTGGT GGATCTGGCA ACAGAAACTG AAGCCCCTCG TGAACCACGA 
TATGCCGCGA ACAAAGAGGA GTGGAGCGTT ATTGCTGACA AGCCTTTTCT AGACTCTACC 
AGGTCCTCCA GAATTCTCAG GGCCTTTTTT ATCCCGTTTC TGTCAGAGCA ATACAACACC 
TACAGCAGAT ACGTCATCCT CAAACCGAGG CGACCCAAAC ACACGCGGAA GAGACCGCAG 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300116.1
CDS	17..1942
Translation	MTSRTASRNMATKSLRQRSRRGSKQDANNVNNTPTPENRATKDDKCAEDNKSSDPRNDLLPDKHISSCIPPLSYRSSGKAGQVWAPEGSTAFKCLVSARFCAALLSNLSDCDETFNYWEPTHYLLYGTGMQTWEYSPSYAIRSYAYLWLHALPACFHAKILQTNKVLVFYFLRCMLAFGCCVCELYFYKAVCKKFGLHVGRLLLAFLVLSAGMFCSSAAFLPSTFCMYSTVVAMTGWFQGRPSLAVLGVAAGAIVGWPFSALLGIPIAFDLLWLKKKWKSFIMWTLVALLLFLVPVVIVDSYYYGKLVIAPLNIILYNVFTPHGPDLYGTEPWHYYFVNGFLNFNIVFVLALFSLPLTALMEALLQRFNVQNLGRPYWLTLSPMYLWMLVFFTRPHKEERFLFPIYPLICLCGAVALSSLQKCYHFIFQRYRLEHYTISSNWLALGSVLLFGVLSLSRSVALFRGYHAPLDLYPEFHRIAKDPAIHTVPEGRLVNVCVGKEWYRFPSSFFLPNNWHLQFIQSEFRGQLPKPYAMGPDATWIIPPDMNDQNLEETSRYVDLKQCHYLVDLATETEAPREPRYAANKEEWSVIADKPFLDSTRSSRILRAFFIPFLSEQYNTYSRYVILKPRRPKHTRKRPQA

Target ORF information:

RefSeq Version	XM_009301841.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ALG9, alpha-1,2-mannosyltransferase (alg9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301841.3

ORF Insert Sequence:

ATGACGTCAC GCACAGCCAG TAGAAACATG GCGACGAAGA GTCTCCGTCA GCGCAGCAGA 
CGGGGGAGCA AACAGGACGC CAACAACGTG AACAACACAC CTACCCCTGA AAACCGAGCC 
ACAAAAGACG ACAAATGTGC TGAGGACAAT AAGTCCAGTG ACCCCCGAAA TGATTTGCTC 
CCAGATAAGC ACATTAGCTC CTGTATCCCG CCTCTCTCTT ACAGGTCATC CGGTAAGGCA 
GGTCAGGTTT GGGCTCCTGA GGGCTCCACA GCCTTCAAGT GCCTGGTTTC AGCCCGCTTC 
TGTGCCGCAC TGCTCAGTAA CCTCTCTGAC TGTGATGAAA CCTTCAATTA CTGGGAGCCA 
ACTCACTACT TGCTCTATGG AACAGGGATG CAGACATGGG AGTATTCCCC ATCCTACGCC 
ATTCGTTCCT ATGCGTATCT CTGGCTCCAT GCCCTTCCTG CCTGCTTTCA TGCCAAAATA 
TTACAGACAA ACAAGGTCCT TGTGTTTTAT TTCCTGCGCT GCATGTTAGC GTTTGGCTGT 
TGTGTGTGTG AGCTCTACTT CTATAAGGCT GTGTGTAAGA AATTTGGCCT ACATGTGGGA 
CGGCTTCTGC TGGCGTTCCT CGTCCTGAGT GCAGGGATGT TCTGCTCCTC TGCAGCTTTC 
CTGCCAAGTA CTTTTTGCAT GTACAGTACA GTGGTAGCTA TGACTGGCTG GTTTCAGGGG 
CGTCCGAGTC TGGCTGTGCT GGGTGTCGCA GCAGGGGCCA TTGTGGGCTG GCCCTTCAGT 
GCTCTGCTTG GGATACCCAT TGCCTTTGAC CTTCTGTGGC TGAAGAAGAA ATGGAAGAGT 
TTTATCATGT GGACACTAGT GGCATTGCTG CTCTTTCTGG TCCCTGTGGT TATAGTAGAC 
AGCTACTACT ATGGCAAGCT GGTGATTGCT CCTCTGAATA TAATACTGTA CAATGTATTT 
ACTCCTCATG GGCCAGATCT CTATGGCACA GAGCCCTGGC ATTACTACTT TGTGAATGGT 
TTTCTTAATT TCAACATTGT GTTTGTGTTG GCCCTGTTTT CTCTTCCTCT CACGGCGCTT 
ATGGAGGCAC TGCTTCAAAG ATTCAATGTT CAGAACCTCG GCCGGCCGTA CTGGTTAACT 
TTGTCTCCAA TGTACCTGTG GATGCTGGTC TTCTTCACTC GCCCGCATAA AGAGGAACGC 
TTTCTGTTTC CCATCTACCC GCTCATCTGT CTGTGCGGGG CAGTGGCGCT CTCCTCATTG 
CAGAAGTGCT ATCACTTCAT TTTTCAACGC TATCGTCTGG AGCACTACAC TATCTCCTCC 
AACTGGCTGG CTCTGGGTTC TGTGCTGCTG TTTGGAGTGC TCTCGCTGTC TCGATCTGTG 
GCCCTATTCA GAGGCTATCA TGCTCCGCTG GATTTGTACC CAGAGTTTCA TCGCATTGCT 
AAAGATCCTG CAATCCACAC CGTTCCAGAG GGAAGACTGG TCAATGTTTG TGTCGGGAAA 
GAGTGGTACC GCTTTCCCAG CAGCTTTTTC CTGCCTAACA ACTGGCACCT ACAGTTTATC 
CAGTCAGAAT TCAGGGGTCA GTTACCTAAG CCATATGCCA TGGGTCCTGA TGCCACCTGG 
ATCATCCCAC CAGACATGAA TGATCAGAAT CTTGAGGAGA CATCACGATA CGTAGATTTG 
AAGCAGTGTC ATTACCTGGT GGATCTGGCA ACAGAAACTG AAGCCCCTCG TGAACCACGA 
TATGCCGCGA ACAAAGAGGA GTGGAGCGTT ATTGCTGACA AGCCTTTTCT AGACTCTACC 
AGGTCCTCCA GAATTCTCAG GGCCTTTTTT ATCCCGTTTC TGTCAGAGCA ATACAACACC 
TACAGCAGAT ACGTCATCCT CAAACCGAGG CGACCCAAAC ACACGCGGAA GAGACCGCAG 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

trappc11 is required for protein glycosylation in zebrafish and humans.
Molecular biology of the cell27(8)1220-34(2016 Apr)
DeRossi C,Vacaru A,Rafiq R,Cinaroglu A,Imrie D,Nayar S,Baryshnikova A,Milev MP,Stanga D,Kadakia D,Gao N,Chu J,Freeze HH,Lehrman MA,Sacher M,Sadler KC

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish miR-1 and miR-133 shape muscle gene expression and regulate sarcomeric actin organization.
Genes & development23(5)619-32(2009 Mar)
Mishima Y,Abreu-Goodger C,Staton AA,Stahlhut C,Shou C,Cheng C,Gerstein M,Enright AJ,Giraldez AJ

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alg9 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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