ncs1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ncs1a
Entrez Gene ID	393437
Full Name	neuronal calcium sensor 1a
Synonyms	freq,freqa,zgc:63620
General protein information	Preferred Names neuronal calcium sensor 1a Names neuronal calcium sensor 1 frequenin homolog a ncs-1a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001328512.1	NP_001315441.1	neuronal calcium sensor 1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ncs1a	NP_956759.1
Pan troglodytes (chimpanzee)	NCS1	XP_001165499.2
Macaca mulatta (Rhesus monkey)	LOC722507	XP_001118646.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Drug screening in Scn1a zebrafish mutant identifies clemizole as a potential Dravet syndrome treatment.
Baraban SC, Dinday MT, Hortopan GA
Nature communications42410(2013)

Proteomic and functional analysis of NCS-1 binding proteins reveals novel signaling pathways required for inner ear development in zebrafish.
Petko JA, Kabbani N, Frey C, Woll M, Hickey K, Craig M, Canfield VA, Levenson R
BMC neuroscience1027(2009 Mar)

Functional and pharmacological characterization of a Shal-related K+ channel subunit in Zebrafish.
Nakamura TY, Coetzee WA
BMC physiology82(2008 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ncs1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ncs1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa40446	NM_001328512.1 Latest version!	Danio rerio neuronal calcium sensor 1a (ncs1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ncs1a ( NM_001328512.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001315441.1 Danio rerio neuronal calcium sensor 1a (ncs1a), mRNA.

Price & Availability↑

CloneID	ODa40446
Clone ID Related Accession (Same CDS sequence)	NM_001328512.1
Accession Version	NM_001328512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548432000000
Organism	Danio rerio(zebrafish)
Product	neuronal calcium sensor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FO704774.1, AY190280.1 and BX510649.12. On Jun 16, 2016 this sequence version replaced XM_009301454.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01035716.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGGCAAAT CCAACAGCAA ACTAAAGCCT GAGGTAGTGG AAGATTTGTG CAGAAAGACC 
TACTTCACAG AGAAGGAAGT ACAGCAGTGG TATAAGGGTT TTATTAAAGA CTGCCCCAGT 
GGTCAACTGG ACTCCTCTGG CTTTCAGAAG ATCTACAAGC AGTTCTTCCC TTTCGGTGAT 
CCAACCAAAT TTGCCACATT CGTCTTCAAT GTCTTTGACG AGAACAAGGA TGGACGCATT 
GAGTTCTCAG AGTTTATTCA AGCTCTGTCT GTCACATCAA GAGGAACACT GGATGAAAAA 
CTCAGATGGG CCTTTAAACT TTACGATCTT GATAATGATG GCTACATAAC TCGCGATGAG 
ATGCTGAACA TCGTGGACGC CATCTACCAG ATGGTGGGGA ACACAGTGGA GTTACCTGAA 
GAGGAAAACA CACCAGAAAA ACGAGTTGAT CGCATCTTTG CTATGATGGA TAAGAATGCA 
GATGGGATGT TGACTCTACA GGAGTTTCAG GAGGGCTCTA AGGCTGACCC GTCTATTGTA 
CAGGCACTGT CTCTTTATGA CGGATTGGTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315441.1
CDS	548..1120
Translation	MGKSNSKLKPEVVEDLCRKTYFTEKEVQQWYKGFIKDCPSGQLDSSGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRDEMLNIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGMLTLQEFQEGSKADPSIVQALSLYDGLV

Target ORF information:

RefSeq Version	NM_001328512.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuronal calcium sensor 1a (ncs1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328512.1

ORF Insert Sequence:

ATGGGCAAAT CCAACAGCAA ACTAAAGCCT GAGGTAGTGG AAGATTTGTG CAGAAAGACC 
TACTTCACAG AGAAGGAAGT ACAGCAGTGG TATAAGGGTT TTATTAAAGA CTGCCCCAGT 
GGTCAACTGG ACTCCTCTGG CTTTCAGAAG ATCTACAAGC AGTTCTTCCC TTTCGGTGAT 
CCAACCAAAT TTGCCACATT CGTCTTCAAT GTCTTTGACG AGAACAAGGA TGGACGCATT 
GAGTTCTCAG AGTTTATTCA AGCTCTGTCT GTCACATCAA GAGGAACACT GGATGAAAAA 
CTCAGATGGG CCTTTAAACT TTACGATCTT GATAATGATG GCTACATAAC TCGCGATGAG 
ATGCTGAACA TCGTGGACGC CATCTACCAG ATGGTGGGGA ACACAGTGGA GTTACCTGAA 
GAGGAAAACA CACCAGAAAA ACGAGTTGAT CGCATCTTTG CTATGATGGA TAAGAATGCA 
GATGGGATGT TGACTCTACA GGAGTTTCAG GAGGGCTCTA AGGCTGACCC GTCTATTGTA 
CAGGCACTGT CTCTTTATGA CGGATTGGTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Drug screening in Scn1a zebrafish mutant identifies clemizole as a potential Dravet syndrome treatment.
Nature communications42410(2013)
Baraban SC,Dinday MT,Hortopan GA

Proteomic and functional analysis of NCS-1 binding proteins reveals novel signaling pathways required for inner ear development in zebrafish.
BMC neuroscience1027(2009 Mar)
Petko JA,Kabbani N,Frey C,Woll M,Hickey K,Craig M,Canfield VA,Levenson R

Functional and pharmacological characterization of a Shal-related K+ channel subunit in Zebrafish.
BMC physiology82(2008 Feb)
Nakamura TY,Coetzee WA